LOCUS       DASZMB010000001         3086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3024570, whole genome shotgun sequence.
ACCESSION   DASZMB010000001 DASZMB010000000
VERSION     DASZMB010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3024570"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1383
                     /locus_tag="VG272_00005"
     CDS             <1..1383
                     /locus_tag="VG272_00005"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWF12089.1"
                     /translation="AAISAINQANINYFFMKPWDPPSEHLYPQLDDLLDDWKASYHPE
                     FQGIRVLGTRWSPKCYELRDFLARNHVPYQWIDAEVSANDPETKRLMEVLGPEATSLP
                     VVLFPDGSRLLQSAPADVAQKVGLRTHAETSFYDLAIVGGGPAGLAAAVYGASEGLHT
                     VMIEREAPGGQAGMSSRIENYLGFPNGLSGGDLARRAVVQARRFGVEILAPQEAVAVR
                     TEGSYRIIKLPDGSEISCHALMIATGVQWRRLNAPGVDRLQGAGVYYGGGATEALSCK
                     GEIIYVVGGANSAGQAAMNFAKYAERVVILVRGNSLSSTMSQYLIDQIKQTSNIQVWA
                     NASLVEAHGDTHLEEISVLCSDTNKIERVPAGSAFIFIGAVPRTDWLGDLLERDERGF
                     LLAGPDLIREGQRPKGWTLERDPYLLETNVPGIFAVGDVRHGSVKRVASGVGEGSVAV
                     QFIHQYLAKV"
     gene            1404..2789
                     /locus_tag="VG272_00010"
     CDS             1404..2789
                     /locus_tag="VG272_00010"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF12090.1"
                     /translation="MVENFELLRVPVFSDLPEDQIAWFISQSQEMKLSEGQMALNAND
                     PADAMFVILEGTVQGRGELGGETIVFDIPQGSVTGRLPFSRMKQFTISARAITDARVL
                     RFPSSQFQELVQKMPELAARLVGLMSDRIRETTRMEQQRDRLAALGKLSAGLAHELNN
                     PASAAKRAASQLRDLLKRIRDASHALGRRELTPAQKSEIEKLEAAITHYNEPPPDALT
                     LSELEGELDSLLRSHKQSDLWQLAGDLARKNVRPATLESLFATLDEGTARAALLRIAA
                     SVEIAELLKEIESSTSRISDLVGAIKEYTYMDQSPVQNVDIVKGLETTLTILNHKLKH
                     GVTVQRDYQRIPLLVNSFGSELNQIWTNLIDNAIDAMNGKGELRVRTYRDGPCVVVEI
                     ADNGPGIPDGIREHIFEPFFTTKAVGEGTGLGLDTVQRIVKKHRGTIQVTSKPGETRF
                     QVFLPLSEKMA"
BASE COUNT          702 a          828 c          910 g          646 t
ORIGIN      
        1 gccgccatca gcgccatcaa tcaggccaac atcaattatt ttttcatgaa gccatgggat
       61 cctccctccg aacacttgta tccgcaattg gatgatttgc ttgacgattg gaaggcttcc
      121 tatcatccgg aatttcaggg cattcgggtt ttgggaacac gctggtcgcc gaaatgttac
      181 gaactgcggg attttctggc ccgcaatcac gtcccctatc agtggatcga tgcggaggtt
      241 tccgcgaacg atccggaaac aaaacgcctg atggaagtgc tgggaccaga ggcaaccagc
      301 ctgcccgtgg tgctgtttcc ggatggctcc aggctgctgc agagcgctcc cgcggatgtg
      361 gcgcaaaaag taggattgcg gactcatgca gagaccagct tttacgattt ggcgatcgtg
      421 ggcggaggtc ccgcgggatt ggcggccgca gtttacggcg cttccgaagg tttgcacacc
      481 gtaatgattg agcgcgaagc tccgggagga caggccggga tgagctcgcg catcgaaaat
      541 tacttgggat ttcccaatgg actgagcgga ggcgacctcg caagacgcgc cgtggtgcaa
      601 gcgcgccgct tcggagtgga aatcctggct ccgcaggaag cggtagcggt caggaccgag
      661 ggatcgtatc gaatcatcaa attgccggac ggcagcgaaa tttcctgtca cgcgctgatg
      721 attgccacgg gcgtgcagtg gcggcggctg aacgctcccg gagtcgaccg gttgcagggc
      781 gccggcgtat attatggcgg cggcgcaacc gaggctttgt cctgcaaggg tgaaattatt
      841 tatgtggtag gcggagccaa ttcggcagga caggccgcga tgaatttcgc caagtatgcc
      901 gaacgtgtcg tgatcctggt gcgcggcaat tccctttcca gcaccatgtc gcaatattta
      961 atcgatcaga tcaaacagac gtccaacatt caagtttggg ccaatgccag cctggtagaa
     1021 gcgcacggag atacgcacct ggaggaaatt tccgtgctat gttcagacac aaataaaatc
     1081 gagcgcgtgc cggcggggtc cgcattcatc tttatcggcg ctgtgccgcg caccgattgg
     1141 ttgggcgatt tgctggagag ggacgagcgg ggattcctgc tcgcgggccc cgatttgata
     1201 cgcgagggcc agcgtccaaa aggttggacg ctggagcgcg atccctattt gctggaaacc
     1261 aacgtgccgg gcatttttgc ggtgggtgac gtacggcacg gttcggtaaa acgcgtagca
     1321 tcgggcgtcg gcgagggatc ggtggccgtg cagttcattc atcagtattt ggcaaaggtc
     1381 taagtaatcc ggcgaaggtg taaatggtag aaaatttcga attacttcgc gttccggtat
     1441 tctccgatct gcccgaggat caaatcgcct ggtttatcag ccagtcgcaa gaaatgaaac
     1501 tcagcgaagg ccagatggcg ctgaacgcaa acgatccggc cgatgccatg tttgtgatac
     1561 tggaaggcac ggtgcagggg cggggcgaac tcggcggcga aaccattgtt ttcgatatac
     1621 cgcagggcag cgttaccgga cgtctgccgt tttccagaat gaagcagttt acgatttccg
     1681 cgcgtgccat taccgacgcc cgtgtgctgc gctttccatc ttcgcaattt caagagttgg
     1741 tacaaaaaat gccggagctg gccgcgcggc tggtcggact aatgtccgat cgaattcgcg
     1801 agaccacacg catggagcag cagcgcgacc gccttgccgc cttgggaaag ctctcggcag
     1861 ggctggcgca cgagctgaat aatcccgcct cggccgccaa acgcgccgcc agccagttgc
     1921 gtgacctact aaaacgtatt cgcgacgcaa gccacgccct gggacgccgc gaactcaccc
     1981 cggcgcaaaa gtctgaaatt gagaaactgg aagcagccat cacccactat aacgagccgc
     2041 cacccgatgc cttgacgctt agcgagctgg aaggtgagct ggactctctg ctgcgcagcc
     2101 acaagcagag cgatctatgg cagttggcgg gggatttggc gcgcaagaac gtccggccgg
     2161 ccactctgga atcgttattt gcgaccctgg atgaaggcac ggctcgggct gcgctcttgc
     2221 ggattgcggc ttcggtggaa attgccgaat tgctgaagga aatcgaaagc agcacgtcgc
     2281 gcatttccga tctggtcggt gcgatcaagg aatacaccta catggaccag tcaccggtcc
     2341 agaatgtgga tatcgtcaag ggactcgaaa ccacgctcac catcctgaat cacaagctca
     2401 agcacggcgt gaccgtgcaa cgcgattatc agcgtattcc gctgctggta aattccttcg
     2461 gcagcgaact caaccaaatc tggaccaacc ttatcgacaa cgccattgac gcgatgaacg
     2521 gtaagggcga gttgcgcgtt cggacttacc gcgacggccc ttgcgtcgtt gtggaaatag
     2581 ccgataacgg ccccggaatt ccggatggga ttcgagagca catttttgag ccctttttca
     2641 ccacaaaggc cgtcggagaa ggaaccggct tgggcctgga taccgtgcaa cggatcgtta
     2701 aaaagcatcg tggaacgatt caagtaacat caaagcccgg cgaaactcgt tttcaggtat
     2761 tccttccatt gtcggaaaaa atggcttaga tcctcaacct gaagccggga ctggaacggc
     2821 tgtagcccca cctattagga tccgagtcct tcgaccgttc gacccgcgtt cacaaagcgc
     2881 agatcccttt cggtggccta attccacatt gcgcgaaaga aaaatactcg ataggctctc
     2941 ggtggactca ggcgacagag gcttccgccg gtgtgctgtt acttcgctga ataatccgag
     3001 ctatggcttg cactcatgga ggccagaaac aattgagagt tttgcccaga gacgattcgt
     3061 gagcagtcat cttttgcaga tactac
//
LOCUS       DASZMB010000002         3283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_277232, whole genome shotgun sequence.
ACCESSION   DASZMB010000002 DASZMB010000000
VERSION     DASZMB010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277232"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="VG272_00015"
     CDS             <1..863
                     /locus_tag="VG272_00015"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HWF12091.1"
                     /translation="AKTAIALAIAGSEASGLRDNFGSMTKPFHAGHAGENGTVAVDLA
                     AIGWTAASDILEAELGFFAVGGGFDPKFIVDRLGKPWMFASPGDLIKRFPCGTIQQQV
                     MDEMLRLIQQNNIKAADVEKVEVGGNLSNYRTLFQHHPTTGLQGKFSMEYAMALLLLD
                     GKATLTSFTDAVAQRPDMQDMIKRVRYYVDPEFNKLELQGASLQAMLVEQSMLKIYMK
                     NGKVIAGRTEPAKGSPENPMTFEEVADKFRGNAEFLKWPSQKAETVIELVKSLETVPD
                     MSKLTAALAG"
     gene            945..1859
                     /locus_tag="VG272_00020"
     CDS             945..1859
                     /locus_tag="VG272_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12092.1"
                     /translation="MIRRMKIFLAACLSASCFAIALSSLPMAAADDEIITLDKAATAA
                     LSKGDKAAQGKYLDADFSWVDTKGVMRFGDDSFRAGIKPAVPVADDVKYIEHKYGNVV
                     WLQSNQGKRYSAHFWVKRPAGWKLLHMTEIDVHERDFSTVRPNYEVPCINPCKAVPYT
                     ATTANEKAALDGWQEQESGREGMWAKHIADNFDQRAAMTWSGPRASKTEMVAMQARAR
                     QQSSGKPEVGTAPALFIRSWDFGTAVVMISCQPTYGDKAYWSSRVLAPLDGVWMMMES
                     YHNYIDASPVMTAVPIDQSKDPRAAAMK"
     gene            complement(2164..3051)
                     /locus_tag="VG272_00025"
     CDS             complement(2164..3051)
                     /locus_tag="VG272_00025"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWF12093.1"
                     /translation="MLVFLTGATGYIGSAVAEVLQKAGHKVTGLARTPEKAKELETRG
                     IRAHLGDLLKPETVASAARTADGVIHTANTNDANSAPADLAVVRAMIAALEGSGKPFV
                     YTSGVWVLGSTGDRIATEQTPVNPTPLAARRPAIEQEVLAAQDRGVRAIVIRPALVYG
                     RGGSIPAMLTKSARETGAARFVGDGQNRWPFVNVEDLAQLYVRAVEKAAAGSLYNASH
                     GPSYRAGEVAEAASIGAGANGKTQSWPLEEARKTIGAFADALVLDQQVSGEKAMKELG
                     WAPRAGSVLDDLKTGSYAH"
BASE COUNT          765 a          912 c          870 g          736 t
ORIGIN      
        1 cggcaaaaac cgccattgct ctggccattg ccggatcgga agccagcgga cttcgcgaca
       61 attttggctc catgacgaag ccctttcacg ctgggcatgc cggcgaaaac ggaaccgtag
      121 ctgtcgatct tgcggcgatc ggctggaccg ctgcgtcaga tattctcgaa gcggaactcg
      181 gattcttcgc cgtcggcggc ggattcgatc cgaaatttat cgtggatcgc ctcggaaagc
      241 cgtggatgtt tgcttcgccc ggcgatctga tcaaacgatt cccctgcggc acgattcagc
      301 aacaagtgat ggacgaaatg ctgcgtctca ttcagcaaaa caacatcaag gccgcggatg
      361 tcgagaaagt cgaagtcggc ggaaacctga gcaattaccg aacgcttttc cagcaccatc
      421 ccaccactgg cctgcaaggg aaattcagta tggaatatgc catggccctc ttgctgctcg
      481 atggcaaagc tacgctcact tcgttcacgg atgccgttgc gcagcgcccc gacatgcagg
      541 atatgatcaa gcgcgtgcgc tattacgtcg atcccgaatt caacaagctg gagctgcagg
      601 gagcttcttt gcaagccatg ctcgtcgaac agagcatgct aaaaatttac atgaaaaacg
      661 gcaaggtgat tgcgggccgc acggaacctg cgaagggaag cccggaaaac ccaatgacgt
      721 ttgaggaagt ggccgacaaa ttccgcggca acgcggaatt tttgaaatgg ccttcgcaaa
      781 aagcggagac cgtgatcgaa cttgtgaagt cgctcgaaac ggtgcccgac atgtccaaac
      841 tcaccgctgc tttggcgggt tgaaatctgt taaaggcaaa atgctttcgt tgcagttgac
      901 gttgatgttg aaaagctatc gttaaaataa tagggagaat aaaaatgatc cgacgtatga
      961 aaatttttct cgctgcatgt ttaagcgcca gctgtttcgc gattgcctta tccagcctgc
     1021 ctatggctgc tgcggatgat gaaatcatca ctctcgacaa agccgcgacc gcggccctct
     1081 caaagggcga caaagcggcg cagggcaaat atctggacgc cgacttcagc tgggttgata
     1141 cgaaaggggt gatgcgtttc ggcgatgatt cttttcgcgc cggaatcaag cctgccgttc
     1201 cggtggccga tgacgtgaaa tacatcgagc acaagtacgg caacgtcgtc tggcttcaat
     1261 cgaatcaggg caagcgctac tcggcgcatt tttgggtgaa gcgccccgcg ggttggaagc
     1321 tgctgcacat gaccgaaatc gatgttcacg aacgagactt ttcaaccgtg cgtccgaatt
     1381 acgaagtgcc atgcatcaat ccttgcaagg ccgttcctta tactgcaacc acagccaatg
     1441 aaaaggcggc gctagacggt tggcaggaac aggaatccgg cagggaagga atgtgggcca
     1501 agcacatcgc cgacaatttc gatcagcgcg cggcgatgac gtggagcgga ccaagagcat
     1561 ccaaaacgga aatggttgca atgcaagcgc gcgccaggca acagagctcg ggcaagcctg
     1621 aagtgggtac ggctcctgct cttttcattc gctcttggga ttttggaaca gccgttgtga
     1681 tgatttcctg ccagccgacg tacggcgaca aggcatattg gtccagccgc gtgcttgccc
     1741 ctctcgatgg ggtgtggatg atgatggaga gctatcacaa ttacatcgat gcttcgcccg
     1801 tcatgaccgc cgtgcctatc gaccagtcga aagatccgcg cgcagctgcc atgaaataat
     1861 cttgtcttta gaagttgtct ggctctgtag acatcccggt tccgctgctg atctggcgca
     1921 gcccgcctct tttcacgcgg ggtttttgga gctccagtat ctaaaccctc gcctctgaag
     1981 aggcgagcta cgattccagc gccacgaaat cggcgaaact ttctgaagca tccaggatgt
     2041 caacagatcc atgccatttt ataaatattt tttgtgggtc gcggcttcaa tcgcgacgat
     2101 caggggcgcg gtccaatgtg gtttggaaaa aaatcacatg cgaagtttca aaaaattata
     2161 aatttagtgc gcgtacgatc ctgttttcaa gtcatccagc acggaccccg cgcgcggcgc
     2221 ccagcctaac tctttcatag ctttttcacc gcttacttgt tgatcgagaa caagcgcatc
     2281 cgcaaatgcc ccaatcgttt tacgcgcctc ctcaagcggc caggattgcg tttttccatt
     2341 cgctcccgcg ccaatacttg cagcttcggc aacttcaccg gcgcgatacg acggcccatg
     2401 cgacgcgttg tagagcgaac ctgcagcggc cttttccact gcccgaacat aaagttgcgc
     2461 aaggtcttcc acgttcacaa acggccaccg gttttggccg tctccaacaa aacgggcggc
     2521 tccggtttcg cgagcggatt tcgtaagcat cgccggaata cttccgccgc gcccgtaaac
     2581 caacgcggga cgaatcacaa tcgcgcgcac gccgcggtct tgcgccgcca gaacttcctg
     2641 ctcaatcgcg ggacggcgtg cggccagcgg agtgggattc acgggagttt gttcggttgc
     2701 tatgcgatcc ccagttgaac ccaacaccca cacgccactt gtatagacaa aaggctttcc
     2761 gcttccttcc agcgccgcga tcatggcgcg caccacggcc aaatccgctg gcgcagagtt
     2821 tgcatcgttc gtattcgccg tgtgaatcac gccatccgcg gtgcgcgctg ccgaggcaac
     2881 ggtttcgggt ttaagaaggt cgcccaggtg agctcgaatc ccccgcgtct ccagttcctt
     2941 cgctttttcc ggcgtgcgcg caaggccggt tactttatgg cccgctttct gcaacacttc
     3001 ggcaacagcg cttccaatat atcccgtagc tccggtcagg aaaaccaaca tgggatgatt
     3061 gcctccaaaa tcattttact tcgatgcgat ggaaactcaa gtttcgtacg cgttgcacgg
     3121 ataaagacga ggagaatttt tgcaatgggg gactacggtg ccgttgagaa aattccagaa
     3181 atcacttttc ctacatttaa attctaagtc gacggctact ttttcttttc ggatggagca
     3241 agcaccgcgt tcccaacctg cttgttatac tccgccatat cga
//
LOCUS       DASZMB010000003         4256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3577143, whole genome shotgun sequence.
ACCESSION   DASZMB010000003 DASZMB010000000
VERSION     DASZMB010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3577143"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1429)
                     /locus_tag="VG272_00030"
     CDS             complement(<1..1429)
                     /locus_tag="VG272_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12094.1"
                     /translation="MIDERWRGCDWAAAAFLFLSTAAVVLWQNSRLAVLWDLSYILEN
                     SYRISLGQLPYRDFPFPYAPGTFLVQALLIKLTGRVFFHHVLYCAVIGGLGTVVTWRI
                     LLRVLREPTVKFRLIAFLLTLPITVLGIYSVFPHPFYDPDCTFVILLCVFFLFKLESK
                     NFPAWPTFSAGIVFVVPIFVKQNTGLAFVAGVKWVILLFIVHGIWRKRRVAGYFWLLA
                     GMTAGFSVALLALHFTVGLKNYWHWTIQFAASRRLPGIAEMFAPYMNPMLLLWIAAFA
                     AGAWLLWYGNRRGKPMLSRSAVAFISVPFGWTIVYLFLDDDPSERAERLLALWPLVLL
                     TSLFIALWNLKKGPTIARLLPFILIATVQGAFFSQQLWGSTYAIWPLLMILFAGILAE
                     FFRAENTNAVSEVQWLAGIAALSMLVAGSFYVVNHERLDYSNVSDGDLSHPTRPELKG
                     LSMRGDWVPQFEELVHFTDRAIPRNE"
     gene            complement(1636..2538)
                     /locus_tag="VG272_00035"
     CDS             complement(1636..2538)
                     /locus_tag="VG272_00035"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWF12095.1"
                     /translation="MSIRPVKRLVKSKPTREGAGVHLRRAFGFGNTSEYDPFLLLDDF
                     RNDVPEDYLAGFPWHPHRGIETITYVLAGTVDHGDSLGNQGSIAPGDVQWMTAGSGII
                     HQEMPKGDATGRMHGFQLWANLPSSLKMTAPRYQGVEAKDIPNITDDDGTHVRLVCGK
                     FWGKSGPVDGVAADPIYLDVSVPPGQRKTLPVETTRHAFAYVFAGSGKFCNASEPLAV
                     PTEGVGWADTTPPTSADNRSLVLFDQGDEVAVQAGDEGVRFLLVSGKPLEEPVAWYGP
                     IVMNTQSQLQKAFQELQEGTFLKK"
     gene            complement(2601..2810)
                     /locus_tag="VG272_00040"
     CDS             complement(2601..2810)
                     /locus_tag="VG272_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12096.1"
                     /translation="MASKSKLKDAAVKIGSVVGKVDGTAHKAARKTSDAIKIARRELD
                     DVTKQVEALKKQLAKSSKRLQDSLR"
     gene            3096..3347
                     /locus_tag="VG272_00045"
     CDS             3096..3347
                     /locus_tag="VG272_00045"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HWF12097.1"
                     /translation="MSFIAWIILGLIAGFVGSKLVNHQGEGIMLDIVLGVVGAIVGGW
                     LFTKFGAQGVTGLNLYSIVVAVVGAVVFLVAYHAIRRAV"
     gene            3457..3978
                     /locus_tag="VG272_00050"
     CDS             3457..3978
                     /locus_tag="VG272_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWF12098.1"
                     /translation="MGIFDKMFGSEVKAALAEPNAQQRFDQLKQKYQSVLNLIQQENV
                     QLSNLHVQADKLFIRGTAPSEAVKNKIWDEIKLVNPKSDDIIANLTVRPQAAQAAAAG
                     ATSQTGAGPHIESGQDQTYMVQPGDSLSRIADRFYGSAHEYMKIYYANKDQISNPDQI
                     RAGMKLNIPVKNG"
BASE COUNT         1044 a         1165 c         1163 g          884 t
ORIGIN      
        1 cctcgttccg gggaattgcg cgatccgtga aatgcacaag ttcctcaaat tgcggaaccc
       61 agtcgccacg catcgagagt cccttcagtt caggccgcgt cggatgagaa aggtcaccgt
      121 cagaaacgtt cgagtagtcg aggcgctcgt gattcaccac gtaaaatgag ccggcaacga
      181 gcatcgagag cgccgcgatt ccagcgagcc attgcacttc actgacagcg tttgtatttt
      241 ccgcacgaaa aaattccgcg agaatgcccg caaataaaat catgagcagc ggccagatgg
      301 cgtacgtgga gccccaaagc tgctgcgaga agaacgcgcc ctgcaccgtg gcgattagaa
      361 taaatgggag aagccgcgcg atcgtcggac cttttttcaa attccaaagt gcgataaaaa
      421 gagaagtgag caaaaccagg ggccacagcg cgaggaggcg ctccgcgcgc tcggaaggat
      481 catcgtccag gaataaataa acgatggtcc acccgaaagg aacgctaatg aatgcgacgg
      541 cgcttcgcga aagcatcggt ttgccgcggc gattgccata ccacagcaac catgcgccgg
      601 cggcaaacgc cgcgatccac aggagcagca tcggattcat gtacggcgcg aacatttccg
      661 cgataccggg caatcttcgc gacgcggcaa actgaattgt ccagtgccaa taatttttca
      721 ggcctacggt gaagtgaagc gcgagaagag ccacgctgaa acctgccgtc atccccgcga
      781 gcagccagaa atagccggcc acgcgccgct tgcgccagat gccgtgcaca atgaaaagca
      841 agatgaccca tttgacgccc gccacaaagg cgaggccagt gttctgcttc acaaaaatcg
      901 gaacgacaaa aacaattccc gcggaaaaag tgggccaggc cggaaagttt ttgctctcca
      961 atttaaacaa gaaaaacacg cacaacaata tgacgaacgt gcaatcggga tcgtaaaaag
     1021 gatgcgggaa aaccgagtaa atgcccagga cggtgatcgg cagcgtcagc agaaacgcga
     1081 taagacgaaa tttaactgtc ggttcgcgca agacccgaag cagaattcgc cacgtcacga
     1141 cggtgccgag gccgccaatc accgcacaat aaagaacgtg atggaaaaac acgcggcccg
     1201 taagcttaat cagcagcgcc tgcaccagaa acgttcccgg cgcgtaggga aacggaaaat
     1261 cgcggtacgg caactgcccc aatgaaatgc ggtaggaatt ttccaaaatg taactgaggt
     1321 cccacaaaac agcgaggcgc gaattttgcc agagcacgac tgccgcggtg cttaagaata
     1381 aaaatgccgc tgcagcccag tcgcatcctc gccatcgctc gtcgatcatg acttcagtag
     1441 gataggcttc agcctgtctg cttttgaatt tttcgcgtca aatcaaaatt caacccgcga
     1501 cgatcgcgac ttgttccgtc attccgcgcg acgcgagcaa tccctcttga ttttccagcg
     1561 ggaagcaaag aaggagagat tcctcgcctc ggactgacag cgccatccag gtcgttcgca
     1621 atgtacgatg gaagatcact ttttcaggaa cgtgccttcc tgcaattcct ggaaagcttt
     1681 ttgcagctgc gattgcgtgt tcatcacaat gggaccgtac catgccaccg gctcctcgag
     1741 cggctttccg gaaacgagca ggaagcgcac gccttcgtcg ccagcctgca cggcaacttc
     1801 atcgccctga tcgaaaagga cgagcgagcg gttgtccgcg gacgttggcg gcgtggtgtc
     1861 cgcccagccg acgccctccg tgggtacggc aagtggttcg gaggcgttgc agaatttccc
     1921 ggagcccgcg aagacgtagg cgaaggcgtg ccgcgtggtc tccaccggca atgtttttcg
     1981 ttggccgggc ggcacggaca cgtccagata aatgggatcg gcggcgacgc cgtcgaccgg
     2041 gccggacttt ccccagaatt ttccgcagac gagacgcacg tgggtgccat cgtcgtcagt
     2101 aatgttcgga atatctttgg cttcgacgcc ctggtagcga ggcgccgtca tttttagcga
     2161 gcttgggaga ttcgcccaca actggaagcc gtgcatgcgg cctgtggcat cgcccttggg
     2221 catttcctga tgaatgattc cgcttccagc cgtcatccat tgcacgtcgc caggagcgat
     2281 agagccctga ttgcccaggc tatcgccgtg atcaacggtt ccggcgagca cgtaggtgat
     2341 ggtttcgatt ccgcggtgcg ggtgccacgg gaatccggca agataatctt ccggcacatc
     2401 gttgcggaaa tcatccagca gcaaaaacgg atcgtattcc gatgtatttc cgaaaccaaa
     2461 agcgcggcgc agatgcacgc ctgcgccttc acgcgtgggc ttggatttaa cgagtcgctt
     2521 cacggggcga atggacatag ctggcctcct gcaaaggcca gtctacccca tcgcagtaaa
     2581 ggaaaacaat ttcggcctaa ttacctgagg ctgtcctgaa gccgcttgga gctttttgcg
     2641 agttgctttt ttaaagcttc aacttgcttt gtaacgtcgt cgagttcgcg ccgcgcgatt
     2701 ttaatcgcgt ccgaagtttt tctagcggcc ttatgcgcgg ttccgtccac cttacccacg
     2761 accgaaccga tttttacagc ggcgtccttt agcttgcttt tgctggccat attcgtgcct
     2821 cctgagaacc aacgatggca gatttcctgc gtcgatggca atacattatc aactgaaaaa
     2881 tggctacacc catgcgccga gcgaagtttg ccgagacgcg ctgccagacc gcatccgttt
     2941 agacctgcaa ttcaacctta cggccttaca gtagagcaag gtgggaaata ccgcttttgc
     3001 cttatgggaa gccattgcca ttacaggaga tgattccggt cggtcggttc agcctgcacg
     3061 gctggccgca tggtctaaat ttgcggaggt tccttatgtc ttttatcgct tggattatcc
     3121 tggggctgat tgctggattc gtcggcagca agctggtcaa ccatcaggga gaaggaatta
     3181 tgctggacat cgttctcggc gtcgttggtg cgatcgtggg aggttggctg ttcacgaaat
     3241 ttggcgcgca aggcgtgacg ggactgaatc tgtatagcat cgtcgtcgct gtagtgggag
     3301 cggtggtttt tctagttgct tatcacgcca tacgacgcgc ggtatagcgt ttccgcgttc
     3361 tgtagccgac aatctggcat atctaaaacg aaaattccgc acgactcgag tcgcgggtga
     3421 tttcctgtgt tcacacaaac gcctcaggag gaactcatgg gtatattcga caagatgttt
     3481 ggaagtgagg tcaaggctgc gcttgcagaa ccaaacgcac agcaacgctt cgatcaactc
     3541 aagcagaaat atcaatccgt tctgaacctg attcagcagg aaaatgttca gctcagcaac
     3601 cttcacgtgc aggccgataa actatttatt cgcggaacag ctccgagcga agcggtgaaa
     3661 aacaagatct gggacgaaat caagctggtg aatcccaaat ctgatgacat catcgctaat
     3721 ctaacggtgc gcccgcaggc cgcgcaagcc gctgctgcgg gcgcaacgtc ccaaaccggg
     3781 gctggcccgc acatcgagag cgggcaggac caaacctaca tggtgcaacc gggagacagc
     3841 ctctccagaa ttgcggacag attttacggc tccgcgcacg agtacatgaa gatttattac
     3901 gccaacaagg accagatttc aaatcccgat cagattcgcg cgggaatgaa attgaatatt
     3961 cccgtaaaga acgggtaact ggaagcgcga agggggcgtg ctgctttgtt ctgtatctcg
     4021 aacgaagtga cgaatctctc ctcgatttag gtaaaagaga ataaaaggag agatcactcg
     4081 ccccgcgcgg aatgacagga ctttgggtct cccgtagccg gtaaagcctc catgtcgtgg
     4141 tggactaatg taggggctgc ccagaggtta cttcccaaac actgctcccg cccactcctt
     4201 cgcgcgcgtt tcccctcagt ttggttccaa taaatcgtgc accgctttca ctagtc
//
LOCUS       DASZMB010000004         8156 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1928366, whole genome shotgun sequence.
ACCESSION   DASZMB010000004 DASZMB010000000
VERSION     DASZMB010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1928366"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="VG272_00055"
     CDS             <1..1213
                     /locus_tag="VG272_00055"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWF12099.1"
                     /translation="TFTASEVSKYGIFRAGLWYEWATTNRYQIPSDPLNRVDAILPNF
                     HERFYASSYQPFAEYEYHVTQRLTVTAGFKYSYFNQNLTQFQDNGKIVGCLGGILSPG
                     TKATINCIGGVPSVHHSAGYSSYLPSVDANYHLMTNWSVYAQYGMGTIIPPSGVFDVT
                     GGNVVSTPKPTSVNTYQGGTVLKLKQVTLNADVYYSKFQNAYTAIPDSNNASAVDYVT
                     GGESSTKGFEGELNVYITHGLSFYANGTAGVAKYISPTLPNSKGVPVANPDFDLWVAN
                     TPSNTEAFGLTYQQKHFELGIFDKRIGPMWNDLSLASGGVANQVIPINPFSTTNVYFN
                     YTLKNGSRWDATKFRLSVNNVFNNRGIVGDQQAFNATPTYNAGPADLLTLLPGRSITL
                     TISPGYSPKAR"
     gene            1597..2541
                     /locus_tag="VG272_00060"
     CDS             1597..2541
                     /locus_tag="VG272_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12100.1"
                     /translation="MIQRTHIAALGICVAAIGCWTSAAYAQAACCSQPSTVSANAAPS
                     PDQIHSLIVRAIENQHRDDEALQQFERVERRVARSAENAPIIEDVTERILPSTSGNIK
                     LKMADRGLPVAPEAYRSELQTALDIFGLTIHPNDHYREELAKYERRRHDRSDLVDTAM
                     KAFRITWAGRETRADSSGTHTFVKLLLDPNPDYEPINRFAASFQHVHAVLWVDEAQAQ
                     FARLEGEVSTDIPFAGGLAGKVYRGGHVGMEQQEIEPGVWLPSQYTYNVDGRKFLFAF
                     GIHERTEIARYRRIGPPSQAIEVVRNELNNLVAVKPSH"
     gene            2591..3880
                     /locus_tag="VG272_00065"
     CDS             2591..3880
                     /locus_tag="VG272_00065"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HWF12101.1"
                     /translation="MRRVCLNIALAICAAGIVCVQSRAQGPKVARQKPGGLEGQVVTS
                     KGAPVPGAQILWQVADGETPHVLHSDSHGQFHIEQLHTGVYDLRASANGTWSEWEHNV
                     LVRPGSNTNVTLRLAFKPAPPAVGLELKGTMRVWDAPVSGSVPRDSATDASGNAWFTL
                     QETGHIARFSPGTGEWKLFKIPTADSGPYGLVFDAQGDIWFTENSAGKIGHLDPKNGT
                     ISEFTPPTSKDPHSVAMGADGGIWFTSQNSNTIGRLDPFTGKIAEFGVPTQDAHPYGI
                     VLAGDQALWFCELSGGKLGRMDPATGTMMEFTPADSDVKPRELVAVGGAIYFTDFGGG
                     RLGRITLADKTFKFWDSPSGIHSAPDGIAADTAGKIWYEESGRNANQLVRFDPTVQVF
                     RTYPMPAANSQATGMARDSKGRVWLPLSGANKVAILE"
     gene            4376..5806
                     /locus_tag="VG272_00070"
     CDS             4376..5806
                     /locus_tag="VG272_00070"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HWF12102.1"
                     /translation="MRLRKARVAFSFMCIVGLRLSGPPFARAQTVSADQAPDQSASAP
                     PDDSTNNATTTIFPHFESDRVWLSGQANLITQWHPAFRSPYQGPNSLSPEAQDASSRV
                     LTLLSGFRFNNTSEILLDVQETGGHGIGEALGLAGFTNLDVVRNPTISKAPYVARLMW
                     HQIIPLGKKKASSERTPFSLFKELPVRRLEIRFGKMSVADFFDVNMYGSDSNFQFMNW
                     TLDNNGAYDYAADTRGYTYAAMVEYDDRRWSLRFAEALMPKVANDLNLDADLARARSE
                     NVELELHHSFLPHGESVVRLLGYANHANMGIYSVAVANFLAGDTPRPEITAHPFQTTT
                     KYGFGVNVEQPFTDWLGLFSRWGWNEGQHESYAYTEVDETFQIGARAEGTPWKRKLDR
                     AGLAFISNGISREHQQYLKLGGIGFLLGDGNLNYGRENILEAYYTAHVWRGIFASFDV
                     QHINNPGYNRDRGPLVAPALRLHVEF"
     gene            complement(5893..6525)
                     /locus_tag="VG272_00075"
     CDS             complement(5893..6525)
                     /locus_tag="VG272_00075"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HWF12103.1"
                     /translation="MPRRQIFQATSIQNPGSYFFRRLRSRDTNDDELLTQPPNLVAQE
                     VFNDGLISPSSKKSKTSARTRFTPWIWRTERRKMYLRKSATGFIDEESMPPFSGGELM
                     YCNRCSAEIADTANFCPACGSRQQSRTSHKQLMLSIRDKKIAGVCGGIAEYLDVDATI
                     VRLLWVALSIVPGGFIGGIVAYVLAWAIIPKTPAFVPAPVDTSQPSVKTS"
     gene            6876..7175
                     /locus_tag="VG272_00080"
     CDS             6876..7175
                     /locus_tag="VG272_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12104.1"
                     /translation="MTTETAPQTFRGVVCLHCKAVIPVPSIVGGDEASSEGNEVSSRK
                     SQVFNLRCPVCHKEKPYRTKEIVDFEGTPQTVPTLDPPVPLRWYAQDGMTRAAKA"
     gene            7417..>8156
                     /locus_tag="VG272_00085"
     CDS             7417..>8156
                     /locus_tag="VG272_00085"
                     /inference="COORDINATES: protein motif:HMM:NF018249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside permease"
                     /protein_id="HWF12105.1"
                     /translation="MTLGFLACPLGAQKPREKPRTDPPIKVKVVVVSMFEVGEDTGDI
                     PGEYQLWVEREHLEQIFPLPAGYHHVRMNKDGVLGLLTGVATAKAAASVMALGLDPRF
                     DLSKAYWIVAGIGGGDPADVSLGSAVWANHVIDGDIGYEIDAREIPADWPTGFVPLRK
                     ATPYEQPVKSQLDGEAYTLNQDFVNWAYQLTKNVSLADSDKMRNTRMLYVGFPNAMKL
                     PFVVRGDTMSGGTFWHGKKMDEWANAWT"
BASE COUNT         1873 a         2321 c         2165 g         1797 t
ORIGIN      
        1 gacctttacg gctagcgaag tgtccaaata tggcattttc cgcgcgggac tttggtacga
       61 gtgggccact acgaatcgtt atcaaattcc gtcggaccct ctcaatcgcg tcgatgccat
      121 cctcccaaat ttccatgagc gtttttacgc cagttcgtat cagccttttg ccgaatacga
      181 ataccacgtc acgcagcgcc taaccgtcac tgccggcttc aagtactcct atttcaatca
      241 gaacttgacg cagtttcagg acaacggaaa aatcgtcggc tgcctgggcg gcattttgtc
      301 ccccggaacg aaggcgacta ttaactgtat tggcggagtt ccttccgtcc accacagcgc
      361 cggatacagc tcgtacctgc cttcggtaga tgcgaattat cacctcatga cgaattggtc
      421 ggtgtatgcc cagtacggca tgggcacgat cattccgccc agcggagtgt tcgatgtaac
      481 gggcggaaat gttgtttcca cgccaaaacc gacgagcgtg aacacctatc agggcggaac
      541 ggttctgaag ctcaaacaag tgacgttgaa cgccgatgtc tattacagta aattccagaa
      601 tgcctacacc gcgattcccg attcaaataa cgcgagcgcc gtcgattatg tgaccggagg
      661 tgaatcgtca acgaaaggct tcgaaggcga actgaacgtt tacatcactc acggcctgag
      721 cttctacgcc aacggtacgg ctggcgtcgc caagtacatc agcccgacat tgccgaacag
      781 caaaggcgtc ccggtcgcaa atcccgattt tgatctttgg gtcgcgaata ctccctccaa
      841 cacggaagca ttcggcttga cgtaccaaca gaaacacttc gagctgggaa tttttgacaa
      901 gcgcatcggc cccatgtgga atgaccttag tcttgctagc ggcggagtcg ccaaccaggt
      961 gattcccatc aacccgttca gcaccacgaa cgtttatttc aactacacgc tcaagaacgg
     1021 gtcccggtgg gacgccacca agttccggct cagcgtcaac aacgtgttca acaatcgcgg
     1081 aattgtgggc gatcaacaag cctttaacgc cacgccaacc tacaacgccg gcccagccga
     1141 tctgcttacc ttgcttccgg gccgcagcat cacgctgacc atcagccccg gttactcgcc
     1201 gaaggcgcgt taagcaagag gctagcggca taggccgcgg aaaaaccaac cctccgtgat
     1261 tccgagcgaa gcgaggaatc tctccttctt ttgttttctt atattaaatc gaagagagat
     1321 ttttcgctgc gctcgaatga cagaaaattg tactttttcc gcggccgggg cagtcgccgc
     1381 catttgctga ttgaccgtca atagaggttt acatgtattg agcatttaac gcgggcgcct
     1441 atagttgcat aggcgcccgc tttcttttca cttcgcaact ttttatggga gggtgccttt
     1501 tgagaaacgc gtcggtacaa ccgccgtatg cgccttagaa tctgagtagc agtggcagaa
     1561 ggggggccag ttctggcaga cgcagcataa tggctcatga ttcagcgaac ccatatcgca
     1621 gctctcggaa tatgcgtcgc cgctataggc tgttggacga gtgcggccta cgcgcaggca
     1681 gcctgttgca gccagccgtc caccgtctct gctaatgccg ccccaagccc cgatcagatt
     1741 cactcgctga tcgttcgagc catcgaaaat cagcatcggg acgacgaagc gctgcaacag
     1801 ttcgaacgcg tggagcgccg cgttgcccgc agcgccgaaa acgcgccaat catcgaggac
     1861 gttaccgagc gcatccttcc atccacgtcc ggcaatatca aattaaaaat ggcggaccgc
     1921 ggtctaccgg tcgcgcccga agcgtatcgc agcgagttac agaccgcatt ggacatcttc
     1981 ggactcacca ttcaccctaa cgatcattac agggaagaac tggcgaagta cgagcgccga
     2041 cgccacgacc gctccgacct cgtggacacc gccatgaaag catttcgaat cacgtgggcg
     2101 ggccgcgaaa cgcgtgcgga ttccagcggc acacacacct tcgtaaaatt gttgctggat
     2161 ccgaatccgg actacgagcc catcaatcgt ttcgccgcga gttttcagca cgttcacgcg
     2221 gttctatggg tcgacgaagc tcaagcgcaa tttgcgcgcc tcgaaggcga ggtctctaca
     2281 gacattccgt ttgccggcgg cctggccggg aaagtgtatc gaggcggcca tgttggcatg
     2341 gagcagcaag aaatcgagcc gggagtctgg ctcccgagcc aatacaccta caacgtcgat
     2401 gggcgtaaat tcctgttcgc ctttggcatt cacgaacgca ccgaaattgc ccgctatcgc
     2461 cgcattggtc ccccgtccca agccatcgaa gtcgttcgta atgaattgaa caatctggtc
     2521 gccgtaaaac cgtcgcatta accgaagcaa ggtaaaatcg taggggcaag tcatcccgga
     2581 gggtctccct gtgcggcgcg tgtgtctgaa catcgccctg gctatttgcg cggcgggaat
     2641 cgtgtgcgtc caatcgcggg cgcaggggcc aaaggtcgca aggcaaaaac cgggcggcct
     2701 ggaaggccag gtggtcacat caaagggcgc ccctgtgccc ggcgcgcaga tactgtggca
     2761 ggtggccgac ggagaaactc cgcacgtgtt gcacagtgac tcgcacgggc aattccatat
     2821 cgaacagttg cacacgggcg tttacgattt gcgagcctct gccaacggca cctggtcaga
     2881 atgggaacac aatgtcctag tccgcccagg ctcgaatacg aatgtcactt tgcggcttgc
     2941 attcaaacca gcccctccag cggttggttt ggaactgaag ggcacgatgc gcgtttggga
     3001 cgcgcccgtt tccggctcgg tcccccgcga ctccgcaacg gatgccagcg ggaacgcctg
     3061 gttcacattg caggaaacgg ggcacatcgc gcgcttcagt cccggcacgg gcgaatggaa
     3121 actcttcaaa attcctacgg cagattccgg tccgtatggc ttggtatttg acgcacaggg
     3181 cgacatctgg tttacggaaa atagcgctgg aaaaattggc catctcgatc caaaaaacgg
     3241 aactatttcc gaatttactc cgcccacatc caaggatcct cactcggtgg cgatgggggc
     3301 ggatggcggc atatggttca cctcgcaaaa ttcgaacacg atcggccggc tggatccctt
     3361 cacggggaaa atcgccgaat ttggcgtgcc cacgcaggac gcccatccct acggcatcgt
     3421 gctcgccgga gaccaggcac tgtggttttg tgagttgtca ggcggcaagc tgggccgcat
     3481 ggatcccgcg accggaacca tgatggaatt tacgcccgcc gattccgacg taaaacctcg
     3541 cgaactcgtt gccgtgggcg gcgcaattta ctttaccgat ttcggcggcg gccgcctcgg
     3601 acgcattact ctggccgaca agactttcaa attttgggac agcccttccg gaattcactc
     3661 cgctccggat gggatcgcgg cggatacagc cggcaaaatc tggtacgaag agagcggaag
     3721 gaacgccaat cagctcgtgc gcttcgatcc tacggtccag gtgtttagaa catatcccat
     3781 gcccgcggca aactcccaag ccacaggcat ggcccgcgac agcaaaggcc gcgtatggct
     3841 gccactttcc ggtgcgaata aggtcgccat ccttgaataa aatttcgaca taagcatctt
     3901 tattcctgcc agttacggga tacctggatc gttcggtacg aatagcctgg ggcacttcac
     3961 gttgcacgta atctacctag tactaaaacc cgggtcccaa aaaatttgaa tccgtaagcc
     4021 cattgagcga accgcatcac tcctacaaca tggttccact gtgggttggg agtcgcgctg
     4081 cggggacgac ggccccgaga gatcgggtct gtaagcgctc tacccaaccc gccaaaattt
     4141 ctggacacaa cgggaacact attaaagctt cttccaaatc aaatccgccc gagcaatcac
     4201 aaagcaatcc tgccaggcaa attcttctct tgagggatag ctttaatcct ttgttcatct
     4261 ccaaattgca gctcgtcgtg atttaatagg ggattcagtt tgccggtttc tttggcggat
     4321 ggtactccag ccaaatcgtc aatctgacaa atgacggcag gcgcagcgag agaacatgcg
     4381 gctacgtaaa gccagagtcg ctttttcatt catgtgtatt gtggggctgc gactctccgg
     4441 tccgccattc gcgcgtgctc agaccgtttc ggccgatcaa gcgcccgatc aatccgcctc
     4501 cgcgccgccg gacgattcca ctaacaacgc aaccaccacg atcttcccgc attttgagtc
     4561 tgatcgcgtc tggctttcgg gacaggcaaa tttaatcacc cagtggcacc cggcgttccg
     4621 gtctccctat caaggaccga acagcctgtc gccagaggcg caggacgcct cttcccgcgt
     4681 cctaactttg ctgagcggtt tccggttcaa taacacttct gaaattctgc tcgacgtgca
     4741 ggaaaccggc ggccacggaa ttggtgaagc gctcggcctc gcgggtttta caaatctaga
     4801 tgtagtccgg aatccaacca tcagcaaagc tccgtatgta gcgcgactga tgtggcatca
     4861 gattattccc cttggtaaga agaaagcgtc ttcggagaga actccttttt ccctattcaa
     4921 agaattgccc gtgcggcggc tggaaatccg ctttggaaaa atgagtgtag cggatttttt
     4981 tgacgtgaac atgtacggct cggacagcaa ttttcaattc atgaactgga ctctggacaa
     5041 caacggcgcc tacgactacg cagcagatac gcgcggttac acgtatgcgg ccatggtgga
     5101 gtatgacgac cggcgctggt ccctgcgatt tgccgaagcc ctgatgccaa aggtcgccaa
     5161 cgatttgaat cttgacgcgg atctcgcccg agcgcgctcc gaaaatgtgg agcttgagct
     5221 gcaccattca tttctgccgc atggagaatc cgtcgttcgc ctgctcggct acgccaatca
     5281 tgccaacatg ggaatttaca gtgtggccgt tgcaaatttt ttggctggcg atacgccacg
     5341 gcctgaaatc accgcgcacc catttcagac caccacaaaa tacggctttg gcgtgaacgt
     5401 cgagcagccg ttcacggact ggctcggttt attttctcgc tggggctgga atgaagggca
     5461 gcacgagtcc tacgcctaca cggaagtaga cgagacgttt cagatcgggg cacgtgcgga
     5521 aggaactccg tggaaaagaa aactggatcg cgcgggcctc gccttcattt ctaatggaat
     5581 ttcgcgcgaa catcagcaat acctgaagct cggaggaatt ggatttttgc taggtgacgg
     5641 aaatttaaat tacggccgcg aaaacattct ggaggcctac tacaccgcgc atgtgtggcg
     5701 cggcatcttc gcttcgttcg acgtgcagca catcaacaac cccggttaca accgtgaccg
     5761 cggcccgctc gtcgcgcctg cacttcgcct acatgtcgag ttttgatttt tgtggcatag
     5821 gcttcagcct gtggggtttt gtcctggcgc actccaaacc ccacaggctg aagcctattc
     5881 cacgtagaac cattaactcg tcttcaccga cggctgcgac gtatccaccg gcgccgggac
     5941 aaacgccgga gttttaggaa tgatagccca cgctagaacg tacgccacaa ttcctccgat
     6001 gaagccgcct ggtacgatcg acagggcgac ccagagcagc cgtacgatcg tagcgtccac
     6061 gtccaaatat tccgcgatgc ctccgcaaac tccggcgatt tttttgtcgc ggatcgaaag
     6121 catcagctgc ttgtgagatg ttctcgattg ctgccgcgac ccgcaagcgg ggcaaaaatt
     6181 ggcagtgtcg gcgatttccg cgctgcaacg attgcaatac ataagctcac ctcctgaaaa
     6241 cggcggcatg gattcctcat cgatgaaccc agtcgccgat ttccgcaggt acattttacg
     6301 ccgctcggtc cgccaaatcc acggcgtgaa tcgcgtgcgc gcgctggttt tggatttctt
     6361 cgaacttgga ctaatgagcc cgtcgttgaa aacctcttga gcgaccagat ttggaggctg
     6421 cgtgagaagt tcatcgtcat tggtgtcgcg gcttcgcagc ctgcggaaaa agtaactgcc
     6481 aggattttga atcgaagtcg cctgaaagat ttggcgacgg ggcaaaacag cctaaattta
     6541 ttcggcagat acacttcgcg gcgggctctt acggctattt ttgaacacgt ttggcggctt
     6601 cgcggcgccc tcgggaaaga ttttttgtag gatcggccta atcgcccgat gctcgaaagc
     6661 tgtggaaatg ccgttctgga acatcaacga gccgcgggaa cgggaatcac tcacactgct
     6721 cataataaag gatttagtat gctaccggag gtattaattc gtcgcttttt catttccaca
     6781 gttttcggtt tttatccgac tggacgtgcc ttgacccatg gagtagcatt gacttgctcc
     6841 caataaacgg aattctacga tacgaggttt tacttatgac gactgaaacc gcaccccaaa
     6901 cttttcgagg cgttgtatgc ctgcattgca aggccgttat ccccgttcca tcgattgttg
     6961 gaggcgacga agcctccagt gagggaaacg aagtttcgtc cagaaaatcc caggtattca
     7021 acctgcgctg cccggtgtgc cacaaagaaa agccctatcg cacgaaggaa attgtcgatt
     7081 tcgaaggcac cccgcaaacc gttccgactc tcgatccacc ggtgccgctg cgatggtatg
     7141 cccaggacgg aatgacgcga gcggccaagg cgtagccgca agttagtccg cttttttctc
     7201 aaaaatccgg cagttccttt ttacttgcca tattcccttg cccagccctt ccacgcttta
     7261 atctatatgc catccatcac gaattaaaat tgaacaggaa tctctcctgg ccgcgctctg
     7321 atgtaaactc aaacaccctg ctgcagggaa tagggcatcc gcaaccgccg gagagaattt
     7381 ttggattttt cttttaaatt tttcttaggg agtgttgtga ctctgggctt tctggcctgc
     7441 cccctcgggg cgcaaaagcc gcgggagaaa ccgcgcaccg atcctccaat caaagtaaaa
     7501 gtcgtcgtcg tgtccatgtt tgaagtggga gaagataccg gcgacattcc cggggaatat
     7561 caactgtggg tcgaacgcga gcatctggag cagattttcc cgctgcccgc cggctaccat
     7621 cacgtgcgca tgaataagga tggcgtgctc ggcctgctca ccggcgtagc cactgccaag
     7681 gccgcagctt cggtgatggc gctggggctc gacccgcgct tcgatctttc caaggcgtat
     7741 tggattgtcg ccggaattgg gggcggcgat cccgccgatg tgtcgctggg ttctgcagtg
     7801 tgggcgaatc acgtgattga cggcgacatt ggatacgaaa tcgacgcgcg tgaaattccc
     7861 gcggactggc cgacaggttt tgttccgctg cgcaaggcca ccccctacga acagcccgtg
     7921 aagtcacaac ttgatggaga agcctatacg ctcaaccagg acttcgttaa ttgggcgtat
     7981 cagttaacaa aaaatgtctc tctcgcggac agcgacaaaa tgcgcaacac gcgaatgctt
     8041 tatgtgggat ttcctaacgc aatgaagctt ccctttgtgg tacgcggcga tacgatgtcc
     8101 ggcggcacgt tctggcacgg caaaaaaatg gacgaatggg cgaacgcgtg gacgaa
//
LOCUS       DASZMB010000005         3974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2203627, whole genome shotgun sequence.
ACCESSION   DASZMB010000005 DASZMB010000000
VERSION     DASZMB010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2203627"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(322..397)
                     /locus_tag="VG272_00090"
     tRNA            complement(322..397)
                     /locus_tag="VG272_00090"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Glu,seq:ttc)
     gene            complement(593..811)
                     /gene="iscX"
                     /locus_tag="VG272_00095"
     CDS             complement(593..811)
                     /gene="iscX"
                     /locus_tag="VG272_00095"
                     /inference="COORDINATES: protein motif:HMM:NF016284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HWF12106.1"
                     /translation="MPATFDWNDAEEIGIQLADKYPDADPLNVRYTDLHKWITSLEGF
                     TGDPKQSNESKLEAIQMAWLEEHNDRKA"
     gene            complement(1074..1484)
                     /locus_tag="VG272_00100"
     CDS             complement(1074..1484)
                     /locus_tag="VG272_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWF12107.1"
                     /translation="MGGSNPYIEKTKFTPAQKKFKVTFVREGKTVEVDPERVPYAREG
                     LPGSLLDIATGFHMGLDHACGGVCACSTCHVIVHEGIESCNEATDDELDQLDEAAGVT
                     AKSRLGCQCVPDGSRDLVVEIPEWNKNFAKEAEH"
     gene            complement(1580..3427)
                     /gene="dnaK"
                     /locus_tag="VG272_00105"
     CDS             complement(1580..3427)
                     /gene="dnaK"
                     /locus_tag="VG272_00105"
                     /inference="COORDINATES: protein motif:HMM:NF003520.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HWF12108.1"
                     /translation="MGKTVGIDLGTTYSLIAYQDKREGRPKCIPGPYGTPLCPSVVSV
                     DADGSIVVGQAAQRRLLTQADRTIYSVKRLMGRGPADVQDELKLFPFRIDPVSKNVIR
                     VRLGDRVFTPPEISAFILRELKMWAEEFFQDSVDRAVITVPAYFNDAQRQATRDAGRL
                     AGLEVLRLVNEPTAAALAYGLHEQKRGKVAVYDFGGGTFDISILKLISTTDGDIYQVL
                     STNGDTHLGGDDIDNSLHSLARAEILSQGVDIASHPELIQELRKALIAAKHELSDADK
                     TTLKFPLPDGRAYFREISRAEYDRLIRPIVDRTMGPVRQALSDAKLSPADIEEVVLVG
                     GSTRVPLVRQTVEQFFGRKPHAELNPDEVVALGAAVQADILEGGVNDMLLLDVTPLSL
                     GIETMGGVVAKIIPRNSTVPASAQELFTTGVENQTGIDIHVLQGERELAKDCRSLSRF
                     QLKVPPGPAGLARIEVKFLIDASGILQVAAKDLRAGTEHSIEVQPTYGLDDAEVERML
                     EESIEFAEQDFSQRQLIEARNEAESILTLTEKALARELSEAPGELPEAERIQIQSAIS
                     ALREALQGTDYKSIRAFGDVLNQASTPLAERMMNRALGEALEGKKLGDV"
BASE COUNT          902 a         1097 c         1048 g          927 t
ORIGIN      
        1 tataacttta tcgttaaatc gcatgctcca cttctccaca ttaaccctat ttctgaacat
       61 acaactgacg attcaagaaa gcaaatgccg ggcggttgat gacggctgaa gaacctgcga
      121 agtcggcctg ttcggcccga aatacgcggt tcaccaagaa cgttctccgt gagcgggacg
      181 gacaggccgc tacgaaaccg gtttacgtag agccgttcgc tttttgaacg ggcggtcttc
      241 ctgcatgcgc aattcaaagt aaaaagaagg ccatgaattt agttgagatt aaagagaaga
      301 gggctgctat tacaacaaat ttggtagccc ggacgggggt cgaacccgta atcttcgcct
      361 tgaaagggcg acgagttaac cattactcta ccgggccaca aagcgttacg ccgaggccag
      421 ccaagcgctt tggaattcta gcacattcgc gcgatttgca tcaggacacg ggccgaagat
      481 tatttgtaga aattggggga gcaaagtcta tgcctaaaat ggaatctgac ccgtggattt
      541 gtaaatggtt ttcaccagtc accaatcacg agtcaccagt cacatgtgtt tctcacgcct
      601 tgcggtcgtt atgctcctct agccaagcca tttgaatcgc ctcgagcttg ctctcgttcg
      661 attgcttggg atcgcctgtg aatccttcga ggcttgtgat ccacttgtgc aggtccgtgt
      721 agcgcacgtt gagtggatcg gcgtccgggt atttatcggc cagctggatt cctatttctt
      781 cggcgtcatt ccaatcaaac gttgcgggca tggttccgtc tcccttgtag ctcgcctcgc
      841 ttgtcccgag tgaaaacgag ggatcagagg cgagggttta gatgaaagtg tgcgccaaat
      901 ttcctgctca gcgcgcatac tcgagttaat gacggctgcc tctacctcta catcagtgcc
      961 cccatttcag gctttgaatg agacatcgtc taaaaccccg cctctaaaga gacgggctac
     1021 agcagtcggc caaaactttc gccacgtcgt aaaacatacg aatgcgctgc gtcctaatgt
     1081 tccgcttcct ttgcgaaatt cttgttccac tccggaattt cgaccaccaa atcacgcgag
     1141 ccatccggga cgcactggca accgaggcgt gatttcgcgg ttacaccggc agcttcgtcc
     1201 agttgatcga gttcatcatc ggttgcttcg ttacatgact cgatgccctc atggacgatc
     1261 acgtgacacg tggagcaggc gcagactccg ccgcaggcat ggtcaaggcc catgtgaaat
     1321 ccggtggcga tatccaggag gcttcccggc aagccttcgc gcgcgtaggg aactctttcc
     1381 ggatcgacct ccaccgtttt gccctcgcgt acaaacgtca ccttgaattt cttttgcgcg
     1441 ggcgtgaact tggttttctc gatataggga ttactgccgc ccatagtgtt ctccttctcc
     1501 gttacttctg gaactgttgc aatcgtttca ggctggcctc gcttttctca actaaaccca
     1561 cagtctgaag cccgcgctac taaacatcgc ctagtttttt gccttcaaga gcttctccca
     1621 gtgcgcggtt catcatgcgt tcagcgagtg gcgtggaagc ctgattcaga acgtcgccaa
     1681 atgcgcgaat gcttttataa tcggtgcctt ggagcgcttc gcgcagcgct gaaatagcgc
     1741 tctggatctg gatgcgttcc gcctcgggca attcacccgg agcttcggaa agttcccggg
     1801 ccagagcttt ctccgtaaga gtaagtatgc tttcggcttc gttgcgcgct tcgatcaact
     1861 gacgctgcga aaaatcctgc tccgcaaact cgatggattc ttcgagcatg cgctcgactt
     1921 cggcgtcgtc caggccatag gtgggctgca cttcgatgga gtgctcagtg ccagcgcgta
     1981 aatcttttgc cgctacctgc aaaatgccgc tggcgtcgat caggaatttc acttcgatgc
     2041 gcgcgagacc cgcgggaccc ggcggaactt ttaattgaaa tcgcgaaagg gagcggcaat
     2101 cttttgccaa ctcgcgctcg ccttgcagga cgtggatgtc gatgccagtc tgattctcga
     2161 cgcccgtggt aaataattct tgcgcgctgg cgggcacggt ggaattcctt ggaataattt
     2221 tcgcgactac gccgcccatg gtttcgattc caagggatag aggagtcacg tcgagcagca
     2281 gcatgtcatt cacgccgcct tcgagaatgt ccgcctgaac ggcggcgccg agtgcgacca
     2341 cttcgtccgg attgagctcc gcgtgcggct tgcggccgaa aaattgctcc accgtctgcc
     2401 ggacgagcgg gacacgtgtc gatccaccga cgagcaccac ttcttcgata tcggcgggag
     2461 aaagtttcgc gtcggacagc gcctggcgta cagggcccat cgtgcggtca acaatagggc
     2521 gaatcagacg atcgtattcc gcgcgcgaaa tttcgcggaa ataggcacga ccatcaggca
     2581 aagggaattt taaggttgtt ttgtccgcgt ccgaaagttc gtgcttggcg gcgatgagag
     2641 ccttccgcaa ttcctggatc aattccggat gcgacgcaat gtcgacgcct tgcgaaagaa
     2701 tttccgcacg cgccaagctg tgcagcgagt tatcgatatc gtcgcccccg aggtgcgtgt
     2761 cgccgttggt cgagagcact tgatagatat cgccatccgt ggtggaaatt aatttcagga
     2821 ttgaaatgtc gaatgtgccg ccgccgaaat cgtagacggc cactttgccg cgcttctgtt
     2881 cgtgcaggcc gtaggcgagc gcagctgcgg taggctcatt cacaagccgc aggacttcaa
     2941 gcccagcgag gcggcctgcg tcgcgcgtgg cctggcgttg cgcgtcatta aaatatgcgg
     3001 gtactgtgat gaccgcgcga tcgaccgaat cctgaaaaaa ttcttcggcc cacattttca
     3061 actcgcgcag aatgaacgcc gaaatttccg gcggcgtgaa cacgcgatcg ccaaggcgca
     3121 cgcgaatcac atttttgctt acaggatcga tgcggaacgg aaacagcttc agttcgtcct
     3181 gcacgtcggc aggaccgcgg cccattaagc gcttcacgga ataaatggtg cggtccgcct
     3241 gcgtcaacaa tcggcgctgc gcggcctggc ctacgacaat cgatccatcc gcgtccacac
     3301 tgaccaccga aggacacaga ggtgtgccgt acggccccgg aatacacttc ggccggcctt
     3361 cgcgtttatc ctgatacgcg atcaggctgt aggtggtgcc gagatcgata ccgacggtct
     3421 ttcccattca gcaatgcccc cgatgagagt gactggtgat tagtgactgg tgactggtga
     3481 aaaacaacac gaacgtcggg cttggttttc acgagtcacc agtcactaga agctatctca
     3541 taaatatgaa aatcttacat accattacag cgggccgacc gacggagaag ctgaaaccca
     3601 ctaaacaaag gtttttctcg cgccagcgct tgctaactca ttcatctgga gagacctaaa
     3661 accatttatg agatagcttc tagatacata tcgaaaagca gacttctttc ccctcagcga
     3721 cgattacgtg gcaggcgcaa ccgacgtagg acgaaccact acttcgattc aggtcgggac
     3781 actgcgaaaa atcatcaccg atgattgggt ggcagttccg cctgcactca aagaagtaaa
     3841 aattgccatc ctgaagtact ctcattcaga ccaatgggca aagggaaacc ttgacactgt
     3901 tcccggcttg tttaacgaaa tatccagccc agttttaagg aaaagaatcc tttccgaaat
     3961 actgccacgc gtag
//
LOCUS       DASZMB010000006        11079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3853169, whole genome shotgun sequence.
ACCESSION   DASZMB010000006 DASZMB010000000
VERSION     DASZMB010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 11079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11079
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3853169"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3251
                     /locus_tag="VG272_00110"
     CDS             <1..3251
                     /locus_tag="VG272_00110"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HWF12109.1"
                     /translation="VPGLLRLGSPQLVRAGPVRLMYTELHARSAFSFLEGASLPEELA
                     GACAELGIPGMALLDRDGVYGAPRFFLAANKSKICAHVGAEITSEAGWRYPLLVTSQE
                     GYQNLCRLITRMKLRARKGEGHVQIAELADRTAGLICMTGGEEGPLAHALGNGGIKNG
                     IRCMQQLCELFGRTNVYVELQRHLSREEEARNQAAVEIARKLHLPLLATNGVCHAVPQ
                     RRQILDVFTCIRNHKNLGSAGRLLSLNSERHLKAPAKMKRLFSDLPEAIANTQELSSR
                     LQFTLNDLGYKFPVYPVPDGATQIHFLRKLSRDGLISRYGNVNERARLQIEHELTVIE
                     KLDLAGYFLIVWDIVRFCRDRGILVQGRGSAANSAVCYALGITAVDPVGMDLLFERFL
                     SEERGEWPDIDLDLPSGEQRERAIQYVYQRYGQLGAAMTANVITYRGRSAAREIGKVL
                     SFDVETISRLTKLVSAWEYKAANDTLANQFHDAGFDLKHPQIRKFFDLCLEVQDLPRH
                     LGQHSGGLVICQGQLDSVVPLEPATMPGRVVVQWDKDDCASMGIVKVDLLGLGMMAVL
                     EESLQIIHKQYGEEIDLGKLPANDPVVYSTLQKADTVGMFQIESRAQMSCLPRLVPKC
                     FYDIVVQVAIIRPGPIVGQMVNPYLKRRQGRETVDYLHPSLEPVLARTLGVPLFQEQL
                     LRMAMIAAGFSGGEAEELRRAFGFKRSEVRMKDIEQKLRLGMTRNHIASEIQEKIILA
                     ITSFALYGFPESHAASFALIAYASAYLKCHFLPAFTAAILNNQPMGFYQPATLVKDAQ
                     RHGLKVRPVNVTCSDWLCTLEENGEDFAMRLGMRCVTGLRREVAETIVRERAIFPFTS
                     IHDLKLRVPFIHKSELTMLAEIGALNFISGKNSIHRRDALWQVERVARRAGPLLEKIE
                     LPAMSEGEFHAEERLYGDEPSGFKTSPLKPMTSEEKLRADFNGTGMTVGPHPMSYHRK
                     KLKRQGVRCASELSSLPNGIPVRIAGAVIARQRPGTAKGFVFLSMEDETGIANAIISP
                     QTYDTYHLIVVHQQFLLIEGQLQNQENVISVKAEVIRPLPIARVESSSHDFH"
     gene            3624..4442
                     /locus_tag="VG272_00115"
     CDS             3624..4442
                     /locus_tag="VG272_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HWF12110.1"
                     /translation="MPSSVWKGYISFGLISVPVRLYAAARYSHVAFHEIHRKCGTRVH
                     QQLYCPYDQEVVTRDEIAMGYEVDKDKYVLVEPSELKSLQPPSSTAMEIIQFVKLSEV
                     DPIYFETSYFSVPEEAGQKAYALLLNAMEDKGYAAIAKVTMHQRERTVIIRSYQKGLI
                     LHTIYYPSEIRDIKGYGQPTAQNLKKQEIDLAEQFAASLVKPFRPEQFHDEYSERVQE
                     LIESKSKGKAAPKPEKSQRLAPVVDLMTALKKSLAGAKHSGGGSRSSKRLRKTA"
     gene            4442..5086
                     /locus_tag="VG272_00120"
     CDS             4442..5086
                     /locus_tag="VG272_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12111.1"
                     /translation="MKTSRAANSDSNKKLKTLAPVTKVRRTTAAAAMPAVPIEEVFSF
                     LKETRGVVSWTIADIVDALKIGEAEAKRIVQILELQGYVKQQNDSGDTAQWLTTLSGE
                     SVSGSAAPRFTSDRVKKGLAALADRIRAINRDQHAALSIPQAVAFGDFLRTRTRVQAA
                     DVGVRIESKPHAGKKQNPQHSEGDFLKQLKARSALVNVQIYKPWMSKRSHVRLV"
     gene            complement(5349..7649)
                     /locus_tag="VG272_00125"
     CDS             complement(5349..7649)
                     /locus_tag="VG272_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWF12112.1"
                     /translation="MASATISRRDFVKNAAGLVIGFSLFGQQLRAAELGEQSGFNGSP
                     LPTQLDSWLVIGSDGIITVFTSKVDLGTGVLTALSQVMAEELDVSFERIHMITGDTSQ
                     TIDQSQTSGSRTLHKAGPQLRQAAAAGKHELLKLAAVRLDVPAEKLAVDDGVVSVVDN
                     PSTNISYWKLLGGKRFDVAITATGTAAEMKVAPEISPKNAKDYKIVGTSIPRIDLPAK
                     FTGEFTYSQDVRIPGMLHGRVVRPEHSLARLVSVDENSIKDIPGIVKIVKEDHFVGIV
                     AATEWSAIRASQALKAAWSEPEAKLPASRDDLYSYLKETKPFKEQLVANRGDFDAALS
                     RSSRVVEATYHWPFHMHGMIGPSCAVADVRDGKATVWAGSQGTHRTRKAVADLLRLPE
                     NNVRIIYAEGSGCYGRLCADDVAEDAALMSRAVNKPVRVQWMRHEEHAWEPKGSAQLM
                     TVRAGVDPRGNITAWDFMDRYFPYTAGPGIPLLASTQTGLRPNGPGFPGNGYEYGTTG
                     SGDLYAFENQRINCPVIPWIQNAMSHLRTCNLRAPGAVGRTFASESFIDEVAFHAGVD
                     PVQFRLRSPRNQQRTSEVLLAAAKQAGWVERPSPAPAQTNPDKCVGRGAAMSDRDGAI
                     VAAVAEVEVEKSTGKVTVTRVTVAHDCGLIANPDGLKNQIEGNVVQGVSRSLLEEVQF
                     DENGVTNMDWSSYKTIRFEDIPEIDIVLIDRPELGFLGAGEASIIPVPAAIANAVFDA
                     TGARVRDIPLTPERVLRALQKLESRT"
     gene            complement(7651..8130)
                     /locus_tag="VG272_00130"
     CDS             complement(7651..8130)
                     /locus_tag="VG272_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWF12113.1"
                     /translation="MSRISLTVNGKARTIDVDPSTPLLYVLRNEFRLNGPKFGCGMSQ
                     CGACTVLSEGRSIRSCSVPVSSMEKRSIITLEGLGNSTHPGFLQNAFVEEQAAQCGYC
                     ANGMIMTAQALLDRNANPSVAEIKQALNANLCRCGTHVRIVRAVKRAAMERNKTGRA"
     gene            complement(8356..8853)
                     /locus_tag="VG272_00135"
     CDS             complement(8356..8853)
                     /locus_tag="VG272_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HWF12114.1"
                     /translation="MNWTRSVIALSVLLIFSVAMPGLRVHAQSTSTDADSAAIRKVID
                     DFVGAFNRHDAAGWAMPFGDDGDFTNVSGLTKHGRKEVEERFKVLFAGPLKNAHRTVT
                     VRHLRFAKPDVAFADAEWALEGSLAGDGSANPVRKGIFTFVLVKQDGYWKFADFHESE
                     FVTMK"
     gene            10356..10868
                     /locus_tag="VG272_00140"
     CDS             10356..10868
                     /locus_tag="VG272_00140"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HWF12115.1"
                     /translation="MSAIDDDDDRVFTVVIETPRGCRNKYAFDPDQRVFSLKKVLPAG
                     MAFPYDFGFVPSTEGEDGDAIDVLVLMDEPAFPGCVVRCRLIGVIEGEQKDGKNVERN
                     DRILAVEIGTHSWDDVRHVDDIGKQFAKELAKFFVHYHELTGKEYRVLAMKEPGAATK
                     CIAKGMKAAK"
     gene            complement(10861..>11079)
                     /locus_tag="VG272_00145"
     CDS             complement(10861..>11079)
                     /locus_tag="VG272_00145"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HWF12116.1"
                     /translation="EGWISITPFHMSIRLRGLPVLPGMSAIPELNFRTYVTVQNKPGV
                     YFFSLDTVSHAAVWGARMLYHLPIASPT"
BASE COUNT         2575 a         2921 c         3051 g         2532 t
ORIGIN      
        1 ctgtaccggg tttattacga ctgggttcgc cgcagctggt tcgtgcgggg ccggtacgat
       61 tgatgtacac agagcttcat gcgcgttcgg ctttcagttt tctggaggga gcttccctgc
      121 ctgaagaatt ggccggcgcg tgcgcggaac ttggcattcc gggcatggct ttgctcgacc
      181 gcgacggcgt gtacggcgct ccacgttttt tcctggccgc aaataaatcc aaaatttgcg
      241 cgcacgtcgg cgcggagatt acttccgaag cggggtggcg ctatccgctg ctcgtaacat
      301 cccaggaggg ataccaaaat ctttgccgcc tgatcaccag aatgaaattg cgtgcgcgaa
      361 aaggcgaagg ccatgtgcag atcgcggagc ttgccgatcg cacggccggc ctgatctgca
      421 tgacgggcgg cgaagagggg ccgctggcgc atgccttggg gaatggcgga atcaaaaacg
      481 gaattcgttg catgcagcaa ttgtgcgaac ttttcgggcg cacaaatgtt tatgtggagc
      541 tgcaacgtca tctttcgcgc gaagaggaag cgcgcaacca ggcggccgtt gaaattgcgc
      601 gaaagctgca tctgcccctg ctggcaacca atggcgtatg ccatgccgtg ccgcaaagac
      661 ggcaaatcct cgacgttttt acatgcatcc gaaatcacaa gaatcttggc agcgcagggc
      721 ggcttctctc tctgaattca gaacgtcatt tgaaagctcc cgcaaaaatg aagcggctgt
      781 tttccgatct tcccgaggcc attgcaaaca cgcaggaact ttcctcccgc ttgcaattca
      841 cgctgaatga tctgggatat aaattcccgg tctatcccgt tccggatggc gccacgcaaa
      901 tacatttttt gcgaaaactc agccgggacg gattgatctc ccgctatgga aacgtcaatg
      961 aacgcgcgcg gcttcaaatc gagcatgaat taaccgtcat cgagaaactt gatctggcgg
     1021 gatatttttt aattgtgtgg gacatcgttc gtttctgccg cgatcgcggc attctggtgc
     1081 aggggcgagg ttccgctgca aacagcgcgg tgtgctacgc gctgggcatc acggccgtcg
     1141 atccggtggg aatggatttg ctttttgaac gattcctttc cgaagagcgc ggagaatggc
     1201 cggacatcga tctggatttg ccgagcggag agcagcgcga acgcgccatt caatatgtat
     1261 atcaacgtta cggacaactg ggcgcggcga tgacggcaaa cgtgatcacg tatcgcggac
     1321 gttccgcggc gcgcgaaatc ggaaaagttt tatcgttcga cgtggaaacg ataagccgcc
     1381 tgaccaagct ggtgagcgcc tgggaatata aagccgcgaa tgacacgctg gccaatcaat
     1441 tccatgacgc cggatttgat ctgaaacatc cgcaaattcg caagtttttc gacctgtgcc
     1501 tggaagttca ggatttgccg cgccatttgg ggcagcattc cggcgggctc gtgatttgtc
     1561 agggtcaact ggattcggtt gttccactcg agccggcgac catgccgggg cgcgtcgtgg
     1621 tgcaatggga taaagacgat tgtgcgtcga tgggcattgt gaaagtcgat ctgctcggcc
     1681 tgggcatgat ggcggtgctg gaggaatcgc tgcaaatcat ccacaaacaa tatggcgagg
     1741 aaatcgatct agggaaattg ccggcaaacg atccggttgt gtatagcacg ctgcagaaag
     1801 cggatacggt gggcatgttt cagattgaaa gccgcgcgca gatgtcttgc ctgccgcggc
     1861 tcgtgccaaa atgtttttac gacattgttg tgcaggttgc gatcattcgt cccggcccga
     1921 ttgtggggca aatggtgaat ccatatttga agcggagaca ggggcgggaa acggtggatt
     1981 accttcatcc ctctctcgaa ccagttcttg cgcgcacgct cggtgtgccg ttgtttcaag
     2041 agcagctgtt gcgcatggcg atgatcgctg caggattctc cggaggcgaa gcagaggagt
     2101 tgcgtcgcgc ttttggtttt aagcgctccg aagtgcgcat gaaagatatc gagcaaaaac
     2161 ttcgcctcgg catgacccgc aatcacattg cttcagagat tcaggaaaaa attattctgg
     2221 cgatcacttc gtttgcgttg tacggatttc ccgaatccca cgcggcgagt ttcgcgttga
     2281 tcgcctatgc cagcgcgtat ttgaaatgcc actttctccc cgcgttcacg gcggccattt
     2341 taaataatca gcccatggga ttttatcaac ccgcaacact ggtaaaagat gcgcagcggc
     2401 acgggctgaa ggtgaggccc gtgaatgtca cttgttcgga ttggctatgc acgctggagg
     2461 agaacggcga ggactttgcg atgcgcttgg gaatgcgctg cgtgaccggc ttgcgcagag
     2521 aggtggcgga aacaattgtt cgcgaacgcg cgatttttcc gttcacttcc attcatgacc
     2581 taaaacttcg cgttcccttt attcacaaat cagagctgac catgctcgcg gagatcggcg
     2641 ccctgaattt catttcgggc aaaaacagta ttcatcgcag ggatgctctg tggcaggtgg
     2701 aacgcgtggc ccggcgcgcg gggccgctgc tggaaaaaat agaattgcct gcaatgagtg
     2761 aaggcgaatt tcacgcggaa gaacgactgt atggcgacga gccgtcaggc ttcaaaacgt
     2821 ctcccctgaa accaatgacc tccgaggaaa aattaagagc tgacttcaac ggaacgggca
     2881 tgactgtggg cccacatccg atgagctacc accggaaaaa attgaagagg caaggcgttc
     2941 gctgcgcgag cgaactgagc agcctgccaa acgggattcc ggttcgcatt gccggcgcag
     3001 tgattgcgcg ccagcggccc ggcacggcga aaggttttgt gtttctcagc atggaagatg
     3061 aaacgggcat cgccaacgcc atcatctcgc cgcagacgta tgacacgtat catttaattg
     3121 tcgtgcacca gcaattttta ttgattgaag gacagctgca gaatcaggaa aacgtaattt
     3181 cggtgaaggc ggaagtgatt cgtccgttgc cgatcgcgag agtcgaatcc agttcgcatg
     3241 attttcatta agcacgcgaa tcggcacgtc atcagcaaac cctacgaatg cgaaggttaa
     3301 acaggctgtg gagaaagctc tgtcatttcc gggcggagtg aggaatctct ccttcttttg
     3361 tttgcggata cctagataga agagagattc ctcgctccgc tcagaatgac aaaataaagt
     3421 acgcttttta gctttgaaat ctgcccaagg tcgtttccaa ttgcataaag agccaccacc
     3481 aaatcaaaga acctcttaag gggtttgctg gattgttgga atttcttcaa aaagttagat
     3541 ttgtagtgaa taggtgtgaa aggcggatca cgaacgcaaa ttattcagcg tgccggcacc
     3601 tatttcttga atgaaggagg aatatgccgt cgagcgtttg gaaaggttac atttcctttg
     3661 gcttaatttc agtgccggtc cgactgtatg ccgccgcgcg ctattctcat gtcgccttcc
     3721 atgaaattca ccgcaaatgc ggcacgcgtg ttcatcaaca gctgtactgt ccctacgacc
     3781 aagaagttgt gacgcgcgat gagatcgcca tgggctacga agttgataaa gacaagtacg
     3841 ttctcgtcga accatccgag ctaaaaagcc ttcagccgcc atcctccacc gcaatggaaa
     3901 ttattcagtt cgtgaagctt tcggaagtcg atcccattta ttttgagacg tcctatttct
     3961 cggtgcccga agaagccggc caaaaggcct acgcgctgct gctgaacgct atggaggaca
     4021 agggttacgc agccatcgcg aaggtcacga tgcatcaacg agaaaggact gtaattatcc
     4081 ggtcgtatca gaagggattg attctgcata cgatttatta tccgagcgaa atacgcgaca
     4141 taaaaggtta tgggcaaccg acggcgcaaa atctgaaaaa acaggaaatc gatctcgccg
     4201 agcaatttgc cgcttcgctg gtcaagccat tccgtccgga gcaatttcac gacgaatatt
     4261 cggaacgagt gcaggaactg attgaaagca agagcaaagg gaaagctgcc ccgaaaccag
     4321 aaaaatcgca gcgcctggcg cccgtcgtcg atctgatgac agcgctgaaa aaaagtctag
     4381 cgggcgcgaa acattccggc ggcggctcgc ggtctagcaa gcgattgagg aaaaccgcat
     4441 agtgaaaact tcccgggctg ccaactctga ttcgaataaa aagttgaaga cgctcgcccc
     4501 cgttaccaaa gtaaggagaa ccaccgcggc cgccgcgatg cccgcggtcc cgatcgaaga
     4561 agtattcagt tttctcaagg aaaccagagg agttgtaagt tggacgatcg cagacatcgt
     4621 cgatgccttg aagatcggcg aggccgaggc caaacgaatc gtccagatac tcgagttgca
     4681 aggatatgta aaacagcaga atgactctgg cgatacagcg caatggctga cgacgctcag
     4741 cggcgaaagt gtctccggtt cggcggctcc gcggttcacc tcggatcgag tgaagaaggg
     4801 gctcgcggca ctcgccgatc gtatcagggc aatcaaccgg gaccagcacg ccgcgctaag
     4861 tattcctcag gcagtcgcgt tcggagattt tctgcgcaca cggacgaggg tgcaggcggc
     4921 ggatgttggc gtacgcatag agtcgaaacc gcacgctggt aaaaaacaaa atccgcagca
     4981 cagtgaaggc gacttcctca agcagctgaa agcacgaagc gcgctggtca acgtgcaaat
     5041 ttacaaaccg tggatgagca aacgctcgca tgttcggctc gtttgaatcg atgagacgca
     5101 tcgcgaatca ggtttttcgc gctgcaagac tcttaagcaa gcggcgcctc gcgcaggcca
     5161 caatctgctg ctcatatcgc aacttcattc ctgctcgacg agcttcggtt cgtgattgca
     5221 tcgcctatgc ggtccgggac aggtagcaaa tgcagcgcgt cggcgtcagg ctgacgattc
     5281 gcttcggcca aaatggcgac ggcatcccag tgcgcttccg cttcgcccgc ggttcgcgaa
     5341 caggtttcct aagtccgcga ctccagcttc tgaagcgcgc gcaatacgcg ctccggcgta
     5401 agcggaatat ctcgcactcg cgcacccgtc gcgtcgaaca ctgcgttggc gatagccgct
     5461 gggacaggaa tgatcgatgc ttcacctgcg ccgagaaaac ccaactccgg acgatcgatt
     5521 aggacgatat cgatttccgg gatgtcctcg aagcggatcg ttttgtaact gctccagtcc
     5581 atattcgtga cgccgttttc gtcgaactgg acttcttcaa ggagactacg acttacgccc
     5641 tggacgacgt ttccctcgat ctggttcttg agaccatccg ggttcgcgat caggccgcaa
     5701 tcgtgcgcga cggtcactcg ggtcaccgtt actttccccg tcgatttctc aacttccact
     5761 tccgcgacag ccgccacgat cgcgccatct cggtcggaca tggcagctcc gcggccgacg
     5821 cacttgtcag gattcgtctg ggctggcgcg ggcgagggac gctcaaccca gccggcctgt
     5881 ttcgccgctg cgagcaatac ttcgcttgtt cgctgttgat ttctcggaga ccgcaatcgg
     5941 aactgaacag gatcgacgcc ggcatgaaac gcaacctcat cgataaatga ttcgctggca
     6001 aatgtacggc ccacggctcc cggcgcgcga agattgcaag tccgcagatg ggacatcgcg
     6061 ttttgtatcc atggaattac gggacaatta atccgctgat tttcgaacgc gtatagatcg
     6121 ccgcttccgg tagttccgta ttcatacccg ttgcccggaa agccagggcc gttgggcctc
     6181 aatccagtct gcgtggatgc caagagcgga atgccaggcc cagcagtata aggaaaataa
     6241 cggtccatga agtcccacgc cgtaatattc ccccgcggat cgactccggc gcgtacggtc
     6301 attaattgcg cggacccctt gggttcccag gcgtgctctt cgtggcgcat ccactgcacg
     6361 cggacaggct tgttcaccgc tcgggacatc agcgcagcat cttccgcaac atcgtctgcg
     6421 cagagccgac catagcagcc cgacccttct gcgtagataa tgcgcacatt gttttccggg
     6481 aggcgcaaga ggtcagcgac ggctttgcgg gttcgatgcg tgccctgcga accggcccag
     6541 accgttgcct tgccgtcgcg cacgtcggct acggcgcagg atggaccgat cattccatgc
     6601 atgtgaaacg gccaatgata ggtagcctca acgacgcggc tggagcggga cagcgccgca
     6661 tcgaagtcgc ctcgattggc cactaattgt tccttgaagg gcttcgtttc cttaagatag
     6721 gaataaagat catcgcgact cgcgggtagc tttgcctcgg gctctgacca agccgctttt
     6781 agtgcttggg aggcgcggat tgcgctccat tcagtggcgg ccacgatccc cacaaagtgg
     6841 tcttccttca caattttcac gattccggga atgtccttga tggaattttc gtccacgctc
     6901 accagacgcg caagggaatg ctctggccga acgacgcgac cgtgaagcat gcctggaatt
     6961 cgaacatcct gcgaatacgt aaattcgccg gtaaattttg cgggcaaatc gatgcgcgga
     7021 atggaagtgc cgacgatttt gtaatccttc gcattcttcg gcgaaatttc cggcgcaact
     7081 ttcatctcgg ctgctgttcc cgtcgcggta atggcgacgt cgaagcgctt gccgccgagc
     7141 aatttccaat acgagatgtt tgtcgacgga ttgtcgacga cgctcacaac gccgtcatcg
     7201 acagcgagct tctctgccgg cacatcgaga cggacagcag cgagcttcag gagttcgtgc
     7261 tttcccgccg cagccgcttg ccgcaattgc gggccggcct tgtgcagcgt tcggcttccc
     7321 gatgtctgcg actggtcgat ggtttgcgaa gtatcgcccg tgatcatgtg gatgcgttcg
     7381 aacgatacgt cgagttcttc ggccatcact tgcgataacg cggtgagcac gccggttccc
     7441 agatccacct tgctggtgaa aacggtaatg attccgtcgg atccgataac cagccacgaa
     7501 tcgagctggg tgggcagcgg gctgccgttg aatccggatt gctcgcctaa ttccgcggcg
     7561 cgcagttgct ggccaaacaa agaaaatcca atcaccaggc cggcggcatt ttttacgaaa
     7621 tctctgcggg agatcgtagc gctcgccatg tcatgccctc cccgttttat ttcgttccat
     7681 cgccgcacgt ttgacggcgc gaacaatgcg cacgtgcgtt ccgcagcggc agagattggc
     7741 gttcaaggct tgcttgattt cggcaacgga aggatttgca ttccggtcga gcagagcctg
     7801 agccgtcatg atcattccgt tcgcgcaata gccgcactgc gcagcttgct cttcgacgaa
     7861 cgcattctgg agaaatccgg gatgggtgct gtttccaaga ccttcgagag taatgatgct
     7921 gcgcttttcc atggagctga caggaacgct gcaggagcga atgctacgcc cctccgacaa
     7981 taccgtacac gccccgcact gcgacattcc acagccaaat ttgggtccgt tcagtcgaaa
     8041 ttcatttcgc aatacgtata gcagcggagt actcggatcg acgtcaatcg tccgggcctt
     8101 tccgttcacg gtaagagaaa tccgcgacat aattgcaacc tccgtatttc ggtaaatccg
     8161 gcacggcaac gggaagccca taccattcgg aatccggcga gcgacgggat gattcatccc
     8221 atcgtgatct gatgcgccaa cctccgcccg cgggcaattc aagtttctgc agtaactgtg
     8281 aagtgcggag agagattgcc cctatggtcc ccgaattgag cgggataaag ccgctttgcg
     8341 cgcgataact caattctact tcatggttac aaattcagat tcgtgaaaat cagcaaactt
     8401 ccaataccca tcctgtttga ccaagacaaa tgtaaaaatt cccttgcgaa ccggattcgc
     8461 cgaaccatct ccggccaaag atccctcgag cgcccactcg gcgtcggcga acgcgacgtc
     8521 aggcttcgca aatcgaaggt gcctgacggt aaccgttcga tgagcgtttt tgagaggacc
     8581 ggcaaataaa accttaaaac gctcttccac ttcttttcgc ccgtgttttg tcaggccgga
     8641 aacgtttgta aaatctccgt catcaccgaa gggcatggcc catccagctg cgtcatgccg
     8701 gttaaatgct cctacaaaat cgtcaataac cttccggatc gctgccgagt ctgcatccgt
     8761 tgaagtcgat tgcgcatgaa cccgcaagcc gggcatcgcg acggaaaaaa tcaaaagaac
     8821 agaaagagct attacagatc gcgtccagtt cattttcacc gccttaagac tcgcagtctc
     8881 tatcataaat attataaagc ttctgccgcg ttaaaaaaaa cgagttgaac gctccgtatg
     8941 cgaaatgatt tacccgcaga ccgtcagacg cttcatctcg gccgtccact cccccgtgcg
     9001 aaagccgtgc tgcatatgat agaggtgccg aaccaggtag ctcggagagc gtccagcaag
     9061 gcccgggcag ggcgcggtca cgttttcacg attaaaacat ccatcgttcg aggaccattt
     9121 cccgcgtaag ttggctggga tttttcatag aaggcgccac ggtagaaagg tcggtatgat
     9181 cgccttatca cctcgaatca tatatgcggt gcaaattcag atcctgcacg gcatttttat
     9241 tttggatttc ctcatttacg ggtttttctt ttgcgatttt tttgtcggat aatcgcaatc
     9301 ttctgacgaa acctcaacgg aagctatagg ctcaacgcct atcgaagcag agccgcaggg
     9361 agaagcagtg cctggaaaca ttttaatctg gctagcataa cgagattatc cctgtgcgtt
     9421 tcatcgttca aggaaaccgt cattccgcga taggggcgaa cttaagtaca cgaaattatg
     9481 attcttaata cgtaggtctg cctatattcc cgccgcattt gtgtgggcaa tatcagttcg
     9541 gtggtggtcc taaattcaat gtctatatgt tcggaggagt tatggggtat ttatccgttg
     9601 tgtggcggtg cgttcgcgcg agcttattct tttctttttt ttgtttcatg tttatttgtt
     9661 ctcctaatca cctgcatgct caaggcctgg aggtaaacgg cggatatgcg cactccacgg
     9721 gggacttcgg cgtggatggt ttcgaggcag gtgccggttg gttctttgct cacagagtgg
     9781 ctctcgtcgc tgattatgac agcctttggg atacttcgcg tataggggcg tttgagttca
     9841 catccgtcgg agcgatctca tccaagagcc atttgcagaa ttttctcgtc gggccgcgag
     9901 tctatctgag gacccgaaat gttgacaaat atcccataac gcctttcgcc gaggctcagt
     9961 ttgggctatc gcatttaaac tccacaattc aggaagggac agccgtaccg ttctcggcgt
    10021 cggatacggc attcacttgg atgctgggcg gcggggctga ttatgcgctt agctctcact
    10081 ggacggcacg cggtcaactc gacctgctgc gtactcatct gaatgctgaa ggacaaagca
    10141 ggctcaggat tggaatcgga atcgcttatt cgttcggaca gcgctaattt ggcacgatcg
    10201 agacatgaag tattcgaccg gcttggggcg tgtcatgcgt tcggggttgc ctgcaggttt
    10261 tcgacctagc aatcccgaac atgacctgct ggggataaaa ccccttcgac agcatcgtgc
    10321 ctaaggagtc gggtaatcta gccgacccat ctcgcttgag tgccattgac gacgacgatg
    10381 accgagtgtt tacagtcgtc atcgagaccc cgcggggatg tcgaaacaaa tacgcgtttg
    10441 acccagacca gagagtcttc agcctaaaaa aagtcctccc tgcgggcatg gcatttcctt
    10501 atgactttgg ttttgtgccg tcgacagaag gggaagacgg cgacgcgata gatgttttgg
    10561 ttcttatgga cgaaccagcc tttcctgggt gcgtcgtgcg gtgccgttta attggagtta
    10621 tcgagggcga acaaaaggat ggcaagaacg tcgaacgcaa tgatcgcatt ctcgcggtag
    10681 aaatcggaac tcattcgtgg gacgacgtcc ggcacgttga tgacattgga aagcagtttg
    10741 ccaaggagct cgcaaagttt tttgtacact atcatgaatt aacgggcaaa gagtatcggg
    10801 ttctcgccat gaaggagcct ggcgcggcca caaaatgcat cgcgaaaggt atgaaggccg
    10861 ctaagtaggg ctcgcgatag gcaaatgata aagcattcgc gctccccaga ctgccgcatg
    10921 acttacggtg tctaagctaa aaaaataaac tccgggttta ttttgcacgg ttacataagt
    10981 ccggaaatta agttcaggaa ttgctgacat acctggcaga acaggcaagc ctcggagacg
    11041 aatggacatg tggaacggcg taatgctgat ccaaccctc
//
LOCUS       DASZMB010000007         5825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1655187, whole genome shotgun sequence.
ACCESSION   DASZMB010000007 DASZMB010000000
VERSION     DASZMB010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655187"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..1164)
                     /locus_tag="VG272_00150"
     CDS             complement(244..1164)
                     /locus_tag="VG272_00150"
                     /inference="COORDINATES: protein motif:HMM:NF024931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HWF12117.1"
                     /translation="MHFKNFSAAVFGIDAYLMEVEVDVGSGRPGDFNIVGLPDIAVKE
                     SRERIKAALKNCGHEFPLQSVTVNLAPADIRKEGSALDLPMALSILGCEGAFFGKQLD
                     QYIFLGELALDGQVRSVRGSLSAALAAREHKIRAVVVPEANAREAAVVEGIDVFAVRS
                     LPQAVDLVNAPESFVPVRVDASSMLADASQYAVDMRDVRGQQAAKRALEVACAGGHNI
                     IFSDGISNVQPVPRNGRGRELFKTPNDAVQVQSLSGFRPKELLVSNFHQGLQRSAAHD
                     HAGFPVPLLDATKSLLHRGFDLLRHFRSPA"
     gene            1393..1956
                     /locus_tag="VG272_00155"
     CDS             1393..1956
                     /locus_tag="VG272_00155"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HWF12118.1"
                     /translation="MLIGLVVLVVGVVLYLMFTYNGLVRLKVQCDNAWSDIDVQLKRR
                     YDLIPNIVETVKGYAAHEKSTLEDVVAARNQAMSAQGPAAKGEAEGLLTGALRQVFAL
                     AEAYPQLRAVESFTQLQGTLNQIEDTLQNARRYYNAVVRDFNTMIEQFPSNIVAGMGN
                     FKEREFFQISAPAEREVPKVSFGTTGA"
     gene            1962..3752
                     /locus_tag="VG272_00160"
     CDS             1962..3752
                     /locus_tag="VG272_00160"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HWF12119.1"
                     /translation="MKNRAETAISCASTAELRGSLRARFASVAAILLLALLFAATASA
                     REIKIEKFSAEIFVQQDSTLDVTETIEANFIGAWHGLYRTIPVEYVTPQGFNYSLFVK
                     LEGATDAAGQPLKVESSRQGHNLKWKIYIDDATDVDRTIHLHYRMRNGLKFFEDHDEL
                     YWNITGEEWDIPIDDVSAQILLPTGVTGVRTNEFTGSYGSPSQGASISASDNTIEVSM
                     NRPLAFHEGLTVAVAFDKGFIKEPGTADLIGQFFESNWPIFLPILVFFFMFWLWSTRG
                     RDPRVGPIAVQYAPPDGMTPAEVGTLIDEQTAMRDITATIVDLAVRGYLAIEEKEKSG
                     IAGMLLHSKDYTFHLKKGLPEWPGLKAHELALLGGIFSNGAQTDVNLSSLQNVFYKSL
                     PGIKNGIFDALMKHGYFQHRPDYVRSGYIVGAAVIGVAIFLTGNWLSQKTGVALIPFL
                     VAGVASGIIIAGFGWFMPARTSDGAKALAGILGFEDFLTHVESDHMSRVSQTPETFEK
                     FLPFAMALGVEKKWVGAFQNIYSQPPSWYQGNYSGGGFYPIMFVNSLDHMTMSASSVM
                     ASAPRSSGGSGFGGGGFSGGGFGGGGGGGF"
     gene            complement(3955..>5825)
                     /locus_tag="VG272_00165"
     CDS             complement(3955..>5825)
                     /locus_tag="VG272_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HWF12120.1"
                     /translation="GVWSFQFRSAVEHQRGIWVILIPVIGGLLIGLMAKYGSPKIRGH
                     GIPEAMEAVLVSKSRIEAKVAILKPISAAIGIGTGGPFGAEGPIIQTGGALGSLVGQL
                     FSTTASERKVLLACGAGAGLAAMFNAPITGALLAIELLLFEFQARSFIPLMLASALSV
                     AVRLRLFGDETMFPVGSFNYDLIHGIPFYLVLGLICGFAAIGFASTLKRLEDFFDHMK
                     KPPAVFHPAIGALGLGVVGYFLPRVFGPGYATISDVLNVRMTLTAVLLLLIFKAFVVL
                     LSLGSGTSGGTLAPMFMMSGAIGSTFAMVVNHIIPSAHLSVGAYAVAAMGALLCACAR
                     APFTFLVCAIETTHDFHAVAPVILVCIVADAIAVRYMPHSIMTEKLERMGFGAGQDYE
                     SNSLKQLRVADVMVRDVATVLPEETVRSAADRFASGDLQNSRHHALPIVDSEGRLRGL
                     VTQGDLLRALQSDPEGKIGVLDAGARSLVVAHADERVFDAVIKMLENNIGRLPVVDRK
                     DPQKMVGYINRANVMGSWRGHLQAESVREHGWLNNLRSISDRKGNHGVTVGRVASLTE
                     KHLLLAPSADSDEPSDVYVLSAPVRGVLLGDEVRVSYEQNDGRKVAIRIEELSSRQ"
BASE COUNT         1384 a         1655 c         1545 g         1241 t
ORIGIN      
        1 gcaattcggc tcacaatcag gaagctcgaa atggaggagc aagcgttggt gctttgccat
       61 atcgaagcgg atgggtcgct cacgattttg agtaacagca aagactgcgc cacgaggttc
      121 ttcaacagcg ttttcttgga cattccgccc tcatttcctg ctcgcaagga ttggagactc
      181 aagggcggct gggtaaacga cgagagcgct gtagcattct tcgcgggggt ggctgcgggt
      241 gcgttaggct ggggaccgaa agtgccgcaa gagatcgaag cctcgatgaa ggaggctctt
      301 cgtggcatcg agaagcggaa ctggaaatcc tgcgtggtca tgtgccgcag agcgctgcaa
      361 gccttgatgg aagttgctta cgagaagttc tttgggtcga aacccggaaa gggattggac
      421 ttgaacggca tcattcggag ttttgaaaag ctctcgcccc ctcccattcc tcggcactgg
      481 ctgaacattg ctgatgccgt cagaaaaaat aatgttgtgc ccgccagcgc aagcgacttc
      541 gagcgcgcgc ttcgccgcct gttgcccacg tacgtctcgc atatcgacag catattgcga
      601 agcatcggcc agcatcgagc tcgcgtccac gcgcaccgga acgaaagact ccggcgcgtt
      661 cacgaggtca acagcctgtg gtagcgagcg cacggcaaac acgtcaatcc cctcgactac
      721 agcggcttcg cgcgcattcg cctccggcac gaccaccgct ctaattttgt gttcacgcgc
      781 cgcaagcgcc gccgaaagcg agccgcgcac cgagcgcacc tgcccgtcaa gcgcgagctc
      841 gccgagaaaa atatactggt cgagttgctt gccaaaaaaa gcgccctcac atcccagaat
      901 tgataacgcc atcggaagat cgagtgccga gccttccttg cgaatgtccg cgggcgcaag
      961 gttcacggtc acgctttgaa gaggaaattc gtgcccgcaa tttttgagcg cggccttgat
     1021 ccgttcgcgg ctctctttta ccgcaatatc tggcagccca acgatattga aatctccagg
     1081 cctgcccgag ccgacgtcca cctcgacctc catgaggtaa gcgtcaatgc caaaaacagc
     1141 cgcgctaaag tttttgaaat gcatgtgtca ccgggcgtga cacgtccggt gcgcgcaaga
     1201 gtagcgcaaa tgaacttgtc ttactagcga taatgccggt tactcaagaa cgaaagattg
     1261 aaagctgact taggctgaaa tactgcgatt ctcgcacatc caaatcacgc gcttctttac
     1321 tgcatttaac aggtctctta tagtgattta aagcatcggt cgcgccattc accgatcgcg
     1381 aaaggatact gcatgttaat tggcctggtc gttctagtcg taggtgtagt gctgtacctc
     1441 atgttcacat acaacggatt ggtgcgtttg aaggtacagt gcgacaacgc gtggtccgat
     1501 atcgacgtgc aattgaaacg ccgctatgac ctgatcccca acattgtgga gaccgtgaag
     1561 ggttatgccg cgcatgaaaa gtccacgctg gaggacgtcg tagcggcacg aaatcaagcc
     1621 atgtccgcac agggcccggc cgccaaggga gaggccgaag gcctattgac cggagcgctg
     1681 cgccaagttt ttgcgctcgc cgaagcctat ccgcaactgc gcgccgtcga gagcttcacg
     1741 caattgcagg gcacgctcaa tcagattgaa gatacgctgc aaaatgcgcg ccgctactac
     1801 aacgccgtgg tgcgcgattt caacaccatg atcgaacaat tcccttccaa catcgtggct
     1861 ggaatgggca acttcaagga acgcgaattt ttccagatca gtgcacctgc ggaacgcgaa
     1921 gtgccgaagg tcagcttcgg cacgacaggc gcatagcccc aatgaaaaat agggcggaga
     1981 ccgcaataag ctgcgcttca actgccgaat tgcgcggatc cttacgcgcg cgattcgcga
     2041 gcgtagccgc aattctgttg ctcgcgcttt tattcgcggc cacagcttcc gcacgcgaaa
     2101 tcaaaatcga gaaattttcc gctgaaatat tcgttcagca agactcgacg ttggacgtga
     2161 cggaaacgat tgaagcaaat ttcatcggcg cgtggcacgg gctgtatcgc acgattcccg
     2221 tcgaatatgt aactccccaa ggcttcaact attcgctttt cgtcaagctc gaaggcgcca
     2281 cggacgccgc aggccaaccc ctaaaggtag agtcctcgcg ccaaggccac aacctgaaat
     2341 ggaaaatcta catcgatgat gcgaccgatg tggatcgtac cattcacttg cattaccgga
     2401 tgcgcaacgg cttgaaattt ttcgaagacc atgacgagtt gtactggaac attactggtg
     2461 aagagtggga cattcctatc gatgacgtca gtgcgcagat tctgcttccc acaggcgtga
     2521 ccggagttcg gaccaatgaa ttcaccggat cctacggctc gccctcccag ggtgcgagca
     2581 tttctgcctc ggacaacaca atagaagtgt ccatgaaccg cccgctggcg tttcacgaag
     2641 ggctgacggt ggctgtggct tttgataagg gcttcatcaa ggagcccggg accgcggatt
     2701 taatcggaca attttttgaa agcaattggc ccatttttct tcccatactc gttttctttt
     2761 tcatgttctg gctctggtca acgcgcgggc gcgacccgcg cgtcggccca attgccgtcc
     2821 aatacgcgcc tccggatgga atgactccgg ctgaagtggg cacgttgatc gatgagcaga
     2881 ccgctatgcg cgacatcaca gccacaattg ttgaccttgc cgtgcggggc tacctcgcta
     2941 tcgaggaaaa agagaaatcg ggaatagcgg gaatgctgct acacagcaag gattacacct
     3001 tccatttgaa aaaggggctg ccggaatggc ccggcctgaa agcccacgag cttgcgctgc
     3061 tgggcggaat tttttctaat ggtgcgcaaa cggatgtaaa tctttccagc ctgcaaaacg
     3121 tgttctacaa aagtctacct ggcatcaaaa atggcatttt cgacgcgctg atgaaacatg
     3181 gatatttcca gcaccggccc gactatgttc ggagtggcta tatcgtagga gcggccgtga
     3241 ttggcgtcgc tatttttttg acgggtaact ggttgtcaca aaaaacgggt gtggccttga
     3301 ttccattttt agttgcggga gttgcttcgg gcataattat tgccggattc ggctggttca
     3361 tgccggcgcg cacatctgac ggagcaaaag ctttagcagg catccttggt tttgaggatt
     3421 ttctgacgca cgtggaatcc gatcacatga gccgcgtcag ccagactccg gaaacgttcg
     3481 agaaatttct gccattcgca atggcactgg gagtggagaa gaaatgggtc ggcgcatttc
     3541 agaatattta ttcgcagcca ccctcctggt atcagggcaa ttattcaggc ggcggcttct
     3601 accccatcat gttcgtcaac agtctcgatc acatgacgat gagcgcaagc tcggtcatgg
     3661 cttccgcgcc gcgcagcagt ggtggatcgg gctttggcgg tggcggattt tcaggcggcg
     3721 gcttcggcgg cggtggtggt ggcgggtttt aagtagcgcg cacttcagag tctgtgtgag
     3781 gacttcaaac tcgttgtggg tcgtggcttt agccacgaca taagtgctgc aaaataagtt
     3841 cggctttagc ccctgaggtt ccaacgttgc agttttctca cagactcttc agcctgcgga
     3901 atttagacct ttcaagcact aacctccgca gtctaaagcc cgcgctactt ggttctactg
     3961 cctgctcgaa agctcttcga ttctgatggc aacttttcgg ccgtcattct gctcatagct
     4021 cactcgcact tcgtcaccca gcagaactcc ccgcacaggt gcactaagca cataaacatc
     4081 ggaaggctcg tcggagtccg cacttggcgc gagcagaagg tgcttctccg taagcgatgc
     4141 aacgcggccc accgtgacac cgtgatttcc tttgcggtcc gagatcgacc gcaggttatt
     4201 cagccaacca tgttcgcgca ccgactcggc ttgcaggtgc cctcgccacg agcccattac
     4261 attcgcgcga ttgatatacc ccaccatctt ttgcgggtct ttgcgatcca ccacaggcag
     4321 gcgtccaata ttattttcca gcatttttat cacggcatcg aaaacgcgtt catcggcatg
     4381 cgcaaccaca agcgaccgcg cacccgcatc cagcacgccg attttcccct ccggatcgga
     4441 ttgcagcgcc ctcaacaaat ctccttgggt cacaagtcca cgaagccgcc cctcggagtc
     4501 cacgatcggc agcgcatggt gccgcgagtt ctgtaaatcg ccactcgcaa atcggtcagc
     4561 agcgctgcga acggtttcct caggcagtac agtcgcaacg tcgcgaacca tgacgtccgc
     4621 aactctcaac tgcttcagtg aattcgattc gtaatcctgg cccgccccga aacccatccg
     4681 ctcaagtttt tccgtcatga tcgagtgcgg catgtaccgt acggcaatcg catcggccac
     4741 gatgcaaacc agaatgaccg gagccactgc atgaaaatca tgcgtcgtct caatcgcgca
     4801 aaccagaaac gtaaacggcg cgcgcgcgca tgcacacaaa agcgctccca tcgccgccac
     4861 ggcatatgcg ccaacggaaa gatgcgcgct cggaattata tgattcacga ccatggcgaa
     4921 cgtgctgcca atcgcaccac tcatcatgaa catgggagcc agggtgccgc ccgaagtgcc
     4981 ggaaccgagc gataacaaaa cgacgaaggc cttgaaaatc aggagcagta gtaccgccgt
     5041 cagagtcatt cggacattta acacatcact gatggtggcg tagccgggcc cgaacacacg
     5101 cggaagaaag tagcccacga cgcccaatcc caacgcgcca atcgccggat ggaatacggc
     5161 tggcggtttc ttcatgtgat cgaaaaaatc ttccaggcgc tttaaggtcg atgcaaaccc
     5221 gatggccgca aaaccacaaa taaggccaag gaccagataa aaaggaattc catgaatgag
     5281 gtcgtagttg aaagagccga ccggaaacat agtctcatcg ccaaacaagc gaagccgcac
     5341 agccacggag agcgcgcttg ccagcatcag cggtatgaat gaccgtgcct gaaattcaaa
     5401 aagtaaaagt tcgatggcca gcagcgcacc cgtaattggc gcattgaaca tcgccgcaag
     5461 gcccgcacca gcaccgcaag ccagcaatac tttccgttcc gacgccgttg tcgaaaacag
     5521 ttgcccgaca agcgagccga gcgctccccc tgtctggatg atcggccctt ccgcgccgaa
     5581 cggtcctccc gtgccaattc cgatggccgc ggaaattggc ttcaggattg caacttttgc
     5641 ctcgatgcgg cttttggaaa ccagaacggc ttccatcgcc tcgggaatac catggcctct
     5701 aatttttgga gatccgtact tggccataag tccaatcaga aggccgccaa tcacaggaat
     5761 caagatcacc catattccgc gctgatgttc cactgcgctt cggaactgga atgaccagac
     5821 gccgt
//
LOCUS       DASZMB010000008        14013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2760527, whole genome shotgun sequence.
ACCESSION   DASZMB010000008 DASZMB010000000
VERSION     DASZMB010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 14013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14013
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2760527"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..1147
                     /gene="surE"
                     /locus_tag="VG272_00170"
     CDS             350..1147
                     /gene="surE"
                     /locus_tag="VG272_00170"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HWF12121.1"
                     /translation="MAHILITNDDGIQAEGLRALVQAIEGLGMVSVVAPSHERSASAQ
                     SLTLRHPIFWDQVAENEWSVEGTPADCVILALNKLLPNRPDIVISGVNRGGNMGENIF
                     YSGTVGAAMEAAINHIPAIAVSVVHKGKGFRFEHAAKLARDIAQIVIAEGMPLSTVLN
                     VNVPLAWNGNVRATRQSRKVTRNVLQEGTDPRGRSFFWLLEQEHIEGVDPKSDYAAVF
                     DGAASVTPLHLDRTHEISLKHVSEWAARLEGNAENAEPATVTRNTLK"
     gene            1312..1704
                     /locus_tag="VG272_00175"
     CDS             1312..1704
                     /locus_tag="VG272_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF12122.1"
                     /translation="MKHRILLIDSDPQSRELLRSWLESEGHQTILAETLEEGFVNIAN
                     PPLPSVVLLDIQLGNQSGLTLVHWARRQPHLANVPIAAMSTFASFKEFKRVEEAGCDT
                     CFTKPIDFRSLRNYLFSVHVYSASKINT"
     gene            1757..3850
                     /locus_tag="VG272_00180"
     CDS             1757..3850
                     /locus_tag="VG272_00180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HWF12123.1"
                     /translation="MLHDFIVNPNSNPSAPLFPSPVEFDREVCGDLHDSESREWLVTN
                     GIGGYAFGTMAGLQTRSYHGFLVAALDPPLGRTLLVAKLDETARYASKDFALATNRWA
                     DGTLAPQGFLNIERFHLEGTTPVWTFSLADALIEKRIFMQSGENTTYVLYRVIRASAD
                     VEISVKVLADYADEHCVTAKHTFPMTVTTVERGLKVAAFDGTAPFFVLSDAATAIPTS
                     NWYRNFDLAAERARGLPDLTDHFYAGELRATIAPGGSLTMVASTSATASLDGEAAFAK
                     RQKEAAALLKHFSAANIAITKENSQPALTSVQQLVLAADQFIASRPLEDAPNAKTILA
                     GYPWFHDWGRDTMIALPGLCLVTGRPWLARNILQTYSRFVSEGMLPNQFPGAGVAPVY
                     NSVDATLWYFEALRQYFEATNDIGLLHELYPVLEGAIEAHQRGTRYHIHVDPADGLLF
                     AGEAGVQLTWMDAKVNGRVVTPRIGKPIEVNALWLNATATMAGFAGILGRNAARYETL
                     RTSAKAGFAKFWNSEKQFCFDVIDAPGSVDGKDAALRPNQIFAVSLPETALDAKQQRA
                     VVDICASELLTSFGLRSLGQNEPGYCGHYGGGMEERDDAYHQGTVWGWLLGPFALAHL
                     RVFGSAQQAMSFLEPLFGQIKSAGLGTLSEIFDGDPPHIPNGCIAQAWTVGEMLRAWK
                     NIAAARENEEGKPRK"
     gene            complement(3919..4764)
                     /locus_tag="VG272_00185"
     CDS             complement(3919..4764)
                     /locus_tag="VG272_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12124.1"
                     /translation="MQAPPDSPAQNQQGPPSQDRPAGQAQGQSNQPLPPETLTLPAGT
                     VVRVRVDEWISTDRNVTGDSFSAVLDQPLVVNGWVVARRGQAQTGRVSLVKKGGHGNG
                     NSQLGMDLPDLTLVDGQQLPLQTELFQTSAGSSTGRNVATVGTTTGLGAIIGGIAGGG
                     TGAAIGAGIGATAGIAGVMSTPGKPTTISPETVLSFRLKNPVTISTVNSQFAFQPVSQ
                     EDLDSRPRQNRPQMRRPGPPPYYGYPYAYGYPYGYGYPYGWYPAPVIGLGFYGGYGRF
                     GGFRR"
     gene            5541..7772
                     /locus_tag="VG272_00190"
     CDS             5541..7772
                     /locus_tag="VG272_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWF12125.1"
                     /translation="MRPKWQIAILTICLFSAIFIAARARSQSVPAAAAAQQIDSKQSS
                     SGQSGYVLKVTTRLVTLDVIATDSHGNPVRDLKPEDLQIFEEHKAQQKIEHFEYFETL
                     KRPGTVENAASAIRKASGIISNQLPLDQLKIPPTVLLMDSLNTQTPNQAQGRAKMIQL
                     LRGLPPDTPVAVFLLGSSLRILQGFTSDGRLLRAALDRSLVGNSIQQDPRNDLGDTSN
                     YMQDTTAGTGSNLGLAAQLGEVQNFEKEEYSLSVDLRMKETLGALTQIGQYLSGIPGR
                     KNLIWVSESFPLTIVPDPTTGNNPFGGGREYSDEIRIVGNRLTDAQVAVYPMDVRGLQ
                     AQQSLSASQNVSLPETRLTGPAIADRLNVEDADLQQTLGTMDEIAQDTGGKSCKNGND
                     LSGCVMTALKDSSSYYEMSFYPSKANWDGRFHKIVVKTSRSGVKLVYRRGYYALDAAS
                     LAKQQQPSDHLRQACADQLPSTTLPLTVQPLTPSQYGNAQGGLHYMLMISAAGLSATQ
                     TGDSYNLNIRVADCEFANKATVFRFNEREMPKTVSSELFRSWQSTGIPDNVALMPSSD
                     TQRIRLVVLDVASGLTGAIDIPVLPSEIAKATAPIVPPKPVVTTPYVEFPDKVKGPSQ
                     PKIVESLTFSLNSNASGTLNWKGDSLVYQLAGDMPLDRAANAFSGYAFGGRFHCQDGH
                     FVPVEASDGDAALRFTFHNHSGKLLVVDLKGEQPVYVGDLPVDPSAQTFFEQVWKASH
                     CQE"
     gene            complement(7842..9203)
                     /locus_tag="VG272_00195"
     CDS             complement(7842..9203)
                     /locus_tag="VG272_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF12126.1"
                     /translation="MERAATVSEMIDQRPLSRYQIWTMAVCAMVVVLDGFDTQSIGFL
                     APSMAETLHVPIKNFGPVFGAALLGLMISSMLIGPIADRWGRKWPVVICTLIFGAFAM
                     LTARATSLPELWTIRFVTGLGLGGALSNVVALMSEYAPKRLVAVLVSALFCGMPAGAL
                     LGGLISSVMLPRWGWQSVFYAGGVLPFVLALLLIAILPESVRYLEVSGANQNKISKIL
                     KRISPDLGDAPFSRSPKLSKHKNAPVIRLFTEDRAAGTILLWIPYFMNLLILYFVVSW
                     LPALLRQRGLPVSAGITAIILFSVGGILGSAAEGVLINRWGAFTILLAEFVCSAALIG
                     SLAYSASFPLTMTITFVLGFTVQAAQGGLNVIGATIYPTSIRATGIGWALGVGRIGSI
                     VGPVLVGVMLKLDWGPREIFQAGAIPALCAAAATLISLWLRRNPPANQPSRDILEEPT
                     LSH"
     gene            complement(9368..10150)
                     /locus_tag="VG272_00200"
     CDS             complement(9368..10150)
                     /locus_tag="VG272_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12127.1"
                     /translation="MKSRILSSAVTCAVLLFSSSLFAQTSGKETKVMIRAVARDAKVI
                     GTHVGGARITVKDVATGEILAQGMQQGGTGDTNTIMKTPHTRGMSVYNSPDASGYLAV
                     LHIDKPTVVEISAEGPLGNAQATQRTSKTLLLVPGEDVLGEGILLEIHGFIVTAVSPL
                     PDAKVKAGSPFEVKATIMMACGCPTEPDGLWDANKIHVIARLLRDGKVQSEIPMTYAG
                     VQNTFHADVPATAAGPLELQVLSMDAGSANFGMMKEAVTIIP"
     gene            10661..11974
                     /locus_tag="VG272_00205"
     CDS             10661..11974
                     /locus_tag="VG272_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWF12128.1"
                     /translation="MKPDSDPNLKKPTRRKFIRTGLLAAAAPALAVWPANAVQNAAQE
                     PSPQQASPEFTGPGANPYWNSVGPIATYPQKAPLILLTDRPVELETPRHYFLSTFTPN
                     EAFFVRWHLQTIPNAVDLKTWRLHVEGNVERPLELSFPDMLSRFKSVTVAAVNQCSGN
                     SRSRLQPRVAGGQWGNGAMGNASWTGVKLREILSAAGIKSGSVQLQFEGLDKGLGPKG
                     FGSNRFMKSLDLTNPVLDECIVAYAMNEEPLPVLNGFPVRLIVPGYFATYWLKSLTWI
                     RVLETTDDNFWMKGAYRIPETPRGNTNPEEMKSGSVKTVPITKMPVRSFLISPDGSRK
                     IPAGFPLKLRGIAFSGHGGIKRVEVSADNGSNWSDARLEQDAGEYSFRTWSLEWTPRS
                     PAQYQIAIRATDTKGNVQTDERVWNTGGYLWNQIERQEIVVGEAS"
     gene            11987..12400
                     /locus_tag="VG272_00210"
     CDS             11987..12400
                     /locus_tag="VG272_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12129.1"
                     /translation="MHKRKWILVLAIGLLAMLLGQGIYADFRAGYYNPAGPETMHQIL
                     PASASTDSTYTAGQYPIYYPELADGDGKTEVENNCELCHSPRYITMQPPMSAQAWAVE
                     VNKMRNIYGASIPEATAQKIINYLGTHYTPETRKR"
     gene            12449..13261
                     /locus_tag="VG272_00215"
     CDS             12449..13261
                     /locus_tag="VG272_00215"
                     /inference="COORDINATES: protein motif:HMM:NF044772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWF12130.1"
                     /translation="MIRVNAVRGIILAALTVSAIGWFSATSVSYGAPRTPQAADTSKA
                     WVAPNPSTIPAGPLGDSIRLGMNVFNDTPKYAERYVGNKLSCTHCHIDGGTVSQGMPM
                     VGLPGIFPMYRDREKTVITFEERIEQCFQRSESGHRIPNDSPEMAGLVAYAQWLSKDQ
                     VTGRPFPGRGLVNLPELTGDAANGSKIFGEQCAVCHGTDGAGKLPVAPALWGPNSYSD
                     GAGMNQISKMAAFVQHNMPQNAPDTLTPQEAYDVAAFIRSKPHTVFVMKDHM"
     gene            13335..>14013
                     /locus_tag="VG272_00220"
     CDS             13335..>14013
                     /locus_tag="VG272_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12131.1"
                     /translation="MKMKVNLMRVAVMCSGLAVAMGLLFLPSASAQDNAPKFEVDPMW
                     PKPLPNLWVTGGVGGLCIDSHDHVFILNRRDLTDNDLDAGRQAPVVIEFDPGGNVVNS
                     FGDPTTTPNTPHGCTFDKDNNLWITGTGDGIAQEYTHDGGKLLRQIGKMGTVDSSDGT
                     GKGKPLNSSHSSFFKPAGIAIDPKNGDVYVADGETLGGNHRVAVFDAEGKFLRQWDLS
                     RTEAETGN"
BASE COUNT         3308 a         3687 c         3861 g         3157 t
ORIGIN      
        1 cgtgaggtcg ctggttcaaa tccagtcgtc ccgaccattt tctcctccct tttgcgagct
       61 tgccagacat acggaatcgc agcacaggaa actccatctt tctgcgcctt gttgtgccat
      121 gcgccggatt gttaaactta ccaggtagag tggattggac ttcgcgtgat atgagtagtg
      181 atgattggat ggccggcttt cttttgacac atgcgtgaag tgaatttcga aatatgggtg
      241 agctcgcaag tttttaggct tccactaatg cgaaaggctt cactcttttg ccatttcgat
      301 ctcacgagaa tctaaaccgg acaggctgaa gcctatccta cgcaaaccta tggcacacat
      361 tctgattacg aacgatgacg ggattcaagc ggaaggcttg cgcgcgctgg tgcaggcgat
      421 cgaagggctg ggaatggtca gcgtggtggc gccgagccat gaacgcagcg cgtccgcgca
      481 gtcgttgacg ctgcggcatc cgattttttg ggatcaggta gcggagaatg aatggtcggt
      541 cgaaggcacg ccggcggact gcgtgattct ggcgctgaat aaattgttgc ccaaccggcc
      601 ggacattgtt atttccggcg tcaatcgcgg cggcaatatg ggcgaaaata ttttttattc
      661 cgggacggta ggcgcggcga tggaagcggc gatcaatcac attccggcga ttgccgtgtc
      721 ggtggtgcat aagggcaagg gattccgttt cgagcacgcc gcgaaattgg cacgtgatat
      781 cgcgcagatc gtgattgccg aaggcatgcc tctgagcact gtgctgaacg tcaatgtccc
      841 gttggcctgg aacggaaacg tgcgcgcgac gcgacaatcc agaaaagtga cgcgcaacgt
      901 tttgcaggaa ggcacggacc cgcgtggacg cagctttttt tggttgctcg agcaggagca
      961 tatcgaggga gtcgatccca aaagtgacta cgccgcggta tttgatggcg ccgcttccgt
     1021 tacgcccctt catcttgatc gcacgcatga aatctcgttg aaacatgttt cagaatgggc
     1081 tgccaggctg gaaggcaatg cagagaatgc cgagcctgca accgttactc gaaacacttt
     1141 gaaataaagc gcccgcaccg ttccactttg aaacgttgga gattgatcgg ctcgtacttc
     1201 gatgccgatt caagtctcga ttcgagattg acgataattg gatcgggtat tggtgtcggc
     1261 ccgcgcccag gaatggacaa caatgacttt gctcaaatta gaggaagcga agtgaagcat
     1321 cggatcctgc taatcgatag cgatccgcaa tctcgcgagc ttctacgaag ttggctcgag
     1381 tcagaaggac accagacgat tcttgcggaa acactggaag aaggattcgt aaacattgcg
     1441 aatcctccgc tgccaagcgt cgtcctgctg gatattcaat tgggaaatca gagtgggctc
     1501 acattagtgc actgggcgcg caggcagcct cacctcgcga atgtgccgat cgcggccatg
     1561 tccacatttg cttcgttcaa agagttcaaa agagtggaag aggctggctg cgatacttgt
     1621 tttacaaagc caatcgattt caggtccctt cgcaactatc ttttcagcgt gcacgtttac
     1681 tcggccagca aaattaatac gtaataggaa gtatctcaag tgcctctaaa tatttatctg
     1741 gccgaaaacc tccagattgc tacatgattt catcgtgaac cccaatagca atccttcggc
     1801 accgctgttt ccatctccag tggaatttga ccgtgaagtt tgtggcgact tgcacgattc
     1861 ggaatcgcgc gagtggctgg taacaaatgg aattggcgga tacgcgtttg gcacgatggc
     1921 gggcctgcag acgcgcagtt atcacggatt tctagttgcc gctctcgacc ctcctctcgg
     1981 ccgaactctg ctggttgcaa agctcgatga aacggcgcgg tacgcgtcca aggattttgc
     2041 gcttgcgacc aatcgctggg cggacggaac gctggcccca caaggtttcc ttaacattga
     2101 gcgctttcat ttggagggga caactcccgt ttggactttt tccctggctg acgcgctcat
     2161 tgaaaagcgc atcttcatgc aatccgggga gaacacgacg tatgtgcttt accgcgtgat
     2221 ccgtgcgagc gcggacgttg aaatttcggt caaggtactg gcggattatg cggacgagca
     2281 ctgcgtcact gccaaacaca cttttccgat gacggttaca accgttgaac gcggattaaa
     2341 agtggcggcg ttcgatggca ccgcgccatt ttttgtgctg agcgacgcgg caaccgcgat
     2401 tcctacgagc aactggtatc gcaatttcga tctcgcggct gagcgagctc gcgggcttcc
     2461 cgatctcacc gaccattttt acgcgggcga actgcgagca acgattgcgc cgggaggttc
     2521 gctgacgatg gtcgcgagca ccagcgccac cgcatcgctg gacggcgagg ccgccttcgc
     2581 gaaacgtcag aaggaagcgg ccgctttgct gaagcatttt tccgccgcga acattgccat
     2641 aacaaaagaa aattcacagc cggcgttaac gtccgtgcag caactcgtac ttgccgcgga
     2701 tcaatttatt gcctcgcgcc ctttggaaga tgcgccaaat gcaaaaacaa ttctggcggg
     2761 atacccatgg ttccatgact ggggacgtga cacgatgatc gcgttaccgg gattgtgcct
     2821 cgtcacggga cgtccctggc tcgcgcggaa tattctgcaa acctattccc gttttgtgag
     2881 cgaaggcatg ctgccgaatc aatttccggg cgcgggcgtc gcgcctgttt acaacagtgt
     2941 ggatgcaacg ctgtggtatt tcgaggcgct gcggcagtat ttcgaagcga cgaacgatat
     3001 tggcttgctc cacgaattat atcctgtgct ggaaggcgcc atcgaagctc accagcgcgg
     3061 cacccggtat cacattcatg tcgatcccgc ggatggctta ctgttcgcgg gcgaagccgg
     3121 tgtgcaactg acctggatgg acgcgaaagt caatggccgt gtcgtgacgc cgcgcatcgg
     3181 aaagccaatc gaggtcaatg cgctgtggct gaacgcgacg gcaacgatgg cgggatttgc
     3241 cggcatcctt ggacggaatg cggcgcggta cgaaacttta aggacaagcg cgaaagcggg
     3301 cttcgcaaaa ttctggaatt ctgaaaaaca attttgcttc gatgtgattg acgcgcccgg
     3361 aagcgtggac ggaaaagatg cagcgctgcg gcccaatcag atttttgctg tttcgctgcc
     3421 ggaaaccgct ttggacgcga agcagcagcg cgcggttgtg gacatttgcg cgagcgagct
     3481 tttaacttcc ttcgggctgc gtagccttgg gcaaaacgaa ccgggatatt gcggccatta
     3541 cggcggcggg atggaggagc gggatgatgc gtatcatcaa ggtacggtgt ggggctggct
     3601 cctgggtcca ttcgctctcg cgcatttgcg tgtattcgga agcgcacagc aagccatgtc
     3661 ttttctggaa ccgttgtttg gccaaattaa atccgcggga ctcggaacgc tgagcgaaat
     3721 ttttgacggc gacccgccgc atattcccaa cggatgtatc gctcaggcat ggacggtcgg
     3781 agaaatgttg cgggcgtgga aaaatattgc ggccgcccgt gaaaacgaag agggcaaacc
     3841 gaggaagtga agcttgggaa tgccgggact tttcatgcgc attcccaagc ctattatttt
     3901 ttctactgaa aaatcaggct agcgacggaa tccgccaaac ctgccatatc cgccataaaa
     3961 tcccaacccg atgactggtg ccggatacca accgtaagga tagccgtacc cgtaaggata
     4021 accataggca tagggatatc cgtaataagg cggtggcccg ggccttctca tctgtggccg
     4081 gttttgtcgc gggcgcgaat ccaagtcctc ttgcgagaca ggttgaaacg cgaattgact
     4141 attgactgtt gaaatggtca ctggattctt aagccggaag gacaacacag tttccggcga
     4201 aatcgtcgtc ggtttgccgg gagtggacat gaccccagcg attcccgcag tcgcgccgat
     4261 tccagcgccg attgccgcgc cggttccgcc ccctgcaata ccgccgatga tggctcccaa
     4321 tccggttgtc gtcccgaccg tcgcaacgtt tcggcccgtt gaggatccgg cggaagtttg
     4381 gaacaattca gtttgaagag gcaattgctg gccgtcgact aacgtcaagt caggcaaatc
     4441 cattccaagc tgcgaattac cgttgccatg tccgcccttt ttgaccaggg agacgcggcc
     4501 cgtctgcgcc tgtcccctac gcgccacaac ccagccgttt acgacgagtg gctggtctag
     4561 aacggcgctg aaactatcgc ctgtcacatt gcggtcggtc gaaatccatt cgtcgacgcg
     4621 gacacgaacc accgttccag caggcaaggt aagggtttca ggaggcaatg gctggttgga
     4681 ctgtccttgc gcttgcccag cgggtcggtc ttgggatggc ggtccttgct ggttctgagc
     4741 cggggaatcc ggaggagcct gcatttgtcc gtcgggcgaa gggtcattcc tctggtgctg
     4801 tacattatct tgatcttgtt gattttgccg cgcatgcaag gctgaagggg caaacgaaag
     4861 acaaaggccc aagcatgcaa cagtcaggtt tttcaggggg atcgaacttc ctttttcttt
     4921 ccaatgggag atcattttct tcttgctcct agtcgctcca cgcctggtag cccccgatag
     4981 ccgctgggcg tttggaataa accatttact ttagtactca gtttgatgat gcgaaatgac
     5041 catggagttg tccggctaat atagcagtgt tccagcggta cacgaattga tagttgcaaa
     5101 ttgttttgga ggagacagta aataagctgg aaataaaaca acaccgcagc aaggaaataa
     5161 tatacgggca cactcattcg gtgacatcaa gcgtgagaag atctgcaaga cgtccgaatt
     5221 gatttcagca ccacggccaa caaaagccca ctgaaacgtg accgccgcga tccatcatcc
     5281 attgcggtga aaaatcgcgg cgcgttcacg agtcgattat gtttttacag ctaggctttt
     5341 actttctttg gctttttctt ttcgcgtttt ggtgcgtttt tgtttcccat tttgatagtg
     5401 tgccatgatt ttttgagctt gaccagagac aatgaaccgc gcaccgcatc gtttcagtga
     5461 gtcgtagcct ttcagtcaac aggtcaggta agatgacggt tccgatacta tcgcagaccg
     5521 tgttccgagg cggaggttcc atgcgtccta aatggcaaat tgctatactt acgatctgcc
     5581 tgttttccgc catattcatt gccgcacgag ctcgttctca atctgtcccg gcggctgcag
     5641 ccgcgcagca gatcgactcc aaacaatcga gttccggcca atctggctac gtcctaaaag
     5701 tgaccacgcg tctcgtgacg ctcgacgtca ttgccacaga ctctcacggg aatccggtgc
     5761 gcgacctcaa gcctgaagat ctgcaaattt tcgaggagca taaagctcag caaaaaatcg
     5821 agcactttga atattttgaa acactcaaac ggcccggaac cgtggaaaat gccgcgagcg
     5881 cgattcgaaa agcttccggc atcatttcca atcaattgcc tctcgaccaa ttgaaaattc
     5941 ctccgactgt tttgctgatg gatagcttga acacgcagac gccgaaccag gcgcagggcc
     6001 gcgcgaaaat gattcaattg ttgagggggc ttccgccgga tacgccagtc gccgtttttt
     6061 tgctgggaag ttcgttgaga attttgcaag ggtttaccag cgatggacga ctcctgcggg
     6121 cagctctgga tcgaagtttg gttgggaatt cgattcagca ggacccgcgg aacgatcttg
     6181 gcgacacttc caactacatg caagatacga ccgccggcac gggctcaaat ctgggactcg
     6241 cggcccagtt gggcgaagtt cagaattttg agaaggagga atattctctg tcggtcgatc
     6301 tacgcatgaa ggagacgctg ggagcgctca ctcagattgg acaatatctc agcggaattc
     6361 ccgggcgcaa aaatctgata tgggtttcgg aatccttccc gcttaccatc gtgcccgacc
     6421 caacaacggg caacaatcca ttcggcggtg gccgggaata ttcagatgaa atcagaattg
     6481 ttggaaatag attgacggac gcgcaagtgg ccgtgtatcc gatggacgtt cgcgggcttc
     6541 aagcacagca atcgctatcc gcctcacaga atgtaagtct tcccgaaaca cggctgacag
     6601 ggcctgcgat tgccgaccga ttgaacgtcg aagatgcgga tttacagcag acgcttggca
     6661 cgatggacga aatcgcgcag gatacaggcg ggaaatcatg caagaacggc aatgatcttt
     6721 cgggatgcgt tatgacggcg ctgaaagaca gctcctctta ttacgaaatg tcgttctatc
     6781 catcgaaagc aaattgggat ggaagatttc acaagattgt ggtgaagact tcgcggtcgg
     6841 gagtgaaact cgtttaccgc cgaggctact atgcgctcga cgcagcatcg ctggcgaagc
     6901 aacaacagcc ctctgatcat ttgcgccagg cctgcgcgga ccaattgcct tctacgacgc
     6961 ttcctttaac cgtacagcca ttgacgccaa gccagtatgg aaatgcgcag ggcggcctgc
     7021 actacatgct tatgatttcc gcggccggac taagcgccac gcaaacaggc gattcctaca
     7081 acctgaatat tcgagtggcg gattgtgaat tcgcgaataa ggccacggta tttcgattca
     7141 acgaaagaga aatgccaaaa acagtttcca gcgaattatt ccggagctgg caatcgacgg
     7201 gaattcccga taacgtcgcg ttgatgccgt cttccgacac acaaagaatc cgtctggtgg
     7261 tgctcgatgt cgcttcgggt ttgaccggcg caatcgatat tcccgtgctt ccttcggaaa
     7321 ttgcgaaagc aaccgcgcca atcgtgcctc ccaagcccgt ggtcacaacg ccttatgtgg
     7381 aatttccgga taaagtgaaa gggccgtcgc agccgaaaat tgtcgaatcc ctgacattta
     7441 gtctcaattc gaacgcgtcc ggcactctga attggaaagg cgacagtctc gtttatcaat
     7501 tggcgggaga tatgccgttg gatagggccg caaatgcgtt ttcaggttac gcattcggcg
     7561 gacgattcca ttgccaggac ggacattttg ttcctgtgga agcgtcggat ggcgacgcgg
     7621 cattgcgttt cacgttccat aatcacagcg gaaagttgct ggtcgtggat ctgaagggcg
     7681 agcagcccgt ttatgtaggc gatcttccgg tcgatcccag tgcgcaaaca ttttttgagc
     7741 aggtttggaa ggcgagccat tgccaggaat aaccggcagg aaccgggcac gattgaaaaa
     7801 tttagaaaac tgtggcgacg cccaacagcg ccaacaaacg cttagtgcga aagagtaggc
     7861 tcctcgagaa tgtcgcgact cggttgattc gcggggggat ttcttcgcag ccacaggctt
     7921 attaatgttg cagcggccgc gcacaatgcg ggaatcgcgc ctgcctggaa aatttctcgc
     7981 ggaccccaat ccagcttcag catcacgcca accagaacgg gccccacaat cgaaccgatg
     8041 cgccccactc caagcgccca gccaatgccc gttgcgcgaa tggaagtagg gtagatcgtt
     8101 gcgccaatca cattcagtcc tccctgcgct gcctgcaccg taaaacccag gacgaaagta
     8161 atcgtcattg ttaacgggaa tgaagccgaa tacgcgagcg aaccgattaa tgcggcgctg
     8221 cacacgaatt cagccaatag aattgtgaaa gcgccccacc ggttaatcaa aactccttcg
     8281 gccgcggagc cgaggatgcc gccgacactg aacagaatga tggccgtgat tcccgctgag
     8341 acaggtaaac cccgttgtcg aagcaaagcg ggcagccaac tcacgacgaa atacagaatc
     8401 aaaagattca taaaataggg aatccagagc aggatggtgc ccgccgcgcg gtcttccgtg
     8461 aacaagcgaa tgacgggtgc gtttttgtgc ttactcagtt taggagaacg agaaaaagga
     8521 gcgtcaccca aatcgggcga aatgcgtttt agtatttttg aaatcttatt ttgattcgct
     8581 ccggagactt ccagatagcg cacggattcg gggagaatgg cgatcagcag caaggccagc
     8641 acgaacggca gaactccccc cgcgtaaaaa accgattgcc atccccagcg cggcagcatg
     8701 accgaactga tcagcccgcc gagcagcgcc cccgcaggca tgccgcaaaa taaggccgag
     8761 acaagaacgg cgaccaggcg tttcggcgcg tactcggaca tcagcgcgac cacgttggac
     8821 agagcaccgc ccaaccccaa tccggtcacg aatcgaattg tccacaactc gggaagcgaa
     8881 gtggcgcggg cggtgagcat cgcaaacgct ccaaatatca gcgtgcaaat cacgaccggc
     8941 cacttgcggc cccagcggtc tgcaatcggt ccaatcagca tggacgaaat catcaggcct
     9001 aaaagagctg ctccaaacac aggcccgaaa ttttttatgg ggacgtgcag agtttcagcc
     9061 attgacggag cgaggaaccc gatactttgc gtatcgaaac catcgagcac aacgaccatg
     9121 gcgcacacag ccatcgtcca gatctgatag cgactcagcg gacgctggtc gatcatttcg
     9181 ctgacggttg ctgcgcgctc catgcaatct cccggaatcc ctgctggatg aattttgtgt
     9241 gagggcagaa tactactgtt tccatgggga atggtagcag tagtcctgca tatcgagtga
     9301 gagcgagaga tttagttatg gaggaacccg caatcttatt ggccgaacaa aaattcagag
     9361 cttcgcgtta ggggatgatg gtgacagctt ccttcatcat gccgaaattg gcgcttcccg
     9421 catccatcga gagaacttgc aattccagtg gccctgcggc ggtggcggga acatccgcgt
     9481 gaaacgtgtt ctgcactccg gcataggtca tcggaatttc cgattgtact ttgccgtcgc
     9541 gcagcaatcg cgcgatcaca tgaatcttgt tcgcgtccca caagccatca ggttccgtcg
     9601 ggcatccgca ggccatcata atagtggctt tcacttcaaa cggcgatccc gctttcacct
     9661 tcgcatccgg cagcggcgaa accgcagtca cgatgaagcc gtgaatttcg agcaaaattc
     9721 cttcgcccag tacatcctcg ccgggaacca ggagaagagt cttcgacgtg cgctgcgtgg
     9781 cttgcgcgtt gccgagcggt ccttcggcag aaatttccac gacggtcggc ttgtcgatgt
     9841 gcagcacggc gagatagccg gatgcgtcgg ggctgttata cacgctcata ccgcgcgtgt
     9901 gaggtgtttt cataatcgta ttcgtgtcgc ctgtcccacc ctgctgcatt ccttgcgcga
     9961 ggatttctcc tgtcgctaca tccttcaccg tgatccgcgc cccgcctacg tgggtgccaa
    10021 tcactttcgc atcgcgggcc accgcgcgaa tcatcacctt ggtttccttg ccactggttt
    10081 gggcaaaaag cgacgaagag aaaagtagaa ccgcacaggt tacagcgctc gataaaattc
    10141 gggatttcat tggcacccct ccatggatgc gacaaaacat atcacatttc ggcgctggtg
    10201 gaggatcgct ggtttgcgcg gcaggagtct cgatccgagt gaccttggcg cgcgggaaaa
    10261 gtgcccgttt cgtttccaag aacggggacg aaacggcctc tacgaaaaac cgtcgaatta
    10321 gtttggcttt cgtggcgacc gcacgaattt tgggcgggcg gctttagatt atccactgct
    10381 aaaaatcact gttaaaaatt gaaattgccc gcttgccttg ttgcaatgcg atgggcacta
    10441 cacatacatg gagtcttaga ccacccgcga aaccggcagc gtttttcacg tttaattccc
    10501 atcgaaccta tcgcaaggtg actattcggt atctctttca ttaagttcat taagtcgctc
    10561 ttaatttgca acgaactcaa atctgttttg ccgcagagaa gcgaatccag ctagctggta
    10621 caattgttcc gtctatttgt gtgcatagga gaagccagca atgaagccgg attcggatcc
    10681 gaatttaaaa aagcccactc ggcgaaaatt tatccgtacc ggcttactcg ccgccgcggc
    10741 cccagctctt gcagtttggc ctgcgaatgc ggttcagaat gcggcacaag aaccttcccc
    10801 acagcaagca tctccggagt ttaccgggcc cggagcaaat ccatattgga attcggttgg
    10861 gccgattgct acatatccgc agaaggctcc actaattctc ttgacggatc gtccagtcga
    10921 actcgaaacg ccgcgccatt attttctttc cacgttcacg ccaaatgaag ctttctttgt
    10981 gcgctggcat ctgcaaacca ttcccaacgc cgtcgattta aaaacttggc gcctgcatgt
    11041 ggaagggaat gtggaacggc cgctcgaatt gagtttcccg gatatgctct ctcgcttcaa
    11101 aagcgtaacc gtcgccgcgg taaatcaatg ctccggcaac agccgcagtc gtctgcagcc
    11161 tcgagttgct ggagggcagt ggggaaatgg tgcgatgggc aatgcgtcgt ggaccggcgt
    11221 aaaacttcgc gagattctca gtgctgccgg aataaagagt ggctcggtgc aactgcaatt
    11281 cgaagggctg gacaagggtt tgggtccgaa gggtttcggt tcgaatcggt ttatgaagtc
    11341 gctcgattta acaaatcctg ttctcgacga atgcatcgtt gcctatgcga tgaatgaaga
    11401 accattgccg gtgctcaacg gttttcctgt acgcctcatc gtccccggct atttcgcgac
    11461 gtattggcta aaatcgctta cgtggatacg cgtcctcgaa actacggatg ataatttctg
    11521 gatgaagggc gcttatcgga ttcctgaaac acctcgcggg aacacaaatc cggaagaaat
    11581 gaaatctggg agtgtaaaaa ccgttcccat cactaaaatg ccagttcgct cctttcttat
    11641 ttcaccggac ggttcgcgga aaattcccgc cgggttcccg ctcaaattgc gcgggattgc
    11701 gttcagcggt cacggcggca ttaagcgggt cgaagtatcc gcggacaacg gatcaaactg
    11761 gagcgatgcc cggctggagc aagatgcggg cgaatattct tttcgcacat ggtcactgga
    11821 gtggacaccg agaagccctg cgcaatacca aatcgccatt cgcgcaaccg acacgaaggg
    11881 aaatgtgcag actgacgaaa gggtttggaa tacaggcgga tatctgtgga atcaaattga
    11941 gcggcaggaa attgtagttg gagaagcgag ttaacaggag ccgtgcgtgc ataaaagaaa
    12001 atggattctc gtcttggcta ttgggctgct tgccatgttg cttggccagg gcatttatgc
    12061 cgattttcga gctggctatt acaatcctgc agggcctgaa accatgcacc aaattctgcc
    12121 tgcctcggct tccacggatt ccacctatac tgccgggcaa tatccgattt attatcctga
    12181 gctcgctgac ggtgacggaa agacggaagt cgagaataat tgcgagttgt gccacagccc
    12241 acgctacatc accatgcagc caccgatgtc agcgcaagcc tgggcagtcg aagtgaacaa
    12301 gatgagaaat atttacggcg catctattcc cgaagccacc gcgcaaaaga ttatcaatta
    12361 ccttggaacg cactacactc cggaaacccg aaagcgttag aataccgggt acgggattgc
    12421 aatcttgccc tgccttggga ggatatccat gattcgagtg aacgccgtcc gcggcattat
    12481 actggccgca ctcacggttt ccgccatcgg ttggttctcc gcaacttcgg taagctatgg
    12541 cgcgccgagg acgccgcagg cagcggacac ttccaaggca tgggtcgcac cgaatccttc
    12601 cacgattccc gccgggccgt taggtgacag tattcggctt ggcatgaacg tttttaacga
    12661 cacgccgaag tatgcggaac gttacgtcgg caataaactg agctgcacgc attgccatat
    12721 cgatggcggc acagtttcgc agggaatgcc catggtcgga ttaccaggaa tatttccgat
    12781 gtatcgggac cgcgaaaaaa ccgtgatcac ttttgaagag cgcattgagc agtgcttcca
    12841 gcgcagcgaa tccggacacc gcattccgaa cgatagtcct gagatggcgg gcctggttgc
    12901 ttatgcccaa tggctgtcca aggatcaagt taccggccgt ccatttcctg gacgcgggct
    12961 cgtcaacctt cctgaactga ctggtgatgc cgcgaacggc agcaaaatat ttggcgagca
    13021 atgcgccgtt tgccatggca cagatggcgc ggggaaactt cccgtggcgc cggcgctttg
    13081 gggaccgaac tcgtatagcg acggagcagg catgaaccag atttccaaga tggcggcgtt
    13141 cgtgcagcac aacatgccgc agaatgcacc tgacacgctg acaccgcaag aagcgtacga
    13201 cgtggcggca tttattcgca gcaagccgca cactgttttt gtgatgaaag accatatgta
    13261 gccggttcgc gcaaactttt cctattttct cgcacgctcg taattgcgac tcaaatccgc
    13321 ggtcaaggat ctgcatgaaa atgaaagtga atttaatgcg tgtggctgtc atgtgctcgg
    13381 gactggccgt tgccatgggc ttgttgtttc ttccatctgc ttcggcgcag gataatgcgc
    13441 caaaatttga agtcgatccc atgtggccca agccgctgcc caatctttgg gtgaccggag
    13501 gtgtcggtgg cctgtgcatt gattcgcacg atcatgtttt tattcttaac cggcgggacc
    13561 tcacggacaa cgatctcgat gccgggcgcc aggcgccggt ggtgattgag ttcgatcccg
    13621 gcggaaacgt tgtgaattca ttcggcgatc caacgacaac gccaaacacg ccgcacggct
    13681 gcacttttga taaagataat aatttatgga ttaccggaac aggcgacggc atcgcgcagg
    13741 agtatacgca cgacggtggc aaactgctcc gccagatcgg aaagatgggc acggtcgatt
    13801 cttccgacgg cacgggcaag gggaaacctc tcaattccag ccacagctca ttttttaaac
    13861 ccgcgggcat tgcgatcgat ccgaaaaacg gcgacgtgta tgtggcagat ggagaaactt
    13921 tgggcggcaa tcaccgcgtc gcagtgttcg atgccgaggg aaagttcttg cgtcagtggg
    13981 atttgagccg caccgaagcc gagactggta acg
//
LOCUS       DASZMB010000009        15183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4954467, whole genome shotgun sequence.
ACCESSION   DASZMB010000009 DASZMB010000000
VERSION     DASZMB010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 15183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15183
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4954467"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1735)
                     /gene="nuoL"
                     /locus_tag="VG272_00225"
     CDS             complement(<1..1735)
                     /gene="nuoL"
                     /locus_tag="VG272_00225"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWF12132.1"
                     /translation="MMPTEYFLVHIWLIPLFPLAGAALMLFIGRRLPNAAVNVICVGS
                     VFLAMCYAFGAIWQLVSRPVSERVVSYALFDWVPAGIMHTNSGRVMDFHVPWGVLMDP
                     LTAVMLFVVTGVGFLIHVYSTGYMAHEGGYYRFFGYLNLFMFSMLTLILANNLLLLFV
                     GWEGVGLCSYLLIGFYFLRKSASDAGKKAFIVNRIGDAGFILGLFLMLGTLGTIQFTE
                     IGPAIAAGHFAMGDASLTAIALLLFVGATGKSAQLPLYVWLPDAMEGPTPVSALIHAA
                     TMVTAGVYMIARTNAVFSMAPKALFVVAVVGCATSIFAASMGLLQNDIKRVLAYSTVS
                     QLGYMFLACGVGAFTAGVFHLMTHAFFKACLFLGSGSVIHAMSGEQDMRKMGGLWGKI
                     PTTAKTFVVASIAISGIPPLAGFFSKDAILGHAFEYNKLLWLVGIITAGMTAFYMFRL
                     VNMTFFGKSHVDHEVEHHIHESPRSMTVPLLILATLSFVGGWVGWPESLGGSDRFAKF
                     LEPVMAKHAEEAGEAAASQAASGASMEYLLMLLSVLVACGGIYLAYRWYIQRPEVPEK
                     IAASSPFLYNLI"
     gene            complement(1850..2152)
                     /gene="nuoK"
                     /locus_tag="VG272_00230"
     CDS             complement(1850..2152)
                     /gene="nuoK"
                     /locus_tag="VG272_00230"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004321.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HWF12133.1"
                     /translation="MIPISHYLFLSMVLFTIGVIGVVTRRNVIIILMSIELMLNAVNI
                     NLVAFSRLVGSAEGHAFAIFIITDAAAEAAVGLGIIIAFFRNRETLLGDEMDLLKW"
     gene            complement(2169..2678)
                     /locus_tag="VG272_00235"
     CDS             complement(2169..2678)
                     /locus_tag="VG272_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWF12134.1"
                     /translation="MMLDQIAFYFFATLAVVSAILVVTRRNVVQSAIFLITALLATAG
                     IFLQLHAEFLFIVQVILYAGGIMVLFVFVIMLINMDVSLHQIQYNRQWWLGLLLALVL
                     GAELAIAIAIGRSHLSFGEAAPASALEPSTQLVGRVLFSEYMLPFEIASLLLLVAMIG
                     AVVMAKKKI"
     gene            complement(2725..4041)
                     /gene="nuoH"
                     /locus_tag="VG272_00240"
     CDS             complement(2725..4041)
                     /gene="nuoH"
                     /locus_tag="VG272_00240"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWF12135.1"
                     /translation="MNVLHFVISIWVPLVCAVIIVAVLPLVAGYIVLMERKVMADMQA
                     RLGPMRVGPHGLLQPIADAVKLLIKEDIIPDEADKAIFWFAPLISVTAAMLAMSALAI
                     GPWFQIAELNVGLLFIVGISALGIFGIVLGGWSSNSHYSLLGALRSAAQLVSYETAAG
                     MAIVGGLLLAGTLSTREIIQAQNDYGTWFAFAAPVGFFIYIVASIAETNRAPFDLPEA
                     ESELVAGYMTEYSGFRWALYFLAEYTNMVVVASIATTLFLGGWLRPFAGTAAFNFLDF
                     FPTLLMFFVGAYCFYRAPKQPVKVQSLFMVAVGLMMFVAGIILAAPLIPAVANMLPGL
                     KPGLHGAFWFLLKVGLYIYLFMWLRFTFPRYRFDQLMRLGWQFLIPLSIVNVMGIGVA
                     LVLHRHWGWSHWTAFGLTTLITLAIAGWLGWAGEKHDTEHVFVGEV"
     gene            complement(4052..5194)
                     /locus_tag="VG272_00245"
     CDS             complement(4052..5194)
                     /locus_tag="VG272_00245"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HWF12136.1"
                     /translation="MPDERIPFATHADHTVLGADEIVLNMGPQHPSTHGVLRVKLRLD
                     GEKVIGSECVIGYLHRGVEKIAEHRSYQQFAPYVDRMDYVAAVSNGLGYCEAVEKLLG
                     AEAPPRARVIRTILTELQRIASHLFWLGTHALDIGALTPLFYCLREREEILKIFEKFC
                     GARLTTHAFRIGGLQYEAYDELEADVERFCKPFDKRVDEYESLLTENRIWVGRTKGIG
                     ILTAKEAIDYGASGPVLRASGVKWDIRKAMPYAAYDQYKFEIPVGKNGDTFDRYIVRM
                     EEMRQSRLICLQAIENIPAGPIMGKVGKVLKPPPGEVYHAIEAPKGELGYYIVSDGTT
                     QPYRVKIRPPSFINLEAFDKMIRGHLVADVVAIIGTLDIVLGEVDR"
     gene            complement(5198..5911)
                     /locus_tag="VG272_00250"
     CDS             complement(5198..5911)
                     /locus_tag="VG272_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWF12137.1"
                     /translation="MANDPEEKQPPANVPPEPSAPKTPAASGEPASKPAEPSVPAAAK
                     PAAPPAPAAAKPTAPRPPAAPPAPKEGPVALDNDLVKRYKEKFGSAILEAWIDRKQTI
                     LVVARELLAEIATYSRDDEKFDWLSDLTAVDWPKREKRFDIVLLMYSMEKNERLRLKV
                     HATAEERVPSVQGIWSTANWMEREAFDMFGVVFEGHPDLKRILLPDEWQGYPLRKDYD
                     ILTQDTAWIRENLGIESGQ"
     gene            complement(6106..6486)
                     /locus_tag="VG272_00255"
     CDS             complement(6106..6486)
                     /locus_tag="VG272_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWF12138.1"
                     /translation="MPTQYIPIFIFAALAAAFPALSLILFKFIRPTPVAAGAKLKPYE
                     CGVDPEGDTRGRYSVRFYIVAILFVIFDVETTFLFPWAVLFSTREQGFRLPEIYALAS
                     MFVFLGILIVGYVWLYRKGALEWV"
     gene            6970..7485
                     /locus_tag="VG272_00260"
     CDS             6970..7485
                     /locus_tag="VG272_00260"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HWF12139.1"
                     /translation="MPGWLPTFVFHFCQWLDQTPFGHEMRESLYLFPVVETLHVFGIV
                     SLVTAAFMLDLRLFGKGPMRDEPVEKLAKWVLPWVIAGFSVQLISGVLMFSAEATRSA
                     LNSFFWFKMFAILAAGINALIFHTTVYKQVASWNNAQVAPLAARVYGTFSVILWLSII
                     TLGRWFSFHLT"
     gene            complement(7586..8029)
                     /locus_tag="VG272_00265"
     CDS             complement(7586..8029)
                     /locus_tag="VG272_00265"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR03562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWF12140.1"
                     /translation="MVRKASAVWNGSLKEGKGTISTDSKVLSNAQYSFSTRFENGIGT
                     NPEELIAAAHAGCFSMALSGQLGNAGMTAESIETTANVTLEKTDAGFTVTKIHLDVTA
                     KIPGADPAAFEKAAQNAKAGCPISRLLKADITMTAKLAGGTAASA"
     gene            complement(8099..8938)
                     /gene="proC"
                     /locus_tag="VG272_00270"
     CDS             complement(8099..8938)
                     /gene="proC"
                     /locus_tag="VG272_00270"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HWF12141.1"
                     /translation="MKMTSSGKLASKRLAVLGSGKIGGILLRAFLEQKLLQPKRVHAT
                     VRHSEKARALAKQFGIRASTDNRAAVRDADIILLAVKPQVVKEVLEEIKPEMKAGKMI
                     VSVVASVTTQLMEKQLGMDLPVIRAMPNTPCAIGCGMTGIAKGVHAGKEHLEIAQSMF
                     EAVGRVVNVDEKLIDAVTGLSASGTAFIYIILESLAEGGVKVGLPRDIATLLAAQTMM
                     GAARMVLDTGDHPALLKDAVTTPAGSTVDGILELEDGKLRVTLIKAVMKATQRAKELI
                     VES"
     gene            complement(9265..9741)
                     /locus_tag="VG272_00275"
     CDS             complement(9265..9741)
                     /locus_tag="VG272_00275"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HWF12142.1"
                     /translation="MRVAILTISDSVSRGTHKDASGPDLLARCAQLNWEVVATEIFPD
                     DPVAIRDRLISLADEGAADLILTTGGTGIGPRDSTPEATTQACQKLLPGIAELMRDEG
                     RKKTPRAVLSRAVAGVRAHTIVVNLPGSPRGAVESLDAIADLLPHALQVLGGARHD"
     gene            complement(9842..10588)
                     /locus_tag="VG272_00280"
     CDS             complement(9842..10588)
                     /locus_tag="VG272_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF12143.1"
                     /translation="MDASSQDVQVLVVDDNPIIRDLITRGMEAHCAVTGSSDGADALL
                     KSVDAPPDLIISDFRMPGLDGRQLYEKLRSRDRTRNIPFIFIASRGDIEEKLRPSVGG
                     GVEDFIAKPFFVADLVRQVKKIVDRLHLEKMQRRASRPGIIQGRLEEMSVTELMQSLE
                     MGQKTCRLTLKHADQKSELYFNAGQCRHAQSGSTEGDTVVYQAIGWLDGEFEIEFNAT
                     SDRETTTLSTTGLLMEAMRLMDETNESVQS"
     gene            complement(10611..11087)
                     /gene="moaC"
                     /locus_tag="VG272_00285"
     CDS             complement(10611..11087)
                     /gene="moaC"
                     /locus_tag="VG272_00285"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006870.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HWF12144.1"
                     /translation="MKKLTHFRKRGEVAMVDVTSKEVTERTAVARGFVRMAPAVVRAL
                     RAQRVPKGNPLEIARIAGIAAAKRTSDWIPLCHPIPLTHVDVTARLCKNGVEIESRAT
                     TAGRTGVEMEALVAVSAAALTVYDMCKALDKGMEITDIVLLEKIGGKSGHYLRKKK"
     gene            complement(11097..12320)
                     /gene="glp"
                     /locus_tag="VG272_00290"
     CDS             complement(11097..12320)
                     /gene="glp"
                     /locus_tag="VG272_00290"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HWF12145.1"
                     /translation="MNSMWSYTDARQKVIEVASALSRPLTLTQVEIEQAFGRILATPV
                     LADRDYPPFDRSTRDGFAVRAADAVAPGARLECVGELRAGGQLDRTVLEGQCVEIMTG
                     AAMPKGADSVVMIEHIKRDGRSITLERAAKKGEHIVPRGTEAREGALLVAPKTRMGYA
                     EMALSAQAGATRLNVFAAPKLAILSTGDEVVDARSKPGPLQVRNSNGISIEILARTAG
                     AETIQLGNAPDEKVALRELIERGLEADILVLSGGVSMGKYDLVEQVLADLGAEFHFTG
                     VSIRPGRPAVFGTCRNKLVFGLPGNPVSTMVTFELFVLPAVDVLNGGTPRPLPVFRAK
                     LATQVREKGPMTHFLPARLEWDGREARVTQLPWQGSGDIVALALANGFLIVSPDRPEI
                     GPGEWVDVIPRRGAV"
     gene            complement(12324..13622)
                     /locus_tag="VG272_00295"
     CDS             complement(12324..13622)
                     /locus_tag="VG272_00295"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF002223.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HWF12146.1"
                     /translation="MKSIVVVGAQWGDEGKGKVVDYLAASFDYIARCAGGHNAGHTVI
                     FNGSKYVLQLIPCGILRPGKHAVIGSGVVVDPAALVVELDTLAKAGIDITGRLHLSNR
                     AHLIFPYHRQMEKAAEVARGAGKIGTTSRGIGPTYEDKMARHGIRVGDLLDAARFREK
                     LARVIADKDAVCRATYGVPLDTVGLAEQYLAYAERLRPLISDSSELINDALDEGRSVL
                     FEGAQGTMLDIDFGTYPYVTSSNAISGGACTGLGVGPTRINAIAGVTKAYTTRVGSGP
                     FPTEMPDLEAKEVRDRGQEYGAVTGRPRRCGWLDLVVLRYAVRLNGISSLVVTKLDVF
                     DTQKEIQVCTGYRLKGTLIKEMPASAEDLAEVKPEYRTLPGWQSDTFGVKDAAKLPRA
                     AVDYLKFISDFLGVEIGMISTGPDRDATIICAGTQLAKWI"
     gene            complement(13928..14110)
                     /locus_tag="VG272_00300"
     CDS             complement(13928..14110)
                     /locus_tag="VG272_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12147.1"
                     /translation="MSERISEEKPQNLDPFQGTIPPALRPARVSLSVGTLSPVALPIP
                     LTTGSIGGRVYFYSSC"
     gene            14177..14962
                     /locus_tag="VG272_00305"
     CDS             14177..14962
                     /locus_tag="VG272_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWF12148.1"
                     /translation="MTSKGMHTPEDLERREFQLSLFAASSIAILAAGLVLLMYPAVYS
                     NRDSSGSRIPQIAFYGFCILSCLLVAYIVDRQLTIHRLRAQIAQERIRTSEALRQASA
                     DVLSAMPNVSTFDDRLAMEFRRAATAESKLTVMVIAIKLNGLFAEPDQSMSALGDAAS
                     AVSRKLREQDSIFILRRTFFGVILPGLGHASIQQVCARISDGLSDVSGASNRFSFKIE
                     TVSYPEQTSSAYDLELAVNGWLPEPDIVPSVRHSASDAVESRK"
BASE COUNT         3510 a         4299 c         4066 g         3308 t
ORIGIN      
        1 aaatcaagtt gtacagaaat ggactggagg ctgcgatttt ttccggaact tcgggccgct
       61 gaatgtacca gcgatacgcc agatagatgc cgccgcacgc aaccagcacg gagagcagca
      121 tcagcaaata ttccatcgac gcgccggatg cggcttgtga agctgccgct tctccagctt
      181 cctcggcgtg cttcgccatc acaggttcga gaaatttcgc gaagcggtcg ctgccgccca
      241 gagattccgg ccagccaacc caaccgccga caaatgaaag cgtggcgaga atcaacagcg
      301 ggacagtcat cgatctggga gattcgtgga tgtggtgctc gacttcgtga tcgacgtgcg
      361 atttgccgaa gaaagtcatg tttacgagcc ggaacatgta gaacgcggtc atgcctgcgg
      421 tgatgatgcc gaccagccaa agtagtttgt tgtactcgaa tgcgtggccc aaaatagcgt
      481 ccttgctgaa aaatcctgcg agcggaggga ttccggaaat cgcaatggac gccacgacaa
      541 atgtttttgc ggtggtcgga atttttcccc agaggccgcc catcttgcgc atgtcctgtt
      601 caccggacat cgcgtgaatc acgctgcccg atccaaggaa taagcaggct ttgaaaaatg
      661 cgtgggtcat caggtgaaac acaccagccg tgaacgcgcc aacgccacac gccaaaaaca
      721 tgtagccgag ctgcgaaaca gtggagtagg ccagcacgcg cttgatgtcg ttctgcaaca
      781 ggcccatgct cgccgcgaag atcgatgtag cgcaccccac cacggccacg acgaaaagcg
      841 ctttcggcgc catcgagaag accgcgttcg tgcgcgcgat catgtacacg ccagccgtga
      901 ccatggtcgc ggcgtgaatc agcgcgctga cgggcgtcgg gccttccatc gcgtccggca
      961 gccaaacgta taacggaagc tgcgccgact tccctgtcgc gcccacaaaa agcagcagcg
     1021 caattgccgt caatgaggca tcgcccattg cgaaatgccc ggcggcaatg gcgggaccga
     1081 tttccgtaaa ctgaattgtg ccgagcgtgc ccagcatcag gaacaggccg agaataaatc
     1141 cggcgtcgcc aattctattg acgatgaacg cttttttccc cgcatccgag gccgactttc
     1201 gcaggaaata aaaaccaatc agaaggtagc tgcacaaccc tacgccttcc cacccaacga
     1261 acaagagcaa caaattattc gccagaatga gcgtgagcat cgagaacatg aacaaattca
     1321 ggtaaccgaa gaagcggtag tagccgcctt cgtgggccat gtaccctgtc gagtacacgt
     1381 gaatcaggaa tccgacgccg gtgacaacga agagcatcac tgccgtgagc gggtccatca
     1441 ggacacccca cggcacatgg aaatccatca cgcgaccgga gttggtgtgc atgattccgg
     1501 cgggaaccca atcaaataaa gcgtagctga cgacgcgttc cgacaccgga cgtgaaacca
     1561 gttgccagat cgcgccaaac gcgtagcaca tcgcgagaaa aacactgccc acgcaaatga
     1621 cgttgacggc cgcgttcgga agcctgcgcc cgataaacaa catcagtgca gcgcccgcca
     1681 aggggaacag cgggatcagc cagatatgca cgagaaaata ttccgtcggc atcataggtt
     1741 tacgtttcta aattctcttg catactacga gctgcaactt aaccgcgaat tctcagtggc
     1801 tagtggttag tggctagcca ctcgtcacta accactagcc actgcctcac taccatttca
     1861 gcaaatccat ctcatcgccc agcagcgtct cgcgattgcg gaagaacgcg atgataatac
     1921 ccagtcccac ggcggcttcc gcagccgcat ccgtgatgat gaaaatggcg aaggcgtggc
     1981 cttccgccga acctaccagg cgcgagaacg ccacgaggtt aatgttcacc gcattcagca
     2041 tcagttcgat ggacatcagg atgatgatca cgttgcggcg cgtcactacg ccaatcacgc
     2101 cgatcgtgaa cagcaccatg ctaagaaata aataatggct gatcggaatc atacttagtg
     2161 tcgcctcatc atattttctt cttggccatc accaccgcgc cgatcattgc cacgagcagc
     2221 agaagcgacg cgatttcaaa cggaagcatg tactcgctaa acaaaactct gcccacaagt
     2281 tgcgtgctcg gttcaagggc gctggccgga gccgcttcgc caaatgacag atgcgaacgc
     2341 ccaatggcga ttgcaatcgc caactccgcg cccagcacca gcgcgaggag aagtcccagc
     2401 caccattgcc ggttgtactg gatctggtgc aatgaaacgt ccatgttgat cagcatgatg
     2461 acgaacacga acagcaccat gatgccgccc gcatagagaa ttacctgcac gatgaacaaa
     2521 aattccgcgt gcagctgcaa gaatattccc gccgtcgcga gcagtgccgt gattaggaaa
     2581 attgcgctct gcaccacgtt gcgccgcgtc accacgagga tcgcggagac cacggccagc
     2641 gtcgcaaaaa agtaaaatgc aatttgatcg agcatcatga tttaaagcgg gctaaaagta
     2701 tcgcccgcaa ccgtttgccg ttctctacac ttcgcccacg aacacgtgct ccgtatcatg
     2761 cttctcgccc gcccagccta gccatcctgc tatcgccaga gtgatgagcg ttgtcaaacc
     2821 aaatgccgtc cagtggctcc atccccagtg ccggtgcagg acgagagcca cgccaattcc
     2881 catcacattc acaatcgaaa gcggaattaa aaattgccag cccaggcgca tcagctgatc
     2941 aaagcggtag cgcgggaacg tgaaccgcag ccacatgaac aaataaatgt acaaaccaac
     3001 tttcagtagg aaccagaacg cgccgtgcaa cccaggtttc aaccccggca gcatgttggc
     3061 gaccgccgga atgagcggcg cggcaagaat aattccggcg acgaacatca tcaagcccac
     3121 agccaccatg aaaaggctct gcacctttac aggctgcttc ggcgcgcgat aaaaacaata
     3181 cgcgcccacg aaaaacatca gcaatgtggg gaaaaaatcc aagaaattaa aagcagcagt
     3241 tccggcaaac ggccgcagcc atccgcccag aaacagcgtc gtggcaatcg acgccacgac
     3301 caccatgttt gtgtattccg cgaggaaata cagtgcccac cggaagccgc tatattccgt
     3361 catgtagccc gctaccaatt ccgattcggc ttccggcaaa tcgaatgggg cgcgattggt
     3421 ttccgcaatg gacgccacaa tgtaaataaa aaatcctaca ggcgcggcga aagcaaacca
     3481 tgttccgtaa tcgttctgcg cctgaataat ttcacgtgtg gacaaagttc ccgccagcag
     3541 caagccgccg acaatcgcca tgcccgccgc ggtttcgtag ctgacgagtt gggccgcgct
     3601 gcgcagagct ccaaggagcg agtagtggct gttcgacgac catccgccca gcacaatccc
     3661 aaaaattccg agcgcgctga tccccacgat aaagagcagc ccaacattca gctcagcgat
     3721 ctggaaccac ggaccaatcg ccagcgcgga cattgcgagc atcgcggccg taaccgaaat
     3781 cagcggcgca aaccagaaaa tagccttatc ggcttcgtcc ggaataatgt cttctttaat
     3841 taagagtttt accgcgtcgg cgatcggctg caacaatccg tgcgggccga cgcgcattgg
     3901 accaagccgc gcctgcatgt ccgccatgac cttgcgctcc atcagcacga tgtatcctgc
     3961 gacgagcggc aaaacagcaa cgatgatcac ggcgcaaacc agcggcaccc aaatggaaat
     4021 cacgaaatgg agtacgttca tcagcatcgc gctaccggtc cacttctccc aaaacaatat
     4081 cgagcgtgcc aatgatggcg acaacgtccg ccaccagatg cccacgaatc attttgtcga
     4141 acgcttccag attaatgaat gacggcggac ggatcttcac gcgataaggc tgtgtcgttc
     4201 cgtcgctcac aatgtagtag cccaattcac ctttgggcgc ttcaatcgcg tgataaactt
     4261 caccaggcgg cggctttaag acttttccca ctttgcccat gatcggcccg gcgggaatat
     4321 tttcgatggc ttgcaaacaa atcaggcgcg actggcgcat ctcttccatg cgcacgatgt
     4381 accggtcaaa cgtgtcgcca ttcttgccaa ctggaatttc aaatttgtat tggtcataag
     4441 cggcgtaagg catcgccttg cggatgtccc acttcacgcc gctcgcgcgc agcacaggcc
     4501 cggaagcacc ataatcaatc gcttctttcg cggtaaggat gccaatgccc ttcgtgcggc
     4561 ccacccagat gcggttttcg gtgagcagcg attcgtactc gtccacgcgc ttgtcgaacg
     4621 gcttgcagaa tcgctcgacg tcagcttcca actcgtcgta agcctcatac tgcaggccgc
     4681 cgatgcggaa ggcatgcgtc gtgaggcgcg cgccgcaaaa tttttcgaaa attttgagaa
     4741 tctcttcgcg ttcacgcagg cagtaaaaca gaggagtgag cgcgccgata tcgagcgcgt
     4801 gcgtgcccag ccagaacaag tggctcgcaa tgcgctgcaa ctcggtgagg atcgttcgaa
     4861 tgacgcgcgc gcgaggcgga gcctcggcgc ccaacaattt ttcgaccgct tcgcagtagc
     4921 ccaagccgtt cgaaaccgcg gccacgtaat ccatgcggtc cacgtagggc gcaaattgct
     4981 ggtaactgcg gtgctcggca attttttcga cgccgcggtg caggtagcca atcacgcact
     5041 ccgagccgat tactttttcg ccgtccaggc gcagcttcac gcgcagcacg ccgtgcgtgg
     5101 acggatgctg cgggcccatg ttcaggacaa tttcgtccgc tccgaggacg gtatgatcgg
     5161 cgtgcgtggc gaatggtatg cgttcgtccg gcattctcta ttgtccgctc tcgatgccca
     5221 gattttcgcg aatccaggcg gtgtcctgcg tcaaaatatc gtagtctttc cgcaacggat
     5281 agccctgcca ctcgtcaggc agcaagatgc gcttcaagtc cgggtggcct tcaaatacga
     5341 cgccgaacat gtcgaacgct tcgcgctcca tccagtttgc ggtcgaccag atgccctgca
     5401 cgctcggcac gcgttcctct gccgtggcat gaaccttcag gcgcaaacgc tcgttctttt
     5461 ccatcgaata catcagcaac acaatgtcaa agcgcttctc gcgtttcggc caatcgacgg
     5521 cggtcagatc gctcagccaa tcgaattttt cgtcgtcgcg gctgtaggtc gcaatctccg
     5581 ctagaagttc gcgcgcgacg accaggatgg tttgtttgcg atcgatccac gcctcgagaa
     5641 tcgccgaccc aaatttctct ttgtatcgct tcacaagatc attatcgaga gcgacagggc
     5701 cttctttagg tgcaggcggc gcggcgggag gacgcggtgc ggtaggcttt gcggcagcgg
     5761 gcgcaggagg cgcagccggt tttgcagcgg cgggaacaga tggctcagcg ggtttagatg
     5821 cgggttctcc gctggcggct ggcgtcttcg gtgctgacgg ctcaggcggt acattcgcgg
     5881 gcggttgctt ctcttccggg tcgtttgcca tgaattaggg gtagaccgat tgcacggcca
     5941 ccgtgcaacc cataataagt tgtagttttt agcacacggc ggaaaacaga gtcaacctgt
     6001 tccgccggaa ttcacgggct tactgaccgt gtaccgcttt cacccaaaaa aaatcccggt
     6061 caccttccgt ggccgggaca ccgtaatcag aacactcggg cgcgcctaca cccattcaag
     6121 ggcgccctta cggtagagcc agacgtaacc aacaatcaaa atccctagaa aaacaaacat
     6181 gctcgccaag gcataaattt caggcaaacg gaatccctgc tccctggttg aaaaaagcac
     6241 ggcccaggga aaaaggaaag tcgtttcgac gtcgaaaatc acaaacagaa ttgcgacaat
     6301 gtaaaaccgc acggaatacc ggccgcgcgt atcgccttcg ggatccacac cgcactcata
     6361 cggcttgagc ttcgcgcccg cagccacagg ggtaggccgg atgaatttaa aaagaatcaa
     6421 ggacagcgcc ggaaaggcgg cagcaagggc ggcaaaaata aaaatcggta tgtattgcgt
     6481 aggcatacct gtatcgccgg aaatcctaac acacgagtca agagagggct actccccgtt
     6541 gattttttgt tgaattaatt tttgttgaca catcgaattt aattgcggag ttaaatttta
     6601 atttaattgc aggcgcagac aaaaattgca gatccaaaag aagggggcct ttacgaaagc
     6661 aagctccaca catttgagtc gcacatgcag aaaagctgcc cgtccaagaa cgggacggcc
     6721 tctacgaaag cagggtggtt tcgtagtgga gtcattctgg gattctaagt ttcaagcctg
     6781 attttccatc ctgcggtgac gtgtgacaaa gatttcatct tggtgtaacc tgaaccatta
     6841 cgtcagagtc taagaaattg atacatcgga gcggcattct cttctgatga aggaaatgca
     6901 cctcgcattg cgatgttgta tcaggttgaa ttgacgattt gggcagatca aatttcaagg
     6961 ggtgaagaaa tgccagggtg gttgccaaca ttcgtatttc atttttgcca gtggctggac
     7021 caaactccgt tcggccatga aatgcgagaa tccctctact tgtttcctgt ggttgaaact
     7081 ctacatgttt ttggaattgt atccctcgta acagccgcgt tcatgctgga tttgcggctc
     7141 tttggtaagg ggccgatgag ggatgagccg gttgaaaaac tggcgaaatg ggttttgccc
     7201 tgggtgatcg cgggattttc agtgcagttg atttccggtg tgctaatgtt ttccgcggaa
     7261 gcgacacgat cggcattgaa ctcgtttttt tggtttaaga tgtttgccat cctggcggca
     7321 ggaattaacg ccctgatttt tcacaccacg gtctacaagc aagtagcgtc atggaacaac
     7381 gcgcaggttg cgccgctcgc cgcgcgcgtc tatggaacat tttcagtaat tttgtggctg
     7441 agtattatta cactgggacg gtggttttcg ttccacctta cgtagatcgc gctttttgcg
     7501 ggtttttcat ggcgccggcc atcgccgacg ccatgaaaga aatttttcct gccaagctaa
     7561 aactttttcg ccacaattta gagaactagg cgctggctgc ggttccgccg gccagcttgg
     7621 ccgtcatggt gatatcggcc ttgagcaggc gggaaatcgg gcaaccggcc ttggcattct
     7681 gcgccgcttt ttcaaaggcg gcaggatccg cgcccggaat tttcgcggtc acatccagat
     7741 gaatcttggt aaccgtgaag cctgcatcgg tcttttcgag tgtgacgttt gcggttgttt
     7801 caatcgattc cgcggtcatc ccagcgttgc cgagttgtcc ggaaagcgcc atgctaaagc
     7861 agcctgcgtg cgccgccgcg atcagttctt cggggttcgt gccaatgcca ttttcgaagc
     7921 gcgtcgaaaa cgaatactgc gcgttcgaca aaactttgct gtccgtggag attgttccct
     7981 ttccttcctt gagcgaaccg ttccagactg ccgacgcttt gcgtaccatg tttgtttcct
     8041 ccgtttaatt tgcaacccaa attttatttc tgtaatgttc cgtgctcaca cctaaagact
     8101 aagattcaac gattaattcc ttggcgcgct gcgttgcctt catgacggcc ttgattagtg
     8161 tgacgcgcag ctttccgtct tcgagttcaa gaatgccgtc gaccgtggat cccgcaggcg
     8221 tcgtaaccgc gtctttcagc aacgccggat gatcgcctgt atcgaggacc attcgcgcag
     8281 cgcccatcat cgtctgcgcc gcgagcaacg tggcaatgtc gcgaggcaga cccactttta
     8341 cgccgccttc ggcgagcgat tcgagaatga tgtagatgaa cgcggtaccg cttgcggaaa
     8401 gtccggtcac ggcgtcgatc agtttttcgt ccacgtttac gacgcggccc acggcttcaa
     8461 acatggactg cgcgatttcc aggtgctcct taccggcgtg tacgcccttg gcgatgccgg
     8521 tcatgccgca tccgattgca caaggcgtgt tgggcatggc acgaatcact ggcaagtcca
     8581 tgcccagttg cttttccata agttgcgtcg tgaccgatgc gacgaccgag acaatcatct
     8641 tcccggcctt catctcaggc ttgatttctt ccaaaacttc tttcaccacc tgcggtttca
     8701 cggctagcaa aataatatcc gcgtcacgga ccgctgcacg attgtcagtg gaagcccgaa
     8761 tgccgaactg tttcgcaagc gcgcgggcct tttccgagtg gcgcaccgtg gcgtgcacgc
     8821 gcttcggctg caacagtttc tgctcaagaa aagcgcgcag caatattccg ccgatctttc
     8881 ccgagccgag cacggccagc cgtttactag cgagtttgcc cgaagaggtc attttcatgg
     8941 ttcaagcatt gttgacgagc ctgaagcgaa cgtcaaccca gttccccaat taatacctgg
     9001 aaagcgatct gtagctcgcc tctcttgccc tgagagaacg cgaaggggca gaggtgaggg
     9061 tttaaatgac agggtggcgc aaaagtccgc ggatccgcgg tcgtgcggca aattcaaggc
     9121 tactttcccg acattcagcg acaaatttct cgctttgagt tggacatcat ctaacccccg
     9181 cctctgaaga ggcgggctac agcagccggt tacaacatcg cgctatatgg cacggttttt
     9241 gtggtgcttt tttattgaga aaattcaatc gtgacgcgcg ccacccagaa cctgcaaggc
     9301 gtgcggcagc aaatccgcga ttgcgtcgag cgattccact gcgccgcgcg ggctccccgg
     9361 caggttcact acgatcgtgt gggcacgaac gcctgcgacc gcgcgggata gcacggctcg
     9421 cggcgttttt ttccggcctt cgtcgcgcat cagttccgcg attccgggga gtagtttttg
     9481 gcaggcttgc gtggtggctt caggagtgga atcgcgcggg ccgatccctg tgccccctgt
     9541 tgtcaggatc aaatccgccg cgccctcgtc tgccagggag atgaggcggt cacgaattgc
     9601 cacggggtca tcaggaaaaa tttccgtggc gacgacttcc cagttcagtt gcgcacagcg
     9661 cgcaagtaaa tcggggcccg acgcatcttt gtgcgtgcct cgtgagacgg aatcgctgat
     9721 ggtgaggatg gctacgcgca tgcaagattc ggtttggaat tttgtggcat aggcttcagc
     9781 ctgtggggtt taagtcttgc acggtctaaa ttccacaggc tgaagcctat gccacatgaa
     9841 actaactctg cacgctttcg ttggtttcgt ccatcaggcg catggcttcc atcaagaggc
     9901 ctgtggtgga aagtgttgtg gtttcgcggt cggaagttgc attgaattca atttcaaatt
     9961 cgccgtcgag ccaaccgatt gcctgataca caaccgtatc accttccgtg cttcccgatt
    10021 gagcgtgcct gcattgacct gcattgaaat atagctcgga tttctgatcg gcatgtttca
    10081 gcgtcagccg gcaggttttt tgtcccatct cgagcgattg catcaactct gtaacgctca
    10141 tttcctccag ccgcccctgg atgattccgg ggcgagaggc acggcgctgc atcttttcaa
    10201 ggtgcaggcg atccacaatt tttttcacct ggcgcaccaa atccgctacg aaaaaaggtt
    10261 tggcgataaa atcttccacg ccgccgccga cagaggggcg caatttttct tcgatatcgc
    10321 cgcggctggc gatgaagatg aatggaatat ttcgcgtgcg atcgcggctg cgtaattttt
    10381 cgtacagctg ccggccgtca aggcccggca tccggaaatc agaaattatg aggtcaggag
    10441 gggcatcgac ggatttgagc aacgcgtcag caccatcgct cgatccagtg acagcacaat
    10501 gcgcttccat tccacgagtg atcagatcgc ggatgatcgg attgtcatca acgactaaaa
    10561 cttgcacgtc ttgggatgag gcatccatcg tgaaacttca ctaagaaaca ttactttttc
    10621 ttacgcagat agtgaccgct cttgccgccg attttttcga gcaagacgat gtccgtaatt
    10681 tccatgccct tgtccagggc cttgcacatg tcataaaccg tgagcgctgc ggctgaaact
    10741 gcgacgaggg cttccatttc aactccggtt cgccctgccg tcgtagcgcg cgattcgatc
    10801 tcaacgccat tcttgcacag gcgtgcggtc acatcaacgt gcgttaaagg aatgggatga
    10861 caaagaggta tccagtccga ggttcgctta gcggctgcaa ttccagcaat tcgagcaatt
    10921 tccagcggat tccccttggg aaccctctgt gcacgcaggg cacgtacaac ggctggggcc
    10981 atgcgtacga aaccgcgtgc cacggctgtt ctctcggtga cctccttcga agtcacgtcg
    11041 accatggcta cttcaccacg ttttcgaaaa tgagtgagtt ttttcacggc gcaccgttag
    11101 acggcccctc ttcggggaat cacatccacc cattcgccgg ggcctatttc ggggcggtct
    11161 ggagagacga tcaggaaacc gttcgccagg gccagggcga caatatctcc agagccctgc
    11221 catgggagtt gagtaacgcg tgcttccctg ccatcccact cgagacgcgc aggcagaaaa
    11281 tgagtcatgg gacctttttc acgcacctgc gtggctagct tcgctcgaaa aacgggtaaa
    11341 ggtcgaggcg tcccgccgtt caatacatcg actgcgggga gaacaaatag ttcgaacgta
    11401 accatggtag aaacgggatt gccgggcagg ccgaatacaa gtttattgcg gcatgtgcca
    11461 aaaacagcgg gccgccccgg acggatcgat actccagtga aatggaattc cgcgccaagg
    11521 tcggcaagga cctgttcgac cagatcgtac ttgcccatgg atacgccgcc cgaaaggacg
    11581 aggatatctg cttcgaggcc gcgctctatt aactcccgaa gggctacttt ttcatcaggg
    11641 gcatttccga gttgaatggt ttccgcgccc gccgtgcgcg ccagaatttc aatggagatg
    11701 ccattgctgt tgcggacctg cagcgggccg ggtttcgaac gcgcatcgac gacttcgtcg
    11761 cccgtggaaa ggatggctag ctttggcgcg gcaaaaacat tcaggcgtgt cgcgcccgcc
    11821 tgcgccgaaa gagccatctc cgcgtagccc atgcgcgttt tgggcgcgac tagcaatgcc
    11881 ccctcccgcg cttccgtgcc gcgaggaaca atatgctcgc ctttcttcgc ggcgcgttcc
    11941 agcgtaatgc ttcggccatc gcgcttaatg tgctcgatca tgacgacgga atccgcgcct
    12001 ttgggcatag ccgcgccggt catgatttca acgcattgac cttcgagcac ggttcggtcg
    12061 agttggccgc cagctcgcag ttcacccaca cattcgagtc gtgcgccggg cgcaaccgca
    12121 tccgcggcgc ggacagcgaa accgtcgcgc gtggagcgat cgaagggagg atagtcgcga
    12181 tccgcgagga cgggagtagc gagaatgcga ccgaacgcct gctcaatttc aacctgggtc
    12241 agggtgagtg ggcgcgaaag agctgaagcg acctcaatta ctttttggcg tgcgtctgta
    12301 tacgaccaca tggaattcat gaattaaatc cacttggcga gctgcgtacc tgcgcaaatg
    12361 atcgtagcat cgcggtccgg gcccgtcgaa atcatgccga tttcgacacc cagaaaatca
    12421 gagatgaatt tcaagtaatc gactgcggcg cgcggaagtt tcgcggcatc cttgacacca
    12481 aatgtgtccg actgccatcc cggcagcgta cgatattcag gcttcacttc ggccaagtct
    12541 tcagcggatg caggcatttc cttaatcaag gtacccttga ggcggtagcc ggtgcagact
    12601 tgaatttctt tctgtgtgtc aaaaacgtcg agcttggtga ccacgagaga agaaatgccg
    12661 ttcaagcgta cggcgtaccg cagcacaacc aggtccagcc agccgcagcg ccgcgggcgt
    12721 cccgtcacag caccatactc ttgtccacga tcgcgaactt ccttggcttc gaggtcaggc
    12781 atctccgtgg ggaaaggacc gctgccgaca cgcgtggtgt aagccttggt caccccggcg
    12841 atggcattga ttcgcgtggg gcccacgcca aggcccgtgc aagcacctcc ggaaattgcg
    12901 ttcgacgacg tgacgtatgg atacgttccg aaatcgatgt ccagcatggt gccctgcgcg
    12961 ccttcgaaca atacggagcg tccctcatcg agcgcgtcat tgatcaactc cgaagaatcg
    13021 gaaatgagcg ggcggagtcg ctctgcgtag gcaagatatt gctcagccag acccaccgtg
    13081 tcgagcggca cgccgtaggt tgcgcggcag acggcatctt tgtcggcaat cacgcgggca
    13141 agtttttccc tgaatcgagc ggcatccagc aggtcgccga cgcgaattcc atgccttgcc
    13201 attttgtctt cgtaggtcgg gccgattccg cgcgaggtgg tgccaatttt cccagcgccc
    13261 cgggctactt cggcagcttt ttccatttgg cggtgatacg ggaaaattaa gtgcgcgcga
    13321 ttggataggt gcagacggcc tgtaatgtcg attccggctt tcgccagggt gtccaactcg
    13381 acgacgaggg ccgccgggtc aacgaccacg cccgatccga tcacggcgtg cttgccggga
    13441 cgcaaaattc cgcatggaat aagctggaga acatatttgc ttccattaaa aatgacggta
    13501 tggccggcat tgtggcctcc cgcgcatcgc gcgatgtaat cgaacgacgc agcaagatag
    13561 tccaccacct tgcccttccc ttcatcgccc cactgtgcac ccaccactac aatcgacttc
    13621 atcggctaaa ggctcccgct tgaattcgcg tccctcacaa aaatgcaaaa aataattaga
    13681 agccatctgg ctctgttggc atccccggtt ccactgtcga tctgctgtag cccgcctgtt
    13741 cagatgcggg ttttttgacg ctccagcatc taaaccctcg cctctgaaga ggcgagctac
    13801 aattccgcgc cacaaaacgg acgaaacttt ctgaagcatc caggatgtca acagagccag
    13861 atgccttcta ctaatctcag cacaccggtt cagtcacacg cctccacccc actccaaaac
    13921 ttcggattca gcaggaggaa taaaaataaa cgcggccccc tatgctacct gtggtcaggg
    13981 gaataggcaa ggccacggga gagagtgtcc cgaccgaaag gctcacgcga gctggacgga
    14041 gagcaggcgg aatcgtgcct tgaaatggat cgagattttg cggtttttct tcggatatcc
    14101 tttcggacat ttagaaactc gttattccac cgtttcgtac ccaattcgat ataattttct
    14161 cgctggtaaa atccacatga caagcaaggg aatgcacaca cctgaagatt tagagcggcg
    14221 ggagtttcaa ttaagcttgt ttgccgcatc gtcaatcgca attctggcag cgggcctggt
    14281 cctgttaatg tacccggcgg tttactcaaa ccgcgacagt tccggatcca ggattccgca
    14341 aattgctttt tatggattct gcattctttc ttgtcttctg gtggcttaca ttgttgaccg
    14401 gcaacttacg atccatcgcc ttagagccca gattgcccag gaacgtatac gaacctccga
    14461 ggctttacgg caagcaagtg ccgatgtcct ttccgccatg ccgaatgtca gcacgtttga
    14521 tgatcgcctc gcgatggagt ttcggcgcgc ggctaccgcg gaatcaaagc ttaccgtgat
    14581 ggtaatcgct attaaattga atgggctttt cgccgagcca gaccagtcga tgtccgcact
    14641 gggagatgcg gccagcgcgg tttcgcggaa acttcgcgaa caggattcga tttttattct
    14701 aaggcgaaca ttttttggag tgattcttcc gggcctggga cacgccagta ttcagcaggt
    14761 atgtgcacgt atttccgatg gattgtccga tgtgtcaggg gcgagcaatc gcttttcgtt
    14821 taagattgag acagtcagtt atccggagca aacatccagc gcatatgatt tggaattggc
    14881 agtaaacgga tggctacccg agccggacat cgtcccgtcg gtcaggcata gcgcgtctga
    14941 cgcggtggaa agccgaaaat aagacgcggc aaaaatgcca gccaaaaatt ttcgagtccg
    15001 tgatgctcag cccgggccct ttgcgtgctt cgtcgtagat ggcgagcttt ttatagcaaa
    15061 cggaaataaa atgctcgcgg caaaacgcta gtccatcaac agagccttct gattcgtgaa
    15121 cacatccggc aaggctgcat tggtcaacta aagtcataac tcttcgtggc cgccaacgtg
    15181 cct
//
LOCUS       DASZMB010000010         6720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3580932, whole genome shotgun sequence.
ACCESSION   DASZMB010000010 DASZMB010000000
VERSION     DASZMB010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3580932"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1313
                     /locus_tag="VG272_00310"
     CDS             72..1313
                     /locus_tag="VG272_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF12149.1"
                     /translation="MKLQTRHFREPIGVALETLRTHKMRSFLMLLGIVLSVSTLIMVV
                     SLISGVNLYVANRIANLGANVFLVLRFPIITDTDEFVKATRRNKPLTWDDYIFLRDNS
                     KLALRVGAQAYQLGRTKANGQTVEDTTVYGVTANIGDMKTEGPASGRYITDSDNQHRS
                     LVALIGSDIGEKLFPGADPIGKEIDVDGRPYEVVGVGKPIGTVLGQTQDNYVYIPIET
                     WMKYYVTSKTSLTINVQTRGSDWMSRTQEEFQTLMRSRRRLGPNDLDNFGILDGGSLV
                     DLFKRITGALAASMVGIVAVFLVIGGVVIMNVMLATVTERTREIGIRKSLGARRGDIL
                     LQFLTETAVMSTIGGIIGVAFAYIFAMIIAANTPVPMHVPILAVIVALTVSTAVGIFF
                     GLYPANKAANLSPIEALRQEV"
     gene            1331..2590
                     /locus_tag="VG272_00315"
     CDS             1331..2590
                     /locus_tag="VG272_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF12150.1"
                     /translation="MQIALGENAKMALDTLQENKLRSFLTVLGVVIGITALITVASIL
                     VGFYADVTVYLEDYGVNTVWIFKVAPGISTGRMTAEERNRKPLTYEDAQAIREECPAV
                     RELSVSIMKRIYDMNRQPEVTARYKDKEVSGLNYSGSDIYYPDVYNFRLYSGRYFTDA
                     EDVHREDVAVIGFDLGKAFFPDGNALDKIIYVEGFPYRVIGVFDHRKGQLFQNPPAEK
                     QVLVPYHTYRKHHPADDEHFIGAMAAPGRMAEAKDEIRAVLRRTRRVPYSAPDNFGLS
                     SAEEIASQFKQITGSVALLTAVVSSIGLLVGGVGVMNIMLMSVTQRTREIGVRKAIGA
                     RRRDVIWQFLTEAVVLTGVGGVIGVVLGIVISLLIHIFIPKLPSSVPLWAVLLGVLVS
                     MSVGLFFGIYPAVKAARLDPVDALRYE"
     gene            2613..3338
                     /locus_tag="VG272_00320"
     CDS             2613..3338
                     /locus_tag="VG272_00320"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HWF12151.1"
                     /translation="MNKINSPRKVKRQIIAMGGGSFGATSEKPVLELYALSQTRKSNP
                     SICFLPTATGDAPTYIAKFYATFAKQRCRPTHISFFERTPRLKEILLAQDAIYVGGGN
                     TKSMLAVWREWKLPQILRQAWNSGIVLAGVSAGAICWFDMGITDSWAEHLAPLPCLGW
                     LPGTCCPHYDGEVERRPAVQQFVAKGKVPQTLALDDGAAAHFVGRKLKRIVSSRPEAG
                     AYLVRQVGQKSVETALPVSYLKK"
     gene            3406..3735
                     /gene="cyaY"
                     /locus_tag="VG272_00325"
     CDS             3406..3735
                     /gene="cyaY"
                     /locus_tag="VG272_00325"
                     /inference="COORDINATES: protein motif:HMM:TIGR03421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HWF12152.1"
                     /translation="MDEQVFQKRCDAAFESLKRRLQEVGDERGFEVEGGSGKLEIQFE
                     DENDTRFVISPNTPVRQIWISALTTSFKLSWSDAANTFVLDKTGETLMQLMGRILTEQ
                     LGSPVTV"
     gene            3741..4394
                     /locus_tag="VG272_00330"
     CDS             3741..4394
                     /locus_tag="VG272_00330"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HWF12153.1"
                     /translation="MISIHRRISQILSPRIKSAQQFEAKFISRLPALFFLLAVAVISI
                     DASAQDNPFPAAASVLQPQGYVSLQPVPRGHAFQVAVVAKISPGFHINAHQPSEDYLI
                     PTKVTAELPAGLFVVETNYPRGVMRAFRFSKTPLRVYEGSFTVLMKLRANGSAPLGPQ
                     KINLTVGYQACNQDACLPPTKIPLTADLEIAAADTPAKPAHTDIFSSSSAEKSPPKP"
     gene            complement(4434..5924)
                     /gene="gltX"
                     /locus_tag="VG272_00335"
     CDS             complement(4434..5924)
                     /gene="gltX"
                     /locus_tag="VG272_00335"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HWF12154.1"
                     /translation="MSTTSVASGNPAAKIRVRFAPSPTGYLHVGGARTALFNWLFARS
                     RSGTMVLRIEDTDVERNRPELVEGILDGLRWLGVDWDEGPYFQSQRLGLHREAAIKLA
                     TSGAAYYCDCPPEKYAGGDAVEETEDVKKEPGPRRITRCACREGKPGLSAENAAIRFR
                     VPVGGVTRFEDAVFGSREVSNDEIEDFVVLRSPKDGQTHGGPTYQLSVVVDDIDMGIT
                     HVIRGSDHISNTPKQALLYLALGATPPTFAHVPLILGPDRTRLSKRHGATSVGSYRDE
                     GFLPEAFRNFLALLGWSIGDAEFMRTTEIIEKFSLEGVSRTNAVFDRPKLEWFNVQYL
                     QKLPIDELLPYIQGELERAKLWQSEWAGEKREWFSKTVDLLRPRTRLLPDFVTWARAF
                     FSDTFEIDPAAREKFWKDERLGPMLDKLADALAPLPDWNHDACDHALRELAAAEGVKA
                     GLLINATRVAIVGQAVAPPLFETMLVLGRDRVVTRLRRAVEILAAH"
     gene            6326..>6720
                     /locus_tag="VG272_00340"
     CDS             6326..>6720
                     /locus_tag="VG272_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12155.1"
                     /translation="MTSESILAITLLAASALTSGFFAYMHSLRRQAYLMLWSAGWCLL
                     ALHYLSPALEPWVSVAPWQASVNEWLLAAAALLFLSTAQVYTNRVPWVHPQIAAGCVF
                     AVWSAANYLNVTKISPQLGVAAVLALVAW"
BASE COUNT         1522 a         1835 c         1779 g         1584 t
ORIGIN      
        1 aaagttcccc accgatccgt gctttgctga cggatgctcg tatgaaagaa tagaagtccg
       61 gcccaacacg catgaaactt cagacacggc attttcgtga acccatagga gtagcgctgg
      121 aaacgctgcg cacgcacaag atgcgttcgt tcctgatgct gctggggatc gtgctttccg
      181 tatccactct gatcatggtc gtttcgctaa tttccggcgt aaatctgtat gtggccaatc
      241 gcattgccaa tctcggcgcg aatgtttttc tggtgctgcg atttccaatc atcacggaca
      301 cggacgaatt tgtgaaagcc acacgccgaa acaagccgct tacttgggat gactatatat
      361 tcttgcgtga caattcaaag ctcgcactgc gagttggcgc tcaggcctat cagctcgggc
      421 gcaccaaagc gaatggacaa acggtcgaag acacgaccgt ctacggtgtg acggcaaata
      481 ttggtgacat gaaaaccgag gggcctgcct ccggacgata tattacggac tcggataatc
      541 agcatcgctc ccttgtcgcg ctgatcggct ccgacattgg agaaaaactt tttcctggcg
      601 ccgacccgat cggaaaagaa atcgacgtgg atggcagacc ctatgaagta gttggagtcg
      661 gaaaaccgat cggcacggtg ctgggacaaa cgcaggacaa ttacgtttat attccaatcg
      721 aaacctggat gaaatactac gtcacgagca aaaccagcct gacgattaat gtgcaaacgc
      781 gtggctcgga ttggatgtcg cgcacgcagg aagaattcca aacgttgatg cgttcccgac
      841 gtcgcctggg cccgaatgat ctggataatt tcggcattct ggatggcggg tcgctggtcg
      901 atttgttcaa gcgaattacg ggcgcgctgg ccgcgtccat ggtagggatt gttgcggtat
      961 ttctggtgat tggcggcgtg gtgatcatga acgtcatgct tgctactgtg acggaacgga
     1021 cacgcgaaat cggaattcgc aaatccctgg gcgcgcggcg cggtgacatc cttttgcaat
     1081 tccttacgga aactgccgtt atgtccacga ttggcgggat cattggcgtg gcattcgcct
     1141 acatttttgc aatgatcatt gcagcgaata cgcctgtgcc gatgcacgtt ccgattttgg
     1201 cggtgatcgt tgcgctcacg gtttccacgg ctgtggggat tttctttgga ctttatccgg
     1261 caaacaaagc cgcgaatctc agcccaattg aagcactacg gcaggaagtt tagattcgcg
     1321 tcgggaaaac atgcaaatag cgctcggcga aaacgcgaag atggctctcg acacgctgca
     1381 agaaaataaa ctgcgttcct tcctcaccgt tctgggcgta gtcatcggaa ttaccgcgct
     1441 catcactgtt gcgtcgattc tggtcggttt ctatgctgac gtcaccgtct acctggaaga
     1501 ctacggtgta aacacggttt ggatttttaa agtggctccg ggaatcagca caggacgaat
     1561 gaccgccgaa gaacgcaacc gtaaaccact gacttacgaa gatgcacagg cgattcgtga
     1621 agaatgtccg gcagtgaggg aactttccgt gtcgatcatg aaacgcattt atgacatgaa
     1681 tcgacagccc gaagtcaccg cgcgttataa ggacaaggaa gtcagcggcc tgaattattc
     1741 agggtcggac atctactatc ctgacgttta caatttcagg ctctatagcg gccgctactt
     1801 caccgatgcc gaagacgtgc accgcgaaga tgtcgccgta attggttttg accttggcaa
     1861 agcttttttt ccggacggga acgcgctgga taaaattatt tacgtggagg gatttcccta
     1921 tcgcgttatc ggcgtattcg atcatcgcaa aggtcaatta ttccagaatc ctccggccga
     1981 aaagcaggtg ctcgttccct accacaccta tcgcaagcac catcccgcgg atgacgaaca
     2041 ttttataggc gcgatggctg ccccaggaag aatggcggaa gccaaggatg aaattcgtgc
     2101 tgttctgcga cgcacgcgcc gcgttcccta ctccgctccg gataatttcg gcctctcctc
     2161 ggccgaagaa attgccagtc aattcaaaca aatcactggt tccgtggcgc tcttgacggc
     2221 cgttgtcagc tccattggct tgctcgttgg aggagtaggc gtgatgaaca tcatgctgat
     2281 gtccgtcaca cagcgcacgc gcgaaattgg cgtgcgaaaa gcgattggcg cgcggcggcg
     2341 cgatgtgatc tggcaatttc tgactgaagc agtcgtgctc acgggcgtgg gcggcgtcat
     2401 cggcgtagtg ctggggattg ttatcagctt gctgatccat atctttattc ctaaattgcc
     2461 gtcctccgtg ccgctatggg cggttcttct cggagttctc gtttccatga gcgtgggact
     2521 tttctttggg atttatcccg cagttaaagc ggctcgactc gatccggtcg acgcgctgcg
     2581 ttacgagtaa attaaatttt taaaacttgg acatgaataa aataaacagc ccgcgaaaag
     2641 taaaacggca gatcatcgcg atgggtggag gcagttttgg cgccacgtcc gagaagcccg
     2701 tgctcgagct ttatgcgctc tcgcagactc gcaaatccaa tccctccatt tgctttctgc
     2761 ccacggccac gggcgacgcg ccaacttata tcgcaaaatt ttatgcaaca ttcgcaaagc
     2821 agcgttgccg ccccacgcac atttcttttt tcgagcgcac accgcgcttg aaagaaatcc
     2881 ttctcgcaca agacgcgatc tacgtgggcg gcggaaacac aaaaagcatg ctggccgttt
     2941 ggcgcgaatg gaagctgccg caaattcttc ggcaagcgtg gaattctggc attgtcctcg
     3001 caggagtcag cgcgggcgcg atttgttggt tcgacatggg gatcaccgat tcatgggcgg
     3061 agcacctcgc gccgctgccg tgtctcggat ggcttcctgg aacttgctgt ccgcattacg
     3121 atggcgaggt ggaacgtcgc cctgctgtgc agcagttcgt ggcgaaaggc aaagtgccac
     3181 aaacacttgc gctggatgat ggggccgcag cccacttcgt gggccgaaaa cttaagcgca
     3241 tcgtaagttc acggcctgag gcgggcgcat atcttgtccg tcaagtgggc caaaagtccg
     3301 tggaaacggc gctgccagtt tcttacctga agaaatagac aaatttcaca tggctacccg
     3361 cggtccttgc tcgcgcgagc gtttcactgc taatctgttt ctgccatgga tgagcaagta
     3421 tttcaaaaac gttgcgatgc ggcttttgaa tctttgaagc ggcgcctgca ggaagtgggc
     3481 gacgagcgcg gatttgaagt cgagggtggg agcgggaaac tcgagatcca gttcgaggac
     3541 gaaaatgata ctcgctttgt catcagcccc aatacgccag tacgccagat ttggatttcc
     3601 gctctgacca ccagcttcaa actttcctgg tccgacgcgg ccaacacttt cgttctggac
     3661 aagacaggcg aaacactgat gcagctcatg gggcgcattc ttaccgaaca actcggcagc
     3721 cccgtaacgg tttaattttt atgatttcaa tacatcgccg catttcgcaa attttgtcgc
     3781 cccgaataaa atccgcgcag cagtttgaag ccaaatttat ttcccgtttg ccggcattgt
     3841 tttttcttct cgccgtcgcg gtcatttcca tcgatgcctc agcgcaggac aacccattcc
     3901 cggctgcagc cagcgttttg caaccgcaag gctacgtttc actgcagccc gtgcctcgcg
     3961 gccacgcatt tcaggttgcg gtcgtggcga aaatttcgcc cggctttcac atcaacgctc
     4021 accagccatc tgaagattat ctaattccga caaaggtgac tgctgaactt cctgcaggat
     4081 tgtttgtggt ggaaaccaat tatccccgcg gcgtgatgcg tgcgtttcgt ttttcgaaga
     4141 cgcctcttcg agtttacgaa ggatcgttta cggtgttaat gaagctacgt gcgaatggca
     4201 gtgcgccgct cggcccgcaa aaaattaatc ttactgttgg ataccaggcc tgcaatcagg
     4261 acgcttgcct gccccccaca aaaattccgt tgacggcgga tctggaaatc gccgcagcgg
     4321 atactcctgc aaagcctgcg cacacagata ttttctcctc gagttccgct gagaagtctc
     4381 ctcccaaacc ataatttgcg gtgtgtcttg tcggaatctt tatgcaaatg agatcagtgc
     4441 gcggccaaga tttccacggc gcgacgcaaa cgagtgacga cgcgatcccg gcccaaaacc
     4501 agcatggttt caaacagagg aggcgccaca gcctgcccga cgatggccac gcgtgtcgcg
     4561 ttaatcagca ggccagcttt tacaccttca gccgcagcca attcacgcaa tgcatggtcg
     4621 cacgcgtcgt gattccaatc gggaagcggc gccaacgcat ccgccagctt gtccagcatc
     4681 ggacccagac gctcgtcctt ccagaatttt tcgcgcgccg ccggatcgat ttcaaacgta
     4741 tcgctgaaaa atgcgcgcgc ccacgtcaca aaatcgggaa gcagccgtgt gcgcggccgc
     4801 agcaggtcca cggttttaga aaaccactcg cgcttttctc cagcccactc ggactgccac
     4861 agcttggcgc gctccagttc gccctgaatg tagggaagca gttcgtcgat cggcagcttc
     4921 tgcaaatact gtacgttgaa ccattccagt ttcgggcggt caaacacggc gttcgtgcgg
     4981 ctcacacctt ctagagaaaa tttctcaatg atttcagtgg tgcgcatgaa ttccgcatcg
     5041 ccaattgacc agccgaggag cgccagaaaa tttcgaaacg cttccggcaa gaacccttcg
     5101 tcgcggtagg agcccacact ggtagcaccg tggcgtttgg acaggcgtgt acgatccggc
     5161 cccagaatga gcggcacatg cgcaaaagta gggggcgtag cgcccagtgc gaggtacagc
     5221 agggcttgct tcggcgtgtt ggaaatatgg tctgagccgc gaatcacatg cgtgatcccc
     5281 atgtcgatgt catccactac aacgcttaat tgatacgtag gaccgccatg cgtctgacca
     5341 tccttgggcg agcgcagcac aacaaaatcc tcaatctcgt cattggacac ttcgcgcgag
     5401 ccgaaaaccg catcctcaaa tcgcgtaact ccgccaacgg gaacgcgaaa gcgtatcgcc
     5461 gcattttcag cgcttaatcc tggtttgcct tcccggcacg cacatcgagt aatgcgccgc
     5521 ggtccgggtt ctttttttac gtcttcagtt tcctcaaccg catccccacc tgcatatttc
     5581 tccggcgggc aatcgcagta ataggctgct cctgatgtgg caagtttaat cgccgcctca
     5641 cggtgcaagc ccaggcgctg cgattgaaaa tacggtcctt cgtcccaatc gaccccaagc
     5701 catcgcaacc catccagaat gccttccacg agctcggggc gattgcgctc aacatccgtg
     5761 tcctcaattc gaagtaccat cgtgccgctc ctgctgcgcg caaatagcca attaaatagc
     5821 gcggtccggg cccctcccac gtgcaaatag ccggttggcg acggcgcgaa tctcacccga
     5881 attttggcgg cggggtttcc cgatgcaacg gaagtcgtgg acatgcttct tggcagtctc
     5941 tcctggaatt gaaaagcaat tcatcttagc cgaacctgct tgggaaggcg agacgcagca
     6001 tcgggcaatt ttcatgtttg aaacatgttt gaaagaagat aaaggttcaa ttccggccca
     6061 taacttatgt gtccgatcac acacgtaact tgttccccga tggtacccca gtaccgttga
     6121 ccggccaaaa ggacgtccat atagtcgcaa acgtagggaa attgtcggga gaggtgtgaa
     6181 atctctgttg cggctacacc tattttctaa ggagggtgat tagctttgat gctttgtcag
     6241 agcataaatt cttcttcatg ttgaagtaac gcatttccga acagatggaa atgcgattta
     6301 caagcctgac ggcgaattgg gaccgatgac ttcggaaagc atattggcga ttaccctgct
     6361 ggctgcctca gcattgacgt cgggtttctt cgcctacatg cacagcttac ggcgccaggc
     6421 atacctgatg ctttggagcg ccggatggtg cctgctcgcg ctccattatc ttagcccggc
     6481 tctcgaacct tgggtttcag ttgctccgtg gcaggcatct gttaatgaat ggctactggc
     6541 cgcagctgcc ctgctttttc taagcactgc tcaggtctat acgaatcgcg tgccatgggt
     6601 tcacccccaa attgccgcgg gatgtgtgtt cgcggtttgg tcagccgcaa attatttgaa
     6661 tgtaaccaag atttcaccgc agctgggcgt cgccgcggtg ctcgcgttag tcgcctggat
//
LOCUS       DASZMB010000011         8990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3312568, whole genome shotgun sequence.
ACCESSION   DASZMB010000011 DASZMB010000000
VERSION     DASZMB010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8990
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3312568"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="VG272_00345"
     CDS             <1..1100
                     /locus_tag="VG272_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HWF12156.1"
                     /translation="QDMGYYPHGSFFFSDLTHYVRSGDFVQALIRDSKDLDGYAFALG
                     ALAHYAADNNGHPAGTNRAVPLLYPKLEKKYGDSVTYEEDRLAHVKTEFGFDVLEVAQ
                     GRYAPDNYHDFIGFGVSIPLLEQAFQETYGLDLKDVLSNENKVIGSYRHDVSKLLPRA
                     TRVAWSLKKDEIQKDQPGITKRRFLYNLSRASYRKNWGKDYQPLTLREKFLAFLIRIL
                     PKFGPLKVLQLRAPTPEAQRLFEASFNATMDNYRHLLSDVDTGKLDLPNKNFDTGSIL
                     PPGEYKLNDKAQAKLLDNLAKKNFTGASPQMRAELLEYLGHTDAPYAMKKNKKSWSKV
                     EAELDELRNAIPPDAATNNPLPSDEVSGSNK"
     gene            complement(1143..2243)
                     /locus_tag="VG272_00350"
     CDS             complement(1143..2243)
                     /locus_tag="VG272_00350"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HWF12157.1"
                     /translation="MNRPEQIRLMTVAALTIVISMGLAARAQQTQYPKQADLPNPYKL
                     IDNWPTLPANMNGGHWGELIRADIDPKGNIWVFHRCFNTEPAGYATCVGRTEPPILEF
                     DPSGKLLASFGSGMFAFPHGFTVDHEGNVWASDANNAETVLGISAKDANGVVRGQQVF
                     KMSSTGKILMTLGKEGVSGNSPDTFDMPTGIAIGTNGDIFVTDGHGKNDRVVKFSKDG
                     NFIKTWGHHGSSPGEFDQPHDISIGGSKGYVYVADRSNSRVQIFDQDGNFIAAWKQFG
                     RPSAVYVSKDDTLYVTDSQSNSTTNPGYLRGITVGSAKDGSLRAFIPDPDLAQADVNR
                     ISGASGIVADDKGTIYAADVGPHRLRKYVKIK"
     gene            2486..2797
                     /gene="rplU"
                     /locus_tag="VG272_00355"
     CDS             2486..2797
                     /gene="rplU"
                     /locus_tag="VG272_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HWF12158.1"
                     /translation="MYAVIRSGGKQYRVAPGDTVKVETLPGKVGGNITFGDVVAVATD
                     EKKLLTGSAAAKAKVTGKIVSQGRHPKLTVFKYRCIKQYKIVRGHRQNYTAVEVSGIE
                     L"
     gene            2818..3114
                     /gene="rpmA"
                     /locus_tag="VG272_00360"
     CDS             2818..3114
                     /gene="rpmA"
                     /locus_tag="VG272_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013203.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HWF12159.1"
                     /translation="MAHKKGGGSSVNGRNSPGQRLGVKRFGGQLVHAGTILIRQRGTK
                     HKPGVNVARGKDDTLFALIDGVVLFQDKGQDGRFISVMPPEQATEATPAVTPAN"
     gene            3315..4529
                     /gene="obgE"
                     /locus_tag="VG272_00365"
     CDS             3315..4529
                     /gene="obgE"
                     /locus_tag="VG272_00365"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HWF12160.1"
                     /translation="MVFVDEAKIYVKAGNGGNGCIAFRREKFVPRGGPSGGDGGHGGN
                     IFLEANPHDNTLLRYRYNREFKADRGRHGEGSNCSGHSGEDMILSVPVGTVVTDQDTG
                     ELIADLTKPGERILVAAGGRGGRGNQHFAKPWHQAPRESEEGQPGVEHNLHFELKLLA
                     DIGLVGFPNAGKSTLISRISAARPKIADYPFTTLQPGLGVVSADGDPGKPSEKGGGRT
                     FVVADLPGLIEGAHTGVGLGIRFLKHIERTRFIAHLIDTSDASDRDPVRDFEIIEGEL
                     TAFSEKLAEKPVMVVATKLDATTDRTRLDKLRDFCAARELEFHAVSSATGEGIPQLVR
                     AMANALDRLAPREPVVEAATESAQPPTMDGQPELPESESPALAPVAVVPEEPRRKVAR
                     APRKPRQKKEAH"
     gene            4526..5245
                     /gene="nadD"
                     /locus_tag="VG272_00370"
     CDS             4526..5245
                     /gene="nadD"
                     /locus_tag="VG272_00370"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HWF12161.1"
                     /translation="MNSAPPESHGVTHEQGRERRIALFGGTFDPIHIGHLAVARAAER
                     RFHLDEIHLIPSGRPPHKQRVRIASFADRYAMTSIACADHPRFIPSLVESGAGQSGRE
                     TFYSVDTVRTFKHKHHATRPHIYFLVGADSFLDIPAWKDYETLLGLCDFIVVSRPGFN
                     LDTLRLVIPPELFGRRGAAHAPLDPRAITLKRTVVYILDTVASHVSATEVRTRLDRNE
                     SIHGLVPQRVEEYITKKAIYR"
     gene            5242..5637
                     /gene="rsfS"
                     /locus_tag="VG272_00375"
     CDS             5242..5637
                     /gene="rsfS"
                     /locus_tag="VG272_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HWF12162.1"
                     /translation="MTPRKAPIPPKVRAAILAAEEKQAADIVLLDLEGLGAFTDYFLI
                     CTGFSSPQVAAICDEVEEQLERIGTHRIHREGKTGSDWMLLDFGGLVVHVFTERARHF
                     YDLERLWRAARRIEIRGTGEGSSLAEAEG"
     gene            5634..6566
                     /locus_tag="VG272_00380"
     CDS             5634..6566
                     /locus_tag="VG272_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWF12163.1"
                     /translation="MTEPRMVWIAEDPASKAVLELARKVAETSTTLLITGESGTGKDH
                     LARLIHELGPRRDAPFLKIDCASLPQELVESELFGHERGAFTGAVERKFGRLEMAGNG
                     TIVLDEVAALSPATQSKLLRVLEERRFERLGGTETITITARLIALTNADLSRLVSEGQ
                     FREDLFFRLSVLTVPVPALRARPADILPLAEALLARGARVHGRSTITFSDAVKKMLTQ
                     YSWPGNVRELRNAIERALVFSGGASLIPEDFPESVRTADSAFGGALRSLEDLEREAIR
                     ATLEATRYKIGKSAEILGISRKTLLDKRKKYGLN"
     gene            6591..7022
                     /locus_tag="VG272_00385"
     CDS             6591..7022
                     /locus_tag="VG272_00385"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HWF12164.1"
                     /translation="MKIHLVMLGKTRRPEIRALMDDYSARMERFAELQITELREDSAA
                     SIRKFAVDSAATVVLLDDAGKPQTSTQFAKWLGEMRDRGTRELVFLCGGAEGFPEALR
                     KRASQKLSLSPLTFSHELARIMLAEQLYRAFAILAGHPYPK"
     gene            7963..8679
                     /locus_tag="VG272_00390"
     CDS             7963..8679
                     /locus_tag="VG272_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12165.1"
                     /translation="MGNRGFTGSLNTSRSIQPLRGSNRFGVPFGGNRGRCWSCGNRFG
                     GWGNRWGWGWGFGSPWLGFWGWGWDPFIYDPWWGSGPWLGYGYYGYPNNYIYPDPGNY
                     APDYNSAPPEQQQDNSYDEDYQGNSNGNWVTPNEPSSSSLQHSPSMAVPILIYMKSGK
                     ILSVRDYWMVDGELHYILMSGVQNSVDLEQVDLPRTNTENAKSGVKFIFKSEPSAAPT
                     APDPAPTRELDAVPQPEAMT"
BASE COUNT         2063 a         2316 c         2533 g         2078 t
ORIGIN      
        1 ttcaggacat ggggtactac ccgcatggga gctttttctt cagcgatttg acgcactacg
       61 ttcgtagtgg cgattttgtt caagcactga ttcgagattc caaagacctg gatggttacg
      121 cctttgcgct tggggcgctg gcgcactacg cggccgacaa taatggacat cctgcaggaa
      181 ctaaccgcgc tgtcccgctt ctgtatccga aacttgaaaa aaaatacggt gattcggtta
      241 cctacgaaga agatcgcctg gcgcatgtga agactgaatt tggattcgat gtgctggaag
      301 tcgcgcaggg gcgttacgcg ccggataatt atcatgattt cattggtttt ggggtttcca
      361 ttcctttatt ggagcaggca tttcaggaaa catacggctt agacttaaag gatgttctct
      421 ccaatgaaaa caaggtcatt ggatcttacc ggcacgatgt cagcaagctt cttccgcgag
      481 ccacacgcgt tgcatggagc ctgaagaaag acgaaattca gaaagatcag ccggggatta
      541 caaaaagaag attcctctac aatctttcgc gcgcgagcta caggaaaaat tggggaaagg
      601 actatcaacc gctcacactt cgcgaaaagt ttctcgcgtt tctgattcgt attcttccca
      661 agtttggacc gctgaaagtg ctgcaattga gagcgcccac accggaagcg cagagattat
      721 ttgaagcaag ttttaacgcc acgatggata attaccgcca tttattgagc gatgtcgaca
      781 caggcaagct ggatttgccg aacaagaatt tcgacacagg ttcgattctg ccacctggcg
      841 aatacaaact gaacgataaa gcgcaggcga aactgctcga taatctggcg aagaaaaact
      901 ttacaggtgc gtctccgcag atgcgcgcgg aacttctgga gtatttaggc catactgatg
      961 cgccgtacgc gatgaaaaaa aataagaaaa gctggagtaa agtcgaagcg gagttggacg
     1021 aattgagaaa cgcgattcca cccgatgcgg cgacgaataa cccactccca tccgatgaag
     1081 tttcaggatc gaacaaataa aacattacga atagaccgtg aagcgcaacc caaaattttc
     1141 cgttatttga tttttacgta cttgcgtaac ctatgcggac ccacatccgc agcgtaaata
     1201 gtgcctttat catctgccac gattcccgag gcgcctgaaa tgcgattcac atctgcttgg
     1261 gccaagtctg gatcggggat aaacgcccgg agtgaaccgt cctttgcgct tcccactgta
     1321 atgcccctca gatagccggg attagtagtc gaattcgatt gcgaatcggt gacgtacagc
     1381 gtgtcatctt tagaaacgta tacagcgctg gggcggccga attgtttcca cgcggcgatg
     1441 aaatttccat cctggtcgaa aatctggacg cggctgttgc tgcgatcggc cacatatacg
     1501 tagcctttgg aaccgccaat ggaaatgtcg tgcggctggt cgaattctcc cgggctggaa
     1561 ccgtgatgtc cccaggtctt aataaaattt ccatcctttg aaaatttaac cacgcggtca
     1621 tttttgccat ggccgtcagt cacgaaaata tcgccattcg ttccaattgc aatgccggtt
     1681 ggcatgtcaa atgtgtcggg gctgtttcct gaaacgccct ccttgcccag tgtcatcaat
     1741 attttgccgg tagaactcat cttaaatacc tgctgaccgc gaaccacgcc attggcgtct
     1801 tttgcggaga ttcccaagac agtttcggca ttgttggcgt cgcttgccca gacattgccc
     1861 tcgtgatcga ccgtgaagcc gtggggaaat gcgaacatgc cgctgccgaa actcgctaaa
     1921 agctttcccg acgggtcaaa ttccaggata ggtggttctg tgcggccgac gcatgtggca
     1981 taacctgcag gctccgtgtt gaagcagcgg tgaaacaccc agatgttgcc tttgggatcg
     2041 atgtccgcgc ggatcagttc accccaatgg cccccattca tgtttgcggg aagcgttggc
     2101 cagttatcga tcaacttgta gggattgggc agatcggctt gcttggggta ttgagtctgc
     2161 tgggcacgtg cggccaatcc cattgatatg acaatagtaa gcgccgcgac ggtcatcaat
     2221 cttatttgtt ctggacgatt catctttaat gcctcggtgc ggacttcggt ccgcggagtt
     2281 tcgtttccca atcaaccatt cggtgtgtat ttgaattaaa tacttataca ccaacgcctc
     2341 tttcccgtga atcccgcaaa gagagctgcc catagatccc ttcgtctgcc aggttggttg
     2401 tgcctataga agcccacacc ttttcattga cgaagcgcgg aggcacccgt ataatcgggg
     2461 tttccgattt gtgtctggag gggcgatgta cgccgtaatt cgatctggtg gtaagcagta
     2521 ccgggtagct cccggagata cggtcaaggt cgaaaccttg cccggtaaag tgggcggaaa
     2581 cattacgttc ggcgacgtcg tcgccgtggc gacagatgaa aagaagcttt tgaccggttc
     2641 ggcggccgcg aaggccaaag tgactggcaa gatcgtttcg cagggacgcc atccgaaact
     2701 tacagtattc aagtaccgct gcattaaaca atacaagatt gtccgcggcc accggcagaa
     2761 ctacacagcc gttgaggtta gcggaatcga gctctaagag ctccaaagga atctgagatg
     2821 gcacataaga aaggtggcgg ctcctccgta aatggccgca attcacctgg acaaagactg
     2881 ggcgtaaagc gatttggcgg ccagcttgtc catgcgggaa caattttgat tcgtcagcgt
     2941 ggcacgaaac acaagcccgg cgtgaacgta gcgcgaggca aggacgatac gctttttgcg
     3001 ttgattgatg gcgtcgtgct gtttcaggac aagggccagg acgggcgctt catcagcgtt
     3061 atgccgcccg agcaggcaac ggaagcaacg cctgccgtca cgccagcaaa ctaactggat
     3121 ctcgaattcg agaaggcggc tccgcggatg cggggccgct tttttatttt cattcgacga
     3181 aatgcctctt tcgtggcata ggcttcagcc tgtggaattt agttctttct aggccaaaac
     3241 cccacaggct gaagcctatg ccacagaagt gcaaatgatt cctgagtgtc gtattctgaa
     3301 aacggaagtg aaaaatggtt tttgttgacg aagcgaaaat ttatgtgaaa gccggcaacg
     3361 gcggaaacgg ctgcattgcg tttcggcgcg aaaaatttgt gccgcgcggc gggccttccg
     3421 gcggcgacgg cggccatggt ggtaacattt tcctcgaagc aaatccgcac gacaatacac
     3481 ttttgcgcta tcgctacaat cgcgagttca aggcagatcg cggccggcac ggcgaaggtt
     3541 ccaattgctc cggccattcg ggcgaggaca tgatcctctc tgtccccgtt ggcacagttg
     3601 tgaccgatca ggatacgggc gaattgattg ccgatttgac aaagccgggc gagcgcatat
     3661 tggttgctgc cggcgggcgt ggcggacgcg gcaaccagca tttcgcgaag ccctggcatc
     3721 aggcgccacg cgaatcggaa gagggccagc cgggcgtcga gcacaatctt cattttgaat
     3781 tgaagctgct cgcggacatc ggattggtcg gctttccgaa tgccggaaaa tcgacattga
     3841 tttcgagaat ttccgcagcg cgtccgaaaa ttgcggacta cccattcaca accttgcagc
     3901 ccggtctagg agttgtttcc gccgatggcg atccgggtaa gccttccgaa aagggtggcg
     3961 gccgcacgtt tgtagtggcg gacttgccgg gattgatcga gggtgcgcat acgggtgttg
     4021 gactcggtat tcgtttcctg aagcatatcg agcggacgcg ctttatcgcg catctgattg
     4081 ataccagcga tgcttccgat cgcgatcccg tgcgcgattt cgaaattatt gagggtgagc
     4141 tgaccgcctt cagcgaaaag ttggcagaga agcccgtgat ggttgtggcc acgaagcttg
     4201 acgcgaccac ggatcgaacg cggctggata aattgcgcga tttctgtgcc gcgcgagaac
     4261 ttgagtttca cgcggtttcc tcagcaaccg gtgagggcat accgcaactc gtgagagcga
     4321 tggcgaatgc gctcgatcgt ttggctccgc gtgaaccggt ggtggaagca gcgactgaaa
     4381 gtgctcagcc gccaacgatg gacggccaac cggaactgcc ggagagcgaa tctcctgcac
     4441 tcgcacctgt tgcggttgtc cccgaagagc cccggcgaaa ggtcgcgcgc gcaccacgca
     4501 aaccccgcca gaaaaaagag gcccattgaa cagcgcgccg ccggaatcgc acggcgttac
     4561 acacgaacag ggccgggaac ggcgcatcgc gcttttcggc ggcacgtttg acccgattca
     4621 tatcggccac ctggccgtgg cgcgagcggc agagcgacga tttcatctgg atgaaattca
     4681 tctcattccc agcgggcgtc caccgcacaa acagcgcgtg cgaattgctt cctttgcgga
     4741 tcgttatgcc atgacgtcca ttgcgtgtgc cgaccatccg agatttattc catcgctcgt
     4801 ggaatccggc gctggacaat ccggacgcga gactttttat tcagtggata ccgtcaggac
     4861 ctttaaacac aagcaccatg caacgcgccc acatatttat tttttggtgg gagctgattc
     4921 ctttctggat attccggcct ggaaagatta cgaaacactg ctaggccttt gtgattttat
     4981 cgtggtgagc cgaccgggct ttaatttaga cactctgcga ttagtaatcc cgcctgaact
     5041 ttttgggcgc cgtggcgccg cgcatgcgcc actcgatccc cgcgcaatta ccttgaaacg
     5101 cacggtggtt tacattcttg acacggttgc gagtcacgtt tccgcgaccg aggtccggac
     5161 gcgattggat cgaaacgaat cgattcatgg acttgtgcca cagcgcgtag aggaatacat
     5221 tacaaagaag gccatttacc ggtgacccca agaaaggctc ctattccgcc caaagttcgc
     5281 gcggccattc tagccgcgga agaaaagcag gcggcagaca ttgtcctgct tgatctcgaa
     5341 gggcttggcg cgttcaccga ttattttctg atctgcaccg gattcagttc gccgcaggtt
     5401 gccgcgattt gcgatgaagt ggaagaacaa ctcgagcgga taggcaccca ccgaatacac
     5461 cgcgaaggaa aaaccggtag cgactggatg cttctagatt ttggcggcct cgttgtgcac
     5521 gtattcaccg aacgtgcgcg ccatttctac gacttggagc gtttgtggcg cgctgcgcgg
     5581 cgcatcgaaa tccgaggaac cggcgaaggg tcttccctgg cggaggcgga aggatgaccg
     5641 agcctcgcat ggtctggatc gcagaggatc ccgcgtccaa ggccgtactg gaactggcac
     5701 gaaaagttgc ggaaacttct acaacgcttc tcattaccgg agagagcggc acgggaaaag
     5761 atcacctcgc gcggttgatt catgaacttg gtccccggcg cgacgctcca tttcttaaaa
     5821 tcgattgcgc cagccttccc caagaacttg tcgagtcgga attatttggg cacgaacgcg
     5881 gcgctttcac gggcgctgtg gagcgcaagt ttggccggct ggaaatggcg ggcaatggaa
     5941 caatcgttct ggatgaagtc gccgcgcttt ctccggcgac gcagtcaaag ctattgcgtg
     6001 tgctcgaaga acggcgattc gagcggctgg gtggcactga aacgattacg atcaccgcgc
     6061 gcctgattgc gttgaccaac gcagatctct ctcgcctggt ttccgaggga caatttcgcg
     6121 aggatttatt ttttcggctg agcgtactta cggttccggt gcccgcgctg cgtgcgcggc
     6181 cggctgatat attaccgctg gccgaagcat tactcgcgcg cggagcacgt gtgcatggcc
     6241 gttcgacaat tacattttca gatgcagtga aaaaaatgct tacacaatat tcctggccgg
     6301 gaaatgtgcg cgaacttaga aatgcgattg agcgcgctct ggtattctcc ggcggggcgt
     6361 ctctcattcc ggaagatttt cccgaatccg tgcgaaccgc ggattccgcg ttcggcggcg
     6421 cgctgaggtc gcttgaagat ctggagcgcg aagccatccg cgcaacgctc gaagcgacgc
     6481 gctacaaaat tggcaagagc gcggaaatat tgggaatcag ccgcaaaacc ctcctggata
     6541 aacgcaagaa atacggcttg aactgaaaat cctgtgcggt aaatatcttg atgaaaattc
     6601 atttggtgat gctcgggaaa acgcgacggc cggagatacg cgcgttgatg gacgattact
     6661 cggctcgcat ggaacgtttc gccgagttac aaattaccga gttacgcgaa gattccgcgg
     6721 cgtccattcg caagttcgcg gttgattccg cggctacggt tgttttgctt gatgatgcgg
     6781 gaaagccgca aacgtccacg cagtttgcga agtggctagg agaaatgcgc gatcgcggca
     6841 cacgcgaact cgtattttta tgcggtgggg cggaaggttt tccggaggca ttgcgcaagc
     6901 gcgcttcgca aaaactttct ctttctcctc taacgttctc gcacgaactc gcgcggatca
     6961 tgctcgccga gcaactctac cgagcctttg ccattctcgc cggtcatccc tatcccaaat
     7021 aattgaacgg aagaatcata gcggaacaag aattggagga aacctttgga atttgctgcg
     7081 atggggttct tggatcgttc gccacttctt ccaggacaca cggggggcga actctaactg
     7141 ctacaaaggt ttaaatgagc gcaaccaatg catagggtgt agtatctaat caggtgtaaa
     7201 gcaaaaacga gttcccgcat aactcgtccg taggtctagc gaagtttccg cggaggcctt
     7261 gtcatgaagc cattttcggt aatccttaaa tctgccttgg caattttgtt tatttctatt
     7321 gctggcccag cgtttgccca gcatggcggg ggtgggcacg ggggcggtgg tggaggcggt
     7381 tttcacggtg ggggcggtgg gggaagtttt catggcggtg gtggaatgcg ctcgagcggc
     7441 gggagcgcgc cccgtgggcg gtccttttcg cctcccccct ccggacatgg ggccccaaga
     7501 gctgccgctc cttctggtcc tcaattcgaa aacggttttg cgggtcggca agggaataat
     7561 ttttcgaggc caggcggcaa ttttgcaaat ggtaatgagc gggttggaaa ttcgcactct
     7621 gcacctggcg tggacggtgg ctggcattcc tttggtggca accagggcgg aggacgccag
     7681 gccgctggta cacaatctca gccggggtca ccgaataatg ccggcaactt ccacgttttc
     7741 aacggaaatc gcgggacagc accaaacagt gcggctcgca gcttttcagg acagggtggc
     7801 gaagtctggg aaaattccgc agctgcccga aacatggtcc caaaatccca atcgctttcc
     7861 acactccata attctttcgt aggctcgcgc tcagccacgt cagggctgcg tccaaacgcc
     7921 gcgctttcgg cttcctcacg tttcgcaggc ggttcggcat ttgtgggtaa tcgcggattt
     7981 acaggcagcc tgaatacctc caggtcaata caacctctgc gcggttcgaa tcgattcggc
     8041 gtccccttcg gaggaaatcg cggacgatgc tggagctgcg ggaatcgatt tggcgggtgg
     8101 ggaaatcgct ggggttgggg ctggggattt ggctcacctt ggttaggctt ctggggatgg
     8161 ggctgggatc catttattta cgatccgtgg tggggaagcg gcccttggct gggctatggc
     8221 tactacggct atcccaataa ttacatttac ccagatcctg ggaattacgc gccggattac
     8281 aattcagctc cgcccgagca gcagcaggac aattcctacg atgaagacta tcaggggaat
     8341 tcgaatggta attgggtgac gcccaatgag ccgagttctt cctctttgca acactctccg
     8401 agcatggcgg taccgatcct catttacatg aagagcggca aaatattatc ggtgcgcgat
     8461 tactggatgg tggatggcga actgcattat attttgatga gcggcgtgca gaattccgtc
     8521 gatctggagc aggttgattt gccgcgcacc aatacggaaa atgcgaagag cggcgtgaag
     8581 tttattttca aatcggagcc gagtgctgcg ccgacggcgc ccgatcctgc tcctacgcgg
     8641 gaattggatg cggttccgca gccggaggcg atgacgtaga ttttagtagc gcgggcttca
     8701 gaactgcaaa attggaactt cagcggctaa agaggctgcg gaaaaactca acattttgtc
     8761 attccgagcg aagcgagaaa tccctccttc tttattttct tatacttaaa tcgaagagag
     8821 attcctcgct tcgctcggaa tgacagaaca aactactttt tccgcaaact gtaaagccgc
     8881 attcattttg cagaacttat gtcgtggcta aagccacgac ccacaaagat tcttcagttc
     8941 tcacacagac tcttcagcct gcggagtttg gtcgatgttt gggcttaccc
//
LOCUS       DASZMB010000012        20338 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3313047, whole genome shotgun sequence.
ACCESSION   DASZMB010000012 DASZMB010000000
VERSION     DASZMB010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 20338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20338
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3313047"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..1493)
                     /locus_tag="VG272_00395"
     CDS             complement(210..1493)
                     /locus_tag="VG272_00395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF12166.1"
                     /translation="MPDRLRPAIRHLRLNWIDLIWLIFLGALALLPPVDEIHKQLILL
                     AIFAVQLSEGWLVSRLPQRGPAYAVIIKILLATLLLDHTGDFGINSSYYPIYFLPVVS
                     AAIYFGTWAALAWTTLASLAYLSLLIPALQEVDVTPEGKGILAIRVLFFYIAAVLVNR
                     FAVENRKHVQRLEDLSGKLEETNRQLLRAEADARRAERLAALGQLSAGLAHEIRNPLG
                     VIKGSADMLSRKVAQSEPLVGELAGYISSEVNRLNALVVRFLDFARPSKLELRPEQIS
                     EIVDRAVTAAIASIPDSGVRIEREYAPNLPPVTVDAQLCEQVFINLITNAIQAMQGQD
                     AALEKTLRLSILPEVSSGESGIGVIVEDNGLGVPPELREQIFNPFFTSKKAGVGLGLS
                     IVAKIVDDHRGTIRLEDNTPRGARFHVFFPQAATN"
     gene            complement(1646..1954)
                     /locus_tag="VG272_00400"
     CDS             complement(1646..1954)
                     /locus_tag="VG272_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12167.1"
                     /translation="MQTESNTPIVPAVAADNETRHDFDFPQREAAFFYGLFLRGHSAE
                     ELRRDIEVPPAVLLKWHRESERDPSLRDIFTRMLDYRRHVLAIFDVLVDTESRPQRVQ
                     "
     gene            complement(2224..2502)
                     /locus_tag="VG272_00405"
     CDS             complement(2224..2502)
                     /locus_tag="VG272_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12168.1"
                     /translation="MWNKNDWDQFFAIASKPWQRRRPPRPVYPSGAKRVLPAVGFSLS
                     ELDDAGINMEAAERLGLPVDAARIGAYGPNVSALKEYVRSARNPSKLG"
     gene            2938..4182
                     /gene="thiI"
                     /locus_tag="VG272_00410"
     CDS             2938..4182
                     /gene="thiI"
                     /locus_tag="VG272_00410"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HWF12169.1"
                     /translation="MSKLVIVVHYHELWLKGGNRRFFLSKLHIALKRALEGLPVERIL
                     RPGDRLLIEFGEGASAEEAVARIERVFGVAFYAVARVVPRVGENDLAALGRAAWEEIR
                     GEKFATFAVRSKRSDKSFPARTIDIEVSVGQYLVDQLRAAGRQARVKLNDPDLTCRVE
                     IPPGPSLLVYARKIPGAGGLPPNTAGRMTCLLSGGFDSAVAAHKMMKRGAHLNFVHFW
                     GGGADPGESSVYVARDLVKRLVPWQFSAKLYLVPFEPIQREIVARAPEEFRILLYRRM
                     MLRIAERVARSNRALGLITGDSLAQVASQTLHNMAAVGAAVRMPLYRPLVGDDKMEIM
                     DAARKIGTHDISAEPFHDCCPIFLPRNPALHASVEELDRAESTLDIEALIRQGIDTLA
                     IERYRYGNGQVEKVESFKSATA"
     gene            complement(4944..5195)
                     /locus_tag="VG272_00415"
     CDS             complement(4944..5195)
                     /locus_tag="VG272_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HWF12170.1"
                     /translation="MATLYVENVPDDLYEALRNRAKGSRRSMAAEVLAMLEESIPTAR
                     ELRSRHEWVRKLARLRKQHAGAVRSFPSAEEMLRQDRAR"
     gene            complement(5301..5501)
                     /locus_tag="VG272_00420"
     CDS             complement(5301..5501)
                     /locus_tag="VG272_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12171.1"
                     /translation="MADVVPEALAEAAVEAAVPVEAEVALVVVAGAVVAEEAAVAAVV
                     AEIATEVIEAVVVVAGNWPCLT"
     gene            6063..7682
                     /locus_tag="VG272_00425"
     CDS             6063..7682
                     /locus_tag="VG272_00425"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HWF12172.1"
                     /translation="MRGLMMDFPLTVPPILERAGKTFGNVEIVTRKPDRSVERTTWGN
                     LYKRSHRLANALTKLGLRRGDRVATLLWNQAEHMEAYFGVPIAGGVLHTLNVRLHPEE
                     IAFIATHAQDRFLIVDDVLLPIYEKFRENVKFERVIVVPYGGCEIPDGCNNYENLLSE
                     SSEVFAYPRLEENEAAAMCYTSGTTGRPKGVVYSHRAIVLHSMSCCGVDSLGVSMRDT
                     MLPVTSLFHANGWGIPFTSAMAGAKIVLGGPFADGEGLLDLMEQERVTVTTGVPTVWF
                     GVLQALEKFPGRWKIDWPVRMHVGGAPTSEALMRGFDRHGIAVVCSWGMTETTPVATS
                     CHLRPFMDCWSEAKKYTQRTKPGTAAAFLEARIMNLDGTPAPHDGSTVGELEVRGPWV
                     AAEYYHMPEEARKWSADGWLRTGDMGHIDEYGYVKLVDRSKDLIKSGGEWISSVDLEN
                     ALMGHPAVKEAAVVGVPHPKWQERPLAVVVLCEGARAEPEELREFLATKFAKWQLPDA
                     IVFADEIPRTSVGKFRKTALREQFVDWKWED"
     gene            complement(7759..9852)
                     /gene="pheT"
                     /locus_tag="VG272_00430"
     CDS             complement(7759..9852)
                     /gene="pheT"
                     /locus_tag="VG272_00430"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HWF12173.1"
                     /translation="MKVLYNWLKEYVELTAAPEELRTRLSLSGTAVEALDQTAAGPML
                     DAELTSNRADCLGHYGIAREAAVLYGLPLKPVNPRPRESSERAVAATRVQIDAPELCG
                     RYTARVLRGVKVGPSPDWLRQRLEALGQTSINNVVDATNYIMLELGHPLHAFDLDLLA
                     EKRIVVRRARAGEKMCTLDGIERTLTNEMCVIADAAHPVAIAGVMGGADSEIRSTSKN
                     ILLESAWFDPISIRRTSKTLGLRTEASMRFERGADLEMAATASRRCAELIQQLGGGEV
                     LAGVIDVYPGRSEEPKIVLTRQEFLRVMGADVSDTEIESILAGLGFAPVRSDTARSIT
                     GSPDAAWKCVRPSWRGDVTREVDLIEEVARIYGVDKFPARLPPAKLPAARLEYAEADD
                     RLREILIGLGYQEIIAIPIVDEPTDALFRPESAVPARIANPLAEDASVMRSTGAVTMA
                     RTLEWNLNHGQRNVRLFEFGKTYGWNGTQPVETRVLTIGASGLAREKGVAETERSYAF
                     ADLKGDLDQIGHLAGGIAWKPGGPEWLQGAHAGTISLRANGATASRPIGHAGQISRRI
                     SERFKLRQDAYIAELELEPLCAGYKTARAALRYKPLSRFPAVERDFSLIVADGTAFAG
                     VEEAIRGLGIAEVSSIEAVDLFRAKNMPEGKFALLVRVKFESLQATLTEAQLTDFSSR
                     ILAALEQNLGATLRA"
     gene            complement(10161..11270)
                     /gene="pheS"
                     /locus_tag="VG272_00435"
     CDS             complement(10161..11270)
                     /gene="pheS"
                     /locus_tag="VG272_00435"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HWF12174.1"
                     /translation="MDANPISAGKILPPLDAATPEQVVVCFADLRAQFDRDAAGATNE
                     SGWKALRDTWLGRKSGLLTQVTDTWLKPAKPELKRAVGQSLNELKGHVEAALESRRAT
                     IEAAAESATNAREREDLSLPGVIRALGSRHPLRQTFEEIERIFLSLGYSVIDGPEMET
                     PYYNFEALNIPEHHPARDNMDTFYMATPPNAAPLLLRTHTSPMQIRTMEKQPPPVRII
                     VPGKVYRRDNPDATHGFMFHQIEGLAVDTGITFCDFKGTVEYFVREFLGPNVKTRLRP
                     SYFPFTEPSVEVDATCVFCGGKGCNVCKHSGWIELFGAGMVDPAVYGFVNYDAKKLSG
                     FAFGLGVERLTQLKYNINDLQLYFQNDVRFLRQFA"
     gene            complement(11418..11795)
                     /gene="rplT"
                     /locus_tag="VG272_00440"
     CDS             complement(11418..11795)
                     /gene="rplT"
                     /locus_tag="VG272_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR01032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HWF12175.1"
                     /translation="MARVKRGTKRRARRKKFLKRTKGFFLTKSKLYQSAQEAVNRAER
                     FSYRDRRVRKRQFRTLWIQRIGAAARNAGISYSQLMHGLKAAGVEIDRKVLSDIAVKD
                     AAGFLQLVDQAKASVNANPKAKA"
     gene            complement(12399..12617)
                     /gene="rpmI"
                     /locus_tag="VG272_00445"
     CDS             complement(12399..12617)
                     /gene="rpmI"
                     /locus_tag="VG272_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HWF12176.1"
                     /translation="MAKKKAKKLKLKTHRGAAKRFKITSTGKVMRMHSGKRHLLGTKK
                     ANRMRRLKKLKEVHPADAGNVHKMLPYG"
     gene            complement(12745..13347)
                     /gene="infC"
                     /locus_tag="VG272_00450"
     CDS             complement(12745..13347)
                     /gene="infC"
                     /locus_tag="VG272_00450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HWF12177.1"
                     /translation="MFGGGFIAERSFRQADRIRINDRIRAREIRVVDEDGGQLGVMPP
                     FEAIKLAKERGLDLVEISPTADPPVCKITDYGRYLYQTNKKAHEQRKHQKGSQLKEVK
                     FRPATAEHDFQVRKNQIIRFLGEGHKVKAMIFHRGREMAHQEVGRAKMHRLLQEITDH
                     ALVEIGPRMEANVLMALLAPKRGGGAPPAKPAGAPAGGQS"
     gene            complement(13575..15923)
                     /locus_tag="VG272_00455"
     CDS             complement(13575..15923)
                     /locus_tag="VG272_00455"
                     /inference="COORDINATES: protein motif:HMM:NF027952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWF12178.1"
                     /translation="MSTADSLPAGARTILGTVPPQAAGHFVVFTAQPAAGRMNLLESD
                     LATGQVKRVTSDNDQALVLAGGPSGNPLLSSNFFLSDTGEIAFETVNFGRAIGLSAFN
                     FGSSPTNNSWENSAACGNIYLWSASGIITKVAAAGDPVPNSSAKFSCVALNSGPPSPL
                     NHSGELTFFSSSPLASLVGCTFCNTPPGNSVLGIDGIFLYHPGGSITEIAAADDTLPG
                     QTQATTFVPYLATPLNSADQVAFGAQVGTTSQAFYLKNGSSAQSVISLGDSVPGGTDT
                     FGFPHFISGLADNGNLTFTAATSSVIDGLFLAPSGGPIQTLALQGGAAPASGGGVYSL
                     PDLVLGSISVSGSPPVTIYSFKNFAEMNSESDVAFGSAITGGSADSGYFRMLQSGPSA
                     GTLQAVVAQGQAVPGGGTLSTIPTTSAAGGTFALGPDGSLAFVNPLTTSSGLEQGMFV
                     ARPDGTLLKVASTGDVLPGGEVLSGISMSPKLSAGNAGTFAFLGGILGGSARSGIFVT
                     AIPPGVAATTVTLSPLLATAVAQQPTAFSATVVGPVPGSPSGTVTFFASGISLGTGTL
                     NSSGQASISAPTLAAGQDSIVAQYSGDANFAAANSSPLAIVVAGFAPPQSPLVVTRGQ
                     SLVIPITLYAPATPAMSFTLSCSGLPANASCSFDNNPVTPGPTGTTVHLTLTTMAGAK
                     LPLGLPHKEVPPLLGYELATLLASILVAKLFWRRAPRWRLVPCACFATFALAFLMGGC
                     GASGAGSYNSVTTGTPAGPTTVTVTGISGNTTISTVVNVIVQ"
     gene            complement(16832..17578)
                     /locus_tag="VG272_00460"
     CDS             complement(16832..17578)
                     /locus_tag="VG272_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12179.1"
                     /translation="MSQHFLSFAQTFREDILFRGLTKTCATFVAILVLFSATAKAQGE
                     VPLIPVATDQSALSLSNQFGIPASSAINQAGDFAFVGNGDTALFLRFAGTSAATRLLQ
                     IDDELPNFPGSHIQFILPGLGINSSRILFFGARFTGADGASYSALMTYAEASYRTIVT
                     SYSFIPNSNTSTYGVNLIPGSIDDSGDVNFAAVPTGSGSLVYYIAPAGFTTAFELPAR
                     QIHLRHPARGARPLEVLWEVFCRGYPSPTE"
     gene            18046..18399
                     /locus_tag="VG272_00465"
     CDS             18046..18399
                     /locus_tag="VG272_00465"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HWF12180.1"
                     /translation="MDKKRVDYYKKKLQARREELIKSIARTEEEGRTADDDPTVDLAD
                     KAANSYTKEFLFGQTNVDRNLLALIDGALARIKAGTYGVCSECDEELQQRRLEAVPWA
                     RHCVSCAEKHEQGLL"
     gene            19519..20142
                     /locus_tag="VG272_00470"
     CDS             19519..20142
                     /locus_tag="VG272_00470"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWF12181.1"
                     /translation="MKPPGQIGRPPRPKHSPEVRGESRPLGGIPPVVRKPAIMQEPVL
                     VLNATFEPINVTAVRRAMVLMLKGVAQAEEINHTKVHSASRSLEVPSVIRLLAYRHIP
                     QQTRALSRKNILLRDRNTCQFCRSILPASELTLDHVMPRSRGGRSSWENLVACCYQCN
                     NRKGDRTPEEAGLALARRPRPFTLHTSRQLMRLIGNKDEKWRKYLFY"
BASE COUNT         4705 a         5522 c         5612 g         4499 t
ORIGIN      
        1 gcgaagcgag gaatctctct tcgatttaag tataagaaaa taaagcagga gggattcctc
       61 gcttcgctcg gaatgacaaa atgttgagtt tttccgcggc ctgtaaagcc gaactgaatt
      121 tgcaggactt atgtcgtggc taaagccaca acccacaacg atcctgcagt tctcactcag
      181 actccgaagc ttgcgccccg gaaaactcct cagttcgtag cagcttgcgg gaagaataca
      241 tggaagcgtg cgccacgtgg agtattgtcc tcgagacgaa ttgttccgcg atggtcgtct
      301 acaatttttg caacgatgga aagtcccaga cccactccgg ctttttttga agtaaaaaac
      361 ggattgaaga tttgctcgcg caattctggc ggcacgccca ggccgttgtc ttcgacgatc
      421 acaccgatac cggattctcc acttgaaact tcaggcaaga tcgacaaacg caaagttttc
      481 tcgagcgcgg catcctgtcc ctgcatagct tgaatggcat tcgttatcag attgataaaa
      541 acttgttcgc ataactgcgc gtcaacggtt acaggcggca aattcggagc gtattcccgc
      601 tcgattctca cacctgaatc cgggatcgac gcaatggcag cagtaaccgc gcgatcgaca
      661 atctcggaaa tttgttccgg gcgcaattcg agctttgagg gccgcgcgaa atccagaaac
      721 cgtacaacaa gcgcattcag gcggttcact tccgaggaaa tataacctgc cagttcgccg
      781 acgagcggtt cggactgcgc aactttccgg ctcagcatgt cggcagaccc tttgataacg
      841 ccgagcggat tacgaatttc atgcgcgagt ccagcggaga gttgtccaag agcggccaac
      901 cgttcagcgc gacgcgcatc cgcttcggcg cgcagtaatt gacgattcgt ttcttcgagt
      961 tttcccgaaa gatcttccag cctttgcacg tgtttccgat tttcaactgc aaatcgattg
     1021 accaaaacgg ccgcgatata aaagaacaga acacggatcg caaggatgcc tttgccctca
     1081 ggcgtaacat ccacctcctg gagcgcagga attagcaaag aaaggtaagc cagcgaagcg
     1141 agcgtggtcc aggccagcgc cgcccacgtg ccgaagtaga tggcggcgct cacaacgggc
     1201 aaaaagtaaa tcgggtagta gctgctgttg ataccaaaat cgccggtgtg atcgagtaac
     1261 aacgtggcga gcaaaatttt aatgatgacg gcgtacgctg ggccgcgttg cggcaagcgg
     1321 ctgaccaacc agccttcgct caactgtacg gcgaagatcg ccagcaggat cagttgctta
     1381 tgaatttcat cgaccggagg gagaagcgcc agtgcgccta aaaaaattag ccagatcagg
     1441 tcaatccagt tcagacggag atggcggatg gctggtcgta ggcggtccgg cattctgaga
     1501 ggctcccgca aagtctaaca tggcgggctt gttccgcggt tggggtgttg gttcgattct
     1561 ggtttggaag gtctaaaccc acacaggctg aagccggtgt gctacaggtg aacgcgcaat
     1621 agtccattgt gggtggaaga ttttcttact gaacccgttg agggcgcgat tcggtatcaa
     1681 ccaggacgtc gaaaatcgcg agcacgtggc ggcgatagtc gagcatgcgc gtgaaaatgt
     1741 cgcgcaagga aggatcacgc tccgattcgc gatgccactt gagcaacacc gccgggggaa
     1801 cttcgatgtc gcggcgaagc tcttccgcgg aatgtcctct cagaaacagg ccatagaaga
     1861 atgcggcttc gcgctgcggg aaatcaaagt cgtgccgtgt ttcattatcc gctgcaacgg
     1921 caggcacaat cggcgtgttg gattcagtct gcatttatac tcaaagacag aaaggagatt
     1981 ttaccggaag ttttaacggc gtcaagtagc tgggccgaga aataacttgg ttttatgagg
     2041 tttgaagatg tatctcacct caagccgggt caaaatccta gacaccaaaa tttaggcatt
     2101 aaccgatatg caattcaggc tccagagtgt ggattttggc ttcgacaaca agcaaaatcc
     2161 acaggctgaa gcctgtgcta ctaaaatcaa tgtgtttcgc tctttacgtt tgcgcttcgc
     2221 cgtttatccc aatttactgg gattgcgcgc agaacggaca tattccttga gtgctgaaac
     2281 gttcggaccg tacgcgccaa ttcgggccgc gtccacgggc aatcccaaac gctcggctgc
     2341 ctccatgtta atccctgcat catcaagctc ggagaggctg aaaccgactg ctggaagcac
     2401 gcgcttcgca ccactcggat aaaccgggcg tggaggtcgg cgccgttgcc agggcttgct
     2461 tgcgatagca aagaactgat cccaatcgtt cttgttccac atcgctcgct cccgcgtctc
     2521 atggaccagg acgctctagc tctcaagcaa tcagatgcac gaataacgct tgtggtcgct
     2581 tttcgtcgcc acggcccatt tggactgggg cggcgtcctt tacttgggta tacgacacaa
     2641 caccgcaggc gtccagaata gactgagaat agttacagga gcgttacgaa agagaaatta
     2701 tcgcgttgga atgagctgct gttccgaaag cgaacgcgtc ggttttctgg ttggcaccgc
     2761 gtccgaaggt tgaatttcgt ttcacgtgaa acatctcgcc aactcaatgc ggacgttgct
     2821 tttgcgacct ttctcaaatg gcttgcccat ggttagttga tcgcgcagtg ttagccaatc
     2881 cttacgcacg gctgttatac tggcggatat tcgagcatct ttagggaatt ggtatacatg
     2941 tcaaaactgg ttatcgtcgt ccactatcat gagctatggc ttaagggagg aaatcgccgc
     3001 tttttcttga gcaagctgca tattgcgctg aagcgcgcgc ttgagggtct cccggtggag
     3061 cgcattctga ggcccggcga tcgattgctc atcgaatttg gcgagggagc ttcggcagaa
     3121 gaagccgtgg cgcgcatcga gcgcgtgttt ggcgtcgcat tttatgccgt ggctcgagtg
     3181 gttccgcgcg taggtgaaaa tgatctggct gcattaggac gcgccgcgtg ggaagaaatt
     3241 cgcggagaaa agtttgcgac gtttgcagtg cgctccaagc gcagtgacaa atctttcccg
     3301 gcgcgcacga tcgatatcga agtttctgtt gggcaatatc tggtggatca attgcgcgcg
     3361 gccggacgcc aggcgcgtgt gaaactcaat gatccggatt taacgtgccg cgtggaaatt
     3421 ccgccagggc cgtcgctgct cgtttacgct cgaaaaattc ccggcgcggg cggacttccg
     3481 cccaataccg ccgggcgcat gacgtgcctg ctttcaggtg gattcgattc cgctgtcgcc
     3541 gcgcacaaaa tgatgaagcg cggcgctcat ttgaatttcg tgcacttctg gggcggagga
     3601 gcagatcccg gagagtcatc tgtgtacgtg gcgcgtgacc ttgtgaagcg ccttgtgcca
     3661 tggcaatttt ccgcaaagct ttatctcgtg cccttcgagc cgatacagag ggaaattgtg
     3721 gcgcgtgcgc cggaggaatt tcgaattttg ctctatcgcc gcatgatgct gcgcattgcc
     3781 gagcgtgttg cgcgatccaa tcgtgctttg ggcctcatca ccggagacag tctggcgcag
     3841 gtcgcttcac aaacgctgca taatatggcg gccgttggcg cagctgtgcg catgcctctc
     3901 taccgcccgc tggtgggcga tgacaaaatg gaaatcatgg atgccgcgcg aaaaatcggc
     3961 acgcatgata tttccgcgga accgtttcac gattgctgcc ccattttttt gccgcgtaat
     4021 cccgccctgc acgcttccgt cgaagaactc gaccgcgcgg aaagcactct cgacatcgaa
     4081 gcgctgatcc gccaaggcat cgacacgctg gcgatagagc gctaccgcta cggcaacggc
     4141 caagtggaaa aagtggaatc gtttaaatcc gccacggcgt aaaatgccaa gaaaatccaa
     4201 acggaaatat ctaaactttt cttccgagat gactgacact ctgcaatcgt atgtctacat
     4261 ttatactgac ccccgaaacg gagaagtttt ctacattggc aaaggtcggg ggaatagagc
     4321 attttcacat ctcaacgagt ttggggaaac agaaaagatt gctaggataa acgccatacg
     4381 caagtccggg gcgaagccaa aaatagatat actgcgttac ggaatgtcga acgaagaatc
     4441 gtcactcgtc gaggcggcgg taatcgactt tgtaggcctt tcgcgcttaa ctaatgcggt
     4501 ccgggggaat caccgagcaa gcttggggcg agtgtcttcc aaagatctaa taactatgct
     4561 cacggcgaag ccggcgaaaa tcaaacataa gaccatcttg ataactatca atcgccttta
     4621 tcgaagtgat atgacagtcg aagaattgta tgaggctact cgcggaatct ggaggatagg
     4681 cccgaagcgc gaaaaggcag aacttgttgt agctgtctat cagggaatcg taagagaagt
     4741 ctatagagtc gcgcgatggc tgcctgccgg caccctagtg tacaaaacgc gcgacactcg
     4801 aggtttcaag ggttccggca ggtgggagtt cgacggcaaa atcgcccatg acattcgcga
     4861 tcaatactta gacttctctg tccgccattt attaggaaac aaaagtcaga acccaattag
     4921 atattcaaga ggatggtagg tagtcatcgc gccctgtctt ggcgcaacat ttcttccgca
     4981 gaggggaatg agcgaactgc cccggcgtgc tgtttgcgca gccgagcgag tttgcgcacc
     5041 cactcatgcc gtgatctcag ctcccgtgcc gtaggtatgc tttcctcaag catagccagc
     5101 acctcagcag ccattgaacg gcggctgcct ttggcgcggt ttcggagcgc ttcgtagaga
     5161 tcgtcaggaa cgttttcgac gtatagtgtt gccataggaa tctccagggg aaagcataga
     5221 taactccggg attctaggca aggcacaaaa tagaaacggg gagccttttg agctccccgt
     5281 tggtcagatg ctttgtgaat tcaggtcagg cagggccaat taccagcgac caccaccacc
     5341 gcctcgatca cctcggtcgc gatctctgcc accaccgccg ccactgccgc ctcctcggcc
     5401 accaccgcgc ccgccaccac caccagggcc acctcggcct ccaccgggac cgcggcctcc
     5461 accgccgcct ccgccaaagc ctccgggacg acgtccgcca cctccgccgc cgccttcgcg
     5521 cgggggcctg gcttcgttta ccacgatatt acgtcccatg aaatcatgcc cattcaatcc
     5581 ttgaacggcg cgatccgctt cctcgtcgtt attgatttca acaaatccga acccgcgtgg
     5641 ctgacccgag aaacggtcgc gtaccaatgt gacgttggct gcggtaactc ccgtttggga
     5701 aaatagagtg gtcagttgtt cttctgtagc ctgaaatggc agattgccaa cgtaaagttt
     5761 cttcaacgcg aggctccttt tggccgcgga agtacgaatc cacgaaaccg aggactgaag
     5821 aaggcgggat cgagattagg gacatgggct gagacccaga ataatccgat atgaggcgca
     5881 cttcagctca gagcgaatct tcctccaaat ttgaaggtcc gtcaacataa acatttatgt
     5941 ataacttcct agtaaatagg aaggcgatct atcttatttg atggggaccc tgcgggaaat
     6001 tccacgaatt tgatgatagg atgcggcatt catgcaagct gtgttaaggg gaaatagcaa
     6061 tcatgcgcgg gttaatgatg gattttccgc tgaccgttcc gcccatactc gagagggcgg
     6121 gaaaaacgtt tggcaatgtc gagatcgtca ctagaaaacc tgaccgaagt gtggagcgga
     6181 ccacgtgggg aaatttgtat aagcgctcgc accggctggc aaatgccttg acgaagctcg
     6241 ggttgcgccg cggagaccgc gtggctacgc tcctgtggaa tcaagcggag cacatggaag
     6301 cttatttcgg tgtgccgatc gcgggcggcg tcctccacac attgaacgtg cggctccatc
     6361 ccgaggaaat cgcgttcatc gccacccatg cccaagatcg tttcctgatc gtcgacgatg
     6421 tgctgctacc gatttacgaa aaattccgcg agaatgtgaa attcgagcgc gtcattgtcg
     6481 ttccctatgg aggctgcgaa attccggacg gctgcaacaa ttatgagaat ttgctatccg
     6541 aatccagcga agtgttcgcc tacccgcggc tcgaagaaaa tgaagcagcg gccatgtgct
     6601 acacgtccgg cacaacaggg cggcccaagg gcgttgtgta ttcccaccgc gccatcgtgc
     6661 ttcactccat gagctgttgc ggtgtggatt cgctcggcgt ttccatgcgc gacacgatgc
     6721 tgcctgtcac gtcgttattt catgcgaatg gttggggcat tcccttcact tcggccatgg
     6781 ccggagccaa gattgttctc gggggaccct tcgcggatgg agagggcctc cttgacctga
     6841 tggagcagga acgcgtgact gtgaccaccg gcgtgcccac cgtgtggttc ggcgtgctgc
     6901 aagcacttga gaaatttccc gggcgctgga aaatcgattg gccggtgcga atgcatgtgg
     6961 gaggtgcacc gacatcggaa gccttgatgc gcggtttcga tcggcatgga attgccgtcg
     7021 tttgttcgtg gggcatgacg gaaacaactc cggtagctac aagctgccat ttgcggcctt
     7081 ttatggattg ctggagcgag gccaagaaat atacgcaacg cacaaaacct ggaacggctg
     7141 cggcatttct cgaagcgcgc atcatgaatc tcgatggcac gccggcgccg catgacggca
     7201 gtacggttgg cgagctggaa gtgcgtgggc cctgggtggc ggcggagtat taccacatgc
     7261 cggaagaggc gcgtaagtgg tcggcagacg gctggctgcg cacaggcgac atgggccaca
     7321 tcgacgaata cggttacgtg aagctggtgg atcgcagcaa agatttaatc aaatcgggcg
     7381 gagaatggat cagctcggtg gaccttgaaa acgcgctcat ggggcatccc gcggtgaagg
     7441 aagcagccgt tgttggagta ccgcatccga aatggcagga acgcccactc gctgtggtgg
     7501 tgttgtgcga aggtgcgaga gcggagcccg aagaattgcg agaatttctt gccacgaaat
     7561 ttgccaagtg gcaattgcca gacgcaattg tgtttgccga tgaaattccg cggacttcgg
     7621 tggggaaatt ccgcaagaca gcattgcgcg aacaatttgt ggattggaag tgggaagact
     7681 aggttgaatt ggatttacgt gtcgtggggc ttcaggccgc gccacataag cccgcaagta
     7741 ttggaatttt ttaagaatct acgcgcggag agtggcgcca agattttgct cgagcgcggc
     7801 gagtatgcgc gaagaaaaat cggtgagctg agcttccgtt agagtggcct gcaaactttc
     7861 aaacttcacg cgaacgagca gcgcaaattt tccctcgggc atattctttg cacggaaaag
     7921 atccacagct tcgatcgagg aaacttccgc aatacccaaa ccgcggattg cttcttcgac
     7981 gccggcgaat gcggttccat ccgcgacaat gagtgaaaaa tcgcgttcca ctgccggaaa
     8041 acgggaaagc ggtttataac gcagcgcggc gcgcgcggtc ttgtatccag cgcagagcgg
     8101 ctcgagttcc agttcggcga tgtatgcgtc ctgccgcaat ttgaatcgct cggaaatgcg
     8161 ccgtgaaatt tgcccggcgt gcccgatggg ccgcgaagct gtcgcgccgt tggctcgcaa
     8221 cgaaattgtg cccgcgtgcg cgccttgcag ccattctgga ccgcccggct tccacgcaat
     8281 tcctcccgcg agatgcccaa tctgatcgag gtcgcctttc aaatccgcga acgcgtaaga
     8341 gcgttccgtc tctgcgacgc ccttttcgcg cgcaaggccg gatgcaccga tcgttaatac
     8401 gcgcgtctcg acaggctgcg ttccgttcca accgtaggtc tttccaaatt caaacaggcg
     8461 cacgttgcgc tgcccgtgat ttaaattcca ttccaaggtt cgcgccatgg tgaccgcgcc
     8521 ggtcgagcgc atcacggaag catcttcggc cagcggattc gcgatgcgcg cgggcactgc
     8581 ggattccggg cggaacagcg cgtcagtcgg ttcatccacg atcggaatcg caataatttc
     8641 ctgatagcca aggccaatta aaatttcgcg caggcggtca tcggcttccg catattccag
     8701 gcgcgccgct ggaagttttg cgggaggcag ccgcgcggga aatttatcca cgccgtagat
     8761 tcgcgcgact tcttcgatca ggtccacttc gcgcgttaca tcgccgcgcc aggatgggcg
     8821 tacgcatttc catgccgcgt cgggagatcc ggtaatactt cgcgccgtgt ccgaacgcac
     8881 gggcgcaaaa ccgagtcccg cgagaatcga ttcgatctcc gtatcggata catccgcgcc
     8941 catcacgcgc aggaattcct gccgcgtaag cacaattttt ggctcttcgc tgcgtcccgg
     9001 ataaacgtcg attacacccg cgagcacttc gccgccgccc aactgctgga tgagttcggc
     9061 gcagcggcgc gaagccgtcg ccgccatttc caggtccgcg ccgcgctcga aacgcatcga
     9121 ggcctccgtt cgcaagccaa gtgttttgga agtgcggcga atggaaatgg gatcgaacca
     9181 tgcggattcg agcagaatgt ttttgctggt ggaacgaatt tcgctatccg cgccgcccat
     9241 caccccggcg atggccacgg gatgcgcggc gtcggcgatc acgcacattt cgttggtgag
     9301 cgtgcgctca atgccatcga gcgtgcacat tttttctcca gcgcgagcgc ggcgcaccac
     9361 gatgcgcttc tcggccagca aatctaggtc gaacgcatgc agcgggtgcc cgagttcgag
     9421 catgatgtaa ttcgtggcat ccaccacgtt gttgatggaa gtttgtccca gcgcttcgag
     9481 gcgctgccgg agccagtccg gcgacggccc taccttcacc ccgcgcagga ctcgcgcggt
     9541 gtagcgccca cacaattcgg gagcgtcgat ctgcacgcgc gtcgccgcca ctgctcgttc
     9601 ggaggattcg cgcgggcgcg gattgacggg cttgagcggc aagccgtaca acacggccgc
     9661 ttcgcgcgca atgccgtagt gccccaggca atccgcgcga ttcgacgtca gctcggcatc
     9721 aagcatcggc cccgctgcgg tctggtccaa cgcttccacg gccgttccgg aaagcgagag
     9781 ccgcgtgcgc aattcttccg gcgccgctgt cagctcaaca tattctttta gccagttgta
     9841 gagaactttc attgttcagt tttcgtccca gatacccatt gagccgcgac ggaaacaaat
     9901 cttttttagg tcgcggctct gcgggtggtg gctttgtttg tcacgtgttc gattgtcagg
     9961 tgtttgtggg tcacggcttc agccgtgaca taaattactc gctcagttcg gctttagccg
    10021 ctgacgcaca taattgtgcg ccgaactttt agggctaaag cccacatttc aaagccgata
    10081 atgtcggagc taaagctccg acccacaaaa cataactcac aaatttcaat ggtacgaatt
    10141 cactgcgtac aaaaatactt ttacgcgaac tggcgcagga aacgcacatc attctgaaaa
    10201 tagagctgca aatcattgat gttgtatttc agctgcgtca ggcgctcaac gcccaggcca
    10261 aacgcgaaac ccgaaagttt cttcgcatcg taattcacga agccgtagac ggcgggatcg
    10321 accatgcccg cgccgaacaa ttcaatccag cctgaatgct tgcacacgtt gcagcccttg
    10381 ccgccgcaaa atacgcatgt ggcatccact tcgacggatg gttcggtgaa gggaaaatag
    10441 cttgggcgca ggcgcgtctt cacgttcggc cccagaaatt cgcgcacgaa atattcgact
    10501 gtacccttga aatcgcaaaa cgtgatgccc gtatcgaccg cgagtccttc gatctggtgg
    10561 aacataaaac cgtgcgtcgc atccggatta tcgcggcgat acactttgcc gggtacgata
    10621 atgcgcaccg gcggcggctg tttttccatg gtgcgaatct gcatcggcga agtgtgcgtg
    10681 cgcagcagga gcggcgcggc atttggaggc gtggccatat aaaacgtatc catattgtcg
    10741 cgcgcggggt gatgctcagg aatattgagc gcctcaaaat tgtagtaggg agtttccatt
    10801 tccggcccgt caatgacgga atacccgagc gaaagaaaaa ttcgttcgat ttcctcgaac
    10861 gtctgccgca gcgggtggcg cgagccgagc gcgcgaatca cgccgggcag cgagaggtct
    10921 tcgcgttcac gagcattggt ggcgctttcc gcggccgctt cgatggttgc gcgccgcgat
    10981 tcgagcgcag cttcgacgtg ccccttcagt tcgttgagcg attggccaac tgcgcgtttc
    11041 agttcaggct ttgcaggttt gagccaggtg tcggtgactt gcgtgagtaa accggatttg
    11101 cggccaagcc acgtatcgcg cagcgctttc caaccgctct cattcgttgc gcccgcagca
    11161 tctcgatcga actgcgcgcg caagtcggca aagcacacca ccacctgctc gggcgtggcg
    11221 gcgtcgagcg gtgggagaat ttttccagcc gaaattggat tcgcgtccat tgggcaagaa
    11281 tttaattccg caatgctttt gtagcccgcc tctcttgtcc tgagtgaaaa cgaaggatca
    11341 gaggcggggt ttagacgatg cgacatctca aatgcgcccc atctaagtcc ccgcctctga
    11401 agaggcgggc tacagcacta cgcttttgct tttgggttgg cgttgaccga tgctttcgcc
    11461 tgatccacca gttgcaggaa tcccgcggca tccttcacgg cgatatccga tagcaccttg
    11521 cggtcgatct cgacgcccgc tgccttcagc ccgtgcatca actggctgta ggaaattccg
    11581 gcgttgcgag ccgccgcgcc gatacgctga atccacagcg tgcggaactg gcgtttgcgg
    11641 acgcggcgat cgcggtagga aaaacgctcc gcgcgattga ccgcttcctg ggcggactga
    11701 tagagcttcg actttgttag gaagaaacct tttgtgcgct ttagaaactt tttgcggcgc
    11761 gcgcgtcgct ttgtaccgcg ttttactcga gccattttat tttctcctgt gcggttcacc
    11821 gcgagggttc accgcgagcg gagacgcagt gcaatccgct acatcatggc aagtccccgc
    11881 gcacgggaac cggcacacaa tcggaaagag agcgtctcca cggcgtgttt tagaagctca
    11941 gcgcaaattc cgcgtgaagc gaatttttcg cgagcaatac ccggtcctga attctgattg
    12001 gaccgaaccg ctggccggca ggcccaccgt ggcgggttgc gctctgcctg gcacagcaaa
    12061 aaaatcaaaa attgctttga aactttaacc cgaaatcttg ccttaaaatc tagccttgtg
    12121 ggtcggacct tcaggtccga catttcaggc tcgccgcacg aggcctttag gccctgagcc
    12181 ttcaggggct aaagcccatt tttcaaccca acttattgtc agggctgaag ccctgaccca
    12241 caaaccctaa ccatcaaacc ctgagtcaca aactatggcg aacaaaccct gactcgcaaa
    12301 cgttgaccca caagcgcggt cagcaaatct cactcgactt ccgtaaatcc gcaatccaaa
    12361 aaatcattta ttgcttcgtc tctattgtcg tttcacttct agccgtacgg taacatcttg
    12421 tgaacattgc ctgcgtcggc tggatgaact tctttcaatt ttttcaggcg gcgcatgcgg
    12481 ttggcttttt ttgtcccgag caggtgccgc ttgccggagt gcatgcgcat tacttttcca
    12541 gtggaagtaa tcttaaaccg cttggcggct ccgcgatgcg tcttgagctt cagtttcttt
    12601 gctttctttt ttgccacaga cgtttcctct ctaaattaca aaacgaaaca ggcaggcttc
    12661 agcctgtctg tttttgagtt gatcttaaaa gaataattta aaaaaaccgc tgacaggaaa
    12721 aaccctgtca aactgccgcg gtgctcagct ctgcccaccg gcaggagccc ccgcaggttt
    12781 cgccggcggc gcaccgcctc cgcgcttcgg cgcaagcagc gccatcaaaa cgttcgcttc
    12841 catgcgcggc ccaatttcca ccagcgcgtg atccgtaatt tcctgcaaca gcctgtgcat
    12901 tttggcccgg cccacttctt gatgagccat ttcgcggccg cgatgaaaaa tcatcgcctt
    12961 tactttgtgc ccttcgccga ggaaccggat gatttgattc ttgcgaacct gaaaatcatg
    13021 ttccgcggtt gccgggcgaa acttgacttc cttgagctgc gagccttttt gatgcttgcg
    13081 ctgctcgtgt gcttttttat tggtttggta gagatatctt ccgtagtcgg taatcttgca
    13141 aaccggcggg tcggcggtcg gtgaaatctc caccagatcc agtccccgtt ctttcgcaag
    13201 cttaatcgct tcaaacggcg gcatcacacc cagctggccg ccatcctcat ccaccacgcg
    13261 aatttcacgc gcccgaattc gatcgttgat gcgaattcga tctgcctgac gaaaactccg
    13321 ttccgcgata aacccacctc caaaaatcgc gccactagtt caaaaaatgc tggccctgcg
    13381 gcgacttctt ccgtccaatc cctccctcgc acagtattct gctcaggatc aggctgggcc
    13441 acaaagtata gcacggccgg aagccctgca ataccaagag ttacgtagaa gcttaatttc
    13501 gaacgtaatc cttgttgatt atcggcatgt aattacagtc aggcttagtt agaatgacgc
    13561 aaattgataa gtctctattg gacgattaca ttcacaacgg tagaaattgt cgtgttccct
    13621 gaaattccag tgaccgtaac tgttgtcggg cccgccggtg tgcctgtcgt aactgaatta
    13681 tacgagccag cgccagacgc gccgcaaccg cccatgagga atgccaaagc gaatgttgcg
    13741 aagcaagcgc aaggcaccag ccgccatcgg ggcgcgcgcc gccaaaataa tttggccacc
    13801 aagatcgaag caagtagtgt agccaattca taacccagca gaggtggaac ttctttgtgc
    13861 ggcagaccga gtggcagttt cgcgcccgcc atcgtagtga gcgtcaggtg aacggttgtg
    13921 ccggttggac ccggcgttac cggattgttg tcaaatgaac agctcgcgtt cgcaggaagg
    13981 cctgaacagc tgagcgtaaa actcatcgcg ggagtcgctg gcgcgtaaag ggtaatggga
    14041 ataacgagac tttgtccgcg agtaacgacc agaggcgatt gaggtggcgc aaatcccgcg
    14101 acaacaatgg caagcgggct tgaatttgcc gctgcaaaat tggcgtcgcc gctgtactgc
    14161 gcgacgatcg agtcctgtcc cgcggccaaa gttggggcgc ttatcgatgc ttggccgctg
    14221 gaattcagcg ttccggtgcc aagcgaaatt ccactggcaa aaaacgtaac tgtgccagaa
    14281 gggctcccag gcacgggacc aacaaccgtt gcagaaaatg cagtaggctg ctgagctacc
    14341 gcagtggcca acaatgggct gagagtgaca gtggtcgcag caactcctgg cggtatggct
    14401 gtaacgaata ttccgcttcg agcgctgccg cccaaaattc ccccgagaaa tgcaaaagtc
    14461 ccggcattgc cagcagacag cttcggactc atggaaattc ctgacaaaac ttctccgccg
    14521 gggagaacat cgccagtgga agccactttc aagagagttc cgtccgggcg cgcgacaaac
    14581 atgccctgtt ctaggcccga gctggtagtc aatgggttga cgaaggcgag cgagccatcc
    14641 ggcccgagcg cgaaagtccc gcccgctgcg ctagtcgtcg gaatcgtgct taaagttcca
    14701 ccgcctggca cagcctgacc ttgtgcgacg actgcttgca atgtgccggc tgagggtccg
    14761 ctctgcaaca tgcggaagta tccggagtcg gcgctgccgc ctgtgatggc cgagccgaaa
    14821 gccacatcag attcgctgtt catttctgca aaattcttga aggaatagat ggtcaccggc
    14881 ggggagccgc taactgagat cgatcccaag accaaatccg gcagcgaata cactccgccg
    14941 cccgaagcgg gagccgcacc cccttgaagc gcgagcgttt gaatagggcc tccggagggt
    15001 gcaagaaata atccatcgat tactgaactt gtggcagcgg tgaaagtaag gtttccgttg
    15061 tccgccaggc cggaaatgaa atgagggaag ccgaaagtat cagtgccgcc cggaacagaa
    15121 tcgcccagcg agattacact ttgcgcagaa gatccatttt ttaggtaaaa ggcttgtgac
    15181 gtagttccca cttgggcgcc gaacgcgact tggtctgcag aatttagagg ggtcgccagg
    15241 taagggacaa acgttgtggc ttgggtttgt cccggcaacg tgtcatcggc agccgcgatt
    15301 tcggtaatgg atccgcccgg atggtagagg aatatgccat ctatgcccaa gaccgaattg
    15361 cccgggggag tgttgcaaaa cgtgcagcca accaaacttg caagaggaga gctactaaaa
    15421 aatgtcagct caccagaatg attcagcggt gagggtggtc cggaattcag cgcgacgcat
    15481 gagaactttg cgctggaatt tggcactgga tccccagccg cggccacttt cgttatgata
    15541 cctgaggcgg accaaagata gatgtttccg catgccgctg aattctccca gctattattt
    15601 gtggggctcg atccgaaatt aaatgccgat aatccaatcg ctctgccgaa attgactgtt
    15661 tcgaatgcga tctctcctgt gtcgcttaag aaaaaattgg aagacagcag tggatttcca
    15721 gaaggacccc cggcgaggac gagtgcctga tcgttgtcgc tagttacgcg cttaacttgt
    15781 cccgtggcca aatcagattc caggagattc attcgtccag ccgctggctg ggcggtgaac
    15841 acgacaaagt gcccggctgc ctgtggagga accgttccga gtatggtgcg cgcaccggca
    15901 gggagtgaat ccgcagtgct catcagggtt tgaacatttc ctggcgttcc caaatattcg
    15961 gcaaaatctg cagtggcatt tggcaaatac gatacgaaaa acacagaatc attgtttagt
    16021 atttgcgatt gaacattgaa tgaaaaaggg gcgatagacg caaagaggag gcttccacca
    16081 gcttctacaa tcggagcact accattattg gcctgagaaa taaattctgc agtggcggca
    16141 ctaggcttct gcctgtaaat tgcgctcccg ccattcgata aaacagtgta gaaacttacg
    16201 gttgcatcat ttgcgatgga tgcagcagaa aatggggtca aggaagacac ggaaatgatc
    16261 cccccggaac ccgaagaacc accacaccct accagggaga agaatcctcc attcgttcct
    16321 ggtgcctgct cacaatcata agcaactaga tctgactgcc cggatgcggc ctgatatcgg
    16381 agaagtgcaa aggtcgtaac agtgcttccc gagatggggg attgaaagac cagatcccct
    16441 gaatcgtcga atccaagcgc aactggcgag atcatcgtgc cgccgccaac gctagcaggc
    16501 gctgtgtttc ccgctgtaat gacagcggtc ggaaaggggg gtgcaactcc cggcgacccg
    16561 ggcatcgcta cgcaaattgc gacactcgtc gaaattggtg gattcgtgaa agcgaccgcc
    16621 ccactgttgt taagaaaagc tgtgtttcct tgcaatgaac ttaagtagag tacattggcg
    16681 gccggttgcg ggctacatgc tcccgtagga gccatgggta ccagagcgaa gctgccatct
    16741 tttccgccaa tgaaaaggcc tccccaaagt cctaaaagca tctggccttg cgaattcaat
    16801 ttcggaacgt aagccgtgaa aatttccgca attactccgt cggtgacggg tacccccgac
    16861 aaaaaacttc ccaaagaact tccagaggcc gtgcaccacg tgcaggatgc cggaggtgta
    16921 tctgccgagc cggcaattcg aaagctgttg taaatccggc cggagcgatg taatagacga
    16981 gcgagccaga accggtgggc acagccgcga agttcacatc gccgctgtca tcgatgctgc
    17041 caggaataag attaactcca taagtactgg tgttcgaatt ggggatgaag ctataggagg
    17101 tcacaatggt ccggtaagac gcctccgcat aagtcattaa cgcggaatag gacgcgccat
    17161 cggcacccgt gaatcttgct ccaaaaaaaa ggatgcgcga agaattgatt cccagccccg
    17221 gcaatatgaa ttgaatatgg ctccccggaa agttaggtag ttcatcgtca atttgcagga
    17281 gtcgtgtggc agcggatgtt cccgcaaagc gcaaaaataa ggcggtgtcg ccatttccca
    17341 cgaacgcaaa gtcgcctgcc tggttgatag cggagctggc tggaatccca aactgattcg
    17401 agaggctaag agcgctttga tccgtggcaa ccgggataag aggtacttct ccttgcgcct
    17461 tcgcagtggc ggaaaaaagt accagaatgg ccacgaatgt cgcgcaagtt ttcgtcaggc
    17521 cgcggaacaa tatatcttcg cgaaaggtct gagcgaacga cagaaagtgt tgggacatgg
    17581 ccatactagt accttcctat tgataatgac tggaaaaagc ctgctgcgaa aaaagtacag
    17641 aaggttttta acagtaattc atcggatcgt caatcattgg gaaagtaaac gtacagtgaa
    17701 tacctggatc tttgaaaggt tatttattcc tccaagcaag gcttctttcc gcagccgaag
    17761 aattgtgagt tccaacaagc cgacaggcga aaaaactcgc ccctgtattt ttgtaaatta
    17821 caggccaaag attgtttata ggattatctt gtccaagacc tactcaggtt aacttgtatt
    17881 tattttttaa agtgaaggca gtcaagggtg actgtcccgc aaatttctcg aaccagctcg
    17941 gcatgaatct ccagattaaa acccgcttcc ttgacagttg caagacgcgg gcctacaatc
    18001 acccgtttgt aatggtggtg attgggtgaa tggtgggccc ctacgatgga taagaagcgt
    18061 gtggattatt acaagaaaaa attgcaggca cggcgcgaag agctgatcaa gtccattgcc
    18121 cgtaccgaag aagaaggccg cactgcggac gacgatccaa cagtggatct ggcggacaag
    18181 gctgcgaatt cctataccaa ggaatttttg tttggacaga cgaacgtgga caggaattta
    18241 ttggctctga tcgacggagc gctggcccgg atcaaggctg gaacgtatgg cgtctgttcc
    18301 gagtgcgacg aagaacttca gcaaaggcgg ttggaagctg tgccctgggc acgtcactgc
    18361 gtgagctgcg cggaaaagca cgagcaagga ttgctgtaga ttcaaggcgg acattccgcg
    18421 aattgaattc ttccttaaaa gatcagcacg caacttgtac ggtttgcgta gcgtgggctt
    18481 cagggcgtgt gtgagaactg cgcgattggg acctcagccg ctaaagccgg actcattttg
    18541 caggatttat gtcgtggcta aagccacaac ccacccacaa cccacaacga gtttgaaggt
    18601 tctcacacag actctaaggc ccgcgtcact taacgcgtgt cacgaatcgt gccacttttc
    18661 acttccctcc tgacgacaag cgcggtaggc ttggagcact gtggcaaagg ctgccgatgc
    18721 ggttcgctcg cagattcgcg gaatatgcga ggcgtttgcc tcgctcgttt ttcctgcgcc
    18781 ttgccggatc tgcgactgca tcctggacac tggcagccca attccttttt gccacgaatg
    18841 tatcgaagct attcaacagc cgctgcggga accactttgc tcgaagtgcg gcaggccgat
    18901 cgtttcgaca gctgtgacgg aaggaatttc cctccccctt tgccacctct gtagaagcgg
    18961 ggtgtatgcc tttgaaatgg cgcgcagttt tggcgcctac acgcctcgaa tgtcccgcgc
    19021 cattctgctg ctcaaatacg ggaacttggc tccgctggga ggatggtttg cccgagtcct
    19081 tgcggatttg attcgaaggc agcctgcgga ctttgctgct gacgcggtgg tccctgttcc
    19141 gctggaccga acaaggtttc gggaaagggg atataaccag gcagagctta ttgctaagcc
    19201 cctagcccgc cttctggata tcccttttcg atcctatttg ttagttcgca cgcgcccgag
    19261 acccaatcac ctgcgtctca cgagacgcga gcgatgggaa acggtccgtg gcgcttatgc
    19321 tccatgtgaa cgagcgccgg ttgacaagct acgggtttta ctggtagatg atgtatttac
    19381 caccggggca actttagatg cctgctcccg ggccctgaaa ggggctggag ccgcccgagt
    19441 gatgggtctg accgttgcca gagcgttacc tctctacccg gcttcggcag ccggcttaca
    19501 agaccaggac tttggggaat gaaaccaccg ggccaaatcg gtaggcctcc ccggccaaag
    19561 cactcgccgg aagtccgagg cgagtcgcgc ccgcttgggg ggattccgcc agtagttcgc
    19621 aagcctgcca tcatgcaaga gcccgtcctc gttctaaacg caaccttcga gccaatcaac
    19681 gtcacagccg ttcgccgcgc catggtcctc atgctcaagg gagttgccca ggccgaagag
    19741 atcaaccaca cgaaggtgca ctcagcgtcc cgatccctcg aagtcccgtc cgtgatccgg
    19801 ctgctggcct atcgccacat tccccagcag acgcgcgctc tttcgcgcaa gaacatcctt
    19861 ttgcgggatc gcaatacatg ccagttctgc aggtcgattc ttcctgcctc cgaacttaca
    19921 ctggatcatg tgatgccccg gtctcgcggc ggcaggtcgt cgtgggagaa cctggtggcc
    19981 tgctgctacc agtgcaataa ccgaaaaggg gaccgcaccc ccgaggaagc cgggttggca
    20041 ttagctcgac ggccgcgacc gtttaccctg cacacgtccc ggcagctaat gcgcctgatc
    20101 ggcaataaag atgaaaaatg gcgcaagtat ttattttact aaggtttgat tgcaattccg
    20161 ggtatccccg ctggtttccg gcaataagaa aactccaaaa aatctaaaat ttttctaata
    20221 cgagcaacca tttcgttttt ggcgcgtcta actagacaaa ccacttcaag gacacacccc
    20281 gggaagcagc gatggctagt ccaaagctgc ttctcctttt ttacgcccat ttctcatc
//
LOCUS       DASZMB010000013         7102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4958115, whole genome shotgun sequence.
ACCESSION   DASZMB010000013 DASZMB010000000
VERSION     DASZMB010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4958115"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VG272_00475"
     CDS             complement(<1..645)
                     /locus_tag="VG272_00475"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWF12182.1"
                     /translation="MNWLALSPGWVAAIWAGLAALALWLYLHHRRPQHRKVSTLRFWA
                     SIQPISQPRRRKLREPWAFLAQILFLLLLILALANPRWGPAFEGRSVALVLDASIWSQ
                     AHPAGDSAWIDRERAEALRVLNSLPSGDRVLLLRAEAGAPPILPFTADRAALRRAIQN
                     VQPSSIAADIPSALETGRAALGGARRGLLVYVGPGLIHADEERGFDEFRAQLESP"
     gene            complement(642..1538)
                     /locus_tag="VG272_00480"
     CDS             complement(642..1538)
                     /locus_tag="VG272_00480"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWF12183.1"
                     /translation="MATTSPLLLDREFLKTLEQVTLLCRTNLSGAVGPEHRSRTRGPG
                     MEFADYRRYSFGDDPRSLDWSAYLRFGKLFLKIYETEQHIPVRILMDCSDSMGFGDAG
                     ESKFLYAQRLAATFAYLALLHLDTTAVIPFADRLKKPLVVSGGRDRLWPVLQFLSEQK
                     CGGTTDVLTSVKEFLGNFPSRGIVILVSDFFDEEGCESAVEMLRSAGHDFVLLQVHSS
                     EEQHPTASGELILEDAETGLHRTVECSPQSAALYERRFQEFSARLRRLAIRNGGRYAR
                     AVTNIAYQEFVLRGLRSGQVLA"
     gene            complement(1645..2763)
                     /locus_tag="VG272_00485"
     CDS             complement(1645..2763)
                     /locus_tag="VG272_00485"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWF12184.1"
                     /translation="MSQNSPEMPCESGKFGASGRREDEVEVEHKDEFMTSSVGTEGQP
                     GSQPLGEQAEFLSEKMAEVRREISKAIVGQAEVVEGVLIALLAGGHVLLEGAPGLGKT
                     MLVRTLAQAVALKYSRIQFTPDLMPADVIGTSVVQENGDGRKSLGFQPGPIFTNLLLA
                     DEINRATPKTQSALLEAMQEKQVTVAHTSHLLDEPFFVMATQNPLEMEGTYPLPEAQL
                     DRFLFKLRVPYPGREDLHDILDRTTQGTSATVNPVVNGAQILEMRRVVRAVPVAPHVQ
                     EYVVKLTLATHPENRENVLASKYIRYGSSPRGAQTLVLGGKVRALLDGRYNVSMEDIR
                     ALMLPALRHRIILNFEAQADGRAPDDLLEEIAGKISAK"
     gene            complement(2760..4211)
                     /locus_tag="VG272_00490"
     CDS             complement(2760..4211)
                     /locus_tag="VG272_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12185.1"
                     /translation="MNQGQSRENQILLARIFDHQARSPELNGLIDTLKPIRQRILANT
                     ILQGWVKWFSWILAVLAVVAAVSSKLFAPIVEVGILALTVAIGILAVIWRDRPSHYEV
                     ARRLDSAASLKDRLSTAIYLGAVQDTDGVVGSQRKDALSRLGKVDRRELFPFEWPGNA
                     KRAAALVLIVAGLFAYRVHHQPPLISLLQTTAHSQLVQSILSPIARAIEKDIQRTFAL
                     ASAKPDTLGEETKRAEEAATDDLWKADEKSPSTDQQKNTADAGDQPQDQMQPPGDQEG
                     TPSADGRPQDSNSQAQESKNGNESNDGKSQEQSEQTGPQGRQSVSQSLMQALKNMMSK
                     SPNQQSNNNRANQQPPNSQGSPQSGNSNQPGATESDRRGDSKGSSDAKQKASQTASEG
                     AGSQQGTKDLRTDLESHPVNAVPDRVALESSGFKEQTRMKVATETGTAQLALRDQAPQ
                     QDAVTNGSEQENIPARYRFYVQRYFEHADNGKH"
     gene            complement(4894..5271)
                     /locus_tag="VG272_00495"
     CDS             complement(4894..5271)
                     /locus_tag="VG272_00495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWF12186.1"
                     /translation="MIETVATDRGPKAIGPYSQAVRANGFVFLSGQIPLDPKTQQIVE
                     GDIAVQTERVMENLKGVVEAAGSSLNRVVKTTVFLADMNDFAAMNEVYSRYFTANAPA
                     RSTVEVARLPKNVRVEIDLIALL"
     gene            complement(5311..5925)
                     /gene="def"
                     /locus_tag="VG272_00500"
     CDS             complement(5311..5925)
                     /gene="def"
                     /locus_tag="VG272_00500"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWF12187.1"
                     /translation="MKIEIVQAGHPVLRQRGRQLSVAEIRSGEMQKLIEAMRTCMHEA
                     PGVGLAAPQLGLAIQLAVIEDREEYHKDVSEALLAERERRPVPFHAIINPILEEIGEE
                     KAEFFEGCLSVAGFSALVSRSRSVRVTCLDERGVSQVIEAKGWYARILQHEIDHLNGA
                     LYIDRMRTRSFTTMENLVEYWKGKPVSDIRSELEINPPVPATEQ"
     gene            complement(6048..6683)
                     /locus_tag="VG272_00505"
     CDS             complement(6048..6683)
                     /locus_tag="VG272_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12188.1"
                     /translation="MLPLQTWRHSARRLPCALLLAAIALLSSCKSAPKADTEQTNQMA
                     MWLDSVPELKTLGISNAEVNELKKAHEAGLTDPSSVLLIKLARDRHTPFTDGQPIADL
                     LNAGSSEGTVLQLARLNELGIWAGEARAMRLAGLSDKIILAVAQRRSQGLPVLSGEKL
                     AEVKNTGASDATILDFVQKGTSEKSASDYIAQRNRLSGHKFVYQGRSRKNG"
     gene            complement(6916..>7102)
                     /locus_tag="VG272_00510"
     CDS             complement(6916..>7102)
                     /locus_tag="VG272_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HWF12189.1"
                     /translation="DITAVISETGSNIRTLGSHPDNLHARIDLTLEISDRKQLERIIA
                     NIRKISGVFGVERLYNV"
BASE COUNT         1533 a         2064 c         1873 g         1632 t
ORIGIN      
        1 cggcgactca agttgcgctc gaaattcatc gaagccgcgt tcctcgtccg catggatcag
       61 acccggaccc acatacacca aaagcccgcg cctcgcaccg cccaaagctg cgcggcctgt
      121 ttctagagcg ctaggaatat ccgcggcaat gctcgagggc tgcacattct gaatcgctcg
      181 gcgaagtgcc gcgcggtccg cggtaaacgg caagatcggc ggcgcacccg cctcggcgcg
      241 aaggagcaga acgcggtcgc cgctcggaag cgagttcagc actcgcaggg cttcagcgcg
      301 ttcccgatca atccatgctg agtcccctgc gggatgcgcc tgcgaccaga tagatgcatc
      361 gagcacgagg gcaacgctgc gaccctcgaa tgcgggaccc cagcgcggat tcgccagcgc
      421 caaaataagc agcagtaaaa ataaaatctg cgcgagaaat gcccatggct cgcgaagctt
      481 gcgccggcgc ggttgcgaaa tcggctgtat gctggcccag aatctaagcg tggagacttt
      541 cctgtgctgt ggccgccggt ggtgcaaata aagccatagc gcaagagccg caagcccggc
      601 ccaaattgcc gcgacccaac cgggactgag cgccagccag ttcatgccag cacctgcccg
      661 ctacgcaagc cgcgcaaaac aaattcctgg tacgcaatat tagtaaccgc gcgcgcgtat
      721 cttccgccat tgcggatggc gaggcgccgc aatcgcgcgg aaaattcctg aaatctacgt
      781 tcgtagagcg cggcgctttg tggagaacat tctaccgtgc gatgcaatcc ggtctcggcg
      841 tcttccagaa ttaattcgcc gctggctgta ggatgctgct cctcggaact gtgtacctga
      901 agcaatacga aatcgtgccc ggcggaacga agcatctcaa ccgcgctctc gcagccttct
      961 tcgtcgaaaa aatcggaaac taaaatcaca attccacgcg acggaaaatt gcccagaaat
     1021 tcttttaccg acgttaatac gtctgtcgtt ccgccacatt tttgttcgct caggaattgc
     1081 agcacgggcc acagccggtc gcgtccgccg gaaacaacca gcggcttttt tagccgatcc
     1141 gcgaacggaa ttacggcagt ggtgtccaga tgcagcaacg ccaggtaagc aaaagttgcc
     1201 gccagtcgct gcgcgtaaag aaatttggac tcacccgcat cgccaaatcc catcgaatcg
     1261 ctgcagtcca tcaaaattcg cacggggatg tgctgctcgg tttcgtaaat tttcaggaaa
     1321 agttttccaa agcgaagata ggcgctccaa tccagcgagc ggggatcgtc gccaaagctg
     1381 tagcgccggt aatcggcgaa ttccattccc ggcccgcgag tccgggaacg atgctccggg
     1441 ccaaccgcgc cggacaaatt cgtgcggcat agcagcgtga cctgctccag cgttttcaga
     1501 aattcgcggt ccagcaagag cggcgaagtg gtagccattt gtttaatagg tagcgcgggc
     1561 ttcagtctgc ggggtttggt cctcgcaaag cctcaaccct tcgggctgaa gcccgcgcga
     1621 taacttcagc cggaaatgaa atttttattt cgccgaaatt tttccggcaa tttcttccag
     1681 caaatcgtca ggagcgcggc cgtccgcctg agcctcgaaa ttaagtatga tgcggtggcg
     1741 caaggccggg agcatcagcg cgcgaatatc ttccatgctc acgttgtagc gtccatccag
     1801 cagcgcacgc actttcccgc ccagaaccaa cgtctgcgcg ccgcgtggag aacttccata
     1861 ccggatatac ttgctcgcca aaacattttc gcgattctcc gggtgcgtcg ccagcgtcag
     1921 cttcaccaca tattcctgca cgtgtggcgc gacgggaacc gcgcgcacca ctcttcgcat
     1981 ctccagaatt tgcgcgccat ttaccacggg attgaccgtc gcgctcgttc cctgcgtcgt
     2041 gcgatccaaa atgtcgtgca ggtcttcgcg tccgggataa ggcacacgca gcttaaaaag
     2101 aaaccggtcc agttgcgcct caggcagcgg ataagttcct tccatttcca gcggattctg
     2161 cgtggccatt acgaaaaacg gttcgtcgag caaatgcgaa gtgtgggcca cggtcacttg
     2221 cttttcctgc atagcctcga gcagcgccga ttgtgttttc ggagttgccc gattaatttc
     2281 atcagcgaga agcaaattcg tgaagatggg tcccggctga aagccaagcg atttccttcc
     2341 atcgccattt tcctgcacca ccgatgttcc gatcacgtca gcaggcatca ggtcgggagt
     2401 aaactgaatg cgcgaatatt tcagcgcgac ggcctgcgcc agcgtgcgga ccagcatggt
     2461 tttccccagg cctggcgcgc cttccagcag cacatgcccg ccagccagca aagcaatcag
     2521 gacgccttcg acgacttcgg cctggcccac aatcgccttg gaaatttccc ggcgcacctc
     2581 cgccattttt tcggagagaa attccgcctg ctctccaaga ggttggctgc ccggctgccc
     2641 ttctgttccc accgaacttg tcatgaattc atccttgtgt tcaacttcga cttcatcttc
     2701 gcggcgtccg ctcgctccga acttcccgga ctcgcatggc atttccgggg aattctgact
     2761 caatgctttc cattatccgc atgctcgaaa tatcgctgca cataaaaccg gtagcgcgcc
     2821 gggatatttt cctgctccga accgttggtc accgcatcct gctgcggcgc ttgatcacgc
     2881 agggcaagct gtgccgtccc ggtttccgtg gccactttca tgcgtgtctg ctctttaaag
     2941 ccgctggatt ctagcgcaac acgatcagga acggcattca cgggatgcga ctcgaggtcc
     3001 gtgcgaagat ctttggttcc ctgctggctg ccggcgccct cgctggcggt ctgcgaggct
     3061 ttctgttttg cgtccgagct tccctttgaa tcgccgcgtc tgtcgctttc cgttgctcca
     3121 ggctggttgg aattcccgga ttgtggcgag ccctgcgaat tcggcggttg ctgattcgca
     3181 cgattattgt tggattgctg attcggcgat ttcgacatca tatttttcag agcctgcatc
     3241 agagactggc tcacggattg gcgcccctgc ggtcctgttt gttcggactg ctcctgcgat
     3301 ttcccatcgt tgctttcatt cccattcttg ctttcctgtg cctgcgaatt gctgtcctgt
     3361 ggacggccat cggccgaagg agttccctct tgatccccag gcggctgcat ttgatcctgc
     3421 ggctgatctc ctgcatccgc tgtatttttt tgttggtccg tgctgggaga tttttcgtca
     3481 gccttccaca aatcgtcagt cgcggcttcc tcggcacgtt tggtttcttc gccgagggtg
     3541 tccggcttcg cactggccag ggcaaacgtt cgctgaatat ctttttcgat cgcacgcgca
     3601 atgggagaaa ggattgattg gacaagctgc gaatgcgccg tagtctgcag aagtgaaatg
     3661 agaggtggct ggtgatgaac gcgataggca aataatccgg ccacgattaa gacaagtgcg
     3721 gcagcgcgct ttgcgtttcc cggccactcg aaaggaaata attcgcggcg gtccactttt
     3781 ccaaggcgtg agagcgcgtc cttgcgctgc gagccaacga ctccgtcagt atcctgcaca
     3841 gcacccaagt agatcgcggt ggaaagacgg tccttcaggc tagcggcaga atcgagccgc
     3901 cgagcgactt cgtaatgaga gggtcggtcc cgccaaatca cggcgagtat gccaatcgcg
     3961 accgtaagcg caagaatgcc cacttcaact attggcgcga atagcttgct gctgaccgct
     4021 gcaaccaccg cgaggacggc cagtatccag ctaaaccatt tcacccagcc ctgcagaatt
     4081 gtattggcca gaattctttg acgaatcggt ttgagagtgt cgattaggcc gttcaattca
     4141 ggcgagcggg cttggtggtc aaaaatcctg gcgagtagaa tttgattttc gcgggattgc
     4201 ccctgattca ttgcgaagaa tccccggtaa aaacctcgca acccagagaa agaggccctc
     4261 ccggttgact taccgggcac gaccaaatta attttctcgc gaaggagcat ggcggccctc
     4321 acagaggttc ggcctgggca tttccttgta caaatctacc ccaacattcc cgctttccat
     4381 actaaatccg tggcgataca ggatcaaact cgttggcctg atgcgctcaa tatccaaatc
     4441 cgatggacag ccgaacggaa cgacaaacgc gtcagtagat ttggatgccg ggcgcgaggt
     4501 gatggtggtg caacttccca ccggaagcag tttttcagaa tcaggaacgg ttccaatgac
     4561 ttcaggatgg aaattactaa gttccaatga ttctgtgcac tgcagggaat ttcagtccac
     4621 gtgtttccag gctcgactga ggattcagtt gcttagacac agagtgaaaa tcagatgcgt
     4681 tcaaaccggg aaagagcttg agaactatgt tcgcccgtat cgcagcaacg ctttcctacg
     4741 atcaggtgcg accacctccc aagttgcagc ccgaggcacg cggctccatc agtgcgctag
     4801 tcaaacaatg ccgcgcccaa ctcgtcccag cggcgccgac cttcgccctg cactgaaccg
     4861 tttcagattt ggaaacagac actcgcagga acatcaaagc agagcaatca ggtcaatttc
     4921 gacacgcacg tttttaggaa gccgtgccac ctcgacggtc gatcgcgcag gagcattcgc
     4981 ggtgaaatac cgggagtaca cttcattcat cgccgcaaaa tcgttcatgt cggccagaaa
     5041 cacggtagtt ttcaccacgc gattcaggga cgatcccgcc gcttccacta caccttttag
     5101 attttccatt acgcgttccg tctgaaccgc gatgtcgccc tccacgattt gctgcgtctt
     5161 cgggtccagc ggaatttgcc cggatagaaa tacaaagccg ttggcgcgca ctgcctgaga
     5221 atagggccca atggccttgg gccctcgatc cgttgcaacc gtttcgatca agcgatcctc
     5281 ctgagtgtga gagcctactt taatttcaaa tcattgctcc gttgcaggta cgggagggtt
     5341 gatttccaat tccgatctga tgtcactcac cggcttgccc ttccaatact ccaccaaatt
     5401 ttccattgtc gtgaaggatc tcgtcctcat gcgatcaata tagagcgcgc cattcaagtg
     5461 atcgatttcg tgctgcagaa tcctcgcata ccatccctta gcctcgatca cttgcgacac
     5521 tccccgctca tcgaggcagg tgacacgcac ggagcgcgat cgtgaaacca gtgcggaaaa
     5581 acccgcgacg ctgaggcatc cctcgaaaaa ctccgccttt tcttctccga tttcctcaag
     5641 aataggattg atgatcgcat gaaatggcac ggggcggcgt tcgcgctccg cgagcaacgc
     5701 ttcggaaacg tccttgtgat attcctcgcg gtcttcaatc acggcaagct gaatcgcgag
     5761 gccgagttga ggcgccgcca atcccacgcc gggcgcttca tgcatgcacg tacgcatcgc
     5821 ttcaatcagc ttttgcattt caccgctgcg aatttccgcg acgctcagtt gtcggccgcg
     5881 ctgccgcaac acgggatgcc ctgcctgaac gatttcaatt ttcatgggat tcgatatttc
     5941 cgcgtgcaaa ccggcgcatt gcaggcgctc ctgaatttaa tttcagcttt tgaggatata
     6001 cgtgaagagt ctcgctgcaa aatgaaatcg tcaaactgcg gcgatcgtca accgttcttt
     6061 cgagagcgcc cttgataaac aaatttgtgc ccggacagcc ggttgcgttg ggcaatataa
     6121 tcgctggcgg atttttcgga ggtgcctttt tgcacaaaat caagaatcgt tgcatcactt
     6181 gccccggtgt tttttacttc tgccagtttt tcaccggaaa gcacgggcag accctgcgat
     6241 ctgcgttgtg ctaccgccag aataatcttg tcggaaaggc ctgccaggcg catcgctcga
     6301 gcttctccgg cccagatgcc caactcgttc agccgcgcta gctgcaaaac cgtgccttcc
     6361 gacgatcctg cgttcaataa atcggcaatc ggttgcccat ccgtgaatgg cgtgtggcga
     6421 tcgcgcgcga gcttaatcaa taaaacactc gatggatcgg tcagtccggc ttcatgcgct
     6481 ttcttcagct cattcacttc cgcgttggat atccccagcg tctttaattc cggcacgctg
     6541 tccagccaca ttgccatctg gttggtttgt tcggtgtctg cttttggagc ggatttgcag
     6601 gagctaagta aagcgatggc cgccagcaac aatgcacacg gcaggcggcg agcgctatgg
     6661 cgccatgtct gtaaagggag catttgtcca ttgtaactta ggcgtgaagg accggaattt
     6721 gtgtgtgctt ggaagattaa ctggacatca ggcacaggta ggacaggctt cagagtctgt
     6781 gtgacaacta cagaatcttt gtgggtcgtg gcttcagcca cgacatacgc tctgcgaaat
     6841 gagtgcggct tcagccgctg aggttccaac gctacagttc tcacacagac tctgaggcct
     6901 gtcccgctaa aaccgtcaaa cgttgtacaa acgttcgact ccgaacactc ccgaaatttt
     6961 gcggatattc gcaatgatgc gttcgagctg tttgcggtcg ctaatttcca gtgtgagatc
     7021 gatacgggcg tgcaaattat ccggatggct gccaagcgta cgaatgtttg atcctgtctc
     7081 gctgattacg gcggtaatat cg
//
LOCUS       DASZMB010000014         4563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2212444, whole genome shotgun sequence.
ACCESSION   DASZMB010000014 DASZMB010000000
VERSION     DASZMB010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2212444"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2185
                     /gene="lon"
                     /locus_tag="VG272_00515"
     CDS             <1..2185
                     /gene="lon"
                     /locus_tag="VG272_00515"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HWF12190.1"
                     /translation="LVRMPNGNVVVFLEGLQRIRVMELVTFRPFLTAHVEIQPDIIGE
                     EDAELMALERNARELFRDVVARSPQLSDDLQTAALNIEDPVRLADFIAANLPSLPTLL
                     RQELLETADVRRRLETLIRELSKELEVLELRNKIQEQVQEQVSQSQREYLLREQLKAI
                     HKELGESDDGQAEIDELREKLEKSGMSAEARKECDREIKRLAKMTPASAEYMVARTYL
                     EWMISLPWTKSSGVEEIDIAKGQAILDEDHYDLEKVKERILDYLAVKKLKPGMKGPIL
                     CFLGPPGVGKTSLGKSIARALGRKFVRISMGGMHDEAEIRGHRRTYIGALPGQIIQGL
                     RRAETNDPVFMLDEVDKLGRDFRGDPSAALMEVLDPEQNNAFRDHYLDVPFDLSKVLF
                     IATANMIDPIPEPLRDRMEIIELAGYIEMEKIQIAHKYLVPKQAGEHGLKIGEQIEFT
                     DDGLREIVHSYTREAGVRNLEREVATVIRKEARRIAEGKTEKLVVTPEVVHEALGVPK
                     FRAEREVEERVKTPGVSVGLVWTPAGGDIVFIEASRMRGGKQFTMTGHLGEVMQESMT
                     AALTWVRANAGRYDIDPDFFRKQDIHIHVPSGAVPKDGPSAGVAMVNALVSLLSGRAL
                     RPRVAMTGEISLSGIVLPVGGIKEKVLGAKRAGIREVILPAENEPNVKEDLQPHMLEG
                     MQIHYVRTVDQALDIALSKPVAAAAGLKTGQIPPVGPAPVVPPIH"
     gene            2354..2938
                     /locus_tag="VG272_00520"
     CDS             2354..2938
                     /locus_tag="VG272_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12191.1"
                     /translation="MRKLFPIYGSSLANNMLATLVHDRIVNGFVTTYVSQYDRKGILG
                     GPARDRELAETIGREIVLALIVEVESLMPSFFGKKQKKKLKADEKQAIDLFLRELRAA
                     LERAHNWNSEDRMQFHRDLTMYTDFDVRKELAASVRKKGKAQEEEAPFVARVALLLDP
                     SMLEQARRAARKFHGSMSVLARKLLRQTLSPGRR"
     gene            complement(3395..3739)
                     /locus_tag="VG272_00525"
     CDS             complement(3395..3739)
                     /locus_tag="VG272_00525"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HWF12192.1"
                     /translation="MPVQVATMMVTTSQELPGYRIVRNFGIVRGIIVRSRSLLGSIGA
                     GLQTIIGGNITILTELCEKTREEAYELMMQHAAQHGANAVIAMRYDATEIMQGVTEVL
                     AYGTAVQVEKIS"
     gene            3860..>4563
                     /locus_tag="VG272_00530"
     CDS             3860..>4563
                     /locus_tag="VG272_00530"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF12193.1"
                     /translation="MLALFYFLVIEQILQGLYNLWDGLVWLRLAQRRAGTHSGFYAPR
                     VALFCPVKGAEPGLELNISALVSFDYPDYEIFFSMASADDPARKIIERATAGSKHKIQ
                     IVIAGRPVECGEKVNNLRAAILKATEGFDVYVFADSDGRPGRSWLSRLVAPLADSNLG
                     AVTTFRWFFPQTGGFCSALASAWNAPAATYLGDHARNFCWGGGTAIRRERFEQCNVLE
                     FWNGSVSDDFSLTRALR"
BASE COUNT         1040 a         1168 c         1351 g         1004 t
ORIGIN      
        1 attggtgcgc atgccaaatg ggaatgtcgt cgtgttcctg gaaggattgc agcgcattcg
       61 tgtcatggag ctggttactt tccgtccgtt tttgacggca catgttgaaa ttcagcctga
      121 cattatcgga gaagaggacg ccgaattaat ggccctcgaa cgcaatgccc gtgagctgtt
      181 cagggacgtt gtcgcgcgtt caccgcaact ttccgacgac ttgcagaccg cggctctcaa
      241 catcgaagat ccggttcgcc tcgcggactt tattgccgcg aacttgcctt cgcttccgac
      301 attgctgcgc caggaacttc tggagacggc tgatgtgcgc cgccgcctgg aaacgctgat
      361 tcgagagctt tccaaggaac tggaagtttt ggagctgcgc aacaaaattc aggagcaagt
      421 gcaggagcag gtcagccaga gccagcgtga atatttgctg cgcgagcagt tgaaagccat
      481 tcacaaggaa ctgggcgagt ccgacgacgg gcaagcggaa atcgacgagc tgcgcgaaaa
      541 actcgagaaa tccggaatgt ccgctgaggc acgcaaggaa tgcgaccgcg aaatcaagcg
      601 gctcgcgaaa atgactccgg cttcggccga gtatatggtt gctcgcactt acctggaatg
      661 gatgatctcg cttccgtgga caaaatcctc gggcgtggaa gagatcgaca tcgccaaggg
      721 gcaagccata ttggacgagg atcactacga tctggaaaaa gtgaaagagc gcatcctcga
      781 ctatctggct gtgaaaaagc tgaaaccggg catgaagggg cctatcctgt gcttccttgg
      841 acctccagga gtgggaaaaa cttcgctcgg taaatcgatc gcgcgggcct tgggccgcaa
      901 attcgtgcgt atctcgatgg gagggatgca cgatgaagcg gaaattcgcg gacaccgccg
      961 gacgtatatc ggcgcgttgc ccggccagat cattcaagga cttcgccggg cggaaaccaa
     1021 cgatccggtt ttcatgctgg acgaagtgga caaactggga cgcgatttcc ggggcgatcc
     1081 atcggcagcg ctgatggaag tgcttgatcc ggaacaaaat aatgcgttcc gcgatcacta
     1141 cctggatgtg ccgttcgatc tgtccaaagt gctgttcatc gccacggcaa acatgatcga
     1201 tccgattccc gagccgctgc gagatcgtat ggaaatcatc gaactcgcgg gctatatcga
     1261 aatggaaaag atccagattg cccacaaata cctggttccg aaacaggcgg gagagcatgg
     1321 gctcaagatt ggcgaacaga tcgagtttac ggatgacggc ttgcgcgaaa ttgttcacag
     1381 ctacacgcgt gaagctggcg tgcgaaatct cgaacgtgaa gtagccacgg taatccgaaa
     1441 agaggctcgg cgcattgccg agggcaaaac cgagaagctg gtggttacgc ccgaggttgt
     1501 gcacgaagct cttggagtgc cgaagttccg tgccgaacgg gaagttgaag agcgtgtcaa
     1561 gacgccggga gtttccgttg gactggtctg gacgcctgca ggcggcgaca ttgtcttcat
     1621 cgaggcaagc cgcatgagag gcggaaagca gttcacgatg acggggcatc tgggcgaagt
     1681 gatgcaggag tcgatgacgg ccgcgttgac gtgggttcga gccaatgccg gacgctatga
     1741 catcgatccc gactttttcc ggaagcagga catccacatc cacgtgccgt caggcgcagt
     1801 gccaaaggat ggcccatcag ccggcgtcgc aatggtcaac gcgctggtga gtttgctctc
     1861 tggccgcgcg cttcgtccgc gcgttgcaat gacgggcgaa atttcgctct ccggcattgt
     1921 tttgccggtg ggcggaatca aggaaaaggt gttgggagcg aaacgcgccg gaatccgcga
     1981 ggtgattttg cccgcggaga acgagccgaa tgtgaaggag gacctgcagc cacacatgct
     2041 cgaaggaatg caaattcact acgtgcgtac ggtggatcag gctctggaca tcgcgctctc
     2101 gaagccagtg gctgccgcag ctggtttgaa aactggccag attccaccgg tcggaccggc
     2161 gcccgtggta ccaccgattc attagctgat cacaatcaga agggaaaagg cccggctcca
     2221 gcaaacggag ccgggctttt tttgtggcat aggtttcagc ctgtcgggtt tggtccttgc
     2281 aaatcctaaa ttccacaggc tgaagcctat gccagaagaa ccttcctgct gctaatatct
     2341 ggcgatggcg cgtgtgcgca aattgtttcc aatctatgga agctcgctcg cgaacaatat
     2401 gctcgcgacg cttgtgcatg accgcattgt gaacggcttt gtcaccacct atgtatcgca
     2461 gtacgaccga aaagggattc tcggcgggcc cgcgcgcgac agggaattgg ctgagacgat
     2521 tggacgtgag atcgtactgg cgttgattgt ggaagtggaa agcttgatgc cttccttttt
     2581 cggcaagaaa caaaagaaaa aactgaaggc ggatgaaaag caggccatcg atttgttttt
     2641 gcgcgaactg cgagcggcgc tggagcgcgc gcacaactgg aatagtgagg atcgcatgca
     2701 gtttcaccgc gacctgacga tgtatacgga ttttgatgtg cgcaaggagc ttgccgccag
     2761 cgtacgcaag aaagggaagg cacaggagga agaggcgccg ttcgtcgcgc gcgtagcgct
     2821 gcttctcgat ccatcgatgc tggagcaggc gcgtcgcgcg gcgcgaaaat ttcacggaag
     2881 catgagcgtg ttagctcgaa aattattgcg gcagactttg agtcctgggc gcaggtgatt
     2941 gcctggggga aaaatgaatt gcagattaca gacagggaca actttgaatc gaattttccg
     3001 cgaaaattag aagccattca taacgtttca tggatggctt ttgtgggtcg cggctgaagc
     3061 cgcgacattc acggactcaa aaatcaaggg cttcagcccc tgaagtccta ccatgttgct
     3121 gcggcgatcc aattccgaaa tggctcggct ctgtcaacat ccccagttcc gctgccgatc
     3181 tgctgtagcc cgtctcttca gaggcggggt tttagacgat gaccgactcg aagcctgaaa
     3241 taagggcgcc gaaggtcggg ccgcgacctg aaacccaggc acgcgcgctg aacaggaaat
     3301 tttgggtcgc actttcatct aaaccttcgc ctctcaagag gcgagctaca aatccggcgt
     3361 cagcagagac agatagcttt ccaattagaa gtagttaaga aattttttcc acttgcacgg
     3421 cggtgccgta ggcaaggact tccgtaacgc cctgcataat ttccgtcgca tcgtaacgca
     3481 ttgcaatgac ggcgttggcc ccgtgctgag ccgcatgctg catcatcagt tcgtaagcct
     3541 cttcgcgcgt tttttcgcag agttcggtca gaatcgtaat attgcctccg ataatcgttt
     3601 gcagtccggc gccaatggag ccaagcaggc tgcgcgaacg cacaatgata ccgcgcacga
     3661 ttccgaaatt gcgcacgatg cggtagcctg gcaattcctg ggaagtggtt accatcatcg
     3721 ttgcaacttg aacaggcatg gaaatttctc cttatgtacg gattggtttc aaagcgatac
     3781 taacggtcct ttgcgctggc cactcctctg ttatgctccg gcctgtacct tttagttatg
     3841 atccgccaaa aggcaggcta tgcttgcgct gttctatttt ctggtcatcg aacagatttt
     3901 gcagggcttg tacaatcttt gggatgggct ggtttggctt cgattggcgc agcgccgggc
     3961 cggaactcac tcaggatttt atgcgcctcg cgttgcattg ttttgtcccg tgaagggcgc
     4021 ggagcctgga ctggaactaa atatttcagc gctcgtttct ttcgactacc ccgattacga
     4081 gattttcttc agcatggcaa gcgcggacga tcccgcgcgc aaaatcatcg aacgggccac
     4141 tgctgggagc aaacataaaa ttcaaattgt aattgccggt cgaccggtcg agtgcggaga
     4201 aaaagtgaac aatcttcgcg cggcgatcct gaaggcaacc gaaggtttcg acgtttatgt
     4261 gttcgcagat tctgacgggc gtcccggcag aagttggctc tcgcgtctgg tcgcgcctct
     4321 tgcggattcc aatctcggcg cggtgactac gtttcgctgg ttttttccgc agaccggcgg
     4381 attctgtagc gctctggcat cggcctggaa tgcccctgcg gcaacgtacc tcggcgatca
     4441 cgcgcgaaat ttttgttggg gcggtggcac cgccattcgc agggaacgct ttgaacagtg
     4501 caacgtgctt gaattctgga atggttccgt gagcgatgac ttttcgctga cgcgcgcgct
     4561 gcg
//
LOCUS       DASZMB010000015        21477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1397040, whole genome shotgun sequence.
ACCESSION   DASZMB010000015 DASZMB010000000
VERSION     DASZMB010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 21477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21477
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1397040"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /locus_tag="VG272_00535"
     CDS             <1..1410
                     /locus_tag="VG272_00535"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HWF12194.1"
                     /translation="KRRIMAVDVIMPQMGESIFEGTITRWLKKPGDRVERDEPLFEIS
                     TDKVDAEIPAPSAGILKEIKVTEGKTVPIQTVVAVIDAAGSEAAAPTVAPAAPTPAPE
                     KGAAPKAAAPQAAPAPSAPAPAPVAPQAASAPPVAPAVRANGQKIRSSPLVRKIAKEH
                     NVDLASMEGSGAGGRISKKDILEALETGGTKPAPSAPAPAAPEPAGQQASATPAPPPG
                     TGGALAHPVLENAVPRERIYFGKYEAQPMSMMRQRIAEHMVASKHVSPHVYSIDEADM
                     TRIAKIRERSKSAFEAKYGTKLTYMPFFVRACVEALRAFPTVNASCDGATVVLHREIN
                     VGIAVALDWGLIVPVLKNAEEKNFLGLARGMNDLAERARAKKLKPEEVQEGTFSITNP
                     GVYGGLMGLPVINQPNVAILGIGAIEKRPMVIDDAIAVRSMVYLTLSYDHRVVDGAVA
                     HQFVGKVKSFLEAWNEDIL"
     gene            complement(1633..2454)
                     /locus_tag="VG272_00540"
     CDS             complement(1633..2454)
                     /locus_tag="VG272_00540"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HWF12195.1"
                     /translation="MPDETLQPCAYHVVRYQPNLIRDEWVNIGVLLLDPATGRVRQRW
                     LEEPADLARLRRMHPAADEELLLRLPAEFYRQFAGLEMDSESLLRKFDETLSNAIQLA
                     PRKGLLARDPDLELDRVFREQVEPLREPRRGVVEIRTRNDVRAQAADYFRTEKILRLM
                     QRSVRVEEFTAPGDPMRIDFSYRRNGTRGFVHSVALGRDPGQAKLLAYTAKAIRDHVK
                     HTEFLAVTERELDRGNRRDQFVSGILLDSNIRVVPLAQLRKWAHDVAPVLKAGNL"
     gene            complement(2447..3337)
                     /locus_tag="VG272_00545"
     CDS             complement(2447..3337)
                     /locus_tag="VG272_00545"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HWF12196.1"
                     /translation="MAVMKLAIEHVRRMRGGAQAHLMRCEDDAYYIVKFQNNPQHLKI
                     LANEMLATRLAARLGLCVPQVEVVEVRPEIIAYTPDLVMQLGLGRKPCAAGKQFGSQF
                     PGHPAHMTVHDFLPDEQLRCVRNLSDFLGVLVLDKWTCNTNGRQAIFFRDPEELAVAA
                     GNFLPDDAAATSMVNTYTSAGYTAMMIDFGFCFNAGEWDFPDAPLRGLYMRQRVYQGV
                     DGMCAFEPWLSRLENQFTERVLGEESAQIPPEWYGGDWNAMEQLIERLYRRRDRVREL
                     LLSARNSGRDPFPNWKVKHA"
     gene            complement(3520..3849)
                     /locus_tag="VG272_00550"
     CDS             complement(3520..3849)
                     /locus_tag="VG272_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12197.1"
                     /translation="MAYEKEDQNDENEFVHFEEEELGGDPEDVVEEETEEEVVITERP
                     DMAAVSEPASTPKPAAKKKAPAKKAKKPAPKKKAKVKAKPAPKKAAKKASKKKPANKA
                     AKRGKKR"
     gene            complement(4093..6045)
                     /locus_tag="VG272_00555"
     CDS             complement(4093..6045)
                     /locus_tag="VG272_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWF12198.1"
                     /translation="MGCRAQKSAAWIFVAAGVLAGLVGCDDSSKKLVQVKPPQITLPV
                     QQPVQTAAPVETQTKIAPPVVAPAPSGPEQVATESQTAFDAGEQDFKAGHLGKAREEF
                     DAALDHLLASGFDLDSDPRLSGLYHHIIDTVSLDELEAFRAGDGFSEQKSTPAPIDEI
                     AEQPIPQPEKFDPNLRGRAEGEVGAIDHDLPLTVNDPVLAYLNYFKTPRGSAIVETGL
                     RRAGRYREMVRRVLKEEGMPQDLIYLAQAESAFLPQAVSKAGARGMWQFMSFAGRKYG
                     LQKTWWVDERQDPEKATRAAARDLRDLYDQFGDWYLAMAAYNSGAGAVQHAVERTGYA
                     DFWELYHRNALPKETMNYVPIILALTLISKDPARYGIEFEPEPALKADKVKPGQAIDL
                     RLVAETIDTDLESLRSMNPELLRLVTPPDPDFVLQIPEGSAEHFFAEIAAIPPEKWVS
                     WRRHKVEQGETLSSIAKQYRVSTAEVADANELALGTQLEEGQKLIIPAAARTEASSGK
                     LIRYRVRRTDTIATIADEFDVTAAELRKWNHLKADRVVRGTSLRIYPGGMTPGPAQQQ
                     EASNKTKTAVPTPVAARQSASTRKSPVMEARASNSVVHRVKQGETLWSIARAYQTTVE
                     AIQAGNRYLFSRPLQVGDTLTILQTH"
     gene            complement(6256..7437)
                     /locus_tag="VG272_00560"
     CDS             complement(6256..7437)
                     /locus_tag="VG272_00560"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWF12199.1"
                     /translation="MPKKTSPQWSDATLAIHSGEPKHGLNASVTAPIVRSANFTFADT
                     AEMKRWAEGKSKAYLYTRYGNPTLAVAEEKIAKLENGEAALVTASGSASISSALLSVL
                     KAGDELIATRQLYGGSYRLMRDILPRMGIRVHHVEANLDGFDRLVNAKTRAFYVETPT
                     NPTLGLVDLKRSAALARKHKLVSIIDNTFATPILQKPLNFGFDLVVHSATKYLAGHSD
                     IIAGAVAGRRERVGKVREMMISLGGSMDPDPAYLLIRGLKTLEIRVRRQCQSAMAVAR
                     FLERHPKIQRVHYPGLPSHPHHRLAKQQMKGFGGMLAFDMKGGLPAARRFCDKSEIFL
                     LAASLGGVESLIVLPIYTTHFGLAAPELAAAGVGKGTVRMSVGLEEASDLIADLKHAL
                     S"
     gene            complement(7591..9759)
                     /locus_tag="VG272_00565"
     CDS             complement(7591..9759)
                     /locus_tag="VG272_00565"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWF12200.1"
                     /translation="MRAYETQSIRNVGIAGHGDTGKTQLVSSLLFTAGMTTRLGKVSE
                     GNTVTDWDDEEIARKITIHTSLAYAEWTPSGQFGKVDQPGKSDRSEKIKINFLDTPGY
                     STFLNDTRASLVAADALLIVVDAVAGVQVVTEKAWDYGVEYDVPRAFCINWMDRELAN
                     HDRAMESITRVFGRNVVPLQLPIGSERGFRGVVDLITMKAHLYTPDGDGKPTIEEIPA
                     VLAEASKTAHETLVEMVAEGDDALMEEFFEKGTLPIEDLRKGLREAFQAKRIYPVVLT
                     SALHNIGSATLLDLLADVFPDPSQRGTAIGHSETASKGNEMKRKIADAEALSIFVFKT
                     LADPFAGRVTYFKVMSGVLKNDAMPQNFTRGTSERLQHIQVMQGKTATGVPELHAGDL
                     GTVAKLKETFTGDTLGDKSAPIHYPLPHVPEPSITFAVEPKTRADEDRIGPAIHKILE
                     EDPSLRFSRDPQTKEFLLAGSGQQHIEVVVAKLHKRYHVDLKLHPPKVPYRETIRGKA
                     DAEGKHKKQTGGHGQFGVCRVRLEPAARGTGIEFIDDIFGGAIPKNWIPSVEKGIRAS
                     AERGYLAGFPVVDFKAILHDGKYHDVDSSDMAFKIAGSLAFKECMKQARPTLLEPVMH
                     VEVFAPDQYSGDIMGDLSSRRGRINGSEVSGATVIVKASVPFAEMLNYATTLTSMTQG
                     RATYTMEYSHYDYVPAEIAEKVIAAAKAARGVEVEEEEHV"
     gene            10094..10627
                     /locus_tag="VG272_00570"
     CDS             10094..10627
                     /locus_tag="VG272_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12201.1"
                     /translation="MLALEVVTVFLAAIAMSMALAHALEYPGKRRLDAETYMAVQTIY
                     YPGFTLGGIGEPLAAISTLLLLIAVRDRGVVFWLILGALFALVAMHLVFWFVTQPTNK
                     FWLRNQQLSGAGARFFNVDRSEAGSTEVDRANPAMLREKVNPEWKRFRDRWEYSHVIR
                     AALSAMALIALIIAVAM"
     gene            complement(10813..11856)
                     /gene="purM"
                     /locus_tag="VG272_00575"
     CDS             complement(10813..11856)
                     /gene="purM"
                     /locus_tag="VG272_00575"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HWF12202.1"
                     /translation="MDYTEAGVDISLADQAKERIRRLASRTFTRGVIGGIGSFGGLFA
                     LDKKWKEPVLVSSADGVGTKLKIAMAMNIHSTVGADLVNHCVNDILTLGAEPLFFLDY
                     LAMSKMDPNVVEQLVEGMSRACHTAGCALIGGETAEMPGFYAPGEYDLAGFIVGVTER
                     ASLKKTNVVKPGDLLFALPSTGLHTNGYSLARKLIFDHAGLHPESYVAEVNNKIGAEL
                     LRPHRCYWPMLKTTVAKGWPSAIAHITGGGIPGNLPRVLPRGVQAEIELGSWPVPSIF
                     TYLAGLGKLERDDLLRTFNMGVGMILVVPIENHRTLETELKRRREKYFRIGQIRRCDP
                     RKPQVVFTGNFPQ"
     gene            complement(11948..12637)
                     /gene="lepB"
                     /locus_tag="VG272_00580"
     CDS             complement(11948..12637)
                     /gene="lepB"
                     /locus_tag="VG272_00580"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWF12203.1"
                     /translation="MNAEETIPVVVQPKPDINKIILREALSWLWVILAFLFITGTIVQ
                     ARVIPSGSMERTLLVGDHLLMSRFGYEAELPMTPLHMRLWRDPRRQQIIVFRAPLPGT
                     PDYIKRLIGLPGDQLEIREGVVSVNGKPLKEPYRADPPNPTDNYGPVTVPAGEYFMMG
                     DNRDDSYDSRYWGFVPRENIIGTPIMVYMSIDAPEDTWNPGHVRDRMTAYASILLHPS
                     IVRWRRIFKLF"
     gene            complement(12627..14177)
                     /gene="purF"
                     /locus_tag="VG272_00585"
     CDS             complement(12627..14177)
                     /gene="purF"
                     /locus_tag="VG272_00585"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HWF12204.1"
                     /translation="MNRLIEIENDRFHDHCGVCGVFGHPEASKLAYLGLYALQHRGQE
                     SAGIVSSDGRELYLEKSMGLVADIFQPHVLARLPGEAALGHTRYSTAGDTSLMNAQPI
                     VIDCNKGKLALGHNGNLPSAARLRRTLEHRGSIFQTTSDTEVIVHLIARSQARNLSSA
                     LADALNQVEGAYSLLVLTRDEMYALRDPRGFRPLALGQLDGAWVVASETCAFDLIGAT
                     YVRDIEPGEMLRISRAGIESLRFAAPKPLQQCIFEHVYFARPDSLVFGRVVEQSREML
                     GRMLAREHHVPADIVVPVPDSGVPSAVGYSQESGIPFRMGLIRNHYVGRTFIEPQQSI
                     RDFGVKLKLNPVPELLRGKRVVLVDDSIVRGTTSRKIVRMVREAGATEVHMRISCPPT
                     ISPCYYGVDTPTREELIASDESRVRPRLTAEELTAFEAKLDQGSLPPDREQRSIEEIR
                     QYLGADTLGYVSLSNLKVAVDDTRGSFCTSCYTGVYPVDGAQAELESRTMEIDVPAVE
                     IQVMPNER"
     gene            complement(14174..16555)
                     /gene="purL"
                     /locus_tag="VG272_00590"
     CDS             complement(14174..16555)
                     /gene="purL"
                     /locus_tag="VG272_00590"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HWF12205.1"
                     /translation="MLDTEIKVTPQLAAEHGLTADEYGRIQKILGRDPNITELGIFSV
                     MWSEHCSYKSSKVHLRRLPTRGPQVLQGPGENAGVVDIGDGLAAAFKMESHNHPSYVE
                     PFQGAATGVGGILRDIFTMGARPIAVLDSLRFGPISANVSGAGETGFSPSGQVTTKGA
                     LAPQPADRIDAETIAHNRRILDGVVRGISHYGNCFGVPTVGGEVQFEAGYSANPLVNV
                     LALGIAKKEDLFFAKARGVGNPVIYVGAKTGRDGIHGASLLASAEFTEESQQKRPNVQ
                     VGDPFMEKLLLEACLEAMKTGAIVAIQDMGAAGLTSTSCEMASRGGLGIEMELTRVPQ
                     REPGMTPYEMMLSESQERMLLVAARGREQEVLNVFAKWGLDAVEIGRVTEDGFVRVLH
                     HAKVVAEIPAHALAEEGPVYQRPLAAPAPVTNEHLVAFAEPGKNLTENFRALLASPAI
                     ASKRYIWEQYDYMVRTNTIEAPGAGDAAVVRIKGTKRALALASDGNGRWCRLDPFVGA
                     QLAVAEAARNVACSGAKPMAATNCLNFGSPEKPEVMWQFSRAIDGISEACIALEIPIT
                     GGNVSFYNETLGRSIDPTPVLGVLGMIENAPRALGMAFRATGDAILLLDGIASASSSD
                     ENAAKEFSSSEYARTTQGVEAGAPPAINLPAEKRLIALLVALAQEGQLQSAHDVSDGG
                     LAVTLAESCFTSNGLSAQISLTSKEPDELAFFGERGARAVVSVTPENVAAVRRIAAQY
                     EVAAVEVGRVASDEFRIELNGQMIVSAEVPSLADAWSGALGRLLNANDLRKAE"
     gene            complement(16693..17403)
                     /gene="purQ"
                     /locus_tag="VG272_00595"
     CDS             complement(16693..17403)
                     /gene="purQ"
                     /locus_tag="VG272_00595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HWF12206.1"
                     /translation="MKFGVVVFPGSNCDHDAWYALGEVLHQPVEFLWHQSEEVNNFDA
                     ILLPGGFSYGDYLRTGAIARFSPVMRAVEKFAHGGGLVIGICNGFQILCEAGLLPGVL
                     RRNAGMKFICRQVHIRVESTDTPFTGAADPGQVLQIPIAHGDGNYFCDEATLSELQRN
                     EQIIFRYSTPDGRIDESANPNGSLANIAGICNRERNVVGLMPHPERAVESALGSTDGQ
                     VIFRSLIESLLIQAGVSA"
     gene            complement(17541..17828)
                     /locus_tag="VG272_00600"
     CDS             complement(17541..17828)
                     /locus_tag="VG272_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12207.1"
                     /translation="MIRISKIVFAPLAMLLLLATGPVHLMAQDSTKDSTKDSAKQDTK
                     EAGQDTKDAAKATGRATKKTAKKTGHAVKKGTNKAADKTEEGAQKAKDKTE"
     gene            complement(18069..18320)
                     /locus_tag="VG272_00605"
     CDS             complement(18069..18320)
                     /locus_tag="VG272_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12208.1"
                     /translation="MGVWVNWGLVVAAIAFIFGDLVLEVYESVNLAVLGMFLLAGGIL
                     GLAVDSVAVGLATPVVLELVNLAIHRRPGQMLPKHVSGH"
     gene            complement(18580..20022)
                     /gene="glmU"
                     /locus_tag="VG272_00610"
     CDS             complement(18580..20022)
                     /gene="glmU"
                     /locus_tag="VG272_00610"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HWF12209.1"
                     /translation="MANSDFVIVVLSAGKGTRLKSALAKVLHRAGGRPLVEHVVRACQ
                     PIKPREIVAVVGYQAEDVTAAVAPLNVKTILQEPQCGTGHAMLVTRRAIPTRAKFVII
                     LPGDAPLIRTVTIAALARSHRESGAAATILSAEIENPSGYGRIVRQDDGSVSAIVEDS
                     ALTPEQRLIREINSSIYCFTLAKLWPCLSRLKPKNVHKELYLTDAIAVLREKGEKVQA
                     VLASDPDEVLGCNTRADLAEVDSVFRKRKRAAIMDAGVTIELPDTVLIDPEVMVGADT
                     RIEPGVQLLGKTRVGAGCTIRTGSVLSDAVLADNVLVKPYTMVTASHLSRGTQVGPFA
                     HIRESARLEENARVGNFVEVKKSVLGEGVKSMHLTYLGDARIGSKTNIGAGTITCNYD
                     GVNKNPTTIGKGVFIGSDTALVAPVRVGDGAYVGAGSVITKDVPADALGLARGKQMNK
                     LGWAASRRKEMAAAKSKKLGTGKKAKRKSK"
     gene            complement(20144..21070)
                     /gene="lpxC"
                     /locus_tag="VG272_00615"
     CDS             complement(20144..21070)
                     /gene="lpxC"
                     /locus_tag="VG272_00615"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HWF12210.1"
                     /translation="MLAYSADMSFQTTIERPVETSGIGLHTAVKSKLRLVPAPANTGI
                     VFRRVDLDNFEIEAHVRNVARVSYATSLMKKGVLLSTTEHLLAALYSCDVDNVYIEID
                     ALELPILDGSSQPFIEMLGQAGIRKLRKRRRYLKILKPLEFSDAGRRIGIYPADDFSV
                     RCYVEYAHKAVGAQEIELRVSRESFSRVLAPARTFGFVQDFEGLRRMGLIRGGSLENA
                     IVLNEDGVMNGPLRFPDEFGRHKALDLIGDLALVGRPLLARVEAYKAGHALHTQLVTR
                     LLADPTLWTETYDEAPAETPQHFAEAPATAGD"
BASE COUNT         4514 a         6070 c         6047 g         4846 t
ORIGIN      
        1 aagaggagaa tcatggccgt cgatgtcatc atgccccaga tgggggaaag cattttcgaa
       61 ggaacgatta ccaggtggct caagaagccg ggagaccggg tagagcgcga tgagccgttg
      121 tttgaaatct cgaccgacaa ggtcgacgcg gaaattcccg cgccttccgc cggaattctg
      181 aaggagatca aggtcacgga aggaaaaacc gtgcccattc agacggtggt cgcggtgatc
      241 gacgcggcgg gctcggaagc tgccgctccc acggtggcgc cggcggcgcc aactcctgcg
      301 ccagagaagg gtgcagcacc caaggccgcg gccccgcaag ccgcgccggc accttctgcc
      361 ccagcgccag cccctgtagc gccacaggcg gccagcgcgc ctcccgttgc gcctgcggtg
      421 cgcgccaatg gccaaaagat tcgcagttcg cccctcgttc ggaaaatcgc caaggaacac
      481 aatgtggacc tcgcgtccat ggaaggcagc ggtgcgggcg gccgcataag caagaaagat
      541 attctcgaag cgctggaaac gggcggcacg aaaccggccc cttctgcacc cgctcctgcc
      601 gcgcctgagc ctgccggtca gcaagcgtca gctactcctg cgccgccacc gggcacgggg
      661 ggagcactcg ctcatcccgt gctggaaaac gccgtgccgc gcgagcgcat ttacttcggc
      721 aagtacgaag cgcagcccat gagcatgatg cgtcagcgca ttgctgagca catggtggcc
      781 tcgaagcatg tttcgccgca cgtctattcc atcgacgagg cggacatgac gcgcatcgcc
      841 aaaatccgcg agcgttcgaa atccgcgttc gaagcgaagt acggcacgaa gcttacctac
      901 atgccgttct tcgtccgcgc ctgcgtcgag gcattgcggg catttccgac ggtcaatgcg
      961 tcctgcgacg gagcgacggt tgtgctgcat cgcgaaatca acgtgggcat cgccgtggcc
     1021 ttggattggg gcttgatcgt gcccgtgctg aaaaatgccg aggagaaaaa tttcctgggc
     1081 cttgcgcgcg gcatgaacga cttggcggaa cgcgcacgcg ccaaaaaact gaagcccgaa
     1141 gaagttcagg agggcacctt ctccatcacg aatcccggtg tgtatggcgg gttgatgggg
     1201 cttcccgtga tcaaccagcc gaacgttgcc atcctgggga tcggcgcgat cgagaagcgc
     1261 cccatggtga tcgacgatgc catcgccgtt cggtccatgg tgtatttgac gttgtcctac
     1321 gatcatcgcg tggtcgacgg agccgtagcg caccagtttg tcggcaaggt gaaatcgttc
     1381 ctggaagcgt ggaatgagga cattctgtag ctgcgtgatt cgtgatttgt gaattgtgac
     1441 tcgtgaaaaa aaggagctac tttccgtcag accgggaatt ggctcctttt ttatcgtacg
     1501 agggaacgcg caaggtgcag aaattagttg actcgaaatc gaacccgtag ttgactcacc
     1561 actcaccact caccactcac cactcaccag tcaccagtca ccagtcacga attacaaatc
     1621 acgattcaca aatcacaaat tccccgcctt caagaccggc gccacgtcat gcgcccactt
     1681 gcgaagctgt gcgagcggga cgacgcgaat attgctgtcc agcaaaatcc cggaaacaaa
     1741 ttgatcccga cggttgccgc ggtcgagttc gcgttcggtg accgccagga attcggtgtg
     1801 ttttacatgg tcacgaatcg cttttgccgt ataggcgaga agcttggcct ggccgggatc
     1861 gcggccgagc gccacggagt ggacaaatcc tcgcgtgccg ttgcggcggt acgaaaaatc
     1921 gatgcgcatg ggatcgccag gggccgtaaa ttcctcgacg cgcacgctgc gctgcatcag
     1981 gcgcaggatt ttttcggtac gaaaataatc cgcggcctgc gcgcgcacgt cgttgcgtgt
     2041 gcgaatctcg accacgccgc ggcgcggctc gcgcagcggt tcaacctgtt cccgaaagac
     2101 acggtccagt tcaaggtcag ggtcgcgggc cagcaaaccc ttgcgcggtg cgagttggat
     2161 agcgttggaa agagtctcat cgaattttcg cagaaggctt tctgaatcca tttcgagtcc
     2221 ggcaaactgc cggtaaaatt ccgcgggcag gcgcagcagc agctcttcgt ccgctgccgg
     2281 atgcatgcgg cgcaggcgcg cgagatcggc aggctcctcc agccagcgct ggcggacgcg
     2341 gcccgttgcg ggatcgagga gcaggacgcc aatattcacc cactcgtcgc ggatcagatt
     2401 cggctggtaa cgcaccacgt gataggcgca gggttgcagc gtttcgtcag gcatgtttca
     2461 ccttccaatt tggaaatggg tcgcggccgg aattgcgggc cgataaaagc aattcgcgca
     2521 cacggtcgcg gcggcggtaa aggcgctcga tcaactgctc catggcgttc cagtcacccc
     2581 cataccattc gggtggaatc tgcgcggact cttcgccgag cacgcgctcc gtgaattgat
     2641 tttcgaggcg ggacagccat ggctcgaagg cgcacattcc gtccacgccc tgatagacgc
     2701 gttgtcgcat gtacaggccg cgaagcggcg cgtcggggaa atcccactct cccgcgttga
     2761 agcaaaatcc gaaatcaatc atcatcgccg tgtaccccgc ggacgtgtac gtattaacca
     2821 tcgaggttgc agccgcatcg tccgggagaa aatttccggc cgctactgca agctcttcag
     2881 gatcgcgaaa aaaaattgcc tgccgtccat tagtgttgca cgtccactta tcgaggacca
     2941 ggacacctag aaaatcggaa agattccgaa cgcatcgtaa ttgctcgtcc ggcaaaaaat
     3001 cgtgcaccgt catgtgtgcc ggatgcccag ggaactgcga gccgaattgt tttccagccg
     3061 cacaaggctt tctgcccaag cccagctgca tgacgaggtc tggcgtatag gcgatgattt
     3121 ccgggcgcac ctccaccact tcgacctgag gcacgcacaa tcccagacgt gccgccagtc
     3181 ttgtagcaag catttcgttg gccagaattt ttaaatgttg cggattattt tgaaatttga
     3241 cgatgtaata agcatcgtcc tcgcagcgca tcagatgcgc ttgcgcgcca ccacgcattc
     3301 gccgcacatg ctcgatggca agtttcatga cagccatgct agacacgcgt ggcaaaaccc
     3361 gcaagaaaat agttccgcga tgggaaatga aaattacctg gagggaaatt tccgcgagct
     3421 gccccgcaaa ggtacatcac gcggcgaagc cctagaacgg acgaaggccc ggagtatgtt
     3481 cccgggcctt cgtcctctga atcgatcgct cgaaccgtgc tagcgctttt tgccgcgctt
     3541 cgccgctttg ttggccggct tctttttgga ggctttcttc gcagcctttt taggagcggg
     3601 ctttgccttc actttcgctt ttttctttgg tgccggtttc ttggctttct tcgccggcgc
     3661 ctttttcttg gcagccggtt tcggagttga agcgggttcg gaaaccgcgg ccatatccgg
     3721 acgttcggtt atgaccacct cttcttccgt ttcctcttcg actacatctt ccgggtcgcc
     3781 gccgagttct tcttcctcaa aatgcacgaa ctcgttttca tcgttttggt cttctttttc
     3841 gtaggccatc cgcattctcc cttcgactat tcgacatttc gaggaattcg atgacgattg
     3901 acgccatcgc gcgcttataa acggaagtca gcatacttgt caagagtcag gcatatttct
     3961 gctgcttccg cctgcaaaat ttcttcggca ttcacgccag gaggcaccgg acgatccggc
     4021 acaagcccgt catgaaatac cacaaatcca aacacaatat agagataaaa cggattaaaa
     4081 agcaaggaag gactagtgag tttgcagaat ggtcagcgta tcgccaacct gcaggggcct
     4141 gctgaacaaa taccggttgc ccgcctggat ggcttccacc gtggtctggt aggcgcgtgc
     4201 gatagaccaa agtgtttcgc cctgtttgac acggtgtacg accgagttgc ttgcccgtgc
     4261 ttccatgacg ggagacttcc gggttgaggc cgactgccgg gctgccacag gcgtcggaac
     4321 cgcggtcttg gtcttgttgc tcgcttcctg ctgttgggca gggcccggag tcatgccgcc
     4381 ggggtaaatc cgcaaggagg tgccgcgcac aacgcgatca gccttcaggt gattccactt
     4441 gcgaagttcc gccgcggtca cgtcaaactc gtcggcgatg gtggcgatcg tatcggtgcg
     4501 acgcaccctg tagcgaatca gtttcccgga agacgcctcg gttcgcgccg cagccggaat
     4561 aataagcttc tgcccttctt ccaattgcgt acccagggcc agttcattgg catccgcgac
     4621 ttccgcggta gaaacgcggt attgcttggc aatggaggac agtgtttctc cttgctccac
     4681 cttgtgacgg cgccagctga cccacttctc aggtggtatg gctgcaattt cggcgaagaa
     4741 atgttccgcg ctaccctctg gaatctgcaa aacgaaatcg ggatcgggcg gggtaaccag
     4801 ccgcaataat tccggattca tgctgcgcaa ggattccaga tcggtatcga tggtttccgc
     4861 taccagcctc aaatcaatgg cttggccggg ttttaccttg tcagccttca gggcaggctc
     4921 tggttcgaat tcgatgccgt atcgtgcagg atccttggag atcagggtga gtgccaggat
     4981 gatcggcacg tagttcatgg tctctttggg cagagcattc cggtgataca gttcccagaa
     5041 gtccgcatag ccagtccgct ccacagcatg ctggacggcg ccagctccgg agttgtacgc
     5101 ggccatggcc agataccagt cgccaaactg gtcatacaaa tcgcgtaaat cgcgcgccgc
     5161 tgctcgcgtg gccttttccg gatcctggcg ctcatccacc caccaggtct tttgcaatcc
     5221 gtattttcgt ccggcaaagg acatgaactg ccacatgccg cgcgcgcctg ccttggatac
     5281 ggcctgcggc aagaatgcgc tttcggcctg cgccagataa atcaaatcct gcggcatccc
     5341 ctcttctttc aggacacgac gcaccatttc gcggtagcgt cccgcgcggc gaagaccggt
     5401 ttcgacgatc gcgcttccgc gcggcgtctt gaaataattc aggtaagcca gaacaggatc
     5461 gttcaccgta agaggaagat cgtggtcaat ggctcccact tcaccttcgg cgcgtccacg
     5521 caggttggga tcgaattttt caggttgggg gatcggctgc tcggcaattt cgtcgattgg
     5581 cgcgggagtc gatttctgtt cgctgaaacc gtctccggcc cggaatgctt cgagttcgtc
     5641 caagctgacg gtgtcaataa tgtggtggta caaaccgctc aggcggggat cagaatcgag
     5701 atcgaagccg ctggcgagca agtggtccag tgcagcgtca aattcctcgc gcgccttgcc
     5761 cagatgtcca gccttaaaat cctgttcgcc ggcgtcaaat gccgtctggc tttcggtagc
     5821 cacttgctcg ggtccggagg gagccggagc caccacgggt ggcgcgattt tggtttgggt
     5881 ttctacgggc gcggcagtct gaacaggctg ttgcacgggc agggtaatct gcggtggctt
     5941 tacctgtacc agttttttgg aggagtcatc gcacccgacg aggcctgcga gaacaccggc
     6001 tgcgacaaat atccaagccg ccgatttttg agcgcgacat cccataaggt tttttttgaa
     6061 ccggtctgaa tcagtttgaa acgatgagtc gaaccgtcct ccgcatggaa gagagcacaa
     6121 tgctcgccaa acaaagaggc agattcccgc cctaggaatt ccgctaaact gattgtagat
     6181 cacatgcccc caagggggtc aatagggaat gctacagcct gttaatattt gtttaaccaa
     6241 ttgagaataa aaatcttagg agagggcgtg cttcaaatcc gcgatcagat cggaggcctc
     6301 ttccaggcca accgacatgc gcacagtacc cttaccgact ccggccgcag ccagctccgg
     6361 cgcggcgagt ccgaaatgag ttgtgtaaat aggtagcaca atgagtgatt caactccacc
     6421 caggctcgcc gcaagaagga aaatctcgga cttgtcgcag aatcggcgcg cggccggcaa
     6481 tcctcccttc atgtcaaaag ccaacattcc gccaaaacct ttcatttgct gttttgctaa
     6541 tcggtgatgc ggatgcgagg gcaggcccgg ataatgcacc cgctgaattt ttgggtggcg
     6601 ttccagaaat cgcgcaaccg ccatggcgct ttggcattgc cgccggacgc gaatctcaag
     6661 agtctttaat ccgcgtatca aaagataggc gggatctgga tccatggaac cgccgagaga
     6721 aatcatcatc tcgcgaactt ttccgacgcg ttcccgtctt ccggccacgg ctccggcaat
     6781 gatgtccgaa tggcccgcca gatattttgt tgcgctatgg accaccagat cgaatccgaa
     6841 gttcagcggt ttttgcagaa taggggtcgc aaacgtattg tcgatgatcg aaacgagttt
     6901 gtgttttcgg gccagggctg cggatctttt taaatcgacg aggccaagcg tgggattagt
     6961 cggcgtctcc acgtagaagg cgcgagtttt ggcgttcacc aaccgatcga agccgtcgag
     7021 gtttgcttcc acatggtgca cgcggattcc cattcggggc aggatgtccc gcatcagccg
     7081 gtaagagccg ccatagagct gccgtgtagc gatcagttca tctccggctt tcagcacgga
     7141 cagcaaggcg ctcgagatcg acgcggatcc cgaggcggtc acaagcgccg cttcgccatt
     7201 ttcaagtttg gcgattttct cttcggctac ggcgagcgtc ggattgccat agcgcgtgta
     7261 caagtatgct tttgactttc cctcggccca gcgtttcatt tccgccgtgt ccgcgaaagt
     7321 gaaattggcg gagcgaacga tgggtgcggt gacggaggcg ttcagcccat gcttcggctc
     7381 accgctgtgg atggcaagag ttgcgtcgga ccattgggga gaagttttct ttggcatagg
     7441 gccgttattt tttcacgcac ccaacaatac acaacctcca atttggcccg acgcgcaacc
     7501 gcgcgagttt ttctcttcct cccgttcctg tctccttgct tccttttgct tccttgccta
     7561 tgcgtctctt ttgctactcg cttcgagttt tcagacgtgc tcttcttcct cgacctcaac
     7621 tcctctagcc gccttcgccg cggcgatcac tttctccgcg atttccgccg gcacatagtc
     7681 atagtgcgaa tactccatgg tgtaggtggc ccggccttgc gtcattgacg tcaaggttgt
     7741 cgcgtaattc agcatttcgg caaagggcac tgacgctttc acgatgacgg tcgctccgct
     7801 cacttcgctg ccgttgatgc gcccgcgccg cgagctcaga tcgcccatga tgtcgcccga
     7861 atactggtcg ggcgcgaaga cttccacgtg catcacaggt tcaagaagcg tcgggcgagc
     7921 ttgcttcatg cattctttga atgcgaggga gccggcaatt ttgaatgcca tgtccgaact
     7981 gtcgacgtca tggtatttgc catcatggag gatagccttg aagtccacga cggggaagcc
     8041 agctaggtac ccgcgctcgg cggaagcgcg gatgcctttc tcgaccgagg gaatccagtt
     8101 cttgggaatc gcacctccaa aaatatcgtc gatgaattca atgcctgtcc cgcgcgccgc
     8161 cggctccaaa cgaacgcggc agacgccgaa ctgaccgtgc ccgccggttt gctttttgtg
     8221 tttgccctcg gcgtcagcct tgccgcgaat cgtttcgcga tagggcacct tgggaggatg
     8281 cagcttgagg tccacgtgat agcgtttgtg cagtttcgcg acgaccactt caatgtgctg
     8341 ctgtcccgag cccgccaata aaaattcctt ggtttgcgga tcgcgcgaaa atcgcagcga
     8401 tggatcttcc tccagaattt tgtgaatcgc gggaccgatg cggtcttcat ccgcgcgcgt
     8461 cttgggctca acggcgaagg tgatggaagg ttcagggaca tggggcagcg ggtaatggat
     8521 gggagcggac ttgtcgccaa gcgtgtcgcc ggtaaacgtt tccttcaatt ttgcaacggt
     8581 gcccaggtct ccggcatgca gctccggcac gccggttgcc gtctttcctt gcatcacctg
     8641 aatgtgctgc aaacgttcgc tagtgccgcg cgtgaaattt tgcggcatcg cgtcgttttt
     8701 caggacgccg gacatcactt tgaaataggt gacgcgccca gcaaaaggat cggcaagcgt
     8761 cttgaacaca aaaatggaca gtgcctcggc gtcggcgatt ttccgcttca tttcatttcc
     8821 tttgcttgcc gtttcgctgt ggccaatggc tgtaccgcgc tgcgacgggt ccgggaacac
     8881 gtcggccagt aaatcaagaa gcgtggcgct gccgatattg tgcagcgcgg acgtcagcac
     8941 cacgggataa atgcgtttgg cctggaatgc ctcgcgcagc ccttttctca aatcttcaat
     9001 cggtagcgtg cccttctcga aaaattcctc catcagcgcg tcgtcaccct cggccaccat
     9061 ttccacaagc gtttcgtgcg cggtcttcga cgcttcggcc agtaccgcgg gaatctcttc
     9121 gatcgtcggc ttgccgtcgc cgtccggcgt gtagagatga gccttcatcg tgatcaggtc
     9181 gactacgccg cgaaaccctc tctccgagcc gatcggtaac tgcagcggta ctacgttgcg
     9241 cccgaagacg cgcgtaatcg attccatcgc gcggtcgtga ttcgccaact ctcggtccat
     9301 ccaattgata cagaatgcgc gcggcacatc gtactccact ccgtagtccc aagctttttc
     9361 cgtgaccact tgcactcctg ccaccgcatc caccacgatc agcaaggcgt ccgccgccac
     9421 gagcgatgcg cgcgtatcgt ttaaaaaagt gctgtagccc ggagtatcca ggaaattaat
     9481 ttttattttt tcagagcggt cgctttttcc aggctggtca acctttccaa actgccccga
     9541 gggcgtccat tcggcgtagg ccaggctggt gtgaatcgtg atctttctgg cgatttcttc
     9601 gtcgtcccag tcggtgaccg tatttccttc gctaaccttt cccagccggg tggtcatccc
     9661 cgcggtaaac aataacgacg acaccagctg ggttttcccc gtgtcgccgt ggccggcaat
     9721 tcccacattg cgtatggatt gcgtttcgta ggctctcatc gctctacctc cttggcgccg
     9781 ctgttgggga gcggcggttg ccgcggccgc atcggaaaaa gaattgcggc gtactgctgg
     9841 gaaggatgaa ggttcgtgtt ccgggtttcc ggtcgcgccc tcctattttg cggatgctag
     9901 cacgatagga gatgcgcggc aatcgggtca gtggattttc actgggccac tttttttgtg
     9961 caacctctcc gtcccggatt catctgaccc caaaagggtt aattgcaggt ggagcccggg
    10021 tgccatggct gtccctttcg tggctgaata cccgttcgat cgatgatcga ttcaattcgg
    10081 gaggtttttc atgttgctcg cgctcgaagt tgtaactgta tttcttgcag caatcgccat
    10141 gagcatggcg ctcgcgcatg cccttgaata tcccggcaag aggcggctcg atgcggagac
    10201 gtacatggcg gtgcagacga tttactaccc gggattcacc ttgggaggaa ttggcgaacc
    10261 actggctgca atatccacgc tcctgctttt gattgccgtg cgcgatcgcg gtgtggtttt
    10321 ttggttgata cttggagcgc tcttcgcgct ggttgcgatg cacctggtat tttggtttgt
    10381 cacccagccg accaataaat tttggctcag gaatcagcaa ctgagcgggg caggagccag
    10441 attcttcaat gtcgatcgaa gcgaagcagg ctcaactgaa gttgataggg ccaacccggc
    10501 aatgctgcgt gaaaaagtga atccggaatg gaagcgtttt cgagaccggt gggagtattc
    10561 acatgttatc cgtgcagctc tgtctgctat ggcattgatc gcactcatta tcgcagtggc
    10621 catgtgagaa gttttgtgct tgcaaggacc agaatccacg gactgaagag tctgtgtgag
    10681 aactgcaaaa ttggaacttc agcggctaaa gccgtactca ttttgcagaa cttatgtcgt
    10741 ggctaaagcc acgacccaca aagattctgc agttctcacg cagactctga agcctacgcc
    10801 acttgaaagc gattactgcg gaaaattccc ggtgaacacg acttgcggtt tgcgcggatc
    10861 gcagcggcga atctggccga tgcggaaata tttttcgcgg cgtcgcttca gttcggtttc
    10921 gagggtgcga tgattttcga tgggcacgac aaggatcatg ccgacgccca tattgaacgt
    10981 gcggagaaga tcgtcgcgct cgagtttgcc caggccggcg aggtacgtaa aaatcgacgg
    11041 cacgggccac gaaccaagtt cgatttccgc ctgcacgccg cgcggcaaaa cgcgcggtaa
    11101 atttcctggg atacctccgc ccgtgatgtg cgcgatggcg gagggccagc ctttggcgac
    11161 cgtggttttg agcatgggcc agtagcagcg atgcggccgc agcaattcgg cgccgatctt
    11221 gttgttgact tcggcgacgt aggattccgg atgcaagcct gcgtggtcga atattaattt
    11281 gcgcgccagc gaataaccgt tcgtgtgcag cccggtggag ggaagcgcga aaagaaggtc
    11341 gcccggtttc acgacatttg tttttttcag gctggcacgc tcggttacgc cgacgatgaa
    11401 ccccgcaagg tcatattctc ccggcgcata aaaacccggc atttcagctg tttcgccgcc
    11461 gatcagcgcg catcccgccg tgtggcaagc gcgcgacatt ccctccacca gctgctcgac
    11521 cacgttcgga tccatttttg acatcgcgag gtaatcgagg aagaagagag gttcggcgcc
    11581 aagcgtgagg atatcattga cgcaatgatt cacgagatca gcgcccaccg tggagtggat
    11641 gttcatggcc atggcgattt ttagtttggt gcccacgcca tccgcgctcg agaccagcac
    11701 cggctctttc cattttttat ccagcgcaaa caatccgccg aacgatccga taccgccgat
    11761 tactccgcgc gtaaacgtgc gcgacgccag gcgccggatg cgctccttgg cctgatccgc
    11821 aagggaaata tccacacctg cttcggtgta gtccatgtac gttgaactct ttctgccgtg
    11881 gcgagaggag ccgggcggcg tgctcccgta agattaaagc atttggccga ttaaaataat
    11941 tttccgatta aaacaatttg aagatacgtc gccagcgcac aatactcgga tgcagcagaa
    12001 tgcttgcgta ggcggtcatg cgatcccgca cgtgccccgg attccaggta tcctcgggcg
    12061 cgtcgatgga catatacacc atgatgggag tcccgataat gttttctctg ggcacaaatc
    12121 cccagtacct gctgtcgtag gaatcgtctc gattgtcacc catcatgaaa tattcgccgg
    12181 ccggaaccgt tacagggccg tagttgtccg tcgggttcgg cgggtccgcg cggtagggct
    12241 ctttcagtgg cttgccattg acggatacga cgccttcgcg aatttccagt tgatcgcccg
    12301 gcaatccgat caggcgcttg atgtaatcgg gggttccggg cagcggcgcg cggaaaacaa
    12361 ttatttgctg gcgacgcgga tcgcgccaaa ggcgcatgtg cagcggcgtc atcggcaatt
    12421 cggcttcgta gccgaaacgg ctcatcagta gatgatcgcc gacgagcaag gtcctttcca
    12481 tggaaccgga aggaatgacg cgcgcctgca cgatcgttcc ggtgatgaac aaaaatgcca
    12541 aaataaccca aagccaggac agcgcttcgc ggagaataat tttatttata tccggtttgg
    12601 gttgtactac aactggaatc gtttcttcag cgttcattag gcatcacctg aatttctacg
    12661 gccggtacat cgatttccat tgttcgtgat tcgagctcgg cttgcgctcc gtctaccggg
    12721 tacacgcccg tgtagcagga ggtgcagaaa gagccgcgtg tatcatccac cgcgaccttc
    12781 aaatttgaaa gcgaaacgta acccagcgta tccgcgccca gatattggcg aatttcttcg
    12841 atggagcgct gctcgcgatc gggtgggagc gagccctggt caagcttcgc ttcaaaggca
    12901 gtaagttctt ccgcggtaag ccgagggcgc acgcgcgatt catcggacgc aattaattct
    12961 tcgcgcgtcg gcgtatccac gccgtaatag cacggcgaaa tcgtcggagg gcagctgatg
    13021 cgcatgtgaa cttcagttgc gcccgcttcg cgcaccatgc gcacgatttt gcggctggtc
    13081 gtgccgcgca caatggaatc gtccaccagc accacgcgtt ttccacgcag aagttccggc
    13141 acaggattca gtttcagctt cacgccaaag tcgcgaatgg attgctgcgg ctcgataaac
    13201 gtgcggccaa cataatgatt gcgaatcagc cccatgcgga aagggatgcc ggattcctgt
    13261 gaatacccaa ccgcggatgg aacgccggaa tcagggacgg gcacaacgat gtcggccgga
    13321 acatggtgct cgcgcgcgag catgcggccc aacatttcgc ggctctgctc gacgacgcgg
    13381 ccgaagacca ggctgtccgg gcgcgcgaag taaacatgtt caaaaatgca ttgctgcaag
    13441 ggtttcggcg cggcgaaacg cagggactca atcccagcgc gcgagatgcg caacatttct
    13501 ccaggctcaa tgtcgcgcac atacgtggcg ccgattaaat caaacgcgca ggtttccgag
    13561 gccacgaccc atgcgccatc gagttgcccg agcgccagcg ggcggaagcc gcgcggatcg
    13621 cgcagcgcgt acatttcgtc acgcgtgagc acgagcaggg aatacgcgcc ttcgacttga
    13681 ttcagcgcgt cggcgagggc gcttgaaaga ttgcgcgcct ggctgcgcgc gatgaggtgc
    13741 acgatgactt ccgtatcgct tgtggtttga aaaatcgagc cgcgatgttc gagcgtgcgg
    13801 cgcaatcgcg cggcgctcgg cagatttcca ttgtgcccga gggcgagttt gcccttgttg
    13861 cagtcgatca cgatcggctg cgcattcatc agggaagtat cgccagccgt ggaataccgc
    13921 gtgtggccca gcgccgcttc gccaggcagg cgcgcaagca catgaggctg aaaaatatcg
    13981 gcgaccaggc ccatactttt ttccaggtat agttcgcgtc catcgctcga aacaattccg
    14041 gcggattcct gtccgcgatg ttgcaatgcg tagagtccga gataagcgag tttggaagct
    14101 tcgggatggc cgaaaacgcc gcacactccg cagtgatcgt ggaaccggtc attctcaatt
    14161 tcgatcaaac gattcattct gccttccgga gatcgttggc attcaggagc cgtccgaggg
    14221 ctccgctcca tgcgtcagcg agggagggga cttcggcgct gactatcatc tgtccattca
    14281 gttcgatgcg gaattcgtct gaagcgactc ggccaacttc cacggctgcc acctcatatt
    14341 gtgccgcaat tcggcgcacc gcggcaacat tttcaggagt aactgatacg actgcgcgcg
    14401 cgccgcgttc gccgaagaac gccaactcgt ccggctcttt gcttgtaagt gaaatttgtg
    14461 ccgaaagtcc attgctcgtg aaacacgatt cggcaagcgt tacggcaaga ccgccgtcgc
    14521 ttacgtcgtg cgcggattgc aactggcctt cttgcgccag tgcaaccagc agggcaatca
    14581 gccgtttttc tgccgggaga tttatggcgg gaggcgcgcc ggcttcgacg ccctgtgtgg
    14641 ttcgcgcgta ttccgaggag gaaaattcct tcgcggcatt ttcgtccgag cttgatgcac
    14701 tcgcaattcc gtcgagcagt aagattgcat cgcccgtggc gcggaaggcc atgcccagcg
    14761 ctcgcggcgc attctcaatc attccgagta cgccgagaac gggcgtcgga tcaatggagc
    14821 ggccaagtgt ctcgttataa aaacttacgt tgccgccggt gatgggaatt tcaagcgcga
    14881 tgcatgcctc ggagatgccg tcaatcgcgc ggctgaattg ccacatcact tccggctttt
    14941 cggggctgcc aaaattcagg caattcgttg cggccatcgg cttcgcgccg gagcatgcca
    15001 cgttccgtgc ggcttccgcc acggcgagtt gcgcgccgac aaaaggatcg agacggcacc
    15061 atcggccatt cccatcggat gcgagcgcga gcgcgcgttt cgttcctttg atccgtacga
    15121 ccgcggcgtc gcctgctccc ggtgcttcga tcgtgttggt gcgcaccatg taatcgtact
    15181 gttcccagat gtagcgcttc gacgcgatcg cgggagatgc gagcagagcg cgaaaatttt
    15241 ccgtgagatt tttgccaggc tcggcgaacg caaccaaatg ctcgttcgtt accggcgccg
    15301 gcgcagcgag tggacgctgg taaaccggcc cttcttcggc aagcgcgtgc gcgggaattt
    15361 cggccaccac ttttgcgtga tgcagaactc gtacgaagcc atcttcggtg acgcgtccaa
    15421 tttctaccgc gtccaagccc cacttcgcga atacatttaa aacttcctgc tcgcggccgc
    15481 gcgcggcgac aagaagcatg cgctcctgcg attcgctgag catcatttcg tagggcgtca
    15541 tgcccggttc gcgctgcggc acccgcgtga gttccatttc aatgccgagc ccgccgcgcg
    15601 atgccatttc gcaggaagtg ctggttaagc ccgccgctcc catgtcctgg atggctacga
    15661 ttgcgccggt cttcatggct tcgagacatg cttcgagcaa aagtttttcc atgaacgggt
    15721 cgcccacctg cacattgggg cgcttttgtt gcgactcctc tgtgaattcg gcggatgcga
    15781 gaagcgacgc cccgtgaatg ccgtcgcgac cggttttcgc gcctacatag ataactggat
    15841 tgccaactcc gcgcgctttg gcgaaaaata aatcttcctt cttcgcaatg cccagcgcca
    15901 ggacgttgac caggggattc gcggaatacc ctgcctcaaa ttgcacttcg ccgccgacgg
    15961 ttggaacgcc gaaacaattt ccataatgag agatgccgcg cacgaccccg tctaggatgc
    16021 gccgattgtg agcgatggtt tcagcgtcga tgcgatccgc gggttgaggg gctaaagctc
    16081 cctttgtagt cacttgtccg gacgggctga agcccgtctc tccagcccct gaaacattcg
    16141 cagaaatggg cccaaagcgc agcgagtcaa ggactgcaat gggtcgcgcg cccatcgtaa
    16201 aaatgtcgcg cagaattccg ccaacgcccg tcgccgctcc ttgaaacggt tcaacgtagc
    16261 tcgggtgatt gtgcgattcc attttgaacg cagcggcgag gccgtcgccg atgtccacga
    16321 cgcccgcatt ttcacccggt ccctgcaaca cttgcggtcc gcgcgttggt aatcggcgca
    16381 aatgcacttt gctcgatttg taggagcagt gttcgctcca catgacgctg aaaatgccaa
    16441 gttccgtaat attcgggtcg cgacccagaa ttttctgaat gcgcccatat tcgtcggcag
    16501 tgagaccgtg ctctgccgcg agttgcggcg tgactttaat ttcggtgtct agcattggtt
    16561 tagacgctga caaatttctg gaaactccgg tagcacaggc ttcagcctgt ggatttaaat
    16621 tacagaatga tgactttgcc cggtcaaatt cagggactga gagccacagg ctgaagcctg
    16681 tgctgttaac ttctaagccg aaactcctgc ttgaattaaa agcgattcaa ttagagaacg
    16741 aaaaatgacc tggccatcgg tcgagccaag cgcggactcc accgcgcgct cgggatgtgg
    16801 catcaggccc acgacatttc gttctcgatt gcagatgcca gcgatgttcg cgagcgaacc
    16861 gtttggattg gccgactcat cgatgcgccc atcaggcgtc gagtatcgaa aaataatctg
    16921 ttcgttgcgc tggagttcgc tgagtgttgc ttcgtcgcaa aaataatttc cgtcgccgtg
    16981 cgcgatggga atttgcagca cctggcccgg atcggctgcg ccggtaaaag gcgtgtccgt
    17041 ggattcgact cggatatgca cctggcggca gatgaacttc attcccgcgt ttcgccgcag
    17101 cacgcctgga agcaatcccg cttcgcacaa aatctggaag ccgttgcaga taccgatgac
    17161 aaggccgccg ccgtgagcaa acttttccac agcgcgcatc acgggagaaa atcgcgcgat
    17221 cgctcccgtg cgcagataat cgccatagga aaatcctccg ggcagcagaa tggcgtcaaa
    17281 attatttact tcttcggatt gatgccacag gaattcaacg ggctggtgga gaacttcgcc
    17341 gagcgcgtac caagcgtcgt gatcgcaatt ggagcccggg aacactacta ctccaaattt
    17401 catcgcaaaa aatcccccgc gatattttaa cacatgcagc ggggatattc tgtctgcgga
    17461 atggacgcgg cgtcaaaatc gacttcaaat aaatttaaat agtccgattg atataacttg
    17521 gaggtcgtta tggctagcgc ctattcggtc ttgtcttttg ctttttgagc accttcctcg
    17581 gttttatccg ctgccttgtt ggttcccttc ttaacagcgt ggccggtctt cttggcagtc
    17641 tttttcgtgg cccttccggt tgccttggcg gcatcttttg tatcctggcc cgcttccttc
    17701 gtgtcctgct ttgcagaatc ctttgtagag tcctttgtag aatcctgcgc catcaggtgc
    17761 accggtcccg tcgctaatag cagcaacatc gccaaaggcg cgaaaacgat tttcgatatt
    17821 cgaatcataa aaacccccgc agtttctctt gcaaacaaaa tggtggcagg gaagaaatcg
    17881 gagtgaaata cctattactc cctacgggcg ggaatagaag ttagtacgta aacaactgcg
    17941 gtgtcatgga gtatgactcg tgcggggtag gataaaattc caaaatcgct cggatacact
    18001 cgcggagggt ctgattttga aaggcttatc gcggcaattc gaagcctcct ggcccttgcg
    18061 gaacggcttt aatgtcccga aacgtgtttc ggaagcatct gaccagggcg acgatgaatc
    18121 gcaaggttta ccaactccag gacgactgga gttgccagac ctactgcaac agagtcaacg
    18181 gccaaaccga gaatgccgcc tgccagcagg aacatgccaa gcacagcgag attcacgctc
    18241 tcgtaaactt ccagaacgag atcaccaaaa atgaaagcga ttgcagcgac tacgaggccc
    18301 cagttcaccc aaacgcccat gaaacggtcc tcccaacagc ggatttaagg cgacaatatc
    18361 actaggaggg gaaagttaca acaaattcga tagggtgccc cgtacccgga aggtaattag
    18421 acgcgagacc agtaattatt gagaatggcg agatgaaatt gttggagcta cgcaggacgt
    18481 ggcgatatta agttgcacag acttcggccc gtcgattttg atttcttttg ttcatgcaat
    18541 tttagttgta tgcagaaaag ccacaggctg aagcctgtgc tacttagatt tcctttttgc
    18601 ttttttgcca gtgcccaatt ttttagattt agcggccgcc atttctttgc ggcgcgacgc
    18661 agcccagcca agtttgttca tctgttttcc gcgagcaagg cctagtgcat cggccggaac
    18721 atctttcgta atcacggaac ctgctccgac gtacgcgccg tcgcccacgc gcacgggagc
    18781 gacgagcgcg gtatcgctgc cgatgaaaac gcctttgccg atcgtcgtag gattcttgtt
    18841 tacgccgtca tagttgcacg taatggttcc cgcgccgatg tttgttttgc tgccgattcg
    18901 cgcatcgcca agataggtga ggtgcatgga cttgacgccc tcgccgagca cgctcttctt
    18961 tacttccacg aaattcccca cgcgcgcgtt ttcttccaga cgcgcgcttt ctcgaatgtg
    19021 cgcgaacgga cccacttgag ttccacgtga aaggtggctc gctgtgacca tcgtataagg
    19081 tttcacgagc acgttatcgg caagcacggc gtcggacagt acgctcccgg tacgaatcgt
    19141 gcatcccgcg cccacgcgag ttttccctaa aagttgcacg ccgggttcaa tacgcgtgtc
    19201 ggcgcccacc atgacctcgg gatcgatcag cacggtgtca ggcaattcaa tcgtcacgcc
    19261 cgcgtccatg atggctgcgc gtttgcgctt gcgaaaaacg gagtcgactt cagccagatc
    19321 ggcgcgcgtg ttgcagccca gtacttcatc cgggtctgat gccagtacag cctgtacttt
    19381 ttcgcctttc tcgcgcagta cggcaatggc gtcagtcaga taaagctctt tgtgcacatt
    19441 tttcggcttc agtcttgaga ggcatggcca caatttcgca agcgtgaagc agtatatgct
    19501 ggaattgatt tcgcgaatga gccgctgctc gggagtcagc gcggaatctt cgacaatggc
    19561 ggaaaccgaa ccgtcgtcct ggcgtacgat gcgtccataa ccggagggat tttctatttc
    19621 ggcggagaga atggtggctg cggcgccgct ctcgcgatgc gagcgcgcta gcgcggcaat
    19681 cgtcacggtg cggatcaagg gcgcatcgcc cggcaaaata attacaaatt ttgcgcgcgt
    19741 cggaatggcc cgccgcgtga ccagcatcgc gtggcctgtg ccgcactgcg gctcttgcaa
    19801 aatagttttc acgttgagcg gagcgacagc ggcggttaca tcctccgctt ggtaccccac
    19861 gaccgccaca atttcacgcg gcttgatggg ttggcaagcg cgcactacgt gctcgacaag
    19921 cggcctgccg cccgcgcgat gcaaaacttt tgctagcgcg gattttagac gcgtgccctt
    19981 acccgcagag agaaccacaa ttacaaaatc ggaattagcc atggctgtat tttcagcgtt
    20041 cctcgataga atgacaatcc atggtaatca cgagggatgc gaaacgaaca ctttatagaa
    20101 ccaacacaat agggaggtgt cgacgagatt tggaaaggaa agctcagtcc ccagcagtag
    20161 caggtgcttc ggcaaagtgt tgcggcgtct ccgcaggagc ttcgtcatag gtctcagtcc
    20221 acagggtcgg gtccgcaagc aggcgcgtaa ctaactgcgt atgcagcgcg tggcccgctt
    20281 tgtaggcttc gacgcgtgcc agcagcggcc ggccaaccag cgccaggtcg ccaatcagat
    20341 ccagcgcttt gtgccggccg aattcgtccg ggaagcgcag agggccgttc atgactccgt
    20401 cttcgttcaa tacaatggca ttttcgagag agccgccgcg gatcaaaccc attcgccgca
    20461 gtccttcgaa atcttgcacg aagccaaatg ttctcgccgg cgcgaggacc cggctaaaag
    20521 attcacggct cactcgcagc tcaatttcct gggcgcccac tgctttgtgg gcatattcca
    20581 cgtagcatcg aacgctgaaa tcatccgccg ggtatattcc gatgcgccgt ccagcgtccg
    20641 aaaattccag tggtttcaag atcttcaggt atcgccggcg cttgcggagc tttcggattc
    20701 cggcttggcc cagcatttca ataaatggtt gcgacgagcc gtcgaggatg gggagttcca
    20761 aggcatcgat ttcgatgtag acgttatcga cgtcgcaaga atacagagcg gcaagtagat
    20821 gctcggtggt ggagagcaac acgccttttt tcatcaggct cgtggcatag ctgacgcgcg
    20881 ccacgtttcg cacgtgcgcc tcgatctcaa aattatcgag atcgactcga cggaagacga
    20941 tgcccgtatt ggccggggcg ggcacgaggc gcagtttaga ctttacagcc gtatgcaggc
    21001 ctattccgct tgtctcaacg ggcctttcga ttgtggtttg gaagctcata tccgctgaat
    21061 acgctagcat gttgttggcc ttatattaaa tcgtttacac gtttagaatc ataagcttag
    21121 aatggatatt ggaattgcgt gtggtaaaag agacacaaag cacatcgttg cgcgtggtgg
    21181 aatagcatca aaaccacgtg atcagagacg gctgtaatcc tcaaattttt aagagcggtg
    21241 cctttcacgg ataagttaaa tagacgagtc agtgacggca cgggggcgct tgtgtctgtt
    21301 ccgaggtccg catagaatga atggctgaaa aaggctcaga tggcttcccc atttaatatc
    21361 atttcaaatt cggttcccgc gcaggctccc ggagcgtcga tcgatccgtc ggcgcacccc
    21421 aggccggatt ggctgcgagt caaatttttt aatggcccaa aatatcagga tttgaag
//
LOCUS       DASZMB010000016         3780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1112485, whole genome shotgun sequence.
ACCESSION   DASZMB010000016 DASZMB010000000
VERSION     DASZMB010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112485"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="VG272_00620"
     CDS             <1..1082
                     /locus_tag="VG272_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12211.1"
                     /translation="TPYTYQYNLSLQRELTPNLSVEVGYLGSSSHGLTSLQDVNPFVL
                     GTTDRVLNLGAGDTTCPDQTAGNPNLFGCSFAEISEFKNVANANYNALDLTLREQVNR
                     SRKFGNVYFTFGYTYSHGIDDASGFNQRNRNVPSYSSALLRASSDMDVRNRITFSGAW
                     DLPFDNMWREGPKRLTQGWSLFPILTWRSGFPLDIFAELPDRFNAGSEGPSGAGDPQV
                     VHANIVGPTNTMDPKANGGLWFNPASFSNARCSDPGGPACTPGPDMFPADYQVVANPA
                     LATYGSLPRNSLRGPGQFYINLSISKSTAITEHTGLKFEVDIFNVFNRAEFLNPDTNI
                     TDPTFGQITTTYDPRIIQLAARFSF"
     gene            1364..>3780
                     /locus_tag="VG272_00625"
     CDS             1364..>3780
                     /locus_tag="VG272_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWF12212.1"
                     /translation="MATCVKCKTPFQFDGQTRYETISGGMLDQETTPQGMSAAWSMAV
                     TPPAGAAAGQGGELVGTILADRYEIISLLGQGGMGAVYKARDNELERTVALKLIRPDL
                     ASNPEILRRFKQELILARDVTHRNVIRIFDLGQANGIKFITMEYVEGRDLRALLHEKG
                     KVPVEEAVEIIAQICRALESAHIAGVVHRDLKPQNIMLDAKDRVYVMDFGIAHSLETP
                     GMTQTGALMGTPEYMSPEQAKGAKIDARSDLFAVGIIFYELLTGISPYKADSAIATLL
                     KRTLERPLSPTEVDAAIPQSISDVVMNCLAIDREQRYASAREILEDLGQEGPTAGRTM
                     ARTLAPSFPTVVPAPPPANVPFWVRKQIWIGAAAISIFILAAAGLFLRGKLFSASSGS
                     SGPPVEQASLAILPFRNGSGDASLDWLGPSLADMVSTDVGQSASLRTISADRLHQVLT
                     DLRISAGTTIDPAMVGRIAEFSNADTVVSGQYAKFGEQIRIDATLTDVKRNRTTSLTI
                     NAASEKEIPGTVDGLAELIRKNLAVSSDVLKELKASSFQPSSKSVPALRDYDQGVQLL
                     RDGKNLEAVKMFQTAIQEDPQFALAYSHLAETDSELGYDDDADKYSRQAIDLSRELPL
                     AERYQIEGNRARIMKDTKKGIEAYENLAKTFPNNADVEYALGSLYEDDGNFEKALGHY
                     GNVLKSDPKNLDALLATGRVESKSGDPQAGLDSLGRALSIAVQLQNPEQQAMILQATG
                     ISYKQLGRSDDALNNYKQSMDINTRIGKKRGVAANLSEIAQVQARMGNSDQALANLTK
                     ALSLQRE"
BASE COUNT          908 a         1019 c         1024 g          829 t
ORIGIN      
        1 ggactcccta cacgtatcaa tacaatttga gtctgcagcg cgaactgaca ccgaacttat
       61 ccgtggaggt gggatacctc ggaagctcct cgcacggatt gacttcgttg caggacgtca
      121 atccattcgt attgggaacc acggatcgag ttttgaatct tggcgcgggg gatacaacat
      181 gcccggatca gactgccgga aatccaaacc tttttgggtg tagcttcgcg gaaatttcgg
      241 aattcaaaaa cgtcgcgaat gcaaattaca atgcgctcga tctgaccttg cgggaacagg
      301 tcaacaggtc gcggaaattc ggtaacgtgt actttacctt cggctatacc tacagccacg
      361 gtatcgacga tgcgtccgga ttcaaccaac ggaacaggaa tgtcccttcc tacagttctg
      421 cattattgcg agcgtcgagt gatatggatg ttcgcaaccg aattacattc agcggtgcat
      481 gggatttgcc tttcgacaac atgtggcgtg aaggccccaa gcgactgacc caaggctgga
      541 gcctgtttcc catcctcacc tggagatccg gttttccgtt ggatatcttc gctgaacttc
      601 cggatcgatt taatgccgga tcagagggac cctccggcgc aggtgaccct caagtcgttc
      661 acgcaaatat tgttggaccg acaaatacga tggatccgaa agcaaacgga ggattgtggt
      721 ttaacccggc cagcttcagc aatgcgcgct gctccgatcc tggaggtccg gcttgcacgc
      781 ccggtccgga catgttcccg gccgattatc aggtcgtggc caatccggct ctggcaacct
      841 acggctcact gccgcgcaac tcgttgcggg gaccgggcca gttctacata aatctttcca
      901 tatcaaagtc gacggcaatc acggaacaca ccgggttgaa attcgaagta gacattttca
      961 acgttttcaa ccgagctgag tttctaaatc ccgatacgaa cattaccgat ccgacgttcg
     1021 gccaaattac caccacctat gacccgagaa tcatccagct cgcggcgcgg ttttctttct
     1081 agccagatga atttcgtatt tcggttttca cttcggttga tttggttcga tgttcgatgt
     1141 aagttgcggt gagcaggcgg cacgtcaaaa atgaatcggc tttgtagacc atgacagtgg
     1201 attcgccggg actgctacat ctttcaaagc agccttggca agatccttac tcagcaattg
     1261 ccgcttgtat ttccgcaagt aaatcccata attagggtgc tttggagttt taaactccca
     1321 gtctatgcaa tgtcctcaat gcaaatcgga aaatccgccc actgtggcga cgtgtgtaaa
     1381 atgtaagact cccttccagt ttgacggcca aactcgatac gaaacaattt caggcggaat
     1441 gctggatcag gagacaacgc cgcagggaat gtctgccgcg tggtccatgg ctgttacgcc
     1501 cccggccggc gccgccgcgg gacagggcgg cgaactggtt ggaacgattt tggcggatcg
     1561 ctacgaaatt atttcgctgc tcggtcaagg tggaatgggc gccgtgtaca aggcgcgcga
     1621 taacgagctg gagcgcacgg ttgcgctcaa actgattcgt cctgatcttg ccagcaaccc
     1681 ggagattttg cggcgattta aacaggaatt gattctggcc cgcgacgtca cccaccgaaa
     1741 cgtgattcgc attttcgatc tggggcaggc taacggaata aaatttatta cgatggaata
     1801 tgtagagggc cgcgatctgc gcgccctact gcatgaaaaa ggaaaagtcc cggtcgagga
     1861 agccgtcgag attatcgcgc aaatttgccg cgcgctggaa tccgctcaca tcgcgggcgt
     1921 cgtgcaccgc gatttaaagc ctcaaaacat catgctggat gcgaaggatc gcgtttacgt
     1981 gatggacttc ggcatcgcgc attcgctgga aactccgggc atgacgcaaa ccggcgcgct
     2041 tatgggcacg cccgaatata tgtcgcccga gcaagccaag ggcgcaaaga tcgatgcgcg
     2101 gtcggatctt tttgccgtgg gtatcatttt ctacgaattg ctgactggaa tttctccgta
     2161 caaagcggac tccgcaatcg ccacgctgct gaagcgcacg ctggagcggc ctctatctcc
     2221 caccgaagtt gacgctgcta tcccgcagag catcagtgac gtggtgatga attgcctggc
     2281 cattgatcgc gagcagcgct atgccagcgc cagagaaatt ctggaagacc tcgggcagga
     2341 aggtcctacc gcgggccgca caatggcgcg aacgctagcg ccgtcgttcc caaccgttgt
     2401 cccggctcct ccacctgcga acgttccgtt ctgggttcga aagcaaattt ggattggcgc
     2461 ggccgccatt agtatcttta tccttgcggc tgccgggctc tttttgcggg gaaaactttt
     2521 ttccgcatca tcgggctcga gcggccctcc ggtggagcaa gcgtcgctgg caattcttcc
     2581 tttccggaac gggtctggcg acgcatcgct tgactggctg gggccgagct tggccgacat
     2641 ggtcagcacc gacgtcgggc aatccgcgag cttgcgcacg atttctgcgg atcgcctgca
     2701 tcaggtgctc accgatttac gcatcagcgc cggtacaacg attgatccgg ccatggtcgg
     2761 ccgcatcgcg gaattcagca acgctgacac tgtggtttcc gggcaatatg caaaattcgg
     2821 cgaacagatt cgcatcgatg ccacgctgac ggatgtgaag cgcaatcgaa ctacgtccct
     2881 gacgatcaat gcagccagtg aaaaagaaat tcccggcacg gttgatggcc ttgcggaact
     2941 tattcgtaag aatcttgcag tgtcgtccga tgtgctgaaa gaattgaaag cgagttcgtt
     3001 tcaacctagt tcaaaatccg ttccggcgct tcgtgattac gatcagggag tgcaactcct
     3061 gcgggacgga aaaaatctgg aagcagtaaa aatgtttcag accgcaattc aagaagaccc
     3121 gcaatttgca ctggcgtact ctcatctggc ggaaacagat tcggagcttg gctacgacga
     3181 cgacgctgat aaatattccc ggcaggccat cgacttgagc cgggaacttc ctctcgccga
     3241 gcgataccag atcgaaggca atcgagcgcg catcatgaag gacacgaaaa aaggaattga
     3301 agcgtatgaa aatctcgcaa aaactttccc caataacgca gatgtagaat acgcgctcgg
     3361 cagcctgtat gaggatgacg gcaactttga aaaagcactc ggccactacg gcaacgtttt
     3421 aaaatcggat cccaaaaatc tggacgcgct gctcgcgacc ggtcgcgtcg aaagcaagag
     3481 cggtgatccg caggccggac ttgattctct cgggcgcgcg ctgagcatcg ccgttcaact
     3541 gcaaaatcct gaacaacagg ccatgattct gcaagccaca ggcatctcgt acaagcagtt
     3601 gggccggtca gacgacgcgc tgaacaatta caagcaatcg atggacatca acacgcgcat
     3661 cggcaaaaag cgcggcgttg cggcgaacct gagcgaaatt gcgcaagtcc aggcgcgcat
     3721 gggaaattcg gatcaggcgt tggcaaacct cacgaaagcg ctgagcctgc aacgagaaat
//
LOCUS       DASZMB010000017         5787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3315482, whole genome shotgun sequence.
ACCESSION   DASZMB010000017 DASZMB010000000
VERSION     DASZMB010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5787
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3315482"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VG272_00630"
     CDS             <1..304
                     /locus_tag="VG272_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12213.1"
                     /translation="MMHRPSVLILDEPTRSLDPLAAADFRKFLVREIVGAHGTTMIFA
                     SHTLTEVEQLADKIAMLDDGRLVAWDTLDRIKSATGVSSLEESLEILTRHVVRKKP"
     gene            301..1134
                     /locus_tag="VG272_00635"
     CDS             301..1134
                     /locus_tag="VG272_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF12214.1"
                     /translation="MNDSLKLGMTLRKLMLIFRRDFTVARSYRFAFVINLFQALFGTA
                     SFYFLSRFIESPTLQKSLPAGTNYFSFVLVGIAFFDYLSVALSTFDSSLQEARQNGTL
                     ENLLVTQTSLPIILAGSSLYPFAGMSLHTVIYLGWGALLFGFPLHGANWLGAVLVLAA
                     SILAFSGFGILSAAYLLVFKRGNPVNWAILGLSSVVGGMMYPVSVLPKWLQYVARLTP
                     VTYSLEGMRAAILGHASLRELLPSIAGLFLFAAILLPISFAIFSWALRRAKITGTLTH
                     F"
     gene            1233..1529
                     /locus_tag="VG272_00640"
     CDS             1233..1529
                     /locus_tag="VG272_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HWF12215.1"
                     /translation="MPKAAQGTSIVVSALQARTNFGRLLRQIEEKPCSLVIEKRGTPR
                     AILLSIRNYVRLAAPEPEVLKAIGKQSEGKGTDALSSRQIDQIIKAARAKKAKR"
     gene            1529..1951
                     /locus_tag="VG272_00645"
     CDS             1529..1951
                     /locus_tag="VG272_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HWF12216.1"
                     /translation="MLPLRLVLDTNIIVSAAIKPEGLQRTVLLLAMTRPARLYVSEAL
                     LSEYRMVLSRPEFKIRKGLRLQFLQRIKDASRLASPSRQIRVTPDPDDNIFLECADAA
                     RADYLVTGNLRHFPKFWKQTKIVSSREFITMITPHLVP"
     gene            complement(2125..3300)
                     /locus_tag="VG272_00650"
     CDS             complement(2125..3300)
                     /locus_tag="VG272_00650"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF12217.1"
                     /translation="MNRMKVIRKAIMLALIAGPLAVLLAPAAFAQNATLSGTVLDLTG
                     KPYPNVTLTMKSVETNKETKVTTDDKGHYSAPGLAGGTYNVDVTGKDKDQKDELLYQT
                     GLKLAPNTSPTFDINFKELQEQGKLAAVEAERKRQEAEAQFAALKTHYDAGIAAIEQM
                     KAAQTKLAQTPKEQQDPIKAQVTQAGGTAVTELSAALELEKPEDPNRSIILSRLGEAY
                     ESMGKWQDAADTYQKAIALKPDTAANYNNLGNDLAKLGKVDDARAAYQKYVDLKPDDA
                     ALAWRNFGTVLYNSNRMKESIEPLQKATTLDPKNATAWLLLGIALVNTMEFKTVGDKI
                     TPVMQPGTVEAYQHAIDLDPNGPIGAQAKQGLESLQAMGVGITTKVGEAPPKSTKKK"
     gene            complement(3360..4109)
                     /gene="queC"
                     /locus_tag="VG272_00655"
     CDS             complement(3360..4109)
                     /gene="queC"
                     /locus_tag="VG272_00655"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HWF12218.1"
                     /translation="MRFTVASNNEEEALSGTMSRRPKAVVLLSGGMDSCVTAAIARNT
                     HELALVHASYGQRTERRELQAFTEIADYYGVRERLVVQLKHFAQIGGSALTDPRISVP
                     EGHFGAPVGGNEIPSTYVPFRNAHFLSVAVSWGEVIGATAIFIGAVAEDSSGYPDCRP
                     EYYRVFQQLVREGTKPETQIEIVTPVIGMRKWQIIQKGLELKAPLDRTWSCYQSEDAA
                     CGVCDSCRLRLSAFAEAGIGDPIAYRAQVER"
     gene            complement(4113..4820)
                     /locus_tag="VG272_00660"
     CDS             complement(4113..4820)
                     /locus_tag="VG272_00660"
                     /inference="COORDINATES: protein motif:HMM:NF024747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWF12219.1"
                     /translation="MIISEIFRSIQGEGTRTGLPCIFVRLTGCNLRCTWCDTAYAFHG
                     GEKMSVEEVLSRVSELSSHTAPKQNGSTRNQPKLPLVELTGGEPLLQPEAIPLSQKLL
                     DSGYQVMVETSGERQVSVLPREVIKIVDIKCPDSGEPDTFLMENLDALDGKDEIKFVI
                     SSRRDYEFARDFTLQHNLASRVHQVIFSPVHADPAGAWQGMNAQELVEWILQDELPVR
                     LGLQIHKFIWHPATRGV"
     gene            complement(4831..5016)
                     /locus_tag="VG272_00665"
     CDS             complement(4831..5016)
                     /locus_tag="VG272_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12220.1"
                     /translation="MNRSFLIVIVPAIAVGLGYLLMFHWLGFALEPFRFVGAAILFVG
                     AVILVHRRQRRKAKRGS"
     gene            complement(5039..5413)
                     /gene="queD"
                     /locus_tag="VG272_00670"
     CDS             complement(5039..5413)
                     /gene="queD"
                     /locus_tag="VG272_00670"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HWF12221.1"
                     /translation="MFQVSVDETFSSGHALRGYKGKCENPHGHNYKVQIVLEGPNLDS
                     IGLLYDFSHLKRVLRGIIEGVDHRYLNDQAPFDVLNPSAENLAKYFYDEIAGQLNAMP
                     AGARLARVTIWETDTATATYWV"
BASE COUNT         1238 a         1617 c         1472 g         1460 t
ORIGIN      
        1 catgatgcac cggccatccg tgctcattct cgacgaacca acgcgcagcc tcgatccgct
       61 cgcggcggct gacttccgaa aatttttggt tcgcgagatc gtgggcgcgc atgggacaac
      121 catgattttt gcatcgcaca cgctgacgga ggtagaacaa ctcgcggata agattgccat
      181 gctggacgac ggcagattgg ttgcctggga cacgctcgac agaatcaaaa gcgccacggg
      241 agtttcttcg ctggaagaat cgctcgaaat tctgacgcgc cacgtagtgc ggaaaaaacc
      301 gtgaacgatt cgctcaaatt aggcatgacc cttcgtaaac tcatgctaat ctttcgccgc
      361 gattttaccg tggcgcgcag ctatcgcttc gcttttgtaa tcaatctttt tcaggctttg
      421 ttcggaactg ccagtttcta ttttctttcc cgtttcattg aaagccccac gctgcagaaa
      481 tcgcttccgg ccggcacaaa ttatttttct ttcgtgctgg tggggatcgc gtttttcgat
      541 tacctgagcg tcgccctcag cacgtttgat tccagcctgc aggaagcgcg gcagaacggc
      601 acgctcgaaa atctcttggt gacacaaact tctcttccca taattctggc cggatcgagc
      661 ctctatccgt ttgccggaat gtcgctgcat acagtgatct atctcggctg gggcgcgctc
      721 ttatttggat ttccattgca cggagcgaat tggctgggcg cggtgttggt gctggcggct
      781 tccattttgg cgttctcggg tttcggcatt ttgagtgcgg cttacttgct ggtcttcaag
      841 cgcggcaatc cagtaaattg ggcaatcctg ggcctgtctt ccgtggtcgg cgggatgatg
      901 tatcccgttt ctgtgctgcc gaaatggcta cagtatgtgg cgagactcac tccagtcacc
      961 tattcgctgg aaggaatgcg cgcagcaatt ctcggccatg cctccttgcg cgaattgctg
     1021 ccgtcgatcg cgggattgtt tttattcgcg gccattcttc tgccaatttc cttcgctatt
     1081 ttttcctggg ccctgcgccg ggcgaaaatt accggaacgc ttactcattt ttaacaagca
     1141 atttgcttac ttttgcagga gtgacatatt ccgccaggac cattgacttt taactgtaca
     1201 gttcagataa tcgaactgga gagttcattt aaatgcccaa agccgcgcaa ggcacttcca
     1261 tcgttgtttc ggcgctccaa gcgagaacaa acttcgggcg gcttcttcgc cagatcgagg
     1321 aaaaaccctg ctcgctcgtg atcgaaaagc ggggcacgcc gagggcaatt ctcctcagca
     1381 tccgaaacta tgtgcggctc gccgcccccg aaccggaagt gctgaaagcg attggcaagc
     1441 aatccgaagg caaaggcacg gatgctcttt cttcgcgtca aatcgatcaa atcattaaag
     1501 cggcgcgcgc gaaaaaagct aagcgctgat gcttccactg cggctggttc tggacacgaa
     1561 tatcatcgtt tccgcggcga ttaaacccga aggattgcaa cgcacggtgc ttctgctggc
     1621 catgacgcgt cctgcacggt tgtatgtctc cgaggccttg ctttcagagt atcgaatggt
     1681 attgtcgcgt cccgaattca agatccgaaa aggtttacgc ctgcaattcc ttcagcgaat
     1741 caaagacgct tctcgcctgg catccccttc gcgccaaatt cgcgtcactc ccgatccaga
     1801 tgacaacata tttttggaat gcgcggacgc agcacgcgcc gattacctag ttacaggcaa
     1861 tctgcgccat tttccaaaat tttggaaaca aaccaaaatt gtctcttcac gtgaatttat
     1921 tacgatgatt acccctcacc ttgttccgta aatgttgcat ctccgcttcg cattaaaatt
     1981 aattttgggt attttgaggc ttgcgggaat aacaggcgag gttccatgac gggcaatgat
     2041 ttattgcttt tctgcgtaaa gaaacaaaaa tgcgcgatgg ggccagatca aatcccatcg
     2101 cgcatcgaaa ctgataaaag gtagttactt ctttttcgtt gacttgggcg gagcttcgcc
     2161 cactttcgtc gtgattccta cgcccatcgc ctgcaacgat tcaagaccct gcttggcttg
     2221 cgcgccaatc ggtccgttcg gatcgagatc gatggcatgc tgataggctt ccacggttcc
     2281 tggctgcatg acgggagtga tcttgtcacc cacggttttg aattccatcg tgttcacgag
     2341 tgcgattccg aggagcaacc aggcggtcgc attcttcgga tcgagcgtgg tggccttttg
     2401 cagtggctcg atggattcct tcatgcgatt tgagttgtag agcacggtgc cgaaattgcg
     2461 ccatgcgaga gcggcgtcgt ctggtttcag atcgacatat ttttgatacg cagcgcgggc
     2521 atcgtccact tttccaagtt ttgccaggtc attgcccagg ttgttatagt tagcggccgt
     2581 gtccggtttc agagcgatag ccttctgata ggtatccgca gcgtcttgcc atttgcccat
     2641 gctctcgtag gcttcaccaa ggcgggacaa aataatggag cggttcggat cttccggctt
     2701 ctcgagttcc agcgcggcgg acaattccgt gacggcggtg cctccagcct gagtcacttg
     2761 cgcctttatc ggatcctgct gctctttcgg agtctgcgcc agtttcgttt gcgcggcctt
     2821 catctgttcg atggccgcga tacccgcatc atagtgcgtc ttcagggcag cgaattgggc
     2881 ttccgcttct tgccgcttgc gttcggcttc gacggcggct agctttcctt gctcctggag
     2941 ttccttgaag ttgatgtcaa acgtaggaga agtgtttggc gcaagcttca atcctgtctg
     3001 gtagagcaat tcatctttct ggtccttatc cttgcctgtc acgtcaacgt tataggttcc
     3061 gccggccaac cctggcgcgg aataatgccc tttgtcatcg gtagtgacct ttgtttcttt
     3121 attcgtttca acgctcttca ttgttagcgt gacgttgggg tatggcttgc cggtaagatc
     3181 caatactgtg cccgacaacg tggcattttg cgcgaaggcg gcaggcgcga gcaacaccgc
     3241 caatgggccg gcgatcagcg caagcataat tgccttcctg attactttca ttcggttcat
     3301 gatcttcact gcctcctggc aacgcagatc cgcactggga ttacccgcgc gggttattat
     3361 tatctctcaa cttgggcccg ataagcgata ggatcgccga tccctgcttc ggcaaaggcg
     3421 ctgagcctaa gcctgcaact atcacaaaca ccgcatgccg catcttcaga ctggtaacag
     3481 gaccatgttc ggtccagcgg cgcctttaat tccaaaccct tctgtatgat ctgccatttt
     3541 cgcataccaa tgaccggagt aacaatttcg atttgtgttt ctggtttcgt accttcgcgc
     3601 acaagctgct ggaagacacg gtagtattcg ggcctgcagt ccgggtagcc ggaactatcc
     3661 tccgctacgg cgccaatgaa tattgccgtt gcccctatta cctctcccca gcttaccgca
     3721 acggaaagga aatgcgcgtt acgaaatggg acgtatgtgg agggaatctc gttcccacca
     3781 actggagcac cgaagtgccc ttctggcacg ctgatacgtg gatcggtcaa agccgagccg
     3841 ccaatttgcg cgaaatgttt taattggaca actaggcgtt cgcgtacacc gtagtaatct
     3901 gcgatttccg tgaatgcctg cagctcgcgg cgttccgtgc gctgcccgta gctggcgtgg
     3961 accagggcta actcatgcgt gttccgcgcg atggcggcgg ttacgcagga atccatgccc
     4021 ccgctcagca acacgactgc cttgggtctc cgactcatgg ttccgcttaa cgcttcctct
     4081 tcattattag atgcaacagt gaaccgcaga gtctacacac cgcgggttgc cggatgccag
     4141 atgaatttgt gaatttgtag tcccaggcgt accggcagct catcctgcaa aatccattcc
     4201 acgagttcct gcgcattcat tccttgccag gcgcccgcgg gatccgcgtg aaccggagaa
     4261 aaaatcacct ggtgcacgcg gctcgccaga ttgtgctgca acgtaaaatc gcgcgcgaat
     4321 tcataatccc gtcgagatga gatcacaaat ttaatttcat ccttgccatc gagcgcgtcc
     4381 aaattttcca ttaaaaacgt gtctggctct ccggaatccg gacatttaat atctacaatt
     4441 ttaatcactt cgcgcggaag cacggatacc tgccgttcgc cgctggtttc caccatcacc
     4501 tgataaccgc tatctaaaag tttttgcgaa agcggaatcg cttccggctg aagcaagggc
     4561 tcgccgccag tcaattcaac aagcggaagt ttaggctgat ttcttgtgct tccgttttgc
     4621 ttcggggcag tatgcgagga caattcactc acgcgtgaca aaacttcctc gacgctcatt
     4681 ttttcgccgc catgaaatgc gtacgccgtg tcgcaccacg tgcagcgcag attgcatccg
     4741 gtgagccgca cgaaaatgca tggcaggccg gtccgcgtgc cttctccctg aatcgaacga
     4801 aatatttccg aaatgatcat gacgccgcta tcagctcccg cgcttggctt tccggcgctg
     4861 gcgcctgtgc acaagaatca cggcgccaac aaacaaaatt gccgcgccga cgaagcgaaa
     4921 tggctccagc gcaaacccca gccagtgaaa catcagcagg tatccgaggc ccacggcaat
     4981 ggccggaacg atcacgatga ggaatgagcg attcatggaa ataaattatg cgttgcgatt
     5041 aaacccagta cgtcgccgtt gcggtgtcgg tctcccagat ggtgacgcgc gccaaccgcg
     5101 ccccggcggg catggcattc aactgcccgg caatttcatc ataaaaatat ttggcaagat
     5161 tctccgccga tggattgagc acgtcaaacg gagcctgatc gttcaaatat cggtggtcca
     5221 ctccctcaat aatgccgcgc agcacgcgct tcaaatggct gaaatcgtaa agcagaccga
     5281 tggaatcgag gttcggccct tccaggacaa tttgaacttt ataattgtgg ccgtgcggat
     5341 tctcacactt ccctttatag ccgcgaaggg catgtccgga agaaaaagtt tcgtcaacgc
     5401 tgacttgaaa catcgattta cccttttaat gaatttccaa tggcaataat tgtgcggcgt
     5461 ccccgcaaaa aacccccgcc tctgaccctt cgcgttctct cagggcgaga gaggcgggct
     5521 acaattgctg tagctcgggt cttcagaccc gaggcctttc tgccccgcca gagaatattt
     5581 ccccgcacac cccggcgcaa accgcccatc cccacgcgct atttcgattt ccgcatgaac
     5641 cgttcaactg cggcgatgtc cctggtgttc gagtacgccg gaagcgattc catgaagatt
     5701 ttaccatatt gcatgcgctg gattcggtta tcgaggatgg ccagcacgcc gcgatccgtg
     5761 cgcgaacgga tgagccgccc gaagccc
//
LOCUS       DASZMB010000018         6175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1113311, whole genome shotgun sequence.
ACCESSION   DASZMB010000018 DASZMB010000000
VERSION     DASZMB010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113311"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VG272_00675"
     CDS             complement(<1..588)
                     /locus_tag="VG272_00675"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HWF12222.1"
                     /translation="MASESRASSTSRVTVAPSGKALGAEIQRVDLRAVSADDFESIHR
                     AWLDHSVLLFRNQKLTDDDLIAFSKHFGDLDLAPIQETGRRFVEGHPEIYVVSNVMEN
                     GVPIGSLGAGEATWHTDMSYLPDPPKASILYALEVPPSGGNTYFSSMYRAYEILPNAL
                     KKRVEGKLLKHDGTYNSGGYVRQGVTAVDDPVQSPG"
     gene            complement(619..1083)
                     /locus_tag="VG272_00680"
     CDS             complement(619..1083)
                     /locus_tag="VG272_00680"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWF12223.1"
                     /translation="MKLQFALIFAVIVILAVVYGCGKSEKSGASSSSGPTKVSGSPTT
                     TPSGLEYWDVVVGTGPTAVPGNMVHVHYSGYLTSGEKFDSSRDRGEPFSFPLGAGQVI
                     KGWDEGVAGMKVGGQRQLRIPPELGYGSAGAGGTIPPNATLVFDVELLGVDK"
     gene            1345..2355
                     /locus_tag="VG272_00685"
     CDS             1345..2355
                     /locus_tag="VG272_00685"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HWF12224.1"
                     /translation="MKNSLGTFSLLALLGGCALIPRVWADTLAVVCKSDFQLELVDPA
                     TEKILLKLPTGLGPHEVAVSPDGRTAYVSNFGRYSVYPAGDTAHDKAGNTITVIDLVD
                     RKVKSTFDLGTHTGPHGMIVSRDGKLIWVTTETPQAVLELDSASGKILHVWNTNQLRS
                     HMIVTTPDEKKFYVTNTVSGSVSVIDKTTGEVKIVSTGPGTEGIAISPDGKEVWAASR
                     IDAKISIISTATDGIIAAFRSGGKGPKRMAFTPDGAQIWVTNSASNQTTVFDAHAREL
                     IAALDMAKAPSSLYFSGDGRRVFVTNANANEISFVDVASRKILTVMPIGTDPDGVIWS
                     SQ"
     gene            complement(2462..3481)
                     /gene="aroF"
                     /locus_tag="VG272_00690"
     CDS             complement(2462..3481)
                     /gene="aroF"
                     /locus_tag="VG272_00690"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWF12225.1"
                     /translation="MIVNMSATATKEQVEHVMDRIKECGFLPHLIQGAERTVIGVVGK
                     NRGRRAEVEALKVAPGVEDIISISQPFTLVSLELQPDRTIVDVSGVKIGGPELIVMAG
                     PCSVESRDQLMQTARGVKAAGAHLLRGGAYKPRTSPYDFQGLGVEALKIMAEAREETG
                     LPIVTEVMGIEDLDAVVEYADMLQVGARNMQNYPLLRKLATVPRPILLKRGPSATIKE
                     WLLAAEYLLSGGNPNVVLCERGIKTFDAELRNTLDLAGVALAKELCHLPIVVDPSHAT
                     GRRSLVPAAARAAVAIGADGIIVEVHPCPERALSDGPQSLDLPGFAAMMKGLQPAIAM
                     VAAGR"
     gene            complement(3974..4672)
                     /locus_tag="VG272_00695"
     CDS             complement(3974..4672)
                     /locus_tag="VG272_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWF12226.1"
                     /translation="MPPNPEVDFIHKMASRMAAADPLHEVLSQIVDFAASVVSCDSCF
                     LYALEEGELILRSSKNAHPDVVDRLKLRLGQGITGWVAEHGKPVAVAVNASQDPRFQL
                     FKDLPEDRFEAFLSVPVMCRGRIVGVINLQNREPHVYTEREIMLVTTIGFLVGAEIEM
                     ARLESENLHLSEKLETRKLVERAKGILQRELKLTEEEAYLTIQRQSRQRRKSMKEVSE
                     AIVLSEDVRRGMGE"
     gene            complement(4939..5814)
                     /locus_tag="VG272_00700"
     CDS             complement(4939..5814)
                     /locus_tag="VG272_00700"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HWF12227.1"
                     /translation="MLTMENENSPKNFAGLRVLSLESRRGPEMAKLITTYGGQATVAP
                     SMREVPLESNTEALSFARTLFAGGFDTVIFLTGVGTRTLAKVVETAQPLESYLAELRK
                     ITIVARGPKPLTVLREWNVPVAVTAPEPNTWRELLRALDENAEAVPLKGIRVAVQEYG
                     VSSKELIAGLAERGAHVTSVRVYEWALPEDTGPLRSAVTAISRNELDVILFTTATQAD
                     FLLQVAAEMKEEGSLRRALSRMAVVSIGPTTSERLREHGIAPDMEPTHPKMGYMVSEA
                     AQQSAEILQRKRISR"
     gene            complement(5947..>6175)
                     /locus_tag="VG272_00705"
     CDS             complement(5947..>6175)
                     /locus_tag="VG272_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12228.1"
                     /translation="PDETRGLVPGLAYQLGILIAAPTNSVELLLQEHVGYQWALAGFE
                     IVTIGVLIITIALGKERKGRSFHEPVATVAS"
BASE COUNT         1372 a         1682 c         1697 g         1424 t
ORIGIN      
        1 gcccggagat tgaacgggat catcgaccgc agtcactccc tgccgaacgt agccgccgct
       61 gttataagtt ccatcatgct tcagcaattt gccttcgaca cgcttcttca gcgcattggg
      121 caggatttcg taggcgcgat acatgctgga gaagtacgtg ttgccgcctg atggaggaac
      181 ttccagcgcg tacaggatgc tcgccttcgg cggatcggga agataggaca tgtccgtatg
      241 ccaggttgcc tcacccgcgc ccaggcttcc gatgggcacg ccgttttcca tcacgttgga
      301 caccacataa atttcgggat gtccctcgac aaagcggcgg cccgtttctt gaattggagc
      361 caaatccaag tctccgaagt gtttgctgaa ggcgatgaga tcgtcatccg tcaatttctg
      421 gttccggaaa aggagaacgg aatgatccag ccaggcccgg tgaatgctct caaaatcgtc
      481 agcactcact gcgcgcaaat cgactcgctg aatttcggcg cctagagctt ttcccgaggg
      541 agcaactgtg acgcgagaag tgctgctcgc tcgtgactcg cttgccattg aaatttctcc
      601 cttgttattt atccctcgtt atttatccac gccgagcaat tccacatcga agactaaggt
      661 cgcgttcggc ggaatcgtgc cacccgcacc ggcagatccg tagccaagct ccggggggat
      721 tcgcagttgc ctctggccgc caactttcat ccctgcaacg ccttcatccc agcctttgat
      781 gacctgtcct gcgccgagcg gaaatgaaaa tggctcgccg cgatcgcgag aactgtcaaa
      841 tttttcgccg ctggtcaggt agccgctgta gtgcacatgg accatgtttc caggaaccgc
      901 cgttggaccg gtgccaacca caacgtccca atattccagg ccggaaggag tggttgtggg
      961 tgagccgctt actttcgtcg gaccagagga gctggacgcg cctgactttt ctgatttccc
     1021 acagccgtat acaaccgcaa gaatcacgat gacagcaaag atcaacgcaa attgtaattt
     1081 cattattccc ctttcgattt acagaatcat atcaggagtg ttgatatgga ggtcgaagaa
     1141 atacaccgcg cataatttta tctgatttca actttctttt gctgcaatcc agtagcacag
     1201 gcctcagcct gtggggtttc gtgtttgcaa gacctcaatt ccacaggctg aagcctgtgc
     1261 tactggattg acataggaca tggaccggag tacggtcgcg agacgcagcc agggcctatc
     1321 tcggatattt caggagaaat cagtatgaaa aacagcttag gaacattttc gctgctcgca
     1381 ttgctcggcg gatgcgcgct tattccgagg gtgtgggctg acacgctggc cgtggtttgc
     1441 aaatccgatt tccagttgga gctcgtcgat ccggcgacgg aaaaaattct gctgaagttg
     1501 ccaactgggt taggcccgca cgaagttgcg gtttcgccgg atggccgcac cgcgtacgtt
     1561 tctaactttg gccgatacag cgtctatccg gcgggagaca cggcgcacga caaagcgggc
     1621 aacacgatca ctgtgatcga tctcgtcgac cgaaaagtga aatccacttt cgatctaggc
     1681 actcacacgg gaccgcacgg aatgatcgta agccgcgatg gaaaattgat atgggttacg
     1741 accgagactc cgcaggctgt gttggagctg gactccgcca gcggaaaaat tctgcatgtg
     1801 tggaatacga atcagctgcg cagccacatg attgtgacga ctccggatga aaaaaagttt
     1861 tacgtcacga atacggtttc gggcagcgtc agcgtgatcg ataagaccac gggcgaagtg
     1921 aaaattgttt cgactgggcc gggcactgag gggatcgcga tttctccgga tggaaaagaa
     1981 gtgtgggctg ccagccgaat tgacgccaaa atttcgatta tctcgacagc tacggatgga
     2041 atcatcgctg catttcgcag cggaggcaaa gggcctaagc gaatggcgtt cacgccggat
     2101 ggggcgcaga tctgggtgac aaattccgcg agcaatcaga caacagtgtt cgatgcgcac
     2161 gcgcgtgaac tgattgctgc tcttgatatg gcaaaagcgc cgagcagcct ttatttttct
     2221 ggggatggcc gccgcgtatt cgttaccaat gcgaacgcga atgagatcag ttttgtggat
     2281 gtagcgtcgc ggaaaatttt gacggtgatg cccattggca cagacccgga tggggtaatt
     2341 tggtcgtccc agtaaagtga cagccctatg tggcataggc ttcagcctgc ggggtttgtc
     2401 cttgcaagga ctaaattcca caggctgaag cctataccac gaaaactcaa ttccgccagc
     2461 gctaacggcc ggcggcaacc atggcgatgg cgggctgaag gcccttcatc atggcggcga
     2521 aacccggtaa gtcgagcgat tggggaccat cggacaaagc gcgctcgggg caaggatgca
     2581 cttcgacaat gatcccgtcg gcgccaatgg cgaccgcagc gcgcgcggca gcaggaacca
     2641 ggcttcggcg tcccgtggcg tgcgacggat cgactacaat tggcaagtgg caaagttcct
     2701 tggcgagagc gactcccgcg aggtccaaag tatttctcag ctcggcgtca aatgtcttga
     2761 ttccccgctc gcacaaaacc acgttggggt taccgcctga cagcaaatat tcagcggcca
     2821 gcagccattc tttgatggtg gccgagggac cgcgttttag caggattggc cgcggcacgg
     2881 tggcaagctt tcgcaagagc gggtagttct gcatattgcg cgcgcccact tgcagcatat
     2941 cggcgtactc aaccacggcg tcaagatctt cgatgcccat cacttccgtg acaatcggca
     3001 agccggtttc ctcacgcgct tccgccatga tcttcagcgc ttccacaccg aggccctgga
     3061 aatcgtaggg cgaggtgcgc ggcttgtacg cgccgccgcg aagcaagtgg gcaccggcag
     3121 ccttcacgcc gcgcgccgtc tgcatcaact gatcgcgcga ttcgaccgag cacggcccgg
     3181 ccatcacgat cagctcgggc ccgccgatct tgacgcctga cacgtcaacg atagtgcggt
     3241 cgggctgcaa ctcgaggctg accagcgtga acggttgcga aatggaaatg atatcttcca
     3301 ctccgggcgc gacttttagc gcttcgactt cggcgcgacg cccacgattt tttccgacca
     3361 cgccgatcac ggtgcgctcc gcaccttgaa tcagatgagg aaggaatccg cattctttta
     3421 ttctgtccat aacgtgctcc acttgttctt tcgttgccgt tgccgacatg ttgacgatca
     3481 ttacgagtct cctttggctt cctgctgtgt agcgagctct taaggaagcc cgcattacca
     3541 tcagtcaaaa ataaaaaagc cgcgggaccc ttggggttct cgcggccagt tccgggattc
     3601 tttcgcgctt tctaactttt cgctttacct cgtcgtgcgc gcgatccgcc cctggccgct
     3661 tcccacccag gggaagcggt acgtaaaggc gaaccaataa taagagttag agtgcattgc
     3721 gagtgtgaga atacgcagct aggagcctgt tgtcaagttt tttggcagga aggctgggtg
     3781 cgctaaacgc ggcggatttc aacacagagg gcacagagaa aagaagagaa aattacgaga
     3841 aattaaacca acgagtaaat aattctaggc cttccatttt gtctgaggtt ttctcctctt
     3901 ttctctgtgc cctctgtgtt aaagcttttg acttttcgct ttggaaatga tgtaacgggc
     3961 acgctgcggc ccactattcc cccatgccgc gccgcacgtc ttcgctcagc acgatggcct
     4021 cggaaacttc cttcatcgat ttgcgacgct gacgactctg gcgctgaatg gtgagatagg
     4081 cctcctcctc ggtcagcttg agttcacgct gcaaaattcc cttggcgcgt tccactaatt
     4141 tgcgtgtctc cagtttttcc gaaagatgca gattttcgct ttcgaggcgc gccatctcga
     4201 tctccgcgcc gactagaaaa ccaatcgtgg tgaccagcat aatttcgcgt tccgtgtaga
     4261 cgtgtggctc gcgattctgc aggttgatga cgccgacgat tcggccgcga cacatcacag
     4321 gcacggataa aaacgcctca aaacggtctt ccggtaaatc cttgaataat tgaaagcgcg
     4381 ggtcctgaga ggcgttaacc gcaacggcga ccggcttgcc atgttcggct acccatcccg
     4441 taatgccctg gcccaggcga agcttgaggc ggtctacgac atcgggatgc gcatttttgg
     4501 atgagcgcag gatcagttcg ccttcttcga gcgcgtataa aaagcaggaa tcgcaactca
     4561 caacggaagc ggcaaaatcg acgatctggg agagcacttc gtgcaaaggg tcggcagcgg
     4621 ccatccggct ggccatctta tgaataaaat cgacttccgg attcgggggc atatcaactt
     4681 tgctcttctc ggtattcacg attcaaggct acgcgcgggg gcgacgagtg tccaatttgt
     4741 cctatggatg tgaaccatca gtctttgctt gatgtaacac tggagaaata aatcgcttcg
     4801 tggcgccggc atcttgccgt cggtttttca ttttttggaa atttcaagga agacccgccg
     4861 gcgggacacc ggcgccacga agcctgggct ttgcacttgg aattatgcga ccgcgacttt
     4921 cctgcatcgt tacttttact acctcgaaat tcgttttcgc tgcaaaattt cggcgctttg
     4981 ctgcgcggct tcgctcacca tgtatcccat tttcggatgc gtcggctcca tatctggggc
     5041 gataccgtgt tcgcggagcc tttcgctggt tgtgggacca atcgagacta ccgccatgcg
     5101 cgaaagcgcg cggcgaaggg agccttcttc cttcatttcc gcggcaactt gcaggagaaa
     5161 atcggcctgc gtggcggtcg taaagaggat tacatccaat tcattgcgtg aaattgcggt
     5221 aacggcggaa cgcagcggtc cggtatcttc aggcaaagcc cattcataaa cgcgaaccga
     5281 agtgacatgg gcgccgcgct cggccaaacc cgcaatcaat tccttgctgg agacaccata
     5341 ttcttgcact gccactcgga tacccttcaa agggactgct tccgcgtttt cgtccagagc
     5401 gcgcaataat tcgcgccacg tatttggttc gggggcagtg acggctaccg gaacattcca
     5461 ttcgcggagc accgtcaaag gcttcggtcc gcgcgcgaca attgttattt ttcggagttc
     5521 cgccaaataa ctctccagcg gctgagcggt ctccacaact tttgcaagag tgcgtgttcc
     5581 aacacccgtc aggaaaataa ccgtgtcgaa gccgcctgca aaaagtgtgc gagcgaagga
     5641 cagcgcttcc gtgttgcttt cgagtggcac ttcgcgcatg gaaggtgcaa ccgttgcctg
     5701 gccgccatag gtggtgatca acttggccat ttcagggccg cgccggcttt ccagtgagag
     5761 gactcgcaga ccggcaaagt tttttggtga attttcattt tccatagtta gcatgatgcc
     5821 gcaactgtaa gaaattctct caaagaaatt ttggggatgg attatgtggc ataggcttca
     5881 gcctgtgggg ttaagttctt gctagcacta aattccacag gctgaagcct gtgccacgaa
     5941 aagcaatcag gaggctactg tggctacggg ttcgtggaag ctacggcctt tgcgttcctt
     6001 gccgagcgcg atggtgatga tcagcacgcc gatcgtgaca atttcaaatc cggctagtgc
     6061 ccattgatag ccgacatgct cctgcaacag aagctcaacc gaattggtcg gcgcggcaat
     6121 taaaattccc agttgatagg ccagtccggg aaccaagccg cgcgtttcat ccggt
//
LOCUS       DASZMB010000019         7287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_290138, whole genome shotgun sequence.
ACCESSION   DASZMB010000019 DASZMB010000000
VERSION     DASZMB010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7287
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290138"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            375..1265
                     /locus_tag="VG272_00710"
     CDS             375..1265
                     /locus_tag="VG272_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWF12229.1"
                     /translation="MSSRFKAHCALMACSFFWGVTFVVVKNALADISVFAYLSARFML
                     GALPMIWIYRNDLRNLTRDEAWAGVQVGIFMAAGYAFQTAGIARTTPSKAAFITGLSV
                     VLVPIFLAAFWRRKIGAWAWGGAAGSFAGLYFLTVPPAGFSQLNHGDMLVMGCAVLYA
                     LQIIYIARFTGKYSLGALSCLQVILTGVVSTLAVPVLNFTGLEHFFVRFTPQMEFGVL
                     VTATFTTALAYPMLVWGQTHTTATNTALILTSEPVFAAITSFVLLHERLGGRALAGAI
                     LILGGIALAEWKGTVPSAGS"
     gene            complement(1350..4433)
                     /locus_tag="VG272_00715"
     CDS             complement(1350..4433)
                     /locus_tag="VG272_00715"
                     /inference="COORDINATES: protein motif:HMM:NF017078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF12230.1"
                     /translation="MTVRRKTILIISLTCLGLVAVLYGASRGIMRGGFIQIEQTLAQE
                     NIQRMVNVLNQDVGAIDRFTYDRASIEETYYGMSTQSPELLHWLMGKDDSGTVQTQRL
                     NFVILIGTSGNVIESRGFDRNTKEVVPIPGTLMAHLSPNDPLIQTAASAGKTAGILVL
                     PEGPLLIVCRPVVRPGTTDAAHGYMVSARFLDGTGDIKDLIKLVNFPLSLQLIDTKYL
                     PEDFSEARSHLFKDGDIYTQPLSESVLGGYTLLNDIYGKPALILKAEMPRRMYAQGKK
                     SQLYFVGSLILAGVVFGSVVLLLVDRTVIYRLNNFSQSVISIANSGEPAKRVNCSGSD
                     EISALGNAINSMLASLQLAQWQRQRVEGRYKAFMNTIPAVASIKNQEGRFLYVNEPMR
                     QYYGIQAGDALGSTTAECLPTAIAQVMHRDDQEVISTGHPMQFERVVPTPDGVDHHWL
                     TFKFRIDEPDAQMLVGTIAIDITDRKRAEAEAQKAKEVAEAANRSKSEFLANMSHEIR
                     TPLNGVIGMTDLALGTDLTAEQQEYLQIVKLSADSLLVVINDILDFSKIEAGKVELEK
                     VDFNLHELVEMTMKTLALRAEEKGLELLCDFSPDVPDSVQGDPTRLRQIILNLVGNSI
                     KFTAEGEVQLRVALLPDNSTAPFLQFTVTDTGIGISREQQKTIFEPFTQADSSTTRKF
                     GGTGLGLSISMHLVRMMGGEMWVESETGSGTKFHFKLPLVPAANPVEIRDQPASALLL
                     GARVLIVDDNATNRQILQLIVRRWEMAPTVANSATAAILELKAAVAAARPFDLVLSDL
                     LMPGVDGFAFTEQVRTIPEIANTKIIMLSSAGRRGDAKRCERLRISAYLMKPVRQMEL
                     KTVILTLLGEPAPSQNGDVLTRFAMKNVSSVPKRLRILVAEDNAVNQKLIVRLLEKRG
                     HSVHVVSNGLEAVDLLETCKFDLVLMDMQMQVMDGFEATRKIRKRETSDGNHLPIVAL
                     TAFAMKGDRERCLDAGVDGYLTKPIRAAELDEVLSQYISSRPETLYSGQIEKISS"
     gene            complement(4915..5553)
                     /gene="leuD"
                     /locus_tag="VG272_00720"
     CDS             complement(4915..5553)
                     /gene="leuD"
                     /locus_tag="VG272_00720"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HWF12231.1"
                     /translation="MQTFSKHTGVVLPFDRVNVDTDQMVPKQFLKLQTKDGYGKVLFY
                     DWRYLPDETLNPDFIMNWPRYKGVSILLARAGFGSGSSREHAPWAIADYGFRVLIAPS
                     FADIFYSNCFKNGILPIVLPEAQIDELFRRAESQEGYSLTVDLPAQTISDASGLLYKF
                     EIAEFRKHLLLEGLDDIGLTLKHEIDISAFEESHDPGATLYSPVDSKYAVPQ"
     gene            complement(5672..7093)
                     /gene="leuC"
                     /locus_tag="VG272_00725"
     CDS             complement(5672..7093)
                     /gene="leuC"
                     /locus_tag="VG272_00725"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009116.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HWF12232.1"
                     /translation="MNPRTMYRKIWESHLVREAEGEPSLIYIDRHLIHEVTSPQAFAG
                     LKAAGRKVRRPDLTFAVMDHSVPTKDRNLPILDKAAGGQFDALERSCRENGITLFDMF
                     SHNQGIVHVIGPELGITQPGQTLVCGDSHTSTHGAFGTLAFGIGTSEVEHVLATQTMQ
                     QYESKTMNIVVNGKRTQGVSAKDIILAIIGKIGIGGGAGHVFEYAGEAIRALSMDGRM
                     TVCNMSIEGGARAGMIAPDDTTFAYMEGRPYVPRGKEFQDAVQNWKALASDPGATFDT
                     TVELDASALAPQVTWGTNPGMVAGVTDRVPDPGAFRDPDDRKAVERALTYMGLTPGTA
                     LVDVPVDRVFIGSCTNSRINDLRAAARVVAGKHVAKNLKGALVVPGSMTIKEKAEAEG
                     LDKIFRDAGFEWRDAGCSMCIAMNPDVLLPGERAASTSNRNFEGRQGKDSRTHLVSPI
                     MAAAAAIAGHFVDVRDWDVEATE"
BASE COUNT         1549 a         2083 c         1890 g         1765 t
ORIGIN      
        1 cttttctttc ttttccttct ccggtttttt gtctttattt ttttctttat ccgcggcatg
       61 caaactggaa cacagcaatg cagcaaagat gaacagtgtc gcaggagcaa tctttatcag
      121 gatgggatgg cgctttgagg caatcattct tgggtggcaa ctagccgttt ctccgtaagt
      181 ttaccagaca agccttccgg caggcgccag aatcgtcaat ggtgcgattc gtttaacgtc
      241 tggcaggttg cctttcgtcc atcgaatcaa tagactcgaa cgcttgcatg gctgccgaaa
      301 atttttcaag aagattgaat accggtccaa ttgacacacg attaccaagt tttaattaag
      361 cgcgggcgag cgcaatgagc agccgtttca aagcccattg tgcccttatg gcctgttcct
      421 ttttctgggg tgtcaccttc gtcgtcgtca aaaacgcgct tgcggacatc tccgtattcg
      481 cttatctctc cgcaaggttc atgctgggcg cgctgcccat gatctggatt tacaggaatg
      541 acttgcgcaa cctcacccgc gacgaagcct gggccggcgt ccaagtcgga atttttatgg
      601 ccgcgggtta tgcgtttcaa acggcgggaa tcgcccgcac caccccttcc aaggccgcgt
      661 tcatcacggg gctcagcgtc gtgctggtcc ccatattttt agccgcgttc tggcgcagga
      721 agattggcgc atgggcttgg ggaggggcgg caggatcgtt tgccggactc tattttttaa
      781 ccgttccgcc cgcgggcttt tcccagctca atcacggaga tatgctagtg atgggctgcg
      841 cagtcctcta cgcgctgcaa atcatttaca tcgcgcgatt cacaggaaaa tactcattgg
      901 gcgcattgag ctgcctgcaa gtgattctca cgggcgtcgt gtcgactctt gcggtgcccg
      961 ttctgaattt cacggggctg gaacattttt ttgtgcgctt cacgccgcag atggagtttg
     1021 gagtgctcgt caccgcaact ttcaccactg ctctggcgta tccgatgctc gtgtgggggc
     1081 aaacgcacac gacggcaacc aacaccgctc tcattttgac ttcggagccc gtgtttgcgg
     1141 ccattacgtc cttcgtgcta ctgcatgaga ggctgggcgg acgggcgctg gctggcgcga
     1201 tcctaatttt gggcggcatt gcgctcgccg agtggaaggg tacggtgccg tccgccggat
     1261 cgtaattccc gacgagccgc atggtctgga agaaaatcga agatcaacga gcgaagagat
     1321 cagcattacg tcaacaagtg gaaacttagt cagctggaaa tcttttcgat ctgcccgctg
     1381 tacaaagttt ctgggcgtga tgaaatatat tgactcaaaa cttcatcgag ctcagccgcg
     1441 cgaatcggtt tcgtaaggta tccatccacg cccgcatcga ggcatcgctc ccggtctcct
     1501 ttcatcgcga atgcagtgag ggcgacgatc ggcaaatggt ttccgtccga agtttcgcgc
     1561 ttgcgtatct tgcgggtggc ttcaaatccg tccatcactt gcatctgcat atccatgaga
     1621 accaaatcaa acttgcacgt ttccagcagg tcgaccgctt cgagcccgtt tgaaaccaca
     1681 tgaaccgagt ggccgcgttt ttccagcagc cgaacaatca gtttctggtt taccgcgtta
     1741 tcttccgcga ctagtatccg cagtcgcttc gggacgctag aaacattttt catggcaaat
     1801 ctggtcagga catcgccgtt ttggctcggc gccggctcgc ccagaagggt taaaatgaca
     1861 gtcttgagtt ccatttgccg caccggtttc atcaggtacg cactaattcg cagcctctcg
     1921 caccttttcg cgtctcctct ccgcccagcg gaactcagca tgatgatttt tgtgttggcg
     1981 atttccggaa tggtcctgac ttgctccgtg aatgcgaacc cgtccacgcc tggcatcaac
     2041 agatcgctca ggaccaaatc aaatggccta gccgccgcca ccgcggcctt cagttcgagg
     2101 atggcggccg tcgcgctgtt ggcgacggtc ggcgccattt cccagcgcct cactatgagt
     2161 tgcagaattt gccggttcgt tgcgttgtcg tccacgatca gaactctcgc gccgagcaac
     2221 aacgcagaag ccggctgatc tctaatttcc accggattcg cggcgggtac gagcggcagc
     2281 ttgaaatgaa acttcgttcc acttcccgtc tctgattcca cccacatttc tccacccatc
     2341 atgcgcacca gatgcatgga gatggacagg cccaggcctg tgccgccaaa ttttcgcgta
     2401 gtcgatgaat cggcctgcgt gaagggttca aagatggttt tttgttgctc gcgcgatatt
     2461 ccaatgcccg tgtcggtaac cgtgaattga aggaacggtg ccgtgctatt gtctggcagc
     2521 agagcgactc gtagctgaac ttcgccctcg gccgtgaact tgatcgaatt tcccaccaga
     2581 ttcagaataa tctggcgcag tcgggtcggg tcgccctgga ccgagtccgg aacatcaggc
     2641 gaaaaatcgc aaagcagctc cagtcctttt tcttccgcgc ggagtgctag cgtcttcatg
     2701 gtcatttcca cgagctcatg aagattgaaa tcgacttttt cgagttctac ttttccggct
     2761 tcaatttttg aaaaatccag aatgtcgttg atcacaacca gaagagaatc cgctgaaagt
     2821 tttacgatct gcaggtattc ctgctgctcg gcggtgagat ctgtacccaa tgccaggtcc
     2881 gtcatgccga tcacgccgtt cagcggcgta cgaatttcgt ggctcatgtt ggccagaaat
     2941 tcgcttttcg accggttagc cgcctccgcc acttccttcg ctttctgtgc ttccgcctcg
     3001 gcgcgcttgc gatctgtgat gtcaatggcg atggttccca ccagcatctg tgcgtctggc
     3061 tcgtcgatac gaaatttgaa cgtgagccaa tggtgatcca caccgtcggg cgtggggact
     3121 acgcgctcga actgcatcgg atgcccagtg gaaattacct cctggtcgtc ccgatgcatg
     3181 acttgcgcaa tcgctgtcgg caagcactcc gcggtcgtac tccccaacgc gtcgccagcc
     3241 tgaatgccgt aatactgcct catgggttca ttcacgtata aaaagcggcc ttcctgattc
     3301 tttatggagg ccactgccgg aatcgtgttc atgaaggctt tgtagcgccc ctccacccgc
     3361 tgacgttgcc actgggctaa ctgtaaggac gccaacatgc tgttaatcgc atttcccaag
     3421 gctgaaatct cgtcgctacc cgagcagttc acgcgcttgg cgggttctcc gctgttggcg
     3481 atggaaatca cactttggct gaagttattt aatctgtaaa ttacagtcct atctaccagc
     3541 aacagaacga ccgagccaaa cacgaccccc gccagaatca gcgaccccac gaaatataac
     3601 tggctctttt ttccctgcgc gtacattcgc cgcggcattt ccgctttcag aatcagcgcg
     3661 ggctttccgt aaatatcatt cagcaacgtg tagcctccca aaaccgattc gctcagcggc
     3721 tgggtgtaaa tgtcgccatc cttaaacagg tgactgcggg cttccgaaaa gtcctccggc
     3781 aagtacttgg tatcgatcag ctgaagagag agcggaaaat tgacgagctt aattaaatct
     3841 ttaatgtcgc cggttccatc cagaaagcgc gcagagacca tgtatccgtg agcggcgtcg
     3901 gttgtgcccg gtctgacgac tggacggcaa actatgagca gcgggccctc cggaaggaca
     3961 aggattcctg cagttttccc cgcgctggcc gctgtctgaa tgagagggtc gtttggcgag
     4021 agatgcgcca tcaacgtgcc cggaatcggc actacttctt ttgtgttgcg gtcgaatcct
     4081 ctggattcga tcacgtttcc ggaggtgccg atgagaatca cgaaattcaa tcgctgtgtc
     4141 tggacagttc cggaatcatc ttttcccatg agccaatgaa gcaattccgg cgactgtgtg
     4201 gacatcccgt aatacgtttc ctcaatcgat gcacggtcat aggtaaagcg atcgattgcg
     4261 ccgacgtcct ggttaagcac gttaaccatt cgctggatat tttcttgcgc cagggtctgt
     4321 tcaatttgga taaatccgcc acgcatgatc ccgcgagagg ctccgtagag cactgcaacc
     4381 agccccaggc aggtgaggga aatgatcagt attgtttttc gtctgaccgt catctgagtt
     4441 actttcctcg gtgcaccggc tatggctcaa attccagaca attgccaatg aatatggagt
     4501 gcatcttcat gcccatcctg cgtatcggca cgtttatcga aagtactgag ttaattcggt
     4561 ttggggaacg gagaattagg aattcttaca acggaccatg agcagcaatc caaaaaggaa
     4621 gacttgtgcc tgaacggaac attacacatt tcacatctgc tcgcggcctg gacttacccg
     4681 cacggctggc cgcagaggct gcatcgacga gaaattacgc actacgttcg gaatgacgag
     4741 ccatcgtcga gtagcgcggc cgctcttggt tgtgtgcatt ttgcaaaacc cgcacaggca
     4801 agagtgcccg tgctacgcaa acatccgccc gaacgatttt tccgcggctt gtttatccgc
     4861 tgaaagcatg accttggaat ttccacacag attttccagc cactccgaag ccaactactg
     4921 cgggacggcg tatttcgagt cgaccggcga atacaacgtg gcgccggggt cgtgcgactc
     4981 ctcaaacgca gaaatatcaa tctcgtgctt cagcgtcagc ccaatatcgt cgagcccttc
     5041 gagcagaaga tgtttccgga attcggcaat ttcaaactta taaagcaaac cgctcgcgtc
     5101 gctaatggtt tgcgctggca gatccacggt cagtgaataa ccctcctgcg attccgcgcg
     5161 gcggaacaat tcgtcaattt gcgcttccgg gaggacgatc ggcagaatcc cattcttgaa
     5221 acaattgctg taaaaaatat ccgcgaagct cggagcaatc aagacgcgaa agccgtaatc
     5281 ggcgatcgcc cacggcgcgt gttcgcggct cgaccctgaa ccgaatccag cgcgagcgag
     5341 caggattgaa actcccttat accgcggcca gttcatgatg aaatctggat tcagcgtttc
     5401 gtcaggcaag tagcgccagt cgtaaaacaa aaccttgccg taaccgtcct tggtctgcag
     5461 cttcaaaaat tgcttgggca ccatctggtc ggtgtccacg ttgacgcgat caaacggcag
     5521 aacaacccca gtgtgtttag aaaatgtctg cataagtctc cgtttttatg tggcacaggc
     5581 tttttaccct gatcgctccg aagggcagca tgcggaattt tgtccttgaa aggactaagt
     5641 tccacaggct aaagcctatg ccacaaaata cctattccgt ggcctcaaca tcccaatccc
     5701 gcacatctac aaaatgtccg gcaatcgccg ccgcggcagc catgatcgga ctcaccagat
     5761 gcgtccggct gtcttttccc tggcgtcctt cgaagttgcg gttcgaagtg ctcgcggcac
     5821 gctctccggg cagcagcaca tccggattca tcgcgatgca catgctgcat cccgcgtcac
     5881 gccattcgaa acccgcgtcg cggaaaatct tgtccagtcc ctcagcctct gccttttctt
     5941 taatcgtcat ggagccgggg accacgagcg ctcccttcaa gtttttcgcc acgtgctttc
     6001 ccgcaaccac gcgagccgcc gcgcgcaaat cgttaatccg cgaattagta caagagccaa
     6061 tgaacacgcg atctacgggc acgtcaacta acgcggttcc cggcgttaag cccatatagg
     6121 tgagcgcgcg ttcgacagcc ttccgatcgt cgggatcgcg gaatgcgccg gggtccggca
     6181 cgcgatccgt gactcctgcg accatgcccg gattcgttcc ccacgtgact tgcggcgcga
     6241 gcgcggacgc atcgagttct accgtcgtgt caaacgtcgc acccggatcg ctggccagag
     6301 ctttccaatt ctgcacggcg tcctgaaatt cctttccgcg cggcacgtat ggccgcccct
     6361 ccatgtaggc aaacgtcgtg tcgtccgggg caatcattcc tgctcgcgcg ccaccttcaa
     6421 tcgacatatt gcagacggtc atgcggccgt ccatggacag ggcgcgaatg gcttcgcccg
     6481 cgtactcaaa aacgtgtccc gcaccgccgc caattccaat tttgccgatt atggccagaa
     6541 taatgtcctt ggccgaaaca ccctgcgtcc gcttgccatt gaccacgatg ttcatggttt
     6601 tcgattcgta ttgctgcatg gtctgggtag ccagcacgtg ctccacctcg ctcgtgccga
     6661 tgccaaacgc cagcgtgcca aatgcgccat gcgtcgacgt gtggctgtct ccgcacacga
     6721 gagtttgtcc gggctgcgtg atacccaact ctggaccaat cacatgcacg attccctggt
     6781 tgtggctgaa catgtcaaaa agggtgatgc cgttctcgcg gcaactgcgc tcaagcgcgt
     6841 caaactgccc gccggcggcc ttgtccagga tcggcaggtt gcggtctttc gtcggcaccg
     6901 agtgatccat caccgcaaac gtaaggtccg gtcggcgtac tttgcggccc gcagccttca
     6961 gtccggcgaa ggcctgcggc gaggtcactt catgaatgag gtggcggtca atataaatga
     7021 gcgaaggttc gccctcggct tcgcgcacca ggtgagactc ccaaatcttg cggtacattg
     7081 ttctcggatt catatttgtg catcctaatt tagcaggcct tcgcaaactg tgcggacttt
     7141 ttcgatcgcg gcgcacgcgc ggaatgaacg tacggctgat tctctgaacg cgcgctgcgc
     7201 ttcgtttaat ttcggcgacg cggatattgg aaccggaatt gatgctttac gcaaatttat
     7261 aatggttggg tcatctataa agtttag
//
LOCUS       DASZMB010000020        11451 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4411704, whole genome shotgun sequence.
ACCESSION   DASZMB010000020 DASZMB010000000
VERSION     DASZMB010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 11451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11451
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4411704"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /gene="alr"
                     /locus_tag="VG272_00730"
     CDS             complement(<1..1059)
                     /gene="alr"
                     /locus_tag="VG272_00730"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWF12233.1"
                     /translation="MTRTVKFDGRPIWAEVSLGALTHNLRAIRKHVNPEGARGPRRKI
                     LAVIKSNAYGHGIVPVARALSKVRADWFGVTCSAEGAELRDSGIREPVLILTGFWAGE
                     EQRILDHHLTPTIMGADQLRLLEKAAARQRKPRKKPVDFHLKIDSGMNRLGIPPIEIP
                     AFARVLADCPHLRLGGTFTHIASSEVFTTEQTVEQEKVFDSALGCMRSLGLDPGIVHM
                     ANSAAIVARPSTWRDMVRPGAILYGYHQFFEPSELNAEMEAKLPVRPVLSFRARIISI
                     KDLPAGARVGYNARWTAVKSSRVAVLAAGYADGFVRSLSNKGRVIVRGEIAPIIGTVS
                     MDLTTADVTGISSARVGDI"
     gene            complement(1410..2774)
                     /gene="dnaB"
                     /locus_tag="VG272_00735"
     CDS             complement(1410..2774)
                     /gene="dnaB"
                     /locus_tag="VG272_00735"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HWF12234.1"
                     /translation="MATASTSLERPMPHNVEAERSILGAILLDNNAINAAVERLKPED
                     FFHDHHRRIYQQMITLGEAQQAIDLVTLTEQLQRSGELESVGGAAYVSQLMDGVPHIT
                     NVEHYARIVKEKSLLRGLIHATSAIQQQALEAEEDADAILDRAESSIFQLAEDRVRMG
                     LVGLHEVVTESHERISRIYEGGHRITGLSTGYDMLDNLTSGLQPSELIILAARPSMGK
                     TAFALNIAENVGVRGNKPVAIFSLEMSKESLLLRLLSADAKIDSHKFRTGHLNADDKA
                     RIPRSLAHLSQAPLWIDDGGAATVVEMGAKLRRMKRDKGLSLVIVDYLQLVSARGRFG
                     NRNEEISSISHGLKAMAKELKVPMLVLSQLTRAPEREDREPRLADLRESGAIEQDADV
                     VMFIHRPNFFKKSSEVSMEERGETDLIIAKQRNGPTDSIRFIFQGGFTRFEQRAPDAW
                     TEQE"
     gene            complement(2827..3105)
                     /locus_tag="VG272_00740"
     CDS             complement(2827..3105)
                     /locus_tag="VG272_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12235.1"
                     /translation="MTNDLHGPVAILDKEIAKYPTYTIETFELCDCCGARFGIGYHQG
                     CKHHPAAAGQENELPKKLREMLAADHGHDRKHKHFTEIWVLSDWGASA"
     gene            complement(3427..4197)
                     /locus_tag="VG272_00745"
     CDS             complement(3427..4197)
                     /locus_tag="VG272_00745"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HWF12236.1"
                     /translation="MATPMPQQNDSRPRIGVPWRMAAEEAANKRTKIDNYLQAVEKAG
                     GKAVLLSLALNKDDLKRQAAVLDGFVLTGSPADLEPTHYGATRHPATDAADAARERTD
                     FTLLEHALIAAKPVLAICYGIQSLNVFLGGSLIQDIPTEIGTKIIHSPEDDELPGGGE
                     APDAMHFVAIDPGHLRDLSKAPSAEVNSWHHQSILEPAHGLRVTARASDRVVEAVEWT
                     DNSNWVVGVQWHPERMPRDPLACALFSQLITNARLIRA"
     gene            complement(4377..6248)
                     /locus_tag="VG272_00750"
     CDS             complement(4377..6248)
                     /locus_tag="VG272_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HWF12237.1"
                     /translation="MSSNRRAVLIGLIVILGLTGSGIVLTTEWGARTVSSTRASQQVS
                     QSPVDLSQFRNTQSLAELAATPQEQDLARDALRKADHEVDFEFTSALYRASSKKAAST
                     PEIKAILDRISKDEQDNAQLDAEVERLTKLAASAKDAQKEGITQQLDLTKSRQELVED
                     EIDDAHQDLERAGGDPQKQVQRMVDEYHASEQSSGGQLDLSIVGKKSGSTQPASASFI
                     SRAQAWYALRPVVGGLDQAQQQASNSIATFSKSHDDLEQQLNQRKLQQSQKQDAAPQT
                     NAPGNAAATAPSNASSQTSEAISSYKSMTILQKRMSGLDSRIRNQQDLASIYSQWHDL
                     VAARHRELLHSLLISVALMLAIALAVVLTTQSLEKLFARVTSDPQRLVTMRSVANIVA
                     RAIGAILILLIIFGMPSQLATVIALATAGLTVAMKDFIVGFFGWFVLMGKNGIRHGDW
                     VEINGVSGEVVEIGLFHTVVLETGNWNDAGHPTGRRVTFMNSYAIEGHYFNFSTSGQW
                     LWDEMQLALPLDRDPYPVATEIQKIVNSETEANARLAEKEWGTMGSARGIRPFSAGPA
                     ISVRPANAGFEIIIRYVTRANERHQQRAKLYQDIVELLRRKNIPEPTPRAASAPAAS"
     gene            complement(6314..7378)
                     /locus_tag="VG272_00755"
     CDS             complement(6314..7378)
                     /locus_tag="VG272_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWF12238.1"
                     /translation="MQIEDAKVTPRSNPSQRIIAVAIVFAALYFTSAVVVPLLLAVLL
                     AYFLDPVVELLERIRVPRPLGAIIVLLAVTAIFAGLGYLIVLRADQFVADWPRYSSVL
                     RHMATAFDRQLAVVQKRVDAITPIADKALTPAHLVEPPPIRDWLLSGVGSLYSILVVA
                     VFVPFLVFFMLAAKPRIWKATIELFPHGHQKRVRDALDQLSSMLRSFVAGNALIAAIL
                     MLLSYGFFLTIHLKYPFLSGCVSGILNLVPYLGAVLAWLPPFLIGMAQWNTIGPYVGV
                     AAMLTFLHIMGLNVLMPAIVGRRVHLNALAVTIALLFWGWLWGAVGLILALPITATVK
                     VICDHSEGWEPVGRWLGEEN"
     gene            7481..8311
                     /gene="cdaA"
                     /locus_tag="VG272_00760"
     CDS             7481..8311
                     /gene="cdaA"
                     /locus_tag="VG272_00760"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HWF12239.1"
                     /translation="MIAAALPVTSLSRLDLRAAVDIVIIAVFIYYLLKLLRGTRAVQM
                     LVGIALLFAFYRGAQWARLEMVEWLLTTMLPYIAIALIILFQPEIRRALSRVGRNLSL
                     ISFASRNPKGTYDDIVMAAEYFSQNRIGALIVVERQAGLRTYIESGIPLDAKLSYDLL
                     LSIFRPGSPLHDGAVIIEGSRVAAAGCFLPLSLNPMISKQLGTRHRAAIGITEDSDCL
                     VVLVSEETGSISVASSGSIETNLSPEDLSDRLREMVSRRRTTMVLPAAVPQARTPVRR
                     "
     gene            8315..8722
                     /locus_tag="VG272_00765"
     CDS             8315..8722
                     /locus_tag="VG272_00765"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HWF12240.1"
                     /translation="MEFLRKYVFANAALKLLALAISFTLWITYTSEPYAEVGFQVPVE
                     FTTMPTQLEITGDIPTAVRVRVRGRSALLRRMISPDLSLRLDMKDGKPGTTNLQITPE
                     MVIAPYGATVVQVTPAEIHVTLVPRHSLAPPTG"
     gene            8820..10163
                     /gene="glmM"
                     /locus_tag="VG272_00770"
     CDS             8820..10163
                     /gene="glmM"
                     /locus_tag="VG272_00770"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HWF12241.1"
                     /translation="MTKELFGTDGIRGIPGTYPLDDATLYGVARALGKYLLEVDPKPR
                     VLIGMDTRESGPHIAGQLAAGLSDNGVEFLSAGVITTPGIACLVRQKKFSAGMVISAS
                     HNPFHDNGVKLFAGTGMKFPDDVEEHLETEIHQHKGRETSPHGIPVPTDLSLDEEYLA
                     YLRKCIIPGANLKGLKLVLDCANGAASMLGPTLFRSLGAEVIVIHNQPNGRNINDGCG
                     SLHPEQLREKVLETKSALGAAFDGDADRAMFVSSSGRHVDGDGVLLAAARYFKSKDQL
                     CGSRVIGTTMANLGLERILEAEGLTLARVPVGDRYVLEEMLRSGANLGGEQSGHIIFL
                     DDATTGDGLLTALKMACLVALRGPLDELVSGFKVFPQTIVNVKVKSKPPLESLPVVSA
                     AIEKATKALGAAGRVVVRYSGTEPKARVMVEAEHLEDVSLWSEALASAIRAAIGA"
     gene            10619..11224
                     /locus_tag="VG272_00775"
     CDS             10619..11224
                     /locus_tag="VG272_00775"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HWF12242.1"
                     /translation="MARSVDEDLAQLERDIRQLKIEYEQYFGGGKARPPADIEWRIDT
                     CLKRYSDRSAGMNYGQRFRYGNLTQMYTKFRDIFRKRMKQKEEGTVQRHFGAAAREIE
                     AQRALKRAQQDKKITEQFPFAVSWSDPDTEAKKVEQLFAAFGHAKAKTGEDAKALTME
                     VFQRFVRQKTAQLKKQQNAHEVEYVVTIEGNQARLKARVKS"
BASE COUNT         2622 a         3082 c         3104 g         2643 t
ORIGIN      
        1 gatgtctcca acgcgagcgg aactgatacc cgtaacgtca gccgttgtaa ggtccatcga
       61 aactgttccg ataatcgggg caatttctcc gcgaacaatc acacggcctt tattggataa
      121 agagcgcaca aaaccatccg cgtatcccgc ggcgagcacg gcgactcgtg aagacttcac
      181 agcggtccat cgcgcgttgt atccgacgcg cgcgcccgcg ggcaaatcct tgatcgaaat
      241 aatgcgcgct cggaaagaca aaacaggacg gacaggaagt ttcgcttcca tttctgcatt
      301 caactcggac ggctcaaaaa attggtgata gccgtaaaga atggcgccgg gacgaaccat
      361 gtcccgccag gtagatggcc gcgcaacgat cgccgcgcta ttggccatgt gcacgatgcc
      421 tggatcgagg ccaagcgaac gcatgcagcc tagcgccgaa tcaaaaactt tttcctgctc
      481 gacggtttgt tccgttgtaa aaacttccga cgatgcaatg tgcgtgaatg ttccgccaag
      541 ccgcaaatgc gggcaatccg ccaacacgcg cgcgaatgcc ggaatttcta tgggcggaat
      601 ccccaggcga ttcattccgg aatcaatctt caaatgaaaa tcaaccggtt ttttccgcgg
      661 cttgcgttgg cgcgcggccg ctttttccag caggcgtaac tggtcggcgc ccatgatggt
      721 cggcgtaagg tggtggtcca gaatccgctg ctcttcgccg gcccagaatc cagtgagaat
      781 cagcacgggt tcgcgaattc cgctgtcgcg caattcggcg ccctcggcgg aacatgttac
      841 gccgaaccaa tccgcgcgca ccttcgatag cgcgcgggca acgggcacaa ttccgtggcc
      901 gtaggcgttg cttttaatga cggcaagaat tttccggcga ggcccgcgag cgccctcggg
      961 attgacgtgc ttgcggatcg cacgcaggtt atgcgtcagc gctcccagac ttacttccgc
     1021 ccaaatgggg cgtccatcaa attttacggt tcgtgtcatt ggagtcatca agattaaccg
     1081 atccgtgcaa agaaaaaaat ttgccgatgc gcaacagcgc accgcttagt aactagccct
     1141 ttgtggcata ggcttcagcc tgtggaattt agttcttgca aggcctaacc tccacaggct
     1201 gaagcctatg ccacacgaag caccatccac tcttccttgt gtagaggtgc aagtatcggg
     1261 ccgtgggcta ggactgcgag accccgcact ggaaagagct tatgtaatag aaaagattga
     1321 agctgtcggg aaggtagtga aaactctcag gggcattaac cgtttcaatc tgactcacac
     1381 cgcacacacg tgtgtgtgtc cgaggacgcc tactcctgct ccgtccatgc gtcgggcgct
     1441 ctctgctcga aacgcgtgaa gccgccttga aagatgaacc ggatcgaatc ggtgggaccg
     1501 tttctttgct tggcgataat caggtccgtc tcgccacgct cttccatgct gacttcgctc
     1561 gatttcttga agaaattcgg ccgatgaata aacatcacga cgtcggcgtc ctgctcaatg
     1621 gctcccgact cgcgcaagtc cgcgaggcgc ggttcgcgat cttcgcgttc cggcgcgcgc
     1681 gtaagctgcg agagaacaag catgggaact ttcagttcct tggccatggc tttcagtccg
     1741 tgcgaaatgc tggaaatttc ttcgttgcgg ttgccgaatc gtccgcgcgc ggaaaccagt
     1801 tgcaggtaat ccacgatcac gagagaaagt cctttgtcgc gcttcatgcg gcgcagcttc
     1861 gcgcccattt ccactacggt tgcagcgccg ccatcgtcga tccaaagagg agcttgcgaa
     1921 aggtgggcca gtgagcgtgg aattctggcc ttatcgtctg cattcaagtg gccagtccga
     1981 aatttgtgcg aatcgatttt tgcgtcagcc gaaagcaagc gcagcagcaa cgattccttg
     2041 gacatttcca ggctgaaaat cgccaccggt ttatttccgc gcacgcccac attctcagca
     2101 atgttcaggg caaaagcggt tttccccatc gaaggccgcg ccgcgagaat aattaattcg
     2161 gaaggttgaa ggcccgaagt caaattgtct agcatgtcgt agccggtcga gaggccggtg
     2221 atgcggtggc caccttcgta aattcgggag atgcgttcat ggctctcggt gacaacttca
     2281 tgcaatccca cgaggcccat gcgcacgcgg tcttccgcaa gttgaaagat ggaagattca
     2341 gcgcggtcca ggatggcatc ggcatcttct tcagcttcta atgcttgctg ctgaatcgcg
     2401 ctcgtagcgt ggatcaggcc gcgcagcagt gatttttcct tcacgatgcg cgcatagtgc
     2461 tcgacgttcg tgatgtgcgg cacgccgtcc atcaattgcg aaacgtaggc cgcgccgccg
     2521 acggattcca gttcgccgct acgctgtaac tgctcggtca gcgtcacgag gtcaatggcc
     2581 tgttgcgctt cgcccagcgt aatcatctgc tgataaatgc gccggtgatg atcgtggaaa
     2641 aagtcttcgg gcttcagccg ctcgacggca gcgttgatgg cattgttgtc gagtaggatc
     2701 gcgccaagaa tgctgcgctc ggcctcgaca ttgtgaggca tgggacgttc aagagatgtg
     2761 ctcgcggtcg ccatttttat tcgccttata ttcgcctaaa tatcggcttc cggcaagcta
     2821 aaccaatcag gcagaggcgc cccaatcgga aaggacccag atttcagtga agtgcttatg
     2881 cttacgatca tgcccgtggt ccgcggcgag catttcacgg agctttttgg ggagctcgtt
     2941 ctcctggccc gcggctgccg ggtgatgctt gcatccctgg tgatacccaa tgccaaatcg
     3001 ggcgccgcag cagtcgcaga gctcaaaggt ttcgatcgta taggtcggat actttgcaat
     3061 ttccttgtcc aagatcgcta cgggaccatg caaatcattc gtcatgtcat cctccgctgg
     3121 tttgaggatt ccctggctca actggctgtc attcttcgcg tcggactctc agtggagtcc
     3181 aagtttgtgg cgccgcacat cgcggggcag aaccaaaatc aagagtgatc gaccgggaaa
     3241 gtctatcttg aaaagtaaaa tcgcgccctg tggaattcgt gtgaacctgt gcataacttt
     3301 tcgaggagtt ttggggaata ttttgcgaaa aatttcaaga acgcattcga aattcgaagt
     3361 tggcactcgg agggaatgcc agctgaaggc gggcgccaca cgggcggcta actaaaaaat
     3421 ttatttttat gcgcgtataa gccttgcgtt tgttattagc tggctgaaaa gagcgcaggc
     3481 caagggatcc cttggcattc gttccgggtg ccattgcacg cctaccaccc agttggagtt
     3541 gtctgtccac tcgacggctt cgaccacgcg atcggacgcg cgagcggtga cgcggagccc
     3601 gtgcgctggc tccaggatgg attgatgatg ccacgagttc acttctgcgc ttggagcctt
     3661 ggacagatcg cgcaagtgcc cgggatcgat ggcaacgaag tgcatggcgt cgggagcttc
     3721 gccgcctccg ggcagttcat catcttccgg gctatggata attttcgtgc caatttcggt
     3781 gggaatgtcc tggatcaggc tgccgcccaa gaaaacgttc agcgattgaa tgccgtagca
     3841 aatggccagc accggcttcg cagcgatcag tgcgtgttcc agcaaagtga aatcggtgcg
     3901 ttcgcgcgcg gcatcggctg cgtcagtcgc aggatggcgt gtcgctccat agtgcgtagg
     3961 ttccagatcg gcggggctgc ccgtgagcac aaagccgtcg agaacagcgg cctggcgctt
     4021 taaatcatct ttgtttaagg caagagacag cagcacagct ttgccgcccg ctttttccac
     4081 agcctggaga taattgtcaa ttttggttcg cttgttggcc gcttcctcgg ccgccatgcg
     4141 ccacggtaca ccaatccggg gccgggaatc gttttgctgg ggcataggag tagccatcct
     4201 agcatcgggc cgccatgcca gcaatatggg cgcatcagct gtggagccgg cgtctcgccg
     4261 atgctttttt gccctatgac gcaccgcgca aaaccgccgg cgagacaccg gctcgacaaa
     4321 aaattctctg gggaaaatta tggatttgat tttaaagaat tgcccgaagc attttgctaa
     4381 ctcgcagctg gcgcggaagc cgctctggga gttggttccg gaatattttt tcgccgcaac
     4441 aattcgacga tatcctgata gagcttcgcg cgctgctggt gccgttcatt ggcacgagtc
     4501 acatagcgaa ttatgatttc aaatcccgca tttgccgggc gaacgctgat cgcggggccc
     4561 gcggaaaacg gtcgaatgcc gcgagcactt cccatggttc cccattcttt ttcggcaagc
     4621 cgcgcattcg cttccgtctc gctgtttacg attttctgaa tttcagtggc gacaggataa
     4681 gggtcgcgat ccaaaggaag tgccagctgc atttcatccc acagccactg gccggacgtt
     4741 gaaaaattga aatagtgtcc ctcgatcgcg tagctgttca tgaacgtgac gcggcgccct
     4801 gtgggatgtc ccgcgtcatt ccagttgccg gtttccagca ctacagtgtg gaacaatcca
     4861 atttcaacga cctcgccgct cactccatta atttcgaccc aatcgccgtg gcgaattcca
     4921 ttttttccca tcagcacaaa ccagccgaaa aatccgacga taaaatcttt catcgccacg
     4981 gtaaggcccg cggtcgccag tgcaatgaca gtcgccagct ggctgggcat tccaaaaata
     5041 atcaacaaaa tcagaatggc gccaatcgcg cgcgccacga tgttcgcaac cgaacgcatg
     5101 gtcacaagtc tttgcgggtc ggaagtcact cgagcaaaca atttttccag cgattgagtg
     5161 gtgagcacga cagcaagcgc aatagcaagc atcagcgcaa cggaaataag caggctatga
     5221 agtaattcgc ggtggcgagc agcaactaaa tcgtgccact ggctgtagat cgatgccaga
     5281 tcctgctggt tccgaatgcg gctatcgagg ccggacattc ttttctgcaa gatggtcatc
     5341 gatttatacg atgaaattgc ctcgctggtt tggctggagg cgttagaagg agcagtcgct
     5401 gcggcgttgc cgggggcatt tgtctgtggc gcggcgtcct gtttttgaga ctgctgcaat
     5461 ttcctctgat tcaattgttg ttcgaggtcg tcgtgcgatt tggaaaacgt cgcgatggaa
     5521 ttcgaggctt gttgttgcgc ctggtcgaga ccgccgacta caggtcgaag agcgtaccaa
     5581 gcttgcgcgc gcgaaataaa agaggcagaa gccggttggg tcgagcccga tttcttgcca
     5641 acgatgctta gatcaagctg gcccccgctg ctttgctcgg aagcgtgata ttcgtccacc
     5701 atgcgctgca cttgtttctg aggatcaccg ccggcacgct cgaggtcctg atgcgcatcg
     5761 tcaatttcat cttccaccaa ttcctggcgg ctcttggtta aatcaagttg ctgagtaatg
     5821 ccttctttct gagcgtcctt cgcacttgct gcgagcttgg taaggcgctc aacctccgca
     5881 tccaattgcg cattgtcctg ttcgtctttt gaaatccggt caagaatggc ctttatttcg
     5941 ggcgtagatg cggccttttt ggaagaggcc ctgtacagcg ccgaagtaaa ttcaaaatcc
     6001 acttcgtggt ccgccttgcg cagggcgtcg cgcgccagat cctgctcctg gggcgtcgcc
     6061 gccagctctg caagcgattg cgtattgcga aattgcgaaa gatcgactgg agattgcgaa
     6121 acttgttgcg acgcgcgtgt ggacgaaact gtacgcgcgc cccactccgt agtcagcacg
     6181 atgccgcttc cggtcagccc aagaattaca attaaaccaa ttaaaactgc acgccggtta
     6241 gatgacataa tttgttccga tttccttgcg tcggcgctcc caccggttgg gcgggggaag
     6301 cggcgtcacg aattcaattt tcttcgccga gccagcggcc caccggctcc catccttctg
     6361 aatgatcgca gattaccttc accgttgccg tgatcgggag tgccaggatg agtcccacgg
     6421 ctccccacaa ccaaccccag aacaataagg cgattgtcac agccagggca ttcaaatgga
     6481 cgcgcctgcc gacaatggca ggcatcaata cgttcaggcc catgatatgc agaaacgtaa
     6541 gcatggctgc aaccccgacg tacggcccaa tcgtattcca ttgcgccatg ccgattagga
     6601 atggagggag ccatgcaagt actgctccta aatatggcac taaatttaaa attcccgaga
     6661 cgcaacctga taagaacggg tatttcaaat ggattgtgag gaaaaatcca taactcaata
     6721 gcatgaggat agcggcaatc agcgcgttcc ctgcgacaaa gctccgcagc atcgaactga
     6781 gttgatcaag tgcgtcccga acgcgtttct gatgcccatg tggaaataat tcaatggtag
     6841 ctttccaaat gcggggtttt gctgccagca taaagaaaac gagaaacgga acgaagaccg
     6901 ccacaaccaa gattgagtag agcgatccca cgccgctaag aagccagtca cgaatcggtg
     6961 gcggctcaac gaggtgcgca ggagtgaggg ccttatccgc aattggcgta atggcgtcga
     7021 cccttttttg cacgaccgcg agttgccggt cgaaggccgt agccatgtgg cgcaggacgc
     7081 tgctatagcg cggccagtcg gcaacgaatt gatccgctcg aagcacaatg aggtagccca
     7141 ggcccgcaaa aatcgcagta acagctagaa gcacaatgat tgcgcccagc gggcgcggaa
     7201 cgcggatacg ttcgaggagc tccactacgg gatcgagaaa ataggccagc aaaacggcga
     7261 gcagcaacgg aaccacaacc gcagaggtga aatacaacgc ggcaaacaca atcgccacag
     7321 caataatgcg ctgagaaggg ttcgatcgcg gcgtaacttt ggcatcttcg atctgcatat
     7381 tccaccaaat tcaccttagc atatcgatac attgcggcaa atcccgcgaa acaacggctt
     7441 gccccaagca gcaaggctga gataacttag atgcacgatc atgatcgctg ccgctttgcc
     7501 cgtgacctct ctgtcgcgcc tggatttgcg cgccgctgtg gacattgtaa tcattgcagt
     7561 ttttatctat tacctgttga aacttttgcg cggcactcga gctgtgcaaa tgttggtggg
     7621 aatcgcgctg cttttcgcct tctaccgagg cgcacagtgg gcaaggctgg aaatggtcga
     7681 atggctgctg acgaccatgc tgccgtatat cgccatcgca cttattattt tattccagcc
     7741 ggaaattcgc agagcgctgt cccgggtggg ccggaatctt tccttgatca gttttgcttc
     7801 gcgcaacccg aaaggcacgt acgacgatat tgtgatggct gccgaatatt tttctcagaa
     7861 ccgaatcgga gccctgattg ttgtggaacg gcaagcaggg ctgcgcactt acattgaaag
     7921 cgggattccg ctggacgcca aactttctta tgatttgctg ctttccattt ttcggccagg
     7981 ttctcccttg catgacggtg ctgtgattat cgaaggttcg cgcgtggctg ccgccggatg
     8041 cttcctcccg ctttcgctca atccgatgat ttcaaaacag ctgggcactc ggcatcgcgc
     8101 ggcgatcgga atcaccgaag atagcgattg cctggtcgtg ctggtttccg aagaaacggg
     8161 atctatctcc gttgcttcct ccggatcgat cgaaaccaat ctttcaccgg aagatttgag
     8221 tgaccggctg cgcgaaatgg tttcgcgccg ccgcacaacc atggtcctgc ctgccgcggt
     8281 tccgcaggca agaactcctg ttcggagata atttttggaa ttcttgcgca aatacgtttt
     8341 cgccaacgca gcgctgaagc tgctggcgct cgccatttcg ttcactctgt ggatcaccta
     8401 cacctccgag ccgtatgcgg aagtcggctt tcaagtgcca gtggaattca ccaccatgcc
     8461 cacgcagttg gaaatcacgg gagatattcc gacggctgtg cgagtccgcg tgcgggggcg
     8521 atccgcactg ttgcgacgca tgatcagtcc cgacctgagt ctgcgcctgg acatgaagga
     8581 cggcaaaccc ggaaccacga atttgcaaat cacgccggaa atggtgattg ccccctatgg
     8641 cgctaccgtc gtgcaggtta cgcctgcgga aatccacgtt actcttgtgc cacgccattc
     8701 acttgctccg ccaactggat aaccttacgc gtgctaaaat tagaaatccg agagtgaaca
     8761 aagtgcccgg acaaatttga ttcctgcaac tccgagaaga ggctgcagct ccatccacga
     8821 tgactaagga actgttcgga accgacggca ttcgagggat tcctggcact tatccgttgg
     8881 acgatgccac gctctatgga gtcgcccgtg cactgggaaa atatttgctg gaggttgatc
     8941 ccaagccgcg cgtcctgatt ggcatggata cgcgcgaatc cggcccccac atcgcggggc
     9001 aactggctgc gggcctttcc gataacggag tggagttcct ttccgcaggc gtaatcacta
     9061 caccgggcat cgcctgcctt gttcgtcaaa agaaattctc cgccggtatg gtcatatcgg
     9121 cttcacataa cccctttcat gacaatggcg taaagctctt cgccggtaca gggatgaaat
     9181 tccctgatga tgttgaagag catctggaaa ctgagattca tcagcataag ggtcgcgaaa
     9241 cctctcccca cggcattcct gtcccaaccg atctttcgct ggatgaagag tatctggcct
     9301 atttgcgcaa gtgcataatc cccggagcga atctcaaagg gttgaaactg gttctcgatt
     9361 gtgccaatgg tgccgccagc atgttgggac caactttgtt tcgctcgctg ggcgctgaag
     9421 tcattgtcat tcacaatcag ccgaatggcc gcaacatcaa tgacggttgc ggttcgctgc
     9481 atccggaaca gttgcgcgaa aaagtcctgg agacaaagtc agcgctgggc gcggcgtttg
     9541 atggcgatgc ggatcgcgcc atgttcgtaa gttcgagcgg gcgtcatgtg gatggcgatg
     9601 gtgtcttgct tgctgctgcc agatatttca aatcgaaaga tcaactttgc ggcagccgtg
     9661 taattggcac tacgatggcc aacctggggc ttgagcgcat cctggaggca gagggactca
     9721 ctttagcccg tgttcctgtt ggagaccgat atgtgctgga ggaaatgctt cgcagcggcg
     9781 cgaacctcgg cggcgagcag tctggccata ttatttttct agatgatgca acgaccggcg
     9841 atggcctttt gaccgcactc aagatggctt gcctcgtggc tcttcgcgga ccgctggatg
     9901 agcttgtcag tggtttcaaa gtgtttccac aaactattgt gaatgtgaag gtcaagtcta
     9961 aaccaccctt ggaatccctt ccggttgtct cagcagccat tgagaaagcc acaaaagcgc
    10021 tgggagccgc agggcgcgta gttgtccgct attcagggac agaacccaaa gcccgcgtga
    10081 tggttgaagc tgagcacttg gaagacgtct cgctatggtc ggaggctttg gcctcggcta
    10141 ttcgagctgc aatcggtgcc taatatccca aagacagtag ctacccagcc ccaatatgtg
    10201 aaaaactgta gattttctgc agcgaaccac tacgcgacat gaagccttgt tgtcctatca
    10261 tactgattct acacaaaata caccattact taatttttat taacccttgg catccagttt
    10321 gcacttaggt aggtcaagtg gttttgattt tcccggggtg gttggttgat atgttccggc
    10381 ttcgttccgg aacggtgaat ggggtattgc tggaaggggg gttcggcaac gagccccctt
    10441 tcattttttg ggccagttcc gcaaggctta agccatctgt tttcaactct cacattttca
    10501 actctctcat aaggctcggc catattcgtc attatctgtt ccgcacttca tgtcatgtag
    10561 cgcgttgacg cttttggcta ccaccaagta gcatcgttac ttatgtgggt tacatggaat
    10621 ggctagatcg gtcgatgagg atctcgccca gcttgaaaga gatattcgtc aacttaagat
    10681 cgagtatgaa caatacttcg gtggcggcaa agctcgtcct cctgcggata ttgaatggcg
    10741 catagacaca tgcctcaagc gctatagcga ccggagcgcg ggaatgaatt atggccagcg
    10801 ctttcgctat ggcaacctca cccagatgta cacgaaattc cgcgacatat tccgtaagcg
    10861 catgaaacag aaagaagaag gaactgtcca acgccatttc ggcgcggcag cccgcgaaat
    10921 tgaagcgcag cgcgcactca agagagctca acaggataaa aaaataaccg agcaatttcc
    10981 gttcgcagta tcctggagcg atcccgatac ggaagccaaa aaagtggaac agctttttgc
    11041 cgcatttgga catgccaaag caaaaacagg cgaggatgcc aaggctttga ccatggaagt
    11101 tttccagaga tttgtgcgac agaaaacggc ccagctcaag aagcaacaaa acgcccatga
    11161 agtggaatac gtcgttacga ttgaaggcaa tcaagcacgg ctcaaagccc gcgttaaatc
    11221 ctgaaaattc cctctccgaa tgctcccctt aaatttaggt gcagccttca gagcttgcgt
    11281 gagaactgca aaattgaagc ttcagcggct aaagagtttg cggaaaaagt agtttattct
    11341 gtcattccga gcgaagcgag gaatctctct tcgatttaag tataagaaaa taaagaagga
    11401 gggattcctc gcttcgctcg gaatgacaaa atgttgagtt tttccgcagc c
//
LOCUS       DASZMB010000021        11345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1113838, whole genome shotgun sequence.
ACCESSION   DASZMB010000021 DASZMB010000000
VERSION     DASZMB010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 11345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11345
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113838"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VG272_00780"
     CDS             complement(<1..649)
                     /locus_tag="VG272_00780"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWF12243.1"
                     /translation="MGCRAFGVRGDFRAVLFSLGLIALAVFPSAVHAQVSGSISGNVQ
                     DSSGGVLRDAKITVKSQESGATRTAVTDDSGSYRVLSLPLGPQELKAEKQGFKSVVRT
                     GVSLEVGQEAVVNFRLEVGELVQQMSVSEEAPVVNVTTASISGVVDERQIKELPLNGR
                     SFDDLIALNPGTINYSALKSPNTSTSNGNTFSVAGRRTSENLFLLNGVEYMGSSQL"
     gene            complement(697..1836)
                     /gene="bshA"
                     /locus_tag="VG272_00785"
     CDS             complement(697..1836)
                     /gene="bshA"
                     /locus_tag="VG272_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HWF12244.1"
                     /translation="MKIGITCYPTYGGSGVVATELGMELAARGHEIHFISYAPPIRLT
                     DTNDRIQFHEVEISSYPLFDHAPYALSLAVKMMQVAEAESLDLLHVHYAIPHSVSALL
                     ARMMAAPKRLPFITTLHGTDITLVGNDRSFLPITKFSIEQSDGVTAISQYLKQRTIEE
                     FDIKRPIEVIPNFVNCDLYCRKPNQALRSEWAPDGEPILMHLSNFRPVKRITDVIEMF
                     AIVRQKMPAKLVMIGDGPDRGAAEHLIRKKKLTRDVHFLGKQDHVYQFLGQADLFLLP
                     SDMESFGLAALEAMACEVPVVASNVGGLPEVVEHGVDGYLFTPREVEVGARYALEILS
                     QEDRGRAMGKIARVNAKKKYCANDVIPLYESYYRQVIEAVTATHA"
     gene            2291..2725
                     /locus_tag="VG272_00790"
     CDS             2291..2725
                     /locus_tag="VG272_00790"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWF12245.1"
                     /translation="MKPRGKLSKADLARADERLRHSAPINHFGFQLFKAERGRAVFKM
                     PVLDLHKQIHRVVHGGVLAMLADTAGGFAAFLASPPGSRVVTIEMKINFLEAVEHGEI
                     KADARVLRQGRTTSVVDCDVTDHDGRLVSKALMTFSVSMQKM"
     gene            complement(2866..3213)
                     /locus_tag="VG272_00795"
     CDS             complement(2866..3213)
                     /locus_tag="VG272_00795"
                     /inference="COORDINATES: protein motif:HMM:NF028518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HWF12246.1"
                     /translation="MNRKLFRPTLTEMKEPHPSSTRTHSSTNGPQPHKKPAPPEQTHA
                     ENFYWIKQMQARTPVVIVMTDGENLQGIVEWYDKDCIKLTRTGSPNLLIYKQFIKYIY
                     KDGAEAALGGTNG"
     gene            complement(3307..4437)
                     /locus_tag="VG272_00800"
     CDS             complement(3307..4437)
                     /locus_tag="VG272_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12247.1"
                     /translation="MGFSEFIGNSRVVTALRGMLATDRVAHAMLFSGPRGVGKFTLAR
                     MFAQAANCERLRDDFCGECEHCRKIALLADTSSLISQGLAQRGERPDSATVERVPLLL
                     ETHPDVWAIVPDPIRSKDPVARPMLHVGQLRAVQRAAYFKPRGRRRVFIIDGAETMRW
                     DLSNIFLKILEEPPESSTLILLAPSPYQLLPTIRSRCLQFSFSPLGTAEVEKILSTHT
                     KMTPEQRKIAAQYAEGCPGTAIGMDLDAVAKLHRDALTILERSIELRGVSDLFASTSA
                     LAKSQEIAFENVLEVFYSLLTDLLDLSSSSTMKVLRNPALRRELETLSQKIDVAWINK
                     AVEGFDVLHARTRRNVNRSLGLDALAISLTRPSPGIKKSISR"
     gene            complement(4465..5154)
                     /locus_tag="VG272_00805"
     CDS             complement(4465..5154)
                     /locus_tag="VG272_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12248.1"
                     /translation="MDTRGKLIAIEGIDGSGKRTQLELLATALEERKFPTHRISFPRY
                     ESFYGKLVGLYLNGEFGTLDAVDPHLSALLYAGDRLEARPEIEAALVAGKIVLADRYI
                     GSNMAHQSARVAPEKREEFFAWLKHLEYTLYGLPAEDLVIYLRVPFEEAHRLVGLKAA
                     RDYTRLKRDIQEADIKHLEQTGIIYDRLATEANWARIDCMDASSRSLRAPEEIHQSVL
                     RCVEARILSHK"
     gene            5309..6064
                     /locus_tag="VG272_00810"
     CDS             5309..6064
                     /locus_tag="VG272_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HWF12249.1"
                     /translation="MGFASVEIEKNFADVLERIDRAASRAGRRAEEITLVAVTKTHPA
                     SKILSLYEAGARHFGENRVQERESKLAETVKVDATWHMIGHLQKNKVARVPRMFDSID
                     TIDSITLAEKLDRAAGEFLRSKNENGDLLPHPYDHRLRVLIEVKLDPELAKSGASNEE
                     LPRLVEGVLRMPHLHLQGLFGVPPYFDDPEDARPYFRHLRELRDKIREGMGAQLLPDL
                     SMGMSHDFEIAIEEGATEVRVGTSLFGQREYAE"
     gene            6061..6348
                     /locus_tag="VG272_00815"
     CDS             6061..6348
                     /locus_tag="VG272_00815"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HWF12250.1"
                     /translation="MSLEIAEKNGSVSFHVRVQPRASCDEFAGEYQNGLKIRLTAPPI
                     DNRANEALRKFLALRLNVPMTAVRIAAGEHSRTKRVEISGVSMATIRALVD"
     gene            6396..7571
                     /locus_tag="VG272_00820"
     CDS             6396..7571
                     /locus_tag="VG272_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HWF12251.1"
                     /translation="MPNIEAIQAALREQGIDGWLFYDILHRDPIAYRVLGLDHALAKR
                     RWFYMIPAKGNPRKLVHRIEAATLDSLPGEKLLYAAAGELEKNLPKLIGRAKKVAMQY
                     SPKNSIPYVSLVDAGTVELIRGQGCKVVSSAELVQQFEAAWTPEQLETHRAAGRSIDS
                     ITQAAFAEAARRVSAGENLTEYDLQQWIVAQFDAHGVVSDSPPIVAVGAHSGNPHYEP
                     MAQGSAIIRRGDFLLLDIWAKTQTPNSVYYDITWVGYLGSKIPGKYAKIFSIVREARD
                     SAVHFVQKNIAAGRNIAGWQVDGVAREVIRKAGYAKYFVHRTGHNIGQEVHGAGANMD
                     GLETRDIRRIIPHTCFSVEPGIYLSEFGVRSEVDVYVGQNTAEVTGPAQMEIVKLFS"
     gene            7587..7994
                     /locus_tag="VG272_00825"
     CDS             7587..7994
                     /locus_tag="VG272_00825"
                     /inference="COORDINATES: protein motif:HMM:NF042755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HWF12252.1"
                     /translation="MNETVKSETENGSQVMGIAAAIAGWVVPGLGHLILRRWTKAAVY
                     FVCVGALACAGLAMRGGVFGSGAEDLFDRLGFFADLGSGIFYFLAHQFQPAGPDVAHA
                     TGDYGTRLFAAAGMLNLLTVLEAYDIGHGGESK"
     gene            7991..8182
                     /locus_tag="VG272_00830"
     CDS             7991..8182
                     /locus_tag="VG272_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12253.1"
                     /translation="MTGLFGNLTHFDALIFFAVVVSITFAFLSKHANIERLKYALFAF
                     LAFMLVAIGIGWLMLPFSQ"
     gene            8413..8874
                     /gene="nuoI"
                     /locus_tag="VG272_00835"
     CDS             8413..8874
                     /gene="nuoI"
                     /locus_tag="VG272_00835"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HWF12254.1"
                     /translation="MSSIIQEAGETFLTLVKGFSVTFRNMLRKTVTENYPEEPVHFQP
                     RYRGIHVLHRDESGLEKCVGCFLCAAACPARCIYIEAAENTEAQRISAGERYASVYNI
                     DYSRCIFCGYCVEACPTDAITHGHGFELATSNINTMIYRKEKLLEKKPTTA"
     gene            complement(8910..9128)
                     /locus_tag="VG272_00840"
                     /pseudo
     CDS             complement(8910..9128)
                     /locus_tag="VG272_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            9470..9931
                     /locus_tag="VG272_00845"
     CDS             9470..9931
                     /locus_tag="VG272_00845"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWF12255.1"
                     /translation="MPDTVHPEIIELARQIATEHKLEPALVCAVIEQESAWNPWAVRY
                     EPGFLSRYVAPLYTAGKLSATETYMRSMSWGLMQVMGQVAREFGFKETSLAELCEPAA
                     GVEFGCRVLAARLAKAKDDVPAALQAWNGGANFNYAAEVLARKRNYSSRAV"
     gene            10240..10836
                     /locus_tag="VG272_00850"
     CDS             10240..10836
                     /locus_tag="VG272_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12256.1"
                     /translation="MKLSLHITALIAVAIALALLTDAWYSARHDSQQLAATLAAQNAA
                     IQQTTDREKQRDSQLSAALAAIQAQKQDVRTPAQASKQLAAVLPPLPLPISIHDPDLS
                     APLTPGEIPSTTISVPQPDLVPLYDELQDCRENKVQTDALQKDLSDEKSRSAALLHER
                     DAAISTAHGGAFLTRLKRSAKWLAIGIAIGAAAAASHR"
BASE COUNT         2714 a         3057 c         2925 g         2649 t
ORIGIN      
        1 cgagctggct cgagcccatg tactcaacgc cattcagcag aaacaaattt tcactcgtgc
       61 gccgtcccgc aacagaaaat gtatttccgt tgctggtgct ggtattggga cttttcagcg
      121 cgctgtaatt gatcgtgccg ggattgaggg cgatcaaatc gtcaaagctg cgcccgttca
      181 gcggtaattc tttaatttgc cgttcgtcga ccacccctga aatcgaagcc gtcgtaacat
      241 ttacgacggg agcctcttcg gaaacggaca tctgctgcac aagttcgccg acttccaggc
      301 gaaaattcac cacggcttcc tggccgactt cgagactcac gccggtccgc acgacggact
      361 tgaatccctg cttttccgct tttaattcct gggggccaag gggcaaggaa agaacgcggt
      421 agctgcctga atcgtcggta acggcagttc gagtcgctcc ggattcctga ctcttcacag
      481 tgatttttgc atctcgaagc acgccgccgg aggaatcctg gacatttcca gaaatcgaac
      541 cgcttacctg agcgtgcaca gccgagggaa atacagcaag cgcaatcagc cctaagctga
      601 aaagaacagc acgaaaatcc ccgcggacac caaacgctct gcaccccata aacgcaatcc
      661 ccccgaaaag agcgaattcc ctggttctga ccccacctat gcatgcgttg cggtcacggc
      721 ttcgatgacc tgccgatagt aagattcata taacggaatc acatcattgg cacaatactt
      781 tttcttggca ttcacgcggg caatttttcc catcgcccgg ccacgatctt cctgcgacaa
      841 aatttccagg gcgtagcgcg ctccgacttc tacttcgcgc ggcgtgaaga ggtagccatc
      901 gacgccgtgc tccaccactt ccggtaggcc gcccacattg ctcgccacaa cgggcacttc
      961 gcaggccatg gcttcgagcg cggcgaggcc gaacgattcc atatcggagg gcagcagaaa
     1021 taaatcagcc tggccgagga actgataaac gtggtcttgt ttgccaagga aatgaacgtc
     1081 gcgcgtaagt ttctttttgc gaatcagatg ttcggcggcg ccgcggtcag ggccgtcgcc
     1141 aatcatcacc aacttggcag gcattttttg gcgcacgata gcgaacattt cgatgacatc
     1201 cgtaatgcgc ttgaccggac gaaagttgga caagtgcatc agaattggtt cgccgtccgg
     1261 cgcccattct gagcgcaaag cctgattggg cttgcggcaa tacaaatcgc aattcacaaa
     1321 gttgggaatc acttcaatcg gacgcttgat atcaaattcc tcgatggtgc gttgctttaa
     1381 atattgagaa atggcggtta cgccgtcgga ttgctcgatt gaaaatttgg tgatcggcaa
     1441 aaagctgcga tcgttgccaa cgagcgtaat atcggtgcca tgcagtgtgg tgatgaacgg
     1501 caatcgtttg ggcgcggcca tcatgcgtgc gagcagggcg cttacggaat ggggaatcgc
     1561 atagtgaacg tgcagcaaat cgaggctttc ggcctcggcc acctgcatca tttttacggc
     1621 tagagagagc gcgtaagggg cgtggtcaaa caaggggtag ctggaaattt ccacttcgtg
     1681 aaactgaatc cggtcgttcg tgtccgttag gcgaatggga ggcgcataac tgatgaagtg
     1741 gatttcgtgg ccgcgagcgg cgagttccat gcccaattcc gtcgcgacga cgcccgatcc
     1801 gccgtatgtt gggtagcacg tgattccgat cttcatttaa ttcgggcgaa gggcccgctt
     1861 gctcctgagt ttgataactg cgtcattgcg cgagctgaga gcattttgga atctgtggag
     1921 caatttcatc taaatccccg cctctgatcc ctcgttttta ctcgggacaa gcgaggcggg
     1981 ctgcagcagc cgcgtggacc acatcagctg gcaaggccgg gtattgcaac gcctcaccca
     2041 attttgttca gagcaattcc tatttccagc ggcagtttag caaactcatc tgtaaaaaca
     2101 cgggacaatt tgtttgcgcc tgagttggca caatgttaga ttcccgaacc ttgaacgaaa
     2161 tgagcacacc atccatcact actcctgcta ttggacgcgc tggaaggctc caaggtgaca
     2221 ggattttatg tttcatgatc caggaaataa agctggcgga aataaatgcg accgaagggt
     2281 gcgaaatatc atgaagccgc gaggaaaact atccaaggct gacctggcga gagcggacga
     2341 gcgcttgcgt cacagcgccc cgatcaatca cttcggcttc caattattca aggcggagcg
     2401 aggtcgtgcg gtattcaaga tgcccgtgct cgatctgcac aagcagattc accgcgttgt
     2461 tcacggagga gtgctggcca tgctggcgga cacagccgga ggttttgccg cgtttctcgc
     2521 gtccccgcca ggctcaagag tggttacgat tgaaatgaaa attaattttc ttgaggctgt
     2581 tgaacatggt gagatcaaag ccgacgcccg cgtcctgcgg cagggaagaa ccactagcgt
     2641 ggtcgattgc gatgtaaccg accacgacgg gcgtctcgta agtaaggccc tgatgacatt
     2701 ttctgtatcg atgcagaaaa tgtaagagac tcaagcgggc atgtctccgt cttggctcct
     2761 gaaggcaaat ccatccgaaa caattggccc agtgcaacta tgtcctgaaa agtgacggga
     2821 ggccatcctg ggacagcgca agggccgcct tacgtgaata atggattatc cgttcgtgcc
     2881 gccgagggct gcctccgcac catccttgta aatatacttt atgaattgct tataaatcag
     2941 cagattcggg cttcctgtcc gagtaagctt aatgcaatct ttgtcgtacc actccacaat
     3001 accttgcaga ttctcaccat ccgtcatgac gatcaccacg ggcgtgcgcg cctgcatctg
     3061 cttgatccaa tagaagttct cggcgtgagt ttgctcgggg ggagcaggct tcttatgcgg
     3121 ttgaggccca tttgtagatg aatgagtgcg ggtggaactg ggatgaggtt ctttcatttc
     3181 cgtgagagta ggacgaaata gcttgcgatt cacgataaca actccttgaa ttatataatt
     3241 ccaccaacaa gatgaggctt ctcacctgct taagatgcaa accgaacgga tcagggtaca
     3301 cttccgttac cgagaaattg atttttttat gcccggcgag gggcgggtca gagatatggc
     3361 tagcgcatcc aatcccaatg accggtttac attccttcgt gttcgggcgt gaagtacatc
     3421 aaatccttcg actgctttat taatccacgc cacgtcaatc ttctggctga gcgtttcgag
     3481 ttcccggcgt aacgctggat ttcgtaacac cttcatcgtg gaagagctgg acaggtctag
     3541 caagtccgtg agcagactat agaacacttc caagacattt tcaaacgcga tctcctgact
     3601 ttttgccaac gctgaagtgc ttgcaaataa atcggaaacc ccgcgcaatt ctatagagcg
     3661 ttcgagaatt gttaacgcat cgcgatgcag tttggcgacg gcatccagat ccatgccgat
     3721 ggcagttccc gggcaacctt cggcatattg cgcggcaatt ttacgctgct ccggggtcat
     3781 tttcgtatga gtcgacagaa ttttttccac ttccgctgtg ccgagcggag agaatgaaaa
     3841 ttgcaggcag cgcgaccgta tcgtgggcaa tagttgatag gggctgggag cgagtaaaat
     3901 tagtgtcgat gactccggag gctcttccag gatttttaga aaaatattgg agaggtccca
     3961 gcgcatggtt tccgcgccgt cgataatgaa tactcgacgc cgcccacgtg gcttgaagta
     4021 ggctgcgcgc tgaaccgcgc gcagctgccc aacatggagc atcggtcgag ccactggatc
     4081 ttttgagcgg atcgggtctg gcacaatcgc ccagacatcg ggatgagtct ccagcaaaag
     4141 cggcacgcgc tctaccgtcg cggagtccgg gcgctcaccg cgctgcgcga ggccctggct
     4201 gatcaagctg gacgtatccg cgagcagcgc aattttcctg caatgttcac attcgccgca
     4261 aaaatcatcg cgcagccgtt cacagttggc tgcttgcgcg aacatccgtg cgagcgtaaa
     4321 tttccccacg cctctcggcc cggaaaaaag catcgcatgc gcaacgcgat ctgttgccag
     4381 catgccgcgc agcgcggtga ccacgcggga attgccgatg aattccgaaa agcccactaa
     4441 gtcatccacc tttccaagcc catactattt gtgtgacaaa attcgcgctt cgacacatcg
     4501 aagcacggat tgatgaatct cttccggcgc gcgaagtgat ctcgaactcg catccataca
     4561 gtcgatgcgc gcccaattgg cttcggttgc cagccgatcg taaattatcc cagtttgttc
     4621 cagatgcttg atgtcggctt cttgaatatc tcgtttcagc ctggtgtaat ccctcgccgc
     4681 tttcagtccc acgaggcgat gcgcctcttc aaatggcacg cgcaaataaa tcaccaggtc
     4741 ttccgccggc aaaccataaa gtgtgtattc aagatgtttc aaccaggcaa aaaattcctc
     4801 gcgcttttcg ggcgcaaccc gcgcactttg gtgcgccatg ttggaaccga tgtagcggtc
     4861 cgcaagaaca atcttgcccg ctactaaagc agcttcaatc tcgggcctcg cttccaggcg
     4921 atccccggca tagagcagcg cggacaaatg tgggtctacg gcgtcgagcg tgccaaattc
     4981 tccattcaag taaagcccca caagctttcc atagaacgat tcgtaccgcg gaaagctgat
     5041 gcgatgagta gggaattttc gctcttcgag cgcggttgcc agcagttcaa gttgcgtgcg
     5101 cttgcccgat ccgtcgattc cctcgatcgc aataagtttt ccgcgggtgt ccatattgga
     5161 tgtgccagtc tagcgtgagc gcacaatcct cgcaaggaat gcgcgccagt tggagatttt
     5221 ttataaattt gcaaattcct ctgccacaag cgcgccgagg cacattggac tcagcatggt
     5281 caaagacgag cgtgctagaa ttcgccgtat gggtttcgca tcagtggaaa ttgagaaaaa
     5341 ttttgccgac gtactggaac gcattgaccg ggctgcttct cgcgccgggc gtcgagcgga
     5401 ggaaattaca ttagtcgccg tcaccaaaac tcatcccgca tctaaaattc taagtctgta
     5461 cgaagccggg gcgcgccact tcggcgaaaa tcgcgtccag gaacgtgaat cgaagctcgc
     5521 cgaaaccgtg aaagttgacg ccacatggca catgataggc cacctgcaaa aaaataaagt
     5581 tgcgcgcgtg ccgcgcatgt ttgacagcat cgacaccatc gacagcatca cgctagccga
     5641 aaaactcgac cgcgccgcgg gtgagttttt gagaagtaaa aatgaaaatg gcgatttgct
     5701 tccgcacccg tacgatcatc gcctccgagt attgatcgaa gtgaagttgg atccggagct
     5761 agcgaaatcc ggcgcttcga acgaagagtt gccccgcctt gttgaaggcg ttttgcggat
     5821 gccacattta catttgcagg gccttttcgg ggtgccgcca tatttcgatg atcctgaaga
     5881 cgcgcgccca tatttccgtc atctccgcga attgcgcgat aaaattcgcg aaggaatggg
     5941 cgcgcaatta ctgcctgacc tttctatggg catgtctcat gatttcgaaa ttgcaatcga
     6001 agagggcgca acagaagtcc gcgttgggac atcacttttc gggcaaaggg aatacgcgga
     6061 atgagcctgg agattgccga gaaaaatggt tcggttagtt ttcacgttcg cgtgcaaccg
     6121 cgagcaagct gcgatgagtt tgccggcgaa tatcaaaacg gtctgaaaat tcgccttacc
     6181 gcgcctccca ttgacaatcg tgctaatgaa gcgttgcgaa aattcctggc attgcgtttg
     6241 aatgtgccta tgacggctgt tagaattgcg gcaggtgagc acagtcgcac caagcgtgtc
     6301 gaaattagcg gcgtttctat ggcaacgatt cgcgccctcg tggattaaaa cagagtcgtt
     6361 tccctcgaca acgggtcctt cagcgtaaat ttcctatgcc aaacatcgaa gctatccagg
     6421 ccgcgctccg ggagcaggga atagacggat ggcttttcta cgatattctt catcgcgacc
     6481 ccattgctta tcgagttctg ggccttgacc atgcgttagc caagcgccgc tggttctaca
     6541 tgattcccgc aaaaggcaat ccgcgtaaac ttgtgcaccg catcgaagcc gcaactctcg
     6601 attcgctgcc cggcgaaaaa ttactttatg ctgcggccgg cgaattggaa aaaaatctac
     6661 cgaaactcat tggtcgcgcg aaaaaagtgg ccatgcaata ctcgccgaaa aattcaattc
     6721 catacgtttc gcttgtcgat gccggaaccg tggagttgat tcgcggtcag ggttgcaagg
     6781 tggttagttc ggcggaactc gttcaacaat ttgaagctgc gtggactccc gaacaacttg
     6841 aaacgcatcg ggccgcaggc cgctcgattg actccatcac gcaagccgca tttgctgagg
     6901 cagcgcgaag ggtttccgcc ggcgaaaatt taactgaata cgatttgcag cagtggattg
     6961 tcgcgcagtt tgacgcacac ggagtggttt ccgactctcc cccgattgtt gcggtgggcg
     7021 cgcattccgg taacccacat tacgagccaa tggcgcaggg ttcggccatc atccgtcgtg
     7081 gtgatttcct cctcctggat atttgggcga aaacgcaaac tccgaacagc gtctactacg
     7141 acattacgtg ggtcggatat cttggctcaa aaatccccgg caaatacgcc aaaattttct
     7201 cgattgtccg cgaagcccgc gactcggccg tgcactttgt ccagaagaat attgctgctg
     7261 gccgcaatat cgcaggctgg caagtggacg gcgttgcgcg tgaggttatt cgcaaggcgg
     7321 gatacgcaaa atattttgtc catcgcacag gccacaatat cgggcaggag gttcacggcg
     7381 caggagcaaa tatggatggc ttggaaacgc gagacattcg ccgcataatc cctcatactt
     7441 gcttttctgt cgagccgggg atttatctgt cggaatttgg cgtgcgcagt gaagtggatg
     7501 tctacgtcgg gcaaaatacc gcggaagtta ctggacctgc gcagatggag atcgtaaaac
     7561 tattctcgta aatgtgaggt accagagtga atgaaactgt aaaatctgaa accgaaaatg
     7621 gcagtcaggt catgggaatt gccgcggcta tcgctggttg ggtcgttccc gggttaggtc
     7681 acctgatact tcgccgttgg accaaggccg ctgtttattt tgtatgtgtg ggcgcgttgg
     7741 cttgcgcagg ccttgccatg cgaggcggag tctttggctc tggtgccgaa gatttattcg
     7801 atcgcttggg attttttgca gacctcggat ctggaatttt ctattttctc gcacatcaat
     7861 ttcagcccgc cggtcctgat gttgctcacg caacagggga ttatggcacg cgcctgttcg
     7921 ccgccgctgg catgttgaac ttgcttactg tcttggaagc ctacgatatc ggccacggcg
     7981 gagaatcgaa atgaccggtc tgtttggaaa tcttactcat ttcgacgccc tcatattttt
     8041 tgcagtggtc gtttcgatca cttttgcatt tctttctaaa cacgcaaata tagagcgact
     8101 taagtatgca ttgtttgcgt ttcttgcgtt catgcttgtg gcgatcggaa tcggctggct
     8161 gatgcttcct ttctcgcagt agattcggct ttagccatac tagggaaaag tatgaaggaa
     8221 attttccggc tttcggatct gcgatgatcg gtttattatt ctgtcaatgc gagcgaagca
     8281 atgattctcg ccttattttt cctcaacccc tttaacaacc ctcggaatcc aatatgtctc
     8341 ttgatcaact gagtatcagg aacttccgcg tcgatttgcc ctgtggcatc gactttgcta
     8401 aactcatggc cgatgtcatc catcattcaa gaggctggcg aaacatttct tactctggtg
     8461 aagggtttca gcgtgacatt ccgcaatatg ctgcggaaaa cggtcactga aaattatcct
     8521 gaagagccgg tccatttcca accccgttac cgcggcatcc acgtcctcca ccgcgacgaa
     8581 agcggtctgg aaaagtgcgt cggctgcttc ctgtgtgcag ctgcctgccc agcaagatgc
     8641 atttacatcg aagctgctga gaatacggaa gcgcaaagaa tttcggcggg agaacggtat
     8701 gcgagcgttt acaatatcga ttactctcgc tgcatttttt gcggctactg cgtagaagcg
     8761 tgtccaactg atgcgattac acatggccac ggttttgaac ttgccacatc caacatcaac
     8821 actatgatct accgcaaaga aaaactgctt gagaaaaagc ccacgactgc ctaagtgttt
     8881 tacgtcacaa ccagaatcta gaagccatct cataaatgcc tcagcgagat aatgaggcag
     8941 ccgagttgga gcatgccgag ggaattgggt tcatgatatt cccagcggct gaccagtcgg
     9001 cggaaattgt ggagccaggc aaagtggcgc tccattttcc agcgccgaca gtagcgacga
     9061 agttcgcggc catcctgggt gttcttgcgg cggcggttgt acttgtgagg cgcgatgagc
     9121 cggatgccgt atttttgcca attatttttc tttattcgct ggctgcacag gcgaacgcgc
     9181 gatgacgaaa tccgccgcgc cttcatccgc gacatggcgc tgaatcatct cccgcatctc
     9241 tatcacggct tgcgccaaat cggcgaccac ctcggcatca aactcaaaga gccgccccga
     9301 gttcaattcc tggaactaaa cggcagcgaa agaaaatggt gattgagtgg cgccagcttc
     9361 cgtctgcggg gtttagtgca agcgcaatcg agagtatgag taataaaatt ccacggccta
     9421 aatcccgcat cccagaacta cgaatcacaa ttcacaaatc actcccaaca tgcctgacac
     9481 cgtccatccc gaaatcatcg aactcgcgcg ccaaatcgcc accgagcaca agctcgagcc
     9541 tgcgctcgtc tgcgccgtca tcgagcagga gtccgcgtgg aacccgtggg ccgtgcgcta
     9601 cgagcctgga tttttgtcgc gttacgtcgc gcccctctac acagctggaa aattaagcgc
     9661 cacggaaacc tacatgcgct cgatgtcgtg gggactcatg caggtgatgg gacaagtagc
     9721 aagggaattc ggcttcaagg aaacttcgct ggcggaattg tgcgagcccg ccgccggcgt
     9781 ggaattcgga tgccgcgtcc tagccgcgag gctggcaaaa gcaaaagacg atgtccccgc
     9841 agccttgcaa gcttggaatg gcggagcaaa ttttaattac gcggccgaag tgttagcgcg
     9901 aaaacgcaat tactcatcgc gcgccgttta gggaagcctt gcatggcgcg ggcttcagag
     9961 tttgggtgcg aactgcggaa tcgttgtggg tcgtggcttt agacacgaca tgagccctgc
    10021 aaaatgagtc cggctttggc tgctgaagtt cctatgttgc agcatccaca aagactcttc
    10081 agcctgatcc cggcgctaga agaaattaat atgtaagcaa taaatccaag agggaagtgc
    10141 gaaacaggaa ttccccgcca acgtttctca ggtgagacgc gattcacgaa ctgtgaatca
    10201 cgattcacga aacggaaatc acgattcacg gttcacatta tgaaactatc ccttcacatc
    10261 accgccctga tcgcggtcgc tatcgcccta gctcttctaa ccgacgcatg gtattccgcg
    10321 cgtcacgatt cgcagcagct cgccgccacg ctggcagccc agaacgccgc aatccagcaa
    10381 accaccgacc gcgaaaaaca gcgcgattcg caactctcag cagccttggc cgcaatccaa
    10441 gcccaaaaac aagacgtgcg aaccccggcg caagcctcca aacaattagc cgccgttctc
    10501 ccgcctctcc cgctgcccat atcaattcat gacccagatt tatccgcgcc gctaacgccc
    10561 ggtgagatcc cctcgacaac tatttcggtc ccgcaacccg atctcgtacc tctctacgat
    10621 gaacttcagg attgcaggga aaataaagtc cagacggacg cgctccaaaa agatctgtca
    10681 gacgaaaaat cgcgctctgc cgcattgctc cacgaacgtg acgccgccat ctccacagcc
    10741 cacggcggcg cattccttac gcgcctgaaa cggtccgcaa aatggttagc catcggtata
    10801 gccatcggag ccgccgccgc agcatcccat cgctaaccgt ttcctcacaa aaaatttgcg
    10861 aaatggaacc ccaaaagccg taagcctcca gctcccacag agcaagaggt cagcagaaag
    10921 accttcacca attctggttt ttcaattttc tccgtaccta aataagaaat atagaacgcg
    10981 cgatgtattc acaattcttt cttcacatct tctcgcaatc aaaatcatcc agcaaaccaa
    11041 tccaaataat ttcagcgact tcccagcccc gtcctcacct aatccaacta aaaaacccta
    11101 tgaaaccccg gccatctctc cacagactgc tcccaaaatt acagccctaa aagtagcaaa
    11161 taaaagcttg acatcaagca tccgataagc tatattatgt ttatcattgg ttggtttcgt
    11221 atgtggaggc ttgctctagg gtggcttggt cgggacctac tactcgacgg gcgttcacga
    11281 atgttgtggg tgtggctgtt atggttaggg cgtgaccttc ctccatcgtt tcttttactt
    11341 cattt
//
LOCUS       DASZMB010000022        11558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_565425, whole genome shotgun sequence.
ACCESSION   DASZMB010000022 DASZMB010000000
VERSION     DASZMB010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 11558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11558
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565425"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..73)
                     /locus_tag="VG272_00855"
     tRNA            complement(2..73)
                     /locus_tag="VG272_00855"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38..40),aa:Gln,seq:ttg)
     gene            complement(331..1338)
                     /locus_tag="VG272_00860"
     CDS             complement(331..1338)
                     /locus_tag="VG272_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HWF12257.1"
                     /translation="MAVFLVKTISGLVAILLLAILGLNLYVASRPAGKTMRAAGVISI
                     PVPFHLGRPFIDYMTIHGSRLYAGYASEGLVGVVDTKTNRVISTINGLTRVHGIAIVP
                     DRNLGFASSSGDNVVGVFDLNSDQLLQKIPAGDGADAIIYDAQARMVYVANHTGKTGM
                     LIDPGALKVITTIPLGGEPEYPQADPETGLIYQNLEDTSELVVIDPQKQAVIKRYKLD
                     PGEGPTGLALDAVHHRLFSATGNKKLIVLNAETGAIVSTLPIEAGCDGAGYDPVLNRV
                     YTANSIGTMTIIEQDSPDRYRVLENARTHLVGHSLVVDPVTHRIYVAYFGSIAAYDPV
                     K"
     gene            complement(1615..1950)
                     /locus_tag="VG272_00865"
     CDS             complement(1615..1950)
                     /locus_tag="VG272_00865"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWF12258.1"
                     /translation="MQLSMEELEGEITKVILDGRMDIAGAADVDLKMSVIAGSKKFVV
                     IDAQKIAFLGSMGLRTLVNTMRAIKSRGGKIVLYGANQEVEKVLTVSGVSTLLPVVQD
                     FQSAIALMQ"
     gene            complement(2164..2253)
                     /locus_tag="VG272_00870"
     tRNA            complement(2164..2253)
                     /locus_tag="VG272_00870"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2217..2219),aa:Ser,seq:cga)
     gene            complement(2343..2924)
                     /locus_tag="VG272_00875"
     CDS             complement(2343..2924)
                     /locus_tag="VG272_00875"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HWF12259.1"
                     /translation="MRFLHAVSGFFLFFELPIPIYWVILHPFNSFWRTRVRAAFWFAG
                     LFAWISGGALLWLFRNRLFANVHPSWFEIIAGFLLIFVEFYLLARVERELGSRRLVGH
                     SELTGTGEFFAGGLYAYVRHPRYAGMFSAVIGSALLCGTPLLWMVIVLWFPFALIVIG
                     LEEHELMARFGPSYEAYRKRVPAFFPFGKNAKK"
     gene            complement(2925..3134)
                     /locus_tag="VG272_00880"
     CDS             complement(2925..3134)
                     /locus_tag="VG272_00880"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaP-like transmembrane domain"
                     /protein_id="HWF12260.1"
                     /translation="MLAETNVSGTARALYLVIGAAFAIWGLWGADQGWTQWIWLTLGG
                     LLLVLGIIGYSPVHSILAKNHQKAN"
     gene            3344..3700
                     /locus_tag="VG272_00885"
     CDS             3344..3700
                     /locus_tag="VG272_00885"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWF12261.1"
                     /translation="MSTLEKTSWNSIEKENLTDRISRQMISGESATVSQLFLKKGATV
                     PRHSHVNEQYSWIMSGSLNFIFDDREVFVGAGEILIIPANVPHGAVALEDTLDVDIFT
                     PRREDWIRKDDAYLRK"
     gene            3874..6465
                     /locus_tag="VG272_00890"
     CDS             3874..6465
                     /locus_tag="VG272_00890"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HWF12262.1"
                     /translation="MSRTFLSAAILGLAILPANVARADRPYAPSREYDLQNVRVSLHF
                     DIDQRKVIGDVTHTLATLRDGETHLDFDCAELSVSSVRVNGKDATFELRDNNLRVQLA
                     TAAKAGEKFEVEIKYEGRPTSGLYFILPDKGDPNRAKEIWTQGEAEDTHHYIPIYDYP
                     NDRTTFEMILTVPADWLTVSNGKLLSVQDAPNGQKTWSWRQSQPVSTYLISFVAGEYQ
                     SKKDNWRNIPVSYNVPRGMGDTIDSTFSHTKQMLDFFSERFGVIYPWEQYAQTAVHDF
                     VASGMENVSATTLAARDMLPADLAAERPEAADNLVSHELTHQWFGDLVTCKDWTNTWL
                     NEGFATFGAELWEEHFYGADASGYRYWREQNNWMQSRELYPIPIVTRNIDDSVEYEGN
                     VYDKAGWVIHMLREKMGDDAFFRALKHYLEANRLQNVVTADLVKDIEESSGTNVDQFF
                     DQWIYGAGAPRFTVRYTYDDAEKKVILSVKQTQKVEGYVGLFRVPVDVSITTSSGEKL
                     FPIEVSKADESFSFPVDGAPQIVLFDKGDRILKSVDFQKTPDEWMRQLRTASSVPDRA
                     DAAVALGNYHDNDAAVNALGEVALHDKFWGVREEALRSLGRINSPAARKQVLADLANE
                     QPWVRVVAVEQLGKDRGDEDLLKRLQNIYKDDKAYTVRAAALQSLALDKAPNLEATLE
                     KTLTISSPDDVLRSAALRAMGSLGDDSAVPTLLDWSSPGKSAQLRGVAIGALGRVDLK
                     NHDITARLISYLNESSFDIRFATIFALGRRGDPTAIEPLEALLKTGQLSIGVPHAVEN
                     LIEELKTKSAPQQGPPANGQKGGDSAASNSNQALLDRLDRLEHQLTDMNEKLQRIESS
                     LPGSKTD"
     gene            complement(6586..7905)
                     /locus_tag="VG272_00895"
     CDS             complement(6586..7905)
                     /locus_tag="VG272_00895"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HWF12263.1"
                     /translation="MADLKQCALQIFRETLAGIDIPASMQRQLCRAGSQIFVNGAPYD
                     LAAYEQILAIAIGKASLAMAHGLSETLSPNFRAEGIVVGPTAPSNLPDGFRSIVAGHP
                     IPNIGSFSAGRAILDLLARATERTLVVFLLSGGGSALVELPLDPAVTLEDFRELNRTL
                     VTCGASIDEINAVRKHLSAVKGGRLAVAAGDATKITLGVTDVPEGQESALASGPTLPD
                     PTTVADACSVVQRYSLFSKLPLSIRTRFEHPEFILETPKPDSVAFDPRRSTFQILLGR
                     HALFHTAHHASESLEFVTICDNTTDNWPVEKSADFLLEQLVALKESNPHKSVAVIADG
                     EVSSPVTGDGVGGRNLAFVLDCVKKIAGRKIAVLSVGTDGIDGSSPAAGGVADGESLS
                     RAQALGLDPADYFSRSDSYTFFKKLGDAIETGPTGNNLRDLRIFLAE"
     gene            complement(8029..8463)
                     /locus_tag="VG272_00900"
     CDS             complement(8029..8463)
                     /locus_tag="VG272_00900"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HWF12264.1"
                     /translation="MAHVSLNHPKLTLEGAEAILDAAKAQAARIGVPMNIAVVDDGGF
                     LMAFARMDGAKPASIDIAFNKAHAAAIRRQNTGPARSGDEVSVLLSLGLAIGSRAHQT
                     PIRGGLILEVSGQVVGAIGVSAGSEDEDTEVARAGVAAFVKG"
     gene            complement(8527..9519)
                     /locus_tag="VG272_00905"
     CDS             complement(8527..9519)
                     /locus_tag="VG272_00905"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HWF12265.1"
                     /translation="MSKPKLLSTHLLFEGPRKKLEEYFEVEYWKGDERPSREEFLKRV
                     AGKDALICLLTEKIDDEMLEIAGPNLRIVANVAVGYDNIDVLACTKRNVAVTNTPGVL
                     DETTADFTWTLLMAVARRLIEADHMARSGAWKKWNLDQLCGTDIWGKTLGIIGFGRIG
                     RAVTRRALGFRMRVIYSSTSRAPLEIEKELNAEYVSRDAVFEQADFVSLHVPLNPATR
                     GLIGPQELAKMKATAFLINTTRGPVVQEAALIEALERGLIAGAALDVFEREPIIPDGL
                     RRDNVVLAPHLGSASIETRTKMAMIAVENAIALFAGQRPPTILNPEALLKTSNH"
     gene            complement(9664..10023)
                     /locus_tag="VG272_00910"
     CDS             complement(9664..10023)
                     /locus_tag="VG272_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12266.1"
                     /translation="MGLFSKLFGSKNSENDGPPVQEVEIHFAYGSTNFQHVYSFGDVL
                     QQAIAEAKVGEYDGHALPADGSEGRYFVYGPDAEAIYKAIAPVLQASPLMRGATVTLI
                     YGPRRRGTPKRVIELPG"
     gene            10308..>11558
                     /locus_tag="VG272_00915"
     CDS             10308..>11558
                     /locus_tag="VG272_00915"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWF12267.1"
                     /translation="MQQQLIRELEALLGPKAVLSSAEDLMLYEYDGSVELGSPRCIVF
                     PSSARDVSGIVKLANRYETPIVGRGAGTGLSGGAIARSGGIVIAFSRMNRILEIDGAN
                     LRAVVQPGVVNLDLSRAVEHLGLFFAPDPSSQKACTIGGNVSENSGGPHTLAYGVTTN
                     HVTGIELVLTDGEIVRIGGKNIESAGYDLGGLLVGSEGTLALVTEITVRLMRKPEAVK
                     TLLSVYDTLDDTTESVVDITVRAITPAACEMLDGFTLRAVEDYVHAGFPKDCAAVLLI
                     EVEGLKEAVEAQAAEITEVCREHRAREVRAARTDQERNLLWRGRKEAFGAMGRISPMY
                     YVQDGVIPRTRLPETLRRIEEISKKYDLQIGNIFHAGDGNLHPLILFDERNAAQFARV
                     VAAGGEIMRHCVEVGGALTGEHGIG"
BASE COUNT         2763 a         3096 c         3061 g         2638 t
ORIGIN      
        1 gcttggggac ttggactcga accaagatta acggcttcaa aggccgctgt gctaccgatt
       61 gcaccatccc caacccgctc aactggaccc tgacgctttc ttgtggccca ttctcttttg
      121 catcgcttca agctgacata cttttgaggc gatattgtaa cgtatgaata tgcgaattgt
      181 cttgtccgtg aaattttgat tgcggttggc atggcccctc gtccgtcaat ttcgaattgc
      241 ccgcgcaaaa attgaatcat aggagccgaa aagaggcctc tccgtcccta gctcttcagg
      301 acggagagcg cgctgggcca ttccaaatat tcactttacg ggatcgtagg cggcgatcga
      361 tccgaagtag gccacgtaaa tgcggtgcgt gacagggtcc accacaagcg aatgaccaac
      421 cagatgcgtg cgtgcatttt ccagcacacg gtatcgatcg ggagaatcct gctcaataat
      481 agtcatggtg ccaatgctat tcgcagtata aacccggttc agaacgggat cgtagcccgc
      541 gccatcgcag cccgcttcaa tcggaagcgt agaaacaatc gcaccggttt cagcattaag
      601 gacgatcagc tttttgtttc ctgtggcgct gaataggcga tggtgaacag catccagcgc
      661 caagcccgtg ggaccttcgc cgggatccag tttgtagcgc ttaatcacag cctgcttctg
      721 tggatcaatt accactaact cgctcgtatc ttcgaggttt tgatagatca ggccggtttc
      781 aggatccgcc tgggggtatt cgggctctcc gcctagcggg atcgtggtga tgactttcaa
      841 cgctccggga tcgatcaaca tgccggtctt tccagtatgg ttagcgacgt aaaccatgcg
      901 agcttgtgca tcgtagatga tggcatctgc accgtccccg gccggaattt tctgcagcag
      961 ttgatcggag tttagatcga aaacgccaac tacgttatcg ccgctcgaag atgcaaagcc
     1021 gagatttcgg tcaggcacaa tggcaattcc gtgcacacgc gtaagcccgt tgatcgtgga
     1081 tataacccga tttgttttgg tatccaccac gccgacaagc ccctcgctgg cataacctgc
     1141 gtaaagccgc gagccgtgaa tggtcatgta atctatgaac ggtcgcccca gatgaaacgg
     1201 cacaggaatc gaaatgacgc cagccgccct catggttttg cccgccgggc gtgaggctac
     1261 gtaaagattc aggccgagta tcgccaaaag caggattgcg acaagtccag aaatggtctt
     1321 cacgagaaat acggccattt ttctcaattg ttcctccttg tcctttagtg aacccttggc
     1381 agttcagatg gcattttggg ccaccaatcg tggctttctt ttgcaagcgc tgcaatcgtt
     1441 tacgcaaggc ggtatcgatt tgtttcacca cgccattcct gaaagggagg aagcctggtc
     1501 aaatcacctc gttcactagg catagttcag attgtctttg cgagtttgta ttgcctgttc
     1561 gctgtggatg agtgattcaa aggcagcagt attgtaaccc gataacgtgt tacttcactg
     1621 cattaatgcg atggcactct gaaaatcttg aacaactggg aggagcgtgc tgacaccgct
     1681 taccgtcagg actttttcga cttcctgatt cgcgccgtaa aggacaattt tgcccccgcg
     1741 actctttatc gcgcgcatcg tgttcacaag tgttcgaaga cccatcgatc cgagaaatgc
     1801 aattttctgt gcatcaatta ccacaaattt tttgctgccg gcaatcacgc tcatttttaa
     1861 atcaacgtcc gcagcgccgg caatatccat ccggccgtcc aagataactt tagtaatctc
     1921 gccttcgagc tcttccatag aaagttgcac gctgagttgc ctcctgagtg atgaagcaca
     1981 attccgaaat tcaatttgcg cgcgatgatt cggctgatgt ttgcgttccg gcgccaaatg
     2041 atcatagcgc cgcgtatgtt acagaattgt tacaagccaa tcctttgcca tcccgagagc
     2101 cgaatcaatt ctctggagta acaataattc atgattgcac atgagtggaa gaaacagacg
     2161 atatggcgga gagggtggga tttgaaccca cggtcgggtt tccccgacac tcgctttcga
     2221 ggcgagctcc ttcaaccgct cggacacctc tccgcagagg caaaccattc tatcggagct
     2281 tgaagaactg ggcaacgata gtgatgtggt agattcgggg attgtttcct tcggaatgat
     2341 ttttactttt ttgcattttt gccaaatggg aaaaaggcgg ggacgcgctt acgataggct
     2401 tcatagctag ggccgaagcg agccatcaat tcgtgctcct cgaggccgat gacaatcagg
     2461 gcgaacggga accagaggac tatgaccatc cagagcagcg gtgttccaca tagaagcgca
     2521 gatccaatga cggcactgaa cattcccgca taacgcgggt ggcgaacgta agcgtacaat
     2581 ccgccggcaa aaaactcgcc cgtgcccgtg agttcggagt ggcccaccag cctgcgactc
     2641 ccgagttcac gttccactcg cgcgagtaga tagaactcca cgaaaatcag cagaaaaccg
     2701 gcaatgattt caaaccagga tgggtgcaca ttcgcaaaca acctattccg aaatagccat
     2761 aacaacgcgc cgccgctgat ccacgcgaaa agtcccgcga accagaatgc agcgcggact
     2821 cgagttcgcc agaacgaatt gaagggatga aggatcaccc agtaaatggg aatgggaagc
     2881 tcaaaaaaaa gaaaaaatcc agagacggcg tgaaggaacc gcattcagtt tgctttctga
     2941 tgattcttcg ccaggatgga gtgcacagga gaatacccaa ttataccaag gactagcagg
     3001 aggcccccca aggtaagcca gatccattgc gtccagccct ggtcagcgcc ccacaaaccc
     3061 cagatggcga aggccgcacc gattacgaga taaagtgccc gcgcggttcc cgaaacattc
     3121 gtttctgcta gcatgccatg gcctcggctg ttggtatcat tcagcgcctc gcatgttacc
     3181 agatgtttcg catatcatta attttgccga atcatcccac gctaaaacgt ggcggttgcc
     3241 gcggactcca agttgcccag tacgatttac gcaccgggtt gacgccgcgt gcgtctccgg
     3301 tagaggatgc tgcacaagat aatttcaatt tgaggaggca tcaatgagca ctcttgaaaa
     3361 gacatcctgg aactcaatag aaaaggaaaa cctgacagac agaatttcgc gccaaatgat
     3421 ttccggtgaa agtgccactg tttcgcaact cttcctaaaa aagggcgcca ccgttccgcg
     3481 ccattcgcat gtaaatgagc aatattcatg gatcatgtcc gggtcgttga attttatatt
     3541 cgatgaccgg gaagtttttg tcggcgcggg tgaaatcctg attatcccgg cgaatgtgcc
     3601 ccatggagcg gtcgctttgg aagacaccct ggacgtcgat attttcacgc cacggcgcga
     3661 agactggatt cgtaaggatg acgcttactt gcgcaagtaa tcctggccgc gcgccacgac
     3721 ccaggtaacc atttaggcag attcgcgtct aatgacaatg ggaaaaaagc ttgcactcat
     3781 tccgcaaggg aagcacaggt ataatggttc gatgtttttg attgccgcaa attccctcgc
     3841 gaggcaggaa tgaattcgtc ctggataaag tttttgtccc gaacctttct atcggccgca
     3901 attcttggcc tcgccatcct tcctgcaaac gttgctcgcg cggaccgtcc gtatgcgcca
     3961 agccgcgagt atgacctgca gaatgttcgg gtctcgctcc attttgatat cgaccagcgc
     4021 aaggtaatcg gcgacgtgac ccatacgctg gctacgctac gcgacggaga gactcacttg
     4081 gatttcgatt gcgccgagct ttccgtttcc agcgttcgcg tcaatggcaa ggatgcaacc
     4141 tttgagcttc gcgataataa tttgcgggtt caactcgcaa ctgctgccaa ggcgggagaa
     4201 aaattcgaag tcgaaataaa atacgaagga aggccaacct ccggtctcta tttcattctt
     4261 cccgataaag gggaccccaa tcgagccaag gaaatttgga cgcaaggcga agcggaggac
     4321 acacaccact acattccaat ctacgattat ccgaacgatc gcacgacttt tgaaatgatt
     4381 ctgacggttc cggcggattg gctcacggta tccaatggaa aactgctgag cgtgcaggac
     4441 gcgcctaacg gtcagaaaac atggagctgg cggcaatccc aaccggtctc gacctacctg
     4501 atttcatttg tggcgggaga gtatcagtcc aagaaagata actggcggaa tattcccgtt
     4561 agctacaacg tcccgcgcgg gatgggcgat acgatcgatt ccaccttttc gcataccaag
     4621 caaatgctcg acttcttttc ggagcgattc ggcgtgatct atccgtggga gcagtatgcc
     4681 caaacagctg tgcacgattt cgttgcctcc ggcatggaga acgtaagtgc aacgactctc
     4741 gcggcgcgcg acatgttacc cgccgatctc gcggcggaaa gacccgaggc cgcggacaat
     4801 ctcgtttccc atgaattgac acatcaatgg tttggagatc ttgtcacgtg caaggattgg
     4861 acgaacacct ggctgaacga agggttcgcc acatttggcg ccgaattgtg ggaggagcat
     4921 ttttacggcg cggacgcgtc cggctaccgc tactggcgag aacaaaataa ttggatgcag
     4981 tcgagagagc tttatccaat tccaattgtg acgcgcaata tcgatgattc cgtggaatat
     5041 gaaggcaacg tttacgacaa agccggatgg gtaattcaca tgctgcggga gaagatggga
     5101 gacgatgcat tctttcgcgc gctcaaacat tatctcgaag cgaatcgcct gcagaatgtg
     5161 gtcaccgcgg atttagtcaa ggacatcgag gaatcatccg ggacgaatgt cgatcagttt
     5221 tttgaccaat ggatttacgg tgcgggcgcg ccgcgcttta ccgtgcgata tacgtatgat
     5281 gacgccgaaa aaaaagtaat actcagcgta aaacaaacgc aaaaagtgga agggtatgtc
     5341 gggctgtttc gcgtgccggt cgacgtttcg atcaccactt ccagcggcga gaaacttttc
     5401 cccatcgaag tctccaaagc cgatgaatca ttttctttcc ctgtcgatgg agcgccgcag
     5461 atcgtgcttt tcgataaagg cgatagaatt ctaaagtccg tcgatttcca aaagacccct
     5521 gatgaatgga tgaggcagtt gcgaacagca tcgagcgttc cggatcgcgc ggatgcggcg
     5581 gttgcattgg gcaattatca tgataacgat gcagcggtga acgcgctggg agaagtagcg
     5641 ctgcacgata agttttgggg agtacgcgaa gaagctcttc gctcgctggg gcgcatcaat
     5701 tcgcccgcgg cgcggaaaca agtgttggct gatcttgcca acgagcagcc gtgggtgcgt
     5761 gttgtagccg tcgagcagtt gggcaaagat cgtggcgacg aagatttgct caagcgcctg
     5821 caaaatattt acaaggacga caaggcttac accgtgcgcg cggccgcctt gcaatcgctc
     5881 gcgctggaca aagctcccaa cctggaagcg acgcttgaaa aaactctgac gatttcttct
     5941 cccgacgatg tgctgcgaag tgccgcgctt cgcgctatgg gatcgctggg tgacgattcc
     6001 gctgttccca cgctcctcga ttggtcttcg ccaggaaagt ccgctcagct ccgcggcgtc
     6061 gcgattggag cgctggggcg cgtggatctt aagaaccatg acatcactgc gaggctgatt
     6121 tcctatctga atgaatcttc gttcgatatt cgttttgcaa ctatttttgc gctggggcgg
     6181 cgcggggatc ccacggcaat tgaaccgctt gaggcgctgc tgaagacggg ccaactctcg
     6241 atcggagttc cgcacgctgt ggagaacttg atcgaagagc taaagaccaa gagcgcgcca
     6301 caacaaggac cgcctgcaaa cggccagaaa ggtggcgatt ccgccgcatc aaacagcaat
     6361 caagcactac tcgaccgtct cgatcgtctt gaacatcaac tgacagatat gaacgaaaaa
     6421 cttcagcgca ttgaatcttc gttacccggt agcaagaccg actagaaata atcaccaaga
     6481 ttatggatcg gttttggtgg cgcgggctgt agcctacagg cttggttctt gcaaggccaa
     6541 aattccgcag gccgaagccc gcgccacgta agcctcatca aacgattatt ctgcgagaaa
     6601 gattcgcagg tcgcgcagat tatttccggt tggacctgtt tcaatcgcat cgcccagttt
     6661 tttgaagaac gtgtaagaat cgctgcgcga aaaatagtcg gcgggatcga ggcctagagc
     6721 ctgcgcccgg gataaacttt cgccatccgc gacccctccc gccgcagggc tagaaccgtc
     6781 aattccatcc gtgcctacgc tcaaaaccgc aattttgcgg ccggcgattt tcttcacgca
     6841 atcaagcacg aaggccagat ttcttccccc aacgccatcc cctgtgacgg gcgaactaac
     6901 ttcgccatcc gcaatcaccg cgacagattt gtgcggattg gattccttca gcgccacgag
     6961 ttgttccaga agaaaatcgg cagatttttc cacgggccaa ttatcggttg tgttgtcgca
     7021 gatggtgacg aattccaagg attccgatgc gtgatgcgcg gtgtgaaata atgcgtggcg
     7081 accgagcaaa atttgaaacg tcgagcgacg cggatcgaat gcgacactat ccggctttgg
     7141 ggtttccagg atgaattcgg gatgctcgaa ccgcgttcga atgctgagcg gcaatttcga
     7201 aaacaagcta tagcgctgaa ccacgctgca cgcgtcggcg acagttgtag gatcgggaag
     7261 cgtcgggcct gatgccagcg cggattcctg gccttcgggt acatcggtca cgcccaacgt
     7321 gatcttcgtg gcatcgcctg ccgcgacagc aaggcggcca cctttaaccg cagagagatg
     7381 ttttctaacc gcattgattt catcgatgga agcgccgcag gtgacgagcg tacgattcag
     7441 ttccctgaag tcttccagcg taactgcggg atcgagcggc aactcgacga gcgcggagcc
     7501 tccccctgat aagagaaaga caaccagagt tcgttctgtt gcgcgggcca gtaaatcgag
     7561 aatcgcgcgt ccagcggaga aacttcctat gttcgggata ggatgcccag cgacaatcga
     7621 gcgaaagccg tcgggcaagt tcgacggtgc cgtcggccca actacaattc cttccgctcg
     7681 aaaattcggt gaaagcgtct cgctaagtcc atgagccatt gccaaagaag ctttgccaat
     7741 ggcaatcgca agaatctgct cgtacgccgc cagatcgtag ggcgcgccgt ttacgaaaat
     7801 ctgcgagccc gcgcggcaaa gttggcgctg catggaagca ggaatatcaa ttcccgccag
     7861 agtctcgcga aagatttgga gcgcgcattg tttaaggtcg gccattggtt cgggggttga
     7921 gtagcacagg cttcagcctg tggccttcca ttctggcatt taaaaagcga aattaaagct
     7981 atgcactcgc gcaaaaaatc cacaggccga agcctgtgct acatgaaact atccttttac
     8041 gaaggcggcc acgcctgctc gtgctacttc cgtgtcttcg tcttcggagc cggcgcttac
     8101 gccaatcgct cctaccacct ggccactcac ttcgagaatc aggcctcccc ggatcggcgt
     8161 ttggtgcgcg cggcttccaa tcgccagccc cagcgacaga agcacgctga cctcatcgcc
     8221 actgcgcgcg ggaccggtgt tctggcgacg aatcgcagcg gcatgcgctt tattaaatgc
     8281 aatgtcgatc gatgcaggtt ttgcgccgtc catgcgcgcg aacgccatta aaaatccgcc
     8341 atcatccaca acggcaatat tcatcggcac tcctatgcga gccgcttgtg ctttggccgc
     8401 gtctagaatt gcttccgcgc cttccagcgt aagttttgga tgattcagcg atacgtgtgc
     8461 catctttccc tcccggaata agtaatctca agtctatcgc ggagcaaagc tcttttctcc
     8521 tgccactcag tgatttgaag ttttaagcaa cgcttctgga ttcaaaatcg tgggtggacg
     8581 ctgtccggcg aacagggcaa ttgcattttc cacggcaatc atcgccattt ttgtgcgcgt
     8641 ttcaatggac gctgacccaa gatgcggagc gaggaccacg ttgtcgcggc gcaaaccatc
     8701 gggaatgata ggttcgcgct cgaaaacatc gagtgccgct ccggcaataa ggccgcgttc
     8761 cagcgcctcg ataagcgccg cctcctgtac aacgggaccg cgcgttgtat tgatcagaaa
     8821 tgccgtcgcc ttcatcttgg caagttcctg gggaccaatc aaaccgcgcg ttgcaggatt
     8881 cagcggaaca tgaagcgaaa caaaatcggc ttgctcgaat acagcgtctc gcgacacgta
     8941 ttcagcattc agttcctttt caatttccaa cggcgcgcgg gatgtgctgc tgtaaatcac
     9001 gcgcatgcgg aatcctaaag cgcggcgtgt cacggcgcgc ccgatgcgtc cgaacccgat
     9061 aatgccaagc gtcttgcccc aaatgtcagt cccgcacaat tgatcgagat tccacttctt
     9121 ccatgcgccg gaccgcgcca tgtggtcggc ttcaatcagg cgtcgcgcaa cggccatcag
     9181 caaagtccat gtgaaatcgg ccgtcgtttc atccagcacg ccgggcgtat tggtgacggc
     9241 gacattacgt tttgtacacg ccaggacatc aatattgtcg tagccaaccg cgacattcgc
     9301 aacaatccgc aagttgggcc cggcaatctc gagcatttcg tcatcaattt tttctgtaag
     9361 caggcatatg agggcgtcct tgcctgctac acgcttgagg aattcttccc gggacgggcg
     9421 ttcgtctccc ttccaatatt cgacttcaaa atattcctca agtttttttc gtggtccttc
     9481 aaacaaaaga tgcgtggaaa gtagtttcgg cttgctcaca cgaggcctcg acggttgata
     9541 aacaactggg agagagtatc acaagcgatg cgactgctgc gcggttgccc gccacccaaa
     9601 aaggttacgg caaaaaatta aaataattga ggacagttta atgaaaatca gccaattgga
     9661 aaattatcca ggaagttcga tcacgcgttt cggagttccg cgacggcgtg gtccataaat
     9721 cagtgtcacg gttgcgccgc gcatcaaagg agacgcttgc agaacaggag caattgcctt
     9781 gtagatggct tcggcatccg ggccgtacac aaaatatctg ccttcacttc cgtcggctgg
     9841 aagcgcatgc ccgtcatact ccccaacttt agcttcggca atcgcttgct gcagcacatc
     9901 tccaaacgaa taaacgtgct gaaaattagt ggacccgtac gcgaaatgta tctccacttc
     9961 ctgcacgggc ggtccatcat tttccgaatt tttcgagccg aataattttg agaataggcc
    10021 catgaatact catctccaat tacatgtcag attgccactt tgtacgcatt aagctaagag
    10081 atgattcttc cccaaagttg gtcaaacagc tggccgtcgt tgcttgacgg accaccgcac
    10141 cggattttac cctcatccac aaacttttcg cgacattttc tcatgactcg aattagagtt
    10201 aactcacaac ttgtttgttt ttaattaatt acaagcgtgc ctgcagcccg cgttgacctt
    10261 cccaaatgca cagactatag tgacaaagag ccttacaaaa tattcccatg caacagcaac
    10321 tcattcgcga acttgaagcg ctgcttggtc ccaaggccgt attgtcctcc gccgaagatt
    10381 tgatgctgta tgaatacgat gggtcggtgg agttgggttc tccgcgctgc attgtttttc
    10441 ccagctcagc gcgcgacgta tccggaatcg tgaagcttgc gaatcgttac gaaactccca
    10501 tcgtcggtcg tggcgcgggc acagggcttt cgggcggcgc cattgcgcgc agcggcggta
    10561 ttgtaatcgc gttctcgcgc atgaaccgca ttcttgaaat tgacggggca aatcttcgcg
    10621 ccgtcgttca acccggtgtc gtgaatctgg atttgtctcg cgcggtcgag cacctcggct
    10681 tgtttttcgc gcccgatcct tcgagccaaa aggcctgcac gattggcggc aatgtctccg
    10741 aaaattccgg cgggcctcat actttggctt acggcgtgac gaccaaccac gtgactggca
    10801 tcgaactcgt attgaccgac ggcgaaattg tgcgcatcgg cggtaaaaac attgagtcgg
    10861 ctgggtatga tctgggtggg ctgctggttg gctccgaagg aactctcgcg ctcgtcacgg
    10921 aaattacggt ccgattgatg cgcaagcccg aggccgtgaa aacgctgctc tcggtgtatg
    10981 acacgctgga cgacactacg gaatcggtcg tggacatcac ggtgcgcgcg attactccgg
    11041 ctgcctgcga aatgctggac ggctttacgc ttcgcgccgt ggaggattac gttcacgcgg
    11101 ggttccccaa agactgcgcg gctgttctgc tgatcgaagt ggaagggctt aaagaagccg
    11161 tagaagcgca ggccgccgag attaccgaag tctgccgcga acatcgcgca agagaagtgc
    11221 gagcggcgcg caccgatcag gaacgcaatc tattgtggag gggccgaaag gaagcgtttg
    11281 gcgcgatggg gcgtatttcg cccatgtatt acgtgcagga cggcgtgatt ccccggacac
    11341 gacttcccga aactttgcga cgcatcgagg aaatcagcaa gaagtacgat ttgcagattg
    11401 gcaacatttt tcacgcgggc gacggcaact tgcatccgct gattttgttc gatgagcgca
    11461 atgccgcgca atttgcccga gtggtcgcgg ccggcggcga gatcatgagg cattgcgtgg
    11521 aagttggcgg tgcgctcacc ggagaacacg gcataggc
//
LOCUS       DASZMB010000023        14558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1402061, whole genome shotgun sequence.
ACCESSION   DASZMB010000023 DASZMB010000000
VERSION     DASZMB010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 14558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14558
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1402061"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(415..987)
                     /locus_tag="VG272_00920"
     CDS             complement(415..987)
                     /locus_tag="VG272_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12268.1"
                     /translation="MPGSAANGEVLSNTLSIKVADASTIARKWGPQVKNLAVDVTLDK
                     NTYAVGEDVALHIAMENFGADRHIYGPSLIWHPCQIVTIQLFDSTSGENRKAAFECRD
                     SGPSSSYFFEYVTGKVIPLEFTLREVGLLPDKPGTYSVVVTWAPFGSKVVCLGAIPIE
                     PVCQFHAPYATVHSASGSFRILEPAFKNSH"
     gene            2009..3130
                     /locus_tag="VG272_00925"
     CDS             2009..3130
                     /locus_tag="VG272_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12269.1"
                     /translation="MKSIRVLLSILLLAPLSVPARADFKYTETAKITGGALKGAMKFA
                     GVFSKQASEATKPMVTTHYVQGKRLRTDNPDGKIQIIDLEGRRIIDIDTQKHTYSEIT
                     FDQMKEAMQNAQQKVQQKMDQDPKAKDAKANVDVKFKVTPGTGTRQILGKNTNETKVQ
                     IEMDMQAQDSSQPSQPSGPVSGTIVTTMDMYVAPSVPGYEELGQFYVQMAKEINWVPP
                     SNIHVDPRASQSLDELQKNSGNLKGFPLLQYMTMSMAAQDSSGNTVQTSNTSASSSSS
                     SASSSSNSDSTPTSISGAMTKGLGGLFSKKKKQDDTADQNSQNPPPPSTPGSLMEMTI
                     EVNSYSDSSLDSSLFDIPAGYSRITQNADQIMGATPAKQ"
     gene            complement(3224..6994)
                     /locus_tag="VG272_00930"
     CDS             complement(3224..6994)
                     /locus_tag="VG272_00930"
                     /inference="COORDINATES: protein motif:HMM:NF019912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12270.1"
                     /translation="MGKRNHFARMDRIAPWAALIISMALALLAARISAPSLHAADEKS
                     GPNLMSQTQEEADRKSAGCISCHTPTDEPTMHATGTVRIGCTDCHGGNADVHVASDIA
                     ATSAEYEQAKRKAHPQPRNPKLADRSSNAEWIYTEWLKESPEYVRFVNPGDLRVAPET
                     CGRSGCHTSETRNVSTSMMTTGGMLWGAALYNNGGYPHKDTRFGESYSRDGLPQTVLT
                     IPQPSAEETRTKGILPEITPLERWEISQPGNILRVFERGGGPKGEVGEPLRGDDAGKP
                     DDKLSARGFGTLLRTDPVFLGLQKTRLLDPLLSLPGTNDQPGDYRASGCTACHVVYAN
                     DRSAQHSAQFSTYGNRGYSATKDPTIPREESGHPIRHEFTRSIPSSQCMTCHVHPGTN
                     MMTTYFGYTWWDNESDGGAMYPKAQHNPTQEEQFAVSQRNPEGAAARGLWSDVKFLAE
                     TGSAAFNAKLKDTQFADFHSHGWLFRAVFARDREGRLLDEKGAAVSADAPNKFDKAAH
                     LADIHLQKGMQCVDCHLEQDSHGTGKLYGEPRAAIEMDCVDCHGTISQPATLVTSGPA
                     ASGSGGTHLDALRTPWRERQFEWRDGKLIQRSMMDPHREWEVVQTLDSITPGNAHYSE
                     KSRLAKTMRKNGVTWGDAPKDEAQLAHANSSMTCYSCHTSWTPNCFGCHLSMTANEKR
                     PMLHNEGLTTRNWTSYNFQVLRDDGYMLAVDGTVTGHRIAPARSSCAILVSSQNTNRE
                     WLYNTQQTVSAEGFSGQAFSTFVPHTVRARETKQCSDCHVSAARDNNAWMSMLLLQGT
                     NFLNFMGRYVYVATGKRGFQAVVTAEHDEPEAVIGSDLQRIAYPDDFRKHEERKRELK
                     TAYGHDGNVLDIQLRGEYAYAAMGADGFRVFDVANIDNKGISERMVTAPVSPLGQRLY
                     VKTKFATAVATPTTLAVDPLRTHDPANEEQKIHLMYGFLYVTDKYEGLIVIGNPNLKG
                     KNPGVGTLLDGNPENNFVTRAATFNPGGILNGARRITIAGTYAYILCDRGLVVVDLDN
                     PLAPRVTAEIGAPDLVEPQGVAVQFRYGFVADREGLKTLDVTDLAHPRIVPGAFVKLD
                     DSRNVTVSRTYAYVSAGKNGIALVNVTQPEKPRVDRMFTGGGALNDVNDVKIGAVDAS
                     GFAYVADGRNGLRILQVISPEDSTESYGFSPRPTPKLIATYRTSGPALAVSRGIDRDR
                     AVDESGNQLAVFGRRGARPFNRAEAEKLFLRDGKLYTVTDTPPGPPLLPAGK"
     gene            complement(7054..9093)
                     /locus_tag="VG272_00935"
     CDS             complement(7054..9093)
                     /locus_tag="VG272_00935"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWF12271.1"
                     /translation="MRLPRQAARKTAALQGVVTDSAGRGIIGAVVAITNRSTGMAWTV
                     STDADGVFRWTELAPGNYLLLVQNEEFESVTRDNLTLDAGDGVTLELTLSPSTISTTI
                     NSRLPRMPELGSPAAMASAVGSFAPYGGIRRRPDAGTMQEIPAPETLPPSQQVFLDAR
                     DRWNIAMPDWNRYGRGGEYPYVRANHWWDPFNRNRLKGDLPIWGQQTFLNITATSDTF
                     VDERRVPSTSNISSARPGSSDYFGKGEQFALNQTFRFSFDLFHGDTSFRPVDWRIQLT
                     PAVNVNYLDVRELGIVNIDVAKGTTRLEAHAGLQEAFAEYKIHDLSTNYDFISVRAGI
                     QGFSSDFRGFIFVDEQPGVRVFGNLHSDRWEYNAAYFNLLEKDTNSGLNTFQPRHQQV
                     IVANFYMQDFVKPGYTTEFSIHYNKDDASVHYDDNGFLARPAPVGVFQPHEIRAAYLG
                     WTGNGHFGRININHAFYEALGADNLNPIAGRPVTINAQMGAAEVSLDKDWVRYKVSAF
                     YASGDGNPRDGRATGFDTIVDDPSFAGGFFSFWDREELRLPGTGVALTQGNSLLPSMR
                     TDKEEGQANFVNPGIFLVNAGANFDLTPKLKTFLNVNYLRFEHTAPIALLLFESPIHN
                     TIGVDTSIGFQYRPPLSENISITGGAAALFPGQGFRDIFSGTTQFSIFADVHFQF"
     gene            9617..10300
                     /locus_tag="VG272_00940"
     CDS             9617..10300
                     /locus_tag="VG272_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HWF12272.1"
                     /translation="MSNARPTPVLAFDVYGTLIDPLQMEEHLRPAFGEKAREASELWR
                     SKQIEYSFRRGLMKKYAPFDVCTAQALRYVSTQLGISISEEAHARLMTKYLELPAYPD
                     VTSTLDELAAQGFVITACSNGTENAVRTSLDHAGILSRFSKIVSVDPLRTFKPDPAVY
                     EYLVAELHARRDLVWLISSNPFDVIGARACGLRTAWVQRDSKRVFDPWEFEPDLTVHG
                     LAELPGELK"
     gene            10466..12049
                     /locus_tag="VG272_00945"
     CDS             10466..12049
                     /locus_tag="VG272_00945"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HWF12273.1"
                     /translation="MITKQRPLMGLLIDRIKKTRPLQKLFLVLTAALAIGIAGCGGVA
                     GFLTSAPPPSSVQSLTAADVSTLVQTAAQAADPNTMVIAVVDRSGNVLGVYRKPSAPT
                     LVTGNFNAQVDANELAVSLARTAAFFSNDQAPLSSRTVRFISGVHFPPGVADTNNAPL
                     YGIENTNRGCTLSTNFMAGQSLPPARSISGATTGLGIATGKVDVNDSDESAVNPGGVP
                     LFRNGSLVGGIGVTGVAPSVAEFAAYTAATSNGFGPTPAAPGVVFIGGVALPFVNQTT
                     RPSGVNAGTFTGSFVAGPTASSATPPEGMLVASVAGPLGGLAASDVTQIINNAIATAN
                     KTRAVIRLPIGSSARMAFAVADLDGTIIGLYRMADSTVFSIDVAANKARNMVYFNSAS
                     RQASDLTGVPFGTAVTNRTIGYGAQPFFPPGINGSNAGPFFNLYVQDVANPCTQGFQV
                     AGPNQSGIVFFPGSVPLYRNGVLVGGLGVSGDGVDQDDYVASGGSVGFEAPLAIRADQ
                     VIVQGVRLPYLKFPSNPTN"
     gene            complement(12425..>14558)
                     /gene="priA"
                     /locus_tag="VG272_00950"
     CDS             complement(12425..>14558)
                     /gene="priA"
                     /locus_tag="VG272_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HWF12274.1"
                     /translation="EREFLLTSAGRIHLAQLHSLGNRSEAEVAEMALLSLMEIEGRPV
                     RADRLHKLPGGEAAAERLLRRKQLESHEVAVHRKTRVQKIVAWNTAVSSEVTPSADPS
                     AALPEKEARVFHVLAEERGPLPFPHLAKLAKVSRPLIEKMIHQEKLKCWEEPIAIEED
                     LFDVDYTPPSNILNVDQERELAVIRGWLGAGVFTAGLLYGVTGSGKTEVYLNAVEEVL
                     ARNKTALILVPEIALTLWVGRLCRAKFGAGVAVLHSALAEVERAREWWRVRRGEARVV
                     VGTRSAVFAPVENLGLIVVDEEQEASYKQEETPRYHGRDVAVVRAKLEGAVVLLGSAT
                     PSLESFQNARSGKYQLLQLDSRVENRPMAPVEIVDLREDFKATHNTGPISSKLNKAMG
                     ARLTEGTQSLILINRRGYSWSVICRSCGISVQCENCSISLTYHKQRHRLECHYCGFSR
                     RVPDVCPKCNSEHVYFFGAGAEQLEEKLREKFPEARIARLDRDSVRTKSAYQQVLGDF
                     ATGKIDILVGTQMVAKGHDFQGVTLVGVISADSQLSLPDFRAAERTFQLLTQVAGRTG
                     RGALPGEVLVETYYPEHYAIQLAARQDYLTFFEKEVQFRRLLHYPPFTALASILVRDT
                     KIENAIRWSRQLSAFLAPMESRGVKVLGPAAAPLARLKREYRFQFLLKAPKRAQVTRA
                     VSELLAFSEEKEIPQKAVLVDIDPLSLF"
BASE COUNT         3281 a         4082 c         3871 g         3324 t
ORIGIN      
        1 gcattattcc ttcttctacg agacgagcat cagacccgta ttgctcaagt aaattgcgcc
       61 cacaccatgg acagaatttt aattttatcc catcttcggc tatgggttct ggtggcattc
      121 gggtcggacg acgatattcc aataggaaaa ccggagcgga tctggaacgg acgagccacg
      181 aaatcccgag tccgtcccgt cttccctccg tttgaaagaa tcgctcctga aactccgggc
      241 agcaccattc ggatttcata aaagaaatcc tatttccaag tcggtccaaa gtaaaggaac
      301 cggcgattcc cagctagcga taaccccgtt tttcaggact tggcggtcca gcaaccgcga
      361 ccggcggggt caagtgtgtt acgcgggttt cgatacagtc ggacagcaaa tacgttaatg
      421 gctattcttg aatgcgggtt cgaggatgcg gaaactgccg gacgcggaat ggacggtcgc
      481 gtatggggca tggaattgac aaactggttc gatggggatc gcaccaaggc agactacctt
      541 acttccgaag ggcgcccacg tcaccacaac ggaataggtc ccaggcttgt ctggcaacaa
      601 ccccacttct ctgagagtaa attcaagggg aatgacctta cccgttacat attcgaaaaa
      661 gtacgatgac gaagggccgc tgtccctaca ttcaaaggcc gccttgcggt tctctccact
      721 agtcgagtca aacaattgaa tcgtgacgat ctggcatggg tgccagataa gactcgggcc
      781 gtaaatgtgg cggtccgcgc cgaaattctc catcgcaatg tgtaatgcga cgtcttcgcc
      841 gacggcatag gtgttcttgt ccagcgtcac atcgacggcc agattcttaa cctgaggtcc
      901 ccatttccgt gcaatcgtcg acgcgtcggc gactttgatg gaaagtgtgt tcgacagaac
      961 ttcaccgttg gcagcggatc cgggcatctg gacaatacta aaatcatgtt ctccaattcc
     1021 gccccacctt gatcgtgcgc ccacaatatc gaaacccgtg tgccaatccc ctggcgggaa
     1081 ccctgggaca tacgatggac agcccttaaa tgcagcagga gccactttgt caacgcgact
     1141 agacccgtca ggggatcgtt ggcgcagaaa aaatgtggga caggtggttt cgtctgctgg
     1201 tatccccggt tgagccgcag aaacgtgcaa atagaagtgc tcgccttcat agtaggtgtc
     1261 ccggtccgat gacagggtta agggatgccc atctgcctgc acagattggg gagacttttg
     1321 ggcttggact cggagtgaaa aagccgccaa gcaagataga gcaataagac acgctcttgc
     1381 gattgatgag gaagttggca tgcgattcag tttcgcactc ttgcgccgtc ggcaagaact
     1441 cgaacgtatt atgtcacatt caggtttcca cggcatgtcg ctgccaagtc ccgcgaagtc
     1501 gtgccagctc aaccggtcga cgcaacacct tataagcagt tgatctgaca atacttccca
     1561 accagaatta atccgtacgg cagtacgtga tcagaaccta tacaggactt tcgttttgtt
     1621 tgatgtgaag cctacgaata aaatcggctt ctggactctc ataacctgac cttgagcaag
     1681 tgttgcatcg acccattgag accgcccgtc ttctcggaat gtgggttttg agcctctgtc
     1741 acattccaag atttctatct ttaagcttgg cttggcagta gaggaagggc cgcattatca
     1801 gaagaaattt taagcgaaat atcaggggaa atagattcag atgtttcctc caatccactt
     1861 cgcggtatga tgctggcaac tgtaggataa aaaccgtcgg ccagagtacg cgctaaacct
     1921 caggtcaccc aaccggctag gggatagagt tattgcaatg aggcatgccc cgagtttata
     1981 cattcattct gcttacggag gtaacgtcat gaagtcaatt cgcgttctat tgtccatctt
     2041 gctgctggcg cctttgagcg tcccggctcg agctgatttc aaatacacgg agacagctaa
     2101 aatcacaggc ggcgcgttga agggcgcgat gaaattcgcc ggcgtattca gcaagcaggc
     2161 atcggaagca acgaagccca tggtcactac gcattacgtc caaggtaagc gtctccgaac
     2221 agataacccc gacggtaaga tccaaattat cgatctcgaa ggacgccgca tcatcgatat
     2281 cgatacacag aagcacacgt attccgaaat tacatttgac cagatgaaag aggcgatgca
     2341 gaacgcgcag cagaaagtcc agcagaaaat ggaccaggac ccgaaagcca aagatgcgaa
     2401 agccaacgtc gatgtgaaat tcaaggtgac tcctggaacc ggaacccgcc aaatattggg
     2461 gaagaacacg aatgaaacga aggtccaaat cgaaatggac atgcaggcgc aggacagcag
     2521 ccagccgagc cagccctccg ggcccgttag cggaactatc gtgacgacga tggacatgta
     2581 tgtcgcccca tccgttcctg gatatgagga gctagggcag ttctatgtgc agatggcaaa
     2641 ggaaatcaat tgggttccgc cctccaatat tcacgttgat ccacgggcat cacaaagcct
     2701 tgacgaactc cagaagaact caggcaatct gaaaggtttt cctcttctcc aatacatgac
     2761 catgtccatg gccgcgcagg attcgtctgg aaatactgtg cagacctcga atacttcggc
     2821 ttcatcgtcc tcttcttccg cctcttcttc ctcaaactcg gacagcacgc ccacaagtat
     2881 tagcggcgcc atgacaaaag gcctcggcgg attattctca aagaaaaaaa agcaggatga
     2941 tactgccgat cagaacagcc agaatcctcc tccgccatcc actccaggtt ccttgatgga
     3001 gatgaccatc gaagtcaatt cgtattcgga ctcgtcgctg gattccagtt tgtttgatat
     3061 tcccgcgggt tattcgcgca ttacgcagaa cgccgatcag atcatgggcg ctactccagc
     3121 aaagcagtag ataaaaacgg cgttgagtgc agtacgaaaa gtgccctaca aattaaagaa
     3181 ggacccggtg atttcaggaa atatgccatg gaggagaagg tttctatttc cccgctggca
     3241 agagtggcgg tcccggcggc gtatctgtca cggtgtaaag tttgccgtcg cgcaaaaata
     3301 atttttcagc ctccgcgcga ttgaatggcc gtgcgccgcg ccgtccaaaa acagcaagct
     3361 ggttgccgct ctcgtcaacg gcgcgatctc gatcaatgcc gcgcgaaacc gcaagtgcgg
     3421 gaccgctggt tcgatacgtc gcaattaatt tcggagtagg ccgcggactg aatccgtacg
     3481 attccgttga atcctcgggt gaaatcactt gcaggatgcg caagccatta cgcccatccg
     3541 ccacataagc gaacccgctg gcatccaccg ctccaatctt cacgtcattc acatcattca
     3601 gtgcgccgcc acctgtaaac atccggtcaa cgcgaggctt ttccggttgt gttacgttga
     3661 cgagcgcgat cccgttcttg cccgctgaaa cataggcgta agtccgcgac acggtgacat
     3721 tgcgcgagtc atccaatttt acaaatgctc ccggcacgat gcgcggatgc gcgaggtcgg
     3781 ttacgtcgag cgtttttaat ccttcgcgat cggcaacgaa gccataacgg aattgtacgg
     3841 ccacgccttg cggttcgacc agatcgggcg cgccaatttc cgccgtcacg cgcggcgcaa
     3901 gcggattatc gagatccacg acgacgagcc cgcggtcgca caagatatac gcgtaggttc
     3961 cggcaatcgt aatgcgtcgc gctccgttca aaattccgcc aggattaaat gttgccgcgc
     4021 gcgtaacgaa attattttcc ggatttccgt ccagcaacgt acccacgccg ggattttttc
     4081 ctttgagatt tggattgcca atgacgatca gcccttcgta tttatccgtg acgtaaagaa
     4141 atccgtacat caaatgaatt ttctgctctt cgtttgcagg atcgtgtgtg cgcaacggat
     4201 cgacggccag cgttgttgga gttgcgaccg ctgtcgcaaa tttcgttttc acatacaggc
     4261 gctggccgag cggagaaacc ggcgccgtca ccatgcgttc ggaaataccc ttgttatcga
     4321 tgttggcgac gtcgaaaacg cggaacccgt ccgcgcccat cgccgcgtag gcgtattctc
     4381 cgcgcaattg aatatcgaga acatttccgt cgtgaccata agcagtcttg agctcgcgct
     4441 tgcgttcttc gtgtttacgg aaatcgtctg gataagctat gcgttgcaaa tcactgccga
     4501 tcacggcctc aggctcatca tgttccgcgg tcacaacggc ttgaaagccc cttttgcctg
     4561 tggccacata aacgtagcgc cccatgaaat tgagaaaatt cgtgccttgc aacaggagca
     4621 tcgacatcca cgcattgtta tcgcgcgcgg cggagacatg acaatcggag cactgttttg
     4681 tttcgcgcgc gcgcaccgtg tgtggcacga acgtggaaaa tgcttgcccg ctgaaaccct
     4741 ctgcggaaac tgtttgctgc gtgttgtaca gccactcgcg attcgtgttt tgcgaactca
     4801 caagaatcgc gcacgaagat cgcgccggcg caatgcgatg tccggtaaca gtgccatcca
     4861 ccgcaagcat gtagccgtca tcgcgcagca cttgaaaatt gtaggatgtc caattgcgag
     4921 tcgtgagccc ttcgttgtgc agcatcggcc gcttttcgtt cgcggtcatc gagaggtggc
     4981 agccaaaaca gttgggcgtc catgaagtgt ggcacgagta gcacgtcatg ctgctgttcg
     5041 cgtgcgcgag ttgcgcttcg tccttcggcg cgtcgcccca cgtgacgccg ttcttgcgca
     5101 tcgttttcgc gaggcgcgat ttctcgctgt aatgcgcgtt tcccggcgtg atcgaatcga
     5161 gcgtttgcac cacttcccat tcgcgatgag ggtccatcat cgagcgttga attaattttc
     5221 cgtcgcgcca ctcaaattgc cgctcgcgcc acggtgtgcg cagtgcatcg aggtgcgtgc
     5281 cgccactgcc ggatgccgcc ggccccgaag taacgagcgt tgcgggctgc gaaatggttc
     5341 cgtggcaatc gacgcaatcc atctcaatcg cggctcgcgg ctcgccgtaa agttttcccg
     5401 tgccgtggct gtcctgctcc aggtggcaat ccacgcactg catgcctttt tgcaagtgaa
     5461 tgtcggcaag gtgagcggct ttatcgaatt tgttcggagc atccgcagaa accgccgcac
     5521 ctttttcatc gagcaagcgg ccttcacgat ctcgcgcaaa cacagcgcgg aagagccatc
     5581 cgtggctgtg aaaatccgcg aactgcgtgt cttttagttt tgcgttgaac gcggcgctgc
     5641 ccgtctccgc caaaaatttc acgtcactcc acagcccacg cgccgccgcg ccttcgggat
     5701 tgcgctgcga cactgcgaat tgttcttcct gagtgggatt gtgctgagcc ttcggataca
     5761 ttgcgccgcc gtcggattcg ttgtcccacc acgtgtagcc gaagtacgtt gtcatcatgt
     5821 tcgtgcccgg atgcacatgg cacgtcatgc attggctcga cggaatggag cgcgtgaatt
     5881 catgccggat cggatggccg gactcttcgc gtggaatcgt cggatctttc gtcgcgctgt
     5941 atccgcgatt tccatacgta gaaaactgtg cggaatgctg ggccgaacgg tcattcgcgt
     6001 ataccacatg gcatgccgtg catccgctgg cgcgataatc gcccggctga tcgtttgtgc
     6061 ccggcaagga aagcagcgga tcgagcagtc gcgttttctg caatccaaga aatactggat
     6121 cggtgcgcag tagcgtgcca aatccacggg cgctgagttt gtcgtcgggt tttccagcgt
     6181 catcaccacg cagcggttcg ccgacttcgc ctttcgggcc gccgccgcgt tcgaatacgc
     6241 gcagaatgtt tcccggttgc gaaatttccc agcgttccaa gggagtgatc tcgggcaata
     6301 tccccttagt ccgagtttct tccgccgagg gttgcggaat tgtcagaaca gtttgcggta
     6361 agccatcgcg cgaatagctt tcgccgaatc gcgtgtcttt gtgcgggtaa ccgccattgt
     6421 tgtacagcgc agcaccccag agcattcccc cggtcgtcat catgctcgtg gatacgttgc
     6481 gcgtttcgga agtgtggcat ccgctacggc cgcacgtttc aggagcgacg cgcaaatcgc
     6541 ccgggttcac gaagcgcaca tactcggggc tctccttaag ccactctgtg tagatccatt
     6601 cggcattcga tgatcgatcc gcgagtttcg gattacgcgg ttgcgggtga gcttttcttt
     6661 ttgcttgttc gtattcagcc gatgtggcgg cgatgtccga agccacgtgc acatccgcat
     6721 ttcctccgtg gcaatcggtg cagccgattc gcacggtacc ggttgcgtgc atcgtcggct
     6781 catcggttgg cgtgtggcag gaaatgcatc cggcgctttt tcggtcggct tcttcctgcg
     6841 tctggctcat caagttcggg ccggactttt catcggccgc gtgcaaggag ggcgcggaaa
     6901 ttcgcgcggc aagcaaagca agagccatgc taatgataag cgccgcccag ggggcgatgc
     6961 gatccatgcg cgcaaagtgg ttgcgtttac ccattccgcc ttaaattcct tcttccaaaa
     7021 tcaaatgctt cgacgcgcaa attttaattc gcatcaaaat tgaaaatgca catctgcaaa
     7081 tatggaaaac tgagttgtgc cggaaaaaat atcgcgaaat ccttgtcctg gaaacaaggc
     7141 ggcggcgcca cccgtaatag aaatgttttc ggaaagcggc gggcggtatt gaaagccgat
     7201 gctggtgtcc acgccgattg tattgtgaat gggcgattca aaaagtaata gggcaatggg
     7261 agcagtatgc tcgaaccgca aataatttac gttcaggaag gttttcagtt tcggcgtcaa
     7321 atcaaagttc gcacccgcgt ttaccaaaaa gatcccaggg ttcacgaagt tcgcttgccc
     7381 ctcttcctta tcggtgcgca tgctgggcag caaactattc ccctgagtta gcgctacgcc
     7441 tgtcccgggc aagcgcaatt cttcgcggtc ccagaagctg aaaaatcctc ccgcaaaaga
     7501 tggatcgtcg acaatcgtgt caaaaccagt tgcgcggcca tcgcgtggat ttccatcgcc
     7561 cgaggcatag aacgcggaaa ctttatatct cacccaatct ttatccagag aaacttccgc
     7621 agcgcccatc tgcgcgttga tcgtcacggg acgccccgca atcggattca ggttgtcggc
     7681 gcccagcgcc tcataaaacg cgtgatttat gttgatgcgg ccaaagtggc cattgccggt
     7741 ccagccaaga taagcggcgc gaatttcgtg aggctgaaaa actccgacgg gcgccggacg
     7801 cgccaaaaaa ccgttgtcgt cgtaatgcac gctggcatcg tctttgttgt aatgaatgct
     7861 gaactcggtt gtgtagccgg gcttcacaaa atcttgcata taaaagtttg cgacaatcac
     7921 ctgctggtgg cgcggctgga aagtattcag gccgctgttg gtatcttttt ccagcaaatt
     7981 aaaataggcc gcgttatatt cccagcgatc cgagtgaaga tttccgaaca ctcgcacacc
     8041 cggctgctca tcgacaaaaa taaatccgcg gaaatcactg gagaaacctt gaatccccgc
     8101 gcgcacggaa atgaaatcgt agttcgtgct taaatcgtga atcttgtatt ccgcaaacgc
     8161 ttcctgcagt ccggcatgcg cctcaagacg cgtcgtgccc ttggccacgt caatattcac
     8221 gattccaagt tcgcgcacgt ccaggtaatt gacattcacc gctggtgtca attgaatgcg
     8281 ccagtcgacc gggcggaacg aagtgtctcc gtgaaacaga tcgaacgaaa atcggaatgt
     8341 ctgattgagg gcgaattgct cacccttgcc gaaataatcg ctgctgcccg gacgcgcgga
     8401 actgatgttg ctcgtcgacg gcactcggcg ctcatcgaca aaagtgtccg aagtcgcggt
     8461 gatgttcaag aatgtttgct ggccccagat cggcagatcg cctttcaacc gattgcgatt
     8521 gaacgggtcc caccagtggt tcgcgcgaac gtaaggatat tctccgccgc gtccgtaacg
     8581 attccaatcc ggcatcgcga tattccaacg atcccgcgca tccagaaaaa cttgctgcga
     8641 cggcggcagc gtttccggtg cgggaatttc ctgcatagtg ccagcgtctg gtcgacggcg
     8701 aatgccgcca taaggagcaa atgaacctac tgctgacgcc attgccgctg gcgagccgag
     8761 ttcaggcatg cgcggcaagc gggaattgat cgtcgtagag atcgtcgaag gagaaagcgt
     8821 cagttcaagc gtcaccccat ctccagcatc gagcgtcaaa ttatctcgcg tcacactttc
     8881 aaactcttcg ttctgaacca acaataaata atttcccggc gcgagttcgg tccaccggaa
     8941 cacgccgtct gcatcggttg aaactgtcca cgccattcca gtcgagcgat tcgtgatagc
     9001 aacaactgcg ccgataattc cgcgtcccgc tgaatctgtt accacccctt gcagcgcagc
     9061 agtcttgcgc gcagcttgcc gaggcagccg caattcatct tggttttgaa ccgagtcgtc
     9121 tggaaatgcg ggaagtggcg acgcatcatc taacgccgag aaatttcgcg tcgggatacg
     9181 cacggagacg agttcaaact tagattgggt gtgtgagcga attaaatatc ttttgtgcgc
     9241 ggtacgtcgt gaatgagcgc ccttcgcgct tgcgcccttg tggtgttgcc gcgccgatgc
     9301 gtgtttggat gatgaagata atgttgcagg ctttgccacg gtcaccggca ggaatgccag
     9361 cacggagcca aggcaaagac ttcgaattgt ttcggatgcc gcccgcgtcc agattgtttt
     9421 aattctcctg ccagcaaaaa attgccaatc ccaccgtgga ctagctaaca acgatctcag
     9481 ggtcgcggca aggtgttttg ccccggcatg cgccaagtta gaaacactcc ctttgcaata
     9541 aaaaaacttg aaaaaattca ctttgcggtt tctctgcgga ttcaaaaagc ctatgctgtt
     9601 cggacgagga atatttatgt caaatgcgcg tcccactcct gtgctggctt tcgatgtcta
     9661 cggcacgctt attgatccat tgcaaatgga agaacactta cgccctgctt tcggggaaaa
     9721 ggccagggaa gcatccgaac tttggcgtag caagcaaatt gaatattcat ttcgtcgtgg
     9781 cctcatgaag aaatatgctc cgttcgatgt gtgcacggcg caggcgcttc gatatgtcag
     9841 cacgcaattg gggatttcca tttctgaaga agcacacgcg cggttgatga cgaaatatct
     9901 ggagttgccc gcatatccgg atgtgaccag cactttggat gaactcgcgg ctcaaggttt
     9961 tgtcatcaca gcgtgttcca acggaaccga aaacgcggtt cgcacatcgc tcgaccatgc
    10021 gggtatcctt tcgcgcttca gcaaaatcgt gagcgtggac ccgctccgca cttttaagcc
    10081 ggacccggcc gtttacgaat atctagtggc tgaattgcac gctcgaagag atttggtctg
    10141 gctcatttcg agcaaccctt tcgatgtaat cggcgcgagg gcctgtggct tgcgcaccgc
    10201 ctgggtgcag cgcgattcaa aacgtgtgtt cgatccttgg gaattcgagc cggacttaac
    10261 cgttcatggc ctcgctgaat tgccgggcga actgaaataa ttgcgttaga ttttgcgatg
    10321 gctttgcgtg gcataggctt cagcctgtgg ggtttggtcc ttgcaaggat caaattccac
    10381 acgctgaagc ctatgccacg aaaaatccaa tccaattgca gttctcgcac aacttttttc
    10441 cgcttcttga gcgcggcgca ccgatgtgat aacaaagcaa cgacctctca tgggcctgct
    10501 catcgacaga attaaaaaaa cacgaccgtt acaaaaatta tttctcgtac tgacagccgc
    10561 actggctatc gggattgcag gatgcggcgg cgtagccggc ttcctcactt cagcgccacc
    10621 gccgtcttcc gtgcaaagcc taaccgccgc ggacgtctcc acacttgtgc aaaccgctgc
    10681 gcaggctgcc gatcccaaca caatggtcat tgccgttgtt gaccgctctg gaaatgtttt
    10741 gggcgtctat cgcaaacctt ccgcgccaac gctcgtcacg ggaaacttca acgcgcaagt
    10801 ggacgcaaac gaactcgcgg tatcgctcgc tcgcacggca gcgtttttca gcaacgatca
    10861 ggcgccactt tcttcgcgca ccgttcgttt catcagcggc gttcattttc caccgggagt
    10921 tgcggataca aataatgctc ctctctacgg aattgagaac accaatcgcg ggtgcacgct
    10981 ttcaacaaat ttcatggcag ggcagtcatt gcctcccgcc cgctccatca gcggtgcgac
    11041 caccggcttg ggaatcgcca cgggcaaagt ggatgtaaac gatagcgatg aatccgcggt
    11101 caatcccggc ggcgtgcctc tctttcgcaa cggcagtttg gtcggaggca tcggtgtaac
    11161 gggagtcgcg ccaagcgttg cagaattcgc cgcctacact gcggcgactt ccaatggatt
    11221 cggccctacg cccgcagcgc ccggcgttgt tttcattggc ggcgttgctc tgccgtttgt
    11281 gaatcagacg actcggccct ctggtgtcaa cgccggaaca ttcacgggga gttttgttgc
    11341 aggtccgact gcaagttcgg cgacgcctcc agaaggcatg ctggttgctt ccgtggcagg
    11401 tccgcttgga gggcttgccg cgtccgacgt cacgcaaatc attaacaacg cgatcgccac
    11461 ggcgaacaaa acgcgcgctg taattcgcct gccaatagga tcatccgcgc gcatggcctt
    11521 tgcagttgcc gacctcgacg gcacaatcat tggcctgtac cgcatggcag acagcacggt
    11581 tttcagcatc gacgtagccg ccaacaaagc tcgcaacatg gtgtatttca atagcgctag
    11641 tcggcaggcc tccgatttaa ccggcgtgcc tttcggcact gccgtaacga atcgcacgat
    11701 tggctatggc gcgcagccat ttttcccgcc gggaataaat ggaagcaacg caggtccatt
    11761 ttttaatctc tacgtgcaag atgtggcgaa tccctgcaca cagggatttc aagttgccgg
    11821 tccgaatcaa agcggaatag ttttttttcc gggatccgtg ccactgtacc gcaacggcgt
    11881 gctggtggga ggactcggcg tcagcggcga tggagtggat caggatgatt atgtggcgtc
    11941 gggcggatcg gtaggtttcg aagctcccct agcgattcgc gcggaccaag tgatcgtgca
    12001 gggcgtgcgc ttgccttatt tgaaattccc ctcaaatccc acgaattaat tcccgattct
    12061 aaaattgggt agcatttagt agcgcgggct tcagagtctg tgtgagaact gcagaatctt
    12121 tatgggtcgt ggctttagcc acgacatagg tcctgcaaaa tgagtgcggc ttcacagttt
    12181 gcggaaaaag tagtttattc tgtcattccg agcgaagcga ggaatctctc ttcgatttga
    12241 gtataagaaa ataaagaagg agggattcct cgcttcgctc ggaatgacaa aatgttgagt
    12301 ttttccgcag cctcttcagc ggctgaagct tcaattttgc aattctcaca cagactcttc
    12361 agcctgtgga gttatgccct tgcaaagcca aaaatccaca ggctgaagcc tatgccacaa
    12421 aaacctaaaa caaactcagc ggatcgatat ccaccaatac tgccttctgc ggaatttctt
    12481 tttcctcgct gaacgccagc aattccgaaa cggcgcgcgt aacctgcgcc cgtttcggcg
    12541 cctttagcaa aaattggaaa cggtactcgc gcttcagcct cgccagcggc gcggccgcgg
    12601 gtccgagcac tttcacaccg cgcgattcca tcggcgccaa aaacgcggag agctgccgcg
    12661 accagcgaat cgcgttttca attttcgtgt cgcgcaccaa aatgctggcc agcgcggtga
    12721 acggtggata atgcagcaag cggcggaatt gcacttcctt ttcaaaaaat gtgaggtagt
    12781 cctgccgcgc ggccagttga atcgcgtagt gctccggata gtaggtctcg acaagcactt
    12841 ccccgggcag cgcgcctcgt cccgtgcgtc ccgcgacttg cgtcaacaac tgaaacgtgc
    12901 gctccgccgc gcgaaaatcc ggaaggctca attgcgaatc cgcggaaatg acgccgacga
    12961 gcgtaacgcc ctgaaagtcg tgccccttcg caaccatttg cgtgcccacc aggatgtcga
    13021 ttttccctgt cgcaaaatct cccaggactt gttgatacgc gcttttggtg cgcacggaat
    13081 cacggtccag acgcgcaatc ctggcctcgg ggaatttctc gcgcaatttt tcttcgagtt
    13141 gctcggctcc cgcgccgaaa aaataaacgt gttcggaatt gcacttcgga caaacgtccg
    13201 gcacgcgccg tgaaaagccg cagtagtggc attcgaggcg gtggcgctgc ttgtgatacg
    13261 tgagcgaaat gctgcaattc tcgcactgca cgctgattcc acaggaccgg caaattacgg
    13321 accacgagta gcctcggcga ttaatcagga tcagcgattg cgtcccctcg gtcagtcgcg
    13381 cgcccatggc tttatttagt tttgaggaaa tcgggcccgt gttatgcgtg gccttgaaat
    13441 cctcgcgcag gtctacaatt tccacgggcg ccattggccg gttctcgacg cgcgaatcga
    13501 gctggagcag ctggtatttt ccggagcgcg cgttctgaaa actttcgagt gacggcgtcg
    13561 cggagccaag cagcacaacc gcgccttcga gtttcgcgcg caccacggcc acgtcgcgcc
    13621 cgtgatagcg tggcgtttct tcctgtttgt agctggcctc ctgctcttca tcgacaacga
    13681 tcaggccaag attttcgact ggcgcaaaca cagcagagcg cgttccaacc acgacgcgcg
    13741 cctcgcctcg ccgcacgcgc caccattctc gcgcgcgctc cacttccgcc agcgcactgt
    13801 gcagcacggc aacgcccgca ccgaatttcg cgcggcacaa tcttcccacc cacagcgtca
    13861 gcgcgatttc cgggaccaga atcagcgctg tcttattgcg cgcaagcact tcttcgacgg
    13921 cgttgagata cacttccgtt tttccgctgc cggtaacgcc gtaaagaagg ccagcggtaa
    13981 aaacgcccgc gccaagccag ccgcggatga ccgcgagttc cctttcctga tccacattta
    14041 gaatattgga cggcggcgtg taatccacat cgaacaaatc ttcttcaatg gcgattggct
    14101 cttcccaaca tttcagtttt tcctggtgga tcattttttc aattagaggc cgcgaaacct
    14161 ttgcgagttt tgcgaggtgc ggaaacggca acggtccgcg ctcttccgcc agcacatgaa
    14221 acacgcgcgc ctctttttcg ggaagcgcgg cgctcggatc ggctgacggc gtcacttcgg
    14281 aagaaacagc cgtattccag gccacaattt tttgcacgcg cgtcttgcgg tgtacggcaa
    14341 cttcgtggct ttcgagctgc ttacggcgca acagccgttc cgccgccgcc tccccaccgg
    14401 gcaacttgtg caggcggtcc gcgcgaaccg ggcggccctc aatttccatc agcgagagca
    14461 gcgccatctc cgccacttcg gcttcgctgc gattgcccag cgaatggagt tgggcaaggt
    14521 gaatgcgccc tgcactcgtc aacaaaaatt cgcgctcg
//
LOCUS       DASZMB010000024         3454 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3319219, whole genome shotgun sequence.
ACCESSION   DASZMB010000024 DASZMB010000000
VERSION     DASZMB010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3319219"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(285..701)
                     /locus_tag="VG272_00955"
     CDS             complement(285..701)
                     /locus_tag="VG272_00955"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HWF12275.1"
                     /translation="MTLPLQITFRNIKHSAEIESWIRAEAEKLETFYHRIIGCRVAIE
                     VPHRHHRKAKPLHVRIDLTLPGKEIVITQEPVALRRTLVKREGLGTTRAKPGPLHGDM
                     EFAIHEAFKSAGRRVKDFARRQRGEVKNHDVFLVEV"
     gene            900..2633
                     /locus_tag="VG272_00960"
     CDS             900..2633
                     /locus_tag="VG272_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HWF12276.1"
                     /translation="MKFNVLALDYDGTIARDGVLDPGVRSAIAEARKQGIIVVIVTGR
                     ILADLERAAGNLHFVDAIVAENGAVLWFANGHSRQLSYSTSTKFLHELSRRGLNYSAG
                     QSIVELDAADAPQTLAIVRELELPLVFLFNRSRMMVLPQSVSKGAGLREALSTLRLSV
                     HNAIGIGDAENDHDLLATCEHGVAVRWGSKLLQEAADEVLEGDGPAAVAGYIRRAAQN
                     LRLPTEKIGQHRLSLGTAEDGHELTLAIRGMNTLIAGDPGSGKSWVAGLMCEQLILQG
                     YSLCIIDPEGDYRSLETLPGVVVFGGEDPPPELPDLARVLRHPDMSVVIDLSHAPYPE
                     KINYMKSLLPMLSSIRTKTGLPHRIVIDEAHYFLHEPNISALVDLSLGSYTFITYRLA
                     DLQPAVRNVIEYILVKHTMEPREIETLTGMLNARGNCKSMIPDLSSLLIDEAILLPGI
                     DELDGQCSKFRLFPRLTPHVRHKMKYLDLQLLVEQGFWFTDRGKLIDRPAKTLSSFLS
                     LLEIVSPDVLAAHAQHGDFSRWIANVFHDHALASDIRKVEQRFRLGHVYDLTTSISKL
                     VRDRYELSPAS"
     gene            2823..>3454
                     /locus_tag="VG272_00965"
     CDS             2823..>3454
                     /locus_tag="VG272_00965"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF12277.1"
                     /translation="MRMRWVVVSLLFCATTINYLDRAILGVILPEIRDKFHFGLPVYG
                     TIQMVFQLAYAGGSLLGGRLLDRYGTRIGYGLAAAEWSLAATLNAFAGSAFQFGLFRT
                     VLGFGESANFPACSKAAAEWFPPEERATAMGIVNAGTNLANIFGPPLFILIAFTWGWQ
                     ACFAIMGGLGFLWLPLWFLSYRLPKQTGPADLLGAKISIREVMKYKQAWG"
BASE COUNT          736 a          904 c          990 g          824 t
ORIGIN      
        1 cgcatcaagc tgcctttgac gcacgaagaa atcgcggagt tcatcggcac gacgcgtgaa
       61 accgtgacac gtacgttgag cgacttcaag gtgcgccatc tggtcgcgat tcaaggatcg
      121 acgctgatga ttcccaatcg cgcggcgctt gaatctttcg cgaacgtttg atttattgga
      181 cgtggcatcg gctgcagcct gtgcggggtt aggccttgct agaactaaaa ccccataggc
      241 cgaagccgat gccacagaac gggacttagc gctttccttc ttcttcagac ttccaccaaa
      301 aatacatcat ggttcttcac ttcaccgcgc tggcggcggg caaaatcctt gacgcgccgc
      361 ccggcggatt tgaatgcttc atggatcgca aactccatgt cgccatgcag cggtccaggt
      421 ttcgctcgag ttgtgcccaa tccttctcgc ttcacaagag tgcggcgaag cgcaaccggc
      481 tcttgagtaa tgacgatctc ttttccgggc agcgtcaaat cgatgcgtac gtggagcggt
      541 tttgccttcc tgtgatgccg gtgagggacc tcgatggcca cgcggcaccc gataatgcgg
      601 tgatagaacg tttccagttt ttcggcttcc gcgcgtatcc aactctcgat ctcggcggag
      661 tgcttgatgt ttcggaacgt gatttgtagt ggaagcgtca tggctatccc cttgttcggt
      721 aatcgagcag ttccaacctg ccgtccccga tctacttacg catgtatgct ggctgagcgt
      781 ttccattttt gatgtgtctg gcatcacagc ccaaagtgac tattctcacg gattagggat
      841 agactaaagt ggaaagtaaa agtgcgacga ctcgcggagc gcccgcctca ggaagcctca
      901 tgaaattcaa cgttctcgcg ctggattacg atggaacgat cgccagggat ggagtgcttg
      961 acccaggggt tcgttccgca atcgcagaag cacgaaagca agggattatc gtggtaatcg
     1021 ttaccgggcg tattcttgcg gatttggagc gcgctgctgg aaacctgcac tttgtggatg
     1081 cgatcgtcgc ggaaaacggc gcagtgctat ggttcgcaaa cggacattcg cggcagcttt
     1141 cgtactcgac gtccacaaaa tttcttcatg aactcagccg gcgcggcctg aattacagcg
     1201 ccgggcaaag catcgtggaa ttggacgccg ctgatgcgcc gcaaacgctt gccatcgttc
     1261 gcgaattgga attgccactc gtttttctgt ttaaccgcag ccggatgatg gtcctgccgc
     1321 agtccgtgag caaaggcgcg ggcctgaggg aagctctctc gacgcttcgg ctttccgttc
     1381 acaatgccat cggaatcgga gacgcggaaa acgatcatga tttgctggcg acgtgcgagc
     1441 acggcgttgc cgtgcgctgg ggcagcaagc tattacagga agccgcggac gaggtactgg
     1501 aaggcgacgg ccctgctgcg gtggccggat atattcggcg tgccgcacag aatctgcggc
     1561 tgcccacgga aaaaattggc cagcaccgct tgtcgctggg aactgcggag gacggccacg
     1621 aactgacgct ggcgattcgc ggcatgaaca cattaattgc gggggacccg gggtcgggga
     1681 aatcctgggt cgctgggctc atgtgcgagc agttaattct gcagggatat tctttgtgca
     1741 ttatcgatcc ggagggagac taccggtcgc tcgagacgct gccgggtgtc gtggtttttg
     1801 gcggcgagga tcctccgccg gaactaccgg acctggcacg cgtgctgcgt catccagaca
     1861 tgagcgttgt gattgacttg tcgcacgcac cgtacccgga aaaaataaat tacatgaaat
     1921 ccctgctacc aatgctgtct tccattcgca caaaaaccgg gctgccgcac cgcattgtga
     1981 tcgacgaggc gcattatttt ttgcatgaac caaatattag cgcgctggtg gatttgtcgc
     2041 tgggatccta tacgttcatt acgtaccgcc tggcggacct gcaacccgcc gtgcgaaacg
     2101 tgattgaata catcctcgtg aagcatacga tggagcctcg agaaattgaa acgctgacag
     2161 gaatgctgaa tgctcgggga aattgcaagt cgatgattcc cgatctctcg tcactgttga
     2221 ttgacgaagc aattcttctg ccgggaattg atgagctgga cggccagtgt tcaaaattca
     2281 ggctgtttcc gcgcctgacg ccgcacgtac ggcacaaaat gaaatacctg gatttgcaat
     2341 tacttgtgga gcaaggcttc tggtttacgg acagaggaaa gttgattgac cgccccgcga
     2401 aaaccctttc gagtttttta agtctcctcg aaatcgtatc cccggacgtc ctggcggcgc
     2461 atgcgcagca cggggatttt tcgcgatgga tcgcgaatgt attccatgat catgctctgg
     2521 catcggatat tcggaaagta gagcagcgtt tccgcctcgg ccacgtctat gatctcacca
     2581 cgtcaatttc caaattggtg cgagataggt acgagctgtc gcccgcaagc tgactgcatt
     2641 tatagatcga gactccgcat aactcactac acacggctta ccaaagaacg gcgatcttcc
     2701 ggttggcgcg tcgcaaaacg tcaatcggga gattggcgtt ccggggaaac ataaccttct
     2761 tggcgtaaca atcactcaaa tgatttatgt aatcgaaaca catgcagtat gatggccacc
     2821 aaatgcgtat gcgttgggtg gttgtaagcc tcctgttttg cgccactacg attaattatc
     2881 tggaccgcgc gattctaggc gtgatcctgc cggagatccg cgacaagttt cattttggct
     2941 tgcctgtgta cggcaccatt cagatggtat ttcaacttgc atacgctggc ggatcgctgc
     3001 ttggcgggcg cttgctggat cgctacggga cgcgaatcgg ctatggcctc gcggcggccg
     3061 agtggtcgct ggccgcgacg ctcaacgcat ttgcaggcag cgcgtttcaa tttggattgt
     3121 ttcgcactgt tctaggcttt ggcgagtccg cgaattttcc ggcgtgcagt aaggcagctg
     3181 cggaatggtt tccgcctgag gaacgcgcca ccgccatggg aatcgtgaac gccggcacga
     3241 atctggcaaa tatttttggg ccgccgcttt tcattctgat cgcgtttact tggggatggc
     3301 aggcttgttt cgcgatcatg ggcgggctcg gattcctgtg gctgccgttg tggtttctgt
     3361 cctatcgatt gccgaaacaa acgggccctg cggatttgct gggcgcaaaa atttcgatcc
     3421 gcgaggtgat gaaatataaa caagcgtggg gcta
//
LOCUS       DASZMB010000025         8226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2777816, whole genome shotgun sequence.
ACCESSION   DASZMB010000025 DASZMB010000000
VERSION     DASZMB010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8226
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2777816"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="VG272_00970"
     CDS             complement(<1..1088)
                     /locus_tag="VG272_00970"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HWF12278.1"
                     /translation="MATAVLETRLNLLWETPKTVRGWFATVDHKELGIRYLVTAFAFL
                     IIGGIEALLMRIQLSRSEMSILSPEAYNQIFTMHGATMIFWYASPILSGFSVYLVPLM
                     IGARDMAYPRLNAFTYWAYLFSGIFLYIAPVLGQAPHAGWFAYVPYTLTTYSPGIGMD
                     VYALALIFLTISTTGGAINFIVTILRLRAPGMAISRMPLFLYSTLTVSVVILFALPAL
                     TAACVFLELDRRWGTHFFNVARGGETFLWQQLFWFFGHPWVYVIFLPATGMISLIVPV
                     FSRRPIVGYPFVAISTVLTGVVGFGVWLHHMFTIGMSDMAMSFFSAGSMTIALFTTVQ
                     VFAWVATLWTGRPVPTASMHFALGSIALL"
     gene            complement(2090..2380)
                     /locus_tag="VG272_00975"
     CDS             complement(2090..2380)
                     /locus_tag="VG272_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12279.1"
                     /translation="MQYHLPTPFDSVENTQEYFGLLSETLEEVKQEVDRDITAASEAR
                     QQRRLEALRLVHFKLQKLEDHVKASSLLLNDLRTLRRLLLQERVRTEEMKMR"
     gene            complement(2478..3275)
                     /locus_tag="VG272_00980"
     CDS             complement(2478..3275)
                     /locus_tag="VG272_00980"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWF12280.1"
                     /translation="MEPTVQADRGSAERTVPADGHGLTAGEIKTPRRFLNQGIAAQLM
                     SPLSLVRRFLIRMYSLWVCKTYPFASVGSDVTIHFRSELYRSQAHRIALGNSVFLDKD
                     ANLRAHAPSEEDVDPILTIGDGSVIGPRCFLSAKNCIQVDSEVVLEPSVLIQDHSHAY
                     KDVNVTIRRQGVTEGGRIKISQGCWIGQGTVIHCDQGELTLGPNCVVAPNSLVNRSFP
                     GYSLVAGNPARVVKQYDMEKGVWVLGSSRPAAESESSQRRPELQSIR"
     gene            complement(3718..8214)
                     /locus_tag="VG272_00985"
     CDS             complement(3718..8214)
                     /locus_tag="VG272_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWF12281.1"
                     /translation="MLKNELEKPLREVMWGAATEVLEQTAYTQPALFALEYAMSEMWK
                     SWGIVPGVVLGHSVGEYVAACVAGVYSLADGMKLIAARARLMQRVSGRGAMAAVMAKE
                     ERVREALKGLEARVSIAGLNAPESVVISGYEEEMGIAEAKLKDAGVRVQRLAVSHGFH
                     SPQMAEMEAEFEAIANEIKYAAPRVKLISSVTGREVGREEIRGSYWRGQVREPVRYQK
                     AMETLRESGQRVFVEVGPGSTLIGLGKQSVEGEEETGKPERLWAVSIRKSKQEWEQVL
                     ESLGKMYERGAEVDWEGFDRGYGRRRVELPTYPFQRQRYWIENPVQRNKRISSRADTR
                     NVPDDWYYQLEWEPKPHVPAKHNFDEAQRAARWLIISENKAAAKKLRAHLEAVGGTAS
                     VAHSREATLEQLLNGSYDSVVQLCTQQDSDSKTLNPDLQSTGQPSLISTVLATAQAIL
                     SQNSKAKLWLITSGAQPVTKTQTILNIAQAQVWGFGRTFALEHPDRWGGLIDLDPSDE
                     RGENLSEIISWILRPDAEDQLAFRGGKRHGLRLTRSVPLSNSEKPHFLPNKNYVITGG
                     LGSLGLHAAEWMVERGARNLILIGRRTPSAEAQIKIETLKGRGARIEVRTADVASKWS
                     VNAAFHEIIRTMGSVDGIVHAAGYIDDGLLEQQTWERMEKVLAPKVAGAWNLHEATTG
                     MKLDFFILFSAFAALTGSAGTSGYAAGNAFMDALAHYRKSLGLPALSINWGAWEGDGM
                     GKHVGGAKSRRTGISFMPPRLALAALGSTLSISDPQIAIGALDWKSLKPTFEGSAKRP
                     LLEHLSAEISGIGKLPASAQVPAKSHLQELSSTSPEDRKKNLVQYLIAAVAPILGVEP
                     AAIDPSRPLTDFGVDSLMALEFRNRLNSDLGVTIPTVRFLEGITLQEVAAQIVKESPA
                     ASVPNSSSSDSTSSSQQCASSAIRPLDEVLRVASEKRQSWLEDYLVPALAAILGVEPA
                     SVTSMRSISEYGLDSLMALELKNKIAADLSVTVPTVRFLEGPSLREVAAQIANELPNA
                     SVISAPPEVSEASIEYPLSFAQQQAYFGHKLMPDSAAFNVGFTAKASPHLDWPVFQRS
                     VVKLLKRHPALRAVIVESDRGPVQRILPSDTPVASLIDASSIQESELKEQITRDFQKN
                     FKLDQPLFRVNVFRLANCDIMSFKVDHIIIDHWSVQLCVEDLKKIYSAELAGTEAVLP
                     AVKGEYQNFVAWEAEMSQNAELWGYWKEKLGGVLPQLDIPSPRHRPAALLARGEAIPL
                     LLPPELSAQIKKVARPLKATSYTFMLAAFQLLLHQFSQQDDIIVGTSVTGREDARWTN
                     TIGFFVNVLPLRANFTSQQTFADHLMRSRETVLGALQHQEFPFAAMITRLGLGRSLDR
                     IPVFQAFFNFLTDHAGEFGRLFMGAEGAPVPFGGSELFPWLTLPLQEGRVEVGVQLVE
                     VQGHVDGYLNYNSDVMDRATAEAMAVAYGKILEAVVRDPNMPIETLVPGSEELVTERE
                     EFAL"
BASE COUNT         1844 a         2358 c         2136 g         1888 t
ORIGIN      
        1 agcaacgcaa tcgaccccag cgcgaaatgc atcgaggcgg tgggaacggg gcgaccggtc
       61 cacagcgttg caacccaagc aaatacctgc acggtggtaa acagcgcaat ggtcatgctg
      121 ccggcgctga aaaaactcat cgccatatcg gacattccaa tcgtgaacat gtggtgcagc
      181 catacgccga atccaaccac gccggtaagc accgtggaaa tcgccacgaa tggataaccg
      241 acaatcgggc ggcgcgagaa gacgggaaca atgagcgaaa tcattccagt ggcgggaaga
      301 aaaattacgt acacccacgg gtgcccaaaa aaccagaaaa gttgttgcca caggaatgtc
      361 tcgccgccac gcgcaacatt gaagaagtga gttccccatc gacggtccaa ttcgagaaag
      421 acgcacgccg ccgtcagcgc cggcagcgca aataaaataa ctacagaaac agtcagcgtg
      481 ctgtaaagaa acagaggcat ccggctgatg gccattccgg gtgctcgcag cctcaggatc
      541 gtcacaataa aattaatggc tccgccggtg gtggaaattg tcaaaaaaat cagcgctaac
      601 gcataaacgt ccatcccaat tcccggcgaa taagtcgtta gtgtgtacgg aacgtaagca
      661 aaccaccctg cgtgcggtgc ctggcccaaa actggcgcga tatagagaaa aatgccagaa
      721 aataaatatg cccaatatgt gaatgcgttc aaccttggat aagccatatc acgcgcgcca
      781 atcatgaggg gaaccaggta aacggagaag cctgacaaga tgggagacgc ataccagaaa
      841 atcattgttg cgccgtgcat cgtaaaaatc tgattgtagg cttccgggga aagaatcgac
      901 atctctgacc gcgaaagctg aatgcgcatt aaaagcgctt ctattccgcc gattatcaaa
      961 aacgcaaagg ctgtgacaag gtagcgtatt cctaattcct tgtgatccac cgtcgcaaac
     1021 cagccgcgaa ctgttttagg agtttcccac agtaaattta gccgggtctc aaggacagct
     1081 gtcgccatgg cagggtgatc cgcgaaattc tatctagcag gagtgaggtg ctctccccat
     1141 ttgcatggtg actcctgctt ccctgtcgca caatacattc aatccactag aatgatcgcc
     1201 aattttggag tatggaacct accgtttacg gctaccctcg tgcgttaaca cgtatccctt
     1261 agaaagacat attcatatat ttttaaatcg ctccgaaaag cgcgggtggt tgcagtattg
     1321 actggccctg tccttggatt cgcggttagg atttcgagcg cggtaactac gttgaggggg
     1381 tgtgtatctg gcgtcacgat catggcatct gccaaacaca aacttgcaga tcgagacttt
     1441 ggtgcgcgcg tatccacgcc gaactatgcg agatgtgtaa ggatgcccaa gtgtacgtta
     1501 tgcgctgggc gtgtctactt tttcgtttaa aaagttctgt gcattatctt gtgagcgggc
     1561 gctcggctaa tttgaactta cttcctcatt ttttgagggc ctactttgat taatgcttga
     1621 tcgcagttaa acgattgctg ggtgttgtag gaactgactc ctgggccgcc ccggcctttt
     1681 ttccgcattc agagtgataa cattgaccct tgaaatgtac cgcatccggg tgggacaggt
     1741 tcagtggctt gttgcacttc gggcagatgc ccatctgtac gctagttttg ttcgttgtac
     1801 tctcccttgc tgtagacgcg ctcgttttct atccgtcgat tctcgcgacc ggcgcttcgt
     1861 tcgtggctaa gcatcctgtc ctccacgtaa tggtgttata tcacgttggt tggcaaacaa
     1921 tcggataatc ggggtccgtg ccctcccacg cggttgaaac atggctcgga aatggagcct
     1981 cgcaagccaa tcatcaggtt cttagggcgt tctttaatta cattggccaa ggaaattaca
     2041 ttggccaagg aacgaaccgg agtggaccta gcagcggtga gatggaaatt tatctcattt
     2101 tcatttcttc ggttcgtact ctttcctgca ggagaagcct tcgcagcgtg cggagatcgt
     2161 tgagcaagag actgcttgct ttgacatggt cttccaattt ttgcagcttg aaatgcacga
     2221 ggcgcaaggc ctccagcctg cgctgctgcc gtgcttcaga ggccgctgtg atatcgcggt
     2281 cgacctcctg tttcacctct tcgagagttt cggagagcaa tccgaaatat tcctgggtgt
     2341 tttcgacgct gtcgaatgga gtggggagat gatattgcaa aggatgtacc ttccatttta
     2401 aattatcgtc tggctttgcg gccggctcgg ctcagcgaca gcgtcctttg gcatcgaccg
     2461 tttgcggcaa ggcgcgctca ccgaatggat tgcaactcgg gtcgccgttg tgacgattcg
     2521 gactctgcag cagggcgcga cgagccgaga acccagacgc ctttttccat gtcatattgc
     2581 ttgacgactc gggccggatt gcccgcgacg agtgagtaac ccggaaagct gcgattgacg
     2641 agcgaattgg gcgcgacgac gcagtttggc ccgagcgtga gttcgccttg gtcacagtgg
     2701 atcacggttc cctgcccgat ccagcagccc tggcttatct tgattcgacc accttcggtt
     2761 actccctggc ggcgaatggt cacgttcacg tctttatagg catgactatg atcttgaatc
     2821 agcacggaag gttcgagcac aacctcgctg tcaacctgta tgcaattctt ggcagacaaa
     2881 aaacaacggg gaccgatgac ggagccgtcg ccgatcgtca aaatcggatc gacgtcttcc
     2941 tccgatgggg cgtgcgcacg aaggttcgcg tccttatcca aaaagaccga attgccaagc
     3001 gcgatgcggt gcgcctggct tctatacaac tcgctccgga agtgaatcgt tacgtccgag
     3061 cctaccgatg cgaatggata tgtcttgcaa acccataaac tatacattct gatcagaaat
     3121 cgacgaacaa gcgacagtgg ggacatgagc tgagccgcga ttccttgatt aagaaatctg
     3181 cgtggcgtct ttatctcccc agccgtcaac ccatggccat cggcaggcac cgtgcgctcc
     3241 gcactgccac gatcggcttg aactgtcggt tccataccct gcgatgagca atcggtagac
     3301 catgcgggtg ccgacgaccg tgtggcagcc gtcacaccgc aaagcgtaac aagctgatta
     3361 caatccgttt agacagaggg ccaaatgggg agtggcgcgc gagaactctt agacttagat
     3421 gtcggactcg acctgaggaa agaatttacc gtaataagga aaacggatta agtccaaccg
     3481 gctcgcgaaa atcacatggg aatcacgacg gtgagcgccc aattgccacg caggccgtcg
     3541 aggctggaaa tttcttagcg gggtaatttg ccagcaacgg gtgagacgca tttctcctta
     3601 tgcgcatcca ctcgcggcaa ttcgtctatg acgccggcta ccgtccaggt gttcggctgc
     3661 gtcgcgcaac cgaacctcca acaaacttcc agacgcgctc tcacgggaat tgttaaatta
     3721 caaggcgaat tcttcgcgtt cagttacaag ttcttctgaa cccggcacca gggtttcgat
     3781 tggcatattg ggatcgcgca cgacggcctc gagaattttt ccataggcca cggccatggc
     3841 ttccgctgtc gcgcgatcca tcacatcgct gttgtagttc agatagccgt ccacgtggcc
     3901 ctgcacttcg accagttgca ccccaacctc aacccggccc tcctgcaacg gcaacgtaag
     3961 ccatggaaaa agttccgagc cgccaaacgg aaccggcgcg ccttccgcgc ccatgaagag
     4021 gcggccgaac tcgcctgcgt ggtccgtgag gaaattgaag aaggcctgaa acacgggaat
     4081 gcgatccagg ctccgcccaa gcccgaggcg ggtaatcatc gcggcaaaag gaaattcctg
     4141 atgctgaagc gcgccgagca ctgtttcacg gctacgcatc agatggtctg caaatgtttg
     4201 ctggctcgta aaattggcgc gcaacggcaa aacgtttacg aagaatccga tcgtgttggt
     4261 ccaacgagca tcttcgcggc ccgtcacgga ggttccgacg atgatgtcgt cctgctgact
     4321 aaactggtga agcagcaatt gaaacgcggc cagcatgaat gtgtacgaag tcgcttttaa
     4381 tggccgcgcc actttcttga tctgtgcgga cagctctggc ggaagcagaa gtgggatggc
     4441 ctcgccgcga gccagcagcg ccgcaggtct gtgacgcggt gaaggtatat ccaactgcgg
     4501 caggacgcca ccgagttttt ctttccagta accccacaat tcggcgttct ggctcatctc
     4561 cgcttcccac gccacgaaat tctgatactc gcccttcacc gcaggaagca ccgcttctgt
     4621 gcctgcaagt tccgcggaat aaatcttttt caagtcttcc acgcacagtt gaacggacca
     4681 gtggtcgatg atgatgtgat ccactttgaa ggacatgatg tcgcaattcg cgaggcggaa
     4741 tacgttcacg cggaacagag gttgatcaag tttaaaattt ttctgaaagt cacgagtgat
     4801 ctgctccttc aactcgcttt cctgtatgga acttgcatcg atcaatgacg cgactggcgt
     4861 atcggacggc aaaattctct gcacgggtcc gcggtccgat tcaacgatta ctgctcgtag
     4921 tgcggggtga cgcttgagaa gtttcacaac cgaccgctgg aaaacgggcc agtccaggtg
     4981 cggcgacgcc ttcgcggtaa atccaacatt gaatgcggcg gagtctggca tgagcttgtg
     5041 gccaaaataa gcctgctgct gagcaaaaga taatgggtac tcgatgcttg cttctgacac
     5101 ttctggaggg gcggagataa ccgaagcgtt gggtaattcg ttggcgattt gcgcagcaac
     5161 ctcgcgcaga ctggggcctt caaggaaacg cacggtggga accgtgacac tcaggtcagc
     5221 ggcgatttta tttttgagtt ctagcgccat cagcgaatcc aagccgtact cactgatcga
     5281 gcgcatcgaa gttacgcttg caggctcaac cccaaggatt gctgcgagtg ccggaacgag
     5341 atagtcctct aaccacgatt gacgtttttc ggaagcgact cgaagaactt cgtccaatgg
     5401 cctgatcgcg gaggaagcac actgttgcga cgaggaggtt gaatccgacg aactggaatt
     5461 cggaactgac gcagccggcg attcttttac gatttgagcg gcgacttcct gaagcgtaat
     5521 gccttccaga aaacgcaccg tgggaatcgt gacgccaaga tcggaattga ggcgattcct
     5581 gaattccagc gccatcagcg agtctacgcc gaagtccgtc aagggacggg agggatcgat
     5641 ggcggcaggc tcaactccca ggatcggcgc gacggcggca atcaaatact gaactaaatt
     5701 tttctttcgg tcttcgggcg acgtgctgct caattcctgc aagtggcttt tcgcaggcac
     5761 ctgcgcagaa gcggggagtt taccaatacc agatatttct gcggaaaggt gttcaagcaa
     5821 aggccttttc gccgagcctt caaatgtggg cttcagagat ttccagtcca acgcgccaat
     5881 cgcaatctgg ggatcagaaa tggaaagcgt ggagcccagt gctgcgagcg ccaagcgtgg
     5941 aggcatgaaa ctgatcccgg tgcgccggct ttttgcgcca ccgacgtgct tgcccatccc
     6001 gtcaccctcc cacgctcccc aattgatgct gagagcggga agtccgagcg atttacgata
     6061 atgcgcgagc gcgtccatga aagcgttgcc cgccgcgtag cctgacgttc ccgcggagcc
     6121 cgttaacgcc gcgaatgcgg aaaataaaat gaaaaaatcc agcttcatgc ccgtggttgc
     6181 ttcgtgcaga ttccatgcgc cggcaacctt gggcgcgagc actttttcca tgcgctccca
     6241 agtttgctgt tccagcaggc cgtcgtcgat ataacctgcg gcatgcacga ttccgtcaac
     6301 tgatcccatg gtgcggatga tttcatggaa cgccgcgtta acggaccatt tggacgcgac
     6361 gtcagcggtt cgcacttcga tacgcgcgcc acggcctttc aatgtttcga tttttatttg
     6421 cgcttccgcg gaaggcgtgc ggcgcccgat caatataagg tttcgcgctc cgcgttccac
     6481 catccattcc gccgcatgca gacccagact gcccaggcca ccggtaatta cataattttt
     6541 attgggaaga aaatgcggct tttcggagtt ggacagcggc acactgcgcg tgagccgcaa
     6601 tccatggcgt tttccgccgc gaaatgccaa ctggtcttct gcgtcgggcc gcaaaatcca
     6661 agaaattatt tcggaaagat tttcgccccg ttcatcgctg ggatccaggt cgatgaggcc
     6721 gccccaacgg tccggatgct cgagcgcaaa cgtccggcca aaaccccaaa cctgcgcttg
     6781 ggcgatattc agaattgttt gcgtcttcgt gactggttgc gcgccggacg taatcagcca
     6841 aagttttgct ttactgtttt gcgagaggat cgcctgcgca gtcgccaaca cggtcgatat
     6901 taatgagggc tgcccggttg attgcaagtc aggattcaga gttttactgt cggagtcttg
     6961 ctgcgtgcaa agctgcacaa cggaatcata ggagccatta agtagctgct cgagtgtggc
     7021 ttcgcgcgaa tgcgctacgc tcgcggttcc tccgactgcc tccaaatgcg cgcgtaattt
     7081 tttggcggca gctttatttt ccgaaatgat aagccagcgc gccgcgcgtt gtgcttcatc
     7141 gaaattatgt ttcgccggaa cgtgtggttt cggttcccac tccagttgat agtaccaatc
     7201 gtccggaaca ttccgtgtgt ccgcgcgcga ggatattctc ttattgcgct gaactggatt
     7261 ttcaatccag tagcgctggc gttggaacgg atacgtgggc agctccacgc ggcgccttcc
     7321 gtatccccga tcgaatcctt cccaatccac ttccgctccc cgctcataca tttttcctaa
     7381 actttccagt acctgttccc attcctgttt acttttgcgg atcgacaccg cccacaatcg
     7441 ctccggcttc cctgtttcct cttccccctc cacgctctgc tttcccagtc cgatcaacgt
     7501 acttcccggc cccacttcca caaatacacg ctgccccgat tctctcagcg tctccatcgc
     7561 cttctggtac cgcaccggtt cccgcacctg cccccgccag tagcttcccc tgatctcctc
     7621 gcgccctact tccctccccg tcacgctcga tatcagcttc actcgcggcg ctgcatactt
     7681 aatttcattc gcgatcgcct caaactccgc ttccatctcc gccatctgcg gcgaatggaa
     7741 gccgtgcgac accgcgagcc tctgcacccg cactcccgca tctttcagtt tcgcttccgc
     7801 aattcccatt tcctcttcgt accccgatat caccacgctt tccggtgcat tcaatccggc
     7861 aatcgatacc cgcgcttcca atcctttcag cgcttcccgc acgcgctcct ctttggccat
     7921 caccgccgcc atcgctccgc gcccgcttac cctctgcatc agtcgcgctc gcgccgcgat
     7981 cagcttcatt ccatccgcca ggctgtacac gcccgccacg cacgccgcca cgtattcgcc
     8041 cacgctgtgc cccagcacca cgcccggcac tatgccccaa ctcttccaca tctcgctcat
     8101 cgcgtattcc agcgcaaaca gcgctggctg cgtgtacgcc gtttgctcca acacttcggt
     8161 cgccgctccc cacatcactt cgcgcaacgg cttttccagt tcgttcttca gcaattcctc
     8221 gcactc
//
LOCUS       DASZMB010000026         9162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1118270, whole genome shotgun sequence.
ACCESSION   DASZMB010000026 DASZMB010000000
VERSION     DASZMB010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 9162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9162
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1118270"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..561)
                     /locus_tag="VG272_00990"
     CDS             complement(64..561)
                     /locus_tag="VG272_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12282.1"
                     /translation="MTRRGSLVYYLASWICGGLFLTIAMFLQERASPQEMGMGPTSAG
                     AAIITTYFLVLVFCAFLSVVFAFFLRLIMRVLHAESMWQWMLAGTVLAMPMVFAVRWA
                     SRMMDSSRVQGVGREIAVLLFLGVRGIAENHPLLGLPVAALNAGVLFLIHRAFGREDA
                     LLDKK"
     gene            complement(623..2221)
                     /locus_tag="VG272_00995"
     CDS             complement(623..2221)
                     /locus_tag="VG272_00995"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HWF12283.1"
                     /translation="MSAQPMSSPQPDRVQFEAAAPRITLRNHFAHPYDSAIAAARTCY
                     APRLINPEEVTDKQRLQIGASTFYGGHHTVFQHAHFEFGLENVSRQFVWSFLHAHPFY
                     NSEQQSQRYVKLDHAQAYLPAETPGFGAGERAIYEDAIARAWGYYRELTALLRGDAHE
                     ILSDIWHIGPMSHAKRVLKVDRQAEKRAIEVARYVLPVAAATTMVHTLSGIVLHRLWR
                     MAAASDTPTEARAVIGEMVSRVREIDPQFFDRFDNVPLEQEELPEWSHAKSAGSAAHA
                     EEFAREFDARLAGRVSLLTDYSPHAPRVLADAYRAVVGLTESACPDDEAIDRLLNPAR
                     NPYRLETLNIGVHAPIMRALQHANFTFAKKISHTADSQDQRHRMVPGSRPLLMLSDTR
                     SPDFVVPMLIAGNARAREVYERAMADAWAAKNELLDRGVPREIALYLLPNAKAIRLIE
                     TGSLLHLLHKWTMRTCFNAQEEIYQASMEEVEQIRAVLPSLGRYLGPPCYIRAGVATP
                     ICTEGSHFCGVKVWEEFPKIERRI"
     gene            complement(2292..2543)
                     /gene="rpmE"
                     /locus_tag="VG272_01000"
     CDS             complement(2292..2543)
                     /gene="rpmE"
                     /locus_tag="VG272_01000"
                     /inference="COORDINATES: protein motif:HMM:NF000612.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HWF12284.1"
                     /translation="MKPNIHPDYHPVTVHCACGHTFPTRSTMKGEFLRVEICSNCHPF
                     FTGKQKLLDTAGRVERFTKKYEKAAEAQQAIKDATKKKR"
     gene            2664..3170
                     /locus_tag="VG272_01005"
     CDS             2664..3170
                     /locus_tag="VG272_01005"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWF12285.1"
                     /translation="MIYMWVGNSFGCPLGRKRFTAQAKHYSPGGEKMTMHSIRKFTQR
                     PLLLVCAALLLASCSFAGWALAQEPAAHDRIYVVTHVDIVPPNTAAGTKLVQQYVADS
                     RKDKGFVRIEAGAQISRVNHISIVEVWQNEKAFDEHVAAAHTKEFRRQIDPMLGSPYD
                     ERLHLSLE"
     gene            complement(3250..4380)
                     /locus_tag="VG272_01010"
     CDS             complement(3250..4380)
                     /locus_tag="VG272_01010"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWF12286.1"
                     /translation="MDTLATMDPSGRGQFLQRFFRSIAQIELTETQSLDYWEQTLARK
                     NELSQSLGKRVTLQAALAEVLASNGQLRMPILMEYEELRKLQFNATTDPLTSLYNRRF
                     FDDYFEKELNRSSRYNHKLALVMLDLHRFKDVNDKFGHQQGDALLQMAAATLRKSLRT
                     SDYAFRIGGDEFALLLPQSDTEQAATLSRRLRSTYASSLEPMNLGIPLALDYGLAVYP
                     DDGELQEALIRVADARLYQLKNESKAAAGAGSRTGPTERNQRITPAGPLQNLPYVEPS
                     TESETKKTTDRRRWERFSMANTNAYALIGENPERKARVLDLGHGGICLESLNPDEFAE
                     AFHAVLHVPILPPVRVNLRRVYRAIQPQADSGATRIGCAFIT"
     gene            complement(4709..5029)
                     /locus_tag="VG272_01015"
     CDS             complement(4709..5029)
                     /locus_tag="VG272_01015"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWF12287.1"
                     /translation="MSTSTFERRRSQRIHVQIPMFVRGTDAAGLEFLDLIKTLDISAT
                     GAHLASSRAVRMNELVNLTIPAPSSPSATLPPDTPPIQARVRRHRAADGIHLLGVEFV
                     KSLG"
     gene            5692..6627
                     /locus_tag="VG272_01020"
     CDS             5692..6627
                     /locus_tag="VG272_01020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWF12288.1"
                     /translation="MARIQWLPEADWLVVRIEEAEKRPLQFTQNDATATKHTVQELLQ
                     EREITYRNLLAAYLRLQEVNRQKTVFLASAAHELKTPLAVIKGYYDLLLSGSLGKLTD
                     KQRDILQESKESCERLVRLVSMFLNYSALESGKLVLHLRENDLRDCLSELATRWHEAF
                     VRKHVRLETILNADIPIFKFDYQKVQQTVANLLDNSLKHTPAGGNVIFRAELYFWERR
                     TAEEIQTLDRRRERTQYPNCIKVSVSDSGPGIASEHHQEIFEDFVRVDRTSSGMGLGL
                     AIAKRLVQAHRGKIWVESEPRRGCTFTFLLPIDNT"
     gene            6658..8142
                     /locus_tag="VG272_01025"
     CDS             6658..8142
                     /locus_tag="VG272_01025"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWF12289.1"
                     /translation="MAEKERILVVDDEPSIRKYLGTLLEVDGYQVEAVNSGLEAIQKI
                     ESGDRPDFIILDVLMPELNGIETLKQLMQIDRSLNVVMLSCSNEVGTVVEAIRLGAQD
                     YLTKPFEKAELDVAMLKCRQKQQLRQENKALREYCDTITEDLCFLAASPQMVRIRQQI
                     LQIAPVDVPVFIYGESGVGKEVVARLIHMRSNRRQQAFIKVNCAALPGELLESELFGF
                     EQGAFTGAVRSKPGKFELATKGTIFLDEIAEMSPHLQAKLLHVLQDHQFSRLGGRQLI
                     DVDVRVLAATNVEIQEAMKSGRLREDLYYRLNVLSINVPPLRERASEIPLLFRHFLQK
                     YSEKFSKEPMVPSQHLMEAAVRYPWPGNLRELENFVKRYVILEDDEGSFRELLEMSAT
                     RQRISPREEAAPVREQGLKALVRSLKDEAEMEAIGDALEKTHWCRKDAAKMLGISYKA
                     LLYKIRQFGLDTGRPARASTKKGAAGSTTPGPAPSNLAPAGRHS"
     gene            8548..8886
                     /locus_tag="VG272_01030"
     CDS             8548..8886
                     /locus_tag="VG272_01030"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWF12290.1"
                     /translation="MTERRTARRYDLSLPVIIRVPVEKEIASRSGKTRDISTRGVYFT
                     IDQDLGTGAELDITLTLPSEVTRGSEVFIRAMGKVVRIDKKPDSETSRVGVAAVIERY
                     EIIRTEPSTH"
BASE COUNT         2200 a         2435 c         2455 g         2072 t
ORIGIN      
        1 gatcagaggc gggacctttc cgccgcgcac ccggatccac attaaagatg caaattctca
       61 gccctatttt ttatctaaca gcgcgtcttc ccgcccaaac gcccgatgaa tcaaaaacaa
      121 aactcccgca ttcaacgcag ccacaggcaa tccaagtaac ggatgattct ccgcaattcc
      181 gcgaacgccg agaaaaagca aaaccgcaat ctctcgcccc acgccctgaa ctctcgaaga
      241 gtccatcatc cgcgacgccc aacgaacggc aaaaaccatc ggcattgcaa ggactgtccc
      301 agcaagcatc cattgccaca tgctttctgc atgcagcacg cgcatgatga gccgcagaaa
      361 aaatgcaaat acaacagaaa gaaatgcgca gaaaacaagc accagaaaat aagtggtaat
      421 aatcgccgcg cccgcgctcg tagggcccat ccccatttcc tgcggcgaag cccgttcctg
      481 caagaacatc gctatggtga gaaacagtcc cccgcagatc cacgaggcga ggtaatagac
      541 gaggcttccg cggcgcgtca ttattttttc agtcggaaat ttctcaaacg atgtggcata
      601 cagggagtca gacgcacaaa aatcaaattc gccgctcgat ttttggaaat tcttcccaca
      661 ctttcacccc acaaaagtgc gacccttctg tgcagatcgg cgtggccacc ccggcgcgaa
      721 tgtaacaagg cggaccgagg tagcgtccca gcgacgggag cactgcgcgg atctgttcca
      781 cttcttccat ggacgcctga taaatttctt cctgcgcgtt gaagcatgtg cgcatcgtcc
      841 acttatgcag caagtgcagc agcgagccgg tctcgatgag gcgaattgct ttcgcattcg
      901 gcagcaggta cagggcgatt tcgcgcggca cgccgcggtc gagcaattcg tttttcgcgg
      961 cccaggcatc ggccattgcg cgttcgtaaa cttcgcgtgc gcgcgcattt cccgcgatga
     1021 gcatcggaac aacgaaatcc ggcgagcgcg tgtccgacag catcagcaat ggtcgcgaac
     1081 cgggaaccat gcgatgccgc tggtcctggc tgtcagccgt gtggctgatt tttttcgcaa
     1141 aggtgaaatt cgcatgttgc aacgcgcgca tgatcggcgc gtgcactcca atgttcagcg
     1201 tctccaggcg atacgggttg cgtgccggat tgagcaacct gtcgattgct tcgtcatccg
     1261 ggcacgcgct ttccgtaagg cccacaacgg cccgataggc gtcagctagc acacgaggag
     1321 catgcggaga ataatcggta agtagagaga cgcggccagc gagccgcgcg tcaaactcgc
     1381 gcgcgaattc ttccgcatgc gctgcagaac cagcggattt cgcgtgcgac cactccggca
     1441 attcttcctg ctccaaaggc acgttgtcga agcggtcgaa aaattgcgga tcgatttcgc
     1501 gcacacgaga gaccatctcg ccgatcacag cgcgcgcttc ggtgggcgta tctgacgcgg
     1561 cagccattcg ccacaatctg tgcagcacaa tgccggaaag cgtatgcacc atcgtcgtgg
     1621 ccgcagcaac gggcagcacg tagcgcgcta cttcgatggc gcgcttctcg gcttgccgat
     1681 ctactttcaa cacgcgcttg gcatgcgaca tcggcccgat gtgccaaata tcgctgagaa
     1741 tttcatgagc atccccgcgc aacaacgccg tcaattcgcg gtaatagccc caggcgcggg
     1801 caatcgcatc ttcgtaaatt gcgcgttccc ccgcgccaaa gcccggcgtc tccgcaggca
     1861 aataggcttg cgcgtgatcc agctttacgt aacgctggct ttgctgttcg gagttgtaaa
     1921 acggatgcgc gtgcaggaag gaccagacga attggcggct tacgttttcc aggccaaatt
     1981 caaaatgcgc gtgctgaaaa acggtgtgat gcccgccgta aaaagtagac gcaccgattt
     2041 gcaatcgctg cttgtcggtg acctcttccg gattgatcag acgcggtgca taacacgtac
     2101 gcgccgcggc aatcgccgaa tcgtagggat gggcgaagtg attacgcagc gtaatgcgcg
     2161 gcgcggcggc ttcaaactgc acgcggtccg gctgcggtga actcatgggt tgagcggaca
     2221 tatctcgcgc aatattctac acctgagcgc gcaatccgaa tagacgcgcg cgagtgcgaa
     2281 cgcggatatt tttagcgctt tttcttggtg gcgtctttga tggcttgctg agcttcggca
     2341 gccttctcgt atttcttcgt aaagcgctcg acgcggccgg ccgtatccag caatttctgc
     2401 ttgcccgtaa aaaacgggtg gcagttcgag cagatttcga cgcgcaggaa ctcgcccttc
     2461 atcgtcgatc gcgtggggaa tgtgtgccca caggcgcaat gtacggttac cggatggtag
     2521 tccgggtgaa tgttcggttt catggaagat ctcccgccgg ctggccggtc tgcgcggctc
     2581 ccggtcaatt gccgggacta cagtcgcgtc gcttggtcag cggacaattt tctatcatac
     2641 gtgattcgcg gcgccctgcc aacatgatat acatgtgggt cgggaattcg tttggatgcc
     2701 cgctggggcg caaacgattc acggcacaag cgaagcatta ttcgcccgga ggtgaaaaaa
     2761 tgacgatgca ctcgatcagg aaattcacgc aacggccttt actacttgtc tgcgccgcgc
     2821 tgcttctggc gtcttgcagc ttcgcgggat gggccttggc ccaggagcct gccgcgcacg
     2881 acaggattta cgtagtcacc cacgtggata ttgtgccccc caatactgcc gcaggaacga
     2941 aattagtgca gcaatatgtt gccgatagcc gtaaagataa gggttttgtg cgcatcgaag
     3001 caggagcgca aatctcgcgc gtcaaccaca tttcgatcgt cgaagtgtgg caaaacgaga
     3061 aggctttcga cgaacatgtt gcagcggcgc acacaaaaga attccgtcgg caaattgatc
     3121 cgatgttggg cagcccgtat gacgaacgat tacacctgag cctggaatag aaataatttt
     3181 gagattgcaa gatgcgatat gcgaaaagtg gttcattctg aagacgcgtg tttatcagag
     3241 agaaaaaagc taggttataa aagcgcagcc aattcttgtg gcgccggaat ccgcttgggg
     3301 ttgaattgcc cggtaaacgc ggcgcaaatt gacgcgtacg ggcggcagga ttgggacgtg
     3361 aagcacagca tgaaacgctt cagcgaattc gtcaggattt aaactttcca gacaaatacc
     3421 gccatgaccc aaatccaata cgcgtgcttt tcgttccgga ttttcgccga tcaacgcgta
     3481 cgcattcgta ttggccatgg aaaagcgctc ccaccgccgc cgatccgtgg ttttctttgt
     3541 ttcgctttct gttgagggct ccacgtacgg caaattctga agcggcccgg caggcgtaat
     3601 tctttgattt ctctccgtcg gaccggtgcg tgaacctgct ccggcagcag ccttgctctc
     3661 gttcttgagc tgataaagcc tagcgtcggc gacgcggatc aaggcctcct gtaactcgcc
     3721 atcatccgga taaacagcca gaccataatc cagagccaga ggaattccta aattcatcgg
     3781 ttccagagaa ctggcatagg tggagcgcaa acggcgactc aaggttgccg cctgctccgt
     3841 gtcggattgc ggcagcaaga gcgcgaattc gtctccgcca atccgaaacg catagtcaga
     3901 tgtacgtaaa gatttccgaa gcgtcgctgc ggccatttga agcagcgcgt ctccctgttg
     3961 atgtccaaac ttgtcattca catctttgaa tcgatgcagg tctaacatga ccaatgccag
     4021 cttgtggttg tagcggcttg accggttcaa ttctttttca aaataatcat cgaagaagcg
     4081 cctgttgtag aggctcgtta atggatcggt cgtggcattg aattgaagtt tgcggagttc
     4141 ctcgtattcc atcaagatcg gcattcgcaa ctgcccgttg gatgccagca cttctgctag
     4201 cgctgcttgc agtgtcaccc tctttcccag gctttgcgaa agctcatttt tccgcgctag
     4261 cgtctgttcc caataatcca ggctctgcgt ttccgttaat tcaatttgcg caatcgaccg
     4321 gaaaaatcgc tgcagaaact gtccgcggcc ggacgggtcc atcgtggcga gcgtgtccat
     4381 caacagctcg aggaaagact cctcggcaga ccaacgtggt gggttgggag caccagtatt
     4441 cgccattgaa atgaaccatg aatttcaaga ataccccagc gcgggacggg cgtggagatc
     4501 gaattcgtag attacgtgca cgggaactgc cataatccat gcattgccct gaaaaatcga
     4561 ttgcctgtaa tgcattgagt cttagagatt aaaaagtttc gaacctgcag gaagcgcaaa
     4621 tacctgtgaa cacatggggt caatgcgcaa gagtttgcgt actctttggg aatgtttctt
     4681 cacgccatcc ccttcgttcc gatgaccttt accccagcga tttcacaaat tccacgccca
     4741 ggagatgaat tccatccgcg gcccggtgac gccgaactcg cgcctgaatc ggtggagtat
     4801 ctgggggtaa tgtcgcggaa ggagaggacg gagcaggaat cgttagattc accagctcat
     4861 tcattcgcac ggcgcgtgaa ctggcaaggt gtgcccccgt ggcacttata tcaagcgttt
     4921 taatcaaatc gagaaattca agaccggcag catcggtccc ccgaacaaac atcgggatct
     4981 gaacgtgaat tcgctgcgag cgacgacgct cgaaggtgga agtggacatg ctttctcttt
     5041 ctgacaaaag tcttgggaaa tcactttccc acagtcaaaa aactagtact tccgaactag
     5101 taccaacaat acccaagcac acgcattgcc agcgcagccg caaattcgtc tgttttcaag
     5161 tcattcattt acatacactt ataagcactt tttagggacg agaattcgtt ttccacagtt
     5221 cgtccacgcc catggtcgaa cgctttacta caattgtgca aaatagaacc cctttaccat
     5281 tgacagggta gtcgggtttt gacagtatcc gtttggtttc ccgcgtataa accgaagtga
     5341 atcaaatgga ataaaccgct ttcccaaaaa gcggataagt gtcccgagga gagcttggtg
     5401 agacgcggtc ctgtaatcga gttgccgacg agtgaagcga ttcctgcaag tgttgtcgca
     5461 agtctcctcg acttgctcga acagcctatt cttgtctctg accggtttgg gcggattctc
     5521 caagccaacg tcttagggaa gcagcgcctg ggatctctcg gattctcttc tgagtcaaat
     5581 ctaaatcttt ttgtcgatct attgcggatc gatcccaaat tgattgctaa caggatcgac
     5641 agaggcgagc aacaaatcga ttcgagtttt gcttgttgcg gcggcgccgc tatggctcgc
     5701 attcaatggc tcccggaagc ggactggctc gtagtacgaa ttgaagaagc ggaaaagcgt
     5761 ccgctccagt ttacccagaa tgatgcaaca gccacgaaac ataccgttca ggaattactg
     5821 caggaacgcg agattaccta ccgcaatttg ctggccgcat acttgaggct gcaagaagtg
     5881 aatcgccaaa aaactgtttt cctggcatcc gccgcgcatg aattaaaaac gcctcttgcc
     5941 gtaatcaagg gctattacga tttgctgctt tccggctcgc tcggcaaatt gactgacaaa
     6001 cagcgcgata ttctgcagga atcaaaggaa agttgcgagc gtctggttcg actggtttcc
     6061 atgttcctga attattcggc gctggagagc ggcaaactgg ttttgcactt gcgcgagaat
     6121 gatttgaggg attgcttgag cgaattggcc acgcgctggc acgaagcgtt tgtgcgcaag
     6181 catgtgcgcc tcgaaacaat tttgaacgcg gatatcccca ttttcaaatt cgactatcaa
     6241 aaagttcagc agacggtcgc gaatttactg gataactcgt tgaagcacac acccgccggc
     6301 ggcaatgtga tcttccgcgc ggaactttat ttctgggaac gccgcacggc cgaggaaatt
     6361 caaacgttgg accgacgccg ggaacgcacc cagtatccga attgcatcaa agtttccgtc
     6421 tcggattccg gtccgggcat cgcctccgaa catcaccagg aaatattcga agatttcgtg
     6481 cgcgtggacc gcacgtcttc gggcatgggt ttgggactgg caattgccaa gcgcctggtg
     6541 caggcacatc gcggcaagat ttgggtggaa agcgaaccgc ggcgagggtg cacttttacg
     6601 ttccttttgc cgatagacaa tacctagtgg aatttgggct aaacgaaggt ggggcaaatg
     6661 gctgaaaagg aaagaatcct ggtagtggac gatgaaccca gcatacgaaa gtatttgggt
     6721 acgttgctcg aggtcgacgg ataccaagtc gaggccgtga attccggctt ggaggcgatt
     6781 cagaagatcg aaagcggcga tcgtcccgac ttcatcattc tcgacgtgtt gatgcctgag
     6841 ttgaacggca tcgaaactct gaaacagctg atgcagattg accgttcgct gaacgtcgtc
     6901 atgctttcct gctccaatga ggtaggcacc gtggtcgaag ccatccggct cggcgcacag
     6961 gattacctga ccaagccctt tgaaaaagcg gaactcgacg tggcgatgct gaagtgcagg
     7021 cagaagcagc aactgcgcca ggaaaacaaa gcgttgcgcg agtactgcga cacgattacg
     7081 gaagacctgt gcttcctcgc cgccagcccg cagatggtgc gaattcgtca gcagattctt
     7141 caaatcgctc ccgtcgatgt accagtcttc atttatggtg aaagcggagt tggaaaagag
     7201 gtggttgcgc gcttgattca catgcgatca aaccgccgcc agcaagcttt catcaaagtg
     7261 aattgcgctg cgctcccggg cgaactgctt gagtcggaat tgtttggctt cgaacaaggc
     7321 gcctttaccg gagccgtgcg atcgaagccc ggaaaatttg aactggccac caagggaacc
     7381 attttcctcg atgaaatcgc ggaaatgagc ccgcatctgc aagcgaaact cctgcacgtc
     7441 cttcaggatc atcagttttc gcggcttggt ggacgccagt tgatagacgt cgatgtccgc
     7501 gtcctggccg caaccaacgt ggaaattcaa gaggcgatga agtcgggacg cctccgcgaa
     7561 gacttgtact accggttgaa cgtgctttcg atcaacgttc ccccgctgcg cgaacgcgct
     7621 tcggaaattc cgctgctttt ccgccatttc ctacagaaat acagcgagaa attcagcaag
     7681 gagcccatgg ttccttcgca gcatttgatg gaagcggcgg tccgttatcc atggccgggc
     7741 aatcttcgtg agctcgaaaa tttcgtgaaa cgttacgtta ttctggaaga cgacgagggc
     7801 agcttccggg agctcttgga aatgtccgcc acgcgtcagc gaatttctcc tcgcgaggaa
     7861 gccgctccgg tgcgcgaaca aggattgaag gcgctcgtgc gcagcttgaa ggacgaagct
     7921 gaaatggaag cgattggcga tgccctcgaa aagacgcatt ggtgccgtaa agacgccgcc
     7981 aagatgctag ggatcagtta caaggcgctg ctctacaaaa ttcgccaatt cggactggat
     8041 accggacgac cagctcgcgc gagcactaag aaaggtgcgg cggggagcac aactcccgga
     8101 cctgcgccat cgaaccttgc gcccgctggc cggcatagct agtgcgacta gtacatgcaa
     8161 aggtttgatc atagtaagga tcaagctttc caatatttcc cttgagacgt ttttttcagg
     8221 ggtcgcaaag caacgggtga gcgaacagtt ttgctgagtt cggaattttt cgcctcattt
     8281 aatgggtatt catcgggcgc aagccgattg acctgttctc tttcggagtc aggttcatat
     8341 ttgcgaacgt gacaagggat tatttttcat aggaatcatg gggttacagt attgcttttg
     8401 aagactcaag catgtattcc tgattgatca ctggttaaat tcagtcataa tgttggcagg
     8461 gccaccgctt tggaatgtca attgctcctt gttaatggtc gaattgggag tacatggtag
     8521 agacagagct ttggggggaa caaaaagatg acggagcgga gaactgcacg ccgatatgac
     8581 ctgtccttgc cggtcattat ccgtgtgccg gtggagaagg aaatagcctc acgaagcgga
     8641 aaaacgcgcg atatttcaac gcgcggggtt tactttacaa ttgatcagga tttgggcacc
     8701 ggcgcagagc tcgatattac tttgacttta ccttccgagg taactcgcgg atccgaagta
     8761 ttcatccggg ccatgggtaa agtggttcga attgacaaaa agccggatag cgagacctct
     8821 cgcgtcggcg ttgccgcagt gatcgagcga tacgaaatta tccgaacaga gccttccact
     8881 cattaagcga tctcttttta aagacatcca ctacgccctg aggtaagttc cccccaggga
     8941 tttttgaatt tggttgaaat cattgcatcc gacatcgctg tccctctaga agcgccagcc
     9001 tcaactattg ttagcgatca gatccacgga ttttttagtc tccgagcccc atcccagccc
     9061 ggcttcgtgg acgaattcaa cggcagacga tataatcccg cccctggaga agttgtgcct
     9121 gccgcgaagt ccatgtggcg ccaccgcaaa tgaattcgcc aa
//
LOCUS       DASZMB010000027         4240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_20137, whole genome shotgun sequence.
ACCESSION   DASZMB010000027 DASZMB010000000
VERSION     DASZMB010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4240
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20137"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="VG272_01035"
     CDS             <1..1012
                     /locus_tag="VG272_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWF12291.1"
                     /translation="DLKYPDELATRLQMQIQQVFETKTGLIDETEYRSPTGAGGYYEY
                     ILRPVFNREGLVEFVAGSTRDITERKRVEGELRETHARLSDLAASLESQVQARTSELE
                     IRTNDVLLQSEQLRELSLRLITTQDEERRRIAREMHDSAGQTMAMLSMNLAQLCSQLD
                     GTNPKLSETANQARDFARELEREIRTTSYLLHPPMLDELGLRAALFWYVEGLTQRGAI
                     QVDLRVPEFDRLRADLELTIFRIVQECLTNIHRHSKSKTAQINIGIDGAMFVIEVRDF
                     GEGITAQNLDKIRKGAGGVGLRGIRERLRPYAGEIKIDSEKGSGTIIKITLPYQRTTS
                     KS"
     gene            1207..1839
                     /gene="mtgA"
                     /locus_tag="VG272_01040"
     CDS             1207..1839
                     /gene="mtgA"
                     /locus_tag="VG272_01040"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HWF12292.1"
                     /translation="MLAVAFLWTIAALILVSARWIDPPTTAVHMQRRAQSWMNRKPYR
                     ERHDFIPLNQISADLQHAVIAAEDARFYQHHGFDWHQIEIAAEDDLEGERTRGASTIT
                     QQLVKNLFFGTSRSFLRKGAEVALVPVAEFALGKRRILEIYLNVVEWGPGIYGAESAC
                     RYYDQTSAHNIGRTQAARLAAILPAPLKRRPERMNSYSAIILKRMSQMGW"
     gene            complement(1907..2497)
                     /locus_tag="VG272_01045"
     CDS             complement(1907..2497)
                     /locus_tag="VG272_01045"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HWF12293.1"
                     /translation="MPIDFSNAWKSGSRIINFIISLLPNIALGLVILIAFLFLASAAK
                     SLVRRLVKNRGRRANLGLLLGQLTQATVAIFGFLVALSAVAPSFHASDLIKMLGVGSV
                     AIGFAFQNILQNFLAGILLLFQEPFLVGDWISVTGLEGRVDDIQARATLITDSNSKKI
                     VIPNAVLFTNPVTVGEGKRGEENKEAPPAQPAAKST"
     gene            2735..2959
                     /locus_tag="VG272_01050"
     CDS             2735..2959
                     /locus_tag="VG272_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12294.1"
                     /translation="MDTREYQWRAHFVAALTKVDEEDSLARLAIADDAIFLRLLELEG
                     TTGNDEERLALEDTMQDIRMLRDRSNHFRS"
     gene            complement(3062..3934)
                     /locus_tag="VG272_01055"
     CDS             complement(3062..3934)
                     /locus_tag="VG272_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12295.1"
                     /translation="MSSNCGITCVGLVWKRLLMCAIAMAAAAASISAQQQGNSQYSPR
                     FGMGHLPDATVVHGSDVESSAIADDARCFPWKLSEVRAGTVDVQRLNIPSSAKHEYEK
                     ACEASKKNKPDEVEQHARAAIEKIEKYPAAWVMLSLSLAEQQKLDDAQEACAHAMKAD
                     PAYLPAYLCSAEVSTRKAEWKQVLTASSMAISLNLGGDSYAFYYRATAYLHLNNLDEA
                     KKNALQAAQIDVNHSEPSIDLLMAQIYEREGDKAQAIAQLQLLLKRRPDRQTADAANQ
                     RLAALESQKPATAQ"
BASE COUNT          998 a         1176 c         1109 g          957 t
ORIGIN      
        1 cgatttaaag taccccgacg aacttgccac acgcctgcaa atgcagattc agcaagtttt
       61 tgaaacgaag acagggttga tcgacgaaac ggaataccga agcccaactg gcgccggggg
      121 atattacgag tacatcctca ggccggtttt caaccgcgaa gggctggtcg agttcgtggc
      181 gggatcgact cgcgacatca cagagcgcaa acgcgttgag ggggagcttc gagaaactca
      241 cgcgcgcctt agcgatctgg cagcatcgct ggagagccag gtccaggctc gcaccagtga
      301 gctggaaatc cgcacaaatg acgtgctctt gcaatccgaa caacttcgcg aactctccct
      361 gcgcctcata accacacagg acgaagaacg ccgtcgtatt gcccgcgaaa tgcatgacag
      421 cgcaggccag acgatggcga tgttgtccat gaacctcgcg cagctttgca gccaacttga
      481 cggcacgaat ccgaaactaa gcgagaccgc gaatcaggcc agagacttcg ccagagaatt
      541 ggagcgggaa attcgcacga cttcctacct gctacatccg ccgatgctgg atgagcttgg
      601 tcttcgtgcg gcattatttt ggtacgtgga aggcttgacg cagcggggcg caattcaagt
      661 ggatctgcgc gtgccggaat tcgatcggct gcgtgccgat ctggaattaa caatttttcg
      721 catcgtacag gaatgcctga ccaacatcca ccggcactcc aaaagtaaaa cagctcaaat
      781 caatattggc atcgatggcg ccatgttcgt gatcgaagtt cgcgacttcg gtgaagggat
      841 cacggctcag aatctcgata aaattcggaa aggcgcaggc ggcgttggat tgcgcgggat
      901 tcgcgaacga ctccggcctt atgccgggga aataaaaatc gattctgaaa aaggcagcgg
      961 caccatcata aagatcactc tcccctatca acgtacaacc tccaagtcct gacagcccgt
     1021 ttctcgctta caactaacgc gccaaaaata atgtgaattg ctatagacaa ggacacgtgc
     1081 ttcaacatcg tctctcgccg gcaactctac ccggtctatc attttgaatt gtgactgcaa
     1141 cagcggacgc ccaaaccaaa gctcgatccc gaaagggctt gctgcgatcc ttttttttgg
     1201 tggctcatcc ttgccgtcgc gtttctatgg acgattgccg cactgatttt ggtgagcgct
     1261 cgatggatcg atccgcccac aaccgccgtg cacatgcagc gccgcgcaca gtcctggatg
     1321 aaccgtaagc cgtatcgcga acgccacgat ttcattcctc tcaaccaaat ctcggcggat
     1381 ctgcagcacg ctgtgattgc ggcggaagac gcacgcttct accagcatca tggattcgac
     1441 tggcatcaaa tcgagatcgc cgccgaagac gatctggaag gcgagcgcac gcgtggggcc
     1501 tccaccatta cacagcaact cgtaaaaaat ttattttttg gaactagccg ctcgttcctc
     1561 cgcaaaggtg cggaggtggc gctcgtgccg gtcgcggaat tcgccctcgg caagcggcgt
     1621 attctggaga tttacctcaa tgtggtcgag tggggccccg gaatttacgg cgcggaatcg
     1681 gcgtgccgtt attatgacca aacgtcagcg cacaacattg gccggacaca ggcggcacgg
     1741 ctcgccgcca ttctgcccgc cccattaaaa cggcgacccg agcgcatgaa tagctacagc
     1801 gcgatcatcc tgaagcggat gagccaaatg ggctggtaac cgagtcgctg cgtgcacgtt
     1861 ccaaccccaa gcaacagtgc gtgtgctacc tgaaaccggc aatcaattat gtagacttcg
     1921 cggcaggctg cgcgggcggc gcttctttat tttcttcgcc ccgcttgccc tcccctaccg
     1981 tgacgggatt ggtgaacagg acagcattgg gaattacaat tttttttgaa ttggaatccg
     2041 taatgagcgt cgcgcgagcc tgaatgtcat ccacccgtcc ctcaagccca gtgacggaga
     2101 tccagtcgcc caccaggaac ggttcctgaa acaacaacaa aattcccgct agaaaatttt
     2161 gcagaatgtt ttgaaacgcg aagccgattg ccacgctgcc gactcccaac atcttgatta
     2221 aatcactggc atggaaagac ggagccaccg cggatagcgc aactaaaaaa ccgaaaatgg
     2281 ccacggtggc ctgcgtaagc tgccccagga gtaatcccag attagcccgc cttccgcgat
     2341 ttttcacgag gcgccgcaca agggattttg ccgctgacgc caggaagaga aaagcaatga
     2401 ggatgacgag accgagggcg atattgggca aaagcgaaat tataaaattt ataattctgc
     2461 ttccgctttt ccatgcatta gaaaaatcga ttggcatggc gcccaccaca gattgtatcg
     2521 ttgcgtggat gccaagcaat cccgatttcc ctgtatacca aacccgtaaa cttcttattt
     2581 cagtgggctg ctcgtcagac tgcgaggcgt ctaagcataa cttcgccggg tattacattc
     2641 taggccctat gttcgatttc gtcggacgtc gcatacgcta ggctcggcga catgtcaccg
     2701 tcgccggtgg attgctactt ttggaggggt acctatggac acccgagagt accaatggcg
     2761 tgctcacttt gtggccgcct taacgaaagt cgatgaggaa gattcattgg ccagacttgc
     2821 catcgcggac gacgctattt ttttaagatt gttggaattg gaaggcacaa ccggtaacga
     2881 cgaagaacgt ctcgctctcg aagataccat gcaggacatc cgcatgctga gagaccgcag
     2941 caaccacttt agaagttaac attgtgacgc atttttcgca cggaagcatt gcttccatcg
     3001 aaatgaggcg gaacatttta aaaggcatgc caacaatttt cggccttccg tcgcccaatt
     3061 cctactgagc ggtggcgggt ttctgggact cgagcgctgc gagtcgctgg ttggctgcgt
     3121 cggcagtttg ccggtcaggg cggcgcttca aaagtagttg gagttgtgcg attgcctgtg
     3181 ctttgtcgcc ttcgcgttca tagatttgag ccatcaatag atcaatcgac ggctcgctgt
     3241 gattcacgtc aatttgcgcc gcttgcagag cgttcttttt ggcttcgtca agattgttca
     3301 gatgcagata ggcggtggcc cggtaataaa aggcgtacga gtcaccgccc aagttgaggc
     3361 tgatcgccat gctcgatgca gtgagcacct gtttccactc cgcctttcgc gtggaaactt
     3421 ccgccgaaca aagatacgcc ggcaagtaag cgggatcagc tttcatagcg tgggcgcatg
     3481 cctcttgcgc atcatccagt ttctgttgtt ccgccagact gaggcttagc atcacccacg
     3541 ccgctgggta tttttcaatt ttttcgatgg ctgcgcgcgc atgctgctcc acctcgtccg
     3601 gtttgttttt cttcgacgcc tcgcatgctt tttcatactc atgcttcgcg ctcgacggaa
     3661 tgttcagcct ctggacatcc accgttccag cccgcacttc cgacaacttc cacggaaaac
     3721 atcttgcgtc atcggcgatt gcgctcgact ccacatctga gccatggacg acggtggcat
     3781 caggcagatg gcccattccg aaccgcggcg aatactggct attgccttgc tgctgcgcag
     3841 aaatggatgc ggccgccgca gccatcgcga tcgcgcacat cagcagccgc ttccacacaa
     3901 gacccacaca agtaattccg cagttcgagc tcatattgaa ttttcccctg atgcatcgat
     3961 aggctgagga tcgaaacctt agtgggccga ctacgtgcag gtttccttaa cccggatgct
     4021 aagccttacg tccagatcga gtcaaacgga ttcggcgcgt gcattgacgt ttcctcattc
     4081 ccgcatcgcg tcgaacagaa tgcaacgtct ccgttgattt tcagctgaat gttcgttgac
     4141 gcaggaaaat cccggtagta taaggccctc tcgaaggagg acgcgtgcaa tgataaaaat
     4201 tcgattggta tgtctgttgt tgctgtcagg aatgctgctg
//
LOCUS       DASZMB010000028         4546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1408245, whole genome shotgun sequence.
ACCESSION   DASZMB010000028 DASZMB010000000
VERSION     DASZMB010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408245"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VG272_01060"
     CDS             complement(<1..578)
                     /locus_tag="VG272_01060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF12296.1"
                     /translation="MARTDVQLMLDVKAGDEASFELLLRKYRTPLVNFLYRMVRDSAA
                     AEDLAQEVFLRVYRARKEYAPSAKFTTWMFRIATNLALNSVRDNRHRQMEISMDQPFE
                     TGEEELHAIEVPDRAPTVEQVLVARNRSEMILRAIHALPEKQRAAVLLHKYQELDYDE
                     IARILECSESALKSLLFRAYETLRVELAPLVNS"
     gene            complement(939..2072)
                     /gene="ychF"
                     /locus_tag="VG272_01065"
     CDS             complement(939..2072)
                     /gene="ychF"
                     /locus_tag="VG272_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HWF12297.1"
                     /translation="MATRGPEARRLPVCYDLQLMQTGIIGLPQVGKTTLFKILTRAQL
                     DEKAARGATHVGVARVPEPRLEQLAKLYNPKKITYATVEYVDVGGMVKDRTKDSAVLA
                     PLREVDALAHVLRVFDDPAVAHSAGSIDPLRDAEVLDLELMLSDLDQITRRIERVEKD
                     LKKKREPILEQELVLLNRCKTAIESEKPLRELEFNPEERKMINGYKFLSQRPMLYVLN
                     LGDSEVAELDTAVERHNLSKLVGRPNTAIVSICGRIEAELAELDPAEGAEMLAAYGLK
                     SSGLDRLIQATYHLMGLTSFFTAGEPEVRAWTIKRGMSAQKAAGAIHSDIERGFIRAE
                     VVRWDDLLAAGSLPAAREKAQVRLEGKEYVVQEGDVILFRHSG"
     gene            2120..3625
                     /locus_tag="VG272_01070"
     CDS             2120..3625
                     /locus_tag="VG272_01070"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HWF12298.1"
                     /translation="MKNSFSAFQQKLIDIAQLKSAFAVLNWDKDVFMPPKGSGPRAAM
                     LGYLAGELHDKVLSQEFRDLLREAKAASEANELSAGDRAIVREVWRDVNREEKLPVEF
                     VKELAQVTSEAYHVWMNARKQKDFKLFAPHLTKIVALKRREAELIGYKQSPYDALLDT
                     FEPYATTEEITVALGDLRNFLAPFLVKIKKSKAKITRDAIAGAFDIEKQKAFCTMAAK
                     QIGYDFDAGRLDVSAHPFSTTFHPLDSRITTRYDSSEFMQSISGVIHETGHGLYEQGL
                     PAAHFGTPLAEAVSHGIHESQSRLWENLVGKSRPFWAFFYPLLQNEFPTPLQKVSLDS
                     FYRSINTVEPSFIRVEADEVTYNLHIIVRFEIEIALVKGDIAVEDLPQIWNSKMKDLL
                     GLDVPDDSLGVLQDVHWSGGMIGYFPTYSLGNLYSAQFFAAARRDIPTLEDEMSHGEF
                     SKLLKWLREKIHVHGKYYSAQELAKNATGEKLNSKYFAEYISRKYGEIYEL"
     gene            3825..4445
                     /locus_tag="VG272_01075"
     CDS             3825..4445
                     /locus_tag="VG272_01075"
                     /inference="COORDINATES: protein motif:HMM:NF027116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HWF12299.1"
                     /translation="MVSCSGASAQTNPSSQSIASDSPLSLSAQSLNFGNVNLGLTNSL
                     QVNIANTASQNITLSQQSVSGEGFSTEGIGSGVTLNPTQSATLKINFRPDITGNMTGS
                     VVLSSDGTSAPMVITLTGNGTPNEHSVTLNWTSSMSAAVGYNIYRESSSDGPWVRLNV
                     SIDAGLSYTDPTVQNGQSYLYAVSTVSPDDFESALSDPIPVLIPAT"
BASE COUNT         1013 a         1272 c         1217 g         1044 t
ORIGIN      
        1 gaattcacca gcggcgccag ctcaacgcgc aaagtttcat atgcgcggaa gagcagcgat
       61 ttcagcgcgc tttccgaaca ctccagaatc cgggcgattt cgtcataatc aagctcctga
      121 tatttgtgca gaagcacggc cgcccgttgc ttttccggca gcgcgtgaat ggcgcggaga
      181 atcatctctg agcggttgcg ggcaaccaat acctgctcca cggtgggcgc acggtccggc
      241 acttcgatcg cgtgtagctc ctcttcgccc gtctcgaacg gctgatccat ggaaatctcc
      301 atttggcgat ggcgattgtc gcgaaccgaa ttcagcgcca gatttgtggc gatgcgaaac
      361 atccaggtgg tgaacttcgc gctcggagcg tattcctttc gggcgcgata aacgcggaga
      421 aacacttcct gagctaagtc ttcggcagcg gcggagtccc gcaccatgcg atacagaaaa
      481 ttgacaagcg gcgtcctgta cttgcggagc aaaagctcaa aggacgcttc gtccccagct
      541 ttcacgtcca gcatcagttg gacgtcagtc cttgccatgg cctccacgct ctattcaacc
      601 cgtgtcgtgt caaaaagttg cggaaagccg gatggcggct gtcccgccga gcacggatca
      661 aattatgtta cagcgattgt atatgatcga aaggtggggc ggataccatt tgggagagcg
      721 ttgtggctgt tcggcaactg aatttaactg catgctgcgc tatcattcag ccgcgacgtt
      781 cagtttcgcg ggaagcgagc ttcagtcccc taagcttcag gcggaaaagc ttaaaagcct
      841 ctctgctatt ccaccgccta tgggcttaag cccacacctc ggaaactgca aaccccagcc
      901 cgggcctttc tccggcgggc tcacgtagag cccgcaccct atccgctatg gcggaacagg
      961 atgacgtcgc cttcctgaac gacgtactcc ttgccttcca ggcgaacctg cgccttttcg
     1021 cgcgctgccg gcagacttcc cgcagcgagg agatcgtccc agcgaaccac ctcggcgcga
     1081 atgaagccgc gttcaatatc cgaatggatc gcaccggctg ctttttgcgc gctcatgccg
     1141 cgcttgatcg tccacgcgcg cacttcaggc tcgccagcgg tgaaaaatga agtcaggccc
     1201 atcaggtgat atgtcgcctg gatcagccgg tcgaggccgg aggacttgag gccgtacgcc
     1261 gcgagcattt cggctccctc ggcaggatcg agttcggcga gctcggcttc gatgcggccg
     1321 caaatggaaa cgatggcggt atttggccgg cctacaagtt ttgagaggtt gtggcgctct
     1381 acggcggtgt cgagttcggc gacttcgctg tctcccaggt tcaagacgta cagcatcggc
     1441 ctttgcgaga gaaacttgta accgttgatc attttgcgtt cttcaggatt gaactccaac
     1501 tcgcgcaggg gcttttccga ttcgatggcc gttttgcagc gattcagcag gaccagttcc
     1561 tgttcgagaa tcggttctct cttctttttc aaatcctttt ccacgcgttc gattcggcgg
     1621 gtaatctgat cgagatcgga gagcattagt tccagatcga gcacctcggc atcacgcaac
     1681 ggatcgatcg aacccgcgga gtgggctacg gcgggatcgt cgaaaacgcg cagcacgtgc
     1741 gccagcgcgt ccacttctcg caagggtgcg agcacggcgg aatccttcgt gcgatccttc
     1801 accattccgc ccacgtcaac gtattcaacc gtggcgtaag tgattttttt ggggttgtag
     1861 agcttggcga gttgctccag gcgcggttcg ggcactcgcg ccacgcctac gtgcgtggcg
     1921 ccgcgcgcag ccttttcgtc cagctgtgct cgcgtcaaaa ttttgaagag agtcgtcttg
     1981 ccgacttgcg gaagcccgat gatcccggtt tgcataagct gcaaatcgta acataccggt
     2041 aggcggcgcg cctcaggacc tcgcgtggcc acaataaaaa ttgcgcacgg attctgctaa
     2101 actgccgtgg gtgacgatca tgaaaaactc tttctcggct tttcagcaga aacttatcga
     2161 catcgcgcaa ttgaaatccg catttgccgt cctgaactgg gacaaggacg tgtttatgcc
     2221 gcccaaaggc agcgggcctc gtgctgcgat gctgggatat ctggcgggcg aactgcacga
     2281 caaagtgctg tcacaggaat ttcgcgacct gctgcgcgag gcaaaggccg cctcggaggc
     2341 gaatgaattg tccgccggcg accgtgcgat cgtgcgcgag gtatggcgag acgtcaatcg
     2401 agaagagaaa ctcccggttg agttcgtcaa ggagctcgcg caggttacct ccgaggccta
     2461 tcacgtctgg atgaatgccc ggaagcaaaa ggatttcaag ctgttcgcgc cgcatctgac
     2521 gaaaattgtc gcgctaaaaa ggcgcgaggc cgaactgatt ggatacaagc agtcacctta
     2581 tgacgcgctt ctggacactt tcgaacccta tgcgacgacc gaggaaatta ctgtcgcgct
     2641 gggtgatctc cgaaattttc tcgcgccatt ccttgtgaag attaaaaaat cgaaagcgaa
     2701 gataacccgc gacgccatcg ccggcgcctt cgacattgaa aaacagaaag cattttgcac
     2761 catggccgcg aagcagatcg gctatgattt cgacgccggc agactcgacg tcagcgccca
     2821 cccgttcagc accacctttc acccactgga ttcgcgtatc accacgcgtt atgactcctc
     2881 cgaattcatg caatccatta gcggcgtgat tcatgaaacc ggacatgggc tatatgagca
     2941 aggtttgccc gccgcacatt tcggtacgcc gcttgcggag gcggtttccc atggcattca
     3001 cgagtcgcaa tcgcggctct gggagaatct ggttggcaag agccggccgt tttgggcttt
     3061 tttctatccc ttgcttcaaa acgagtttcc cacgccgctg cagaaagttt cgctcgacag
     3121 tttctaccgt tcgatcaata cggtggagcc gtcattcatc cgcgtcgagg ccgacgaggt
     3181 cacctataat cttcacatca tcgtgcgatt cgagattgaa attgctttgg taaaaggcga
     3241 catcgccgtt gaggatcttc cgcaaatctg gaattccaag atgaaggacc ttctggggct
     3301 cgatgttccg gacgattcac tgggcgtgtt gcaggacgtc cattggtcgg gcggaatgat
     3361 cggctatttc cctacgtatt cgctgggaaa tctatattcg gcgcaatttt tcgctgctgc
     3421 ccggcgcgat attcctacgc tggaagatga aatgtcgcat ggtgaattca gcaagctcct
     3481 caagtggctc cgcgagaaaa tccacgttca cggaaagtat tattccgccc aggagctagc
     3541 caaaaatgcc acgggcgaaa agctcaacag caaatatttc gctgagtaca tctccaggaa
     3601 atacggcgag atttacgagt tatagcgccg gccgcgatat ccattcaatg tagtgcattt
     3661 caaggacagg gtagctcctg taccacgact gaaccgcggt acagtacttc tcaacatcag
     3721 ttcgccataa aattcggcgg ttgtaaatct cagttgtaaa catacgaggt gatccaatcg
     3781 cacgaatctc tgttgttgcg ttgctgttaa tctctgcagt ttccatggtt agttgctcgg
     3841 gcgcatccgc ccaaactaac ccatcctccc aaagtatcgc ttctgactcc cctttgtcac
     3901 tcagcgcaca aagcctgaat tttggcaatg taaacttagg actgacgaat agccttcaag
     3961 taaatatcgc aaatactgcg agccagaaca tcaccctctc gcagcagtcg gtcagcggcg
     4021 aaggctttag tactgagggc atcggatccg gcgtaacttt gaacccgact caatccgcga
     4081 cactcaaaat taacttccgc ccggatatca ccggaaatat gacgggaagc gtagttctct
     4141 cgagcgatgg aaccagcgcc ccgatggtga tcactctgac gggcaatggg acacccaatg
     4201 agcattccgt tacgttgaat tggacttcga gcatgtcagc cgcagttggc tacaacatat
     4261 atcgggagtc ttcatcggac ggtccgtggg tgcggttaaa tgtatctata gacgcgggcc
     4321 tctcctacac cgatcccacc gtgcaaaacg ggcaatccta tctgtatgcc gtaagtaccg
     4381 tcagtccaga cgacttcgaa agcgcgcttt cggaccctat tcctgttttg attcctgcga
     4441 cataggagtc aaatcgaagt tttcgcaagg gagtggtttc aatgctcggt gatgcaatcc
     4501 tgtctgtaaa cgattaagcc caccgatccc ttattttctt cagcgt
//
LOCUS       DASZMB010000029        34861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2227549, whole genome shotgun sequence.
ACCESSION   DASZMB010000029 DASZMB010000000
VERSION     DASZMB010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 34861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34861
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2227549"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VG272_01080"
     CDS             <1..849
                     /locus_tag="VG272_01080"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF12300.1"
                     /translation="NNLAATYHDQSMNLQAEQSYKRAMSILEKSPNQNVSMGRTLNNL
                     ASLYQDMGADAKAEPLYNRAMGIWKNLAKKDNAGEAFTAISLAGIYHTRNQDEVAEPL
                     YLRALTLWNAEGKADSEIAADAFIHLAGIYRFRSDNESAASMYARVIAIWEKTGDIDK
                     SEGIAAQAALGEIYRSEGKYEQAEPLLEGALKGTEKMVGSDDLQVAASLNNLAVLFYC
                     EGNYQAAEPLYKRVLDIRQKNLGADNPMALQTVEIYATLLRHEGKQVDAAKLEAQAAR
                     AGVAHL"
     gene            1301..2803
                     /locus_tag="VG272_01085"
     CDS             1301..2803
                     /locus_tag="VG272_01085"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWF12301.1"
                     /translation="MGATNLKTIQYSGDGWSSMIGQTYGLAEDWPHYEVSGYTRVIDY
                     DAKWSREDYTRRQGKYPLLGRVPMPEQHMTSILSGTYAWDMQGDVPVPLTRLYLDGVT
                     YSDLRQLEIVLTPHGFLKAALAAKDATAISLPIIGASDFGLSQFGRKVTVVSFTMGKY
                     KVNGTINDKNLVELVDTWYPNPVYGDMDYEMRYTKYRDFGGIMFPGQVHVHQGDPRLN
                     PAHNYYEFNITDVKANVPVTPMPVPESVRTATLASVKVESQKLADGVWLIGGGTHNSV
                     LVEFKDFVAVVEAPQNEARSLAVIAEVNKLVPNKPIRYLVNTHHHMDHAGGLRTYLSQ
                     GTTIVTHESNKQYYLDIMFYPAPRELDPDRMAMFSPMYMISRRPAPIDTVGGDTRGEG
                     KYVVTDGEHVMEIFHVQDMGYELGDPSYNQGNHSADMLMAYLPKEKILINADLYSPAE
                     PGAQPPAATAAMKTLYQNMLKLKLDVVQHVPIHGHAGTNADFLKIFGKGQ"
     gene            3023..3946
                     /locus_tag="VG272_01090"
     CDS             3023..3946
                     /locus_tag="VG272_01090"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWF12302.1"
                     /translation="MSESAKIVEVKSPELLNLIDKNAVVEQICTGFWFTEGPIWNAKE
                     QCLYFSDMPGDVRRRWSATDGVKEVRKPSNKCNGMTYDGVGNLYVCEHVTSSLVMETP
                     SGERKVLASHWQGKELNSPNDVVVRSDNSVYFSDPSYGRMPVFGLERKQELDFQGLYR
                     ISPDGKLHLEANDFGQTNGLCFSPNERILYVNDSPRAHIRMFDVAADGSLSNSRIFAE
                     QIGDGILENGIVDGMKVDERGNIFVTGPRGIWVFSPTAEHLGVIRMPEHAGNLTFGGR
                     NWDELYCACSKSIYRLKMKVRGNPVAYMKMR"
     gene            3956..4567
                     /locus_tag="VG272_01095"
     CDS             3956..4567
                     /locus_tag="VG272_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HWF12303.1"
                     /translation="MAERKLNAKKTALIIQDLQNDVITDGGAFASSGAPAHAKKQNVV
                     ENVKTLAAAMRKAGAIVIHVHYIVEPGAPGLKLNAPLFRGLAESKAVVRGTWGAAPAE
                     GLEPQKGDILVEKMRMNGFHGTKLETILQGYGIDSLVITGAWTNFSIEHTARHGADAG
                     YDVVVATDGTSTVSDEWQNAGLNYALQNIADRLSCQEIIQALS"
     gene            4610..5650
                     /locus_tag="VG272_01100"
     CDS             4610..5650
                     /locus_tag="VG272_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HWF12304.1"
                     /translation="MTIPLETSIVVSAEDVRATIVHVLSRLGANERESSIQADVLTEA
                     DLRGHHSHGLQRLPVLAARIKKQLIRVKAEPDYIWTADSVLSVDGKDGLGPFVAETAH
                     EEAKSRLQQQGIVAVAIRNSSHIGMIGYYCEKRAHEGFVCIAMTESEALVHPHGGTKA
                     LVGTNPIAIGIPSEPSPFVFDMATSTSAIGKIYASKHRGENIPLGWAIDAEGNPTTNA
                     EAALKGSLTPLAGPKGYGLGISIGILAGLLPGAEIGRAVLGTLDTEFRCTKGDLFILM
                     NPAAFPGGPMLAAHVASYLDELRNSPPQKNCERVIVPGDRARKIREERMCSGIPLPKE
                     VWLAAERLKNEF"
     gene            5652..6845
                     /locus_tag="VG272_01105"
     CDS             5652..6845
                     /locus_tag="VG272_01105"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HWF12305.1"
                     /translation="MSNHVLGFSTAGRIFFGWGAASVLRDVAKEFGPRVFICTDRNMV
                     KAGVCEKVFANLAEGKAEVKIFDGGQPEVDRQTIETAAAAAQAYAPDVVLGLGGGSNL
                     DLSKAVALLVRYPGSLSSYYGENNVPGPIAPVVAVPTTAGTGSEVSPVAVVADAERAM
                     KIGIASRALIPKWAIVDPALTVSCPASVTAHSGMDALVHAIESYCARVRTDCSPHAIF
                     VGKNPVSDVLATKAITAITKFLPIAVGQPNNREAREQMALGSLLAGMAFSAAGTAAVH
                     ALQYPVGEATHTPHGLGNAVLLSTVMKRITSSRVEEMAFIARSLDSSLHSQTNAEAAA
                     KAAILVDEFASKVGIPKGLHTLGVKEAQLPELAKMATTVKRLLDNSPVSFDESDLLHL
                     LQDAF"
     gene            6992..8251
                     /locus_tag="VG272_01110"
     CDS             6992..8251
                     /locus_tag="VG272_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12306.1"
                     /translation="MSKKNGILAFGLAAGLCLLVLFSARSARTQNGPKYKVDAEWPKP
                     LPNKWKMGGVTGLAVDKDDNVWVHDRPNDLTDIELEAELNPPIADCCVRPPSMIHIDK
                     EGNVIGSIDPPQGHGMAVDSKGFIYLGNVVNGIGNVRKYDPKTGAFISELPHTPDMPP
                     GGGGGDAAPDNPRVPGHGSMGPVFGFLPGGRPKPNPAARAAREAATVAFRAKYPPTTP
                     MIVGGIEEVRLDEPAHELYAADNYLGGRVMVFDMDTFQFKRGWGAYGHKLSEITIDDK
                     DRAYVPNGPMPKEFRGHLTLNVSHDGLVYAADRMANRIDVTTKDGKFLKEFIMAPWTG
                     VGGSTGGVMFSPDKEQRFLYISDLTNNHIWFLNREDGKVVSTLGSMGQNAGQFYGLHM
                     ISVDSKGNIYTGEVFGGERVQRFVPVK"
     gene            8534..8866
                     /locus_tag="VG272_01115"
     CDS             8534..8866
                     /locus_tag="VG272_01115"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWF12307.1"
                     /translation="MAKKDRRMIQRFQAKPDDFILYDERSAPVRDVSMEGVFVLDPDP
                     LPVGSEIVFIFRAGHQDISLEGIVRHSVDQQGMGIHFTKIPAVSKRRLIIHIATLAPA
                     PFALDMPS"
     gene            8991..10448
                     /locus_tag="VG272_01120"
     CDS             8991..10448
                     /locus_tag="VG272_01120"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF12308.1"
                     /translation="MRGLPNGMSSLFQKSTAISILVATAFFMENLDGTVIATALPQMG
                     KTFHVNPVDLNIGMTAYLLMLAVFIPISGWVADKVGARSVFVSAIFIFTISSVLCGLS
                     NGIMAFTAARIIQGIGGAMMVPVGRLVVLRTTEKNKLMEAIAYITWPALVAPVVGPPV
                     GGYITTYFSWHWIFFLNVPLGIIGIVLSFLWIPNLKEDRVKRFDWIGFVLSATACVTF
                     MYGMELVGREDATWKPTAFYLVCGGVLGIMAVLHFRRATTPLLNLDLLKIKTFAVTLE
                     GGSIFRVAISVSPFLLPLMFQVGFGLNAFRSGMLMLGLFAGNLSMKPATTFMLRRFGF
                     RAVLIWNGILTAIMMLGCAALFPSTARTIMLIVLFVNGLCRSMQFTCLSTLSFADIPK
                     PDLSSATSFFSMITQMSMGMGVAVGAIFLRVAGLLDGNAHGAPTTKEFHIAFFLVAGL
                     TLLGTIDCFSLDREAGALVSGHRLTPLVEKSPATT"
     gene            10696..11409
                     /locus_tag="VG272_01125"
     CDS             10696..11409
                     /locus_tag="VG272_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12309.1"
                     /translation="MRPLPAVFAGWEGGSAAFGTVDAYSDIDLEFIVADDALFDDLYA
                     LAERAIETVSPIAASHTPRSGRYYKLKDGGDFFLVDLIFHRATDPDHQLDIERHGANV
                     PLFDKGNWLRSKPLDERLLAVQRDRRYRELQTWFPMSQVFVRKAILRGQHVEAVSAFW
                     AYTLKPLADLLRMRYCPARWDFGVRYLDRDLPPAVYDQVQDLALVSNLRDLDVKLARA
                     TAWGAALLRELESVGSQER"
     gene            complement(11465..12859)
                     /locus_tag="VG272_01130"
     CDS             complement(11465..12859)
                     /locus_tag="VG272_01130"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HWF12310.1"
                     /translation="MTDFVTQQLIDSTYILAAVGFILALKWLSSPVTAMRGVLIGEIA
                     SGLAVLATLFDPQVLQYKWILIALIIGAGVGVPLGMVKMTAVPQRTAISHALGALSAA
                     LIGIAEYYVAAPHVPKFEMAEIGLEVIIGSLTFTGSLIAAGKLQEILPQRPITYRGQN
                     YVSLSVLGAAIALMVTLVVHPEFTHVFLAMIAVALVFGMMLVTPIGGADMPTVISLLN
                     SYAGFSAALLGFVLNSKVLVVAGALDGASGFILSVNMCKAMNRSFTNVLFGGFGQIHV
                     SAAGTAEQRPVKSATPEEAAAILSAASSVVIVPGYGMAVAQAQHKVRELFDILTKRGI
                     DVKFAIHPVAGRMPGHMNVLLAEADIPYDKMLDMEEINGEFPQTDVALVIGANDVTNP
                     AARTDSSSPIFGMPILDVDKAHTVMVIKRSMAAGFAGIDNPLYYMDKTLMLFGDAKGF
                     VGSIVRELSGGSHG"
     gene            complement(12856..13152)
                     /locus_tag="VG272_01135"
     CDS             complement(12856..13152)
                     /locus_tag="VG272_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HWF12311.1"
                     /translation="MPADFISSLFVFILSAFLGFEVIKRVSPLLHTPLMSLTNALDAI
                     VVVAAIIIAGRHESRLSTILGLVAVAASFCNMVGGFVITDRMLHMFKTGKAKKP"
     gene            complement(13163..14335)
                     /locus_tag="VG272_01140"
     CDS             complement(13163..14335)
                     /locus_tag="VG272_01140"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HWF12312.1"
                     /translation="MIVGVPRETYPGERRVALVPSVIPNLAKAGMEVIVEAGAGAAAG
                     FLDSDYAAKGAKIIVNRAEIFASADIIVQMLCYGSNDETGRADLPLFRRGQVLIGFLR
                     PMGSLETVQEIAASGVTSFSVELMPRTTRAQSMDALSSMGTMCGYKAVLIAADTLPKI
                     FPMLTTAAGTITPARVLVIGAGVAGLQAIATARRLGAVVSAYDLRPASKEQVHSLGAR
                     FVELPIAAKDAQDASGYAKAQDEEFYRRQRELLGKVIAESDVVITAAVVPGKKSPVLV
                     TEEMIKGMAPGSVIVDLAAERGGNCELTKSGEKIMAHGVSIIGYFNFASTVPNDASQM
                     YARNISSFLQLLVKDGKLQVNLEDDIIKSTILTQGGEVINPRVREYFSLPALPAKS"
     gene            14899..15366
                     /locus_tag="VG272_01145"
     CDS             14899..15366
                     /locus_tag="VG272_01145"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HWF12313.1"
                     /translation="MNNAFMQEAIERAVKNVRSGQGGPFGAVVVKSGRVIAAGANSVT
                     TTNDPTAHAEVIAIREACRALGTFQLTGCEIYTSCEPCPMCLGAIYWARPDRVFFAAT
                     ANDAAAAGFDDSFIYEELKLPQEQRKIPLEPLMREAGLEPFREWTNKNDRVNY"
     gene            15429..17666
                     /locus_tag="VG272_01150"
     CDS             15429..17666
                     /locus_tag="VG272_01150"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HWF12314.1"
                     /translation="MEDKYFGHSVIRKEGREKVTGRARYVDDLTVPDMLHGVTVRSNI
                     PRGRIRAIHFGDGIPWNEFTVVTAADIPGKNEVALIEHDQQCLAAEVFNHPEEAIVLL
                     AHPDRYLLEEARRAVHIDVEPLPAILTIEDSIAKRAVIYGEDNVFKSYHMEKGDVDTA
                     WAQADVIVEGEYRTGAQEQLYIETQGMIATANSTDGVTVWGSLQCPYYVHKALVPIFN
                     LPADKIRVIQTETGGGFGGKEEYPSIIAAHAALLALKSGKPVKIIYDRAEDMVATTKR
                     HPSRTRHRTGITRDGKLVAMDIDFVIDGGAYATLSSVVLSRGTIHAAGPYSCPNVRVH
                     SRAMATNAPPHGAFRGFGAPQSIFAIERHMDQLARAVNLSPDEIRRRNFIGKGQTTST
                     GQLIHEDVNFNALLDRAFRESNYHAKRERFSRENPGATIKRGIGFATFMHGAGFTGSG
                     EVYLASVVELEATPEGIVRVLAASTEIGQGTNTIFSQIAAEALGIGYDRIEIAQPDTG
                     AVPNSGPTVASRTSMVVGNLVEKAALDLKQKLVDSGLLCDACTETEFVRACRRYIETR
                     GPLRSQSVYEPPPGLLWDDKKYSGDAYGAYAWAVYVAEVAVDTVTFEARVTDFVALQE
                     VGKVIHPVLAAGQIEGGVAQAIGMALYENVNWRDGRMANGQMTNYIMPTTADIPPIRV
                     FFEEQPYPFGPHGAKGIGELPMDGPAPAIVNAIENAIGIFIREIPATPEVLMEAMTEK
                     ADAIA"
     gene            17653..18162
                     /locus_tag="VG272_01155"
     CDS             17653..18162
                     /locus_tag="VG272_01155"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWF12315.1"
                     /translation="MRLPETVKKIDIDCTVNGERRHVQAFPMARLLDVLREEMNLTGT
                     KEGCGEGECGACAVLVDGAIVNSCLVPAIEANGTDIRTIEGIAHGDQLNAVQEAFLQC
                     GGAQCGICTSGMVIASTALLEVHPHPTDAEIRQGLAGNLCRCTGYQKIFAAVVRACES
                     TANKDAGTR"
     gene            18159..19022
                     /locus_tag="VG272_01160"
     CDS             18159..19022
                     /locus_tag="VG272_01160"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HWF12316.1"
                     /translation="MRAYIPSYDLRVPGSLSEALALLAREPGVWQPFAGGTDLMVLLE
                     AGKLSHKRFFSVARLDDLRGVEMNTDYLTLGALTTYTEVQKHQALQKQFPLLCAAAKE
                     TGSIATQNRGTLGGNIMNASPAADTPPALLVYDAEIELISQRGIRWLPYHGFHTGYKK
                     TRIAPDELLRAIRLPWRTKKWRQYYRKVGTRKAQAISKVCFAGGALLENGAIQDVRIA
                     LGSVAPVVQRAVKTEDALRGNRITPTMIATAKEILLREIAPIDDIRSTARYRLRVAQN
                     LLEEFLLQLDK"
     gene            19019..20380
                     /gene="allB"
                     /locus_tag="VG272_01165"
     CDS             19019..20380
                     /gene="allB"
                     /locus_tag="VG272_01165"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR03178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HWF12317.1"
                     /translation="MTTTRAIRSKRVVTPEGVRPATVLLRGGLIEAISGYTDSSAGKN
                     VLDAGEFVVMPGLVDTHVHINEPGRTDWEGFESATRAAAAGGVTTLIDMPLNSIPATT
                     TLAALRTKRKAARNKCWVNVGFWGGVVPGNTADLSALHRAGAFGFKCFLVPSGVPEFA
                     NVNETDLRAALPVLAELNAPLLVHAELPGPIDKAGEKLANSDPAKYENWLLSRPAVAE
                     TKAIEMIIRLARECRARVHIVHVSSELSLPLIRRAKTQGVRITAETCPHYLFFDSWSI
                     GNGRTEYKCAPPIRDAKNNKKLLLALSHGEINFVVSDHSPSPPAMKCLDSGNFFKAWG
                     GIASLQLGLPAMWTTMRAQKYSLEHLAHWMCAAPAQLAGLDKSKGSLAKGYDADIVIW
                     NPDKSFTVQPKMLQHRHKLTPYLKHQLHGVVETTFLRGEMIYDRGRFLGKPKGDLLRR
                     GGR"
     gene            20380..21387
                     /gene="alc"
                     /locus_tag="VG272_01170"
     CDS             20380..21387
                     /gene="alc"
                     /locus_tag="VG272_01170"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HWF12318.1"
                     /translation="MDFTEYIDLAAERLGGRVLAANDEFFAPKENLLKASAPVWIADK
                     YTDVGKWMDGWETRRRRTPGYDWCIVQLGLPGVIHGVVVDTSYFKGNYPEHCEIEACA
                     VDGDPSLDQLEDSATQWRNLLPKSPLTGDSRNLFPLAPLERVTHLRLKIYPDGGVARL
                     RVHGEVVPDWPSLTRAGGEIDLAAVEHGGWVIVSSDMFFGSRNNLIQPGRSTGMHDGW
                     ETRRRRGPGHDWCILRLGTAGKISRVEVDTSHFKGNFPESCSLEVCAAPRDLVDPDKL
                     ETLPWKEILPRTKLKASSLHIYENELQQMGGATHARFHIYPDGGVARLRLFGKPATRS
                     A"
     gene            21389..21904
                     /gene="uraD"
                     /locus_tag="VG272_01175"
     CDS             21389..21904
                     /gene="uraD"
                     /locus_tag="VG272_01175"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:TIGR03180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HWF12319.1"
                     /translation="MTLADFNALPSNQAVSLLMDCCGSTRWVATIAARRPYADPEELH
                     KTADSVWWKLDRADWLEAFSHHPQIGDKPASGSESARQWAEGEQNGARIASDDVKTRL
                     ARANRAYFEKFGYIYIVCATGKTAEGMLAILNQRLQNDQASELSIAAEQQRLITRIRL
                     QKLLAGEDRSS"
     gene            21901..22239
                     /gene="uraH"
                     /locus_tag="VG272_01180"
     CDS             21901..22239
                     /gene="uraH"
                     /locus_tag="VG272_01180"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR02962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HWF12320.1"
                     /translation="MSAISTHVLDTARGCPGAGIRVQLHRLSGEAWKLIGESHTDENG
                     RCGNLTGNEKPEPGTYRLIFHAAEYFENQNTKTFYSEIPVIFEVGDSGRHYHVPLLIS
                     PFGYSTYRGS"
     gene            complement(22322..23764)
                     /locus_tag="VG272_01185"
     CDS             complement(22322..23764)
                     /locus_tag="VG272_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12321.1"
                     /translation="MKTSLSDSAIRSALEILAGPSAAFARRYPGETGRRQPVHVVYGG
                     AHLFHSNISRRLGEGALAALAEFAPDAFSFARAIGLPGAATLSKTGKVAVAAERQFAK
                     APEKLRLTNRPAWLAQKIYTLVLEKLRREPVEDFRIDFEDGYGNRPDEEEDQHAKLTA
                     QQVAKGFADNSLPPFIGIRLKPFSPELRDRSIRTLNIFLSTLCEATGGRLPANFVVTL
                     PKVVIPAQMAALSQLLAAMEPELGLEKGALRFEMMIETTSAILNARGESSLPQFLDAA
                     GGRCIAAHFGTYDYTASCSITAAYQRMDHPACDFAKNMMQVAFAGTGIWLSDGATNVM
                     PTPLHRAEKGKRLTRAQQKENLEAVHRAWKLHYGHIQHSLVTAYYQGWDLHPAQLPTR
                     YASVFSFFLEGLDSSAARLKNFVEKAAKATLVGDVFDDAATGQGLLNFFLRAINCGAI
                     SEAEAERLTGVTIEELHFGSFLKILKNRTK"
     gene            complement(24521..24718)
                     /gene="rpsU"
                     /locus_tag="VG272_01190"
     CDS             complement(24521..24718)
                     /gene="rpsU"
                     /locus_tag="VG272_01190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HWF12322.1"
                     /translation="MAEVSIQEGESLENALRRFKRKVQQEDIIKEIKKHSYYMKPGEK
                     RRAKQALSRKRLRKKQRRELD"
     gene            24988..26220
                     /gene="glgC"
                     /locus_tag="VG272_01195"
     CDS             24988..26220
                     /gene="glgC"
                     /locus_tag="VG272_01195"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HWF12323.1"
                     /translation="MSLMRDAIGVLLAGGQGERLWPLTRDRAKPAVPFGGVYRIVDIT
                     LSNCINSDLRRVFVLTQYKALSLNRHTRQGWSQLLGLGEFIEVLPPQMRVSTNWYLGT
                     ADAVYQNIYSIGSEHSRYVIVLSGDHIYKMNYQKMLQQHVDSGADVTVATLEVPSTEA
                     AGRFGVLETDANWRIVGFEEKPAAPKRSHLHPDMVNASMGVYVFNTQLLIPALLADAE
                     DPNSKHDFGKDILPRIFSKYRVFAYNFIDENKIGPQYWRDVGTIEAYYEANMDLVSVS
                     PIFNLYDKSWPIRTWQHQYPPVKTVFADPGRMGVSMDSMVGGGSIISGGRVTRSVLGY
                     DVRTNSYSEVENSILFNHVNVGRHARIRNAIIDRHVTIPERAEIGYDMEADRQRYHVT
                     EGGIVVVVREESMIEEPE"
     gene            26400..27704
                     /gene="eno"
                     /locus_tag="VG272_01200"
     CDS             26400..27704
                     /gene="eno"
                     /locus_tag="VG272_01200"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HWF12324.1"
                     /translation="MSTKIARVHGRQIIDSRGNPTVEADVILEGGAVGRAAVPSGAST
                     GEHEAIELRDGDKSRYLGKGVLKAVGNVNGAIAKAVAGLDASDQKALDRRMIETDGSP
                     NKGNLGANAILAVSMAAARAAAVAARQPLYKYLSRYSSDPSGSTLPVPMMNILNGGAH
                     ADNSVDFQEFMAMPVGAKTFDEALRMGVEVFHRLKTVLTKRGYSTAVGDEGGFAPNLK
                     SNEEAIEVILEAIAAAGYKPGEHVAIALDPASSEFYEKASGNYVFKKSDKSAHTSEQM
                     TRFYADWVRQFPIVSIEDGLAEDDWAGWKHMTAELGSKTQLVGDDLFVTNTVRLQRGI
                     SEHIGNAILIKLNQIGTVTETIEAIEMARKAGYASIVSHRSGETEDTFIADLVVAMGT
                     GQIKTGSASRTDRVAKYNQLLRIEEELGSAAKFPGKAALVRK"
     gene            27726..29285
                     /gene="gpmI"
                     /locus_tag="VG272_01205"
     CDS             27726..29285
                     /gene="gpmI"
                     /locus_tag="VG272_01205"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HWF12325.1"
                     /translation="MPKRPKPIVLTVLDGWGYRVETKGNAIALARKPNYDRLLKDFPN
                     TLIQTSGPFVGLPEGQMGNSEVGHLNMGAGRIIHMDITRIDVMIVTGEFFKQPLLLEA
                     MERGRARNLHLLGLLSDGGVHSHMQHLYALLRMAREHHVERVFIHCFMDGRDTHPHSG
                     VEYIQQLQQKIREIGVGQIASLTGRYFAMDRDNRWDRVEKAYRAVVHGDAPTKSADPL
                     EAIRRSYERGVTDEFVEPIAIASGTSPSAPTVGPVRDDDAVIFFNFRADRARQMTRAI
                     AEPGFKEFSDIRRPTNLFYVAMTQYEKTWPWLRYVIAPEKIAHILAQVFAERNLKNLR
                     CAETEKYAHVTYFFNGGIEKPYGGEERILVPSPKVPTYDLKPEMSAVGIAETVVKAVE
                     KDAFDAVVMNFANADMVGHTGSLEAAIKAVEAVDGCLGIIRQALQPRGGAWIITADHG
                     NAETMIDPVTGGPHTYHTTNPVPLILATENEIGPLAPGGSLRDVAPTLLGILGFPVPP
                     EMTGHDLRLKK"
     gene            complement(29452..30483)
                     /locus_tag="VG272_01210"
     CDS             complement(29452..30483)
                     /locus_tag="VG272_01210"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF12326.1"
                     /translation="MVNLQEVLDPLDILRKLTGIRIQDMYNKMLRHGWTLGSPQLMRV
                     LQLVVRSYHGPSVRVLEEFWKKRSPDMVVSLVPHFNRAMREAYEKACPGRPFVTVLTD
                     LADYPKHFWIEKESQYIVCGTEHAVEQARQLGHLDGTVFRTSGMILHPRFYEPVEVDR
                     SAERKKLGLNPDLPTGIVLFGGYGTWKMLDIVRRINRSSLQVQLILICGRNEKLAEAL
                     RQEPSRIPLHIEGFTTQIPYFMALSDFFIGKPGPGSISEALSKHLPVIIDCNAWTLPQ
                     ERYNAQWVREKEVGMVVDSHRETAKALAKLLEPGALAQYQARAAAVNNRAIFEIPEIF
                     EKILERGAQ"
     gene            30745..31626
                     /locus_tag="VG272_01215"
     CDS             30745..31626
                     /locus_tag="VG272_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HWF12327.1"
                     /translation="MRCDLHVHSLHSGTLPVALLRHFFLESYSAPTDVYSVLRKRGMD
                     LITLTDHDSITGAEELRRHPDFFVSEEVTCTMPSGTQAHVGVYDITERQHTEINRRRN
                     EMVRLLAYLSEQRLFFTINHIFSSVTGKRHTDDYEWFSGYFPAVEARNGQMLPAANKL
                     AADFAWKRCKTQVAGSDAHALASVGTTYTEVPGAKNKKEFFAGLRVGHGKLCGESGGY
                     WKLTRDILLLCGEMMRKDPWTVLLAPLVPLVPAGVAVDYFVDLSFIRRWGTAINGPQP
                     APKDLLETLPLRQKQMA"
     gene            31691..32227
                     /locus_tag="VG272_01220"
     CDS             31691..32227
                     /locus_tag="VG272_01220"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWF12328.1"
                     /translation="MTARRVVWEFIQARDYQVMRKVHRWRAPRWLRYWMIASTKLGDG
                     WIWYSVGIALLLFGGDMRFIAFAASASAEAATVVIFRYIKKVSKRKRPCHMESHCWAN
                     ILPPDQFSFPSGHAMSAFAIATPLCIFYPELQATLIALSVSIALSRVILGMHFVSDVV
                     VGSLVGFGLGYGAYLLVR"
     gene            complement(32343..33542)
                     /locus_tag="VG272_01225"
     CDS             complement(32343..33542)
                     /locus_tag="VG272_01225"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HWF12329.1"
                     /translation="MIDAVIVSSVRTPVGKAFKGTLRSTRPDDLAAIAMQEAIRRVPG
                     LNPADIEDVILGCAMPEGEAGMNIARIASQRAGLPPEVPAITINRFCSSGLQAIAMAA
                     ERIMVGHAGVILAGGTESMSMIPMGGNKVSPNPWLMDHYPDAYLSMGLTAENVAKKYG
                     ITREKADEFSLASHMKALAAIAAGKFNEEIVPVEVQTTVAATTANGRGAKTKTTTTLF
                     DMDEGPRADTSLEALAKLKPAFHVRGTTTAGNSSQTSDGAAATIVMSAERARALGAKP
                     LARFLGFATAGVPAEIMGIGPAFAIPKVLKLTGLTLDQMDVIELNEAFAVQSLAVIQE
                     AGLDPARINPNGGAIALGHPLGCTGAKLTASILRELDRRNARYGMVTMCIGGGMGAAG
                     IFERIAS"
     gene            complement(33764..34375)
                     /locus_tag="VG272_01230"
     CDS             complement(33764..34375)
                     /locus_tag="VG272_01230"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HWF12330.1"
                     /translation="MRLAIRIIARALYRAGGLGILALGVFDSSPLVLPFGNDLLILAL
                     SARHHDRMIYYALMATLGSVIGCLATVWLSRKGGERLKESSSGMRLGRVQDLVQKNAA
                     WTLLSASLLPPPFPFTAVVAAAAAFKYPRKKLFSFVGVGRFARFLIEGALAVHYGRWI
                     VRQEKSPVLEDAMIVLIVIAIAASIFSVYQWSGKNKHPAGQVA"
     gene            complement(34597..>34861)
                     /locus_tag="VG272_01235"
     CDS             complement(34597..>34861)
                     /locus_tag="VG272_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWF12331.1"
                     /translation="ILDNLSSHKVAGVKEAITAAQATLLYLPPYSPDLNPIEKFFAKL
                     KARLRQAATRTVDSLWEKIGQVLNTVSSTECTHYFASSGYVCT"
BASE COUNT         8098 a         9457 c         9494 g         7812 t
ORIGIN      
        1 aataacctgg cggccactta ccacgatcaa tccatgaacc tccaggccga gcaatcctac
       61 aaacgggcca tgtcgattct ggaaaagagc ccgaatcaga atgtttccat gggacgtaca
      121 ttgaacaatc ttgcatcgct atatcaagat atgggcgcgg atgcaaaagc cgaaccgctg
      181 tacaaccggg cgatgggcat ctggaagaat ttggcgaaga aagataatgc cggcgaagcc
      241 tttacggcaa tcagcctggc tggaatttat cacacccgga atcaggatga agttgccgag
      301 cctctttacc tgcgagctct gacactgtgg aatgcggaag gcaaggcgga ctccgaaatt
      361 gctgccgacg cgttcattca tttggccgga atctaccgct tccgaagcga taacgaaagt
      421 gcagcgtcca tgtatgcccg cgtaatcgcg atctgggaaa agaccggcga catcgacaaa
      481 tcagaaggca tcgcggcaca agcggctctt ggagaaatct accgctctga aggaaagtac
      541 gagcaagccg aacccctgct cgaaggcgcg ctgaagggaa ccgaaaaaat ggtcgggtcc
      601 gatgatcttc aagtcgctgc cagcctgaac aatctcgcgg ttctcttcta ttgcgaggga
      661 aattatcagg cagctgaacc tctctacaag cgagtgttgg atatccgcca gaagaatctc
      721 ggcgctgaca acccgatggc gctgcagaca gtggaaatct atgccacact gcttcgccat
      781 gaaggcaaac aggtcgacgc ggctaagctc gaagcgcagg ctgcacgtgc aggagttgcc
      841 cacctttaaa tacccggccg cggcccgcct ctcgcagcgg gccgcggtca cctcctcact
      901 acacccgcat cacacttcaa tcctccctga gaaaacacaa agaacttaaa aatgccacgc
      961 aaacaagcgc acgatctttt ttcgtttgaa atcttggttg aaacgcgtta atcgcaccat
     1021 gcgctgtgag gccctggtag tagtataggt tttcgtgatg tgccatatcc cccagttcaa
     1081 ataatttggt tctgcaaaag ttgactcggg tgtataagtg tgcccattcg acagagatta
     1141 ttcgaaaaca gttatttgtt cttaagctga aaactttaag agtgaagtac tcgcacgatg
     1201 cgaaaatttt gcatgcttgt gggattgatg atcctggccg ctggaactgc atcggcgcag
     1261 gcggtggacg cgaaaagcgt tggaagtctc tgccaaggcc atgggcgcga ctaacttaaa
     1321 aacgattcaa tactcgggtg acggctggtc ttcgatgatt ggccaaacgt atgggcttgc
     1381 agaagattgg ccgcactacg aagtgagtgg ctatacgcgt gtgattgact acgacgcaaa
     1441 gtggtcgcgc gaggattaca cgcgcaggca gggaaagtac ccgcttcttg gcagagtgcc
     1501 aatgccggag cagcacatga cttccatttt gagtggaacc tacgcatggg acatgcaagg
     1561 cgatgttccg gtgccgctga cgcgattgta tctggacggc gtaacgtatt cggatttgcg
     1621 gcagcttgag attgtgctga ctccgcatgg ttttctgaag gctgcgctcg cggcaaaaga
     1681 tgcgacagcg atttcactgc cgattatcgg cgcgtcggat tttggtcttt ctcaatttgg
     1741 caggaaggtg acagtcgtgt ccttcaccat gggaaagtac aaagtgaatg gaaccattaa
     1801 cgataaaaat ctcgttgagc tagtggacac ctggtatccg aatcctgttt acggcgacat
     1861 ggattacgaa atgcgctaca cgaaataccg ggatttcggc ggcatcatgt ttccgggaca
     1921 agttcacgtg catcagggcg atccgcgttt gaatccggcg cacaactact atgaattcaa
     1981 cattacggac gtgaaggcca atgtgcccgt cactcccatg cccgttccgg aatcggtgcg
     2041 aaccgcaacg cttgcctcag ttaaggttga aagtcaaaaa ttggcggacg gagtgtggct
     2101 catcggcggc gggacgcata acagtgtgct tgtcgaattc aaggatttcg ttgccgtagt
     2161 cgaagctccg caaaatgaag cacgctctct agccgtgatc gctgaagtga ataagctggt
     2221 cccaaacaag ccgatccgtt acctcgttaa cacgcatcat catatggatc acgccggcgg
     2281 cctgcgtacc tatctttctc aaggcaccac gattgtgact cacgaatcga acaagcaata
     2341 ttatctcgac attatgttct atccggctcc acgggaactt gatcctgacc gcatggcgat
     2401 gttcagtccg atgtatatga ttagcaggcg cccggctccg attgatactg tgggcggcga
     2461 tacgcgaggc gaaggaaaat acgtggtgac cgacggcgag cacgtgatgg agatatttca
     2521 tgtgcaggac atgggctacg aactaggcga tccttcctac aaccagggaa atcacagtgc
     2581 cgacatgtta atggcgtatc tgccgaaaga gaaaattctg attaatgctg atctttattc
     2641 accagcggaa cctggtgcgc agccacccgc agcaacggcg gcgatgaaaa ctttgtatca
     2701 gaacatgctg aagctgaaac tcgatgttgt gcagcatgtt ccgatccacg gacatgccgg
     2761 gacgaacgct gattttttga agatcttcgg caaagggcag taggcattta gtatcgcggg
     2821 cttcaacctg cgggggttcg tccttgcaag gacgaaattc cgcaggctga agcctgcgcc
     2881 acataaacac atgaaaatat ttctcgacga gagtttgaca cgaattgatg ccgtgaatat
     2941 agtctgcgta caagttacgc acagattgtc agatcgacag gacaatcgcg aagtttggaa
     3001 tttcggggag tgaaaaacca aaatgagtga atccgccaaa attgttgaag ttaaatctcc
     3061 cgagttgctc aacttgatcg acaaaaatgc agtcgtcgaa caaatttgca cgggcttttg
     3121 gtttacagaa ggccccatct ggaatgcgaa agaacagtgc ctgtatttca gcgacatgcc
     3181 gggcgatgtg cgccgccgct ggagcgccac ggacggcgta aaggaagtcc gcaagcccag
     3241 caacaagtgc aacggcatga catacgacgg cgtgggcaat ttgtatgttt gcgagcacgt
     3301 gacgagttcg ctggtgatgg aaactccctc gggcgagcgc aaagtgcttg cctcacactg
     3361 gcagggcaaa gaactgaaca gccccaatga tgttgtggtt cgctccgata attccgttta
     3421 ttttagcgat ccctcctatg gccgaatgcc ggtctttggc ctggaacgta aacaagagct
     3481 cgattttcag ggcctttaca ggatttctcc tgacggaaaa ctgcatctgg aagcaaacga
     3541 ttttggccag acaaatggcc tgtgtttttc gcccaatgaa agaattctgt acgtgaacga
     3601 ttctcctcgc gctcacattc gaatgttcga tgtggctgcc gacggctcgc tgagcaacag
     3661 ccgaattttc gctgaacaaa tcggagatgg tattctggag aacggaattg tcgatggcat
     3721 gaaggtcgat gagcggggga atatttttgt tacgggcccg cgcggaattt gggttttcag
     3781 tccaacggcg gaacatctcg gcgtcattcg aatgccggaa catgcaggaa atcttacgtt
     3841 tggcgggcga aactgggatg agctgtactg tgcgtgttcg aaatcgattt atcgcttaaa
     3901 aatgaaagtt cgtggaaatc ctgtcgcgta catgaagatg cgctaagagg aaaaaatggc
     3961 cgagcgaaaa ctgaatgcaa aaaaaacggc gctgatcatt caagatttgc aaaatgacgt
     4021 gatcaccgac ggcggcgcct tcgcttcctc cggcgcgccc gcgcatgcca agaaacagaa
     4081 cgtggtggag aatgtaaaaa ctttggctgc cgccatgcgc aaggccggcg cgatcgtgat
     4141 tcacgtgcac tacatcgtcg aacctggcgc gccgggatta aaattgaacg cgccgctctt
     4201 ccgcggattg gccgaaagta aggccgtcgt gcgcgggact tggggagcgg ctcctgccga
     4261 agggctcgag ccgcaaaagg gcgacatcct cgttgaaaag atgcgtatga atggttttca
     4321 cgggacgaag ctcgaaacaa ttctgcaggg ttacgggatc gattcgctcg tgataaccgg
     4381 cgcctggacc aacttttcca tcgagcatac ggcgcggcac ggcgcggatg ccggctatga
     4441 cgtcgtcgtc gcgaccgacg gaacatccac agtgagcgac gagtggcaaa acgcaggact
     4501 taattatgcc cttcagaata tcgccgaccg actgagctgc caggaaatca ttcaggcttt
     4561 gagttaatct aaatagggtt cgaaccattc cgatcatcat agagagtgca tgactattcc
     4621 tctggaaaca tccatcgtcg tttctgcaga agacgtacgc gcaaccatcg ttcacgtcct
     4681 ttccagactc ggggccaatg agcgtgaatc ttccattcaa gcggatgttc tgacggaagc
     4741 ggaccttcgc ggccatcatt cgcatggttt acagcgcctg cccgttctcg cagcgcgaat
     4801 caaaaaacag ctgatccgag taaaggccga gcctgattac atctggacag cggattcggt
     4861 gctgagtgtc gatggaaaag atgggctggg accgtttgtc gctgagaccg cgcacgagga
     4921 agcgaaatcc cggctacagc aacaagggat cgtcgccgtg gcgattcgaa acagcagcca
     4981 cattggaatg attgggtact attgcgagaa gcgcgcacac gaaggttttg tttgcatcgc
     5041 gatgacggaa agcgaagctc tcgttcatcc acacggcggc acaaaagcac ttgtcggcac
     5101 aaatccaatc gctattggta ttccgagtga gccttcgcca tttgttttcg atatggccac
     5161 gtctacgagc gccattggaa agatttacgc cagcaaacat cgcggcgaaa acattccgct
     5221 cggctgggcc atcgatgcgg aaggcaatcc aactacaaat gcagaggcag cgctaaaagg
     5281 ctcgctgaca cctctggccg ggccgaaggg ttacggattg ggaatttcca tcggaatact
     5341 tgccggactt ttgccaggcg cggaaattgg acgcgccgtg cttggtacgc tggatacgga
     5401 gtttcgctgc accaagggag atttatttat tttgatgaat cctgcggcgt ttccgggcgg
     5461 ccctatgctt gctgcacatg tggcatcgta tctcgacgaa ctccgcaact ctccgccaca
     5521 gaaaaattgc gaacgagtca ttgtgcccgg cgatcgtgcg aggaaaatcc gcgaagaacg
     5581 gatgtgctcc ggaataccgc taccaaaaga agtttggctc gcagcagaaa ggctgaaaaa
     5641 tgagttctga aatgagtaat cacgttctgg gattttcaac cgcaggcaga attttctttg
     5701 gctggggcgc ggcgagcgtc ttgcgtgatg tcgcgaaaga attcggccct cgagtattta
     5761 tttgcaccga ccggaacatg gtcaaagcgg gtgtgtgcga aaaagtgttt gcgaatcttg
     5821 ccgagggcaa ggccgaagtt aaaattttcg atggcgggca acctgaagtc gatcgccaga
     5881 cgattgaaac cgccgcagca gcggcgcagg cgtatgcgcc cgacgtggtg cttggcctgg
     5941 gcggtggcag taatcttgat ctttccaaag ctgtcgcgct gctagtgaga tatcccggat
     6001 cgctttcttc ctactatggc gaaaataatg tccctgggcc gattgcgccg gtcgttgcag
     6061 ttccaacgac tgcgggcact ggatccgaag taagcccggt cgccgttgtg gctgatgcgg
     6121 aacgcgcgat gaaaattggc atcgcatcgc gcgcgctgat tccgaagtgg gcgattgtcg
     6181 atccagcatt gaccgtttcg tgtcctgcga gcgttaccgc tcattctgga atggatgcgc
     6241 tcgtccacgc aattgaatct tactgcgcac gtgttcggac cgattgctct cctcacgcca
     6301 ttttcgtcgg caaaaatcct gtgtcagatg ttctggcaac gaaggcgatc actgcgatta
     6361 ctaaatttct tccgatcgcc gtcgggcagc caaataaccg tgaagcacga gagcaaatgg
     6421 ctctcggaag cctgctcgct ggaatggcat tttccgctgc tggaacggcc gctgtacacg
     6481 cgctgcaata tcctgttgga gaagcaacgc acactccgca cggtctggga aacgctgtgc
     6541 tactgtccac cgtgatgaaa cgcattacgt cttcgcgtgt cgaagaaatg gcattcattg
     6601 cgcgcagtct ggattcgagc ctccactcac aaactaacgc ggaggcagcg gcgaaggctg
     6661 caatactggt cgatgagttc gcctcaaaag tgggaatccc aaaaggcctg cacacgttgg
     6721 gcgtgaagga agcgcagctc cctgagcttg caaaaatggc aactacagtg aaaaggcttt
     6781 tggacaatag ccccgtttcg tttgatgaaa gcgatcttct gcatcttctt caggacgctt
     6841 tttagaaatt cactttatac attcatggca cagtgcttcc ccgtccttac aaggatttgc
     6901 gaggtttact gcaaagaggc cccggtgtat aagtttcctc accgagatgg tcagcctgga
     6961 aaatccgact gagtaacgag ggggaaaatc aatgagcaag aaaaatggca tcttggcatt
     7021 cggcttggca gcaggcctgt gcctgctggt tttatttagc gcgcgaagcg cgcgaacgca
     7081 gaacgggccg aaatataaag tggatgcgga atggccaaaa ccgctgccga ataaatggaa
     7141 gatgggtggc gttacaggac tcgccgtaga caaagacgac aacgtttggg tgcatgatcg
     7201 tccgaatgat ttgacggaca ttgaactgga agcggaactc aatcccccga tcgccgattg
     7261 ttgtgtgcgg cctccgtcga tgattcacat cgataaggaa ggcaacgtca tcgggtcaat
     7321 cgatccgccg cagggtcacg gaatggccgt cgacagcaaa ggttttattt atcttggcaa
     7381 cgtggtaaac ggaatcggca atgtacgcaa atacgatccg aaaaccggag cgttcatttc
     7441 tgaattgccg cacactcccg acatgccgcc cggaggcgga ggcggcgacg ctgctccgga
     7501 taatccgcgc gtcccgggcc acggcagcat gggccctgtt tttggatttc tgcccggcgg
     7561 acgtccgaaa ccaaatcccg ctgcgcgagc ggcccgcgaa gcagcgaccg tcgcgtttcg
     7621 cgcaaaatat cctccgacaa cgcccatgat cgtgggcggc attgaagaag tgcggctcga
     7681 cgaaccggcg cacgaacttt acgcggctga taactatctg ggcgggcgtg tgatggtgtt
     7741 tgatatggat accttccaat tcaagcgcgg ttggggcgcc tacggccata aactatcgga
     7801 aatcacgatc gatgataaag accgcgctta cgtccccaac gggccaatgc ccaaggaatt
     7861 ccgcggccac cttacattga atgtctctca cgatgggctc gtgtatgccg ccgaccgcat
     7921 ggcgaatcgc atcgacgtga ccaccaagga cgggaaattc ttgaaggaat tcatcatggc
     7981 accgtggaca ggcgtcggcg gctcgacggg cggcgtgatg ttctcgccgg acaaagagca
     8041 aagattcctt tacatttccg atctcacaaa caatcacatt tggtttctga atcgcgagga
     8101 cggcaaagtc gtgagcaccc ttggaagcat ggggcaaaat gcgggccagt tttacggact
     8161 gcacatgatt tccgtggact ccaaagggaa catttatacg ggcgaagttt ttggcggaga
     8221 gcgcgttcag cggtttgtcc cggtcaagta gtatctgatt tgaactgaaa gtgtgattcg
     8281 attgctgttt cgctccgatt tggtgcgccc tgcatggcgg tttacaattg ccggccatgc
     8341 agggctaact gcttgcggtg tacgtaatgc ctccagccga taaatatcgc ttagtaatca
     8401 atagctttcc ggtatctgaa aagtatactt tcgcaatgtt taatcaatcg atttgagatc
     8461 atggataata tttgcgggcg tcgccgaaaa aacggtgctc acaaaaatca atgaaagcaa
     8521 ccagtctgag aatatggcta aaaaagacag acgaatgatt cagcgatttc aggccaagcc
     8581 ggacgatttc attctctatg acgaaaggtc cgcccctgtt cgagatgtaa gcatggaagg
     8641 cgttttcgtt ttggatcccg atccgcttcc ggttggctct gaaatcgtgt tcatatttcg
     8701 cgcaggacat caggacattt cgctcgaggg aattgtccgc cattcggtgg accagcaagg
     8761 catgggcatc cacttcacca agattccggc cgtatcgaaa cggcgtttga tcatccacat
     8821 cgctactttg gcgcccgctc ccttcgcact ggacatgccc tcttaaagac agcgatttca
     8881 catgccgctt cattcactgc aaggcacgct ccctttacaa aaaatgggta gacacgtaga
     8941 gtttacgggc ggctacttga aggttcgcgc gtttacagcg ccggcgaatc ttgcgcgggt
     9001 tacctaacgg aatgtcatct ctttttcaaa aatccacagc gatctccatc ctggtcgcca
     9061 ccgcattctt catggaaaat ttggacggca ccgtaattgc cactgccctg ccgcaaatgg
     9121 gcaaaacatt ccacgtaaat cctgtcgatc tgaacatagg aatgactgcc tatttgctca
     9181 tgcttgcggt ttttattccg attagcggat gggttgcgga taaagtgggc gcacgttcgg
     9241 tttttgtttc ggccattttc attttcacaa tttcttccgt actttgcggg ctgagcaatg
     9301 gaatcatggc gtttaccgcc gcgcgcatta ttcagggaat tggcggcgcc atgatggttc
     9361 ccgtcggtcg gctagttgtg ttacgcacga cggaaaaaaa caagttgatg gaagcgattg
     9421 cctacattac gtggcctgcg ctggttgcgc ccgttgtggg gcctcccgtc ggcggttaca
     9481 tcaccactta tttttcgtgg cactggattt tctttctgaa tgtgccgctg gggattattg
     9541 gaattgtgct ctcttttctg tggattccga atttgaaaga agacagggta aagcgtttcg
     9601 attggattgg gtttgtgttg agcgccacag cttgcgtgac gttcatgtat ggaatggaac
     9661 tcgtcggccg agaagatgcg acttggaaac ccactgcgtt ttacctggtt tgtggaggtg
     9721 ttcttggaat catggctgta ttgcatttcc ggcgcgcgac cactcccctg ctgaatctcg
     9781 atctgctcaa gatcaaaaca tttgcggtca cgttagaggg gggatcgatc ttccgtgttg
     9841 caatcagcgt atcgcctttt ctgcttccgc tgatgtttca agtgggattc ggtttaaatg
     9901 cgtttcgatc gggaatgctg atgctgggtc tgttcgctgg gaatttgagc atgaagcctg
     9961 ctacgacatt tatgctgcgg cgctttggtt tccgcgcggt gctcatctgg aatggaattt
    10021 taacggcgat tatgatgctg ggttgcgccg ccctgtttcc ctccacggcg cgaacgatca
    10081 tgttgattgt gctcttcgtg aatggtcttt gccgctccat gcagttcact tgcttatcga
    10141 cactttcttt cgcggatatc ccgaagccgg acctcagctc agcgaccagc tttttcagca
    10201 tgatcacgca gatgtcgatg ggaatgggcg tggcggttgg agcaattttt ctgcgcgttg
    10261 caggattgct ggatggcaac gcgcatggag ctcccaccac taaggaattt cacatcgcgt
    10321 tttttcttgt cgcgggattg acgctactcg gaacgatcga ttgtttctct cttgaccggg
    10381 aagcaggagc gctggtcagc gggcatcgat tgacgcctct tgttgagaaa agtcctgcca
    10441 caacgtaaat taagaaccgc acccttgcaa aaaaagcaag agtgcgacac ccaaatcgag
    10501 acgaactcac acaaagcgat ttgaaattat ggtagtgggt cagtttgaaa tcgactgcag
    10561 gccctacttc acggccgttg aggacgcctt taccgggaac gtggactacg cgatgctggt
    10621 cattaggagc tgcctttcgc catgatgatt aaccagtctc tccgggatcg ggtctccgat
    10681 gccgttaccc aggccttgag accgctaccg gccgtgtttg ccgggtggga gggtggcagc
    10741 gcggctttcg gtaccgtgga tgcctactcg gacatcgact tggagttcat agtggccgat
    10801 gatgcattat tcgatgacct gtacgcgtta gcagagagag ccatcgagac ggtgtctcct
    10861 attgctgcca gccacacccc gcggagtggt cgatactaca agctaaaaga tggtggcgac
    10921 ttcttcctgg tcgacctgat ctttcaccgg gccacagacc ccgatcatca gctcgacatc
    10981 gagcgacatg gtgcgaacgt gcctctcttc gacaagggaa attggctgcg atccaagccc
    11041 ctcgacgagc gattgttggc ggtgcagcgg gaccggcgct atcgggaact tcagacgtgg
    11101 tttcccatga gccaggtctt tgtccgcaaa gccattctgc gcggccagca cgtcgaggcc
    11161 gttagcgcgt tctgggcgta tacgttgaag ccgctagccg atctcctgcg catgcgttac
    11221 tgtccagcgc gatgggattt cggtgtgcgc tacctcgatc gcgatctgcc tcctgctgtg
    11281 tacgatcagg ttcaggacct tgcgctcgtc agtaaccttc gagacctcga cgtcaagttg
    11341 gcgagagcta cggcctgggg tgccgcgctg ttgcgagaat tggaatcggt gggcagccag
    11401 gagcgttagg tgctccggca gatcaatcgc gccccgtcag gcccaaactg acccactacc
    11461 gaaattatcc gtggctgccg ccggaaagtt cgcggacgat tgatccgacg aatcccttgg
    11521 catcgccgaa gagcatcaga gtcttgtcca tgtagtagag cgggttgtcg attccggcga
    11581 agcctgcggc catgctgcgc ttgatgacca tcacggtgtg cgccttgtcg acgtccagaa
    11641 tcggcatgcc gaagatggga ctcgacgaat cagtgcgtgc cgcgggattc gtgacatcat
    11701 tcgctccgat gacgagagcc acatcagtct gcggaaactc accgttgatc tcttccatgt
    11761 cgagcatctt gtcgtagggg atgtctgctt cggcgagcag cacgttcata tggcctggca
    11821 tgcgccccgc tacggggtgt atcgcgaact tcacgtcgat gccgcgcttg gtgagaatgt
    11881 caaagagttc gcggactttg tgctgcgcct gcgccacggc catgccgtag ccgggtacaa
    11941 tcactacact gctcgccgcg gaaagaattg ctgccgcctc ttcgggtgtg gcgcttttca
    12001 cagggcgctg ttctgccgtg cctgcggcgc taacatgaat ttgcccgaaa ccaccaaaga
    12061 gcacattggt gaacgagcgg ttcatcgcct tgcacatatt caccgaaaga atgaagccgg
    12121 aagctccgtc cagcgcaccc gccactacca gcactttgct gttgagcacg aagcctagca
    12181 gcgccgcgga aaacccggcg taggagttca acagtgagat cactgtaggc atgtccgcac
    12241 cgccaatcgg ggtgaccagc atcatgccaa acaccagcgc aaccgcgatc atggcgagaa
    12301 agacgtgcgt aaattccggg tgcacgacga gcgtcaccat cagcgcgatt gccgcgccaa
    12361 gtacagacaa gctgacgtag ttctgtcctc gatacgtgat gggccgctgc ggcagaattt
    12421 cctggagttt gcctgcggcg atcaaactcc ccgtaaaggt gagcgaaccg ataatgacct
    12481 cgagcccgat ttccgccatc tcgaacttag gcacgtgggg cgcagccaca taatattccg
    12541 cgatgccaat cagtgccgcc gaaagagccc ccaacgcgtg gctgatcgcg gttcgctgcg
    12601 gcacagccgt catcttcacc atgccaagag gaacaccgac tcccgcacca atgatcagcg
    12661 cgatgagaat ccatttgtat tgaaggacct gcggatcgaa caatgtggcc aagacggcca
    12721 agcccgaggc aatctcgccg atcagaacgc cgcgcatggc cgtaaccggt gaactgagcc
    12781 atttcagcgc caggatgaat ccgacggccg ccagaatgta ggtggaatcg atgagctgct
    12841 gagtcacgaa atcggtcatg gctttttcgc cttgccggtc ttgaacatat gtagcatgcg
    12901 gtcggtgatt acaaagcctc caaccatgtt gcagaacgaa gcggccaccg ccacgagccc
    12961 aagaatcgtg gacaatcggc tctcatgcct cccggcaatg ataatggccg caacgaccac
    13021 gatggcatcg agcgcgttgg tcagcgacat cagcggcgtg tgcaacaaag gcgaaacgcg
    13081 cttgatgacc tcaaatccca aaaaggccga caggataaac acaaacaaac tggaaatgaa
    13141 atccgctggc atccactcct ccttaagact tggccggcag ggcaggtagc gaaaaatact
    13201 cgcgtacccg aggattaatc acttcccctc cctgcgtcaa aattgtgctc ttgatgatgt
    13261 cgtcttctag attgacctga agctttccgt cttttactaa caactggagg aatgacgaga
    13321 tatttcgcgc gtacatttga ctcgcgtcat tgggaacggt agatgcaaag ttgaagtaac
    13381 cgatgatcga gacgccatgc gccattattt tttcgcctga cttggtcagt tcacaatttc
    13441 cgccgcgctc cgcagccaga tcgacaatca ccgagcccgg cgccatgccc ttaatcattt
    13501 cttccgtcac cagcacggga gattttttgc ccggcacgac ggcggccgta attaccacat
    13561 cgctttccgc gatcactttt ccaagcaatt cgcgctgacg cctgtagaat tcttcatcct
    13621 gcgcttttgc atacccgctg gcatcctgcg catccttggc tgcgattggg agctcgacaa
    13681 aacgcgcgcc aagactgtga acttgttctt tggaagcggg gcgcaaatca taggcggaaa
    13741 cgacagctcc gagcctccgc gccgtagcga tcgcttgcag accagctaca cccgcgccaa
    13801 tcacaagcac gcgggcggga gtgattgtgc ccgccgcggt tgtgagcatc ggaaaaattt
    13861 tcggaagcgt gtccgcggcg atcagcacgg ctttgtaacc gcacatcgtg cccatcgagg
    13921 agagcgcgtc catgctttgc gcgcgagtcg tgcggggcat taactccacc gaaaaggaag
    13981 tgacgcctga agcagcaatc tcctgcactg tttccagcga tcccatgggg cgcaaaaagc
    14041 cgataaggac ctgacctcgg cgaaagaggg gcaaatcggc gcggcccgtc tcgtcattcg
    14101 atccgtagca aagcatctgc acgatgatat cggcggaagc aaaaatttcg gctcggttca
    14161 cgataatctt cgcgcccttc gccgcataat cggaatcaag aaaaccagca gccgctccag
    14221 cgccagcctc gacgattact tccattccag ctttggcaag attgggaatc acactgggga
    14281 cgagcgcaac gcgacgctca ccgggatacg tttctcgagg aacacctaca atcattccac
    14341 ttcctcctct attccttaca gcgcggcaga taccaagcgc cggacaaacg gagacaatgt
    14401 ttttaatctg ccccaagcct aattcaagaa ttttcctcga gaaggccacg ccaggagaga
    14461 atatattcct tgtggcggcc gacgtggatt cgaagccgga aatgccgggc actatcgtgg
    14521 agcaactact gactcttttc agtgcagtaa caagcactga aataaggatt ttacatgcaa
    14581 aattacaaaa atccaattga agatcaaaag gctgtgtttc ccgagttgtg tcgatcatgg
    14641 attgcgatta agaaaggttt caataagccc aacgaaggga gggaccgatc gccactcctg
    14701 tagccataag aacgactacg tccggaattc gtgtccgccg gaatagccga attggtaggt
    14761 taagttcatg ccctaataca ctacactaag cgcaagactt tgctcattta tatgctcagt
    14821 aatcaaaaaa ccaaattccg tttgtatcta gattttccgg tacaattcga gattgacgca
    14881 cgcaacgcag ggggcaattt gaataatgct ttcatgcagg aagcgattga gcgggcggta
    14941 aaaaatgtgc gttccggtca gggcggtccg ttcggcgcgg tagtggtgaa gagtggccgc
    15001 gtgattgccg ctggcgccaa ttccgttacc acaacaaacg atcccacggc gcatgcggaa
    15061 gtgattgcta tccgcgaagc ttgccgcgct ctcggcacat ttcagctgac aggctgcgaa
    15121 atttatacca gctgcgaacc gtgtccgatg tgccttggcg ccatttactg ggcgcgccca
    15181 gaccgcgttt tttttgcggc caccgcaaac gatgccgcag ctgccggatt tgacgactca
    15241 tttatttatg aggaattgaa actgccacaa gagcagcgca aaatcccgct cgaacctctg
    15301 atgcgcgagg cagggctgga gccctttcgt gagtggacga ataagaacga tcgggttaac
    15361 tactgaaacg gaatcgcgct aaacgactga cgttttttgc ttgagttttt tgaataggtt
    15421 caaaggatat ggaagacaaa tattttggac attccgtcat tcgcaaggag gggcgcgaga
    15481 aagtcacggg ccgcgcccgg tacgtggacg acctgaccgt tccggacatg ctgcacggag
    15541 taaccgtgcg cagcaacatt ccgcgtggcc gcattcgcgc gatccacttt ggtgacggta
    15601 ttccgtggaa cgaatttaca gtcgtaaccg ccgccgacat ccctggaaaa aatgaagtcg
    15661 cgttgatcga acacgatcaa caatgcctcg ccgccgaagt tttcaatcat ccggaagaag
    15721 cgatcgtcct cctcgcgcat cctgaccggt atttgctgga agaagcgcgc cgcgcagtac
    15781 atatcgatgt cgaaccgctg ccagcaattc tcacaatcga agactccatc gcaaaacgcg
    15841 ccgttattta tggcgaggat aatgtcttca agtcttacca catggaaaaa ggcgacgtgg
    15901 acaccgcatg ggcgcaggca gacgttatcg tcgaaggtga atatcggacg ggagcgcagg
    15961 aacagctgta cattgaaact cagggcatga ttgcgaccgc gaattccacg gatggcgtga
    16021 ccgtctgggg ttcgctgcag tgtccatact atgtccacaa agctctggtt cccattttca
    16081 atcttcccgc ggataaaatt cgcgtcattc aaaccgagac gggcggcggc ttcggaggca
    16141 aggaagagta tccctcgatt atcgcggcgc atgcggcttt gctggctctg aaatcgggga
    16201 aacctgtgaa aattatttac gaccgcgcgg aagacatggt ggcgaccacg aagcgtcatc
    16261 cctcgcgcac gcggcaccgc acaggcataa cgcgcgacgg taaactcgtc gcgatggaca
    16321 ttgatttcgt gatcgatggc ggcgcgtacg ccacgctttc ttccgtggtg ctctcgcgcg
    16381 gtacgattca cgctgcgggg ccatattcct gccccaatgt gcgcgttcac tctcgcgcca
    16441 tggctaccaa tgccccgccg catggcgctt ttcgcggctt cggcgcaccg caaagtattt
    16501 ttgcgattga gcggcacatg gatcaactcg cgcgcgccgt aaatctctcg ccggacgaaa
    16561 ttcggcggcg caattttata ggcaaaggcc agacaacttc gaccgggcaa ctgattcacg
    16621 aggacgtaaa ttttaatgcc ctgctcgacc gcgcgtttcg ggaaagcaat taccacgcca
    16681 agcgcgaacg cttctcgcgc gaaaatcccg gcgcgaccat caagcgcggc atcggcttcg
    16741 cgactttcat gcacggcgcc ggctttaccg gttcaggcga agtgtatctg gcatccgtcg
    16801 tcgaattgga agcgacaccc gaaggaattg tgcgcgtcct cgccgccagc acggaaatcg
    16861 gccaaggaac caacacaatt ttttcgcaaa ttgcggccga agctctcggc ataggttatg
    16921 accgcatcga aattgcgcag cccgatacag gggcagtccc caacagcggg ccgaccgttg
    16981 catcacgcac gtcgatggtg gttggaaatt tggtggagaa ggccgcgctt gatctgaaac
    17041 aaaagttggt ggacagcggt ttgctatgcg acgcctgcac cgaaactgaa tttgtgcgcg
    17101 cgtgccgtcg ctacattgaa acgcgcggac cgctgcgctc ccaaagtgtt tacgagcctc
    17161 ccccgggttt gttatgggac gacaaaaaat atagtggtga cgcctatggc gcatatgcgt
    17221 gggctgttta tgtcgcggaa gttgccgtcg ataccgttac cttcgaggcg cgcgtgactg
    17281 acttcgtcgc gctgcaggag gttggcaaag tgattcatcc cgtgcttgcc gcgggtcaaa
    17341 tcgagggcgg agtggcgcag gccatcggca tggcgctcta tgaaaatgtt aactggcgcg
    17401 atggacgcat ggccaacgga caaatgacga attacatcat gcccaccact gcggacattc
    17461 ctccgattcg agtttttttt gaagagcagc cctatccgtt cggtccgcac ggagcaaagg
    17521 gaatcggcga actccccatg gacggaccag ctccggctat tgtgaacgcg attgaaaatg
    17581 cgattggcat tttcattcgc gaaatccccg cgacaccgga agtgctgatg gaagccatga
    17641 cggaaaaggc ggatgcgatt gcctgaaacg gttaagaaga ttgacatcga ttgcacggtg
    17701 aatggcgagc ggcggcacgt gcaggcattt cccatggcgc ggttgctcga cgttttgcgc
    17761 gaagaaatga atttgaccgg aacgaaagaa ggttgcggcg aaggcgagtg cggagcctgc
    17821 gcagtcctcg tcgatggcgc aatcgtgaac agttgcctcg tgcctgcgat cgaagccaat
    17881 gggaccgaca tccgcacaat cgaaggaatt gctcatggcg accagttgaa tgctgtgcag
    17941 gaagcgtttc tgcagtgcgg cggcgcgcaa tgcggaattt gcacgtctgg aatggtgatt
    18001 gcctccaccg ctttgctgga ggtgcatcct catcccaccg atgcggaaat tcgccagggg
    18061 cttgccggaa acctctgccg ctgcacaggc taccagaaaa ttttcgcagc ggttgtgcgc
    18121 gcttgcgaat caaccgccaa taaagatgca gggacgcgat gagagcttac attccctctt
    18181 atgatttgcg cgtgcccgga agtctttcgg aagcgctcgc gcttctggcc cgcgaaccgg
    18241 gggtttggca accgttcgca ggcggcaccg acctgatggt gctgctggaa gctggaaaac
    18301 tttctcacaa gcgattcttc agcgttgccc ggttggatga tttgcgcggc gtcgaaatga
    18361 ataccgatta tctgacattg ggcgcgctga ccacctacac ggaagttcag aagcatcaag
    18421 cgctccaaaa acaatttccg ctgctttgcg cggcagcgaa ggaaaccggc agcatcgcta
    18481 cacagaatcg cggcacgctc ggcggaaata ttatgaatgc ttcaccggct gccgataccc
    18541 cgcccgcttt gctcgtatac gatgcggaaa ttgagcttat atcgcagcgc ggtattcgct
    18601 ggctgcccta tcacggtttt cataccggat acaaaaagac gcgaattgcg cccgatgaat
    18661 tgttgcgcgc gatcaggctt ccctggcgca ctaaaaaatg gcggcaatat taccgcaaag
    18721 tgggcacgcg caaggcgcag gccatttcca aagtgtgttt cgcgggaggt gcgcttttgg
    18781 aaaacggcgc cattcaagat gtccggattg cactgggcag cgtggctccc gtcgttcagc
    18841 gtgcggtgaa aacagaagat gccctgcgcg gcaataggat cacaccgacg atgattgcca
    18901 cggcgaagga aatacttttg cgggaaattg cgcccatcga cgatatccgc tcgaccgccc
    18961 gttatcggtt gcgcgtcgcg caaaatctgc tcgaagaatt tcttttgcaa ctcgataaat
    19021 gacaacgacc cgcgcgattc gcagcaaacg agtggtgacg ccggagggag ttcggcccgc
    19081 aaccgtactc ttgcgcggcg gcttgatcga agccatctca ggctataccg atagttctgc
    19141 gggcaaaaat gttcttgatg caggagaatt cgtcgttatg ccgggcctcg tggacacgca
    19201 tgtccacatc aacgagccgg gccgcaccga ttgggaaggt tttgaatcag caacacgcgc
    19261 ggcggcagcg ggcggagtga cgacgttgat cgacatgcct ctcaatagta tcccggcaac
    19321 taccaccctt gcggccctcc gaacaaaacg caaagcggcg cgcaacaaat gctgggtaaa
    19381 tgttggcttt tggggcggtg ttgtgccggg aaataccgcg gatctatctg cgctgcatcg
    19441 cgcaggcgct ttcgggttca agtgctttct tgtgccttca ggcgtgccgg aatttgcaaa
    19501 tgtaaacgaa actgatttgc gcgccgcgtt gccagtcctc gccgagttga atgcgccact
    19561 gttggttcat gcagagttac cgggaccgat cgataaggct ggtgagaaac tcgcaaattc
    19621 cgatcccgcc aagtatgaga actggctgct ttcgcgtcct gccgttgcag aaaccaaagc
    19681 gattgagatg atcattcgct tggcgcgcga atgtagggcg cgggtacaca tcgtccacgt
    19741 ttcttccgag ctttctttgc cgctgattcg ccgcgcaaaa acgcaaggtg ttcgcatcac
    19801 agcggaaaca tgcccacatt atctcttttt tgattcatgg tccatcggga acggaagaac
    19861 agaatacaaa tgtgcgccac cgattcgaga tgcaaaaaat aacaaaaaac tcttgttggc
    19921 gttgagtcat ggcgagatca acttcgtggt ttccgatcat tctccatcgc cgccagcgat
    19981 gaaatgtctc gattccggca attttttcaa agcgtggggt ggtatagcat ccctgcagct
    20041 tgggttaccc gccatgtgga caacaatgcg tgcacaaaaa tattctctgg aacatttggc
    20101 gcactggatg tgcgccgccc cagcacagct tgctgggcta gataagagca agggcagtct
    20161 ggcgaaggga tacgatgccg acattgtgat ctggaatccg gataagagtt tcactgtgca
    20221 accaaaaatg ctgcaacatc gacacaaact tactccttac ttaaaacacc aactgcatgg
    20281 agttgtagaa acaacatttc tgcggggcga gatgatttat gatcgtggcc gatttctcgg
    20341 caagcccaaa ggggatctct tgcggcgagg cgggcgctga tggatttcac cgaatacatt
    20401 gatctcgcgg cagagcggct cggcgggcgc gtgcttgctg ccaatgacga attttttgcg
    20461 ccgaaggaaa atctattaaa agcatccgct cccgtttgga ttgcggacaa atatacggac
    20521 gtcgggaaat ggatggacgg ttgggaaaca cgccgccgca gaactccggg ctacgactgg
    20581 tgcattgtgc aactgggcct tcctggcgtc atccatggcg tggtggtcga cacctcctat
    20641 ttcaaaggta attatccgga acattgcgag attgaggcgt gcgctgtgga tggagatccg
    20701 agcctcgatc aactcgaaga ctccgcaacg cagtggcgaa acctgcttcc gaagtctcca
    20761 cttacgggag attcacgaaa tctatttcca cttgcgccat tggaaagagt tacgcattta
    20821 cgtttgaaaa tttatccgga tggcggcgtg gcgcgtcttc gcgtgcacgg cgaagtcgtt
    20881 ccggattggc cgtcgttaac tcgtgcaggc ggcgaaattg atctggccgc cgttgagcac
    20941 ggcggctggg tcatcgtctc gagcgatatg tttttcggct ctagaaacaa tttgattcaa
    21001 ccgggtcgat ccaccggaat gcacgatggc tgggaaacgc gccgtcgccg cggtcctggc
    21061 cacgattggt gcatcttacg ccttggcacg gcgggcaaaa tcagccgcgt cgaagtcgac
    21121 acatcccatt ttaaaggaaa ttttccggaa agttgttcgc tcgaagtttg cgccgcgccg
    21181 cgcgatcttg tcgatccgga caagctggaa actctgccat ggaaagaaat cctgcccagg
    21241 acgaaactaa aagcaagctc cctacacatt tacgaaaatg agttgcagca aatgggtggt
    21301 gccacacacg cacgcttcca tatttatcct gacggcggtg ttgcccggct gcgcctcttc
    21361 ggcaagcctg caacgaggtc cgcgtaagat gacgctcgcg gattttaatg cgcttccatc
    21421 caatcaagca gtatcgctgt tgatggattg ttgcggttcc acgcgttggg tggcgacgat
    21481 tgcggcacgc cgcccctacg cagatccgga ggaattgcac aaaaccgctg attccgtctg
    21541 gtggaaactc gatcgcgcgg attggcttga ggcattcagc catcatccgc aaatcggcga
    21601 caaacccgca agcggttcag aatccgctcg ccagtgggcc gaaggcgagc agaacggtgc
    21661 gcgcatcgca agcgacgacg taaaaactcg cttggcaaga gcgaatcgcg cctattttga
    21721 aaagttcgga tatatctaca tcgtttgcgc caccggcaaa actgccgaag gaatgcttgc
    21781 gattctgaat cagcgcctcc agaatgatca ggctagcgaa ctctcgattg ccgcggaaca
    21841 gcagcgttta atcacgcgca ttcgattgca aaaacttttg gccggagagg accgatcttc
    21901 atgagcgcaa tttccacgca tgttctggac acggctcgcg gctgcccggg cgctggaatt
    21961 cgcgtgcagc ttcaccgttt gtcaggggaa gcatggaagt tgatcggcga aagtcacacg
    22021 gacgaaaacg gccgttgcgg caacctgact ggaaacgaga agcccgaacc aggaacatac
    22081 cgcctgattt ttcatgcggc ggaatacttc gagaaccaaa ataccaaaac attttattct
    22141 gaaattcctg ttattttcga ggtcggggat tcggggcggc actatcatgt gcctttgttg
    22201 atcagcccgt tcggatattc tacttatcgc gggagctgat tcctggtttg gatgggagat
    22261 ttggcaatta aatttagaga aagctgccgg cgggacgccg gcgctacgaa atcccaccct
    22321 cttacttggt gcggtttttt aggattttca ggaatgagcc gaagtgtaat tcctcgatcg
    22381 tcactccggt caggcgctcg gcttccgctt cgctgattgc tccgcaatta atcgcgcgca
    22441 ggaaaaagtt cagcaagcct tgtcctgtcg ccgcatcgtc aaacacgtcg ccaaccagcg
    22501 ttgcctttgc cgctttctcg acaaaattct tgagccgcgc ggcggaggag tccaggcctt
    22561 ccagaaaaaa tgaaaaaaca gaggcatagc gtgttgggag ctgcgcggga tgcaagtccc
    22621 acccctgata ataggcggtt acaagcgaat gctggatgtg tccgtagtgc aatttccacg
    22681 cgcggtgcac ggcttcaaga ttttctttct gttgcgcgcg cgtaagtcgc tttccctttt
    22741 ccgcacggtg caacggagtc ggcattacat tcgttgcgcc gtctgaaagc caaatccctg
    22801 tgcccgcaaa cgcaacttgc atcatgttct tcgcaaagtc gcaggcagga tgatccatcc
    22861 tctggtaggc ggcagtgatg ctgcagcttg ccgtgtaatc gtaagtccca aaatgcgcgg
    22921 caatgcagcg gccgcctgcc gcatccagga attgcggcaa gctcgattct ccgcgagcgt
    22981 tgaggatcgc cgatgtcgtt tcgatcatca tctcaaatcg gagcgctcct ttttccaatc
    23041 ccaattccgg ctccatcgcc gcaagcaatt gcgatagagc tgccatctgt gcaggaatta
    23101 caacttttgg cagcgtaacg acgaaattcg cgggtaggcg tccccccgtg gcttcacaca
    23161 acgtgctcag gaaaatattc aaagtgcgaa tgctgcgatc gcgcaattcc ggcgaaaagg
    23221 gtttcagacg gataccaata aacggaggca gcgaattgtc tgcgaatccc ttggcaacct
    23281 gttgcgccgt gagtttcgcg tgctgatctt cttcctcgtc aggacgattt ccatagccat
    23341 cctcgaaatc aattcggaaa tcttccaccg gttctcgccg caatttttcg agcacgaggg
    23401 tgtatatctt ttgggccagc caggcaggcc tattcgtaag ccgcagtttt tcaggagctt
    23461 tcgcaaattg gcgttcagcg gcaacggcta ccttgccagt ttttgaaagt gtcgccgctc
    23521 ctggcaggcc tatagcgcgt gcgaagctga atgcatccgg cgcgaattct gcaagcgccg
    23581 ccaaagctcc ttctcccagc cgcctggaaa tattggagtg aaataaatga gcgcctccgt
    23641 aaacaacatg cactggttgg cggcgtcccg tttctcccgg gtatcgacgc gcgaacgccg
    23701 ccgatggccc cgccagaatt tccaacgcag atcgaatggc cgaatctgac aatgatgttt
    23761 tcattttcct aatcttccta atatttactt tagccaaaat tcactcagcc ggatttgcct
    23821 tgaaacaatc cagccaaaag aaaaaccacg ctcatagtaa acggcgggac tacaacattc
    23881 aatttccaac ggattgaacg cattaaagag aaaaagaaag ctaccagagg aaaaaaacag
    23941 acacacgaac gcacatgtcc ttacgcgggg taaagtccga tccgatcgtc acacgaagga
    24001 gaaaaattca ccgaggggaa tcacccaata gcggagggcc ctcgcgcttc cttatcgcgt
    24061 gctacgccgg ggcgctcatg gaccgccgct gctgaaagca ttcgcggcag aacacagggc
    24121 ggccctgcgt cgggcggaag ggaacagtag tttcctttcc gcactgcgag caggttgtct
    24181 tggtttcagc tctttggaat gtattggtac tcggacccgg actctgagag cgcttggctt
    24241 tgcagtcctt gcaccgcttg ggctcgtttt tgaagccctt gtcggcgaaa aacatctgtt
    24301 cgccggcggt gaagacaaat tcttgattgc attccgcaca cttcaggacc ttatcgtgat
    24361 attccataca ctactccgag gctccaagct ggttagagcc cacggcaccc cggccatgtc
    24421 agatattgca gggatacgcc ggggaagaac gtggatccca attccgactg gcctaaggtg
    24481 ctctggaaaa tcccgggtcg tggtgcgtgc ctgggggagg ttagtccaac tcccgccgct
    24541 gctttttgcg gagacgcttg cgcgatagcg cttgttttgc gcgtcgtttt tcgccaggct
    24601 tcatgtaata actgtgtttc ttaatttcct tgatgatgtc ttcctgttgc actttgcgtt
    24661 taaacctgcg cagcgcgttt tccagagact caccttcttg aatgctgact tcagccactt
    24721 agacttcacc gtccttccga tgcatgtggc aatgattgct attaaccatg aggagtatta
    24781 taaggcaaac ccgccctcta ttgcttattg gcactcactt ctagcggacc tgccttctga
    24841 agtcaagcgt tgtttttcgg acactcctaa ctgagctttc caacaatctc gggacagagg
    24901 ctatcgaact tttcggctca cctgctagaa tgacgaagca cacacctacg ctccaggtta
    24961 tatgggggca ggcgaagaga ttccctaatg tctttgatgc gtgacgcgat cggcgttctg
    25021 ctggcgggcg ggcaaggtga acggctgtgg ccgcttactc gtgaccgcgc caagccagcc
    25081 gtacctttcg gcggggttta ccgaattgtt gatataaccc tctcgaattg catcaacagc
    25141 gatctccggc gtgtcttcgt tctcacgcag tacaaagcgc tctccctcaa ccgtcatacc
    25201 cggcagggct ggtcgcaact actcgggctc ggcgaattca tcgaagtgct gccgccgcaa
    25261 atgcgcgttt ccaccaattg gtatctcgga accgccgacg ccgtttacca gaatatttat
    25321 tcgattggca gcgagcattc gcgttacgtc atcgttcttt ccggcgatca catttacaaa
    25381 atgaattacc agaaaatgct gcagcagcat gtggattcgg gcgcggatgt aacggtcgcc
    25441 acgcttgaag ttccttcaac agaagcagct gggcgatttg gcgtccttga aacagatgcg
    25501 aattggcgca tcgtcggttt cgaggaaaag cccgcggcac cgaagcgctc gcacctgcat
    25561 ccggacatgg tgaacgcttc catgggcgtt tacgtgttta acacgcagct gttaattccc
    25621 gcgctgttgg cggatgccga ggatccgaat tccaaacacg attttggcaa agacattttg
    25681 ccgcgcatct tctcgaaata tcgcgtgttt gcctacaatt tcatcgatga aaataaaatt
    25741 ggcccgcaat actggcgtga tgttggaaca atcgaagcct attacgaagc aaacatggat
    25801 ctggtttccg tgtcgcccat ttttaacctg tacgataaat catggccgat ccgcacctgg
    25861 cagcaccagt atccgccggt aaaaactgta ttcgcggatc ccggccgcat gggcgtcagt
    25921 atggattcga tggtgggcgg aggatcgatc atttcgggtg gacgtgtcac gcgctctgtg
    25981 ctgggctacg acgtgcgcac caacagctat tccgaagtgg agaattcgat tctcttcaac
    26041 catgtgaatg tagggcgtca cgcacgcatc cgcaacgcca tcatcgaccg ccacgtgacg
    26101 attcccgagc gcgccgaaat tggctacgac atggaagccg atcgccagcg ctaccacgtc
    26161 accgaaggcg gcatcgtcgt tgtcgtgcgc gaagaatcca tgatcgaaga accggaatga
    26221 gaaaggcagt tgagagtcga gagttaagag ttaaaaaaac ttgcgtgatt gggcgccaac
    26281 taaagccctt ccactcttaa ctctcaactc ttgactctga acttttttca atgctttttg
    26341 tccgtgttta tcagtgtgat ctgtggttcc aatcgattta gacttcctca ggaggcggca
    26401 tgtcgacgaa gatcgcgcgg gtgcatggca ggcagatcat cgattcgcgc ggcaatccca
    26461 cggttgaagc agatgtcatt ctggaaggtg gcgcggttgg acgagccgct gtgccctcgg
    26521 gtgcatccac cggcgagcac gaggccatcg aattgcgcga tggcgacaag tcgcgctacc
    26581 tcggcaaggg ggtgctaaag gccgttggca atgtgaatgg cgcgattgcc aaggctgtgg
    26641 caggcctcga tgcctctgac cagaaagctc tcgaccgccg tatgatcgaa acggacggat
    26701 cgccgaacaa gggcaatctt ggcgccaacg caattctcgc tgtctccatg gcagcggcgc
    26761 gcgcggcagc ggtggcggcg cggcaacctc tgtacaaata tctctcgcgg tactcgagcg
    26821 acccttccgg cagcacgctt cccgttccga tgatgaacat tttgaatggc ggcgcacacg
    26881 ccgataattc tgtcgacttt caggaattca tggccatgcc cgtcggcgca aagacgtttg
    26941 atgaagccct gcgcatgggc gtcgaggttt ttcatcgcct aaaaaccgta ctgaccaagc
    27001 gcgggtactc gacggcagtc ggtgacgaag gcggctttgc gcctaacttg aagtcgaacg
    27061 aagaagccat cgaagtgatt ctcgaagcca ttgcggcggc cggctacaag cccggtgagc
    27121 acgtggccat cgcgctcgat cccgcgtcca gcgagttcta cgagaaagcc agcggcaact
    27181 acgtgttcaa gaaatccgac aaatccgcgc acacgtccga gcagatgacc aggttttatg
    27241 ccgattgggt ccgccagttc cccatcgttt ccatcgaaga cggactagca gaagacgatt
    27301 gggccggatg gaagcacatg acggctgaat tgggcagcaa gacgcagctt gtcggcgacg
    27361 atttgttcgt gaccaacacc gtccgcctgc aacgcggaat ttctgaacat atcggcaatg
    27421 caatcctgat taaactcaat caaatcggca ccgtcaccga aacgatcgaa gccatcgaaa
    27481 tggcgcgcaa agcaggctac gcatcgatcg tctcgcaccg ctccggcgaa acggaagaca
    27541 cctttatcgc cgacctcgtc gtcgccatgg gcacaggcca aatcaaaacc ggctccgcct
    27601 cccgcaccga ccgcgtcgcc aagtacaacc agctcttgcg gattgaagaa gaactgggct
    27661 ccgccgcaaa atttccgggc aaagccgcgc tcgtacgaaa ataatctctg ttttttcaaa
    27721 acccaatgcc aaaacgaccg aaaccgattg tactaaccgt cctcgatgga tggggctacc
    27781 gcgtcgaaac caagggcaac gcgatcgctc tcgcgcgaaa accgaactac gaccgcctgc
    27841 tcaaggattt tcccaacacg ctaattcaga cgtctgggcc atttgtcggc ttgcctgaag
    27901 ggcaaatggg caacagcgaa gtgggtcatc tgaacatggg cgcgggacgc atcatccaca
    27961 tggacatcac gcgcatcgac gtgatgatcg tgaccggcga atttttcaag cagccgctgc
    28021 tcctcgaagc gatggaacgg ggccgcgctc gcaacttgca tttgttaggc ctcttgagtg
    28081 acggcggcgt gcactcgcac atgcagcatc tctacgcgct gctgcgcatg gcgcgcgagc
    28141 atcatgtgga gcgcgttttc attcactgct tcatggatgg ccgcgacacg catccgcaca
    28201 gcggcgtcga atatattcag caactgcaac agaagattcg cgaaatcggc gtggggcaga
    28261 tcgcatcgct cacaggccgc tacttcgcaa tggatcgcga caaccgctgg gaccgcgttg
    28321 agaaagctta tcgtgccgtc gtgcacggcg atgcgcccac aaaatcggcg gatccgctgg
    28381 aagccattcg tcgcagttac gaacgcggcg tgactgatga attcgtcgaa cccatcgcca
    28441 tcgcgagcgg cacttcgccg tccgcgccca ctgtcggccc agtccgcgat gatgacgccg
    28501 taattttctt caatttccgt gccgaccgcg cccggcaaat gacgcgcgct atcgccgagc
    28561 ccggtttcaa ggaattttcc gacatcaggc gccccacaaa tcttttttac gtggcaatga
    28621 cccagtatga aaagacatgg ccgtggctga gatacgtgat cgcgcccgaa aaaatcgcgc
    28681 acatcctcgc gcaggtcttc gccgagcgga atttaaaaaa cttgcgctgt gcggaaaccg
    28741 aaaaatacgc gcacgtcact tattttttca atggcggcat tgaaaaacct tacggcggcg
    28801 aagaacgcat tttggttccc tcgcccaaag ttcccaccta cgacttgaaa ccggaaatga
    28861 gcgcggtggg aattgccgaa acagttgtca aagctgtgga gaaagacgcc ttcgacgccg
    28921 tcgtcatgaa cttcgccaac gccgacatgg tagggcacac tggcagcctg gaagccgcaa
    28981 tcaaggccgt ggaagctgtg gatggatgcc tcggcatcat tcggcaagcc ttgcagcctc
    29041 gcggtggcgc gtggattatc accgccgatc acggcaacgc cgaaaccatg atcgacccgg
    29101 tcaccggcgg cccgcacacg tatcacacca caaacccagt gccgctgatt ctcgcgacgg
    29161 aaaatgaaat cggaccgctc gcgcccggcg gctctctgcg cgatgtcgcg ccaacgctgc
    29221 tcggcatcct aggcttcccc gtaccgcccg aaatgacggg acacgatttg cgtttgaaga
    29281 agtgaccagg acttggtgcg gggtgactct aatttcgtaa cccatctact ttagcgcgtt
    29341 gcagtagccc gcctcttcag aggcggggat tctgacgctc agccgttcac ctctaaaccc
    29401 tcgcctctaa aagtcgagcg gcaattctgc tatttcagat ttcgccgaga cctactgcgc
    29461 tccgcgctcc agtatttttt caaaaatttc tggtatttca aaaatggcgc ggttattcac
    29521 cgccgctgcg cgagcctgat attgcgcaag agcgcccggc tccagcaact tggccagtgc
    29581 ttttgcggtt tcgcgatgac tgtccaccac catgcccact tctttttcgc gcacccactg
    29641 cgcgttgtag cgttcctgcg gcaacgtcca ggcattgcaa tcgatgatca cgggcaggtg
    29701 cttggaaagc gcctcgctga tgcttccggg ccccggttta ccgatgaaga aatccgacag
    29761 cgccatgaaa tagggaattt gcgttgtaaa accctcgatg tgcagcggaa tgcgggaagg
    29821 ttcttgccgt agcgcctccg caagcttttc gtttcttcca cagattaata tcagctgcac
    29881 ctgaagtgaa gaccggttga tcctccgcac aatatctagc atcttccacg tgccgtatcc
    29941 gccgaataaa acgatccccg ttggcaaatc cggattgagc ccaagttttt ttcgctcggc
    30001 gctccggtcc acttccacag gctcataaaa ccgcgggtga agaatcatgc cggaggtccg
    30061 gaacacagtg ccatcgagat ggccaagctg gcgcgcttgc tccacggcat gttccgttcc
    30121 gcagacaatg tattgcgatt ctttttcgat ccagaaatgt ttcggataat ccgcgaggtc
    30181 tgtcagcacg gtgacaaatg gcctgccggg gcaagccttt tcgtatgctt cgcgcatggc
    30241 acgattgaaa tgaggcacaa gcgacaccac catatccggc gagcgcttct tccagaattc
    30301 ttccagcacg cgcaccgaag gcccgtgata cgaccgcacg accagctgca gaacgcgcat
    30361 taattgaggg ctgcccagcg tccagccatg ccgcagcatc ttgttataca tgtcctgaat
    30421 gcgaattccg gtcagtttgc gcagtatgtc cagaggatcc aatacttcct ggaggttaac
    30481 cattgatacg tcaatcggga gccctaactt ctctatttgc acctgtaaag cggtggctgc
    30541 gttgcggtgc ccacctcctg catcgaagaa aataagatcg atagcgcgtc tttggcccgc
    30601 tgggcgggac aatccatctg ccatcaatct tctcgcctcc ccaatcgtta acttgtttgt
    30661 gttcaagtag taacagacga ttgttacgag tttttaaaca ttgcgtaata agtcagcaaa
    30721 ctcattttgc gaaaattttc cgcaatgaga tgcgatctgc acgtccactc gctccactca
    30781 gggaccctgc ctgtggccct cctgcgccat ttttttctgg agagctatag tgctccgact
    30841 gacgtctact ccgtgctgcg caaacgcggc atggacctga ttactctcac tgatcacgat
    30901 tctatcaccg gcgcggaaga gcttcgccgc catcctgatt tttttgtcag cgaagaagtc
    30961 acttgcacca tgccgagcgg aacccaagct cacgtcggtg tttatgacat tacggagcgc
    31021 cagcacacag aaattaatcg ccggcgtaac gaaatggttc gcctgctggc ttacctctcc
    31081 gaacagcgcc tgttcttcac gatcaaccat attttttctt ccgtgaccgg aaagcgacac
    31141 acggacgact acgaatggtt ctccggctat ttccctgccg tcgaagctcg caacggccag
    31201 atgctgcccg cagccaacaa acttgccgcc gactttgcct ggaaacgatg taagacacag
    31261 gtggccggaa gcgacgcaca tgcgctggca tcggttggca cgacgtatac cgaagtgccg
    31321 ggcgcaaaaa acaagaaaga gtttttcgcc ggacttcgag tcgggcacgg caagctgtgc
    31381 ggcgaatcgg gcggctattg gaaattgacg cgtgatattt tgctgctgtg cggcgaaatg
    31441 atgcgtaagg atccctggac cgtgctgctc gccccgctcg ttcccctggt tcccgcgggt
    31501 gtcgccgtcg actatttcgt cgacctgagt tttatccgcc gctggggcac tgcgatcaat
    31561 ggccctcaac ccgcgccaaa ggacttactc gaaacactgc cgctgcgcca gaaacagatg
    31621 gcctaacgga taaagattgg aattgttggg ttgagagcct aaaggcagcc cgttttgaaa
    31681 aggagcagcc atgacagcac gaagagtggt ctgggagttt atccaagccc gtgactatca
    31741 ggtcatgagg aaagtccatc gatggcgagc gccacggtgg ctgcgttact ggatgattgc
    31801 ttccacaaaa ttgggagatg gctggatctg gtattccgtg gggatcgcgc ttcttttgtt
    31861 cggtggcgat atgcgattta tcgccttcgc cgccagcgcc tccgccgaag ccgcgaccgt
    31921 cgttattttc cgctacatca aaaaagtcag caaacgaaag cgcccgtgcc acatggaatc
    31981 gcactgctgg gcgaatatct taccgcccga tcaattttct ttcccctcag gtcacgccat
    32041 gagcgccttt gccatcgcca cgccgttatg catcttttat cctgagctgc aggcaacgct
    32101 gattgcgcta tcggtgagta ttgcattatc gcgggtaatc ttgggaatgc acttcgtgag
    32161 tgatgtggtc gtgggatcct tggtgggttt cggactaggg tatggcgcat acttgctagt
    32221 tcgctagccg caaaaattaa gtaactcgag tgacaacgag ccgaacaaga gaaaaaattc
    32281 tccgcgtttc tcctcttttc ctctgcgtcc tctgtgttca acgcttttga tcttgctccg
    32341 cctcaggacg caatccgttc gaaaatccca gccgcgccca tccccccgcc gatgcacatg
    32401 gtgaccattc cgtagcgagc attgcgtcga tcgagctctc gcaaaatcga cgccgtaagc
    32461 ttcgcacctg tgcatccgag cggatgcccg agagcaatcg cgcctccatt gggatttatg
    32521 cgcgcaggat caagccccgc ctcttgaatc accgccagcg actgcacagc gaatgcctca
    32581 ttcaactcaa tcacatccat ctgatccagc gtcaggccag taagtttcag cactttggga
    32641 atcgcaaacg caggaccgat tcccatgatt tccgcaggca ccccggccgt agcaaatcct
    32701 aaaaaacgcg caagcggctt agcacccagc gcacgcgcgc gttcagcgct catcacgatc
    32761 gtggccgcag cgccgtcgct cgtctgcgaa ctatttcctg ccgtggtcgt gccacgcacg
    32821 tgaaaagccg gcttgagttt ggcgagcgct tccaggctgg tatcagcgcg cggtccctcg
    32881 tccatatcaa aaagagtcgt ggtggtcttg gtttttgccc cacgtccatt cgcagtggtc
    32941 gcagcaactg tcgtctgcac ttcgaccgga acaatttctt cgttgaattt cccggccgca
    33001 atcgccgcga gtgctttcat atggctggcg agagaaaact cgtcggcttt ttcgcgtgtg
    33061 atcccgtatt tcttcgccac attttctgct gtcagtccca tgctcaggta agcgtccggg
    33121 tagtggtcca tcagccaggg attcggcgag actttgttgc cacccatcgg aatcatgctc
    33181 atcgattccg tgccgcccgc caaaatgacg ccggcgtgcc cgaccatgat gcgctcagcc
    33241 gccatggcaa tcgcctgcag ccccgaagaa caaaaccggt tgatggttat ggccggaacc
    33301 tcgggcggaa gccccgcgcg ctggctggca atgcgcgcaa tgttcatccc cgcctcacct
    33361 tcaggcatag cacaaccgag aatcacatcc tcgatgtccg caggattcag ccccggcacg
    33421 cgccgaatgg cctcctgcat ggcaatggca gccagatcat ccggtcgcgt ggaacgcagc
    33481 gttcccttga atgcctttcc taccggagtg cgtactgaac tgacgatgac agcgtctatc
    33541 ataaagtaat ccgtgactgg caaatgaatg ccggagactt ataacctgga tttgtgattc
    33601 ctaactcatg ctgtgcgcaa aacattggat gcttcgaaaa gtgaaaaagc cgcacgatcg
    33661 agtcatccaa aggtagcacg atgcctctat cttgttgtga actctggacc tgcatgctcc
    33721 gcaacgatgc gaaagaaaat attaggaagc tactgctcag cgtctaggcc acttgtccgg
    33781 cggggtgttt attctttccg ctccactggt acacggaaaa aatgctggcc gcgattgcaa
    33841 tcacaatcag cacgatcatt gcgtcttcca ggaccggtga tttctcctgc ctgacgatcc
    33901 agcgtccata gtgaaccgcc agcgctccct caatcagaaa gcgcgcaaac ctgcccacac
    33961 ccacaaaact aaagagtttt ttccggggat atttaaacgc ggccgctgcg gccacaaccg
    34021 ccgtaaaagg gaatggcggc ggcaaaaggg acgcggacaa aagtgtccac gctgcattct
    34081 tttgcaccag gtcttgaaca cgacctaagc gcatacccga tgaagattcc ttcaaccgtt
    34141 cgccgccttt tcggcttaac caaacggtag ccaggcagcc aatcacggaa cctagcgtcg
    34201 ccatcaaggc gtagtaaatc atgcgatcgt gatgacgcgc gctcaatgcc agaatcaata
    34261 aatcattgcc aaacggcaaa acgaggggcg aggaatcaaa cactcccaat gcaagtattc
    34321 ctagtccgcc tgcacgatat aaagcccttg cgataatgcg gatggctagt ctcatgagat
    34381 tttcgcctgc caagtatatg atgccggcag cagtggttca taggcccgcg ggttccgcaa
    34441 agcgggcggt gcagtcctgg attttttgcg tgcccgcgac cctcgattgc gagcccgctt
    34501 cataagatgc gtggattttt tctttgctcg gccagccgat aaatgcgctc aagattgcca
    34561 gttgatatcc tcatgctatt ctagagcgtt tttgatttaa gtgcacacat atcccgaaga
    34621 agcgaagtag tgtgtgcact ctgtcgagga gaccgtgttg aggacttgac cgattttctc
    34681 ccagagggag tcgaccgtgc gcgtggcggc ttgccgaagc cgcgctttga gtttggcaaa
    34741 gaacttttcg atggggttca ggtcgggcga atacggcggc agatagagca gcgtggcctg
    34801 cgccgcagtg atggcttctt tcactccagc caccttgtgg ctgctgaggt tatcgaggat
    34861 c
//
LOCUS       DASZMB010000030         8029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2783633, whole genome shotgun sequence.
ACCESSION   DASZMB010000030 DASZMB010000000
VERSION     DASZMB010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8029
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2783633"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1078)
                     /locus_tag="VG272_01240"
     CDS             complement(152..1078)
                     /locus_tag="VG272_01240"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HWF12332.1"
                     /translation="MMRTLKFLMFLFCLAALPAFSQGPPPSFAPPQLDDLVSRIALYP
                     DPLIAQILAGATYPDQIPDAARWADQHHYLTGQALADAIQGDQLPWDPSVQALLPFPS
                     VLDMMASDMRWTADLGNAFLGQEGDVMDAVQRERRKARDYGYLRSNGQVVVGGGPYIT
                     ITPVNPGYVVVPYYDPRVVYYAPRPGFYAGGAIRFGYGISLGLSFRPWGWGYNRFDWG
                     GHGVFINNARWGRTWNNRGGYVHPYEGVHRFGPGERVQDHHALEQRSEHERSFEREGR
                     RAPEEHHDRGHNERGHDDHGHGDGRDGGHDRH"
     gene            1311..1859
                     /locus_tag="VG272_01245"
     CDS             1311..1859
                     /locus_tag="VG272_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWF12333.1"
                     /translation="MAPQNKSAAAPHVVDASARIGHVHLKVADLKRALEFYSGVLGFE
                     VMQRLGDQAVFLSAGGYHHHIALNTWESLGGSPPPMGSTGLYHTAVVYPTRAALATAL
                     RRVLAAKISLEGAADHGVSESIYLRDPDDNGVELYCDKPKEAWPRDASGNLAMYTRML
                     DLNKLLSEAPSGSAVSTGPEDL"
     gene            2048..3547
                     /locus_tag="VG272_01250"
     CDS             2048..3547
                     /locus_tag="VG272_01250"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HWF12334.1"
                     /translation="MIRRDFCKSSLALAGLALSKGPADSLARPAAPIPADEMAKAPGL
                     TKYVSDFIVNTKYEDIPENVIAIGKKTMLDGFGLALAGSSSPSGPLIRQYIESLGPCG
                     GGASIIGTKLKVHPRFAALANGISIHADDYDDTGSAFHVASPVLPPSFALCETGKRSG
                     KDLMLAFHVGVEVGNKIGDAMSPRHQQEGYHTTGTIGTMGSAAACAKLRGLDEAKTAI
                     ALAIAGSEASGLRDNFGSMTKPFHAGHAGESGTVAVDLAAIGWTAAADILEAELGFFA
                     VGGGFDPKFIVDRLGKPWMFASPGDLIKRFPCGTIQQQVMDEMLRLIQANNIKAADVE
                     KIEVGGNLSNYRTLFQHHPTTGLQGKFSMEYAMALLLLDGKATLDSFTNAAAQRPDMQ
                     DMIKRVRYYVDPEFNKLELQGASLQAMLVEQSILKIYMKNGKVISGKTEPAKGSPENP
                     MTFEEVADKFRGNADFLKWPSQKAETVIAFVKTLESAPDMSRLTAALAG"
     gene            3731..4645
                     /locus_tag="VG272_01255"
     CDS             3731..4645
                     /locus_tag="VG272_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12335.1"
                     /translation="MIRQLKIFVAACLSACCFAIAFSSLPMSAADDEIISLDKAATAA
                     LSSADKAAPAKYLDSDFSWIDTKGVMRFGDDSFRAGIKPAVTASDDVKYIEHKYGNVV
                     WLQANEGKRYSAHFWVKRPAGWKLLHITEIDVHERDFSTVRPNYEVPCINPCKAVPYT
                     PTTANEKAALDGWQEQESGKDGMWAKHIADNFDQRAAMTWGGPRASKTEMVAMQARAK
                     QQNAGRPEVGTAPALFIRSWDFGTAVVMISCQPTYGDKAYWSSRVLAPLNGVWMMMES
                     YHNYIDASPIMTAVPIDQSKDPRAAAMK"
     gene            complement(4659..5546)
                     /locus_tag="VG272_01260"
     CDS             complement(4659..5546)
                     /locus_tag="VG272_01260"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWF12336.1"
                     /translation="MVIFLTGATGYIGGVVAQALQQAGHHVTGLARSPEKAKHLESRG
                     IRAHLGDLLQPETIASAARVADGVIHTANTNDANSAQADLAVVRAIVAALEGSGKPFI
                     YTSGVWVLGSTGDEVATEETPVNPTPLVAHRTAIEQEVLAAQARGVRAIVIRPALVYG
                     RGGSIPAMLTKSARETGATRYVGDGSNRWPMVDVEDLAQLYVRAVEKAEPGSLYNASH
                     GPSYRVAEVAAAASTGAGANGKTESWPLEEARKAMGPFADALVLDQQVSGEKAKKELG
                     WAPRAGSVLDDLKTGSYAR"
     gene            complement(5687..6010)
                     /locus_tag="VG272_01265"
     CDS             complement(5687..6010)
                     /locus_tag="VG272_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12337.1"
                     /translation="MILALVLIPGIIGMALLLRPWPFFLAPEQTQSYRDRNVAVEFVL
                     WVEKQARARGLSVRGVARPIPLECKKCGKSSNYFVYPDEVCERCWQASLKPITPQAKG
                     HHPSA"
     gene            complement(6242..7180)
                     /locus_tag="VG272_01270"
     CDS             complement(6242..7180)
                     /locus_tag="VG272_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12338.1"
                     /translation="MSRNPLIAVITVFVGLTTPTLLAAQNSSATAQGNQPKADKWNSI
                     PPVKPWYAGKKSGPAPRRDISGIWDAAELDGGRQPSGALEHPALTAPRGKGVEGGQQD
                     ETGIMRPLHYTPMGLEALKANKPSGPSVRQVPSAMANDPVDQCDPIGFPRMELFELRT
                     IELVQTANQVIYLNQFYGNWRVIWTDGRELPKDPDPRWNGYSVGKWVDDYTFVVETIG
                     LNPRSWLDHAGRPHSEDLRVEERFHRVDLDNMELTVTIYDPKMYTEPWQGLKTFPLHL
                     QPADFDISELICSPVDMAEYNRQVGNTVAAPANKTK"
     gene            complement(7399..7794)
                     /locus_tag="VG272_01275"
     CDS             complement(7399..7794)
                     /locus_tag="VG272_01275"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HWF12339.1"
                     /translation="MRNSLKITAVAILGLLAVSVPVMAHHGNAAYETDKSVNVKGTVT
                     AYIWSNPHVFVKVDMKDDKGNVVNWIVEAQNPVSMMQIGWTKNTFKPGDEVEIDAMPA
                     KNGNPVGFLGSSSPTAPRRRIVINGKQFQ"
BASE COUNT         1797 a         2343 c         2117 g         1772 t
ORIGIN      
        1 cttcagcctg tctgctttta atttcggaac cagggcgtcg accgagttct cggacagccc
       61 caaattcaaa accagacggg ttgaaacctg tcctacttaa ggcaggtgcc cgcccgaagg
      121 cggcctctac acaaataaaa catatggcgt tctaatgccg gtcgtgtcct ccatcacgac
      181 catcgccgtg tccgtgatcg tcgtgcccgc gctcattgtg tccgcggtca tgatgttctt
      241 ccggtgcgcg gcgaccttct cgttcaaagc tgcgttcatg ttctgagcgt tgttccaatg
      301 cgtgatggtc ctggacgcgt tcgcctggtc cgaaccggtg cacgccctca tacggatgaa
      361 cgtagccgcc gcggttattc caggttcgcc cccatcgagc gttattgatg aacacgccgt
      421 gcccgcccca atcgaagcgg ttatagcccc agccccaggg acggaaagaa agtccgagcg
      481 aaatgccata accgaagcga atcgcgccgc ccgcatagaa tcctggccgc ggagcatagt
      541 acacaacgcg cggatcataa taaggaacga cgacatagcc gggattgacc ggcgtgatcg
      601 taatatacgg cccgccgccg acgaccactt gtccgttgga gcgcaagtac ccgtagtcac
      661 gcgctttccg gcgttcgcgt tgaacggcat ccatcacgtc gccctcctgg ccaagaaacg
      721 cattgcccaa gtccgcggtc cagcgcatgt cggaagccat catgtcgagc accgaaggaa
      781 atggcaggag cgcctgtacg ctgggatccc agggaagctg gtcgccttgg atcgcatccg
      841 cgagtgcttg gccagtaagg taatggtgtt gatcggccca ccgcgcagca tcgggaatct
      901 gatccgggta ggtggccccc gccaaaatct gcgcaatcag gggatcgggg tatagggcaa
      961 ttcgcgacac cagatcatcc agttggggcg gcgcaaaaga tggaggtgga ccctgcgaaa
     1021 atgcaggtaa tgcagctaaa caaaacagaa acataagaaa ttttaaggtg cgcatcatga
     1081 tctcccggca tctattctaa atgccttgtt ttgtcgattt cctagagcaa acttgttatt
     1141 ttccgttgca gaaaatggta gcgcacatgg ttcggggatg tgtcgaaaga attgggacag
     1201 gttgccgttg cggcacctga accaaacaac agccgcttga ctctcctcca gggctggcgg
     1261 aaactctgca gagatcgctt tcactcaatt tcaataaacg gtgaaccgtt atggcgcctc
     1321 aaaacaagag cgctgccgcg cctcacgtgg tcgacgcgtc ggcccgcatc ggccatgtcc
     1381 atttgaaggt cgccgatctg aaacgtgcgc tggaatttta cagcggcgtc cttggctttg
     1441 aggtaatgca gcgccttggc gatcaggcag tatttctttc cgctggcggc taccaccatc
     1501 acattgcgtt gaacacctgg gaaagcctcg gcggttcgcc gccgcccatg ggttccaccg
     1561 ggctttacca tacagccgtc gtatatccga cgcgtgccgc gctcgccacc gctttacgcc
     1621 gcgtgcttgc ggcgaagatt tctctggaag gcgccgcgga tcacggtgta agcgagtcca
     1681 tctatttgcg ggacccggac gataacggag tggaattgta ttgcgacaag ccgaaagagg
     1741 cgtggccccg cgatgccagc ggcaacctgg ccatgtacac gcgcatgctg gatctgaata
     1801 aattgctgag cgaagcgccg tccggttccg ccgtctcgac gggtccggaa gatttgtaat
     1861 gtagagcccg ctttcaggcg ggcacattcg cgtcacctat atgcccggat gcccgcccga
     1921 aggcggacgc cacttgaaac aagttttttc acaacagcct tcagcctatt gctctggtgg
     1981 cacgcacgcg aaattaaaat ccaacgggcg caggcatgtt ctagtaattt tctacagggg
     2041 aattccaatg attcgacgtg atttctgcaa atcttcgctg gcgcttgccg gattggcttt
     2101 gagcaaagga cccgccgact cgctcgcccg tccagcagcg cccatccccg cggacgaaat
     2161 ggccaaggcc cccggcctga ctaaatacgt ttcagatttc atcgtgaaca cgaagtacga
     2221 agacatccct gaaaatgtga tcgccatcgg caagaaaacc atgctggatg gattcggcct
     2281 cgcgctcgca ggatcctctt cgccgtccgg tccgctcatc cgccaataca tcgaatcgct
     2341 gggtccgtgt ggaggtggcg ccagcatcat cggaacaaaa ttgaaagtgc acccacgctt
     2401 tgccgcactg gcgaatggca tctcgattca cgccgatgat tacgacgata cgggctccgc
     2461 atttcacgtt gcttcgcccg tgcttccgcc ctcgttcgct ctctgcgaga caggtaagcg
     2521 cagcggcaag gacttgatgc tggcgtttca cgtgggcgtc gaagtgggaa ataaaattgg
     2581 tgacgccatg tctccccgcc accagcagga gggttaccat accaccggca cgattggcac
     2641 gatgggcagc gccgcagcct gcgcaaaatt gcgcgggctg gacgaagcga agacggcgat
     2701 cgcgctggcc attgccggct ccgaagccag cggcctgcgc gataatttcg gctccatgac
     2761 caagccgttt cacgccggcc atgccggcga aagcggaacc gtggccgtcg atctcgcggc
     2821 catcggctgg acggctgcgg ccgatattct tgaggcggaa ctcggatttt tcgcggtcgg
     2881 cggcggcttc gatccaaaat ttattgttga tcgactcgga aaaccgtgga tgttcgcgtc
     2941 acccggcgat ctgatcaagc gttttccctg cggcacgatc cagcagcaag tgatggacga
     3001 aatgttgcgc ctgattcagg cgaataacat caaagccgcg gacgtggaaa aaatcgaagt
     3061 cggcggaaac ctcagcaatt atcgaactct gttccagcac caccctacga ccggcctgca
     3121 gggaaaattc agcatggaat acgccatggc tctcttgctg ctcgacggca aagccactct
     3181 cgattcgttc accaacgctg ccgcgcagcg tccggatatg caagacatga tcaagcgcgt
     3241 gcgctattat gtcgatcctg aattcaacaa gctggaactt cagggcgcgt ccctgcaagc
     3301 catgctggtt gagcagagca tcctgaaaat ttatatgaag aacggcaaag tcatttccgg
     3361 caaaaccgaa cccgcgaagg gaagtcccga aaatcccatg actttcgaag aagtcgcaga
     3421 caaattccgc ggcaacgctg actttctaaa atggccgtcg cagaaagcgg aaaccgtcat
     3481 cgcattcgtg aagacactcg aatccgcgcc cgacatgtcc aggctgaccg ccgcattggc
     3541 aggttgaaat ttatcgagta atcaggcaca gcgcccttgc tgtagcccgc ctcttcagag
     3601 tcgggacagc agtcggagca agcagtccgt ggaacaattc ggattgcgat gggagcaggc
     3661 agcttctgaa aaagttttcg ttctaaatta cgcacgcgaa aaacactttt ccttattggg
     3721 agaatccaaa atgattcgac aactaaaaat ttttgtcgcc gcatgcttaa gcgcgtgttg
     3781 ctttgccatc gccttttcca gcttacccat gtccgcggcg gacgacgaaa tcatctccct
     3841 cgataaagcc gccaccgcgg ccctctccag cgctgacaaa gccgcgcctg caaaatatct
     3901 cgactccgat ttcagctgga tcgatacaaa gggtgtgatg cgctttggcg atgattcctt
     3961 ccgcgccgga atcaagcctg ctgttaccgc ttctgacgat gtgaaataca tcgagcacaa
     4021 atacggcaat gtcgtctggt tgcaggccaa cgagggcaag cgctactctg ctcatttctg
     4081 ggttaagcgc cctgcaggat ggaagcttct gcacatcacc gaaattgacg tacacgaacg
     4141 cgatttttca accgtgcgcc caaattacga agtgccctgc atcaatccgt gcaaagccgt
     4201 tccctatacc ccaacgacgg ccaatgaaaa agcggctcta gatggatggc aggaacagga
     4261 atccggcaag gacggaatgt gggccaaaca cattgccgac aatttcgatc aacgcgcggc
     4321 gatgacgtgg ggcggaccga gagcgtccaa aacggaaatg gtggcgatgc aagcgcgcgc
     4381 gaaacagcag aatgctgggc gccctgaagt cggcaccgct cccgcgctgt ttatccgctc
     4441 atgggatttc ggcacggctg tcgtcatgat ttcgtgccag cccacgtatg gcgataaagc
     4501 gtactggtcg agccgcgtgc ttgctccgct caatggcgtg tggatgatga tggagagcta
     4561 ccacaattac attgatgctt cgcccatcat gaccgccgtg cccatcgacc agtcaaaaga
     4621 tccgcgcgca gcggccatga agtaaaaaat ggaaaaactc agcgtgcgta agatccggtt
     4681 tttaaatcgt ccagcacgga tcccgcacgg ggcgcccagc ccaattcttt ttttgccttc
     4741 tcgccgctca cttgctggtc aagcacgagc gcatctgcaa atggccccat cgctttgcgc
     4801 gcctcttcca gcggccacga ttccgttttg ccatttgcac ccgcacccgt gctggcggct
     4861 gcggccactt cggccacgcg gtaagacggc ccgtgcgacg cgttataaag tgatcccggc
     4921 tcggctttct cgacggcgcg gacatacagt tgcgcaagat cctcaacatc caccatcggc
     4981 cagcggttcg atccatctcc cacgtagcgc gtggctcccg tttcgcgcgc ggattttgta
     5041 agcatcgccg gaatacttcc gccgcgtcca taaactaatg ccggacgaat cacgatggcg
     5101 cgcacaccgc gagcctgcgc ggccaacact tcctgctcga tggcggtccg gtgcgcaacc
     5161 agcggcgtgg gattcacggg cgtttcttcc gtcgccactt catctccggt cgaaccgagc
     5221 acccacacac cgctcgtata aatgaacggc ttcccgctcc cctccagcgc agcaacgatc
     5281 gcccgcacga cggcgagatc ggcctgagcg gaattcgcgt cgttcgtgtt cgccgtatga
     5341 atcaccccgt cggcaacgcg cgccgccgaa gcaatcgttt cgggttgaag gagatcgccg
     5401 aggtgcgccc gaatcccacg cgattccaga tgcttggctt tttccgggct tcgcgcaagt
     5461 cctgtcacgt gatggcccgc ctgctgtaat gcctgcgcga caactccgcc gatgtatccc
     5521 gtagcaccag tcaaaaaaat caccatttgt tttttcctcc gtcagaaaac attgtactgc
     5581 gcacgcgagg gagtacaagt ggcggaaagg cccgatagga tgcaggttca cgaatgaagg
     5641 gaattaggca gcggaggaaa gattgggctg caggagacca gccaatttat gcagacggat
     5701 gatgtccctt ggcctgcgga gtaatcggtt tgaggcttgc ctgccagcag cgttcgcaaa
     5761 cttcgtccgg atacacgaaa tagttggaag acttcccgca ctttttgcat tccaacggga
     5821 ttggccgcgc cacacctcga acgcttagcc cgcgcgcgcg ggcttgcttc tcgacccaca
     5881 atacaaattc cacggccacg ttccggtcgc gatacgattg tgtttgctct ggagcgagaa
     5941 aaaaaggcca cggccgcagc agcagcgcca ttccgataat tcccgggatc aggactagcg
     6001 ccagaatcat ttgcggcctc acttctgaaa ctgtgttgac aacgtctaac tccacattaa
     6061 ctttgatgcg gcagcgtgca cgaacgatct taaaatttcg atacttatta gttactgcat
     6121 aattattttt gcggatccta ccgtgaggcc tgtcttcgaa tacaggagtt tcggaagagc
     6181 gatccttcag gagccacctg acaagttttt aggttcgact gaggtcatta attcgggaga
     6241 attactttgt cttatttgcg ggagcagcga ccgtattccc aacttgcctg ttgtattccg
     6301 ccatgtcgac gggcgagcag atcagttccg agatatcgaa gtcagctggt tgtaagtgga
     6361 gaggaaaagt tttcaaacct tgccagggtt ccgtatacat cttcggatcg taaatcgtta
     6421 ccgtcaactc catattgtcg aggtccacgc ggtgaaatcg ctcctcaact cgcaagtctt
     6481 cgctatgcgg acgtcccgcg tgatcgagcc agcttcgtgg gttcagtcca atcgtctcca
     6541 cgacaaacgt gtaatcgtcc acccattttc cgacagaata tccgttccat cgcgggtccg
     6601 gatctttggg aagctcccgc ccatccgtcc agatgacgcg ccagttgcca taaaactgat
     6661 ttaaataaat tacctggttc gccgtctgaa caagttctat cgtccttaat tcaaaaagct
     6721 ccatgcgcgg gaatccgatc ggatcgcact ggtccaccgg atcatttgcc attgcggagg
     6781 gaacttgtct gacactcggc ccggacggtt tgtttgcctt gagggcttcg agtcccatcg
     6841 gcgtgtaatg cagtggccgc ataatccctg tctcgtcctg ctgcccacct tcgacacctt
     6901 ttcctcttgg cgcagtcagc gcgggatgtt ccagcgcgcc gctcggttgc cgcccgccat
     6961 cgagttccgc cgcatcccag atcccggaaa tatcgcgtcg cggcgcaggt ccagattttt
     7021 tcccggcata ccatggcttc actggcggga tgctgttcca tttgtcagct tttggttggt
     7081 tgccctgcgc ggttgccgat gaattttgcg cggctaaaag tgtgggagta gtcagcccga
     7141 caaaaacagt aattaccgcg atcaagggat ttctggacat ttcgaaaatt cctccggcag
     7201 caacgaaaaa gagtcagtgc gcgcagttct aaaatcgcag tctaaaatcc ttgtgggtcg
     7261 tggctttagc caagacatta ttttgcccaa gcgaatccgg ctttagccgc tgtgcgttca
     7321 agttggaagt tctcgtgaag gtccactacc cagcttccca cgaaggtgct ccagattctc
     7381 gccatttcaa gtggagtcct actgaaactg cttcccatta atcacaattc gacgccgcgg
     7441 cgccgtcgga ctggaactgc ctagaaatcc aaccggattg ccattcttag ccggcatcgc
     7501 atcaatttcc acttcatctc caggcttaaa cgtattctta gtccacccaa tctgcatcat
     7561 ggacaccgga ttctgcgctt caacaatcca attcaccacg ttgcccttgt cgtccttcat
     7621 gtcaaccttc acgaatacgt gggggtttga ccagatgtag gctgtaaccg ttcccttcac
     7681 gttgacggat ttgtccgttt cgtaagccgc gttaccgtgg tgcgccatga ctggaacgga
     7741 aaccgccagt aaaccgagaa ttgccacggc cgttatctta agcgaatttc tcattgcact
     7801 ctcccaacgt gtgcgtgtgc caggttcgga ctccgttttt cgtgaatcga aatagatttt
     7861 taaattgtct tcgatcttga ccgccccgat acgtaccttc atacacccga attcggccca
     7921 gcgaaacaat aatgtacgcc cgcgcaaatt caccacggca tcgttcacga cggaagccca
     7981 cagtcaacaa cttaaattgg aaccctttgt ggaatccgca ctctaattt
//
LOCUS       DASZMB010000031         8671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_21755, whole genome shotgun sequence.
ACCESSION   DASZMB010000031 DASZMB010000000
VERSION     DASZMB010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8671
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21755"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VG272_01280"
     CDS             complement(<1..614)
                     /locus_tag="VG272_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWF12340.1"
                     /translation="MAKLPGTIEHTPLRWNIGRFAFTLEWRNRLQVSNNSRHETSPGA
                     QMNLPKRPLGSSGLQITTVGFGAWAIGGGGWAFSWGPQDDDESIVTMRRALELGVNWI
                     DTAAVYGLGHSEEVVGKLLRNLPSSERPLIFTKCGLTWDPNNPMQQAKRVLKPETIRR
                     ECEASLRRLGIERIDLYQFHWPDEMGTPVEDSWAEMVKLIEEGKV"
     gene            954..2072
                     /locus_tag="VG272_01285"
     CDS             954..2072
                     /locus_tag="VG272_01285"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF12341.1"
                     /translation="MRNRRDFIMSVVGASAGVVFTGCSICDAMAGARPPQVGSGKKHK
                     PVIVGNRRVKTVDIHCHVSVPEVTDLLKGTKIERRLGAGLAIGGYDDAGLTPARIQTM
                     DEMGIDIQAVSINSFWYSAERELVTKLIDLQNQKLAELCATHPDRFVAFASVALQFPE
                     LAAEQLEVAMKKYGLRGVGIGGSCDGKELSSPEFDPFWAKAEELQALVFIHPQDSANA
                     TGIRNRVQGNGVLSNVIGNPLETTMCLSHLILEGTLDKFPNLKICAAHGGGFLPSYAG
                     RIDQGCRVFPKECDKTTKMKPSEYLKKIYVDTIVFNQEALRHLIAVVGAGQIGLGTDY
                     PFPWDFAPVDYVMNQPGLSDADRIAILGGTMSKLLKIS"
     gene            complement(2158..2985)
                     /locus_tag="VG272_01290"
     CDS             complement(2158..2985)
                     /locus_tag="VG272_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWF12342.1"
                     /translation="MLKRTIPSTGEQLPIIGIGTYKGFDVGSGGDARAEVGEVLRTLY
                     SAGGSVVDSSPMYGRAEEVAGDLIAEQGVRGKTFVATKVWIEGRSAGIEQMKKSMKLL
                     RCTQVDLMQIHNLLDWRTHLATLRAWKQEGQIRYLGITHYNASAYADLEKIMRDETLD
                     FVQLNYSLDERDAEQRLLPLALDRGIAILANRPFGQGSLHRGLRGKALPAWAREAGCD
                     SWSELLLKFTLSQKAVTCAIPGTGNPAHMAENCRAGDGPMLDKSVVKNLEAYWDGLK"
     gene            3299..3580
                     /locus_tag="VG272_01295"
     CDS             3299..3580
                     /locus_tag="VG272_01295"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HWF12343.1"
                     /translation="MLLSRDYVGYMAGEVVKRLLAAKVIETPDAASVTGRVRAAMQDE
                     ISIEDRLNEEVREILTKHADEMRRTGVQYQEMYKKVKGELARQRKLILR"
     gene            3627..3923
                     /locus_tag="VG272_01300"
     CDS             3627..3923
                     /locus_tag="VG272_01300"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
                     /protein_id="HWF12344.1"
                     /translation="MRLTREKSIRLSHVITDVLVACDNVEFIDDRDTIRQKALQILQD
                     LLKEEEAVDAEVRKKISSQKKEILEASEEWDVLYRKYYADEMRRMGIVHLPSTS"
     gene            complement(4063..5331)
                     /locus_tag="VG272_01305"
     CDS             complement(4063..5331)
                     /locus_tag="VG272_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWF12345.1"
                     /translation="MRRLSSGLIAIIFAAVVGAPTGYAAPGQAQQPQSQPQPQTQTQT
                     PTTTDPPQTPPTQEEATPPASDEDATAKGVKPGSDKDVNAIGNRGVGNGVNLYSLQRE
                     IALGKQAAMEVGKTARMINDPVVTEYVNRVGQNLVRNSDAKVPFTIKVIDSDEINAFA
                     LPGGFFYVNSGLILRADEEAELAGVMGHEIAHVAARHGTKTATKGEIMQWATIPLILL
                     GPGGWAGYGLYEGLNLAIPMSYLKFTRDAEREADFLGLQYMYKSGYDPNAFVSFFEKI
                     EAEERRRPGSIPKVFSTHPPTPDRVQKAQEEITTILPARDEYIVTTSEFDTVKARLRR
                     IENKNKLDDKKNAGDKPTLRTRTEQTPQDKTSGTQQAPQSGGDDSDPDRPVLKKRTDD
                     GSGTGSGSGSGGSGSGSSTGTGSGSGPGTN"
     gene            5532..5759
                     /locus_tag="VG272_01310"
     CDS             5532..5759
                     /locus_tag="VG272_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12346.1"
                     /translation="MATAEQQIRVGQVWKKVGTAETFLVTKLYTEALTTIAVLRPTGA
                     ATESLIRVRVERRPDGHVLPGFSMAQENERP"
     gene            complement(5796..6515)
                     /gene="bshB1"
                     /locus_tag="VG272_01315"
     CDS             complement(5796..6515)
                     /gene="bshB1"
                     /locus_tag="VG272_01315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HWF12347.1"
                     /translation="MKLDLLAIAAHPDDVELTCGGTLLKATDQGYKTGILDLTAGEMG
                     TRGTPETRLKESAKAAKILRVAHRENLRLPDANLEVTKETKLAVARRIRALKPHTVIL
                     PYWEGRHPDHYTAARLAYEGCFLAGLKQLPIEGEAFRPFKILYSTVYYREVRPTFVVD
                     ISRYFDRRYDAILAYHSQFNPKAKAKKSKVDIPLDRLHHEVNLIARYYGQMAGVEYAE
                     PFLVKEVMQVEDVVKLPVRSV"
     gene            complement(6621..7304)
                     /locus_tag="VG272_01320"
     CDS             complement(6621..7304)
                     /locus_tag="VG272_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12348.1"
                     /translation="MKVGLFGATGMVGQGVLRECLLDPQIDTVVTIGRVAPRETDPKL
                     RNIVQPDLFHYSSIEPQLAGLDACFFCLGVSSAGLAEEQYEKLTYNLTMAAAETLSRL
                     NPGITFIYVSGAGTDSTEKGRVMWARVKGRTENALMRMPFKGAYMFRPGIIQPVHGEV
                     SKTKAYRILYFVFKPLFPLILRLFPRQVLTTEQIGRAMINVARHGAPKKILEISDIAA
                     CAGPRSPAY"
     gene            7640..8026
                     /locus_tag="VG272_01325"
     CDS             7640..8026
                     /locus_tag="VG272_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12349.1"
                     /translation="MTDFRAKRAPSIPGRGIEPIANARLSPFTGREKRRGERLNSRVP
                     IALEWNCAGQVQRKETQTRVVGPYGCMVVLPSNIEVKQTIRLTNMVNKESNQGVIVWR
                     GMERAEGWELGIELINPQMGFWGLDL"
     gene            8023..8397
                     /locus_tag="VG272_01330"
     CDS             8023..8397
                     /locus_tag="VG272_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWF12350.1"
                     /translation="MNNTKEQSSKGAALLAGEEKRRSQRVIVRVPVTLLLSENGQAGK
                     INCHTVAVNIHGAMVVSPRALEENAKLDIVNERTSEKIGCRVTRAARESSEGFLIPLE
                     FTTPAPNFWQISFPPANWKALD"
BASE COUNT         1920 a         2398 c         2332 g         2021 t
ORIGIN      
        1 acttttccct cctcgatgag tttcaccatc tcggcccagg agtcttcgac cggcgtgccc
       61 atttcgtccg gccaatgaaa ttggtagagg tcgatgcgct caatgccgag gcggcgcagt
      121 gacgcctcac actcgcggcg aatcgtttcc ggcttcaata cgcgcttggc ctgctgcatc
      181 ggattgttcg gatcccaggt cagcccgcac ttggtaaaaa ttaggggacg ttcggaagag
      241 ggtaaattgc gcaggagttt tccgaccact tcttcggagt ggccgagtcc atagaccgcc
      301 gcggtgtcaa tccagttgac gccaagttcc agcgcgcgcc gcatcgtgac gatcgattcg
      361 tcgtcatcct gcggtcccca gctgaacgcc cagccgccgc caccgatggc ccatgcgcca
      421 aaacctacgg tggtgatttg caagccgctg gaaccaagtg gacgcttcgg caaattcatc
      481 tgtgcccccg gagaagtttc gtgacgcgaa ttgttggaga cctgtagccg gtttctccat
      541 tccagtgtaa acgcgaatcg accgatgttc cagcgaagcg gcgtatgttc gattgttccg
      601 ggcaattttg ccatttacgc aaagtgccca tccaaaattc ggccggcctc tatgaaagcg
      661 gttggtagcg gccgctctgt cccgcgcttc ctggacagaa cgggcactgc tcgcgcaagc
      721 ctgcaacgat tccaaaattt agcgcgctct cattttgcta tcgacatgca gggccactgc
      781 cgatagactt ccgttcataa tcgctccatg aaaagttaac tttgtttttt tcctgatttc
      841 tctgtgcgct ctgtgtcgag tttttcgata gcgttagcac gcacagagaa atgaccagag
      901 aagcagagag ttcgtagcca aggccgcgag ccggtggcgt aaagggagtc acgatgcgaa
      961 atcgcaggga tttcattatg agcgtggtgg gtgcctccgc cggagttgtg tttacgggct
     1021 gttcgatctg cgatgcgatg gccggcgcgc gtcctccgca ggttggcagc ggtaaaaagc
     1081 acaagccggt aatagtgggc aaccgtcgcg taaaaacagt ggatattcat tgccacgtca
     1141 gcgtccccga agtgactgac ctccttaagg gcacgaaaat tgagcgccga ctcggagccg
     1201 gtctcgcgat aggcggatac gacgacgcgg gactgactcc cgcgcgaatc cagacgatgg
     1261 acgaaatggg catcgacatt caagccgtca gcatcaattc tttttggtat tcagcggaga
     1321 gagaacttgt caccaaattg atcgatttac agaatcaaaa attagccgaa ttgtgcgcca
     1381 cgcatccaga ccgttttgtc gcgtttgcct cggtcgcgtt gcagtttccg gaactggccg
     1441 cagagcagct tgaagtggcc atgaaaaaat acggattgag gggcgtggga atcggtggca
     1501 gctgcgacgg caaggaattg tcttcgccgg aattcgatcc cttctgggcg aaagcggaag
     1561 agttgcaggc gctcgtcttc attcatcctc aggacagcgc caacgcgaca ggtattcgca
     1621 accgcgtgca gggaaatggc gtgctgagta atgtgatcgg caaccctttg gaaacgacca
     1681 tgtgcctttc ccatttaatt ctggaaggga cgctcgataa atttccaaat ctcaaaattt
     1741 gcgcggcgca cggcggcggt ttcctgccta gctatgccgg gcgcatcgat caaggctgcc
     1801 gcgtgttccc caaagaatgc gataaaacaa ccaaaatgaa gccttcggaa tatctgaaaa
     1861 agatttacgt ggatacgatt gttttcaatc aggaagcgtt gcggcacttg attgccgtgg
     1921 tcggcgcggg ccagatcggt ctgggcacgg actacccgtt tccgtgggat ttcgcgcccg
     1981 tcgactacgt gatgaatcag cccggcctga gcgacgcgga ccgcatcgct atcctcggcg
     2041 gcacgatgag caagctcctg aaaatatcgt aactttcagt aggataggct tcagcctgtc
     2101 tgcttttgat ttttttagtt gcaacaaagc ccaaaagaag acaggctgaa gcctgtccta
     2161 cttgagccca tcccaatacg cttccagatt tttgacgacc gatttatcca gcatgggccc
     2221 atctcccgcg cgacaatttt ccgccatgtg cgccggatta ccggtacctg gaatcgcgca
     2281 agtcaccgct ttttggctca gcgtaaattt caataaaagt tcagaccacg agtcgcatcc
     2341 ggcctcgcga gcccatgccg gcaacgcttt tccgcgtagc ccgcgatgca gactgccctg
     2401 tccgaatggc cgatttgcga ggatcgcaat gccgcgatca agtgcgaggg gcaacaggcg
     2461 ctgctccgcg tcccgctcat ccaaagaata attgagctgc acgaaatcga gcgtttcgtc
     2521 gcgcataatt ttttcgagat ccgcatacgc gctggcgttg taatgtgtga tgccgagata
     2581 ccggatctgg ccctcctgtt tccacgcccg caacgttgcc agatgcgtgc gccaatcgag
     2641 cagattgtga atctgcatca ggtcgacctg cgtacatcgc aacaatttca tcgacttctt
     2701 catctgctca attcccgcgc tgcgtccttc gatccatacc ttggtggcta cgaacgtttt
     2761 tccccgcaca ccttgttcgg caatcagatc tccggcaact tcttccgcgc ggccatacat
     2821 aggagagctg tcgaccacgc tgccgcccgc cgaataaagt gtccgcagga cttcgccgac
     2881 ttcggcacgt gcatcgcccc cggagccgac gtcgaaacct ttgtatgtgc cgattcctat
     2941 aattgggagt tgctcgccag tggaaggaat ggttcgctta agcataccaa ccaatataga
     3001 cacgccattt attttttaca aatggactga aaacgccgac cacgggcatt cccgcggcgc
     3061 atatggaggt tcgtgtcgtg gccaagaccc gcccactccg tcctgaaggg cgcgggacgg
     3121 agcggcctct acgaaacaag cgcgcatcct acggttttcg tagcggccgg cagttttggc
     3181 ggacgggttt tatgtgcgtg caccgcaaat ccgcccacca ccacccgcga accttccttg
     3241 acacttcttc gcataggaca gtaaacttct tctccgaatt cttcagagga ccttaacgat
     3301 gctgctgtcg cgcgattacg tcggatacat ggccggagaa gtggtaaagc gcctacttgc
     3361 cgccaaggtg attgagacgc cggatgcggc ctccgttacc gggcgcgtgc gcgcggccat
     3421 gcaagacgaa atcagcattg aagaccgcct gaatgaagaa gtgcgcgaaa ttctgaccaa
     3481 gcacgccgat gaaatgcgcc gcacaggcgt gcaataccag gaaatgtata aaaaagtgaa
     3541 gggcgaactc gcccggcaaa gaaagctgat cctgcgttga tccaggctga gccagcaaga
     3601 ggaaagggaa gttcaacaac tcactgatgc gactgacccg cgaaaaatcc attcgccttt
     3661 cccacgtgat taccgatgtc ctggtcgcct gcgacaacgt ggaattcatc gacgatcgcg
     3721 acacgatccg ccagaaggca ttgcaaatcc tgcaggattt gctgaaggaa gaagaagcgg
     3781 tggatgcgga agtccgcaaa aaaatcagtt cgcagaaaaa agaaattcta gaagccagtg
     3841 aagagtggga cgtgctgtat cgcaagtatt atgcggatga aatgcgccgc atgggaatcg
     3901 ttcacctgcc gagcacgtcg taatttccca gttaaactaa tttagtagcg tttcaaatgg
     3961 cgcgggcttc agcctgcgag ttttagtcct tgcaaggacc aaacccggca ggctgaagcc
     4021 cgcgcacttg tgccgatacg acgagccaca tggtgctcaa tcttaattgg tgccaggtcc
     4081 tgagccggaa ccagtgccgg tgctggaacc cgagcccgat ccgcccgatc cacttcccga
     4141 acccgtgccg cttccatcat ccgtgcgctt cttcagcacc ggacgatccg gatccgaatc
     4201 gtcgcctccc gattgtggag cttgctgcgt tccgctggtt ttgtcctgcg gagtttgctc
     4261 ggtgcgcgtg cggagcgtcg gtttgtcgcc cgcatttttc ttgtcatcga gcttgttctt
     4321 gttctcaatc ctgcgcaggc gcgctttcac cgtgtcaaat tccgatgtcg tcacaatgta
     4381 ttcatcccgc gcggggagaa tcgtggtgat ttcctcttgc gccttttgca cgcggtccgg
     4441 agtgggcgga tgcgttgaga aaaccttggg aatgctgccg ggcctccgcc gctcttctgc
     4501 ttcgattttt tcaaagaagc tcacgaaagc attgggatcg tatcccgatt tgtacatgta
     4561 ctgcagtcca agaaagtcgg cttcgcgctc agcgtcacgt gtaaatttca gatagctcat
     4621 cggaatggcg aggttcaatc cctcgtacaa gccgtatccc gcccatccgc ctgggcccag
     4681 cagaatcagc ggaatcgtcg cccactgcat gatctcgcct ttggtggcag tttttgtgcc
     4741 gtggcgcgcg gcaacgtgcg caatctcatg gcccatcacg cctgcaagtt cagcctcttc
     4801 gtccgcgcgc aggatcaagc cgctatttac gtagaaaaat ccgccgggaa gcgcgaacgc
     4861 attaatttcg tccgagtcga tcactttgat ggtgaaaggg acttttgcgt ccgaattgcg
     4921 caccaggttt tggcccacgc gattcacata ctcggtgacc acgggatcgt taatcatgcg
     4981 cgcggttttt ccgacttcca tcgcggcctg ttttccaagg gcgatttcgc gctgcaggga
     5041 atacaaattt acgccgttgc ctacgcctcg attgccgatt gcattcacgt ctttatcgga
     5101 acctggcttc acgcctttcg ccgtggcgtc ttcatcagaa gcgggaggcg tcgcctcttc
     5161 ctgcgtagga ggcgtttgag gaggatccgt cgttgtgggc gtttgggttt gggtctgagg
     5221 ttgcggttga ctttggggtt gctgcgcctg accaggcgct gcgtaacccg tcggcgcacc
     5281 aaccaccgcc gcaaaaataa tcgcgatcag cccgcttgag aggcgtctca tagctcctcc
     5341 tgaaactcta accctagtag tatacattac gcgcttgttc cttgagagtc cacctgcgcg
     5401 gtgagatgtg cgtccgtgca aataggtttc ccgcgttcct tccttctatt gagatatgtc
     5461 tctgatatgg tgtggaatac ctgacctttc cagtaattga cacccggcag agttaactct
     5521 aacctcagaa tatggcgact gcggaacagc agatacgcgt ggggcaagtc tggaagaaag
     5581 tcggcaccgc cgaaactttc ctcgtgacca agctctacac tgaggcgttg accacgatcg
     5641 cggttttgcg gcccacaggc gcggcgactg agtccttgat tcgcgtccgt gtcgagcggc
     5701 gcccggacgg gcatgtcctt ccgggattct ccatggccca ggaaaacgag cgaccataac
     5761 agctttgcgt ttggacgcca acccgcttca gtacttcaca cggaacgcac aggcagcttt
     5821 acgacgtcct ccacctgcat cacttccttt accaagaacg gctcggcata ttccacgccc
     5881 gccatctgcc cgtaataccg cgcaatcaaa tttacttcgt ggtgcaaacg gtccagagga
     5941 atatccactt tcgatttttt agctttggct ttgggattaa attgcgagtg gtaagccaga
     6001 atcgcgtcgt atcgccggtc aaaatagcgc gaaatatcca ccacaaacgt ggggcgaacc
     6061 tcgcggtaat acacagtgga atacagaatt ttaaacgggc gaaacgcttc gccctcgatg
     6121 ggcagctgtt ttaatccagc caaaaagcag ccttcgtacg caagacgcgc ggcggtgtag
     6181 tgatccgggt gccgtccctc ccagtagggc agaatgacgg tgtgaggttt cagcgcgcga
     6241 atgcgccgcg cgacggccag tttcgtttcc ttcgtgactt ctagattcgc atccggcaag
     6301 cgcaaatttt cacgatgcgc cacgcgcaga attttcgcgg ctttcgcgga ctcttttaag
     6361 cgcgtttccg gggttccacg cgtgcccatt tcgcccgccg tcaaatccag aatgcccgtt
     6421 ttgtagccct gatccgtcgc tttcagcaat gtgccgccac acgtcagttc cacatcgtcg
     6481 ggatgcgccg caattgccag aagatccagt ttcatcgctc ctccagagac gttcgggaaa
     6541 acaccacagc ctagcatatt gtgaatgatt cattcgtgag cggagcgcgc cgttgcagca
     6601 atcagcaccg taaccttgct tcagtacgcg ggcgagcgtg gccctgcgca cgcggcaatg
     6661 tcgctaatct ccagaatttt cttcggtgca ccgtgacgcg cgacgttaat catggcacgt
     6721 ccaatttgtt ccgtggttaa aacctggcgc ggaaaaagcc gcagtattag cggaaaaagc
     6781 ggtttaaaca caaaataaag gatccggtac gcctttgttt tggaaacctc tccgtgcacg
     6841 ggttgaatga ttccgggtcg aaacatatac gcgcctttga acggcatgcg catcagcgca
     6901 ttttccgtcc taccttttac gcgtgcccac atcacccggc ctttttccgt actatccgtt
     6961 ccggccccgg aaacgtagat gaaagtgatt cccggattca gccgggacag cgtttctgcg
     7021 gcggccatcg tgaggttata agtcagtttt tcgtattgct cttcagccaa cccggccgag
     7081 gaaacgccca gacagaaaaa gcatgcatcc aggccagcga gttgaggttc gattgatgag
     7141 tagtgaaaaa gatcgggctg aacgatattg cgaagcttag gatccgtttc cctcggcgcg
     7201 acgcgaccga tggttacgac ggtatctatc tgagggtcga gaaggcattc gcgcaaaaca
     7261 ccttgcccga ccatgcctgt cgccccaaat aagcctactt tcattgccgc agccgccttt
     7321 cgttccagta ggacaggctt cagcctgtca gcttttgaac tttgaacact ccgccacaag
     7381 ccattggcgc agcgccacaa gtcaaaagaa gacaggctga agcctgtcct actaaatcac
     7441 gtccagctca ttatatttta ttaatttcac gattttccgc gcatttcgca cgtttcgtaa
     7501 cctgtccttt ccctaaaatc tccctgtccc cgcgtaccat tgacgctatt ccggcacgac
     7561 actagcatta tcactctaag gtttttgtcg atattagtga tagtaaacag cttcttgcag
     7621 cccactgagg gggcttggaa tgaccgattt tcgcgccaag cgggcaccca gcataccggg
     7681 ccgaggcatt gagcctattg caaatgccag gctgtcccca ttcaccgggc gcgagaagcg
     7741 gcgtggcgag cgccttaact ctcgtgttcc cattgcgttg gagtggaatt gcgccggaca
     7801 ggttcagcgc aaagagacgc agacccgggt tgtgggtccg tatggatgca tggtagtcct
     7861 tccgtcgaat atcgaagtca agcagacgat ccgactgacg aatatggtga acaaggaatc
     7921 gaatcagggc gtgatcgtat ggcggggcat ggagcgcgcg gaaggctggg aattgggaat
     7981 tgaattgatc aacccacaaa tgggattttg gggactggat ctgtgaacaa tacaaaagag
     8041 cagtcaagca aaggtgccgc acttctggcg ggagaagaaa agcgcaggag tcaacgcgtg
     8101 attgtgcgcg tgcctgtaac gcttttgttg tcggaaaacg gccaggcggg aaagatcaat
     8161 tgccataccg tggccgtgaa tatccatggc gcgatggtcg taagtccgcg cgcgcttgaa
     8221 gaaaatgcga agctggatat cgtgaatgaa cgcacctcag agaaaatcgg ctgccgtgtg
     8281 acacgcgcgg ctcgagaaag ttcggaagga tttctgattc cattggaatt caccacgcct
     8341 gcgccgaatt tctggcaaat ttcgtttccg cctgcaaact ggaaagcgct cgactagtcc
     8401 ggcacactta gattgcacaa atgcaaatgg atgggatttc gttttggcga ccacgcccat
     8461 ctttgtcctt ttgtttcaat attttcgccg tttttcgctg ggaaaaccct tctgtgaatt
     8521 ccgcatcaaa caatttgaca aacttacagg ttcgccagct aatggtttaa tgcagtgtag
     8581 tcttggggtt tgcggggcct tgctcgattt tcattttgtt ttgcttagag gaaaacgtga
     8641 ataacgcttt cagtgtgtat gtgtctgccg g
//
LOCUS       DASZMB010000032         5565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3330510, whole genome shotgun sequence.
ACCESSION   DASZMB010000032 DASZMB010000000
VERSION     DASZMB010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3330510"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VG272_01335"
     CDS             <1..329
                     /locus_tag="VG272_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12351.1"
                     /translation="AGLEGGAGAQLAQAAGDVCREAISQGGGNTGIEVTLDTFQDRVE
                     VAIHYRGQGLLTDGTGGLVVTGTKSKGAESLNGEDLLSRVDRVNCKADEGIARVTLVK
                     YLPAHH"
     gene            complement(461..979)
                     /locus_tag="VG272_01340"
     CDS             complement(461..979)
                     /locus_tag="VG272_01340"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWF12352.1"
                     /translation="MKHKILGPLALILAAAVLYAFAAPSYRQGEPSLAGRKAQDFDMQ
                     ITGASHLSDLRGKVVVLNFWASWCPPCLEETQSLNTLQKAIAPKGAVVLGISVDEDKD
                     AYEKFLADNHVVFPTYRDATKKTATDYGTSMFPETYLIDREGRLARKIVGPQDWQSPE
                     IMQSIDILLKQN"
     gene            1066..2082
                     /locus_tag="VG272_01345"
     CDS             1066..2082
                     /locus_tag="VG272_01345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWF12353.1"
                     /translation="MNSNSTVSAIVPARNEEATIAAAVESLASQPEIGEIIVINDQST
                     DGTVAILQQLSARFPELHILETRELPSGWVGKNYAVSLGAVEAKGEWLLFTDADGVHL
                     PGSTAHALADAGEFGAGLVSYSPEQETRFWWEKALIPFVYLRLSHRFRYAEVNHPESP
                     EAAASGQYLLIRREDYLRIGGHAAVAAEVLEDVALARVAKNAGVRLHFASGSGIIRVR
                     MYRTFRSMWEGWTKNLYPLIGGTSRTVGRELFRVVPWIPLLLLPLGLVHLMWGVLGVA
                     LLAGRHAAYAAELRRNRFPGRLALYYLPGVALYSMVLLNSEWRYGHGSVGWKGREYPV
                     TRDL"
     gene            2072..2584
                     /locus_tag="VG272_01350"
     CDS             2072..2584
                     /locus_tag="VG272_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HWF12354.1"
                     /translation="MICNSQEPASQHHLDYVTISKPFMVYLTRKLEFSASHLYHNPAF
                     SAEENRRVFGKCNNPHGHGHNYVLEVTVAGEPNPATGMVLDLKELKEILQREISDRMD
                     HRFLNYEVPELSGQIPTCENIATVIWRLLEPKIASGHLHRVRLYETPDLFVDCYGEGD
                     GAHSNVGDAR"
     gene            2581..2988
                     /locus_tag="VG272_01355"
     CDS             2581..2988
                     /locus_tag="VG272_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HWF12355.1"
                     /translation="MKLQLWRRYKFCASHRLHSAQLGEEENRRVYGKCNNPHGHGHNY
                     VVEVAVSGPVDPATGMIANLTELDAFVEKEILEPFDHKYLNEEIAEFQENIPTTENIC
                     IEIFNRLRRFSRAKLERVRVEETGLNSFEYAGE"
     gene            3002..3619
                     /gene="folE"
                     /locus_tag="VG272_01360"
     CDS             3002..3619
                     /gene="folE"
                     /locus_tag="VG272_01360"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HWF12356.1"
                     /translation="MSDDRKQVEELMLNDAGSAETKVTVADSVRKILVEIGEDPNRDG
                     LRRTPERFEKAFRFLTSGYHQDPEKLLNGAMFTVCYDQMVLLKDIEFYSLCEHHLLPF
                     FGKCHVAYIPDKKVVGLSKIPRLVNMFARRLQIQERLTNQIANSIQQKIAPLGVGVVI
                     EGRHLCMAMRGVEKESSQTVTSAMLGSFKDNPKTREEFLSLIHGR"
     gene            3858..4118
                     /gene="rpsO"
                     /locus_tag="VG272_01365"
     CDS             3858..4118
                     /gene="rpsO"
                     /locus_tag="VG272_01365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HWF12357.1"
                     /translation="MREAKSMLIEQYRRAVKDTGSPEVQIALLSERINYLTSHLKTHL
                     KDHASRRGLIMMVNKRRRLLDYLNRRNPDRYREIVERLSLRK"
     gene            4372..>5565
                     /locus_tag="VG272_01370"
     CDS             4372..>5565
                     /locus_tag="VG272_01370"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWF12358.1"
                     /translation="MSQAAPAPHQVTVEVGGRTLTLETGKIAKQANGAVLARYGDTVV
                     LVTACMDSKPNDRDFLPLTVDYREYTYSAGKIPGGFFKREGRPSEREILTSRMIDRPL
                     RPLFPEAWRNETQIVAMVLSADSENDPDMIGITAASAAAFISDLPFTTPVAAVRVGLV
                     DGKLIANPTTAAQKTSQLNIVVVATEQAIVMIEAGAMEVSEDTVAGALEFAHEQIKLI
                     ITAIRQLHDKLKPNKVTVAPLPFDEALAKEIEQKYGTQLHDAIDTKKHPKKESYHLID
                     EVKKAVKESIPEENEEKRTLAMRAFERLRENYFREDILQRNRRPDARAFDEIRDITCE
                     VGVLPRAHGSALFTRGETQALATTTLGTKEDKQRLDLLFEEESFKRFMLHYNFPAFSV
                     GEVKFL"
BASE COUNT         1326 a         1340 c         1661 g         1238 t
ORIGIN      
        1 atgcgggact tgagggcggg gcgggagcgc aattagcaca ggctgccggg gatgtctgcc
       61 gcgaggcgat ttcgcaaggc ggcgggaata caggcattga ggtgaccctg gatacgtttc
      121 aggaccgcgt ggaggtcgct atccattaca gagggcaggg attgctcacg gatgggaccg
      181 gaggcctcgt ggtcactggg acgaagtcca agggcgccga aagcttgaat ggagaggatt
      241 tgctttcacg cgtcgaccgt gtcaattgta aggcggatga ggggatcgca cgcgttacgc
      301 tagtaaaata tttgcctgca catcactgat attcatctct tcttactctt cctattccca
      361 attacagaac taatatatgt ggcacaggcg ttagcctgtg gtgtggaagt taggcttgca
      421 tggactaaac cccacaggct gaagcctatg ccacggaatg ctagttttgt tttagcagga
      481 tatcgatgga ctgcatgatt tcaggggatt gccagtcctg gggacctacg atcttgcgcg
      541 ccaggcggcc ttcgcggtcg atcaggtaag tttccgggaa catggatgtg ccgtaatcgg
      601 tagccgtttt cttggtggcg tcgcgatagg tggggaagac gacgtgatta tcggccagga
      661 atttttcgta ggcgtcttta tcttcatcaa cgctaatccc taaaacgact gcgccctttg
      721 gtgcgatggc cttctgcagg gtattgagcg attgcgtttc ttcgaggcac ggcggacacc
      781 acgacgccca gaaattcagc acgaccactt ttccacggag gtccgacaga tgcgacgcgc
      841 ctgtgatctg catgtcaaaa tcctgagctt tgcggcccgc gaggctcggt tcaccctggc
      901 ggtaggacgg cgcggcaaag gcatagagca cagccgcagc aaggatcaac gcaagaggac
      961 ctagaatttt atgtttcaat atttgttctc cagcccttct ataatacatt catcagcgcg
     1021 tttcgtgcga accgaattgc cgcatactga gtaagcgatt aaacaatgaa ttccaattcc
     1081 acggtttcgg ccattgtgcc cgcacgcaac gaggaggcga cgattgccgc agccgttgag
     1141 tcgctcgcgt cgcagcctga aattggagaa attattgtca tcaacgatca gtcgacggat
     1201 ggcacggtgg ccatattgca gcagctttcc gcgcgattcc ctgagctgca cattttggaa
     1261 acgagggaat tgccgagcgg atgggtgggc aagaactacg cggtgtcgct cggcgctgtg
     1321 gaggcgaagg gcgaatggct gctctttaca gatgcagacg gcgttcattt gcccggttcc
     1381 acggcgcatg ctttggcgga tgctggggag tttggggcag ggttggtttc gtattcgccg
     1441 gaacaggaaa cgcgcttttg gtgggagaaa gctttaattc ctttcgttta tctgcgattg
     1501 agccacaggt ttcgatacgc ggaagtgaac catcctgaat caccagaagc ggctgccagt
     1561 gggcaatact tgctgatcag gcgagaggat tatctgcgaa tcggcggaca cgcggcagtg
     1621 gcggcggaag tgcttgaaga tgtagcgctg gcgcgagtcg cgaaaaatgc tggcgtgagg
     1681 ctgcactttg cgtcaggaag tggaatcatc cgtgtccgga tgtacagaac atttcgctcc
     1741 atgtgggaag gctggacgaa gaacttatat ccgctcatag gagggacgtc gcgcacggta
     1801 gggcgggagc ttttcagggt ggtgccgtgg atacctttgc tgctacttcc tttggggcta
     1861 gtgcatctaa tgtggggcgt gctgggggtt gcccttctgg ctggacgtca tgccgcatat
     1921 gcagccgaat tgcggcggaa tcgttttcca ggaaggcttg ctctatacta tttgcccggg
     1981 gtggcgctgt attcaatggt tctgttgaac tcggaatggc gttacgggca tggcagcgtg
     2041 ggttggaagg ggcgcgagta tcccgtgact cgtgatttgt aattcgcaag aaccggcttc
     2101 tcagcatcac ctggattacg taacgatttc aaagccattt atggtctatc ttactcgcaa
     2161 gcttgaattt tcggcgtcgc atctttatca caacccggca ttttcagccg aggaaaaccg
     2221 gcgcgtcttt gggaagtgca ataacccgca tggtcacggg cataattacg tgttggaagt
     2281 aacggtggcg ggcgaaccga atccggcgac gggtatggtg ctggacctga aagaattgaa
     2341 agaaattttg cagcgcgaaa tttcggacag gatggatcac cggttcctga attacgaagt
     2401 gccggaattg tccggacaaa ttcccacgtg cgaaaacatt gcgacggtga tctggcgtct
     2461 actggaaccg aaaattgcga gcgggcattt gcaccgggtg cgcttgtatg agacgccgga
     2521 cttattcgtc gattgctacg gcgaaggcga tggcgcgcat tcaaatgtgg gagacgcgcg
     2581 atgaagttgc aactgtggcg gcgctataag ttttgcgcgt cgcaccgatt gcacagtgcg
     2641 caactgggag aagaagaaaa tcgccgcgtt tacgggaagt gcaacaaccc gcacgggcat
     2701 ggccataact atgttgtgga agtggcggtt tccgggccgg tcgatccggc aaccgggatg
     2761 atcgcgaatt tgacggaact ggatgcattt gtggaaaaag aaattctcga accgttcgac
     2821 cataaatatc tgaacgaaga aattgcggag tttcaggaaa atattccgac gacggaaaat
     2881 atttgcatcg agatttttaa tcgtctgcgc cgcttttcgc gagcgaagct ggagcgtgtg
     2941 cgggtcgagg aaaccggact gaatagtttt gaatacgcgg gagagtaaac aggagtgacg
     3001 catgagcgat gatcgcaagc aagtggaaga attgatgctg aacgatgccg gcagcgcgga
     3061 aacaaaagtg accgtcgcgg attccgtgcg aaaaatttta gtggaaattg gcgaggatcc
     3121 gaatcgagac gggttgaggc gcacgccgga gcgctttgaa aaagcgtttc gatttttgac
     3181 cagtggttac caccaggacc cggaaaaatt gctgaacggc gccatgttta cggtgtgcta
     3241 tgaccagatg gtgctgttga aggacattga gttttacagt ttgtgtgagc atcacctgtt
     3301 gccgtttttc gggaaatgcc acgtggcgta tattccggac aagaaagttg tgggcctgtc
     3361 gaaaattccg cgactcgtca atatgttcgc gcggcggtta cagattcagg agcggctgac
     3421 aaatcagatt gccaactcga tccagcagaa gatagcgccg ctgggtgtgg gcgtggtaat
     3481 cgagggtcgg cacctgtgca tggccatgcg cggggtagaa aaggaaagtt cacagacggt
     3541 gaccagcgcg atgctgggct cgtttaagga caatcccaag acgcgcgagg aatttttgtc
     3601 cctgatacac ggcaggtagg gaagcctcta gaggatctaa agcgccgacc ttatagattt
     3661 ggatagattg gcattttcgg ggtcaacaca aactgaccag atgcccgcct gaaggcgggc
     3721 gctacaaaag acaggcctta aatcgtgctt gcgagaagaa aatttttgca tgtcatacag
     3781 tccctatggt agtctgctag ggtcgaagag aagacaaaac gagagctaaa tatagtgggt
     3841 aaggcggaga gggtattttg agagaagcga agtcaatgtt gatcgagcag tatcgacgtg
     3901 cagtaaagga tacgggctca ccggaagtac aaattgcttt attgagcgag cgaatcaact
     3961 atctgacgtc gcatctgaag acgcacctca aggaccatgc atcgcgacgc gggctgatca
     4021 tgatggtcaa caagcgccgc cgtctgttgg attatctcaa ccgtcgtaac ccggatcggt
     4081 atcgcgagat tgtcgaacgc cttagtttgc gcaagtaaac gctcctcagg gggagtgcag
     4141 tcgcgccaac ttcatctacc gacgttcgcg gagttcggta agcttctggc ggccccaggc
     4201 atcgttgccc ggccgctttt tttgtaggca cactccgaaa cgtcactcgt agccgttctc
     4261 tgttttctct tccaaacaaa cgggaaaagt ttctcccgca ctcgaatggc tttccgcacg
     4321 cgtgtggccc gatggttcgg ggcgcggcgg gagtcgaaag gaccacttca tatgtcgcaa
     4381 gcagcacccg ctccccatca ggtgacagtc gaagttggcg gacgtacgct gacactggaa
     4441 accggaaaaa ttgcaaagca agccaatggc gccgtactgg cgcgatacgg cgacaccgtc
     4501 gtgctggtca cggcgtgcat ggactccaaa ccgaatgacc gggattttct tccgctcacg
     4561 gtggactatc gcgaatatac gtattcagcc ggaaaaattc ccggcgggtt ttttaaacgc
     4621 gaagggcgcc cttcggagcg cgagattctg acttcgcgca tgatcgatcg tccgctgcgc
     4681 cccttgttcc cggaagcctg gcgcaatgaa acacaaatcg tagccatggt gctttccgcg
     4741 gatagcgaga acgatccgga catgatcggc attacagccg cttccgctgc cgcgtttata
     4801 tcggacctgc cgttcacgac cccagtcgcc gcggttcgcg ttggcctggt ggatggaaaa
     4861 ctaattgcta atcccacgac agctgcgcag aagaccagcc agttgaatat cgtcgtggtg
     4921 gcaaccgaac aagccatcgt gatgatcgaa gcaggagcga tggaagtcag tgaagataca
     4981 gtcgccggag cgctggaatt cgcacatgaa cagatcaagt tgatcatcac cgcgattcgc
     5041 caactgcatg acaagctcaa gcccaacaag gttaccgttg cgccgctgcc ttttgacgaa
     5101 gcgctagcca aggaaatcga acagaaatac ggcacgcaac ttcatgacgc aatcgatacc
     5161 aagaagcacc cgaagaagga aagctatcac ttgatcgacg aagtcaagaa agcggtaaaa
     5221 gagtcgattc cagaagagaa tgaagagaag cgtacgctgg ccatgcgtgc atttgaacgg
     5281 ttgcgcgaaa attatttccg cgaagatatt ttgcagcgta accggcgtcc cgatgctcgc
     5341 gcattcgacg agatccgcga catcacctgc gaagtgggag tgttaccccg agcgcacggc
     5401 tcggcgctct ttacgcgcgg cgaaacgcaa gctctggcta ccacgacgtt gggaaccaag
     5461 gaagacaagc agcggctcga cctgctcttc gaggaagaat cattcaagcg cttcatgctg
     5521 cactacaact tcccggcatt ttccgtcggc gaagtgaaat tcttg
//
LOCUS       DASZMB010000033         7039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1674215, whole genome shotgun sequence.
ACCESSION   DASZMB010000033 DASZMB010000000
VERSION     DASZMB010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7039
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1674215"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VG272_01375"
     CDS             complement(<1..491)
                     /locus_tag="VG272_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWF12359.1"
                     /translation="MEPIVVTENLWKLYRAGKVEVPALRGVNFKVMPGEFVSVMGPSG
                     CGKSSLLYVLGGLATASKGRVLVDGNDLSTMSDADRTKLRRDKIGFVFQRFNLLPTLD
                     AQGNIAIAQHIHGNGFNSGRFETITKLLGLTERLKHRPSEMSGGEQQRVAIARAIIND
                     PKI"
     gene            complement(655..1755)
                     /locus_tag="VG272_01380"
     CDS             complement(655..1755)
                     /locus_tag="VG272_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF12360.1"
                     /translation="MIRQLVVNNVLHRPIRTIVSVLAVGVEVALVILIVGLTSGLLQE
                     TAKRIEGIGADIMLQPPSASVFLAFSGAPMPIKIADKLRELKDVRAVAPVLLQFSSSN
                     GVDIIYGIDPKSFRDVSGGFVFLEGHDMQGPDDILMDDWAAKAKNVAVNSTFRLLDHD
                     FHVAGIVEHGKGARLFVPLSTLQDLSGSRDKASVFFMKCTDPNLTQSVMDEMKPIFPG
                     YEIRPLKDFLSLMSATNLPGLNAFIESMIVLAVSIGFLVILLSMYTTVIERTRDIGVL
                     KSIGASKGYIIRALLGESVFICVIGVGVGIAMSYITRAIFLSSFPTLSILITPDWIVR
                     SAVIALAGGILGASYPAWLASRKDAIEALSYD"
     gene            complement(1752..2612)
                     /locus_tag="VG272_01385"
     CDS             complement(1752..2612)
                     /locus_tag="VG272_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12361.1"
                     /translation="MQAAGCITIKRRTTFKKEEILPELESNEEDLLASYNKQVKSVQT
                     IQATVDLVPTTGTTYSGVVEEYHDVPGFILAERPATVRIIGQAPVVAKNIFDMVADEK
                     EFRIYIPSKNSFLVGPTALIRPSKKPLENLRPQHVVEALFWPELRSTDNVLFEQFDFN
                     VSRYYILSLLRQAENGKLSIARKIWYNRTDLRPSRVQIFGVGGKLDSDITYSDWQPSP
                     SAPGSAATDETIFARDIHIWRPQDDYKLEIKILKVTLNETIAPDRFDLTQPPGTDLIH
                     VGDEISKVQR"
     gene            2966..4165
                     /locus_tag="VG272_01390"
     CDS             2966..4165
                     /locus_tag="VG272_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWF12362.1"
                     /translation="MSSFTETGLVTRTADRSGDVELSVVMPCLDEAATVGICVKKAME
                     ALNRHGIRGEVIVADNGSTDGSQQIAQEMGARVVPVERRGYGSALQSGIAAAHGEFVL
                     MGDADDTYNFAQLDEFVAKLREGYDLVMGNRFKGKILPGAMPPLHRYLGNPVLTGLGR
                     LFFKSPVGDFHCGLRAFRRDAIERLGLRTFGMEFASEMIVKAAAFGLRVTEVPTTLAP
                     DRRDRPPHLRTWRDGWRHLRFLLLYSPRWLFLYPGFALFALGIAVSAFLLPGPLRIGS
                     VVFDVHTLLFAAMAILIGFQSVIFATFTKVFAISEGLLPEDPRLTRMFRYVTLEVGLI
                     LGILLILAGAGAWILGLEYWRIREFGALDPEKTLRIVIPGVVCFTLGFQVVLSSFFLS
                     VLGMSRR"
     gene            4162..4860
                     /locus_tag="VG272_01395"
     CDS             4162..4860
                     /locus_tag="VG272_01395"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWF12363.1"
                     /translation="MKETEDLFDHYAAAYDEALSNAIAPSGESREYFAEGRVAWLKHC
                     LYELKLPVRSILDFGCGDGATTPLLFQALHAQSADGVDVSAKSLEIARKQHGTEQIHF
                     ESIGDFQPSGQIDLAYCNGVFHHILPEQRAQALSMVHGALRPGGLFAFWENNPWSLAT
                     RYVMSRCAFDRDAITLTPPEARKLLRQGGFEILRTDFRFIFPRSLRALRKMEDWICRA
                     PLGTQYQILCRKSS"
     gene            complement(4886..6091)
                     /locus_tag="VG272_01400"
     CDS             complement(4886..6091)
                     /locus_tag="VG272_01400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF12364.1"
                     /translation="MRLLQVTQSYYPFLAMGGPTVKVRALAKGLASSGNNVTVLSVDL
                     GLDQQKHNEIGAIPTRWGFEANENGVGAIYLRTRARYRALTWNPGVCAFCRERLSGFN
                     LAHIYGLYDFLGPQVASECRRIGRPYIVEPMGMFLPIVRNIRMKRLYHKMLGERLIRD
                     ASRVIATAEQERQEFLNGGIPDSKIVVRRNGIDVPTDFPAAGSFRDRWNIARGAKLIL
                     FLGRLVSKKSPDLMLEVFSRWIQRGNGARGSVLVMAGPDEGDGFREHLESLAVKMKLN
                     GQVLFTGPLYGDDKWAAYRDSDVFVLPSQNENFGNTAAEAIACGTPVLVTDRCGIAPM
                     VEQGAGLVVPHECDALEAGLARILEDRALALQLRRGCEDVKHSLSWAEPLAQMEALYQ
                     TIISENGGQ"
     gene            complement(6253..>7039)
                     /locus_tag="VG272_01405"
     CDS             complement(6253..>7039)
                     /locus_tag="VG272_01405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWF12365.1"
                     /translation="FTHGVEVWQPLGWTRGRALRSADLVLGPSRDTVKQLIELQQIPC
                     SRVRRLAWGLDPDFESRLASPASPTVPNGFPPNSRAILSVGRWNSAEKYKGADTLILA
                     LPRVLKAMPETSLVLVGDGDDRARLEQLAHDLGVSKSVQFLVGLTPDELFACYAHCEI
                     FALPSKGEGFGLVFLEAMAHGKPVVGGAHGGIPDIVEDGVTGLLVQHGDVDNLAQALE
                     SLLSNFDRATEMGVRGKDRVAQKFSFLEFEKQLTANLNRVLTR"
BASE COUNT         1528 a         1946 c         1962 g         1603 t
ORIGIN      
        1 ataatcttgg ggtcgttgat gatcgcacga gcgatagcca cgcgctgctg ctccccacca
       61 gacatttccg aaggcctatg cttcaggcgt tccgtgaggc cgagcaactt agtaatcgtc
      121 tcgaatctgc cggaattaaa accgttaccg tgaatgtgct gtgcgatggc gatgtttcct
      181 tgcgcgtcga gcgtgggcag cagattaaat ctctgaaaca caaaaccaat tttgtcgcgt
      241 cgcagcttgg ttctgtccgc gtcgctcatg gtgctcagat cgttgccgtc caccaggact
      301 cgccccttgg aagccgtcgc caatccgccc aagacataga gcaaagagga tttgccgcaa
      361 ccagacgggc ccatgacgct gacgaactct ccgggcatca ccttaaaatt aacgccgcgc
      421 aacgccggaa cttccacctt gccggcgcga tatagcttcc ataaattttc ggttacgact
      481 atcggttcca ctctaaggtc ccttgtggtg atcagcgcat cggcaggcgg acgtattgta
      541 atcgcgtttg acaattctta ctaggcccgt agccgcaata tgcaggctca aattatgacg
      601 attattgctg gagcgaaccc cagcggcgcg cgcgtttcag cgtgtcagcc agcatcagtc
      661 ataggaaagc gcttcgatgg catccttgcg gctagccagc cacgccggat aactggcgcc
      721 gagaattccg cccgcgagcg caataaccgc ggatcgcaca atccaatccg gcgtgatcaa
      781 aatggacaag gttggaaacg aggacagaaa aattgctcgc gtaatgtagc tcatggcaat
      841 gccgacgcca acgccgatta cgcagataaa aaccgactcg ccgagcagcg cgcgaataat
      901 gtagcccttg gacgcgccaa tggatttcag gacgccaatg tcccgcgtac gctcaatgac
      961 cgtggtgtac atcgacagca gaatgacgag gaaaccgatc gagacggcta gtacgatcat
     1021 gctttcgata aaggcgttca ggcccggcag attcgttgcg gacatcagcg aaagaaaatc
     1081 tttcaagggg cgaatttcat agccggggaa aatcggcttc atttcgtcca tcacggactg
     1141 cgtgagattc gggtcggtgc acttcatgaa aaaaacggag gctttatcgc gagacccgga
     1201 aaggtcctgc aacgtggaga gcggaacgaa caggcgcgcg cctttcccgt gttccacaat
     1261 tccggccaca tgaaaatcgt gatccaggag gcgaaaagtg ctgttcaccg cgacgttttt
     1321 tgctttcgcg gcccaatcgt ccatcagaat gtcgtcgggg ccctgcatgt catgcccttc
     1381 cagaaaaacg aaaccgccgg aaacatctcg gaaggacttg ggatcgatgc cgtaaatgat
     1441 gtcgacgcca ttcgaggaac tgaattgcag gaggacaggt gcaaccgccc gcacatcttt
     1501 tagttcgcgc agtttgtctg cgattttgat aggcatggga gcgccgctga acgccagaaa
     1561 aacagaggct gagggcggtt gcagcatgat gtccgcgccg atgccctcga tgcgcttggc
     1621 ggtttcctgc aagaggccgc tcgtgagtcc cacaatcagg atgacgagcg ccacttcgac
     1681 acccacggcg agaacactta cgatcgtgcg gatcgggcga tgcaggacgt tattcaccac
     1741 taactgacgg atcatcgctg taccttggaa atttcatctc ccacgtgaat caggtcggtg
     1801 cccggcggct gggtcaggtc aaagcgatcg ggcgcaatgg tttcgttcag cgtcaccttc
     1861 aaaatcttga tttccagttt gtaatcgtct tgcggacgcc aaatatggat gtcgcgtgca
     1921 aagatggtct cgtccgttgc cgcgctgccg ggcgcggaag gactgggctg ccagtccgaa
     1981 tacgtgatgt ctgagtcgag ttttccaccc acgccaaaaa tttgcacgcg cgatggacgc
     2041 aggtccgtgc ggttgtacca gatctttcgg gcaatggaca attttccgtt ttccgcctgt
     2101 cggagcaggc tgaggatgta ataacggctg acgttgaaat cgaattgttc gaataataca
     2161 ttgtcggtcg agcgcaattc gggccagaac aacgcctcca cgacgtgctg ggggcgcaag
     2221 ttctccagcg gctttttcga tgggcgaatc agggcagtgg gacccacgag aaatgagttt
     2281 ttggagggaa tataaatgcg aaattccttc tcgtcagcca ccatgtcaaa aatgtttttt
     2341 gcgactaccg gcgcctgtcc gataatgcgc acggttgcgg ggcgctcagc cagaataaat
     2401 cccgggacgt cgtggtattc ctcgaccacg ccgctataag tcgtgccagt ggtcggaacc
     2461 aagtccaccg tcgcctgaat ggtttgcacg gatttgacct gcttgttgta gcttgccagg
     2521 aggtcttcct cgttcgattc caattccgga agaatttctt ccttcttgaa cgtggttctt
     2581 cgcttaatgg tgatacaccc cgccgcttgc aaggcaaata tcgccagcag caggatccag
     2641 attctcggat ttttactcag tggttcttac tccctcagtg gaatcattca atctaacaac
     2701 cgctccggag ggtgtcaatt ttaaacgccg aagtgggttg ctcaattttc tacaaaaaaa
     2761 ctcgcagtag cctcacgttc ttaattttat cagtgtgcgg acatggtgcg gtataagttg
     2821 ttagcctgaa tcgtttaaag ccggctaatc gcaagccttt tccctgagta cgggatgggt
     2881 tagagttctg gagaccagta ggacaggctt cagcctgttc taccttagtt tggctaacgc
     2941 gcgcgacaga ggcgtattca atccaatgag ttctttcacg gagacgggtt tagtcacgcg
     3001 aactgccgac cgtagcggtg acgtggaact atccgtcgtg atgccctgcc tggacgaagc
     3061 ggccacggtg ggaatttgcg tcaaaaaagc aatggaggcg ctgaaccggc acggaatacg
     3121 cggcgaagtg attgtcgcgg ataacggcag cacggatgga tcgcagcaga ttgcgcaaga
     3181 gatgggcgcg cgcgttgtgc cggtcgagcg ccgtgggtat ggcagcgcgc tgcaaagcgg
     3241 tatcgcggcg gcgcacgggg agtttgtgct gatgggcgat gcggatgaca catacaattt
     3301 cgcgcagttg gacgaatttg tcgcgaagct ccgcgaaggg tatgacctgg tgatgggcaa
     3361 ccggtttaaa gggaaaattc tgccgggcgc gatgcctccg ctgcaccgtt atctcggaaa
     3421 tccggttctc acggggcttg gccgattgtt ttttaaaagc ccggtcggag attttcattg
     3481 cgggcttcgt gcgtttagaa gggacgcgat cgagcgactc ggcctgcgca cgtttggaat
     3541 ggaatttgcc agcgagatga tcgtaaaagc agcggccttc ggcctgcgcg tcacggaagt
     3601 tccaacgacc ctggctccgg atcgcaggga tcgcccgccg cacctgcgca cttggcgtga
     3661 cggatggcgg cacttgcgct ttctcttgct ttacagcccg cgctggctgt ttttgtatcc
     3721 cggattcgcg ctgtttgcac ttggcattgc ggtgagcgcc tttctgctgc cggggccgct
     3781 gcgaatcggc agcgtcgtgt tcgacgtgca cactctgctc ttcgcggcca tggccatcct
     3841 gattggattt cagtcggtga tattcgcgac gttcaccaag gtttttgcca tctccgaggg
     3901 gttgctgccg gaagatccac gcctcacgcg aatgttccgc tacgttacgc tcgaagttgg
     3961 gctcattctg gggatcctgc tgattcttgc cggggccgga gcatggatcc tcggcttgga
     4021 gtattggcgg attcgcgagt tcggcgcgct cgatccggaa aaaacgctgc gcatcgtgat
     4081 tcccggagtc gtgtgcttca cgctcggttt ccaggttgta ctttccagtt ttttcctcag
     4141 tgttctcggg atgtctcgcc gatgaaagaa acggaagatc tcttcgatca ttacgccgcc
     4201 gcctacgacg aggctttatc gaatgcgatc gctccctccg gcgaaagccg cgaatatttt
     4261 gcggaaggcc gcgttgcatg gctcaagcat tgcctctacg aattaaaatt gccagttcgg
     4321 tccattctgg atttcgggtg cggcgatggc gcaacgactc cgcttctgtt tcaggcactg
     4381 catgcacagt ccgccgatgg cgtggatgtg tccgcgaaat ctctggagat cgcgcgaaag
     4441 cagcatggaa cggagcaaat tcatttcgag tcgatcggag attttcagcc ctccgggcaa
     4501 atcgatctgg cgtattgcaa cggcgtgttc catcacattt taccggaaca gcgcgcgcag
     4561 gcattgtcga tggtccatgg ggcattgcga ccaggcgggc tttttgcctt ctgggaaaat
     4621 aatccgtgga gcctcgcgac gcgctacgtg atgtcacgct gtgctttcga tcgcgatgcc
     4681 atcacgttga cacctcctga ggcacgaaag ctgttgcgtc agggcggctt tgaaattttg
     4741 cggaccgatt tccgatttat ttttccccgg tcgcttcgcg cgctgcgaaa aatggaagat
     4801 tggatctgcc gggcgccgct cggcacgcaa tatcaaatcc tctgccgcaa atccagttga
     4861 gcggacggaa tgagcgacag aatttttatt ggccgccatt ttctgaaatg attgtttgat
     4921 acaaggcttc catttgcgcg aggggttcgg cccaggagag cgaatgcttc acgtcctcgc
     4981 agcctctccg caattgcagc gccaacgcgc ggtcttcgag gattctcgcg agtcctgcct
     5041 caagcgcgtc acactcatgc ggaacaacga gccccgcacc ctgctcgacc atcggcgcga
     5101 ttccgcagcg atccgttact aacactggag ttccgcaggc aattgcttca gccgccgtgt
     5161 tcccaaaatt ttcgttttgc gacggcagga cgaacacgtc cgaatcccgg tacgccgccc
     5221 atttatcatc cccataaagc ggcccggtga ataaaacttg tccgttgagt ttcatcttta
     5281 cggcgagcga ttccagatgc tcgcgaaatc catcgccttc atcgggacca gccatgacca
     5341 gcaccgaacc gcgcgcacca ttgccacgct ggatccagcg agaaaaaact tccagcatca
     5401 ggtccggact tttcttggat accaggcgcc caagaaaaag aatgagtttc gcgcctcttg
     5461 caatattcca tcggtcgcgg aaagaaccag ccgccggaaa atctgttggg acatcgattc
     5521 cgttgcggcg cactacgatt tttgaatcgg gaatcccgcc attcagaaat tcctgccgtt
     5581 cctgttccgc ggttgcaatc acgcggctcg catcacgaat caacctttcc ccaagcatct
     5641 tgtggtagag cctcttcatg cgaatattgc gcacgatcgg caaaaacatg cccatgggtt
     5701 cgacaatgta agggcgcccg attcggcggc attcgctggc cacttgcggc ccgagaaaat
     5761 cgtagagacc atagatatgt gcgagattaa agccgctgag tcgctcgcgg caaaatgcgc
     5821 atacgccggg attccaggtc agcgcacgat atcgcgcgcg cgttcgcaga taaatcgctc
     5881 ccactccatt ttcgttggct tcaaatcccc agcgcgtggg aatcgcgcca atctcgttgt
     5941 gcttttgctg gtccaatccg agatcgacgc ttagaacagt tacgttattt ccggacgacg
     6001 ccaaaccctt ggccaatgca cgcactttaa ccgtaggacc gcccatagca agaaagggat
     6061 aataagattg agtgacctgc aaaaggcgca taagggattg ttttgatgag ctttccgctc
     6121 acatacgaac agccgcacgt gctgaagctc gcggcgggcc cgcttagaca gctgtggaaa
     6181 gtcttatgac ttctccgccg gaaattatac tccggcgcgc tagcaccagg cggcatggtt
     6241 gtgcgttccg tgctaccggg tgagaacgcg attcaggttc gccgtcagct gtttctcaaa
     6301 ttccaggaac gaaaattttt gcgcgacgcg atctttgccg cgcacgccca tttccgtggc
     6361 tcggtcgaag ttgctaagga gagactcgag cgcttgcgca aggttgtcca catcgccgtg
     6421 ctgcaccaga agacccgtta cgccgtcctc gacaatatcc ggaatcccgc cgtgcgcgcc
     6481 tccaaccacc ggcttgccat gcgccatcgc ttccaggaac acgagcccga aaccttcccc
     6541 cttgctcggc agggcgaaaa tttcgcagtg ggcgtaacat gcgaaaagct cgtctggtgt
     6601 gagaccgacg agaaattgca cgctttttga gactcctaga tcgtgtgcaa gttgctcgag
     6661 acgcgcgcga tcgtcgccgt ctcccactag gaccagcgat gtttctggca tcgcttttaa
     6721 tacgcgcggc aatgccaaaa tcagcgtgtc tgcaccttta tatttttcgg cagaattcca
     6781 tcggcctacc gacaaaatgg ctcgactgtt cggcggaaat ccattgggca cggttggtga
     6841 ggcgggcgat gcaagacgcg actcaaaatc gggatcgagt ccccatgcca gccgtcgcac
     6901 tctggagcac ggaatctgct gaagttcgat tagctgcttg acggtatctc tgctcgggcc
     6961 aaggacaagg tccgcgcttc gcagcgcacg gccgcgagtc catccgaggg gctgccaaac
     7021 ttcaacgccg tgcgtaaac
//
LOCUS       DASZMB010000034         5469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_582271, whole genome shotgun sequence.
ACCESSION   DASZMB010000034 DASZMB010000000
VERSION     DASZMB010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582271"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="VG272_01410"
     CDS             complement(<1..1187)
                     /locus_tag="VG272_01410"
                     /inference="COORDINATES: protein motif:HMM:NF004992.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HWF12366.1"
                     /translation="MNSTTLTSSTKRFDAIIIGTGQAGPPLAARFAAEGKTVAIIERH
                     KFGGTCVNTGCIPTKTLVASAYAIHVARRGAEYGFSTGDMHVDINRIKARKDAASARS
                     TKGVEESLRGTKNCTVIQGHARFQSANAVIVNDEILEADKIFINVGGRASVPEMPGIH
                     DVPFLTNSSMMDIDFLPEHFIVIGGSYVGLEFAQIYRRFGCEVTIVEMGPRLIGREDE
                     DVSQAVREILEAEGIHIRLNAKCISLAKRAGGVALGVTCEEGTLDVLGTHVLLAVGRT
                     PNTNDLDLARAGVATDHRGYIVVDDQLQTNVPGIWALGDCNGRGAFTHTAYNDFEIVA
                     DNLFNADHRRVSDRIQAYALYIDPPLGRCGITDAELRKSGRRALAVKYPMSRVSRAYE
                     KSET"
     gene            1731..2954
                     /locus_tag="VG272_01415"
     CDS             1731..2954
                     /locus_tag="VG272_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWF12367.1"
                     /translation="METLTEWLRADRETRKRGVEACLRRIEELDSTIHAWVQVRPEEP
                     TGDGALSGIPFGAKDIVETKGLATEYGSPIYKGRVGTMDAAIIRELRERGAVLLGKTV
                     TTAFAYRTPGPTKNPRNLAHTPGGSSSGSAAAIAAGMVPFTIGEQTRGSIVRPASYCG
                     VTGFKPTFNLLPTEGMLPVSKSLDTLGFFTHTPADMLALWKALDHPVGREETFPFGAP
                     EPMPVCEPEMARAFVQTLKILRQAGIKIVPVDISAMLKKLDEANDVEMFYEGARYHEP
                     RLKEFGDRLDEPLANLVRDGLKIPAQRYNDAKQYIAESKIRFAEIFKSTPVILTPAAT
                     APAPLGLSNTGDPRMNAPWTALGTPAISVPMRVGKALPLGLQLTADLGQDARVLQAAR
                     LLGEHLDAGPEISAA"
     gene            complement(3051..4268)
                     /locus_tag="VG272_01420"
     CDS             complement(3051..4268)
                     /locus_tag="VG272_01420"
                     /inference="COORDINATES: protein motif:HMM:NF023352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3443 family protein"
                     /protein_id="HWF12368.1"
                     /translation="MRATYLSSIYLALGIFSSVAILGCGSSGTTSSPASQVSNQVSNV
                     QPIAVNQGPASNYVDGAFTSVTICVPGSTTSCQTIGGILVDTGSSGLRILSSALTVSL
                     PQQIDAGGHAISECAAFADGVTWGPVQKADVKMAGEVASSLPVQVIGASNFSTIPTAC
                     TAQGAPEDDLGSLGANGLLGIGQAVQDCGLSCSESGFVQNTYFSCPASGCIAITQSLA
                     QQVQNPVASFATDNNGVIVELPTVSGSEATVNGSLIFGIGTQSNNGLGGATVFGTDPF
                     GDFTTTYKTVLYQSFLDSGSNGIYFLNTGVTALPVCTDLTFLYCPTAAQTINVVNTAQ
                     PGTNGATAPASFIAGNGDALVKTSNNAAISDLTGPQPGNFDFGLPFFFGRNVYLGITG
                     KSTPAGTGPFTAY"
     gene            complement(4265..4729)
                     /locus_tag="VG272_01425"
     CDS             complement(4265..4729)
                     /locus_tag="VG272_01425"
                     /inference="COORDINATES: protein motif:HMM:NF022458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2844 domain-containing protein"
                     /protein_id="HWF12369.1"
                     /translation="MKLHLKLAMEMCLVAFTCCLTAHASLGGDMASVHNDQAKLQGTL
                     QTTNKSSYDVHEIQGTSGVVVREYVSRSGAVFGVSWQGRTHPDLRLVLGSSYDQYIQE
                     VQAQRAQRHGHGPLLIQTPGLVVQTGGHMGALTGKAFIPQNLPAGVRPEDIQ"
     gene            5069..5464
                     /locus_tag="VG272_01430"
     CDS             5069..5464
                     /locus_tag="VG272_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12370.1"
                     /translation="MIRYWKYAGIALLALGIIVPAASAHPRVFIGGGFYGPAFYGPGY
                     YSWYGPGYYAAPYAVQASPYLGKVKFDTKMKDANVYVDDAFAGTVKELGTFPLRLGTH
                     NIELRDPSGKILFRDKVNIVAGKTLKLNA"
BASE COUNT         1269 a         1483 c         1539 g         1178 t
ORIGIN      
        1 gtctcacttt tttcgtaggc ccggctcact cgactcatgg gatatttcac ggcgagggcg
       61 cggcgtccag actttcgtaa ttcagcgtcg gtgatcccgc aacgtcccag cggcggatcg
      121 atatataacg catacgcttg gatccgatca gaaacccggc gatggtcggc attaaaaaga
      181 ttgtccgcaa cgatttcaaa gtcattatag gcagtgtgtg taaatgcgcc gcggccgttg
      241 cagtccccca gcgcccagat gcccggcaca ttcgtttgca gttggtcatc aacaacgatg
      301 tagccgcggt ggtcggtggc aacgccagcc cgcgccaagt ctaaatcatt ggtgttaggg
      361 gtgcgtccca cagctagaag aacgtgcgta cccagcacat ccagggtgcc ttcttcacag
      421 gtgacgccga gggcgacacc accagcgcgt tttgcgagcg agatacattt cgcattcaac
      481 cgaatgtgta tgccctctgc ctccagaatt tcgcgcacag cctgggaaac gtcttcgtcc
      541 tcccgtccaa tcagccgcgg tcccatttcc acgatcgtga cttcgcaccc aaaacgtcga
      601 tagatttggg caaactccaa tccaacataa cttccgccaa tgacaatgaa gtgttcgggc
      661 aggaaatcga tgtccatcat cgatgagttg gtgagaaacg gaacgtcgtg tatcccgggc
      721 atttccggga ccgatgcgcg cccacccacg ttgataaaga ttttgtcggc ttccagaatt
      781 tcatcgttta cgatcacggc gttcgccgac tgaaaccggg cgtgaccctg aatgactgta
      841 caatttttcg ttccccggag cgattcctca actcccttgg tagatcttgc ggaagctgca
      901 tccttgcggg ccttgattcg attgatatcc acatgcatat ccccggtgga aaagccatat
      961 tccgcgcctc gtcgggcaac atggatcgca tacgcgctgg ccacaagcgt ctttgtgggg
     1021 atacatccgg tgttcacaca ggtgccgcca aatttatggc gttcaataat cgcgacagtt
     1081 ttgccttccg ccgcaaaacg cgctgccagt ggcgggcccg cctggcccgt cccgataata
     1141 attgcgtcaa atcgttttgt agaactcgtg agggttgtgc tgttcatcgt tgctccgatc
     1201 gagccggcca atttagaaga gcctgaaagc gacggcaaat ttattggacc agcgttttga
     1261 ctccaccgca acagccggca gggacacagg tgcacgaagt agcccttatg atatgccgga
     1321 aacacaatta aaaaacctcg gcgataccat ggtccaattg atcttcaaac gcacgacgct
     1381 caggcgcgcg tgccatttta ctgacaatag gagcaccgcg tgaatctgcc tgggcgtcaa
     1441 ctgcacgcga gagcggcttg gcttcacggg cggtcgccta cgcaccggaa ccactggaca
     1501 aaccggccga cggcaacgtg ctgacttgtt gttcgcagcc ggttggcgac gtcgtagtcg
     1561 atttgtgagc agaccttgta cgatgtccgg cccgggcgta aaatcggctc catattatga
     1621 agcattcaag gcgaaaattt attaggtctg gcgttctcgt ggccgccgtg gctggcgctt
     1681 cgcgagtttc aattttgcag acacgggcag atgcggccga atcgcctcgg atggaaacgc
     1741 ttacggaatg gttgcgggcg gatcgggaga cgcggaaacg cggcgttgag gcttgcctgc
     1801 gccgaatcga ggaactcgat tcgacaattc atgcgtgggt gcaggtgcga ccggaggaac
     1861 cgaccggcga cggggcgctg tccggaattc cttttggcgc gaaagacatt gtcgaaacga
     1921 aaggtcttgc gacggaatat ggatcgccga tttacaaagg tcgcgtggga acgatggatg
     1981 ccgcgattat tcgcgaactg cgcgaacgcg gcgctgtgct gcttggcaaa accgtaacca
     2041 cggcttttgc ctaccgaact ccgggaccga ctaaaaatcc gcgaaatctg gcgcacacgc
     2101 cgggaggaag ttcgagcggc tcggctgcgg cgatcgcagc gggaatggtt cctttcacga
     2161 tcggcgagca aacaagaggg tcgatcgtgc ggcccgcatc gtactgcggc gtaaccggat
     2221 tcaagccaac gttcaacctg cttcccactg agggaatgct tcctgtatcg aagagtctgg
     2281 acacactggg atttttcacg cacacgccgg cggacatgct cgcgctttgg aaagcactcg
     2341 atcatcccgt gggacgcgaa gaaacctttc cgtttggcgc gcctgaaccc atgccggttt
     2401 gcgaaccgga gatggcccgt gcttttgtgc aaacgctgaa aatattgcgc caggcgggga
     2461 taaaaatcgt gcccgtggat atttccgcga tgctaaaaaa attggacgaa gcgaacgacg
     2521 tggaaatgtt ttacgaaggc gctcgatacc atgagccacg attaaaagaa ttcggcgacc
     2581 ggctcgatga gccgctcgcg aatttagtgc gcgatggatt gaagattccc gctcagcgct
     2641 acaacgacgc aaaacaatat atcgcggaga gcaaaatccg ctttgcggaa attttcaaat
     2701 ccacgccggt gatcttgacg cctgcggcca ccgcacccgc gccgctcggc ttgtccaaca
     2761 ccggagatcc gcggatgaat gcgccgtgga cggctctcgg tactcccgcg atttccgtac
     2821 caatgcgagt gggcaaagcg cttcctctcg ggctgcaact tacggcggat ctaggccagg
     2881 acgcacgtgt tttgcaagcg gcacgtttac tgggggaaca cctggacgcc ggaccggaaa
     2941 tttcagcggc ttgagtagca caggcttcag cctgtggaat ttagagcatg caagcgcgaa
     3001 acaccgcagg ctgaagcctg tgctactcaa gctcacgaca cacacaaaaa tcaataggcg
     3061 gtaaacggac cggtgcctgc cggagtgctc ttcccggtga ttcccagata cacattgcgg
     3121 ccgaagaaaa agggcaagcc gaaatcaaaa tttccgggct gcggtccagt caagtcgctt
     3181 atggcggcat tattggaagt tttaacgaga gcgtcgccgt ttcccgctat gaagctcgcg
     3241 ggtgcggtag cgccgttcgt cccgggctgc gccgtgttca ccacattgat cgtttgcgcg
     3301 gctgtgggac aatacaagaa cgtgagatcg gtgcaaaccg gaagggccgt tacgcccgta
     3361 ttcaagaaat aaatcccatt ggagccgctg tccagaaaac tttgatacag cacggttttg
     3421 taagtggtag tgaagtcgcc aaaaggatcg gtgccgaaaa ccgtggcgcc tcccagcccg
     3481 ttattagatt gcgtgccgat gccaaatatc agcgagccat tcacggtagc ttcggagccg
     3541 gagaccgtgg gtaattcgac gatcactccg ttgttgtcgg tcgcgaagga cgccaccgga
     3601 ttttggacct gttgagcaag gctttgcgtg atagcgatgc agccggacgc tgggcaggag
     3661 aaataggtat tctgaacgaa accggactcg gagcaactca atccgcaatc ctgaaccgcc
     3721 tgaccgattc caagcaaacc attggcgcct aagctcccta agtcatcttc gggagcgcct
     3781 tgcgccgtac aggcggtagg aatcgtagaa aaattagacg caccaatcac ttgaactgga
     3841 agcgagcttg ccacttcacc ggccattttt acgtccgctt tttgcacagg tccccatgtc
     3901 acgccgtcgg caaaggcggc gcactcggag atggcatggc caccggcgtc gatctgttgg
     3961 ggcagcgaga cggtaagcgc ggaagaaaga attcgcaatc ctgacgagcc tgtatctacg
     4021 aggatgccgc caattgtttg gcaactggtg gtgctgccag gcacgcagat ggtcacgctg
     4081 gtaaatgctc cgtcaacgta attcgatgcg gggccctggt ttaccgcgat cggctgcacg
     4141 ttggaaacct gattggaaac ctgcgacgcg gggctggaag tggtgccgga agatccgcaa
     4201 ccgaggatcg cgacgctgga aaaaattccc aatgctaaat aaatgcttga gaggtaagtg
     4261 gccctcattg aatatcctcg ggccgtactc ccgcaggtag attttgtgga atgaacgcct
     4321 tgccggtgag cgctcccata tgcccgcccg tttgcaccac gaggcccggt gtctgaatca
     4381 gcaacggacc gtgcccgtgg cgctgcgcgc gctgcgcttg gacctcttga atatattggt
     4441 cgtaagaaga acccaaaacc aggcggagat cgggatgagt gcgtccctgc caggatacgc
     4501 cgaataccgc tcccgagcgg gaaacatatt ccctgactac tacgccgctc gtgccctgaa
     4561 tttcgtggac gtcatagctg gacttattcg tcgtttgcaa agtaccctgt aatttcgcct
     4621 ggtcgttatg gacggacgcc atatccccac ccagcgacgc atgcgccgta aggcaacatg
     4681 taaatgcgac gagacacatc tccatcgcta atttgaggtg taatttcata aggatcaatc
     4741 ctcgcccggg ctgcggcttg taccgccact cagaattccc agcttccgat aagatgcaaa
     4801 ttcgcgccta aatgctggct ggagtcaagg gattggtgcg agcgtgagga atgaggaccg
     4861 gacaggttga aaagcaccaa cattgcgcga aattcaccac gttataattg agcgaatcgc
     4921 ttaagtacaa gtgaaccagg cccccaacca gttgattttt ccctacttat caaatattac
     4981 ccttccttcg tgattcctgg aatctctcgt gcactaggtt ctatcgaatg tgtggaaagc
     5041 gacaatcgga ctcagaagga gaatttcaat gattcgatat tggaaatatg cagggatcgc
     5101 gctcctcgcg ctgggcatca tcgttccagc ggcttccgct catccgcgtg tgtttatagg
     5161 cgggggattt tatggcccgg cgttttacgg cccgggatat tacagctggt acggacccgg
     5221 gtactacgca gcgccttatg ccgttcaggc cagcccgtac ttggggaagg ttaaatttga
     5281 taccaaaatg aaagatgcga acgtctacgt cgatgacgca tttgcgggca ctgtgaagga
     5341 actgggaacg tttccgttgc ggttaggcac gcataacatc gaacttcgcg atccgtctgg
     5401 gaagattcta tttcgggaca aggtgaacat cgtggcaggt aaaacactta agctgaacgc
     5461 ataagtaag
//
LOCUS       DASZMB010000035        47748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_848829, whole genome shotgun sequence.
ACCESSION   DASZMB010000035 DASZMB010000000
VERSION     DASZMB010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 47748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47748
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848829"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VG272_01435"
     CDS             complement(<1..845)
                     /locus_tag="VG272_01435"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HWF12371.1"
                     /translation="MLLISNGRVLDPASGTDAPRDILLDGDRIVAVAPHGQLASRTQG
                     AEIFEAKNLIVAPGFIDLHCHLREPGGESSETIETGTKSAARGGFTVVCPMPNTRPVN
                     DNASLTRSIMERAAAVSPVRVWPIGAASVGSKGEALAEIAAMKEAGIVAVSDDGRPIA
                     TAKLLRQVMEYCRALNLPVIDHCEDSSLFAGAVMREGKQSVKLGLRGMPAATEALAVA
                     RDVQMGELTSARLHIAHLSARSSLELVRSAKAKGLHVTCEVTPHHFTLTDEDVQYDTR
                     YKMNPP"
     gene            complement(832..1671)
                     /locus_tag="VG272_01440"
     CDS             complement(832..1671)
                     /locus_tag="VG272_01440"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HWF12372.1"
                     /translation="MRGKTVALAFFEPSTRTRISFSTAASRLGADQMNLQAESSSLKK
                     GESLLDTVRTLDAMKPDCIVMRHSSSGAPDFVARNLDLPVINAGDGTHEHPTQALLDA
                     LTIRDKRGTLKGLNVTILGDILHSRVARSNIHLLSKFGCRITLCGPAMWVPREMECLA
                     PGAVVRRATRIEEALEGADVVITLRVQQERMHEPSLVADEYVLQYQLTPKRLQLAKPD
                     ALVLHPGPMIRGLEIDPAVADGPQSCVLEQVTNGLAVRMAILFLMIGADTFSERDSSH
                     AAD"
     gene            complement(1845..2393)
                     /gene="pyrR"
                     /locus_tag="VG272_01445"
     CDS             complement(1845..2393)
                     /gene="pyrR"
                     /locus_tag="VG272_01445"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HWF12373.1"
                     /translation="MRIVEKNQLMTATEIDRTIQRLAAEIVEKSGGTSNLALIGIRRR
                     GVPLSQRIAHSMRGIDGVGVPVGTLDITLYRDDLSKIAAQPVLQSSDIPFAVDDKDLV
                     VVDDVLYTGRTIRAAMNGLFDLGRPKRVRLCVLIDRGHRELPIEATFVGRNVQTSDSE
                     IVEVRLNEIDQDERVMLVERVD"
     gene            2504..3466
                     /locus_tag="VG272_01450"
     CDS             2504..3466
                     /locus_tag="VG272_01450"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HWF12374.1"
                     /translation="MLEFAAVTAPNSPNTVELSANVGALRLSNPIIAASGTFGYGIEF
                     AHLVDLNRLGGIVVKGLSLEPMAGAPAPRLCETASGMMNAIGLQNIGVRAFVKEKLPE
                     LRRFQTAVIANVFGCTVEDYVSVIRVLEEADGLAAYELNISCPNTAHGGLQFGSDPQM
                     VLEVVSAARQAAHRPLWVKLSPNVTDISVIAKAAEGAGADALTVANTYQAMSIDVQTR
                     KSKLGRITGGLSGPAIKPITLRLVYEAKRSVKIPVVGLGGIENANDVLEYMIAGASAV
                     EIGTASFSNPAACLNILDQLRKTCINYKINDINGLIGSFQDDSC"
     gene            3541..3948
                     /locus_tag="VG272_01455"
     CDS             3541..3948
                     /locus_tag="VG272_01455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HWF12375.1"
                     /translation="MIWAALRWLPLMFLITQGLEAPLVAHVSQPLKPIRVWTAKTSWY
                     GGKFQGRRTASGEKYDMYAATGASPNLPFGSLVRVSNPKTHMAQLIRINDRGPFIDDR
                     DLDVSYVVACRLGILDSGNTPLRVELLEVPKRR"
     gene            3969..4700
                     /gene="pyrF"
                     /locus_tag="VG272_01460"
     CDS             3969..4700
                     /gene="pyrF"
                     /locus_tag="VG272_01460"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HWF12376.1"
                     /translation="MILALDYSSLDPALEIAQILSSVVGIFKINIHLFTTEGPIAVQK
                     LNQLGPGIFLDLKFHDIPNTVKGAVTSALALPGVQLLDVHALGGFEMMRAAAKVRDEA
                     KVSKSATPKLLAITILTSMDNAALRGVGISSPASRRVVQLARLAKKAGMDGVVASPQE
                     VRAIRKACGKDFLIVVPGIRPVVGGGPTSKSRHKTDDQARVATPAEAIRAGADYLVIG
                     RPITGAPDPEAAAQAILEEIGSALT"
     gene            4870..5088
                     /gene="tatA"
                     /locus_tag="VG272_01465"
     CDS             4870..5088
                     /gene="tatA"
                     /locus_tag="VG272_01465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HWF12377.1"
                     /translation="MGEFSPFHWLIVLAIVVLLFGGKKIPEVMKGLGEGIRSFKEGMH
                     GAGQGPVQTTQVSPPPAPTVTTVTEEKK"
     gene            complement(5161..5751)
                     /locus_tag="VG272_01470"
     CDS             complement(5161..5751)
                     /locus_tag="VG272_01470"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HWF12378.1"
                     /translation="MKIILASSSPRRAEILRDAGIPFKVCAAEINETPLPHESAHAMV
                     ARLAEEKARAAASRVSNGEGECIVIGADTTVELDGEIIGKPDDSVHAREILGRLSGRT
                     HRVLTGIFLLRLPDSATRAAVEISTVTFSPLSENEIVVYASSKEPFGKAGGYAIQGLA
                     GKFIPRIEGCYFNVVGLPLARLYAQLQELGWQNEKQ"
     gene            complement(5870..7507)
                     /locus_tag="VG272_01475"
     CDS             complement(5870..7507)
                     /locus_tag="VG272_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HWF12379.1"
                     /translation="MNPTSNETAKKKARTSERKEQFTTLGGIPLKTVYTEDDLPPGSP
                     EDLIGVPGEFPYTRGIYPTMYRGRLWTMRQYAGFGTAAQSNERFRYLLAKGQTGLSVA
                     FDLPTQIGLDSDHARAAGEVGKVGVAIDSLEDMETLFNNIPLDRVSTSMTINSTAAIL
                     LALYAAVAKKQGADLQKLSGTVQNDILKEFIARGTYIYPPRPSMRIVTDLFAWCRTEL
                     PAWNTISISGYHIREAGSTAAQEIAFTIADAIAYVDAAIAVGLAVDEFAPRLSFFFNA
                     HNDLLEEIAKYRAARRLWAHLMRDRFGAKLPASMMLRFHAQTAGSSLTAQQPENNIVR
                     VAIQALAAVLGGCQSLHTNAMDEALALPTEDAALLALRTQQIIAHETGVANAADPVGG
                     SYAIEKLTDEIEARAKEYITRIDALGGVLRGIETGYVQGEIQKAAYEYQRSIERKEQI
                     VVGVNQFVAQKETPIPTLRVDSDLERAQVERVQALRARRDSSRANAAVTEVERRARSG
                     ENLMPAIAAAVEAYATVGEISEAMRRVFGEYTESVVL"
     gene            complement(7504..7758)
                     /locus_tag="VG272_01480"
     CDS             complement(7504..7758)
                     /locus_tag="VG272_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12380.1"
                     /translation="MATQPMGLFATMRHTFRVLWRAARELFHEVTGAVFFILAFAALQ
                     ACWRAWHNGAGRWLLVTCGAYALVMICFGILSFRDSRRVR"
     gene            7908..8261
                     /locus_tag="VG272_01485"
     CDS             7908..8261
                     /locus_tag="VG272_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12381.1"
                     /translation="MQLDDHLKQLLRELGHAINDTVSESGRISEAISDVRSAGFDIVL
                     KLDATIGLARRDGDDTKITTGDRRFLESLHIQVDQEILNEATEEQESRKMEMTPQDVR
                     FLKSLKITADETQQQ"
     gene            8372..9181
                     /gene="amrB"
                     /locus_tag="VG272_01490"
     CDS             8372..9181
                     /gene="amrB"
                     /locus_tag="VG272_01490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HWF12382.1"
                     /translation="MIRPPAVAGRFYPDDPARLRAEIDSFVFPSIKEKKIPARACIVP
                     HAGYVYSGSVAGEVYGRIDIPKRVILLGPRHYPHGASLAINSGDAWQTPLGMVAVGHL
                     LAEKIVRAFPELREDDVAHRTEHSLEVQLPFLQRLVPFLSFVPIVIGPAQWSTMDRLG
                     RALATVIATESEPILLIASSDMNHYESDAVTRVKDRKAIEQILALDSRKLFDTVREEQ
                     ISMCGYAAAVAVIVAAKELGAIGAELIRYATSGEVNGDMQEVVGYAGIVIR"
     gene            complement(9329..9946)
                     /locus_tag="VG272_01495"
     CDS             complement(9329..9946)
                     /locus_tag="VG272_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12383.1"
                     /translation="MFIPAVHGNPAGTTITYRRVFKGSTPEFIEVKISDQGKCTFDIR
                     QLDEDADPQTFEVGPDVQKKIFDLASDLNHFANQNLDIQKKIANLGQKTFRYENGAEA
                     HETSFNYTMNASATQLMQIFEGLAKQQQDLTLLQRRAQFDRLGVNDALMQFESDMEHR
                     LLPEPERVLPVLDQIAADPHFVEIARTRARALAERIRNSRAPRDH"
     gene            complement(9997..10662)
                     /gene="ruvC"
                     /locus_tag="VG272_01500"
     CDS             complement(9997..10662)
                     /gene="ruvC"
                     /locus_tag="VG272_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HWF12384.1"
                     /translation="MWPDAARRRKLKAVPIAYPTNVEFFRVLGVDPAAAGATGYGVIE
                     SDGRRYRMLRFGALRATRKGPDHFPERLREIHAMLAGLIEEFQPHGVAVEAVFTALNI
                     KTALRLAEVRGVVLLAAAQGGIPVSSYSPREIKASVAGYGNAAKEQMQLMVRALLGMS
                     ETPEPADAADALAVALCHVQAERLRSRLSTGSARTGPEREQPESLGNANSRGAAPRIV
                     SSR"
     gene            complement(10825..11580)
                     /locus_tag="VG272_01505"
     CDS             complement(10825..11580)
                     /locus_tag="VG272_01505"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWF12385.1"
                     /translation="MSGHSKWATIKHKKAATDAKRGRIFTKVIREITIAARVGGGDPD
                     SNPRLRTAILAAKNENMPNENIERALARGTGTLEGEQYEEVTFEGYGPGGVGMIIEVV
                     STNRNRVVSELRHMMNKNGGNMAENGAVGWMFSRKGALVVSKEQADEDKMLSIVLDAG
                     AEDLRDDGEAWEVLTPPERFEAVRDALAKANVKTESAEVGWVPQNYVKLAGTQAAQML
                     RLIEALEEHDDVQKVSANFDIDEKEMQAVMAAE"
     gene            complement(11754..13604)
                     /locus_tag="VG272_01510"
     CDS             complement(11754..13604)
                     /locus_tag="VG272_01510"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWF12386.1"
                     /translation="MRSFHVRSRWILVFLLLLSCGLYIGTAGTPALDDDDVDAAHAMV
                     SQEMLQRHDFVVPYMDGIRYLIRPPMHYWLVAASYKLFGENEFATRLPLALAMIGMVL
                     LTFEFGCRFFGQRAGLYAALAVATSAGMFIFTRVMIPEAIYALAFEGIFYLFLRSWSG
                     CLDSRIGYWGAAALCGVAVMTRALIGLLFPAVAIVTFITITGGWHRWRELRLFSSTAI
                     FLAVAAPWHILAALRTPGFFWAYFINEHINRALGTRLPHDYGAVPLGIWWSSHLIWLF
                     PWSFFFPLALRNAPLLPSSWREANTPKRQARLLLFVWAAAILGFFTLESGSRMEYYSF
                     GAWPAIALLLGYGIAQAEESDQFWLKPIQRVLAGVAVVFAAISGYLIIWTLKIQAATD
                     VSSHLALRSPEKYMTSMAHVLDLTPQSVSDLRIPLLISSLSILIPFVLAWLWRERGIS
                     WYPNFALAAGMVGFFIAANIAYGVLSPTLSSRALALELNKVLQPDDQIALFGDIRVAP
                     GIAFYSHRDVMLYDAAESNLRFGSKYLDAPKRFFDDKDFSRLWVGPSRVFLVVPDEHL
                     EEVRDRLPPNSVWEFADTGGKAVYMNQPIEPGQPAITSSEVKIEVNPILK"
     gene            complement(13811..15655)
                     /locus_tag="VG272_01515"
     CDS             complement(13811..15655)
                     /locus_tag="VG272_01515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HWF12387.1"
                     /translation="MENTVESANDIRWDLSILYTDIADPRLDADLQELKAMAKHFSDS
                     YKGKLAEQLGAAIRDYAEIEMLSGKMNSYLFLRESTDLTNAAIKAKHAAFEREMSAVQ
                     GEHLTFFELELVQLSDETLNKFYASDSLVSKHRPWIEHIRTFKRHFLSEAVESALVKR
                     SPFNSSSWSNFFDEVEADLNFEFQEGTKNLTEMMHLIAESKDSNERAEALQCVNSGMA
                     GPFAKYSAQTLYMVAGLKAVEEKERGYAHPMDGRNKSNRIPDSVVNVLHNAVTNLGGT
                     LTRRYYKLKARHLGLTVMRWSDRNAPMPFADTTRIPFDEAAGIVKKSYESFSPTLTAL
                     VKTFFVERRIDVPVVKEKRSGAFNSSHVLPGGKAVSFTLLNYLGSNRDVMTLAHELGH
                     GVHGILAGEAQGPLMFHAPIAYCETASVFGEMTTFTFLKERLMHSGDKSSLLALIMAK
                     IDSTINTVVRQIGFSNFERRLHGMDETYSTWSEPKRRSVEELDAIWLKTAQELYGKDG
                     DIFTYENSEHLWAYIPHFHSPFYVYGYAFGELLTQSLFAQKEKLGAKFEPLYLDLLRS
                     GSTKDVVELLAPFGLDARNEAFWNDGITVSLGALIQEAEQLSREMGIV"
     gene            15921..16268
                     /locus_tag="VG272_01520"
     CDS             15921..16268
                     /locus_tag="VG272_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12388.1"
                     /translation="MPTVVACSEKGCKGRVPAALEPVHSCVLHFTLKIESECAQMRRE
                     TVYGRTPHARQVEIINFIADNGEMLARASTSGLHLADEMKARILNTFLTLMNLRENLD
                     RAALRQPIGRTSG"
     gene            16512..17003
                     /locus_tag="VG272_01525"
     CDS             16512..17003
                     /locus_tag="VG272_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HWF12389.1"
                     /translation="MKRHSSLHPLSQHHHFALIQALEMRRAAEMPLETRAAHIEKHAA
                     KFVRFWHKTGLVHFREEEEILLPAYSRHTRLDRDADVMRILADHAEIRAAVLDFEQRL
                     AAKIPVEAEQMAHLAKLLHDHVRFEENVLFPRIEKTLGEEGLNKMGRGLTRLHSKNDP
                     CEI"
     gene            complement(17152..18237)
                     /locus_tag="VG272_01530"
     CDS             complement(17152..18237)
                     /locus_tag="VG272_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HWF12390.1"
                     /translation="MLVFTGPGMREALGSMDAKTYDRYSRQILFSEIGEAGQARLLES
                     SAVMVGCGALGTALANLLVRAGVGKLRIVDRDFVEPSNLQRQTLFEESDAQEALPKAV
                     AAERRLRAINSGVVVEGIVADVSSKNAEEFLSSFPLILDGTDNFETRFLLNDVAIHLH
                     VPWIYAAVVASYGVTLTVRPGETACLACALESESANGSQSMGSEDTCDTIGVLGAAAG
                     VIASIEAAEAIKLLTGKPELTGGRLNSFDVWSGKFQSVRVARNTDCRACVRHDFRYLE
                     GETQPHLTMCGRDSVQIHERRRQLDLSALGKRIAPTVAEVRENGFLLRFRVPPYEMTV
                     FADGRAIIKGTQDSSIARSLYARYIGV"
     gene            18435..21449
                     /locus_tag="VG272_01535"
     CDS             18435..21449
                     /locus_tag="VG272_01535"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HWF12391.1"
                     /translation="MPIVHCPFIRFAAWLATLWLLCASPLAAQNNVAGDPPRVVAVRV
                     VTESGAVLEENPPKLTLQPGQPFSTESESVSLRELFRSGKYADLRAELADVPGGVRLD
                     FVVRQNLYINRVQIDGLHEPPGEALALSALRLNVGEPFRISDMKDALDRLRQTLEDDG
                     EYQSKLTYITTPHPETLQMDILVHVMEGPRARVGAITIQNQTEFPEQELRNQLKIGEG
                     SQITVERLNRVADRARKWLTGNNYLGARVTIHRGTYDATTNRVPLNFAMYAGLEVRVA
                     VEGARISSGNLHKLIPIYQEGAVDQDLLQEGRRSLRDWFERAGYFDTQVNYTVKEAPT
                     GKSRNTTHRTASVVTYDVNRGDHHRLVKVEFDGNKYFSSDLLRGRLKIFPAGYASPGH
                     YSTEVLQDDVASIRTLYEANGFEQCQVQSQLVDNYRGHNGDLSVTFEVQEGLQTRVAG
                     LTIEGNAQLSLDELLGVVGSSKGQPYSEFNVSSDRDNILATYYDQGFSEAQFTANVEK
                     IPPAAGPNAGPTVRLTYHITEGRQVLVARVLLSGYEHTRPGVISREVGIKAGEPLSEG
                     AVVETQRKLYNLGIFSRVSIAPQNAEGLDEKKTIVVMVDEAKRYTIAYGLGFEAQRLG
                     SSTNAADQSLNFAPRGTLELTKANLTGRADSLSFKARASTIQGRALLTYIAPNYFASP
                     DFSLQLSTFYETSRDVQTFDSRRAEASAGLAEKLSPSSTILYRYVYRHVVASNLKIAV
                     EEIPLFSQSTEVSEFDVNWLRDRRNSPSDPSRGDFENVDISLAAKAIGSSANFIRVYM
                     QNSTYRPIGRRLVFARSTRFGIQTVYGNSLSTDIPLPERFFAGGGTTIRGFGLNQAGP
                     RDPFTGFPIGGQAELIFNQDLRFPMHLPLIGDRLGGAVFYDAGNVFPSIRRMSFRIAP
                     PAPTFDPANPNVCLTNCTNDMNYFSHTAGFEFRYSTPIGPVALDLGYQLNPAKYLIQS
                     GGTCPPSQAAACLTTTTRLPAFQFFVNLGTTF"
     gene            21449..22108
                     /locus_tag="VG272_01540"
     CDS             21449..22108
                     /locus_tag="VG272_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12392.1"
                     /translation="MRRVMIIFILLFAAPETPRAFAHVPLPQRVVDRIVARIEDDIIL
                     QSQVRELGAFQQLIDGQAESDDKLLQELIEQWVVATEATASHFPQPAEFEVDREMERL
                     VSQFANMEKYQARLRELGISDNQVRQLLIRQIYDERYVDYKFRPSVRVEAPDVEEYYK
                     KDLLPELAKKNQPTPAFATVEEQIRELLIQRGISSMTLKWLDDTKSRLRIEIETPNGN
                     P"
     gene            22131..26102
                     /locus_tag="VG272_01545"
     CDS             22131..26102
                     /locus_tag="VG272_01545"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HWF12393.1"
                     /translation="MSGTKWKFGLKHGLGLFVGLALILVLAIAYVIANGFADQWARRT
                     IVEQLEKATGARVELGNFHITWRKLTARFDGLTMHGREPAEGPPLFHADKLEFDIKID
                     SFWGRKISLANVEMSRFSANIQVDKDGSTNIPGPKVVVSTGAPDLRNLFDLKIAQLHL
                     DEGEIIWNDKRIPLEAKGGQFDFAMNYVTEGGLPVYLGQVSWQQFQVAALHNLPFSTS
                     FTSRFSLRQTSFSVTQLQWKTPGGEIDAQANLPSFAQPAWTFRYRGQARLEDLWTILR
                     QRGAPGGHIDFVGEGNYSADQMNFTGRYTADRVTMKYQWFHPGNISAHGSYRANDKTI
                     DLPDLEALLIGGHVTSHVSVDLAKLEFRAESKVRGLSLRQALATEDNPSLPINPLHWD
                     SSMDVDATTTWIAGFKHVVSKGSSVWTPAVNPAPGTIPTAANFEFNFNMDRNQFELSP
                     GEITTPTSRIQFRGVLSMINTSLDMTVDTDNLTLWDDFINRLRGADSTPEIMKGQFHW
                     EGRLLGPLDGPTFAGHFKGMDASYGPYYWDDLEGELTYSPAELLLERTRARRGQSSAD
                     LELALDLDNWGFTADRHWTFDVNLVRIDTDDLQKMLGTSYDARGVLTGQFHLKGTRAQ
                     PEFNGLFDLSNTKAGSWRFERARGQLSIHAGEIRIANAEVRLPANTPGAAAGIVTGNL
                     DYHTDSGEATFDFTGAAIPLESIGKIQSARLPIAGRLNVQAHGQGLLRAPELHATLRL
                     IDLKLGNDVVGSFDGKADSDGKHLHATIDSAMASGKLSGKLDIGLQGDYPVSAQVIAQ
                     RIDFNPFLVSALHLTRLEGSSLVDGQFDVAGFGGRPETLAVESNLSRLTFDFRSVKLE
                     NVGPIRLTYRRDEVRIEQANLRGTDTDFHLAGSARFTQDQALNLRLDGAVSLQLLSSF
                     YSQLEASGRAQINAAVAGTVATPRFNGKVHLEGASLRYGDFPTGLSNVTGDLNFDVNR
                     MVFDNVVAESGGGHLTIGGTVAYGGGPLNYVLNARSDQIRIRYPVGMSWLVGGTLRLS
                     GNSQAATLSGRISVDRLLMAEGFDIASIMASSKEPASGPSTTSSFLQNLQFDVQAESS
                     PDSRMEWTGARLQTDASLRLRGTWEHPILLGHIHLLSGEMNFRGNQYTISRGDINFAN
                     PFRLDPVMNVEATTTIRQYEVTLDFTGPASRLTLAYRSDPPLPSSDIIELLALGQTGE
                     ESAARTATGQNSSAGASTLLSEAISSQLGGRIQRLFGISHFSVGPSVATLNTQQNSIA
                     SVTIEQQVTRGLVITYITDVTTTQYQVIQIEYTINREFSVVALRDENGTFGLDVVRKT
                     RFK"
     gene            26454..27053
                     /locus_tag="VG272_01550"
     CDS             26454..27053
                     /locus_tag="VG272_01550"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWF12394.1"
                     /translation="MAQVWAKYVKFFRVSIIVAAALVAVAIPAGAQKKDKNKTAPTPD
                     PTEELKTAMRGPDSQFIDQTIGEALGYWQIGDTESLRKYYAEDVVVVSGAWEPPVIGW
                     DNFLKAYQAQRAQINGARMDRINTLIKVNGNSAWATYQFTYAAQMNGVVVQFRGHTTL
                     CLAKQADRWVITLNHSSIVDSTAPQPTTSTGPPQTTGKP"
     gene            complement(27050..27334)
                     /locus_tag="VG272_01555"
     CDS             complement(27050..27334)
                     /locus_tag="VG272_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12395.1"
                     /translation="MELTRTVLLNQLMISWIVITVVLIALVVYRATLSSREDDQIFID
                     AAEQHHYQEQIAIIAQMSRLTKPIIALSVLSGALLLASAGVWIYSGFMSF"
     gene            complement(27452..27742)
                     /locus_tag="VG272_01560"
     CDS             complement(27452..27742)
                     /locus_tag="VG272_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12396.1"
                     /translation="MRVPVEIRGTAADGTAIEESTYTGVVGVLGAMIWTSKALQTGAE
                     VELTNRFSQRTAKFRVAWVKEPQGTELWETGVESLQPLDDFWGVRFPPKADA"
     gene            28025..28111
                     /locus_tag="VG272_01565"
     tRNA            28025..28111
                     /locus_tag="VG272_01565"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28059..28061,aa:Leu,seq:cag)
     gene            28295..28714
                     /locus_tag="VG272_01570"
     CDS             28295..28714
                     /locus_tag="VG272_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12397.1"
                     /translation="MTRDIIFATDESRAESGKATKRIFQIAYTEALRISRSEILRSLG
                     YPVVSVIGNEAAMTLLTTLRRDNLDIAVFIVGHAAPADTRKEMVDWLKANYPTAKVLA
                     LNSPDQQIELADYNVKQNGPESWLPLVTLNISPLQAS"
     gene            complement(29137..29928)
                     /locus_tag="VG272_01575"
     CDS             complement(29137..29928)
                     /locus_tag="VG272_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12398.1"
                     /translation="MEESLRAQIDSNLSHLDHLIRRGNKLRESLSADPTNKSLIVANR
                     AWQQDCGVAMNQLSGGSKAHWLARAFSEAFLLRTDSGEAIDLVPPAVIVERLVHVLDQ
                     AVLSLSRMGSEENSAASASATPQPRRFEFVHNVEIRPVLEQAYADSRDALERREYEEA
                     LTNSCGILEAIVTDALEHNGVDAMACADDLNGKISDWTFHERLIVAERAGLIRGGCAR
                     LPEIARGYRDSMDANGNANLQVTEQDARRSMQVLHVIMRDLDPGR"
     gene            complement(29944..30618)
                     /locus_tag="VG272_01580"
     CDS             complement(29944..30618)
                     /locus_tag="VG272_01580"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HWF12399.1"
                     /translation="MADRIRVLMLDGTFPPGEPLPSERHLAERFGVSRGSIRDALRTL
                     ETIGLVETLHGQGTFPLELSVDRLVAPLASVMSYRADLQDELLDVRRMFEPAVARVAA
                     LRASAEDLAELQKILDAQRLKLKTGQSAISEDTAFHAILARATRNRVVMSIMATLNDL
                     LVESRTSSLLQKGRPARSIDGHEAVVAALRRRDAEGASQAMYNHIDQIADIQLHAKKG
                     SDSPTK"
     gene            30769..31110
                     /locus_tag="VG272_01585"
     CDS             30769..31110
                     /locus_tag="VG272_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12400.1"
                     /translation="MSEKLFEFMTAGSPRAVSGAIEQFAIGLGSLTAVVVPWESDKNS
                     LCMAVASTQGEGWALEHTNIGTLRLVDLGNENTRIEIFSELKDHAEQQKLANLFARFS
                     SQLQSQFQAAQ"
     gene            31107..33323
                     /locus_tag="VG272_01590"
     CDS             31107..33323
                     /locus_tag="VG272_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWF12401.1"
                     /translation="MTELTPSTPATTLTLRVAEARVEDVGRAIARLAPSDLQRIGAQS
                     GDVVKVTGGTSSVARAEISDEKHEGFVQIDGTARSNCGVGLQEQVNVTPVEHSQALAV
                     RLSPMWIGAAPAIIAVERIVEDLTGVPVTQGCIVRVPTFAKAVNFQVVRTIPSGPVII
                     GAKTDIRIVEGEQTAVRAPAVSYEDIGGLEREVARVREMVELPLKHSRIFERLGIFAP
                     KGVLLYGPPGTGKTLLARAVAAESRVHFISLNGPEIMRKFYGESEAKLREVFEEAARR
                     APAILFIDEIDAVAPKRAEVSGEVEKRVVAQLLGLMDGFVSRGQVIVIGATNIPEVLD
                     PALRRPGRFDREIEIGVPNTQARLQILKIHTRAMPLAPDVNLQEIAEHSHGFVGADLE
                     ALCQEVGMIALRGFLATAPLETDGAPPVDLSGLHVTREDFLAGLKEVEPSATREFFIE
                     KSTTTFADLGGNTDLKRLLDSVVEHSHMHNEIYDQVGLAPPRGILFVGPSGTGKTAMA
                     RALSGEKQIPLIAIDGPQLYSKWLGESERALREVFKKARRAAPCILFFDTIDALAPKF
                     GVDHLGSDIYQRILSQLLREIDNLRDVKGVLLVAATNRPERVEPALLRSGRFDYILPF
                     AKPGAADRAEIMRLCCRRVPLAPDTDVDGFASQLEGSTGADIESLCKKATLLAIAKFQ
                     DGAQDAAFTVRRSDFQSVLDSHRGSPAQMKDLAARSGNAEGFTLARIHLDDKEVRS"
     gene            33320..33967
                     /locus_tag="VG272_01595"
     CDS             33320..33967
                     /locus_tag="VG272_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HWF12402.1"
                     /translation="MSYTELTVWTRGIIMDKEGRDIVNSVGASARTEGKFSQSMENYV
                     DNPDRTNAPTRKYCRVSDSVIENALTYENEHPNIVVLVEGTMVKGWDYMRGMPPGGTL
                     IINTHYTPEYMIRFVPGAERLAQLVCVDAAKLADHKWLYYRLGELGLDRLSTEGAAER
                     SKLVAPDIAAPLIAAVVKTTGIVKMETIEPMIANKAAFHAALKELHIMKLNPVTA"
     gene            34044..35876
                     /locus_tag="VG272_01600"
     CDS             34044..35876
                     /locus_tag="VG272_01600"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWF12403.1"
                     /translation="MSTKGTHIPDWLNVPFAGITPAPTQEKGQDLFPTGNWRAIRPVY
                     RDKLPPCNNACGTNEKIQGYLDLVKRGKYLDAYALIKEDMPFPSVTGRVCYHPCEQAC
                     NRGQYDEAISIRAVERFLGDLGQALAKDVVKAGPSNGKKVAVIGSGPAGHSAAYQLAR
                     MGYKVTILERSPKAGGLNRGGIPDWVLPQAVLDREIQRLVELGIEIKTNVEVGKDITL
                     DDLKKNYDATVFAIGLTESNSARAEGENKAGVIYGLPFLRDIGMGTSKVKLGPRVAVI
                     GGGNTAIDCAREALRQGAIEVSMITVEGIKDEMPASPEDLHDMLEEGVELMHGLAMTS
                     VLGDGKVEALQLHPARFTGKINASPIAIDREATAKKFAVDNVIIAVGQRAKLDWMPAE
                     YKTERGTIKIDEFGRIANTNFFAAGDVVQLGSGQPLMVVNAVGDGKRVAFNLDKVLRN
                     APLEPRTVPIDVIVELARMNMTYFPHFPRVQQAMLAPAARKSTQQEVIQAYSEEEATA
                     EAARCFSCGTCNACDNCYLVCPEPCIVRSVRSNGLYKILVDYCKGCRVCIEECPTGCL
                     EGVPELDFDTGVVRMDTAFAISQGLHGRQAEELAHLSDRPAKNV"
     gene            36026..37234
                     /locus_tag="VG272_01605"
     CDS             36026..37234
                     /locus_tag="VG272_01605"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12404.1"
                     /translation="MATLVAGKKKTVRINGCVASAQAAKYADVDVITAYPIRPYTATM
                     MALAQMVANGELDAEYIVADSEHSQLSIAHGASSSGARVFTGSSGVGVQYGYELYSPI
                     SGSRMPVQMMIADRTLDPPGDFGSEHTDALSTRDMGWLMGWSCDAQEAFDNHLIGYRI
                     GEDPRVLLPQMVCQDGFFVSHIVCDVDLPDQGQVDEFLPPYKHPYPLDPRHPVSHGPQ
                     IMPEQGPPLQLERARAMEGSMAVIEQAHEEFAKIFGRKYDPWIEEYMTEDADVVFFLQ
                     GAHAVTARYAIKHMRDRGAKVGLVRLRTIRPYPTEQVKESLSKFKVVGVIETNMGLGS
                     VSSGGSLYAEAAAALYESQKRPLIVSFMAGMGGEAIVLKEFYWMTQKMLEAQKRGSIE
                     KRTHWVGFEE"
     gene            37338..38402
                     /locus_tag="VG272_01610"
     CDS             37338..38402
                     /locus_tag="VG272_01610"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWF12405.1"
                     /translation="MSTIEIERQILPMFGSPNQGARFYNPELPSSEPFVPGHRTCAGC
                     GPSIQYRMTSKASGDNTILVGPTGCMYVANSSYLCTPYNVPWAHTQIGSSGSFTAGVS
                     AAYEAMIRKGKYKGKFPNVICEAGDGSSSDIGIGSISGALYRNHDCLIICYDNEQYAN
                     TGIQASSRTPYGGMTTFSPPGPKVPEAKTLFPKDLARMMAAGHPHCYVATASVGYPID
                     LMNKVRKGLNFKGAAFLMVYTPCQKGFVYETPRSIDLGRLVVECGLYPIWEWDNDKRA
                     YDYSFKPQSMRPVSEYLKLQGRFGHLHAEHIATLQKFANQQWQMMGVQLPEALIKATD
                     VKNLSNMGSAEAAASLAETV"
     gene            38399..38770
                     /locus_tag="VG272_01615"
     CDS             38399..38770
                     /locus_tag="VG272_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12406.1"
                     /translation="MSTNPSSNAPAAQQEAYIVSEGKKRGSTREVRDEAAFDRYYTVV
                     KVHAGDANLTDNWHTNEVLSHAKNNFEGYTFVMRTLEAWGAMIQNDHRAYYTAGGRVW
                     VLDLEPATGKKPRLPGNYGTK"
     gene            38778..39164
                     /locus_tag="VG272_01620"
     CDS             38778..39164
                     /locus_tag="VG272_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF12407.1"
                     /translation="MPRILLIDDTPEIAQLLSFSLRDHGFEVFASGFTDSINELVAEQ
                     KADALVLDCSVYDMSETLFDTVRFDATNAQLPVVIISDTPEEADKSLRSREATNVMLI
                     PKPFTGSHVARALTELLKSREARGQG"
     gene            39167..39490
                     /locus_tag="VG272_01625"
     CDS             39167..39490
                     /locus_tag="VG272_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12408.1"
                     /translation="MASWNMDNWRYVERQSVTDPKGTQWSVALMDILGQEGDPEMPNK
                     LQELQYSSGRFFTLVYSASGSVVWERGYGTLPEATKAFERLIVDVMDGVVDPTQPTFR
                     EDLED"
     gene            complement(39548..41146)
                     /locus_tag="VG272_01630"
     CDS             complement(39548..41146)
                     /locus_tag="VG272_01630"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWF12409.1"
                     /translation="MRKKIDFRIPGKFFSNRSARRFGKQWPFVAVALLFATPAFAQLG
                     PPSGSSQGTQAVQLPLSGRTAQSGGTVKTSEAPAPSTTATVNTLSPSVNVQGNYAGST
                     PGTAKMPFNGKLGFKDAIQRGLSFNLGESGATQTMRQAEGQRKVALSSLLPNINGTVT
                     ENVQTTDLRAEGFRFNFPGFAIPAVIGPFNYMDLRAHLSQNVFDLAAINNFRAANDVA
                     KANRYSAQDARELIVLAVGGAYLQTIAAKARMDSEQSQLDSANAVFDQSKQQLGQGLI
                     AKVDADKNEVQALTHQQRLLSLKNDLAKQKINLARMIGLPPNDQYELTDDVPFAPTEP
                     LTMEAALAQAFQQRADLKAADAQVHAAERSHAAARAERYPSLGVTGDYGGIGVNPSQL
                     QTTYTATASLKIPIWQGGRTEGDIQQADAALAQRRAEYEDLKGQIESDVRSAYLDLEA
                     AASQVEVSQRNVQVTKEALDLTRQKVEAGVIDNVQFVQAQEQVTIAEFDYINGVFAYN
                     IAKLNLARAVGRAVDGLPEMFKAQ"
     gene            complement(41285..42451)
                     /locus_tag="VG272_01635"
     CDS             complement(41285..42451)
                     /locus_tag="VG272_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF12410.1"
                     /translation="MNKIRAFLGYRMRALIRKEFRQILRDKRLAISLILPPTLQLLIF
                     GFALNATVSNLRMGVVDDSKTPESRELVATMTESKSFKGAGTYFDINELSKKIANGNL
                     DAGLVIPYDYARDLQRGQPATVQILLNAMNSNTATIGQGYAQGVIASYNQTLAGEGIH
                     TKFQQIAAPDVSHLGMVLLHPAYLYNPGLEPTWFIVTGILGVLLILNGSLISAAAIVK
                     EREAGTLEQLLMSPASTTQIIVAKLFPLFVLLCMMGVLAVALMRIVFGIPFRGNVLLV
                     IAAAALCLLCGISIGTFIATFTKSARQAQLTLFFVNPPLTSLSGAFTPVEAMPKWLQP
                     LAQFNPIQHFGMISRGAMLKGSGMDTLWPNFLALLAFTIALVALSIWRFRKQLS"
     gene            complement(42455..43561)
                     /locus_tag="VG272_01640"
     CDS             complement(42455..43561)
                     /locus_tag="VG272_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF12411.1"
                     /translation="MRRIIAQARKELIQLLRDRLAVALALFLPVVLLLLQTTAISLTV
                     KDLPIIVQDLDQSQASQKLVDTFRQSLSFHVTSWPADREPEEAFASNSARGALIIPEE
                     YGREIARGENATVQILVDASDENTAQIISGDAAQIVRAYNAENAGARQVQPVNAAIRL
                     WYNPSRSSQKFYGPGIYVLAMSLFPPLLATLAMSKEGEQKTILQVYVSNISAHEFLLG
                     KIAAFMLVATAEAIVLMMLLFMGFGLRFAGDPTPFLVSTILYMFVVTTFGNMIGAAIP
                     SQVAAMQAVALTGFLLVYMLSGLLFPVENIPAGIRWISNIVWGRYYIEIVRDSLLAGG
                     GWPSVWSKVLAIAAIGAIFYTIAWRNLRHMQLKD"
     gene            complement(43573..45555)
                     /locus_tag="VG272_01645"
     CDS             complement(43573..45555)
                     /locus_tag="VG272_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWF12412.1"
                     /translation="MNTAASDRENPPAPSARASDVAIDIRNLKKNYGTLEAVRGISFD
                     VKRGEIFGLIGADGAGKTTTFQILAGVMEASGGSAEIFGKPAREARSRTGYLTQTFSL
                     YPDLTVLENIRYIGDLRHVPPEEIGERGGRYLKMFDMDRFSGRLAAQLSGGMKQKLAL
                     VCALVSEPDVLLLDEPTTGVDPVSRREFWDTLAHLAGQGLTVLVATPYLDEAERCNRI
                     ALIHLGEIQQVGTPDDFRESLHAKRIELRSPDLRKALQAMGKVSGREGAIFDVQRFGD
                     RLDLLSHSPDAARQKIEETMRREGIAIDSVRMDEPTLENTFVATLRSLGGEMHDTPFP
                     ARHDHKAKRGQVAIGAEHLTKQFGSFTAVHDVSLQVRYGEIYGLLGANGAGKTTTIKM
                     LCGLLEASTGNMQLAGERGAFRSTDIRQQIGYMSQRFSLYDDLSIGENLDFFGGVYGV
                     PSEELTEKERWVLDFSGLQGKEQLITGSLPGGWKQRVAFGSAIMHEPGIVFLDEPTSG
                     VDPLARRAFWMMINHLADAGTAVLVTTHYLEEAEQCNRLGFMVAGELLVEGSPSEIKA
                     QQKGHLLEFRVDQPQRAADLLKTETQHWRVALFGNRLHIITDNDVETGIREYTQRLSA
                     GGIRVIDAREGRFSLEDVFISVVEKAREHGKIGSEE"
     gene            complement(45552..46853)
                     /locus_tag="VG272_01650"
     CDS             complement(45552..46853)
                     /locus_tag="VG272_01650"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HWF12413.1"
                     /translation="MDETKNNTNTMTQAPPSTEQRTPPPPHQASAPPEVNKEPRSRVS
                     TILIILGVIVAAVLIWELFFATPNLPASIVALSGRIEGDDSAVAPKTNGKILEITVRE
                     GDTVTAGQVIARLDDAQVRAKEDQAKAALLDSQAKLQSARDQIAILQEQLTENQLQTG
                     QSTMDAQGRVRQAQADLTAAEADLVQQQAALRLAEFNRDAYARLSKTGAASQLQGLQA
                     EVQADQQAAVVASTQRKVEGARGALTTAQANLDTPGIRRAQVSGTEAQIAQQQSTIAS
                     AKASTAQAQATLAQAQADRADLTVVAPFSGTVLTRAAEPGEVVQAGTAIVTLLDLSKV
                     YLRGFVPEGNIGKIKVGQPAHVFLDSNPNQSLDGYVLRIDPQATFTPENTYFRDDRVK
                     QVVGVKVQLTQGIGFAKPGMPADGEILTNGNAWPPHKRAAQ"
     gene            complement(46846..47502)
                     /locus_tag="VG272_01655"
     CDS             complement(46846..47502)
                     /locus_tag="VG272_01655"
                     /inference="COORDINATES: protein motif:HMM:NF020771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HWF12414.1"
                     /translation="MVRKRSMHKAPELTRDKLIDAAGHVFAERGYRATTIREICRRAG
                     ANVAAVNYTFGDKMGLYTEVLRHSVRAAKTAAMNAALDTALSPEDTIRGVIRARLMSM
                     CQEERPDWHIRLVMHECSQPTGAMQRVVDEGMRPIYDRMKKAVGQVIGLPPDHETTRL
                     SVNSIVGQILFYTFSRPVLAHLQPELKLTPNQLDRIADHIADFSLAYLKKVGHGKRHG
                     "
BASE COUNT        10768 a        13256 c        12999 g        10725 t
ORIGIN      
        1 ggcggattca tcttgtagcg cgtgtcatac tgcacgtctt catcggtcag cgtgaagtga
       61 tgtggcgtca cttcgcaggt gacgtgcagt cctttcgctt ttgccgagcg caccagttcc
      121 agagaagagc gtgccgaaag atgcgcgata tgcagacgcg cactggtcag ctcgcccatt
      181 tgcacgtcgc gagcaactgc gagcgcttcc gtggcagcgg gcattccacg cagccctagc
      241 ttcacggatt gctttccttc gcgcatcacg gcgcccgcaa acagggaact gtcttcgcag
      301 tgatcgatca ccggcaaatt caaagcgcgg caatactcca tcacctgtcg caaaagtttc
      361 gccgtggcaa ttggacgccc atcgtccgaa acagctacga tgccggcttc cttcatcgcc
      421 gcgatctccg caagcgcttc acccttgcta cctaccgacg ccgcgccaat gggccacacg
      481 cgcacgggcg aaaccgcggc ggctcgttcc atgatcgagc gcgtcagcga tgcgttgtca
      541 ttcacaggcc gcgtattcgg catcgggcag acgactgtga agccgccacg cgctgcggac
      601 tttgtgccgg tctcaatcgt ttctgacgat tcgccgcccg gttcgcgcag atggcagtgc
      661 agatcgatga aaccaggcgc gacaatcaaa ttttttgctt cgaaaatttc cgcgccctgc
      721 gttcgcgaag caagttgtcc gtgcggcgcc acggccacga tgcgatctcc atcgagcaga
      781 atgtcgcgcg gcgcatccgt cccgcttgcg ggatcgagaa cgcgcccatt gctaatcagc
      841 agcatgagaa gaatcccttt ccgaaaatgt atccgcgccg atcatcagga acagaatggc
      901 catacgcacg gcgaggccgt tggtcacttg ttcgagcacg caggattgcg gcccgtcggc
      961 aaccgccgga tcgatttcca atccgcgaat catcggcccc ggatgaagta caagcgcatc
     1021 gggcttggcg agttgcaggc gtttcggcgt gagctggtac tgcaggacgt attcgtcggc
     1081 gaccagcgag ggctcgtgca tgcgctcttg ctgcacgcgc aaagtgataa caacgtccgc
     1141 gccttccagc gcttcctcaa tacgcgtggc gcggcggacc accgcgcccg gcgccaggca
     1201 ttccatttcg cgcggcaccc acatggcagg gccgcagagc gtgatgcggc agccaaattt
     1261 tgaaagcaaa tgaatattcg atcgcgccac gcggctgtgc aggatatcgc cgagaatcgt
     1321 gacattcagt cccttcaacg tcccgcgctt gtcgcgaatc gtcagcgcat cgagcaatgc
     1381 ctgcgtggga tgctcatgcg ttccatctcc cgcgttaatc actggtagat caagattccg
     1441 cgccacaaaa tccggtgcac ccgaagaact gtgccgcatc acgatgcaat ccggcttcat
     1501 cgcgtccagc gtgcgcacgg tgtcgagtag cgattcacct tttttcaggc tggacgattc
     1561 cgcctgcaaa ttcatctgat ccgcgccgag ccgtgaagcc gcggtcgaga aactgatccg
     1621 cgtgcgtgtg gacggctcga aaaaagccag tgccacggtt tttccgcgca gggtcgctaa
     1681 ttatttaagt gggtgtttct gaatttcctg gaagggtttc gattggtcaa gtaaaaatag
     1741 aatttcttcg gcgctgagcg cttcaatccc tagcatgtgg ccgaaacgaa atggcacagc
     1801 aatcctcgga atcctggccg gacctgttac aaaggccttt ggcgtcagtc aactcgttca
     1861 acaagcatga cgcgctcatc ctgatcaatt tcattgaggc gtacttcgac aatttctgaa
     1921 tcgctggtct gcacgttgcg accgacaaag gtcgcttcga tcggcaactc gcgatgtccg
     1981 cgatcgatca gcacgcatag ccttacgcgc tttgggcgcc ccaggtcgaa gagtccattc
     2041 atcgctgcgc gaatcgttcg gcccgtgtac agcacgtcgt ccacgaccac cagatcttta
     2101 tcatccacag cgaatggaat atcgctcgat tgcaagacag gctgcgcggc gatcttggaa
     2161 aggtcgtctc gataaagcgt aatgtccagt gtccccacgg gtactccgac cccgtcaata
     2221 ccgcgcatcg aatgcgcaat gcgctgcgat agtggaacgc cgcgccggcg tatgccgatc
     2281 agtgccaggt tggaagtgcc accgcttttt tccacgatct cggctgccaa acgctgaatc
     2341 gtgcgatcga tttccgtcgc cgtcatcaat tggttttttt caacgattcg cattgggcga
     2401 acatcctagc atacaggggc cacgtctcga agcgcgtctt gaaccatgag caacaatttc
     2461 cgctgttttt ccacaagcct tccacaccgg cttgtgattc cgcatgctag aatttgcggc
     2521 agtgactgcc cccaacagcc caaacaccgt cgaactctcc gcgaatgtcg gcgccttgcg
     2581 cctttccaat cccatcatcg ccgccagcgg cacgtttggt tacggcatcg aattcgccca
     2641 ccttgtcgat ttaaaccgcc tcggcgggat cgtcgtaaaa ggcctttccc tcgaacccat
     2701 ggccggcgct ccggctccac gcttgtgcga aacggcttca ggaatgatga acgccattgg
     2761 cctgcaaaat atcggagtgc gcgccttcgt aaaagaaaag ctccctgaac ttcgccggtt
     2821 ccagactgcc gtcatcgcca atgtgttcgg ctgcacagtg gaagattacg tgagcgtgat
     2881 tcgggtcctt gaggaggctg atggcttagc tgcctacgaa ctgaatattt catgccccaa
     2941 caccgctcac gggggactgc agtttgggag cgatccccaa atggtcttgg aagtggtgtc
     3001 cgctgctcgc caggcagccc accgacccct atgggttaag ctttctccca acgtgacgga
     3061 tatcagtgtg attgccaaag ctgccgaggg cgctggagcc gatgctctga ccgtggccaa
     3121 tacctaccaa gccatgagta ttgacgttca gacgcggaag tcgaaattgg ggcggatcac
     3181 cggtggccta tccggccccg ctatcaagcc gattaccctc cgcctcgtct atgaagctaa
     3241 gcgctcagtc aagattccag tcgtaggcct aggcgggatt gagaacgcaa acgatgtcct
     3301 cgaatatatg atcgcgggag cgtcggcggt cgaaattggg acggcaagct tttccaatcc
     3361 tgcagcgtgt ttaaacatcc tagatcagct gaggaaaacc tgtatcaact ataagatcaa
     3421 tgatatcaat ggtttaattg ggtcatttca ggacgattct tgttgaacag gctgagatcc
     3481 catgttagca ttttaccgtg cctatgaccc ggggtaaaca atatgcgtag gcgacttttc
     3541 atgatttggg cagcgctccg ttggttgccg ctcatgttcc tgatcacgca agggctggaa
     3601 gcgcccttgg ttgcgcatgt aagccagccc ctaaagccca ttcgagtgtg gaccgcaaaa
     3661 accagttggt atggcgggaa atttcaaggc cgccgaacag ccagcggcga aaaatacgat
     3721 atgtatgccg caactggcgc cagcccgaat cttccctttg gctctttggt ccgcgtttca
     3781 aatcccaaga cccacatggc gcaattgatt cgtatcaacg accgcggtcc ttttatcgat
     3841 gatcgtgacc ttgatgtttc ttatgtggta gcatgccgtt tgggaatcct cgatagcggg
     3901 aatacgccct tgcgagtcga actcctagag gtgccaaaac gtcgctgagt gaccgtgccc
     3961 gtcagaaact gatacttgcc cttgattatt cctcacttga tcctgcttta gaaatcgccc
     4021 aaatcttatc ttctgtggtt ggtatcttta agataaacat acacttattt acgactgaag
     4081 gccccattgc cgttcagaag ctgaaccagc ttgggccagg aatctttctg gatctgaaat
     4141 tccacgatat acctaacacc gtcaaagggg ctgtgacgag cgcactagcc ttgccgggtg
     4201 tgcaactgtt ggacgtccat gctttaggcg gtttcgagat gatgcgcgcg gcggcgaagg
     4261 tcagggacga agctaaagtc tccaagagtg ccacgccgaa gctgctggca atcacaatct
     4321 tgacgagtat ggataatgct gcgctccgag gcgtcggaat atcgtcacct gcgtcgcgac
     4381 gggtggttca gttggctcgc ctggcaaaaa aggctggaat ggatggcgtc gtggcgtcac
     4441 ctcaagaagt tcgggccatt cgaaaagcct gcggaaagga tttcctgatc gtggttcctg
     4501 gtattcgacc cgttgtgggt ggcggaccga caagcaaaag ccggcacaag acagatgacc
     4561 aggcgcgcgt cgccacgcct gcggaagcga ttcgcgccgg agcggattac cttgtgatcg
     4621 gcagaccgat tacgggagcg ccggacccgg aagcggcagc acaggcaatt ctcgaagaaa
     4681 ttggttcggc cctgacttaa ggcaggcgtg agcaatttag cgtagtccgc tacccgacaa
     4741 aggaatcaga ctatcccttt gaattctgac ttgcggcaac caggctgtga tcgtgctata
     4801 attcttgcat cgagtcatgt agcaaatcac gtcttctcgg cgtaaagcga ctcgaaaggc
     4861 aggtacatca tgggagagtt cagcccattt cattggctga tagtgttggc cattgtggtg
     4921 cttttgtttg gcggtaagaa gattccagaa gtcatgaagg gcctgggtga aggcatccgc
     4981 agctttaagg aaggtatgca cggcgcgggt caaggacccg tgcaaacaac acaggtgtct
     5041 cctccccctg cgccaaccgt gacaactgtg actgaggaaa agaagtaatt cctcgagtgg
     5101 tgtgatcgaa aaaacattcg tgtgataggg cggcctactg gaccgccctt tttatttgcc
     5161 tcactgcttt tcgttttgcc aacccagttc ttgaagctgc gcataaagtc tcgccagtgg
     5221 caggcctacg acattgaaat aacaaccctc aattctggga ataaatttgc ccgccaggcc
     5281 ttgaatcgca tagccgccgg cttttccgaa tggctcttta ctggacgcgt aaacaacaat
     5341 ttcgttttcg ctcaacggag aaaacgtcac cgtcgaaatt tcgactgctg cgcgcgtggc
     5401 actatctggc aggcgcaaaa ggaaaattcc ggtgagaacc cgatgcgtgc gcccgctcag
     5461 cctgccgagt atttcacgtg cgtgaacgga atcgtctggc ttaccgataa tttctccgtc
     5521 gagctcaacc gtggtgtctg cgccaatcac gatgcactcg ccttcgccgt tgctcacacg
     5581 tgatgcggct gcacgtgctt tttcttccgc gaggcgcgca accatcgcat gagccgactc
     5641 gtgcggaagc ggcgtctcgt taatttcagc tgcgcatacc ttaaacggaa ttcccgcatc
     5701 gcgcaaaatt tcagcccgcc gcggcgaaga agaagcaaga atgattttca cagtgttgta
     5761 gcccgcctct tcagaggcgg ggattttctc tcagcacggc aaaaacttgg cctctgaaga
     5821 ggcgggctac aaaatgcaca acgcaatctc acaagtgcaa aggcagcatt tacaaaacta
     5881 cggattccgt gtactcgccg aaaacgcggc gcattgcctc ggaaatttct ccgaccgtgg
     5941 catacgcttc gacggcagcg gcaatcgcgg gcatcagatt ttctccgctg cgggcgcggc
     6001 gctcgacttc ggtcaccgct gcattcgcgc gtgaagagtc gcgcctcgcg cgcaatgcct
     6061 ggacgcgttc gacttgcgcg cgttcgaggt cggaatcgac gcgcagggtc ggaatgggtg
     6121 tttctttttg cgcgacgaat tgatttacgc cgacgacgat ctgttctttg cgctcgatcg
     6181 agcgctggta ttcgtacgcg gctttctgaa tttcaccctg cacgtatccg gtttctatgc
     6241 cgcgcaacac gccgccaagc gcgtcgatcc tcgtaatata ttctttcgcg cgcgcttcaa
     6301 tttcgtcggt taatttctcg atggcgtagg aaccgccaac gggatccgcc gcgtttgcaa
     6361 ctccggtttc atgcgcgatg atctgctgtg tgcgcaaggc gaggagcgcg gcatcttccg
     6421 tgggaagcgc tagcgcttcg tccatcgcgt tcgtgtgcag cgattggcac ccgcccagca
     6481 cagcggcgag cgcctgaatc gcgacgcgga caatattatt ttccggttgc tgcgcggtta
     6541 gcgaacttcc agccgtttgc gcatgaaagc gcagcatcat ggatgccgga agctttgcgc
     6601 cgaagcggtc ccgcatcaag tgcgcccaga gcctgcgcgc agcgcggtac ttggcgattt
     6661 cttcgagcag gtcgttatgc gcattaaaga aaaatgaaag ccgcggggca aattcatcca
     6721 cggccaggcc cacggcgatc gcggcgtcga cataagcgat ggcatcggca atcgtgaaag
     6781 caatttcctg cgctgcggtc gaacctgctt cccgaatgtg atatccggaa atcgaaatcg
     6841 tgttccacgc aggcaattcc gtgcggcacc aggcgaatag atcggtcacg attcgcatcg
     6901 agggacgcgg cggatagatg tatgtgccgc gcgcgataaa ttctttcaaa atatcgttct
     6961 gcaccgtgcc ggaaagtttc tgcaaatccg cgccctgttt tttcgcgaca gcggcataca
     7021 gcgcgagcag aatggcggca gtggaattaa tggtcatgga cgtggacacg cgatcgagtg
     7081 gaatattatt gaacagcgtt tccatgtctt cgagcgaatc aatcgccacg ccaactttgc
     7141 ccacttcacc cgcggcgcgc gcgtgatctg aatcaagtcc aatttgcgtg ggcaaatcaa
     7201 aagcaaccga caatcccgtt tgtccttttg caagcaggta acgaaatcgt tcgttcgatt
     7261 gtgcggccgt accaaatccg gcgtactgcc gcatggtcca caatcggccg cggtacatgg
     7321 ttggataaat tccgcgggtg taaggaaatt ctccggggac gcctatcaaa tcttcggggc
     7381 tgcctggtgg aagatcgtct tccgtgtaga cagtttttaa aggaatgcca ccgagagttg
     7441 tgaactgctc cttgcgctca ctcgtcctgg cttttttttt ggctgtttcg ttagacgtag
     7501 gattcatcgt acacgtcgcg aatcgcgaaa ggacaagatt ccgaagcaaa tcattaccag
     7561 cgcataagcg ccacacgtga caaggagcca gcggcccgcc ccgttgtgcc atgcgcgcca
     7621 acaggcctgc agcgcggcaa aagcgagtat gaaaaacaca gcgcccgtca cttcgtgaaa
     7681 caattcgcgc gccgctcgcc agagcacgcg gaatgtgtgc cgcatcgtcg caaaaagtcc
     7741 cataggttga gtagccacaa tgtaattcta gtacaaaacc gggtccgcga cagccgcaac
     7801 ggtgagcata ggcggattgg gcacctccgc catatcctcg gggcttgtat cccgagtcac
     7861 agcgacctac aattaatgcg ggggaagttt ttccaaaggg aggcatcgtg caactggatg
     7921 atcatctaaa acaactgctg cgcgagttgg ggcacgcgat caatgatacc gtgtcggagt
     7981 ccggtcgtat ttcggaggct atttctgatg tccggtccgc tggattcgac atcgtgctga
     8041 agctcgacgc cacgattggt ctggcacgac gcgatgggga cgataccaag atcacgacgg
     8101 gcgaccggcg ttttctggaa tctctgcaca ttcaagtcga ccaggaaatt ttgaatgaag
     8161 caacggaaga gcaagagtcc cggaaaatgg aaatgacgcc gcaagatgtg cgttttttga
     8221 agtcgctaaa aattaccgcg gatgaaacgc aacagcagta agaggcacgc aattttcgtg
     8281 tctgatttga tcgcgctgta cagcgcttcg cgataagttt tggttccgga agtacccact
     8341 gtatcggatt gtcctagcat gaacactctc catgattcgc cctcctgctg tcgctggccg
     8401 cttttatcct gatgatccgg cgcgtttgcg cgccgaaatc gattctttcg ttttccccag
     8461 catcaaggaa aagaaaattc ccgcacgcgc gtgcattgtt ccccatgccg gatatgtgta
     8521 ttcgggcagc gttgcggggg aagtctacgg ccgcatagac attccaaagc gtgtcatatt
     8581 gctcggcccg cgccattatc cgcacggcgc ttcactggcc attaattctg gcgatgcgtg
     8641 gcagacgccg ttggggatgg tggcggtagg tcatctcctt gcggaaaaaa ttgtccgcgc
     8701 atttccggaa ttgcgagaag atgacgtcgc ccaccgtacc gagcactcgc ttgaggtgca
     8761 gctgccgttt ctgcaacggc ttgtcccatt cttgtcgttt gttcccatcg tgattggccc
     8821 ggcgcaatgg agcacgatgg ataggcttgg ccgtgctctc gcaaccgtga ttgccacgga
     8881 gagcgaaccg attttgctca ttgccagctc ggacatgaat cattatgaaa gcgacgccgt
     8941 aacgcgcgtg aaagatcgca aagccattga gcagattctg gcgctggact ctcgcaaact
     9001 tttcgatacg gtgcgcgagg aacaaatctc gatgtgcgga tatgccgccg cggttgccgt
     9061 gatcgttgcc gcaaaagaac ttggcgccat tggcgccgag ctgattcgct acgcgacctc
     9121 aggtgaagtg aatggcgata tgcaggaagt tgtgggatac gcgggaattg tgatccggtg
     9181 aaggcagttc actcttgatg ttttttcata gctttacttg tctcggcctt gctctcagtt
     9241 aaaagaaatg gcgcgggttt cagcctgcgg gtttttggcc ttgcaaggcc taaccccaca
     9301 ggcggaagcc catgccacaa aatccaaatc aatggtcgcg gggggcgcgg gaatttctaa
     9361 tgcgctcggc taaggcgcgg gcgcgagtgc gggcgatttc cacaaaatgc ggatctgcgg
     9421 cgatctgatc gagcaccggc aaaacgcgtt caggctcagg caggagccga tgttccatgt
     9481 ccgattcaaa ttgcatcagc gcgtcgttca ctcccaaacg atcaaactga gcgcgtcgct
     9541 gcaagagcgt caaatcctgc tgctgtttgg ccagcccttc aaaaatctgc atcagttgag
     9601 ttgccgatgc attcatcgtg tagttgaacg atgtctcgtg cgcttccgcg ccgttttcgt
     9661 aacgaaatgt tttttgtccg aggttggcaa tttttttttg aatgtcgaga ttttgattcg
     9721 caaagtgatt caggtccgag gccaggtcga atattttctt ttgcacgtcc ggccccacct
     9781 cgaatgtttg gggatcggcg tcttcatcca gttggcgaat atcgaatgta catttgccct
     9841 gatcgctgat ttttacttct atgaattcag gagtgctgcc cttaaagacg cggcggtagg
     9901 tgattgtggt tcccgctgga ttgccatgca cggcaggaat aaacaaccat gcgccagcaa
     9961 gaaggcacag tagtcgacgt agaaaggaat ttcgggtcat cgtgaagata cgatgcgcgg
    10021 ggcggcgccg cggctgtttg cgtttccaag agattccggc tgctcgcgtt ccggcccggt
    10081 tcgtgcagat ccagtactaa gccgcgaacg aagccgttcg gcttgcacgt ggcacaaggc
    10141 gacggccagc gcgtcagccg cgtcggcggg ttcgggtgtt tcgctcatgc cgagcagcgc
    10201 acgcaccatc aattgcattt gctctttagc cgcattgccg taacccgcta cagaagcctt
    10261 gatttcgcga ggtgaatagc tgctgacagg tatgccgcct tgggcagcgg caagcaaaac
    10321 cacgccgcga acttctgcca gcctcaaagc ggttttaata ttcagggcgg tgaatacggc
    10381 ttcgaccgcg actccatgcg gctgaaattc ctcaatcaac ccggcgagca tggcgtgaat
    10441 ttctcgaagg cgttccggga aatggtccgg gcctttgcgt gtggcgcgca acgcgccaaa
    10501 gcgcagcatg cggtaacggc ggccgtcgct ttcgattaca ccgtaacctg tggcgcccgc
    10561 agcggcggga tcgacgccca gcacacggaa gaattcaaca tttgttggat atgcgattgg
    10621 cacggctttg agtttacggc ggcgcgccgc gtccggccac aattttttca gcgggatgta
    10681 gcaaaaacga attcgtgcag gttagagcaa ggttgaaaca ttgctgctct aggccgcctt
    10741 ttcagagatc gacatttaga tttcgcttcg tctaaacccc cgcttctgaa gacgcgggct
    10801 acagcaatag atgggtcgac agacttattc agcagccatg acggcttgca tttctttctc
    10861 gtcgatgtcg aagttagcgg aaactttctg aacgtcatcg tgttcttcga gcgcctcgat
    10921 gagccgcaac atctgcgccg cctgcgtgcc ggcgagcttc acataatttt gtggtaccca
    10981 gccgacttct gccgattcgg ttttcacgtt agccttagcc aaggcatcgc gtacggcttc
    11041 gaagcgttcg ggcggagtca ggacttccca cgcctcgcca tcgtcgcgga gatcctcggc
    11101 accggcatcg agaacgatgg acaacatttt gtcctcatcg gcctgttcct tggatacaac
    11161 aagagcgccc ttgcggctga acatccagcc cacggctccg ttttcagcca tgtttccgcc
    11221 atttttgttc atcatgtgcc gaagttcgct gacaacgcga ttgcggtttg tgctgaccac
    11281 ttcaatgatc atgcctacgc cgccgggacc gtaaccctcg aacgttactt cttcatactg
    11341 ttcgccttcg agcgtcccgg tgccacgcgc cagggcccgc tcaatatttt cattcggcat
    11401 gttttcattt ttagccgcca ggatggcagt gcgcaggcgc gggttggagt ccgggtcgcc
    11461 cccacctacg cgagcagcta tggtgatttc gcgaatcact ttggtaaaga tacggccgcg
    11521 cttagcgtca gtagccgcct tcttatgttt aattgttgcc cacttagaat gcccagacat
    11581 ggtaatcgcc tcttgggaaa tatgttgggg gactgatccc tgcttattgc cacgacagtt
    11641 tagcatagcc cttatcgaat cggaacaaga gcaaagattt tcagaagagg cgtatttttg
    11701 gtgaaagttt taaatctccc ggcatcctta agatgcaaga gactttacaa acttcatttt
    11761 aggatgggat taacttcgat cttgacttct gacgaggtaa tcgccggttg tccgggctcg
    11821 atgggctgat tcatgtacac ggctttgcct cctgtatccg caaactccca aacggagttg
    11881 ggcggcaagc ggtcgcgaac ctcttccaaa tgttcatcgg gcacaaccag aaacacacgc
    11941 gatgggccaa cccataagcg ggaaaagtcc ttatcgtcaa aaaaccgttt cggagcgtcc
    12001 aggtatttgg agccaaatct cagattgctt tccgccgcgt cgtagagcat tacgtcacga
    12061 tgagagtaaa acgcgatccc gggagcgacc cgaatatcgc caaatagcgc gatctggtcg
    12121 tcaggctgca aaactttatt caactcaagc gcgagcgctc gcgaagagag agtagggctg
    12181 aggacaccgt aggcgatgtt ggcggcaatg aagaaaccga ccatgccggc agccaaggcg
    12241 aaatttggat accaggaaat tccacgctcc cgccaaagcc atgccaacac aaaaggaatg
    12301 agaatggaca gtgaggaaat gagaagggga attcggagat cggagaccga ttgcggagtc
    12361 aagtccagca catgcgccat gcttgtcatg tatttttccg ggctgcgcaa ggcaagatgg
    12421 ctggaaacat cggtggccgc ctgaattttc agcgtccata ttatcaagta gcccgaaatt
    12481 gcggcaaaca ctacagccac ccccgcgagc actcgctgga tcggcttcaa ccagaattga
    12541 tccgactctt ccgcctgtgc aattccatag cctagtaaca aggcaatcgc cggccatgct
    12601 ccgaagctgt agtactccat gcgtgatccg ctttccaaag taaaaaatcc cagaatcgcg
    12661 gcggcccaca caaatagcag taatcgcgcc tgccgcttcg gagtattggc ttcacgccag
    12721 gaactgggca atagcggagc gttgcgaagt gcgagtggga agaaaaagct ccagggaaat
    12781 aaccagatca ggtgcgaact ccaccagatg ccgagcggca ctgctccgta atcgtgagga
    12841 agccgtgttc ccaacgcgcg attaatatgt tcattaatga agtaggccca gaaaaaaccg
    12901 ggcgtgcgca acgccgccaa aatatgccac ggggcggcaa ccgctagaaa aattgccgtg
    12961 ctggaaaata ggcgcagttc gcgccagcgg tgccagcctc ccgtgatggt aataaacgtg
    13021 acgattgcga ccgcggggaa aagcaggccg atgagcgcgc gcgtcattac cgcgacgccg
    13081 cagagcgcag cagcgcccca atacccaatg cgagaatcga ggcagccgga ccaggacctg
    13141 agaaataaat agaaaattcc ctcgaacgcc agagcgtaaa ttgcttccgg aatcatgacc
    13201 cgcgtaaaaa tgaacatgcc ggcgcttgtg gctacagcca atgccgcgta cagtcccgcg
    13261 cgctggccaa aaaatctgca gccgaattca aacgtcagca acaccatgcc gatcatggcg
    13321 agggccagcg gcaggcgcgt cgcaaattca ttttcgccga acaacttata gctggcggca
    13381 accagccagt aatgcatggg ggggcgaatc aggtagcgga ttccatccat ataaggaaca
    13441 acaaaatcat ggcgctgcaa catttcttgc gagaccattg catgagccgc gtccacatca
    13501 tcgtcatcca gggccggcgt tccggcggtg ccaatataaa gcccacagga aagaagcagt
    13561 agaaagacca gtatccacct tgaccgaaca tgaaaacttc gcatcaactc atcctcacgc
    13621 aaatttaaaa aaaacgatac gtaaatttac gagtgggccg attggaatga tttgtcaggt
    13681 tggaagtgct ccacactaca cgtatgcggg taccaattcc tcgatcctta caggaatccg
    13741 acgtcagata cattagcgca tgcagggatt gtgtttgacg agtatatttc tattttggat
    13801 tgcggttctt tcatactatg cccatttctc gcgataattg ttctgcctcc tgaatcagag
    13861 ctccgaggct gaccgtaatg ccgtcgttcc agaacgcctc attccgcgca tccagtccga
    13921 acggcgcaag taattccaca acgtccttgg tggacccaga gcgcagtaag tccagatata
    13981 atggctcaaa ttttgcgccc aacttttcct tttgcgcgaa aaggctttgc gtcaggagtt
    14041 cgccgaaagc atagccatag acgtaaaacg ggctgtggaa gtggggaatg taggcccaca
    14101 aatgttccga gttctcatac gtaaaaatat cgccgtcctt gccgtagagt tcctgcgccg
    14161 ttttcagcca aatcgcgtcc aactcttcca cggatcgtct ttttggttcg gaccaggtcg
    14221 aataagtttc atccatgccg tgcaagcggc gctcaaagtt tgagaagccg atctggcgga
    14281 cgacggtgtt gatcgtacta tcaatcttgg ccatgatcag cgcaagcagg gaacttttgt
    14341 ctcccgaatg catcaggcgc tctttcaaga aagtaaacgt ggtcatttcg ccgaagacgg
    14401 atgccgtttc gcaataggcg atgggggcat ggaacatcag cgggccttgc gcttctcccg
    14461 cgaggatgcc atgcacgccg tgtccaagtt cgtgcgcgag ggtcatcacg tcgcggttgg
    14521 agcccaaata attcaatagc gtgaaggata ccgcttttcc gccgggcagg acatgcgagg
    14581 aattgaaagc tccactgcgc ttttctttca ccactggcac atcgatgcgg cgttcgacaa
    14641 aaaaagtttt tacgagagcc gtcaaagtcg ggctgaaact ttcgtacgat ttttttacga
    14701 ttcccgcggc ttcatcgaaa gggattctgg tcgtgtccgc aaacggcatg ggcgcattgc
    14761 gatcgctcca gcgcatcacg gtcaatccca gatggcgcgc ctttaacttg tagtaacgcc
    14821 gcgtcaaggt gccacccagg ttcgttacgg cattatgcag aacgttgacg acagagtccg
    14881 ggatgcggtt ggacttattt cgcccatcca tcgggtgcgc gtatccgcgt tctttttcct
    14941 ctactgcttt taatcctgca accatgtaca gcgtttgtgc ggaatatttc gcgaatggtc
    15001 ctgccatgcc gctgttcacg cattgcagag cttccgctcg ttcgttggaa tctttcgatt
    15061 cggcgatcag gtgcatcatt tcggtcaggt ttttagtgcc ttcttgaaat tcaaaattca
    15121 ggtcggcttc cacttcatcg aaaaaattgc tccaggaact cgaattgaag ggggagcgct
    15181 tgaccagggc ggactcgacc gcctcactca agaaatgccg tttaaaggtg cggatgtgtt
    15241 cgatccacgg gcggtgtttg gaaacgagcg agtcgctggc ataaaattta ttcagcgttt
    15301 cgtcgctcag ttgcacgagt tccaattcaa aaaatgtcag gtgctctccc tgcaccgcgc
    15361 tcatctcgcg ctcaaatgcg gcgtgcttgg ccttgatcgc cgcgttggtc aggtccgtgc
    15421 tctctctcaa gaagagatac gaattcattt tgccgctgag catttcgatt tccgcatagt
    15481 ccctgattgc agcgcccagt tgctccgcga gcttcccttt gtaggagtcg gagaaatgtt
    15541 tcgccatggc cttcagttcc tgtaaatccg cgtccaggcg cgggtccgca atatccgtat
    15601 acaggatgct caaatcccag cgaatatcat tggcgctttc tactgtgttt tccatgatgc
    15661 cctggtctcc tgaattcatt tgcgctgtga atctggcgta attatttccg tttacggcgt
    15721 gtcgatcgcc gttacaaagc ttccatcact tcggtattgt accccggcgc cgttgctcaa
    15781 ttattatttc ccgcactgca taaagtacag gatggcggcc ggaagtacag atgtctgatt
    15841 caccgcaatc ctgtatcgag atgtggattt ttagggcgga tgacgcttac aatcgttact
    15901 tcggagtatt gtctccatca atgcctacgg tagttgcatg cagcgaaaaa ggttgcaagg
    15961 gacgggtgcc tgccgcgctt gaaccagtgc actcgtgcgt cctccatttt acgctcaaaa
    16021 tcgaaagcga atgcgcgcaa atgcgccgcg aaactgtcta tggacggacc ccgcacgccc
    16081 gccaggtgga aattattaat ttcatcgctg acaacggtga aatgctcgcc cgtgcctcga
    16141 ccagcggcct gcacctcgcc gatgaaatga aggctcgaat cctgaacacc ttcctcactc
    16201 taatgaacct gcgcgaaaat ctggaccgtg ccgccctgcg ccaacccatc ggtcgaacca
    16261 gcggatagcg ctccacacat cctctgctga acgtgtaaga gactcttccc gtattaaaat
    16321 agcttgtacc ggcaatgcct ccgcaaaata aaaaccgcaa aaaaaacgac cgaataattc
    16381 ttcaacctat tgaattgcgc tggaaacctg aaggcacgta tgattcaaaa tgaaaatcca
    16441 ctctgccact gccaaacggc tggccttctc tcgatcgata gctgcatact gtattcggtt
    16501 gaactaattc catgaagcgc cattcgagtt tgcatccgct tagccaacac caccattttg
    16561 cgctgattca ggcgctggag atgcgccgag ccgcggaaat gccgctggag acgcgcgccg
    16621 ctcatataga aaaacacgcc gcaaaatttg tccgcttctg gcataaaacg gggctcgtgc
    16681 atttccgcga agaggaagag atcctgttac ccgcgtattc ccggcacacg aggctcgacc
    16741 gggacgcgga cgtcatgcga atcctcgccg atcacgcaga aattcgcgct gccgtgctgg
    16801 atttcgagca gcgccttgcg gcaaaaattc cggttgaagc ggaacaaatg gcgcatctcg
    16861 ccaaacttct tcacgatcat gtccgctttg aagaaaatgt gttgtttccg cgaatagaaa
    16921 aaacgctggg cgaagaaggt ttgaataaaa tgggccgcgg attaactcgc ctgcattcca
    16981 agaatgatcc ctgcgagatt tgagtggaat aggcttgaga gtctgggtga gaactgccga
    17041 atcgttgtgg gtcgtggctt tagccacgac ataagtccta caaaataagc cggctttagc
    17101 cgctgaagtt ccaatgtcgc agtttttacg aagactcttc agcctgtcct actaaacccc
    17161 aatgtagcgc gcgtagaggg agcgggcgat gctggaatcc tgcgtgcctt taataatggc
    17221 acggccatca gcaaagaccg tcatttcata aggcggaacg cggaagcgca gtagaaaacc
    17281 gttttcgcgc acttcggcga cggtgggggc aatgcgtttt cctaaggcag acaggtccag
    17341 ctggcggcgg cgttcgtgaa tctgcacgga atcgcgcccg cacatcgtca agtgaggctg
    17401 cgtttcgcct tccagatagc ggaaatcgtg gcgaacgcag gcgcggcaat ccgtattgcg
    17461 cgccacgcgc acggattgaa attttccgga ccagacatcg aatgaattca gccgcccgcc
    17521 ggttagttcc ggttttcccg tgagcaattt aatcgcttcg gcggcttcga tcgaagcaat
    17581 cactcccgcg gctgcgccaa gcacgccaat cgtgtcgcaa gtatcttccg accccattga
    17641 ctgtgagccg ttcgcgcttt cggattcgag cgcgcacgca aggcatgccg tctctccggg
    17701 ccgaaccgtg agcgttacgc catagctcgc gaccacggcg gcgtaaatcc aagggacatg
    17761 taagtgaatg gcaacatcat tcagaaggaa tcgcgtttca aaattgtctg tcccatccag
    17821 aatgagcggg aagctgctca aaaattcctc agcgttttta gagctcacat cggcaacgat
    17881 gccctcgacc accacgccag aattgatggc ccgaaggcgg cgctcagcgg caacagcttt
    17941 aggcaacgct tcctgcgcat cggattcttc aaataaagtt tgccgctgca aattggaagg
    18001 ctccacaaag tcgcggtcca caattcgcag cttgcccact cccgcgcgca cgagcagatt
    18061 tgcaagcgcc gtacccagcg cgccgcatcc caccatcacg gcggaagatt caagcaggcg
    18121 cgcttggccg gcctcgccaa tttcgctgaa aagaatttgc cgcgaatacc gatcgtaagt
    18181 ttttgcatcc atcgaaccga gcgcttctct cattcccggg ccagtaaaaa caagcatcag
    18241 cccgtctgct tttgaattac tttaaaataa tagaaaatca aacccggaca ggtcgaaaac
    18301 ctatcctact cagatgccat catagcttgc gagcgtcgcg catggaaatc atgtgtaggt
    18361 ttccgcgagc cgcgctgaat tcgcgccaaa tcgccggctt cggctatgct accctagctc
    18421 tgttgtgaag agagatgcca atcgttcact gcccatttat acgcttcgcc gcatggctgg
    18481 cgacgctctg gttgctctgc gcgagccctc tcgcggcgca gaacaatgtt gcgggtgatc
    18541 cgccacgcgt ggtcgcagtg cgcgtggtca ctgaatccgg cgcagttctg gaagaaaatc
    18601 cccctaaact gacgcttcag cccggccaac ctttttccac ggaatcggaa agcgtgagcc
    18661 ttcgtgagct ctttcgctcg ggaaaatatg cggacttgcg cgctgagttg gcggatgtcc
    18721 ccggaggcgt tcggctggat tttgtcgtgc gccaaaatct ttacattaat cgagtgcaaa
    18781 tcgatggctt gcacgagcct cccggcgaag cgctggcact ttccgcgctg cgtcttaatg
    18841 tgggagagcc tttccgcata agcgacatga aggatgcgct ggaccgcctc cgccaaacgc
    18901 ttgaagatga cggggagtac caatccaaac tgacttacat cacaacgcca catcccgaaa
    18961 cactccagat ggatattttg gttcacgtta tggaaggtcc acgggcgcgc gtcggcgcaa
    19021 taaccattca aaatcaaacg gaatttccgg agcaggagct ccgcaaccaa ctgaaaatcg
    19081 gcgagggaag ccaaattacc gtcgagcgtt tgaatcgcgt ggcagaccgc gcgcgaaaat
    19141 ggctgacggg aaataattat cttggagcgc gggtcaccat tcatcgcggc acatatgacg
    19201 cgacgacgaa tcgagtgccg ctgaatttcg cgatgtacgc tggtttggaa gtgcgcgtgg
    19261 ctgtggaagg cgctcgaatt tcctcgggaa atttgcacaa gctgattccc atttatcaag
    19321 aaggcgcggt agaccaggat ttgcttcagg aaggccgccg ttcgttgcgt gactggtttg
    19381 agcgagccgg atattttgac acgcaagtta attacaccgt gaaggaagcc cccaccggga
    19441 aatccagaaa cacaactcat cgcaccgcaa gtgttgtaac ctacgatgta aatcgcggcg
    19501 atcatcaccg gctggtcaaa gtggaattcg acgggaacaa atatttcagt tccgatcttc
    19561 tccggggacg cctgaagatt tttcccgcgg gttatgcttc accgggacat tattccactg
    19621 aggttctcca ggacgatgtc gcttccattc gcactttgta cgaggcaaac ggtttcgaac
    19681 aatgtcaggt gcagagccag cttgtagata actaccgggg gcataacggt gacctctccg
    19741 tgacgtttga ggttcaggag ggcctgcaaa ctcgagtggc gggtctgacc atcgaaggta
    19801 acgcacaact gagcctggac gagcttctgg gcgtggtagg ttcctccaaa gggcagccgt
    19861 attcagaatt taacgtttcg tccgaccggg acaacatact cgccacgtac tacgaccagg
    19921 gtttttcgga agcgcagttt actgccaatg tcgagaaaat tcctccagca gcgggaccga
    19981 atgccggccc tacagttcgc ctgacctatc acatcacgga ggggcggcaa gttctggttg
    20041 cgcgcgtcct gctgagtgga tacgaacata ctcgccctgg cgtaatttcg cgcgaagtgg
    20101 gaatcaaagc gggcgagccg ctgagtgaag gcgcggttgt cgagacgcag cgcaaacttt
    20161 acaacctggg aattttcagt cgagtttcca ttgcgccgca aaatgccgag ggcctggacg
    20221 aaaaaaagac cattgtcgtg atggtggacg aagccaagcg ttacaccatt gcatatggcc
    20281 ttggattcga agcgcagcga ttaggaagtt cgacgaacgc cgcggaccaa tcgcttaact
    20341 ttgctccgcg cggcactctc gaattgacta aagcgaattt aaccgggcgc gccgattcgc
    20401 tttcgtttaa agcgcgggca agcaccattc aaggccgcgc tctgctcact tatatagcgc
    20461 caaattattt cgcatccccc gatttcagct tgcagctttc cacgttttac gaaacatcgc
    20521 gcgacgtgca aacattcgat tcccggcgcg cggaagcttc cgctggactg gcggaaaaac
    20581 tttctccctc aagcacgatt ctttaccggt acgtttatcg tcacgtggtt gccagcaatc
    20641 tgaaaatcgc cgttgaagaa attccgttgt tcagccagag cacggaagtt tctgaattcg
    20701 atgtgaattg gctgcgagac cgccgcaaca gcccgtctga cccaagccgc ggcgatttcg
    20761 aaaatgtcga catcagtctg gcagcgaaag ccattggctc ttccgcgaac ttcatccggg
    20821 tgtacatgca gaattcgacc tatcgcccca tcgggcggcg gctagtcttc gcgcgttcca
    20881 ctcgttttgg aattcagacc gtttacggaa actccctttc taccgatata ccgttacccg
    20941 aacgattctt cgccggcggc ggcactacca tacgcggatt cggcctgaac caggctggcc
    21001 cccgcgaccc tttcacaggt ttcccgatcg gcggacaagc ggagcttatt tttaatcagg
    21061 atctgcgctt tcccatgcac ttgccgctga ttggcgaccg gctgggcggt gcggtgtttt
    21121 acgacgcggg caacgttttt cccagcattc gaagaatgtc atttcgtatc gcacccccgg
    21181 ctccaacttt cgatccggct aatccgaatg tgtgtttgac gaattgcacg aacgatatga
    21241 attatttctc gcacaccgcg ggatttgagt ttcgctacag tacgcccatt ggtccagtgg
    21301 cgctcgacct ggggtatcaa ctgaaccccg cgaaatatct gattcaaagc ggcggcacgt
    21361 gcccgccctc gcaggcagca gcttgcctga caaccactac caggctcccg gcattccaat
    21421 tcttcgttaa cttggggaca acattttgat gcgccgggtg atgatcattt tcatcctgtt
    21481 gttcgcagcg cccgagacgc cgcgagcttt cgctcatgtg cctctgcctc agcgtgtggt
    21541 ggatcgcatt gtggcgcgca tcgaagacga tattattctg caaagccagg tgcgcgaact
    21601 cggcgcattt caacaattga tcgatggcca ggcggagagt gacgataaac tgttgcagga
    21661 attgatcgaa caatgggtgg ttgccacaga ggcgaccgct tctcattttc cgcaaccagc
    21721 ggaatttgaa gtggatcgag aaatggagcg actcgtcagc caatttgcaa atatggaaaa
    21781 gtatcaggct cgactcaggg aactggggat ttcggacaat caggtacgcc aattgttaat
    21841 ccggcagatt tatgatgaac gctatgtcga ttataaattc cggccgtccg tgcgagtgga
    21901 agcacccgac gtggaagagt attacaagaa ggatttgttg cccgagcttg ccaaaaaaaa
    21961 tcaacctacg ccggcattcg ccacggttga agagcagatt cgagaattgc tcatacaacg
    22021 cggaatcagt tccatgacat tgaagtggct ggacgacaca aaatcgcggt tgaggattga
    22081 aatcgaaacg ccgaacggca acccgtagtc gattgtctcg actgcttttc atgagcggaa
    22141 cgaagtggaa attcggattg aaacatgggc tgggactatt cgtcggcctt gcgctgatcc
    22201 tcgtcttggc gattgcgtat gtgattgcca atggattcgc ggatcagtgg gctcgccgca
    22261 cgattgtgga acagctcgaa aaagcgacag gcgctcgcgt cgagctcggg aattttcaca
    22321 tcacctggag aaaactcacg gctcgatttg atggcttgac catgcacgga cgcgaaccgg
    22381 ccgaaggccc gccgctattt cacgcggata aattagaatt cgatatcaaa atcgattctt
    22441 tctggggcag gaaaatttcg ctggctaatg tggaaatgtc ccgcttctcc gctaacattc
    22501 aggttgataa agacgggtcg acgaatattc ccgggccgaa ggttgtggtt agcaccggtg
    22561 cgccggattt gcggaacctg ttcgacctaa aaattgcgca attgcatctc gacgaaggcg
    22621 aaattatttg gaatgacaag cgaattccgc tggaagccaa aggcgggcag tttgatttcg
    22681 ccatgaatta cgttacggaa ggcggccttc cggtttatct cggccaggtg agctggcagc
    22741 agtttcaagt cgcggcttta cacaacctgc ctttcagcac gagtttcaca agccggttta
    22801 gcttgcgcca gacctcattt tccgtcacgc agttgcaatg gaaaactccc ggcggcgaaa
    22861 tcgatgcgca agcaaacctg cccagcttcg cgcagcctgc ctggacattc cgctatcgcg
    22921 gtcaggcgcg cctcgaagat ctttggacaa ttttgcggca acgaggcgct cccggcgggc
    22981 atatagattt tgttggcgag gggaactact cggccgacca aatgaatttc accgggcgct
    23041 atacggcgga ccgcgtcacc atgaaatacc agtggtttca tcccggaaac atttccgccc
    23101 atggcagcta tcgtgcaaac gataaaacaa tcgatttacc ggatctcgaa gccctgttga
    23161 ttggcggaca tgtcactagc catgtgagcg tcgatctggc gaagttggaa ttccgcgccg
    23221 aatccaaagt gcgtggactg agtttgcgcc aggcccttgc cacggaagat aaccccagcc
    23281 tgccaatcaa tccgctccat tgggacagca gcatggatgt ggatgcgacc acgacctgga
    23341 ttgccggttt caagcatgtt gtttcgaaag gatcttctgt gtggacgcct gcggtcaatc
    23401 ccgcgccggg cacgattccc acagcggcca atttcgaatt taatttcaat atggaccgca
    23461 atcaattcga actttcgccc ggcgaaataa ccacgccgac aagccgcatt caatttcgcg
    23521 gcgtcttaag catgattaat acttccctgg atatgacggt cgatacggat aatctgactt
    23581 tatgggacga tttcattaat cgtctgcgag gcgcggattc gactcctgaa ataatgaaag
    23641 gacaattcca ctgggagggc cgattgctcg gtccgctgga tggccccact ttcgccggac
    23701 acttcaaggg aatggatgcg agctatggtc cctattactg ggatgatctc gaaggagaat
    23761 tgacgtattc acccgccgaa ctgctcctgg agcgaacacg tgcgcggaga ggacaatctt
    23821 ctgccgatct tgaactggca ctcgatctgg acaactgggg cttcaccgcg gatcgccact
    23881 ggacttttga cgtgaacctg gtgcgcatcg atacggatga cctgcagaaa atgttgggca
    23941 catcctacga tgcgcgcgga gttttgaccg gacaatttca tttgaagggg acacgcgctc
    24001 aaccggaatt taacggcctg ttcgatttgt cgaacaccaa agccggatcc tggcggtttg
    24061 aacgagcgcg cgggcaattg tccatccatg ccggagaaat acgaattgcc aacgccgaag
    24121 tacgattgcc ggcgaatacc ccgggcgccg ctgctggaat tgtaactggc aatctggatt
    24181 atcatacgga ttccggagag gcgacatttg atttcacggg cgcggccatt ccccttgaat
    24241 ccattggaaa aattcaatcg gcgcggcttc ctatcgctgg ccgcctcaat gtgcaagccc
    24301 atggacaggg actgctgcgc gctccagagc ttcatgcgac cctgcgttta atcgatttaa
    24361 aacttggcaa cgatgtggtg ggaagtttcg atggcaaagc cgattcggac ggaaaacact
    24421 tgcacgccac catcgattcg gccatggcgt ccggaaaact ttccgggaaa ttggatatcg
    24481 ggctccaggg agattacccc gtaagcgcgc aagtgattgc ccaacgcatc gacttcaatc
    24541 catttctcgt ttccgcatta catctgactc gtctggaagg ttccagcttg gtcgacggtc
    24601 aatttgacgt ggccggtttt ggcggtcgcc cggaaacgct tgcggtggaa tcgaatcttt
    24661 cccgcttaac attcgatttt aggagcgtaa aactcgaaaa cgtcggcccc attcgtttga
    24721 cctaccggcg tgacgaagtg cgtatcgagc aagccaatct gcgcggaacg gatacggact
    24781 ttcatctggc tggttcggca cgattcacgc aagaccaagc cctcaatctg cgcctggatg
    24841 gtgcagtcag ccttcaatta ctctccagtt tttattcgca actggaagcc agcggccgcg
    24901 cgcaaattaa tgccgcggtt gccggaacgg tggccactcc ccgattcaat ggaaaagttc
    24961 atctggaggg cgcgtcatta cgctatggcg atttcccgac aggcctcagc aatgtgaccg
    25021 gagatcttaa ttttgatgtg aaccgcatgg tgtttgacaa cgtcgttgcc gagagtggcg
    25081 gtgggcacct gaccatcgga ggcacggttg cttacggcgg cgggccgtta aattacgtgc
    25141 tcaacgcgcg ttcggaccag attcgcattc gctatccggt cggcatgagc tggctcgtgg
    25201 gcggaacctt gcgcctttcc ggaaactcac aggccgccac actgtccgga cgcatcagtg
    25261 tcgaccgatt gctgatggct gagggattcg acatcgccag catcatggct tcgtccaagg
    25321 agccggccag cggaccaagc acgacttctt cttttctcca aaatttgcaa ttcgatgttc
    25381 aggcggaatc cagcccggat tcgcgcatgg aatggacagg agcacgcctg caaaccgatg
    25441 ccagcctgcg cctgcgcgga acgtgggaac acccgatttt gctcggccac attcacttgt
    25501 taagcggtga aatgaatttt cgcggaaatc agtacaccat ctcccgcgga gatattaatt
    25561 tcgccaatcc atttcgtctc gatcctgtca tgaacgtgga agcgacgacg acgatccgtc
    25621 agtatgaagt cactctcgat ttcacgggcc ccgcaagccg tctcacactc gcgtaccgat
    25681 ccgatccgcc gctgccatcg agtgatatta tcgaacttct cgccctgggc cagaccggcg
    25741 aagaaagcgc cgcgcgcacc gccaccgggc aaaactccag cgcaggcgcc tccacgctgc
    25801 tatccgaagc gatttccagc caacttggcg ggcgcattca acgtctgttt gggatcagcc
    25861 atttcagcgt cggtccatcc gtggccacgc tgaatacaca gcaaaactcg attgccagcg
    25921 tcaccattga acagcaggtg acacgaggct tggtgatcac ctacattacg gacgtcacca
    25981 cgacgcagta tcaagtgatt caaatcgaat atacgatcaa ccgcgaattc tccgtagtcg
    26041 ctctgcgcga tgaaaatggg acattcggtc tcgacgtggt gcgaaagacg cgttttaagt
    26101 agaagccatc ttttttggta tgtcttccaa actcgccagt gcgacagggg ctaaattcct
    26161 tattttttca aattcctgaa tgtcacggct gaagaggctg cggaaaaact caacattttg
    26221 tcattccgag cgaagcgagg aatccctcct tctttatttt cttatactta aatcgaagag
    26281 agattcctcg cttcgctcgg aatgacagaa taaacctact ttttccgcaa actgtgaagc
    26341 cgcgacccac atggaatact tatgaaccaa gcatgaacag gcacggcaca tcgctgcccg
    26401 attcccggct ttgagaataa acttcgtgct atcttcagct tcgaggagta gacatggcac
    26461 aagtctgggc gaagtatgtt aagttttttc gagtgtcgat tattgttgcc gccgctttgg
    26521 tggcagttgc aattcccgca ggtgcacaaa aaaaagataa gaacaaaact gctccaactc
    26581 ccgatccaac ggaggaactg aagaccgcca tgcggggacc tgattcgcag tttatcgatc
    26641 agaccatcgg cgaggcgctc ggatactggc aaattggcga caccgaatcc ctgcgcaaat
    26701 attatgcgga ggatgtcgtc gtggtgagcg gtgcctggga accgcccgtg atcggctggg
    26761 acaacttcct gaaagcgtat caggcgcagc gcgctcagat caacggggcg cgcatggacc
    26821 ggattaatac gctaatcaaa gtaaatggaa attccgcgtg ggccacgtat caatttacgt
    26881 atgcagcgca gatgaatggt gtcgtggttc aatttcgcgg ccacacgaca ttgtgtttgg
    26941 ctaagcaagc cgataggtgg gtaattacac taaaccattc gtcgattgtg gactcaactg
    27001 cccctcagcc cacaacctcg accggtccgc ctcaaactac aggtaaaccc tagaaactca
    27061 tgaaccccga ataaatccag actcccgcgc tcgccaacag aagcgctccg gataaaacgg
    27121 agagcgcgat gatcggcttg gtcaagcgcg acatttgagc aatgatcgcg atctgctcct
    27181 ggtaatgatg ctgctctgcc gcatcgatga agatctgatc atcttctctc gacgaaagtg
    27241 tcgcccggta cacaaccaat gcgattagaa caacagtgat tacaatccag gaaatcatga
    27301 gctgattgag aagtaccgta cgggtcagtt ccatgagcac ctcctgatga gaacggcact
    27361 gggcggaaac gtggatgcgc cattctacag agacaaaacg tgcgcgaagt atatcactat
    27421 tcctggggtt ttttgcggag gctgttacct atcaggcatc agcctttggc ggaaatcgca
    27481 cgccccagaa gtcgtcgagc ggctgcaagg attcgacgcc cgtttcccag agttcagtgc
    27541 cttgcggctc cttcacccac gcaacacgga acttcgcggt gcgctgcgag aaacggttgg
    27601 taagttccac ttcagcgccc gtttgcaacg ctttcgatgt ccagatcata gcgccaagca
    27661 cgcctacaac gccggtgtaa gtcgactcct caatggcagt tccatccgct gctgtgccgc
    27721 gaatttctac cgggaccctc attacgactc gagcgccttg ccggggatta tcaaattgtg
    27781 gtgtatcaat caagctaatt tgccgtagcc actccaaaca aaatgtccca tagattatga
    27841 cgtaccacct tgcttgtcaa ccctgaacta actgaaaagt aaagtatttg tgaaaataaa
    27901 ggctgtgcga ttttttgaag tctacagttg tttcaaggtg aaacactcgc gtggcctcac
    27961 gctgcgttga caaatgctat cgtcggcggg cacaatcaat ttgcctcgta atacttaccg
    28021 acgtgcccga atggcggaat tggcagacgc gctagcttca ggagctagtt ctcgcaaggg
    28081 agtggaggtt cgagtcctct ttcgggcacc atgtcttaat aacgaaaatg tgcggaagtg
    28141 agggtgcgcc gtggaccggg actacgagat atttgaatgc ctatcggacg gctttgtgcg
    28201 gtggcgcgcc cgcgagagtg ggctgcgcaa tgctagatta aaattggacg gattgtcgcg
    28261 ggaaacgggc aacgaatact tcgcgatgca tctcatgacg cgggatataa tcttcgccac
    28321 agacgaatcc agggccgaat ccggcaaagc cacaaagcga atttttcaaa tcgcctacac
    28381 cgaagcgcta cgcatttcca ggtctgaaat attaaggagc cttggctatc cggtggtctc
    28441 agtaatcggc aacgaggcgg caatgactct cctgactacc cttcgccgcg acaatcttga
    28501 tatcgctgtt tttattgttg gccatgcggc cccggcggat accagaaaag aaatggtcga
    28561 ttggctcaag gcgaattatc cgacagctaa agttctcgcg ctaaattcgc cagaccagca
    28621 aatagaactc gcggattaca acgtgaagca aaacggcccc gaatcgtggc tgcctctggt
    28681 gacgttaaac atttcccctt tgcaggccag ttaaacgact ccagcttaac cagccgccgc
    28741 attagctttt gaccggtgga cactcgctgg cttaggtggc agaaaatcgg gctgtatatc
    28801 cagcgtaaaa aattcatttt ccgagtgttc ccttagctcc tgcatctttc tttgtgcatc
    28861 gtactttccg gatacgcacg ttcgccagag cgtggaacca tcgggaaatt tctcgaatat
    28921 ctcgtactca cgccgcatag gggacacctc attcgacgtt tttatccgga tgccttgcct
    28981 cttaccgctt gagagataac cgtgccgtta tatacagatt cacagaaaat caacaataca
    29041 gcgattgctg tagcggaatt ttaaaacatc agcacatttg cgagctgtgc gaatcggctc
    29101 aactgggcgg aaattgtggt tgcattcctg attctgttaa cgtcccgggt ctaaatctct
    29161 catgataacg tgaagaacct gcatggagcg gcgggcatcc tgctcggtaa cctgcaagtt
    29221 tgcattgccg ttcgcatcca tcgaatcccg atatccgcgc gcaatttccg gcagtcgtgc
    29281 gcaacctccg cgaatcagtc ccgccctttc cgcgacaatc agtcgttcgt gaaaagtcca
    29341 atcggaaatt ttcccgttga ggtcatccgc acaggccatg gcatcgacgc cattgtgttc
    29401 aagcgcatcg gtcacgatag cttcgaggat tccgcatgaa ttggtcagcg cttcttcgta
    29461 ttcgcgccgc tcgagcgcat cccggctatc cgcgtatgcc tgctcgagaa cgggacggat
    29521 ttccacattg tgaacaaatt caaagcgccg gggctgcggc gtggcggagg cggaagcggc
    29581 ggaattttct tcgctgccca tgcgcgagag cgagaggacg gcctggtcca gaacatggac
    29641 gagccgctca acaatgactg caggcggcac cagatcgatc gcttcacctg aatctgtccg
    29701 cagcaaaaac gcttcgctga atgcacgagc aagccaatgg gccttgcttc cgcccgataa
    29761 ttggttcatt gccacgccgc aatcctgctg ccaggcacga ttggctacga tcaaggattt
    29821 gttggtgggg tccgcggaca aggattcacg aagcttgtta ccgcgacgga ttaaatgatc
    29881 gaggtgagaa agattcgagt cgatctgcgc gcgcaggctt tcttccattt cgtgcgcctc
    29941 cgcctactta gtgggggaat cagaaccctt cttcgcgtgc agttgtatat cggcaatctg
    30001 gtcgatgtga ttatacatcg cctgcgatgc gccttctgca tctcggcgtc gcagcgcggc
    30061 aaccacagct tcgtgcccgt cgatcgatcg tgctgggcgc cccttttgca gcaaggaact
    30121 cgtgcgtgac tctacaagca gatcgttgag ggtcgccatg atgctcatga ccacgcggtt
    30181 gcgcgtggcc cgcgccagaa tggcatgaaa cgctgtgtct tctgaaatgg cggattgacc
    30241 tgttttcagc tttagccgct gcgcatccag aattttttgc agctctgcca aatcttccgc
    30301 ggaagcgcgc aacgccgcca cacgcgccac tgcaggctca aacattcgcc ggacgtcgag
    30361 cagttcgtcc tgcaaatccg cgcggtaact cattacggaa gcaagaggag caaccagccg
    30421 atccactgaa agctcgagcg gaaaagtacc ttgcccatgg agcgtttcca cgaggccaat
    30481 ggtttccagc gtgcgcagtg catcgcgaat cgacccgcgg ctcaccccaa atcgctcggc
    30541 aagatgtcgc tcggagggca gaggttcccc tggaggaaag gtgccgtcca gcattaacac
    30601 acgtatcctg tcagccactt cttctgcgat gcgcgtcttc ttgattggag taataacgcg
    30661 ggaatcttcg gaggagggct gggccaaacg cttgacagtg ctgcgctccg gtgttaatct
    30721 ttcgttgtca actggttcaa tcactgatcc agtgtaccac agaatcacat gagcgaaaag
    30781 ctttttgagt tcatgactgc tggctcgccg cgcgccgtct ccggtgccat tgaacaattc
    30841 gctatcggtc taggcagcct caccgccgta gttgtccctt gggaaagcga taaaaactct
    30901 ttgtgtatgg ctgtggcctc cactcaaggc gaaggctggg ccctggagca tacaaacatt
    30961 ggaacgctcc gccttgtgga cctcggaaat gaaaacacgc ggatcgaaat cttttcggag
    31021 ttgaaggatc acgcggagca acagaagtta gccaaccttt tcgctcgctt cagcagtcaa
    31081 cttcaatccc aattccaggc ggctcagtga cggaactcac cccctccacg ccggccacga
    31141 cgctcacttt gcgcgtggcg gaagcgcgtg tggaggacgt gggacgcgcc atagcgcgcc
    31201 ttgctccttc tgatctgcag cgtattggcg cacagtccgg cgacgtggtg aaagtgacgg
    31261 gcggcacctc gtctgtcgcg cgcgcggaaa tctccgacga gaagcatgaa ggattcgtgc
    31321 agattgacgg cacggcgcgc agcaattgcg gtgtcggctt gcaggagcaa gtcaacgtaa
    31381 cccccgtcga gcactcgcaa gctctggccg tgcggctctc tcccatgtgg atcggcgcgg
    31441 ctcccgcaat cattgccgtt gaacgcattg tcgaagactt gacgggagtg ccggtcacgc
    31501 agggctgcat cgtgcgcgtg ccgacctttg ccaaggccgt aaacttccag gtagtgcgca
    31561 cgattccttc gggccccgtg atcatcggcg caaaaacaga tatccgtatt gtggaaggcg
    31621 agcagaccgc ggttcgcgcg cccgctgtct cgtatgaaga tattggcggt ctcgaaaggg
    31681 aagtcgcgcg cgttcgcgaa atggtcgaac tgcctttaaa acactcgcgc atcttcgagc
    31741 gattgggaat tttcgcaccc aagggtgtgc tgttgtacgg acctccagga acgggaaaaa
    31801 cattgctggc gcgcgccgtg gctgccgaaa gccgcgtgca ttttatttcg ctgaacggtc
    31861 cggaaatcat gcgcaagttt tatggcgaaa gcgaggccaa gctgcgcgaa gtttttgagg
    31921 aagccgcgcg ccgcgctccc gcgattttat tcatcgatga aattgacgcc gtcgccccga
    31981 agcgtgccga agtttcgggt gaagtcgaaa agcgcgtggt cgcgcaactc cttggcttga
    32041 tggatggctt cgtatcgcgc ggccaggtga ttgtgattgg cgcgaccaat atccccgaag
    32101 tgctcgatcc ggcgttgcgg cggcccggac ggttcgaccg cgaaattgaa atcggtgtgc
    32161 ccaacacgca agcgcggctg caaattttga agattcatac gcgcgctatg ccgcttgctc
    32221 ctgacgtcaa tctccaggaa attgccgaac attcccacgg attcgtggga gccgatttgg
    32281 aggcgctttg tcaggaagtc ggcatgatcg cattgcgtgg ctttcttgcc accgcgccgc
    32341 tcgaaacaga tggcgctcct cctgttgacc tgagcggatt gcacgtgacg cgcgaggatt
    32401 ttctagctgg tttgaaagaa gtcgagccca gcgcgacacg cgagttcttt atcgagaaaa
    32461 gcacaacgac attcgccgat cttggcggca acaccgattt gaagcgcttg ctcgattccg
    32521 tcgtcgagca ctcgcacatg cacaacgaaa tttacgatca ggtgggcctc gcgccgccgc
    32581 gcggcattct gtttgtcgga ccgtccggca cgggaaaaac ggcgatggca cgcgcccttt
    32641 cgggcgagaa gcagattccg cttatcgcca ttgatggccc gcaactctat tccaagtggc
    32701 tcggcgaatc cgagcgcgca ttgcgcgaag ttttcaaaaa agcgaggcgc gctgctcctt
    32761 gcatcctttt ctttgacacg attgacgctc tcgccccgaa gtttggcgtc gaccatcttg
    32821 gctccgacat ttatcaaaga attctgagcc agcttttgcg tgagattgac aacttgcgtg
    32881 acgtaaaggg cgtcctatta gtggcggcaa cgaaccgacc tgagcgcgtt gaacccgcgc
    32941 tcttgcgcag cggcagattt gactatatcc ttccgttcgc gaaacccggc gccgcggacc
    33001 gggccgaaat catgcggctc tgttgccgtc gagtgccgct tgcgcctgac acggatgttg
    33061 atgggttcgc atcccagttg gaaggctcga ctggcgctga tattgaaagc ttgtgcaaga
    33121 aagctacgtt gctggcgatt gcaaaatttc aggacggcgc gcaagacgcc gctttcaccg
    33181 ttcgacgaag tgactttcaa tccgttctgg attcccatcg tggtagccca gctcagatga
    33241 aagatttggc cgcgcggtct ggaaacgcgg agggatttac tttggcacgt atccatctcg
    33301 acgacaaaga ggttcgatca tgagctacac agaactcaca gtttggacgc gcggcatcat
    33361 catggacaaa gagggccgcg acatcgttaa ttccgtcggt gcttcagccc gcaccgaagg
    33421 caagttttcg cagtccatgg aaaactatgt cgataacccg gaccgcacca acgcccctac
    33481 gcgcaagtat tgccgcgtga gcgacagcgt catcgaaaac gcgctgacct atgagaacga
    33541 gcatccgaac atcgtggtcc tggtcgaggg caccatggtc aagggctggg actatatgcg
    33601 cggaatgccc cctggcggca cgctaatcat caacacgcac tacacgccag agtacatgat
    33661 ccgcttcgtg ccgggagccg aacgcctcgc gcagcttgtt tgcgtcgacg ccgcaaaact
    33721 ggctgaccac aagtggctct actatcgcct cggcgaactc ggtctcgacc gtctctcgac
    33781 ggaaggcgct gcggaacgca gcaaattagt cgctcccgat attgctgctc ccctgattgc
    33841 ggctgttgtg aagacaactg gaatcgtaaa gatggaaaca atcgaaccca tgattgcgaa
    33901 taaagcggcg tttcatgcgg ctcttaaaga gctgcacatc atgaagttga acccggttac
    33961 tgcttagaaa aaaatttgag gctgcgggct ctacttgttc accctgattc cagaagggga
    34021 acccgccaga atgagggtca aaaatgtcga ccaaaggtac acacattccc gattggctga
    34081 acgtgccgtt tgccggaatc actccggcgc cgacacagga aaagggccag gatcttttcc
    34141 cgaccggaaa ctggcgcgca atccgcccgg tttaccgcga taagctgccg ccctgcaaca
    34201 atgcctgcgg cacgaatgaa aaaattcagg gctacttgga tctcgtgaag cgcggcaaat
    34261 atctggatgc ctacgcgctg atcaaggaag acatgccgtt cccgtccgtc accggccgtg
    34321 tgtgctacca cccgtgcgaa caggcctgta accgcggaca gtacgacgaa gccatctcga
    34381 tccgcgcggt ggagcgcttc cttggcgacc tcggccaggc gttggcaaaa gacgtggtga
    34441 aggctggccc atccaacggc aagaaagtcg ccgtcattgg ctcaggtccc gcaggccaca
    34501 gcgctgcgta tcaactggcg cgcatgggct acaaggtgac aattctcgaa aggtcgccga
    34561 aggcaggcgg actgaatcgc ggcggcatcc ctgactgggt tttgcctcag gcagtgctcg
    34621 atcgtgaaat tcagcgcctt gtcgaactcg gtatcgagat caagacgaat gttgaagtcg
    34681 gcaaagacat tacgcttgac gacctgaaaa agaattatga cgccaccgta tttgccattg
    34741 gcctcacgga gtccaacagc gcacgagccg aaggagaaaa caaagctggc gtgatctatg
    34801 gcctgccgtt cctgcgcgac attggcatgg ggacatccaa agtaaaactt gggccccgtg
    34861 ttgctgtgat cgggggcgga aacaccgcca tcgattgcgc gcgcgaagca ctgcgccagg
    34921 gcgccatcga agtgtccatg attacggtgg aaggcattaa ggatgaaatg cctgcctcgc
    34981 cggaagatct gcacgacatg cttgaagaag gcgtcgagtt gatgcacggc ctcgccatga
    35041 cctccgttct cggtgatggc aaagtcgaag ccctgcaact tcatcccgcg cgtttcaccg
    35101 gcaagatcaa tgcttcgccc atcgccatcg atcgcgaagc taccgccaag aaattcgctg
    35161 tcgacaacgt gatcattgct gttggacaaa gagcgaagct cgattggatg cccgcggaat
    35221 acaaaaccga acgcggcacg atcaagattg acgagttcgg ccgcatcgcc aacaccaact
    35281 ttttcgcagc gggcgatgtg gtgcagctcg gttccggcca gccgctgatg gtcgtgaacg
    35341 ccgtcggtga cggcaagcgc gttgcgttca atctggacaa ggtcttgcgc aatgctcctc
    35401 tggagccgcg cacggttccc atcgatgtaa tcgtcgaact ggcccgcatg aacatgacgt
    35461 atttcccgca ctttccgcgc gtccagcaag ccatgcttgc tcccgcagcg cgcaaaagca
    35521 ctcagcagga agtgatccag gcgtactcgg aagaagaggc cacagccgaa gcagcgcgtt
    35581 gctttagctg cggcacctgc aacgcttgcg acaactgtta cctcgtttgc cctgagcctt
    35641 gcatcgtgcg gtccgtgcgt tcgaacggcc tttacaaaat tctggtggac tactgcaagg
    35701 gctgccgcgt atgtatcgaa gagtgcccaa ccgggtgcct cgaaggggtg cccgagctgg
    35761 atttcgatac aggcgtagtt cgaatggata cggccttcgc gatttcgcaa ggattgcatg
    35821 gacgtcaggc ggaagagttg gcgcatctgt cagaccgtcc ggcaaagaat gtctaggttt
    35881 tgtagcgaca ggcttcagcc tgtcagggtt tgaattttgc gttgttaatt gttagagaag
    35941 tcaaaagcgg accggctaaa gcctgtccct acgaaagtca aagtctttca gcgtccgccc
    36001 ataggtggaa cgcgcggagt aaattatggc aacgctcgta gcaggcaaaa agaagacagt
    36061 tcgaattaac ggctgtgtcg cctcggctca ggcagccaag tacgccgacg tcgacgtcat
    36121 caccgcgtat cccatccgcc cttacacggc gaccatgatg gcgctggcgc aaatggtggc
    36181 caacggggaa ctggacgccg agtacatcgt ggcggacagc gagcactccc agttgagcat
    36241 cgcgcacggc gcttcttcca gcggtgcgcg cgtcttcacc ggcagctccg gcgttggcgt
    36301 gcagtacggc tacgagctgt actcgccgat ttccggcagc cgcatgcctg tgcagatgat
    36361 gatcgccgac cggacactcg atcccccggg cgacttcggc tccgaacaca cggacgcgct
    36421 ctcgacgcgc gacatgggct ggctcatggg ctggtcatgc gatgcgcagg aagcttttga
    36481 caaccacttg atcggttacc ggattggcga agatccgcgc gtgcttttgc cacagatggt
    36541 ttgccaggac ggcttcttcg tttcgcacat tgtctgtgac gtagacttgc ccgatcaggg
    36601 ccaggtagac gaattcctgc cgccttacaa gcatccgtat ccgctcgatc cgcgccaccc
    36661 tgtgtcacac ggtcctcaga tcatgccgga gcaggggccg cctttgcagt tggaacgcgc
    36721 gcgcgccatg gaaggctcga tggcagtgat cgaacaggct cacgaagaat tcgcaaaaat
    36781 cttcggccgc aaatatgatc cgtggatcga agaatacatg acggaagatg cggacgttgt
    36841 cttcttcttg cagggcgcgc acgccgtcac ggcgcgctac gccatcaagc acatgcgcga
    36901 tcgcggcgca aaagttggct tggtgcggct gcggacaatc cggccgtatc cgaccgagca
    36961 ggttaaggaa tcgctcagca agttcaaagt ggtcggcgtg attgaaacaa acatgggctt
    37021 gggcagtgtg agcagcggtg gttcgctcta cgcggaagcc gccgcggctc tctacgaaag
    37081 ccagaagcga cccttgattg tctccttcat ggctggtatg ggtggcgaag cgattgtgct
    37141 taaggaattt tattggatga cgcagaagat gctggaagcg caaaagcgcg gcagcatcga
    37201 gaagcgcacg cattgggtcg ggtttgagga gtaattgatt ttgagtgtag ccattttatt
    37261 gatgcgacgg ccttgaagtt tttgcactaa aatctattgg gtgcgaattt tcaggaccgc
    37321 catccggagg aaaagatatg tccaccattg aaattgagcg acagatactg cctatgttcg
    37381 gcagcccgaa tcagggggcg cggttttaca atcctgaatt gccgtccagc gaaccattcg
    37441 tgcccggcca ccgcacatgc gcgggatgcg ggccgtccat ccagtaccgg atgacgagca
    37501 aggcttcggg cgacaacacg attctcgtcg ggccaacggg atgcatgtat gtagcgaaca
    37561 gctcgtacct gtgcactccg tacaatgtgc cgtgggcaca cacccagatc ggcagctccg
    37621 gcagcttcac cgcaggggtc tcagccgcct atgaagcgat gattcgcaaa ggcaaataca
    37681 aagggaagtt cccgaatgtc atctgcgaag ccggcgatgg cagctcgtca gacatcggaa
    37741 tcggctcgat ctcaggcgcg ctctatcgca accacgattg cttgatcatt tgctatgaca
    37801 acgagcagta tgccaacacc ggtattcaag cgtcatcgcg cacgccttat ggcggcatga
    37861 cgacgttctc gcctccaggc ccgaaagtgc cggaagcgaa gacgctgttc cctaaggatc
    37921 tcgcgcgcat gatggccgca ggacatccgc actgctacgt ggcaaccgcg agcgtcggat
    37981 atccgatcga cttgatgaac aaagttcgca agggcctcaa cttcaagggc gcggcatttt
    38041 tgatggttta tacgccttgc cagaagggct ttgtgtatga gacgccgcgc tccatcgatc
    38101 tcggccgtct cgtcgtcgag tgcgggctct atccgatctg ggaatgggac aacgacaagc
    38161 gtgcctatga ctacagtttc aagccgcaat ccatgcgtcc ggtttccgaa tacctgaagc
    38221 tgcagggccg cttcggacac ttgcacgccg agcacatcgc cacgctgcaa aagttcgcca
    38281 accagcaatg gcagatgatg ggcgttcaac ttccggaagc cttgatcaag gcgacggacg
    38341 tgaaaaatct ttcgaacatg ggctctgccg aagcggcagc gtccctcgcg gagaccgtat
    38401 gagcaccaat ccatcatcca acgcaccggc agcgcagcag gaagcgtata tcgtcagtga
    38461 aggaaagaag cgcggctcga ctcgtgaagt acgagacgaa gccgcgtttg accgctacta
    38521 cactgtggta aaagtgcatg ctggcgacgc aaacctcacc gacaactggc acacgaacga
    38581 agtgctgagc cacgcaaaaa ataatttcga aggatacacc ttcgtcatgc gcacgctcga
    38641 agcttggggc gccatgatcc agaacgatca ccgtgcgtat tacaccgccg gcggccgcgt
    38701 ctgggttttg gatctggaac cggccacggg aaagaagccg agactgcccg gcaattacgg
    38761 aacgaagtaa ccgcccgttg ccgcgcattc tgctcattga cgatacgccg gagatcgccc
    38821 agcttttgtc gttttctctt cgcgaccacg gctttgaagt ttttgcctcc gggtttaccg
    38881 actctatcaa tgagttggtt gcggaacaga aagccgatgc gctcgttttg gattgttccg
    38941 tgtacgacat gagcgaaacg cttttcgata ccgtgcgctt cgacgcgact aacgctcagc
    39001 ttccagttgt catcatcagt gacacaccgg aagaagcgga taaaagtctt cgctcccgcg
    39061 aagcgacaaa tgttatgctg atcccaaaac catttaccgg gtcacacgtc gccagggcgc
    39121 tcactgaact gcttaaatcg cgcgaagcac gtgggcaggg ataaatatgg cttcctggaa
    39181 catggataac tggcgctacg tcgagcgcca gtccgtaacc gaccccaaag gcacgcagtg
    39241 gtccgtggcg ctgatggaca ttctggggca ggaaggcgat cccgagatgc ccaacaagtt
    39301 gcaggaactt caatattctt caggcagatt ttttactttg gtttattccg ccagcggaag
    39361 cgtagtgtgg gaacgcggtt acggcacatt gcccgaagcg acgaaagcct tcgagcgcct
    39421 catcgtcgac gtcatggacg gcgtagtgga ccccacgcag cctacgttcc gcgaagattt
    39481 ggaagattaa cgaaggatca ggcggcttca ttctgacttg tccgatgtaa ttgttcccgc
    39541 cgaaatttta ctgagcctta aacatttccg gcaacccatc gacggctcgg cccacggcac
    39601 gcgccagatt cagcttggca atgttgtagg cgaagacgcc attgatataa tcgaactcgg
    39661 cgatcgttac ttgctcctgg gcttgcacga actgaacgtt atcgatcacc cctgcttcca
    39721 ctttttggcg cgtcaagtcg agcgcctctt tcgtgacctg tacatttcgc tgcgagactt
    39781 ccacttggct tgccgctgcc tccaaatcca gataggcgct gcgcacatcg ctttcaatct
    39841 ggccttttaa atcttcatac tcggcgcggc gttgcgcaag tgctgcatcg gcttgctgaa
    39901 tatcgccctc ggtgcggcct ccctgccaaa tgggaatctt caggctggca gtcgctgtgt
    39961 acgtggtttg caattggctg gggttgacgc cgatgccgcc gtaatcgcca gtcactccga
    40021 gcgaaggata acgctcggcg cgcgcggccg cgtgcgaacg ttcggccgcg tggacctgtg
    40081 cgtcggctgc ttttaaatcg gcgcgctgct gaaacgcctg cgccagcgcg gcttccatcg
    40141 tcaaaggttc cgtgggtgcg aagggcacat catccgtgag ttcgtattga tcgttcggcg
    40201 gtaggccgat catgcgcgcg agatttattt tttgcttggc gagatcgttt ttgagcgaaa
    40261 gcaggcgctg ctggtgcgtc agggcctgca cttcattctt atccgcgtcc actttcgcga
    40321 tcaggccctg gccgagttgc tgtttgctct gatcgaacac cgcgttggcg ctatcgagtt
    40381 gcgattgttc tgaatccatt cgcgcttttg cggcaatcgt ttgcagatat gcgccaccca
    40441 cggcgagcac gatcagttcg cgggcatctt gcgccgaata gcgattcgct tttgcgacat
    40501 cgtttgctgc gcggaaatta ttaatggccg cgagatcgaa aacgttctgc gataggtgtg
    40561 cgcgcaaatc catgtaatta aaagggccga tcaccgcggg aatcgcgaag ccgggaaaat
    40621 taaatcgaaa accctccgcg cgcaaatcgg ttgtttgaac attttcggtg actgtgccat
    40681 tgatattcgg cagtaaactg cttagagcca ctttgcgctg tccttcggct tgcctcatcg
    40741 tttgcgtcgc gcccgattcg ccgaggttaa aagatagccc gcgctgaatt gcatccttaa
    40801 agccaagttt tccgttgaac ggcattttcg ccgtgcccgg cgtgcttccc gcgtaattac
    40861 cttgcacgtt cacacttgga ctgagcgtgt tcacggtcgc cgtagtgctt ggcgctggcg
    40921 cttccgaagt ttttactgtg ccgccggatt gcgccgtgcg cccggaaagc ggtagctgca
    40981 cggcctgcgt gccttgcgat gatccggatg gaggcccaag ttgcgcgaat gcgggcgtgg
    41041 caaataatag cgcgaccgca acgaacggcc attgtttgcc aaatcgtcta gcgctacgat
    41101 ttgagaagaa ttttccagga attcggaaat cgattttctt gcgcataaac gctcccggct
    41161 tctttctcac gacaaagtaa atcgatcggc tctcgtggcg cttgcatctt gccggcggat
    41221 gttcgctgac ttcgttgccg cagaaaaccg ccggcgagac gccggcgcca caaaaccact
    41281 caccctaact cagctgcttc ctgaatcgcc aaatactcag cgcaaccagc gcgatcgtaa
    41341 atgccagcag cgccaggaaa ttcggccaca gcgtatccat cccgcttcct ttcaacattg
    41401 ccccgcgaga aatcattccg aaatgctgaa tgggattaaa ttgcgcgaga ggctgcagcc
    41461 atttcggcat ggcttccacc ggagtaaaag cgccggaaag agaagtcagc ggtggattta
    41521 caaaaaataa tgtgagctgc gcctgccgcg cggatttcgt gaacgtcgcg ataaaagtgc
    41581 caatgctgat tccgcacaat aggcaaaggg cagcggcggc aatcaccagc aaaacatttc
    41641 cgcggaaggg aatgccaaaa acaatgcgca tgagcgccac cgccaacacg cccatcatgc
    41701 aaagcagcac aaagagcgga aaaagtttgg cgacaataat ctgcgtcgtc gaagcgggag
    41761 acattaataa ttgctccagc gtacccgctt cgcgttcctt cacgatggcc gctgcggaaa
    41821 tcaacgagcc gttcaatatc aaaaggacgc ctaaaattcc ggtcacgata aaccaggtgg
    41881 gttcgaggcc ggggttgtaa aggtaggcgg gatggagcag caccattccc agatggctga
    41941 catcgggcgc ggcaatttgc tgaaattttg tgtggattcc ttcgcccgcg agcgtctggt
    42001 tgtaggacgc gatcacgccc tgcgcgtacc cctggccaat cgtggcagta ttggagttca
    42061 tcgcgttcag caggatttgc accgtagcgg gttggccccg ttgcaaatcg cgtgcgtaat
    42121 cgtagggaat taccagcccg gcatcgaggt ttccgttcgc aatctttttg ctcaattcat
    42181 tgatgtcgaa ataagttccc gcgcccttga aactcttgct ttccgtcatt gtcgcgacaa
    42241 gttcccggct ctcgggcgtt ttgctgtcgt ccactactcc catgcgcaga ttgctgacgg
    42301 ttgcgttcag cgcaaatcca aagatgagca attgcagcgt aggaggaagg atcaaggata
    42361 tggccaggcg cttgtcccgc aaaatctgcc ggaattcctt gcggatcaat gcccgcatgc
    42421 gatagccgag gaaagctcga attttattca tggtttaatc cttcaactgc atgtgccgca
    42481 ggttgcgcca cgcgatcgtg tagaaaatgg cgccgatcgc ggcgatcgcc agaactttcg
    42541 accacaccga gggccagcct ccgcccgcga gcaacgagtc ccggacaatc tcaatgtagt
    42601 agcggcccca aacaatattg gaaatccacc gaatccccgc cggaatattt tcaacaggga
    42661 agagtaagcc ggagagcata tacacaagca aaaatcccgt gagcgcaacg gcttgcatgg
    42721 ccgccacttg gctggggatc gccgcgccga tcatgtttcc gaatgtcgtc accacaaaca
    42781 tgtacaaaat agtggagacc agaaacggcg tcggatcccc ggcaaaacgc agcccgaagc
    42841 ccatgaataa aagcatcatc agcacgatgg cttccgccgt tgccacgagc atgaatgcgg
    42901 caatcttgcc aagcaggaat tcatgcgcgg aaatattcga aacgtaaact tgcaggatcg
    42961 ttttttgctc accttccttg gacatggcca gcgtagccag cagcggggga aaaagcgaca
    43021 tggccagcac ataaatcccg ggaccataaa atttctgtga ggagcggctg gggttgtacc
    43081 acagacgaat cgcggcgttc accggctgaa cctgcctcgc gccagcgttt tccgcgttgt
    43141 aggctcgaac aatttgcgcg gcatccccgg aaataatttg ggctgtgttt tcgtcggacg
    43201 catccacgag aatctgcacg gttgcgtttt cgcctcgcgc aatctcacgc ccatattcct
    43261 cgggaataat taatgcgcct cgagcgctgt tggaagcgaa cgcttcttcc ggctctcgat
    43321 ccgcgggcca cgacgtaacg tggaaactga gcgactgccg aaacgtatcc accagttttt
    43381 gcgacgcctg cgattgatca aggtcctgca caatgatggg aagatctttc acggtcagcg
    43441 aaatggcggt tgtctgcagc aacagcaaaa ccaccggaag gaacagtgcc agcgctaccg
    43501 cgaggcggtc acgtagaagt tgtattaatt cttttcttgc ctgcgcgata attcgccgca
    43561 tgattcgtcc ttttattctt cagagccaat cttgccgtgt tcgcgagcct tctccacaac
    43621 gctgataaag acatcttcca gtgagaagcg gccttcgcgc gcatcgatca cgcgaattcc
    43681 tcctgctgac aggcgctgcg tatattcgcg aatgccggtc tccacgtcat tgtccgtaat
    43741 gatgtgaagc cggttgccga aaagcgccac gcgccagtgt tgcgtttcgg ttttcaataa
    43801 atcggccgcg cgctgtggct ggtccacgcg gaattccagc agatgacctt tttgctgtgc
    43861 cttgatctcg cttggtgagc cttccacaag cagctcgccg gccaccataa aacccaggcg
    43921 attgcactgt tccgcttctt ccagatagtg cgttgttacc aggaccgcag ttcccgcgtc
    43981 ggccagatga ttgatcatca tccagaatgc gcgccgcgcc agaggatcga cgcccgaggt
    44041 tggctcgtcc agaaaaacaa ttcccggttc atgcataatg gcgctgccga acgcaacacg
    44101 ctgtttccat ccgccaggta agcttcccgt gatgagctgt tccttgccct gtaggccgga
    44161 aaaatcgagc acccagcgct ctttctcggt taattcttcc gaggggacgc catacacgcc
    44221 gccgaaaaaa tcgagattct ctccgatgga aagatcgtcg tagagcgaaa acctttgcga
    44281 catgtagcca atctgctggc gaatgtcagt cgagcgaaat gcgccgcgct cgcccgcaag
    44341 ctgcatattt ccggtcgaag cttccagcaa accgcatagc attttgattg tggttgtctt
    44401 tccggcgcca ttggctccca gcagcccgta gatttcgccg tagcgtacct gcagactcac
    44461 gtcgtgcacg gcagtaaagg acccgaactg tttcgtaaga tgttccgctc caatcgcaac
    44521 ctggccgcgc tttgccttgt gatcgtggcg cgcggggaag ggcgtatcat gcatttctcc
    44581 accgagggaa cggagagtcg cgacaaaagt attttcgagc gttggctcat ccattctgac
    44641 gctatcgatt gcgattcctt ctctacgcat tgtttcttcg attttttgtc tggcggcatc
    44701 gggcgaatgc gagagcaagt ccaggcgatc tccaaaacgc tgcacatcaa aaatagcccc
    44761 ttcgcgcccg gaaactttcc ccatggcttg cagggctttg cgcagatccg gcgaacgcag
    44821 ctcgatgcgc ttcgcatgca agctctcgcg aaaatcatcc ggtgtgccga cttgctgaat
    44881 ttcgccgaga tggatcaacg cgatgcggtt gcagcgttcc gcttcgtcca gatacggagt
    44941 tgccaccagt acggtgagcc cctgtccagc aagatgcgca agcgtgtccc aaaattcccg
    45001 ccgcgaaacg ggatcgactc ctgtcgtcgg ttcgtccaga agcaaaacat ccggctcgga
    45061 aacgagagcg cacaccagcg ccaacttttg cttcatgcca ccgcttaatt gcgccgcaag
    45121 cctgcccgag aagcggtcca tatcgaacat ttttagatag cggccgccgc gctctccgat
    45181 ttcttcgggt ggcacatggc gcaaatcgcc gatatagcgg atgttctcca aaacagtgag
    45241 atctgggtac aggctgaacg tttgtgtgag gtaacctgtt cgcgaacgtg cttcccgtgc
    45301 cggtttgcca aaaatttccg cggaaccgcc gcttgcttcc atcacgccgg ctagaatttg
    45361 aaatgtagtc gtcttgcccg caccgtccgc gccaatcaat ccaaaaatct ctccgcgctt
    45421 cacatcaaaa gaaattccgc gaaccgcttc gagcgtgccg taattttttt tcagattgcg
    45481 gatatcgatg gcgacatcgg aagctctcgc tgatggcgca ggaggatttt cgcgatctga
    45541 agcggcggta ttcattgcgc tgctcgtttg tgaggtggcc aggcattgcc gttggtaagg
    45601 atttccccgt ccgcaggcat gccaggtttt gcaaatccga ttccttgcgt caattgcact
    45661 ttcacgccca ccacttgctt cacgcgatca tcgcgaaaat atgtattttc aggagtgaag
    45721 gtcgcttgtg gatcgatccg caaaacgtag ccatcgagag attgattcgg attggagtca
    45781 agaaaaacgt gtgccggctg gcccactttt attttgccga tattgccttc cggaacaaat
    45841 ccacgcagat aaaccttgga aagatcgagc aaagtcacga tcgccgtgcc agcctgcacg
    45901 acttcgccgg gttcggcggc gcgagtgagc accgttccgg aaaatggagc gaccactgtt
    45961 aaatcggcgc ggtcagcctg cgcttgcgcc agtgttgcct gtgcttgcgc cgtcgacgcc
    46021 ttggcagagg caatcgtgga ttgctgctgc gcgatttgtg cctccgtacc ggacacttgc
    46081 gctctgcgaa tgcccggtgt gtccagattg gcctgcgcgg tggtcagcgc tccgcgagct
    46141 ccctccactt tgcgctgtgt agaagcgacc acggctgcct gctgatccgc ctgcacctcg
    46201 gcctgcaggc cttgcaattg cgatgcggcg cctgttttgc tcagtcgcgc gtacgcgtcg
    46261 cgattaaatt ccgcgaggcg caacgccgct tgctgctgca cgagatccgc ttccgcggcc
    46321 gttaagtccg cttgcgcttg ccgcacgcgg ccctgcgcgt ccattgttga ttgtccagtt
    46381 tgcaattgat tttccgtcag ctgctcttgc agtatggcga tctggtcgcg cgcgctctgc
    46441 agcttggctt gcgaatcgag gagcgcagct ttcgcctgat cctctttagc gcgtacttgc
    46501 gcgtcatcga gccgcgcaat cacttgccca gccgtaaccg tgtcgccctc gcgaactgtg
    46561 atttcgagaa tttttccgtt tgttttcgga gctactgccg agtcgtcgcc ttcaatgcgc
    46621 ccgctaagcg cgacgatgct ggcaggaaga ttcggcgtgg cgaaaaataa ctcccaaatc
    46681 aaaacagcgg cgacgatcac gccgagaatt attaaaattg tgctaacgcg tgagcgtggc
    46741 tctttgttta cttccggtgg cgcacttgct tgatgaggtg gcggcggtgt gcgctgttcg
    46801 gtactcggcg gcgcttgcgt catcgtattc gtattatttt ttgtttcatc catggcgttt
    46861 cccatgcccc actttcttca ggtaggcgag cgaaaaatcc gcaatgtggt cagcaatgcg
    46921 gtccaattgg ttgggagtga gtttcaattc cggttgcaaa tgcgctagca cagggcgcga
    46981 aaatgtgtaa aacaaaatct gtcccacgat gctgttcacg gagaggcgcg tcgtttcatg
    47041 atccggaggt aacccgatca cttgccccac cgctttcttc atgcggtcat agatcggccg
    47101 catgccttca tccaccacgc gctgcatcgc gccggttggc tgcgagcatt catgcatcac
    47161 gaggcgaatg tgccagtccg gcctctcttc ctggcacatg ctcatcaacc gcgcgcgaat
    47221 cacgccgcga attgtgtctt ccggcgaaag tgcggtatcg agcgccgcgt tcatcgccgc
    47281 cgttttggcg gcgcgcacgg aatgtcgcag cacttccgtg tacaatccca tcttgtcgcc
    47341 aaacgtgtaa ttcaccgcgg ccacgttggc tcccgcgcgg cggcagattt cccgaatggt
    47401 cgtcgcgcgg tatccgcgct cggcaaaaac gtgcccggcg gcatcgataa gtttgtccct
    47461 cgtaagctcg ggtgctttgt gcatagatcg tttgcgaacc acggaagatc ttcgtgaagg
    47521 agaagtgtgc agcataacaa acacacgttt aaaacaactg tttgaatttg tcaagcggaa
    47581 aaaactgtga ccggcgggcc gcatctgctc cgcgggggcc gccatcttaa ttttcaaaaa
    47641 ttaaagcaat cccccgctag ccgcattcgc ccccagatgc taaaatcggg aaaggcgttt
    47701 ttgttcgtta ggcccgtagc tcagttggtt agagcgctac cttgacac
//
LOCUS       DASZMB010000036         3522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4161081, whole genome shotgun sequence.
ACCESSION   DASZMB010000036 DASZMB010000000
VERSION     DASZMB010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4161081"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /gene="hisS"
                     /locus_tag="VG272_01660"
     CDS             complement(<1..830)
                     /gene="hisS"
                     /locus_tag="VG272_01660"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HWF12415.1"
                     /translation="MNRFGSMNLENPSNSQAEEGFAQCLTGLKTALRSFRFGDTLALR
                     PEATASVVRAYIEHGMHVHPGNVKLYYMGPMFRRERPQKGRYRQFYQIGAEVLGSSDA
                     PGIDAEVIEMLMRLFERLKLKGAILNVNSIGCKNCRPAYVEKLRAELLNVREQLGEDS
                     KRRIETNPLRVLDSKLEHEQAIIEKLPRIAEHLCNDCRAHYDEVKRQLGLRGITYQEN
                     WRLVRGLDYYMRTTFEITAPGLGSQNAVCGGGRYDGLVDLLGGPATTKGIGFAIGTDR
                     L"
     gene            complement(1279..1749)
                     /locus_tag="VG272_01665"
                     /pseudo
     CDS             complement(1279..1749)
                     /locus_tag="VG272_01665"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
     gene            complement(1703..2224)
                     /locus_tag="VG272_01670"
     CDS             complement(1703..2224)
                     /locus_tag="VG272_01670"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HWF12416.1"
                     /translation="MIRSHHGRTPQIAASAYIDPQAVIIGDVSIGEYSSVWPCTVIRG
                     DVNWIRIGSRTNIQDGSILHVYKDTHPLVLADMVTVGHGAILHGCTIESRVLIGMGAI
                     LLNGVRVGENSIVAAGTLVPEGTIIPPGSLFMGHPGKFRRTLTPEDQASIEAYATRYV
                     EYSETYKKEVASD"
     gene            complement(2385..3221)
                     /locus_tag="VG272_01675"
     CDS             complement(2385..3221)
                     /locus_tag="VG272_01675"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HWF12417.1"
                     /translation="MPPPPMPQPPKLPIQGVKNLVAVASGKGGVGKTTVAVNLALALK
                     NMGASVGLLDADVYGPNVPIMLGTDEQPQALSEREILPVMAQGVKMISMGLLNPGDKP
                     MIWRGPMLHSVMQQFLRSVMWGELDYLIIDLPPGTGDVQLSLIQLVPITGAMLVTTPS
                     TVALADVRKAMEMFRQVNVDILGVVENMSFFSCPHCQGRIDVFGHGEGKRIAETYSVP
                     LLGEIEIDPQIRIGGDTGKPVALLGPDAPGAKSIYAMARAVVARLDEKPASARGSVVE
                     ID"
BASE COUNT          791 a          972 c          919 g          840 t
ORIGIN      
        1 aggcggtccg tgccgatggc gaatccgatt ccttttgtgg tggccgggcc gccgagcaag
       61 tcgacgagac catcatagcg cccgccgccg cacaccgcat tttgcgaacc aagtcctggc
      121 gcggtgattt caaatgtcgt gcgcatgtag tagtcgagcc cgcgcacgag ccgccaattt
      181 tcctgatacg tgatgccacg caagccgagc tggcgcttca cttcgtcata gtgcgcgcgg
      241 caatcgttgc aaagatgttc ggcaatgcgc ggcagctttt cgatgatggc ctgctcgtgt
      301 tcaagcttcg agtcgagcac gcgcagcgga ttcgtctcga tgcggcgttt gctgtcctcg
      361 cccaactgtt cacggacgtt gagtaattcc gcgcgcaatt tttctacgta ggcggggcgg
      421 caatttttgc agccgatcga attgacattg aggatcgcgc ccttgagctt caatcgctcg
      481 aagagcctca tcagcatttc gatgacttcc gcgtcgatcc ccggcgcgtc cgaagaaccc
      541 agcacctccg cgccaatttg ataaaactgg cggtagcgcc ccttttgcgg acgctcgcgg
      601 cgaaacattg gccccatgta atacagcttt acattccccg gatgtacgtg cattccatgc
      661 tcgatatacg cgcgcacgac agaggcggtg gcttcagggc gcagagcaag agtatccccg
      721 aaacgaaacg agcgaagggc agttttcagt cctgtcaagc actgagcgaa tccttcttct
      781 gcttgagaat tggaaggatt ttcaagattc atgctgccga agcgattcat tcgttccaag
      841 acttcgggga ttactcgaag tactctctcg ttctcaggcg tccgaggtac ttcacctctt
      901 ttaaaaaatt cagacaggtt tgcatggaaa tcaaggacgc ttcgttcata cagctggaac
      961 cctgaaatat cctcgatgct ttggctcctg attagaagag cggcccgaat gtgctcaaat
     1021 ctaagtagat tgtgatcctc aaatgagtac atctccttcg acacgacatc cgaatcttct
     1081 cctatagaac gggcaaataa gtcaacttgc tcgaaaatcg gcaaacgaat ttcggcgaag
     1141 ccgaaggtgc caaaagtttc tcgcgcagtc tgttcgacgc ggttccacag cgcggattcc
     1201 ggcgggagga tgtcgcgcac gcctttgatg gcctggaatt gttttggttt ttgtggtgta
     1261 gaagtcattt tgaagtggct agtggctagt ggctagcgag ttctccaaag tccgccgtaa
     1321 ggcagcgatc attttttgca attcgctaat taaccccgca acactctcca catcattttt
     1381 caaggaataa ttcatttcca cgctcagtaa taattgcgtc tcaagttcag caagcgagcc
     1441 gctggcatga gagagaaatt gcaaaaattc gcgcgtgccg ttgcgggcct gcccctcagc
     1501 aatattggac ggcactgata cggcagcgcg gcgcatttaa gatataaggc caaatcgttc
     1561 gtccaccggg aattttgcgg taagtccata aacacctttt gcaagcagca tccccttctg
     1621 ccaaacaagc agatccttat aatgctttgc cttctcacga ccgttcagca cgctgtctct
     1681 ctcctgcact agccactcac cactagtcac tagccacttc ctttttgtaa gtctccgaat
     1741 attccacata gcgcgtggcg taggcctcga tgctggcttg atcctcgggt gtcagggtgc
     1801 gtcggaattt tccgggatgg cccatgaaga ggcttcccgg cgggatgatc gttccctcag
     1861 gaaccaatgt gcctgctgct acgattgagt tttcgccgac gcgtacgccg ttcagcaaaa
     1921 ttgctcccat gccgatcagc acacgcgatt cgatggtgca gccatgcaga atggcaccgt
     1981 ggccgaccgt gaccatatcg gcgagtacca gcggatgcgt atctttgtaa acgtgcagga
     2041 tcgacccgtc ctgaatattg gtgcgcgaac cgatgcgaat ccagttcacg tcgccgcgaa
     2101 ttacggtaca cggccaaacg ctggaatatt ccccgatgct gacgtctccg atgatgactg
     2161 cttgtggatc aatataggcc gacgcggcaa tttgcggcgt ccggccatga tggctgcgaa
     2221 tcatgcatgc acctcgtagg aatttaaaca agtgaagcta acacgcgcgt tccggactgg
     2281 caagaaagcg cttttcgtgg catacgtggc ataggcttca gcctgtgggt tttggtcctt
     2341 gccaggacta aattccacag gctgaagcct gtgctactta aaccctagtc gatttccact
     2401 accgagccgc gcgcggaggc cggcttctca tccaggcgcg ccacgaccgc gcgagccatc
     2461 gcataaatac tttttgcgcc gggcgcatcc ggaccaagca gcgcgaccgg ttttccggta
     2521 tcgccgccga tgcgaatttg cggatcgatt tcaatttctc ctagcaacgg cacgctgtat
     2581 gtttctgcga tgcgtttccc ttcaccgtgg ccgaaaacgt cgatgcgtcc ctggcaatgc
     2641 gggcagctga aaaagctcat attctcgacg acgccgagaa tatcgacatt gacctggcgg
     2701 aacatttcca tcgctttgcg cacatcggcc agtgcaacgg tggacggagt ggtgaccagc
     2761 atcgcgccag tgatcggcac aagctgaatc agagaaagct gtacgtcgcc cgtgccgggc
     2821 ggcaaatcaa taatcagata gtcgagttca ccccacatga cgctgcgcag aaattgttgc
     2881 atcacgctgt gtagcatcgg cccgcgccag atcattggct tgtcacccgg attgagcagg
     2941 cccatcgaaa tcattttcac gccctgcgcc atgaccggga gaatctcccg ttcgctgaga
     3001 gcctgtggct gctcgtccgt gccgagcatg attggcacgt tcggcccgta cacgtccgca
     3061 tcgagcaagc cgactgatgc acccatattc tttaacgcaa gggcaaggtt cacggcgacg
     3121 gtagtctttc cgacgcctcc ctttccgctg gcgacggcga caagattctt caccccttgg
     3181 atgggcaatt tcggcggttg aggcatcggt ggcggcggca tggtcaattt ctcctgaaag
     3241 aacagattcc tgtacggctc attttacagc ccttcatttt gaacgagaaa tgagcctacg
     3301 tccactgccg ccagccataa ggcgtgcaaa ctcgtgaaaa tttcgatcca ttaaaaagtt
     3361 ccattcattt aaagaaattt ccaagtcggt aaattagatt caaagtcgtg cacaaccatc
     3421 gtatggggtt aaccatccaa ctgggtgcag ggatattggg gagtggtctt aggtatagat
     3481 agttcttggt ggaattactg aacaaactgg tattgtacat ct
//
LOCUS       DASZMB010000037        12581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_5247820, whole genome shotgun sequence.
ACCESSION   DASZMB010000037 DASZMB010000000
VERSION     DASZMB010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 12581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12581
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5247820"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VG272_01680"
     CDS             complement(<1..1163)
                     /locus_tag="VG272_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12418.1"
                     /translation="MSSYPELKIFDAPGARQVFNVTVPFGTTLTSYSVLTLGAPNLDF
                     SSVPRGTTCPNVVAGTCTIEVQFKPTAFGRRQGALVLNDASGNVLLTVSLNGASNEPM
                     TLIGSGTTSTFAGNGKGGDGGPAIDALLAGPTGIAVDGFGNHYIADRKDNKIRKVSPS
                     GVISTFAGTGSQGYFGDGAPATSATLNGPMSVLVDGAGFVYIADTGNNVVRMVNTSGI
                     ISTYAGQYYAPGTIPPKVCARAINSVGDGCPANQIVLNAPVDLVLCHSQNIHISDQMN
                     NRIRTIIRTTYKTITQVGNGQVGYNGDGELNTKAELNRPTGIAMDADNYIYVADTGNH
                     IIRKTLLDGNTPTPIATIAGIPGRAGNLGDGGPAVSAELNSPRGVQVDAAGDV"
     gene            complement(1613..1688)
                     /locus_tag="VG272_01685"
     tRNA            complement(1613..1688)
                     /locus_tag="VG272_01685"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1653..1655),aa:Thr,seq:cgt)
     gene            complement(1727..2725)
                     /locus_tag="VG272_01690"
     CDS             complement(1727..2725)
                     /locus_tag="VG272_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HWF12419.1"
                     /translation="MSTYDLICIGAGPTGLACAIEAKRAGMLPLVIDKGCLCNSLYHY
                     PVNMVFFTTPELLEIGDLPLTIASEKPTRTEALKYYRKCAEHYELDLRLEHLVERVEG
                     VSGKFIVHTKDEHGNTAQFTGKKIAVATGYYDLPNRMGIPGEDLPHVSHYYTEPHPFW
                     RRNVVVVGGKNSAAEAALDLYRNGANVTLVHRKAELGSTIKYWVRPDIENRIKAGQVK
                     ALFETTVQRFEEGRVIVSNSNGESAIPADQVFALTGYHPDFQFLRSLGIKLDPDTNKP
                     VMDPQSHETNIPGIFVAGVVTGGNHTSEIFIENGRFHGKQIVAALTGIRIDSAAKV"
     gene            complement(2887..6012)
                     /locus_tag="VG272_01695"
     CDS             complement(2887..6012)
                     /locus_tag="VG272_01695"
                     /inference="COORDINATES: protein motif:HMM:NF033617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HWF12420.1"
                     /translation="MHISAPFIRRPVATSLLTVAILLAGCVAFYFLPVSALPHIDFPT
                     ITVRGSLPGASPETMASAVATPLERQFGRIADVTQMTSDSELGNTRITLQFDLDRNIN
                     AAARDVQAAINAAAGQLPPSLPNLPTYRKVNPAETDIMDLTVSSDTLSRGQLYDLCDS
                     FLAQKISQVDGVGLVRISGGSKPSVRVDLNPNAMAHYGIGLEEVKAALAAANANTPKG
                     ALEDQSNRWILSDTDQLLKPDQYPQLIVAYRNRAPVRVQDIAKVSEGLEDVRTSGFAN
                     GKISVSMGVGRQPDANIIETVDKIMALLPELRASIPPTVQLDTNYNRTTTIRASVKDI
                     EFTLLLTVALVVMVIFLFLRNAWATIIPSIAVPLSIAGTFGVMYLLHYNVDNLSLMAL
                     AICTGFVVDDAIVVIENISRYLEEGLSPAEAAMKGSREIGFTVLSMSTSLIAVFIPLL
                     LMGGIIGRLFREFSVTLSAAIIVSLCVSLTTTPMMCARFLKSQKDVKHGKMYQVIERG
                     FQWTAETYAAGLRWVLRHQLFMVAVTGATICLSVYLYVVIPKGFFPQQDTGLMAGGVE
                     GTQDMSFASLQEKLKQFTDIVMADPAVKTLVGEMGGGSENTGQMEVELKPLSQRDASV
                     YQVISRLRPKLAKVPGATLFLRAYQDITVGGRISSSQYQYTLSSENLEDLLQWAPRVE
                     AAMKRIPELGDIDSDQQTRGLQATLVIDRDTASRLGVSPRDIDNTLYDAFGQRQVSTI
                     YTELNQYHVVMEIDDAFRESTDGIRNLYVKSSKGNQVPLSAFTHYETKNTALAVNHQG
                     QFPAVTISFNLGDGVALGDAVTKIEAAVLKMGLPADIHPTFQGNARAFQASLSSQPWL
                     ILAALVAVYIVLGMLYESYIHPITILSTLPSAGVGALLALLAFHTELTVIAVIGIILL
                     IGIVKKNAIMMIDFAIDVQRQGKTPEESIYQACLLRFRPIMMTTVAALFGGLPLALMP
                     GTGSEMRRPLGISIVGGLLVSQALTLYTTPVIYLYMDRLQARLKRRKYSKLVESQIAP
                     PGTTVNELPLT"
     gene            6255..8081
                     /locus_tag="VG272_01700"
     CDS             6255..8081
                     /locus_tag="VG272_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12421.1"
                     /translation="MRHAKTSHRSLYYLSGLRILLAMMCLALLAPGLFAADPDVFPLS
                     KVQPGMKGEAYTIFAGDQIEKFDLVVIGVMPNFLAPKESIILVQLLGPKVEHTGVVAG
                     MSGSPVYFEGKLAGALSLKLGQFNKEPLAGITPIENILSLPKGSPGVTHADGSVPASQ
                     DPDKLEAKFEIPADWASRAGVSGGSFLTPIASPLVFSGFSAVAIRQFEHEFAAYGMAA
                     TQGGSVDARSDDAAIRPGDMVSAVLLEGDMSLNASCTVTTIVDGRVYVCGHPLFGFGN
                     VQMPMARGRVLTTLSSDLESTKIVNVGGTIGSFNQDRVTAVIGSLGASPKLIPIDMTV
                     ATPDGDKKLSFRMISNAKISPILMGIATLNGLVQNSVYGEGTTLHLTGTIDIAGHSSV
                     SLDNLFPPIDSFVPDGLPVSSTVQNLFQRIFTNQFETANIEHVTLRMEAKPERRSMTI
                     EGAWLDKADAEPGDTVTVRVQLRPYRGSPVIRDVKIAIPQQAVRGTSMQILASDSGTL
                     NRMNVVSGSQSRLDSLEQLITVLNRERRNNRLYVTLLGSAPTLLVQDKVMPNLPASEI
                     NLLDQRGGPASSQVIRESAAGEWSLPLDQIVQGSSSLTIQIK"
     gene            8123..10486
                     /locus_tag="VG272_01705"
     CDS             8123..10486
                     /locus_tag="VG272_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12422.1"
                     /translation="MRNLYSSARGHRRAKLIHVAPYGAFLFFAVAMLALAAPALAERT
                     RWWRQSTFEEFDKGTAKGVALRSDGKMFLAPRFAEFSDASLAYLLQVRSDTKGNLYAA
                     GGSNAKVLRIDANGKSTSVFESQELSAQAIALDAAGNLYVGTSPDGKVYKVTPAGQSS
                     VFFDPKTKYIWDLVVDKDGTLYVATGDTGKIFSVAPDGKGQVFYASDETHVRSLALDA
                     NGNLLAGTEPNGRVLRIPKAANNRRAFVLYETSKKEITSLLLAPNGDLYVASVGEKTP
                     PVPGQARPMTTDTSTNTNFVISIGPSGTTSGAAQAGQQNPFTPLPTVNSSSVYRIASD
                     GSPEELWSTRDDVVYSMGLLPGGKLLLGTGNDGAILELEGNHIFSKLAKATSHQVTAI
                     ASGPGGKLFLAAANPGKVFTLGPESESEGTYESQAFDAHIFSHWGRTMWLNENAASGG
                     ANGSRVAVYARSGNTSDPDSNWSDWAGPYTNSTGDKIECPAARFVQWKAVLRGGGANS
                     ANAAPAIDWFSVAYLPKNIAPQVTAIAVQDPGIRVQGLSLPSVGSGTQGSAQLRMPQP
                     PPTTSSSNSSFTVTTISTIGSSGSAHFDPIPQGTSQKGFESVLWTAEDANDDQIEFSV
                     YFRGENETSWKLLKDKLDTKFYSWDTTSMPDGVYYLKIVASDAPSNPTGEGLSSERES
                     DRFVVANTPPSIGQLTADSASAGVRVKFQASAPVSFIARAQYSLDAGDWTLIFPAGGL
                     SDASKENYDFQLQKVLPGEHTVTVRVYDQFENVTSAKTTVRIPSNGN"
     gene            complement(10585..10932)
                     /locus_tag="VG272_01710"
     CDS             complement(10585..10932)
                     /locus_tag="VG272_01710"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWF12423.1"
                     /translation="MSSTDRRTDPRVNACVPMRFRVLDKPEMTEQQGESQNMSQHGIY
                     FLTTAPLQIGMPMELTLRIPHDLGQRISSEVVCVGRVVRVQPIESKEEMLGVGIHIER
                     FETKFQVQEPWAS"
     gene            11044..11184
                     /locus_tag="VG272_01715"
     CDS             11044..11184
                     /locus_tag="VG272_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12424.1"
                     /translation="MVRPGTDTAHAGDTPSDDRATYEAAQSLPADNTGGPYENRTPRL
                     IL"
     gene            11245..12246
                     /locus_tag="VG272_01720"
     CDS             11245..12246
                     /locus_tag="VG272_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWF12425.1"
                     /translation="MSPSDTPHFSGPDLQAIRDAQRRIAPYIEQTQVMISYPLDELAG
                     ALLYFKCENLQLSGSFKLRGAANAVFSLSEAEAARGVVTHSSGNHGAALALAAQMRGI
                     RAWVVMPRNAPQVKVRAVEAYGADISFCEPTLHAREAGAAELLKSTGGTLIHPFDDDR
                     VIAGQGTAALELLEEIPDLDIICAPVSGGGLLSGTAIAAKSLRPEIQVIGCEPNNADD
                     AARSMASGKIEPASIQFTLADGLRATLAPRTFAILRERVDRILLATEQEIVKTMHLIT
                     EHLRITVEPSGAVAASPALHHQIEGDDLRIGIILSGGNLDPDTLPANTESETRRQRE"
BASE COUNT         2824 a         3575 c         3372 g         2810 t
ORIGIN      
        1 aaaacatctc cagcggcatc cacttgcacc ccccgggggc tgttcagctc agcactcact
       61 gcgggccctc catcgccaag gttgccggcg cggcctggga tcccggcaat tgtcgcaatc
      121 ggggtcgggg tatttccatc caataaagtc ttgcgaatga tgtgattccc ggtgtccgcg
      181 acgtagatgt agttatcggc gtccatggcg atacccgtgg gacggttgag ctccgccttg
      241 gtattgagtt cgccgtcgcc gttatagccg acttggccgt ttcccacttg cgtaatagtc
      301 ttgtaggttg tcctgataat cgtgcggatc ctattgttca tctggtctga gatgtgaatg
      361 ttttgagaat ggcacagcac caggtccacg ggcgcattca ggacaatctg attcgctgga
      421 catccgtcgc ctacggaatt tatagcccgg gcgcatacct tcggaggaat tgtgccgggc
      481 gcataatact gacctgcata tgtcgagata attcccgagg tgttcaccat gcggacgacg
      541 ttgtttcctg tatcagcgat gtaaacaaat cctgcgccat ccacgagtac ggacatgggg
      601 ccattaagtg ttgcactggt cgccggtgcg ccgtcgccga agtagccctg gctgcctgtt
      661 cctgcaaatg tcgatataac tccggacggg ctgaccttgc ggattttgtt gtccttgcgg
      721 tccgcgatgt aatgattgcc aaatccatcc accgctatgc ccgtcggacc agctagaagt
      781 gcgtcgatgg ccgggccgcc gtcgcccccc ttgccattac ctgcaaacgt tgaagtggta
      841 cctgagccaa tcagcgtcat cggctcattc gaggcaccgt ttaatgaaac ggtaagcaac
      901 acatttccgc ttgcgtcgtt aagtaccagg gctccctgcc gcctgccgaa agcggtcggc
      961 ttgaactgga cttcgatcgt gcatgtgcca gcaacgacat tcgggcaggt ggtgccacgc
     1021 ggaaccgaac tgaaatcgag gttcggcgcc ccaagcgtca aaacggaata gcttgtgaga
     1081 gtcgtgccaa acggaactgt aacattgaaa acctgccttg cacctggggc gtcaaaaatt
     1141 ttcagttcgg ggtatgaact caccttcgtc gtcaatcgcg ttgaaatgga caaaaggcag
     1201 gcaacacata agaggatcga gagtcgaagc gcaaatgcag agcgagaatg cgacacgttt
     1261 acactcaccc caatgtgatc cgccggctaa gtttccgtcg aatcgcagtc gggcttcaat
     1321 acagtcaaga ctctagaacc ttaggcattt ccctctaatg ctacttggcg aaatggaagg
     1381 cgcacgtcag ctgactaaaa ataaaataca ccatcagcca ctgaacgcgg agattgtcgg
     1441 tcgctcgtca cgtgtcaaga aagatgctcg agcattattc ccgtgttcgc aacgtacgca
     1501 aagcgtacag ctctggacgg gttgaaaccg gctctccagg tggctgtgca ccaaaacgtg
     1561 caccaactaa cgagtaatga aacagcgcct gtttctaagt tgttgaatga gttggagccg
     1621 aagtccggac ttgaaccgga gacctgtcga ttacgaatcg actgctctac caactgagct
     1681 acttcggctt ggtgcaaaac actgtagcat tgtgcggaaa aaagtgtcaa accttagcgg
     1741 ccgaatctat acggatgcct gtcaaagcag caacaatctg ttttccgtgg aagcgcccat
     1801 tttcgatgaa aatttcgctg gtgtgattgc cgccggttac tactccggcc acgaagattc
     1861 cgggaatatt ggtttcgtgg gattgcgggt ccatgaccgg cttgttggtg tccgggtcca
     1921 gcttgattcc cagggaacga aggaactgaa agtcagggtg gtatcccgtg agggcgaaga
     1981 cttgatctgc aggtatcgcc gattcgccat tactattgga aacaatcact ctgccttcct
     2041 caaaacgctg aaccgttgtt tcgaacaatg cctttacttg tccggctttg atgcgatttt
     2101 cgatgtccgg gcgcacccaa tattttatgg tcgagccgag ctccgctttg cggtgaacca
     2161 gcgtaacgtt tgcgccgttg cgatatagat ccagcgcggc ttctgccgcg gagtttttac
     2221 cgcccacgac gacgacattt cgccgccaga atggatgcgg ctcggtgtag taatgtgaga
     2281 cgtgaggcaa atcttctccc ggtatgccca tgcgattcgg gagatcgtag taaccagtgg
     2341 ccacggcaat ctttttgccc gtaaattgcg cagtgttccc gtgttcatcc ttcgtgtgta
     2401 ctatgaattt cccgctgaca ccttccacgc gctcgacaag atgttccagt cgcaggtcca
     2461 gctcataatg ttccgcgcat ttgcggtaat actttagtgc ctcggtgcgc gtgggctttt
     2521 cagacgcaat ggtcagcggc aaatcgccaa tttccagcag ctcaggtgtc gtgaagaaca
     2581 ccatgttgac ggggtagtgg tagagcgaat tgcacagaca gcctttgtcg attacaagtg
     2641 gaagcatgcc tgcacgcttc gcttcaattg cgcaggccaa gcccgtcggc ccagcgccaa
     2701 tacaaatcag gtcatacgtg ctcataaatt aagtttgcac tggcgagaat tgttttgcaa
     2761 ccggtgcgct ccgtgtggca taggctttag cccgtgggtt ttgtacctgc gaagacccaa
     2821 gtccacaggc tgaagcctgt gccacgtaaa gcagctccaa ttattacaat tggagaagga
     2881 caccgactaa gtcagcggca attcattaac cgtcgtgcct ggcggtgcga tctggctttc
     2941 aactaacttg gaatacttgc ggcgcttcaa gcgagcttgc aggcgatcca tatacaaata
     3001 aatcacagga gtcgtataca gcgtgagcgc ctggctgacc agcaggccgc ctacgatgct
     3061 aattccaagc ggccggcgca tctccgaacc tgttcctggc atcaaggcga gcggcaagcc
     3121 tccaaacaac gctgcaaccg tggtcatcat gatgggacgg aagcgcagta agcaagcctg
     3181 atagatcgat tcctctggag tttttccctg ccgctgcaca tcaatcgcga aatcgatcat
     3241 cataatggcg ttctttttca caattccgat cagcaaaata attccgataa cggcaatgac
     3301 cgttaattcc gtgtggaacg cgagcaaggc tagaagcgcg ccgacgcccg ccgacggcag
     3361 cgtggaaaga atggtgatcg gatggatgta gctctcataa agcattccca gaacaatgta
     3421 cacggcgacg agcgcggcga gaatcagcca cggctggctc gagagtgaag cctgaaaggc
     3481 gcgcgcattc ccctgaaacg tgggatgaat gtccgcggga agtcccattt tcaaaaccgc
     3541 ggcttcaatc ttggtcacgg cgtcgccgag agcgacgccg tccccaagat taaatgaaat
     3601 cgtgaccgcg ggaaattggc cctggtgatt taccgccagc gccgtattct tcgtctcata
     3661 atgagtgaag gcgctgagcg gcacctgatt cccttttgac gatttcacgt acagattgcg
     3721 aattccgtcc gtgctttcac ggaacgcgtc gtcgatttcc atcaccacgt gatactggtt
     3781 cagttccgta tagattgtgg agacctggcg ctgtccgaat gcgtcgtaaa gcgtgttgtc
     3841 gatatcgcgc ggtgaaactc cgaggcgcga ggcggtgtca cggtcaatca ctagcgttgc
     3901 ctggaggccg cgtgtttgct ggtcgctgtc gatgtcgccc agctccggaa tgcgcttcat
     3961 cgcagcttcg acgcgcggag cccactgcaa caaatcttca agattttcgc tggaaagcgt
     4021 gtactggtat tgcgaactgc tgatgcgccc gcccaccgta atgtcctggt aagcgcgaag
     4081 aaacaatgtg gcgccgggaa cttttgcaag tttaggacgc aacctcgaaa tcacctggta
     4141 gaccgaagcg tcccgctggc tcagcggctt caattcaact tccatctgtc cggtgttctc
     4201 ggaccctcca cccatttctc ctacgagggt ttttaccgcg ggatcggcca tcacgatatc
     4261 cgtaaattgt ttcagctttt cctgcaacga agcgaaggac atatcctgag tgccttcaac
     4321 tcctccggcc atcagtccgg tatcctgctg cggaaaaaaa cccttgggta tcactacata
     4381 gaggtacacg ctcaggcaaa tggttgcacc cgtcacggcg accatgaata actgatgccg
     4441 tagcacccat cgcaacccag ccgcataggt ttccgcagtc cactgaaacc ctctctcaat
     4501 tacctgatac atctttccgt gtttgacgtc tttttgcgat ttcaagaatc gcgcgcacat
     4561 catcggcgtc gtcgtgagcg acacgcaaag tgaaacgatg atggcggcgc tcagcgtgac
     4621 ggagaactcg cgaaaaagcc taccgataat cccgcccatc aagagcagcg gaatgaatac
     4681 ggcaatcagc gacgtgctca tggaaagtac ggtgaacccg atttcgcgcg agcctttcat
     4741 tgcggcttct gccggagaaa ggccctcctc cagatatcgc gaaatgtttt cgatcaccac
     4801 gatggcatcg tccaccacga agcccgtgca aatcgccaaa gccattaggg aaaggttgtc
     4861 cacattgtag tgcagcagat acataacgcc gaaggtcccg gcgatcgaca gtggcaccgc
     4921 gatgctgggg atgatggtgg cccatgcgtt gcgcaggaaa agaaaaatca ccatcaccac
     4981 aagcgccacc gtgagcagca gcgtaaattc gatgtctttc acggaagcgc gaatcgtcgt
     5041 cgtgcggttg taattcgtat ccaattgaac cgtgggcgga atggacgctc tcagctccgg
     5101 cagcagagcc attattttgt ctaccgtttc gatgatattc gcgtcgggct gccttccgac
     5161 gcccatggaa acggaaattt ttccgttcgc aaatccggaa gttcggacgt cctcgagccc
     5221 ctcgctgact ttcgcgatgt cctgcactcg gacgggagcg cggttgcggt aggccacgat
     5281 cagctgcggg tactggtccg gcttcaaaag ctgatcggta tcactcaaaa tccagcgatt
     5341 ggattgatct tccagcgcgc ccttgggagt gtttgcattc gccgccgcga gtgctgcttt
     5401 tacttcttct agcccaattc cgtaatgcgc catcgcgttc gggttcagat ccacgcgtac
     5461 cgacggcttt gacccgcccg aaatacgcac gagccccacg ccgtcaacct gcgaaatctt
     5521 ttgcgccaga aaagagtcgc acaaatcgta aagctgacct cgtgaaagcg tgtcagagga
     5581 aaccgtcaag tccataatgt cagtttccgc ggggtttact ttccggtacg tgggcagatt
     5641 cggaagactg gggggcagtt ggccggcggc ggcgttaatt gccgcctgta catccctggc
     5701 ggccgcgttg atgttccgat ctagatcgaa ttgcagtgtg attcgcgtgt tacccagttc
     5761 actgtcagac gtcatctgcg tcacgtccgc tatccgccca aattgtcttt cgagcggcgt
     5821 tgccacggca gacgccattg tttcgggact cgcccccggc aggctgcctc gtacagtaat
     5881 cgtggggaaa tcgatgtgag gcaaagcgga cacgggcaaa aaataaaacg caacgcagcc
     5941 cgcgagcaga atggcaaccg tcaacaaaga agttgcaact ggccgtcgaa taaatggggc
     6001 cgaaatatgc attttgattt attcagtcac aagccactcg ccggatccgg gagaaccgaa
     6061 atccatacac gcgactttac cactgcaata ctaatcccga tcatctgaat gtttcctgaa
     6121 tgcggtgcgc gattcgagtt tttcagtcgc atccgatcgg aaaatttccg caaaacccgc
     6181 tggtaattcc tgcgccactc gagagttgtc ccaagattga ttccggcgga gggcgcgcgt
     6241 atcataggag attaatgcgt cacgccaaaa ccagccaccg cagtttgtat tacctttccg
     6301 gcctgcgaat tctgctcgcg atgatgtgcc tggcgctgct cgcgcccgga ttgttcgccg
     6361 ccgacccaga tgttttcccg ctctccaaag ttcaaccggg aatgaagggg gaggcctata
     6421 ccatttttgc cggcgatcag attgaaaaat tcgacctggt ggtgatcggc gtcatgccca
     6481 acttcctggc gccaaaggaa tcgatcatcc tcgtgcaatt gctggggccg aaagtggagc
     6541 acacgggagt ggtcgcgggc atgagcggca gtcccgttta ttttgagggc aagctagcag
     6601 gcgcattgtc cctaaagctt gggcagttta ataaagagcc gctggccggc atcacgccca
     6661 tcgaaaacat tttgagcctg ccaaaaggtt cgcccggcgt tacgcacgcg gacggttccg
     6721 ttcccgcttc ccaagatccc gacaagttgg aagcgaaatt tgaaattccc gcggattggg
     6781 cttcgcgtgc gggggtttcc ggcggaagtt ttctgacgcc catcgcttct ccgctggtat
     6841 tttccggatt cagcgccgtg gccattcgtc agtttgagca cgaattcgcc gcctacggaa
     6901 tggcggccac gcagggaggc agcgttgacg cccgctccga cgacgctgcg attcgccccg
     6961 gagacatggt cagcgccgtg ctcctcgaag gcgacatgtc cctgaacgca tcctgcacgg
     7021 tcacaacgat tgtcgacggc cgcgtatacg tctgcgggca cccgctattt ggctttggca
     7081 atgtgcagat gcccatggct cgcgggcgtg ttctcacaac gcttagctcg gacctcgaat
     7141 ccacaaaaat tgtgaacgtg ggcggtacca tcggatcatt caaccaggat cgcgtaaccg
     7201 ccgtcatcgg cagcctcggc gcttcgccga agttaatccc catcgacatg acggtggcga
     7261 cgcccgacgg cgacaaaaag ctctctttcc gcatgatttc caacgcgaag atcagcccca
     7321 ttctcatggg tatcgccacg ctcaacggtt tagtgcagaa cagcgtctat ggtgaaggga
     7381 caaccttgca cctcacggga accattgaca tcgcgggcca ttcgagcgtg agcctcgaca
     7441 atcttttccc gccgatcgat tcttttgttc cggatggttt gcccgtctcc agcaccgtgc
     7501 aaaatttgtt tcaaagaatt ttcaccaacc aatttgaaac cgcgaatatc gagcacgtga
     7561 cgctgcgcat ggaagccaaa cctgagcgcc gctcgatgac cattgaaggc gcgtggctgg
     7621 acaaggccga tgcagagccc ggcgatacgg ttactgtacg cgtacaattg cggccctacc
     7681 gtgggtctcc ggtgatccgc gacgtgaaga tcgccattcc ccagcaggca gtgcgcggca
     7741 cgtcgatgca aattctcgcc agtgattccg gaacgctgaa tcgcatgaac gtcgtctccg
     7801 gctcacaatc gcggctggac agtctggaac agctcatcac cgttttaaat cgagagcgga
     7861 gaaataaccg cctctacgtc acgcttctcg gctctgcgcc gacgcttctg gtgcaggaca
     7921 aggtgatgcc aaacctgcct gcctctgaga tcaatcttct ggaccagcgc ggcggcccgg
     7981 caagctctca ggtgattcgg gaatcggcgg cgggagaatg gtcgcttccg ctcgaccaga
     8041 tcgttcaggg atcttcatct cttacgattc aaatcaagta agcagcaggc tggcgagtgt
     8101 cgccggttcc tttaggagtc ttatgcgcaa tttatattcc tcggctcgcg gacatcgccg
     8161 cgcgaaatta attcatgtcg ctccgtacgg ggcgtttctt ttttttgccg tcgccatgct
     8221 cgccctggca gcgcccgcac tggccgaacg cacgcggtgg tggcgccaat ccacgttcga
     8281 agaattcgac aaaggaacgg ccaaaggggt tgcgctgcgc agtgatggga aaatgtttct
     8341 tgctccacga tttgctgaat tttccgatgc gagcctcgcc tacctcctgc aagtccgctc
     8401 cgacaccaag ggaaatctgt acgcggcagg cggatcgaac gccaaagttc tgcgcatcga
     8461 cgccaatgga aaatcgacca gcgttttcga atcgcaggaa ttatccgcgc aggccatcgc
     8521 gctggatgct gccggcaatt tatacgtcgg cacatctccc gacggcaaag tgtacaaggt
     8581 aacgcctgcg ggccaatcct ctgtattctt cgatccgaaa acaaaataca tctgggatct
     8641 ggttgtggat aaagacggaa ctctttacgt tgcgaccggc gacaccggca agattttttc
     8701 tgttgcgccg gacggtaagg gacaggtttt ttacgcaagc gatgaaactc acgttcgctc
     8761 tctcgcgctg gacgcgaatg gaaatctttt ggcgggaact gagccaaacg ggcgcgtctt
     8821 gcgcattccc aaagccgcaa acaatcgccg cgccttcgtg ctgtacgaaa cgtccaagaa
     8881 agaaatcact tcgctgctcc tggcgccaaa cggcgatcta tatgtagctt cggtcggaga
     8941 aaaaactccc cccgttcccg gacaggcccg gcccatgacg acggatactt ctacgaacac
     9001 caatttcgtg atttccatcg gaccatcggg aaccacctcc ggcgctgcac aggctggcca
     9061 gcagaatccg ttcactcccc tgcccaccgt aaattccagt tccgtgtatc gcatcgcctc
     9121 cgacggctct ccggaagaat tgtggagcac gcgggacgat gtggtgtatt cgatgggcct
     9181 gcttcccggc ggaaaactct tgctaggaac cggaaacgac ggcgcgatcc tcgagctcga
     9241 aggcaatcac attttttcaa agttggccaa agccacttcg caccaggtca ctgcgatcgc
     9301 ctcgggcccc ggcgggaaat tatttttagc cgcagccaac cccggaaaag ttttcacgtt
     9361 gggaccggaa agcgagtctg agggaacgta cgaatcgcag gcctttgacg cgcatatctt
     9421 ttcgcactgg ggccgcacca tgtggctcaa cgaaaacgcg gcgtccggcg gcgcgaacgg
     9481 ttcgcgcgtt gccgtctatg cgcgctctgg aaacacttcc gaccctgact ccaactggag
     9541 cgattgggcc ggcccctaca caaactcaac gggcgacaag atcgaatgcc ctgctgctcg
     9601 cttcgtgcaa tggaaggcag ttttgcgagg aggcggcgca aacagcgcaa atgctgcgcc
     9661 cgcgattgac tggttcagtg ttgcgtacct tccaaaaaat attgcccccc aagtgacagc
     9721 gatcgccgtt caggatcccg gaattcgcgt gcagggattg tcgctaccga gcgtaggcag
     9781 cggaacgcag ggttcagcgc agttgcgcat gccgcaacct ccgcccacca cctcgtcgtc
     9841 caactcttcc ttcaccgtga ccacgatttc cacaatagga tcctccggct ccgcgcactt
     9901 cgatccgatt ccgcagggca cctcgcaaaa aggttttgaa tccgtgctgt ggactgcaga
     9961 agacgcgaat gatgaccaga tagaattttc tgtctatttt cgcggtgaaa atgagacatc
    10021 ctggaaactg ttgaaagaca agctggacac taaattttat tcgtgggaca ccacgtccat
    10081 gccggacggc gtgtactatt taaaaatcgt cgcgagcgat gccccgtcca atccgacggg
    10141 cgaaggcctt tcttcggagc gtgaatctga tcgctttgta gtggccaaca ctccgccgtc
    10201 gatcgggcag ctaacggcag actcggcaag cgcgggcgtt cgcgtgaaat ttcaggccag
    10261 cgctcccgta agttttatcg cgcgcgcgca atacagtctg gatgcgggag attggacgct
    10321 gatttttccg gcgggaggat tgagtgatgc atccaaggaa aattacgatt tccaactgca
    10381 aaaagttttg cccggagagc acacagtgac ggttcgcgtt tacgaccagt ttgaaaacgt
    10441 gaccagcgca aaaacaactg tgcgaatccc ctccaacgga aactgatttt taagtggcat
    10501 aggcttcacc ctgtgaattc cgtccttgca gggactaaac cccacaggct aaagcttatg
    10561 ccacaaaatt ccgttccttg ggccctaact cgcccacggc tcctgcacct ggaatttagt
    10621 ttcaaaccgc tcaatatgaa tgccaactcc taacatttcc tctttacttt caatcggctg
    10681 cacgcgcacg actcggccga cgcacaccac ttcgctcgat attcgttgcc ccaggtcgtg
    10741 cggaatgcgt agcgtcaact ccataggcat tccaatttgt aacggcgcgg tcgtcaaaaa
    10801 ataaattcca tgctgcgaca tgttctgcga ctccccctgt tgctccgtca tctcaggttt
    10861 gtccagcact cggaacctca tgggaacgca ggcattcacg cgcggatccg tccgacgatc
    10921 agtcgagctc atggtttcct ccgggacgct tggaaaatag cgcgaaaact aatttggaag
    10981 ggtgtaggaa ttctagcgcg tggtgttgcg gaagcaagag cattccgcct taaacaggaa
    11041 tgggtggttc gacctggaac agacacggcg cacgcgggcg acacacccag cgacgaccgc
    11101 gcgacttacg aagcggcgca gtcgctgccg gccgataaca cgggcggccc ctacgagaac
    11161 cggactccgc gccttatttt atgaatggat ttgcgacagg ccgaatgact tgtgacataa
    11221 tgcgccgtcc gcgaggtttc taaaatgagc ccatccgaca ccccgcattt ttctgggcct
    11281 gaccttcagg ccatccgcga cgcacagcga cgcattgctc cctacatcga gcaaacgcag
    11341 gtcatgattt cttatccgct cgatgagctc gcgggcgcgc tactttattt taaatgcgaa
    11401 aacttgcagc tttctggatc gttcaagctt cgcggagcgg cgaacgccgt cttttcgctc
    11461 agcgaagcag aagcggcgcg cggggtcgta acccactcgt ccggaaatca tggcgcggca
    11521 ttggcactcg ctgcgcagat gcgcggaatt cgcgcgtggg tcgtcatgcc ccgcaacgcg
    11581 ccgcaagtga aggtgcgcgc ggtggaagcg tatggcgccg acatttcatt ctgcgaaccg
    11641 acgctccacg cgcgcgaagc cggcgccgct gaattactaa aatccacagg cggcacgctc
    11701 attcatccct ttgacgatga ccgcgtaatc gccgggcaag gcaccgccgc gctcgaactg
    11761 ctcgaagaaa ttccggacct cgatatcatt tgcgcgccag tgtcgggcgg cggccttctt
    11821 tccggcacgg ccattgcagc caagtccctg cgtcccgaaa ttcaagtgat cggctgcgaa
    11881 ccgaataatg cggatgatgc tgcgcggtcc atggcttcag ggaagattga gccagcttcg
    11941 atccagttca ctcttgcaga cggcctgcgc gccacgcttg cccctcgcac gttcgccatt
    12001 ttgcgggaac gcgtcgatcg gatccttctc gccacggagc aggaaattgt aaaaaccatg
    12061 cacctgatta cagagcacct gcgaatcacg gtcgagcctt ccggcgccgt cgccgcatct
    12121 cccgcgctgc accatcaaat cgaaggagac gacctgcgaa ttggaatcat tctttccggc
    12181 ggaaatctag acccggacac attgccagcg aatacggaaa gcgaaactcg ccgccagcga
    12241 gaatgatcgg gattcgtgag tcgtaatttg tgattcgcga ttcgtaatcg gttatttaga
    12301 actccagatt tggagttcag tttgaagtct cggtcttgac atcttcgtga agatttgaaa
    12361 tttcaatttg agccggtgat tgttccccgc cacgcccttt tccaacactt aaatcttttc
    12421 ccccgctttc cgcaatgtct cgattcgcac cgcacattag aaattactca cgtcgttcgg
    12481 aatgaccgga tcggttaaaa aatggcatga taatctttca tcattggacc gtcaccgtaa
    12541 cagtttgggt tggtgacgca acgtaattgc cgtcgccgct a
//
LOCUS       DASZMB010000038         7286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_26118, whole genome shotgun sequence.
ACCESSION   DASZMB010000038 DASZMB010000000
VERSION     DASZMB010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26118"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1225
                     /locus_tag="VG272_01725"
     CDS             65..1225
                     /locus_tag="VG272_01725"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWF12426.1"
                     /translation="MRSEKVKLDPSRQSVEAQDFGSAMRRRVVGQEDGVRAVTDLYQV
                     FRAGMCSPGRPVGNVLFLGPTGSGKTRIVEAAAEILFGNPRAVIKVDCAEFQHSHEIA
                     KLVGSPPGYLGHRETHPLITQESLAQYHTDKLKLSFLLFDEIEKASDALWQLLLGILD
                     KATLTLGDNRRVDLSQTMIFLTSNLGGGEITELMTGGMGFVQPSDKLEEKLDLKVEKT
                     AQEAAKKKFSPEFMNRLDKVVVFHPLRHEQLEQILEIELGMVQQRVLDTGRGQFLFRA
                     TSAARRFLLQEGTDIKYGARHLKRAIEKFVVYPLANLLATEQIRFGDMIVVDWDDADQ
                     SIAFHREGQNAFLPANPRRQARTAAVRGAVSTMGEGKIRVTDMELEPLLTNR"
     gene            complement(1602..3122)
                     /gene="gcvPB"
                     /locus_tag="VG272_01730"
     CDS             complement(1602..3122)
                     /gene="gcvPB"
                     /locus_tag="VG272_01730"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HWF12427.1"
                     /translation="MKTEEIKLTDRIKTVARHISRNEGLIFEESAPGKRAFELPPLDV
                     PAVDPRTALGSDFVRESIDGFPEVSEIEVVRHFTRLSTWNYAIDLGMYPLGSCTMKYN
                     PRVNEFVARLDGIATEHPYQPQELSQGCLKILQLLLNCLAEITGMDSGTLEPAAGAQG
                     ELTGLLLIRAYLEDTQGHPRKIVLIPDSAHGTNPATAVIAGYDVLNVPSDERGQIDIA
                     RLKEIVNGDVAAMMVTNPSTLGVFEQNIAEAAHILHEYGALLYMDGANMNALAGVARP
                     GDFGVDVMHLNLHKTFSTPHGGGGPGAGPVMVKKLLEPYLPIPVLVEKNGQLNLENRK
                     PKTIGRVRAFGGNFGVLVRALAYTLAYGPGIRQATEDAVLNANYIRKKLENVFDLPFN
                     SPSMHEVVFSDARQKKNGVNTMDIAKRLIDYGFHPYTTAFPLIVPGAMMIEPTESESK
                     QECDLFVDAMRSIAEEAATDPEIVKHAPHSTKVGRLDEVGAARKPVLRWKPKTQSA"
     gene            complement(3178..4524)
                     /gene="gcvPA"
                     /locus_tag="VG272_01735"
     CDS             complement(3178..4524)
                     /gene="gcvPA"
                     /locus_tag="VG272_01735"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HWF12428.1"
                     /translation="MRYLPKSTSERREMLDAVEVPSIETLFESIPEQFRLKEPLNLPA
                     AMSEQEIIEYFRARAKENSVGYTSFLGAGVYNHLRSVVTDALIQRGEFLTSYTPYQAE
                     ISQGTLQAIFEFQTLMCQLTGMEVANASMWDGSTATTESVLMAERLTGRHRVIVARSL
                     HPEYRQVLETYTRNLGLRINEIGFTSSGQVDATRLENALTSETAAVVVQSPNFLGVLE
                     QMPSLAALAHSKGALLVVSIAEGVSLGAVRPPAEADIVAMEGQSFGLAPSYGGPYAGV
                     IATRDKFVRQMPGRLAGETVDSSGKRGYVLTLATREQHIRREKATSNICTNQALCALA
                     VNIHLCLLGKEGLKELAAQNLAKARFALDELEKIRGVRRAFTGPFFNEFVVEFPRSVK
                     MVNAELLKHKIIGPLPVGSYFSDLTKRGLFCVTETISRTEIERMADAIRDILSKPI"
     gene            complement(4524..4916)
                     /gene="gcvH"
                     /locus_tag="VG272_01740"
     CDS             complement(4524..4916)
                     /gene="gcvH"
                     /locus_tag="VG272_01740"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HWF12429.1"
                     /translation="MYPNDYRYTKDHEWIKVSGDTGTIGITDYAQHELGDVVFVEMPA
                     VGSKVSAGKVFGTVESVKAVSEIFAPVSGEVTETNATLTGTPETLNSDPHGSAWLIKI
                     KLANPSEVSGLMDAAAYQAFISEKEASA"
     gene            complement(5076..6185)
                     /gene="gcvT"
                     /locus_tag="VG272_01745"
     CDS             complement(5076..6185)
                     /gene="gcvT"
                     /locus_tag="VG272_01745"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HWF12430.1"
                     /translation="MTSPSTTHLRKTALNAVHRRLKAKMVNFGGWDMPVEYSGIVAEH
                     MATRTAAGLFDVSHMGEIEVRGPHALELVQHVTCNDASKLVIGQAQYSGLMTAQGTFV
                     DDLLVHKISDTHYFLCVNASNQDQDFEHIRAHNGAGAQIENAGERYAQLAIQGPKALG
                     ILQRFTQVPLEPIKYYHFAFGKADGVDCIIARTGYTGEDGFEIYFAPEHSEKLWATLL
                     DAGSGHGLLPCGLGARNTLRLEASMCLYGHEIDDTTTPWEAGLGWICKPNKSEFLGRD
                     ILVKQKEKGVDRTLVGIEMQDRLIARDGCVVSFGNEPVGRVTSGSPAPFLKKNIGMAY
                     VPTASKTPGTEISIGIRANQAPAKIVPMPFYKRTK"
     gene            6688..7170
                     /locus_tag="VG272_01750"
     CDS             6688..7170
                     /locus_tag="VG272_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12431.1"
                     /translation="MANTTVRIAGKPAKSVWFNPMTDEPIRTVFHLDKDSRLVSVFCS
                     AVEHQAAHAGLEGGAGVQLAQAAGDVCREAISQGGGDTGIEVTLDTFQDRVEVAIHYR
                     GQGLPTDGTGSPVVTGTKSKGAESLNGQELLSRVDRVNCKADEGIARVTLVKYLPAHH
                     "
BASE COUNT         1595 a         1867 c         2157 g         1667 t
ORIGIN      
        1 gccataagcc tatccaggtg gacagcaaat tcaccatcgg cgactgaaaa ataacggggg
       61 atttatgagg tcagaaaaag tgaaactcga tccgagccgt cagagtgtgg aagcacagga
      121 tttcggatcc gcaatgagaa gacgtgtggt tggacaagag gatggcgttc gagcggtcac
      181 ggatctttat caagtgtttc gcgctggcat gtgctcgccg gggcgaccgg tcgggaatgt
      241 tttgtttctt ggaccaacag gatcaggcaa gacgcgcatt gtggaagcgg cagcggaaat
      301 cttatttggc aacccgcgcg cggtgatcaa ggtggattgt gcggagtttc agcattcgca
      361 cgaaatcgcc aagctggttg gctctccccc aggctatctg gggcatcgcg agacgcaccc
      421 gctcatcaca caggaatcgc tggcgcaata tcataccgac aaactcaagt tgagttttct
      481 gctgttcgac gaaattgaaa aggcatccga cgccctttgg caattgctgc tgggcattct
      541 ggataaagct acgctgacgc tgggcgataa ccggcgcgtg gacctgtcgc agacgatgat
      601 ttttctgacc tcgaacctgg gcggcggaga gatcaccgaa ctgatgacgg gtggcatggg
      661 ttttgtgcag ccgagcgaca agctggaaga aaagctggac ctgaaagtgg agaaaacggc
      721 gcaggaagcg gcgaagaaga aattttcgcc ggagttcatg aaccgcctgg ataaggtggt
      781 ggtgtttcac cctttgcggc acgaacagct ggagcagata ctggaaattg agttagggat
      841 ggtgcagcag cgcgtgttgg atacggggcg cgggcagttc ctgttccgag cgacgagtgc
      901 ggcgaggaga tttttgttgc aagagggaac ggatatcaag tacggagcgc ggcatttgaa
      961 gcgagcgatt gagaagtttg tggtgtatcc gctggcgaac ctgttggcta ccgaacagat
     1021 tcgtttcgga gacatgatcg tggtggactg ggacgacgcg gatcaatcga ttgcttttca
     1081 tcgcgaaggg cagaacgcat tccttccagc aaacccgcga cgacaggcac gcacggcggc
     1141 cgtgcgtgga gccgtcagca cgatgggaga gggaaaaatc agagtcactg acatggaact
     1201 ggaaccactt ttgacgaata ggtgaggggt caacggaacg ggctttcgtc tttggttttc
     1261 ttgggaaaaa gaaaaaccaa atcgacggaa gcccgtccca cctacaattg catgttatga
     1321 atcactgtca gcgaattaat aaatgggcag gaacgtggca taggctccag cgtgaagctt
     1381 ttggccttgc gctgactaac tccacagcct gaagagtcta tgtgagaact gcaaaattga
     1441 cgcttcagcg gctaaagccg cactcatttt ccaggacata tgtcgtggct gaagccacga
     1501 cccacaaaga ttctgcagtt ctcacacaga ctcttcaggg tgtcgagttt tggccttgcg
     1561 ctgactaacc ccacaggctg aagcctatgc catgaaaaca tttatgcgga ttgcgttttt
     1621 ggtttccagc gcagaacggg tttacgcgcg gcgcccactt catccaagcg gccaaccttg
     1681 gtggaatgcg gcgcatgttt cacaatctcc ggatcagtcg ccgcttcttc ggcgatggaa
     1741 cgcatcgcgt ccacgaataa atcgcactcc tgctttgatt cagactccgt aggctcaatc
     1801 atcatcgcgc cgggcacgat cagcggaaat gcggtggtgt agggatgaaa gccgtagtcg
     1861 atcaagcgct tggcgatgtc catggtgttc acgccatttt ttttctggcg cgcatcgctg
     1921 aaaacaactt cgtgcatgga tggcgagttg aacggcaggt cgaacacgtt ttcgagtttt
     1981 ttacgaatgt aattggcgtt gagcaccgcg tcttccgtgg cttgacgtat tcccgggcca
     2041 taggccagcg tgtaggcaag ggcgcgaacg agcaccccaa aatttccgcc aaacgcgcgt
     2101 actcttccga ttgttttcgg tttgcgattt tccagattca actgcccgtt cttttccacc
     2161 agcactggaa tcggcagata tggctccagt agttttttaa ccatcacggg acccgcgccc
     2221 gggccgccgc caccgtgcgg agtggaaaat gttttgtgca ggttgaggtg cattacatcc
     2281 acgccgaaat cgccgggccg cgcgacacct gccagcgcgt tcatgtttgc gccgtccatg
     2341 tacaagagcg cgccgtactc gtgcagtata tgcgcggctt ccgcgatgtt ttgctcgaat
     2401 acgcccagtg tcgatggatt tgtgaccatc atcgcagcca cgtcgccatt cacgatttcc
     2461 ttcaagcggg cgatatcaat ttggccgcgt tcatcggacg gcacatttag gacatcgtat
     2521 ccggcgatca cggcggttgc cggattcgtg ccgtgcgcgg agtccgggat taaaacaatt
     2581 ttgcgcggat ggccttgcgt gtcttcgagg taagcgcgaa tcaacaacag gcccgtgagt
     2641 tcgccttgcg cgcccgcggc aggttccagc gtgcccgaat ccatgcccgt aatttccgcg
     2701 aggcaattca acagcagttg caaaattttc aagcagcctt gcgaaagttc ctgaggctgg
     2761 taaggatgtt cggtggcgat cccgtcgagg cgcgcgacaa attcatttac gcgcggatta
     2821 tatttcatgg tgcaagaacc gagcgggtac atgccgaggt cgattgcgta gttccaggtg
     2881 gaaagccgcg tgaagtggcg aaccacttcg atttcgctga cttcgggaaa gccgtcgatg
     2941 ctttcgcgaa cgaagtccga gcccagcgcg gtgcgtgggt ccacggccgg cacgtcgagc
     3001 ggaggtagct caaacgcgcg cttgccgggc gcggactcct cgaagatcag gccttcgttg
     3061 cggctgatgt ggcgtgcgac ggttttgata cgatcggtta gttttatttc ttcggttttc
     3121 atatttgttt tgtgtagcgc ccgccttcag gtgggcgcta caaatcaaat cggccgatca
     3181 aatcggcttg gacaaaatat cgcgaatggc atcggccatg cgctcgattt cggtgcgtga
     3241 aatggtttcc gtgacgcaga aaaggccgcg cttggtcaaa tcggaaaaat agctgcccac
     3301 gggcagcggg ccgataattt tgtgctttag caattcggca ttcaccattt ttacggagcg
     3361 cggaaactca accacgaatt cgttgaaaaa gggcccggta aaagcccggc gcacgcctcg
     3421 aattttttcc agttcatcga gagcgaagcg cgccttagcc aggttttgag ccgcaagttc
     3481 tttcaggcct tccttgccca gcaggcagag gtgaatgttt actgccagcg cgcacagcgc
     3541 ctggttcgtg cagatatttg aagtggcctt ctcgcggcga atgtgttgtt cgcgcgtggc
     3601 gagcgtgagc acgtaaccgc gttttccgct gctgtccacc gtttcgcctg caagtcgacc
     3661 cggcatctgg cggacaaatt tgtcgcgcgt cgcgatcacg cccgcataag gcccaccata
     3721 gctgggagcg aggccgaagc tttgcccttc catggcgacg atgtcagctt cagcaggcgg
     3781 cctcactgct cccaacgaca cgccttcggc gatggatacg accaggagcg cgccctttga
     3841 gtgcgcgaga gccgcgagac tgggcatctg ctccagaacg cccaaaaaat tgggagactg
     3901 aacgaccaca gcggcggttt cgctcgtaag cgcgttttca aggcgagtcg catccacctg
     3961 gccggaactg gtaaatccga tttcgttgat gcgcaatccg agattgcgcg tatacgtttc
     4021 gagcacctgc cgatattccg gatgcagcga gcgtgccacg atcacgcgat gccgaccggt
     4081 gaggcgctcg gccatcagca cagattcggt cgttgctgtc gaaccatccc acatcgaagc
     4141 attcgcgact tccatcccgg taagctggca catcaacgtt tgaaattcaa aaatcgcctg
     4201 caacgtgcct tggctgattt ccgcttgata gggcgtgtag gatgtgagga attcgcctcg
     4261 ctggatcagc gcatcggtga cgaccgaacg caaatgattg tagactccgg cgccgagaaa
     4321 actggtgtag ccaacggaat tttcttttgc gcgcgcgcga aaatattcaa tgatttcctg
     4381 ctcggacatc gcggcaggca gattcaacgg ttcttttaat cggaattgct cggggatgga
     4441 ttcgaaaagc gtttcgatcg atggcacctc aacggcatcc aacatttcgc ggcgctcgga
     4501 tgtactcttc ggaagatagc gcatcaggcc gaggcttcct tttcagagat gaacgcctgg
     4561 tatgcggcag cgtccatcag gccggagact tcggagggat ttgcgagctt gatcttgatg
     4621 agccatgcgc tgccatgcgg atctgagttc agggtttcgg gcgtgcctgt gagggtagcg
     4681 ttcgtttccg tcacttcgcc ggatacggga gcgaaaattt cgctgaccgc cttcaccgat
     4741 tccaccgtgc cgaatacttt tccggccgaa acttttgagc cgacagccgg catttcgacg
     4801 aatacgacat cgccgagttc atgctgagcg taatccgtga tgccgatcgt gcctgtgtca
     4861 ccagaaactt tgatccactc gtggtccttg gtgtagcggt agtcgtttgg atacatgacg
     4921 gctcctcggt aggacaggct tcagcctgtc tggcacatct ttaatatggc gcccgcaaaa
     4981 ttctcccgat actgtagcgc ccgcctttag gcgggcaagc ggttgactgt acagtgcccc
     5041 ctgaaggcgg gcactacaga atcgcgggaa cgattctact tcgttctctt ataaaacggc
     5101 atcggaacaa tttttgcggg ggcctgattt gcgcggatcc cgattgagat ttccgttccc
     5161 ggagttttgg atgccgtcgg cacatacgcc atgccaatgt tctttttcaa aaagggcgca
     5221 ggcgaaccgc tcgtcacgcg gccaaccggt tcgttgccga aggaaacaac gcagccgtcg
     5281 cgcgcaatga gccgatcctg catttcgatt ccgaccagag tgcgatcgac tcctttttcc
     5341 ttctgtttca caaggatatc gcgtcccagg aattcgcttt tgttgggttt gcaaatccag
     5401 ccgagcccgg cttcccatgg cgtggtcgta tcgtcaattt cgtggccgta caggcacata
     5461 gatgcttcca gtcgaagtgt gttgcgcgca cccaggccac agggcagcaa gccatggccg
     5521 gaaccggcgt ccagaagcgt ggcccatagt ttctcggaat gttccggcgc gaagtaaatc
     5581 tcgaagccgt cttctccggt atatccggtg cgtgcgatta tgcagtcgac gccatccgct
     5641 ttgccgaaag caaaatggta atacttgatc ggctcaagcg gcacttgtgt gaagcgttgc
     5701 aggatgccga gagccttcgg tccctggatg gccagttgag cgtagcgttc gcctgcattt
     5761 tcgatttgcg cgccggcgcc gttgtgcgcg cgaatatgct caaaatcctg gtcctggttg
     5821 ctagcgttca cgcaaaggaa gtagtgcgta tcggaaatct tgtgcacgag taagtcatcg
     5881 acaaacgtgc cctgcgcggt catcaatccg gaatattgcg cttgcccaat gacaagtttc
     5941 gaggcgtcgt tacacgtaac atgctgaacg agttcgaggg cgtgcggtcc gcggacctcg
     6001 atttcgccca tgtggctgac gtcaaacagg ccggcagccg tgcgcgtagc catgtgctcg
     6061 gcaacgatgc ccgagtactc gacaggcatg tcccagccgc cgaaattgac cattttggcc
     6121 ttgaggcgcc ggtgcacggc gttcaatgct gttttgcgca gatgtgtagt cgatggagat
     6181 gtcactcgaa attctcaggg acagcagagc aaaatcgaaa ctaacacgcg ggtttgaaag
     6241 gtgtcaagaa agcatggcca tcgtcgtccc aaaccgcgcg aagagtaggg caagagggaa
     6301 cagaagcctt ggaatttgta gaggccggcc gaattgtgag cgggcttccg gtcgcatggg
     6361 tctggttaaa cgatgcccca tgataagaaa gcgtgccctc ttcttgttga aatgcgagag
     6421 aagaagaatt ccctgcgaaa gacaagtgcg aaaaggctag attgaggcgg gttcgcgcat
     6481 atatcggcct tgttcttgcg ttgtggggta tcctatagtg ggttccaggc ggcttgcgcg
     6541 agggtttttc gctgttttcc gttgtacgaa ggaaaaatat ggaaagcact gttctttgtt
     6601 cgcaatgcga tctgccggaa ggcaaatgcc tgtgcgaccg gtactgctgc tattgtcagg
     6661 ggcagtatgg agtccgattg tgtatggatg gcaaatacta ctgtccggat tgccgggaag
     6721 cctgcgaagt cagtgtggtt caatccgatg accgatgagc ctatacgcac cgttttccac
     6781 ctcgataagg attcgcgcct ggtcagtgtg ttttgctccg ctgttgagca tcaagcggcg
     6841 catgcggggc ttgagggtgg ggcgggagtg caactagcac aggctgcagg ggatgtctgc
     6901 cgcgaggcaa tttcgcaggg aggcggggat acaggcattg aagtgaccct ggatactttt
     6961 caggaccgcg tggaggtcgc tatccattac agggggcagg gattgcccac ggatgggacc
     7021 ggaagccctg tggtcactgg gacgaagtcc aagggcgcgg aaagcttgaa cggacaggaa
     7081 ttgctttcgc gcgttgaccg cgtcaattgt aaggcggatg aggggattgc acgagtcacg
     7141 ctagtaaaat atttgcctgc acatcactga tattcatctc ttcttagtcc accgttaaga
     7201 aatcgttttg gatatgagtt taggttgcga gggttgcgag ggggcgtagc atggccagga
     7261 tgacgcagaa aaaatcgcgc aaaaaa
//
LOCUS       DASZMB010000039         7332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3602780, whole genome shotgun sequence.
ACCESSION   DASZMB010000039 DASZMB010000000
VERSION     DASZMB010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7332
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3602780"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1475)
                     /locus_tag="VG272_01755"
     CDS             complement(<1..1475)
                     /locus_tag="VG272_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWF12432.1"
                     /translation="MAKITGGRFIAEFLNAQGVTAFFFVPTMLSRALAEMDNMPIKRV
                     LTHGEKAAAYMADGYARASGRPGICAAQAVGAANLAAGLRDAYMGHSPVIALTGGRWG
                     HQKHKINYQEMDDFPMLTQVTKANFQADTVTRIPDLLRQAFREATSGTPAPVNVLFAG
                     KEGDIEHDEAELKMIPEEGFTHVPAFRPAPEAARVAAAARILKEAQKPVIVVGGGARL
                     SGAGAEVLKLAEALSIPMATSLNGFSLVPDNHPLYIGVPGTYSLSCTNKILSRADLVL
                     YIGSQTAGQVTHFWKIPAVGTPVIQIGIDPSDLGRNYPNRVSILGDAKTALQMLNQVV
                     AADENKGSRNTKWLEETSAAVNAWRAEVKPLRESSAEPMRPERLMKELGDWLPQDTIV
                     VCDTGHAGMWCAQQLWVTKPWDFLRAAGSLGWAFPASLGAKVAMPKKPVVCFTGDGGF
                     WYHLQELETAVRCGIPTVTVVNNNNALNQETLIFKQAYGGNP"
     gene            complement(1540..1614)
                     /locus_tag="VG272_01760"
     tRNA            complement(1540..1614)
                     /locus_tag="VG272_01760"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1580..1582),aa:Met,seq:cat)
     gene            complement(2098..2277)
                     /locus_tag="VG272_01765"
     CDS             complement(2098..2277)
                     /locus_tag="VG272_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12433.1"
                     /translation="MAETAAPANGHRMAEMAAPASDHQMVETVALGSDHQMAETAVLA
                     LGSDRQMAEMACLAW"
     gene            2341..3036
                     /locus_tag="VG272_01770"
     CDS             2341..3036
                     /locus_tag="VG272_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12434.1"
                     /translation="MLDPVNYAIWLAVLLVEIFSFVCLLRKKAYSKHFTLVFYLCACV
                     ATDIGSYSIIHTSGYESDAYFYFYFYSQSLLTICLYFVLMNLYSHVFSEMGVGQYIRA
                     CALLILGGTAGISYYLVAVSSNRMVTHFVAELGQNLYFVGVILTYLLWGAMAKPRENR
                     TRLLQLVLSMGVYVSLSAGSYAMNNLYPNHTFWQYYFPVTSMWLPLSWAYTFTKVPAD
                     AQLATARVLAPNQ"
     gene            3066..3437
                     /locus_tag="VG272_01775"
     CDS             3066..3437
                     /locus_tag="VG272_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12435.1"
                     /translation="MTLLQFFVSYSRSIIAESRGHRLSEQACEICGIKSRSLAGEQFG
                     RLNQLIALCPEVGDDGIKVKAVAVYFHLLGFVKTVVSWAMPSASPWIEAERGGCAYAV
                     AVVLDRRIAHNRMVMADMASH"
     gene            3604..5094
                     /gene="tilS"
                     /locus_tag="VG272_01780"
     CDS             3604..5094
                     /gene="tilS"
                     /locus_tag="VG272_01780"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HWF12436.1"
                     /translation="MNRDLHIRVCHTIASHSMIRPGDRIGLGVSGGADSVAMLRIFAD
                     LQTKLGITIFVLHFHHQLRGADADEDERFAKILADQFHFPFVSGRSDVASEAKLQGRN
                     VEDAARRLRYEFFNSAAEAHGLNRIAVAHTADDQAETVLSHMLRGTGLTGLAGIYPVA
                     GLIIRPLLESNRQQLREFLSGLGQPWREDITNQDTSHMRARIRHHLIPLLLRDFDSAA
                     VTRLARLATHAREDEAFWRSVEDARFEALATRESSSTISVKISDLLFPLQSSAFPISQ
                     DQQTTNSTMALTRRLIRKIYAELRGGREQLTSRHVDSMVDLVTKSQSGARINLPGIWV
                     ERIFDRLVFTNDIASAEAMQQGESPGVNRGFAYTVSRPERLDSASIVVTEIHRRFNLK
                     IVDWPLCPRDTVSGIIALDFERVQWPLVLRNWLPGDSYRPQGSGSARKVKRLLLESRI
                     PRSSRASWPILTSQGKVIWASGYPVAEEVAAHSRTKSGLVIAEEEL"
     gene            5338..7269
                     /gene="ftsH"
                     /locus_tag="VG272_01785"
     CDS             5338..7269
                     /gene="ftsH"
                     /locus_tag="VG272_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HWF12437.1"
                     /translation="MNPSSTLKTVMFWLFIIMLAVLLWTLVSKSGQSAREEEPSYSEF
                     MAQVDRGNVKEVTLYLSPTSYEIEGEWREPSKKFRTTIFKESAPDLTKTLRDKQVLIN
                     VKEVNRADWVTFLLNAAPLFLLVAFWIFMMRQMQAGGNKALSFGKSKARLLTAQQKKA
                     TFKDVAGAEEAKEELQEIIEFLKDPQKFQKLGGRIPKGVLLVGPPGTGKTLLARAIAG
                     EANVPFFSISGSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFIDEIDAVGRHRGAGLG
                     GGHDEREQTLNALLVEMDGFESNEGVILIAATNRPDVLDPALLRPGRFDRRVVVARPD
                     VKGREEILRVHTRKVPLNEDVDLSVIARGTPGFSGADLANLVNEAALWAARQGRKTVF
                     MIDFEMSKDKVLMGVERRSMILSDEEKKNTAYHEAGHALVAAITPGTDPLHKVTIIPR
                     GMALGLTMQLPIDDKHTYTKTYLEGTLTVLMGGRCAEEIFLGHITTGAGNDIERATDI
                     ARKMVCEWGMSTLGPLAYGKKEEAIFLGREIAQHRDFSEDTAIRIDQEVHGIVTTAYN
                     NARGILENNRDVLERIALALLDREVLDATELRLLMENKPLPEKIRPTPPSPPQPLPGA
                     EPKPAPRPELRPAPGFSKG"
BASE COUNT         1593 a         2005 c         1987 g         1747 t
ORIGIN      
        1 gggtttccgc cgtaggcttg cttgaagatc aacgtttcct ggttcagcgc gttgttgtta
       61 ttgacgactg taaccgtcgg gatgccgcag cgtactgcgg tttcgagttc ctgcaggtga
      121 taccagaagc cgccgtctcc cgtgaagcag accacaggct ttttgggcat ggcaactttc
      181 gcgccaagcg atgcggggaa agcccagccc agggatcctg ccgcgcgaag aaagtcccac
      241 ggcttcgtaa cccagagctg ctgcgcgcac cacatgcctg cgtggcccgt gtcgcacacg
      301 acgatcgtgt cctgcggcag ccagtctccc aattccttca tcaggcgctc ggggcgcatc
      361 ggctcggcgc tggattcgcg caacggcttc acttcggcgc gccacgcatt caccgcagcg
      421 ctcgtttctt cgagccactt ggtattgcgg ctgcccttat tttcgtccgc ggcaaccacc
      481 tgattcaaca tctgcagagc agttttcgcg tctcccaaaa tcgatacacg attcggataa
      541 ttccggccca gatcgctcgg atcgattccg atctgaatga caggcgtgcc cactgcggga
      601 attttccaga aatgcgttac ttgtcctgcg gtctggctgc cgatgtacag cacgagatct
      661 gcgcgtgaca agattttatt tgtgcacgaa agcgaatacg ttccagggac accgatgtag
      721 agcggatgat tatcgggtac gagcgaaaag ccgttcagcg aagtcgccat cggaatggaa
      781 agcgcttccg cgagcttcaa cacttcagct ccggcaccgg acaaacgcgc gcctccgcca
      841 accacgatca ccggcttttg cgcttctttc aaaatccgcg cagccgcggc aactcgcgca
      901 gcttccggag cgggccggaa tgcgggaaca tgcgtgaaac cttcttctgg aatcattttt
      961 aattcggctt cgtcgtgctc aatgtcgcct tcttttccgg cgaacaaaac gtttacggga
     1021 gcgggtgtac cggacgtggc ttcgcgaaac gcctgccgca acagatcggg aatgcgcgtc
     1081 acagtgtcag cctgaaagtt cgctttggtc acctgcgtca acatcgggaa atcgtccatt
     1141 tcctgataat taattttgtg cttctggtgg ccccaccggc cgcctgtcag cgcaatcacc
     1201 ggactgtggc ccatgtacgc atcgcgcaaa cccgcggcca gattcgccgc gcccacggcc
     1261 tgcgccgcgc aaatgccagg acgtccggaa gcgcgcgcat aaccatccgc catgtaggcc
     1321 gcagctttct caccatgcgt taaaacgcgc ttgatgggca tgttgtccat ttccgctaac
     1381 gcgcggctca gcattgtggg cacgaagaaa aaagccgtaa caccctgcgc atttaaaaat
     1441 tccgcgataa atcggccgcc agtaattttt gccacagtct gctcccttga tatgaggaat
     1501 aatttttcaa aatgaattca aatcaattat ttaatgaaat ggtgccggcg gagagactcg
     1561 aactctcgac ctagggatta tgagaccctc gctctacccc tgagctacgc cggcaaaata
     1621 aagcgcttca tgttacggat ggattccgag ggctgtcaag caaggaaagg ttacctgaac
     1681 ttgtgccgct tgtccaagag cgccgcacac tggcccgatt cggaacgaaa tatcgatgtt
     1741 attgagaaga taggccctta gtcccaggtt ctagtagtac tgatgtactg ttgaccccga
     1801 cggagtgaaa ttctagattg tacgtggaca gtgcgattgg aactgctccg gtaacggacg
     1861 gcatgagccg accgccggga cattgtccaa gggtattgtt cgtttagaca cacggtaaca
     1921 tttatgttcc attaaggaac gggtgtacat atactccgtc cgggttagtt ttccgtccgg
     1981 aggggtctat gtacaagtct ctagcggtta ttgcttttgc gcttctcgtt ttgtcgggcg
     2041 cgatggggct gaggaatgtg gctgtggcaa acgcctcaac ttccgcaagc tccagcttca
     2101 ccatgcaagg caggccattt ccgccatctg gcggtcactt ccaagtgcaa ggacggccgt
     2161 ttccgccatc tggtggtcac ttccaagggc gaccgtttcc accatctggt ggtcacttgc
     2221 aggggcggcc atttccgcca tccggtggcc atttgcaggg gcggccgttt ccgccatctg
     2281 gtggtcacta gaaatagcgt gaaccttagt tagctgggtc cacgcggagg aggtcagacc
     2341 gtgctggacc cagtcaacta tgcgatctgg ctagccgttc tcctcgttga aattttctca
     2401 tttgtttgcc tgcttcgaaa gaaagcctac tccaaacatt tcactctggt cttttatttg
     2461 tgtgcatgcg tagccacgga tattggcagc tatagcatta ttcatacatc gggctacgag
     2521 tcggatgcct atttttactt ttatttctat tcccaatccc tgcttaccat ttgcctctat
     2581 tttgtgctca tgaatttgta ctcccacgtc ttttcggaaa tgggtgttgg gcaatatatt
     2641 cgcgcttgcg cattgttaat tttaggggga acagctggaa tttcctatta cctggtcgcc
     2701 gtttcgtcca accgcatggt tacgcacttt gtggctgaac tcgggcaaaa cctgtatttt
     2761 gtcggcgtga tcctgaccta tctgctttgg ggcgccatgg caaagccgag ggaaaacagg
     2821 acgcgcctgc tgcagcttgt cctttccatg ggcgtatacg tgagtttgtc cgccggaagc
     2881 tatgcgatga acaatctcta tccaaatcat actttctggc agtactactt cccggtaact
     2941 tccatgtggt tgccgctatc atgggcttac acgtttacaa aggttccggc agacgcgcag
     3001 ttggccacag cgagggtcct ggcccccaat caatgatcgc gtgcttaata ttcgtggtct
     3061 cttccttgac gctgttgcag ttctttgtct cctacagccg ttctattatt gcggaatcgc
     3121 gcggccacag actctcggaa caggcttgtg aaatttgcgg aattaaatcc agatcactgg
     3181 ccggtgagca gttcgggcgc ctgaatcagt tgattgcgct ttgcccggaa gtgggcgacg
     3241 acggaattaa ggtaaaagcg gtcgccgtct atttccattt attgggtttt gtgaaaaccg
     3301 ttgtgagttg ggcaatgccc tccgccagcc cctggattga agcagagcgc ggcggctgtg
     3361 cctatgccgt ggctgttgtg ctggatcgcc gaattgctca taaccggatg gttatggcgg
     3421 acatggccag ccattaactt cgagcaactt gatttacttt ccgcacgccc ttttggaatc
     3481 gctcttccgg ctgtgagatt ctccccagac ttgttaccat tcttgtttcc ggatttccgg
     3541 ctgtgtccaa cacaattcct catgcgacaa ttaaatcgac cgccgcgacg cgcataaatt
     3601 tagatgaatc gagatcttca tattcgcgtt tgtcacacaa ttgccagcca ctccatgatc
     3661 cgtccgggcg accggatcgg actcggagtt tcgggcggcg cggattccgt ggccatgctg
     3721 cgtattttcg ccgacttgca aacaaaactc gggattacga tttttgttct acatttccat
     3781 catcaattgc gtggggcgga tgcggacgag gatgaacgct tcgccaaaat tcttgcggat
     3841 caatttcatt ttccgtttgt atccggtcgc tccgacgttg cgagcgaagc caaactccag
     3901 ggaagaaatg tggaagatgc ggcgcgacgg ttgcgctatg agttttttaa ttccgcggcg
     3961 gaagcgcatg ggctgaatcg tatcgctgtc gcccataccg cagatgacca agccgaaacc
     4021 gtgctttcgc acatgcttcg tggcactgga ttaaccggct tggcaggcat ttatcccgtc
     4081 gctggactca tcattcgccc actgcttgaa tcgaacagac agcagttgcg cgaatttctt
     4141 tccggtttag gacagccctg gcgggaggat attaccaatc aagatacttc tcacatgcgt
     4201 gcgcggattc gtcaccacct gattccattg cttcttcgcg atttcgattc cgccgccgtg
     4261 acgcgcctcg cacgtctggc gacgcatgca cgggaagatg aagctttttg gcgatcggtg
     4321 gaggacgcgc gttttgaagc tttggcaact cgagaatctt catcgacaat ttctgtaaaa
     4381 atttctgacc tcctatttcc tttgcagagt tccgcattcc ccatttcgca ggaccagcaa
     4441 accaccaatt ctacaatggc attgacccgt cgcctgattc gtaagattta tgctgaactt
     4501 cgcggcggcc gtgaacaatt gacttcgcgc catgtggaca gcatggtcga tctcgtcacc
     4561 aaatcgcaga gcggggcgcg catcaacctg ccgggaattt gggtggaacg aatttttgac
     4621 cggcttgttt ttacaaacga tattgcctcc gccgaagcaa tgcagcaggg cgaatcgccc
     4681 ggagtgaatc gcgggtttgc ctatacggtt tcgaggccag aaaggcttga ttctgcttcc
     4741 attgttgtta cggaaattca ccggcggttc aatttgaaaa tagttgactg gcctctatgc
     4801 ccgcgcgaca ctgtatcagg tataattgct ctagactttg agcgcgttca atggccattg
     4861 gttcttcgga attggctacc gggcgactcc tatcgtccgc aggggagtgg tagcgctcgc
     4921 aaagtaaagc gattactcct ggaatcccga attccccgaa gctcacgggc aagctggcca
     4981 attttgacca gccagggaaa agtgatttgg gcgtcaggtt acccagtggc cgaagaagtt
     5041 gcggcgcact cgaggacgaa aagcgggtta gtcatcgccg aagaagagct ttaaaatccg
     5101 gcggctagca atgaggttgg gagagccgac cggttggatt gtagtaatta ccgtacattt
     5161 tagccaaaag cccttgatga ctttggctat tggcctgcat ctaatcactt aggccagtcc
     5221 gaatttttca ggaatgggcc ccaaacagtt gaagcaacag tagtaatatt aatagttatt
     5281 ttgcgctggt gaatttacga ggcagcgcgc cggaagatcc ggcaggcgag gtaagtcgtg
     5341 aaccctagtt cgaccctgaa aaccgtaatg ttttggctgt ttattattat gctggcggtg
     5401 ctgctgtgga cgctggtctc caagagcgga caatcggcgc gtgaggaaga acccagctac
     5461 agcgaattca tggcccaggt ggaccgcggt aacgtaaagg aagtgacgct gtacctctcg
     5521 ccgaccagct atgagatcga aggcgaatgg cgcgaacctt ccaagaagtt ccgcaccacc
     5581 attttcaagg aatccgctcc ggacctgacc aagacgctgc gcgacaagca ggtcttgatc
     5641 aatgtgaagg aagtaaatcg cgcggattgg gttacgttcc tgctgaacgc tgctcccctg
     5701 ttcctgctcg tagcgttctg gatcttcatg atgcggcaaa tgcaggctgg cggaaacaaa
     5761 gccttgagct tcggtaaatc caaggcgcgc ctcctgaccg cacagcagaa aaaagcgacc
     5821 ttcaaggatg tggctggtgc agaggaagcc aaggaagagc ttcaggagat cattgaattc
     5881 ctgaaagatc cgcaaaaatt ccagaagctc ggtggacgca ttcccaaggg tgtgttgctc
     5941 gtcggtcctc caggaaccgg aaaaaccctg ctggcgcgag cgattgccgg cgaagcgaat
     6001 gttccgttct tttcaatttc gggatccgat ttcgtcgaaa tgtttgtcgg tgtcggcgca
     6061 agccgcgtgc gcgatctgtt tgagcagggc aagaagaatg cgccctgcat tattttcatc
     6121 gacgaaattg acgcagtggg acgccaccgt ggtgcgggcc tcggcggcgg acacgatgaa
     6181 cgcgaacaaa cgctgaacgc cttgcttgtc gaaatggacg gatttgagtc caacgaaggc
     6241 gtcatcctga tcgccgctac gaaccgcccg gacgtgctcg atcccgccct tctgcgcccc
     6301 ggccgatttg accgtcgcgt agttgtcgcc cgtcctgacg tcaagggccg cgaggaaatt
     6361 cttcgcgtac atacgcgcaa agtgcctctg aacgaggacg tcgatctgtc cgtgattgcg
     6421 cgcggtacgc ccgggttctc gggcgcggat ctcgccaatc tcgtgaacga agctgcctta
     6481 tgggccgcgc gtcagggacg caaaactgtt ttcatgatcg atttcgaaat gtccaaggac
     6541 aaagtcctga tgggcgtcga gcgccgctcg atgattctct ccgacgagga aaagaaaaac
     6601 accgcgtatc atgaagcggg gcatgcgctc gtcgcggcaa ttactccggg caccgatcct
     6661 ctgcacaagg taacgattat tccgcgcggc atggcgcttg gcttgaccat gcagcttccg
     6721 attgacgaca agcacactta caccaagacg tatctcgagg gaacattgac cgtgctgatg
     6781 ggcggccgtt gcgccgagga aattttcctt ggccacatca ccaccggcgc ggggaatgac
     6841 atcgaacgcg ccacggacat cgcacgcaag atggtttgcg agtggggcat gagcactctc
     6901 gggccgctgg catacgggaa aaaggaagaa gcaattttcc ttggccgcga aattgcccag
     6961 caccgcgact tctcggaaga cacggccatt cgtattgacc aggaagttca cggcatagtg
     7021 accaccgcgt ataacaatgc acgcggcatt ctcgaaaata accgcgacgt tctggaacgg
     7081 atcgctctcg ctctgctcga ccgcgaagtt ctcgacgcaa ccgagcttag gctgctcatg
     7141 gagaacaagc cgctgccgga gaagattcgt cctacgccac cttcgccgcc acaaccgctg
     7201 cccggcgccg agccgaaacc cgcgccacgc ccggagctgc gtcctgctcc aggcttcagc
     7261 aagggctagg ttattcgaat ctccaaagtc agtcccgtag gggacgatac accgtagccc
     7321 accaccgcag tg
//
LOCUS       DASZMB010000040        19061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3883723, whole genome shotgun sequence.
ACCESSION   DASZMB010000040 DASZMB010000000
VERSION     DASZMB010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 19061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19061
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3883723"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VG272_01790"
     CDS             <1..370
                     /locus_tag="VG272_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWF12438.1"
                     /translation="ENFLVFSGDDAITLPLVAMGGRGIISVASNVIPAEMTNFAQLCL
                     AGNFAEARTWLRKWMPLLEVNFVETNPTPVKAAMAEMGLLEPVFRLPLVPPRVESATK
                     IRSVLESLALLERAHVANRS"
     gene            330..1211
                     /locus_tag="VG272_01795"
     CDS             330..1211
                     /locus_tag="VG272_01795"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: protein motif:HMM:NF008808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HWF12439.1"
                     /translation="MLYSKGLTLPTAPEKLEKHIEALFDSKPARYSAIDHQFFRNFKN
                     ELNAGRIRSAEPDSSRVTGWRTNEWVKKGILVGFRMGVVVDMSLDSVKQPFFDKDSYP
                     VRQFNEGDAVRIVPGGSSIRDGCYIGRDVVCMPPMFVNAGAYVDDGTMIDSHALVGSC
                     AQIGKNCHISAASQIGGVLEPVGAMPVIIEDNVLVGGNCGVYEGTIVKSRAVLGTGTI
                     LNRSTPVYDIVRGTVYMATELNPLVIPENAVVIAGSRAISHGVGKEWGISLYTPVIVK
                     YRDAKTDAKIQLEDLLR"
     gene            1636..2076
                     /locus_tag="VG272_01800"
     CDS             1636..2076
                     /locus_tag="VG272_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12440.1"
                     /translation="MKNVFVGNMNFQTTESELRALFEPFGQVTRVHIAMDRETGRARG
                     FAFVEMPNDAEATKAMSGLDGREVGGRALKVNEARPKAAGGGGGPRGGSGGSGGGGFD
                     RGGDRDRDRDRNRGGSGGRGGGKGGRFSTEDYRDSPRQPREPRW"
     gene            2144..2338
                     /locus_tag="VG272_01805"
     CDS             2144..2338
                     /locus_tag="VG272_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12441.1"
                     /translation="MATSKSTFQKRQKEMKRQEKQRAKAERRAQKKLLGPQDVDFPME
                     PLTGPIIDPDISFPEPDEES"
     gene            complement(2504..3643)
                     /locus_tag="VG272_01810"
     CDS             complement(2504..3643)
                     /locus_tag="VG272_01810"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HWF12442.1"
                     /translation="MKILVAIAGISILLITLWEAFETIILPRRVTRPVRIVRIFYRTT
                     WVFWAAVNRLFRTKKMREAHLSYYGPLSLLGLFAAWALLLISGFAMLHWAAGSAINTT
                     DGSPTFRTDFYLSGTTLFTLGLGDVTPRTALARVITVCEGGTGFGFLGLMISYLPTLY
                     GAFSQRELNISLLDARAGSPPTAGELLRRHAQFAHSEALTPYLRDWELWVAQLMESHL
                     SYPVLCYFRSQHDNQSWLAAMTTVLDVTALMIAYGQGMAKWQAQLTFAMSRHCLVDLA
                     EVLRVRASPPGRDRLPPSDLPRVRELLLECKASTNCNEAGDKKLRELREMYEPYLNGL
                     SKRLLIDIPSWGVGRHFVENWKRTAWDKISGPASSGSTNVESSHF"
     gene            complement(3672..4979)
                     /gene="thrC"
                     /locus_tag="VG272_01815"
     CDS             complement(3672..4979)
                     /gene="thrC"
                     /locus_tag="VG272_01815"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWF12443.1"
                     /translation="MKYRSTSHRSPEASLRDAVLCGSAPDGGLYMPVEMPLLEKDFLD
                     RLPSLTLPEIAMEVGSLFTGDEIPHPVLQKIVTEAFNFPVPLVTLSERLHILELFHGP
                     TLAFKDFGARFMARLMGYFVQESGKELTVIVATSGDTGSAAAHAFLGLAGIRVIILYP
                     SGRVSEAQEKQLTTLGQNITALEVAGSFDDCQRLAKQALVDPVIAEKLTITSANSINI
                     ARLIPQSFYYFSALAQLKTAGTLPAFAVPSGNFGNLTGGLLAKRIGLRTAQFIASTNA
                     NDVVPEFLRSGNLIPRASRHTISNAMDVGNPSNFARIVDLYGNDLQAIRRDIWGCSFS
                     DEETLRMMHDVNERHGYVLDPHTAVGVLGWQSFAKQNPPTTQGIVLATAHPAKFDETV
                     ARATGVRPEMPERLAAILNRQKKSIPFPNRFAELQEFLLTTNS"
     gene            complement(4976..5617)
                     /locus_tag="VG272_01820"
     CDS             complement(4976..5617)
                     /locus_tag="VG272_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12444.1"
                     /translation="MFKISLIGMSGAGKSYWSQKFAGAGFHVIGVDDRIEEKLAPELA
                     KDGYRGIGGVAAWMGWPDSPTYRAREKKYLDYEVEAMREALNEIEASGNQGIILDTTG
                     SVVYTGDEICRRFQKLTTVVYLEASPDEENLLIARYMSSPKPVLWEDQFVPRAGESTP
                     DTIERCYPQLIAHRKEMYKRYAHRIVPMDLMWDLTIDARGFLEYLEAQARPAQ"
     gene            complement(5784..6311)
                     /locus_tag="VG272_01825"
     CDS             complement(5784..6311)
                     /locus_tag="VG272_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HWF12445.1"
                     /translation="MIRALHKATTTFESPVNAIWRPWFGRNLIGNGQLVFGHCDVGPW
                     NVVARAGKPIALIDWEVAGPVDQLVEFAQACWLNVQLHDDDIAARQGLLPVEDRARQL
                     RVMSDGYALSTSDRLKLVDTMISFAIQDAADQAIQVPVTPESQDATPLWGLAWRTRAA
                     AWMLRNRTLFDRALL"
     gene            complement(6645..7955)
                     /gene="bioA"
                     /locus_tag="VG272_01830"
     CDS             complement(6645..7955)
                     /gene="bioA"
                     /locus_tag="VG272_01830"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: protein motif:HMM:NF004624.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HWF12446.1"
                     /translation="MSASDLHLWHPFTQEGIDPAPLVVRGGKGVFIEMQDGRQILDAI
                     SSWWVNLHGHSHPAIAQAVAEQAAKLEHVLFAGFSHEPAEELAFRLGKVIPAPLRHVF
                     FSDDGSTAVEVALKLALQYWKNCGRLKKSRIVALENAYHGDTVGAMSVSDDSPFTAAF
                     DSLRIPVLRTHSASCAHCPVGLTRSTCHIECLNKLESLLNEQGEEIAVVIVEPLLQGA
                     GGMIVHPEEFLAGVRRLTEAHNVLLIADEVLTGFGRTGRMFACERAAVVPDLMCVAKG
                     LTGGFLPLAATFATDRIHDAFVGGDRMRSFFHGHSYTANPIACAAANASLQIFEHEPV
                     FERIAAIEKGHTERLPAFAAHPGVADVRHIGTVAAIELKVPDAGYLSSLRPRLYQYFL
                     SRGVLLRPLGNVVYILPPYVITPEQLNFVYDVIRDSLALISSTK"
     gene            complement(7959..8588)
                     /gene="bioD"
                     /locus_tag="VG272_01835"
     CDS             complement(7959..8588)
                     /gene="bioD"
                     /locus_tag="VG272_01835"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HWF12447.1"
                     /translation="MSGYFVTGTDTNVGKTVLSALLVAALDAHYWKPVQTGAVEGTDR
                     ESVRKWTGISEDLLPSEKYRFDPPVSPHLAARAAGIELHLDDFELPAAPADAKWIVEG
                     AGGVMVPINTCELMRDLMRRIGFPVVIAARTALGTINHTLLTFAALRELSLPICGVVM
                     IGDENVENRRAIEHYGNVRVVGHIPLLETIHRATLLEVYEKYFDHQAFQ"
     gene            complement(8585..9748)
                     /locus_tag="VG272_01840"
     CDS             complement(8585..9748)
                     /locus_tag="VG272_01840"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HWF12448.1"
                     /translation="MDIQKRILAELSELESRAELRQLQMVHGIDFSSNDYLGLATDPR
                     MKQAIAEGVEYAARIASTGSRLLSGQSDTWTMLEQDFARWVGTDAALYFTSGYAANLG
                     LLSAILRPEDIVFSDSANHVSIIDGIRLTKSKRVIFPHLNLEFLENEFRRNASASGAR
                     IIVVESIFSMEGDRAPLTELAALADRYGAELIVDEAHAIGVRGPNGSGCVAEAGLSTR
                     VLATVHTCGKALASAGAFVCGSENLQKLLINRARTFIFNTALPPYFASQVSAGVKLAA
                     DADAGRARLAQLSAFLRNELRTNGFYIPEIDSQIVPIILGWNDAAVQFAEYLRARGFG
                     VRAIRPPTVPPGKARLRISLTAKHSEDILAELVLAMVQARNEYSTARAVSVSQ"
     gene            complement(9758..10747)
                     /gene="bioB"
                     /locus_tag="VG272_01845"
     CDS             complement(9758..10747)
                     /gene="bioB"
                     /locus_tag="VG272_01845"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HWF12449.1"
                     /translation="MSHNDAGISAARAALRHDWTREQIQQIYRQALPDLLFSAQQVHR
                     QFHDPTRVQFCQLLSVKTGGCPEDCSYCPQSAHYSTEVTRGGLLPVDEVLEFAKRARG
                     EGATRFCMGAAWRNVQEGDDFENILKMVRGVAEFGMEVCCSLGMLDDSQAKRLAEAGL
                     TAYNHNLDTSPEFYGQIITTRNYDDRLRTLASVRKAGVTVCCGGIIGLGESENDRIGL
                     LQQLSTLNPHPESVPINALVRVTGTPLEQVDSLDPLVFVRTIATARILMPASMVRLSA
                     GRKALSREAVALCFVAGANSLFIGEKLLTTANPEPQDDMAMLQSLGLEPLPLQ"
     gene            complement(11540..12727)
                     /locus_tag="VG272_01850"
     CDS             complement(11540..12727)
                     /locus_tag="VG272_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HWF12450.1"
                     /translation="MDSNSVLTENPAAAQTKTAEAQAKPAVPTAALVATLLQVSKQVS
                     DLIFSPGRAPQVELNGQLKEINITGVGRLTPEVTHRIALDLIGQNEIVTSKLEKEGAT
                     DLSYSLPGLARFRVNVFRQRSTYAIVMRVIPQTVPDFASLNLPAALGEIVGLRNGIVL
                     VTGPTGSGKSSTLAAIINKINEEKACHILTIEDPIEFLHLHKKSTIHQRELHSDTPSF
                     ALALRAALRQAPKVILVGEMRDRETIEIAMEAAETGHLVFSTLHTVDASKTIERVVGV
                     FPLADQQTIRSRLSRSFRYIVSQRLIPKKDKSGRVAAIEILKSTMRTREYVEKGEMEG
                     KSLLDAMRDGATEGMQHFDGELERLVRSDVIDLESALSYASNSGNLRLELSDLAEAAP
                     ASV"
     gene            complement(13174..15054)
                     /locus_tag="VG272_01855"
     CDS             complement(13174..15054)
                     /locus_tag="VG272_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWF12451.1"
                     /translation="MKRKKLGEVLQARGQISAANLQRLFKEQESKMVRLGELILETGL
                     VDKTSLVKALEEVSRVPYVDSATIRSDQEALQLIPKSMAQRLDILPIQMDQTRLVVAM
                     AEPQNVTTIDEVRFTSGKDLSPRFGFRAEIQAAITRNYAQLEPVLNSRNASTAAGDNS
                     SEIEQDVEFISTSSRQANRDAIQEIQAELNKRKTPAVRLVSEIIQKAIAKHASDIHVE
                     PQAICTTIRIRVDGVLRELETVPLNIQNSLISRLKILADMDIGERRAPQDGRFMVAIG
                     KSRVDMRVSTLPTQYGEKVVIRLLEATAPASTLGELGLPPNLAARLLQSLAQPQGMLL
                     ITGPTGSGKSTTIYAALNLLRKPAVNVVTVEDPIEYALPGVNQVQVNTRAGLTFASCL
                     RSILRQDPNVIMIGEIRDLETAEIAMKAAQTGHMVLSTLHTNDSVSAVARLLDLGIPE
                     YLVASSVSSILAQRLVRKLCSCHGLKDVTPAYAERLMAVGWNNPPTQIAWPKGCKVCD
                     FTGYKGRIGIYELMMIDESIRAILRGTYKPDQVRDAARAAGMQRMQEDALEKLQAGVT
                     TLDEIIRVVPMDIQSDNVFEHCGKKLSALFRFCPYCGIDREAEGLHPTTSSLDTREGV
                     LT"
     gene            complement(16219..16464)
                     /locus_tag="VG272_01860"
     CDS             complement(16219..16464)
                     /locus_tag="VG272_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12452.1"
                     /translation="MTLMEITYELQSRLQPDQLRALGGFANTYGLRRFRVDEDANRLS
                     FEYDASRLKETEVEHVLRMAKIPVLRRVQPVGTISQV"
     gene            16563..16922
                     /locus_tag="VG272_01865"
     CDS             16563..16922
                     /locus_tag="VG272_01865"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HWF12453.1"
                     /translation="MAGKAATKLKIETSCGCGTNETSRKALAVDPEIKSANLKRLRRI
                     EGQVRGLQRLVGEDRYCADILIQISSVQEALRSVGRALMKNHLRHCASEAIRDAAPGR
                     AESMYEELLNLMDMHSR"
     gene            17157..>19061
                     /locus_tag="VG272_01870"
     CDS             17157..>19061
                     /locus_tag="VG272_01870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HWF12454.1"
                     /translation="MESHTESATQLEKDPVCGMTVDPLRAKATHEHAGRMYYFCCAGC
                     KTKFIAEPAKYLMPKMLIGIAPMSAMPIQIAPAPTHPISIAQPIANIAAVPVPIAGSK
                     GASNEYICPMDPEVHQQGPGDCPKCGMALDPAVAAPPATKIEYTCPMHPEIVQDTPGN
                     CPKCGMALEPRSVTMAEEKNPELVDMTRRFWICVALTIPVLIISMSDLFPALSGWMKV
                     ISLRGWQWLEFILATPVVLWGGAPFFVRGWKSLLTRNFNMFTLIGLGTGVAYAFSVVG
                     VLFPGAFPMEFRGLEGEVDVYFEAAAAITTLVLLGQVMELRARSRTGAAIKALLGLAP
                     KTARLIRSDGSEVDLPLEDVKHGDRLRVRPGEKIPVDGVVLEGSSSVDESMLSGEPIP
                     VEKSKGARVTGATVNGTGSLVMQADRVGSETLLAQIVRMVAEAQRSRAPIQKLADVVA
                     GYFVPTVVGISALTFVVWALWGPEPRFAHGLVNAVAVLIVACPCALGLATPMSIMVAM
                     GRGASLGVLFKNAEAIELLRKVDTLVVDKTGTLTEGKPKLVSVEVAEGFHSNDVLRMA
                     ATLERGSEHPLAAAIVKGAEGRGVALAGSAEKFESLTGRGVRGLVEGHEVALVIQKLL
                     EELHSAAGPLA"
BASE COUNT         4517 a         5099 c         5078 g         4367 t
ORIGIN      
        1 cgaaaatttc ctcgtgtttt cgggagacga tgcgatcacc ttgccgctcg tggcgatggg
       61 cggaagggga attatctccg ttgcatctaa cgtgattccc gcggaaatga cgaactttgc
      121 gcaactttgc ctggcaggaa atttcgcaga agctcgtaca tggttgcgca agtggatgcc
      181 tctcctggaa gttaattttg ttgagacgaa cccgacgccc gtaaaagctg cgatggcgga
      241 aatggggctg ctcgaacctg tattccgttt gccgcttgta ccgccgcgcg tggaaagcgc
      301 cacaaaaatt cgtagcgtgc tggaatctct tgctctactc gaaagggctc acgttgccaa
      361 ccgctcctga gaaacttgaa aaacatatcg aagcactttt tgactcgaag ccggcgcgct
      421 attccgcaat cgatcaccaa ttttttcgca actttaagaa cgaattgaat gcgggccgca
      481 ttcgatccgc cgagcctgac tcttcgcgag taacgggatg gcggaccaat gagtgggtga
      541 aaaagggaat tcttgtcggc ttccgcatgg gtgtggtcgt ggatatgtcg ctggattccg
      601 tgaagcagcc attttttgac aaagattcct acccggtgcg gcagttcaat gaaggtgatg
      661 ccgtgcggat cgtccccggt gggtccagca ttcgcgatgg ctgctacatt ggccgggacg
      721 ttgtgtgcat gccgcccatg ttcgttaacg ccggtgccta cgttgacgac ggcacgatga
      781 tcgactccca cgcgctcgtc gggagctgtg cgcagatcgg caaaaattgc cacatctcgg
      841 cggcgtcgca aatcggcggc gtactcgaac ctgtgggtgc gatgcccgtg atcattgaag
      901 acaacgtgct ggtgggcggg aattgcgggg tttatgaagg aacgattgtg aagagccgcg
      961 cagtactggg cacgggaaca attctcaatc gctcgactcc cgtgtatgac atcgttcgcg
     1021 gcacagtgta tatggcgacg gaactaaatc ccttagtcat tcctgaaaac gcagtagtga
     1081 ttgccggctc acgcgctatt tcgcacggcg taggaaaaga gtggggaatc tccctttaca
     1141 ctccggtaat cgtcaaatat agagacgcta aaacggacgc taagattcaa ctagaagatt
     1201 tgctgaggta acttttgaat agcgccggct tcagcctgta gggtttggcc ttgcaaggac
     1261 taatcccgca ggctgaaacc cgcgccactt aatttcccac tacaattgaa tccgtggcaa
     1321 atagagatta cacgccatct acgcgtgagc ccgaagtcta aagccgaatc ggtaagccaa
     1381 accttgagtc aattgtgcac tcaattatca atgtccattc cgggaaaaat ctcggtcaat
     1441 gcctctaatc tccaatatat gatatagttg ttgagtcgta tatgggagtc aggggttccc
     1501 ttcacctacc aggacgttgc tgatgttaac ccctcgtgac ttcatccggt gctcactatc
     1561 gcctcagagc gaactgaatc cgggcattga cgccgcgctc tctgcagatt cccactcttt
     1621 gaaagagaga aaattatgaa gaacgtgttt gtcgggaaca tgaatttcca aacgacggaa
     1681 tcggagctgc gcgcgctctt cgaaccgttc ggacaagtga ctcgggttca cattgccatg
     1741 gaccgtgaga cgggacgtgc acgtggattc gcattcgttg aaatgcccaa cgatgccgaa
     1801 gcaacgaagg ctatgtcggg actggatgga agagaagtgg gtggacgagc tttgaaggtg
     1861 aacgaagctc gaccgaaggc tgcaggtggt ggcggtgggc cccgcggggg aagcggtggc
     1921 agtggtggtg gcggtttcga ccgtggtggg gatcgagatc gcgaccggga tcgtaaccgt
     1981 ggcggcagcg gcggacgagg cggcggcaaa ggtggccgtt tctcgaccga agattatcga
     2041 gatagtcccc ggcaaccgcg cgaacctcgc tggtaattca cgcgcaaatt tttgctgaca
     2101 aattgctagt tgtccgcacg cgcttccaca caaggagatc agtatggcta cgtcgaaatc
     2161 cacgtttcag aagcgccaaa aagagatgaa aaggcaggaa aagcaacgcg caaaagcgga
     2221 acggcgcgcg cagaaaaaac tgcttggtcc acaggatgtg gattttccga tggaaccctt
     2281 gactggtccg attatcgatc cggacatttc atttccggaa ccagacgaag aaagttagtc
     2341 taaattaatt ttagggaaga ttttcgttgc gccggcgtct tgccggcgtt tttcttttct
     2401 gccccaatcg caaaaaaacc gccggcggga cgccggcacc gcagaattct caattttcct
     2461 tgcagcaata ttctattttc aaaaaattcg ttcgacgctg aaatcagaaa tggctggact
     2521 ctacattcgt gcttccggaa cttgcaggcc ccgaaatttt atcccatgcg gtacgcttcc
     2581 aattttcaac aaaatgccgc cccacacccc aagagggaat atcgatcagc aaacgcttgg
     2641 aaagcccgtt caaataaggc tcatacattt cccgcagttc gcgcagcttc ttatctcccg
     2701 cctcattgca attggtcgac gccttgcatt ccaataacag ctcgcgaacg cgcggcagat
     2761 cgcttggcgg caggcggtcc cgccccggcg gcgatgctcg aactcgcaga acctcagcca
     2821 ggtccaccag gcagtgacgc gacatggcga acgtcaactg cgcttgccat ttcgccattc
     2881 cctggccgta cgcgatcatg agcgcagtca cgtccagcac tgtggtcatg gcggcaagcc
     2941 atgattgatt gtcatgctgc gaacggaaat aacagagcac gggatacgac aagtggctct
     3001 ccatcagctg tgctacccat agctcccaat cgcgcaaata cggggtcagg gcttcgctat
     3061 gcgcaaactg cgcgtgcctc cgcagcaatt cgccagccgt cgggggcgat cccgcccgcg
     3121 cgtccagcaa agaaatattc agttctcgct gcgagaacgc gccgtacaac gtgggcaaat
     3181 atgaaatcat gaggccaaga aaaccaaatc ccgtacctcc ttcgcaaacc gtaatcacgc
     3241 gtgccaaggc cgtgcgcggc gttacatcgc ccaatcccaa tgtaaatagc gttgtgccgc
     3301 ttagataaaa atcggtccga aatgtaggcg atccatctgt agtattaatc gcggaccccg
     3361 ccgcccaatg cagcatggcg aacccggata taagaagaag cgcccatgcg gcgaacaagc
     3421 ccaggagcga aagcgggcca tagtaactca ggtgcgcttc ccgcattttt ttcgtacgaa
     3481 atagccggtt taccgcggcc cagaagaccc atgtggttcg ataaaaaatg cggacgatac
     3541 gaaccgggcg tgtgacgcgg cggggcagaa taatcgtttc aaacgcttcc caaagcgtaa
     3601 tcagcaaaat agaaatgcca gcaattgcca ccagaatctt catttatgct cccgcgcctg
     3661 acaacacaat atcaggagtt tgttgtgagg agaaattcct gcagctcggc aaaacggttg
     3721 ggaaaaggaa ttgatttttt ctggcggttc agaattgccg cgagccgttc gggcatttcc
     3781 ggacgcacgc cggtggcgcg cgcaacggtt tcgtcaaatt tcgccggatg cgccgtggcg
     3841 agaacgattc cttgcgtggt cggaggattt tgctttgcga aactttgcca gcccagaact
     3901 cctactgcgg tgtgaggatc gagcacatat ccatggcgct cgttcacatc gtgcatcatg
     3961 cgcaaggttt cttcatcgct gaagctgcat ccccaaatat cgcgacggat ggcttgtaaa
     4021 tcgtttccgt agagatcgac aatgcgcgcg aaattgctcg ggtttccaac atccatcgcg
     4081 ttggaaattg tgtgacgtga agctcgtgga atcagattgc cgctgcgcag gaattccggc
     4141 acaacatcat tcgcattcgt tgaagcgatg aattgcgcgg tgcgcaggcc aatgcgcttg
     4201 gccaacaacc cgccggtcaa attgccaaaa ttgccgctgg gaacagcaaa tgcgggaagc
     4261 gtgccagccg ttttcaattg tgccagagcc gaaaaataat aaaaactttg tggaatcagt
     4321 cgcgcaatgt tgatcgagtt cgccgaagtg attgtaagtt tttcggcgat caccggatcg
     4381 accagcgcct gcttggcaag ccgctggcaa tcgtcaaacg acccggcgac ttccagcgct
     4441 gtaatattct ggccgagtgt cgtgagttgc ttttcctgcg cttcactcac gcgcccactg
     4501 gggtagagga tgatcacacg gatgccggcg agtcccagaa acgcgtgcgc cgccgcgctg
     4561 cccgtgtctc cggatgtcgc cacaataacg gtgagttcct tacccgattc ctgaacaaaa
     4621 tatcccatca ggcgcgccat gaatcgcgcg ccaaaatcct taaacgcgag tgttggcccg
     4681 tggaaaagct ccagaatatg gaggcgttca cttagagtga cgagcggaac gggaaaatta
     4741 aatgcctccg tcacaatctt ctgtaaaacg gggtgaggga tttcgtcgcc cgtaaacaac
     4801 gagcctactt ccatggcgat ttccggtaac gtgagcgacg gcagtcgatc cagaaaatct
     4861 ttttccagga ggggcatttc caccggcata taaagtccgc cgtcgggagc cgatccgcaa
     4921 agcacggcgt cgcgcaggga tgcttcaggg ctgcgatggg atgtgcttct atatttcatt
     4981 gagcgggccg ggcctgcgcc tcgagatatt ccagaaagcc gcgcgcatca atcgtgagat
     5041 cccacatgag gtccatcgga acgatgcggt gcgcgtaacg cttgtacatt tcttttcggt
     5101 gcgcgatcag ctgtggatag cagcgttcaa tcgtgtccgg cgtagattcg ccggcgcgcg
     5161 ggacaaattg gtcttcccac aacacgggct tcgggctcga catataacgc gcaatcaata
     5221 agttttcttc atcgggcgag gcttccagat aaaccaccgt ggtgagcttt tggaatcggc
     5281 gacagatttc atcgcccgta taaactacac tcccggttgt gtccagaatg attccttgat
     5341 tgccggatgc ctcaatctcg ttcaacgctt ctctcatggc ttccacttca taatccaaat
     5401 attttttctc gcgcgcgcga taggtcgggc tatccggcca tcccatccat gccgcaaccc
     5461 cgccaattcc tcggtatccg tccttagcga gttcgggggc aagtttttct tcaatgcgat
     5521 cgtccacacc gatcacatga aatccggcac ccgcaaattt ctgcgaccag tatgattttc
     5581 ccgcgccaga cattccgata agagaaattt taaacacaca acctcgccac gaaaatatta
     5641 cgttttgact ctgtcgccac tgaggtggga caaaattcat cactgagaca gaacacaaag
     5701 aatagcgttc cgtgatgcca cctgcaagtc cctgcgggcg ggcaggtgcc cagtttaaag
     5761 gcccgctttc agtgtttgca tgtttacaaa agtgcccggt cgaatagcgt tcggttgcgg
     5821 agcatccatg ccgctgcgcg cgtacgccat gcaagccccc acaagggcgt tgcgtcctga
     5881 ctttccggag tgacaggtac ttggatcgct tggtccgcag catcctggat tgcgaagctg
     5941 atcatcgtat ccaccaactt taatcgatca ctggtgctta atgcatagcc gtcagacatc
     6001 acgcggagct gccgcgcgcg atcttccaca ggcaataatc cttgccgtgc ggcaatgtca
     6061 tcatcgtgga gttggacatt taaccagcaa gcctgcgcga actcaaccag ctgatcaacg
     6121 gggccggcaa cttcccagtc gataagtgcg atcggctttc ccgcccgtgc aactacgttc
     6181 catggtccaa catcgcaatg cccaaacaca agttgcccat tgcctataag attgcggcca
     6241 aaccacgggc gccagatcgc attgacaggg gattcaaaag tagtggtggc tttatgtaga
     6301 gccctgatca tttctccaag tgcggcgagc ccctcatccg accaagaatg agggtggacg
     6361 aaagctccct ctatatacga caaagtttcg cgtcctttta tgtcaaaacc ggtgccgatt
     6421 actcttggcg aggccgtaaa gccgcagcat tcgaggtgac ggagtagctc atgaacactg
     6481 gacgcccatg gtcccgtttc gcggaaaact atttcaccgt cgcgtgtaac tctcgtacgc
     6541 ccgcccccgc ggagcgcgat ctctcgatca ccctcgttgc tcatcttttc gtccaattgc
     6601 gcacagaatt cctgaatcga cgggagtcta tcatttgtgg acaattattt cgtacttgaa
     6661 attaatgcaa gagaatctcg aatcacgtca taaacaaaat tgagctgctc tggcgtgatg
     6721 acatacgggg gcaggatgta aacgacgttg ccaagaggac gcaacaacac gccgcgactc
     6781 agaaaatatt ggtacaacct gggacgcagc gacgacaaat agcccgcatc gggaaccttc
     6841 aattcgatgg ctgcaacggt gccgatgtgc cgaacgtcag ccacgccagg atgagcagcg
     6901 aaagctggaa gccgttcggt atgacctttt tcgatcgccg caatccgctc aaaaaccggc
     6961 tcatgctcga aaatttgcag ggacgcattt gcagccgcgc aggcaatggg attcgcggtg
     7021 taggagtgcc catgaaaaaa agagcgcatc cggtcgccac ccacaaacgc atcgtgaatg
     7081 cgatcggtag cgaacgttgc ggccagcggc agaaatccgc cggttaatcc cttcgccacg
     7141 cacattaaat ccggaacgac agctgcacgc tcgcaggcaa acatacgccc tgttcgacca
     7201 aacccggtga gaacttcgtc cgcgatcaac aggacattgt gagcctccgt caggcggcgc
     7261 actccagcga gaaattcttc aggatggacg atcatcccgc ccgcgccctg aagcaacggt
     7321 tcgacaatta cgacggcaat ctcttcaccc tgttcattga gcagactttc caatttgttg
     7381 aggcactcaa tgtggcaagt cgagcgcgtg aggccgacgg ggcagtgagc gcacgaagct
     7441 gaatgggtgc gcaaaacagg gattcgcaaa gagtcgaatg ccgcggtaaa tggcgagtcg
     7501 tcgctgacgg acatcgcgcc taccgtgtca ccgtgatatg cgttttccaa cgcaacgatt
     7561 cgagattttt tcagccgtcc gcaattcttc caatattgca gcgccagctt tagcgcaact
     7621 tccaccgctg tggagccatc gtccgagaag aaaacatgac gcaagggagc aggaataact
     7681 ttgcccaggc ggaatgcaag ttcttcagca ggctcatgcg aaaatccggc aaacaaaacg
     7741 tgttcaagct ttgcagcctg ttcggccaca gcttgcgcaa tggccggatg cgagtgcccg
     7801 tgcaggttga cccaccagga tgaaatcgca tccagaatct gccgtccatc ctgcatttca
     7861 atgaacacgc ctttaccgcc gcgaactacg aggggtgccg ggtcgatgcc ttcctgcgtg
     7921 aagggatgcc acagatggag atcggatgcg ctcatgattt attggaatgc ctggtgatcg
     7981 aaatattttt cgtaaacttc aagcagcgtc gcgcgatgaa ttgtctcaag cagcggaata
     8041 tgcccgacga cgcgcacgtt gccatagtgt tcaattgcac ggcgattctc aacattttca
     8101 tcgccaatca tcacgactcc gcaaatcggc aagctcaatt cgcgcaacgc agcaaacgtc
     8161 agaagagtgt gattgatcgt acccagagca gtgcgcgccg cgataacgac tggaaatcca
     8221 attcgccgca tcagatcccg catcaactcg cacgtattga taggtaccat cacgccgccc
     8281 gcgccttcga cgatccattt tgcgtcggct ggcgctgctg gcaattcaaa atcatccaaa
     8341 tgcaattcaa tccccgcggc gcgcgctgcc agatgcggcg aaaccggagg atcgaatcga
     8401 tatttttctg aaggaagcag gtcctccgaa attcctgtcc acttgcgtac ggattcccga
     8461 tccgtccctt caactgcgcc cgtctgcaca ggcttccaat agtgagcgtc taatgctgcg
     8521 acgagcagcg cggagagaac ggttttccca acattggtgt cagttccggt aacgaagtac
     8581 ccgctcattg ggagacggaa acagcgcgtg cagtggaata ttcgttgcgc gcctgcacca
     8641 tggccaaaac aagttctgcc aaaatatctt ccgaatgttt tgccgtcaga gaaatgcgca
     8701 ggcgcgcttt tcctggcggg accgttggag gccggatcgc gcgcacgcca aatcctctgg
     8761 ccctcagata ttccgcgaat tgcacagcgg catcgttcca tccaaggatg atgggcacga
     8821 tttgtgaatc gatctcgggg atataaaaac cgttcgttcg caattcgttt ctgaggaacg
     8881 cgctgagttg agccagccgc gctcgcccag catccgcatc cgcggccagc ttcacgcctg
     8941 cggaaacctg cgaagcaaaa tacggcggaa gcgccgtgtt aaaaataaaa gtgcgcgcgc
     9001 gattaatcaa aagtttttgc aaattttccg acccgcatac aaatgctcct gccgaagcga
     9061 gtgctttgcc gcaagtatgt accgtagcga gtacacgcgt ggaaagtcct gcctccgcca
     9121 cgcatccgct gccgttcggc ccgcgcacgc caatcgcgtg cgcctcatcg acaattaatt
     9181 ccgctccgta gcgatccgcc agagctgcaa gctcagtcaa tggcgcgcgg tcgccctcca
     9241 tgctgaaaat actttcgacg acgatgatcc gtgcgccaga cgcggaagcg ttgcgccgaa
     9301 attcattctc caaaaattcc agattcagat gtggaaaaat aacgcgcttg cttttagtca
     9361 ggcgaattcc atcgatgata ctgacatgat ttgcgctatc cgaaaaaaca atgtcttctg
     9421 gcctcaggat ggcgctgagc agtcccaaat tcgcagcgta accggaggtg aaatacaaag
     9481 ccgcgtccgt gcccacccac ctggcaaaat cctgttccag cattgtccat gtatcggatt
     9541 ggccggacaa caatcgcgac ccggtggacg cgatgcgcgc agcatattcc acgccttccg
     9601 cgatcgcctg cttcatccgc ggatcggtgg ccaggcccag ataatcgttg gaactgaaat
     9661 cgatcccgtg aaccatttgc aattggcgca gttcggcccg ggattccagc tcagacaact
     9721 ccgcgaggat gcgtttttgg atgtccacga aaataattta ttgcagcggc aaaggctcga
     9781 gcccaagcga ttgcagcatc gccatatcgt cttgaggttc cggattggca gtggtcaata
     9841 atttttcgcc gataaaaagg gaattggctc cggccacgaa acacagtgca acggcctctc
     9901 gcgacaacgc cttgcgcccc gcactaagcc gcaccatcga agccggcatc aagattcgcg
     9961 cggttgcgat agtgcgaacg aaaaccagcg gatcgagcga gtcaacttgc tcgagcggtg
    10021 ttcccgtaac gcgcacgagc gcattaatcg gcacactttc cgggtgcgga ttgagcgtcg
    10081 aaagttgctg cagaagcccg atgcgatcgt tctccgattc gcccagcccg ataattcccc
    10141 cacagcaaac cgttactccg gcttttcgca cagaagccag tgtgcgtaat cggtcgtcat
    10201 aatttcgcgt agtaataatt tgtccgtaaa attcaggaga ggtgtcgagg ttgtggttgt
    10261 aagcggtaag ccccgcctca gccaatcgct ttgcctgcga atcgtcgagc atgccgagcg
    10321 agcagcagac ctccatgccg aactcggcca cgccgcgcac catttttaaa atattttcaa
    10381 agtcgtcgcc ctcctgaaca ttgcgccagg cagcgcccat gcagaaacgc gttgcccctt
    10441 cgccgcgcgc gcgttttgca aactccagca cttcatccac tggcaagagc ccgccgcgcg
    10501 taacctccgt cgagtagtgg gcgctctggg ggcaatagct gcaatcttcc gggcaaccgc
    10561 cagttttcac ggaaagcaat tggcaaaatt gaacgcgtgt agggtcatga aactggcgat
    10621 gtacttgttg cgccgaaaaa agaagatcgg gaagcgcctg cctgtagatt tgctgaattt
    10681 gttcgcgcgt ccagtcgtgc cgcagagcgg ctcttgctgc cgaaatgcct gcatcgttat
    10741 gcgacaaggg cactcctcta aaataaaaat ctaaagactt tgtcccgcac gcaactcaaa
    10801 aaaaccacaa ccaattgcaa gtcaacgtat ccacaaagag ttgactcggg aaacattcta
    10861 gcgcagattc aacaaaaata tcgacagtgc ccagccgaaa taatgcagat caatagcatt
    10921 tgcaatttcg cagtctggac caaaacatat gggaattgaa ggagagaatc tgtttgggag
    10981 acgctgctaa aaacttctta caggtgtgat tcctcggttc gattggtttt tttggatccc
    11041 ttgttctcat ccggggaaga agactcgatg gcatttgagt ttttcttatg acaggaaacg
    11101 gcccaccatc gattctggta ttgcggcagt tcgcacatct tggtgcaatg tttttttccg
    11161 gattcatacg cgcaatggca gtagcagaga gttgtttcgt gccccgatag cagaatcggg
    11221 acctcattgg ccagggcacc agcagagaga gctcccagga aaatcgctgc ataaacggcc
    11281 ttccggaatc cactaaatcg aaatggcatg ccagattttc aatccaattg gattaaaaac
    11341 cgatagcgcg gaagtccgaa aaaagatggt attgctggtt ggagcgtact gtcggaaagt
    11401 actgggactg gcactaccga ctacgctccg ggatttgcat gacggaaagt tgcgaagatc
    11461 ccgtctcaaa gaaatactgc gtaccgtgag gcaacgggac tacgaacgga agtctagtcg
    11521 ctaaactttt gcagaaaact taaacgctgg ccggagcggc ttcagccaag tccgacaatt
    11581 ccaaccgcaa gtttccagag tttgacgcat agctgagtgc cgattcgagg tcaatgacgt
    11641 cagaccggac caggcgctcg agttcaccat cgaagtgttg catcccctcg gtggcgccat
    11701 cgcgcatcgc atccagaagc gatttgcctt ccatttcgcc tttttccaca tattcgcggg
    11761 tgcgcatggt cgatttcaat atctcgatcg cagccactcg cccggattta tcttttttgg
    11821 gaatcaaacg ttgcgaaacg atgtagcgaa acgatctcga cagccgcgag cggattgtct
    11881 gttgatctgc gagaggaaac acgccaacga ctcgttcaat tgtttttgac gcatccacag
    11941 tatgcaatgt cgaaaaaacc agatgtcccg tctccgctgc ttccattgcg atttcaattg
    12001 tttccctgtc gcgcatttct cccacaagaa ttactttcgg ggcctgtcgc agcgccgcac
    12061 gaagcgccag ggcaaaactc ggcgtatcgc tatggagttc gcgttgatgg attgtggatt
    12121 ttttatgcaa atgcagaaat tcgatggggt cttcaatcgt aaggatgtgg caggcctttt
    12181 cttcattgat cttgttgatg atggccgcaa gagttgaaga tttccctgaa ccggttggcc
    12241 cggtaaccag cacaattcca tttcttaatc ccacgatttc gcctaacgcc gccggcaaat
    12301 ttagcgaagc gaaatccggg acagtctgag gaataacgcg catcacaatg gcgtatgtgc
    12361 tgcgttgccg aaaaacgtta actcgaaagc gcgcgaggcc cggcaaacta tacgataagt
    12421 ccgttgcccc ctctttctcc aatttggacg tcacgatttc attttggccg atcaagtcca
    12481 gggcaatccg gtgcgttact tcaggagtga gccgcccgac tcccgtgata tttatttctt
    12541 tcagttgtcc attcaactca acttgcgggg cgcggcccgg cgaaaagatc aaatcgctta
    12601 cctgctttga gacctgaagc aaggtggcca caagagccgc cgtggggaca gcaggcttcg
    12661 cttgagcttc cgctgtctta gtttgcgcgg ctgcaggatt ttcggtaagt actgagttcg
    12721 aatccaaggg tccctccgac acgttgaccg gcctaatcga ttagtttagc tggaatttaa
    12781 ttttaacttg cctggttaag cgaagttttg gtgtttttca ggaggtgtgc cttattgaaa
    12841 cactttgcag ttctttttta atcatgttta tggcctcaga tttaagcttt actttatcaa
    12901 gccttagacc aatgtccgta agttggtata ttttgtggtt atagacatgt gaagtggtta
    12961 atgaattaga taggtggaac ttgagagtga ctatgacgcc ctccgggaaa tggatcgaga
    13021 gattcttgag cttatgatcg atcttggaag atttagctag gggcaaaggc ttcaccttgc
    13081 gccccaattc cgcatggcgt ggcccgacgg cggaacagtt tctgatgttt agtaaagttg
    13141 agcggattgt ctcttttttg gcggcattct ttttcaagtc aagactcctt ctctcgtgtc
    13201 gagtgatgat gtagtgggat ggaggccttc agcttcgcgg tcgattccgc agtatggaca
    13261 aaagcgaaat aaagcagaga gctttttgcc gcagtgctca aacacgttgt cactctggat
    13321 gtccatgggt acgacgcgaa taatttcatc gagcgtggtc acccctgcct gaagtttttc
    13381 caaggcatct tcctgcatcc tttgcatgcc cgcggcacga gcggcatctc taacctgatc
    13441 gggtttataa gtgccccgca ggatggctcg tatggattcg tcgatcatca tcagctcata
    13501 aatgccgata cgccctttgt atcccgtaaa atcacatact ttgcatccct tcggccatgc
    13561 gatctgggtt ggtggattgt tccatccgac cgccatcaac cgttcggcat acgcgggtgt
    13621 gacgtcctta agcccatggc aggagcacag ctttcgtact agcctttgcg ccaggatgga
    13681 gctcacggaa gaggccacca gatattctgg aatgccgagg tccagcaatc gagccacggc
    13741 ggaaacgctg tcgttcgtgt gtaacgtgga taagaccatg tgtccggtct gagcggcctt
    13801 catggcaatc tccgcggttt cgagatcgcg aatttctccg atcatgatca cgttcggatc
    13861 ttgccgcagt atcgagcgga ggcaacttgc aaatgtcagc ccagcacgcg tgtttacttg
    13921 cacctgattg acgcccggta gcgcgtactc gatagggtct tcgacggtca cgacatttac
    13981 ggcaggcttg cgtaataaat tgagcgcagc atagatcgtc gtgctctttc ctgagcctgt
    14041 cggcccggtg atcaatagca tcccctgcgg ctgtgccaag gattgaagca gccgtgcagc
    14101 caagtttggc ggcagcccca gttcaccaag ggtggatgcc ggtgctgtgg cctctagcaa
    14161 tcggataact actttttccc catactgggt aggcagcgtg gagacgcgca tatccacacg
    14221 ggacttacca atcgccacca tgaaacgccc gtcctgcggg gcccttcgct ctccaatgtc
    14281 catgtcggcg agtatcttca atcgggatat taaggagttc tggatgttta gcggaacggt
    14341 ttcaagttcg cgcaaaactc catccacacg tatccgtatt gtcgtacaga ttgcttgcgg
    14401 ctcaacgtga atgtcgctgg catgcttggc aatagctttc tgaatgattt ccgaaaccag
    14461 gcggactgcc ggcgtctttc ttttattcag ttcggcttga atttcctgaa tcgcgtcgcg
    14521 atttgcctgg cgtgagctag tcgaaataaa ttcaacgtct tgctcgattt ccgatgaatt
    14581 gtcacctgcg gcagttgaag catttcgact attcaggacc ggttccagct gagcataatt
    14641 tcttgtaatc gctgcttgaa tttccgcgcg aaatccaaat cgcggcgata agtctttgcc
    14701 gctggtaaac cgaacttcat caattgtcgt tacattttgc ggttccgcca tggccaccac
    14761 caatcgagtc tggtccatct gaatcgggag gatgtccaaa cgttgagcca tggattttgg
    14821 aatcaattgg agagcttcct ggtcgctccg gatcgtcgcg gaatccacat aaggcacgcg
    14881 ggatacttct tcaagagctt tgaccagcga agtcttatcg accagccccg tctccagaat
    14941 caactcgccg aggcggacca tcttgctttc ttgctcttta aataatctct gtaaattggc
    15001 cgcggatatt tggccgcgag cctgcaagac ttcgcccagt ttcttgcgct tcatgcttat
    15061 gcctcgccat aagttggggc acgtaggctt ccaatgtgag tcgaaaacta cgatttaaac
    15121 ttcctgtttt cagccgctcg cgaagcggtg gaactctatg ggccaaagga attggtgcaa
    15181 tctgggaaac ggttccagtt tgcacgctgt gcgcgtcctc agcggaaagt cagggacgat
    15241 ggccgaatcg ccgttagtga agtttcgccg cgtgtgatcg tcgcgcagga tgacgcttgt
    15301 gaaccaaggg ggaatgtctc gatgcaagat cgtaactatc aattcaaaaa attcccgagc
    15361 cgggagaccg cgatcccaaa acaggatgca cgaggtcttg gaattgacaa gtgcgaaaaa
    15421 ttaacaacct gtaaacttaa gcaatttaag ggagtaactt ggataataac gcggaatggt
    15481 tttggcccgc agcgtgcact agaaagaatt cgtggacgct atccgatgga cgacttacac
    15541 aagtctttga gcgaggggga aggggcttgc tcaactacaa gtgctggtcg ttcccacctg
    15601 cctctgagag ggaagctgga tggatgaccc cacgccccgg tcaaaaaaca caccgggaaa
    15661 cggatgcgtc ttagggggtc cttgttccca agcaagggcc cccgttttta tttcaaatcc
    15721 ttgacccgcc tgattgtttc gcacaattaa aaatttctca tgctcctgac gtccgccagt
    15781 ccgtatctcg taaccccgcc ggcacattaa acccaactcg aacccatctc ataaatagtc
    15841 ttcctgggtc acggtttcag ccgcgacatg caaggatttg aaaaacatgg ccactagccc
    15901 ctgtcgcact gttgtagccc gtctcttcag aggcgggggt ttaaacgatg atctactcaa
    15961 aacctgaaat aagggtgccg atggttggac tgggcctgaa ggccccagac acaggcgctg
    16021 aacgggaaat tttggtcaca ctttcatcta aaccctcgcc tctgaagagg cgagctacaa
    16081 ttccagcacc acaaaacggg cgaaacttac tgcagtatgc aggatatcaa cggagccaga
    16141 tggcttatca atagaaatca gagatacaca tgttttgatc tgcaattggt cccgcttcag
    16201 tttgatcgtg gaattctgct agacctgcga aatcgttccc accggttgca cgcggcgaag
    16261 aacgggaatc ttcgccattc ttaacacatg ctcgacttcg gtttccttca gccgcgaagc
    16321 gtcatattca aacgacaggc ggttcgcatc ttcatcaacg cgaaagcggc gtagtccata
    16381 ggtgttggca aaaccgccta gagcgcgcaa ttgatccggc tgcaaccgcg attgcagttc
    16441 gtacgtgatt tccatgagtg tcataaattt attatagcag ttgggcgaag ggtgctccgt
    16501 ttgacatagg gggagggggt atagtataaa taccactgag gctgccttca atcgggggat
    16561 ttatggccgg aaaagcggca acaaaactta aaatcgagac atcctgcggc tgcggcacga
    16621 acgaaacaag ccgcaaagcc ctggccgtcg atccggaaat caagtcggcg aatctgaagc
    16681 gcttgcgccg cattgaaggc caggtacgag gcttgcagcg actggtgggg gaagatcgct
    16741 attgcgccga catcctgata caaatttctt ccgttcagga agctcttcgc tctgttggcc
    16801 gcgccttgat gaaaaatcat ctgcggcatt gcgcgtcgga agccatacgc gatgcagcgc
    16861 cggggcgtgc cgaatccatg tacgaagaat tgctgaacct gatggatatg cattcgaggt
    16921 aacaagcctt gggattttat tggcgcgggc ttcagcctgc gggggttcga ccgtgcaagc
    16981 tcaaaatttc gcagtctgaa gagtctgtgt gagaactgca gaatcgttgt gggtcatggc
    17041 attagccacg acataaatcc tgcgaaatga gtctggcttt agccgctggg gtttcaattt
    17101 ttgcagttct cactcagact gtgaagcccg cgctgcaaaa agcaaaaagg gaactcatgg
    17161 aatcgcatac agaaagcgcc acgcagctgg aaaaagatcc cgtctgcggc atgaccgtgg
    17221 acccattgcg cgcgaaagcg acgcacgaac acgcaggcag gatgtattac ttttgctgtg
    17281 ccggctgcaa aacaaaattc atcgccgagc ccgccaaata tctaatgccc aaaatgctca
    17341 ttggcatcgc accgatgtct gcaatgccca ttcaaattgc gccggcacca actcacccga
    17401 tttcaatcgc gcaaccaatc gcaaatattg ccgccgtacc ggtacccata gctggaagca
    17461 agggcgcctc aaacgaatac atctgcccca tggatcctga agtacatcag caaggccctg
    17521 gcgactgccc aaagtgtgga atggccctcg atcccgcagt cgcagcgccc cccgctacaa
    17581 aaatagaata cacatgtccc atgcacccgg aaattgtgca ggacacgcca ggaaattgcc
    17641 ccaaatgcgg gatggcgctt gagcccagat ccgtgaccat ggcggaagag aaaaatccgg
    17701 aacttgtgga catgacgagg cgcttttgga tctgcgtagc gttgacgatt ccggttttga
    17761 tcatttccat gtccgatttg tttcctgcgc tttcaggatg gatgaaggtc atttcgttga
    17821 ggggatggca atggctggaa ttcatcctcg ccacgccggt cgttctatgg ggtggtgcgc
    17881 cgttcttcgt gcgcggatgg aaatctctgc tcacgcgaaa tttcaatatg ttcacgttga
    17941 ttggcttggg aacaggtgtg gcctacgcct ttagcgtggt gggcgttctt tttccgggcg
    18001 catttccgat ggaatttcga gggttggaag gcgaagtcga tgtctatttc gaagccgccg
    18061 cggccatcac gacgctggtg ttgcttggcc aggtcatgga attgcgcgca agaagccgca
    18121 ctggcgcagc gatcaaagcg cttctcggcc tcgcgccgaa aactgcgcgt ttgattcgca
    18181 gcgacggctc ggaagtagac ctgcctcttg aagacgtaaa acatggcgac cgtctgcgcg
    18241 tacgccccgg cgaaaaaatt cccgtggatg gggttgtcct cgaaggctca agtagtgtgg
    18301 atgagtccat gctcagcggc gaaccgattc ccgtggaaaa atccaagggc gcgcgcgtga
    18361 caggcgcaac tgtgaacggc actggctctc tggttatgca ggctgaccga gttggttcgg
    18421 aaactcttct cgcgcaaatt gttcgcatgg tagctgaggc gcagcggagc cgcgcaccga
    18481 ttcagaagct cgccgatgtg gttgccgggt atttcgttcc caccgtggtg ggtatctcgg
    18541 ctttgacgtt tgtggtttgg gccctttggg gacctgagcc gcggttcgcg catggtctgg
    18601 tcaatgcagt cgccgtgctg atcgttgcat gtccctgcgc ccttgggctt gcaacgccca
    18661 tgtccatcat ggtcgcaatg ggcagaggcg cgtcgcttgg cgttttgttc aaaaatgcgg
    18721 aagccatcga gctgcttcgc aaggtggaca ctcttgttgt ggacaaaacc ggcacgctca
    18781 cggaaggcaa gccgaaactc gtttccgtgg aagttgctga aggatttcac tcgaatgatg
    18841 tgttgcgcat ggcagcaacg ctggaacgcg gcagcgaaca tcctctggct gcggccattg
    18901 tcaagggcgc agaggggcgc ggggtagcac ttgccggttc agcagagaag tttgagtcgc
    18961 tcacaggccg gggagttcgt gggcttgtcg aaggccacga ggtagcgttg gtaattcaaa
    19021 agctgctgga agagttgcac tctgccgccg gtccgctcgc c
//
LOCUS       DASZMB010000041         3610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_31708, whole genome shotgun sequence.
ACCESSION   DASZMB010000041 DASZMB010000000
VERSION     DASZMB010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31708"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VG272_01875"
     CDS             <1..272
                     /locus_tag="VG272_01875"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWF12455.1"
                     /translation="EAARKGVRVNAICPGPVTETKMSKDLGQTLATRLGVSKEDQLKG
                     FLSTILQGRGQTVNEIAAAALFLCSDEASAITGQALNVDGGAAFC"
     gene            complement(850..2649)
                     /locus_tag="VG272_01880"
     CDS             complement(850..2649)
                     /locus_tag="VG272_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWF12456.1"
                     /translation="MATSSLPRAASRGGSFLVESCTPEDVFSPAELTDDQKLIGQTAE
                     EFVRNEVVPVIPDLELHKSGLIAQLLKKAGEIGLLGGGVPDEYGGAGLDKVSSVQLAE
                     KLSAYASFGVSHGGHSGIGTLPIVFFGTDEQKKKYLPVLATGEKLAAYCLSEPQAGSD
                     AQNSRTRAVLSPDGRMWILNGQKMWITNGGFADVYIVFAKVDGEKFSCFIVERGFPGF
                     SAGAEEKKMGLKGSSTTAIFFENCQVPKENLLHEVGRGHIVAFNILNAGRFSLGAYCT
                     GGSKDTLAIASKYAKERVAFGRPIAEFGLIRAKLAEMAIRIFAVESMIYRSAGLMDRA
                     IDAAGENADKSLQSMKVYEEYAIESSISKVYGSELLDFVVDEAVQIFGGYGFHEDYPV
                     ARSYRDSRVNRIFEGTNEINRMLIIQMLMKRATTGVLPLMQAGAKLREEIMAGPSFDE
                     PETGPWVEEEKILAGIKKAFLLVAGAALEKYRERLADQQEIVAALSDIVMECYAAESS
                     LRRVQKAEAARGAASATVMADAARVFLHDAADRVEVGARKALAAVLDGDMLRTQLAVL
                     RRFMKREPVDTLAMRQRVSAAVQANDRYPFEGR"
     gene            complement(2697..3152)
                     /locus_tag="VG272_01885"
     CDS             complement(2697..3152)
                     /locus_tag="VG272_01885"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HWF12457.1"
                     /translation="MASRTVWHFAYGSYMNRAQMLSRAGNILEERNASLPNYEVRFNK
                     KVRGGTAGANIQMASGKTVHGVLYKVEESALRSLDRYEGVPEHYRRIEVQVTPEGGQP
                     VPAQIYIASKVEKGLRPSAPYLQAMLDGAGEHNLPASYIGEIKSAAGAA"
     gene            3368..>3610
                     /locus_tag="VG272_01890"
     CDS             3368..>3610
                     /locus_tag="VG272_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12458.1"
                     /translation="MPNSRIRNNRLIGTVLWAAILVLLASISGPIEAAPGNGSQGSAP
                     AGEQQSRITYVTIAGSEPAGSFGGVAYKRTWGTVAGV"
BASE COUNT          851 a         1011 c          943 g          805 t
ORIGIN      
        1 ccgaagcggc ccgcaagggc gtgcgcgtga atgcgatctg tccggggcct gtcacggaga
       61 cgaaaatgtc aaaggactta gggcagaccc tggcaacccg gctgggagtt tcaaaggaag
      121 atcagcttaa aggcttcctg agcacgatcc ttcaaggccg cggccagact gtcaatgaga
      181 tcgccgcggc agctcttttt ctttgctcag acgaggccag tgcgattact gggcaggctc
      241 tgaacgtgga cggcggagcg gctttttgct gatctcttcg gctgcggtaa tcgcgcgctt
      301 ggcctgattc ccggctatgc ccaatcccca tcggctggcc cgatcaatca ggtacaagac
      361 caaagcgaac acggcgcaaa cgatgaagat gcccgtgaat aaaatcagag cagctaaaag
      421 ttcacgcaca aagtaaattt gtaacgccgc aaaaaccccg aggccgatcc acaaccactt
      481 tcgagcctta agttcggcaa taacatcact taccttcaat gagagtcccc cctataacgg
      541 acatggccat tggatgcaat tcaccgccca gaagcgtcaa gtttggcgaa aatcacaagt
      601 cattcttttt cttgagaata tgcgaatagc agataggtgc aaaacgaaat gaggcaatac
      661 tagtactgag ttaattagaa cttctatggg aattcatacc acttttttgc agttccgcag
      721 agacacactt cccttcattg ttctgaaacg gcgaaattgg aatattgttt tacagggacg
      781 gaaaactgtc acggcgcatg actgctacga gcaaataaat ttgagcaagt ctcgcgcgag
      841 gaaaccgctt cagcgtcctt caaacgggta gcgatcattc gcctgcaccg ccgcgctgac
      901 gcgttgccgc atcgcgagag tgtcaacggg ttcgcgcttc atgaaacggc gcagcacggc
      961 gagctgtgta cgcagcatgt cgccatcgag cacagcggcc agtgcctttc gcgcgcctac
     1021 ttccactcga tccgcggcat cgtgcaaaaa cacgcgcgcc gcgtcggcca tcacggtcgc
     1081 tgatgccgcg ccgcgagccg cttccgcttt ttgtacacgc ctcaacgaag attccgcagc
     1141 gtaacattcc atgacgatat cggaaagcgc agcgacaatt tcctgctggt cggctaaacg
     1201 ctcgcgatat ttctccagcg cggctcccgc gacgagcaaa aatgcttttt taattccagc
     1261 cagtattttt tcttcttcca cccatgggcc ggtctctggc tcgtcaaacg atggcccggc
     1321 catgatttcc tcgcgcaatt tcgctccggc ttgcatcaat gggagaacgc cggtcgtagc
     1381 gcgcttcatc agcatctgaa tgatgagcat gcggttgatt tcgttggtgc cttcaaagat
     1441 gcggttcacg cgggaatcac ggtaggaacg tgccaccgga taatcttcgt gaaagccgta
     1501 gccgccgaag atttgaaccg cctcatccac cacaaaatcg agcaattccg aaccgtacac
     1561 tttgctgatc gagctttcaa tcgcgtattc ctcatacact ttcatcgact gcagtgactt
     1621 atccgcgttt tcgccggcag cgtcaatcgc tcgatccatc aggcccgcag agcgatagat
     1681 catcgattcg acggcgaata tgcgaatggc catctccgca agcttcgcgc gaatgaggcc
     1741 gaattccgcg atgggcctgc caaatgcgac gcgctctttt gcatatttcg atgcgattgc
     1801 cagcgtatcc ttcgagcctc cggtgcaata agcgccgaga ctaaaacgtc cggcattcaa
     1861 aatattgaat gcgacgatgt gcccgcggcc cacttcgtga agcagatttt ccttgggcac
     1921 ctggcaattt tcaaaaaaga tggctgtggt cgagctgccc ttcagcccca ttttcttttc
     1981 ttccgcgccc gcggaaaatc ccggaaaacc gcgctcgacg atgaagcagg aaaatttctc
     2041 gccatccact ttcgcgaaaa caatgtagac gtccgcgaat ccgccgttgg tgatccacat
     2101 tttctggccg tttaaaatcc acatacgtcc atcgggagaa agcacggcgc gcgtgcgcga
     2161 attttgcgcg tccgatcccg cctgcggctc ggataagcaa taggccgcaa gtttttcgcc
     2221 ggtcgcgagc acaggcaaat attttttctt ttgttcatcg gtgccaaaga aaacgatagg
     2281 aagagtgcca atgccggaat ggccgccatg gctgacgcca aacgaagcgt aggcggacaa
     2341 tttttccgca agttgcacgc tcgaaacttt atccagaccc gcgccgccgt actcatccgg
     2401 cacgccgccg cccagcaatc cgatttcgcc ggcctttttc aaaagctggg caatcaatcc
     2461 cgatttgtgc agttcaaggt caggaataac gggaaccact tcgttgcgaa cgaactcttc
     2521 ggccgtctgt ccgatcagct tttggtcgtc cgtgagttca gcaggagaga aaacatcttc
     2581 cggagtgcac gattcaacaa ggaaactgcc tccacgggat gcagcccgcg gcaacgacga
     2641 agtagccatg gtccctcctg tatggtgaaa ggaatcttat gcagcggcgc gggcgatcaa
     2701 gccgctcccg ccgcgctctt aatctcgcca atgtaagagg ccggcaggtt gtgctcgccc
     2761 gccccatcca gcatcgcctg cagataggga gcggatgggc gtaaaccttt ttccactttc
     2821 gacgcgatat aaatctgcgc cggaacaggc tgcccgccct cgggcgtcac ctgcacttcg
     2881 atgcgccggt aatgttccgg cacaccttcg tagcgatcga gcgaacggag cgcgctttct
     2941 tccactttgt aaaggactcc gtgtacggtt tttccggacg ccatttgaat attggctccg
     3001 gccgtgccgc cacgaacttt cttgttaaag cgaacttcgt aattcggaag agaagcgttg
     3061 cgctcttcca aaatatttcc ggcgcgtgaa agcatctgcg cgcggttcat gtaactgccg
     3121 taagcaaagt gccagactgt tctagaagcc atattgcctt tctcgataaa cgggacggat
     3181 tgagcgggtg cggtgtgaac ctctattgta acgggcttac cggacgagag caaatagtac
     3241 aagatcagtg tgtaacaggg tggaaatacc accatcgcac aattgtcggt gctcgcgcgg
     3301 acttggtata aactaatttt tgaacgacac agtaccttgc gcatgcaaaa tttgggaggg
     3361 agcctcgatg cccaattcac gaataaggaa taaccggctc atcggtactg ttctatgggc
     3421 tgcgatcctt gtcttgcttg cttcaatttc aggaccgatt gaagcagctc ctggaaatgg
     3481 cagccaggga agcgcgcccg cgggtgaaca acaatcgagg attacttacg tcacgattgc
     3541 cggatcggag ccagcgggat cgttcggggg agtcgcatac aaaaggacct ggggaactgt
     3601 tgcaggggtt
//
LOCUS       DASZMB010000042         3438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1685685, whole genome shotgun sequence.
ACCESSION   DASZMB010000042 DASZMB010000000
VERSION     DASZMB010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1685685"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VG272_01895"
     CDS             <1..597
                     /locus_tag="VG272_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HWF12459.1"
                     /translation="VYRFLAVRLHPDNPETGDAEKFFLLKQAYDVLSNPARRTEYDAA
                     FKKDGPQPVPLSTSIDFMDSLEGELNRRLAILALLYIQRRTNPYFPEVSLLDVEKHMG
                     FPRDSLEFTTWYLRNKGYITRSDNSDFMLTAEGVDFVESQRVNIPILNKLLTTGSGPL
                     ATDGSSGQKVLNSSPTPIIVPASHDESGGNEKTEDVKA"
     gene            801..1718
                     /locus_tag="VG272_01900"
     CDS             801..1718
                     /locus_tag="VG272_01900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HWF12460.1"
                     /translation="MRILVSGSTGFLGTAIVDSLQKQNHLVARIVRPGSAGRNKAADP
                     RQEVSWDPIAGHFDAASAEESDALIHLAGASIADGRWSDSRKKVLRTSRIDATRHLMG
                     ALGKLQSPPRIIVAASAVGYYGSRGDETLTEQSAPGSDFLAELCREWEAETARGAEFG
                     ARVVSPRFGIILSAHGGALPRMALPFKLGAGGRLGDGQQWMSWLTLAEVVNIVQFALA
                     NSQLAGPVNAITPNPVRNSEFTTVLAKTLYRPALFPAPAFALRLALGEMADGLLLASQ
                     RVIPSRLISAGYQFQQPDLAEALADVFRK"
     gene            complement(2094..2459)
                     /locus_tag="VG272_01905"
     CDS             complement(2094..2459)
                     /locus_tag="VG272_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12461.1"
                     /translation="MPRPEAAHRVKSYSAATGFVYQYYFYEVEKTKRGGAEGTEYVYM
                     ASVDRKHVFPVKIFVVKDALEKWSARTGRAFTGTEEYAVAKMRLFQAFDELEGLAEKT
                     PELIVNESNLEALSSQLDL"
     gene            2785..>3438
                     /locus_tag="VG272_01910"
     CDS             2785..>3438
                     /locus_tag="VG272_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12462.1"
                     /translation="MNGTPKLGGQRSTGQILLCCCPPLLLVSSIVGCGAVGAGPSQPP
                     SNITVTVAPSTASVLLGEQQTFVATVSNTANTDVIWTANGISGGNATVGTINAGGTYT
                     APGIILTPPSVNLMATSAADPSKSANATINLTDTFLLSIAGATSVNAGTSATYAATLT
                     PAVNSNPSRVISWSITGTGCAANSCGAITQAGVYTAPAIPPSPATVKIIATPQADPTK
                     "
BASE COUNT          775 a          972 c          896 g          795 t
ORIGIN      
        1 gtttaccggt tcctggccgt tcgccttcac ccggacaatc cggagacggg tgatgccgag
       61 aaattctttc tgctcaagca ggcatatgac gtcttgtcga atccggcgcg ccgcaccgaa
      121 tacgacgcgg ccttcaaaaa agacgggccg cagcccgtgc ctctttccac gtccatcgat
      181 ttcatggaca gcctcgaagg agaattgaac cggcgtttgg cgattcttgc gctcctgtat
      241 attcaacggc gcaccaaccc ctattttcct gaagtatcgc ttctggatgt cgaaaagcac
      301 atgggatttc cccgcgattc cctggaattt acgacctggt atctgcgtaa taagggctac
      361 atcactcgat cggacaattc cgattttatg ttgaccgccg agggcgtgga cttcgtggaa
      421 tcacagcgcg tgaatattcc gattctcaat aaattgctga ccaccggctc tggacccttg
      481 gccacggatg gatcgtccgg gcaaaaggtt ttaaattctt ctccgacccc gattattgtg
      541 ccggcaagcc atgatgaatc cggcgggaat gaaaagacag aggacgtaaa agcctaagag
      601 cttccaggac gaagcttctc actggttcta tctatccacg aatttttaga ttgcgattcc
      661 ccgcgcctgt gaaaatccgc gctaattaca atcatttctt attcgtcgat ctgcgcgaca
      721 ttcccgagtg cgcattcttt gaatctgttt gacgtggaag gtacccgcgc cgtagcgtgt
      781 aaacacagga gaccttccca atgcgcatac tcgtttccgg ctcaactgga tttttgggaa
      841 cggcaatcgt ggattcgctg caaaagcaaa accacctggt cgcacgcatc gttcgacccg
      901 ggtcagctgg aagaaataaa gcagccgatc cccgtcaaga agtgagctgg gatccgatcg
      961 cgggacattt cgacgcagct tcagcggaag aatcggatgc gctgattcat ctcgcaggcg
     1021 cttccattgc cgatgggcgg tggagcgatt cgcgcaagaa agtcctgcga acgagccgca
     1081 ttgacgccac gcggcatctg atgggagcgc ttggtaaatt gcagagcccg ccgcgcatca
     1141 tcgtggcggc gtctgccgtc ggttattacg gcagccgcgg cgacgaaact cttaccgaac
     1201 aaagcgcgcc cgggagtgac tttcttgccg agctttgccg tgagtgggaa gcggaaactg
     1261 cgcgaggagc cgagttcggc gcgcgcgtcg tgagcccgcg cttcggaatc atcctttccg
     1321 cgcacggtgg agcgttgcca cgaatggcgc tgcctttcaa acttggcgcg ggcgggcgac
     1381 tcggagatgg gcagcagtgg atgtcctggc tgacactcgc ggaagtcgta aacatcgtcc
     1441 aatttgcgct ggcgaactcg caactggcgg ggccggtcaa tgccattact cccaaccccg
     1501 ttcgaaacag cgaattcacc acggtgctcg cgaaaacact ctaccgtccg gcgctttttc
     1561 ctgcgccggc gtttgcgctt cgcctggcgt tgggagagat ggcggacgga ttgctcctcg
     1621 caagccagag agtcattccc tccaggctca tcagtgcggg ttaccagttt cagcagccgg
     1681 atctggccga ggcgcttgcg gatgtgtttc gcaagtaagg catggtggaa actttccgtt
     1741 cgaagagaaa tcttttctac gaacagaaaa tcacagcatt tttactcgat tgcgattgaa
     1801 ctttcgtcgc gtcctgatag ccttggagtc agcagaattg tgttttgcat atataacctt
     1861 tgcgtcattt ccgaaattca ggcttccccg gcgatttaat tcgttcggtt gccatccaac
     1921 tgatgacctg ctcccgctaa atgcagaaga gtctacttga aaacttagat tggcacgtgt
     1981 agcggccgcc tgaaggcggg cgctacaaaa ctgtgtcaaa attcgctcga caccagcctc
     2041 ctcgcgcttg acgcatacgc cactgaattt tcgatttttt agttgggaga agcttacagg
     2101 tccaactggg aggaaagcgc ttccaggttc gattcattga cgattagttc gggagtcttt
     2161 tcggctaaac cttccaattc gtcgaaggct tgaaacaggc gcatcttcgc tactgcgtac
     2221 tcttccgtgc ccgtaaacgc acggccagtc cgcgcggacc atttttccaa cgcgtccttg
     2281 actacaaaaa tctttacagg aaatacgtgc ttgcggtcca ccgatgccat gtacacatac
     2341 tcggttcctt cggcgccgcc gcgctttgtt ttctccacct cataaaaata atattggtaa
     2401 acgaatcccg ttgcggcgga atagcttttc acgcgatggg cggcttcggg acgaggcata
     2461 cagggattat tcaagcgcag ggccgcggtg tcaactcagg atctgtcagc gccatccaca
     2521 caatgaattc gatgcctgcc cgaattgaat cacgattccc aatttaaatt cccatggcgg
     2581 cacgcttgcg tttcgcgtca agaaacgaat ggcgccatga atactccacc gcgtaattga
     2641 atttcggaag gcagacatcc tcaacacgat gaatgcgtga gccttgcgga aaatccatgc
     2701 agtcttaatc tgcaaaaaac tttttaagac atctctgttt tcacgctgca ccgcgttggc
     2761 acactgcgtg caatccccac ctcaatgaac ggaaccccaa aacttggcgg acaacgcagc
     2821 acaggtcaga ttttgctgtg ttgctgtccg ccattgcttc ttgtttcatc aattgtaggc
     2881 tgtggcgcgg tcggagcagg cccgtcacag cctccctcca acattaccgt gacggtcgcg
     2941 ccatcgactg cgtcggtcct gttgggcgag cagcaaactt ttgtcgccac cgttagcaac
     3001 acggccaata ccgacgtgat ctggaccgcg aatggaattt ccggtggcaa tgccacagtg
     3061 ggaacgatta atgcgggcgg cacgtatacc gcaccaggaa ttattctgac gccgccttct
     3121 gtcaatttaa tggccaccag tgcggccgat ccatccaaat ctgcgaacgc cacaattaac
     3181 ctcaccgaca catttttact ttccatcgcc ggagcgactt ccgtaaatgc gggtacatcc
     3241 gcaacgtacg ccgcgacgct tacgccagcg gtgaactcaa atccaagtcg agtgatttcc
     3301 tggagcatca cgggaaccgg ttgcgcagcg aactcgtgtg gagcgattac gcaggccggc
     3361 gtgtatacag cgcccgcgat tccgccgtca cccgctacag taaaaatcat cgccacaccg
     3421 caggcagatc cgacgaag
//
LOCUS       DASZMB010000043         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4173183, whole genome shotgun sequence.
ACCESSION   DASZMB010000043 DASZMB010000000
VERSION     DASZMB010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4173183"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VG272_01915"
     CDS             <1..563
                     /locus_tag="VG272_01915"
                     /inference="COORDINATES: protein motif:HMM:NF024767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF12463.1"
                     /translation="NMSNFNEAKKFFARSAKADKKYSSAVNNLGMVYYEQKDYRHAIR
                     QYKKAAVIDPNQAGSHANLGFAYYKMKKYAESAAEFHKALEIDPHSLDRNERVGTMVQ
                     DRSVENHGMFFYMMAREYARMGDAAHCADYLRKSHDEGYKDAAKAQTDPGFKKVVNDP
                     AVQAVLLLLVPSQNKSNQATPGSPGT"
     gene            628..>2020
                     /locus_tag="VG272_01920"
     CDS             628..>2020
                     /locus_tag="VG272_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF12464.1"
                     /translation="MSLRFFRIWLLMAGAVFGAFTIARPALASQSGNVPNSSTEGRSD
                     TILILPFENYSRIPTLDWLGEGLSEISVERLQDKGLNVLSRQERLAVLEKIGLPDSAR
                     FSHATMVKIAGEADADDVIFGKFTSDGNTVTLEANLLHISPPRLSSPLKESGALSDLL
                     RIQARLSWKILCVLNEKSCPRQVANRDETTFTEPPPSLRPEVLEAFIHGLTGPNDDLR
                     LRALRDAARLEPAWDRPAFELARIYFSRHDCEVALPWFSHVPPDRPDGPEASFDTGIC
                     HLYRNDSIRAEATFTGLLERAHSENLKDRIPELPEVHNNLGVARMNLGKWNEAAIEFE
                     RAAAMDDGEADFWVNLAIAKLAQKQQTAAVSALEHARKIDPQDKGARALLISTLESLG
                     RGSEAGAVRTESPETSSAAPTPLPQDVADLAHLGRLSKEFDRSGLRSPSDVASGQTPQ
                     NKETHKPASGGGLN"
BASE COUNT          470 a          552 c          570 g          428 t
ORIGIN      
        1 tgaacatgtc gaacttcaat gaagccaaaa agtttttcgc gcgctctgcg aaggccgaca
       61 agaaatattc cagcgcagtg aataatctgg ggatggtgta ttacgaacaa aaggactacc
      121 gccacgccat ccggcaatat aaaaaggcag cggtaatcga tcccaatcag gccgggtcgc
      181 acgccaatct gggtttcgcg tattacaaaa tgaagaaata cgcggagtcc gccgcggaat
      241 ttcacaaagc gctggaaatc gatccgcact cgctggatcg caatgaacga gtgggcacta
      301 tggtgcaaga ccgttccgtg gaaaatcacg gcatgttttt ttacatgatg gctcgcgaat
      361 acgctcgcat gggagacgcg gcgcattgcg ccgattacct gcgtaaatcg catgacgagg
      421 gctacaaaga cgctgccaaa gctcaaaccg atccaggttt caagaaagtc gtgaacgacc
      481 ccgcggttca agccgtactg ctcctgctgg ttccttcgca gaacaagtcg aaccaagcaa
      541 cgcctggttc accgggtaca taggtggcac atctcggccg cgcactgcga acatgccttc
      601 atcaagctct gctacaatgt tgaaataatg tcattgcgat tttttcggat ttggctgctg
      661 atggcgggcg cggtatttgg cgcgttcaca atcgctcgcc cggcccttgc aagccaaagc
      721 ggcaacgttc ccaattcctc caccgaaggc cgttccgata cgattctgat cctgccattt
      781 gagaactaca gccgtattcc cactctggac tggcttggtg agggtctatc ggagatcagc
      841 gtagagcggt tgcaggacaa gggtctgaac gtgctttcaa gacaagagcg gctggcggtt
      901 ctggagaaaa tcggtttgcc tgactcggca cgcttttcgc acgcgacgat ggtgaaaatc
      961 gcgggcgagg cagatgcgga cgacgtgatc ttcggaaaat tcacttccga tggaaatacc
     1021 gtaacgctgg aagcaaatct attgcacatt agtccgccca ggctttcgtc tccgttgaaa
     1081 gaatccggtg cgctcagcga tctgctacgg atacaggcgc gattatcctg gaaaatcttg
     1141 tgcgtattga acgagaagag ctgcccgcgg caagttgcaa acagggacga gactactttt
     1201 acagaacctc ctccgtcgtt gcgccccgaa gttcttgagg ctttcattca tggactcacc
     1261 ggtcccaacg atgatttgcg tttgcgggcg ctgcgcgatg ccgcacgcct ggaaccggca
     1321 tgggatcgtc cggcgttcga gctggcccgc atttattttt cgcgtcacga ctgtgaggtg
     1381 gcgctgccct ggttttcaca cgtgcccccc gaccgccccg atggtcccga ggccagcttt
     1441 gacacgggaa tctgccattt gtatcgcaac gattcgatac gcgccgaggc gacgtttacc
     1501 ggattgctgg aacgcgctca cagcgaaaat ttaaaagacc gcatcccgga attgccggaa
     1561 gttcataaca atttgggggt cgcgcgtatg aatttgggaa aatggaatga ggcggccata
     1621 gagttcgaac gcgccgcggc gatggacgac ggcgaagccg atttttgggt gaacctggcg
     1681 attgccaaat tggctcagaa gcaacagact gccgcagttt ctgcgctgga acatgctcga
     1741 aaaatcgatc cgcaagacaa aggtgcgcgc gcattgctga tttccacgct ggagtctctg
     1801 gggcgcggtt cggaagccgg cgcggtgcgc accgagtcgc ccgaaacttc ttccgctgct
     1861 cccacacctc tgccgcaaga tgtagcagac ctggctcatc ttgggcgcct ctcgaaggag
     1921 ttcgatcgct ccggtttgcg ttccccgagc gacgttgcga gcgggcaaac gcctcaaaac
     1981 aaggagaccc acaaacccgc aagcggcggg gggctgaatt
//
LOCUS       DASZMB010000044         4247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3607901, whole genome shotgun sequence.
ACCESSION   DASZMB010000044 DASZMB010000000
VERSION     DASZMB010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3607901"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..906)
                     /locus_tag="VG272_01925"
     CDS             complement(<1..906)
                     /locus_tag="VG272_01925"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HWF12465.1"
                     /translation="MLSAFLIALREGVEAALVVGIVLVYLNRTGRATLVRWVWGGVFA
                     AVASSLAIAIALERWKVSEDGFEGLMLLVAAVFVVTMIIWMNRVARHLKAEIEKRVEI
                     HASKAEFSAGLGLATFVYLMVVREGAELALILRAVELSTEGVAVWIGTVLGLAVAVAV
                     GLFFFQGTLKISLGKFFSATSTILIIVAVQLALTGIHELSEAMWLPSSRVEMAYVGPI
                     VRNEFFFFTVILGAAALLGLREWIAARGQAAENLPPDAADRRRLEWERRKQRRWALAS
                     AFTCTAVILILAAEFAYTRVTATTEA"
     gene            1242..>4247
                     /locus_tag="VG272_01930"
     CDS             1242..>4247
                     /locus_tag="VG272_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HWF12466.1"
                     /translation="MVARHFAVLAIAGLLPIIPAFSQTSTGSTSHAAVSAQVEKPDVP
                     APKPDKSRARNAFEQGRRAEQAGDWKAEYADYSEAATYDPSNHQYAMLKEHARFQVVQ
                     SLVDAAERQEIAGNTPGARALLNEALQIDPNYIVARERLQELTPAEQEVEAAKGPRLG
                     GLQRLIVKPGMQTFDFRGTTRGAYEEIGKQFGVKMIFDGDLPDRAVRFTVPSLDFDTA
                     VKILAQETRTFTRVVDEHTLFVTEDTPQKVRDYTIEVQKELLLPASVSTDEMNETVRM
                     IREMTGITRTQLNTATRTLTVRSSEQNVALAQTLLEQIEQPHGELMLEIEILELDRTA
                     ANQLGITPPTASSVFALTVPEIRQLQAAQNSGSLIQTLQSIFGSSSVSSATGSALPAL
                     IAFGGGKTIFLATMPGASANFGTALSTVQSAQRVLLRAQDGKPATFFVGDRYPIDLGL
                     LSNSLTSNSAALTQALLGGVTLPRTDYDTGNAPVSVAIATFNTKNSDHPDLAIANQTS
                     GTITILAGNGDGTFVAQASTCTPPSCIALPSVITGTTTTAASPSSIVAGDFNDDGFAD
                     IAVTDQANNRVLVLLGNGDGTFQTPVAYPTGTKPVALIAQDFDGDSQADLAIVNQADG
                     TVTILLGNKVNGTQNGTFGTPPNQTAALAVGAAPSAIATGQFNISNNANADLVVTNST
                     DNTVSVLLGNGDGTFGAQRVSNTGKGPAGVATADFNGDTKPDLAVTNQTDSTVSILLG
                     NGDGTFQTQTTFATGSSPVGIVAANFTSANSDLVVADETANNADVLIGNGDGTFNTPI
                     SLPTGNAPIALATADLNGDSTLDLVTANNASNTVTVTLNTLPSSTNPASSQVGYPSAQ
                     YEDLGLKVKATPRLHGDDEVTLQLQFDIKSLTGTSINGIPILSNRNIEQTIRLRENET
                     SVLSGILQSNKSTTSSGLPWTSTAPGIGLLTGEDTSNNQQSELLILVTPRALRVPPHE
                     GPAVYAGRGEPSSPPLGPQLPAGIPQPGQP"
BASE COUNT         1031 a         1241 c         1129 g          846 t
ORIGIN      
        1 agcttcggtt gtagcggtca cgcgcgtata cgcgaattca gcggctaaaa tcaggataac
       61 tgccgtgcag gtaaatgccg aggccagcgc ccagcgacgc tgcttacgcc gctcccattc
      121 aaggcgccgt cgatccgccg cgtcaggcgg aagattttcc gccgcttgtc cgcgtgccgc
      181 gatccactcg cgcaggccga gaagcgccgc cgcgccgaga atcacagtaa aaaagaaaaa
      241 ttcattccga acaatcggtc caacgtaggc catctccacg cggctggaag gcagccacat
      301 ggcttcgctc agttcatgga tgccagtcaa cgcgagttgc acagcgacga tgatcagaat
      361 ggtactggtt gcggagaaaa acttcccaag agaaattttc agcgtgccct ggaaaaagaa
      421 caagcccacg gccacagcaa cggcaagacc cagaaccgtt ccaatccata cagcgacgcc
      481 ttccgtggaa agctccaccg cgcgcaaaat caaagcaagt tcggcgcctt cgcgtacaac
      541 cataaggtag acaaatgtag cgaggccaag accggcggaa aattcggctt tagaagcatg
      601 aatttcgacg cgtttttcga tttccgcttt cagatgccgc gccacgcggt tcatccagat
      661 aatcatggtc acgacaaaca cggccgcgac gagcagcatc aacccttcaa atccgtcttc
      721 gctgactttc catctctcca gcgcaatggc gatcgccagg ctggaagcaa ccgcggcaaa
      781 cacgcctccc cacacccagc ggacaagggt tgcgcgtccg gtacggttca agtaaacgag
      841 gacgattccg acaaccaggg cggcttcgac accttcacga agggcaatga gaaatgcaga
      901 gagcaactag cagtccttaa gcgaaatttt ttcggcgtta cgcgacattt caaaaggtcg
      961 cctctcgccg taggggcacg acatgtcatg cctttacacc cgcgagcgcc ccgcctagag
     1021 acaaagcttg cacacaaaat ggggcccggg gacacccctc tagttgcaac tcattcgcaa
     1081 ctggaaatac tagtgtacac gtcctgattc acgcaggtca agattacaag tttctaccgc
     1141 tgcgtacgtc ccctgacggt agaataggca tctacatgat tctagctatg atcgagcctc
     1201 ggtgtcatct tgcaaatcgc tccctgtctg accgagtagt tatggtcgcg aggcatttcg
     1261 cagtcttggc catcgccgga ttgttgccca tcatcccggc tttctcccaa acttctacag
     1321 gctctacctc ccacgcagca gtctccgcgc aggttgagaa gcctgacgtc cccgctccca
     1381 agccggataa gagccgtgcc cgcaatgctt tcgagcaggg ccggcgcgca gagcaggcgg
     1441 gagactggaa agccgagtat gcagattatt ccgaagcggc cacctacgat ccctccaacc
     1501 accagtatgc aatgctgaaa gaacatgccc ggtttcaagt cgtccagtcg ctcgtggacg
     1561 ctgcggagcg gcaggaaatt gctggaaaca cacctggcgc ccgcgcgctg ctgaatgaag
     1621 ctctgcaaat tgacccgaat tacattgtgg cgcgggagcg gctccaggag ttgactcccg
     1681 cggagcaaga agtggaagcc gcaaaggggc cgcgactcgg cggattgcag cgattgattg
     1741 taaaaccggg aatgcagacg ttcgattttc gcggcacaac gcgcggggcg tacgaagaaa
     1801 tcggcaagca atttggcgtg aagatgattt tcgatggaga tttgccggat cgcgcggtcc
     1861 ggttcaccgt gccaagtttg gattttgaca ctgcggtgaa gatcctcgcg caggagacca
     1921 gaactttcac ccgcgttgtc gacgagcaca ctttatttgt gactgaagat actccgcaaa
     1981 aagttcgcga ttacacgatt gaagtgcaga aagaactttt gcttcccgca tcggtcagca
     2041 cggacgaaat gaacgagacg gtccgcatga ttcgcgaaat gacaggaatc acgcgaacgc
     2101 agttgaacac ggcaacgcga accctcaccg ttcgcagcag cgagcaaaac gtggcccttg
     2161 cgcaaacgtt gctcgaacaa atcgagcagc cacacggcga actcatgctg gaaattgaaa
     2221 tcctcgagct tgaccgcacg gccgctaatc aattgggaat tactcctccc acggcttcca
     2281 gcgtctttgc cctgacagtg ccggaaattc gccagctcca ggccgcgcag aatagcggca
     2341 gcctgattca aactctgcaa agtattttcg gcagcagctc ggtgagttcc gccactggat
     2401 cagcgcttcc cgcgctgatt gcattcggcg gaggaaaaac tatttttcta gccaccatgc
     2461 cgggcgcctc cgcaaatttt ggaaccgcgc tcagcacagt gcaaagcgcc cagcgcgtct
     2521 tgctgcgcgc gcaggacggc aagcccgcaa cattttttgt gggtgaccgc taccccatcg
     2581 atcttggctt gctctctaac agtttgacct ctaactcggc ggcgctcacg caggcgcttc
     2641 tcggcggcgt tacgcttccg cgaaccgatt acgacacggg caatgctccg gtttcagttg
     2701 cgattgcaac gttcaataca aaaaatagcg accaccccga cctggctatc gcaaaccaaa
     2761 cgagcggcac gattacaatt ctagcgggga atggcgacgg cacgtttgta gcgcaagcct
     2821 cgacctgcac gcctccatcg tgcattgctc ttccgtcggt cattacgggc acaaccacaa
     2881 cggctgcgag tccctcttcg attgtcgcgg gtgatttcaa cgatgacggc tttgcggata
     2941 ttgccgtaac cgatcaagcc aacaatcgag tactcgttct gcttggcaat ggggacggca
     3001 cgtttcagac gcccgtcgca tacccaactg ggactaaacc tgtggccctc atcgcgcagg
     3061 attttgacgg ggacagccag gccgacctcg ccatcgtgaa tcaggcggac gggacggtga
     3121 caattcttct gggcaataaa gtgaacggca cgcagaacgg gacgtttggt acgccgccca
     3181 atcagaccgc ggccctcgcg gttggcgctg cgccaagcgc aatcgcaacg ggccagttta
     3241 atatctcgaa taacgcaaac gctgacctcg tggttacgaa tagcaccgac aacaccgttt
     3301 ctgttttgtt aggtaacggc gacgggacat ttggcgcaca acgagtttct aataccggaa
     3361 aagggcctgc gggagtcgcg actgcggatt ttaatggaga cacgaaacct gatttggccg
     3421 tgacaaatca aacggacagc accgtttcaa ttttgttggg gaatggcgac ggaacttttc
     3481 aaacgcaaac tacttttgcc actggatcga gccccgtggg aattgttgcc gcaaatttca
     3541 cgagcgcaaa ttctgatctc gtcgtcgccg atgagaccgc aaacaatgcc gacgtcctga
     3601 ttggcaacgg cgatggaaca tttaacaccc ccatttcatt gcccaccgga aatgctccga
     3661 ttgcactggc aaccgcggac ttgaacggcg actcgactct ggatctcgta accgcgaaca
     3721 atgcttccaa tacggtgacc gtaacgctga acactttgcc gtcttcgacg aatcctgcct
     3781 caagtcaagt tggctatcca tctgcgcagt atgaagatct tgggttaaaa gtgaaggcca
     3841 cgccgcgcct gcacggcgac gatgaagtga ccctgcagct gcaattcgat atcaaaagcc
     3901 tgacgggaac gagcatcaat gggattccca tcttgagcaa tcgaaatatc gagcagacga
     3961 ttcgactccg cgaaaatgaa acttcggttc tgtctggaat tcttcaatcg aacaaaagca
     4021 cgaccagcag cggattaccg tggacttcta ccgctccggg gattggcttg ttgacagggg
     4081 aagatacttc caacaatcag caatccgaat tgctgatcct tgttacgcca agagccttgc
     4141 gcgtgccgcc gcatgaaggt cctgccgtct atgcaggacg cggagaaccg tcttcgccgc
     4201 cgttggggcc gcaattgcct gcgggaatcc cgcagccggg gcagccc
//
LOCUS       DASZMB010000045        10366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3062278, whole genome shotgun sequence.
ACCESSION   DASZMB010000045 DASZMB010000000
VERSION     DASZMB010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 10366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10366
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3062278"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VG272_01935"
     CDS             complement(<1..273)
                     /locus_tag="VG272_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWF12467.1"
                     /translation="MNSDLDLLSPLRRYWGYDAFRPLQERIVRSLITGRDTCVIMPTG
                     GGKSLCYQLPAAMSEDKTVIVISPLIALMEDQVAQLGQMGIPAALLN"
     gene            complement(398..1987)
                     /locus_tag="VG272_01940"
     CDS             complement(398..1987)
                     /locus_tag="VG272_01940"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HWF12468.1"
                     /translation="MRPQNKILALIGLIVFFGSVCMGATISGTVNGVEGAPFQGAFVE
                     AQNAKTKITVVVLSNTQGKYRIPDLAAGDYRVQIKAVGYRAESRNPVTLSHDQNASLD
                     FSLQKGVIRWSEISQYQASQLWPASKGKDLIFGHCNICHQFQSRMASAGRDLDGWKDR
                     VAFMRQAMHFSIFLGPNFSDQDADEVATYLDSLFGPDSVLPKSPAEMPGYQKTVRPFS
                     SDALNIVYVEYEMPAPSRMPFSAAPDKNGFLWIPDFGVANKITRLDPKTGEMKDFSIP
                     FPGTGGIHSAVPAADGSVWLAEQGSNRIGRWDPATEKITEFQDAYLPGKEGIEDGGSK
                     HTVRIDPSGKVWTSGVPLTRFDPETGKFERFVEGVYAYDVKLDKNGDAWFTDPLVNKI
                     GKVDVKTMQVSQWTSPTSNSHPRRMEIGSDGMVYSGEYNGGKMARFDPKSQTFKEFQL
                     PGPDPSPYAMGFDAEGYLWYDSHNMDTINRFDPRTGKVIEYPVPHSELAMREFFRDAQ
                     GRMWYGTAPNNKVGYFYLAGK"
     gene            2224..4014
                     /locus_tag="VG272_01945"
     CDS             2224..4014
                     /locus_tag="VG272_01945"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWF12469.1"
                     /translation="MKKNKRNENNFSRREFIKTAATGAGAAAIAGLGVEAARAEGRPA
                     RWDKEADVVVIGAGATGLPAAIQAAEGGASVILVDANTDVGGHAILSGGNLALGGGTS
                     RQKKYGVVDSPDLLFSDLTDWSVVEPNGFPDYRYNDKEIIRAFADESAKTYEWLVEHG
                     VTFVEKAPDTAGGHACGNSAPRESHSAPMAFPLMQNGGPMPPDKAATTSSGVGLIRPL
                     EASARKLGVQILLQHKMTGIVRENPTSGRVLGISATTLGKTVNIRARKGVIIGSGGHS
                     SNVNFRRIFDPRLTEEYQVAGEPYSFQDGSGEIAAMAIGASLWGAFNQTGEFGQNVTK
                     AGWIGCQYGYVNLTWQPDSPIFAEARASGLSVKDYQDAILVNIAGMRFYDETQGQFPA
                     NNYNSIKSYVPRSYLNAANLKYETSNFINAALAGTGDATPGGGPIWAIFDADAVKREG
                     WKVTPPYVDTKEGYFFSADSIPELAEKIVNKFQKKPMPPNALQETVTRYNSFVDGGKD
                     ADFGKPTPKYKIQTPPFYAAWATPVIHDTRSGLRINAKCQVMDMNGELIPGLYCGGES
                     AGGFSLHGLARCAVQGRIAGRNAATETTKG"
     gene            complement(4031..4321)
                     /locus_tag="VG272_01950"
     CDS             complement(4031..4321)
                     /locus_tag="VG272_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12470.1"
                     /translation="MIGILVMGDNHLIVRGPFPDRQTALALGRHWSLIQIGRATPVEL
                     QRWSISTREFRENLEWAVVVPGDGEVLPGVKQLLDELAARGIRIFDSSLGDW"
     gene            complement(4328..6199)
                     /locus_tag="VG272_01955"
     CDS             complement(4328..6199)
                     /locus_tag="VG272_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HWF12471.1"
                     /translation="MTRFLSRALDDPQCGILNQDTQRLFDYHQATKHTYHSVRTSAHY
                     LDWRNQPNPFRTFEGAPITVLPPEPGFPNVGTFATMGALSERAEAISENTSVGHEPMR
                     LDAVWLSRLLWHSMAVSAWKKVPGSGSRYSLRVNPSSGNLHPTETYLALRGFSDIGDG
                     LYHYRADLHALELRSEGAWTQQLAQTLSIPWAADARLIVGLTAIFWREAWKYRERAYR
                     YCCHDLGHAMMSILVAARALGLPGGAVAHFSDSRMTRTLGLDGGDEAPMIFLVFPQKS
                     FSAPASVLPQETFAGVPNELSSEEVPYKLLLAMHCATILPDPAGPPPHVAAAAIAANV
                     GGPSLQPEILSDPAHDKGLGVVVRRRRSALDFDPRTSPMDRSGAEQLLDFATRDWSAD
                     WHGNFAGDESSPVMKDADFVTLFLYIHRVSECEPGVYRWDGNSRRLQQLHRGNVERVA
                     AFLSLEQALAGNSCFTVSMVADLAKAAQTFGNRGYRYVYFEAGAIGQRLYLGAEALGW
                     NATGIGAFYDDDVHRYLGFLEETDGSVSNSVREAERATLVILDSPTSRHAAAQERNSP
                     EGASAISEQSSNLLSGESEPRANHGKTALPTDSKKLPQQVIYHFAVGRAIPDPRIED"
     gene            6870..7190
                     /locus_tag="VG272_01960"
     CDS             6870..7190
                     /locus_tag="VG272_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12472.1"
                     /translation="MRFQKYSLAVLAILTLTMPVWAKTFKQSVDLSDSKTIGSTQLKP
                     GTYEFTADDSKMELNILQKGKVIATVQGQWVKTSDKSQGLDMQSNKITQIRFHGSDQA
                     FQLP"
     gene            7767..8642
                     /locus_tag="VG272_01965"
     CDS             7767..8642
                     /locus_tag="VG272_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF12473.1"
                     /translation="MKPDCDIPCLRIGRKHLLFCATMVAMTMAVWTSPLSAQQQNSQY
                     SPRFGMGHLPDATVVQNPTVDSKAMAEDAKCFPWKLTEMRAGTVDVQNLSVPSSAKHE
                     YEKACDASKKNKYDEVEQHTRDAIKKFETYPAAWVMLGLSLAEQQKAQDAHDACSHAA
                     AVDAAYLPAYLCSAEVSTRNAEWKQVLTASNTAISLNLGGDSYAFYYRATAYLHMNNL
                     EEAKKNALQAAQIDVNHNEPSLDLLMAQIYEREGDKAQAIAQLQQLLKRRPDRQTAEE
                     ANQRLATLESQQPAK"
     gene            8730..9686
                     /locus_tag="VG272_01970"
     CDS             8730..9686
                     /locus_tag="VG272_01970"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HWF12474.1"
                     /translation="MLSGFSRNLTRQFGWVWVVLLLLPVLSRGQSNSELNLEGAIDIH
                     VHQGPDSMDRAIDADDLARLAKKMGMRGMVMKNHFEETGSLVYMVRKEVPGIELFGGI
                     TQDLAVGGINLEAVKHMVAMKGGWGRVIWLPTFDAENAVKSAKSMAPFVRVSENGHLV
                     PGVLELIDYIATHPDLVLETGHISGEEGDLVVHEAHQRGVAHIVVTHAMASYVRMTIP
                     QMQAAARDGAFIEFVYGATLGTNPVVSISDYAKAIRAIGPKSCILATDFGSVQKPPEP
                     QRVLEPQGLLDFMTALHKEGISVTDVNLMTKTNPALALGLKP"
BASE COUNT         2490 a         2833 c         2710 g         2333 t
ORIGIN      
        1 attgagcagc gccgccggaa ttcccatttg ccccaattgc gcgacttgat cttccatcaa
       61 cgcgatcaat ggggaaatga cgatgacggt tttatcctcg gacatcgcgg cgggcaattg
      121 gtagcaaaga gatttcccgc cgccggttgg catgatgacg cacgtatcgc gccccgtaat
      181 taggctgcgc acgatgcgct cctgcaacgg acgaaatgcg tcgtagcccc agtaacggcg
      241 caggggcgaa agaagatcga gatcggaatt cacacggaga gtatagagct agtcggttat
      301 cgggtccatt ctttgagtga gtagcacggg cttcagactc tggactttag gctttgcaag
      361 gctaaattcc actggctgaa gcctatgctc ctcaacccta cttccctgcc agataaaaat
      421 agcccacttt attatttggc gcggtgccgt accacatgcg cccttgcgcg tctcggaaaa
      481 actcgcgcat tgcgagctcg gaatgcggga ctggatactc gatcactttt ccggttcggg
      541 gatcgaagcg attaatcgta tccatattgt gcgagtcgta ccaaagatag ccctccgcat
      601 cgaagcccat cgcatacggg cttgggtccg gacccggcag ctgaaattcc ttaaatgttt
      661 gagatttcgg atcgaatcgc gccattttgc cgccattata ttctccggaa tagaccatgc
      721 catccgaacc aatttccatg cgccggggat gggaattcga tgttggcgaa gtccattggg
      781 aaacctgcat cgttttcacg tccactttgc cgattttgtt tacgagcggg tcagtaaacc
      841 atgcgtcacc gtttttatcg agcttcacgt catatgcata aactccttca acgaaccgct
      901 cgaatttacc agtttcagga tcgaaccgtg tcagcggcac gccgctcgtc catacttttc
      961 cgcttggatc aatacgaact gtatgcttcg agccgccgtc ctcgatcccc tcttttcccg
     1021 gcaaatatgc atcctggaat tcggtaatct tttccgtcgc cgggtcccat ctgccaatcc
     1081 ggttcgatcc ttgttcagca agccaaacgg aaccatccgc tgcgggcacc gccgaatgaa
     1141 ttccaccggt tccggggaat gggatcgaaa aatctttcat ctcgcccgtt tttggatcga
     1201 gccgcgtaat tttattggcg acgccaaaat caggaatcca caagaaacca ttcttgtcag
     1261 gagcggcgct gaaaggcatg cggctcggcg cgggcatttc atattcgacg tagacgatat
     1321 tcagcgcgtc gctgctgaat gggcgcaccg tcttctgata gccgggcatt tccgcgggag
     1381 attttggcag cacagaatca ggcccaaaca gactgtccag ataagtcgcc acctcgtccg
     1441 catcctgatc gctgaaattc ggtcccagaa aaatgctgaa gtgcatggct tgccgcatga
     1501 atgcaacgcg gtctttccaa ccgtccaaat cccgtccggc ggaagccatg cggctttgaa
     1561 attgatggca gatattgcag tggccgaaaa ttaaatcttt acctttggat gcgggccaga
     1621 gctgggacgc ctggtattgc gaaatttcac tccagcggat cacacctttt tgcaaagaaa
     1681 aatctaagga tgcattttga tcatgcgaaa gcgttacagg gttgcgagat tcggcacggt
     1741 agcccaccgc cttaatttgc acgcgataat cgccggcagc taaatcagga atgcggtact
     1801 tgccttgagt gtttgacagg acgacgaccg taattttggt cttcgcgttt tgcgcttcca
     1861 caaatgcgcc ttgaaacggc gcgccctcca ctccgtttac ggtgccggaa atcgttgcgc
     1921 ccatgcagac agaaccaaag aaaacgatca atccaatcag cgccagaatt ttattttgtg
     1981 gccgcatacg atgctcctaa tgtggtaata ggctgctaaa catgaaataa aattcccttt
     2041 tctcttttaa tatttagaga ctggaccttc cgcaacgtga tagacccaat cctagcgttt
     2101 ttccagattc atggcactac aaatcccggc aactcaaaaa tcgatctgcg gttgacacat
     2161 ttcctcgacg ggcatatcgt cttgcgcaag gcataaatta aataattcac cgggattcca
     2221 tgtatgaaga aaaacaaaag aaacgaaaat aatttctcgc gccgcgagtt tatcaaaacc
     2281 gccgccacag gcgcaggcgc tgccgccatc gctggacttg gcgtggaagc ggcaagggcc
     2341 gaaggccgcc ccgcgcgctg ggataaagaa gcggacgtgg ttgtcattgg tgcgggcgcc
     2401 actggactgc ccgccgcgat tcaagcggcg gagggcggcg cgtccgtaat tttggtcgac
     2461 gcgaatacgg atgtcggtgg gcacgccatt ttgagcggcg gaaatctggc cttgggtggc
     2521 ggcaccagtc gccagaaaaa atacggcgtc gtcgattcgc ccgatctttt gttttcagac
     2581 ctcacggatt ggtccgtcgt cgaaccaaac ggattccccg attaccggta caacgacaag
     2641 gaaattatcc gcgcgtttgc cgacgagagc gccaaaacct atgagtggct ggttgaacac
     2701 ggagttacct tcgtggaaaa agctccggac acggccggcg gtcatgcctg tgggaattcc
     2761 gcgccgcgcg aaagccattc cgcgccgatg gcgtttccgc tgatgcagaa tggcggccct
     2821 atgcctcccg ataaagcggc gacaacttca tcaggcgtag gtctgatccg cccgcttgag
     2881 gccagcgcca ggaagttggg cgtgcaaatt cttctgcagc acaagatgac tggcatcgtg
     2941 cgcgagaatc caacttcagg tagagtgctg ggaatttctg ccaccacact aggaaaaaca
     3001 gtaaacattc gcgcgcgcaa aggcgtgatc atcggctcag gcgggcacag cagcaacgta
     3061 aatttccgcc gcattttcga tccgcgactc accgaagaat atcaagtggc cggcgagcca
     3121 tactctttcc aggatggcag cggagaaatt gcggccatgg ccatcggagc ttccctgtgg
     3181 ggcgcgttta accagaccgg agaatttgga cagaatgtga cgaaggcagg atggatcggt
     3241 tgccagtacg gctacgtgaa tttgacatgg cagccggaca gcccgatttt cgcagaggct
     3301 cgcgcctcgg gactttccgt taaagattat caggacgcga ttctcgtgaa tatcgcgggc
     3361 atgcgttttt acgatgagac gcaaggccaa tttccagcta ataattacaa ttcgattaaa
     3421 tcctatgtcc cgcgcagcta tttgaacgcc gcaaacttga aatatgagac ttccaatttc
     3481 attaacgcag cgctcgcggg tactggggac gcaactccag gcggcgggcc gatctgggcc
     3541 atctttgatg cggacgccgt aaagcgcgaa ggctggaaag ttacgcctcc ctacgtggac
     3601 accaaagaag gatatttttt ctccgccgat tcaatcccgg agcttgcgga aaaaatcgtc
     3661 aataaattcc agaaaaagcc gatgccgccg aatgcgctgc aagagacagt cacacgctac
     3721 aactcattcg tcgatggcgg aaaggacgct gatttcggca agcccacgcc aaaatataaa
     3781 attcagacgc ctcctttcta tgcagcctgg gccacgcccg taattcatga cacacgctcc
     3841 ggcctgcgca tcaacgcgaa gtgccaggtg atggacatga acggtgagtt gattcccggc
     3901 ttatactgcg gcggcgaatc cgccggagga ttcagcctgc atggcttggc aagatgtgcc
     3961 gtgcagggcc gaatcgcggg cagaaacgca gcgacggaaa ctacaaaggg gtaagttcac
     4021 tcgcgaaaat tcaccaatcg ccgagactgg aatcgaaaat cctaattccc ctcgccgcaa
     4081 gctcgtccag caactgcttt acacccggta aaacttcgcc gtcgcccggg acgactacgg
     4141 cccattccag gttttcccgg aattcccgcg tgctgatgct ccatctctgc aattcgaccg
     4201 gagtcgcccg cccaatttgt atgagggacc agtggcggcc gagggccaag gccgtttgtc
     4261 gatccggaaa cggcccgcgc acaatcaagt ggttgtcgcc catcactaga atcccgatca
     4321 tgccgattta atcctcaatc cgcggatcag gaatagcgcg gccaactgcg aagtgataga
     4381 tcacctgttg cggaagcttt ttcgaatccg tgggcagagc agttttgcca tgatttgcgc
     4441 gtggttcgct ttctccggaa agcagattgc tactctgttc ggagattgcc gaagcacctt
     4501 caggagaatt cctttcttgc gccgcggcgt ggcggctcgt cggcgaatcc agtatcacga
     4561 gcgtcgcgcg ttccgcttca cggacggaat tgctcaccga tccgtcggtt tcctccaaga
     4621 atcccaggta gcggtgcacg tcgtcatcgt aaaaagcgcc gatacctgta gcattccagc
     4681 ccagagcctc cgcgcccaaa tataatctct gcccgatggc gccggcttcg aaatacacgt
     4741 agcggtaacc tcgattgcca aatgtttgcg ccgccttcgc caagtccgcg accatcgaaa
     4801 ccgtgaagca cgaattcccc gccagcgcct gctcgaggct gagaaaagcc gccacgcgct
     4861 caacatttcc gcggtggagt tgctgcagcc tccgactgtt gccgtcccag cgatagacac
     4921 cgggctcgca ctcacttacg cggtgaatat aaaggaataa ggtgacgaag tcggcgtctt
     4981 tcatgactgg cgaactttcg tcaccagcaa aatttccatg ccagtctgcg gaccaatctc
     5041 gcgtcgcaaa atccagaagt tgttccgcgc cgctgcggtc catgggcgaa gttcgcggat
     5101 cgaagtcgag tgcggaacgc cggcgtcgca cgacgactcc aagaccttta tcgtgcgcag
     5161 gatcactcaa aatttcaggc tgcagcgacg gaccgccaac attggctgca attgctgctg
     5221 ctgcgacgtg cggcggcggc cccgcaggat cgggcaggat cgtcgcgcaa tgcatggcga
     5281 gcagcaactt gtatggcact tcttccgagg acagctcatt cgggactccc gcgaatgttt
     5341 cttgtggcag cacagacgct ggcgcggaaa aacttttttg cggaaaaacc agaaaaatca
     5401 tcggcgcttc atcgcctccg tctaagccaa gcgtgcgcgt catacgcgaa tctgagaagt
     5461 gagcaaccgc gccaccaggc aatccgagcg cgcgagccgc cacaagaatg ctcatcatcg
     5521 cgtgtcccaa atcatggcag cagtagcgat atgcgcgctc gcgatacttc cacgcctcgc
     5581 gccagaaaat cgcagtaagt cccacaatca atctcgcgtc agcagcccac gggatcgaga
     5641 gtgtctgtgc taattgctgc gtccacgcgc cctcgctgcg aagctcgagc gcatgtaggt
     5701 ccgcgcgata gtgatacaaa ccatcgccaa tgtccgaaaa cccgcgcagc gccagataag
     5761 tttccgtagg gtgcaaattt cccgaagatg gattgacgcg caggctatag cggctgcccg
     5821 agcccggaac ttttttccac gcgctcaccg ccatcgaatg ccaaagcaaa cggctgagcc
     5881 agaccgcatc caaccgcata ggttcgtggc ccacggaagt attttcactt atcgcttccg
     5941 ctctctcgct aagcgccccc atggttgcaa atgttcccac gttcggaaaa cccggctcgg
     6001 gcggaagcac agtgatcggc gcgccttcaa aagtgcggaa cggattgggc tgattgcgcc
     6061 aatcgagata atgggcactc gtgcgaacgg aatgataggt atgcttcgta gcctgatgat
     6121 agtcgaagag gcgctgcgta tcttggttca ggatcccaca ttgaggatcg tccaacgcgc
     6181 gagagagaaa gcgcgtcatg ctgatatccg ctgaaaactt gcgatgcgtt tcaattcgct
     6241 tctcgctccc gttcacacgt acgaatcaca cacgcaaaca aaacaccggc gccagttttc
     6301 gatcatagca ctctgcttcc ctgttgaaag aattggagga ttggtagccc ctcgactttc
     6361 ggggcaggcg ccctctccgt cccaaaaaat acggttcacc gagaacgttc tccgtgaatg
     6421 ggacggaaat gcctctacaa aaccgccttg gctttgcttt tgtagcggcc gcccatcttt
     6481 tcgggcgggc agcttttgtg cttctagaaa tttggctcgc aaagctaaat caatcaacgt
     6541 tcgcctttaa ggatttcgcg agcgagcgaa tcaaagattg acctacttgc gtgaggtata
     6601 taatcgcgca cttgagccta tcgaagaagt ccgttcccgc cgcgcggatt caacaatcgt
     6661 ttaaataagc cgcccgcagt gcgtgcatat tgcacgacgc aaaaaattcc tcagtcttca
     6721 tgtgaacttc aggttgacca tatagaatct gcttgcgtgg cactgggata gatcttcaac
     6781 cggcgccgcc aagctaaccc ttcggaattc catcgatctt tgcgattttc caaggggaat
     6841 tcattctaat cattcggagg aaggtataaa tgcgctttca gaagtattcc ctcgcagttt
     6901 tggcaatctt gacactcacg atgcccgttt gggccaagac ctttaagcaa tctgttgacc
     6961 tcagtgatag caaaacgatc ggaagcaccc aactcaagcc agggacatac gaattcacgg
     7021 ccgatgattc caagatggaa ctcaatatct tgcagaaggg aaaagtcatt gcgacagtcc
     7081 aaggtcagtg ggtcaagact tcggataagt cgcaaggact cgatatgcaa agcaacaaga
     7141 tcactcagat ccgattccac ggaagcgatc aagcgtttca actaccataa gaactccgca
     7201 aggacgagtt gcaagtgttt cttattcgct tctggtgctc ttcggggttt cgagccaggc
     7261 cctgataacg gccttctggg aaattgggat ttagatattg ccgatttccg acaagagcgt
     7321 atatcagggc gtggcacgcg ttggcttaac cctatgtgtt gttgctctac gctacgcagt
     7381 ggtggctgtc cgcgcacagg tgacccactc gccagttgag gctaccgacc agcgattatt
     7441 tccctttttc aaagctatat acttccctgc tcactctaac tttgatttat acaatcagac
     7501 tttccgctgt atctgttcaa gttgtgttcg tgttgtgttc gtgccactcg tacagtccga
     7561 acggtccttg ggcaattgtg ttcggtctga tctagccatg gtgtctcacg ccagcaaggg
     7621 cattagttcc taaaatgtcc tggcctcagg cgaatccgtt tgactcaatt tggaatatcg
     7681 gcataggatt cgaaataaga aacctgcctg cgaacggccc agggtgctcg atttcaactt
     7741 cgcgacgcgg cagcggaagg tacacgatga aaccggattg cgacattcct tgcctgcgca
     7801 ttggtaggaa gcacctgcta ttctgcgcga cgatggtggc gatgacgatg gcagtgtgga
     7861 cttcgcccct ttctgcgcaa caacaaaaca gccaatattc tccaagattc gggatggggc
     7921 acctgcccga cgcgaccgtc gtccagaacc cgaccgtgga ttcgaaggcg atggccgaag
     7981 atgcaaagtg ctttccctgg aagcttacgg aaatgcgggc tggaaccgtg gatgttcaga
     8041 atctgagcgt tccgtcgagc gcgaagcacg agtatgaaaa agcatgcgat gcttcaaaga
     8101 agaataagta cgacgaagtg gagcagcata cgcgcgatgc gatcaagaag tttgaaacct
     8161 atccggcagc atgggtgatg ttaggcctga gtctcgcgga acagcaaaaa gcgcaagacg
     8221 ctcatgacgc ctgttctcat gccgctgcag tcgatgcggc atacttgccg gcgtatctat
     8281 gctcggcgga ggtttccacg cgaaacgctg agtggaaaca agtcctgacc gcgtcaaaca
     8341 ctgcgatcag tctcaacctc ggaggcgact cctacgcctt ttattaccgg gccacagcct
     8401 acctccatat gaataatctc gaggaggcca agaaaaacgc tctccaggcc gcgcagattg
     8461 acgtaaatca caacgagccg tctcttgatt tgctcatggc gcaaatttat gagcgcgaag
     8521 gcgacaaagc gcaggcgatc gcccaactcc agcaactcct gaaacgccgt cccgatcggc
     8581 aaaccgcaga ggaagctaac cagcgcctgg caacgctcga atcgcagcaa cccgccaaat
     8641 aggcctgcca catgcgtttt gccgctcacg attcaaagaa tcaagccgtc gtatctcgcc
     8701 acagtccgcg ccgccgcaag gagtatcgga tgctttcagg attctcacgc aatctcaccc
     8761 ggcagtttgg ttgggtttgg gtcgttttat tgctcttgcc tgtgttgtcg cgtggacaat
     8821 caaattccga attgaattta gaaggtgcga ttgatatcca tgtgcatcaa ggtccagaca
     8881 gcatggaccg ggcgatcgac gccgacgatt tggcgcggct tgccaagaag atgggcatgc
     8941 gcggcatggt aatgaaaaat cactttgaag aaaccggctc gctggtctac atggtccgca
     9001 aggaggttcc gggcatcgag ctttttggag gaattacgca ggatctggcc gtcggcggaa
     9061 taaatctgga agcagtgaaa cacatggtcg ccatgaaggg aggctggggc agagtcattt
     9121 ggctgcctac gttcgatgcc gaaaacgctg tgaagtccgc aaaatcaatg gctccgtttg
     9181 ttcgcgtttc tgagaacgga catctggttc ctggtgtgct ggagctgatt gattacattg
     9241 ccacgcatcc ggacctggta ctggagaccg ggcatatctc aggggaagaa ggagacctcg
     9301 tggttcatga ggcccaccag cgcggcgttg cgcacattgt ggtcactcac gcgatggcgt
     9361 cctacgttcg aatgaccatc ccgcaaatgc aagcggctgc ccgggacgga gcatttatcg
     9421 aatttgtgta tggcgcaacc cttggaacga atccggttgt cagcatcagt gattatgcca
     9481 aggccatccg cgccatcggc ccaaaatcct gcattctggc cacggatttc ggctccgttc
     9541 aaaaaccacc ggagccgcag cgtgtgctcg aacctcaagg tttgctggat tttatgacgg
     9601 ccttgcataa agaaggaatc tccgtgacag atgttaacct catgaccaaa accaaccctg
     9661 cactcgcatt aggtctcaag ccttaaccga attccaccgc acaaaaataa cgccggagaa
     9721 acaagatttt cggtaactgg catttcgcag cttggcctca tacaccggag atggtttgtc
     9781 gggcatcggc acggcttcta aagtcgaatg gaaaagccag tgccatttca gttgttactc
     9841 tctcggacat cttgcaaatg ataagttgaa atacacagcc tcagactgtg ctaacttact
     9901 ttacggtagc ttatatctcc ccctctcggg ttattgccgt tggtagcatt gtatcgatgt
     9961 gtttaagcgg tttcctgata ttcccttccg acaaaagcgt tgcatcgcaa tttaacctgg
    10021 taattataag catttagtcc agatggcact tttttgatag tcggtgcata cggaagctct
    10081 attgcagtca acgactcaaa acttacagaa ggcggaccaa ctctgaaatt aaagaggtac
    10141 tccgcgtttc aaaaggctgt gttcgaatat gaatgaaacg atgctcctcg aaagttccca
    10201 agtgaaacga aatctgggca gatccattat tactccgagc actaaatatg tgacacgttt
    10261 cagcgagctg cgtactgcac gtggcgctcg cgaaagtagt tcttcaccgc cttgggtctg
    10321 gcctgtgtga tccgcaggta agagcgaacg ttgtccatca gttcct
//
LOCUS       DASZMB010000046        17482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4175347, whole genome shotgun sequence.
ACCESSION   DASZMB010000046 DASZMB010000000
VERSION     DASZMB010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 17482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17482
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4175347"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VG272_01975"
     CDS             <1..470
                     /locus_tag="VG272_01975"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HWF12475.1"
                     /translation="GNKDETIELHPGMASADVEKTFAAMLETVPYASGVNNHEGSLGT
                     ADQGLMDELMPLLHQRNLFFIDSRTTTATVAESAAHVAGVSTARRNVFLDDEQSVVAT
                     RKQFALAIRDAKEKGSALAIGHPHATTLEVLNEMLPEAERQGVRLVFASDLAR"
     gene            complement(524..2305)
                     /locus_tag="VG272_01980"
     CDS             complement(524..2305)
                     /locus_tag="VG272_01980"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HWF12476.1"
                     /translation="MNDRKELAEETAVSSPEANSKSRVTNGLDFKWLSPLARSFKLSE
                     GNLFLLLAIIIGIFSGLAVVCFRIAIEWVRLALLGSALSPPHIRVLLVPAGAGLVVAF
                     LVQKFFLAARGSGVNQTKAAVYVFDGYVPFRTVIGKFLTCSIAIGGGQSLGPEDPSLQ
                     MGAGIASALGRRMKLSRDKLRLIAPVGAAAGLAAAFNAPISAVLFVIEEVIGTWSASA
                     LGAIVLAAVSSAVVMRSFLGGEPMFRVPAYTLAHPAELLAYAVLGIVSGVLSLAFTRI
                     IVIFRPRLLAMPEWTHYLQPAAAGLLIGFVGLKIPQAMGTGYPYIDQALHSEYTWQIL
                     AILGFAKILTTSLSFVTGTPGGMFAPTLFIGAMIGGAIGGLEHQFFPQVSASVAPFAL
                     VGMGTFFAGFLRVPITSVFMVVETTGSYSIVLPVMISNTIAYLISRKYQDVALFDLLA
                     KQDNMELPSMEEQREQIVLRVEDAFRQPNLPPLQASDTLARAMEVAEVSTEDCLLVRF
                     PTGRWAAIARKDLPEIAAKYPKETQLREVLSTARLPILHPDQRLDDALRFIQGHELLP
                     VIGRAGSRKLEGVISLPDILAAYQKAS"
     gene            complement(2302..3402)
                     /locus_tag="VG272_01985"
     CDS             complement(2302..3402)
                     /locus_tag="VG272_01985"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF12477.1"
                     /translation="MSLILLALLFVVTAALARAYHAREGALAKEWSDRGNRDLSASKP
                     AKAFEDYRNSLSYAPENPDVQLDLAEALLADGRLTEARSYLLNLWDHAPGSGEVNLDL
                     AHLSMQMGDADQTIQYFRGAILGSWEGESASHRRKARIELSEFLLDKRRMDEAQAVVA
                     GIAADTPAEDGSVHEKNGHLFLRVGDPARALAEFEAALQTDPHNSQWLAEAAQVAFED
                     GNYSKAETYFSRAAREDSTDNLRASLDLVRDILRNDPFLAGLSEDEQTRRTWRDFQQG
                     MDRLRKCTRAGASSSASSHSSSDLDALNKQAQELQKRVNLSSLGRDSDLRNEAMQLVS
                     GIEDVASQICGPPTGIDQALKLLKVQQEGINQ"
     gene            complement(3765..4052)
                     /locus_tag="VG272_01990"
     CDS             complement(3765..4052)
                     /locus_tag="VG272_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HWF12478.1"
                     /translation="MAKPLSKSKIVAHMADKVGTSKKVAAQFFEELFKLAVKEAKGGA
                     GKFVIPSIGRVVKAHRKARMGRNPQTGEAIKIKAKTVVRLRAAKALKDAIL"
     gene            4263..4973
                     /locus_tag="VG272_01995"
     CDS             4263..4973
                     /locus_tag="VG272_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HWF12479.1"
                     /translation="MTNWNEFEEQFTDCLALDRRPVAVTFLDSIPSNVPKFSGTEPAG
                     CSYWRLAAAGRTFYTVPADHFNCAVGAYTHNVQLPPDRVQETEATLGMMFNIGYVRPE
                     EVPGIPRLAKEPVAVVFAPLGDTPVDPSVVLFSCKTSAAMLLNEASLRAGASSSLPLL
                     GRPSCMALPAALAYGTVTTLGCIGNRVYTDLSADELYIAVPGAKISAVSDALGIISTA
                     NATLEEYARGRQAALSTV"
     gene            complement(5062..6429)
                     /locus_tag="VG272_02000"
     CDS             complement(5062..6429)
                     /locus_tag="VG272_02000"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HWF12480.1"
                     /translation="MNLTDYIDSKHDEHLNELYEFLRIPSVSAQSEHKPDIERAAKWV
                     AEKLRAAGLDNVEILPTKMHPLVYGESLRAPGKPTILFYGHYDVQPAEPLDLWTSPAF
                     EPTIRGGNLFARGSADDKGQVHIHLRALDAIMKTAGKLPINLKVMIEGEEEVGSVSLW
                     DFVSANQARLKADALVVSDTGMLAPGVPSITYALRGLNYYQIEVTGPAQDLHSGIFGG
                     AVPNPITILCELFAKLHDKNSRVAIPGFYDDVANLSKAERKGLKSLPWKASEFKKTVG
                     APGLTGEKGFSIIEQLGARPTLELNGIWGGYTGEGAKTVIPSKAYAKFSTRLVPNQDA
                     DKITRLVKRHIAKLVPKTVKFKIDVHSQGNPWAAPYTHPFFEVAHNALEKGFGKRAVF
                     IREGGSIPFVTQMHDTFKVPCILLGFGLPDENAHAPDEHIALENYFGGIKAVAQFYEG
                     LGNLK"
     gene            6623..6985
                     /locus_tag="VG272_02005"
     CDS             6623..6985
                     /locus_tag="VG272_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12481.1"
                     /translation="MPEKDLDNARRSTRVFMRVRVVVAGKNSDNRRFREACETIVINA
                     HGGLLYLNQPLDMDAMLVVTNPFTQEEQESRIVFIGDATNKGQRVGIEFLTPAPHFWG
                     VDFVPMDWPAPPATVRSR"
     gene            complement(7019..7522)
                     /locus_tag="VG272_02010"
     CDS             complement(7019..7522)
                     /locus_tag="VG272_02010"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HWF12482.1"
                     /translation="MSYVEKHLIDGESIVYETRLHWIVLVAPVVLGILFALTGVGMFI
                     LSSRATGDRSSAHEPTMVLGGVFFALALFFVVRGIVMRNSTEMTVTNKRVFVKVGLAA
                     RRTIELLLSRVESIGVQESAMGRVLGYGAVVIHGTGGTPETFNLVSHPLEFRTQVQMQ
                     IEKLPSK"
     gene            7811..9310
                     /locus_tag="VG272_02015"
     CDS             7811..9310
                     /locus_tag="VG272_02015"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HWF12483.1"
                     /translation="MNSKQSALSPKTKTGANLTRRGILRGAAGAVAAASLSRISLFAE
                     DSVSPPMARLSSYMSEARNHALPDDVTEKAKHHILDTFAAMVSGSELVPGQAALKFAR
                     NYGGEKIATVICSNILCGPMEAALVNGVLGHSDETDDAHAPSGSHPGVAIVPAALAVG
                     EKFGISGAEFLRAITLGYDVGTRFVMTMGGAAIQSEQHRSTHSIAGIFGAAAAAASAA
                     SLNEQQMRWVLDYTSQQSSGTAAWQRDTQHIEKGFVYAGMPARGGVTSALLVQSGWTG
                     VDDVLSGADNFFMAYAPQSNPASLVDKLGERFAVTETDIKKWTVGAPIQAVLDAVDIL
                     IKRRSFSADDVKEVVVRLARTQGTVVNNREMPDICVQHMVAVMLIDKSASFKSAHDKA
                     RMQDPAVLRVRAKVNLVLDDGELQRALPRREAIVEITLNDGTRLNEHVKTVRGGAANA
                     MTREEVVAKARELMVPVLGAANGSALIEKMLNLENVKSVQELRPLLQLV"
     gene            complement(9448..11205)
                     /locus_tag="VG272_02020"
     CDS             complement(9448..11205)
                     /locus_tag="VG272_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWF12484.1"
                     /translation="MSSRPDAQSKTEIRDGMKIDWDAPISMDDGIVLRADVFRPVNEG
                     RFPVLLTYGPYAKGLSFQDGYPDAWETMAREHPDVPAGSTNLYQNWEVVDPEKWVPHG
                     YVCVRVDSRGCGRSPGYVDPFSPRETKDFVACIAWAGEQPWSTGKVGLNGISYYAINQ
                     WQVASQQPPHLAAMCVWEGAAEWYRDTSHHGGIYCTFWANWYDMQVKTVQHGLGTRGL
                     CSRATGDLVSGPETLTEAELAANRCNFGADLFAHPLDDQYSKDRSPDWSKVTVPFLSA
                     ANWGGQGLHPRGNFEGFMRAASTHKWLESHGIEHWTHFYTDYGRDLQKRFFDYFLKGE
                     KNGWDQQPQVLLQVRHLDRFEERVENEWPLARTKWTKFYFDFANGGLAAVPLTADPAS
                     VSFDAMGDGVTLMTPPLTAETEITGPSALKLFLSSTTEDADIFAVLRVFSPDMKEVVF
                     QGAIDPHTPIGQGWLRASNRKLDAAISKPWRPYHTHDERQPLKPGIAVEMDVEIWPTS
                     MVVPVGYRIALTIRGKDYEHACRGGKLSNFKNELRGCGPFLHDDPTDRPANIFSGKTT
                     LHTSKEQRPYLLLPITPKK"
     gene            complement(11234..12439)
                     /locus_tag="VG272_02025"
     CDS             complement(11234..12439)
                     /locus_tag="VG272_02025"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF12485.1"
                     /translation="MDSFIYALVLVPALRDLLPRSGIPATTANVGYYGGILFALFLVG
                     WGLALLWGPVADRFGRVRALMLTIICFSLFTLLSAFATNIWMLATLRLLAGIGIGGEW
                     GIGATLISEEWPEDRRTMGAGMMHTGYYFGFFLAALANYFVGSRYGWRWMFVVGGVPA
                     LLVILIRRGMKEPARWESKRGKLGERWSLQRSFMEIFSVRYRLRTVMNLMYQLVSTIG
                     LWAGSVYVPAAITYLAAMSGHSPFQSARLASYGTGILSIGTILGALSVPAIADRFGRK
                     INQAIFYSLMFVVIVITFGYVFYLQTDTLKWFFVCLFFLGIGGANYTVYSFWLPEQYE
                     TECRASALAFAQNVGRFAGAGATFLVGVGVRHFQTLGIPVAMTAAAFLVGLLLLPFGM
                     ETKGKPLPS"
     gene            12888..14372
                     /locus_tag="VG272_02030"
     CDS             12888..14372
                     /locus_tag="VG272_02030"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HWF12486.1"
                     /translation="MKRSQKANLPIGKSEKNLTRRDLVKHGIWVLAATAIPGAVKLAA
                     EDISPMMLKLSTYMSEARNNALPDKVLQETKHHILDTFAAMISGSELPPGRMALKFAH
                     AYGGPSISTVVASDILMGPIEAAMVNGELAHSDETDDDYTGGGAHPGSAVIPATLAVG
                     EKIGITGQHFTRAVALGYDIGMRAFKTVGAGILKDTHVLVGTFGASAAAGCVLSLDAQ
                     QMRWLLDYAAQQAGAGFATWRRDTDHIEKGFVFGGSTARNGVTGALVVQLGWTAVNDV
                     MSGPDNFVMSYDPKADPAKFVEGLGERYEITLTTLKRWTTGGPIQSPLDALQLIMKKH
                     PFEPDQVKQVVVRTATSAAYTVNNREMPDICLQHMIAVMIIDKTASFKAAHDKARMQD
                     PAILRVRAKVQLIGDEEFERLIPKRVAVVEVTLNDGTKLSERVESVRGTPENPMDREE
                     VVSKARDLIIPILGLKKCDTLVDKLLNLENVKDIRELRPLLQRT"
     gene            14503..14694
                     /locus_tag="VG272_02035"
     CDS             14503..14694
                     /locus_tag="VG272_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12487.1"
                     /translation="MKIIVRILSAIFVLMGTIWFLQGINVLPGSFMTGQLRWAVRGGI
                     LFVVGVAGLLWAKRDPKHV"
     gene            complement(14799..15725)
                     /locus_tag="VG272_02040"
     CDS             complement(14799..15725)
                     /locus_tag="VG272_02040"
                     /inference="COORDINATES: protein motif:HMM:NF023165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HWF12488.1"
                     /translation="MRIIKFALIMMLLVLAAVPAFAQGPPSFAPPQLEQLVSRIALYP
                     DPLVAQILAGATYPDQIPDAARWADQHHYLTGQALADAIQGDQLPWDPSVQALLPFPS
                     VLEMMASDMNWTTDLGNAFLAQQGDVMDAVQRDRRKARDYGYLRSNGQVVVGGGPNIT
                     IMPVNPGFVVVPSYDPRVVFYAPRPGFFVGGAIRFGFGVSLGLSFRPWGWGYNRFDWG
                     GHGVFINNARWGRTWYNRGEYAHPYAGVHRYGPGERAPEHHELEQRSERERGAEREGR
                     RAPEEHRGHGNDHGGDHGHDHGGDHGGDHDRR"
     gene            15964..16518
                     /locus_tag="VG272_02045"
     CDS             15964..16518
                     /locus_tag="VG272_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWF12489.1"
                     /translation="MALPVKNEGFNTPYEVDPAVRIGHVHLKVADLKRAVAFYSGVLG
                     LEVMQKLGEEAVFLSAGGYHHHIALNTWESLGGSPPPVGATGLYHTAIVYPTRAALAT
                     ALRRVLAARISLEGAADHGVSESIYLRDPDDNGVELYCDKPKELWPRDAKGDLAMYTR
                     MLDLNKLLSEAPSGSAVSTGPEDL"
     gene            16705..>17482
                     /locus_tag="VG272_02050"
     CDS             16705..>17482
                     /locus_tag="VG272_02050"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HWF12490.1"
                     /translation="MIRRDFCKTSLALAGLALGKGPAEALASSAVASPAEELAKAPGL
                     TKYVSEFIVNTKYEDIPENVITIGKKTMLDGFGLALAGSASTAGPRIRQYIESLGPCG
                     GSASIIGTKMKVHPRFAALANGISIHADDYDDTGSAFHVASPVLPPSFALCEVGKRSG
                     QDLMLAFHVGVEVGNKIGDAMSPRHQQDGYHTTGTIGTMGSAASCAKLRGLDTAKTAI
                     ALAIAGSEASGLRDNFGSMTKPFHAGHAGENGTVAVDLAAI"
BASE COUNT         4082 a         4831 c         4699 g         3870 t
ORIGIN      
        1 cagggaacaa ggacgaaacg attgaactgc atcccggcat ggcttccgca gacgtggaga
       61 aaacttttgc agcgatgctg gaaacagttc cctatgcatc aggtgtgaac aatcacgaag
      121 gctcgctggg caccgccgat caaggactca tggatgagtt aatgcccttg cttcatcagc
      181 gcaatttatt ttttatcgat agccgcacaa ctactgcgac tgttgcggaa tcggccgcac
      241 atgtggcggg cgtgtcaacc gccaggcgca atgtgttttt ggacgacgaa caatcggttg
      301 tggctacacg caagcaattt gcgctggcca ttcgagatgc caaggaaaaa ggttctgccc
      361 tggcgatcgg gcatccgcat gccaccacgc tggaagtgct gaacgaaatg ttaccagagg
      421 cggagcggca gggtgtgcgc ctggtatttg cttctgattt ggcgcggtag ttttgccgtg
      481 cctattttag cctgtcctat ttttctatta gtcgaatgag aaactaagaa gctttttggt
      541 aggcggccag aatatccgga agagaaatca cgccctctaa ttttcgcgag ccggcgcgac
      601 cgatgacggg caatagttcg tggccctgaa taaatcgcaa cgcatcgtcc aatcgttgat
      661 cgggatgcag gataggcaag cgggcggtcg acagaacttc gcgcagctgc gtttctttcg
      721 gatattttgc ggcaatctcc ggcaaatctt ttcgtgctat tgcggcccag cggccggtgg
      781 gaaatcgcac cagcaggcag tcctcggttg aaacttccgc aacttccatg gcgcgagcga
      841 gggtgtcgct ggcttgcaat ggcggtaaat tcggctggcg aaatgcgtcc tccacgcgca
      901 gaacaatttg ctcgcgttgc tcctccatcg acggcaattc catgttgtcc tgtttggcaa
      961 gaagatcgaa tagtgcaaca tcctggtact tccgggaaat taagtaagcg attgtgttgg
     1021 agatcataac cggcagaaca atcgaatagc ttcctgttgt ctcaacaacc atgaagaccg
     1081 acgtgatggg aacgcgcaag aagcccgcaa aaaatgtgcc catcccaacc agggcaaatg
     1141 gagcgacgga tgcgctcact tgagggaaaa attgatgttc gagccccccg atggccccac
     1201 caatcatcgc gccgataaat aatgtgggcg caaacattcc tccaggagtt cctgtgacga
     1261 aggataaact cgtggtcaat atcttcgcga atccaaggat ggccaaaatc tgccaggtgt
     1321 actcgctgtg aagagcctgg tcgatatagg gatatcccgt tcccatggct tgcggaatct
     1381 tgagtcctac gaacccaatc aaaagtcccg cggcggctgg ttgcaggtaa tgcgtccact
     1441 ccggcatggc gagcagcctt ggcctgaaaa ttacaatgat tcgtgtgaaa gcaagcgaca
     1501 gtacgccgct gacgatccct aatacggcgt atgccagaag ttcagcgggg tgtgccagcg
     1561 tgtacgctgg aacgcggaac ataggttcgc cgccgagaaa agaacgcatg acgacggcgc
     1621 tggagactgc ggccagtacg atggctccca gagcgctggc actccacgtg ccgatcacct
     1681 cttcaatcac gaacaaaaca gcggaaattg gggcgttaaa cgcagccgca aggcctgcag
     1741 ccgcgcccac cggcgctatg agcctcagct tgtccctcga aagtttcatg cgtctgccga
     1801 gcgcggaggc tatccctgcg cccatttgca gcgacggatc ttcggggcca agcgattgac
     1861 cgccgccaat tgcaatcgag caggttagaa attttccgat gacggttcgg aaaggcacat
     1921 agccatcgaa tacatatacg gccgccttgg tttgatttac tccactgccg cgagccgcaa
     1981 ggaaaaattt ctggaccaga aaagcgacca ccaacccagc tcccgccggg actaataaca
     2041 ctcggatatg cggcggagaa agcgcggacc caagaagggc gagacgaacc cattcaatag
     2101 caattcggaa acaaaccaca gccaagcctg aaaaaattcc aataatgatc gccaacagca
     2161 ggaataaatt cccttcagac agtttgaagg atcgcgcgag aggacttaac cacttaaaat
     2221 ccaagccatt tgttactcga gattttgaat ttgcctcggg agaagacacg gccgtttctt
     2281 cagccagctc ttttctgtcg ttcattggtt aatgccttcc tgctgcactt ttaatagttt
     2341 taaggcttgg tcgatgcctg tcgggggtcc gcagatttgc gaagcaacgt cttcgattcc
     2401 ggaaacaagc tgcatcgctt cgttgcgcaa atcagaatcc ctgcccagcg aagacagatt
     2461 tacgcgcttt tgtagttctt gcgcctgctt gtttagagca tccagatcgg aggaagaatg
     2521 gctcgaggct gagctgcttg cgcccgcacg tgtgcatttg cgcagccgat ccatcccctg
     2581 ttggaaatcg cgccaagtac gacgtgtctg ttcgtcttcg ctcagcccag ccagaaaagg
     2641 atcatttcgt aaaatgtccc gaaccagatc gagcgacgcg cgaagattat ccgtggaatc
     2701 ttcacgagca gctctggaaa aataagtttc cgcctttgaa taattcccat cctcaaatgc
     2761 aacttgcgcg gcttcggcta gccattggct attatgtgga tcggtttgca aggcggcttc
     2821 aaattcggcg agagcccgag ctggatctcc cacgcgtaag aacagatggc cgtttttctc
     2881 atggaccgat ccatcttcgg ctggtgtatc cgcggcgatt ccggcgacca ccgcctgagc
     2941 ctcgtccatc cttcttttat ccagaagaaa ctcagagagt tcgatccgtg ctttccgcct
     3001 gtgagaagct gattcccctt cccagctgcc caaaattgca ccgcgaaaat attggatcgt
     3061 ctgatccgca tcacccatct gcatggaaag atgagccagg tccaggttca cctcgcccga
     3121 tcccggagca tggtcccaaa gattcagaag gtatgacctt gcttccgtaa gccggccatc
     3181 ggcaagcagt gcctctgcca agtcgagctg cacatcgggg ttctccggtg cgtaagaaag
     3241 ggaattacga taatcctcaa aagccttcgc gggcttactg gcagataaat ccctgtttcc
     3301 cctgtcggac cattccttgg caagcgctcc ttcgcgtgca tgataggcgc gggcgagcgc
     3361 cgcggtgaca acgaatagta gcgcgagcag aatcaggctc atcaacagaa cccgctgttg
     3421 taaatatcgc gatacgatat gttgggattc agacgtcaca atttaaagat aacccagagg
     3481 agatactgaa tacaccaatt tgtaatggaa gacgaataca gattttaccg ggactcccaa
     3541 taaatgcctg aacgcctacc aattgtccgg aaaaaataat gccccggagg gctgggcccg
     3601 gggcggtaat gaaacaagag tgacgacagg cagcttggaa gcgatgctgg gtgaatctgc
     3661 ggtgtttacc cattctggcc acttcagaga ggggaccaga accaccttga tacacccagc
     3721 ccgcacgtag cttcgaatcg tctggttcgc gaatctcagt ctagctagag aatcgcgtct
     3781 tttagagcct ttgctgcgcg aagacgaaca acggtttttg ccttgatctt gattgcttcg
     3841 cctgtttgcg ggttacggcc catgcgcgct ttgcgatgtg ccttcacgac gcggccaatg
     3901 ctcggaatga cgaacttgcc tgcgccgccc tttgcttcct tgaccgccag cttgaacagt
     3961 tcttcgaaga actgcgctgc aaccttcttg gatgtgccga ctttgtcagc catgtgcgcg
     4021 acgatcttgg acttgctcag tggttttgcc atctagtttc cttctcctcc tcagaattga
     4081 gactacacat gtgcggccct gttcgaaatg caagagccac gcacggcgag cactatacgg
     4141 gcgaagcgcg ctcaacgcaa ggaaaaacgt ataaaaagcc agattttgcg cggcttccag
     4201 caatgctact cttggaatat gaatgtgcta ttctttttgc tcctacgtta caaggggtgt
     4261 aaatgaccaa ctggaatgaa tttgaagagc aattcactga ctgcctggcc cttgaccggc
     4321 gtcctgtcgc tgtcacgttt cttgacagca ttccatcaaa tgtcccgaaa ttctcgggta
     4381 ctgagcccgc cggttgcagc tactggcgtt tggccgctgc cgggcgcacg ttctacacgg
     4441 ttccggcgga tcactttaat tgcgctgtgg gagcctacac gcacaatgta cagcttcctc
     4501 ctgaccgcgt tcaggaaacc gaagcaactc tgggcatgat gtttaatatt ggctacgtgc
     4561 gcccggaaga agttcccggc attcctcgac tcgcaaaaga gcctgtcgct gtcgttttcg
     4621 cgccgctcgg tgacacgcct gtcgatccta gcgttgtact tttttcatgc aaaacttccg
     4681 cagccatgct gctgaatgaa gcttctctcc gcgccggcgc cagcagttct ctcccattgc
     4741 tcggtcggcc atcgtgcatg gcgctccctg cggcccttgc ttatggaaca gttacaacat
     4801 tggggtgcat cgggaatcgc gtttacacgg atctaagcgc cgatgaactt tacatcgcgg
     4861 taccgggcgc taagatttcc gcggtgtccg atgccctggg aatcatcagc acggcgaatg
     4921 caacgcttga ggaatatgct cgtgggcggc aggcagcact gtcgacagtt taattttcgt
     4981 tttaagtagc gcgggcttca gcctgcggag gttaatctgt gcaaggccaa aaccccacag
     5041 gctgaagcct atgccgcaaa gctacttcag attcccaaga ccttcgtaaa attgcgccac
     5101 agctttaatc ccgccgaaat aattttccag cgcaatgtgt tcgtcgggcg cgtgtgcatt
     5161 ttcatccggc aagccaaacc ccaacagaat gcatggcact ttaaatgtgt catgcatctg
     5221 cgtgacgaag ggaatggatc cgccttcgcg aataaaaacg gcgcgcttgc caaatccttt
     5281 ttccagcgca ttgtgcgcaa cctcaaaaaa tggatgtgtg taaggcgcgg cccagggatt
     5341 gccttggctg tgcacatcaa tcttaaattt cacggttttt ggcacgagct tcgcgatgtg
     5401 ccgtttcacg aggcgcgtga ttttatccgc atcctggttg ggcactagtc gcgtcgaaaa
     5461 tttcgcataa gccttcgatg ggattacggt ctttgcgcct tcaccggtgt agccgcccca
     5521 gatgccgttc aattccagcg tgggccgtgc accgagctgt tcgataatcg aaaaaccttt
     5581 ttcaccggtt agtccaggtg caccaacagt cttcttgaat tcactcgcct tccatgggag
     5641 cgattttaag cccttgcgtt cagcctttga caaattcgcg acgtcatcgt aaaagccggg
     5701 aatcgccacg cgggaattct tatcgtgcag cttggcgaac aattcacaca aaatggtgat
     5761 cgggttaggg accgcgccgc cgaaaatgcc tgagtgcagg tcctgcgccg ggcctgtcac
     5821 ttcaatctga taataattca gcccgcgcag agcataggtg atcgaaggaa ctccgggagc
     5881 gagcattccc gtatctgaaa ccacgagcgc atctgctttc aggcgcgcct ggttcgcgct
     5941 cacaaaatcc cacaggctca cgctgccaac ttcttcttct ccctcgatca tcactttcaa
     6001 attgatcggc agttttcccg cagttttcat aatggcgtcc agggcgcgca ggtgaatatg
     6061 aacctgcccc ttatcgtcag ccgagccgcg ggcgaataaa tttccgccgc gaatcgtcgg
     6121 ttcgaatgcc ggagatgtcc acaaatccag aggctcggcc ggctgcacat cgtaatgtcc
     6181 gtaaaataaa atggtcggtt tgccgggcgc gcgaagcgat tcgccgtaga cgagcggatg
     6241 cattttggtc ggcagaattt cgacgttgtc gaggccggcg gctcgcagtt tttcggccac
     6301 ccattttgcc gcgcgttcaa tgtccggttt atgttcggat tgtgcgctga cgctggggat
     6361 tcgcagaaat tcgtaaagtt cgttcaggtg ttcatcgtgc ttggaatcga tgtaatcggt
     6421 caggttcatg tgcagcctcc ggaaaattgc cggtcaggaa ttgtatcccg caaaatcata
     6481 gcgcgtaatc cattcacggg cactcgcaaa caagaggtat gggtgagatt ccgattcact
     6541 ttaggactat gttgcaaaaa gagaacacgt catcgacacg acgattgacg ccttggaagg
     6601 attgccgcag catggaatca ccatgcccga aaaagatttg gacaatgccc gccgcagcac
     6661 acgcgtcttc atgcgagttc gcgtcgttgt ggccggaaaa aattccgaca atcgccgctt
     6721 ccgtgaagcc tgcgagacca tcgtgatcaa cgctcatggc ggcctgcttt atctaaacca
     6781 gccgctcgat atggacgcca tgctggtcgt taccaatcct tttacgcagg aagagcagga
     6841 atcgcgcatc gtctttatcg gcgatgccac gaataaaggc cagcgagtcg gcatcgaatt
     6901 tcttacgccg gcgccgcatt tctggggcgt ggatttcgtg cccatggatt ggcccgcgcc
     6961 gcccgccacc gttcgctcgc gttaaatttg cgcttagcga ttccgtgata tcccaacttc
     7021 attttgaagg gagcttttca atttgcatct gcacttgcgt tctgaattcc aacggatgag
     7081 agaccaagtt gaaggtttcc ggtgtgccgc ccgttccgtg gattaccact gcgccatagc
     7141 cgagcactct ccccatcgca ctttcctgta cgcctatgct ttccactcgt gataaaagca
     7201 gctcgatggt tctgcgcgcc gccagtccca ctttcacaaa aacgcgttta ttggttacgg
     7261 tcatttctgt tgaatttctc atcacgatgc ctcgaacgac gaaaaacagc gccaatgcaa
     7321 aaaaaactcc tcccaggacc attgtcggtt catgagcgga actgcgatca ccggtagcac
     7381 gactcgaaag aataaacatt cccactcccg tcaacgcgaa aagaattccg agcacaacgg
     7441 gcgcgacaag gacgatccag tgcaggcggg tttcgtagac aatcgattct ccatcgatga
     7501 ggtgcttttc aacgtagctc atggcgctcc ttggcgattt gcggcctgcc cattaccagc
     7561 ccacgaaatg gataaccaac aaattagcaa tataaagcaa agcacgcagc ggagttgtga
     7621 tttttgtggc gccacgccgg cgccacaaaa cgcgttggac tggcacttta agagaacagg
     7681 attgcaacgg atggaattca aaaccgctca tcaagagtcg gtagctaaaa ttcggttaca
     7741 atgctcatac gatagacttg gcgcaatgga ttttctcaat caataaaccg gcaagaggta
     7801 ctgattacct atgaattcca aacaatccgc gctatcgcca aaaacaaaaa ccggagcgaa
     7861 cttaacccgg cgcggcattt tgcggggtgc ggccggcgcg gttgccgcgg cgagcctttc
     7921 gcgtatctcc ctattcgctg aagattccgt cagcccgccg atggcgcggc tcagttccta
     7981 catgagcgaa gcgcgtaacc acgccctgcc ggatgacgta acggagaaag ccaagcatca
     8041 tattctcgat acatttgccg cgatggtttc cggatcggaa ctggtccccg ggcaagcggc
     8101 gcttaaattt gccagaaatt atgggggtga aaaaattgcc acggtgattt gctcgaatat
     8161 tttgtgcggg cccatggagg cggcgctcgt caacggagtg cttggccatt cggatgagac
     8221 cgatgacgct cacgctcctt ccggctcgca tcccggcgtg gcgatcgtcc ccgcggcgct
     8281 tgcggtcggc gaaaagttcg gtatcagcgg cgctgagttt ttgcgcgcca tcaccctcgg
     8341 ctacgatgtt ggcacgcgct tcgtgatgac catgggcgga gccgctattc aaagcgagca
     8401 gcaccgcagc acgcattcca tcgccggaat ttttggcgcc gcggcagccg cggcaagcgc
     8461 agccagcttg aacgagcagc agatgcgctg ggtgctcgat tacacttcgc agcaatcctc
     8521 tggcacagct gcatggcaac gtgacacgca gcatatcgag aagggtttcg tttacgcagg
     8581 catgccggcg cgcggcggcg tcacgtccgc gctactcgtt caatcgggct ggaccggcgt
     8641 cgacgatgtc ctctccggcg ctgacaattt tttcatggct tatgcgccgc aatcgaatcc
     8701 cgcatcacta gtggacaagc ttggcgaacg ttttgcggta acggaaacgg atatcaagaa
     8761 atggacggtc ggagctccga ttcaggcggt gctcgatgca gtggatattc tgatcaagcg
     8821 acgttccttc tccgcggatg atgtgaaaga ggtcgttgtc cggcttgccc gcacgcaagg
     8881 aactgtggtg aacaaccgcg agatgccgga catctgcgtg cagcacatgg tagccgtcat
     8941 gctgatcgat aaaagcgcgt cgtttaaatc agcgcatgat aaagccagaa tgcaggatcc
     9001 cgccgttctt cgcgtgcgcg ccaaagtaaa tctggtgctg gatgatggtg aactacagcg
     9061 cgccttgccg cgtcgcgaag ctatcgtgga gattactttg aatgacggaa cgaggttaaa
     9121 cgaacacgta aaaacggttc gcggcggcgc tgcaaatgca atgacgcgcg aagaagttgt
     9181 cgcaaaagcg cgcgagctga tggttccagt tctgggcgct gcaaacggtt ccgccctcat
     9241 agaaaaaatg ttgaaccttg aaaatgtgaa gagcgttcaa gaattgcggc cgttgctgca
     9301 actcgtctga tgaagctgca agatggtttg gtcttatgtg gcataggctt cagcctacgg
     9361 aatttagcct ttggaaggac taaaccccac aggctgaagc ctatgccacg taagagcaac
     9421 tgttcgattc agtgatctgg cggttgatta tttttttggc gtaatcggca gtagaagata
     9481 ggggcgctgc tctttgctcg tgtgaagcgt ggtcttcccc gaaaaaatat ttgccgggcg
     9541 atccgtcgga tcatcatgca ggaacggccc gcatccgcgc agctcgttct tgaagttgga
     9601 aagctttccc cctctgcatg cgtgctcgta atcttttccg cgaatggtca gcgcaattcg
     9661 gtaacccacc ggtacgacca tgctggttgg ccatatctcc acatccatct ctacagcaat
     9721 gccaggtttc agtggctgac gctcgtcatg cgtatgatac ggacgccacg gtttcgaaat
     9781 tgctgcatcc agtttccggt tcgaagcgcg cagccaacct tgcccaatcg gtgtgtgcgg
     9841 atcgattgct ccctgaaaca caacttcctt catatccgga gaaaacacac gcagcaccgc
     9901 aaaaatatcg gcgtcctcgg tagtcgaaga aagaaataat ttcagcgctg agggccccgt
     9961 aatttcggtt tcagccgtga ggggcggcgt catcaaagtg acgccatcgc ccatcgcgtc
    10021 aaacgaaacg cttgcaggat ccgccgtcag cggtacagcc gcgagtccgc cgttcgcgaa
    10081 atcgaaataa aacttcgtcc atttcgtgcg ggccaggggc cattcgtttt ccacgcgctc
    10141 ttcgaaacga tcgaggtggc gcacttgcag caagacttgc ggctgctgat cccacccgtt
    10201 cttttcacct tttaaaaaat aatcgaagaa acgtttctgt agatcgcgcc cgtaatcagt
    10261 ataaaagtgg gtccagtgct cgatgccatg cgactccagc catttgtgtg tggatgccgc
    10321 gcgcatgaaa ccttcgaaat ttccacgcgg atgcaatcct tgtccgcccc aattcgcagc
    10381 agaaagaaaa ggcacggtca ccttagacca gtcgggcgag cggtctttgc tgtactggtc
    10441 atcgagcgga tgcgcaaaaa ggtcagcacc gaaattacag cgattggcgg cgagttccgc
    10501 ttcggtcagc gtttctgggc cggaaacaag atcgcctgta gcgcggctgc acagcccgcg
    10561 cgtaccgagt ccgtgctgca cggttttcac ctgcatgtcg taccagttcg cccagaatgt
    10621 gcagtagatc ccgccgtgat gcgaagtatc gcggtaccat tccgccgctc cctcccacac
    10681 acacatggcc gccaaatgcg gcggctgctg cgacgccacc tgccactgat tgatcgcgta
    10741 atacgaaatg ccgttgaggc cgacttttcc ggtgctccat ggctgttcgc ctgcccatgc
    10801 aatgcaggca acaaaatcct tcgtttcgcg tggcgaaaat ggatcgacgt agcctggcga
    10861 gcgtccgcag ccgcggctat ccacgcgcac gcaaacgtag ccatgcggca cccacttttc
    10921 cgggtcaacc acttcccagt tttgatacaa gttggttgag cccgcgggca cgtcgggatg
    10981 ttcgcgagcc atcgtttccc aggcgtcggg atatccgtct tgaaaagaga gacccttcgc
    11041 gtaaggtccg tacgtgagca ggacggggaa acggccttcg ttcactggac ggaaaacgtc
    11101 ggcgcgcagc acgatgccgt cgtccatcga gatcggcgcg tcccagtcga ttttcattcc
    11161 atcgcggatt tcagtttttg attgtgcgtc aggtcgtgaa ctcatcttca tttcctcatg
    11221 aaacaaatgg gaatcaggag ggcaaaggtt ttcctttggt ctccatgcca aacggcagca
    11281 gcaacagtcc tacgaggaat gcggcggccg tcatggccac gggaattccc aacgtttgaa
    11341 aatgccgcac tcccacgccg accagaaatg tagcgcccgc tcctgcaaag cgtcccacat
    11401 tttgcgcgaa agccagcgcg ctggcccggc attccgtttc atactgctca ggcaaccaaa
    11461 aagaatagac ggtgtaattt gcgccgccaa tcccgagaaa gaacaggcac acaaaaaacc
    11521 atttgagcgt gtctgtctgc agatagaaaa catatccgaa ggtaatcacg atcaccacaa
    11581 acatcagcga ataaaaaatt gcctgattta ttttgcgtcc aaagcgatca gcgattgcgg
    11641 gcacgctcag cgcgccgaga atcgttccaa tggaaagaat tcctgttccg taagatgcga
    11701 gccgcgccga ttgaaatggc gaatggcccg acatcgcggc gagataagta atcgcagcag
    11761 gcacgtacac ggagccggcc cacagcccga tcgtggaaac gagttggtac atgaggttca
    11821 tcactgtgcg caggcgatat cgcacggaaa agatttccat gaaggagcgc tgcaagctcc
    11881 acctttcgcc cagctttcca cgtttgcttt cccaccgcgc cggttctttc attcccctgc
    11941 gaatcagaat cacgagcagc gccggaacgc cgccgaccac gaacatccat ctccacccgt
    12001 aacggctgcc gacaaaataa ttagccagcg ccgccagaaa aaatccaaag taatagcccg
    12061 tatgcatcat ccccgcgccc atcgtgcggc ggtcttccgg ccactcttcc gagatcaacg
    12121 ttgcgccgat cccccattct ccgccgatgc cgattcccgc gagaagccgt agcgttgcaa
    12181 gcatccagat gttggtggca aacgcgctca gcaaagtaaa aagtgaaaaa caaataatgg
    12241 tgagcattaa agcgcgcacg cggccaaaac ggtcagccac cggcccccac agcagcgcta
    12301 atccccagcc aaccagaaaa agtgcgaaga gaattccgcc ataataccca acattcgctg
    12361 tagtggcagg aattcccgat cgaggcagta gatcgcgtaa cgcaggaacc agaacgagcg
    12421 cgtagatgaa cgagtccatc ccatccagaa cccacccacc ccacgccgcc cagaatccgc
    12481 ggatttgatt tgacgtgagt ggagtccgag tcgaagtcat tgtgcggtga aattagtgta
    12541 agtaaataag ccgcagtgct gaatcgccga cgcattgcca atttgagtcg tgcataagat
    12601 tggccaatgc cgcgtttagt tccatttgcg catgggatga taccaccgca aacagtagat
    12661 tctccgtgtc tccctgcatc cgcattacag gaaggttggt tttcgtagcg ccggcgccac
    12721 gaaaaccgtc acgtcgatta ctatctctgc cttcccggat cgtgccagcg tcctcgaaac
    12781 agttcctgat cgtccttgcg ggaaactcag ttacaatact tttatctgca aaatgataat
    12841 tgcgcggcgc aaacgctaaa ttattccagg attatggggg cacacgaatg aaacgcagtc
    12901 aaaaagcgaa tttgccaatc ggaaaatctg aaaaaaatct cacccggcgc gatctggtaa
    12961 agcacggtat atgggttctt gcggcgactg ccattccagg cgcggtgaaa ttagccgccg
    13021 aagacattag ccccatgatg ctgaagctca gcacttacat gagcgaagcg cgcaataacg
    13081 cgctccccga taaagtcctg caggagacca agcaccatat cctcgatacg tttgccgcga
    13141 tgatttccgg ttctgaactt ccgcccgggc gcatggccct gaaatttgcg cacgcctacg
    13201 gaggtccaag catttccacc gtggtggcgt cggacattct tatggggccg atcgaggcgg
    13261 ccatggtcaa cggggagctg gcgcattccg atgaaacgga cgatgactac acgggtggcg
    13321 gagcgcatcc aggaagcgcc gtgattcccg ccactctcgc ggttggcgaa aaaatcggca
    13381 ttactgggca gcatttcacg cgggccgtcg cgcttggata tgacattgga atgcgcgcat
    13441 tcaaaacagt tggcgcgggc attctgaaag atacgcacgt cctggtgggc acgtttggcg
    13501 cgtcggccgc ggcaggttgc gtgctaagtc tggacgcgca gcagatgcgc tggctgctcg
    13561 attacgctgc gcagcaggcg ggtgcaggat tcgcaacctg gcggcgtgac accgatcaca
    13621 tcgaaaaagg ttttgtgttc ggtggcagca cggcgcgcaa tggagtcacg ggcgcgctcg
    13681 tggttcagct tggctggacg gccgtcaacg atgtgatgtc tggcccggac aatttcgtca
    13741 tgtcctacga tcccaaagcc gatcccgcaa aatttgtgga aggacttggc gagcgttacg
    13801 aaataacgct tacaactctg aaacgatgga cgacaggcgg ccccattcag tcgccgctgg
    13861 acgcgctaca gctcatcatg aaaaaacatc ccttcgagcc ggatcaagtg aagcaagtag
    13921 tggtgcgaac cgcgaccagc gccgcgtata cggtgaacaa ccgcgagatg cctgacattt
    13981 gcctgcaaca catgatcgcg gtgatgatca tcgacaaaac cgcttccttc aaagcggcgc
    14041 atgacaaagc tcgcatgcag gacccggcca tccttcgcgt acgcgcaaag gtgcagttga
    14101 tcggcgatga agaatttgag cgcctgatcc cgaagcgagt tgccgttgtg gaagttacac
    14161 tgaatgacgg aaccaagttg agcgagcgcg tcgaatccgt gcgcggcact ccggaaaatc
    14221 cgatggaccg cgaagaagtt gtgtccaaag cgcgcgatct gatcattccc atcctcggct
    14281 taaaaaagtg cgacaccctc gttgataaat tgctgaacct cgaaaatgtg aaagacatcc
    14341 gggaacttcg acccttgttg caacgcactt aaaaactgcg aagttatgta gcgctcgcct
    14401 tcaggtgagc accttggatg gagcaacaat gcctgactga aggcaggcgc tgcaaaggca
    14461 tgggtaattg ttgtaactaa cggagtgctt ggcggaggga acatgaaaat tatcgtaaga
    14521 attttgagcg caatttttgt gctcatgggc accatctggt ttctacaggg aataaatgta
    14581 ttgccgggca gttttatgac cgggcaatta cgctgggccg tgcgaggcgg aattttattt
    14641 gtggtgggtg ttgcgggcct gctatgggcg aaacgggacc ccaagcatgt ctgatgaaat
    14701 gcgccgcatt cttccagtag cgctcgcctt caggcgagca tctgcacgaa tcagcagtgc
    14761 ccgcctgaag accctacaca aaagaaacgg aacgcgtttt accgtctgtc gtggtctccg
    14821 ccgtgatccc caccatgatc gtgcccgtga tccccgccgt gatcatttcc gtggccgcga
    14881 tgttcttcag gtgcacggcg tccttctcgc tctgcgccgc gttctcgctc tgagcgctgt
    14941 tccagttcat ggtgctcagg cgcgcgctcc cctggtccgt atcggtgtac ccctgcatac
    15001 ggatgagcgt attcaccgcg gttgtaccag gtccggcccc agcgcgcgtt attaataaac
    15061 actccgtggc cgccccaatc gaagcggtta taaccccagc cccatggccg aaaggaaagt
    15121 ccgagcgaga cgccaaagcc gaaacgaatc gcgccaccca caaaaaagcc gggacgcggc
    15181 gcatagaaga caacacgcgg atcataggag ggaactacga caaatccggg attgacgggc
    15241 atgatcgtaa tgttcggccc gccgccaacc accacctgcc cgttgcttcg cagatatccg
    15301 tagtcacgag ccttgcgacg gtcgcgctgg actgcatcca ttacatcgcc ttgctgcgcc
    15361 aaaaaggcat ttccgaggtc ggtcgtccaa ttcatatccg aagccatcat ttcgagcacg
    15421 gatggaaagg gcagcaatgc ttggacgctg gggtcccatg gaagctggtc gccttggatc
    15481 gcgtcggcca gcgcttgtcc cgtgaggtaa tgatgctgat cggcccagcg tgcggcatcg
    15541 ggaatctgat cagggtatgt ggccccggcc aaaatctggg ctaccagcgg atcagggtaa
    15601 agcgcgatac gtgagaccag ttgttccagc tgaggtggcg caaaggacgg cggcccttgg
    15661 gcgaaggcag gtaccgcggc caataccagt aacatcatta ttagtgcgaa ttttatgatg
    15721 cgcatcttct tctcctactt tcgatttata cggctcattc gttccattcc gcaatacgca
    15781 atggtcctga tttctgtaat tgaaaatggt agcgcacctg tttccgggtt gtccgcaaag
    15841 acttgagacg ggttgctccc tcgacatcag gagcaggaga acacgcgctt gactctcact
    15901 ttccactagc agaaactcag ctgagttcat tcacagtgaa atttccgcgt gcggtgataa
    15961 gtgatggcgc tcccagtcaa aaatgaagga ttcaatacgc cctatgaagt cgacccggcg
    16021 gtgcgcatcg ggcacgttca tttgaaggtg gccgatctca aacgagcggt cgcattttat
    16081 agcggcgttc ttgggcttga agtcatgcaa aagctcggcg aggaagccgt atttctttcc
    16141 gcgggtggct accatcacca cattgcgttg aatacatggg aaagtctcgg cggttcgccg
    16201 ccgcccgtcg gtgctaccgg gctttatcac acggcaattg tttatccgac tcgcgctgct
    16261 ttggctaccg cattgcgtcg tgtgctggcc gcgcgaattt ccctcgaagg tgcggcggat
    16321 cacggggtaa gcgagtccat ctatttgcgc gacccggatg acaatggcgt ggaactgtat
    16381 tgcgataagc caaaagaact ctggccgcgc gatgcgaaag gtgatttggc tatgtacaca
    16441 cgcatgctcg atttaaataa attgctcagt gaagctccat cgggatcggc cgtgtctacc
    16501 ggccccgaag atctgtaatt tgtagcgccc gccttcaggc gggtaccgtc gattcactta
    16561 gatgaccgcc tgaaggcggg cgctacatgg accaaataca atttttcaca cagactcttc
    16621 ggcctgttgc acttgtggca cgctaaaaaa ttaaaatcca acaggcgcaa gcctgttctg
    16681 gttattttcg acaggggaac ttcgatgatt cgacgggatt tttgcaaaac atcgctcgcg
    16741 ctcgctggat tggcgctagg caaagggccg gccgaggctc tcgccagctc tgccgtggca
    16801 agtcccgcgg aagaattggc caaagcgccg ggcctgacga aatacgtttc cgaatttatc
    16861 gtgaatacga agtacgaaga cattccggaa aatgtcatca ccatcggcaa gaagaccatg
    16921 ttggatggtt tcggattggc gctcgccgga tcagcttcca ctgccggtcc gcgcatccgc
    16981 caatatattg aatcgctcgg tccgtgtgga ggcagcgcaa gcattatcgg cacaaaaatg
    17041 aaagtgcatc cgcgctttgc cgcacttgcc aatggcatct ccattcatgc cgacgattat
    17101 gacgataccg gttccgcatt ccacgttgcc tcgcccgtgc ttcctccgtc tttcgcgctg
    17161 tgtgaggtgg gcaagcgaag cggccaggat ttaatgctgg catttcatgt gggcgtcgaa
    17221 gtcggaaata aaattggaga cgcgatgtct ccccgccacc agcaagatgg ctatcacacg
    17281 accggcacga tcggaaccat gggcagcgcc gcatcttgcg caaaactgcg cggcctggac
    17341 acggcaaaaa ccgccattgc tctggccatt gccggatcgg aagccagcgg acttcgcgac
    17401 aattttggct ccatgacgaa gccctttcac gctgggcatg ccggcgaaaa cggaaccgta
    17461 gctgtcgatc ttgcggcgat cg
//
LOCUS       DASZMB010000047         3792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4180884, whole genome shotgun sequence.
ACCESSION   DASZMB010000047 DASZMB010000000
VERSION     DASZMB010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4180884"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="VG272_02055"
     CDS             complement(<1..614)
                     /locus_tag="VG272_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12491.1"
                     /translation="MPDYMFQLESRLSPEQRAAMVRIQELAVQFETNLYLVGGAVRDM
                     ISGMSIRDLDFTIEGNPARILRELEKGGAKIISEDDSLRTAELVIAGDVDASISAARE
                     EVYARPGTKPEIRFSTIMEDLRRRDFSINAIAISLNANSRGLLLDPTNGLADLERREI
                     RALTIHSFTNQPARLLRALRYEARMGFKLEQRTSDWFKLAIERE"
     gene            complement(718..1617)
                     /gene="xerD"
                     /locus_tag="VG272_02060"
     CDS             complement(718..1617)
                     /gene="xerD"
                     /locus_tag="VG272_02060"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HWF12492.1"
                     /translation="MESTIHVFLSYLRVERALAQNSILAYGRDLKRFSEFLRKRQKNK
                     VEEVTREDIVDFLSNLYKEKMDSRSVARYLVSLRGLYKFAMMEGLVNTDPTENFESPK
                     TRHSLPTYLRVEEINKLLEAPNLSTPLGLRDRAMLEILYSTGLRVSELLNLRISDIDM
                     RVGCVRCIGKGDKERLVPIGRKAIEAIEQYLAHGRPKFARPSSPPPHSHVLLLTSVGR
                     RLSRVGIWKIMHNYGVRLGLRGRLTPHKLRHSFATHLLERGADLRSVQLMLGHADIST
                     TQIYTHVVEERLKEIYKAHHPRA"
     gene            complement(1624..3264)
                     /gene="murJ"
                     /locus_tag="VG272_02065"
     CDS             complement(1624..3264)
                     /gene="murJ"
                     /locus_tag="VG272_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HWF12493.1"
                     /translation="MTGGLVLLTETVTHKRQILKSASIISIITVVSRILGYVRDQRLT
                     LLLGTTGLADSFVLAYRIPNVLRRLVGEGSMTAAFIPVFTDYMRNRSRGETWEFANRL
                     FWTFCVILSAFTVLGVIFSPLFVRFFSMFGKNQVQFEAIYLNRLMFPYILFIGMAAMA
                     MAILNCFHIFGPPAATPILLNISFIVFSIAAVWKHFSSPAAALAVGVLVGGIFQFFWQ
                     VPQLVRRGMDFKFTVSFMDPGVRRVARLMIPGFLGMGIAQINLLVDTIFANAKIMPEG
                     SLVSLYVADRVTELVLGGYAIAVATAILPMMSHQAAAGDHEGMKKTFLFALRIVSFIT
                     IPAAVGLVILREPIVQVLFQHGRFVAESTRLTARALLYYSLGLPAFAAVKLIVPAFYS
                     TRDTRTPVRVAVLAMLANVLLNVIFLFYFFAKLKNGGPALASALAGYFNVFALFVVFR
                     LRFGRLGTRDVAASLGKIVACASLMGVFCWGALKYSQFDSIEHFLPRLLVFVGLIGSA
                     TLTYLGLAWAFRCNEISEVYGIAFHSAGAETGKQGIVG"
     gene            3370..>3792
                     /locus_tag="VG272_02070"
     CDS             3370..>3792
                     /locus_tag="VG272_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12494.1"
                     /translation="MKVRTPSSTHKKVVVRFFDRNDLRGYLNPSRLGETDIIELLTPD
                     GEQRNFELKDVRSIYFVRDFGENFEPERKAFLSRPKLDGLWVRLKFRDEDTIEGVVPN
                     DLLSLLDRGVQITPPDLHGITLRIFIPRTAMRELKVLGV"
BASE COUNT          964 a         1063 c          975 g          790 t
ORIGIN      
        1 aattcacgtt cgatggccag cttgaaccaa tccgacgtgc gctgctcgag cttaaagccc
       61 atgcgcgctt catagcgaag cgcgcgcaag agccgcgcag gttggtttgt gaaactgtga
      121 atcgtgagcg cacgaatctc gcggcgttcg aggtctgcca aaccgttcgt tggatcgagt
      181 agcaaaccgc gcgaattcgc gttcagcgag atggcgatgg cgttaatcga gaagtcgcgc
      241 cgacgcaagt cttccatgat ggtggagaaa cgaatttcag gtttcgtgcc gggccgcgcg
      301 taaacttctt cccgcgcagc agaaatgctc gcgtcaacgt ctcccgcgat gaccagctcc
      361 gcagtgcgca gcgaatcgtc ttcgctgata atttttgccc cgcccttttc cagctcgcga
      421 agaatgcgtg cgggatttcc ttcgatcgta aaatccagat cgcggatgga cattccggag
      481 atcatgtccc gaaccgctcc ccccaccaaa taaagattgg tctcgaactg aactgcgagt
      541 tcctgaatgc ggaccatcgc ggcgcgctgt tcaggggaaa ggcggctttc gagctggaac
      601 atataatcag gcatgcattt accgtcctcg gccggatacg ccacttactt ctctgcgtct
      661 ccgtacccga tcaattttta tttccgcggg cataatcccg cgaactgtat ttagccttca
      721 cgcacgcggg tgatgcgctt tataaatttc tttcaagcgc tcctcgacca cgtgcgtgta
      781 aatctgtgtc gtggaaatat ccgcatggcc cagcatgagc tggaccgagc gaaggtccgc
      841 accccgttct agaaggtgcg tggcaaaact gtgacgcagt ttgtgcggtg taagccgccc
      901 gcgcaaaccc aagcgaactc cgtaattgtg catgatcttc caaatcccca cgcggctcag
      961 tcttcggcca acactcgtca aaagcagcac gtggctgtgc ggcggaggac ttgagggccg
     1021 ggcaaacttg ggacgcccgt gcgccagata ttgctcgatt gcctcaatgg ccttgcgccc
     1081 aatgggtacc aggcgctcct tgtctccctt acctatacac cggacgcagc caacccgcat
     1141 atcgatgtcc gaaattcgca ggttcagcaa ctcggatacg cgcaatccgg tggaatacag
     1201 aatttcaagc atcgcccgat ccctcaatcc aaggggcgtg gagaggttcg gagcttccag
     1261 cagcttatta atttcttcca ctcgcaaata agtgggcaag ctatgtcgag ttttgggaga
     1321 ctcgaaattt tcggtcgggt cggtgttcac caggccttcc atcatcgcga acttgtacaa
     1381 cccgcgcagc gagaccagat atctggccac ggagcggctg tccatctttt ccttataaag
     1441 gttactgaga aaatcgacaa tatcttcgcg cgtaacctct tccactttat ttttctgacg
     1501 tttgcgcagg aattcagaga accttttcag gtcacgacca taggcaagga tcgaattctg
     1561 cgcgagagca cgttcgacgc gcaaatagct cagaaaaaca tgaatcgtcg attccaattc
     1621 cgtttacccc acaattcctt gcttgccggt ttccgcgccg gccgagtgaa acgcaatgcc
     1681 gtacacttcg ctgatttcat tgcagcgaaa cgcccacgca agcccgagat acgtcagagt
     1741 cgccgagccg atgagtccga cgaacaccag caatcgtggg aggaagtgtt caatactatc
     1801 aaactgcgaa tattttagag ctccccagca gaagacgccc atcaagctgg cgcacgccac
     1861 aatttttccg agcgatgccg ccacatcccg cgtccccagc cttccaaatc gcaaccggaa
     1921 aacaacaaat aaagcaaaga cattgaaata tccggcgaga gcgctggcga gagctggtcc
     1981 gccgttcttc agtttcgcaa aaaaataaaa taggaatatg acattcagca acacgtttgc
     2041 aagcatggca agaacagcca cgcgcaccgg cgttcgtgta tcccgagtcg aatagaaagc
     2101 cggtacgatc aatttaaccg ccgcgaatgc cggaagcccc agcgaatagt acagcagcgc
     2161 gcgcgccgtc aagcgcgtgg actccgccac aaagcgccca tgctggaaca gcacctgcac
     2221 gatgggctcg cgaaggatca ccaggccaac ggccgcaggt attgttataa acgaaacgat
     2281 ccgcagcgca aaaagaaacg tcttcttcat cccctcatgg tcgccggcag ctgcctggtg
     2341 cgacatcatt ggcagaatgg ccgtcgccac agcgatcgcg tagccgccca gaactagttc
     2401 tgtgacgcgg tcggccacat atagagaaac caggctgcct tccggcatga tcttggcatt
     2461 cgcaaatatt gtatccacca gcaggttgat ctgtgcgatg cccatgccga ggaacccggg
     2521 gatcatcagg cgcgccacgc gccgcacgcc aggatccatg aacgacacag taaatttgaa
     2581 gtccatgccg cgcctgacga gctgcggaac ctgccagaag aattgaaaaa ttcccccgac
     2641 cagcaccccc acggcgagag ccgcagccgg actcgaaaaa tgcttccaaa ccgccgctat
     2701 cgaaaacaca ataaatgaga tattgagcaa gatgggagtg gcggcaggag gtccaaagat
     2761 gtgaaagcag ttcagaatgg ccatggccat agccgccatc ccgatgaaca ggatgtaggg
     2821 gaacatcaag cgattcagat agatagcttc gaattgcacc tgattctttc cgaacatcga
     2881 aaaaaaccgc acaaacagag gtgaaaaaat cactccgagg accgtgaaag ccgataatat
     2941 cacgcaaaat gtccaaaaca aacggtttgc gaactcccaa gtctctcccc ggctgcgatt
     3001 cctcatgtag tcggtaaaga ccgggataaa agcagctgtc atggatccct cgccaaccag
     3061 ccgacgaaga acattgggga ttcggtatgc cagcacgaag gaatcggcca agcccgtggt
     3121 ccctaaaagg agggttaagc gttgatcacg gacatagcca agaatccggc tgacaaccgt
     3181 tataatgctg atgatggaag cagatttaag gatctgccgc ttatgagtga cagtttcggt
     3241 caaaagaacg aggcccccag tcacagcctc tgtctaaacc tttgataaac atacacttga
     3301 cagcaaaagc gcgcttaaca taacatactt gcgctcgact cgcaaggccc gcccttcgag
     3361 tctgctcctg tgaaagtacg aactccatcg tctacgcata aaaaagtggt cgtgcgattt
     3421 ttcgatcgca atgacttgcg gggatacctg aatccaagta ggttgggcga aaccgatatc
     3481 atcgaactgc tcactccgga tggagagcaa cgcaattttg agctgaagga tgtgcgcagc
     3541 atttatttcg tgcgggattt tggggagaat tttgaacctg agcgcaaagc gtttctttcc
     3601 cggcccaagc tggacggatt atgggttcgc ctgaaatttc gcgatgagga caccatcgaa
     3661 ggtgtggtcc ctaacgattt gctttccctg ctcgaccgcg gagtgcagat tactcccccg
     3721 gacctgcatg ggatcacctt gcgaatattc attccgcgga cggcgatgag ggagttgaaa
     3781 gtcctcggcg tt
//
LOCUS       DASZMB010000048         2688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2524571, whole genome shotgun sequence.
ACCESSION   DASZMB010000048 DASZMB010000000
VERSION     DASZMB010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2524571"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VG272_02075"
     CDS             <1..193
                     /locus_tag="VG272_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12495.1"
                     /translation="EFPLPGRGINLRHISLDERDGVTRIVVGYTRNSKIARMQIRSKE
                     ELQALKSQAQNLSAQGATH"
     gene            354..1139
                     /locus_tag="VG272_02080"
     CDS             354..1139
                     /locus_tag="VG272_02080"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWF12496.1"
                     /translation="MGRMETFLLGISAAILAMFLAGPIVTMISSPLPASPAQESAVTT
                     ETVHFDSGGFNIDAFVAKPSGAEKHPAVLVIHDNLGLNDSMREIAKRFAAAGFVALAP
                     DFTSRRGGTKTPDQMAQAVAQLSPNLTVQDARSALDYLQKDSAVDADRISTVGFGWGG
                     WRSFMLAASTPELYRAVIYSGTTPSQSFDTVHATILAHYAQYDFRTTGNVLVTEKAMT
                     EAGKHFSYFVYPQAYRAFYVPGTQYNAEVAKVAWARTLDFLRK"
     gene            complement(1163..1447)
                     /locus_tag="VG272_02085"
     CDS             complement(1163..1447)
                     /locus_tag="VG272_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12497.1"
                     /translation="MLVVRNCFTAKPGQATKLAAQIKEAAATGKFPRYRVLTDLTGEF
                     NRVVLEFEVESIAEFEKQLKDYGTNEAFRAKMKGYTDLWITGSREIMQII"
     gene            1715..>2688
                     /locus_tag="VG272_02090"
     CDS             1715..>2688
                     /locus_tag="VG272_02090"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF12498.1"
                     /translation="MNKRQDSMNENAAVRPSLRYESHSESVADASRRDVLRWMAIAGA
                     GACLPAGKLLAGAMSPASSSGRIDVHTHTVPPFYTKIMEKEIMATGHPLPAWSPAAAI
                     EMMDKTGVAASMLSPMTRVVQDSFSDKSERARKLARQNNDYTQQLVRDYPGRFGNFAA
                     IPLPDTDGSLKEIEYALDTLKADGIGLWTSYLDKWPGDPMFAPVYEELNRRKAIIFLH
                     SAPPSCCRTLQPGVADSVVEYDFDIARAVVSYLKSDSFRRYSNIRFIFPHSGGTLPVL
                     ANRVSETLPEKRSEKDSAEIMDELKGVYFDVAHAAYPAPLAAITKVIP"
BASE COUNT          611 a          741 c          722 g          614 t
ORIGIN      
        1 cgaatttcct ttgccgggac gcggaattaa tttgcgccat atctccctcg atgaacgaga
       61 cggagtgacg cgcatcgttg tgggctacac gcgaaacagt aagatcgcgc gcatgcagat
      121 tcgctcgaaa gaagagctgc aagcgttgaa gtcgcaggcg caaaatttat cggcgcaggg
      181 agccacgcac taattgaaac gccgccttgg agcggcttca gccggttttt gtggcgcagg
      241 cgtcccgccg gcggcttttg aattgcgtga cgctctaaac ccgccggcaa gatgccggca
      301 ccacaaggcc gcatccgcag tcgcggcata ttcattcagg gagagtgaaa tacatgggtc
      361 gcatggagac ttttctgctg ggcatttctg ctgcgattct cgcaatgttt cttgctggcc
      421 caattgtcac catgatttcc tcacctctcc ctgcctcacc cgcgcaagaa tctgccgtca
      481 caacggagac agtgcacttt gattctggcg gattcaatat cgatgcgttt gtagcgaagc
      541 cttcgggtgc ggagaaacat cccgcggtcc tcgtcattca cgacaatctt ggactcaacg
      601 attcgatgcg cgaaatcgcc aagcgatttg cggcagcggg atttgttgca ctcgcgccgg
      661 atttcacttc gcgtcgcggc ggcacaaaaa cgcccgatca aatggcacag gctgtagcac
      721 agttgtctcc caacctcacc gtgcaggatg cacgttcggc gctggactat ttgcaaaaag
      781 attcggccgt ggatgcggac aggatttcaa ctgtgggatt cggctggggc ggctggcgaa
      841 gcttcatgct ggcggcttcg acgcccgaat tgtatcgcgc cgtaatctat agcggaacga
      901 cgccgtcgca aagtttcgac actgttcacg ccacgatcct tgcgcattat gcccagtacg
      961 attttcgcac taccggaaat gtgctggtca ccgaaaaagc aatgaccgaa gccggaaagc
     1021 atttcagcta tttcgtctac ccgcaggctt accgcgcgtt ttatgtcccc ggcactcaat
     1081 acaacgcgga agtggccaag gtcgcgtggg ccaggaccct ggatttccta cgcaaataag
     1141 aatgagcaaa gatcgcgccc ggttaaatga tctgcataat ttcgcggctg ccggtaatcc
     1201 acaaatccgt gtagcccttc attttggcgc gaaaggcttc gttcgtgccg taatctttca
     1261 actgtttttc aaattccgcg atgctctcta cctcaaattc cagaacgacg cggttaaatt
     1321 cgcccgtcaa atcagtgaga acgcggtacc ggggaaattt tccggtggca gcggcttctt
     1381 taatttgcgc ggcgagcttg gtcgcttgac ctggtttggc agtgaaacaa tttctcacga
     1441 cgagcatgaa ctttcctcct tgaatatttt tcctgcaaag agctgcttca tctttctgta
     1501 agcccgactc aaattgcagg aattaattgg gtgcagagtg acatccgcat ataaatattg
     1561 tcaagacgta tcgactgcga cgtatcgact gcgatctcgc attgggaatt cgaccgcgca
     1621 aaatcacttt cgcattgttt gcggatgcca cgaggtgtag attgccgttc aatacaaatc
     1681 aaacattcag gttggcaagc gaatggagga cgccatgaat aagcgtcagg attcaatgaa
     1741 cgaaaatgcc gcggtgaggc caagccttcg ttacgaatcg cattccgaat ccgtcgctga
     1801 tgcttcgcgc cgcgatgtcc tgcgatggat ggcaattgcg ggagctggag cgtgcctgcc
     1861 tgctggtaaa ttgctcgcgg gtgcgatgtc ccctgcttct tcctccggtc gaatcgacgt
     1921 gcacacacac accgtgccgc cgttctatac aaaaattatg gaaaaggaga ttatggccac
     1981 cggtcatccg ctgcccgctt ggtcgccggc cgccgcaatt gaaatgatgg acaaaaccgg
     2041 cgtagcggct tccatgcttt cgccgatgac acgcgtggtc caggattcct tttccgacaa
     2101 atcagagcgc gcgcgcaagc tggctcggca aaataacgat tacacccaac aactggtacg
     2161 cgattatccg ggacgattcg gaaattttgc cgccatcccg ctgccggaca cggatggaag
     2221 cttgaaagaa attgaatacg cgctcgacac gctcaaagcg gatggcatcg gcctgtggac
     2281 cagctatctc gacaagtggc ccggcgatcc tatgttcgcg cccgtgtacg aggaactgaa
     2341 ccggcgcaag gccatcattt ttctgcactc cgcgccgcct agttgttgcc gcacgttgca
     2401 gccgggagtg gccgacagcg tcgtggaata cgatttcgat atcgccagag cggttgtgag
     2461 ctatctgaag agtgattcgt tccgccgcta ctctaatatc cggttcatat tcccgcactc
     2521 tggggggacg ctgcccgtgt tggccaatcg cgtgagcgaa actctgccag aaaaacgctc
     2581 ggagaaagac tccgcagaaa tcatggatga attgaaggga gtctacttcg acgtcgcgca
     2641 cgcggcctat ccggcgccac tcgccgcgat cacgaaagta attccgat
//
LOCUS       DASZMB010000049         4777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3352060, whole genome shotgun sequence.
ACCESSION   DASZMB010000049 DASZMB010000000
VERSION     DASZMB010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4777
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3352060"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VG272_02095"
     CDS             <1..550
                     /locus_tag="VG272_02095"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF12499.1"
                     /translation="GKLLGHPNFAPILEELNHRMAVIFVHPTISCCAMPIHHLNRVQI
                     DFPTDTTRTITDLIFSGSLIRFPDIRWIFSHGGGTVLMLTPRLAGGNLTPEERAVTIP
                     NGFEHELQKLYYDIASVALSPIPMAAVLKGIPKEHLLFGSDIPFFTIERIAAAVNKFE
                     VPGADLRLIQRENALQLLPRLR"
     gene            complement(636..1577)
                     /locus_tag="VG272_02100"
     CDS             complement(636..1577)
                     /locus_tag="VG272_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWF12500.1"
                     /translation="MSIRNKWSGAKIAASFVFAGALLMPAASSAQLKQPLLSEDNLKR
                     VSDHVYAIEGFPNVAIIVGSRATMVVDTGLGQKNGATVMRAEQKLAKGPILYLTTTHY
                     HSEHTTGEQAFPTSTIIIRNQAQQDEMDKSVAGHIEVFKKMSAQNMELLQDVKFRAPD
                     VVFDRETKVDLGGVTARLFWLGPAHTKGDELIFVEEDSVLIPGDIVQKDIFPIMPNPD
                     ASVKGWLSILDNVEALHPKFIVPDHGPAVVDASQIQTQRAYLLALQSRALELKKQGVS
                     VDDAGKTVTTELKAKHPDWPNPNNVAGEVSRVYDQNP"
     gene            complement(1616..1963)
                     /locus_tag="VG272_02105"
     CDS             complement(1616..1963)
                     /locus_tag="VG272_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12501.1"
                     /translation="MSLTFIDTNKIPHSKISGSGEFAEVLNNQICGAKNVVGSLRWLN
                     TGEKLEVRSDKDTHQLVYLMEGEGAITMDSKDHSVRKGAGVYVGPSDSVTIKHAGAAP
                     LKLFHLVVPHLPA"
     gene            complement(2062..2469)
                     /locus_tag="VG272_02110"
     CDS             complement(2062..2469)
                     /locus_tag="VG272_02110"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWF12502.1"
                     /translation="MAYIFNEDELPRLLSNGRERVFFVNKELTKIDDMLAGIMHYKKD
                     ASSPYHMHKNCEHFYFIMDGKATVQSEEGTREIGPGMMVFIPAEEKHRLRALEPLFYF
                     EFQAPNRFQTTILEGTPDDLKWDRVDGGIWMQS"
     gene            complement(2795..4453)
                     /locus_tag="VG272_02115"
     CDS             complement(2795..4453)
                     /locus_tag="VG272_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWF12503.1"
                     /translation="MSKRYLRFRVSEVRSIAIIFLFLLALALFVGGAQQFGGRPLAAG
                     PAAADDTDWTYADHDLNGTRYSPLKQITAGNVKQLAKVCSYTFPEKVPSESEPIVSAG
                     VIYATSDHYTVALDAADCHVLWSYQWVPRATDRVHPHRGAAIANGKIIRGTGDDFLIS
                     LDAETGKLQWAQQIANPDEGHFIAMPPLVHGDLIYIGPAGSERAGTGWIGAFKLSTGE
                     QVWKFNVIPQDGEPGADTWGPNPEARKHAGGALWTAQSYDTEKDVLYIAGGNPAPDFF
                     DDARPGINLYTNSIIALDGKTGKLLWYNQFIPHDVDDYDITHVNPIFKVNSRTMIASS
                     GKDGVLRVVDGETHKVIYSSPFTTQLNSEVPLTHTPMKVCPGILGGNEWNSGGFSPNL
                     TLIVMPSNDLWCSMNMKDNEAPSVAKANTGEKSYFGGPIDHGAFSGSRGRLTGFDAAT
                     GKERWRYESPTPMVAGVAITAADLIFTGETGGFFDAVDAQTGKVLLHYDMGDSIQGGV
                     VTYSAHNMQYVAVTSGDGNVLGNKNFPEIKGGNPTVTVFGLPKK"
BASE COUNT         1013 a         1348 c         1260 g         1156 t
ORIGIN      
        1 tggaaaatta cttggtcatc cgaatttcgc gcccatactg gaggaactca accaccgcat
       61 ggccgtaatt tttgtgcatc cgacgatttc gtgctgcgcg atgccgatcc accacttgaa
      121 ccgcgtgcag atagatttcc cgacggatac gacacgcacc atcacggatc tgatttttag
      181 cggctcgctg attcgctttc ctgacattcg ctggattttt tctcacggcg ggggaacggt
      241 gctaatgctg acgccgcggc ttgccggcgg caatctgacg ccggaggagc gcgcggtgac
      301 cattccaaac gggttcgagc acgaactgca gaagctttac tatgacatcg cgagcgttgc
      361 gctcagccct attcctatgg ctgccgtgct caagggcata cctaaggagc atctcctgtt
      421 cggctcggac attccgtttt ttacaattga acggattgcg gcagcggtga acaaattcga
      481 agtgccgggc gccgatttgc gattgattca gcgggagaac gcgctgcaac tgcttccacg
      541 gctcaggtag gacaggcttc agcctgtctt cttttgaatt ttgatgatgt tacgacgatc
      601 aaaaccagac aggctgaagc ctgtcgtgct taaatctacg gattttgatc gtacacgcgc
      661 gagacttcgc cggccacgtt gttcgggttc ggccaatcgg ggtgcttggc tttcaattcc
      721 gtagtgaccg ttttgcccgc atcgtcgacg gaaactccct gttttttaag ttcaagggcg
      781 cgcgattgca gcgccagcag gtacgcgcgc tgcgtctgaa tctgcgaagc gtccacgacg
      841 gccggcccat gatccggaac gatgaatttc ggatgaagcg cttccacgtt atccagaatc
      901 gagagccagc ccttcacgct tgcgtccgga ttgggcatga tggggaaaat atctttctga
      961 acgatgtcgc cggggatcag aacgctgtct tcttccacaa aaattaattc gtcgcccttc
     1021 gtgtgcgcgg gccccaacca aaagagtcgc gccgtgacgc cgcccagatc aacctttgtt
     1081 tcacggtcaa acacgacatc aggcgcgcga aacttcacgt cctgcagcaa ctccatattc
     1141 tgcgctgaca tttttttaaa gacttcaata tggccggcca cagacttgtc catttcatcc
     1201 tgctgcgctt ggttgcgaat aataatcgtg ctcgtgggaa atgcctgctc gccggtggta
     1261 tgttccgagt ggtaatgcgt tgtggtgaga tacaggatcg ggccttttgc cagcttttgt
     1321 tccgcgcgca tcacagttgc gccattcttc tgccctaacc cggtatcgac gaccatcgtc
     1381 gcgcgactcc cgacgatgat cgccacgttg gggaaacctt caatggcgta gacgtgatcg
     1441 gagacgcgct tcaggttgtc ttcagaaagc agaggctgct taagctgcgc actcgatgct
     1501 gcgggcatca gcagcgcccc tgcgaaaacg aacgatgcgg cgatttttgc gcctgaccac
     1561 ttatttctta tcgacatccg cggtctccct ttttttcaga aaaacgccgg ctatctcagg
     1621 ccggaaggtg cggcacgacc aaatgaaaaa gtttcagcgg ggctgcgccc gcatgcttga
     1681 tcgttacgga atcggacggg ccgacgtaga cgccggcgcc tttgcgcaca ctgtgatctt
     1741 tggagtccat cgtaatggcg ccttcgccct ccatcaggta gaccaactgg tgcgtgtctt
     1801 tgtcggagcg gacctccaac ttttctccgg tgttgagcca gcgcaatgaa ccaaccacat
     1861 tttttgcgcc gcagatctgg ttgttcaaaa cttccgcaaa ttctcccgaa ccagagatct
     1921 tactgtgcgg aattttgttt gtatcgataa atgtcagtga cattggggac cgagtccttt
     1981 cgaggccatt taatttcccg ttccaagctt cgagcgcatt cgaattgcag atttcaattc
     2041 tttgcggcgt ggatcgcgcg attagctctg catccagatc ccgccgtcga cgcgatccca
     2101 cttcagatcg tctggcgtcc cttccagaat cgtcgtctga aaacggttag gggcctgaaa
     2161 ttcaaaataa aaaagtggct ccaacgcgcg cagtcggtgt ttttcttccg cgggaatgaa
     2221 aaccatcatg ccgggaccga tctcgcgggt tccttcttca gactgcacgg tggctttgcc
     2281 atccatgatg aaataaaaat gttcgcaatt tttgtgcatg tgatacggag acgatgcgtc
     2341 ctttttgtag tgcatgattc ccgccagcat atcgtcaatc tttgtcagtt ctttattgac
     2401 gaagaacacc cgctcgcgcc cgttagataa cagcctgggc agttcgtctt cgttgaaaat
     2461 gtatgccatt actgggcctc ccgttcagat gtgctgctgg ctgcgcggca aactccgtaa
     2521 ccaactctac gtttcttatc cgccgtgggg cgattgaatg cgcacatgct atcagttttt
     2581 ttgtgatgct gccagaacgg tcggaatttt gtggagggtt gagcgcagac gacgcagtga
     2641 acatttaaat cgaaattcaa agtcaaaagc cgcccgccca aaaccggccg tccgctacga
     2701 aaccgccgcc cggtttatgg aacgaagtcg gcagcctcag atgcccggca gattccgccc
     2761 gtgcgcggaa ccgcgcactt tcactaagca actatcactt tttaggtagc ccgaaaacag
     2821 tcaccgtcgg atttccacct ttgatttccg gaaagttttt atttcccagg acattgccat
     2881 cgcccgaagt aaccgccacg tactgcatat tatgcgcgga gtacgtgacc acaccgcctt
     2941 gaatcgagtc tcccatatcg tagtgcagaa gaacttttcc cgtttgcgcg tcaaccgcgt
     3001 caaaaaatcc gccggtctct ccggtaaaaa tcaggtcggc cgcggtgatc gccactcccg
     3061 cgaccatcgg cgtaggcgat tcatagcgcc agcgctcttt tccagtggcc gcgtcgaagc
     3121 cggtcagccg gccgcgcgat ccgctgaatg cgccgtgatc aatcggcccg ccgaaataac
     3181 ttttctctcc ggtattcgct ttcgcgacgc tcggcgcttc attgtccttc atgttcatcg
     3241 agcaccatag atcgttggac ggcataacga tcagggtcag gttcggactg aacccgccgc
     3301 tgttccactc gttgccgccc aaaattccgg ggcacacttt catcggtgta tgcgtcagcg
     3361 gcacttccga atttagttgc gtcgtgaacg gagaactgta aatcactttg tgcgtctcgc
     3421 cgtcgaccac gcgcagcacg ccgtccttgc cggaggacgc gatcatcgtg cgcgagttca
     3481 ctttgaaaat cggattgacg tgcgtaatgt catagtcatc gacatcgtgc ggaataaact
     3541 ggttgtacca cagaagcttc ccggtctttc cgtccagcgc gattatcgaa ttcgtgtaga
     3601 ggttaattcc cggccttgca tcgtcaaaga aatcgggcgc aggatttccg cccgcaatgt
     3661 agaggacgtc tttttcggtg tcgtaggact gcgccgtcca taacgccccg cccgcgtgtt
     3721 tgcgcgcttc cggattgggt ccccacgtgt cggcgccggg ttcgccatcc tgcggaatca
     3781 cattgaattt ccacacttgc tccccagtgc ttagcttgaa tgcgccaatc caccccgtgc
     3841 ctgcgcgctc ggagcccgcc ggtccgatat aaatcaggtc gccgtgaacc agcggtggca
     3901 tcgctataaa atggccttcg tcagggttgg caatttgctg cgcccactga agttttccgg
     3961 tttcggcatc cagcgaaatt aaaaaatcat cgcccgtgcc gcggataatt tttccgttgg
     4021 cgatcgcagc gccgcgatgc ggatgaacgc ggtcagtcgc gcgcggaacc cactgatagg
     4081 accacagcac gtggcaatcg gcggcgtcga gcgccacggt gtaatggtcg ctcgtcgcgt
     4141 aaatcacgcc cgcggaaacg attggctccg actccgatgg aaccttttcc ggaaatgtgt
     4201 aggagcatac ctttgcgagt tgcttcacgt tgccggcggt gatctgcttc agcgggctgt
     4261 aacgcgtccc atttaaatcg tggtccgcgt aggtccagtc cgtatcgtca gcggcggcgg
     4321 gaccggcggc aagcggtcgg ccgccgaatt gttgcgcgcc gccaacgaaa agagccagcg
     4381 ccagaagaaa caagaaaatt atcgcgatcg accggacttc agagactcgg aaccgcaaat
     4441 atcgcttact cattttgttt tccctctatc tgaaaatggg atcatcgctc aatgcgctga
     4501 ggccactttg tttccaccgc accggtttgt caagcattga ccgtgtggcg tccggggact
     4561 ccgccgccgc ctccataatt tgaaatttgg aaatcggcgc atataatgcc tcaggtcttc
     4621 ttcaacaaaa tggactccta atcaggaatc tggacaggaa agggtgatat tgatggaaga
     4681 ggaaaaggaa ccgacgattt ccgattcaaa aacgctggtc gaccggcgcg attttatagg
     4741 aggtgcaacg atgctcgcag gatcctcact tctagga
//
LOCUS       DASZMB010000050         7422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4183850, whole genome shotgun sequence.
ACCESSION   DASZMB010000050 DASZMB010000000
VERSION     DASZMB010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7422
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4183850"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VG272_02120"
     CDS             <1..202
                     /locus_tag="VG272_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12504.1"
                     /translation="LHYSEITIKSTFHHTPRFIREALESVSSGEIRSSDFVNAEIPLT
                     ELPKMFEHMKHRNGEMKVAVIP"
     gene            290..2242
                     /gene="hpnC"
                     /locus_tag="VG272_02125"
     CDS             290..2242
                     /gene="hpnC"
                     /locus_tag="VG272_02125"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HWF12505.1"
                     /translation="MQTSAELEIGRGLPPSGCSVAEAERYTRRLATGHYENFNVASWL
                     LPRHLHQHFYNLYAYCRWADDLGDEIPDSGRAIALLNEWDQELRGCYAGEASHPVFIA
                     LHKTISELNIPIEPFSDLLIAFRQDQTVHRYVTWSDVYGYCRYSANPVGRLVLYLCGY
                     RDAERQRLSDATCTALQLANFWQDVTRDLEKGRIYIPMEALAAHGLMEDDIVQRRFDA
                     RYVSLMKELIARTRQLFSEGLPLASSVDSKLRVDIEMFSRGGMSVLDAIEAIGYNTLE
                     RRPSISKSKQFTLLLRALSLRLISFVKPEAPVSLPNSANFDNPGKLEIVEWNIAKADA
                     PSNRPKNENEVVHSYTECRRVARVSASNFYYAFFMLPAAKRDALCALYAFMRLVDDVS
                     DTVGSAADKQRGLARWRAALDAAVSGDVSGHPILPAFADTIERFKIPPRYFHDLISGA
                     EMDLTIGDYPTFDRLREYCYRVAGTVGLTCLHVFGFTDPHAPDLAEKLGIAFQLTNMI
                     RDVRADYEMGRIYLPLEDLNQFGVRPETLSPGPATLEIRELLEFETARAWSFYSEGVN
                     LIPLVDTDSRAALWALVRIYSSLLRRIETRGYDVFSERVRLTKAEKTRILLRARLGWY
                     SQNNVLEERDRDRRRPGGTFLGRRAG"
     gene            2172..3596
                     /gene="hpnE"
                     /locus_tag="VG272_02130"
     CDS             2172..3596
                     /gene="hpnE"
                     /locus_tag="VG272_02130"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HWF12506.1"
                     /translation="MSSKSVIVIGGGLAGLSSAVALAEAGFRVRLLEKRPHLGGRAAS
                     YVLPGGEHVDNCQHVTLGCCTNLEDFYRRVGAANQIRFFGRLMFATPDGKRGVISAAA
                     LPPPLHMAFSFAFYPLLGLADKFAIGQALLAIAFSGGKPNDLASNVADENITMLAWLK
                     KHRQTDRAIRRFWEVILVSALDEDLDRIDARYGIDVFWKAFLSTRAGYRLGIPRVPLS
                     DLYEGCRRSLAAQGGEVQLRAGVRGFQVANGHVSGVEREDGSVEMADYYVAAVPQDVL
                     PDLLPAEIVEREPAFYNLKNLRASPITGVHLWFDRTVMTEPFLTLLDSTTQWVFNKTQ
                     LYGSGAENGGQYLQLVISASYNLTSRSRQEIIAMCLEELRAVLPATREATLLKGTVVK
                     EMSATFSPSPGSDRWRPGRGTSLTGLFLAGDWTSTGWPSTMEGAVRSGYLAAEAILSD
                     AGTPRKLLQPDLRVEGLARLWARE"
     gene            3725..4183
                     /locus_tag="VG272_02135"
     CDS             3725..4183
                     /locus_tag="VG272_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12507.1"
                     /translation="MDRKYKHRGYQSVDEPQKKDKRPHEGHPRQEQIGPRTPRMVGTI
                     TRARCSNCGAVLQPGFDPKGKCPRCAFELHCCKQCAYFDTAARFECMKPISEQIARKD
                     QRNECTFYEFRTTIEKDTAPSAPSAGSAASPERVSRPLDARQAFENLFKK"
     gene            4375..4722
                     /locus_tag="VG272_02140"
     CDS             4375..4722
                     /locus_tag="VG272_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12508.1"
                     /translation="MKLTIRNAHEEWREPVEKETNRQAAKLEKLLVRYDPDLVQLHAN
                     IEKHARKESYTFTVTVSLPTGTLHATGEGTDVLASVKVSFAEIITQCKKHQSLLRKDY
                     EWKRKRPHVRITA"
     gene            4841..4912
                     /locus_tag="VG272_02145"
     tRNA            4841..4912
                     /locus_tag="VG272_02145"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4873..4875,aa:Lys,seq:ttt)
     gene            6087..6632
                     /locus_tag="VG272_02150"
     CDS             6087..6632
                     /locus_tag="VG272_02150"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWF12509.1"
                     /translation="MSVLETERLVLRKVDQEDLKDIIAWEALPGVQNTEVEAQEFLEY
                     CFREYRERGIGPWGMQLKATGAIVGNCGFPHIAFKKRCGEVNYDVTLRHRGEGLAPEA
                     LRALLRFGFLDIGLIRIQARCDIDNLSSERVMLKAGMRFEGLIEDSPLSKDPNPKQKL
                     YAISANDFNRATDRQRIWLAD"
BASE COUNT         1733 a         1906 c         1984 g         1799 t
ORIGIN      
        1 gctgcattat tcggagatca cgatcaagtc cacgtttcat cacacacctc gcttcattcg
       61 cgaagcgctg gaaagcgtgt cgagcggaga aatccgctcc agcgatttcg tgaacgcgga
      121 aatcccgctc accgagcttc ctaaaatgtt tgagcacatg aagcatcgca acggcgaaat
      181 gaaagtcgcg gtgattccgt aacgcctttt ctgtagcatg aacttcagct tgtgcgtttt
      241 attcttgcaa gattaaaagc tcacaggctg aagcctatgc cactgcccca tgcaaacatc
      301 tgctgaactt gagatagggc gcgggcttcc gccgtctgga tgctcggttg cggaagcgga
      361 gcgctacacg cgccgcctcg ccacgggcca ttacgaaaat ttcaatgttg cttcctggtt
      421 gcttccccgc catctgcatc agcattttta taatttatac gcatactgcc gctgggccga
      481 cgatctggga gatgaaattc ccgattccgg gagagccatt gcgctgctga atgaatggga
      541 ccaggaattg cgcggttgct acgcgggaga agcatcgcat cctgttttca tcgcgcttca
      601 caaaacaatt tccgaattga atattcccat cgagccattt tccgatctgc tcattgcgtt
      661 tcgccaggac cagaccgtgc atcgctacgt gacctggagc gatgtgtacg gctactgccg
      721 ctattccgca aatccggtgg gacgtttggt gctgtattta tgcgggtatc gcgatgcgga
      781 gcgccagcgg ctctccgacg caacctgcac ggcgctgcaa ctcgcgaatt tctggcagga
      841 cgttacgcgc gatctggaga agggccgcat ctacattccg atggaggctc tcgcagcgca
      901 cggcctcatg gaagatgaca ttgtgcagcg gcggtttgac gcgcgatacg tttcactgat
      961 gaaagaattg atcgcacgga cgcggcaatt gttttcggaa ggattaccgc ttgcgagtag
     1021 cgttgattcc aaactgcgcg tggatattga gatgttcagc cgtggcggga tgtcggtact
     1081 cgatgccatc gaggcgattg gatacaatac gctcgagcgc aggccctcga tttcaaaatc
     1141 gaagcagttt acattgctgt tacgtgcgct ctccctgcgg ttaatttcct tcgtaaagcc
     1201 ggaagcgccg gtttcacttc ccaattcagc caattttgac aatcctggga aattagaaat
     1261 tgttgagtgg aacatcgcga aggcggatgc gccctccaat cgtccaaaaa acgagaacga
     1321 agtcgtacat tcgtacacgg agtgccgccg cgtggcgcgc gtatcggcca gcaattttta
     1381 ctacgcattt ttcatgctgc cggcagcgaa gcgcgatgcg ttgtgcgcgc tctacgcgtt
     1441 tatgcggctt gtcgatgacg tatcggacac cgttggcagc gccgcggata agcagcgggg
     1501 actggcgcga tggcgcgcgg ctctggacgc ggctgtcagc ggcgacgtct cgggccatcc
     1561 cattcttcct gcgttcgcgg acaccatcga acgcttcaaa attccgccgc gctattttca
     1621 tgatttaatt tccggcgcgg aaatggactt aacgattggc gactatccga cgttcgatcg
     1681 tctgcgtgaa tactgctatc gcgtggctgg gaccgtgggg ctcacttgcc tgcatgtctt
     1741 cggttttacc gatccgcatg cgccggacct ggccgaaaaa ctgggcatcg cttttcagtt
     1801 gacgaatatg attcgcgatg tgcgcgcgga ttacgaaatg ggccggattt atttgcccct
     1861 cgaagactta aatcaattcg gagtccgccc cgagactctt tctccgggac cggcgacgct
     1921 ggaaattcgc gagttgctgg aatttgaaac cgcgcgcgcg tggagctttt acagcgaagg
     1981 cgtcaacctg attccgcttg tggatacgga ttcgcgcgcc gcgctttggg cgctcgtgcg
     2041 aatttacagt agcctgctgc gccgtattga gacgcgcggg tatgatgtat tctccgaacg
     2101 cgtgcggctg accaaagccg aaaaaacaag gatcctgctg cgtgcgcggc taggctggta
     2161 ctcccaaaat aatgtcctcg aagagcgtga tcgtgatcgg cggcggcctg gcgggacttt
     2221 cctcggccgt cgcgctggct gaagccggtt tccgcgtgcg tttgctcgaa aagcgtcccc
     2281 acctgggcgg tcgcgctgcg tcctatgtgt tgccgggagg agagcatgta gataactgcc
     2341 agcacgtgac gctcggatgc tgcacgaatc tggaagattt ttatcgccgc gtcggcgctg
     2401 caaatcaaat ccgatttttc ggtcgcctga tgtttgccac tccggacgga aagcgcggag
     2461 tgatttctgc cgctgcgttg ccgccgccgc tgcacatggc attttcgttt gcgttttatc
     2521 cgttgcttgg tctcgcggac aaatttgcga tcggtcaagc attgcttgcg atcgcattca
     2581 gcggtggcaa acccaacgat cttgcgagca atgttgcgga tgaaaatatc acgatgctgg
     2641 catggctaaa aaagcatcgt cagaccgatc gcgcgattcg ccgcttctgg gaagtgattc
     2701 tggtcagcgc gctcgacgaa gatttggatc gcatcgacgc gcggtacgga atcgatgtgt
     2761 tttggaaagc gtttctctcc acgcgagcgg gctatcgcct tggaattccg cgtgttcctc
     2821 tcagcgattt gtacgaaggc tgccgcaggt ctcttgccgc gcagggcgga gaagttcagt
     2881 tgcgtgcggg cgtgcgcgga tttcaagtgg caaatggcca cgtgagtgga gtggaaaggg
     2941 aagacggctc ggtggagatg gcggactatt atgtcgctgc cgttccgcag gatgttttgc
     3001 cggacttgct gcccgcggaa atcgttgagc gcgagcccgc attttacaat ttgaagaatt
     3061 tgcgcgcatc gccaatcacg ggagtgcatc tatggtttga tcgcacagtg atgaccgaac
     3121 cgtttttgac tttactcgat agcacgacgc aatgggtttt taataaaaca cagctgtatg
     3181 gcagcggcgc ggaaaatggc ggtcaatatt tgcagcttgt gatcagcgcg tcctacaacc
     3241 tgacttcacg ctctcgccag gaaattattg cgatgtgcct tgaagaattg cgcgcagtgt
     3301 tgcccgcgac ccgggaggcc acactgttga agggaactgt agtgaaggaa atgtcagcca
     3361 cgttttcgcc ctcgcccggt tcggaccggt ggcgcccggg gcggggaaca tcccttacag
     3421 gattatttct agcaggggac tggacatcca caggctggcc gtccacaatg gagggagccg
     3481 tacgtagcgg ttacttggcc gccgaagcga ttctctccga tgccggaacg ccacggaaat
     3541 tattgcaacc ggatttacgc gtagaaggat tggcccgcct gtgggcccgc gagtagcaaa
     3601 aagtaacgca cttaggattt ttcgacccgc aaacagaagt attaattttg tagacaggtc
     3661 tgcttaacac taagcttgca tgttttaatt cggaatattt ttgagcattc ccccggaggt
     3721 tgccttggac cggaagtata aacatagggg ctaccagagt gtcgacgagc cccagaagaa
     3781 agataaacgt ccacacgaag gccacccgcg acaggaacag atcgggccgc gaacgccgcg
     3841 catggttggc acgatcactc gcgcccgctg ttcgaattgc ggcgccgtgc tgcagccggg
     3901 cttcgatccg aaagggaagt gcccacgttg tgcattcgaa ctgcattgct gcaagcagtg
     3961 cgcgtatttt gacaccgccg ctcgcttcga atgcatgaaa cccatctccg aacaaattgc
     4021 gagaaaagat cagcgcaatg aatgcacgtt ttacgaattt cgaacaacca tagagaagga
     4081 tactgcgccg tctgcccctt cggcgggctc cgctgcatcg ccagaacgcg tttcgcgtcc
     4141 tttggatgcg cgccaggcgt ttgaaaacct ctttaagaaa taaccaccca gaatcaactg
     4201 gcagtttcaa agtttcaatt tgatccgcaa attgatttct gtgtgcgcgt tcaggaacat
     4261 gccgtttgca accgcatcgg cgaattgtta ttctgaaatt ggtcatgcat ttttggatta
     4321 aaccccgagt cacaatctcc atccttaata aaataattcc cggaaaggcc tttcatgaaa
     4381 ttgacgattc gcaatgctca tgaggaatgg cgcgagcccg tcgaaaagga aaccaatcga
     4441 caagctgcga agctggagaa attgctggtg cggtatgatc ctgatctggt gcagttgcat
     4501 gccaatatcg aaaagcatgc gcgaaaagaa agttacacat ttaccgtcac agtctcgctt
     4561 ccgacaggta ctttgcatgc cacaggagaa ggcaccgatg ttctggccag cgtgaaagta
     4621 tcttttgccg aaatcattac gcaatgcaaa aagcatcaat cccttttgcg caaagactac
     4681 gagtggaaac gaaagcgtcc acacgtccgc ataacggcct gatatttttt caaattttag
     4741 aatggagtga gtgattccga atcgctctaa ttttacgatg aagtgtcgtt ctctgttgca
     4801 gcatgagcat acgatctata caatcaatgg tttgtacagc aggggcgtag ctcagtggta
     4861 gagcagcggc cttttaagcc gaaggtcgtg ggtccgatcc ccaccgcccc tactaagtct
     4921 aatcaactga aagttcatca gttaatcctt ccgtctttcg cgtgtacctc agacgtaccc
     4981 ttaccttcca acaatacgac tggatcttcg ctccatgctt tttgagatcg gcctctagtt
     5041 gttcctgcct agaccgcgtc caaggagcgt agtgtcgctc ggtcactcgg atactttggt
     5101 gcccgagaag aactgacacg cgctcaagag gaacgccggc aagcaatagc tccactgcaa
     5161 acgtatcgcg gtaacggtga gcatggccat tcgaaacctt cgcgagttcg aagattcttt
     5221 gtaaccgccg ctgcccggat cctgaccgtt gttagtactt actctcgcgt aaagcgccgt
     5281 tttcattgtt ttggctccca aaatgcccgc gctcgggtga ctttaaccgg cctaggaccg
     5341 gccgaggtca ccccagattt ggccgattgc agcctattag cgtggcgttc gggaccgcgg
     5401 ggcaagttag ggcataaaac gtatatacga ggcctgcggc tccctaacac actattatac
     5461 ctggatgaga caataggtaa atcatagttt aggtatatct gccttgacag tactgcggcg
     5521 caccccttag gatagccgta tgattatctg cacactgaaa caggtattaa ggtgtaaggg
     5581 ctggacacgt tataggcttg aaaaggaatc aggcgttacg tttccaacgc tgtacgcgat
     5641 gttctacgga aagagcaaag gctatagtgc cgaagtattg aatcgactat gtgcgacact
     5701 gcactgtaag ccgggagact tgttaaggtg ggaaccggac agattctccc gcccgaaacc
     5761 acacgctaag tagatgtact tccatggact cgctctacat tttgctacgc gcatcaatct
     5821 gagtatttac gctgccatga ccgggacgcc agtcttttgc tccttgccgc catcatttta
     5881 cttgcacttc gtatccatgt catattggca tactttgaaa tgaaaatagg aaagaggacg
     5941 atgtcaactc gttcacaatt atcggacact cacgaatggc tccaagagat tcaataaata
     6001 tcttttcttg ttggtcgctt atcaaggcta tgcggaaccg tattcgtcga attctgcccg
     6061 tggagtctgg atatcgacag ttaaagatgt cagtgcttga gactgaacgc ctcgtgttgc
     6121 gtaaggttga tcaagaggat ttaaaagaca taatcgcttg ggaagcactc cccggtgtac
     6181 aaaacactga agttgaagca caggaatttc ttgagtattg ttttcgggag taccgtgaac
     6241 ggggcatcgg tccgtggggc atgcaattga aggcgaccgg ggcaattgtg gggaactgtg
     6301 gttttccaca catcgctttc aaaaaacgct gtggagaggt caattacgac gtcactcttc
     6361 gacatcgtgg agaaggattg gctcccgagg cgctgagagc cctcttgaga ttcggatttc
     6421 tggatatcgg actgattcga atccaagccc gatgcgacat agacaatttg agttccgagc
     6481 gagtaatgct gaaagcagga atgaggttcg aaggactgat agaggactct ccgctttcga
     6541 aagacccgaa tccgaaacaa aaactctatg cgatctcagc gaacgatttc aaccgggcga
     6601 ccgaccgcca gcgtatatgg ctggcagatt aacggagcag cgcggtctgg tagacgttga
     6661 gaaatcctcc gaaacatccc ccacaggtga cctgattctg tcgaccgagg gtcgcgactc
     6721 tcatctttcg agattgccag accaattaaa tgaaaactac ttctggccct cgcatcggta
     6781 agaacgaaat ctatccttgt attccactaa tcctcagaat tgaattttac acagggtgaa
     6841 tttttgattg cgctcgattt tggcttgaca tacatatatt ttggctatat ctagttgttc
     6901 ccttaatcaa gtgacaaact gccttatcaa ttgttcttct cgggtctaat cggtctcggg
     6961 ccatgaagat gccgaagcat tccatagcgc gagcactagc cgctttgcca ctcagcttcg
     7021 aacctaatgt agggcaaacg gacccttcag tccagtttgt tccgcaaagc tctggttaca
     7081 cagtttttct tacttctaat gaggctatga tctcacaaca gggacccgac ggtttcctcg
     7141 actcaagaag cagcacaaat aatccagtct gagcttagtt caatagtcgg cggattggcg
     7201 cacagtagaa agcgaacgaa aggcgatctc cctcaatttc tagattgccg tgcaactcgt
     7261 ctgcgtcaaa gaacaacaag tcaccgggcc gcaattggat cgcgattccc cagcgaggaa
     7321 tcaccaggtg gccaccttaa aaggccattg atccggttcc ggtgcgttaa ttttcggcgg
     7381 cacggatgac aaccggattt ctgagcacct tcaaattggc cg
//
LOCUS       DASZMB010000051        10910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4720069, whole genome shotgun sequence.
ACCESSION   DASZMB010000051 DASZMB010000000
VERSION     DASZMB010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 10910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10910
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4720069"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="VG272_02155"
     CDS             complement(<1..1238)
                     /locus_tag="VG272_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12510.1"
                     /translation="MSKKKCIVALGLAAGLCLLVLLNARSARTQGGPKYKVDAEWPKP
                     LPNKWKMGGVTGLAVDKDDNVWVHDRPNDLTDIELEAELTPPIADCCARPPSMIHIDK
                     EGNVIGSIDPPQGHGMTVDSKGFIYLGNVVNGIGNVRKYDPKTGAQLAELPHTPEAPP
                     AGPGVDAAVDSPRPGHGSMGPVFGFLPGGRPKPDPAARAAREVATAAFRAKYPPTTPM
                     IVGGIEEVRLDEPAHELYAADNYLGGRVMVFDMDTFHFKRGWGAYGHKLSEITTDDKD
                     RAYVPNGPMPKEFRGHLTLNVSHDGLVYAADRMANRIDVTTKDGTFVKEFIMAPWTGV
                     GGSTGGVMFSPDKEQRFLFISDLTNNHIWFLNREDGKVISTLGSMGQNAGQFYGLHMI
                     SVDSKGNIYTGEVFGGERVQR"
     gene            complement(1515..2720)
                     /locus_tag="VG272_02160"
     CDS             complement(1515..2720)
                     /locus_tag="VG272_02160"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HWF12511.1"
                     /translation="MSSETGNHILGFSTAGRIFFGWGAASVLRDVAKEFGPRVFICTD
                     RNMVKAGVCETVVAQLSEGKAGIKIFEGGQPEVDRQTIERAAAEAQAYKPDVVIGLGG
                     GSNLDLSKAVALLVKYPGQISSYYGENNVPGPITPVVAVPTTAGTGSEVSPVAVVADP
                     ERAMKIGIASRTLIPKWAIVDPALTVSCPASVTAHSGMDALVHAIESYCARVRTDCSP
                     HAIFVGKNPVSDVLATKAIIAITEFLPIAVAQPAHREAREQMALASLLAGMAFSAAGT
                     AAVHALQYPVGEATHTPHGLGNAVLLSTVMKRITPSRVKEMAFIARSLDSSLVAKTDA
                     EAAAQAATLVDQFAAKVGIPKGLRTLGLKESQLPELAKMASTVKRLLDNSPVSFNESE
                     LLLLLQEAY"
     gene            complement(2710..3750)
                     /locus_tag="VG272_02165"
     CDS             complement(2710..3750)
                     /locus_tag="VG272_02165"
                     /inference="COORDINATES: protein motif:HMM:NF014653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HWF12512.1"
                     /translation="MTIPQETSIVVSAEDVRATIFHVVSRLGASEREASIQADVLTEA
                     DLRGHHSHGLQRLPVLASRIKKQLIRVNVAPDYLWTADSVLSVDGKDGLGPFVAETAH
                     EHAIPVLRQKGIVAVAIRNSSHIGMIGYYCERRAREGFVCIAMTESEALVHPHGGTNA
                     LVGTNPIAIGIPSEPSPFVFDMATSTSAIGKIYASKHRGESIPLGWAIDAEGKPTTNP
                     DAALKGSLTPLAGPKGYGLGISIGILSGLLTGAEIGRAVLGTLDTEFRCTKGDLFLLL
                     NPAAFPGGRELAAQVGGYLDEIRSSPPQQDCEPVIVPGDRARKVREESTRSGIPLPKE
                     VWLAAERLKNEF"
     gene            complement(3795..4406)
                     /locus_tag="VG272_02170"
     CDS             complement(3795..4406)
                     /locus_tag="VG272_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HWF12513.1"
                     /translation="MADRKLNAKKTALIIQDLQNDVITEGGAFASSGAPAHAKKQNVV
                     ENVKNLAAAMRKAGGVVIHVHYIVEQGAPGLKLNAPLFRGLAEGKAVVRGTWGAAPAE
                     GLEPQKGDMLVEKMRMNGFHGTKLDTILQGFGIESLVITGAWTNFSIEHTARHGADAG
                     YDVVVATDGTSTINDEWQNAGINYALQNIADRWSCQEIIQALS"
     gene            complement(4415..5338)
                     /locus_tag="VG272_02175"
     CDS             complement(4415..5338)
                     /locus_tag="VG272_02175"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HWF12514.1"
                     /translation="MNESASTVEVKSSELLNLIDKNATVEQLCTGFWFTEGPIWNPKE
                     QFLYFSDMPGDVRRRWSAADGVKEVRRPANKCNGMTYDGAGNLYVCEHVTSSLIMETP
                     SGERKVLASHWQGKELNSPNDVVVRSDNSVYFSDPTYGRMPVFGLERKQELNFQGLYR
                     ISPDGKLHLEADDFEQTNGVCFSPGEKLLYVNDSPRAHIRVFDVGTDGSLSNSRIFAE
                     KIGDGILENGIVDGMKVDERGNVYVTGPRGIWVFSPAAEHLGVIRMPEHAGNLTFGGR
                     NWDELYCACSKSIYRVKMKVRGNPVAYMNIR"
     gene            5757..5939
                     /locus_tag="VG272_02180"
     CDS             5757..5939
                     /locus_tag="VG272_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12515.1"
                     /translation="MKAAGSYEEIQPIEHNPEMQADGEEFYPKGAIAFFVSMLIGFGI
                     IWGALYMVMVHRALQL"
     gene            5963..6769
                     /locus_tag="VG272_02185"
     CDS             5963..6769
                     /locus_tag="VG272_02185"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWF12516.1"
                     /translation="MSKSSKNKTGVNGIEKRVILTALLVSCLGMGLIAYSTWGMGITI
                     ADCVPNSKIFERGSVKKLAEKNYEIHFLASMWAFEPSRVTVPAGSSLDLFVISKDVTH
                     GFNIIGTNVNMMVEPAVVNEARVHFEHPGTYTIVCHEYCGVGHQAMNAVIEVSDSAQD
                     ISADGLPASESSASASTLPGKAIMDQKGCLACHSVDGTVSVGPSFKGLWGTKAELTDG
                     TSPTVDAAYVKDMIESPGKKVVKGFPPVMPKLPVSDEELGQIIEYLKALR"
     gene            6773..8452
                     /locus_tag="VG272_02190"
     CDS             6773..8452
                     /locus_tag="VG272_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HWF12517.1"
                     /translation="MEQQKALRAIIVAELLVPLLLLIFGIYHGVLQVMYRAGVIHGSS
                     VAGIDYYQGLTAHGVINALVLTTLFAVALGHAMVAQELELPISVGGAALSLAVMLIGA
                     VMAAAMIFAGKATVLYTFYPPLKASPFFYIGLSLFIVGSWIPLYTWIPPYLRWRKANP
                     GKKTPLAVVGIFTTFIVWQIATLPVAFEVLVLLVPWSMGWTPGVNVVLSRTLFWFFGH
                     PLVYFWLLPTYTLYYTMLPRLAGGKLYSDFAARFSFFAFIVLSSPLGLHHQFADPGIS
                     SNWKGLHTVLTLLVVVPSLVTAFTLSASLEHGARSKGGAGLFGWWRKLPHFDREKWLF
                     PYLFLGLVIFIFGGITGIVNASYAMNGVIHNTAWLPAHFHLTVGGPVFLAILGMSLYL
                     ITGLLNKRVAMPALAIAVPYIWMTGTFLFSGGLFAGGLRGEPRRTNLGLTYLNPQSPS
                     FRPDWQITTHFALIGGCIMALAVLVYFAVLISSLVGAREYSAAPFSMPMSEPLHDEDI
                     AAVRNFTPWVAAAVLAVVLAYYPPLNDIMKSHFKPVPGYRPDSPIALKAGE"
     gene            8452..9180
                     /locus_tag="VG272_02195"
     CDS             8452..9180
                     /locus_tag="VG272_02195"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HWF12518.1"
                     /translation="MLLKGFMRRGRIVLLLALLAAARADARIVPATQTRLPVPDVQVL
                     DASDNSASIRSIIATMGTGPIVVLPVYTRCAASCPLQTQKIKQVWKDLNQASSIRILV
                     FSFDPQETAASISDYRRREGVPDSWVVVHARESDARSFFDFFQYSVMSDNGQFVHPDR
                     IFLLDSASHWRFTMEGLKYSPEEIEHALAQLQSPGLGVWIQTNPDAVAWTGFLCILVT
                     LCVLGIWRIGHKSPKPSFVAKNQA"
     gene            complement(9426..10499)
                     /locus_tag="VG272_02200"
     CDS             complement(9426..10499)
                     /locus_tag="VG272_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF12519.1"
                     /translation="MDHNKPMIAILFTLITLLGAKYTAAEPMNWNKTVSAAKAAQEQG
                     QYAQAEALYSQAVAMQAKTLGANSPVVAVSLNNLASAYQAQSMNNQAEQAYTQAMTIL
                     SKAPRQEESLARTMNNLAALYVDMGADQKAEALYTRALGIWQKLPKKDNANQAISNIG
                     LAGIYHSRNDDAAAEPFYLRALSNWNAENKADSADAADAMLHLADYYRFRSNTDAAAV
                     MYAHVVGIWEMSGQINTAQGIAAQSALGEIYRAEGKYEQAEPLVERAVKATEAKLGSN
                     DLQLAASLNNLALIYYCEGKYSAADPLYKRALQIRESNLGASDPAVQHTAEIYAALLR
                     LEGKRADAQKVENFSARAIAPHF"
BASE COUNT         2259 a         3207 c         2856 g         2588 t
ORIGIN      
        1 cgctgaaccc gctctccgcc aaatacttct cccgtataaa tgtttccttt gctgtccact
       61 gaaatcatat gcaaaccgta aaactgacca gcgttctgac ccatgctgcc cagtgtgctg
      121 atgactttgc cgtcctcacg attgagaaac cagatgtgat tgttcgtcag gtcggaaatg
      181 aacaggaatc gctgctcttt atcaggcgaa aacatcacgc cgcccgtcga gccgccaacg
      241 cctgtccacg gcgccatgat gaattccttc acgaacgttc catccttggt ggtcacgtca
      301 atgcgattgg ccattcgatc tgccgcgtat acgagcccat cgtgcgaaac attcagcgtc
      361 aggtgtccac gaaattcctt gggcattggt ccgttcggaa cgtacgcgcg atctttgtca
      421 tccgtcgtga tttccgaaag tttgtggccg taggcgcccc agccgcgctt gaagtggaac
      481 gtatccatgt caaacaccat cacccgcccg ccgagatagt tgtccgccgc atacaattca
      541 tgcgccggct catccaggcg aacttcttca atcccgccaa cgatcatcgg agttgtcgga
      601 ggatatttcg cgcgaaacgc ggccgtcgct acttcacgag cggcacgtgc cgcgggatct
      661 ggtttagggc gccctcctgg cagaaaccca aagacgggtc ccatgcttcc gtggcccggt
      721 cgtgggctat cgaccgcggc atctacgccc ggaccggcag gaggcgcctc gggcgtgtgt
      781 ggcaattccg caagttgcgc tccggttttc ggatcgtatt tgcggacgtt accaattcca
      841 ttcaccacgt tgcccagata gataaatcct ttgctgtcga ccgtcatccc atgcccctgc
      901 ggtggatcga tcgacccaat gacgttgcct tccttatcga tatgaatcat cgacggcggc
      961 cgcgcgcagc aatccgcaat cgggggagtg agttccgctt ccaattcaat gtcggtcaaa
     1021 tcattcgggc gatcatgcac ccagacattg tcgtctttat cgacggcgag tcccgtgact
     1081 ccgcccatct tccatttatt gggtagcggt tttggccact ccgcatccac tttatatttc
     1141 ggtccaccct gcgtgcgcgc gcttcgcgca tttaacaaca ccaacaggca cagcccagcc
     1201 gccaagccca gtgccacaat gcattttttc ttactcatcg aattcccccc tcattacacg
     1261 atcagatttt gcaggctgac catctcggtg agaatgctta tacacccgac ccacatttcc
     1321 gtaaacctgc gaaatcggcg accgcggcgg gaagtttata tcgctgtagc tcgcctcttc
     1381 agaggcgggg gtttaggcgt cacgcgtcga ccatgccttg cggagaatct aaatctcggt
     1441 ctctgaagag gcgagcgaca gcggtgcggt ccatcgtcga gggcgcccgc gaggtcagaa
     1501 cgcttcgaga agattcagta cgcttcctgc aaaagcagca gcagctcgct ttcattgaat
     1561 gaaaccggac tattgtccag cagccttttc acggtggatg ccattttcgc gagctcggga
     1621 agttgcgatt ccttcagccc cagggtacgc agcccttttg gaattcccac ctttgccgcg
     1681 aactggtcga cgagcgttgc cgcctgcgcc gcggcttccg catccgtttt tgcgacgagg
     1741 ctcgaatcga gactgcgcgc gataaacgcc atttccttga cgcgagaagg cgtaatgcgc
     1801 ttcatcactg tagaaagcag gaccgcattt cccagcccgt gcggcgtatg cgtagcttcg
     1861 cccacgggat attgcagcgc atgcaccgcg gcggtacccg cagcggagaa tgccataccc
     1921 gcgagcaagc tagcgagagc catctgctct cgcgcttcac gatgagcagg ctgcgcaacc
     1981 gcgattggca gaaattctgt gatcgcgata atcgccttcg tcgccagaac atcggaaaca
     2041 ggatttttgc ccacaaaaat tgcatgagga gagcaatcgg tacgtacgcg ggcgcaatat
     2101 gattcaatcg cgtgcaccag cgcatccata ccggaatgcg ccgtgacgct tgccggacac
     2161 gagacggtca gcgcaggatc gacaatcgcc cacttcggaa taagcgtgcg cgacgcgatt
     2221 ccaattttca tcgcgcgttc cggatctgcc accaccgcga ccggactgac ttccgatccg
     2281 gtgcccgccg tggtgggaac cgcaaccacg ggcgtaatcg gccccggcac attattctcg
     2341 ccataatagg aagaaatttg cccaggatac ttcacgagca gcgcgacagc tttcgacaga
     2401 tcgagattgc ttccgccacc caggccgatc accacatcag gcttgtacgc ttgagcttcc
     2461 gcagcggcac gctcaatcgt ctggcgatcg acttccggtt gaccgccttc aaaaatttta
     2521 attccagcct tgccctcaga aagctgcgcg acaacggttt cgcacacgcc cgctttcacc
     2581 atgttgcggt ccgtgcaaat aaaaaccctc ggcccaaatt ccttcgccac gtcgcgtaac
     2641 acgctcgccg cgccccaacc aaagaaaatt cttcctgccg ttgaaaaccc cagaatgtga
     2701 ttgccggttt cagaactcat tttttagcct ttctgccgcg agccaaactt cttttggaag
     2761 agggatgccc gaacgcgtac tctcttcgcg tacttttctt gcacgatctc ccggcacgat
     2821 caccggttcg caatcttgtt gcggaggcga gctgcggatt tcgtcgagat acccgccaac
     2881 ttgcgcggcg agctctcgtc cgcctggaaa cgccgcggga ttcagcagca aaaacagatc
     2941 tcccttggtg cagcgaaact cggtgtccaa agtgccgagc accgcgcgcc caatttccgc
     3001 gcccgtcaaa agtcctgaca gaattccgat cgaaattccc aagccgtagc ctttgggtcc
     3061 cgccagtggc gtcagcgaac cctttagcgc ggcgtccgga tttgtagtgg gcttgccttc
     3121 cgcatcgatc gcccagccca gcggaatact ttcgccgcga tgtttactgg cataaatctt
     3181 cccgatggcg ctcgtcgaag tggccatatc gaaaacaaac ggagagggct cgctcggaat
     3241 tccgatagcg atcggattcg tgccgaccag cgcattcgtt ccgccgtgcg gatgaaccag
     3301 cgcttcgctc tccgtcatcg cgatgcacac aaagccttcg cgcgctcgtc tttcgcaata
     3361 atatccgatc atcccgatgt ggctgctgtt acgaatcgct accgcgacga ttcctttttg
     3421 ccgcagcacc ggaatcgcgt gctcgtgcgc agtctccgca acgaacggtc ccagtccatc
     3481 ttttccgtcc acgctcagca cggaatccgc ggtccacagg taatcgggag cgacatttac
     3541 acggatcagt tgtttcttga tgcgcgacgc aagaacgggg agtcgctgca acccatgcga
     3601 gtggtgcccg cgaagatccg cctccgtcag cacatctgcc tgaatggaag cttcgcgttc
     3661 actcgcgcca agccttgaca ccacgtgaaa gattgtcgcg cgcacatctt ctgcagaaac
     3721 gacgatggat gtttcctgag gaatagtcat gcactctcga tggggaacaa gctgtccgag
     3781 ccaacctgtc tagattaact caaagcctgg atgatttcct ggcaactcca gcggtcggca
     3841 atattctgaa gggcataatt aattcccgca ttttgccact cgtcgttaat cgtggaggtt
     3901 ccgtcggtgg ccaccaccac atcatacccg gcgtccgcgc cgtgcctcgc ggtgtgttcg
     3961 atggaaaaat tcgtccaagc cccggtgatc accagcgatt cgattccgaa gccttgcaaa
     4021 atcgtgtcga gtttcgtgcc atgaaacccg ttcatgcgca ttttctcaac gagcatgtcg
     4081 cccttttgcg gctcgaggcc ttccgctggc gccgcgcccc acgttccgcg caccaccgct
     4141 ttgccttcgg ccaaaccgcg aaacagcggc gcgttcagct ttagtccggg cgcgccctgc
     4201 tccacaatat agtgaacgtg gataaccact ccgccggcct tgcgcattgc agccgccaga
     4261 ttttttacgt tctccaccac gttctgcttc ttcgcgtgcg caggcgcgcc ggaagaagcg
     4321 aatgcgccgc cttcggtaat cacatcattc tgtaaatcct gaatgatcag cgccgtcttt
     4381 ttagcattga gttttcggtc ggccattttc cctcttagcg tatattcatg tatgcgacag
     4441 gattcccgcg aactttcatt ttcacgcgat aaatcgattt cgagcatgcg cagtacaact
     4501 catcccaatt gcgtccgccg aacgtcagat tcccggcgtg ctccggcatt cgaatgacgc
     4561 cgaggtgttc cgccgcggga ctgaacaccc agattccgcg cgggcccgtg acgtaaacat
     4621 ttccgcgctc atcgaccttc atgccatcga caattccgtt ttccagaatg ccgtcaccga
     4681 ttttctctgc gaaaatgcgg ctgttgctga gcgacccatc cgtaccgaca tcgaacacac
     4741 ggatgtgtgc gcgaggggaa tcattcacgt acagaagttt ttctccgggc gaaaagcaaa
     4801 cgccatttgt ctgttcaaaa tcgtccgctt cgagatgcag cttcccatcc ggcgaaattc
     4861 tgtaaagtcc ctgaaagttg agctcctgtt tgcgttccag gccgaagacc ggcatgcggc
     4921 cgtacgtagg atcgctgaag taaaccgaat tgtcggagcg aacgaccacg tcattcgggc
     4981 tgttcagttc ttttccctgc cagtgggagg ccaacacttt ccgctcgccg gacggagttt
     5041 ccatgatcaa cgagctcgtc acgtgctcgc acacatacaa atttccggcc ccgtcatagg
     5101 tcatgccgtt acatttattc gcgggccgac gcacttcttt cacgccatcc gcagcgctcc
     5161 agcgccggcg cacatcgccc ggcatgtcgc tgaaatacag gaactgctct ttcggattcc
     5221 agatgggccc ttcggtaaac cagaagcctg tgcaaagctg ctcgaccgtg gcatttttgt
     5281 cgatcaggtt gagcaattcg gaagatttaa cttctaccgt gctcgcggat tcgttcattt
     5341 cgttttaccc ctcaaagcgt cagtcatccg atttgatttt cactcacgga attctttgta
     5401 gtcacgcagt aggtacgcta atttctcgtc aaatgttaag cagactatat gcacgccacc
     5461 aactcgtgtc aaacgaacat agccaagtgg cgcgggcttt agcctgcgga atttagtcct
     5521 tgcaaggccg aaccccggca ggctgaagcc cgcgccaaca aaacgtacgg tccttccgca
     5581 agcctcttaa ctcatcgttc tcgccacttg cagcacaatg atttgctcca acgtggcaca
     5641 gcccacattt aagtgtgatg gacatcacag aacagaaaat ccttaatctc tattatcgtg
     5701 gctgtcacac acaacccgat ctcgtgcgcg caggtctgcg cccttgtgga gaaactatga
     5761 aagccgcagg cagctacgaa gaaatacaac cgatagagca caatcccgaa atgcaggccg
     5821 acggcgaaga attttatccc aagggcgcta ttgcattttt cgtcagcatg ctgattggct
     5881 ttggcatcat ctggggcgct ctttacatgg tgatggtgca ccgtgctctt cagctttaaa
     5941 aattcaaagg cgggtcctga ggatgtcgaa aagttcaaaa aataaaacgg gcgttaacgg
     6001 tatcgaaaag cgcgtgattc tgacggcgct cctggtttcg tgcctgggca tgggattgat
     6061 cgcatactcg acatggggta tgggcataac gattgccgat tgcgtgccca actcaaaaat
     6121 tttcgagcgt gggtcggtca agaagcttgc cgaaaaaaac tacgagattc atttcctcgc
     6181 gtccatgtgg gcattcgaac cgagccgcgt tacggttcca gcgggctcgt cgctcgatct
     6241 ttttgtgatc agcaaagacg tcacgcatgg cttcaacatc attgggacca acgtaaacat
     6301 gatggtcgag cccgccgtgg tgaacgaagc gcgcgtccat tttgaacatc ccggaaccta
     6361 caccatcgtc tgtcacgaat attgcggcgt cggccatcag gccatgaacg cggtcatcga
     6421 agtgagcgac agcgcacagg atatttctgc agacggcttg ccagcctcgg aatcctccgc
     6481 atccgccagc acgcttccgg gaaaagctat catggatcaa aagggttgcc tcgcttgcca
     6541 cagcgtggat ggcacagtca gcgtaggccc cagcttcaaa ggcctgtggg gcacaaaggc
     6601 agaattgacc gacggcacct cgccaacggt ggacgcagcg tatgtcaagg acatgattga
     6661 atctcccgga aagaaagtcg tgaagggttt ccccccggtc atgcccaagc ttccggtgtc
     6721 ggatgaggaa ctcgggcaaa tcatcgaata cctaaaggcg ttgagataaa ccatggaaca
     6781 acaaaaagcg ttgcgagcca ttattgttgc ggagttgctg gtgccgctgc tgctattaat
     6841 tttcggaatt tatcacggcg tgctgcaggt gatgtaccgc gctggcgtaa ttcacggttc
     6901 gtccgtcgcc gggatcgatt actatcaggg ccttacggcg cacggagtga ttaatgcgct
     6961 ggtcctcacg accctcttcg ccgtggcctt gggccacgca atggtcgcgc aggaattgga
     7021 gctccccatc tccgtgggcg gcgccgcact ttctctcgcc gtgatgttga tcggagccgt
     7081 gatggcggcg gccatgattt ttgccggcaa agcgaccgtc ctttacacct tttacccgcc
     7141 gctaaaggcc agcccatttt tctacattgg cctgtccctt tttatcgtgg gatcgtggat
     7201 cccgctttac acctggatac cgccctatct tcgctggcgc aaagcgaatc cgggcaaaaa
     7261 aactccgctg gcggtcgtag gaattttcac aacttttatc gtctggcaaa ttgcaactct
     7321 gccagtcgcg tttgaggttc tggtgcttct cgtgccgtgg tctatgggtt ggactcccgg
     7381 cgtcaacgtc gtcctgagcc gcacattatt ttggttcttc ggccatccgc tcgtctattt
     7441 ctggctgctg cccacgtaca cgttgtacta cacgatgctt ccgcgccttg ccggcggaaa
     7501 actctatagc gatttcgccg cgagattttc ttttttcgcg ttcattgttc tgtcgtcgcc
     7561 actcggtttg catcatcaat ttgccgatcc cggaatttcc agcaattgga agggcctgca
     7621 cacggtgctg acgttgctgg ttgtggtgcc cagcctcgtc accgccttca cgctctctgc
     7681 ttctctcgag catggcgcga gaagtaaagg cggcgcggga ctattcggct ggtggagaaa
     7741 gcttccgcat ttcgaccgcg aaaaatggct ctttccttac ctttttctag gcctggtcat
     7801 ttttattttt ggcggcatca ctggcatcgt caacgcttcg tacgcgatga acggcgtgat
     7861 ccacaacacg gcgtggctcc ctgcgcactt ccacctcacg gtaggaggac ctgtttttct
     7921 cgccatcctc ggcatgagcc tctacctgat caccgggctt ctgaacaagc gcgtcgccat
     7981 gcccgccttg gctattgccg ttccgtacat ctggatgaca ggaacgttcc tgttttccgg
     8041 agggcttttc gccggaggtt tgcgcgggga acctcgtcgt acgaatctgg gcctcactta
     8101 cttgaatccg caatcgccca gcttcaggcc ggattggcaa atcaccacgc attttgcgtt
     8161 gatcggcggc tgcattatgg ctctggccgt gctggtttat tttgccgtgc tgatctcgag
     8221 cctggtcggc gcgcgagaat actcagcagc cccgtttagc atgcccatga gcgaaccgct
     8281 gcatgacgaa gacatcgcag ccgttcgcaa ctttacgcct tgggtagccg cagccgttct
     8341 agcggtagtt ctggcttatt atccgcctct gaacgacatc atgaagagtc atttcaaacc
     8401 cgttccaggc tatcgtcccg atagtccgat cgcgctgaag gctggagaat aatgctgctg
     8461 aagggtttca tgcgccgcgg acgaatcgtt cttctcttgg cgctgttggc cgcagcgcgt
     8521 gcggacgcgc gcatagttcc tgcaacgcaa acacgtttgc ccgttcccga tgtgcaagtt
     8581 ctggatgcgt ccgataattc cgcctccatt cgttcaataa ttgccacgat gggaacaggc
     8641 ccaatcgtcg tcctgcctgt ctacacgcgg tgtgcggcca gctgtccgtt gcagacgcaa
     8701 aaaatcaagc aagtttggaa ggatttgaat caggcatcgt ccattcgcat cctcgttttt
     8761 tcctttgatc cgcaggaaac agcggcaagt atttccgact atcgccgtcg cgaaggagtg
     8821 cccgatagct gggtcgtcgt ccacgcacgc gaaagcgacg cgcgatcgtt ctttgatttt
     8881 tttcagtatt cagtgatgag cgacaacggg caatttgtcc atccagaccg catttttctg
     8941 ctggattccg cttctcactg gcgattcacg atggaagggt taaaatacag tcccgaagaa
     9001 atcgagcacg ccctagcgca acttcaatcg ccgggcttgg gcgtatggat ccaaacgaat
     9061 cccgacgccg tggcatggac gggctttctg tgcatccttg tcaccctctg tgtgttaggc
     9121 atctggcgaa taggccacaa atcgcccaag ccatcctttg tcgccaaaaa tcaagcgtaa
     9181 gaataccgcg agaactacat gtaaaagctt gctaccttcg gaatgatgat cagcacgcga
     9241 gcagcacagc cactcttggc tgtgtgcaat ttgcaaaacc cgcacatgca ggagtgcctg
     9301 tgctactcaa agattcgtcc aaaccatttt tcagtcgtgg ctcattctaa ggcgggatat
     9361 tgcgaggcgg ctgcaaggag gaggcaaccg cggcccgctg cgaagggcgg gccgcggccg
     9421 aggagttaaa agtggggagc gatagcgcgg gcggaaaaat tttcaacttt ttgagcatcg
     9481 gctcgtttgc cttccaggcg aagcagcgcg gcgtaaattt ccgcggtgtg ttgaacggcc
     9541 gggtcgcttg cgcccagatt gctttcgcga atctgtagcg cccgtttgta gagagggtcg
     9601 gcggctgagt atttaccttc gcaataataa ataagcgcaa ggttattcag gctggctgcg
     9661 agttgcaggt cattcgagcc aagcttcgcc tcggtggcct tcactgctcg ttcgaccaga
     9721 ggttcggctt gctcgtactt tccttccgcg cgataaattt caccaagtgc actctgtgcc
     9781 gcgattcctt gcgccgtgtt aatctgtcct gacatttccc aaattcctac cacgtgcgca
     9841 tacataacgg ctgctgcgtc ggtattgctt cggaaccggt agtagtcagc caaatgaagc
     9901 attgcgtccg cggcgtccgc ggaatccgct ttgttttcag cattccagtt cgacagggcc
     9961 cgcaggtaaa aaggctccgc tgctgcatcg tcgtttcggg agtgatagat tcccgccagt
    10021 ccgatgttgc tgatggcttg gttggcattg tctttcttgg ggagcttctg ccaaattccc
    10081 aaagctcgcg tgtagagtgc ctcggctttc tgatccgcgc ccatatctac atagagggct
    10141 gccagattat tcatcgttcg cgccagcgac tcttcctggc gaggagcctt gctcaggatt
    10201 gtcatcgctt gcgtgtatgc ctgctcggct tggttgttca tggattgcgc ctggtaggca
    10261 gacgccaggt tgttcaggct gaccgcgacg acggggctat tcgcgccgag cgtttttgcc
    10321 tgcatggcca cggcttggct atacaacgcc tccgcttggg catactggcc ctgctcttgg
    10381 gctgccttgg ccgctgaaac cgtcttattc cagttcatgg gctctgcggc cgtgtatttc
    10441 gcgcccagaa gcgtgatcag cgtgaacagt atggctatca ttggcttgtt gtgatccatc
    10501 gaatgcctcc tcgtttggtg gtgcgctttc gttttcgcaa gacaactgtg ccaaatcgag
    10561 gcggcgacac ctatgaggca tcagtgccaa tttgtgcaga caaagggttt ggtagtacca
    10621 ggaccgggtg ctggacaccc cggcgtccgg gagcaaactt cgcgatcatt cattaactcg
    10681 ttttattgca gatagttgca cgcggcaccc gggcctgcgt gggtcgagcc caaggcctcg
    10741 aatgcagcgc aaactttagt tttttcgacg gaatttgctt agaattgaga ccggattagc
    10801 ctaccgcgcc taaatctcgc gcagccgaaa gagtcgaacg cggctgaaag ccgcctggct
    10861 ggagccgccg caacagcgcc attcgcagtg acgcaaggtg cacatctagc
//
LOCUS       DASZMB010000052        14967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2532262, whole genome shotgun sequence.
ACCESSION   DASZMB010000052 DASZMB010000000
VERSION     DASZMB010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 14967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14967
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2532262"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VG272_02205"
     CDS             <1..293
                     /locus_tag="VG272_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12520.1"
                     /translation="GSYVIHNIGNGVALNLQYHITRGIPEFDQPKIRTRRYMPAIPAT
                     ARVTLVETIGSYNAEHDATFEYESIGKRKYRSSMKLNHHVITAFNFEEITHQ"
     gene            601..1941
                     /locus_tag="VG272_02210"
     CDS             601..1941
                     /locus_tag="VG272_02210"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HWF12521.1"
                     /translation="MNPTKPDTQVAAEGITRMSRDTLSITDNRTGKSYEVPIKDETIR
                     ATDLRQIKVNGDDFGMMSYDPAFNNTALCQSKITFIDGDKGILRYRGYSIEELAEKST
                     YLETAYLILHGELPTRAQLDDWTYHITHHTFIHESVKKFLDGFHYDAHPMGMMISAIA
                     ALSTFYPEAKDIFNKESRKKQTYRLIGKMPTLAAFSYRHSLGMPFAYPDNELSYPGNF
                     LNMLFKTSELKYRPNPTLERALDVLFILHADHEQNCSANAMRCVGSSHVDPFSATAAA
                     TAALYGPLHGGANEEVLRMLLEIGSLANVPAFIKRVKAGETKLMGFGHRVYKNYDPRA
                     RIIKHVADGVFEIMGRNPLLNIALECERIALEDDYFVSRKLYPNVDFYTGLIYQSMGF
                     PVTMFPVLFAIPRTSGWIAQWEEMLLDPEQKIARPRQIYTGYDLRPYASLDQRK"
     gene            complement(2062..2559)
                     /locus_tag="VG272_02215"
     CDS             complement(2062..2559)
                     /locus_tag="VG272_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HWF12522.1"
                     /translation="MTQLFLLQLPVQRMRQFVAPVLRGIALMILGALIAQSARAQKIT
                     MEFDQTADFSKYKTFAIREGQLNSRNPALGGELVKKQIEVDIQNGLTAKGLTMVTSGP
                     ADLNVRYTFGAARKSEVEAYPAGWYGMGTRYVRVPYAEGTLVMDLPARSDDPLARLAL
                     DCRGR"
     gene            2675..3409
                     /locus_tag="VG272_02220"
     CDS             2675..3409
                     /locus_tag="VG272_02220"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HWF12523.1"
                     /translation="MQKPFFAPQSFPIYNGFMRNTGIHFDEFATARRAMVETQIRKRG
                     VSSERVLEAMATVPRHEFIPEQFRNDAYADKPLPIGEGQTISQPYMVAAMTEALELKG
                     SERVLEIGTGSGYQAAVLSTLVSQVITVESQTSLALAAQERLTKLGYTNVHVHNGDGS
                     AGLPDAAPYDAILVTAGAPQIPQVFANQLREGGRIVIPVGDRENQDLVCAQKENGELK
                     SHVLFGCKFVLLLGRYGWNRVERKFS"
     gene            complement(3431..3940)
                     /locus_tag="VG272_02225"
     CDS             complement(3431..3940)
                     /locus_tag="VG272_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWF12524.1"
                     /translation="MTPRREYPEQPMVGVGGVVVHEGRALLIRRGSAPLKGEWSIPGG
                     LLEVGETLEDGVVRELAEETGLDVRVIELIEVFERIFPAPPNTDGTPGDPARPQYHFV
                     ILDYLCEIRGGTIAAASDALEFAWAREEDLMKFDVTVAVKRVLRKAFARAQERDLTPE
                     SRTSPTNEI"
     gene            4064..4303
                     /locus_tag="VG272_02230"
     CDS             4064..4303
                     /locus_tag="VG272_02230"
                     /inference="COORDINATES: protein motif:HMM:NF044773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12525.1"
                     /translation="MVRCPKCSTEIDVDEDEVEEGEILACPECETEMEVVQIHPVKVN
                     AISEDDDEEEDEESEDGDDLEDEDDEDDDEEEEDI"
     gene            4359..4763
                     /locus_tag="VG272_02235"
     CDS             4359..4763
                     /locus_tag="VG272_02235"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWF12526.1"
                     /translation="MRLTIAFVGLALLSGTTSAFEQPGGPQSSSKSHAHDFVIFTTVF
                     TEQGFALSGARVRVRRSNEKKFRWEAMSDRRGELAFRVPQDSEYEMTVEARGYKTLTR
                     KVDGTEGNRADLTIRMEPLSEPHTDSGTGGKS"
     gene            4760..5131
                     /locus_tag="VG272_02240"
     CDS             4760..5131
                     /locus_tag="VG272_02240"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWF12527.1"
                     /translation="MNLRGTLATIALIGLAATVMFAQGNPKGKESQLRTVRGTVVDNT
                     ETPSETAVVYLKNTRTQDVITHLSDSDGTFRFSGLDLNVDYDIHAEKDGWTSASRSIS
                     NFDTRKEFVLTLKLDRKKGGK"
     gene            5433..5978
                     /locus_tag="VG272_02245"
     CDS             5433..5978
                     /locus_tag="VG272_02245"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWF12528.1"
                     /translation="MAIQFFVNGKPQSVDVPPNMPLLWVLRDTLGMTGTKFGCGMALC
                     GACTVHIEGEATRSCITPISSVAGKKVTTIEGISPDRSHPVQRAWIEVDVPQCGYCQS
                     GQIMSAVALLAKNPKPNDTEIDEAMRGNICRCGTYQRIREAVHHAADLHKASATPASF
                     DGAGATLEARNQNAVEAGGAA"
     gene            complement(6238..6990)
                     /locus_tag="VG272_02250"
     CDS             complement(6238..6990)
                     /locus_tag="VG272_02250"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HWF12529.1"
                     /translation="MNPKWQGGSRHIAILHAFQRHSRATISWYLYSAAMNAKNKIVIE
                     HIFFDWDGTLLNSFEADANAYAYMFGALGMDWSIAQLKRHYSPNWHLVYRAAGIPRAK
                     YGEADRLWRRFYQKQLPKLQPGAMNVLRILDRRFKLALVSSGSRTRVRKQLRQHNVSA
                     MFLTKVCCEDAPRRKPHPAPLRTALEKLGARPHTSVYIGDAPEDIEMAHRAGVRAIGV
                     LGGSPVPERLRAASADALIETIRDLPALLKNF"
     gene            complement(7018..7542)
                     /locus_tag="VG272_02255"
     CDS             complement(7018..7542)
                     /locus_tag="VG272_02255"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWF12530.1"
                     /translation="MRTSRIQKLTSQSDPSRGNRGIEELSASSATVVADIPSWITRAK
                     PKGISRVSTHAVRPPEERRAYSRANLSLALRVKRIAGQREKKMEPLRTTNISSSGVLF
                     LCPYRIAPGTPVEIEICLVERPLGRGSVKMLTEAHVVRADFAMRPGWHMLAATFDDIT
                     FQREEPSPSRFQRH"
     gene            7797..8891
                     /gene="ada"
                     /locus_tag="VG272_02260"
     CDS             7797..8891
                     /gene="ada"
                     /locus_tag="VG272_02260"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HWF12531.1"
                     /translation="MATRSYPGMQALPPVARKKYLAAVASHDARMDGAFVYAVRSTGI
                     YCRPSCPSRRPHENQILFFNKPEEAEQAGFRACLRCHPRENRNSSKVQLVRKVCKQIE
                     SRPDGSVSLKNLAEHTGLSPAHLQRTFREAMGITPRQYAEALRVTHLKSELRKGSNVT
                     TALHEVSFGSTSRLYEKSNAQLGMTPATYRKGGQGMNISYTIADSSMGRVLVAGTERG
                     ISAVYLGDKDSDLAASLHEEYPQAEIQTIPEGKSKWVRAIVAHLDRSNSPLDLPTDVA
                     GTAFQRRVWQALREIPLGATRTYSDVARAIGQPTAIRAVGHACATNPASIVVPCHRVI
                     RTDGTLGGYRWGLHRKQSLLEKERRAAKAK"
     gene            complement(9074..9793)
                     /locus_tag="VG272_02265"
     CDS             complement(9074..9793)
                     /locus_tag="VG272_02265"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HWF12532.1"
                     /translation="MKLPTAIYRLCVSFCAAALAAVFFFTPLLSATPQSSWTLDNVMK
                     QMDAQAADFRSLTADMERTKVTVVVNDKSTESGRISVRRDDKMLIEITQPDARTILRN
                     GDIFDIYNPKIHRVEEYNIGKKKSLVDQFLLLGFGTSGSSLKESYTVAVQGEETLDGH
                     KVVRLELLPKTDEVRKQLSKIQLWLDESTWLPVQQQFFETGSGDYFIIHYKNMIRNAR
                     IADSEFKPHWPHGTTVVQPQA"
     gene            9998..10876
                     /gene="folP"
                     /locus_tag="VG272_02270"
     CDS             9998..10876
                     /gene="folP"
                     /locus_tag="VG272_02270"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWF12533.1"
                     /translation="MFHRKRFRLRLRSRALILGERTLIMGVLNITPDSFSDGGAFLDS
                     EAAIAHGLQLEKDGADILDIGGESTRPGAAPIKPEEEIRRILQVIQILSGKLGIPISV
                     DTRRAEVAKQALDAGAEIVNDVSGLRSDAQLANVARLARAPLILMHMRGTPQTMQRGP
                     FSRNIIRDVRSELRDSMARAKKAGISKTRILLDPGIGFGKTFEQNFELLARLPEFARI
                     GCPIVVGTSRKSFLGKALADSGGASVPAEERLLGTAATMTASILGGAHIVRVHDVAEM
                     VRVARVADHIVNATRS"
     gene            10990..12093
                     /locus_tag="VG272_02275"
     CDS             10990..12093
                     /locus_tag="VG272_02275"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HWF12534.1"
                     /translation="MKFNKMLPALLAILLSPCLPAGAQLTPAEPDPLARIRDAAAANV
                     QACSATGETLCEQVAPKIIANAQGDSPLAENLRLLRKNLATRNKFVQEGRAVEWAMAA
                     FRETGLDPHIEKYANPNGSAGEQQNVVAEYRGRDNSNEWVLVGAHLAPRSNGSSAVEN
                     ICDAASVIEAARDIALTAMHPKRSIRFVLFTGDERDMLGSWAYVRSHRAELDHARAAI
                     IFHSNCYRVSGYSLNGGLDIEAGLRDAMKPIESLGATHFVTGAKTGTDDFDFLLEGVP
                     TLSAYPILMPVSVDSPMLLPNSGKTELADLKHNVAVVAVTAFGIAERTEPIGPRQSHA
                     EIELLLETFGLEFQMKTAGTWKQWESGERGRLP"
     gene            12147..12665
                     /gene="ruvX"
                     /locus_tag="VG272_02280"
     CDS             12147..12665
                     /gene="ruvX"
                     /locus_tag="VG272_02280"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HWF12535.1"
                     /translation="MAVPIPLRALSLAADPPTPGVILAIDYGKKRLGLALSDEFGVTS
                     RPFATWTRINRRRDLSRLRDLVRQEKIRRIVVGLPLRLDGTPSEMSEEAKSFALRVEK
                     AIGLPVEMVDERLSSWEARETVSQMNSNKRPRRSSDRGGSSKKTPIDDIAAAIILRDY
                     LDRTQTRPGSRG"
     gene            12667..13728
                     /gene="mltG"
                     /locus_tag="VG272_02285"
     CDS             12667..13728
                     /gene="mltG"
                     /locus_tag="VG272_02285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HWF12536.1"
                     /translation="MRRALLYLLILAILAVGYICVALYVPYQGFGSDGVYVDIPHGAS
                     QRTISRLLAENGVVRNRFAFELLARLRKRRTMQAGEYFFNRPVTAFDVYDTIVNGRVF
                     VKEFVIPEGFTMFDIADLAAQEGFLTRDEFLAAARDPSSISDLAPDAPSLEGFLFPAT
                     YEFPRHMMGKEMTSAMTRKFRQEWLALSDAAATTHPNVQSVVTLASLVERETPRPEER
                     PHVAGVFENRLRIGQPLQCDPTVAYAMTLAGAFSGKLSGGDLRFSSAYNTYENRGLPP
                     GPIANPGEAALRAAMDPPPTDDLYFVANTEGGHFFSKTLQEHNHNVARYRHLLEESEK
                     PSAGSAPPGPAKNKSKPAG"
     gene            13734..14297
                     /locus_tag="VG272_02290"
     CDS             13734..14297
                     /locus_tag="VG272_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12537.1"
                     /translation="MARGASPTKKKVILEIAGELAVPRFTPAEVEQIRRQLVARLGAA
                     GRTSAEYIAGVLETAGMRIVWSTKADTEGHYEEEFHNLLHFANLADAELCIMRLDELY
                     RKFHQEKEHAATERVLEVARLGRRRAEMIARNHKVEPEKRAEKEEIMQWFKVWLETPT
                     AFFDWLEARKATPDFKKRFAKSDSADA"
     gene            14302..14871
                     /locus_tag="VG272_02295"
     CDS             14302..14871
                     /locus_tag="VG272_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12538.1"
                     /translation="MIKDPIYVLEVEPLDLGADLKAVGLDLVEIESREPVRGVDAAKL
                     WAAALTALPGAEPWGFDFFAHLDRVREYCRMHGVAFEEKTSGNSIVIPSPMGDALAAL
                     VERFAGETFGIRAGALFAAGDSAVEGELAKHGVDGYHSAFRNYLFCAVCDFENGFLTL
                     LTEKLWTSEVLRRIKPATAGMPVEVARPQ"
BASE COUNT         3449 a         4057 c         4072 g         3389 t
ORIGIN      
        1 agggcagtta tgtcattcac aatatcggaa acggcgtggc gctgaatcta caatatcaca
       61 tcactagggg cattccggag ttcgaccagc caaaaatacg aacgagaaga tatatgcccg
      121 cgatccctgc tacggctcgc gtcacattgg tcgaaaccat tggaagctac aatgccgagc
      181 atgatgccac tttcgagtac gagagcattg gcaaaaggaa atatcgctca tccatgaagc
      241 tcaatcatca tgtaattacg gcattcaatt tcgaggaaat cacgcaccaa taacgcctgt
      301 ccccgaaagt ccacaggggc gggcgggtgg cagggccttt gaagcgggtt cgaatttccg
      361 ggtggcccac gcttccggtt gaagcgtggg gctttttgca cttggctttc gttcctctga
      421 tttgatgaat cgctcagcga tctcgcggct tccggcggtt tgcgcgcctc actggggacg
      481 ctcagcgcaa atacagggcc tgcgaccatt ccttgtggat tttggcgcga taacactccc
      541 gaaaaatgca aagttagtgt aaattcctct gtggcagttt ttcagaaaca cggaaacgcc
      601 atgaatccaa ctaaacctga tacgcaggta gcagcagaag ggattacccg catgagccgg
      661 gatactcttt ccatcacaga taaccgaacg gggaaatctt acgaagttcc catcaaggat
      721 gaaacgatcc gcgccaccga tcttcgccag atcaaagtaa atggcgatga tttcggaatg
      781 atgagttatg acccggcttt taataatacg gcgctgtgtc agagcaagat cacgttcatt
      841 gacggcgaca aaggcatcct gcgctatcgc ggctattcga tcgaagagct ggccgaaaaa
      901 agcacctacc tggaaacggc ctatctgatt ttgcatggcg agctacccac gcgcgcgcaa
      961 ctggatgact ggacttacca catcacgcac cacaccttca tccacgaatc agtgaaaaaa
     1021 ttcctggatg gattccatta cgacgcccat ccgatgggca tgatgatctc cgccattgcc
     1081 gcgctctcca cgttttatcc cgaagcgaaa gacattttta acaaggaatc gcgaaagaaa
     1141 cagacgtatc gcctgatcgg gaagatgcca acgctggccg cgttttccta ccggcacagc
     1201 ctaggcatgc ctttcgccta tccggacaat gaactttctt accccggcaa ttttctgaac
     1261 atgttgttca agacttctga attgaagtac cgcccgaatc ccacacttga acgcgcgctg
     1321 gatgtgctgt ttattctgca tgcggaccat gagcaaaatt gctcagcaaa tgcgatgcgc
     1381 tgcgtgggca gttcgcatgt ggacccattt tccgccaccg ccgctgcaac cgctgctctg
     1441 tatggcccgt tgcatggcgg tgcgaacgaa gaagttctgc gcatgctcct cgaaattggc
     1501 tcgctcgcca atgtgcctgc gttcatcaag cgcgtgaagg ccggcgaaac caaactcatg
     1561 ggcttcggcc accgcgtata caagaactat gacccacgcg cccgaatcat caagcatgtt
     1621 gccgacggcg tcttcgaaat catgggacgc aatccgctgc tcaatattgc tctggaatgc
     1681 gagcgcattg ctctggagga cgactacttc gtttcgcgca agttatatcc gaacgtggac
     1741 ttctataccg gcctgattta tcaatcgatg ggtttcccag tcaccatgtt ccccgtgctg
     1801 tttgcgatcc cacgtaccag cggctggatt gcgcagtggg aagaaatgct gctcgacccc
     1861 gaacagaaga tcgctcgccc gcgccagatt tatacaggct acgatctgcg gccgtatgcg
     1921 tcgctcgacc aaagaaaata aatcggtttt actttttaat ttaaaaagcc tgtggcgagc
     1981 tatttctttt taggcggata tttgtcgatc gactttttga ccatgtcgtt gatcttgcct
     2041 tgaatcttgc tggcgtcact tttatcttcc gcggcaatcg agcgccagac gagcgagtgg
     2101 gtcgtcggat cgcgcaggca gatccatcac caaggttcct tcagcatagg gaactctcac
     2161 gtagcgcgtg cccattccgt accaaccggc gggataagct tccacttcac ttttgcgagc
     2221 tgcgccgaac gtgtagcgca cattgagatc cgccgggccg ctcgtgacca tcgtcaggcc
     2281 tttcgcggtt aaaccatttt gaatgtccac ctcgatctgt ttctttacca gttcgcctcc
     2341 caaggcagga tttctgctat tcagctggcc ttcacgaatg gcgaacgttt tgtacttgct
     2401 gaagtcggcg gtctgatcga actccatggt aattttttgc gctcgcgcgc tttgcgcgat
     2461 cagggctccc agaatcatga gcgcaatgcc acgtaatacg ggagcgacaa attggcgcat
     2521 ccgttgaact ggaagttgca aaaggaataa ttgagtcaca acttacctcc tcgaaaagtc
     2581 cgacgtagtg tatcaagact gccggaaata tcagctatgt tggtttttcg ttgtagagtt
     2641 tcagttatcc gctttccaaa cgtaggagag aacaatgcaa aagccctttt ttgctccaca
     2701 atcgtttccg atttataatg ggtttatgcg aaacacgggt attcattttg atgaatttgc
     2761 gacggcgcgc cgcgccatgg tcgaaacgca aattcgcaaa cgcggcgttt cttcggagcg
     2821 ggtcctcgaa gcaatggcga ccgttccacg ccacgaattt attcccgagc aatttcgcaa
     2881 tgacgcttat gccgacaagc ctttgccgat cggcgaaggc caaacgattt cgcaacccta
     2941 catggtcgcg gccatgaccg aggcgcttga gcttaagggc tcagagcgag tgctcgaaat
     3001 tggcacgggt tccggttacc aggcagcggt tctttcgact ttggtgagcc aggtgatcac
     3061 ggtggaaagc cagacatcgc tcgcgcttgc cgctcaggaa cggttgacaa aattaggcta
     3121 cacaaacgtg catgtgcata atggagacgg ctccgcaggc ttgcccgatg ccgcacccta
     3181 cgacgcaatt ctggtgacag ccggcgcgcc gcaaattcct caggtatttg caaaccagct
     3241 tcgtgaagga gggcgcatcg tcattcccgt aggcgatcgg gaaaatcagg acctggtgtg
     3301 cgcgcaaaag gaaaatggcg aattaaaatc gcacgtgctg ttcggttgca agtttgtgct
     3361 tttgttgggg cgatatggtt ggaatcgggt cgagagaaaa ttttcgtagc gttttccata
     3421 ggccagaact ttaaatttcg ttcgttgggc tcgtgcggct ctctggagtc aaatctcgtt
     3481 cctgagcgcg agcaaacgct tttcggagca cacgcttcac cgccacggtc acgtcaaatt
     3541 tcattaaatc ctcttcccgc gcccacgcaa attccagcgc atcactggcc gcggcaattg
     3601 ttccaccgcg aatttcacag agataatcga ggataacgaa gtggtattgc gggcgagccg
     3661 ggtcgcccgg cgtcccgtcg gtgttgggag gagccgggaa gattctctca aacacttcga
     3721 tgagttcgat cactcgcacg tcaagcccgg tctcttcggc aagctcgcgg accacgccgt
     3781 cttcgagcgt ttctcccacc tcgagcaatc cgccgggaat ggaccactct cccttgaggg
     3841 gcgcgctgcc gcggcgaatc agcaaggcgc gcccctcatg gaccaccacg cccccaacgc
     3901 ctaccatcgg ttgttcaggg tattctcttc tgggagtcat agtcgatttt ggctgcttcc
     3961 ctctcgagcc gcttcgcgtt atataatact tcagtagaag gtgtgtcttg tatctggcgg
     4021 gcttccactg agcctgtccc caatctccta ggaggtctta gccgtggtcc ggtgccctaa
     4081 atgctctacc gaaattgatg tggatgaaga cgaagtcgag gaaggcgaaa tcctggcctg
     4141 ccccgaatgc gaaaccgaaa tggaagtcgt gcaaatacat cccgtcaagg taaacgctat
     4201 ttccgaagac gatgacgaag aagaggacga agagtccgag gacggggacg atcttgagga
     4261 cgaagacgac gaggatgacg acgaagaaga agaggatatt tgatccgcgg tttcacctct
     4321 tcaggcatcc ctcttctcct atcaggtatc tttcttatgt gcgattgacg attgcatttg
     4381 tgggactcgc gttgctatcg ggcacaacat ctgcttttga gcagcccggc ggaccgcaaa
     4441 gttcgagcaa atcgcatgcc catgatttcg ttattttcac gaccgtattt acggagcagg
     4501 gattcgcgct ttctggtgca cgtgtgcgcg tcaggcggtc gaatgaaaag aaatttcgtt
     4561 gggaagccat gtcggatcgc cgcggcgagc ttgctttccg cgttccacag gattcggagt
     4621 acgaaatgac ggtcgaggcg cgcggttaca aaacgctaac acgcaaagtg gatggcactg
     4681 aaggtaatcg agcggacctg acgatacgga tggaacctct ttcggagccg cataccgatt
     4741 cagggaccgg aggaaaatca tgaacctgcg cggaacgctc gcaacgatcg ctttgattgg
     4801 attggccgcc acagtcatgt tcgctcaggg aaatccaaag ggaaaagaat cgcagctgcg
     4861 gaccgttcgg ggtacggtag tggacaacac agaaacccct tctgaaactg ccgtcgtata
     4921 tctcaaaaat acacgcacgc aagatgtgat tacgcatctt tccgacagcg atgggacgtt
     4981 tcgcttcagc gggctcgacc tcaacgtcga ttacgatatt catgctgaaa aagatggttg
     5041 gacttccgcg tcgcgatcga tttccaattt cgatacccgc aaagaattcg tcctcacctt
     5101 gaaattggat cgtaaaaagg gcggaaagta gtcgtagcat cttgcgttgc tgtagctcgc
     5161 ctcttcagag gcgagggttt agatcaaacg ctgggtactt ttcgtgggac aactaaagcc
     5221 ccgcctctga agaggcgggc tacagcagga tcatccgata cttgacccct acatcggacc
     5281 tcttgtttcc aagggtaagc atttaccttc tttccgatga ctcaggagcc cgatttgcag
     5341 taacatgctt tgcaccaaat cctggcaggc gttacagcgc atcagaatca aacatcggcg
     5401 tcgaaataag attttccccg cggaggattc ctatggcaat ccaatttttt gtaaacggca
     5461 agcctcaatc ggttgatgtc ccgccaaaca tgccgctgct gtgggtcttg cgcgacacgc
     5521 ttggaatgac aggaacaaaa ttcggatgcg gcatggccct gtgcggagca tgcaccgtgc
     5581 acattgaggg cgaggcgacg cgctcgtgca tcactcccat ctccagcgtt gccggaaaaa
     5641 aggtcacgac gatcgaaggc atttcgccgg atcgcagcca tcccgtgcag cgcgcgtgga
     5701 tcgaagtgga cgtgcctcaa tgcgggtact gccagtccgg ccaaattatg tccgccgtcg
     5761 ctctattggc gaaaaaccca aaaccgaatg acaccgaaat tgatgaagcg atgcggggaa
     5821 acatctgccg ctgtggaact tatcagagga ttcgcgaagc cgtgcaccac gccgcggatt
     5881 tgcacaaggc atctgcaact ccggcttcgt tcgatggagc aggcgctacg ctggaagcgc
     5941 gtaaccaaaa tgcggtagaa gccggcggtg ccgcttgagc tcgggaataa ccactccatt
     6001 aagccgccgc agctttctga aaacgagtgc gacaggcgca acggcctggt gatcggcttt
     6061 tatctgccgg acgtttcgcc gcgcaagcgg tggaaatttc agtggaccgg aaagcgcgct
     6121 cggtgaagat acaccgcgtt gtcgatttca gtggcatagg cttctgcctg tgccacgtaa
     6181 atacattctc cataagacga ttttcggtga atgaatttcg tcccgtgcag aaactaatta
     6241 gaaattcttc aacagcgcgg gcaaatcgcg aatcgtctcg atcaacgcat ccgcggacgc
     6301 agcgcgaagc cgttccggca caggcgatcc gcccagcacg ccgatggccc gaactcccgc
     6361 tcgatgcgcc atttcgatat cttctggagc gtctccgata taaacgctgg tatgaggacg
     6421 agcgcccaat ttttcgagcg ccgttcgcag cggcgccgga tgcggtttgc gtcgcggcgc
     6481 gtcctcgcag cacactttcg tcaggaacat ggctgataca ttgtgctggc gcaattgttt
     6541 ccgcacgcga gtgcggctcc cgctcgacac cagcgccagt ttaaaacgac gatccagaat
     6601 tcgcagtaca ttcatcgcgc cgggctgcaa cttcggcaat tgcttctgat aaaaacgtct
     6661 ccatagcctg tcggcttcgc catatttagc gcgaggaatt cccgcggcgc ggtacaccag
     6721 atgccaattc ggcgaataat ggcgcttcaa ttgggcaatg ctccagtcca tgccgagggc
     6781 gccgaacatg taggcgtatg cgttcgcgtc cgcctcgaag gaattgagca gcgttccgtc
     6841 ccaatcgaag aagatatgtt cgatcacaat tttatttttt gcgttcatcg cagcggaata
     6901 aaggtaccat gaaattgttg cgcgggaatg gcgttggaag gcgtgcagga tcgcgatatg
     6961 tcgcgatcct ccctgccatt ttggattcaa caatccgccg cgctcgaatg aagacagcta
     7021 gtggcgctgg aaacgcgagg gcgatggctc ttcgcgttga aatgtaatgt cgtcaaacgt
     7081 cgctgccagc atgtgccagc cgggacgcat agcgaaatcg gcgcgaacca cgtgcgcttc
     7141 cgtcagcatt ttcacggaac cacgtccgag cggccgctcc accagacaaa tttcgatctc
     7201 caccggggtg ccgggcgcaa tgcgatacgg gcacaggaaa agcactccgc tggaactgat
     7261 attcgttgtg cgcaacggtt ccattttctt ttcgcgctga ccagcgatgc gcttcacgcg
     7321 caaggctaaa ctgagatttg cgcgagagta ggcacggcgt tcctcaggcg gtcgcacggc
     7381 atgcgtggaa acgcgcgaga tacctttcgg ctttgctcgc gttatccacg acgggatgtc
     7441 ggctaccacc gtggccgaac tcgccgacaa ttcttcgata ccgcgatttc ccctgctggg
     7501 atcggattgg gacgtgagct tttgaattcg agaagtgcgc ataggtctcc cttaaattca
     7561 ggcgcctctg gcactgcagg ccgcaaatat cctccgtgcg ggataaagcg tcaatttgcg
     7621 ttcgttctta tgttgtctta ctgcatactc cctttgatat gaatcaatca taggcggcag
     7681 ctcatgccag gcgaccggta gaacagccgg tagaaaaaga tcagtttgac cgcaagatac
     7741 ggatagcgcc gcgcgaacga ctttcgttat tgtgttgaag aaggaatatg caagccatgg
     7801 cgacacgatc gtatccggga atgcaagcgc ttccgcccgt cgcgcgaaaa aaatatctcg
     7861 cggccgtcgc ttcgcatgac gcgcgaatgg atggcgcatt tgtgtacgca gttcgctcca
     7921 cgggaattta ttgccgtcca tcgtgccctt cgcggcgtcc gcatgaaaat caaattttgt
     7981 ttttcaataa gcccgaggaa gcagaacagg ccggctttcg agcctgcttg cgttgccatc
     8041 cgcgtgaaaa ccgcaacagc agcaaagtgc aactggttag aaaagtttgt aaacaaatcg
     8101 agagtcgccc tgatggctct gtttccctga agaaccttgc ggaacatacg ggcctcagcc
     8161 ccgcacactt gcagcggaca tttcgggagg ccatgggcat cactccgcgc caatacgcgg
     8221 aagcgcttcg cgtgacgcat ctcaaatcgg aattgagaaa ggggagcaac gtgacaaccg
     8281 cacttcatga agtatctttt ggttcaacga gccgccttta tgaaaaatct aatgcgcaac
     8341 taggcatgac gcctgccact tatcgcaaag gaggacaagg aatgaatatt tcttacacga
     8401 tagcggatag ttcgatggga cgagttctcg tcgccggcac ggaacgcgga atttccgcgg
     8461 tgtaccttgg ggataaagac tccgacctcg ccgcttcctt gcacgaggaa tatccgcaag
     8521 ctgagattca aaccatcccg gaaggaaaat cgaagtgggt acgcgcaatc gttgcgcacc
     8581 tggacagatc gaattcgccc ctggacttgc ccacggatgt tgcaggcact gcgtttcagc
     8641 gccgagtttg gcaagcgctt cgcgaaattc cactcggcgc tacgcgcact tattcggatg
     8701 tagctcgcgc cattggccag ccaactgcca ttcgggctgt aggccatgcc tgcgcgacaa
     8761 atcctgcatc gattgtggtg ccctgccaca gagtgattcg cacggatgga actttgggcg
     8821 gatatcgctg gggccttcat cgcaaacaat ccctcctgga aaaagaacgt cgcgcagcga
     8881 aggccaagta gcgcggggct tcagtgtctg tgtaagaact gcaaaattga agcttcagcg
     8941 gctaaagccg aacttatttt gcagcactta tgtcgtggct aaagccacga cccacaaaga
     9001 gtttgaagtt ctcacacaga ctttgaagcc tatgcctcgc aattgcctgg caaatctgcg
     9061 taggtaaata aacctacgct tgcggttgca cgacggtagt tccgtgaggc cagtgaggtt
     9121 taaattcgct atccgcaatg cgtgcattgc gaatcatatt tttatagtga atgatgaagt
     9181 agtcgcccga tcctgtctca aaaaattgct gctgcacggg cagccaggtg gattcatcca
     9241 gccatagctg aattttggaa agctgcttgc gcacttcatc cgtcttgggc aaaagctcca
     9301 gccggacaac tttgtgacca tcaagtgtct cttcgccctg cacggcaacc gtgtaacttt
     9361 ctttcaaaga actcccggac gtaccgaatc cgagcagcaa gaattgatcg acgagggatt
     9421 ttttctttcc aatgttgtac tcctcgacgc ggtgaatctt cggattgtaa atatcgaaaa
     9481 tatcgccgtt gcggagaatg gttcgcgcgt caggttgcgt tatttcgatc agcatcttgt
     9541 catcgcgacg gacggaaata cggccggact ccgtagactt atcgttcacg actacagtca
     9601 ctttggtgcg ctccatgtca gccgtgaggc tacggaaatc cgcggcttgc gcgtccatct
     9661 gtttcataac attgtcgagc gtccaactgg actgcggagt tgcggaaagt aacggcgtga
     9721 aaaaaaatac cgcagcgagc gccgctgcgc agaacgatac acacaatcta taaattgcgg
     9781 ttggaagttt cattaaaccg gctgaccccc tgatccattt gcatcggcct atcctggctt
     9841 ttttgcggaa atgcaggtcc cgaccgtgcg gcaatcgtac catggacgtt aagatgccaa
     9901 ctcttcgtct ggggttacga tttctgcagt tcacaacagg atgacgcaat gggttcttcc
     9961 cccaatccgc cgaaaggttc tgtatgatgg tcggcctatg ttccatcgca aacggtttcg
    10021 attgcgtctt cgctcgcgcg cgctgatcct tggcgagcgc acgctcatta tgggcgtttt
    10081 gaacatcacg ccggacagct tttctgatgg cggagcattt ctcgattccg aagctgcaat
    10141 cgcgcacgga ttgcagctgg aaaaagatgg cgccgacatt ctggatattg gcggcgaatc
    10201 aacccggccc ggtgccgctc ccataaaacc tgaggaagaa attcgacgca ttttgcaggt
    10261 catccagata ttgagcggca agctgggcat tcccatttcg gtggatacgc gaagagcgga
    10321 agttgcaaaa caggcgctcg acgccggggc cgaaatcgta aatgacgttt caggattgcg
    10381 atcagatgcg caactcgcca atgtggcgcg gcttgcacgt gcacccttga ttctgatgca
    10441 catgcgcgga acgccgcaga cgatgcagcg cggccccttc tcgcgcaaca ttattcgcga
    10501 cgtaagatcg gaattacgcg actcaatggc tcgcgcaaag aaagccggca tttccaaaac
    10561 gcggattctt ctcgatcccg gtatcggttt cgggaaaacg ttcgagcaaa attttgagct
    10621 tcttgcccga ctgcctgagt tcgcgcgcat cggctgcccc attgtggttg gcacatcgcg
    10681 aaaatcattt ctcggcaaag ctctcgcgga ctcgggcggc gcgagtgttc ctgccgaaga
    10741 gcgtttattg ggtactgccg ccactatgac ggcttcgatt ctcggcgggg ctcatattgt
    10801 tcgcgttcac gatgttgcgg aaatggttcg cgtcgcgcgc gttgcggatc acatcgtgaa
    10861 cgctacgcgc agttgaatgc gctataaagg acgcgaaact aaggcaatgc aggtgcacaa
    10921 ttcatccgtt gggatataat tccacttggt ttcgtgcgct ccaaaatgcg cgcaaatagc
    10981 tgtatcgcga tgaaattcaa caaaatgctt ccggcgcttc ttgcaattct cctttccccg
    11041 tgcctacccg cgggtgccca attgacgcca gcggagcctg atccgctggc gcgtatccgc
    11101 gatgcggctg cggcgaatgt gcaagcctgt tccgcgaccg gagaaacttt atgcgagcag
    11161 gtcgcgccga aaatcatcgc caacgcacag ggcgattctc cgctggctga aaatcttcgc
    11221 ctccttagaa aaaatcttgc gacgaggaat aaattcgttc aagaggggcg tgcggttgag
    11281 tgggccatgg cagcctttcg cgaaaccgga ctcgatccgc acattgagaa atatgcgaat
    11341 cccaatggca gcgcgggcga acaacaaaat gttgttgcag aatatcgcgg gcgggacaat
    11401 tcaaatgaat gggtcctggt tggcgctcac ctcgcacctc gatcaaatgg atctagtgca
    11461 gtcgagaaca tctgcgatgc tgcctccgtg atcgaagccg cgcgcgatat tgcactaaca
    11521 gccatgcatc cgaaacggtc tattcgcttc gttcttttca ctggcgatga gcgcgacatg
    11581 ctcggttcct gggcctatgt gcgctcccac cgcgcggaac tcgatcacgc tcgtgcggcc
    11641 attatttttc attccaattg ctatcgagtg tccggatatt cgctcaatgg cggacttgat
    11701 atcgaagccg gcctccgcga tgcgatgaaa cctatcgaat ccctgggagc tactcatttt
    11761 gtcactggcg ctaagacagg aacggacgat ttcgattttc tcctcgaagg cgtacccacg
    11821 ctgtcggcct atccgattct gatgccagtt tcagtcgatt ccccaatgct tctccccaac
    11881 tccggaaaaa ctgagctggc tgacctcaag cacaatgtcg ctgtggttgc cgtgactgcg
    11941 tttgggattg ctgagcgtac agagcccatc ggtccgcgac aatcccacgc tgaaatcgaa
    12001 ttgctgctcg aaacgtttgg tcttgaattc caaatgaaaa ccgcgggaac ctggaagcaa
    12061 tgggaatcag gcgaacgcgg gcgtttaccc taaacgaaat gcccggtcta tcccgccctc
    12121 ggaaaaagcc gggttcgcga tataatatgg ccgtgccaat tcccctccgc gctctatccc
    12181 tggcggccga tccccccact ccgggtgtga ttctcgcaat tgattacggc aaaaaaaggc
    12241 tggggctggc gctttcggac gagtttggag tcacttcgcg tcccttcgcg acatggactc
    12301 gcattaaccg ccgccgcgat ctctcccgcc tccgcgacct cgtacggcag gaaaaaattc
    12361 gccgcatcgt cgtggggctt cctctgcgtt tggatggcac gccaagcgaa atgtccgaag
    12421 aggcaaaaag ttttgccctg cgcgttgaaa aagccatcgg ccttcccgtg gaaatggttg
    12481 acgaacgcct aagttcatgg gaagcgcggg aaacagtttc gcaaatgaat tccaacaagc
    12541 gacctcggag atcttcagac cgcggtgggt catcgaagaa aactccgatc gacgatatcg
    12601 ccgcggcgat tatcctgcga gattacctgg atcgcacgca aacgcggccc ggatcgcgag
    12661 gctgatatgc gccgggcact cctctatcta ttgattttgg cgatcctcgc tgtgggatac
    12721 atttgtgtag cgctctacgt tccgtatcaa ggtttcggca gtgacggcgt atatgtagat
    12781 attccgcacg gcgcttcaca gcgaactatt tcccgcctgc ttgctgaaaa cggagtggtg
    12841 cgcaaccggt tcgcttttga gcttctagcc cgcctgcgaa aacgacgcac gatgcaagcg
    12901 ggtgagtatt tcttcaatcg cccggtcacg gctttcgacg tgtatgacac gatcgtcaac
    12961 ggccgcgtct tcgtaaagga attcgttatc cccgaaggct ttacgatgtt tgacattgcg
    13021 gacctcgccg cgcaggaagg ctttcttacc cgcgatgaat ttttggcagc tgcgcgcgac
    13081 ccttcctcga tcagcgacct ggctccagat gcgcccagcc tcgaaggatt tcttttccct
    13141 gcaacctacg aattcccgcg ccacatgatg ggaaaagaga tgacatcggc catgacgcgg
    13201 aaatttcgac aggagtggct cgcattgtcc gatgcggcgg caacgactca tccaaatgtt
    13261 caaagcgtcg tgacactcgc ctcgcttgtc gagcgcgaaa ctccgcgtcc agaggagcgc
    13321 ccgcacgttg cgggagtttt tgaaaatcgg ctgcgcattg gccagccgct gcaatgcgat
    13381 cccacagttg cctacgccat gactcttgcg ggcgccttct ccggaaaact gagcggcggg
    13441 gatttgcgtt tttcttccgc ttacaatact tacgaaaaca ggggcctgcc gcccggcccc
    13501 atcgccaatc cgggcgaagc agcgctgcgt gccgcaatgg acccgcctcc gacggacgat
    13561 ctctactttg tcgcaaacac ggaaggcggg cactttttca gcaagaccct tcaggagcat
    13621 aatcataatg tcgcgcggta ccggcatctg cttgaagagt cggagaaacc ctcggccggt
    13681 agcgcacctc caggcccagc aaaaaataaa tccaaacctg cggggtagcc acaatggcgc
    13741 gtggggcgag cccgaccaag aaaaaagtaa ttctggaaat cgcgggcgaa cttgccgtgc
    13801 cgcgattcac tccggcagag gtggagcaaa ttcgccggca gcttgtggcg cgcctcgggg
    13861 cagctggcag aacttccgct gaatacatcg caggtgtgct ggaaaccgcg ggcatgcgca
    13921 ttgtttggtc caccaaggcg gatacagagg gccactacga ggaagaattt cacaacttgc
    13981 tgcacttcgc gaatctggcg gacgcggaac tgtgcatcat gcgcctcgat gagctgtacc
    14041 ggaaatttca ccaggaaaaa gaacatgcgg caacggagcg cgtgttagag gtggcgcggc
    14101 tcggccggcg ccgcgcggaa atgatcgcgc ggaaccacaa ggtagaaccg gaaaagcggg
    14161 cggaaaagga agagatcatg cagtggttca aagtttggct ggaaacaccg acggcttttt
    14221 tcgattggct ggaagcgcgc aaagccacgc cggatttcaa gaagcgtttt gccaagagcg
    14281 attccgcgga cgcgtagaca aatgatcaag gatccgattt atgttttgga agtggagccg
    14341 ctcgatctcg gagcagatct taaggctgtg ggactcgacc ttgtggagat cgaaagccgc
    14401 gaaccggtgc gcggcgtgga cgcggctaag ctgtgggccg cagcgctcac tgcattgccg
    14461 ggagctgagc catggggatt cgattttttc gcacacctcg atcgcgtgcg cgagtattgc
    14521 cgcatgcatg gcgtggcatt tgaggaaaaa acttcaggca atagcatcgt aattccctcg
    14581 ccaatgggtg atgcgctggc cgctcttgtt gaacgatttg ccggggaaac atttggcatt
    14641 cgcgcggggg ccttatttgc cgctggtgat tccgcggtcg aaggtgagct tgcgaagcac
    14701 ggcgtggacg gctatcacag tgcatttaga aattatttat tttgcgcggt ctgcgatttt
    14761 gaaaatgggt ttttgacgct gctcactgaa aaattgtgga ccagcgaagt gctgcgaagg
    14821 attaaacccg caaccgcggg catgccggtg gaagtcgccc gcccgcagta agaataattt
    14881 aggtttttga tattgaaagc gtgcaggacg aaagacgatt ttcgtggcat aggcttcagc
    14941 ctgtgggggt tggtccttgc gaggacg
//
LOCUS       DASZMB010000053         5183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4445127, whole genome shotgun sequence.
ACCESSION   DASZMB010000053 DASZMB010000000
VERSION     DASZMB010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4445127"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /gene="kdsA"
                     /locus_tag="VG272_02300"
     CDS             complement(<1..519)
                     /gene="kdsA"
                     /locus_tag="VG272_02300"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HWF12539.1"
                     /translation="MQVAREISIGSVRIGGNQPLFLIAGPCVIENEEHAMMMAEQIKG
                     IAAHANVPFIFKASYDKANRSSDKSFRGPGLADGLRILQKIKERFGLPILTDIHEASQ
                     AISVADVADVLQIPAFLSRQTDLLVAAGKTGRVINLKKGQFLSPWEMVNAVEKVALTG
                     NDKIFLTERGASF"
     gene            complement(690..2360)
                     /locus_tag="VG272_02305"
     CDS             complement(690..2360)
                     /locus_tag="VG272_02305"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HWF12540.1"
                     /translation="MGPKYIFVTGGVVSSLGKGVAAASIGCLLESRGLRVTLQKCDPY
                     LNVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFTHAKLTRENNWTTGRIYESILTR
                     ERRGDYLGKTVQVIPHVTDEIKSAFRKIAVDVDVVIVEIGGTVGDIESLPFLEAVRQM
                     RLELGPENFVHVHVTLVPYIPTAGELKTKPTQHSVKELLSIGIQPDILLCRSDRTVPQ
                     ELKTKIALFCNVPEKRVISMPDMDTIYAVPVALAEEGLDDQILKLLHLEARERDMSAW
                     LELLERIRKPKGEVRIAVVGKYVQLEDAYKSLRESLIHGGLAHKDRAIIEWVEAEELD
                     SRETAAKHLEHADGILIPGGFGKRGIQGMIYAIEYARSHKVPFFGICLGMQCATIEYA
                     RDVVGLSGADSTEFDPQTPHRVIYKLRELLGVDEMGGTMRLGAWPCKIEKDSTAYKAY
                     GSTEISERHRHRYEFNCEYEKVLTDAGFRITGRSPDGKYVEIVEAPNHPWFLACQFHP
                     EFKSTPLVPHPLFYAFMGAAHTHQHRANGAAGGKPSAISMTAKPSQAD"
     gene            complement(3620..4882)
                     /locus_tag="VG272_02310"
     CDS             complement(3620..4882)
                     /locus_tag="VG272_02310"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWF12541.1"
                     /translation="MFKKAAAMSLAVAMMAAPMFANEGDVPKGVPRLDHVFVIMMENH
                     AYGQIVGNPSAPFTNKLMQSANAANNYFAVGHPSLTNYLEVVGGSNFGVETDNSPDWH
                     NTNCVTNLASGTVATDNPASPNVCPIWGTGTDGATPVFDFTNETAPPSITEVTNVDGV
                     KSFPAAKNISGKTIADQLDEHGMSWKSYQESLPAAGADGVNNSDGYFSNLLSITSVLP
                     AETQTLINLYAVKHNPFAYFQNIQQGNDADSSLKNMVGFEGKRGLFEDLESGKVPNYS
                     FIAPNQCNDQHGRGNAGPVCDFDPSTLGTQVGLNPALIYQGDLTLRNLVKAIHASPAW
                     SEGHNAIVILWDENDYSATPNVNQVLLIVDTNYGAKGVQSSQFYTHFSLLKTIEGGFG
                     LPCLNHACDAETKVMSDLFGHDDHDGKW"
BASE COUNT         1121 a         1462 c         1353 g         1247 t
ORIGIN      
        1 gaacgacgcg ccgcgctccg ttagaaaaat tttgtcgttg cccgtcagcg ccactttctc
       61 cacggcgttg accatttccc agggcgaaag aaattgccct tttttcaaat tgatgacgcg
      121 ccccgttttt cccgcggcca ccagcaaatc cgtttggcgc gataaaaacg ccggaatctg
      181 cagtacgtcc gcaacatccg caaccgaaat tgcctgcgat gcctcgtgaa tgtccgtcag
      241 tatcggcaaa ccaaatctct ctttaatttt ttgcaggatg cgcagtccat ccgcgagccc
      301 cggcccgcga aacgatttgt cggatgaccg gttggccttg tcatacgacg ccttgaaaat
      361 aaacggcaca ttcgcgtgcg ccgcaattcc cttgatctgc tcagccatca tcatggcgtg
      421 ctcttcattc tcaatcacgc acggccccgc gatcagaaac agcggctggt tgccgccgat
      481 gcgcacactt ccaatcgaaa tttccctggc aacctgcata ttgaaaaatc cctcggaaat
      541 aaaagcgatt catagcctat gcgggaccag caagttttgc aacgccttga gtaggacagg
      601 cttcagcctg tctgcatttc ttctcggcga aaacaatcta aacctgacag gctgaagccc
      661 gtcctactgg aggcccgcat tacaaattat caatcggctt gcgaaggttt tgcggtcatg
      721 gaaatggccg agggtttccc gccggcggcc ccatttgcgc gatgctggtg cgtgtgcgcc
      781 gcgcccataa acgcataaaa gagcggatgc ggcacgagcg gtgtggattt gaattcagga
      841 tggaactggc acgcgaggaa ccagggatgg ttcggcgcct cgacaatttc cacatacttg
      901 ccgtcgggag aacgtcccgt gatgcggaat cccgcgtcgg ttaaaacttt ttcatactcg
      961 cagttaaact catagcggtg gcggtggcgc tcgctgattt ccgtgctgcc gtaggccttg
     1021 tacgccgtgg aatctttttc gatcttgcag ggccacgcgc caaggcgcat cgtgccgccc
     1081 atttcgtcca cgcctagtag ttcgcgcaat ttgtaaatca cgcgatgcgg cgtctgagga
     1141 tcgaactccg tgctgtccgc tcccgacagc ccaacaacat cgcgcgcgta ttcgatcgta
     1201 gcgcactgca tgccaaggca aatgccaaag aacggaacct tgtgcgagcg cgcatattca
     1261 atcgcataaa tcatgccctg aatcccgcgc ttgccgaagc cgccgggaat caggatgccg
     1321 tccgcatgct ctaaatgctt cgccgcagtt tcgcgcgaat cgagctcctc ggcttccacc
     1381 cactcaatga tggcgcgatc cttgtgcgcg agcccgccgt gaatcagcga ttcgcgcaaa
     1441 cttttgtagg catcttcaag ctgcacgtat ttccctacta cagcgatgcg cacttcgccc
     1501 ttaggcttgc gtatgcgctc cagcaactcg agccatgcgc tcatgtcgcg ttcccgcgcc
     1561 tcaaggtgca gcagttttaa aatttggtcg tccaggcctt cttccgcgag cgccacgggg
     1621 acggcgtaaa tcgtgtccat atccggcatg gaaatcacgc gcttctcagg cacgttgcag
     1681 aagagcgcga ttttcgtttt tagttcttgc ggcaccgtgc ggtcgctgcg gcacaacaaa
     1741 atgtccggct gaattccgat ggaaagaagt tccttcacgg aatgctgcgt cggcttggtt
     1801 ttcaattccc cggctgtagg aatgtacgga accagcgtga cgtgcacgtg cacaaaattt
     1861 tcaggaccca gttcgaggcg catctggcgc acggcttcca gaaacggcag agattcgatg
     1921 tctcccaccg tgccgccaat ttccacgatc accacatcca cgtccaccgc gattttgcgg
     1981 aacgcggatt tgatttcatc cgtgacgtgc ggaatcacct ggaccgtttt gccgagatag
     2041 tcgccgcgcc gctcgcgcgt caggatcgac tcgtaaatcc tgccggtggt ccagttattt
     2101 tcgcgcgtga gtttggcgtg cgtgaagcgt tcgtagtggc cgaggtccag atcggtttcc
     2161 gcgccgtcgt ccgtcacata cacttcgccg tgctgatacg ggctcatcgt gcccggatcg
     2221 acgttaagat aaggatcgca cttttggagg gtaactcgga gaccacgact ctcaagcagg
     2281 cagccaatcg acgccgctgc aacacccttg cccagggagg atacaacacc accggtaaca
     2341 aaaatatact tcggccccat ccgctcagtt ccagtcgcct caaattagaa gaatacaaga
     2401 cgcagaactg ggtaaaacct ccacaaagat gttggcgtac aacgcattct agggggaaag
     2461 atgcgccact gtcaatgcgg acgagatctc aatcgaaatc caacccaaga cacctcgaaa
     2521 cctgtccata tttccgtatg tatagtccac aatttgggct cttgactttt gcaagggtgg
     2581 tgtactatta aggtaacatg tcaacgaggt ctgcgctccc tgcctctatc ctcattccgg
     2641 cttttgcgaa agggcttccc ggcgcgtgcg caacgacgtt cttggcaacg atgcttctgg
     2701 gccaattcat tgctgactcg ctaatgggtc caagccaaga tcacggctgg aatgtattac
     2761 acgcattatg ctgggcggtc gcaagtattg ttgctggcag caccgcatgt tccaggctct
     2821 tcgatggaac ctacagattc gcgtctgaaa caacacgtac aagaatacgg gtgatggcgg
     2881 gttcggtgct ggctttggcg ttcgcaaaca tatggattga tatcctttct tactatttga
     2941 agaaatctgg gatacctcac tggagagcgg cctggatcgt tctagccatt ccagtattct
     3001 gcggcgaata ttattgctac cgcaaaatca aacaactcgt ctctgatttc gcgcaaccaa
     3061 ccgcatccgc atcgcagcac gtctagccgt cgaccacctc tgaagttgcg atatttcatc
     3121 aatttcaaac cactagtgta ctatcaagct aacatgccag ccgcgctgac tcatcctgcc
     3181 gcacctctca cgccatcaat tccgcaagaa cttcctaatc attgcctgtg ggctttgtta
     3241 tcagctcaga tggtgggcga acatgcagtg aggcgcggag cagcctactt gaagtggcct
     3301 tggatgttcc tggtcttgct tgtattgttc ggtgatgtgc tgtgttatct caaaatcaag
     3361 cgccttgtct ctgattttgc gcatccaacc gcgccgttcc cgcgacccta ctagcaatcc
     3421 ggcatatcgc tgcacaagct cacccgtaat gtagcgccgt cgtattgccg tgagtggcat
     3481 cttctattac ctgcgtgaaa ccaaggccct tgaatcctct cccaggtagc tcgagtacta
     3541 cttaatttcg ccaagctcga ttgcgcgaaa ctcaagtaaa attccgcgca atcggcccgg
     3601 cagccgtttc taaagcaaat taccacttcc cgtcgtgatc gtcgtgcccg aacaaatcgg
     3661 acatgacctt ggtttcagca tcgcaagcgt ggttcaagca aggaagcccg aatccgcctt
     3721 cgatggtttt aagcagcgag aagtgcgtgt agaactggga actctgcacg cccttcgcac
     3781 cgtaattggt gtccacgata agcagtactt ggttcacgtt cggcgtggcg ctgtaatcgt
     3841 tttcgtccca caaaatcacg atcgcgttgt ggccttcgct ccatgccggg gaagcgtgaa
     3901 tagccttcac cagattgcga agcgtcagat cgccctgata gatcaacgcg ggattcaatc
     3961 caacttgtgt gcccagcgtc gacggatcga aatcgcacac agggccggca ttgccgcgcc
     4021 catgctgatc gttgcactgg ttcggagcga tgaacgagta attgggaacc tttcccgact
     4081 ccaggtcttc aaagagtccg cgctttcctt caaagccaac catatttttc aggctgctgt
     4141 cggcgtcgtt tccctgctga atattctgga agtaggcaaa gggattgtgc ttcacagcgt
     4201 agaggttaat cagcgtttgc gtctcggctg gcagcacgct ggtgatcgaa agtaaattgg
     4261 aaaagtaccc gtcggaattg ttgacgccat cggctccggc cgcgggcagg ctttcctgat
     4321 agctcttcca gctcatgccg tgttcgtcga gctgatcggc aatcgtcttg ccggaaatat
     4381 ttttcgccgc cggaaaagat ttaacgccat cgacgttcgt aacttccgta atggagggcg
     4441 gagcggtttc gtttgtaaag tcaaagacgg gcgttgcgcc gtcggttccg gttccccaaa
     4501 ttgggcagac gttggggctg gcgggattgt ccgtggccac ggttcctgac gcgaggttcg
     4561 tgacgcaatt ggtgttgtgc cagtcgggcg agttatctgt ttcaactccg aagtttgatc
     4621 cgccaacgac ctccaggtaa ttcgtgaggc tcggatgtcc gacagcaaaa taattatttg
     4681 ctgcgttggc cgattgcatc agcttgttgg tgaatggcgc gctcggattg ccgacgatct
     4741 ggccgtacgc atgattctcc atcatgatta caaacacgtg gtccaagcgg ggcacgcctt
     4801 tcggcacatc gccctcattg gcaaacatgg gagcggccat catggccact gcgaggctca
     4861 ttgctgcggc tttcttaaac ataaggtgaa tcacctttct tcttcgggaa gctgggattg
     4921 ccgccgctct ggggacgcag agggctggcg cgaccagcgg tactttgaga gcggcgcatt
     4981 gcgacgcggt tacaggtttg cgaatattcg gtgaacgaac tccgcgaagg cccctgcatc
     5041 ccagatttta aattttgttg gccagcggtt tgctggtgtt catccgcgct tctatagtct
     5101 agaatcgttc cttcgttttg ccgcgagggg tttaacacgt tgcggaaaac tcaagctctg
     5161 tcattccgag cgaagcgagg aat
//
LOCUS       DASZMB010000054         5258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2258724, whole genome shotgun sequence.
ACCESSION   DASZMB010000054 DASZMB010000000
VERSION     DASZMB010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2258724"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..387)
                     /locus_tag="VG272_02315"
     CDS             complement(112..387)
                     /locus_tag="VG272_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12542.1"
                     /translation="MLFENRPGRRRLFRKGDAYDPAREGSKMTPNPDEIPIEYVDLLA
                     WYRDWSSAATIASSATDPLLALAGSGKELWADEHADEYIRRLREGWE"
     gene            755..1180
                     /locus_tag="VG272_02320"
     CDS             755..1180
                     /locus_tag="VG272_02320"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HWF12543.1"
                     /translation="MRTKVLFLCIGNACRSQMAEAIAKHMASDVIEPSSAGLVPFGEI
                     PSTTLCVLEELGISAEGQRSKPLRPADLSGADIVVNMTGRPGKLIFTEPVTAIEDWDV
                     GDPYGFNLSVYRGIRDQIETRMEDLARRLRQKDETRHSV"
     gene            1223..1894
                     /locus_tag="VG272_02325"
     CDS             1223..1894
                     /locus_tag="VG272_02325"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HWF12544.1"
                     /translation="MKREKTSVSREDYLKAIWEMVQEGQAPISARLAEELGVTPPAVT
                     AALKRLTRDGMLVLRRDGHIALTRKGSSLADRLAMRHQLAEKLLTEVIGLDWTRAHDE
                     AELLEHGISPEVEKLLLERFGAEGFCPHGVPLQGGLAKLRRKYGAVPLSDVETGRIVE
                     ILCVYEKDPEFLRFLSGLTLHPGARARIRNREYDETMTLAVGNRTIHLGKPATSRIWV
                     RALTQ"
     gene            complement(2178..3683)
                     /locus_tag="VG272_02330"
     CDS             complement(2178..3683)
                     /locus_tag="VG272_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWF12545.1"
                     /translation="MATTVPMPSLDRNVSQFIAKTHKILINGKWVDAASGKTFPTYNP
                     ATGEVLSKVAEGDKEDVDRAVKAARAAFETGPWSKITPSQRGRMIWKLGDLIEQHLEE
                     FAQLESLDNGKPLTVARVADVPLAADLFRYMAGWATKIEGNTIPISSGGGKTQFLAYT
                     VREPVGVVAQIIPWNFPLLMAAWKLGPALATGCTVILKPAEQTPLSALLLGELVAEAG
                     IPDGVVNIVPGYGETAGAALAAHPDVDKVAFTGSTEVGKLILQAAAGNLKKVSLELGG
                     KSPNIILDDADVESAIPGAASAIFFNHGQCCCAGSRLFVESDIFDKVVDGVAQHASKI
                     KVGPGMDPSSDMGPLVSEEQLNRVCGYLESGLSEGAKAVTGGSREGDKGYFVKPTVLV
                     NTNDKMKVVREEIFGPVVTAIPFRNVEEIAAQANNSNYGLAAGIWTRDIKKANRLASL
                     LRAGTVWINCYNVFDAALPFGGYKESGWGREMGHEVLEQYTEVKAVCTPVS"
     gene            3959..4492
                     /locus_tag="VG272_02335"
     CDS             3959..4492
                     /locus_tag="VG272_02335"
                     /inference="COORDINATES: protein motif:HMM:NF009807.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal-dependent hydrolase"
                     /protein_id="HWF12546.1"
                     /translation="MAGEDLRYPIGKYEAKSLLTSPERSLAIAQIEETPKRLCEAVGR
                     LSVGQLDTPYRPDGWTVRQVAHHLPDSHMNAYVRLKLALTENEPIVKPYEEALWADLA
                     DSKETPVEVSLSLLEFLHLRWDSLLRAMQPGDFAHRLRHPVNGVMTVDNLVHHYAWHG
                     RHHVAQITSLREREGWN"
     gene            4496..4729
                     /locus_tag="VG272_02340"
     CDS             4496..4729
                     /locus_tag="VG272_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12547.1"
                     /translation="MIFAALRYYWITAKGYRLRPWASPYLRWRLETFFGGDMHALGAA
                     EFFRLGWRERKRLGRFLAWTEERRRAQRAEGLR"
BASE COUNT         1165 a         1383 c         1479 g         1231 t
ORIGIN      
        1 gttggtcgcc tcgcgttagc attgccttcc gaagattctt cacgagttca aagtgcggac
       61 catgactttc cagcagatag atgaataaat tggaatccca aaatacgcga ctcactccca
      121 tccctcacga aggcgccgta tatattcatc ggcgtgctcg tcggcccaaa gctccttgcc
      181 cgacccggct agcgccagga gcgggtccgt cgcggaagac gctatcgtag cggcactaga
      241 ccaatcacga taccaagcca gcaaatcgac atattcaatg ggtatttcat ccgggttggg
      301 agtcatttta gaaccttcgc gggcgggatc gtaggcgtca cctttgcgaa acagccttcg
      361 cctccccggg cggttctcga aaagcattcg gcaccgggca gggtttgggg gccgattggc
      421 gacacaatgt tggactacat gaacatagac acctggccga attgagcctg caatgcgctg
      481 cttttctgcc cggtcaacta tttcatcgat ggaaaaatcg agctgttctg gattttcacg
      541 atgcaatagt gccgtagcaa tccaaacttc atctgccact ctgattcggg tgcgtgatcg
      601 tgtgggcatg tgtcccctct taaggtaaga atacaatgta tttatgtatt gtcaataatt
      661 gtttgtctta atcgctttca gtcggcttga gccttggtgg aattcgtgtc attctgatcg
      721 cccggaagcc agcccggaaa gatgaaaaat ggtcgtgcgc acgaaagtat tatttctttg
      781 cattggcaac gcctgccgca gccagatggc ggaagcgatt gcaaaacaca tggcatccga
      841 cgtgatcgag ccttcgagcg cggggctcgt gcccttcgga gaaattccgt cgaccacttt
      901 atgcgtgttg gaagagctcg gcatttcggc tgaaggccaa agatccaagc ctctgcgccc
      961 ggctgactta tctggcgcgg acattgtagt gaacatgaca gggcgtcccg gtaagttgat
     1021 ctttacagag ccagtgacgg cgatcgagga ttgggacgtt ggcgatccgt atggatttaa
     1081 cctgtccgtt tatcgcggga ttcgcgatca aattgagact cgcatggaag acttggcccg
     1141 caggcttcgc cagaaagatg aaacgcggca cagtgtttag gcgggccatg cgcgggttcg
     1201 gatcgattta aaaaggagaa tcatgaagcg cgaaaaaacg agcgtgtcgc gcgaagatta
     1261 cctgaaggcc atctgggaga tggtccagga aggtcaggcg ccgatcagcg cgcgcctggc
     1321 tgaagaattg ggagtgacgc cgccggcagt gaccgcggca cttaaacgtt tgacgcgcga
     1381 tgggatgctt gtcctgcggc gcgatggcca catcgcactc acgcgaaagg gcagcagcct
     1441 tgccgaccgg cttgccatgc gccaccagct cgcggaaaag ttgctcaccg aggtgatcgg
     1501 tctcgactgg acgcgcgctc atgatgaagc cgagctcctc gaacacggaa tttcacccga
     1561 agtggaaaaa ttgttgctgg agcggtttgg cgcggaaggt ttttgtccgc atggcgtgcc
     1621 gttgcagggc ggcctcgcaa aattgcggcg caaatatggc gctgttccgc tttccgatgt
     1681 ggaaaccggc cgcatcgtgg aaattctgtg cgtgtacgaa aaagatccgg aattcctgag
     1741 atttctttcc ggcctcacgc ttcatcccgg cgctcgcgcc cgcattcgta accgcgaata
     1801 cgatgaaacc atgacgctcg cagtcggcaa ccgcacgatc catctaggaa aacctgcgac
     1861 gagccgcatc tgggtgcgcg cgttgacaca ataatccagt cgtctattcc acaaaaagtt
     1921 ttttagattt acatccgttt atgttgatct tgtcatgctc gacgtgattt caggagtccc
     1981 gaagggacga tacaccgtag cccaccgtta aaacggtggg ctacgatctt tcgtccctga
     2041 cgggactggc tatgcgaggc gttctgatag cagtctgcac acattcgcga aatgaaaaag
     2101 ggccgagaaa actcttcggc ccttttcatt tttcatcgtt atttctcttt tgcaacgact
     2161 tcgctaattg cgtcttacta cgaaaccggg gtgcacacgg ctttgacctc ggtgtattgc
     2221 tcgaggactt cgtggcccat ttcgcggccc caaccggact ctttgtagcc gccgaagggg
     2281 agcgctgcgt caaacacgtt gtagcaattg atccacacgg ttccggcgcg gagtaggctg
     2341 gctaacctgt tcgctttctt gatgtcgcgg gtccagattc cggccgcgag accgtagttt
     2401 gagttgttgg cttgtgcggc gatttcctcg acgttgcgga aaggaattgc cgtaacgaca
     2461 ggtccgaaaa tttcttcgcg caccactttc attttgtcat tggtgttcac gagcacggtg
     2521 ggcttgacga aatagccctt gtctccctcg cgcgaccccc cggtgacggc ttttgcgcct
     2581 tctgaaagac ccgattcgag gtagccgcaa acacgattca gttgctcctc ggaaacgagt
     2641 gggcccatgt cgctcgacgg atccattccg gggccaactt taatcttgct ggcatgttgc
     2701 gcgacgccgt cgacgacttt gtcgaaaata tcgctttcga cgaacaggcg tgagcctgcg
     2761 cagcagcatt gtccatggtt gaagaaaatc gcgctcgcgg ctccgggaat ggcggattca
     2821 acgtccgcat catccaaaat aatgttgggc gatttcccgc cgagttcgag cgaaactttt
     2881 ttcagattgc cggcagctgc ttgcaaaatt aatttaccga cttccgtcga gccggtgaag
     2941 gcaaccttgt ccacgtccgg atgagcggca agagcggcgc ccgcggtttc tccgtaaccc
     3001 ggaacgatgt tcacgacgcc atcgggaatt ccggcctccg caaccaattc accaagcaac
     3061 agcgcggaga gaggcgtctg ctcggccggt ttcagaatga ccgtgcagcc tgtggcaaga
     3121 gcggggccga gtttccatgc ggccatcagc agcggaaaat tccacggaat aatctgcgcg
     3181 acgacgccca caggttcgcg aacggtatac gccagaaatt gtgtttttcc gccgcccgaa
     3241 gaaatcggaa ttgtgtttcc ttcgattttc gtggcccatc ccgccatgta gcgaaacaga
     3301 tcggctgcga gaggcacatc ggcgacgcgc gcgacggtga gaggctttcc gttgtcgaga
     3361 gattcgagct gcgcgaattc ttcgaggtgc tgctcgatta aatcgccgag cttccagatc
     3421 atgcggccgc gttgcgacgg cgtgattttg gaccacggcc cggtttcaaa tgcggcgcgg
     3481 gccgccttaa ctgcgcgatc tacatcttcc ttgtcgccct cagcgacctt ggataggact
     3541 tcgcccgtgg cgggattgta agtggggaaa gttttgcctg aggcggcatc cacccacttg
     3601 ccattaatta aaattttgtg agtcttggca ataaattgcg aaacatttcg atcgagtgac
     3661 ggcattggca cggttgtggc catatatatt ctccttctct acgaatcgac gtaggctcgt
     3721 gttatgaagg caaggcgaag tccacgctac tcccctcaat tgaaaaattc aagcagggga
     3781 ttggcagcag tagctcgcct ctgacccttc acgatatctc agggcaagag aggcggggta
     3841 cagcagtggg atgcgtttat agatgtgatt gaagatgttg aaaacttaac ggaagaaaat
     3901 tgcgatacac tcgacatgca agattcagtt ggacgggcgc ttccacggag atgaataaat
     3961 ggccggcgaa gatttgcgct acccgatcgg aaaatatgaa gcgaagagct tgctgacttc
     4021 tcccgagcga tcgctagcga ttgcgcaaat tgaggaaact ccgaaacgtc tttgcgaggc
     4081 ggttggccgt ctttccgttg gccaactcga cacgccgtat cgccccgatg gatggaccgt
     4141 gcgccaagtt gcgcatcacc ttccggacag ccacatgaat gcgtatgtgc ggttaaagct
     4201 cgcgctcacc gaaaatgaac ccattgtgaa accgtatgaa gaagcgcttt gggccgatct
     4261 tgccgattcg aaagaaacgc cagtcgaggt ctctctttct ttgctcgagt ttttgcatct
     4321 gcgttgggac agcttgctgc gcgcaatgca acctggtgat ttcgcgcacc ggctgcgaca
     4381 tcccgttaac ggggtgatga ctgtggacaa tctcgtgcac cactatgcct ggcatggacg
     4441 ccaccacgtt gcgcagatta cttcgttgcg cgaacgcgag gggtggaact agcccgtgat
     4501 atttgccgcg ctgcgctatt actggatcac ggccaaggga tacaggttgc gcccgtgggc
     4561 cagcccgtat cttcggtggc gcctcgaaac atttttcggc ggggacatgc acgcactcgg
     4621 agcggcggaa ttttttcggc tcggttggcg cgagcggaaa cggctcggcc gatttttggc
     4681 ctggacggag gagcggcggc gcgcgcaaag ggcagaaggc ctgcggtagg cggatcgggt
     4741 cgcgactctg gaatgcttgt gtcgccctga ccgggctttt tcccgcgcat cgatattttt
     4801 ccagcctgaa aacggtgggc tacgggattc cgtccctatg ggactttgct gttagattag
     4861 agcggtcagg gattgcacgt ctgaaggctg caatttttag atgctgcagc ttgcacatat
     4921 aaatcacaaa cctcaaatct caaatgccca attccaaaaa gccaccttgc ctgttgccgg
     4981 tgagatcccg atctcgtttc ctgaagagtc gcgctccttt tttgattttc agagcgcatt
     5041 tctaatagaa acgcaacgaa attcatttca cgaaaaggtg tatactggaa tcagattact
     5101 ctaagcaata aattgttagc gggacagaag tgccccccgg taggtcgcca tgaatgaatt
     5161 aaaaatattt caaaaaacag aatcgctgta tgaagggcgt tggtgggaac cgatgtcaga
     5221 cggccgcgtg cattgttttc tttgcccgag gcattgcc
//
LOCUS       DASZMB010000055        28930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2258842, whole genome shotgun sequence.
ACCESSION   DASZMB010000055 DASZMB010000000
VERSION     DASZMB010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 28930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28930
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2258842"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VG272_02345"
     CDS             complement(<1..183)
                     /locus_tag="VG272_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12548.1"
                     /translation="MNRRRFLHLLEVVAAGTIGAQFASCADSRELFAAPKSKRLFQPI
                     KGGRPQVAITMDDPSVD"
     gene            complement(355..1896)
                     /locus_tag="VG272_02350"
     CDS             complement(355..1896)
                     /locus_tag="VG272_02350"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HWF12549.1"
                     /translation="MADHPGSNSPQEQGPGTDAMLDGARYMASLRDTREVYIYGERVN
                     DVTQHPAFRNSCRSIARLYDALHDPKTRDTLTTVDRLSTRTHKFFKPCYSVQELCEAR
                     DAIVAWSRMSYGYMGRTPDYKAALMGSLASEPEFYAPFDESARKWYARYSAQCLFLNH
                     CLINPPIDRGRPVHEIGDVYVHMVKERDDGMVVSGAKMLGTGSAMTHATFVAQTNSTT
                     LDKEKAKDFAVVFIAPMNTPGVKLICRASYEQNAHSPFDYPLSSRFDENDAVLIFENA
                     FIPWENVLVYRDPERANGFYRHSRFVNRYTFQSSLRLAVKLDLMVGLFAKGIETNGTD
                     AFRGVQAALGEIIGWRNLMWGMTAAMCHDPEDAPGGALVPKQEYAYTMRLFCTQCWPA
                     VRHIFEKFLGGSLLMSPSSYKDFLNPDLQPLLEKYYRSASATAAERIKLFKLIWDAIG
                     TEFGARHELYEINYSGNHEQIRLDILSGAQKSGILDQCIALTNQCMSEYDLEGWKDST
                     WVWDK"
     gene            2147..2929
                     /locus_tag="VG272_02355"
     CDS             2147..2929
                     /locus_tag="VG272_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWF12550.1"
                     /translation="MRKFLRFNLHAAFIALFLLMPGGYLQADSSNHQSDVVRTSRGDL
                     RITPIYHGSVMLEFDGKVIHVDPWSKGDFTGFPPADLIVITHTHQDHLDRTMVDRLKK
                     PETIIVGPPAVIDTLNCAPACGIAEAISDSEHRTVMGIDIEGVPMYNIVFGSGPGKPY
                     HHKGIGSGYILTFGDTRIYFSGDTECTQEMKALKNISVAFLAMNPPRTETPADAAACV
                     KAFKPKIVYPYHYRGSNLEEFSAPLKDTPGVEVRIRKLEGEQ"
     gene            complement(3078..11198)
                     /locus_tag="VG272_02360"
     CDS             complement(3078..11198)
                     /locus_tag="VG272_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWF12551.1"
                     /translation="MANFPSERGAAFGCLNELLRVRAQEHGPRVAFRYLADGEAEEYT
                     LTYADLDLRARNIATVLTAQVSAGERALLFYTGGLEFISAFWGCVYAGVVAVPVFPAR
                     LHRQLPRLLSIAADSEAQLILTTAKIHRQSEDLFKRAPELKKLTWLATDDLPNASVEL
                     NDIGGDLERLAFLQYTSGSTAAPRGVMVTHGNLLHNLSCLREVFQFSEESVGVTWLPH
                     FHDMGLIGGLLQPVYAGGEMIVMPPSTFLQRPIRWLNAVTRYKATTMVAPNFAYDLCV
                     EKISAEQSESLDLGSVKVALCGAEPVRPDTLVQFSKAFVPCGFRQESFRPAYGLAEAT
                     LIVSGHSDGGAPFAPALQAEELRRNHVIPAEVGSVGSRTLVGCGGIAPGLNVKIIDPE
                     TRVICAKDRVGEIWVSGPSVALGYWHRDAETAETFGAHLATGEGPFLRTGDLGFLDQG
                     KLFVAGRLKDLIIIRGSNHYPQDLEHTVERSHRALRPTCGAAFSVDVEGAERLVIVQE
                     VNDRATASNEDIVAAIRQALAEADEVHPDAVVLIEPRTISRTSSGKIQRYACREAFIG
                     GTLEVVHEWRDLENQSDKNGNEANRPRSGLVWDYLSTQNYSHRLAGNASRNREQGAES
                     RQESTGCDPIAIVGMGCKFPGAAGLDAFWTMLRNGVDAISEIPRDRWDIDALFDPLPG
                     TTGKMSTRWGGFLSGVDQFDPHFFGISPREAASMDPQQRVLLEVAWEALENAGQPPDH
                     LAGTRTGVFVGIGGFDYSNVIINYKNHLQVMNAYLGTGNAHSIAANRISYLLDLRGPS
                     VAIDTACSSSLVAIHMACDSLRGAQSDLVIAGAVNLILSPEVTIAFSHARMMAADGRC
                     KTFDAKADGYVRAEGCGVVILKRLSDALRDRDNILGLIRGSAVNQDGRTAGIAAPNAS
                     AQQAVIRGALAQAGLAPKDLTYLEAHGTGTSIGDPIEVEAIKCVLGKTMPDDLPCLLG
                     SVKANIGHLENASGMASLGKVLMCLQHDEIPGQVHFSKLNPRISLEGTRLMIPSAPQP
                     WPSSPRRIAGISSFGFGGTNAHMIVEEAPPRIRADGLPDRPWHILALSARTEGAVKAL
                     AGRFENYLTQYPDENLADVCFTANAGRSHFARRIAVIAENKEQLRKSLASFAAGQPDS
                     RVVCELGVRHSTKKDGMRTAFLFGGEISSAGTVHQLYAAQPLFRAAMDRCSHFLGLHQ
                     HEPLLAALTPERKCSERRDVALFALEFALGKLWHSWGIEPDAVLGAGVGEYAAAVISE
                     VMCCEDALKLIVERARILRLAPDDNELKRMLDEFEAVASKINFQSPRVPFVSGLSGRQ
                     LGDREIPGANYWRLHVLQDTHLDEAMETLRAQSYDQFIEIGSSNHFSELDTNSNSKTT
                     AIFLPSLESNRNSWQTMLTSLASLYVRGANVDWKSFDEPYQPLKIALPTYPFERERCW
                     ESPPNTSFLQKSAKRFHSLLGERVNSALPMAQFQSRIGIGNPRYLGDHKVQGAVVLPA
                     AVYLEMAQAASTEILGPGPSILSNIAFQEALILPATGERTLQFVSTPASSGNASFQIY
                     SSNANLNGNHESTGWTLHASGDMRLEAASIARCTEAQYSIEEIQARCPRLKSGTELYA
                     SLKNAGLEYGPGFQGVQQVWCGYREALGAVSLLPQIHNSQNNGDSDGYWIHPALLDSC
                     LQVLGAALPEDKSGTGREWTYLPVGIASLRFAGRLFQRIFSHAVIGKESVLGAEFLEA
                     DIRLLGEDGCVLGELLGFRAKLVSGNEEREARENPDDLLYDTSWVEKRCAEGKSGNGL
                     QLVTWLIVADRGGVGQLLAERLQSQGKHCVVASPEKAHALLPSIKSVNVIHLSSLDIC
                     NSDAHSDTILDAQNQWTETFRFIQSLLKKSHGEKQRLWIVTRGAQSVGQPPAQVSVAQ
                     TPLWGLAKSIDLEHPELACKRIDLDPQAEDEELSALTKELSLSDEEREVAFRRGTRYV
                     PRLVHRARKASNGALMEIPDADSFRMEISRPGKLENLLLRASPRHGPNPGEVEIRVMA
                     AGLNFRDVMNAAGVYPGGPIPFGAECAGKISAVGAGVTDFKVGDDVLAVANASFGSYT
                     TADARAVVPKPPAFSFGQAATIPIAFLTVYYSLHYLAKLSRGERVLIHAAAGGVGLAA
                     VQLARHAGAEIFATAGSPEKRAYLKSMRVPHIMDSRSLNFADEIMRKTGGKGVDVVLN
                     SLPGEYITRSLSVLGAYGRFVEIGKTDIYQNKPLGLFPFRNNLSYFAVDLERVCRERP
                     DLLRSLLLELMAMFKQESLKPLPHNIFSVEDAANGFRYMARRKNIGKVVISFSSNESK
                     NHSRHAIAPRADATYLITGGHGGLGLSVARWLSEKGARQLVLLSRSGTSLAREELIGE
                     LKANGTEVATILGDVSNKSDVERALSEIRETMPPLRGIIHAAGVLDDHVFLKLEPESF
                     RRVTAPKVLGAWNLHLLTADLLLDFFVLFSSVASVLGSPGQANYAAANAFLDGLAEDR
                     RSRGLPGLSINWGPWAEVGMAARAATAHGPASRVMHPLAPAQALAVLDRLFAKNGPAK
                     AVAMSVDWALLVRSFNGQPPPSLLADLVREKGCPATSKVSRGSGPGFSTTELWAAPTT
                     QRHGLILAHVQKSLAQVMALESPELDPEESLSNLGLDSLMALELQHSLEESFSAKLPI
                     ELLMGMPSLNEFVNRLLEILAKTRSMPAQEISQEMFANDSKLTGILPPIVEA"
     gene            complement(11292..12059)
                     /locus_tag="VG272_02365"
     CDS             complement(11292..12059)
                     /locus_tag="VG272_02365"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HWF12552.1"
                     /translation="MNIAAFSIALCLFSALSVFAQDDAKLIMKRVYEQDTSRDVKLRA
                     TLEITDKDGHSVKKKFIISRIGSFGNGKTLVRFTDPTELRGVKLLSVNQPGEPDQQWI
                     YTPATERVRSITPRERAEHFAGSDFTYEDIAEHSLDNFTYELLPAEDVIENHKTFKIM
                     ATPTSPGNSQYKFIYYWVAQDVPCILHAEMYDQKGRKVREMRASGLRKESGIWGARKT
                     EMRSLLDGTKSVLMIDEAHFNTGLDSAMFTPESLEKN"
     gene            complement(12100..14661)
                     /locus_tag="VG272_02370"
     CDS             complement(12100..14661)
                     /locus_tag="VG272_02370"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWF12553.1"
                     /translation="MFGALWLITFKQWRAHKLRVGLTTLGIALGVAVFFAIRTGNATL
                     LDSLRSTVEKLAGKATLQISAGESGFSEKVLDTVRATPGVQLAEPVVETLVQTSFTDE
                     GALLILGVDTTGDQQLRDYQFDRTQTQISDPLVFLAQPNSILLSRSFADRHGLKVGNK
                     LSLFASDGEKEFTVQGTFKPVGVGNVFGGNIAVMDVYSAQVVFHRGRKFDRIDLMNAP
                     NVPVEVLQQRLRSELPPGVEVQRPEVRGQALENAVTAMRIGILITSFVALLVGVFIIF
                     NSFTIAVNQRWKEIGILRAVGVEQGNVTRMFLLEALVMGVIGSLAGIAGGFLLASAAN
                     HVMRGMVAAVYGVVATAAPARFHLDQCAIAFALGMAASVIGAWYPAQGAACLDPAMAL
                     HNVEARRREAVLGWRRIGGGLLLIVAGSALVMRTPPRMGLPIQFVFSTIVLLGLTLVL
                     PMLVFWSARAFRPIMNLLGGSEGALAVDAMIQTPRRCAATVGALMIGLMFVFSTASYI
                     QSYRQMIDRWMNQVLNADIFVATSAMLRSTSYHFSEDLGRRISELPGVMRVENVRFTA
                     IPYRGDTAAVIAIEMNGFLERSQSAIEGADSRTINGRLTKGQGVVVSRNFALRWGLGV
                     GSQLSLESPTGTLELPILGFLDDYRSEKGTIFMDRALYKKYWNDDAVDFVDVDLNPGV
                     DQMAMKKEVEKLTAGSFHAFVYTNAEFKRWISSLVDQFFTLNYMQLVVAVLIAVLGIV
                     NTLLISVAERRREIGIVRALGGLRSQIQKLVLLEAVMVAIVGVIVGSVAGILNTFFMS
                     HAVSVVLVGYTVPFYFPWKFILLSVPVVVVVSLTAGWWPARQAARMQVIEAIGYE"
     gene            complement(14661..15377)
                     /locus_tag="VG272_02375"
     CDS             complement(14661..15377)
                     /locus_tag="VG272_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF12554.1"
                     /translation="MIQMTNVSKTYGDQGTVKALINLSMTVEQGERVAVMGPSGSGKS
                     TLLNLICGLDQPTSGSIKLEGVELASLDDDHRTRLRREKLGMIFQTFNLLPTLTAIEN
                     AALPLRLQSLCCRESEQRAMAMLERVGLKVRAHHRPDELSGGERQRVAIARALIFRPP
                     ILLGDEPTGNLDSATGEEILRLLDDLHREYNNTILLVTHNDLAAEFCDRILTLRDGQF
                     VKETRTGRNERVGTAPAGEK"
     gene            15679..16215
                     /locus_tag="VG272_02380"
     CDS             15679..16215
                     /locus_tag="VG272_02380"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HWF12555.1"
                     /translation="MTLKILRNSAAAFVFTVLGTASMRAQGIVEYGVMTGTVAGAAAS
                     SKPLIPMPNFGLPVSTSPASPAVSGPAGAASMTPESAAKANVQFFQAHGGPTAAEIAV
                     RTVPDHASTWIDGKFVGPAPLTLKLAPGHHQILVRAVAMREYTQEFDLTAKQTQSIDV
                     ALKTSVQNQVVIHWPSQK"
     gene            16823..19342
                     /locus_tag="VG272_02385"
     CDS             16823..19342
                     /locus_tag="VG272_02385"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWF12556.1"
                     /translation="MLIATLFCAISSFAQQPGDTARSASGSIIGKVTSSTGQKGTDIL
                     SGITVKLTSTAPGSLHRTTLTDFEGHFEFTHLPQGSYNLEAVLEGFQSWTSTVSLGLG
                     EILTKDIPLQINSVEERIEVQGETTEIATESVSATATVTEQQLEELPLRTQKFTEALS
                     ISPSVIRTQEGKLNFNGQSESQGMLLVDSAENVDPVSGSFAIPIPVNTIQNIQVFTTP
                     DSSAYGGFSGGLTRIDIRPPAPAWNYKLLDIVPSFRGKNDHLIGLLNMTPGVEIGGPL
                     IKNKLNFSETMTYEFRKDPVHGLTWPWNETVTYSLVSFTEFQYTFSNKHVVNFNFNVF
                     PSTVLYANINALIPQTASANIRRRGSSVGISDAYQLDSGAVLTTLVRYTNFYNSEHGQ
                     GLADMTINPEGWGGNYFNTFSRNGNQMEALPILQLTTLSWLGSHQVQLGTDVLYRTFT
                     GSSVSRPINLLAQDGTIDETINFLGAGRLQSSDEEISGYAEDRWSLAKHMAFTMGGRV
                     THQTFGRNAAFAPRLGLSYSTPNGKIAVRAGAGLIYGHVPLLAADFAGYQERMITLNP
                     GTLAAQSYILQNVYQSPGMAGNSSTLTNSDASTHTFTWNVEAETSPRRDLSLRLGYYE
                     TRTTNLFVLNPIVPAGGGTSGFMALDNSGRSNYQQAQMTARYRPSERNEINISYAWSR
                     ARGDLNTVSDTFLPVQVPVIRPNLFGVRPSDIPNRVLAWGYITLPKKFVFSPVADVHS
                     GFPYSNVDNFQNYASVPNSLRFPIYFSLDVKLSREFTVRMPFKENAKRKKINIGVFSQ
                     DVTNRLNPHDVYNNVTAPFPLFGQFAGFQRRLTGLDINLTE"
     gene            19426..20259
                     /locus_tag="VG272_02390"
     CDS             19426..20259
                     /locus_tag="VG272_02390"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWF12557.1"
                     /translation="MPIAIGILCLPFFVATPVPSAFTAGDEQSLLQADHAAFESDSPS
                     PVLNDSLLDNNFTWTDSSGNTRNKSEILEAIRSKKKLVAEKGIVVAHAYRRVGIIQEN
                     AEKVYTLRIWIKRDGGWHLLIYQAVTIGAPPSAAADNEICENPCNTVPFHPRNNDERD
                     VIQTYQTVERAVTAHDSAAWGSHIADEFFAVTSNSNRPLDKATRMAGLDNQKVGGIAP
                     FPLVSAHMFAFGETMIMTSRQQPLHGLPLHVTRVWFKRNNTWVEAYSYQTTIQADVFP
                     R"
     gene            complement(20306..21085)
                     /locus_tag="VG272_02395"
     CDS             complement(20306..21085)
                     /locus_tag="VG272_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12558.1"
                     /translation="MLVALMILLVSLGSSVARAQSSLAQSAIEARLNLPQVVDKLVTK
                     NLERADALQKYQGRRTYTLAYSGFPASLHAEMVVDMTFDAPATKQFKIISQSGPQWLI
                     DRVLKRLLETEQEASTEENRARVALNSSNYDFSELARQDTPDNCSFVVAVTPKIPSKL
                     LYRGHIWVNSKDFAVCRIEAEPAKNPSFWIKKTDIHHSYIKIGDFWLPSENQSVSEIR
                     GGGHAILTIKYQNYEILAAHALKGSDPGPSSSSLASTEHSN"
     gene            complement(21166..22392)
                     /locus_tag="VG272_02400"
     CDS             complement(21166..22392)
                     /locus_tag="VG272_02400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF12559.1"
                     /translation="MTVENIRVALFPCTYREIDGVANTSKQFAEFARQHGIPFLLVHA
                     GPADEIVVDGSLTRIQLRRGRIKFPLDGHHEFDLLFLRHYQRVLRVVQDFGPNVIQIT
                     GPSDVGILGALLAHNLHVTLAASWQTNIHQFAGRRMSSLLSSWPKNLSRPLAAGAESA
                     SFRAAARFYKIPRLLFTPNRELVELLGKATGKPCFLMGHSVDTETFSPAFRERSAGPF
                     QIGYVGRLTPEKNVRFLAQLEKDLLAKGLKDFRIVLIGQGAEENWLRENMKHAEFKGW
                     LTGRDLSCVYADMDAFAFPSETDTFGLAVLEALASGVPAVVTSSGGPASTVKHGKSGY
                     VAKNFDEFAPLLMTLMAQPQLQKSMRLEARNRALAMGSWEQIFSGMYKTYERFAQPAA
                     QAEDMLLDDARKIMNT"
     gene            complement(22389..23543)
                     /locus_tag="VG272_02405"
     CDS             complement(22389..23543)
                     /locus_tag="VG272_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12560.1"
                     /translation="MPPSQISCFWKQPEIARQYRTAVSLHSHTNQSKESLLFIPAFAE
                     KRPLLRWALNEQIKKSVRPVDFSQAYWTPPLPPKLAYELESSQIEDNLELVSLVSLTD
                     HDSIEAPAILRQSPNPVQVPFAVEWSVPFEGAIFHLGIHNLPQSQAHLIMDDLSAYSR
                     NPSAERLVELLSSLHSIPDVLIVFNHPHWDQYCLGEATFRNFVDRFLQGYVEYIHAFE
                     LNAMRAWTENKSVIELAAAWQRPLISGGDRHGLEPSAALNLTCATTFSEFINEIRVEQ
                     LSHILFMPQYSELLGIRFMQTVIDTIRDYPDHPVGSRRWDERVFHMDFQVGYHRPLSC
                     LWKVPPSYLGHIFSIFRMIENTSVRHALNFTFREKAKVHRALDVPRGVSA"
     gene            complement(23863..24495)
                     /locus_tag="VG272_02410"
     CDS             complement(23863..24495)
                     /locus_tag="VG272_02410"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HWF12561.1"
                     /translation="MSRNTGLLRTAATLGLAIAALGFGSASAWAQGATPAPFNKVTVS
                     EVVAKRTLMKAVINADTARAITDACVDWQKGQPGNVSIAIFVLSPTGEIIDAHKMDGV
                     LPIGAETGLMKAKTALYARMPSALVAQRFNTVDSRVIRLDLGKEQGLAYYFVPGGLPI
                     VVDNQLIGAVGVGGGNADEMCAYQALTKVLGPQPPLTPPMPPAASGQPQR"
     gene            24863..25735
                     /locus_tag="VG272_02415"
     CDS             24863..25735
                     /locus_tag="VG272_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWF12562.1"
                     /translation="MTLPSERAKYSAIVDRPKLQLPNGERIVIWTIVNVEVWDIARPM
                     PRQVLVPPTGVTTLPDVPNWSWHEYGMRVGFWRFHALYERLKIRPSLSINARVCLDYP
                     RVAQACKDSGWEFMGHSFDQRPMHSEPDPVAMIERSVKTIQEFTGKSPIGWMGPGLTE
                     TYDTPDQLKAAGIKYICDWAWDDEPSEIATKHGPLYTLPYSLETNDIPVMAVQYHEAP
                     YWTQKCKDTFDRLYEEGANRPKIMAIAIHPYLSGQPSRIKYLENIYEYISKFTGVVHW
                     NGEQILEWYLKARK"
     gene            complement(25812..26354)
                     /locus_tag="VG272_02420"
     CDS             complement(25812..26354)
                     /locus_tag="VG272_02420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HWF12563.1"
                     /translation="MKVNSKIAPGYGFGRFAAVLILLLALPLAIGVKRAAAADDAANS
                     GEDAPIKQVVAGFSNGWNTHDAHSMCASLADNVDWVNWRGEPLGTRQAVEDEHARLFA
                     DLYKNTHRTDTVKTIHYISPELAVVDDYWSMTGARKRDGSDWPYRAGYSNFIMAKRNG
                     RWIIIISHTADFNAMAPAAK"
     gene            26599..26982
                     /locus_tag="VG272_02425"
     CDS             26599..26982
                     /locus_tag="VG272_02425"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HWF12564.1"
                     /translation="MKKRLLLVGFLLSCLLSAGPIFAHHSWSGYDSSNLTAVKGTVTQ
                     FEWGNPHIWINFEVHDDKGTIENWSAGGPSPNRLANTGWDKNTLKSGDQIAFVGNRIK
                     DGTYSMKLQKIVLPDGRELTCYRGR"
     gene            27222..27611
                     /locus_tag="VG272_02430"
     CDS             27222..27611
                     /locus_tag="VG272_02430"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HWF12565.1"
                     /translation="MRNRIGVMVLCIAILSVVSIQVFAHHGASPYDTSKLSTLKGTVS
                     DFQFINPHVEISVDVTNDKGKVETWIGEANSPNVLSRHGWDKDIIKKGDQITVIGNQG
                     KNGSKTLRLQKVILSNGKELDPNSITD"
     gene            27698..28492
                     /locus_tag="VG272_02435"
     CDS             27698..28492
                     /locus_tag="VG272_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12566.1"
                     /translation="MRNRINIWAFALNALLFFCSAHPAQLNAQQEPASAQPPATTPDL
                     SGLWSRLRDAAVPRGYGMYILDYGKTDSPMTPWAAAKYKTTSAMYHGDDPSTVLADPV
                     FQCFPPGVPRIYLFNFPVQIVQIPGQVLMLFEYDHFVRRIYTDGRPHDSEQGPLWMGD
                     SIGKWEGDTLVVDTTNFNDKTFIDRVGRPHSDALHVVERMRRVDQNSLEIDFTIDDPK
                     AYTKPWGTKLIFESKPTWKVLEQICEDNASFIGLNEKATQPAKKKK"
BASE COUNT         6999 a         7772 c         7507 g         6652 t
ORIGIN      
        1 gtcaacgctc gggtcatcca tcgtaatcgc cacctgcggt cgcccgccct tgatcggctg
       61 gaataatctt ttcgacttcg gagcggcaaa caactccctc gaatccgcgc aacttgcgaa
      121 ctgtgctccg atcgttcctg cagcgactac ttcgaggaga tggaggaatc gtcggcgatt
      181 catacgagga gtataaacgt caaaccgagc ggcgaagaaa aaaaccacca caggttgaca
      241 agatggcaac gtggcgcgat ctgtatgaag tcccggagag ttgatctcag tggataagtc
      301 ctgggaaaat gatgttcttg atgacgagat gttgcagtgt tgggcatttt cccactactt
      361 gtcccacacc caagttgaat ctttccagcc ttcgagatcg tactccgaca tgcactgatt
      421 ggtgagggcg atgcattgat cgaggattcc gcttttttgc gctccggaaa gaatgtcgag
      481 gcgaatttgc tcgtgattgc cggaataatt gatttcgtag agttcgtggc gggcgccgaa
      541 ttcggtgccg atagcgtccc agatgagttt aaaaagcttg atgcgctcgg cggcggtggc
      601 ggaggcactg cgataatatt tttcgaggag cggctgcaaa tcgggattca gaaaatcttt
      661 gtagctggaa ggcgacatga gcaggctgcc gcccagaaac ttttcaaaaa tgtggcggac
      721 cgccggccag cactgcgtgc agaataggcg catggtatag gcgtattcct gcttggggac
      781 gagcgctcca ccgggcgcgt cctccggatc gtggcacatc gccgccgtca ttccccacat
      841 tagatttctc cacccgataa tttcgccgag ggcagcctgg acgccgcgaa acgcgtccgt
      901 gccgttggtt tcgatcccct tggcaaaaag tcccaccatc aaatccagct tcacggccag
      961 gcgcaacgag gattgaaacg tgtaccgatt tacaaaacgc gagtgtcgat aaaaaccgtt
     1021 ggcgcgttcg ggatcgcgat agacgaggac attttcccac gggataaatg cgttctcaaa
     1081 aatgagcacg gcatcgtttt catcgaagcg gctggagagc gggtaatcga acgggctgtg
     1141 cgcattctgc tcataggaag cacggcaaat cagcttcacg cccggagtat tcatcggcgc
     1201 gatgaagacc accgcgaagt ctttcgcttt ttccttgtcc agcgtcgtgg aatttgtttg
     1261 tgcgacaaat gtggcgtgcg tcatggccga gcctgttccc agcattttgg ctccgctgac
     1321 gaccatgcca tcgtctctct cttttaccat gtgcacgtaa acgtcgccga tttcatgcac
     1381 ggggcggccg cggtcaatcg gcggattgat gaggcagtga ttcaggaaga ggcactgggc
     1441 ggaatagcgc gcgtaccatt tgcgagcgct ttcatcgaac ggcgcgtaaa actcaggttc
     1501 cgatgcgagg ctgcccatga gcgcggcctt gtagtccggc gtgcggccca tgtagccgta
     1561 gctcatccgc gaccatgcga cgatcgcatc gcgcgcctcg cagagttcct gtacggaata
     1621 acaaggcttg aaaaatttat gcgtgcgcgt gctcaatcga tcgacggtcg ttagagtgtc
     1681 acgcgttttc ggatcgtgga gtgcatcgta caagcgcgcg atagaacggc atgaattgcg
     1741 gaaagcaggg tgctgcgtga cgtcgttaac gcgctccccg taaatgtaga cttcacgtgt
     1801 gtctctcaag cttgccatgt accgcgcgcc atcgagcatc gcatccgtgc cgggaccttg
     1861 ttcttgaggg gaattgcttc cgggatggtc ggccatggcg cacttcctcc tcgaatgaag
     1921 acgtacacgt aatagatgga atgctatctt gagtcaagtt gaacggacag gatttggatt
     1981 tcgggagggg tttcgcgtca acgaatttat cacgccgtga ttttaaaaga aaaacttccg
     2041 gcgccaatga acgatttcgg gaaggtcttc gaccgtcatg aagtctttca acctatgcat
     2101 tgcggtgtat actttttgct aaatccagat ggggagaatt taattcatgc gcaaatttct
     2161 tagattcaat ttgcatgcgg catttatcgc actctttttg ttaatgccgg gcgggtatct
     2221 gcaggcagac agctccaatc atcagagcga tgtggttcgc acgtcacgag gggatcttcg
     2281 catcacgccc atttatcacg ggagcgtaat gctggaattc gacgggaaag tgattcacgt
     2341 cgatccgtgg agtaagggag attttacggg atttccgccg gctgacttga ttgtgatcac
     2401 gcatacgcat caggaccatc tcgaccgcac aatggtcgac agactgaaaa agccggagac
     2461 gatcattgtc gggccgcccg ccgtcattga tacgctgaat tgcgcgccgg cctgcggcat
     2521 tgcggaagcg atcagcgatt ccgaacacag gaccgtgatg gggatcgata tcgagggtgt
     2581 gcccatgtac aacattgttt tcggttcggg gccgggaaaa ccgtaccacc ataagggaat
     2641 tggcagcggc tacattttga cgtttggcga tacgcgtatt tatttctccg gcgatacgga
     2701 atgcacgcag gaaatgaaag ctctgaaaaa tatcagcgtt gcattcctgg cgatgaatcc
     2761 tccgcgaacg gaaactcccg ccgatgccgc ggcctgcgta aaagcgttca agccgaaaat
     2821 cgtgtatccc taccactatc ggggatcgaa cctagaagaa ttctcggcgc cgctgaagga
     2881 cacgcccgga gtggaagtgc gtatccggaa gctggaaggc gagcaataat ccggtcgcta
     2941 ttgcccggca tggtcatctt tgggatcaac acttgccagg tggtgaagtc gtaggattta
     3001 gttgcacagg cctcagcctt ggtggttagg ttgtgcagag acaaaaaagt ccacaggctg
     3061 aagcctgtgc tactcgatca cgcttccaca attggcggca agattcccgt taattttgaa
     3121 tcgttggcaa acatctcttg tgagatttcc tgcgctggca tcgaacgtgt cttggcgaga
     3181 atttccagca agcgattcac aaattcattc aagctgggca tgcccatcag caattcaatg
     3241 ggcagttttg cactgaacga ttcctccagc gagtgttgaa gttcgagcgc catgagcgaa
     3301 tcaaggccga gattgctcag cgattcttca ggatcgagtt ccggtgattc gagcgccatc
     3361 acttgtgcca aacttttctg aacgtgcgcc aggatcaatc catgccgctg cgtcgtagga
     3421 gcggcccaaa gttcggtagt tgaaaaacca ggccctgagc cgcgtgacac cttcgacgtg
     3481 gcggggcagc ctttttcccg aacgagatct gcgagtaaag aaggcggtgg ctgcccgttg
     3541 aacgatcgta ccagcaaagc ccaatccaca gacattgcta cggcttttgc ggggccgttt
     3601 tttgcgaaga gccggtcaag cacggctaat gcttgtgccg gcgcaagcgg atgcatcact
     3661 ctcgacgctg gtccgtgagc tgtggctgcg cgggcggcca tgcctacttc agcccaaggg
     3721 ccccagttga tggataatcc tggcaagcca cgagaacggc gatcttccgc aagcccatcg
     3781 aggaaagcgt tcgcggctgc gtagttcgcc tgaccgggcg aacccagcac ggaagccacg
     3841 gaagaaaaca ggacaaaaaa atcgagaagt aaatccgctg tcagtaaatg aagattccac
     3901 gcgccaagga ctttaggagc cgtgacgcgg cggaagctct ccggttcaag tttcagaaaa
     3961 acgtgatcgt ccagcactcc agcggcatgg atgattccac gaaggggcgg catcgtttcg
     4021 cgaatttcgg acagagcgcg ttccacatcg gatttattcg atacatcgcc tagaattgtt
     4081 gcgacttcgg tcccatttgc cttcaattca ccaatcaatt cttctcgggc caaggaagtt
     4141 ccactccggc tcaggaggac caattgccgc gcgccctttt cggacaacca tcgcgcgacg
     4201 gacagtccca gaccgccgtg ccctccggtg atcagataag tggcgtctgc gcgaggagca
     4261 atggcatgcc gcgaatgatt tttcgattcg ttgctgctga atgagatgac aacttttcca
     4321 atatttttcc gccgcgccat gtaccggaag ccgttggcgg catcttccac cgaaaaaata
     4381 ttgtgcggca acggtttcaa gctctcttgt ttaaacattg ccatcagctc aagtaagagc
     4441 gagcgaagca gatcgggccg ctcgcggcat acccgttcga ggtcgacggc aaaataggaa
     4501 agattattgc gaaacggaaa aagcccgagg ggcttgttct ggtagatatc tgttttgccg
     4561 atttcgacaa acctgccgta ggcaccgaga acggaaaggc tgcgcgttat gtattcgccg
     4621 ggcagagaat taagaacaac gtcgacgccc tttccgcctg tcttccgcat aatttcatcg
     4681 gcaaaattca aggagcgcga atccatgatg tgaggcacgc gcatggattt caggtaggcg
     4741 cgtttttccg gagagccagc ggttgcaaaa atttccgcgc cggcgtgccg cgcgagttgc
     4801 acggcggcaa gtcccacgcc gccagccgca gcatgaatca acacacgctc accgcgagaa
     4861 agttttgcga gataatgaag tgaatagtag acggtgagaa atgcaatcgg gattgtggcc
     4921 gcttgtccaa aactgaaggc gggcggtttg ggaacgacgg cccgcgcgtc ggctgttgtg
     4981 taagagccga aactggcatt cgccacggcg agcacatcgt cgccaacttt gaaatccgtt
     5041 acgcccgcgc ctactgccga gattttgcct gcgcattccg cgccgaatgg aatggggccg
     5101 ccgggataca ctccggcggc attcattaca tcgcgaaaat tcaggcccgc cgccattaca
     5161 cgaatttcaa cttcgcctgg atttggccca tgccgcgggc ttgctcgcag cagcaggttt
     5221 tccagttttc ctgggcgcga aatctccatg cgaaatgaat ctgcatcggg aatttccatg
     5281 agcgcgccat tcgaagcctt gcgcgctcga tggacaaggc gcggcacata tcgcgtccct
     5341 cttcgaaatg cgacttcccg ctcctcgtca gacagtgaca attcttttgt aagtgcggaa
     5401 agttcttcgt cttccgcttg cggatcgagg tctattcgct tacaggccag ttccggatgc
     5461 tcgagatcaa tcgatttcgc caatccccac aagggtgtct gcgcgacaga cacttgggca
     5521 ggcggctgtc caacggactg cgctccacgc gtcacgatcc acaggcgctg cttttctccg
     5581 tgagattttt taaggagaga ttgaatgaat cggaatgttt cggtccactg gttttgtgca
     5641 tcaagaatcg tatccgaatg ggcatccgaa ttgcagatat cgaggctcga aaggtggatg
     5701 acatttacgc tttttataga aggcaggaga gcatgagcct tttctggact cgcgacaacg
     5761 caatgctttc cttgcgattg aaggcgctct gcgagcaatt gtccgacgcc tccacgatcg
     5821 gcaacaatca gccaggtaac gagctgcaga ccatttccgg attttccttc tgcgcaccgc
     5881 ttttctaccc agctagtgtc atacagcaga tcatcaggat tctcgcgagc ttcgcgttcc
     5941 tcatttccgg aaacaagctt tgcgcgaaaa cccaaaagtt ctccgagaac acatccatct
     6001 tcgccaagca aacgaatatc cgcttcaaga aattccgctc caagaacact ttctttcccg
     6061 atgacagcgt gactaaaaat tcgctggaac agccgtcctg caaaccgaag actggcgatt
     6121 ccgacaggaa gatacgtcca ttcgcgacca gttccgcttt tatcttcagg caaagctgca
     6181 cccagcacct gcaagcagga atcgagcagc gcgggatgaa tccagtagcc gtcagaatct
     6241 ccattgtttt gagaattatg aatttgcggt agaagcgaaa cagctcctaa tgcttcccga
     6301 taaccgcacc atacttgctg gactccctga aaacctggcc catattccaa tcctgcattt
     6361 ttcaaggacg cgtaaagctc cgttccactt tttaggcgcg ggcatcgagc ttgaatttct
     6421 tcaatgctgt attgcgcttc cgtgcagcgg gcaatacttg ccgcttcaag tcgcatgtcg
     6481 ccggaagcgt gcaaagtcca tcccgtgctt tcgtgattgc cgttcaagtt tgcattggaa
     6541 ctataaattt ggaaggaagc attgccactc gatgccggag tggaaacgaa ttgcaaagtg
     6601 cgttcacccg tcgcgggaag aattagcgct tcctgaaatg caatattgga taatatcgag
     6661 gggcccgggc caagaatttc cgtcgaggcc gcctgagcca tctccaaata aacagcagcc
     6721 ggtaaaacaa cagcaccctg aactttgtgg tcgcctagat agcgaggatt tccgattccg
     6781 attctcgatt ggaattgcgc catgggcaaa gcggaattca cgcgttcacc caggagagaa
     6841 tgaaatctct ttgccgattt ttgcaaaaag gacgtattgg gcgggctttc ccagcagcgc
     6901 tcgcgctcga atggataggt cggcagagct atcttcagcg gctggtacgg ctcatcgaaa
     6961 gatttccagt ccacatttgc gccccgcacg tataacgaag ccagactcgt gagcatggtc
     7021 tgccaactgt tgcgattgct ctcgagcgaa ggcagaaaaa tggcggttgt tttcgagttg
     7081 ctgttcgtat ccaattcgga aaagtgattg gacgatccga tctctataaa ttgatcgtag
     7141 ctttgcgccc gcagagtttc cattgcctca tcgagatgcg tgtcttgcag cacatgaagc
     7201 cgccagtagt ttgctcctgg aatttcgcgg tcccccaatt ggcgcccgct aagccctgaa
     7261 acaaacggaa ccctgggcga ttgaaagttt attttggatg ccactgcttc aaactcatcg
     7321 agcattcgct ttaattcgtt atcgtcagga gccagtcgca aaatgcgtgc gcgctcgaca
     7381 atcaatttca gagcgtcctc gcagcacata acttccgaga ttaccgctgc cgcatattca
     7441 ccaacgcccg ctcccaacac agcgtccggt tcgatgcccc aggagtgcca aagctttcca
     7501 agggcaaatt caagcgcgaa tagtgccacg tcacgacgtt cggaacattt ccgttcaggg
     7561 gttagcgctg cgagtagcgg ttcatgttgg tgcaaaccaa gaaaatggga acagcgatcc
     7621 attgccgcgc gaaacagcgg ttgcgcagcg taaagttgat gaaccgttcc ggcactagaa
     7681 atctctcctc cgaataaaaa tgcggtgcgc atcccgtctt tcttagtgga atgcctgacg
     7741 cccaattcgc atacgacacg cgaatccggt tgtcccgcgg caaaagaggc aagcgatttt
     7801 cgaagctgct ctttattttc ggcaatcacg gctatgcgcc gcgcaaagtg ggatctgccg
     7861 gcattggcag tgaaacaaac atcagcgaga ttttcgtcag gatattgcgt taaataattt
     7921 tcaaatcgac cagcgagcgc tttgaccgcg ccttccgtgc gcgcagacag cgccaaaata
     7981 tgccagggac gatccggcaa tccgtccgcg cgaattctcg gcggcgcttc ttcgacaatc
     8041 atgtgggcgt tcgtcccgcc aaatccgaaa ctgctgatgc ccgcgatgcg ccgaggcgaa
     8101 gaaggccagg gttgaggcgc gctgggaatc atcaagcgag ttccctcaag cgaaatgcga
     8161 ggattaagtt tggagaaatg cacctggcca ggaatttcat cgtgctgcaa acacatcagc
     8221 accttgccca ggctggccat gcccgatgcg ttttccagat gaccgatatt ggccttcacc
     8281 gaaccgagca gacatggaag gtcatccggc atcgttttcc caagcacgca tttaatcgcc
     8341 tcaacctcaa tcgggtcgcc aatcgaagtg ccggtgccat gggcttccaa gtaggttaaa
     8401 tcttttggag caagtccagc ttgcgcaagt gctccgcgaa tcaccgcttg ctgagccgat
     8461 gcgttgggag cagcgatgcc cgcagtgcgt ccatcctgat tgacggccga accccgaata
     8521 agcccgagaa tattatcgcg atcacgcaac gcatcggaga gccgcttaag gataacgacc
     8581 ccgcagccct ccgcgcgaac gtaaccatca gctttggcgt caaatgtttt gcagcggcca
     8641 tcggcggcca tcatgcgagc gtgcgaaaaa gcgatggtaa cttcgggaga caaaatgaga
     8701 ttgacggcgc cggcgatcac caaatcgctc tgtgcaccgc gcaaactatc gcaggccatg
     8761 tgaatggcga cgagcgaaga cgaacaagct gtatcaatgg ctacactggg accgcgcaaa
     8821 tccagaaggt aagaaattcg attcgcagca atgctgtgcg cgttacctgt cccaagatac
     8881 gcgttcatta cttgcaagtg gtttttgtaa ttaattatga cattggagta atcgaatcca
     8941 ccgatgccga cgaacactcc ggtgcgcgtt cctgccaaat ggtccggggg ctgccctgcg
     9001 ttttccaaag cttcccaagc cacttcgagc aaaacacgct gttgcgggtc catggacgca
     9061 gcttcgcgcg gcgaaatacc aaaaaaatgg ggatcgaact ggtcaacgcc ggaaagaaag
     9121 ccgccccatc gcgtgctcat tttcccggtg gtgccgggaa gcggatcgaa cagcgcgtca
     9181 atgtcccagc ggtcacgcgg aatttctgaa atggcatcca caccatttcg tagcatggtc
     9241 caaaatgcat cgagcccagc ggcgcctgga aatttacatc ccatgccaac gattgcaatt
     9301 ggatcgcagc ctgtgctttc ctgccgtgat tctgcgccct gttcgcgatt tcggctggcg
     9361 ttgcccgcaa gtcgatgtga ataattctgc gtgctcaggt aatcccaaac gagtccgcta
     9421 cgtggacggt tcgcttcatt cccatttttg tcggattgat tttcgaggtc acgccattca
     9481 tgcacaactt caagcgtgcc accgataaac gcttcgcggc aggcgtagcg ctgaattttt
     9541 ccgctagagg tccttgaaat cgtgcgcggc tcgatcagta ccaccgcgtc aggatgaact
     9601 tcgtcggctt cggcgagggc ctggcgtatt gccgcgacga tatcttcatt ggaggcagtt
     9661 gcccggtcat ttacttcctg cacgattact aatcgttccg caccttcgac atcaaccgaa
     9721 aatgcagcgc cgcaagttgg ccgcagcgca cgatggcttc tctcgacggt gtgttccaaa
     9781 tcctgcggat aatgattaga accacgaatg atgatgagat cttttagtcg ccctgcgacg
     9841 aacagctttc cttgatcgag aaatccgaga tcccccgtgc gtaagaaagg accttcgccc
     9901 gtggctagat gcgcgccaaa ggtttcggca gtttccgcat ctctgtgcca ataaccaagc
     9961 gcgacgctgg ggcctgaaac ccatatttcc cccacgcgat ctttcgcaca tatcacacga
    10021 gtttcagggt cgataatttt tacgttgagg cccggcgcaa ttccgccgca gccaaccaga
    10081 gtacggcttc ccacagagcc aacctccgca gggataacgt gattgcgtcg cagctcttcg
    10141 gcttgcaggg ccggcgcaaa gggtgcgccg ccatccgaat gtccggaaac gatgagtgta
    10201 gcttccgcca aaccgtaagc aggcctaaac gattcttgcc ggaaaccaca gggaacgaaa
    10261 gccttgctga actgcaccag cgtatccggg cgcacaggct cagcaccgca gagcgccact
    10321 tttacactgc caagatcgag cgactcggat tgttcagcgg aaattttttc gacacacaaa
    10381 tcgtaggcaa aattcggagc aaccatggtg gtcgctttat accgcgtgac ggcattcagc
    10441 caacgaatgg gcctttgcaa gaaagtggat ggcggcatca cgatcatttc gccgcccgca
    10501 taaaccggct gcagcaaccc gccgatcaaa cccatgtcat gaaagtgtgg caaccaggtg
    10561 actccgacgc tttcctctga aaactgaaat acttcacgca gacacgaaag gttatgcaga
    10621 agattgccgt gtgtcaccat tactccgcgg ggagcagcgg tggaacctga tgtgtactgc
    10681 agaaatgcca gtctttcgag atccccgcca atgtcgttca attccactga ggcattcggc
    10741 agatcgtcag ttgcaagcca cgtaagtttc ttcagttcgg gcgcacgctt gaataaatct
    10801 tccgactgtc gatgaatttt tgccgtggtc aaaatcagct gggcttcgga atcagcggca
    10861 atcgacagca gtcgtggcaa ctgcctatga aggcgcgcgg gaaaaaccgg caccgcgaca
    10921 acgccagcgt agacgcaacc ccagaacgcg gaaataaatt cgagcccgcc tgtgtaaaaa
    10981 agcagagcac gttcacccgc agaaacttgg gccgtgagta cggtagcaat gttccgggcg
    11041 cgcaaatcca gatctgcata ggtgagggtg tattcttcgg cctcgccgtc ggcaaggtaa
    11101 cggaaagcga cgcgcgggcc gtgctcttgc gcccttacac gaagaagttc gtttaaacaa
    11161 ccgaaggccg cgccccgttc ggatggaaag ttagccaaag ttcaaaaccc cccacaagtt
    11221 gccgggcagg acggaagcgc ggtgaatacc cggagattca tagacctgtt ttctgcttcc
    11281 tatcattgtt actaattttt ctccagcgat tccggagtga acatggcgct gtccaatccg
    11341 gtattgaagt gggcttcatc gatcattaag accgatttcg tgccatcaag cagggagcgc
    11401 atttccgttt tgcgtgcgcc ccaaatgccg gactctttgc gaaggccgct ggcacgcatc
    11461 tcacgaactt tgcgcccctt ctgatcgtac atctcggcgt gcaggatgca gggtacatcc
    11521 tgggccaccc agtaatatat aaatttatat tgcgaattcc cgggcgacgt tggcgtagcc
    11581 ataatcttga aagtcttatg attttcgatc acatcttcag cgggcaacaa ttcataggtg
    11641 aaattatcca atgagtgttc ggcaatatcc tcatatgtga aatcactgcc ggcaaaatgc
    11701 tcggcccttt cccgaggagt gatgctgcgg accctttccg ttgcgggcgt gtagatccat
    11761 tgctggtcag gctcgcctgg ctgattgacg gacagaagct taacgccgcg cagttccgta
    11821 ggatcggtga agcggaccaa tgtcttgcca ttcccaaagg aaccgatacg gctgatgatg
    11881 aacttctttt tcaccgaatg gccatctttg tccgtaatct ccagtgtggc tcgcaacttc
    11941 acgtcgcggc tggtgtcctg ctcgtagacg cgcttcatga ttaatttggc gtcgtcctgg
    12001 gcaaaaacgg aaagtgcgct gaacaaacaa agcgcgatgg agaacgccgc tatgttcaag
    12061 cgccttccgt gcctttgctg caataatgct acagctgaat cattcatagc cgattgcctc
    12121 aataacctgc attcgtgctg cctgccgtgc gggccaccag ccggcagtga gcgacacgac
    12181 gaccacaacg ggaacagata acagaataaa tttccatgga aaataaaatg gcacggtata
    12241 gccaacgagc actacggaca cggcatgcga catgaaaaaa gtattcaaaa ttcccgccac
    12301 cgatccgacg attacgccga cgattgcaac catcacggct tccagcagaa caagtttttg
    12361 aatttgcgat ctcaagccgc ccagtgcgcg cacaattcca atttcacgac gccgttccgc
    12421 cactgatatc agcagggtgt tcacaattcc aaggaccgcg atcaatacgg cgacgacaag
    12481 ttgcatgtaa ttgagcgtaa aaaactgatc caccagactg gaaatccatc gcttgaactc
    12541 ggcattcgta taaacaaatg cgtggaacga accggcggtt aatttttcca cttctttttt
    12601 catcgccatt tgatcgacgc ctggattcaa gtcgacgtcc acgaaatcca cggcatcgtc
    12661 gttccagtat tttttataaa gggcgcggtc cataaagatg gtgccttttt cagaacggta
    12721 gtcatcgaga aaaccgagga tcggaagttc cagcgtccca gtgggcgatt ccaggctgag
    12781 ctgacttccc actccgaggc cccaacgcag ggcgaaattt ctggacacca ccacgccctg
    12841 gcctttcgtc aaccggccat tgatggtgcg tgaatccgct ccttcaatgg cgctttgcga
    12901 cctctccagg aacccattca tttcgatggc aatcacggcc gccgtgtcgc ctcgatacgg
    12961 aatggcggta aaacgcacgt tttcgacgcg catcactccg ggaagttccg agatgcgccg
    13021 tcccaaatct tcgctgaaat gataactggt ggaccgcagc atcgctgaag tcgcaacgaa
    13081 aatatccgcg ttgagcacct gattcatcca gcgatcgatc atctgtctgt aactttgaat
    13141 gtaagaggcc gtggaaaaaa cgaacatcaa tccaatcatg agcgcgccaa ctgtggccgc
    13201 gcatcttcgc ggcgtctgaa tcatggcatc gacggccagt gcgccttcgg accctccaag
    13261 caaattcatg atggggcgaa atgcgcgcgc cgaccagaag accaacattg gcagcacaag
    13321 cgtgagcccc aacagcacaa tggttgaaaa aacaaattga ataggcaggc ccattcgcgg
    13381 gggagttctc atcaccagtg cggaacctgc gacgattagg agcaatccgc caccgattcg
    13441 cctccaaccc aaaaccgctt cgcgacggcg cgcttcaaca ttgtgcaacg ccatggcagg
    13501 atcgaggcaa gcagcgccct gcgccggata ccatgcaccg atcacggacg cggccattcc
    13561 cagcgcaaag gcaatcgcgc actgatccag atggaatcgc gcgggagcag ccgtggccac
    13621 aacgccgtag accgcagcca ccatgcctcg catcacatga ttggccgccg acgcgagcag
    13681 gaatcctcct gcaatcccgg cgagcgatcc gatcacgccc atgaccagcg cttccaacaa
    13741 gaacatgcgg gtcacatttc cctgctccac acctacggcg cgcaaaatcc caatttcttt
    13801 ccagcgctga ttgacggcga tggtgaagga attaaaaata atgaaaacac ctacgagcag
    13861 tgcgacaaaa ctcgtaatca gaatcccaat gcgcatggcg gtcacggcgt tttcaagagc
    13921 ctgtccgcgc acttcgggac gctggacttc cacgcccggc ggaagttccg agcgcagtcg
    13981 ttgctgcaat acttccacag gaacattggg ggcattcatc aagtcaatcc ggtcaaactt
    14041 gcgcccgcga tgaaatacca cctgcgccga atacacatcc atcacggcaa tgtttccgcc
    14101 aaagacgttt ccgacgccaa ccggcttgaa agttccctgt acggtaaatt ccttttcccc
    14161 gtccgaagca aaaagcgaaa gcttgtttcc cactttcaaa ccgtgacggt cggcaaagga
    14221 acgcgagaga agaatggaat tcggttgcgc gagaaacacg agtggatcgg aaatttgcgt
    14281 ttgcgtgcga tcaaactgat agtcccgcag ttgctgatct ccggtcgtat ccacgcctaa
    14341 gatcagcaga gcgccttcgt ccgtaaacga cgtttgcacc aacgtctcga ccacaggttc
    14401 ggcgagttgt acgccgggag tcgcgcggac ggtgtcgagg actttttctg aaaatccaga
    14461 ttctcccgcg gaaatttgga gtgtcgcttt gcccgccaat ttttccacgg tagagcgaag
    14521 cgaatcaagc agagtggcat tcccggtgcg aatcgcaaaa aatacggcca cgcccagcgc
    14581 tatgccgagt gttgttagtc ccacgcgcag cttgtgcgct cgccattgct tgaaagtaat
    14641 tagccacagc gcaccgaaca ttatttttct cccgctggcg cggttccaac ccgttcgttg
    14701 cgtcccgtgc gcgtttcctt cacgaattgg ccgtcccgca gagtaagaat acgatcgcaa
    14761 aactcggcgg caagatcatt gtgtgtgact aacaagatgg tgttgttata ttcgcggtgc
    14821 aaatcgtcga gcaggcgaag gatttcctcc cccgtggcgg aatcgagatt tcccgttggc
    14881 tcatccccca gcaaaatggg cggccgaaaa atgagagcgc gtgcaatcgc cacgcgctgg
    14941 cgttcgcctc ccgatagttc atcagggcgg tgatgcgcgc ggactttgag cccgacacgt
    15001 tccaacatag ccattgcgcg ctgttccgat tcgcggcagc acaggctttg caacctcagc
    15061 ggcagagccg cattttcaat ggcggtcagc gtgggaagca gattaaacgt ttgaaaaatc
    15121 atcccaagtt tttcgcggcg aagccgcgtt cgatggtcgt catccaggga agccagctcg
    15181 acgccttcca gtttgatgga accggacgtc ggctgatcca aaccgcaaat aaggttgagc
    15241 agcgtcgact tcccagagcc ggaaggaccc atcaccgcta ctcgctcgcc ctgttcgacg
    15301 gtcatgctca aattgattag agcctttacc gtaccctggt ctccatacgt cttggagaca
    15361 tttgtcattt gaatcatagt tttggccgtg aatgtccgga attggcgggc ctgttcctca
    15421 agcagaatat gattctaccc cagccccgga gaagcgaata gctggaattt caggttccca
    15481 ataaagcgcc ggtttcctca aatctttgtg atgcaattca tccagatgga agagtgcctt
    15541 gcaggataat tcctactcct tttgaagcct ggcgggcgct ctcgaatggg cagctggagg
    15601 aaaagaaacc ggttgccgct aattgcaagt gggactatga ttcctctcgt cccccacctt
    15661 gctttgtagg agtttgcaat gacgctcaag attctgcgta attctgcagc cgcattcgta
    15721 ttcacggttt tggggacagc ctcgatgcgc gcacaaggga ttgtggaata tggagttatg
    15781 accggaaccg tggcaggcgc tgccgcttca agcaagccgt tgatccccat gccgaatttc
    15841 gggctgccgg ttagcacctc gccggcctca ccagccgtgt ctggccctgc gggcgcagcc
    15901 tcaatgacgc ccgaatctgc ggcaaaagca aatgtacaat ttttccaggc tcacggcgga
    15961 ccaacggcgg cagagattgc cgtgcgcacg gttcccgacc atgcctcgac ttggatcgac
    16021 ggcaaattcg tcggacccgc tcccctcacc ttgaagcttg cgcccggcca tcatcaaatc
    16081 ctggttcgcg cggtcgccat gcgtgagtac acgcaagaat tcgacctgac ggcgaagcag
    16141 acgcagtcca tcgacgtggc actgaagacc tccgttcaaa atcaagtggt catccactgg
    16201 ccatcgcaga aatagactaa cgaaccggag tagcttttct tcttgatgcc gtgcaaagtc
    16261 acatggacga agtttgcact cgtctagcta tccgctcata ttccgcgaaa tctgaaatcc
    16321 ccagacggaa aaacgacttt ttgccggact tccgcccacg ctttttttgc gttccttgtg
    16381 acggctaacc ttgcctaaat gacctttttg ctttcactat gcgccggatt gagcatcagt
    16441 aaccctttgc gaaagttgtc ccaacctgac acttggagcc gggcaagaaa aaagagagca
    16501 tgaagagcaa acattcctca tgtgcctgca gagaaaacac cgccgctgcg tgtgtgaagg
    16561 gatatagtgt ggcacacctc cagcgggatt ttgagtttgt ccgtcacgtt tcggtttttt
    16621 gagcgtcact gaatgccacg gccggatgaa gcatttcgcg acagacagat gaattgaatt
    16681 gcgcgaggca gcaccacttg cgcagcaagc gtttcggccc agcattgcca cgtcgaggtc
    16741 ttctcgtacg aaagtgtgaa gcatgcagtg gaacgttcgt tgcaaaagta gggattgcat
    16801 cagaaaccgg attcgcgcag ctatgctgat tgcgaccctg ttctgcgcga tttcctcctt
    16861 tgcacaacaa cctggagaca cggcacggtc cgcttccggt tccataattg gaaaagttac
    16921 ctcctccaca ggacaaaaag ggactgatat tctttccgga attacggtca agctcacatc
    16981 taccgctccg ggttcgttgc atcgcacgac actcaccgat tttgaggggc atttcgaatt
    17041 cacgcacctg ccgcaaggca gctataacct cgaagcggtc ctcgaaggat ttcagtcgtg
    17101 gacctctact gttagcctcg ggcttgggga gattctcacc aaagatattc ctttgcagat
    17161 taattccgtg gaagaacgca ttgaagtcca aggcgaaaca accgaaatcg ccacggaaag
    17221 tgtatccgcc acggccacgg tcactgagca gcaactcgaa gaactcccac tgcgaacgca
    17281 aaaattcacg gaagcgcttt ccatttcgcc cagcgtgatt cgcacgcagg aaggaaagct
    17341 gaatttcaat ggtcaatcgg aaagccaggg aatgctgctg gtcgattccg cggaaaatgt
    17401 ggatcctgta tcaggaagtt tcgcaattcc catccctgta aatacgattc aaaacattca
    17461 ggtttttacc acgccggaca gttccgcgta cggtgggttt tccggcggat tgaccagaat
    17521 cgatattcgg cccccggcgc ccgcgtggaa ttacaagcta ttggatattg ttccgtcttt
    17581 tcgcggtaag aatgaccact tgatcggctt gttgaacatg actccgggag tggaaatcgg
    17641 cggcccactc atcaagaata aactcaattt ttctgaaacc atgacttacg aatttcgcaa
    17701 agatcccgtg cacggcctta cctggccctg gaacgaaacg gtcacataca gcctggtgtc
    17761 ttttacggaa tttcaatata cgttttccaa caagcacgtg gtgaacttta attttaatgt
    17821 ttttccatcg acggtcttgt acgcgaatat taatgcgctt attccacaaa cagcttcggc
    17881 aaatattcgg cgcagagggt cgtccgtagg aatatccgat gcataccagt tggattccgg
    17941 cgcggttctg accacgctgg ttcgctacac gaatttttac aatagcgaac acggccaagg
    18001 gctggccgac atgacgatca atcccgaagg ctggggcggc aattatttca atacattttc
    18061 cagaaatggc aatcagatgg aggctcttcc aattcttcag ctgaccacgc tctcatggct
    18121 gggcagtcat caagtgcaac tcggaaccga cgttctctac cgaactttta ccggaagcag
    18181 cgtctcccgc ccgataaacc tgctcgcgca ggacggtaca atcgacgaaa caatcaattt
    18241 tctgggagcg ggaaggctgc aaagttccga cgaagaaatt tcaggatatg cggaagatcg
    18301 ctggtcgctc gcgaagcata tggcattcac catgggtggg agagtaactc atcaaacctt
    18361 cggccggaat gctgcgttcg cgcctcggtt gggcctgtcg tattcaacgc cgaatggaaa
    18421 aattgcggtc cgcgccgggg ctgggttgat ctatgggcac gtgccattgc ttgccgcgga
    18481 ttttgcgggc tatcaggaac gaatgatcac cctcaatccg ggaactctag ccgcacagag
    18541 ttatatatta caaaatgtgt accaatcgcc gggtatggcg gggaattctt caacattaac
    18601 caacagcgat gccagcacac acacgtttac ctggaacgtc gaagccgaaa cttctccgcg
    18661 gcgggatctt agcctgcgac tcggttatta cgaaacccgg actacaaatc tcttcgtttt
    18721 gaatccaatt gttcctgcgg gcggcgggac ttccggtttt atggcactgg ataattcggg
    18781 aagatcgaac taccagcagg ctcagatgac cgcccgctac cgccccagcg aacgaaacga
    18841 aattaatatt tcctacgcgt ggagccgtgc gcgcggagac ctcaacacag tttccgatac
    18901 tttccttccg gttcaagtgc ctgtcattcg gcccaattta tttggagtgc ggccctctga
    18961 tattccgaac agggtgctgg cctggggcta tatcacgttg cccaaaaaat ttgtcttcag
    19021 ccccgtggcg gacgttcaca gcggtttccc ttattccaat gtcgataatt ttcaaaatta
    19081 tgcgagtgtt ccgaatagtt tgcggttccc gatttacttt tcgctggacg taaaactttc
    19141 ccgggagttt acggtccgca tgcccttcaa ggaaaatgcc aagcgaaaaa aaatcaatat
    19201 tggagttttt tctcaggatg taacaaatcg cttgaacccg cacgatgttt ataacaatgt
    19261 gaccgctcct tttcccctat tcggacagtt tgccgggttc cagcgtcgtt tgacggggct
    19321 cgacatcaat ctcaccgaat gattgcttaa cgactggtgg cgaatgaagt tgtagaatta
    19381 tcccgcatag catccgcact caaggatctc cgcgtgaagc tctcgttgcc aattgccatt
    19441 ggaatattgt gcttaccctt ttttgttgca acccccgttc catctgcgtt taccgctggc
    19501 gacgaacaat ctttacttca ggcggaccat gcagcttttg aatccgatag cccttcgccc
    19561 gttttgaacg atagtttgct ggataacaat tttacgtgga cggattcctc cggtaatact
    19621 cgaaacaaat cggaaatcct ggaagccatc aggtccaaaa agaaattggt tgctgaaaaa
    19681 ggtattgtcg tcgcccatgc ctacaggcgg gtggggatta tccaggaaaa tgctgaaaaa
    19741 gtttacacat tgcgcatctg gatcaagcgc gatgggggtt ggcacttact gatttatcag
    19801 gccgtcacga ttggcgctcc tccctccgcc gcggcggaca atgaaatttg tgaaaatccc
    19861 tgcaacacgg tgccattcca tccgcgaaac aacgacgagc gcgatgtcat tcaaacgtat
    19921 caaacagttg aacgagcggt aaccgcgcac gattctgctg cgtggggctc tcatattgct
    19981 gatgaatttt ttgccgtcac atcaaacagc aatcgtcctt tggataaagc gacgcgaatg
    20041 gcgggcctgg acaatcagaa ggttggggga attgcgccat tcccacttgt gtcagcacac
    20101 atgtttgcat tcggcgaaac gatgatcatg acttcacgcc agcaaccgct ccatggattg
    20161 ccgctgcatg tcacgcgagt gtggttcaag cgcaacaaca catgggtcga ggcctacagc
    20221 taccagacaa cgatccaggc agatgttttc ccccgataag ctcgcaaaga aaaactggca
    20281 cgaaaattct atttaattta ccggtttagt tggaatgttc ggtactagct agagagctgg
    20341 acgaagggcc aggatcgctt cccttgaggg catgcgccgc gagaatttca taattctggt
    20401 atttgatcgt cagaattgcg tggccgccac cgcgtatttc tgagaccgat tgattttcgg
    20461 aaggcaacca gaaatccccg atcttgatat aggaatgatg aatgtccgtc ttcttgatcc
    20521 agaacgacgg attcttggca ggctccgctt caatcctgca cacggcgaaa tccttgctat
    20581 ttacccagat atgcccgcga taaagaagct tactgggaat ttttggcgtt accgcaacca
    20641 cgaaggaaca gttgtccggt gtgtcctggc gcgcgagttc gctgaaatcg taattgctgg
    20701 agttaagcgc aacacgagca cgattttctt cagtcgaagc ttcctgctcg gtttccaaga
    20761 ggcgtttgag aacccggtca atcagccatt gcggaccgct ctgcgaaata atcttaaatt
    20821 gtttggtagc gggcgcatcg aacgtcatat ccacaaccat ctcggcgtgc agtgatgcgg
    20881 ggaagccgga atacgccaag gtgtaagtgc gccggccttg atatttctgc aacgcgtccg
    20941 cgcgttccag gtttttcgtg accagcttgt cgacgacctg cggtagattc aatctggctt
    21001 cgatagccga ctgagccaaa gaggattggg cgcgagccac agacgacccc aaagagacca
    21061 acaaaatcat tagagcgacc agcattctga aaaaacgttt tccaatggac ataaaatctt
    21121 ctagcctctc cggcgactgt gtgtagccgg gaatattatt tgaactcaag tgttcatgat
    21181 ctttcttgca tcatcgagga gcatgtcttc tgcctgagca gcgggctgcg caaatcgctc
    21241 gtaggtttta tacatcccgg aaaatatttg ttcccaggag cccatggcaa gagcacgatt
    21301 gcgagcttcg aggcgcatcg atttctgcaa ttgcggctga gccatgaggg tcatcagcaa
    21361 gggagcgaat tcatcaaagt tttttgccac gtaacccgat tttccgtgct tgacggtgga
    21421 agccggtcca ccactcgacg tgacaactgc gggaacgccg gacgcgagcg cttcaagcac
    21481 agccaaccca aacgtatccg tttcggacgg aaaagcgaat gcgtccatat ccgcgtagac
    21541 gcaggaaaga tcccggccgg tcagccaacc cttgaattcc gcgtgcttca tattttcacg
    21601 caaccaattt tcctcagcgc cctggccgat cagcacaatc cgaaaatcct tcaagccctt
    21661 tgccagtaaa tctttttcca gctgcgcaag gaaccggaca tttttttccg gagttagccg
    21721 tcccacatat ccgatttgga aagggccagc tgagcgttct cgaaacgccg gactaaacgt
    21781 ttccgtatcc acgctgtgtc ccatcaggaa gcagggtttg cccgtagctt ttccgagcag
    21841 ctcgactaat tcacgattcg gagtaaagag cagcctcgga attttgtaga accgcgcggc
    21901 cgcacggaag ctggcggatt ccgcacccgc agcaagtgga cgtgaaagat tttttggcca
    21961 cgatgataga agagatgaca tgcggcggcc agcaaattga tgaatatttg tttgccagga
    22021 cgctgcaagc gttacatgca aattatgagc cagaagcgct ccgagaatgc ccacatcgct
    22081 tggtcccgta atttgaatta cattcgggcc aaagtcctgc accacccgca atactctttg
    22141 ataatggcgc aggaacagaa gatcgaattc gtgatgccca tcaagaggaa atttgatcct
    22201 gccccgccgc aactgaattc tcgtgaggga tccgtcaacg acgatttcat cggccggtcc
    22261 ggcgtgaacc aggagaaatg gaattccgtg ctgcctggca aattccgcaa actgcttgct
    22321 ggtgttcgcg accccgtcga tttcccgata ggtacagggg aaaagcgcaa cgcggatgtt
    22381 ctcaaccgtc acgcactcac cccgcgcgga acatccagag cgcgatgcac tttcgccttt
    22441 tccctgaatg taaagtttag cgcgtggcgt accgaagtgt tttctatcat ccggaaaata
    22501 gaaaaaatat gcccaaggta agacggagga actttccata ggcaagacaa gggacgatgg
    22561 tatccaacct ggaaatccat atggaacaca cgctcatccc atcggcgcga ccccaccgga
    22621 tggtcgggat aatcgcgaat cgtatcaatt acggtctgca tgaaacggat cccgagaagt
    22681 tccgaatatt gcggcatgaa aagaatatga ctgagctgtt caactctgat ctcgttaata
    22741 aactccgaaa aagttgtggc acacgtcagg ttcagggcag cgcttggctc cagtccgtgc
    22801 cggtctcctc cggaaatgag cgggcgctgc caggcagctg caagttcaat cacactcttg
    22861 ttttccgtcc aggcgcgcat cgcattcagt tcaaaggcgt gaatgtattc cacgtagccc
    22921 tgcaggaacc tatccacgaa attccgaaaa gtcgcttccc cgaggcaata ctggtcccaa
    22981 tgcggatgat taaaaacaat caggacatcg ggaatggaat gaagcgacga caaaagttcc
    23041 accaatcgct cggccgacgg attgcgggag taagccgaaa ggtcgtccat aatcaaatgc
    23101 gcctggcttt gtggcaaatt gtgaattccc aaatgaaaaa tcgcgccctc gaatggaaca
    23161 ctccattcca cagcgaatgg aacctgaacc gggttaggcg attgacgaag gatggcgggc
    23221 gcttcaatgc tgtcatggtc cgttagcgac accaaactga ccaattcgag gttgtcctca
    23281 atctgactgc tctccagctc ataggccagc ttgggaggca agggcggagt ccagtaagcc
    23341 tgggagaaat cgacaggccg cacggatttc ttgatctgct cgttcagtgc ccagcgcagc
    23401 aagggccgct tttcagcgaa ggccggtatg aacagcaggc tttccttgga ttgattcgtg
    23461 tggctatgta aagaaaccgc tgtgcggtac tggcgagcga tttcgggctg cttccagaag
    23521 catgaaattt gtgatggagg caatgggttt cttccacgaa gatttgtttt caagttaagg
    23581 atgtcaaact acagccctgc actccagtga tctaaatatt aaatttccat actggcaata
    23641 acaatgtcta taccagcgtg taatttttct tcgtaaatcc agttgcgttg gcccatgaca
    23701 ctgctggttt gctgttgcat acgtcggctg ggcaaacagg cagcagcgtc taaattctcg
    23761 ctttacggga ccgcctactt caggagcaag aatttgcccg gtgcggctgc cacaggagga
    23821 gcgcacaccg ggcgtagtag gcatagagtg agatgccgct aactaccgtt gcggctgccc
    23881 tgaggctgca ggcggcatgg gaggtgtcag tgggggttga ggcccaagca cttttgtcag
    23941 tgcctggtat gcgcacattt cgtccgcgtt tccaccaccc actccaaccg caccgatcaa
    24001 ttgattgtcc acaacgatcg gaagaccgcc gggaacgaag tagtatgcca agccctgctc
    24061 tttgcccagg tccaggcgga tcacgcgact atccaccgtg ttgaagcgct gagcgaccaa
    24121 agcggaaggc atgcgcgcgt ataaagcggt cttcgccttc atcaatccag tctccgcacc
    24181 gatgggcagt acgccgtcca tcttgtgggc gtcaatgatt tcgcccgtcg gagaaagtac
    24241 aaaaatggcg atcgaaacat ttcctggctg tcccttttgc caatcgacac atgcatccgt
    24301 gatagcgcgt gcagtgtcag cattgatgac agccttcatc agcgttctct tggctacaac
    24361 ctccgacaca gttactttat taaatggagc gggtgttgca ccctgagccc acgcgcttgc
    24421 gcttccaaaa ccaagcgcag caatcgccag tcctagagtt gctgctgtac gaagcaaacc
    24481 tgtattcctg ctcatctttt cccccttatc ccctcgagac gttttttcct acggacgttc
    24541 catccacgct tttttacagc gcgacgaatt caatgataat ttacgtttgg ccactcccgg
    24601 caaacgggac tctacaccca gatggctcat aaaggcaaaa tttcaaaagt gaaaatttca
    24661 atgtaactcg catttaaaac gtaggccatc atgcaccgat ttcaattaaa tacataaata
    24721 atttcacaag ttgcgacttt ccatgttggc gctttgtcag ttgacacgag gtcgacgcgc
    24781 ctgatacaga cctcaaaggc attttggcgc gttacaatct tgcgctgggt gcaatttcta
    24841 atttcagaag gcggtgattg ccgtgacttt accaagcgag cgcgcgaaat attcagccat
    24901 tgtcgatcgg ccaaagctgc aactgccgaa tggcgagcgc attgtgatct ggacgatcgt
    24961 caacgtggag gtctgggaca ttgcgcgtcc catgccgcgg caggtgctcg tgccgcccac
    25021 cggggtaacg acgctccccg acgtgccgaa ctggagttgg cacgagtacg gcatgcgcgt
    25081 gggcttctgg cgatttcatg ctctctacga acgattgaaa attcgtccgt cgctttccat
    25141 caatgcgcgc gtgtgcctcg attacccgcg tgtggctcag gcctgcaaag attcaggatg
    25201 ggaattcatg ggtcatagtt tcgatcagcg acccatgcat agcgaacccg atcccgttgc
    25261 tatgatcgag cgatccgtga aaacaattca ggaatttacc gggaagtctc ctattggttg
    25321 gatggggccg ggtctaacgg aaacctatga cacacccgac cagctcaagg ctgcggggat
    25381 caaatacatt tgcgattggg catgggacga tgagccgtcc gagattgcaa cgaagcatgg
    25441 tcccttgtac acgctgccgt attcactgga gacgaatgat attcctgtga tggccgtgca
    25501 ataccatgaa gcgccctact ggacgcaaaa atgcaaagat acctttgacc gtctgtatga
    25561 ggagggcgca aaccggccga agatcatggc catcgcaatt catccatacc tgagcggcca
    25621 accttcacgc atcaaatatc tggaaaatat ttacgaatac atttcaaaat ttaccggcgt
    25681 ggtgcactgg aatggcgagc agattctcga gtggtatttg aaagcgagga aatagtggaa
    25741 cgggctccag cctgttgcct ttctgtttaa ataattaaaa aacgcaacag gctatgccag
    25801 ctttactcaa ttcacttggc ggcgggagcc atggcattga aatcagccgt gtgcgagatg
    25861 atgataatcc aacgtccgtt gcgcttggcc ataatgaagt tggagtagcc tgcgcggtaa
    25921 ggccaatccg atccatcacg cttgcgcgcc cccgtcatcg accaataatc gtccaccaca
    25981 gcaagctccg gactaatgta gtggatcgtc ttcacggtat ccgtgcgatg cgtgtttttg
    26041 tagaggtccg cgaacagccg ggcatgctca tcctcgacgg cctggcgtgt gccgagcggt
    26101 tcaccgcgcc agttcaccca atccacatta tccgccagcg atgcgcacat ggagtgggcg
    26161 tcatgcgtgt tccaaccgtt gctgaatccg gcaaccacct gcttgatagg cgcgtcttcg
    26221 ccgctgtttg cggcatcatc ggcagccgcg gcgcgcttca cgccaatcgc caacgggaga
    26281 gccagcagca ggataagaac ggctgcaaat cgtccgaagc catatcctgg ggcaatcttc
    26341 gagttcactt tcatcttgtt agctcctcat gtcatcgaaa gaattggccg cttataattg
    26401 cggagattca attagcgata tagtcctggc cacccaaccc acgggcgcct cacgttgacc
    26461 ttagggtaca ccaatctcag cgtatgcgta atacgagtca cgttatgaaa ctatgggcgc
    26521 tcggggataa accattccgc aacctttctt ttccacatcg cgtcaaaagc acatcatagg
    26581 acgtttggaa ttgccattat gaaaaagaga cttcttctcg ttggattcct tttatcctgc
    26641 cttctttcag ctgggccaat ctttgcgcac cattcctgga gcggatacga ttcgtcaaac
    26701 cttaccgcgg taaaggggac agtgacgcaa ttcgagtggg gcaacccgca catctggatc
    26761 aactttgagg tgcatgacga caagggaacg atcgaaaact ggagcgcggg cggcccgagt
    26821 ccaaaccgtt tagccaatac cggctgggat aaaaacaccc taaagtccgg ggatcagatc
    26881 gctttcgtag gcaaccgtat taaagatggt acatacagta tgaaattgca gaagatcgtg
    26941 ttgccggatg ggcgtgagtt gacctgctat agagggcgtt aaccaccgcg cttttgctgc
    27001 ataaaattac ttctttcacg caaactccgt tgtagaattc tgtttcagga aaagggtaag
    27061 gttcggagaa ccagtctgct tttacatttt cgcgagtgca atggtatagc ctgttattac
    27121 ggatacggat tttcaccgtt tgggtgtgcc cattggcaga gggcgcttga aactgccaat
    27181 cggtactttg gcgcggttac agattgcgat aggggataca aatgagaaat cggatcggcg
    27241 taatggtcct gtgcatagcg attctttccg tggtttcgat ccaggtattc gcgcatcacg
    27301 gggcatcgcc gtatgacacc tcaaaattgt ccaccttgaa gggaactgtt tcggacttcc
    27361 agttcatcaa tccccacgtg gaaatttccg tcgacgtcac caatgacaaa ggcaaagtcg
    27421 agacctggat tggcgaagcg aatagcccaa atgtcttgtc cagacatggc tgggataagg
    27481 atattattaa aaagggcgac cagattaccg tgattggaaa tcagggtaag aatggctcga
    27541 aaacgctgcg gctgcaaaaa gtgattttat cgaatggcaa ggaactcgat ccgaattcca
    27601 tcacggatta gatgcagcgc gaaagcacaa ccaatttacg ctgctggtca ttctttttaa
    27661 attcgtctga acccgcggca agtggaggaa cctcgcagtg cgcaatcgca taaacatctg
    27721 ggccttcgct ttgaacgccc ttttgttttt ttgttcggca catcccgcac aattgaatgc
    27781 gcaacaagaa cccgcgtccg cacaaccgcc cgctaccacg cccgatttgt ccggtttatg
    27841 gtcacgtctg cgcgatgcgg ccgtcccccg aggatatgga atgtatatcc tcgattacgg
    27901 taagactgat tcgccgatga ctccgtgggc agccgcgaaa tataaaacta ccagtgccat
    27961 gtatcacggt gacgatccca gcactgtgct cgctgatcct gtttttcaat gttttcctcc
    28021 cggagtgccg cgaatctatc tcttcaattt tcccgtccag atcgttcaga tccccggcca
    28081 ggtgctcatg ctctttgagt acgatcattt cgttcgccgc atttacaccg atggccgccc
    28141 gcatgattcg gagcaagggc cgctttggat gggcgattcg attggaaaat gggaaggcga
    28201 cacgctggtc gtcgatacca caaattttaa cgacaagact ttcatcgatc gcgtaggccg
    28261 tccgcacagc gatgcgcttc acgtggtgga acgcatgcgg cgcgtcgatc aaaattcatt
    28321 agaaattgat ttcacgattg acgatccaaa ggcctacaca aagccctggg gcactaagct
    28381 gatcttcgaa tcgaagccaa cctggaaagt gctggaacaa atctgcgaag acaacgccag
    28441 cttcatcgga cttaacgaaa aagccacgca gcctgcgaaa aaaaagaagt agacctctgc
    28501 tttttgtgaa taaacaattc caatcctcga tttctcattg gccctgggac tgtttgtgtc
    28561 cgtgtcttca aatcgtttgg atttctgacc ggttttttga taatcgcagg acggaccgtt
    28621 cgctgtataa tacatttgaa taattttaag acagcagact ttcaacttca gaagcttgga
    28681 aatatttggt gcgtgcatcc tcgccgaaaa tgccaaaact cctgctgcgg aaatattgag
    28741 ctatttgcga ggacttcatc gggttacatc aattgcgaat atcgaatcgc aaaaatactc
    28801 agggatcgag gatgaatcga gtgaaagcca acgggatagg aaattatttc atcatggcgg
    28861 tggcctgcgg cgcattgtcc atggcagttg ccgtatttac tccgtctccg ctttgtgcgc
    28921 aggctggagt
//
LOCUS       DASZMB010000056         3893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3079697, whole genome shotgun sequence.
ACCESSION   DASZMB010000056 DASZMB010000000
VERSION     DASZMB010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3079697"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1112
                     /locus_tag="VG272_02440"
     CDS             <1..1112
                     /locus_tag="VG272_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12567.1"
                     /translation="TGLAVDKDDNVWVYDRPDDLTSIEVEAELNPPIADCCTHPPSMI
                     HIAGHDGPGYKDGDVIGSFEAPQGHGMAVDKQGFLYLGQNNVRKYDPKDGKMVMEVPH
                     VPETPDGKPFMMPTLPTRAPGEGTAGPIAGFIIVPGAEKRARGEAAGTAAGLARKAFI
                     EKYPPTTPMIVGGIEEIRTDDAANEIYIADNAFGGRVMVFSLDKFEFKRGWGAYGHKL
                     SEISTAPEDHAYKPNGPMAKEFVGHLTFNFSNDGLVYAADRGGNRIHVTDKQGNFKQE
                     IKMAEWTGVGGSTGGVMFSNDKDQRFLYISDLTNNHIWFLDRKSGKILGTMGSMGQNA
                     GQFFGVHMEATDSKGNLYTGEVFNGERVQRFLLQK"
     gene            complement(1238..1879)
                     /locus_tag="VG272_02445"
     CDS             complement(1238..1879)
                     /locus_tag="VG272_02445"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HWF12568.1"
                     /translation="MATDSYNPKVDTYIEKAQPFAQPIMNHLREIAHKGCPDVNETVK
                     WGMPFFEHRGEILCCMAAFKKHCRFGFWGKEIRVVMRESKVAGIDKSGWFYRITRVED
                     LPADKALLDFVRQASVLIESGNQTSPMAGRNKKAKARSSAVNVKMTKEFSAALKKNKK
                     ATSVFGAFSPSAKREYIEWIAEAKRPETRDKRIATAIDWISEGKQRNWKYQNC"
     gene            complement(2022..2942)
                     /locus_tag="VG272_02450"
     CDS             complement(2022..2942)
                     /locus_tag="VG272_02450"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWF12569.1"
                     /translation="MLLVSLWPAITISLFAQATPPWSKGEGNPAADKGYEFQVPDIDN
                     IADLHGNPDAARLVLFIGGNQFFVLPELVSAFLKQHPDLQSHIFYETLPPGILLKQIQ
                     SHNTITLGNFTFAIQPDVYEADFTVVADMEKRNLVEKPMAYATNILSIMISAGNPKQI
                     HSLQDLGRTDVRLSMPNPQWEGIAKQIGDALRKAGGDSLFHSVYEAKVQDGSSYLTQI
                     HHRQTAMRIMRGESDAGVTWSSEVRFQEKIGNPIYGIEIPGEQNFTAVYGAAVLRSAP
                     HAEAARAWVAFLNSPEAQAVYKEYGFGPAK"
     gene            complement(2992..3699)
                     /locus_tag="VG272_02455"
     CDS             complement(2992..3699)
                     /locus_tag="VG272_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12570.1"
                     /translation="MTFFDRRSFFGKVAIGLATLGAAKAPADAQLVYKPADWNAADFD
                     GLVKHRARVKQVFDEIQIGGGRFLNNIKNSLNGLQFSFNIPAEQIKIVAAMHGPANML
                     NFDDFIWNKYKVGEWLKVDDPKTGQPAVRNPYLVRKSAAETPGAAQDPSDENSSFQDT
                     SIQALQARKVQFLCCHTATEEQARALIKQYSLTQQPEEIVKEMLAHTVPGVMVVAAMV
                     SCIALLQSEGHYSYVTV"
BASE COUNT          870 a         1059 c         1066 g          898 t
ORIGIN      
        1 tgacgggcct ggcggtggac aaggatgaca acgtttgggt gtacgaccgg ccggacgatt
       61 taacttcaat cgaagtggaa gcggaactca atcctccgat cgccgattgc tgcacgcatc
      121 ctccgtccat gattcacatc gccgggcatg acgggccagg atacaaagac ggcgatgtta
      181 ttggttcgtt cgaagcgccg caggggcacg gcatggcggt ggacaagcaa ggattccttt
      241 acctcgggca aaataacgtg cgcaagtacg atcccaagga cggaaaaatg gtgatggaag
      301 tgccgcacgt gccggagacg ccggacggca agccattcat gatgccgacg cttccgacgc
      361 gcgctccggg cgaaggcacc gctgggccaa tagccggatt catcatcgtt cccggcgctg
      421 aaaagcgcgc gcgcggcgaa gccgcaggca cagccgcagg actggcacgc aaggccttta
      481 ttgaaaagta cccgccgacc acgccgatga tcgttggcgg catcgaagaa attcgcacgg
      541 acgatgctgc taatgaaatc tacattgcgg acaacgcctt cggcggccgc gtgatggttt
      601 tctcgctcga caagtttgaa ttcaagcggg gctggggcgc gtacgggcac aagctttcgg
      661 aaatcagcac ggctcccgaa gaccacgcgt acaagccgaa cgggccgatg gccaaggaat
      721 ttgtcggcca cctcacgttt aatttttcaa acgacggttt ggtctacgcg gcggatcgcg
      781 gcggcaaccg cattcacgtc accgacaagc agggaaattt caaacaggaa atcaagatgg
      841 cggagtggac aggcgtcggc ggctcgacgg gcggcgtgat gttctccaat gataaagatc
      901 aaagatttct ctacatctct gacctcacga ataatcacat ctggtttctc gatcgcaagt
      961 ccggaaaaat tttaggcacg atgggcagca tggggcagaa cgcgggacaa tttttcggcg
     1021 tgcacatgga agccacggac tcaaaaggaa atctgtatac cggcgaagta tttaacggtg
     1081 agcgtgtgca gcggttcctg ttgcagaagt aatgcctaaa atttggttgc gttctatgtg
     1141 gcgcgggctt cagcctgcgg aatttcgtcc ggcaagatcg accttgaaag gacgaacctt
     1201 cgcacgctag agcccgcgcc acaacaactt tcccgcgcta gcaattctgg tatttccagt
     1261 tgcgctgctt accttcggaa atccagtcga tggcggtggc gattcgcttg tcgcgggttt
     1321 caggacgctt ggcctctgca atccattcga tatattcgcg tttcgcgctc gggctgaacg
     1381 cgccaaacac agacgtcgcc tttttgtttt tcttcagcgc ggcagaaaat tccttggtca
     1441 ttttaacgtt tacggccgag ctcctcgctt tggctttttt gtttcttccc gccatagggc
     1501 tcgtctgatt gccgctttcg atcagcacgc tggcttggcg cacgaaatcg agcaaagcct
     1561 tatccgcggg aagatcttcg acgcgcgtga tgcggtaaaa ccaacccgat ttgtctatcc
     1621 cggcgacctt cgattcgcgc atcacgacgc gaatttcctt gccccagaaa ccgaagcggc
     1681 agtgcttctt aaacgcagcc atacagcaca atatttcccc gcgatgctcg aagaaaggca
     1741 tgccccactt aaccgtttcg ttaacgtcgg gacagccttt gtgcgcgatt tcccgcaggt
     1801 ggttcatgat cggctgcgca aagggttgcg ccttttcaat gtaggtatct acttttggat
     1861 tgtagctgtc cgttgccatt gtgatcttct tccgggagaa aggttcgccg cgcgcttatt
     1921 tttccagaag attactgaat ttgtaaaggc ttgcaaaatc tattctcgag aaatggtttc
     1981 gcggcgcagg cgagacgccg gcgccacaac agccatgcaa cctacttcgc tggtccgaat
     2041 ccatactcct tgtacacagc ctgggcttcc ggggaattga gaaacgccac ccatgcgcgc
     2101 gcagcctccg cgtgcggggc actgcgcagc accgcggctc cgtagactgc ggtgaaattt
     2161 tgctccccgg gaatctcgat gccataaatc ggattgccga ttttttcctg aaaacgaact
     2221 tctgaactcc aggtgactcc ggcgtccgat tcgcctctca tgattcgcat ggccgtctgg
     2281 cgatgatgaa tctgtgtcag atagctcgag ccgtcctgaa ctttggcttc gtaaacggaa
     2341 tgaaatagcg aatcgccgcc ggccttgcga agtgcatcgc caatttgttt cgcgatccct
     2401 tcccactgcg gattgggcat agaaagccgc acgtcggtgc gaccgaggtc ctggagcgaa
     2461 tgaatttgtt tcgggttgcc cgcggaaatc atgatggaca gtatgttggt tgcatacgcc
     2521 atcggtttct ccacgaggtt gcgcttctcc atatccgcca ccaccgtaaa gtcggcctcg
     2581 taaacatcgg gctgaatagc gaaagtgaaa ttgccaagcg tgatggtgtt gtggctctga
     2641 atttgtttta gcaaaatgcc gggcggcaac gtttcgtaaa aaatgtggct ttgcaaatcc
     2701 ggatgttgtt ttaagaacgc gctgaccagc tcgggcaaga caaagaattg attgccgccg
     2761 ataaaaagga cgagcctcgc ggcatccgga ttcccatgca aatcggcgat gttgtctata
     2821 tccgggacct ggaactcgta gcccttgtcc gcggcgggat tgccttcccc tttactccag
     2881 ggcggagtcg cttgagcaaa aagcgaaatc gtgattgcgg gccacagaga aactagaagc
     2941 aagactcgcc gcgcaattcg acgagcgcat gaacgaatcg gcgcctcgca actaaaccgt
     3001 gacataggaa tagtggcctt cgctctgcag cagcgcgatg cacgaaacca tcgccgccac
     3061 aaccatgacg ccgggaaccg tatgcgccag catttctttg acaatttctt ccggttgctg
     3121 ggtgagtgaa tattgcttga tcagcgcgcg ggcttgctct tccgtggccg tatggcagca
     3181 cagaaattgc accttgcgcg cttgcagagc ctgaatgctg gtgtcctgaa aggaagagtt
     3241 ttcatcactc ggatcttgcg cggcgcccgg agtttccgct gcggacttcc ggacgaggta
     3301 tggattgcgc acggcgggct gcccggtttt tggatcgtcc accttcagcc attcgccgac
     3361 cttgtattta ttccagatga aatcgtcgaa attgagcatg ttcgccgggc catgcatcgc
     3421 cgccacaatt ttgatttgtt ccgcaggaat attaaaactg aattgcaatc cgttcagcga
     3481 gtttttaata ttattcagaa aacgtccgcc gccaatttga atttcatcga agacctgttt
     3541 tacccgcgcg cggtgtttga ccaggccatc gaagtccgcc gcattccagt ctgcaggctt
     3601 gtaaaccagt tgcgcatccg ctggagcttt tgcggcccca agcgtggcaa ggccaatcgc
     3661 aacctttcca aaaaacgatc gtcggtcgaa aaatgtcatg gaagtctcct ggattcgttc
     3721 agcaaaaacg gcgcgccgca attccttcga tcaagactag cgccggccaa actgtaaatc
     3781 gctacatttt ttccggtgat tcgatgccca gtaatccgag cgcggctacg agctgcactt
     3841 cggccaggcg gctcagttgc agtaagaaag ttttctttgc ggcgtcggat tcc
//
LOCUS       DASZMB010000057         5280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3080375, whole genome shotgun sequence.
ACCESSION   DASZMB010000057 DASZMB010000000
VERSION     DASZMB010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3080375"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VG272_02460"
     CDS             complement(<1..953)
                     /locus_tag="VG272_02460"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF12571.1"
                     /translation="MPTRREFLKRASKTTAGVFFAGCTCCKPVLGFAANAKSSREQAV
                     ANRKRQVVIGGKQTPVVDIHAHVRVPEAWDLVKNRIGRDGRPGDVQLGNPDSPANIHK
                     DVQRHLEDLDEMGIDAQALSINPFWYWADVELARKIVQIQNEKIAELCAAHPTRFVGL
                     GSVALQHPSLAAEQMEYAVKSLGMRGVAIGGSVNGDDLSVPKFHAFWAKAEELQTLVF
                     IHPQSTGAPIDEKRLKGNGFLDNVVGHPLETTLALTHLIEDGTLDLFPRLKICAAHGG
                     GYLPSYSSRGDQCLTAFSNLCKPLKKLPSEYLKQLYFDSVL"
     gene            1136..2374
                     /locus_tag="VG272_02465"
     CDS             1136..2374
                     /locus_tag="VG272_02465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HWF12572.1"
                     /translation="MSKSTSAARRKFIKYLAASPYVAALGGVAAFFERTGLAQSAQQS
                     SDVITSPAGALDVFDFEEAAHRKVMQGHWAYMASGVDDDATLRANREIFKHVQLRPRR
                     LHDATKVDMHVDFLGTTYNSPIFFCPTGGEKSFFQDGELAVARSAKAHGTLQILSTAT
                     STPVEEVCKERGQPVWYQLYATTSWDVNEKILRRVEAAGCPVVALTVDNTTGRNSETY
                     LRTRGKDLNQCLNCHETPAGPTTKERPMYDGIDMTGVRTQNPTMDWAFLDRLRKTWKE
                     KLFIKGMDTREDARLCIEHGVDGMLVSNHGGRSTETLRSTLEALPEVVAAVDGKIPVF
                     VDGGFRRGTDVFKALALGAKGVGIGRPFLWGLGAFGQPGVDRVIEIMQGELKMVMGNC
                     GTRTVADVTRDYIATPDWKI"
     gene            complement(2391..2996)
                     /locus_tag="VG272_02470"
     CDS             complement(2391..2996)
                     /locus_tag="VG272_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWF12573.1"
                     /translation="MFTLKVNSDKKVKQNNFKLNLQLPSRLQISCALVLLAYSATAFV
                     CAAQSPNLGHPLTPDEIKKIDITVVPDGRGLPAGSGSVSLGAAVYAKYCQSCHGEKGA
                     GKPQDQLTGGLGTLATGKPVKTPASYWPVATTLFDYIRRAMPITSPQSLSNDEVYAVT
                     AYILSIDGIVPGDAVLDAKSLPRVKMPNRDGFVSWWPKAPR"
     gene            complement(2983..4278)
                     /gene="soxC"
                     /locus_tag="VG272_02475"
     CDS             complement(2983..4278)
                     /gene="soxC"
                     /locus_tag="VG272_02475"
                     /EC_number="1.8.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR04555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite dehydrogenase"
                     /protein_id="HWF12574.1"
                     /translation="MREFTGKDNSKLNEEIVAGNGLINRRTLLRGALFSAGIVAAGAG
                     VASAQDNVDVDAPEWMKTPGRPFSGYGMPSKWQEKVQRIFTIAPGRAGTGVSRTPLQL
                     LEGTITPNGLHFERHHNGVPDIDPSRHQLFLHGMVKRPLVFSLEDLMRYPMESRIHFV
                     ECSGNSGIFSNPQPQQVTAGELNGLLSCSEWTGVKLSTLLADAGVNKDAKWVIAEGGD
                     AAAMSRSIPLEKCMDDAMIALYQNGEAIRPEQGFPMRLLLPGFEGNMNVKWLRRLNVL
                     DVPVDARDETSHYTELMPSGKARQFMFAMGAKSAIIKPSFGMTMKGAGLYEISGLAWS
                     GAGRVSKVEVSADGGKTWAQAALTGPVLPKALTRFRLPWKWDGQPATLLSRATDETGG
                     TQPTRASWVDQFAPGQGYHFNGIQSWGVATDGTVKNVYA"
     gene            complement(4471..>5280)
                     /locus_tag="VG272_02480"
     CDS             complement(4471..>5280)
                     /locus_tag="VG272_02480"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12575.1"
                     /translation="HCLTIVDSKGNIIETFPQYDHLFKRPHYVTINPYDPEKHIWIVD
                     DHMHAIYIFTHDMKTLVKTMGTPTVKGADGTHFNRPTFLSWLPDSTLFVADGYNGTRV
                     AKFDKDGKFLMDWGMKGTPPNDTRPYYMNNVHGVALDPQTKHVFVNDRGNHRVQVFDE
                     NGKYLYEWSMGKEPSDVHLLYIGADRNLWAYDRGTSKMLKYDLQGHFLYSWGTWGDFP
                     GGFWGVHGFSVDQDGNFYTAEVDNGRAQKFTPRAGANPNYLVSKPIYSAWK"
BASE COUNT         1195 a         1523 c         1328 g         1234 t
ORIGIN      
        1 aaaagtacgg aatcaaaata aagttgcttt agatattcgc tcggaagctt tttcaaaggc
       61 ttgcaaagat tggaaaatgc cgtcaggcat tggtcgcccc tgctcgagta ggaaggcaag
      121 tagccaccgc catgtgcggc gcagattttc aaacgcggaa agagatccag cgtgccatct
      181 tcaatcaggt gagtgagtgc aagagtcgtc tccaacgggt gaccgacaac gttgtcaagg
      241 aatccattcc ctttcaaacg cttttcgtcg attggcgcgc ctgtggattg cggatgaata
      301 aaaaccagcg tttggagttc ctccgctttg gcccaaaacg catgaaactt cggcacggac
      361 aggtcgtctc cattcacgct gccgccaatg gcaacgcccc tcatcccaag acttttcacg
      421 gcgtattcca tttgctccgc agcaagcgac ggatgctgca acgcaacgct gccaaggccg
      481 acgaaacgtg tcggatgcgc tgcgcagagt tccgcaattt tctcgttttg aatctggacg
      541 atctttcgcg ccagttcgac atccgcccaa taccagaacg ggttaatgga aagagcctgc
      601 gcgtcaattc ccatttcatc caagtcctca agatgccttt gcacgtcctt gtgtatgttg
      661 gcggggctgt ccggatttcc caattgcaca tcgcccggac gaccgtcacg gccgatgcga
      721 ttcttgacga gatcccaggc ttcgggcacg cgcacgtgcg cgtgaatgtc cacaaccggc
      781 gtctgtttgc cgccaatcac aacttgtcgt ttgcggttgg cgacggcttg ctcacgagaa
      841 gatttcgcgt tcgctgcgaa ccccaaaaca ggtttgcagc aagtgcaccc ggcgaaaaaa
      901 actccagccg ttgttttgga cgcgcgcttc aggaattccc ttcgcgttgg catgttctat
      961 tttcaattcc cgctgaattt cactttaggt tttggcgctt tctattgcgc ctacttatac
     1021 acccgaggcc gcttccaaga aaccaagtta tcgctcccgg tttgcgaagc ctccatcccg
     1081 cccacacttt ccgtctagtc agaaacaaac tgtagcgcta caatcggcaa ccatcatgag
     1141 caaatccaca tcagcagcgc gtcgtaaatt tattaaatat ctggcagcca gcccgtacgt
     1201 ggcagctctt ggcggagtcg cggctttctt cgagcgaaca ggcctcgctc agagcgcgca
     1261 gcaatcttcc gacgtcatca ccagtccagc cggcgcgtta gacgtctttg attttgagga
     1321 agccgctcac cgcaaagtta tgcaagggca ctgggcctat atggcaagcg gcgtggacga
     1381 tgacgcgacg cttcgcgcga atcgagaaat tttcaagcat gtgcaattgc ggccccgccg
     1441 tctgcatgac gctacaaaag tcgacatgca cgttgacttt ctcggaacca catacaacag
     1501 cccgattttc ttttgcccca cgggcggcga aaaatctttc tttcaagatg gcgaattggc
     1561 cgtcgcgcgt tccgccaaag cgcacggcac tttgcagatt ctttccacgg caacctcgac
     1621 accagtggaa gaagtttgca aggaacgcgg ccagccagtc tggtatcagc tttacgcaac
     1681 aacttcgtgg gatgtgaatg aaaaaatcct gcgtcgcgtc gaagccgccg gctgcccggt
     1741 tgtggccctt acggtcgata acaccaccgg ccggaacagc gaaacgtatc tccgcacgcg
     1801 aggcaaggat ttaaatcagt gtctcaactg ccacgaaact ccggcaggcc ccacgaccaa
     1861 ggaacgccca atgtatgacg gcatcgacat gaccggagtg cgcacgcaaa atccaaccat
     1921 ggactgggca ttcctggatc ggcttcgcaa aacatggaaa gaaaaacttt ttatcaaggg
     1981 aatggacacg cgtgaagacg cgcgactctg catcgaacat ggcgtcgatg gaatgctcgt
     2041 ctcgaaccat ggcggccgct ccacggaaac gttgcgctca acattagaag ctctgcctga
     2101 agttgtggct gcggtcgatg gcaaaattcc cgtgtttgtc gatggtggct ttcgacgagg
     2161 cacagacgta ttcaaagccc tcgcgctagg agccaagggt gttggtatcg ggcgtccctt
     2221 tctctggggt ttgggcgcgt tcggtcagcc gggagtcgat cgagtgatcg aaattatgca
     2281 aggcgaactg aaaatggtga tgggaaattg cggcacacga acagttgcgg acgtcacgcg
     2341 cgactacatc gccacgcccg actggaaaat ttagcgtttt aaactacaca tcaccgcggc
     2401 gctttcggcc accaactcac aaagccatct ctattcggca ttttgacgcg cggcaaagat
     2461 ttcgcgtcaa gcaccgcatc ccccggaacg attccgtcaa tcgacaagat ataagccgtc
     2521 accgcatata cttcgtcgtt gcttagtgac tgaggcgaag tgataggcat tgcccttcga
     2581 atatagtcaa acagcgtagt tgccacaggc cagtagctcg caggtgtctt tactggcttc
     2641 cccgtggcga gtgtaccgag gccgccagtc aattgatcct gcggcttccc tgcgcctttt
     2701 tctccatggc aggattgaca atacttggcg taaaccgctg cgcccaggga aaccgatcca
     2761 ctccccgcgg gtaatcctct cccgtctgga accaccgtga tatctatttt ttttatttcg
     2821 tccggcgtaa gcggatgtcc cagattagga ctttgtgcag cgcatacaaa cgctgtcgca
     2881 ctgtatgcaa gcaaaacaag cgcgcaggag atttgaagcc tcgatggaag ctgaagattt
     2941 agtttaaaat tattttgttt tactttttta tccgagttaa ctttaagcgt aaacattctt
     3001 caccgtccca tccgtcgcca cgccccaact ttgtatgccg ttgaagtgat atccctgccc
     3061 cggcgcgaat tgatctaccc aactcgcgcg agttggctgc gtgccgccgg tctcgtccgt
     3121 ggcgcgactc aagagtgtgg ccggttgccc atcccatttc catggcaagc gaaatcgtgt
     3181 aagagccttc ggcaggaccg gccccgtcaa tgcggcctgc gcccaagtct ttccgccatc
     3241 cgcggatacc tccacttttg aaactcgccc cgcgccggac cacgccaatc cggaaatttc
     3301 gtaaagtcct gcgcccttca tggtcattcc gaatgagggc ttgatgattg ctgacttcgc
     3361 gcccatcgca aacatgaact gccgcgcctt cccgctgggc atcaattccg tataatgcga
     3421 agtctcatcc cgcgcatcca ccggaacatc cagcacgttc aatctgcgaa gccatttcac
     3481 gttcatgttt ccttcaaaac cgggcagcaa cagccgcatg ggaaaacctt gctcggggcg
     3541 aatcgcttcg ccgttctgat atagagcgat catcgcgtca tccatacatt tttcgagtgg
     3601 gatgcttctg ctcatcgccg cggcatcgcc cccttcggcg atcacccact tcgcatcttt
     3661 attcacgcct gcatccgcaa gtagcgttga gagcttcact ccggtccatt ctgaacaaga
     3721 gagcagtcca ttcagttcgc ctgccgtcac ttgctgcggt tggggattgc tgaaaatccc
     3781 gctattaccg gagcattcca caaaatgaat ccgcgactcc atcgggtagc gcatcaggtc
     3841 ttcgagcgaa aataccagcg ggcgcttaac cattccgtga agaaacaatt ggtgacggct
     3901 cggatcgatg tccggcaccc cattgtggtg acgctcgaaa tgtagtccat tcggggtgat
     3961 cgttccttcc agcaactgga ggggcgtgcg cgacacgcca gtcccggcgc gtccgggtgc
     4021 gattgtaaat atacgttgca ctttttcctg ccattttgaa ggcatcccgt agccggaaaa
     4081 cggccgcccc ggggtcttca tccattccgg cgcatccaca tcaacattgt cctgcgcgct
     4141 ggcgactccc gcgcccgccg caacaatgcc cgcggaaaaa agcgcgccgc gcaacaaagt
     4201 gcgccgattg attaaaccgt tccccgcgac aatttcttca tttaatttgc tgttatcttt
     4261 tcctgtaaat tcacgcacat ttgccccctg acagtccaat catcaatcca aaaaaagccg
     4321 cctcaatgtc aaggaaagct taacgtgaca tcaaggcggc ctcaagcttc tatttgctta
     4381 ccgcgctctt tgtgtctttc aaagggtgcg gctttggatt caaatcaata aacctctcgt
     4441 gcagcgcgca accaacgccg cacgaaaaat ttacttccac gcagagtaga ttggtttgct
     4501 aaccagatag tttggattag cgccggcgcg cggagtaaat ttttgcgcgc gtccgttatc
     4561 cacttcggcc gtatagaaat tgccgtcctg atcgacgctg aatccgtgca ctccccagaa
     4621 gccgcccggg aaatctcccc aggtgcccca cgaatacagg aagtgtccct gcagatcgta
     4681 cttcagcatc ttggaagtgc cgcggtcgta ggcccacaag tttctgtctg cgccaatgta
     4741 aagcaggtga acgtccgacg gttctttgcc catgctccac tcgtacaaat atttcccgtt
     4801 ttcgtcgaac acctgaacgc ggtgattgcc gcgatcgttg acgaacacgt gtttggtttg
     4861 cggatcgagc gcaaccccgt gaacgttatt catgtagtaa ggacgcgtgt cattcggcgg
     4921 cgtgcccttc attccccagt ccatcaggaa tttgccgtcc ttatcgaact tcgcgacgcg
     4981 cgttccgttg tagccatcgg caacgaaaag agtgctgtcc ggcagccacg aaaggaacgt
     5041 cggccggttg aagtgtgtgc catccgcgcc ctttaccgtg ggcgtaccca tggtctttac
     5101 gagcgtcttc atgtcatgcg taaaaatgta aatggcatgc atgtgatcgt cgacgatcca
     5161 gatatgcttt tccggatcat acggattgat cgtcacatag tgaggccgct tgaagagatg
     5221 atcgtactgc ggaaaggttt cgatgatatt tccctttgaa tcgacaatgg tcaggcagtg
//
LOCUS       DASZMB010000058        10216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_614663, whole genome shotgun sequence.
ACCESSION   DASZMB010000058 DASZMB010000000
VERSION     DASZMB010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 10216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10216
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614663"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..420)
                     /locus_tag="VG272_02485"
     CDS             complement(34..420)
                     /locus_tag="VG272_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWF12576.1"
                     /translation="MKLFREYGTVAVIGIATMWLLSFTHARAQEPKAANAPAGNVENG
                     KRLFMKDGCYECHGREGQGSNMSGPRIAPNPPPLDVILGYVRKPTGEMPPYTAKVISD
                     SELTDIYAYLQSRPAPSKEKAAQILK"
     gene            complement(477..2423)
                     /locus_tag="VG272_02490"
     CDS             complement(477..2423)
                     /locus_tag="VG272_02490"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWF12577.1"
                     /translation="MAKSPKKSAGRRDFLKSVVAGAATLAAAPAEALPAGAQPPAELP
                     SHAMPILPPMADADPPAEPEVLTIDRSGSDYMVDVLKSLGIEYVCSVAGSSFRALHES
                     VINYGGNKSPEFISCCHEEASVAIAHGYAKIEGKPLAIFAHGTVGLQHASMAVYNAYC
                     DRVPVYIVIGNNIDATKRDAAYGEWPHSVQDAAAMVRDYTKWDDLPISLEHFGESAVR
                     AYKIAMTPPMGPVLIVADSELQEHPIEEGVNPRVPKLTIAAPPQGDSGSVAEAAKMLV
                     QAENPLIVVDRYARTQAGIDLLVELAETLQVPVIDQAARMNFPTRHPLNQSSRRKAVV
                     ANADVIMGLEATDFWSAMNTMHDQVNRTSRSIAKSDVKLISITTGDLYIKANYQDFFR
                     YTPVDLAMAADPEATLPSLIEEVKRQLTTDRKQILADRGKKLAAAHQQALELARSDAS
                     YAWDATPVSTGRLCAEIWAQVKDEDWSLVSLLRHFSWWPLRLWPFDKHYQYIGIQGGG
                     GVGYNAPASVGAALANKKHGRLSVSIQDDGDLLFGPAVLWTAAHHHIPLLSVMHNNRA
                     YHEELMHVQRMANRHNRGIDRAHIGTTFEDPAVDFAKVAQGLGVYAEGPITDPNEIAP
                     ALKRAIAVVKRGEPALVDVVTQPR"
     gene            complement(2647..3930)
                     /locus_tag="VG272_02495"
     CDS             complement(2647..3930)
                     /locus_tag="VG272_02495"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HWF12578.1"
                     /translation="MTATSLPGESLVRPRASVTDARGERAPISRWWRLVGGLSMNLAL
                     GTLYAWSVFVAPLEKEFGWSRAQTSTVFTFAAVMFAASLLIGGRLQDRFGPFWISISG
                     SILITLSFFLFSYTSSLYFLYFFYGVLGGIGNGFGYGTVVPVMAKWFPDRMGLAIGLA
                     LAGYGGGSAIFGTIANLVLFPHFGWRTSCVILSGIFFVMTMTGAFLMKNPPAVQPKES
                     KEAEDSPPQLIRNRHQFTPGEVVRTPAFYLLWLGFGLGSTAGLMVISQLIPFARNQGI
                     SSAMIATMGLVVGAFGNISGRVLSGWLSDWMGRLNTLRAVLAISSIATPMLYWVGAHL
                     AALYVLIYFVYFCYGAQASVVPSTVSDFWGTKYAGSNYGAIFTAWGFAGILGPTIGGV
                     LFDKYRNYGVAFYTATGLAIIALICIIVARRPEVR"
     gene            complement(3951..4463)
                     /locus_tag="VG272_02500"
     CDS             complement(3951..4463)
                     /locus_tag="VG272_02500"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWF12579.1"
                     /translation="MAIKEGWTGRVFEDFEVGDVYRHPLGRTVLATDNIWFTLLTQNT
                     NPIHFDHAYAAKTEFKKPLVDSTFTLALVTGQSVADISQNAMANLGWDEVRLPNPVFE
                     GDTIYSQSEVLDKRESKSRPNVGIVRFKTTGYTQDGTVVIEFKRTIMIYKRGQVPETV
                     KGPNIAPKSA"
     gene            complement(4651..5829)
                     /locus_tag="VG272_02505"
     CDS             complement(4651..5829)
                     /locus_tag="VG272_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HWF12580.1"
                     /translation="MRPLEGITVVSLEQAVAAPFATRQLADLGARVIKVERPGAGDFA
                     RGYDATVQGMSSHFVWLNRSKESLTLDLKRDEAKLVLNRLLAKADVFVQNLAPGAVDR
                     LGFSTQELRQNYPRLIVCNLSGYGSSGPYRDKKAYDLLIQAEAGLVSITGTPDTPSKV
                     GISIADISCGMYAYSGILTALLVRSKTGEGVALETSLFDSLGEWMSYAIYYTTYGGTP
                     PPRTGASHATIAPYGPARTGDDKVVYLGLQNEREWKKFCDVVLQNPQLASDQRFDSNA
                     NRVRNRPQLDQAMEKVFQKLTAAEIIARLETAKIANARMNTVQEFSEHPQLDARNRWG
                     TVESPVGTLKALFPPVTIENVEPVMNAVPALGQQTDAILGELGFDPQEIANWRKADLI
                     "
     gene            complement(5829..7004)
                     /locus_tag="VG272_02510"
     CDS             complement(5829..7004)
                     /locus_tag="VG272_02510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWF12581.1"
                     /translation="MNTKSSSTAEESHEELRSAVRELCRDYPDKYWRELDEKRAYPEA
                     FVQALTEAGYMATLIPEEYGGAGLGISEASAILEEIHHSGANAGACHAQMYIMGTLLR
                     HGSEAHKKQYLPKIADGSVRLQAFGVSEPNTGTDTTQLKTMAVRNGDHYVINGQKIWI
                     SRAEHSDLMLMIARTTPVDQVKKRTEGLSTILVDLRKAVGHGLKIRPIRTMMNHATTE
                     LFFDNLEVPVENLVGEEGQGFRYLLDSLNAERILIASECIGDARWFLERTCRYANERV
                     VFARPIGKNQGIQFPIARAYANLEAARLMVNRAATLFDSGKPCGPEANMAKLLAADVS
                     WELANVAVQTYGGFGFAEEYDIERKFRETRLYQVAPISTNLILSYLAEHVLGLPRSY"
     gene            7304..8710
                     /locus_tag="VG272_02515"
     CDS             7304..8710
                     /locus_tag="VG272_02515"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HWF12582.1"
                     /translation="MDPITKTLIHFCHGLNAKDLNNDIVERTKYMLLDYLGVAVAGSL
                     AESSAPIQKMLMRSAPKGSCTVIGTMAQTCPEYAALANGAAAHSIELDDTHQAGSIHP
                     GVVIFSTAIALSEIHPHIDFGQFVSAVVAGYEVATRLGIAVQPKFHYELGFHPTATCG
                     VFGAAVTASKLLRLSEEQMLSAVGIAGSMAAGSLEFLADGTWTKRLHPGLAAQNGILA
                     ANLAAEGFRGPGTILEGRDGFLAGYSRKPKPEFILQDLGKNFEIMRTSVKPHACCRYI
                     QAPIDGLIDLAIAHDIQPDQVERVEVAILEAGWSLVCEPSKRKYEPANAVDAQFSVPF
                     GAAVALTHRKAGLDQFSNENIQSPQIKILMNKVVLQKDARIEKNFPVEWPSFVKVYLA
                     NGQFFDRYVPSPKGDPENPLTWQELAAKFQSLATRVLPRERCDQIVNSVKDMKSSSVL
                     RNIWKLTSRSAAVATQVN"
     gene            8839..9390
                     /locus_tag="VG272_02520"
     CDS             8839..9390
                     /locus_tag="VG272_02520"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HWF12583.1"
                     /translation="MKKMWMGAVVLLALVIALPAAMHAQTSQALPIPGVDAAIDFPNE
                     HEMPDPSITYKIVFDIAKASPKIDDVNPGLTAISRYYNTLAKGGVPADHRKFVVVFHQ
                     EGTEFALTNAAFKARKDGHDNPNITLMHSMKQAGVDFRVCGQGVLAKKIDAAAIQPDV
                     QIDQWAMTTITTLQLRGYVRVPM"
     gene            9853..>10216
                     /locus_tag="VG272_02525"
     CDS             9853..>10216
                     /locus_tag="VG272_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12584.1"
                     /translation="MSTEAKCPFTGDARKHTTAVSQTNADWWPGQLNLKILHQRSPKS
                     NPMDKKFNYAQEFKSLDLNAVIKDLHTLMTDSQDWWPADFGHYGGLFIRMAWHSAGTY
                     RIGDGRGGAGNGTQRFAPL"
BASE COUNT         2311 a         2870 c         2742 g         2293 t
ORIGIN      
        1 aggctgaagc ctatgccacg taaaaacaaa aaactatttc aaaatttgcg ccgctttttc
       61 cttcgacgga gcaggccgcg attgcaaata cgcgtaaatg tcggtgagtt ccgaatcgga
      121 gatcaccttg gccgtatagg gcggcatctc tccagtgggt ttgcgcacat agcccaggat
      181 cacgtccagc ggtggaggat tgggggcaat gcggggtcca ctcatgttcg agccctgacc
      241 ttcgcggccg tgacactcat agcaaccatc cttcatgaag aggcgcttgc cgttttcaac
      301 attccccgca ggcgcattgg ccgccttcgg ctcctgcgct cgcgcgtgcg tgaagctgag
      361 cagccacatg gttgcgatgc cgattacagc gacggttccg tattcacgaa aaagtttcac
      421 gtttgctcct agtttcacct gtggcacggg ctgaaagcca gcgccactta aacccattat
      481 ctcggctgcg tcaccacatc cactagggcc ggttcgccgc gcttcaccac cgcgatggcg
      541 cgtttcaatg ccggcgcaat ttcattgggg tcggtgatgg ggccttccgc gtacacgccc
      601 aaaccttgcg cgaccttggc aaagtccact gcggggtctt cgaacgtggt tccgatgtgt
      661 gcgcgatcaa tgccgcggtt gtgacggttg gccatacgct gcacgtgcat caactcttcg
      721 tgatacgcgc gattgttgtg catcacacta agaagcggaa tgtgatggtg cgccgcggtc
      781 cacaggacgg ccgggccgaa cagcaaatcg ccatcgtcct gaatgctgac cgagaggcgg
      841 ccatgctttt tattcgcgag cgccgcgccc acggatgcag gggcgttata gcccacgccg
      901 cctccgccct gaatgccgat gtattgatag tgcttgtcaa aaggccagag gcgcagcggc
      961 caccagctga aatgccgcag cagcgaaacg agcgaccaat cttcgtcctt gacctgcgcc
     1021 catatttccg cacacagacg acccgtgctc acgggggtcg cgtcccacgc gtacgacgca
     1081 tccgatcgcg caagctccag cgcttgctgg tgtgcggcag cgagcttttt gccgcgatcc
     1141 gccagtatct gtttgcggtc ggttgtaagc tggcgtttca cttcttcgat cagcgacggc
     1201 agtgtcgcct ctggatcggc ggccatggcc agatcaactg gcgtataacg gaaaaaatcc
     1261 tggtaattcg ctttgatgta aagatcgccg gtcgtgatgc tgatcagctt cacgtcgctt
     1321 ttcgcaatcg agcgcgaagt gcggttcacc tgatcgtgca tcgtgttcat cgcgctccag
     1381 aagtccgtgg cctcaaggcc catgatcacg tcggcgttcg cgaccactgc tttgcggcgt
     1441 gaggattggt tgagcggatg gcgcgtcggg aaattcatgc gggctgcctg atcgattacc
     1501 ggaacttgca gcgtttccgc caactctact aataaatcaa tgccagcctg cgtgcgcgcg
     1561 tagcggtcta caacgatcag cggattttcc gcttgcacca gcatcttcgc cgcttcggcc
     1621 acggagcccg aatcgccttg cggaggagca gctattgtaa gttttggaac acgcgggttc
     1681 acgccttctt caattggatg ctcttggagt tcgctatcgg cgacgatcaa tacggggccc
     1741 atcggcggcg tcatcgctat tttgtaggcg cgcacggcag attcaccgaa gtgttccagc
     1801 gagatgggga gatcgtccca cttcgtgtaa tcgcggacca ttgccgcggc atcctgcacg
     1861 ctatgcggcc actctccgta agcggcgtcg cgcttcgtgg catcgatgtt attgccaatc
     1921 acgatataga cgggcacgcg atcgcagtaa gcgttgtaga cagccatcga cgcgtgttgc
     1981 aaaccaactg tgccgtgagc aaaaatcgcc aacggttttc cttcgatctt ggcatagcca
     2041 tgcgcgatgg ccaccgacgc ttcttcatgg cagcatgaaa tgaattcggg acttttgttg
     2101 ccgccataat tgatcaccga ttcgtgcagc gcgcggaaac tcgaacccgc aaccgagcag
     2161 acatattcaa tgcctagcga tttcagaaca tccaccatgt agtccgaacc agagcggtcg
     2221 atggtgagga cttctggttc agcgggggga tcggcgtcgg ccatcggagg caggatcggc
     2281 atggcgtgcg acggaagttc ggcgggtggt tgcgcaccgg caggcagagc ttcggcgggc
     2341 gccgcggcca gtgttgcggc gccggcgacc acgctcttca gaaaatctct gcggcccgcg
     2401 gatttttttg gagattttgc catgaggatt gcctccgccc gtatcactag aaattttgct
     2461 tactcattaa gaaaggcccc gcctctgaag agccgggcta cagcagattg ctgcggccac
     2521 gcgacctctg tagcccgggt cttcagaccc ggggtttttc tttgccgtct agcaatcctg
     2581 ccgctacgaa tgcgcgctga aaggcacgat acaacctgcc gaaattgatt gcaatccgcg
     2641 acacaatcac cgaacttcag gccgccttgc cacgattatg caaatcaatg caattatagc
     2701 caaacccgtg gccgtataaa atgcgacgcc gtaatttctg tatttatcga acaggacgcc
     2761 accaattgtg ggcccaagaa ttcccgcaaa tccccacgcc gtaaaaatcg caccatagtt
     2821 cgaccctgca tatttcgtgc cccaaaaatc agatacggtc gaaggcacta ctgaagcttg
     2881 cgccccgtag cagaaataaa cgaaataaat cagcacatag agcgccgcca aatgcgcgcc
     2941 aacccaatac agcattggcg tggcaatgct ggaaatcgcc agcacggcac gcagcgtatt
     3001 cagccttccc atccagtccg aaagccatcc agaaagtacg cggccggaaa tgttgccgaa
     3061 ggctccaacc accaggccca tcgtggcaat catagcgctg gagattccct gattgcgagc
     3121 gaatggaatc agctggctga tcaccataag ccctgcagtc gaaccgagtc cgaagcccag
     3181 ccacaaaaga tagaacgcag gcgtgcgtac aacttcgccg ggagtgaatt gatggcgatt
     3241 gcgaatgagt tgcggtggag aatcttcggc ttccttactc tctttcggct gaacggcagg
     3301 cggattcttc atcagaaaag cgccggtcat ggtcatcacg aaaaaaatcc cgctgaggat
     3361 gacgcaagaa gttctccagc cgaagtgcgg aaataaaaca aggttggcaa tcgttccaaa
     3421 aatggctgag ccgcctccgt atcccgcgag cgccaggccg atcgctaatc ccattctgtc
     3481 cggaaaccat ttcgccatca cggggacgac ggtcccatag ccgaacccgt tgccaatgcc
     3541 gcccagaacg ccatagaaaa aatagaggaa gtacaaactg gatgtgtacg agaagagaaa
     3601 aaaactcagc gtgattaaaa tgcttccaga aatggaaatc cagaaagggc cgaagcggtc
     3661 ctgcaatcgc ccgccgatta atagcgacgc ggcgaacatg actgcggcaa acgtaaacac
     3721 ggtggaagtc tgcgcgcggc tccagccgaa ttccttttct aacggcgcaa caaaaacgct
     3781 ccaggcatat agcgtgccga gagccaaatt catggaaagg ccgcccacca ggcgccacca
     3841 gcggctgatc ggcgcgcgct caccgcgcgc atcggttacg gaagcgcgcg gacgcaccaa
     3901 tgattcgccg ggaagggaag ttgccgtcat gttctatctt aaaataaaga ttatgcgctc
     3961 ttcggtgcga tgttcggacc ttttacggtc tcggggactt gtcctcgttt gtagatcata
     4021 atcgtgcgct tgaattcgat cactacagtt ccgtcctgcg tgtagccagt ggtcttgaat
     4081 ctgacaatgc ccacgttggg cctcgatttc gattctcgct tgtcgaggac ttcactctgc
     4141 gaataaatcg tgtcgccctc gaagacggga ttcggcaggc gcacttcgtc ccaaccaaga
     4201 ttcgccatcg cattttgaga aatgtcggcc accgattgcc cggtgacaag cgcgagagtg
     4261 aaggtggaat cgaccaatgg ttttttgaat tctgttttcg ccgcgtaggc gtggtcaaag
     4321 tggatggggt tcgtattctg cgtcagcaaa gtgaaccaga tattgtccgt tgccagaact
     4381 gtgcggccga gcggatgccg gtatacatcg cccacctcaa aatcttcaaa cacgcgcccg
     4441 gtccagcctt ctttgatcgc catgatgaca ctcctttgta attaatcccc ggaacatttt
     4501 tttctgtcca actgtcattc cgagcgaagc gaggaatctc tctttctctt cgcgaatcta
     4561 aatcgaagag agattcctcg cttcgctcgg aatgacagac ggaactagat tttgttcgca
     4621 cactgtttga tacaaatatt gccgcacaat ttaaattagg tccgctttgc gccagttcgc
     4681 gatttcttgc ggatcaaaac cgagttcccc caaaatggcg tctgtttgct gaccgagcgc
     4741 gggaaccgcg ttcatcaccg gttccacatt ttcaatcgtc accggcggaa aaagcgcctt
     4801 cagcgtgcct acaggcgact caaccgtgcc ccagcggttt cgcgcatcga gttgtggatg
     4861 ttcggaaaat tcctgcaccg tgttcattct tgcattggcg atcttcgccg tttcaagccg
     4921 cgcaatgatt tccgcggccg tcagcttctg aaaaactttc tccatcgcct gatcgagttg
     4981 cgggcgatta cggacacggt tggcgttgga atcgaagcgc tggtcggaag cgagctgcgg
     5041 attctgcagc accacgtcgc agaatttttt ccattcgcgc tcattctgca gacccagata
     5101 cacaacttta tcgtcgcccg tgcgagctgg cccatacgga gcgatggtcg catgactcgc
     5161 gcccgtgcgc ggcggaggtg tcccaccata cgtcgtgtaa tatatggcgt agctcatcca
     5221 ctcgccgagc gaatcgaaaa gggaagtctc cagcgcgacg ccttctccgg ttttcgatct
     5281 gaccagcaga gcagtaagaa ttccggaata ggcatacatg ccgcaggaaa tatcggcgat
     5341 cgaaatgcct actttggatg gcgtgtcggg tgtgccggta atggagacca aaccggcttc
     5401 agcctgaatt aataaatcat aggctttctt gtctctgtag gggccggaag acccgtagcc
     5461 ggaaagattg cagacaatga ggcgcggata attctgccgc aattcctgcg tgctgaaccc
     5521 gaggcggtcg acagctcccg gcgccagatt ctgcacaaac acgtcggcct tcgcgagcag
     5581 gcgattcaga accagtttcg cttcatcgcg ttttaaatcc agcgtgagag attccttgga
     5641 gcggttcagc caaacaaagt ggctcgacat tccttgtacg gttgcgtcgt agccgcgcgc
     5701 aaaatctcca gcgccggggc gttccacctt gatgacgcgc gcgcccagat ccgccaattg
     5761 gcgagtggca aacggcgcag ccaccgcctg ctcgagggat acgaccgtga ttccttcgag
     5821 aggccgcatt agtaggacct ggggagtccc aacacgtgct ccgccagata gctcagaatt
     5881 aaatttgtgg agatgggcgc cacttgatag aggcgcgttt cacggaattt tctttcaata
     5941 tcgtactctt ccgcaaaacc gaatccgcca taggtttgca ccgcgacgtt cgccagttcc
     6001 cacgaaacat ccgccgcgag cagctttgcc atgttggcct ccgggccgca cggtttgccg
     6061 ctatcgaaga gtgtggctgc gcgattgacc atcaaccgcg ccgcttccag atttgcatac
     6121 gcgcgcgcaa tggggaattg gataccctga ttttttccga tcggcctggc gaaaacgact
     6181 cgctcatttg catagcggca agtgcgttcc agaaaccagc gcgcgtcgcc gatacattcc
     6241 gacgcgatca ggattcgctc cgcattcaag ctgtccagga ggtagcggaa tccctggcct
     6301 tcttcgccca ccagattctc gacgggcact tcgagattgt cgaaaaataa ttcggtggtg
     6361 gcgtggttca tcatcgtgcg gatcggacga atttttaatc cgtgtcccac cgctttgcgt
     6421 aaatcgacca aaatggtcga aagcccctcg gtgcgctttt tcacctgatc cacgggagtg
     6481 gtgcgcgcaa tcatcagcat caggtcagaa tgttcggcgc gcgaaatcca gattttctgt
     6541 ccgttgatca cgtagtggtc gccgtttcgc accgccatcg ttttcagctg cgtcgtgtcg
     6601 gtgccggtat tcggttcgct cacgccaaat gcttgcaggc gcacggagcc atctgcaatc
     6661 ttcggcaaat actgtttctt gtgcgcctcg gaaccgtgcc gcaaaagcgt gcccatgatg
     6721 tacatctgcg cgtggcatgc cccggcattt gcgcccgagt gatgaatttc ttccaaaatt
     6781 gccgatgctt ccgaaatgcc caggccggcc ccgccatatt cttccggaat cagcgtggcc
     6841 atgtaaccgg cttccgtcag agcctgcaca aaagcttccg gataggcgcg cttctcgtcc
     6901 agttcgcgcc agtatttatc aggataatct cggcacaatt cgcggactgc tgatcgtaat
     6961 tcttcgtgcg attcttcagc agtgctgctc gatttcgtgt tcaatgtccg gccccatttt
     7021 ctcgcgggtg actataaaaa actcttccgt agagaatact caatcgccca ggatttttaa
     7081 atggcagaag gcacgatacc aaaataattt tgtggggtat cttatatggt agcgggaaga
     7141 atcaactttg tgatgtatgg cgcaaatttc tcctatcatg gaaaattggg tgaacggttt
     7201 tgtagaggcc gttccatctc acgcttttgg gttggagcgc gggcacggcc gcggcaaatg
     7261 aaacctgact gaaaattgcg gcaaccggca gattggtgga cccatggatc ccataactaa
     7321 aaccctcatt catttttgtc acgggctgaa cgccaaagat ttgaataatg acatagtgga
     7381 gcgcacgaag tacatgcttc tggattatct cggcgttgcc gtcgccggtt cgcttgcgga
     7441 atcctctgcg cccattcaaa aaatgttgat gcgcagcgcc cccaaaggct cgtgcacggt
     7501 gatcggcacg atggcgcaaa cctgcccgga atatgccgca ctggcaaatg gcgccgccgc
     7561 tcacagcatt gagctggatg acacgcatca ggccggttcc attcatcccg gagtggtcat
     7621 tttttccacg gccatcgcac tcagcgaaat tcatcctcac atcgattttg gccaatttgt
     7681 ttccgctgtt gtcgccggat atgaagtcgc aacacgcctc ggaattgcgg ttcaaccgaa
     7741 atttcactat gagcttggtt ttcatcccac cgcgacatgc ggcgtatttg gcgcagccgt
     7801 aaccgcttcc aaacttttgc gcttgagcga agagcaaatg ctgtcagccg taggcatcgc
     7861 gggaagcatg gccgccggct cgctcgaatt tctggccgat ggcacatgga cgaagcgttt
     7921 gcatcccggc ctcgcggcgc agaacggaat cctcgccgcg aatcttgccg ccgaaggttt
     7981 tcgtggtccc ggcacaatcc tcgaggggcg tgacggtttt ctggcgggat attcgcgcaa
     8041 gccgaagccg gaatttatcc tccaggatct cggaaaaaat tttgaaatta tgcgcaccag
     8101 cgtcaagccg catgcctgct gccgctacat tcaagcgcca atcgacggct tgattgatct
     8161 cgcaatcgct cacgacatcc agccggatca agtggagcgc gtcgaagtag ctatacttga
     8221 agcgggatgg tcgctggtct gcgagccttc caaacgaaaa tatgagccgg ccaacgcggt
     8281 ggacgcgcaa ttttccgttc ccttcggagc tgcggtcgct ctgacacatc gaaaggcggg
     8341 actcgatcaa ttttcaaacg agaatatcca gtcgccgcaa attaaaatcc tgatgaacaa
     8401 agtagttttg caaaaagacg ctcgcatcga aaagaatttt ccagtggaat ggccgtcctt
     8461 cgtaaaagtt tatctcgcca acggtcaatt ttttgaccgg tacgttccgt ctccaaaagg
     8521 cgatccggaa aatcctttga cgtggcagga attggccgcc aagtttcaat cgctcgcgac
     8581 acgcgttctg cccagggagc gttgcgatca gatcgtgaat tctgtgaagg acatgaaatc
     8641 gtcctccgtc ctgcggaata tctggaaatt gacttcccgt tcggcagccg tcgccacgca
     8701 agtgaattag ttccgtggcg caggctgagc ccgcgccacc gcaccgcgaa aaagtgttca
     8761 cagaggctgt tttaaggggt gtagaatacc gcccatagcg ggcagtctaa agtaacgatc
     8821 aagttcacgg agagcaaaat gaaaaaaatg tggatgggtg ctgtcgtact actcgcactt
     8881 gtaattgcgc ttccggccgc gatgcacgcg caaacttcac aagcgctgcc catacccggc
     8941 gtggatgccg ccatcgattt tccgaatgag cacgagatgc ccgatccgag catcacctat
     9001 aaaatcgtgt tcgatatcgc caaggcctct ccgaaaattg acgatgtgaa ccctggccta
     9061 actgcaattt cgcgttatta caacacgctg gccaaaggcg gcgtgccggc tgatcaccgg
     9121 aagttcgtcg tagtcttcca tcaggaaggc actgaatttg cgctcaccaa tgcagccttc
     9181 aaagcacgca aggatggcca cgacaatccg aacattaccc tgatgcacag catgaagcag
     9241 gctggcgtgg atttccgcgt ttgcggccag ggcgtgctcg ccaagaagat cgacgccgcc
     9301 gccatccagc ctgacgttca gatcgatcaa tgggcaatga ccacgatcac gactctgcaa
     9361 ttgcgcggat atgttcgcgt gccaatgtaa gaatttgtcg agcaagggaa aagttcgctc
     9421 ccggtatctc ttcccggccc gcgaagttgg tcctacatcg cgcctccggg gcttcgttgt
     9481 gttccccgca tcgaattcgt taaatggaag gccggactta tgacgtctgg cctttttctt
     9541 ttgtcatttc gtcaatctag agagcgttgg acagccgttg gctgtgatgt agactctctc
     9601 actcgccgcg gtacaaaaac tcaggctggt catgcggccc aatcaagatt cgcaatccac
     9661 taccgtgata taaatttttt cactcgacgt cgtgcaagaa ctcgatcact tgagatacaa
     9721 gcgtgggtgc gataccaagg caggacgaac tggacagcga gaatgcggtt ccttgatttg
     9781 actgatctgt acgcaaactt taagctttaa acttatacga gtaacgattg aaaacaacga
     9841 tgggagaaaa gtatgtcaac tgaagcaaag tgcccgttca cgggcgacgc tcgcaaacac
     9901 accacggctg tgtcccagac gaatgcagat tggtggccgg gacaattgaa cttgaagatc
     9961 ttgcaccagc gctccccgaa gtccaatccc atggacaaga agttcaacta tgctcaggag
    10021 ttcaagagtc ttgacctgaa tgctgtcatc aaggacctcc atacgctgat gacggattcg
    10081 caggactggt ggccggcgga cttcggccac tacggagggt tgttcattcg catggcctgg
    10141 cacagcgcag gcacgtaccg cattggcgat ggccggggcg gtgcagggaa tggcacacag
    10201 cgctttgctc ccctga
//
LOCUS       DASZMB010000059         3662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2540907, whole genome shotgun sequence.
ACCESSION   DASZMB010000059 DASZMB010000000
VERSION     DASZMB010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2540907"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..888)
                     /locus_tag="VG272_02530"
     CDS             complement(58..888)
                     /locus_tag="VG272_02530"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWF12585.1"
                     /translation="MGVWIMAGMTFREAARKKILWTALLAGLGFLLVFGIGLRFQIGD
                     FRNSGVPPFLRYQVISAMLMIGLYTVDLLAVVMTTLTSVDTISGEITSGTIHAIATKP
                     MARWQILIGKWLGFVAMSGVYVALMFGGTIAEGYWIGGVMARNPVHGGLLVFLECVVA
                     LSVTFMFGTWFSTLTSGVITLGMLGVAFLGGWLEQMSGFTEGSRLAMVGIISSLLMPS
                     EAVWRRAAFEMESPLSASLQFSIFSDISIPSAAMIGYACVYLFIALGIAVYHFGERDL
                     "
     gene            complement(888..1775)
                     /locus_tag="VG272_02535"
     CDS             complement(888..1775)
                     /locus_tag="VG272_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF12586.1"
                     /translation="MRKVFGDKVAVRDLTLTVRKGEIFGFLGPNGAGKSTSIKMLLGL
                     ATPTSGEAYVLGVPAGGVEVRRKIGFLPENFRFYEWLTPAELLHLHGRLCGVSNSTLS
                     KRVPSLIDLVGLTPHINKRLQNFSKGMLQRAGLAQALIHEPDIIFLDEPTSGLDPLGR
                     RMVRDIIRAQRDRGATVFLNSHILSEIEITCDEVVFIREGEVVTSRDLRKQHEEAARV
                     EVRGKKLTAECVAGLSRWGDSVEVDEERLTMTVSSRDALPDILRFLVGSGAEVYQFTP
                     QRLSLEELFINVMGEDRGF"
     gene            complement(2159..3091)
                     /locus_tag="VG272_02540"
     CDS             complement(2159..3091)
                     /locus_tag="VG272_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12587.1"
                     /translation="MTTAASSNKRIWLVLIAVVIIVVGASVPAVRNAALKSLQSLRMQ
                     KVQAVNADFSAYVDPNSNPTLHQMVTQMISDKVQVETNEEDRTVSDLASAKQMAGFDV
                     QLLSARKDAPEIVVGGQHKIDVVVDRARLQAIATEAGHSDIVLPQSIDGTTVAVQVPR
                     SVQLQYGTCPGPTSAGQVVANNLTGPTPTTTQFSDCVRLREGPDPVVNVPSGLNVGGL
                     AEIGLETAGMTPSQASDFLHTIDWKATLTLAVPRALRSYQVVKVDGTNGTLLSMAGRR
                     GPGYALIWAKNGIVYSLTGFGDSSQAVTLADSLK"
     gene            complement(3259..>3662)
                     /locus_tag="VG272_02545"
     CDS             complement(3259..>3662)
                     /locus_tag="VG272_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12588.1"
                     /translation="AQDMVRVRWHAGVHNVIRGVTKNMFAAVHYNPFFAIAAILLSVL
                     MSVSPFLGVAFASGWARIFAGIAAATVVIIHAAMIWSTDASPLYGFTHPLGALLFCWM
                     ISRSAIITSMRGGVVWRDTFYPLDDLRKGMV"
BASE COUNT          889 a         1013 c          975 g          785 t
ORIGIN      
        1 atatttcaaa aaagggggga attcgtcaaa agagatatta ttttgttgag attctggtta
       61 gaggtcgcgc tcgccgaaat ggtagacggc gatgcctagc gcgatgaaca gatacacaca
      121 cgcgtaaccg atcatcgcgg cgctgggaat ggaaatatcc gagaagatag aaaattgcag
      181 cgatgcggaa agcggcgatt ccatctcaaa tgctgcgcga cgccacacgg cttcgctggg
      241 catgagcaag ctggaaataa ttccgaccat cgccagccgc gaaccttccg tgaagccgct
      301 catctgctca agccatccgc cgagaaacgc gacgccgagc atgcccagag tgatcacgcc
      361 gctcgtgagc gtcgaaaacc acgtgccaaa cataaatgta acggaaagcg cgaccacgca
      421 ctccaggaaa acgagcaggc ccccgtgcac cggattgcgc gccatcacgc cgccaatcca
      481 atagccttcg gcaatcgtcc ctccgaacat cagtgcgacg tacacgccgg acatcgccac
      541 gaaccccagc catttaccaa ttaaaatttg ccagcgcgcc atgggcttgg tagcgatcgc
      601 gtgaatcgtt cccgaagtaa tttctccgga aatcgtgtcg acggacgtga gcgtggtcat
      661 cacaacggca agaagatcga cggtgtaaag gccgatcatg agcatcgcgc tgatcacttg
      721 gtagcgcaaa aacggcggca caccggaatt gcgaaaatcg ccaatctgaa aacgcaatcc
      781 aatcccgaac accagaagaa atcccagtcc ggcgagtaga gcggtccaca aaattttctt
      841 gcgcgccgct tcgcgaaacg tcatgcccgc catgatccac acgcccatta gaatcctcga
      901 tcctcgccca tcacgttgat aaacaattct tccagtgaca gtcgctgcgg tgtgaactgg
      961 taaacctcgg cgccggatcc gacgagaaac ctcagaatgt ccgggagagc atcgcgagac
     1021 gaaacagtca tcgtgaggcg ttcctcgtcg acttccacgg aatcgcccca gcgcgaaaga
     1081 ccagcgacgc actcagcggt caattttttt ccgcgcactt cgacgcgcgc cgcttcctcg
     1141 tgctgctttc gcaggtcgcg gctggtcacc acctcgcctt cgcggatgaa aacaacttca
     1201 tcgcaggtaa tttcaatttc actgaggatg tgcgaattca aaaataccgt ggccccgcgg
     1261 tcacgctgcg cacgaatgat gtcgcgcacc attctgcgtc cgaggggatc gagtcccgag
     1321 gtcggctcat ccagaaaaat aatgtcaggc tcatgaatca atgcctgcgc aagacctgcg
     1381 cgttgcagca tgcctttgga aaaattctgt aaccgcttgt tgatgtgcgg agtgagcccg
     1441 accagatcga tgagactcgg aacgcgtttg gaaagcgtag aatttgaaac gccgcagagt
     1501 cgtccatgca gatgcaaaag ttcggcgggc gtcagccatt cgtagaagcg aaaattttcc
     1561 ggaaggaaac cgatctttcg gcggacttcc acgcctcccg cgggcacgcc cagcacgtag
     1621 gcttcgccgc tcgtgggtgt ggcgagtccc aagagcattt tgatcgaagt gcttttgccc
     1681 gctccgttgg ggccgaggaa accaaaaatt tcgcccttgc gcacggttaa ggtcaagtcg
     1741 cggaccgcga ctttgtcgcc aaaaactttg cgcaaatttt ttgtttgaat ggcgaaatgt
     1801 tcgagttcgg gcagcttcgt ctcctgttca gctcaggcta tagagtctgt gtgagaactg
     1861 taaactcgtt gtgggtcgtg gctttagcca cgacataagt gctgcaaaat aagttcggct
     1921 ttagccgctg aagttccaac attgcagttc tcacacagac tctataactt attttttcga
     1981 tttcaatttg ggtttgcagt tcaagaggtc cgggcttcaa ccccgacgtt cggattcgca
     2041 ttcatgcggc ttcggccact gaagcttcag ggcctaaagg ccgtctcggt gcgaacttta
     2101 ttgtccggcc tgaaggtcgg acctcctgac agcaaaatcc acgaattaaa gcgatatttt
     2161 atttcagaga atcggccaaa gttacggctt ggctcgaatc gccgaatccg gtgagcgagt
     2221 aaacgattcc attcttcgcc caaatgagtg catagcccgg gccacgccgc ccagccatgc
     2281 tgagcagggt accgttcgtc ccgtccactt tcactacttg ataggaacgc agagcgcgcg
     2341 gaaccgcaag cgtcagcgtt gccttccaat cgatcgtgtg gagaaaatcg ctggcttggc
     2401 tcggtgtcat tcctgcagtc tcgaggccaa tttccgcaag cccgccgaca ttgagacctg
     2461 acggaacgtt tacgacaggg tctgggccct cacgcagacg gacgcaatcg ctgaactgcg
     2521 tggttgtcgg cgtcggcccg gtgaggttat ttgcgaccac ctgtccggcg ctcgtagggc
     2581 cggggcacgt cccgtattgc aattgcacgg aacgcggaac ttgcacggcg acagttgttc
     2641 cgtcgattga ctgcggcaga acgatgtccg agtggcccgc ctcggtggca atcgcctgca
     2701 ggcgtgcgcg gtcgacgacc acatcgattt tgtgctgtcc gccgaccaca atttccggcg
     2761 catcttttcg tgcgctgagc aattgcacat cgaagcccgc catttgtttc gcgctcgcga
     2821 ggtcgcttac tgtgcgatct tcttcgttgg tttcgacttg gaccttatcg gaaatcattt
     2881 gcgtaaccat ctggtgcagc gtggggttcg agttcgggtc aacgtaggca gaaaaatccg
     2941 cgttgactgc ttgaactttt tgcatgcgta gtgattggag agacttcagc gctgcgtttc
     3001 taactgccgg aacgctggcg cctaccacga taatcactac agcaatgaga accagccaaa
     3061 tgcgtttgtt cgaagaagcg gctgtcgtca ttggatcctc tttctaataa acggagttgc
     3121 agaaaattag tgtatccacg ctttcaagtt agcaaacaaa tctgaaattt acatgaaggg
     3181 tttaagcggc gcgggcttcg gcctgcgggg tttagtcatt gcaaggacta agccccacat
     3241 gttgaagcct atgccacttt aaaccatccc ttttcgcagg tcatccagtg gataaaaggt
     3301 gtcacgccag acaactccgc cgcgcatcga agtgatgatg gcggagcggc taatcatcca
     3361 acaaaagagc agcgctccca agggatgcgt aaagccgtag agcggtgaag catctgtgga
     3421 ccaaatcatt gccgcatgaa ttatgacgac ggtagcggct gcaattccag caaagatgcg
     3481 cgcccaaccg gacgcaaacg caaccccaag aaacggggag acactcataa gtacggaaag
     3541 caaaatcgca gctatggcaa aaaacggatt gtaatggacc gcggcaaaca tattttttgt
     3601 gacgccgcga atcacgttgt gtacaccagc gtgccatcga acacgaacca tgtcctgcgc
     3661 ca
//
LOCUS       DASZMB010000060         6029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4730522, whole genome shotgun sequence.
ACCESSION   DASZMB010000060 DASZMB010000000
VERSION     DASZMB010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4730522"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1452)
                     /locus_tag="VG272_02550"
     CDS             complement(<1..1452)
                     /locus_tag="VG272_02550"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HWF12589.1"
                     /translation="MKLASRFAGVLILSICGAAFARAATVTGSVKSPGGTPFRGAFVQ
                     AQNTGTRVLVSVLTDKDGHYRIENLPAGKYQVQVKAVGYKTDPQKDVSLSVEQNASYD
                     FSLARGMVRWSDLSQYQGEVLFPEAKGKDLLVGRCFACHGFQSRMASVRRDEDGWRDR
                     VAYMRDSMHFFLGAVGGSFDDKDAADTASYINSLFGEESVLPKSPAEMPKYKELVRTF
                     DDDAMKIVYVEYELPGPSRMPWSAFPDKDGNYWMPYYGSANKIGRLDPKTGEVQEYSV
                     PNHGTAAIHSAVPAPDGSVWLTEQGSNKLGHWNPATRTITEYQDSYSPGKEGTVAGGS
                     KHTLRIDAQGRIWATGGPLTVFDPKTQKFTQIAEIPSAYGLDLDKDGNCWFAEYVPDG
                     KIGKVDAKTLKVTKWQPPTADARPRRIQVASDGTVWFAEFQAGKIGHFDPKTETFKEY
                     NLPGPETTPYALEIGKNGTVWYSSEHLDIIGSLD"
     gene            complement(1551..3005)
                     /locus_tag="VG272_02555"
     CDS             complement(1551..3005)
                     /locus_tag="VG272_02555"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HWF12590.1"
                     /translation="MQPHARLKAGATEPDFPINSEDGRNPVTGSQRGEAVAAKSKNAK
                     SPRIKSRAKSVPEKSPVVRRKKLKVAIAGFGTVGRSVAKILGQEASETFELTHIFNRD
                     VARKKVDWVPASVRWTESINEVLNSDVDIFVELAGGIKPAGDWIRKALRAGKSVVTAN
                     KQLIAESGPELVELAREEGRRLEFGASVAGGIPAIMGIQDGLAGDRLFKIAGVLNGTC
                     NFILTKMEATGASFESALREAQELGYAEADPTADVEGYDARAKLVILAQAGLRVRVQS
                     EQIPCWPISAVEAVDFGYARELGCTIRQISLAKKETEDGLSVRAFVRPALVPASSLVA
                     HVEGSKNLVMATGQYAGQMVFSGFGAGGDPTAVAVISDLWSIARSGTAAPGTVHEVET
                     PKDVSGEFTAPYYLRFVVKDRPGIIAAVATVLSRYGIGIDAVLQRPGYPHSALPFIIT
                     LETARSAQLGCALQEINRLDFHVEPVLCMPILLD"
     gene            3189..4064
                     /locus_tag="VG272_02560"
     CDS             3189..4064
                     /locus_tag="VG272_02560"
                     /inference="COORDINATES: protein motif:HMM:NF024763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWF12591.1"
                     /translation="MAQIDQRLPQNVEGEFYVDESCIDCDACRQIAPASFRDHGGKSS
                     VFHQPENPGELHRAFMALVACPTASIGTVSNLNARSGVAAFPVHVVDNVYFCGFTAES
                     SFGAWAYLIVRPPEQGGNVLVDSPRFNGPLVKRMEEMGGVSLMLLSHRDDIADHAKFA
                     AHFKCPRILHEGEGSATRGIERIITGTASVQIDDDLLLIPTPGHTRGHQVFLYHNKVL
                     FTGDHLSWSPARNSLKAFRDVCWYSWTEQTHSMEKLLAYNFEWVLPGHGRIGHTTPEE
                     MHTKLEDCVARMKKQ"
     gene            complement(4292..5497)
                     /gene="pcaF"
                     /locus_tag="VG272_02565"
     CDS             complement(4292..5497)
                     /gene="pcaF"
                     /locus_tag="VG272_02565"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: protein motif:HMM:NF006551.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HWF12592.1"
                     /translation="MTSAYICEGFRTPIGRYGGVLSSVRADDLAAVPLKALYSQFQKS
                     DWESVDEVIFGCANQSGEDNRNVARMALLLSHFPHTIPAMTVNRLCGSGMEAVTVAAR
                     AITADEISLAVAGGVESMSRAPFVMPKATAPFSRNAEIYDTTLGWRFPNPQTIKMYGN
                     ASMPETAENVAAEYKVERAAQDCFALRSQQKAAAAQKAGILGEEIVPVPIPGPKGTIR
                     PVHEDEHIRPDTTIEALSKLPTPFREGGTVTAGNASGINDGACAMFVASEDAAANFGL
                     KPIARVVGGAAAGVAPDFMGIGPIYAVRKLLARWKISMSNVDVFELNEAFAAQALAVT
                     RELGLPDDSEKVNPNGGAIALGHPLGMSGARLVLTAARQLQRIKGRYALASMCIGVGQ
                     GIAILLERV"
     gene            complement(5659..>6029)
                     /locus_tag="VG272_02570"
     CDS             complement(5659..>6029)
                     /locus_tag="VG272_02570"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWF12593.1"
                     /translation="LPDGLQYKVLTAGTGKKAVESDTVLCNYKGTFIDGQEFDSSTQA
                     GKPVPFEVKNVIPGFREVLQLMPAGSKWQVVIPSQLAYGERGAGGVIGPNSTLVFEIE
                     VVSIQATPGSDVPSAPTPAK"
BASE COUNT         1290 a         1886 c         1492 g         1361 t
ORIGIN      
        1 atcgaggctc ccaattatgt ccaggtgctc ggacgagtac cacaccgtgc cattctttcc
       61 gatctccaac gcgtacggtg tcgtttccgg tcccggcaaa ttgtattcct tgaatgtttc
      121 cgtcttggga tcgaaatgtc cgatctttcc tgcctgaaat tcggcgaacc atacagtacc
      181 gtcgctggca acttgaatgc ggcgagggcg cgcgtccgcg gttggcggtt gccatttcgt
      241 taccttcaaa gttttggcgt ccactttgcc gatttttcca tcgggcacat attccgcgaa
      301 ccagcaattt ccgtccttgt cgaggtcaag tccgtacgcc gagggaattt cggcgatctg
      361 cgtaaacttt tgcgtcttcg gatcgaacac tgtcagcggg ccgcccgttg cccagattct
      421 tccctgcgca tcgatccgca gcgtgtgctt ggacccgccc gcaactgtgc cttctttacc
      481 tggtgaatac gagtcctgat attccgtgat cgtccgcgtt gccggattcc aatgtcccag
      541 cttgttcgat ccctgctccg tcagccagac gctaccatcc ggcgcgggca cagcagaatg
      601 aatcgctgcc gtaccatgat ttggcacgga atactcctgc acttctcccg ttttcggatc
      661 gagcctgcca attttatttg cgctgccgta gtacggcatc cagtagttgc catctttatc
      721 cgggaatgca ctccacggca tgcggctcgg gccaggcaat tcgtactcga cgtatacaat
      781 cttcatcgca tcgtcatcga aagttcgcac cagctcttta tatttcggca tctcggccgg
      841 agactttggc aatacggatt cctcgccgaa aagcgaattg atgtaagaag ccgtgtccgc
      901 cgcatccttg tcatcaaacg acccgcccac cgcgcccagg aaaaaatgca tggaatccct
      961 catgtaggca acgcggtcgc gccagccatc ttcatcgcga cgcaccgaag ccatgcgcga
     1021 ttggaatcca tggcacgcga aacaacggcc aacgagcaaa tctttgccct tcgcttcggg
     1081 aaataaaact tcgccttggt attgagaaag atcgctccag cggaccattc ctcgcgccag
     1141 cgaaaaatcg taggaggcgt tctgctccac ggacaaactt acatcttttt gcggatcagt
     1201 cttgtagccc acagccttca cttgtacttg atattttccg gcgggcaaat tctcgatgcg
     1261 gtagtggcca tctttatccg tcaaaacgct gaccagaact cgcgtgcccg tattttgcgc
     1321 ctgcacgaac gcgccacgga agggagttcc gcccggactt ttcaccgagc cagtcacggt
     1381 cgccgctctg gcaaatgcgg ctccgcaaat ggacaggatc agaacgcccg cgaacctgga
     1441 agctaacttc atggctcccc cctttttcac gtctcggaaa tcgaacgatg cggcggatgg
     1501 tctgaacgtg cgcactccgc cttcggtatg tgccggattc taccgcaaaa ctaatccagc
     1561 aggataggca tgcaaagcac gggctccacg tggaagtcga gccggttaat ttcctgcaac
     1621 gcgcaaccca gctgtgcgct gcgagcagtt tcgagagtga taataaatgg cagcgcggaa
     1681 tgcggatatc caggccgctg cagcacagcg tcaatgccga tcccgtagcg cgataagacc
     1741 gttgcaacag ccgcgataat cccgggtcgg tcctttacga cgaagcgcaa ataatagggc
     1801 gcggtaaatt cgccgctcac atccttcggt gtctctactt catggaccgt tccaggagcg
     1861 gccgtaccgc tccgtgcgat ggaccacaga tcggaaatca cggccacggc agtaggatcg
     1921 ccgcccgctc caaatcctga aaacaccatt tgccccgcat attgtcccgt ggccatcacc
     1981 aaattcttgc tgccttcgac gtgcgctacg agcgacgacg cgggaaccag cgcgggtcgc
     2041 acaaatgcgc gcacgcttaa tccgtcttct gtctcttttt tggccagcga aatttgacgg
     2101 atcgtgcagc ccaattcgcg cgcataacca aaatcgacag cctcgaccgc cgaaatcggc
     2161 cagcacggaa tctgctcgct ttgcactcga acccgcaggc ccgcttgtgc aagaatcacg
     2221 agcttggcac gcgcgtcgta gccttccaca tcggccgtcg gatcggcttc ggcgtaaccc
     2281 agttcctgtg cttcccgaag cgccgattca aaactcgcgc ccgtcgcttc cattttcgtc
     2341 aaaataaaat tgcacgtacc attaagcacg ccggcaattt tgaaaagtct gtcgcccgcc
     2401 agcccatcct gaattcccat gatcgcagga attcctcccg cgaccgacgc gccaaattcg
     2461 aggcgtctgc cctcctcgcg cgcaagctcc acaagctcag gcccgctttc ggcaatcagt
     2521 tgtttattcg cggtgaccac ggatttcccg gcgcgcagcg ccttccgaat ccagtcgcct
     2581 gcgggcttga ttcccccggc cagctccaca aaaatatcga cgtccgagtt aagaacttcg
     2641 ttgatgcttt ccgtccaacg cacgctcgct ggaacccagt ccactttttt gcgcgccacg
     2701 tcgcgattga aaatatgcgt cagctcaaat gtctcgctcg cttcctggcc cagtattttc
     2761 gctacggacc tgcccactgt gccgaaaccg gcaatcgcga ccttcaattt tttccggcgc
     2821 accacgggac ttttctcagg aacggatttg gcgcgcgact ttattctcgg ggattttgcg
     2881 ttcttcgatt ttgccgccac tgcctcacct cgctgggaac ccgtaacagg atttcgccca
     2941 tcttcagaat ttatggggaa atccggttct gtagcgcccg ctttcaggcg ggcatgtggt
     3001 tgcatccacg gtccccgcct gaaggcgggc gctacaggcc ggaaggatta taaggtattt
     3061 tggtggcagg gccagtgaag tctgctgcca tgccgtcaag atgaaacttc cgggctggct
     3121 acggaatcca attcgcaatg gcgcaattct gatccgctag ccgcggatag agcctgcggc
     3181 gcaaccttat ggcacaaatt gaccagcggc ttccgcaaaa tgtcgaaggt gaattttacg
     3241 tcgacgaaag ttgcatcgac tgcgacgcct gccgccagat tgcgccggcc agtttccgcg
     3301 atcacggcgg caaatcgagc gtttttcatc agccggaaaa tcccggtgaa ctccaccgcg
     3361 ccttcatggc cctcgtcgcg tgccccacgg cttccatcgg caccgtctcc aatctgaatg
     3421 cgcgttccgg cgtagcggca tttcccgtgc acgtcgtgga caatgtttat ttttgcggct
     3481 tcactgcgga atcgagcttc ggtgcgtggg cttacttgat cgtgcgtccg ccggaacaag
     3541 gtggcaatgt gctcgtcgat tcgccgcgct tcaacggccc ccttgtaaaa cgcatggaag
     3601 aaatgggcgg cgttagcttg atgctgctat cgcaccgcga tgacatcgcc gatcatgcca
     3661 agtttgcagc gcacttcaaa tgcccgcgca tcctgcacga gggcgaaggc tcggccacgc
     3721 gcggcatcga acgcatcatc accggaaccg cttctgtgca gattgacgac gatctcttgt
     3781 tgatccccac gcccggacac acacgtggcc atcaggtttt tctctatcac aataaagttt
     3841 tattcaccgg agatcatctc tcgtggtcgc ccgcgcgcaa ttccctgaaa gctttccgcg
     3901 atgtttgctg gtattcctgg accgaacaaa ctcattccat ggaaaagctg ttggcctaca
     3961 attttgaatg ggttctgccc ggccacggcc gtatcggcca caccacaccg gaagaaatgc
     4021 acaccaaact ggaagattgt gtagccagaa tgaaaaagca gtaggttttc gtggcatagg
     4081 cttcagcctg tgggggtttg gccttgcaaa gatcaaatgc ctaaaattct taatttcgca
     4141 aaaggaccaa attcgacagg ctgaagagtc tgtgtgagaa ctgcagactc tttgtgggtc
     4201 gtggctttaa ccacgacata agtcctgcaa catgaatgcg gctttagccg ctgaagtttc
     4261 aattttgcag ttctcacaca gactctgaag cctaaactcg ctcgagcagt atcgcaatgc
     4321 cctgaccaac cccaatgcac atcgacgcca gcgcgtagcg cccttttatc cgttgcagtt
     4381 gccgcgccgc ggtcaaaacc aatctcgccc cgctcatccc cagcggatgc cccaacgcaa
     4441 tcgcgccgcc attgggattc actttctcgc tgtcatcagg caagccaagt tcgcgcgtaa
     4501 ccgccagagc ctgcgccgca aacgcttcat tcaattcgaa gacatcgaca ttgctcatcg
     4561 aaattttcca ccgcgcgagc agtttcctca ccgcgtaaat gggccctata cccataaaat
     4621 ccggcgccac gcccgccgct gctcctccca caacacgcgc aatcggcttc agcccaaaat
     4681 tcgcagccgc gtcttctgac gccacaaaca tggcgcacgc gccatcatta atgcctgaag
     4741 cgtttccggc cgtcaccgta ccgccttcac gaaacggtgt cggcaacttg gacagcgcct
     4801 caatcgtagt atccggacga atgtgctcat cctcatgcac cggcctgatc gttcccttcg
     4861 gccccggaat aggaacggga acgatttcct cgcccaggat cccggctttc tgcgctgctg
     4921 ctgccttttg ctgcgagcgc aaagcaaagc aatcctgcgc cgcgcgctcc accttatatt
     4981 ccgcggcaac attctccgcc gtctccggca tcgacgcgtt cccatacatt tttatcgttt
     5041 gcggattcgg aaaccgccag ccaagggtcg tatcgtaaat ttccgcgttt cgcgagaacg
     5101 gcgcagtggc cttgggcatc acaaacggcg cgcggctcat gctttccaca ccgcctgcaa
     5161 cagccagcga aatttcgtcg gcggtaatcg cccgcgccgc tactgtcacg gcctccatcc
     5221 cggagccgca cagccgattc acggtcattg cgggaatcgt gtgcggaaaa tggctaagca
     5281 agagcgccat gcgcgccaca ttgcggttgt cttctccaga ctgattcgcg cagccaaaaa
     5341 ttacttcgtc aaccgattcc caatcgctct tctgaaactg cgagtacaaa gcttttaggg
     5401 gaactgccgc caagtcatcc gcgcgcacgc tggaaagtac gccaccgtaa cggccaatgg
     5461 gagttctgaa cccttcacaa atgtatgcgg atgtcacgat ctgtgatttc cttccccaca
     5521 caaacttttt gcaagcacgc catttaaatt agatattgcg aaatgagccg aaagctgcag
     5581 agcgcccgcc caccgcagcg gacgagcgct ctcgaattcc ttcatcgtgc tacgccaatt
     5641 caaatgctcg ctgcctggtt acttggcagg tgtcggtgcc gaaggaacat cgctgccagg
     5701 tgtcgcctga atgctgacca cttcaatctc aaaaacgaga gtcgagttag gcccaatcac
     5761 gccgcctgcc ccgcgctctc catacgccag ctgcgacgga attacaacct gccacttgga
     5821 gcccgctggc ataagttgca gcacttccct gaatccggga ataacgttct tcacttcaaa
     5881 cggaaccggc tttccggctt gcgtggaact gtcaaactct tggccatcaa taaacgtacc
     5941 cttgtaattg cacaaaactg tgtcgctttc cacggccttt ttgccagtgc ctgccgtcaa
     6001 aaccttgtac tgcaatccat cgggcaaca
//
LOCUS       DASZMB010000061        10874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3910313, whole genome shotgun sequence.
ACCESSION   DASZMB010000061 DASZMB010000000
VERSION     DASZMB010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 10874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10874
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3910313"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..1006
                     /locus_tag="VG272_02575"
     CDS             212..1006
                     /locus_tag="VG272_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12594.1"
                     /translation="MKHLAGQKNQTAKDSSQVVATPTHAPAHSGELLRYYDAMSAALG
                     PMRWWPANTPFEVIVGAILTQSTAWGNVELAIANLHSAQLLTPSAMLRVRTSRLATLI
                     RPSGYFRQKAKKLKAFVRFLQTGYKGSLARMFQTPTAELREKLLEVHGIGPETADSIL
                     LYGGNHPIFVVDAYTHRILGRHGITDGKAAYEKVRGLIEDSIPRQAELFNEFHALIVN
                     TGKNWCRKSAPRCEECPLRPLLPANSPFAVFNGFVQAKTSVSGSLA"
     gene            1003..3213
                     /locus_tag="VG272_02580"
     CDS             1003..3213
                     /locus_tag="VG272_02580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF12595.1"
                     /translation="MSTSRKTQVFLAIGLVTIALLLAAVFTLGSLTLPFQPTDQVVLY
                     ALSMFVVTALVVFLFILARTLVRLGAERLAHKPGSRFKTRMVLGAIAISFLPVIVLFF
                     VSYALLNRTLVLWFPRPLEIATESSRALVNEMSRADADRMAGIAKEVAGTATADSARD
                     TMHAFDQTFARGADLAWEVNESGRPFAVAVNPNLQTQPRVAGGEPSAHPDPPALVRQI
                     PGGAELWEYNGKVFLAGRAKSANGFLLAGRIVPDDFQERIKEISTQTAAYEMQRQHLR
                     TYKNQMLATLLLFTILLIFAATWAAFFLSKQVTVPIQALAHATQAIIEGNYETRVNVQ
                     ANDELETLVNSFNRMTEQLGDSRRQIDIFTQNLQQAVQELERRRTLMETILENIPTGV
                     ISVDETESIRRVNPAAVRMFGESAKQAQNFVELLGEEAAHFVHILMRRSLRLGTATQE
                     LEFSATGRLLRAAVTVSSLGPLRSNRGYVVVVDDLTDLLRAQKAAAWQEVAQRIAHEI
                     KNPLTPIQLSSQRLSRYLERHRESLSKNDPPELVKLVGECSGLIEREVRVLESLVGEF
                     SQFARFPTAKLSPASPNEIVMEALSVFDGRLEGIQIRKDLAPDLPVIRADSELLRRVL
                     VNLIDNAAESMEGSAVKDLLLRTRFNNEREIVEIIVSDSGHGISPEDKDRLFLPHFST
                     KDRGTGLGLAIASRIMAEHHGSIRAEDNLPTGAKFILQLPMAEVASAPMTPRIG"
     gene            3226..4578
                     /locus_tag="VG272_02585"
     CDS             3226..4578
                     /locus_tag="VG272_02585"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWF12596.1"
                     /translation="MANSILIVDDEQGIREVLSSILTEEGYEPVAVPSGEECLSLLEK
                     RSIDLVLLDVWLEGIDGLETLERMRKNGVDAMVVMISGHASIETAVRATKLGAFDFIE
                     KPLSLEKVILVIKNALEVHRLAQENRQLREELGGRTVLQGETVPMRALRQQIALTAPT
                     HGRVLIYGESGTGKELVARALHAASLRAERAFVEVNCAAIPEELIESELFGHIKGSFT
                     GATENKVGKFEKADGGTLFLDEIGDMSLRTQAKVLRVLEEQRLEPVGSNQTVTVDVRV
                     IAATNKTLEDEIAHGRFREDLFYRLNVIPFTLPPLRERIEDVPVLARYFMLEFAASYG
                     RKPKEFGEAALGVMARYSWPGNVRELRNLIERLMIIVPHDKIEPLHLPAELFRAGSRM
                     TQQPNATLHEARSAYEREFILRKLEENQWQMTRTAEALGLERSHLYRRMRALGIKAEE
                     "
     gene            4794..4867
                     /locus_tag="VG272_02590"
     tRNA            4794..4867
                     /locus_tag="VG272_02590"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4826..4828,aa:Cys,seq:gca)
     gene            5019..5471
                     /locus_tag="VG272_02595"
     CDS             5019..5471
                     /locus_tag="VG272_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF12597.1"
                     /translation="MVEDNYRVPSVHAIRTLIADDNEMVRTQIGEILTTNPALEICGR
                     SKDGSEAVDMVRELRPNFVILDISMPGLNGFEAASQIRQLMPSVKIVILSMHESPQLE
                     AAALRAGADAIVSKRLAAESLISAIDRLFFADSPAQKIVTEEQVRNSG"
     gene            complement(5590..6504)
                     /gene="era"
                     /locus_tag="VG272_02600"
     CDS             complement(5590..6504)
                     /gene="era"
                     /locus_tag="VG272_02600"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HWF12598.1"
                     /translation="MAEKFKSGFVAIVGRPNAGKSTLVNHLVGEKVAIVTSRPQTTRN
                     RIQGIVNRENAQLVLIDTPGLHRPESALGRQMMGEVEAAIEAVHIVALLLDASEDFGV
                     GDRRAVERLQRVEGTRFLLLNKIDRIPRAELLPIIEKLSKLGKFDEIIPISALKGDGV
                     ERAMDKFIEYLPEGTPQFPTDQYTDQPERFLAAEIVREKAMASTTQEVPHAVAVICDA
                     FEETPKLIRIRATIYVDREGQKGILIGKGGGALKQIGTSARKELETILGARIFLELYV
                     KVLKNWRENPQIVRQLDWHWQLEQMAGE"
     gene            6665..7372
                     /locus_tag="VG272_02605"
     CDS             6665..7372
                     /locus_tag="VG272_02605"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HWF12599.1"
                     /translation="MPATPHIEHHFTASESVRDVVIGMSDGLTVPFALAAGISGAIAS
                     NPHASTLVVTAGLAEIAAGSIAMGLGGYLAAKTDQEHYASERDREVSEIVELRDVEID
                     EVAKVFRDYGLTEAELAPAVRAITGNEKSWVDFMMRFELGLEEPQPKRARISAMTIAF
                     SYIVGGLIPLAPYILLSDVRTGLYFSVGFTLLALAIFGWVKGRFTGIHPLKGALQTVV
                     TGGLAAGAAFLIAKLIS"
     gene            complement(7545..8822)
                     /locus_tag="VG272_02610"
     CDS             complement(7545..8822)
                     /locus_tag="VG272_02610"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWF12600.1"
                     /translation="MMTLSYVLAISALGVGLAIFSYLDRIYRELGHVNKGRVRAHLDL
                     FENEIEPHFKIERTRATIAFRLLANLWLVLVTAETARGVIVFVPGTWDALLQALVYVL
                     VEVLLLSQFLPDFLLARTSGRWLIRLSLVIRAFLWIVLPFRVLLEIAISLARISDDQH
                     ADGAPTQEESIEALVDAAEQEGILDPGEVQMIEQVVEFSDKRVLEFMTPRPEVVAIAS
                     TATIEQLRTLLIQSKFSRIPVYENSLDDVTGVVFARDVLRIPESEAATRTVRELARPA
                     FFIPETKQGNDLLKEMQRRNQQMAIVVDEHGSVAGVVTVEDLVEEIIGEIGEDDRVPA
                     PEAVREPDGSMVLRGSVSVEKVQELFGVEWDISGEESATTIGGLLNHVAGHVPAAGEH
                     LDYGGLRFEILEANQRKVLRLRGRRLPAAVAAT"
     gene            complement(8819..9244)
                     /gene="ybeY"
                     /locus_tag="VG272_02615"
     CDS             complement(8819..9244)
                     /gene="ybeY"
                     /locus_tag="VG272_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HWF12601.1"
                     /translation="MIVNRQRRVPVSIPTLQQFHERVRRELGFAPESVTIQLISDVAM
                     ARLNETYRRKQGPTDVLSFPAEGNRPASNAEYVGDIAISPETARRNARRFARTLPVEM
                     RILILHGMIHLAGFDHETDHGEMGRFERRLRKRLGVNGR"
     gene            complement(9370..10380)
                     /locus_tag="VG272_02620"
     CDS             complement(9370..10380)
                     /locus_tag="VG272_02620"
                     /inference="COORDINATES: protein motif:HMM:NF014605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HWF12602.1"
                     /translation="MRRTVQIAGGVESLFGTLDENVKLLESALHITTHLADDHLSIEG
                     EANQVDRAVRIVNEYNQLFREGRQMSHGDVRNMIKVATQDSKTTLRQVLEPGAPSRPR
                     IFGKKNVTPKSTNQREYMEAVEKHDMTFAVGPGGTGKTYLAVAMAVSALLTKQVDRII
                     LARPAVEAGERLGFLPGTLQQKIDPYMRPLYDALYDMLDADKLERFLEKGIVEVAPLA
                     FMRGRTLNDSFVILDEAQNTTSEQMKMFLTRLGFNSKAVVTGDVTQIDLPQGRKSGLV
                     EALDVTSRIDGIAFIKFDERDVVRHNLVQQIIKAYEEFGGGDNRRTAPADNGKPESSN
                     GH"
BASE COUNT         2411 a         2974 c         3031 g         2458 t
ORIGIN      
        1 aatgggaact ccacgggcgt acacacaccc ggcaagatta aaagtagcag aatgcggaga
       61 gcggccatgg ccccattttt gctagtcgta ttgaaagggg cactaagacc gaacctgtcc
      121 attggtacag gtacttttcc agctcgcaca gcgacaaatc ttgactagta cttcggtgat
      181 cgctatcttc cgagccatga gtagaatagc tgtgaagcat ttggcaggac aaaaaaacca
      241 gactgcgaaa gacagttcgc aagtcgtggc aactccgacg catgctcccg cacattccgg
      301 cgaattattg cgctactatg atgccatgtc cgccgcgctg ggcccgatgc gctggtggcc
      361 ggccaacaca ccatttgaag tgattgtggg ggcgatcctg acgcaaagca ccgcatgggg
      421 taacgtcgaa ctggccatcg ccaatcttca ctcggcgcag ctgctgacgc catcggcgat
      481 gcttcgcgtt cgaacttcgc ggctcgccac tctgattcgg ccctcaggct atttccggca
      541 aaaagctaaa aagctgaaag catttgtgcg atttcttcag actggctata aaggttccct
      601 ggcacgcatg tttcagacgc ccacggctga actacgcgaa aaacttcttg aggttcacgg
      661 gatcggtccg gagactgccg attccatctt gctgtatggc gggaaccatc ccatttttgt
      721 ggtggacgcc tacacgcata gaattttggg gcggcacgga attaccgatg gcaaagcggc
      781 ttatgaaaaa gtgcgcggcc tgattgaaga ttcgattcca cggcaggccg agctcttcaa
      841 tgaattccac gcgcttatcg tgaacaccgg gaaaaactgg tgccggaaaa gcgctccgcg
      901 ttgcgaggaa tgcccgcttc gtccactgct ccctgcaaat tctccattcg cagtttttaa
      961 tggattcgtg caggcaaaga catcggtttc cgggagcctg gcgtgagcac gagccgcaaa
     1021 acgcaggtat tcctcgccat tggcctcgtg accatcgctt tgctacttgc cgcggttttt
     1081 actttgggtt cactgacact tcctttccag ccgaccgatc aggtcgttct gtatgcgttg
     1141 tccatgttcg tagtgacggc gctggttgta tttttattca ttctcgcgcg aacccttgtt
     1201 cggctgggtg cggaaaggtt ggcgcataaa cccggatcgc gatttaaaac acgaatggtt
     1261 cttggcgcaa tcgccatttc gtttcttccg gtcattgtcc tgtttttcgt tagttatgcg
     1321 ctgttgaaca ggacgctggt tctctggttt ccccgtccac ttgaaattgc aacggaatcc
     1381 agccgcgcgc tggtcaatga aatgagccgc gcggatgccg atcgtatggc cggaatcgcc
     1441 aaggaagttg caggcacggc tactgcggat tccgcgcgcg acaccatgca cgcattcgat
     1501 cagacctttg cgcgcggcgc tgatctggcg tgggaggtca atgagagcgg gcgcccattc
     1561 gccgtcgctg tgaatccaaa cttgcaaacc cagccacgcg tcgctggcgg cgagccaagt
     1621 gctcatcctg acccgcccgc actggtcaga cagatacccg gtggcgcgga gctatgggaa
     1681 tataacggca aggttttttt ggctgggcgg gccaaatcag caaatggatt ccttctcgct
     1741 ggaaggatcg ttcctgacga tttccaggaa cgaatcaagg aaatttccac gcaaaccgcc
     1801 gcctacgaaa tgcaacgcca gcacttgcgg acgtacaaga accagatgct cgcgacgctg
     1861 ttgctgttta caattcttct gatatttgcc gcaacgtggg ccgcattttt cctgtccaag
     1921 caggtcacgg ttcccattca agcgttagcc catgcgacgc aagcgatcat tgagggtaac
     1981 tacgaaactc gcgtcaacgt gcaagccaac gatgagttgg agacgcttgt caactcattt
     2041 aatcgcatga ccgaacagct tggcgacagc cgccgccaga ttgatatttt cacgcagaat
     2101 cttcaacaag ccgtgcagga actggagcgg cgccgcactc tgatggaaac aattctcgaa
     2161 aacattccta cgggcgtgat ctcagtggac gaaacggaat cgatccgtcg cgtcaatccc
     2221 gcggcagtac gcatgtttgg agagagtgcg aagcaggcgc agaatttcgt ggagttgctc
     2281 ggagaagaag cggcgcattt tgtgcacatt ctgatgcggc gatcgcttcg gctgggaacc
     2341 gcgactcagg agctggaatt ttccgcgaca gggcgtttac tgcgcgccgc ggttacagtg
     2401 agctccttgg gaccgctgcg atcgaaccgc ggatacgttg tcgtggtgga cgacctgacg
     2461 gatcttttgc gcgcgcaaaa ggccgcagcc tggcaggaag tggctcaacg tatcgctcac
     2521 gaaatcaaaa atcccttgac gccgattcag ctttcctcgc agcggctgtc ccgttacctt
     2581 gagcgacatc gcgagagcct ctctaagaat gatccgccgg aactcgtgaa attagtgggc
     2641 gagtgctcgg gattgatcga gcgcgaagtc cgcgtgctgg aatcgctggt cggagaattc
     2701 tcgcaattcg cgcgattccc caccgcgaag ctttcgcccg cgtcgcccaa tgaaatcgtt
     2761 atggaagcgc tgtctgtgtt tgacggacgc ctggaaggca tacagatacg caaagatctc
     2821 gctccggatt tgccggtgat acgcgcggac agcgaattac tgcgccgcgt gctggtcaac
     2881 ttgatcgata atgcggcgga atccatggag ggctcggcgg taaaagattt gctgctgcgc
     2941 acgcgattca acaatgagcg cgaaatcgtt gagatcattg tgtccgatag cgggcacgga
     3001 atttctcccg aagacaaaga ccggcttttc ctgccgcatt tttcgacgaa agatcgcggc
     3061 acgggtctgg gactggcgat tgccagccgc atcatggctg agcatcacgg ctccattcga
     3121 gccgaggaca atctgccgac cggtgcaaaa ttcatccttc aactccctat ggcggaggtc
     3181 gcttcagccc cgatgactcc gcgaatcgga tagctcccaa ctcttatggc caattcaatt
     3241 ttaattgtgg acgatgagca aggtatacgc gaagtccttt cgtcaatcct gacggaggaa
     3301 ggctacgaac ctgttgcagt accctcgggc gaggaatgcc tatcgctgct ggaaaagcgc
     3361 tcgatcgatc tcgtgttgct cgatgtatgg ctcgaaggaa tcgatggcct ggaaactctg
     3421 gagcggatgc gcaagaacgg cgtggacgcc atggtggtca tgatctcggg tcacgccagc
     3481 atcgaaacgg ctgtgcgcgc gacgaagctc ggcgcattcg atttcatcga gaagcctctc
     3541 tcgcttgaaa aagtaattct ggtcattaag aatgcgctcg aagttcatcg cttggcgcag
     3601 gagaaccgcc agcttcgcga ggagcttggc ggacgcacgg tgttgcaggg cgagaccgtg
     3661 cccatgcgcg cgctgcggca gcagatcgcg ctgacggctc ccactcacgg acgcgtttta
     3721 atttatggag agagcgggac gggcaaggaa cttgttgcgc gcgcgctcca cgcggccagc
     3781 ctgcgagccg aacgcgcttt tgttgaagtc aattgcgcgg cgattcccga ggagctgatc
     3841 gaatcggaat tatttggcca cataaaagga agcttcacgg gcgcgacgga aaacaaagtg
     3901 gggaaattcg agaaggccga cggcggaact cttttcctgg atgaaatcgg cgacatgagc
     3961 ctgcggacgc aggccaaagt tttgcgcgta ctggaagagc agcggctcga gccggttgga
     4021 tcgaatcaga ccgtcactgt cgatgttcgc gtgattgccg cgaccaacaa aactttagag
     4081 gatgaaatcg cgcatggccg tttccgcgag gatctttttt atcggctgaa cgtgattccc
     4141 ttcaccctgc cgccgctgcg cgaaaggatc gaggatgtcc cggtgctggc ccgctacttc
     4201 atgctggaat tcgccgcatc ctacgggcgc aagccaaagg aattcggcga agccgcgctt
     4261 ggcgtgatgg cgcgctactc gtggccggga aacgtacgtg aactgcgcaa tttaatcgag
     4321 cggctgatga ttatcgtgcc acacgataaa atcgagccgc tgcatcttcc cgccgagttg
     4381 ttccgcgcgg gttcgcgcat gactcagcag ccgaacgcaa cgcttcacga ggcgcgcagc
     4441 gcgtatgagc gggaatttat cttgcgcaag ctggaagaga atcaatggca aatgacgcgc
     4501 acagcggagg cattgggcct ggaacggagc cacttatacc ggcggatgcg agccttgggc
     4561 atcaaggccg aagagtaagc cacgggaaat actttttgca aggattcaaa tccagccaca
     4621 ccgccgcaat gcgacccggc ttgcataaac cagaatataa tttcctacgg atttcacctg
     4681 attgtctttc tttatagcat tatttaatct caacgcacct tggcgtcggg ggttcgagtt
     4741 ttgaatcgca aggaaccaat gtaaccagaa caaacgaacc gtattgtcac gaaggcgcgg
     4801 tcgccaagtg caaaggcaaa ggtcggcaga acctttatcg gcggttcaac tccgccccgc
     4861 gcctccaacc tcctccagac caaaacaatt cccgcatctt catcgatggg gccatttcga
     4921 gaatatttcc cttaacaatt ctggtctatc gtgtacgata ggtccaaggg acagattaat
     4981 ttgggaagcg tcaccgaacg attgtgcggg gattactgat ggttgaagac aactatcgag
     5041 ttccgtcagt ccatgcgatc cgcaccctga ttgccgatga caatgagatg gtgcgcacgc
     5101 aaatcggcga aatattgact acaaatccag ccttggaaat ttgcggccgc tcgaaggacg
     5161 ggtctgaagc tgtggatatg gttcgggagc taagacccaa ctttgtgatc ctcgatatca
     5221 gcatgccggg gctgaatgga ttcgaggctg ccagccagat acgccagcta atgccttccg
     5281 tcaagatcgt aattctcagt atgcacgaaa gccctcagct ggaggcggca gccttacggg
     5341 ctggcgctga tgctatcgtt tcaaaacggc tggccgcgga atccctgatc agcgcgattg
     5401 atcgcttgtt ctttgccgac tcgcccgctc aaaaaatcgt gactgaagaa caagtccgaa
     5461 attcgggata gttatttgtt tccgtttttt ggaactgttt ggtcaatttc tgcttcgatc
     5521 atgacggcga cgtgcacgcc ttccctaaat ttcaaaagct tgagcccgcc agaatcattt
     5581 caaggctggc tattcccctg ccatctgttc cagttgccag tgccagtcca gttgacggac
     5641 gatttgcgga ttttcacgcc agtttttgag aactttgacg taaagctcga gaaaaatcct
     5701 ggcgcccaat atggtttcca gttccttgcg cgcggaggtc ccgatctgct tcagcgcgcc
     5761 gccgcccttg ccgatgagaa ttcctttttg cccctcgcgg tcgacgtaga ttgtggcgcg
     5821 aatacgaatc agctttgggg tttcctcaaa tgcatcgcag atcacggcca cggcgtgagg
     5881 aacttcctgc gtggtggagg ccatcgcttt ttcgcgcaca atttccgcgg ccaggaaacg
     5941 ctccggttga tccgtgtact gatcggtggg aaattgcggc gtcccttcgg gaagatactc
     6001 gataaattta tccatggctc gctcgactcc atcgcctttg agggcggaaa tcggaataat
     6061 ttcatcgaat ttcccgagct tggataattt ttcgatgatt gggagcaact cggctctggg
     6121 gattcgatcg attttattga gcagcaagaa tcgtgtgcct tcgacgcgct gcaagcgctc
     6181 cacggcgcga cggtctccaa ctccaaaatc ttcgctggca tccaggagaa gcgcgacaat
     6241 atgcacggct tcgatagccg cctcgacttc acccatcatt tgccgcccca gcgccgattc
     6301 gggccgatgc aatccgggag tatcgatcag tacgagctgc gcattctcac ggttcacaat
     6361 accctgaatg cgattgcgcg tggtctgcgg ccgcgaggtt acaattgcga ccttttctcc
     6421 aaccaggtga ttcaccaatg tcgatttccc ggcattgggc cggcctacga ttgccacgaa
     6481 tcctgatttg aatttttccg ccatgcaggc atgatacccc gcgctgtgga gaaaggcatg
     6541 ctcccaatcg ctttagatgg gcaggcatat tcggcgacat gcgatggcgt gttacacttt
     6601 cccggcttga cgcctgttga gattcatcgg agagcctaat tttttatttt ttggagaaac
     6661 cgggatgcct gcgactccac acatcgaaca tcatttcacc gcgtcggaat cggtgcgcga
     6721 cgtcgtcatc ggaatgtctg acggcctgac ggtgcccttc gcgcttgcgg cgggaatttc
     6781 aggagccatc gcatccaatc cccacgcgtc aacgcttgtg gtcaccgccg gcctcgcgga
     6841 aattgcagcg ggatcgattg ccatgggcct tggcggctat ttggccgcga agaccgatca
     6901 ggaacattac gcttcggagc gcgatcgcga agttagcgaa attgtggagc tgcgcgatgt
     6961 ggagatcgac gaagtcgcca aagtttttcg cgattatgga ctgaccgaag cggaactggc
     7021 tcccgccgtg cgggccatca caggcaacga aaaatcctgg gtggatttca tgatgcgttt
     7081 tgaacttggg ctggaggagc cgcaacccaa gcgcgcgcgc atcagtgcga tgactattgc
     7141 tttttcctat atcgtcgggg gactcattcc tctggcgcca tacattctgc tttctgacgt
     7201 gcgaacggga ctttattttt cggtagggtt tacgctgctt gcgctggcga tcttcggctg
     7261 ggtcaaggga cggttcacgg gaattcatcc gctcaagggt gcactgcaga cggtcgtaac
     7321 cggggggctc gcggctggcg ccgctttttt gattgccaag ctcattagtt gagtgaaggg
     7381 cgaaaagttt taacacagca cggagaaaag ccaaagagga cacggagatt ttttcaggct
     7441 aaaatttcat cattggaaat attttctctc ccgcgcgttc ttttctccgc ttttctgtgc
     7501 gcattttctc tgcgggctct cggttaaagc ctttgtagtt ttcgttaggt tgcggcaacc
     7561 gccgcaggaa gccgccgacc gcgaagccgc aatactttcc gttgattagc ctcaagaatt
     7621 tcaaaacgca gcccgccata atccaaatgt tcgccggccg caggaacgtg acctgcgacg
     7681 tgattgagca ggccgccaat tgtggttgcg gattcttccc cgctaatgtc ccactccacg
     7741 ccaaaaagct cttgaacttt ttcaacggac acgcttccgc gcaggaccat gctcccatca
     7801 ggctcgcgca ccgcctcagg agcaggcacg cggtcatcct cgccaatttc gccgataatt
     7861 tcttcgacca gatcttcgac cgtcacaacc cccgcgacgg acccatgctc atcgacgacg
     7921 attgccatct gctgatttcg ccgctgcatc tctttcagca aatcgttccc ttgctttgtt
     7981 tctggaataa agaaagctgg gcgcgcaagt tcgcgaacgg ttcgcgttgc ggcttcgctc
     8041 tcgggaatcc gcagaacatc acgggcaaag accacgccgg tgacgtcgtc cagagaattt
     8101 tcgtaaaccg ggatgcgtga aaatttcgat tgaatcagca gcgtacgaag ctgttcgatc
     8161 gtcgcggtgg aggcaatcgc gacaacttcg gggcgtggcg tcataaattc cagcacgcgc
     8221 ttgtcgctaa actcaaccac ctgctcaatc atctgcactt cgcccggatc gagaattccc
     8281 tcctgctcgg cggcatccac gagcgcctcg atgctctcct cttgagttgg cgccccgtcg
     8341 gcgtgctgat catcggaaat gcgcgccagc gaaatcgcaa tttccagcag aacgcggaac
     8401 ggcagcacta tccaaaggaa cgcccgaatg actaacgaaa gacgaatgag ccagcgcccg
     8461 gaagtgcgcg cgagtaaaaa gtccggaagg aattgagaaa gcaggaggac ttcgaccaaa
     8521 acataaacga gggcttgcag cagcgcatcc caggttccgg gaacaaaaac gataactccg
     8581 cgagccgtct cggccgtgac cagcacgagc cagagatttg caagcaagcg aaatgcaatc
     8641 gtggcgcgag tgcgctcgat tttaaagtgc ggctcgattt cattttcaaa cagatcgaga
     8701 tgcgcgcgga cccgcccttt gttaacgtga ccgagttcgc ggtaaatcct gtcgagatag
     8761 gagaaaatgg cgagacctac gccgagagcg gagattgcca ggacgtacga caacgtcatc
     8821 atcggccatt cactcccagg cgttttcgca ggcggcgctc gaagcggccc atttctccat
     8881 ggtcagtttc atgatcgaac ccggcaagat ggatcatgcc gtgcagaatc aggattcgca
     8941 tttccacagg caaagtacgc gcgaagcggc gagcgttgcg gcgcgccgtt tccggcgaga
     9001 tggcgatgtc gccaacgtac tcggcgttgc tggcgggacg attgccttca gcgggaaagg
     9061 aaagcacgtc ggttggtccc tgtttcctgc gataggtttc gttcaggcgg gccattgcta
     9121 cgtcactgat caattggatc gttactgact cgggagcgaa accaagttcg cgtctcacgc
     9181 gttcgtgaaa ttgctgcaag gttggaatgg aaactggaac gcggcgctgg cggttaacga
     9241 tcattgaaat cagaataatt gtatggcgaa ctgtgcgcgg caggcgaacc tttcagcaca
     9301 tgcgatcatg ccgaaggcgc ggaaaagcct cgggtctgaa gacccgagct acagcctggc
     9361 tgctcacctt taatgaccat tggaagattc gggcttgccg ttatccgctg gggcggttct
     9421 ccggttgtcg ccccctccaa actcttcgta tgctttgata atttgctgca cgaggttatg
     9481 gcgcactacg tcgcgctcgt caaattttat aaatgcgatg ccatcgatgc ggctggttac
     9541 gtcgagcgcc tcaacgaggc ctgactttct accttgaggc aagtcgatct gcgtcacatc
     9601 gccggtgacc acggccttgg aattaaatcc cagccgtgtc aggaacattt tcatttgctc
     9661 gctggtcgta ttttgcgcct cgtccagaat cacgaacgaa tcgttcagcg tgcgaccgcg
     9721 catgaaagcc aggggcgcga cttccacaat tcccttttcc aggaagcgct ccagtttgtc
     9781 cgcatcgagc atgtcataga gcgcgtcata aagtgggcgc atgtagggat cgatcttttg
     9841 ctgcaaagtg ccgggaagaa atccgaggcg ctcgccggcc tcgactgccg gacgcgcgag
     9901 aataatgcgg tccacttgct tggtcagcag cgcggaaacc gccatggcca cggcgagata
     9961 agtttttccc gttccgcccg gaccgacggc aaatgtcatg tcgtgcttct ctaccgcttc
    10021 catgtactcg cgctggttgg tgctttttgg cgtaacattt ttctttccga aaattctcgg
    10081 gcgcgatggc gcgccgggct caagcacttg acgcaacgtg gttttggaat cctgagttgc
    10141 cactttgatc atatttcgca catcgccgtg actcatctga cgcccctcgc ggaaaagctg
    10201 gttgtactca tttacgattc ggacagcgcg gtcgacctgg tttgcttcgc cttcaatgga
    10261 gaggtggtca tctgctaaat gcgtggttat gtgtagcgcg gattccaaaa gtttaacgtt
    10321 ctcatctaga gtcccgaaca aggactccac tccgcctgca atttgtacgg tgcgtctcat
    10381 ggaagattca gttagccggg gtcccgcatt ccgccaaccg attaggaaac acctccgccc
    10441 tgcataaggg cagacaaaag cgtacttgaa agagacggag gggtcaagga tgcatggaag
    10501 aagttgcatc gaagatgtgg ggacatccgt ttgttggatt gcagattgct gcatggcgaa
    10561 cggaaaaagc tgcccgcccg aaaggatggg cagcctctac aaaagcgacg gcaaggtttt
    10621 acggtctagt aggggcctcg ccgtcccacg ctcgtcggga cggaaagggc ggctttctac
    10681 tttcgcaata aattgctcag tgatcggatg gcggcagaac cagcgaatcg acctcgcccg
    10741 tggaaacttc gacgccggat ccagaggact gcacatcgcc tcccacaagg atcaggtgat
    10801 tccctatcac gccagccgcg agcccgtgcc tgctgaccgg catggacggc atgatggacc
    10861 acgaatcgct cgcg
//
LOCUS       DASZMB010000062        19946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_345244, whole genome shotgun sequence.
ACCESSION   DASZMB010000062 DASZMB010000000
VERSION     DASZMB010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 19946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19946
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345244"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="VG272_02625"
     CDS             complement(<1..1013)
                     /locus_tag="VG272_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWF12603.1"
                     /translation="MNRNEQHDQKNGHMTAAVLYGSENLKIESIDIPRLAADEALIRV
                     KVALTCGTDLKVWKRGYHARMITPPAVFGHELAGVVETLGSDVNGSLKVGARVVPSNS
                     APCNVCLFCRKGQSNLCEDLLFNNGAYAEYIRIPGRIVRQNMLVIPEHVSFQDAAMVE
                     PLACVLRGIHETGIQPGDTTVVIGCGPIGLKFIRVLANRGVNVIALGKRPNQIRAAEQ
                     LGARAAFIAPDLSDAAQTVRELTEGGRGADSVIEAVGTSQTWEWALQMVRRGGIVNLF
                     GGCPRGTQVAVDPNALHYSEITIKSTFHHTPRFIREALESVSSGEIRSSDFVNAEIPL
                     TELP"
     gene            complement(1010..2113)
                     /locus_tag="VG272_02630"
     CDS             complement(1010..2113)
                     /locus_tag="VG272_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWF12604.1"
                     /translation="MSQSGIILETSHAANVVPSTMRAAVYRGKGNVVVETVPVPVIGA
                     GEVLIRVAACGICGTDLKKIEHGFVAPPQIFGHEVSGTVVAVGQGVTKWKLGDRVMSF
                     HHVPCGSCFYCERRLFSQCLGYKKVGLTAGFDPNGGGFAQFVRAMPWVAERGMIAIPD
                     DITFDEASFIEPVNTCIKAVEKARVGAGETVVVMGLGPIGLLLAMLSKLEAATVIGSD
                     PMVERRAKSISLGLDFAVDPREGKLADEINSRTHGRGADVVLVAVPIPGALKDALSLV
                     RPGGRVLLFAQNDPLMKIEFPAAAVGVEEKEILGSYSAAIDRAEEASNLIFTRKLPVG
                     NLISHRFSLEDMDRALKLAARPVGDSLKVVIHP"
     gene            complement(2110..2862)
                     /locus_tag="VG272_02635"
     CDS             complement(2110..2862)
                     /locus_tag="VG272_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12605.1"
                     /translation="MKILVTFAVQAEFAPWQRRRNFMRLPGEWPVFEAMFGGAQVRAV
                     LTGMGQDFALKAAKRSLGYRPDMCISTGLAGSLQNEYRPGDILAARLVSEVGEPVAVA
                     SHPELLSTAVDCGARQIERMATSKTLVSRVEQKRQLGSQAEAVEMESYTILAEAARCG
                     IPAVAIRAIGDTADFDMPYNFEDALNDQGQIRTMGIASQVLRRPSGLPALLKLARDSR
                     FASRRLADFLDVFAGTMADRLIPIEQDTVAAL"
     gene            complement(2879..3904)
                     /gene="hpnA"
                     /locus_tag="VG272_02640"
     CDS             complement(2879..3904)
                     /gene="hpnA"
                     /locus_tag="VG272_02640"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HWF12606.1"
                     /translation="MKTLVTGATGFLGSHVARALVERGEEIRVLVRASSDLRALEGLN
                     AERVQGDLRDRASLDRAMEGVQRVFHVAADYRLWAKDPTEIHESNVAGTRNLLDAARS
                     AGVTKFVYTSTVATIAVPRDGDLPNEKTQASVGEMIGEYKRSKFQAEQCALHAAEDGL
                     PVVIVNPTTPVGPGDWKPTPTGKIIVDFLNGRMPGYVDTGLNFVPVEDCAHGHLLAAD
                     DGRVGERYILGGRNLTLKEMLDILSLTSGRPSPRWKIPYAVAYAAGWVDMGVSRLLNR
                     EPQIPLEGVRMSRHRMFVDATKAERELGFAPGPIEAALDRAVSWYEANGYVAAGRTPV
                     VAGARAA"
     gene            complement(3901..5043)
                     /gene="hpnH"
                     /locus_tag="VG272_02645"
     CDS             complement(3901..5043)
                     /gene="hpnH"
                     /locus_tag="VG272_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HWF12607.1"
                     /translation="MRYPIKMTANLAKYVAGQRFRGTKKFPMVMMLEPLHACNLTCTG
                     CGRIREYKSTINEIMTVEQCMAASDECGAPIVSICGGEPMIYPEIGRLSKEILDRGRH
                     IILCTNGMFIRKRLHEFKPTSSFFFNVHLDGLERTHDLCVERDGVFREAIEGIKAAKA
                     AGFLVCSNTTIYKETDLNEIADLYGYLDTLGVDGYMLAPAYGYSAVQTSDIFMTRADI
                     RQKFREAVALLEKYNLMVSPIYLDFLRGERELECTAWGTPTYNPRGWKGPCYVITDNH
                     YGSYREMIDQTPWENYGPGNDPRCADCMMHVGFETTPVLGAKRNLGDTWTMLKWQLGG
                     HMGGNPDTNASKTPPTNGHSGGGSTMMPPPPTNSDLVSSDGRLRVL"
     gene            complement(5053..7062)
                     /gene="shc"
                     /locus_tag="VG272_02650"
     CDS             complement(5053..7062)
                     /gene="shc"
                     /locus_tag="VG272_02650"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HWF12608.1"
                     /translation="MALGTRVQFDQSGESRSAGVLAALSERLSDTIDRGVNYLLSLQV
                     SEGYWIGELEADTTLESDYIFYLQIINKANPERIAKLANYVRRRQLEDGGWNIYFGGP
                     SELNATIKAYVALRLAGDAPDCENLRRAAKRIHELGGLESANSYTRLYLALVGAVGWD
                     MVPAIPPELMLLPNWFAINIYEMSSWTRGIVIPLTILYAHKPRFSVPGGIIVDELYRD
                     PSKKALALSWDKELFSWRNLFLALDRGLKLYERVPWKPLRQRALRQAREWMLEHLERS
                     EGLAAIYPAMMNAIFALVALGHSPDDPLTAREINEFSRFEIEEGDTIRVSPCVSPVWD
                     TAIAMVALEEAGLPPNHPALVEAAAWLLKNQICGSGDWMIKTPGVMPGGWAFEFRNDF
                     YPDVDDTAFVLMALQRVDYPDKARMEAAVRTGLTWLLAMQNRDGGWGAFDHNNDRQFL
                     CHIPFADHNAMIDPSTADVTARVVECLGRYGWPATHPAIERAVRFLLKDQTEEGPWFG
                     RWGVNYVYGTSGVLRALETVALTARDYCQRAVGWLRSVQNSDGGFGESIASYYDPALK
                     GKGNSTASQTAWGLIGLLAASEVSDPAIARAVTHLVDQQQADGSWAENEFTGTGFPCV
                     FYLKYHLYRNYFPLYALTRYRNTVSGIQQFCGLKVRPQEFDRQIA"
     gene            complement(7330..8355)
                     /gene="lpxD"
                     /locus_tag="VG272_02655"
     CDS             complement(7330..8355)
                     /gene="lpxD"
                     /locus_tag="VG272_02655"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HWF12609.1"
                     /translation="MARTMKELADFLGCPLEGDGAVRVSGVASTVSARAADLIYVESP
                     RHLDRAAASASMCVVIAPGLTLSGKVLLRAPKPKLAFARAAEWLIPATPIAKEIHRTA
                     IIASSAQIAAGVSVGPYAVIEEDVQIGSGTEIGAFCFIGRGSTIGESCRFYPRVTLYS
                     GVRIANRVILHSGTVIGSDGFGYVAEGGKHRKFPQVGEVEISNDVEIGANTTIDRGSL
                     DRTVIGSGVKLDNLIHIAHNVSIGENTVIAAQTGISGSVIIGKNVTLGGQIGMADHCE
                     IKDGAVIGAQAGILPGKIVRSGGILWGTPARPLEKFKEQFAWISRLPALAERIKRLES
                     IVNAKDE"
     gene            complement(8432..9439)
                     /locus_tag="VG272_02660"
     CDS             complement(8432..9439)
                     /locus_tag="VG272_02660"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWF12610.1"
                     /translation="MLGRKRWPNREPIVSRVTNHESRVTIFPRSRYNPSVQQRLEYAA
                     LWLLLKIIGTLPRPLARFVGARTAAFLFLLRPGLRAAATENLQLAFPTWNKKQRQAAI
                     RGMVRQLGWMGAEFAHFPHYTKNNIERTVLIEGFENFASAQARGKGVLFLTGHMSAWE
                     MAPFAQALYGNPLHFLVRPIDNPFVDELITKYRCSHGNQPIDKSQSARAVLKVLAAKG
                     TVGILADQNTQTTEGVFVDFFGIPACTTAGIARFALHTDAAVVPGFIHWDGATRKYRL
                     CFEPEVELTRTGDDATDIRENTQRFTRVIENYVRKYPDQWLWVHRRWKTHPTGEPSVY
                     K"
     gene            9593..11233
                     /locus_tag="VG272_02665"
     CDS             9593..11233
                     /locus_tag="VG272_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWF12611.1"
                     /translation="MNSTSPIRSRAAEEGSFQIPLWGVPFILLFVYVCIFSGLSALGV
                     VGPDEPRYAAIARAMAATGDWITPRLWGSPWFEKPVLYYWGAGIAMRIFGVGEFAARL
                     PSALAALLAVIAIAWTALRSYGIAACWFSLLMLPTSVAIIAFSRAASPDMLFAGFLTA
                     AMAVASEMLQKPRPGAVLRIAFGFFLGAAVLAKGPAAVILAGGATLFWAALSRQWTAP
                     FRFLHPLIIAVFCLTALPWYVLCALRNPDFLRVFIWQHNFQRYLTPVFEHRQPFWFFG
                     VVFLVAVLPWLPFLISSISDANSTLRSTEWRDSPALFIGSWSLFTLVFFSLSQSKLPG
                     YILPGIPPAVLLLAASAARRLQQKSSTLSNLAIAIGLLFPIGIAVALLQWNHAPNIDA
                     QLHAHIARGFQIALGAAVVLAAIIIGYAFRRKINEAIFVAASTTALLILLANRLVLPG
                     TDTLVSLRPAAMWAIQTHSLGADDVAVYALPRAYGYGLNYYFNATLPEWTPENTNAKI
                     LFCGRDALNSPQLESLRGKAKFPATGDDKVFLVLLNSD"
     gene            complement(11253..12200)
                     /locus_tag="VG272_02670"
     CDS             complement(11253..12200)
                     /locus_tag="VG272_02670"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWF12612.1"
                     /translation="MDFISEFRHPTYWYSKLAIGILAVVFFGLLITGAISGFAVYRME
                     SPSRRNSEINLQDFPGHPEELHFNVNGGVTRDAWFFPGLKTAPTIILCHAYGSSRGEL
                     LTLASGLQDQQYNVLAFDFSAHGSSAGHSTLGLQEVGQLRAAIDAVANRGDVDVSRFG
                     LWGANMGAYVALAEATNDPRVRAIVAESAYKSPNEMVALQVSRLGLGSVPFVTGMSQM
                     IFGWVNSQYRNVQPLDTRIGKLSGVPQLYLESPEDPRLAASTSQLFRMSPEPHEQAML
                     PHGNYGGMADEEKRNYENRIVSFFLVNLPISGDPLPQIR"
     gene            12418..13380
                     /locus_tag="VG272_02675"
     CDS             12418..13380
                     /locus_tag="VG272_02675"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWF12613.1"
                     /translation="MQIVDLRQLTSRQLEPLLAEEALQWREDLRWDYHSSTELIKKFV
                     DARSLTGSAILENGKPVGYAFYVLEEHKGLVGGLFVSPAYSQQDLSHQLLTDMLNTLS
                     GIPKLERVEAQLIPFGYQLDPVLSEYGFRLYMRQFMIAQLDQHTEVTLDVLPGLIREG
                     PGMGLILEHWDHRYFEQCARLIQLSYASHVDGEINDQYRSESGALRFLKNIIILPGCG
                     QFQPEASFILRAPYENQLVAVILNSRVASGVGHTTQICAMPGYQGRGLGRRLMEASLQ
                     ALRVRNFHTVSLTVTSGNERAVRLYERLGFATVKKFAAGVWTAF"
     gene            13535..14551
                     /locus_tag="VG272_02680"
     CDS             13535..14551
                     /locus_tag="VG272_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12614.1"
                     /translation="MRRTSIPALALLSLLIPAGALRADEIRLKDGTKIEGTIVGFESD
                     SFRVETSYGFALIRKDKVADINITASKKDSDSKTKSAASFDSTAPAAPPVTPAVVKQP
                     ATSNNSSKEGNGTAQDRSASSAGVTSSAASFKVDLAAPKVKPVAVSQVPPPAVTPAAS
                     ASASMAPLPAAPSTPPPAPEPVVIRDEIRGNQYVNVTYGFQMYKPPSWELIPGARKAL
                     PDAVAALGTSDQTTLLVIGREHAKDSLDAHAAKTGKALSDVYENYRQISSRQLSVAGF
                     PAVEQRARGSADGRDWSVTLLTLVKGGEAYTLLGMTWADSDLIQVQENVIAKAVNSLT
                     FAAP"
     gene            complement(14681..15700)
                     /locus_tag="VG272_02685"
     CDS             complement(14681..15700)
                     /locus_tag="VG272_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWF12615.1"
                     /translation="MDRRRFISIAGGAGLGILLPYAVYRSLAYGFGAANVDVKGYEKF
                     GAEAALRAIAPVDQFYVTSSHGEPAVDVHKWSLTIDGLVTDPLHFDYDDIRKLLPYET
                     TLTLECISNEIAGGLIDTANWRGTLLHPLIERAGPKANAKYAILYAAEGYTTGHTIER
                     LSRPANFLAYDMDGEPLTRIHGFPLRVLMPGIYGMKTPKWLTRIELVDKSHLGYWEWM
                     GWSNEGERQLQSVITDPHGSTQIAGQSFVVTGWAIANAAGVSKVEISTDGGTTWNACT
                     IFSNPMPSQVWAFWRYVWANPQSGKHEIQVRAVDGNGKLQTSSKSGEWPDGATGYHTV
                     EVNVG"
     gene            complement(16287..17705)
                     /locus_tag="VG272_02690"
     CDS             complement(16287..17705)
                     /locus_tag="VG272_02690"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HWF12616.1"
                     /translation="MAKKKTKHAKKDRPRAHHKSGVAVAGLHLLSDAELRRIAEKIFK
                     FSDADETEVEIGMTSDALTRFANNTIHQNVAEQVLNVSVRTVLDGRTARATTNKTEDD
                     SLRRVVETAKTLARSQPRNPDLLPMPGPQKYQKVARYFENTAYATPADRARAVVKATE
                     MAEKNKQTAAGIFSTGVTQFAIANTRGLFASHRQTRAEFSITILESDSSGWAKSNSPD
                     LAQIDPVALARNASEKSAASRKPSEVAPGRWTVILEPSAALDLVGFLFYDFTGTAMWD
                     QRSCFTKRMGKRILGENISLHDDAYHPLQSGAPFDGEGIPRQKILLVDKGVPSDLVYS
                     RATAKKMKAKPTGHGLPLPNDMGEAPMNLVFSGGNSSLDEMVRSTDNGILVTRVWYIR
                     DVDPYEKVLTGMTRDGTFLVRDGKLAGGLRNFRFNQSVLEMLSNVELLGPSVRAAGEE
                     SFDMVVPSMKVRNFHFSEVTKF"
     gene            complement(18254..19723)
                     /locus_tag="VG272_02695"
     CDS             complement(18254..19723)
                     /locus_tag="VG272_02695"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HWF12617.1"
                     /translation="MMRDFANWALETAKVRGATYADARVMDIRLRDLSTKNGQVGTLS
                     ESESFGIGIRVIAQGSWGFASTDRLTREGIAACAAQAVSIAKASALAKLHDVEVEMVP
                     VEAYRDTWQNPYVKDPFQIPIERQLDLLLAADREMSRIKGVTVSEASMTLRRIEQFFA
                     SSIGSAIHQIKMQSGAGICATAFAGNEIQKRSYPNSFGGQHMLSGYELIESLDLVGNA
                     PRVAEEAVALHSAAQSPAGAHTLILDSSQLGLQIHESIGHPIELDRVLGMEANFAGMS
                     FLTLEKLNKLQYGSEIVNVVADARLEHGPGLGTFGYDDEGVPAQCTPIISDGLFTGYL
                     SNRETAISVGEKTSGGTMRAESWNRLPIIRMTNISILPGAWRFDDLIADTDDGVYMET
                     NRSWSIDDRRYQFQFSTEIAWEIKGGKKKRMLKNPSYSGITTEFWNSCDAICSREDWT
                     LWGTPNCGKGQPMQTMGTGHGAAPARFRNIKLGAAFAKS"
BASE COUNT         4401 a         5906 c         5049 g         4590 t
ORIGIN      
        1 ggaagctcgg tgagcgggat ttccgcgttc acgaaatcgc tggagcggat ttctccgctc
       61 gacacgcttt ccagcgcttc gcgaatgaag cgaggtgtgt gatgaaacgt ggacttgatc
      121 gtgatctccg aataatgcag cgcgttcggg tccaccgcca cctgcgtgcc acgcgggcag
      181 cctccgaaaa gatttacgat tcctccgcga cgcaccattt gcagcgccca ttcccaggtc
      241 tgcgaggtac ctaccgcttc aattacggaa tcggctccgc gtccaccttc cgtcaattcc
      301 cgaaccgttt gtgccgcgtc gctcaaatcc ggcgcgataa acgccgcgcg cgcgccaagt
      361 tgttccgccg cgcgaatctg attcggccgc ttcccgagtg caatcacgtt cacgccgcga
      421 ttcgccaaaa cgcgaataaa tttcaagcca atgggaccgc aaccaatcac cacagttgta
      481 tcgccgggtt gaataccggt ttcgtgaatt ccgcgcaata cgcaggccag aggctcaacc
      541 atcgccgcat cttgaaacga aacgtgctcc ggaatcacca gcatattctg ccgcacgatt
      601 cgtccgggga tgcggatata ttccgcatac gccccattgt taaacagcaa atcctcgcag
      661 agattgctct gccctttgcg gcagaaaaga caaacattgc acggcgccga attggagggc
      721 accacgcgcg ctcccacctt caggctgcca ttcacatcgg aaccgagagt ttcgaccact
      781 ccggcaagct cgtgcccgaa tacagcgggc ggcgtgatca tacgtgcgtg gtagccgcgc
      841 ttccagactt tcaaatccgt gccgcaggtt aaggcgactt ttacgcgaat caacgcctcg
      901 tccgcggcga gcctgggaat atcgatgctc tcgatcttca gattctcgct gccatataaa
      961 acggcagcgg tcatgtgacc gtttttttga tcgtgctgtt cgtttctatt caaggatgaa
     1021 tcaccacttt tagggaatcg cctaccggac gggccgccag tttcagcgcc cggtccatgt
     1081 cttccaaaga aaaccggtgc gagattaaat tgccgaccgg caacttcctg gtaaaaatca
     1141 gattggaggc ttcttctgcc ctgtcaatcg cggcactata gctgcccaga atttcctttt
     1201 cttcgacgcc caccgctgca gccggaaatt caatcttcat caacggatcg ttctgcgcaa
     1261 acagaagcac acgtccgccg ggccgcacca ggctgagagc atccttcaga gcgccaggaa
     1321 tcggcaccgc aaccaaaacc acatccgcgc cgcgcccatg cgtacgcgaa ttaatttcgt
     1381 ccgcgagctt cccttcgcgc ggatcgaccg caaaatcgag accaagagag atactcttcg
     1441 cgcggcgctc caccatggga tcgctgccaa tcaccgtcgc ggcctccagc ttggagagca
     1501 tcgccagcag cagtccgatg ggcccaaggc ccattaccac aactgtttct ccggcaccca
     1561 cgcgcgcttt ttccacagct ttaatgcacg tgttcactgg ttcgatgaat gacgcttcat
     1621 cgaaagtgat gtcgtccgga atggcaatca tgccacgttc cgcgacccaa ggcatggcac
     1681 gcacaaattg cgcaaacccg ccgccattag gatcgaaacc cgccgtcaac ccaacttttt
     1741 tgtaacccag gcactgcgaa aacaagcgcc gttcacaata aaaacacgac ccgcacggaa
     1801 cgtgatgaaa gctcatgacg cggtcgccca gcttccattt cgtaacacct tgacccacgg
     1861 cgacaaccgt tcccgaaacc tcatgaccga aaatctgggg aggcgcaaca aatccgtgct
     1921 caattttttt caagtccgtg ccgcaaattc cgcacgcggc cactcgaatc aacacttctc
     1981 ccgcaccgat caccggcaca ggaaccgtct cgacaaccac gtttcccttg ccgcggtaca
     2041 ctgcggcacg catggttgac ggcactacgt ttgccgcatg tgaagtctcg agaatgattc
     2101 ctgattggct catagcgcgg ccacagtatc ctgttcgatc ggaatcaggc ggtcagccat
     2161 cgtccccgcg aacacatcaa gaaagtcagc cagccgacgt gatgcaaatc ttgagtcgcg
     2221 agccaattta agcagcgcgg gcaagcccga tggccttcgc aaaacctggg acgcgatccc
     2281 catcgtgcga atctggccct gatcgttcag cgcatcctca aaattatatg gcatatcaaa
     2341 atccgcggtg tcgccgatgg cgcgaatcgc aaccgctgga attccgcacc gtgcagcttc
     2401 ggcgagaatt gtataacttt ccatttccac cgcttccgcc tggcttccca gttgccgctt
     2461 ttgttccact cgcgaaacca gcgtcttcga tgttgccatg cgttcaatct gccgcgctcc
     2521 gcaatccaca gccgtcgaga gtagttccgg atggctcgct acagccacag gctctcccac
     2581 ttcgctaact aaccgcgcgg ccaaaatatc tcccggacga tattcattct gcagagatcc
     2641 cgcgagcccc gtcgaaatgc acatgtccgg acgataccca agcgatctct tcgctgcttt
     2701 cagagcaaag tcttgtccca tcccagttaa taccgctcgg acctgtgcgc cgccaaacat
     2761 tgcttcaaat accggccatt ctccgggcaa ccgcataaaa tttctgcgcc gttgccacgg
     2821 ggcaaattcc gcttgaaccg caaatgtgac caaaattttc atgaaccctg acgctcactt
     2881 acgccgctcg cgcgccggca acaacaggcg tgcgtcccgc ggcaacatat ccgttcgctt
     2941 cataccagga gaccgcgcga tcaagcgctg cctcaatcgg cccgggcgca aatccaagtt
     3001 cccgctcggc tttcgttgca tcgacaaaca ttctgtggcg cgacatccgc acgccttcca
     3061 gcggaatctg gggttcgcga ttcaggaggc gcgatactcc catatccacc caaccagcag
     3121 cataggctac cgcgtatgga attttccacc gtggcgacgg ccttccgcta gtcaaagaaa
     3181 gaatgtccag catttctttc agcgttaaat tgcgcccgcc taaaatatag cgctcgccaa
     3241 cgcgaccatc atcggcagcc agcaagtgcc cgtgcgcgca atcttccacc ggcacaaaat
     3301 tcaatcccgt gtccacatac ccaggcatcc ggccgttcaa aaaatcgacg ataatctttc
     3361 cagtcggtgt cggtttccag tcgccagggc ccactggcgt cgtcggattg acaatcacaa
     3421 ccggcaaccc atcttccgct gcatgcaacg cacattgctc ggcttgaaat tttgaacgtt
     3481 tatattcgcc aatcatttcg ccgactgatg cttgcgtctt ttcgttcggc aaatcgccgt
     3541 cgcgcggaac cgcaatcgtt gctaccgtgc tggtgtaaac gaatttagta acccctgcac
     3601 tgcgagcggc gtcgagcagg ttccgcgtcc cggccacatt cgattcgtga atttccgtgg
     3661 gatctttggc ccagaggcgg taatccgcag cgacgtgaaa aacccgctgc acgccttcca
     3721 tggcgcggtc aagagaggcg cgatctcgca agtctccctg cactcgttcg gcgttcaatc
     3781 cttcgagcgc gcgcagatcg ctcgatgcgc gcaccagtac ccggatttct tccccgcgct
     3841 caactaatgc gcgggcgacg tggcttccta gaaagcctgt cgctccggtg accagcgttt
     3901 tcatagaact cgcagtcgtc cgtctgaaga caccaaatcc gaattcgtcg ggggtggtgg
     3961 catcatcgtg ctgcctcctc cggaatgccc gttcgtcggc ggggttttgg acgcgttggt
     4021 atcaggattt ccgcccatgt gtccgcccaa ctgccacttc aacatggtcc aggtatcgcc
     4081 caaattccgc ttcgctccga gcacgggagt cgtttcaaat cccacgtgca tcatgcaatc
     4141 cgcgcagcgc ggatcgtttc ccgggccgta attttcccac ggcgtctggt caatcatttc
     4201 gcggtaagac ccgtaatgat tgtccgtaat tacgtagcat gggcctttcc agccgcgcgg
     4261 attgtaagtc ggcgtgcccc aagctgtaca ttccagctcg cgctcaccgc gcaggaagtc
     4321 aagataaatg ggtgagacca tcaaattgta tttctcgagc agcgccacag cctcgcggaa
     4381 tttctggcga atatccgcac gcgtcataaa aatgtccgag gtctgcacgg ccgagtatcc
     4441 gtaagcggga gccagcatgt agccgtcgac ccccagagtg tcgagatacc catacaaatc
     4501 cgcaatttcg ttcaggtccg tctctttgta aatggtggtg ttcgagcaaa ccaaaaaacc
     4561 cgcagccttc gcggctttga ttccttcgat agcttcgcgg aacacgccgt cgcgctccac
     4621 gcacaggtcg tgcgttcttt caagaccatc cagatgcacg ttgaagaaaa agctggaagt
     4681 cggcttgaat tcgtgcagcc gcttgcggat gaacatgcca ttcgtgcaaa gaatgatgtg
     4741 acgcccgcga tccagaattt ccttggaaag tctgccgatt tccggataga tcatgggctc
     4801 gccgccgcaa atcgaaacga taggcgctcc gcactcatcg gaagcagcca tgcactgttc
     4861 cacggtcatg atttcattga tcgtggattt atattcgcga atgcgtccgc agcctgtgca
     4921 cgtcaaatta caggcgtgca acggctccag catcatcacc atcggaaatt tcttcgtgcc
     4981 gcgaaagcgt tgcccggcca catatttcgc gagattcgcg gtcatcttaa tgggatatct
     5041 caattcactc tcctaagcaa tttgccggtc gaattcctgc ggacgcactt tcaatccgca
     5101 gaattgttga atcccgctca ccgtgtttcg atatcgcgtg agcgcataca acgggaaata
     5161 attacggtac aaatgatatt tcaaatagaa cacgcaggga aatcctgtcc ccgtaaattc
     5221 gttttcggcc catgaaccat ccgcctgctg ctggtccacc agatgagtga ccgctcgcgc
     5281 gatcgccgga tcgctcactt ccgatgccgc cagcaatccg atcaatcccc atgcggtttg
     5341 cgacgccgtg ctattgcctt tgccctttag cgcagggtca tagtaggaag cgatggattc
     5401 tccaaacccg ccatcggaat tttgcacaga tcgcagccat ccgaccgcgc gctgacaata
     5461 gtcccgcgcg gtaagcgcga cagtttcaag cgcacgtagc actccgctcg ttccgtatac
     5521 gtagttcacg ccccagcggc cgaaccacgg gccttcttcg gtttgatcct tcagcaaaaa
     5581 tcgtacggcg cgctcgatag ccggatgcgt tgcgggccat ccgtaccgtc cgaggcattc
     5641 cacgacgcgt gccgtcacat cggcagtcga tggatcgatc attgcattgt gatcggcaaa
     5701 cgggatgtga cacaggaatt gacgatcgtt gttgtgatcg aacgcgcccc agccgccatc
     5761 tcgattctgc atcgccagga gccacgtcag accagtccgc acagccgctt ccatgcgcgc
     5821 tttgtccgga tagtccacgc gttgcagagc catcagcaca aacgccgtat catccacatc
     5881 ggggtaaaaa tcgttgcgga actcaaacgc ccacccgccc ggcatgaccc caggagtctt
     5941 gatcatccag tcgcccgaac cgcaaatttg gttcttaagc agccaggcag ccgcttccac
     6001 gagtgccggg tgattcggag gcaatccagc ctcttcgaga gccaccatgg cgatagcggt
     6061 gtcccaaaca ggcgacacgc agggcgagac acgaatcgtg tccccctcct caatttcgaa
     6121 ccgggaaaat tcattaattt cacgagcagt cagcgggtca tccggtgaat gccccagcgc
     6181 gaccagcgca aaaattgcgt tcatcatcgc tgggtaaatc gccgccaagc cctcgctgcg
     6241 ctccagatgc tccagcatcc attcccgggc ctgacgaaga gctcgctgcc gcaagggctt
     6301 ccaaggcacg cgctcataaa gcttcaaacc gcgatccagt gccagaaaca ggttgcgcca
     6361 gctgaacagt tccttgtccc aactcagcgc aagcgccttc ttcgacggat ccctgtacaa
     6421 ctcatccaca ataattccac ccggaacgct gaagcgtggc ttatgcgcat aaagaatggt
     6481 cagcggaatc acaattccgc gcgtccagga agacatttcg taaatattta tggcaaacca
     6541 gttcggcagc agcatcaatt ccggcggaat cgccggaacc atatcccagc caaccgcgcc
     6601 aaccaacgcc aaataaagcc ttgtgtaaga gttagccgat tccaggccgc ccagttcgtg
     6661 aattcgtttg gccgctcgcc gcaagttctc gcaatcaggc gcatctcccg caagccgcag
     6721 ggcaacatag gccttgatcg tcgcattcag ttccgagggc cctccaaagt agatattcca
     6781 gccaccatct tccaattgcc gccgccggac gtaatttgcc agtttggcaa ttcgctcagg
     6841 attggcttta ttgatgatct gcaaataaaa aatgtagtcg gattcaagtg tcgtgtccgc
     6901 ttcaagctcc ccgatccaat acccttcgga aacctgcagc gataaaaggt aattcacgcc
     6961 acgatcaatc gtgtcggaca ggcgctccga caaagcggca agaacccccg cagatcggga
     7021 ttcgccggac tggtcgaatt gaacacgagt tcctagtgcc atcgcttatt cccgagcctc
     7081 acaaggtctt tgatgcaatt ctagtggaga aggttcatgc gaatacacta aagcgtagcc
     7141 ctcagtgctt tttaagttgt ggcttgggat acttcggtca aatgctcaga catatcaccc
     7201 tggtccccaa gcaccagtac caggatttcc cccgttccta ttatcttagg tcttgcgatt
     7261 tcggtcaaca gggattgcgc cgcgcgtgcg tttacaggtt agcctcttct tttcaatctg
     7321 taacggagat tactcgtctt ttgcgttcac aatgctttcg agccttttaa ttctctccgc
     7381 cagcgcaggc agacggctta tccatgcaaa ttgctcttta aatttttcca acggtctcgc
     7441 gggtgtcccc caaaggattc ccccgctgcg cacaatcttc cctggcagaa tacccgcctg
     7501 cgcacctatt accgctccat ctttaatttc gcagtgatcg gccatcccga tttggccccc
     7561 taaagtgacg tttttcccaa taattactga cccggagatc ccagtttgtg ccgcaatgac
     7621 tgtgttttct ccaatactta cgttatgcgc gatgtgaata aggttatcca gtttcactcc
     7681 ggaacctata accgtccggt ccagcgaccc acgatcaata gtcgtattcg cacctatttc
     7741 cacgtcattg ctgatttcca cttcgccaac ctgcggaaat tttcggtgct tgccgccctc
     7801 ggcaacatat ccgaacccat ccgatccgat tacggtgccg gaatgaagaa ttacgcgatt
     7861 tgcaatgcgt acgcctgagt acagagtcac gcgggggtaa aaacgacacg attcaccaat
     7921 cgtcgaccca cggccaataa agcaaaaagc gccaatttcc gtgcctgagc cgatttgaac
     7981 gtcctcctca atcaccgcgt aagggcccac ggagactcct gccgcaattt gggcggacga
     8041 tgcaatgatc gcagtacgat gtatttcttt tgctatcgga gtggcaggaa taagccactc
     8101 cgcggcacga gcgaatgcca acttcggttt cggtgcgcgc agcaaaacct ttcctgagag
     8161 ggttagtccc ggggcaatca cgacgcacat ggatgccgac gcagcggcac ggtccaggtg
     8221 gcgcggcgat tctacataga tcaggtcggc ggcgcgagcg gacacggtcg atgccacgcc
     8281 agacacacga accgctccat caccctcgag agggcagcca aggaaatccg ccagttcctt
     8341 cattgtgcgt gccatgtaaa gtttctccct gctgaaattt gcttatgaag cgaaaatatt
     8401 ggaactttga ccttccgaac gggagatatg tttatttata cacggatggc tccccagtgg
     8461 ggtgtgtttt ccatcgacga tgcacccaca gccactggtc tggatacttg cgtacataat
     8521 tctcaatcac acgcgtaaac cgctgcgtat tttcgcgaat atccgtggcg tcatcacctg
     8581 tgcgtgtcaa ctccacttcg ggctcaaaac atagcctgta tttgcgggtt gcaccgtccc
     8641 aatgaatgaa tcccggcacc actgcggcgt ctgtgtgcag ggcaaaccga gcaatgccgg
     8701 ctgttgtaca tgcgggaata ccaaaaaaat ccacaaacac gccttccgtc gtttgcgtat
     8761 tttggtcggc gagaattccc actgttcctt tcgcagccaa aacttttaag accgcgcgcg
     8821 cggattgact tttgtcgatt ggttgatttc cgtgcgagca ccggtatttc gtaatcagtt
     8881 cgtccacgaa cgggttatca atggggcgaa ccagaaagtg cagtggatta ccatagaggg
     8941 cctgcgcgaa gggtgccatc tcccaagcgc tcatgtggcc ggtgaggaac aagactccct
     9001 tgccgcgcgc ttgagccgat gcaaaatttt caaagccttc aatgagcacc gtgcgctcaa
     9061 tattattttt tgtgtagtgc ggaaaatgcg caaattccgc gcccatccaa ccgagttgcc
     9121 tcaccatgcc gcgaatcgct gcctgccgct gctttttgtt ccacgttgga aaagccagct
     9181 gcaaattttc cgtagctgcg gcacgcagtc cgggacgcag taaaaataaa aatgccgcag
     9241 ttcgcgcgcc tacaaatctc gcgagtggtc ttggcaaggt tccaatgatc ttgagcagca
     9301 gccataacgc tgcatattcc aggcgttgtt gcacgctggg attataacgg ctgcgcggaa
     9361 agatggtgac tcgtgattcg tgatttgtaa cgcgtgaaac aatcggttcg cgattcggcc
     9421 accgcttcct accaagcact ttcttcctag ccttgcaatc gaagacgaat ttctggaatt
     9481 agtcacgatg ccatttgcgt tttttccacg aattacaaat cacgagtcac tggtcatcgg
     9541 ccacccccct ctcctgctga aatccggtgc cgctgatgct agactcaacc gtttgaattc
     9601 cacgtctcca atccgctcgc gcgccgccga ggaaggcagc ttccaaatcc cactctgggg
     9661 cgttccattt attcttttgt tcgtttatgt atgcatcttc agcggattaa gcgccctcgg
     9721 cgttgtcggc ccggacgagc cgcgctatgc ggcaatcgcc cgggccatgg ccgcaaccgg
     9781 agattggatt acgccgcgcc tatggggttc gccttggttt gaaaaacccg tgctgtatta
     9841 ctggggcgct ggaatcgcca tgcgcatttt tggagtcggc gaattcgccg cgcggttgcc
     9901 gtcggcactg gcagcgctgc ttgcggtgat cgcgattgcc tggaccgctc tgcgctccta
     9961 cggcatcgcc gcgtgctggt tctcgctgtt aatgctgccg acttctgtcg cgatcatcgc
    10021 cttctctcgc gccgcgagcc cggacatgct cttcgcagga ttcctgacgg ccgccatggc
    10081 cgtggcgagc gaaatgttgc agaagccacg tcccggcgcg gtattgcgca tcgcattcgg
    10141 attttttctt ggcgccgctg tgctcgccaa aggccctgcc gccgtaattc tcgcaggcgg
    10201 cgcgacgctt ttttgggccg cactttcccg ccagtggacc gctccatttc gatttctgca
    10261 tccactaatt atcgcggttt tttgcctcac ggccttgccg tggtacgttc tctgcgcgct
    10321 gcgcaatccg gattttctgc gcgtatttat ctggcagcat aatttccagc gctatctgac
    10381 gcccgtgttc gagcaccgcc agccgttctg gttttttggc gtcgtttttc tagtcgcggt
    10441 tcttccgtgg cttccgtttt tgatttcgtc catctcggat gcaaattcaa cgcttcgatc
    10501 gaccgaatgg cgcgattccc ctgcactatt tatcggctcc tggtcgctgt ttacgcttgt
    10561 gttcttcagc ctatcgcaat ccaaattgcc tggttatatt ttgcccggaa ttccgccagc
    10621 tgtgttacta cttgcggcta gcgcggcaag acgacttcaa caaaaatctt ccaccttgtc
    10681 gaatcttgcc atcgctatcg gtttgctctt tccaattggc atagcggttg cccttttaca
    10741 atggaatcat gctccaaaca tagacgcgca gttgcacgcc catatcgcgc gcggatttca
    10801 gattgcgctt ggtgcggccg tcgttctcgc ggcaataatc ataggatatg cattccgccg
    10861 caaaataaac gaagcgattt ttgtcgccgc ctcgaccacg gcattgctta tcctcctcgc
    10921 caatcgcctg gttcttcctg gcacggacac actcgtttct ctgagacccg ccgcaatgtg
    10981 ggccatccaa acgcattccc tgggcgcaga cgatgtggcg gtctacgcac ttccgcgcgc
    11041 ctacggctat ggattgaatt attacttcaa cgcgacgttg ccggaatgga cgccggaaaa
    11101 caccaatgca aagattttat tttgcggccg ggacgcgctc aattccccgc aactggaaag
    11161 cctgcgtggg aaagccaaat tcccggcgac gggcgatgac aaagtttttc ttgtgctttt
    11221 gaattcggat taaatccaca ggaaaaaaac tcttaccgaa tctgtggtaa cgggtctccg
    11281 ctaattggca aattcacaag gaagaaactg acaatacggt tttcgtagtt gcgtttttct
    11341 tcgtccgcca ttccgccata atttccgtgc ggcaacatgg cctgttcatg cggctcagga
    11401 gacatccgaa acaattgcga tgtggaagcg gccaggcgcg ggtcttccgg agattccaga
    11461 taaagttgcg gcacgcctga aagttttccg attcgagtat cgagtggctg cacatttcga
    11521 tattgcgaat tgacccagcc gaaaatcatt tgcgacattc ctgtgacaaa aggtaccgac
    11581 ccaagcccca ggcggcttac ctgcagggcc accatttcat tgggactttt gtaagcggat
    11641 tcggcaacga tggcgcgaac gcgtggatca ttcgtagcct cagccagcgc cacataggcg
    11701 cccatgtttg cgccccacag cccaaagcga ctcacgtcca catctccgcg gttggcaacc
    11761 gcatcgatcg cggcgcgtaa ttgccccact tcctggaggc caagcgtcga atgcccagcg
    11821 gaggacccgt gcgcggaaaa atcaaaagcc agaacgttgt actgttgatc ctgcaagccg
    11881 gaagctaacg ttaggagttc tccccggctc gatccatagg catggcaaag aataattgtg
    11941 ggagctgttt tcagtcctgg gaaaaaccac gcgtctcgcg ttaccccacc gttgacattg
    12001 aaatgcaatt cttcaggatg tcctggaaaa tcttgcagat taatttctga attcctgcgt
    12061 gaaggtgact ccatccggta aaccgcgaac cctgaaatgg cgcccgtaat aagcagtcca
    12121 aaaaatacca ctgccaaaat accgatggcg agtttgctgt accagtacgt cggatgtcga
    12181 aactcggaaa tgaaatccat gcgctcggat tcttgacttc gggcaaactc ccgtccagca
    12241 catcgatccc tggagggggc aatttttctt cagacaatcg catggtaaca caaatggttg
    12301 cgcgtcacgg actagcgatc gaggggttgt ttcttaagga catcgagtct ccaaagggtt
    12361 aaatgtgata ataatgggtg tgtgttcgcc actagccaaa gattaggtgc ccggtccatg
    12421 cagattgtcg acctgcggca gttaacgtcc cgccagcttg agccgttgct cgctgaagaa
    12481 gcacttcaat ggcgcgaaga cttgcgttgg gattatcaca gctcgaccga gctgatcaaa
    12541 aaatttgtcg atgcacgttc gctcaccggc tctgccatcc tggaaaatgg caaaccagtc
    12601 ggatatgcat tttatgtcct ggaagaacac aagggtcttg tcggcgggct atttgtttcc
    12661 cccgcttatt cgcaacagga tctgagccat cagcttttaa ccgacatgct gaacacgctc
    12721 agtggcatcc cgaaactcga gcgcgtggaa gcccaactca tacccttcgg atatcaactt
    12781 gatcccgttc tttccgagta cggtttccgc ctgtacatgc gccaattcat gatcgcgcaa
    12841 ctcgatcaac acacagaagt aacactggac gttcttcccg gtctcattcg cgagggtccc
    12901 ggaatggggc tcattcttga gcattgggat catcgctact ttgagcaatg cgcacggctc
    12961 attcaactgt cctacgccag ccatgtggac ggcgaaatta acgaccagta ccgcagcgaa
    13021 tccggcgcgc tgcgctttct gaaaaacatc atcattcttc ccggctgcgg acagtttcag
    13081 ccagaggcct ctttcatcct gcgcgctcct tacgaaaatc agttggttgc cgtcattctc
    13141 aattcccgcg tcgcaagtgg tgttggccac accactcaaa tctgcgccat gcccggatac
    13201 cagggccgcg gattgggtcg ccgcctcatg gaagcttcgc ttcaagccct ccgtgttcgt
    13261 aatttccaca cggtttcgct gacggtgaca tcgggcaatg aacgcgccgt ccgcctatac
    13321 gaaaggctcg gatttgccac agtcaagaaa tttgccgcag gagtctggac agcattttaa
    13381 ttcctgcatc ccgcgtcaac ggatcgattg aaacgctttg ttatgaataa tttgcgggta
    13441 tgcaggtcgt tgtttttggg cctcaggtaa cgcaacggaa actcccatcc ccctcccgct
    13501 tgccgcctga ttccccgatt gccatactaa aaaaatgcgt cgcacgtcga ttcccgctct
    13561 agccctgctt tccctgctca tccctgcggg cgcattgcgc gcggacgaaa ttcgtctcaa
    13621 agatggaacg aaaattgaag ggacaatcgt aggttttgaa agcgattcat tccgcgtgga
    13681 aacttcatac ggctttgccc tcatccgaaa agacaaagtc gcggatataa atattaccgc
    13741 ttcgaaaaaa gattccgatt caaaaacaaa atcggccgcg tcctttgatt ccactgctcc
    13801 cgctgcgcct ccggtaacac ctgccgttgt gaagcagcct gctacatcca ataattcttc
    13861 gaaggaagga aacggcacgg cgcaggatcg ctccgcatcc tccgcaggag tcacctccag
    13921 tgccgcttca ttcaaggttg atcttgcagc gcccaaagta aagccggtgg ccgtttcgca
    13981 ggtcccgcct ccggccgtga cgcctgccgc atcggccagc gcgtctatgg cgccattgcc
    14041 agccgcgccg tccacaccac cgcccgctcc cgagcctgtc gtgatccgtg acgaaattcg
    14101 cggcaaccag tatgtaaacg tcacctacgg atttcagatg tacaagccgc ccagttggga
    14161 acttattccg ggtgcgcgaa aggcgctgcc cgatgcggtc gccgctctcg gaacaagcga
    14221 tcaaactacg ttgctcgtga ttggccggga acacgccaag gattcactgg acgcgcatgc
    14281 cgcgaaaacc ggcaaagcgc ttagcgacgt gtatgaaaat tatcgccaga tttccagccg
    14341 ccaattatcc gtagccggat ttccagctgt cgaacaacgc gcgcgcggaa gcgccgatgg
    14401 ccgcgattgg tccgtaactc tcttgacgct cgtcaaagga ggcgaagcct atactttgct
    14461 gggaatgacg tgggcggatt ccgacttgat tcaagtgcag gaaaacgtaa tcgcgaaagc
    14521 cgtgaacagt ctgacgtttg ctgcgccgtg atttgtgact cgtgattcgt gattcgtgaa
    14581 gttcagaccg ccgaaattga agcacttccc cgtcccatta aatctcgaat caatccctca
    14641 caaaatttcc gatacgctac aagattcacg aatcacggca tcatcccaca ttcacttcaa
    14701 cggtgtgata ccccgtcgcg ccatccggcc attcgcctga tttcgatgac gtttgcaatt
    14761 tcccgttgcc atccacggcg cgcacttgaa tctcgtgctt cccgctctgc ggatttgccc
    14821 acacgtaccg ccagaaggcc cacacttgtg agggcatcgg gttgctgaat atcgtgcaag
    14881 cattccaggt tgtgccgcca tccgtggaaa tttctacttt ggaaacgcca gcggcattcg
    14941 caatcgccca acctgtcacc acaaaacttt gcccggcaat ctgcgttgag ccatgtggat
    15001 cggtgatcac cgattgcagt tgccgctcgc cttcgttcga ccagcccatc cattcccagt
    15061 atcccaaatg actcttgtcc accagttcga tgcgcgtcag ccatttcggg gttttcatgc
    15121 cgtaaattcc cggcatcagg acacgcagcg gaaacccgtg aatgcgcgtc agcggctcgc
    15181 cgtccatgtc ataggccaga aaatttgcgg gacgagacaa tctttcaatt gtgtgcccgg
    15241 tcgtatagcc ttccgccgcg tacagaatcg cgtacttcgc attcgccttc ggcccggcgc
    15301 gctcgatcag cggatgcagc agggtgccgc gccagttggc cgtgtcgatc aggccgcccg
    15361 caatttcatt cgaaatgcat tccagcgtta gcgtcgtttc gtatggtaag agctttcgaa
    15421 tgtcgtcata atcaaagtgc agcgggtccg tcaccaaccc atcaatcgtc aaggaccact
    15481 tgtgaacgtc caccgcaggc tcgccatgcg acgatgtcac ataaaactga tccacaggcg
    15541 caatcgcccg cagagccgct tccgctccaa atttttcgta gcccttgacg tcaacattgg
    15601 cagccccaaa tccatacgcc agcgaacgat acaccgcata ggggagcagt atccccagcc
    15661 ccgcgccccc agcaatcgaa atgaatctgc gtcggtccat ataggctcgt tcgaatgtac
    15721 cactacttag atgttacgga ggaattccgg attcggatgc atgggactta ctctaaatgc
    15781 tttcagagac cagaagggtg ttaatattga aacgctcaga gcgttcaaaa aagtacactg
    15841 gcttcgcctc gcgctcgctg tacatttgat gccatgatgg tatctctcag ccccttaatt
    15901 atccgagttt atacgtttat tgctataaat ctcccactat taggagcgtt cgcatttgcc
    15961 cttgtaaagt ggcgcggtga tattcggaat tcggatctcc caatgtggcg gaagtggacc
    16021 acaaatatcg gattcgtggc tgtcgcgata cagggaggac tctatttagc ggtttggatt
    16081 tggcctcaac ttaccagagg atctctgcta ggccgatggg cacattgggt gttgccaacg
    16141 tttgcggttg ctgtgccttt cgttcttgct ggaaaaggag cgtctcgttg gtggctcctc
    16201 tcatcatcgg tcatgctatt tgtaatctgc ttctttatag tccttactcc gtgaactcag
    16261 catccagtcc gaacgtaaat tggacatcaa aacttagtca cttccgagaa atggaaattg
    16321 cggaccttca tcgaggggac aaccatgtcg aaggattctt cgccggcggc gcgcacggat
    16381 gggcccaaca gctcgacgtt ggaaagcatt tcgaggacgc tttgattgaa acggaaattt
    16441 cgcaggccgc ctgctagctt gccatcacgc actaaaaacg ttccgtcgcg cgtcattccg
    16501 gttaacactt tttcgtaggg atcgacgtcg cggatatacc agacgcgcgt gaccaggatg
    16561 ccgttatccg tggagcgcac catttcgtcc agtgaggaat tgccgccgct gaaaaccaga
    16621 ttcatcggcg cttcacccat atcgttgggc aagggcaagc cgtgtcccgt tggcttggct
    16681 ttcatttttt tagcggtggc gcgagagtag acaaggtcgc tgggcacgcc cttgtccacc
    16741 agcaagattt tctgacgtgg aattccttct ccatcgaatg gcgcgccgga ttgcagcgga
    16801 tgataggcat catcgtggag cgaaatattt tcgcccagga tgcgtttgcc catgcgcttc
    16861 gtgaaacacg aacgctggtc ccacatcgct gtgcccgtaa aatcgtaaaa taaaaaaccg
    16921 acgagatcga gtgcggccga aggttcgagg atcacggtcc agcgcccggg cgcgacttcg
    16981 gatggcttgc gcgaggcggc ggatttttcg ctggcgttgc gggcgagcgc cacaggatcg
    17041 atttgcgcga gatcgggcga attggatttg gcccagccgg aagaatccga ttctagaatg
    17101 gtaattgaaa attcggcgcg cgtctggcgg tgagaagcga aaaggccgcg cgtgttggca
    17161 atcgcgaatt gcgtgactcc ggtcgagaaa attccagctg ccgtttgctt atttttctcg
    17221 gccatttccg tcgccttgac gacggcgcgt gcgcggtcgg cgggcgtggc gtaggccgta
    17281 ttttcaaaat agcgcgcgac cttctgatat ttttggggac caggcatcgg cagcaagtcg
    17341 ggattccgcg gctggctgcg cgccagcgtc ttcgcggttt ccaccacccg ccgcagcgaa
    17401 tcgtcctctg ttttattggt agtggcgcgc gcggtacggc cgtcgagcac cgtgcgcacg
    17461 gaaacattta agacctgttc cgcgacattc tgatgaatcg tgttgttcgc gaatcgcgtc
    17521 agcgcgtccg acgtcatgcc gatttcgact tcggtttcgt ccgcgtcgct gaatttgaaa
    17581 attttttcag cgatgcggcg cagctccgca tcggatagca aatgcagacc cgcgaccgct
    17641 acgccggatt tgtggtgcgc gcgtggtcgg tcttttttcg cgtgttttgt cttcttcttg
    17701 gccacaaatc cttcaatcac atgcacaact taaattccaa tacgtaattc taattccata
    17761 cttgccttgt gggtcggagc tttagctccg acatgaaaca tccgcaatat ttgggggctt
    17821 tagcccctga cacgtcaggg cttaaaagcc ctttcttgtt cgccagtatt gtcggacctg
    17881 aagatccgac ccacaaccat aaagtccgac ccacaaaaca aatcccgaga ggctggggtt
    17941 ttcgcgccaa cggcgcggag gatgctagcc cagggcaacg ccctgggttc gcgcccaaca
    18001 aacctcgccc gccctgaagg ggcggaggat cccggcgccc cttcagggcg caccttgatt
    18061 cacttcgtct gcaaactagc atgcgccggc gctttcagcg ccggattttt gccaagcaca
    18121 ggagacaatc gccgaaaacg gatcgcggaa atgaaatagg accgctgatc ctatttttgc
    18181 aacgtctccg tcctgatcgc gcaggggtgc agctcctgcc ccgcctgaag acggcgctac
    18241 acaaaacaac ggctcacgat tttgcgaagg cagcgcccaa ttttatgttg cggaagcgcg
    18301 cgggggcggc gccatggcct gtgcccatgg tttgcatggg ttggcccttg ccgcaattgg
    18361 gggtacccca cagcgtccaa tcttcgcgcg aacaaatcgc atcgcaactg ttccagaatt
    18421 cagtggtgat tccgctgtag ctgggatttt tcagcatgcg ctttttcttg ccgcctttta
    18481 tttcccaggc aatttccgtg gaaaattgaa actggtagcg gcggtcgtcg atggaccacg
    18541 agcgattcgt ctccatgtac acgccatcgt ccgtgtcggc gatcaaatca tcgaagcgcc
    18601 atgcgccagg aagaatgctg atgttggtca tgcgaatgat cggcaggcga ttccaggatt
    18661 ccgcgcgcat cgtgccgccg gaggttttct cgccaacgga aatcgcggtc tcgcgattcg
    18721 acaagtagcc ggtgaacagt ccgtcagaaa tgatgggcgt acactgcgcg ggaactcctt
    18781 catcgtcata gccgaatgtt ccgagtccgg gcccgtgctc aagacgcgcg tcggcaacca
    18841 cgttcacgat ttcagaacca tattgcagct tgttcagttt ttcgagcgtc agaaaactca
    18901 tgcctgcaaa attcgcttcc atgcccagca cgcgatcgag ttcgatggga tggccgatcg
    18961 attcatgaat ctgcaaacca agctgcgagc tgtcaagaat taaagtgtgg gcgcctgcgg
    19021 gcgattgcgc ggcggaatgc agcgcgacag cctcttccgc aacgcgtggc gcgttgccca
    19081 ccaaatcgag agactcaata agttcatagc cggagagcat gtgctggccg ccaaaactat
    19141 ttgggtagga ccgtttttga atttcgttgc ccgcgaatgc tgtcgcgcag atgcccgctc
    19201 cagactgcat tttgatctga tgaatggccg agccaatcga ggaagcgaaa aactgctcga
    19261 tgcggcgaag cgtcatcgaa gcttccgaaa ccgtcacgcc ttttatgcgc gacatttcgc
    19321 ggtctgcagc aagcagcaaa tcgagttgcc gctcgatggg aatttgaaac ggatctttta
    19381 cgtagggatt ctgccaagtg tcccgatagg cctcgaccgg aaccatttcc acttccacgt
    19441 cgtgcagctt ggccaaggct gacgctttgg cgatggacac ggcttgcgcg gcgcacgcgg
    19501 caattccctc tcgcgtcagt cggtcggtgg aagcaaatcc ccacgatcct tgcgcgatca
    19561 cgcgaatgcc gataccgaac gattccgatt cggaaagcgt gccaacttgt ccgttttttg
    19621 tggagagatc gcggaggcga atatccatga cgcgggcgtc cgcgtaagtt gcgccgcgga
    19681 ccttcgcggt ttccagcgcc cagttggcga agtcccgcat catggattca cgaaaacaaa
    19741 aacaagagca aaagctttaa cacagagaaa aactaaaaca gagaaaaccc gagggtgctt
    19801 cacgtgcctt cggaccacga tgccagatac agctcctgct ccggcgttag cttgtccatc
    19861 tggacgccca tcgattcgag cttaagcttg gcgatctggc ggtcgatggc ttcgggcacg
    19921 acataaactt caggcttcaa ttccga
//
LOCUS       DASZMB010000063        30477 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4455727, whole genome shotgun sequence.
ACCESSION   DASZMB010000063 DASZMB010000000
VERSION     DASZMB010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 30477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30477
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4455727"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      137..209
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            378..755
                     /gene="crcB"
                     /locus_tag="VG272_02700"
     CDS             378..755
                     /gene="crcB"
                     /locus_tag="VG272_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HWF12618.1"
                     /translation="MTKYWMVGLGGFLGSIARFWLGAYITYRMGARFPFGTFAINISG
                     SFLIGLIVTLLGEHVHWSPNLLYFIPIGFIGAYTTFSTFELEAFRSVRTGDVLMSLLY
                     VVLSVTIGFVAVWLGVLTGKTLA"
     gene            1072..1788
                     /locus_tag="VG272_02705"
     CDS             1072..1788
                     /locus_tag="VG272_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12619.1"
                     /translation="MYRPEKSFSRYPKLAIGVLCLATWGMLGVPAIRSAAAQDPGQAT
                     PPAAAPAAPKPVELPGGDGKPLATEYCQDCHRLTNLTKAHKNLDDWKDTLHTMIDRGA
                     RLPDDKIDPLAQYLAANFGVKADAPPASGSTPAPAAPAAPGGSPAATASATPAPAKAA
                     VVELPEGDGKAIATENCQACHKLTNLTKAHKSLDDWKDTVQTMIDRGANVPADKVDTL
                     VQYLAKNFGPKDAAVPAPSN"
     gene            2022..2096
                     /locus_tag="VG272_02710"
     tRNA            2022..2096
                     /locus_tag="VG272_02710"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2055..2057,aa:Glu,seq:ctc)
     gene            2203..3522
                     /locus_tag="VG272_02715"
     CDS             2203..3522
                     /locus_tag="VG272_02715"
                     /inference="COORDINATES: protein motif:HMM:NF008589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HWF12620.1"
                     /translation="MAISQSSQQNELERQPDLHSLGGEPGRPKKNGWIWILAIIVLAG
                     GAYYFYKSRPSSESKAAPAPGGRAASLGPVSVAVSSALKQNVPYYLSGLGSVTAFNTV
                     TVKSRVDGELQKVYFKEGQFVHEGDLLAEIDPRPFQVALEQMQGQLFRDQAQLNNARV
                     DFKRYDQLAKEGVIASQQVDTQNATVGQLEGAVRADTAQIDNEKLQLVYCKIAAPITG
                     RVGLRLVDQGNMIHSTDSNGLVVITQVQPIAVLFTLPEDNLQEVIQHMKSGELGVEAY
                     SRDDQSKLAGGKLLTVDNQIDPTTGTVRFKAAFANQDLSLWPNQFVNIRLMLATRQNA
                     IVVPLAAIQRGTQGSYVYTVKGGKANMQPVKVDLTQGNIALIASGVAAGDQVVVDGQE
                     RLQQDTPVEVHNASPGGPAGAAPAAGEPGKGSDHAGEGKNKKQGKQS"
     gene            3522..6635
                     /locus_tag="VG272_02720"
     CDS             3522..6635
                     /locus_tag="VG272_02720"
                     /inference="COORDINATES: protein motif:HMM:NF033617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HWF12621.1"
                     /translation="MNPSRPFILRPIATSLLMAAILLAGAVAYRQLPVSALPQVDYPT
                     IQVLTFYPGASPSVMASSVTTPLERQFGQVPGLNQMTSTSSFGASLITLQFVLDLNID
                     VAEQQVQAAINAATTYLPTNLPNPPIYSKTNPADTPVLTLALTSETLPLTKLEDMADT
                     VLSQKIAQLPGVGLVSISGGEKPSVRIQANPTALASYGLSLEDLRTALGQANVNQAKG
                     NLDSSQQAFTIWANDQLMTAESYKPIVITYKNGAPVRIMDVANVIDSAENVKLAAWQN
                     EKPAVIVNIQRQPGANIINVVDRIKNLLPQLKASLPSSVQLSILTDRTTTIRASVSDV
                     QFELMLTVALVVLVIFLFLRSVAATAIPSVAVPLSLVGTFGVMYLLGYSLDNLSLMAL
                     TISTGFVVDDAIVMIENITRYIEEGEPPLQAALKGSEQIGFTILSLTVSLIAVLIPLL
                     FMQDVVGRLFREFAITLSVTILVSAVVSLTLTPMMCSKILRHQKDADKSLFYRASENT
                     LNWIIEQYATILRFVLKHQPATLIVTVGTLALTIFLFLVVPKGFFPVQDTGVLLGISE
                     APATISFSAMAERQQALTKIILQDPDVVSLSSFIGVDGVNMLPNSGRIQINLKSREER
                     ADDGETIIRRLQSKLSDVTGITLYLQPVQDLTVEDRISRTQFQYTMEDADAQELALWV
                     PKLIDKLRELPQLTDVTSDQQERGTQATLQIDRDTASRLGILPQVIDDTLYDAFGQRQ
                     VSTIFTQLNQYHVVLEVDPTFQQNPDALKQIYVHSSNGTQVPLSAFTRYSSELAPIAV
                     NHQGQFPVVTLSFNLKQGASLGQAVDAIKQAQSDLGFPASIHAGFQGTAEAFQAALAN
                     EPILVLAALVTVYIVLGVLYESYIHPITILSTLPSAGVGALLALLLTHTDFSIIALIG
                     IILLIGIVQKNAIMMIDFALEAERKEGKSPIDAIYQACLLRFRPILMTTLAAMLGGLP
                     LALGGGTGAELRRPLGIAIVGGLIFSQALTLFTTPVVYLAFDRLAKRLGHGAPIETAS
                     QANAPAD"
     gene            6727..9840
                     /locus_tag="VG272_02725"
     CDS             6727..9840
                     /locus_tag="VG272_02725"
                     /inference="COORDINATES: protein motif:HMM:NF033617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HWF12622.1"
                     /translation="MHISRPFIERPVATSLLSAAILLAGGVAYLFLPVAPLPKVDFPT
                     LTVQANLPGASPETMASSVATPLERSFGRIAGVTEMTSSSQLGATNITLQLDLDRDIN
                     AASRDVQAAINAAAGQLPTTLPSPPRYRLTNPTDAPIIILSLTSGVIPRSHLYDMADS
                     ILAQKLSQVDGVGLVFVGGGAKPAVRVDLNPLALAHYQIGLAQVRLALQAANANSPKG
                     LLEDGNNRWTVFASDQLLTSEKYQPLIIAYRNGAPVRLSDIANVTEGVEDFRNGAYTN
                     GEPGVVMFIFRQPGANIIDCVDKITALLPELRASLPPMVNFSVTSDRTRTIRASVRNI
                     QFTLMLTVALVVMVIFLFLRNVWATVIPSISVPLSLVGTFGVMYLLGYSIDNLSLMAL
                     AIATGFVVDDAIVVIENVARYLESGETPLQAALKGSKEIGPTVLSMSVSLISVFIPIL
                     LMGGVVGRLFREFAVTLSIAIAVSLCVSLTATPAMCVRFLKSPKDMQHGRAYMLGERI
                     FSRMHRGYATRLRWVLAHQALMLVITLAAISLNVYLYIVIPKGFFPQQDTGRLNGAIV
                     GAQDISFPAMMDKLIQFENIIRTDPAVYSISAFVGGGAENIGRMIIELQDLDQRKVSA
                     DAVIVRLRKKLAVVPGANVYLQAYQDVRVGGLFTSSQYQFTLQGETLEELQTWAPKIE
                     TRLRTLRGLRDVTSDQQDRGLQASLVFDRSTAGRMGIQSQQIDETLYDAFGQRQVSTI
                     YTQLNQYHVVMEVDPQYQNSTDALQKMYVRSSAGTMVPLSAISHYEQRTTALAVNHKG
                     QFPSVTISFNLDPSVALGEAVTEIEAATIQMGVPETIHRSFSGTAQAFQESLRNEPYL
                     ILAALITVYIVLGMLYESYIHPITILSTLPSAGVGALLALRITNTDLSVIALIGIILL
                     IGIVQKNAIMMIDFAIDAERREGKKPVEAIYQACLLRFRPIMMTTTAAMLGGLPLAIE
                     SGMGSELRRPLGISIVGGLMVSQVLTLFTAPVVYLYMERLRSKLARRKTLAPQSVAPS
                     NLSSETP"
     gene            complement(9951..10643)
                     /locus_tag="VG272_02730"
     CDS             complement(9951..10643)
                     /locus_tag="VG272_02730"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWF12623.1"
                     /translation="MSSNDLAILEQEITNCRKCARLVPYCEGVARTKRRAYRDWDYWG
                     RPVPAFGDPEARLVIIGLAPAAHGANRTGRMFTGDRSGDFLYEQLFRAGFANQPDSKR
                     ADDGLILKDVLISAALRCAPPANKPLPEEIRNCLPYLEREIALIRPRAILALGGIALN
                     TYLDLLKRQGLIASRAAFPFSHGASFALPGDLPHLFAAYHPSQQNTQTGRLTPAMFAR
                     VLKQIRKFLESR"
     gene            10989..11384
                     /locus_tag="VG272_02735"
     CDS             10989..11384
                     /locus_tag="VG272_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12624.1"
                     /translation="MKMKFAAATLISFMLLGASVPALARKNPPRPFQLRQEVILNGAQ
                     VPAGIYELTWETEGPTVHVTLRKDGKFVATAEGALVKSGRKYNEDEALLLVNPDGRKS
                     LIEIRIAGSTKAIVLNSSDATVHYSVLKH"
     gene            11844..12641
                     /gene="trpC"
                     /locus_tag="VG272_02740"
     CDS             11844..12641
                     /gene="trpC"
                     /locus_tag="VG272_02740"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HWF12625.1"
                     /translation="MSAETKSGTVLDRIIEARRAAIAHRKKSVPETALRFGVKHSKPV
                     RDFGAALSRDSLNVISELKKASPSRGLIRADFDPVALAKGFEAAGAAALSVLTEEEYF
                     QGDLKYMRDARAAVGLPVLRKDFIVDPWQVWEARATDADSFLLIVAGLDDATLGELLA
                     LGRELGMEALVEVHTREELVRALAVGARIIGVNNRNLRTLEVRVETSMELIKEIPEEC
                     IAVCESGLRTHEDLLRFRAAGFDAFLIGEHLMAQPDPGMALRKLIGT"
     gene            12722..13351
                     /locus_tag="VG272_02745"
     CDS             12722..13351
                     /locus_tag="VG272_02745"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HWF12626.1"
                     /translation="MLTRVKICGITTWEDARLSIDLGASALGFNFYPSSPRAISPADA
                     WSIIRRLPPFVEVVGVFVNWPPLVVDALARAVRLGSVQLHGAESPAEVAELCRKHRVI
                     KAVRVKRGFRSASLGRYRAADAILLDGFAPGLHGGTGRTLDWKLARAARRYGRIILAG
                     GLTPENIAEAVRVAQPYAVDVASGVEARPGRKDPARLRRLFEAIESAQG"
     gene            13475..14686
                     /gene="trpB"
                     /locus_tag="VG272_02750"
     CDS             13475..14686
                     /gene="trpB"
                     /locus_tag="VG272_02750"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HWF12627.1"
                     /translation="MSSATQKWPDAKGRFGPYGGRYVPETLVAPLEKLERAYDDARKD
                     PNFQKELDYLLKNFAGRPTPLQFASRLTEHLGGPRIYIKREDLLHTGAHKINNCIGQG
                     LLAVRMGKRRIIAETGAGQHGVATATVAARLGLECVVYMGVDDMERQRLNVFRMRLLG
                     AEVVGVNSGTRTLKDAINEAMRDWVTNVRTTHYLLGSVLGAHPYPMMVRDFHRVIGVE
                     ARAQILEIEKRLPNLLVACVGGGSNAIGLFHAFLNDASVKMLGVEAGGRGMTLGEHAA
                     RMAAAAGYSGAHPGVLQGTYSYVLQTLDGQIANTHSVSAGLDYPAIGPEHAWLADLHR
                     AEYTAVSDDEAVAAARMLARMEGIIPALESAHALAEIIKRAPKISRDAIVILNLSGRG
                     DKDMEILARYL"
     gene            14795..15628
                     /gene="trpA"
                     /locus_tag="VG272_02755"
     CDS             14795..15628
                     /gene="trpA"
                     /locus_tag="VG272_02755"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HWF12628.1"
                     /translation="MTNQTRIAQRFAVLRRTGEMGLVAYITAGDPTLAATEKFVLALA
                     EAGADVIELGVPFSDPVADGPVIQRASERALRSGTTLAGVLELVKSLRVKTEVPLVLF
                     SYYNPVLQMGLEKFAASAKSVGADGALITDITPEEAGDYRAAMASHGLDTVFLAAPTS
                     TDERLALISKSTSGFLYLISRTGVTGTKDQLADELPALARRVRRFTELPIAIGFGISL
                     PGHVSLLGGLADAAVVGSALVEEIERAESVDAAAAALAARVKLLKGAAAAGMSRRKPQ
                     A"
     gene            15625..15918
                     /gene="pheA"
                     /locus_tag="VG272_02760"
     CDS             15625..15918
                     /gene="pheA"
                     /locus_tag="VG272_02760"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HWF12629.1"
                     /translation="MSVEDWRRKIDEIDKRLVELLNERSRCVVEIGRIKQTTGDALYQ
                     PDREQQVLDGVVSANPGPLPDAAIRRLFERILDEARSVERVVMHGVDGKKENS"
     gene            16018..17037
                     /gene="aroF"
                     /locus_tag="VG272_02765"
     CDS             16018..17037
                     /gene="aroF"
                     /locus_tag="VG272_02765"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWF12630.1"
                     /translation="MVVVMQERAKEEQIQHVINRLVSMGFDIHRSTGASQTVLGAVGV
                     PADFDPRDVEVLDGVSEVVRITHPYKLASRAFRPEGTVIELPLGVRIGGPEVVVAAGP
                     CSVETPEQINLIAQRVAKAGAKILRGGAFKPRSSPYSFQGHGEKGLIMLREAAEKNGL
                     VVCSEVMDASQIQLMLPYVDVFQVGARNMQNYFLLRSLGAVSKPVLLKRGLSATIEEL
                     LLSAEYIMAGGNHNVILCERGIRTFETATRNTFDISAIPVIQKLSHLPIVADPSHGIG
                     IRDKVPPMARAALAAGADGLLVEVHNDPDHAYSDGAQSLYFDQFEKLMAQLRIIAPAI
                     DRTIA"
     gene            17041..17922
                     /locus_tag="VG272_02770"
     CDS             17041..17922
                     /locus_tag="VG272_02770"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HWF12631.1"
                     /translation="MATTFRRAAIVGTGLIGGSFALALRKHFPETVVVGWDKPQVLKQ
                     ALERNAINEGIVDLSVALAGTDLIYVALPVGHTIELLPEIGKFAPRSALVTDACSTKR
                     SVCTAAAEAFREGGAKFLGGHPMAGKEISGIASADANLFRGSKYALIGAETKPGDSKN
                     PDLRVKNFVTLIEKFDAEPVWLAADAHDRAAAVVSHLPQLLSVALAGVVRSQTDEKGL
                     PVTLAGRGLRDALRLAGSPYSVWRDIILTNSDNLEHMLDQMIQALEQVRGELRTRALE
                     EEFSEAGELYRILRDMH"
     gene            17987..18673
                     /locus_tag="VG272_02775"
     CDS             17987..18673
                     /locus_tag="VG272_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HWF12632.1"
                     /translation="MANADVAIEKLFDGAVILSGGFGTCGVPENLIAAVCRKGTKNLT
                     VASNNAGTMDAGLGKLLQTHQIKKMIASYVGENKLFEQQVLSGEVEVELNPQGTLAER
                     MRAAGAGIPAFYTPTGVGTKIAEGKEIREFNGRKYVMERGLRGDFAFVKAWKGDRWGN
                     LIYRMTARNFNPVMATAADYVIAEVEELVELGSLAPASIHTPGIFVNAIFQGVNYEKK
                     IEKRTVRKRA"
     gene            18718..19386
                     /locus_tag="VG272_02780"
     CDS             18718..19386
                     /locus_tag="VG272_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HWF12633.1"
                     /translation="MAQSEESKRELIARRCAQELRDGFYVNLGIGTPTLIANYIPKGM
                     EVMLHSENGMLGVGPFPFEGEEDADLINAGKETVTEIPGTCYFSSSDSFGMIRGGHVN
                     MTVLGALQVDEKGNLANWAIPGKMLKGMGGAMDLVANSPFVIIAMEHATKDGKPKILK
                     QCTLPLTGAEVVDLIVTELAVIAVTPEGLVLREVAPGVTPADVQKLTEPKLIVPENVK
                     TFSL"
     gene            19435..19743
                     /locus_tag="VG272_02785"
     CDS             19435..19743
                     /locus_tag="VG272_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12634.1"
                     /translation="MEPNSIVIVSLHTPKEKLWGCLVSMNASGVTLRCIDLNSFDDFI
                     RQVMDPEGERIGLPTLFFPMQRVERIALDEPHGSIPSLSERFEQRVGRTLRDYLGLFV
                     "
     gene            19765..20325
                     /locus_tag="VG272_02790"
     CDS             19765..20325
                     /locus_tag="VG272_02790"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWF12635.1"
                     /translation="MASRVFTVPNQLTFLRLGFLPFFIISIHYHKYAIALAILILAGL
                     TDGLDGILARSLNQKSALGAYLDPIADKLLLSSSFVVLALNQRIGWWLAALVLGRDVI
                     LLTAACVILVAGGYRPFPPSIYGKATTALQILLVFAVVLLAVKDWIWLQEATKIIVYL
                     VAGFTVFSGFHYCFTVARRIGEQESK"
     gene            complement(20406..21326)
                     /locus_tag="VG272_02795"
     CDS             complement(20406..21326)
                     /locus_tag="VG272_02795"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWF12636.1"
                     /translation="MPLPVRWRYKIDRWRSQIAQMFHSEPKNLRPRLCPACGTLVGST
                     ATRCHQCGASTTYSFAAASRTLSRWMPQTSPVTYAMLAICCVMYALSFVITMKFSGGE
                     GVGGGWMNLGGIANQVSDRLGASLPFSYNLSQPWRFVTAVFLHASLIHIGFNMWVLMD
                     IGPMVEELYGSARYLFLYVVTGAAGYVLSSFMGHFSVGASGSLLGLIGVLLAATTGRK
                     NMAAQALRSALIRWLIYIAVLGFVFSGTDNYAHLGGVASGYLLGRLMPDRAPADMRER
                     QLANFLGWAAGIVVAVSFGFMVMNYLQSAA"
     gene            complement(21582..21815)
                     /locus_tag="VG272_02800"
     CDS             complement(21582..21815)
                     /locus_tag="VG272_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12637.1"
                     /translation="MSKISPFAARRPLKGIPYHDAVPSSMNTPGTNGDVRVGGCEALE
                     VEGAEGGPVGPGAGLGTGCGLGVGAEAGAVETG"
     gene            21733..22299
                     /locus_tag="VG272_02805"
     CDS             21733..22299
                     /locus_tag="VG272_02805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HWF12638.1"
                     /translation="MFIEEGTASWYGIPFNGRRAANGEIFDMNTLVAAHRTLPFGSIL
                     RVTNLNNGRDVEVRVIDRGPFVGNRVLDLARAAAVALDMIGTGTAPVRIELISGSNPT
                     SGDFTVQVGAFADRANAERLRDRLLAQYQPIFIQEYDVPEGHFYRVRVGRVPNPDAAQ
                     QLAARLKNSDGFQTFVMRLDQPNELGIK"
     gene            22362..22703
                     /locus_tag="VG272_02810"
     CDS             22362..22703
                     /locus_tag="VG272_02810"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HWF12639.1"
                     /translation="MDDCLFCKIGEKKIPAKLVYEDEEIFAFEDIQPQAPSHILLCPR
                     KHIVSLTEATAEDAAMLGKLQLVAAKLAGERNLTAGYRTVFNNGRGACQSVFHLHLHL
                     LGGRDFRWPPG"
     gene            complement(22780..23229)
                     /locus_tag="VG272_02815"
     CDS             complement(22780..23229)
                     /locus_tag="VG272_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HWF12640.1"
                     /translation="MEIKNKYGKVIHQGADSYNFDDLILQEAVLEGLILEGAHFDDSN
                     LEGANLRGADLYSGRFFRANLKGADLERAQLQGADFKKANLTNANLRRANLGRDNLGG
                     STQLQGARLEGANLEGTNMEGVEYDAETIFPRAFDPNQNKMILKPSA"
     gene            complement(23280..25724)
                     /locus_tag="VG272_02820"
     CDS             complement(23280..25724)
                     /locus_tag="VG272_02820"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWF12641.1"
                     /translation="MPGNSTGSEPRRALQLPREWIRAWWQRRVPFAISVGLTAFALTV
                     YVYSFIADRPTGGDRPTAVFSFVNRLELNALDLRFRLRPDRYKHPDPRIIIVDIDQRS
                     QEILGRWPFSRTYFANMLDALREDGAKVAAFDITFSKPDETAAPIRELRETIIERQRQ
                     GGTTDPRVIADLDRLSKEYDGDAQFARAIERFGNVVLGNFFLYSEYDLKGVDQKTLDR
                     YANILADFPFPQVRAANPQTGERDLRHLMQGFGDPYGLMPRGAQANIEILSDALRKGH
                     GATGFFNVEPDADGVVRHSLMVLPYGRSKNLDDWDIYGSLDVQAVRLFQGLPDQQMVL
                     DFSETGVTAVEFGPSLILHPDAIGRMMIDYQGDVATYPYVSIADVVRHKFPPGTFKGK
                     IVLVGASATGIGDLRSTPYGGINYPGVEIHANVIDNILNKYLLLRGANQVAVDLLAIF
                     LFGVPLGLWLGLAQPRSMLFGLLLLVPFSFGVWYAFLHGWWLNFIIPSGTLVANVGFV
                     AMYRALVEEKEKRRVRGAFQQYLSPEVIRRLLENPDLVKPRKTEITVMFSDVRGFTTI
                     SEKLDAQELAELLNEYLTDMTQIVFRFDGTLDKYIGDAVMAFWGAPFEDRNHATKSCH
                     AALAMISRLKEMQKKWKADGKPVIDIGVGLSTGSASVGNMGSALRYGYTALGDTVNLS
                     ARIEGLNREYATHILVSDATYAAVEDPLLVFRELDLIRVKGKLKPITLYELVASRGTP
                     DGDAPGLEEHLEFFAQGRACYRERRWQDAQIIFEKLLERWPEDGPARMYLNRCKEYLV
                     AGPEGDWDGVYVMTHK"
     gene            complement(25760..26485)
                     /locus_tag="VG272_02825"
     CDS             complement(25760..26485)
                     /locus_tag="VG272_02825"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWF12642.1"
                     /translation="MSRLVLKFETSLLKEVPLGTRPVTIGRAPDNDIQIDNLAVSNYH
                     ARVYVEAGSLVVEDLNSLNGSFLNDIRVERAMLKDGDAILIGKHEILVDQANDATHSL
                     DAMRRAPVPRVNETMILDTHERRKLLDAAVAAGERSQLSPERLRIPTLIVSRGNTDQQ
                     EYRIASKLTVIGKSKMATVRLRGWFTPEVAAQINKHEDGYYLGRGDRLPKINGVTIDG
                     LTKLNDGDVIDVGRVRLSFMYRD"
     gene            complement(26519..27391)
                     /locus_tag="VG272_02830"
     CDS             complement(26519..27391)
                     /locus_tag="VG272_02830"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HWF12643.1"
                     /translation="MLSRYPFGNTKDHRNPNEALLHIEIGARTDLGRVRKNNEDCYLI
                     DSSLQLFVLSDGMGGEAHGEVASNLAVQTVLTHCRQGENSRTTPIFGESRTDVSDRTN
                     RLASAIHLANRKVFETAASNPEQRGMGATIVAAWIDAQRLSLAHVGDSRAYLLRGGSM
                     DQLTADHSLVAEKVRVGILTPQEADASELQSVLTRAVGTNGSVEVDTDEQALLVGDFL
                     LLCSDGLTRMVTDPEIASTLLTSSTAQESADRLIDLANENGGVDNVTVVVLRVIEKSS
                     GIFDRFKIWKRYSG"
     gene            27745..29583
                     /locus_tag="VG272_02835"
     CDS             27745..29583
                     /locus_tag="VG272_02835"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWF12644.1"
                     /translation="MPESNDLPVVCFADLGANDLAAVTRLARRSPRIRVIGVSRNGSV
                     QDTTGCFATLPRKAKPTVVRRTVDAAIANIELTERERAARADLARAEREMEMLNRIGI
                     ALSQTREVGALLNMILMKAREITGADAGSLYLVEEVHTDALSAGPGERRLRFKLTQND
                     SVQFPFAEIELPLQENSLAGYTALHGNVINLADARQIPRNRPYRFNPRFDEETGYRTR
                     SMLTLPIKNGRGEVIGVLQLINCKQHPEARLTSEEVVEREVLPFSDRAVRLALSLASQ
                     AGVAYENSRLYQDIEKLFEGFVQASVKAIEQRDPTTSGHSLRVSQMTVGLAEIVDRVE
                     TGSYASIRFSPGQMKEIRYAALLHDFGKVGVREEVLIKAKKLYPMQLDVISHRFDYLY
                     MELEAQLEREKVRILMEMDRPEALSRIASLEEKYRRRKLEMESSFEAVLQANEPTVLP
                     EGKFDRLLEIGRQTYHDPHGVEKPILTAEEVRYLSIPKGSLSADERAQIESHVIHSFN
                     FLLQIPWTKQIREIPRIVRAHHEKLNGTGYPYKLRSNEIPVQAKIMTICDIFDALSAS
                     DRPYKKAVAPERALGILEMSVKDQELDPELYKLFLDAGIFRLADNV"
     gene            29719..30288
                     /locus_tag="VG272_02840"
     CDS             29719..30288
                     /locus_tag="VG272_02840"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWF12645.1"
                     /translation="MAKNNVEAPDWSTIPPPVDDGAASHLIGMSVASVSLPATDGSII
                     DLSTLQGRTVVYAYPRTGRPGIENPEGWDQIPGARGCTPQTCSFRDHFAEIKSLGVDF
                     IFGLSTQNTAYQREAAERLHLPFPILSDEKLVLASSMRLPVFETSGMKLLKRLTLVID
                     NGVIEHVFYPVFPPDQDAANVIGWLKTKS"
     gene            30267..>30477
                     /locus_tag="VG272_02845"
     CDS             30267..>30477
                     /locus_tag="VG272_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12646.1"
                     /translation="MAENEKLISWNKIKKRGFEISRFSSCRDRIPDMLHPFISNKKLA
                     RISLLISMLSCCAVRAAAQQPTKPHA"
BASE COUNT         6911 a         8204 c         8388 g         6974 t
ORIGIN      
        1 tcggatttac cgccggatca taccgacaaa acagccgccg gcactaaaac aagagtcaat
       61 cgctgttcat caatgatgta attcaaccgg aacattccga aagcacgcgg ttgacttcca
      121 ttacttaaac cgtatgattg ccaaaggcga tggagttcgc cttataaccg ctgtccttta
      181 gccggacaga tgatgactcc tgacaagttc cttgtcgcgg agtcatgtcc cggcgatgaa
      241 ccagtcaatc aagtcagcat tcatgaagcg caggatgtgc cgatattttt ctcgttgcat
      301 atttgcgcgt ttaaacttct tgcaatttcc gggaagtccg tgaggatgcc gcaaagaaaa
      361 gtctggcgag gacgtggatg acaaaatact ggatggtggg gctgggggga ttcctcgggt
      421 cgattgcacg attctggctg ggggcctaca tcacatatcg aatgggcgcc cgctttccat
      481 tcggcacatt tgccatcaac atcagcggat cttttttgat tggattgatc gtaacgcttc
      541 tcggtgagca cgtacactgg agtcccaacc tgctctattt cattcccatt ggttttattg
      601 gggcctatac aacgttttct acatttgagc tggaagcatt tcgcagtgtt cggacgggcg
      661 acgtcttgat gtctctccta tacgttgtcc tcagcgtgac gataggtttt gtggcagtgt
      721 ggttgggcgt gttgaccggc aagacgttgg cctaaaaaat gaaggaaagc aaaatgccct
      781 ctctgtgcgg aataatgctg cacagagatg tttctacaaa accgcaatgg cttgccgttg
      841 cttttgcaga ggccgccctt cttttcgggc gggcagcttt tttgcctcaa ctcctcaaaa
      901 aaaataatgt cctgccattg gctagcctcg cttgattctt ttcttcgttc atatcaaaat
      961 gaaattctac aggctggagc tacagggaac ttagtttaag tgagtctccg gatggttgaa
     1021 tcgacggaag aatttactgc ctgcacggga atcccgggag gatgtgaaag agtgtacaga
     1081 ccagaaaaaa gtttttcacg atatccgaaa ctcgctatag gcgttctttg ccttgctacg
     1141 tggggaatgc ttggggtacc cgcaatacgc tcggctgccg ctcaggatcc aggacaagca
     1201 acacctccgg cggctgcgcc tgccgcacca aagccggtgg aactgcctgg aggcgacggc
     1261 aagccactgg cgacggaata ttgccaggat tgccacagac taacaaattt aacgaaggcg
     1321 cacaaaaatc tggacgattg gaaagacacg ctccacacca tgattgaccg cggagcacgc
     1381 ctcccggacg acaaaattga tccgcttgca cagtatttgg ccgcaaactt tggtgtcaag
     1441 gctgatgcgc ctcccgcaag cggttcgact cctgctccgg cggcccctgc cgctcctgga
     1501 ggctcaccgg ctgctacggc ctcagctacg ccagcacccg caaaggcagc agttgtagaa
     1561 ttgcccgagg gtgatgggaa agcaatcgca acggaaaatt gccaggcgtg ccacaagctt
     1621 accaatctca ccaaggcgca caaaagtctt gacgactgga aagataccgt tcaaacgatg
     1681 atagaccgcg gcgcaaatgt gccggcggat aaggtcgata ccctggttca ataccttgcc
     1741 aagaactttg gtcccaagga cgcggcggta ccagcacctt cgaattaggg gcaatcttcc
     1801 agcggaagcc acggcctttc tgctcgtggc catctctgaa ttttaacgaa tataaactga
     1861 aggtcaggac cggtgacgct tgcccatggg caggtttgtt tttaggcttg gcttgcgcct
     1921 gcggggtgtc cgtgcgaaaa tcaccactca atcgtccgcg taatcggctg tggtcaacat
     1981 gcctattcaa gtatgtgggg cgaaagcgat tggctcgcga cggcgatatc gtctaagggt
     2041 taggacgtgg ccctctcaag gccaaaatcc gggttcgaat cccggtatcg ctaccaactt
     2101 aagaattctt gagcgattcg ggcggttatg taaccgaatc tgacctcaat acgtcaatca
     2161 gtgcggggtc ggaccactcc ataacaaact gacaggacaa atatggctat atctcaatcc
     2221 tcgcaacaaa atgaattgga gcgccagccg gatttgcact cgctgggcgg cgaacctggc
     2281 cgcccgaaaa agaacggctg gatttggatc ctggcgatca ttgtcttggc tggaggcgcc
     2341 tactatttct ataaatcgcg cccatcttcc gagagcaagg ccgcacctgc gccgggtggc
     2401 cgagccgctt ctctaggtcc cgtgtccgtt gccgtctcct ctgcgcttaa gcaaaatgtt
     2461 ccctactatc tttcgggtct gggatcagtg acagcattta atacggttac tgtaaagagc
     2521 cgtgtcgatg gtgaattgca aaaagtttat tttaaggaag gtcagttcgt gcacgaaggt
     2581 gacctgctgg cggagatcga tccccgtcca tttcaggtgg cgctcgaaca gatgcaaggg
     2641 cagctgttcc gcgatcaagc gcagctgaat aacgcgcgcg tggatttcaa acgctacgac
     2701 caattagcga aagaaggcgt cattgcgtcg cagcaagtgg atacgcagaa cgcaacggta
     2761 gggcagctcg aaggcgcggt tcgcgcggat acggcgcaaa ttgacaatga aaaattgcag
     2821 ctggtttatt gcaagattgc ggcgccgatt accgggagag tgggtctaag actagttgac
     2881 cagggcaaca tgattcactc cacggactca aacggattag tcgttatcac acaagtccag
     2941 cccatcgcag tgctgttcac gttgcctgaa gacaatcttc aggaagttat ccagcacatg
     3001 aagagtggag aactgggcgt cgaagcctac agccgcgacg atcagagcaa actcgcaggc
     3061 ggcaaacttc tgaccgtcga caaccagatc gatcccacta ccggcaccgt tcgattcaag
     3121 gcggcgtttg ccaatcagga tttgtccctc tggcccaacc agtttgtaaa tattcgtctg
     3181 atgctggcga cgcggcagaa cgctatcgtc gtgccgctgg ctgcaattca gcgtggtacg
     3241 cagggatcct atgtgtacac ggtgaagggc ggcaaggcga acatgcagcc cgtgaaagtg
     3301 gatttgacgc agggaaatat tgcgttgatc gcctctggtg tggcggcagg cgaccaggtg
     3361 gttgtagacg gacaggaaag attgcagcag gacactccgg tcgaagtgca taacgcttct
     3421 cccggtggtc ccgccggcgc ggcacctgct gctggggaac ctggcaaggg ttcggatcac
     3481 gcaggcgaag ggaaaaataa aaaacaggga aagcagagct aatgaatccg tcacggccat
     3541 ttattctgcg cccaatcgcg acttccttgc ttatggccgc gattttactt gctggggccg
     3601 tggcgtaccg gcaacttcct gtttccgcac tcccgcaagt tgactatccg acgattcaag
     3661 tcctgacgtt ctatcccggc gcgagtccct cggtaatggc ttcgtcggtg actacgccgc
     3721 ttgagcgcca attcggccag gttcccgggc tgaatcagat gacgtccacc agttctttcg
     3781 gggcgtcgct cattacgctg cagttcgtac tcgatctgaa tatcgatgtg gcggaacagc
     3841 aggtgcaggc cgcgatcaat gccgccacta cctaccttcc gactaatctt ccgaacccgc
     3901 caatttacag caagacaaat ccggcagaca cccccgtgct cactttggcg ctgacttccg
     3961 aaactctgcc actgacgaag cttgaggaca tggcggacac tgtcctgtcg cagaagatcg
     4021 cccagttgcc aggggttggt ttggtaagca tcagcggcgg cgaaaagcct tccgtgcgta
     4081 ttcaggcgaa tcccacggcg ttggcgtcgt atggtttgag cctggaagat ttgcggacgg
     4141 cactggggca ggcgaacgtt aaccaggcca aaggaaatct ggacagttcc cagcaggcat
     4201 tcacgatttg ggcgaacgac cagttgatga ccgctgagtc gtataagccc attgtcatta
     4261 cgtataagaa cggcgcgccc gtccgcatca tggatgtggc gaacgtgatt gacagcgcgg
     4321 aaaatgtgaa actcgcggcg tggcaaaatg aaaagcccgc ggtgatcgtt aacattcaaa
     4381 ggcagccggg tgccaacatc atcaacgtcg tggaccggat taaaaatctg ctgccgcagt
     4441 tgaaagcgtc gcttccttcc tcggtgcaac tttcgatttt gaccgaccgt acgactacga
     4501 ttcgcgcttc cgtttcggac gtgcaatttg aattgatgct gacggttgcg ctggttgtac
     4561 tcgttatttt cttgttcctg cgcagcgtgg cggccactgc cattcccagc gtggccgtgc
     4621 ctttatcgct cgtgggcacg tttggcgtga tgtatttgct tggctacagc ctggacaact
     4681 tatcgctcat ggcgctcacg atttcgaccg gattcgtggt cgatgacgcg atcgtcatga
     4741 tcgaaaatat tactcgctac attgaagaag gcgagccgcc gctacaggca gctcttaaag
     4801 gttccgagca aatcggattt acgattcttt cgctcaccgt ttcgctgatc gccgtgctga
     4861 ttccgcttct gtttatgcag gacgttgtgg gcaggctgtt ccgcgaattc gcaatcacgc
     4921 tgagcgtgac gattctggtt tcggccgtgg tgtcattgac acttacaccg atgatgtgct
     4981 cgaaaatttt gcgccatcaa aaagatgccg ataagagttt gttctacaga gcttcggaaa
     5041 acacgctgaa ctggatcatc gagcaatatg caacgatcct gcggtttgtg ttgaagcatc
     5101 aaccggccac gttgattgtg actgttggta ccctggctct tacgatcttt ttgttcctcg
     5161 ttgtgccgaa gggctttttc ccggtgcagg atacaggagt gctgctgggt atttcggaag
     5221 cgcctgcaac gatttcgttt tccgcaatgg ccgagcggca gcaggcgtta accaaaatta
     5281 ttttgcagga ccccgatgtt gtcagcctct cgtccttcat cggagtggac ggcgtgaaca
     5341 tgctgcccaa tagcgggcgc attcagatca atttgaaatc ccgcgaagag cgagcggatg
     5401 atggagaaac tataattcgc cgcctgcaat cgaagctgag cgatgtgacg gggattacgc
     5461 tttacctgca gcccgtgcag gacctcactg tggaagatcg catcagccgc actcaatttc
     5521 agtacacgat ggaagatgcg gacgcgcagg aacttgccct atgggttcct aagctgatcg
     5581 ataaactgcg agaattgccg caattgacgg acgtaacgag cgaccagcaa gagcgcggca
     5641 cgcaagccac tttgcaaatt gaccgggaca ccgcttcgcg cctcggcatt ctgcctcaag
     5701 tgattgacga tactttgtat gacgcgttcg ggcagcgaca agtctccacg attttcacgc
     5761 agctgaatca atatcacgtt gtgctcgaag tggatcctac atttcagcag aatcccgacg
     5821 cgctgaaaca aatttacgtg cactcgtcga atggaacgca ggttccgctt agcgcattca
     5881 cgcggtattc gtcggaactg gctccgattg ccgtcaatca ccagggacaa tttcctgtgg
     5941 tgactttgtc ttttaacctg aagcaaggcg cttcgctcgg acaagcggtg gatgccatca
     6001 aacaggcaca atcggatctg ggttttccgg ctagcattca cgcgggattc cagggaacag
     6061 cggaagcgtt ccaggctgcg cttgcaaatg agccgatcct cgtcttagcc gctctagtca
     6121 cggtgtacat cgttctcggt gtgctatatg aaagctacat tcatcccatc acgattcttt
     6181 cgacgctgcc ttccgcggga gtaggcgcgc tcctcgcctt gctgctcacg cacacggatt
     6241 tcagcatcat cgcgcttata ggaatcatcc tgctcatagg aatcgtgcag aaaaacgcca
     6301 tcatgatgat cgactttgca ctcgaggcgg agcgcaagga aggcaagagc ccaatcgatg
     6361 ctatttatca ggcttgtctg ctgcgtttcc gtccaattct gatgaccact ctcgctgcca
     6421 tgctgggcgg cttgcccttg gcactcggcg gcggcaccgg agcggaatta cgcaggcctt
     6481 tgggcatcgc gatcgttggc ggattgatct tcagccaggc gcttaccttg ttcacaacgc
     6541 cagttgtgta tctggcattt gaccggctcg caaagcgact cggacacggc gcgccaatag
     6601 aaacggcgtc ccaggccaac gccccagcgg attaaagttg cgcgggcaaa gatttttgca
     6661 ctgttcttgc gaccttctta agagtttgca taacccaggt tttgccaaaa tagagtttaa
     6721 cgattcatgc acatctccag gccttttatc gagcgccccg ttgccacgtc gctgctgagc
     6781 gccgcgatat tattggctgg aggagtggct tatttgtttc tgcctgtggc tcctttgccg
     6841 aaggttgatt tccccacgct tactgtgcag gcaaaccttc cgggcgcaag tccggagacc
     6901 atggcgtcga gcgttgccac gccgctcgag aggtcttttg ggcgcattgc cggtgttacg
     6961 gaaatgacct caagcagcca gctgggcgca acgaacatta cccttcagct ggatctcgat
     7021 cgggatatca atgcagcctc gcgcgacgtg caagccgcaa tcaacgcagc tgccgggcaa
     7081 cttcccacaa cgctgcccag cccgcctcgt taccggctga cgaatccaac ggacgctccg
     7141 attatcattc tttctctgac atccggagtc atcccgcgca gccatctgta cgatatggcg
     7201 gattccatcc tggcccagaa actttcgcag gtggatggag taggtcttgt atttgttggc
     7261 ggcggcgcga aacccgccgt gcgggtggat ttgaatccgt tggcgctcgc gcattatcag
     7321 atcggtctcg cacaagtgcg tctggcgcta caggcggcca acgccaattc gcccaagggc
     7381 ctactcgaag atgggaacaa ccgctggact gtcttcgcgt cagaccagct gctgacgtct
     7441 gaaaaatacc agccgctgat catcgcttac cgcaatggcg cgcctgttcg attgagcgat
     7501 atcgcgaatg tcacggaggg cgtcgaagat tttcgcaatg gggcgtacac gaatggcgaa
     7561 ccgggtgtcg tcatgtttat cttccgccag cccggcgcga acatcatcga ttgcgtcgac
     7621 aaaattaccg cgcttcttcc tgaattgcgc gcgtctctgc cgccgatggt gaatttttca
     7681 gtcacttcgg acaggacgcg aacaatccgc gcatcggtcc gcaacatcca gttcacgctg
     7741 atgctcacgg tggcgctggt ggtgatggtc atattcctgt tcctgcgcaa cgtctgggct
     7801 acggtaattc ccagcatttc cgttccgctt tcgctcgtgg gcactttcgg agtgatgtat
     7861 cttctgggat acagcatcga caatctttcg ctcatggccc tggcgattgc cactgggttc
     7921 gttgtagatg acgcgattgt agtgatcgaa aacgtggcgc gctatctgga gtcgggcgaa
     7981 acaccgctgc aggcggccct caaaggttca aaagaaatcg gaccgaccgt tttgtcaatg
     8041 agcgtctcgc tgatttccgt cttcattcca attttgctga tgggcggagt tgtggggcgg
     8101 ttgttccgtg aatttgcggt aacgctcagc attgcaattg ccgtttcgtt gtgcgtatcg
     8161 ctcacggcga cgcctgcaat gtgcgtgcga tttctgaaat ctccgaaaga tatgcagcac
     8221 ggccgcgcgt acatgctggg cgaaagaatt ttttcgcgca tgcatcgcgg gtatgccaca
     8281 cgtttgcgct gggttctggc gcatcaggcg ctcatgttgg tgataacgct cgcggccatc
     8341 agccttaatg tctatttgta tatcgtcatt cccaaaggtt tttttccgca gcaggatacg
     8401 ggccgcttga acggcgcgat tgttggagcg caggacattt catttccggc gatgatggac
     8461 aagctcatcc agtttgaaaa tattattcgg actgatccag cggtgtacag catctcggca
     8521 ttcgttggcg gcggggcgga gaatattggc cggatgatta ttgagttaca ggatcttgat
     8581 cagcgaaaag tcagtgctga cgcggtcatt gtccggttgc gaaagaaact cgccgtagtt
     8641 cccggcgcca acgtctattt gcaggcatat caggacgtgc gcgtaggcgg cctgtttaca
     8701 agctctcaat atcaattcac gctgcaggga gaaacgctcg aagagctcca gacctgggct
     8761 ccgaagatcg agacaaggct cagaactttg cgcggattgc gtgacgtcac cagcgatcag
     8821 caagacagag gattgcaggc ttcgctcgtg tttgaccgca gcaccgccgg gcgcatgggc
     8881 atccaatcgc agcaaatcga cgaaacgctt tacgatgcgt ttggccagcg ccaggtctcc
     8941 acaatctaca cgcagctgaa tcaatatcac gtggtgatgg aagtcgatcc ccaatatcag
     9001 aacagcacag atgcgcttca aaagatgtat gtgcgttcgt ccgcgggaac catggttccg
     9061 ctcagcgcga tttcacacta cgagcaaaga accaccgcgc tagcggttaa tcacaaggga
     9121 cagtttccgt ccgtcacgat ttctttcaat ctcgacccga gcgtcgcgct cggcgaagcc
     9181 gtcacggaaa ttgaagccgc cacgattcag atgggagtgc cggaaaccat tcaccgcagt
     9241 ttttcgggca cagcgcaggc atttcaggaa tcattgcgca acgaacccta cctgattctg
     9301 gccgcgctca tcactgtgta tatcgttttg ggcatgctgt atgagagcta cattcatccc
     9361 atcacaattc tctccacgct gccatcggcg ggagtcggcg ctttgcttgc tttacggatt
     9421 acaaacacgg atttgagcgt catcgctttg atcggaatta tcctcctgat tggaatcgtg
     9481 cagaaaaacg caattatgat gatcgatttc gcgatcgatg cggaacgcag agaaggaaag
     9541 aagccggttg aggccatcta ccaggcatgc ctcttgcgct tccgtcccat catgatgacg
     9601 acgacagcgg ccatgctcgg gggccttcca ttggccattg aatcagggat gggttctgag
     9661 ctgcgacgtc cgttgggaat cagcattgtg ggcggcctga tggtcagcca ggtgcttaca
     9721 ttgttcactg cgcccgtcgt ttacctttac atggaacgcc tgcgaagcaa gctcgcccgc
     9781 cgcaaaactc tggcgccgca atcggtagcg ccctctaacc tatccagcga aacgccatag
     9841 tgaatcgttg gcggatttgc tttttgtggc gccggcgcta caaaggccgc ttcactccga
     9901 aaacgaatac gttattttcc acagaataaa attaaaacaa gccaagccat ttaccgactc
     9961 tccaggaatt tgcgaatttg ctttagcact cgcgcgaaca tcgccggtgt cagcctccca
    10021 gtctgcgtat tctgttggct ggggtgatac gccgcaaaca ggtggggtag atcaccaggc
    10081 aaggcgaagc tcgcgccatg cgagaaagga aatgcggcgc gcgacgcgat caatccttgc
    10141 cgcttcagca aatcgagata ggtgttgagc gcgatgcccc ccaacgcaag aatcgctcgc
    10201 gggcgaatga gcgcaatctc gcgttcgaga tacggcaaac agttgcggat ttcttccggc
    10261 agcggtttgt tggcaggcgg cgcgcaacgt aatgccgctg aaatcagcac atctttcagg
    10321 attagcccat cgtcggctcg tttcgagtct ggttgattcg caaaacctgc tcgaaagagt
    10381 tgttcgtaga gaaaatcgcc ggaacggtcc cctgtaaaca ttctgccggt gcggttggct
    10441 ccatgcgctg ccggcgcgag gccgataatg acgaggcgcg cttcaggatc gccaaatgcg
    10501 ggaacgggcc gtccccaata gtcccaatcg cgataggcac gtcttttcgt acgcgcgaca
    10561 ccctcgcaat acggcaccag ccgggcacac ttccggcaat tcgtgatctc ctgctccaaa
    10621 atggctagat cgttgctcga cacgacacct ccaagaaaat aaatacgctt tttctaaagt
    10681 tctttcttga aattataggc tttttctata ttctgctttc cattcactga atagcaatca
    10741 aagtttaatc tgaacgcaag attcgaatag cagactgcaa cgaatccgga tacgggaatg
    10801 gaggctacct ggctgggatc cattcgaggt gtctctcccg gctcccaaat tggttgcaaa
    10861 ccaatttggg tcaccatcca ttttggacgc caaccaattt gagtctggtc tcgattctgc
    10921 cgataggcag aatcgaaaca ttcaacgcgt ctcccacgga agaaaatcct gacggaggcc
    10981 agaaccgagt gaagatgaaa ttcgccgctg ctactcttat tagtttcatg ctgctgggcg
    11041 caagcgtacc ggcattggca aggaaaaatc cgccgcggcc ctttcagctg cgccaagagg
    11101 ttatcctcaa tggggcacaa gttccggcgg ggatatatga attgacgtgg gaaactgaag
    11161 ggccgacggt gcatgtaacg cttcgaaagg acgggaaatt cgtagccacc gccgagggcg
    11221 ctttggtgaa gagcggcagg aaatacaacg aagatgaagc gctgctgctc gtaaaccctg
    11281 atgggcgcaa gtctctcatc gagattcgca tcgctggatc aacgaaggca attgttctga
    11341 acagtagcga tgccaccgtc cattattccg ttttgaaaca ctaaactttt gctcggtcga
    11401 agggttaatg cgccatttgc ataacctgca acacagtcac gctggatgaa cttcgtgccc
    11461 ggacgaaagc aaatgcctag tccctcaacc tattttgact tgctgataag aaattcagag
    11521 ctttttgggg ccgtctcctc catacggatc tggtagtgtc ctaatttgaa aatttctcga
    11581 atttaggagg gctgggtttc aaatcggcca tttaggttgc ctttcgtcgc tggcttcggc
    11641 cccccgaagt ttcaggggtt aaaagccccc atcaaaccaa gcgcgtcgtc gggactggag
    11701 tcccgaacac ctaaggaacc gtgccacgcg aattcttttc aaactagaac agcaccacgg
    11761 atctactgac gttgacttca gcagacctgc cacttagcat agtgtgctcg tctaattcag
    11821 accttcccgg tggacgggaa cctatgtcgg ctgaaaccaa atcgggtact gtgctcgacc
    11881 gcattattga ggcgcgccgc gctgcgattg cccatcggaa gaagtctgtc ccggagacgg
    11941 ctttgcggtt cggggtgaag cactccaagc cggtgcgcga tttcggcgct gcgctgtcac
    12001 gtgactccct gaatgtgatt tccgaactca aaaaggcatc gccttcgcgc ggcttgattc
    12061 gcgcggattt tgatccggta gctctggcca aaggttttga ggcggcgggg gctgcggcgc
    12121 tttctgtgct tacggaagag gaatattttc agggggattt gaaatacatg cgtgacgcgc
    12181 gcgcggcggt gggtttgccc gtgctgcgca aggatttcat tgtcgatccg tggcaggttt
    12241 gggaagcgcg cgcaacggat gccgactctt ttctgctgat tgtggccgga cttgacgatg
    12301 cgactcttgg cgaattgctg gcccttggcc gtgaattggg aatggaggcc ctggtggaag
    12361 ttcacacacg cgaagagctg gtacgcgcgc ttgcagtggg tgcgcgcatt attggcgtga
    12421 acaaccgcaa tctgcgcacg ctcgaagtgc gtgtcgaaac atccatggaa ctgatcaagg
    12481 aaattcctga ggaatgcatc gcagtatgcg aatcgggttt gcgcacgcac gaagatttgc
    12541 tgcgctttcg cgcggctggg tttgacgcgt tcctgattgg cgagcatctc atggcgcagc
    12601 cggacccggg gatggctctt cgcaaattga ttggtactta agtagcgctg gcttcagccc
    12661 gaggcgtttg tccttgcaag ttcaaaaatc cgcaggctga agcccgcgcc acaaaaatct
    12721 catgctgact cgtgttaaaa tttgtgggat caccacttgg gaagacgcgc gcttatcgat
    12781 tgatctggga gcttccgcgc tcgggtttaa tttttatccg tccagtcccc gcgccatcag
    12841 ccccgcggat gcgtggagta tcattcggcg cctgccgccg tttgtcgagg tggttggtgt
    12901 gtttgtgaac tggccgccgc tcgtcgtaga tgcgctggcc cgcgccgtgc ggcttggttc
    12961 ggtgcagctt cacggcgcag aatcgcccgc ggaagtggcc gaattgtgtc gaaaacatcg
    13021 cgtcatcaag gcggttcggg tgaagcgcgg attccgctcc gcttcgcttg gtcgataccg
    13081 cgcggcggat gcgatcctgc tggatggatt tgcgcccggc ttgcatggcg gcactggccg
    13141 cacactggat tggaaattag cccgcgcggc gcgacgctac ggacgaatta ttctcgcggg
    13201 aggcctgacg ccggagaata ttgccgaagc agttcgtgtt gcgcaaccct atgccgtgga
    13261 cgtagcgagc ggcgtggaag cgcgacccgg gcgaaaggat ccagcgcggt tacgacggtt
    13321 attcgaagca atagaatccg cgcagggata agtttgcggc tgctgcagcc cgcctcttca
    13381 gaggcggggg tttagatgtc gcgtcgtcta aatccccgcc tctgaagagg cgggctacag
    13441 tagtcgggtt acagttagct agcagaagga aacaatgagc agcgcaacac aaaaatggcc
    13501 ggatgcgaag ggccgattcg gtccttacgg aggccgctac gtaccagaga cgctcgtcgc
    13561 tccactcgaa aaactcgagc gcgcctatga tgacgcgcgc aaagatccta attttcaaaa
    13621 agaactcgat tacctgctca agaatttcgc tggacgtcct acaccgctgc aatttgcttc
    13681 acgactgacg gagcatctgg gcgggcctcg catttacatc aagcgcgaag acctgctcca
    13741 tacgggcgcg cacaagatta acaattgcat tggacaggga ttgcttgcag tgcgtatggg
    13801 caagcgccgc attatcgcgg aaaccggcgc agggcagcac ggagttgcca cggcgacagt
    13861 tgcggcgcgc ctcggcctcg aatgcgtcgt ttacatgggc gtcgacgata tggagcggca
    13921 gcggctaaac gttttccgca tgcgtttgct gggcgcggaa gtcgtcggcg taaactccgg
    13981 cacgcgcacg ctgaaagatg ccatcaacga agcgatgcgc gactgggtca ccaacgtgcg
    14041 caccacgcat tatttgctgg gctcggtttt gggagcgcat ccgtatccga tgatggtgcg
    14101 cgattttcat cgcgtgattg gcgtggaagc gcgcgcgcag attctggaaa ttgaaaagcg
    14161 tttgcccaat ttgctggtcg cgtgtgttgg cggcggctcc aacgcaatcg gtctgttcca
    14221 tgcatttctg aacgatgcca gcgtcaaaat gcttggcgtt gaagctggcg ggcgcggaat
    14281 gacgcttggc gagcacgcgg ctcgcatggc tgccgcggcg ggatattcag gcgcgcaccc
    14341 aggagttttg cagggaacat attcctacgt cctgcaaacg ctggatggac agatcgccaa
    14401 cacgcactcc gtttccgcgg gactcgacta tccggcgatt ggcccggagc acgcatggct
    14461 cgctgatttg catcgcgctg agtacacggc agtgagcgat gacgaggcgg tggctgccgc
    14521 gcgcatgctg gcgcgaatgg aaggcataat tcccgcgctc gaatcggcgc acgcgcttgc
    14581 cgaaataatc aagcgcgcgc cgaaaatatc tcgcgacgca attgtaattc tgaatttatc
    14641 cgggcgcggc gataaggaca tggaaatcct ggcccgctac ttgtaggttc tagtagcgcg
    14701 ggcttcagcc tgcggagttt tggacttgca agaactaacc cccgcaggct gaagccctcg
    14761 ccacaaaatg cgaatgccgc acgcgaccac aaccatgacg aatcagacgc gcatcgcgca
    14821 acgatttgcc gtcctgcgcc gcacaggtga aatgggcctc gttgcctata ttactgctgg
    14881 cgatcctacg cttgccgcaa ccgagaagtt cgttctcgca ctggctgaag cgggcgcgga
    14941 tgtaatcgaa ctgggtgtgc cgttcagcga tcctgtcgcg gacgggccgg tgattcagcg
    15001 tgcaagcgaa cgcgccttgc gaagcggaac aacgctcgcc ggtgtcctcg aactagtaaa
    15061 atcgctgcgc gtgaagaccg aagtgccgct cgttttgttc agttattaca atccggtttt
    15121 gcaaatgggg cttgaaaaat ttgcggcatc cgcaaaatct gtaggcgccg atggcgcgtt
    15181 gatcacggac atcacgcctg aagaggccgg agattatcgt gctgcgatgg cctcgcacgg
    15241 gttggataca gtttttctgg ctgcgccgac gtccaccgat gagcgcctcg ctcttatttc
    15301 aaaatcgacc agcggatttc tatatttaat ttcgagaacg ggtgtgactg gaacgaaaga
    15361 ccagcttgcg gatgaattgc cggcgcttgc tcgtcgggta cggcgcttca ctgagctgcc
    15421 aatcgcaatc ggcttcggta tttcgttgcc gggacatgtt tctttgttgg gcggtttagc
    15481 ggatgccgcc gtggtcggat cggctttagt ggaagaaatt gagcgcgccg aatcggttga
    15541 tgcggcggcc gcagctctgg ccgcgcgagt caaattgttg aaaggcgcag cggcggctgg
    15601 aatgagccgg cggaagcccc aagcatgagc gtagaagatt ggcgtcgaaa aattgatgaa
    15661 attgacaagc gtttggttga attgctcaac gagcgttcgc gatgtgtcgt tgaaatcggg
    15721 cgaattaaac aaacgacggg cgacgcgctg tatcaaccgg acagggaaca gcaggtgctc
    15781 gacggcgtcg tgagtgcaaa tccaggcccg cttccggatg ccgcgattcg tcgcctattt
    15841 gaacgcattc tggatgaagc acgttccgtg gagcgcgtag tgatgcacgg cgttgacgga
    15901 aagaaggaga attcgtagcg ccggcgtctc gccggtgcta ccagaaccat gcatcgccgt
    15961 agattgagcc tggccgcaat gcacagcaga attatttgaa caggaacagg agttgcaatg
    16021 gttgtcgtaa tgcaagagcg cgcgaaggaa gaacagatcc agcacgtaat caaccgcctc
    16081 gtatcgatgg gattcgacat tcatcgctcg acgggcgcgt cgcaaaccgt gctgggcgct
    16141 gtgggagttc ctgcggattt tgacccgcgc gatgtggaag ttctcgacgg cgttagcgaa
    16201 gtggtgcgca tcacccaccc ttataaactc gccagccgcg catttcgccc cgaaggaact
    16261 gtgattgaat tgccgctcgg agtccggatt ggcggcccgg aagtggtcgt cgcggctgga
    16321 ccgtgttccg tggaaacgcc ggaacagatt aacttaatcg cgcagcgcgt ggcaaaagcg
    16381 ggagcaaaaa ttcttcgtgg aggcgcattt aagccgcgca gctccccgta cagttttcag
    16441 ggacatggtg agaaagggct catcatgctg cgcgaagccg cggaaaaaaa cggtctggtc
    16501 gtttgtagtg aagtgatgga cgcatcgcaa attcaattga tgctgccgta cgtggatgtt
    16561 tttcaggtcg gcgcgcgcaa catgcagaat tattttctgc tccgttctct cggagccgtc
    16621 agcaagcctg tgcttctcaa aagaggattg tccgctacga tcgaagagct tttgctaagc
    16681 gccgaataca tcatggccgg cggtaaccac aacgtgattc tgtgcgagcg aggcattcgc
    16741 acgtttgaaa cggccacacg caacacattc gacatctcgg cgattcccgt cattcagaaa
    16801 ctctcgcatc ttcccattgt tgccgaccct tcgcacggca tcgggattcg cgataaagtg
    16861 ccgccgatgg cgcgcgccgc gcttgctgcc ggagcggatg gtttgctggt cgaagtccac
    16921 aatgatcccg accacgcgta ttctgacggc gcgcaatcgc tctacttcga ccaatttgaa
    16981 aaactcatgg cccaactgcg catcatcgcc cccgccattg accggactat cgcttaagcc
    17041 atggccacca ccttccgccg cgccgcaatc gttggcacag gattaatcgg tggctcgttt
    17101 gcgctcgctc ttcgcaaaca ttttccggaa actgtcgttg tgggctggga caagccgcaa
    17161 gttctgaagc aagcgctgga aagaaacgcg atcaacgaag ggattgtgga tctgtcagtg
    17221 gcgctggccg gaacggattt gatttatgtc gcgctgcccg tgggtcacac aatcgaattg
    17281 ctgccggaaa ttgggaaatt tgcaccgcgg agtgcgctgg tcacggacgc gtgcagcacc
    17341 aagcgctccg tatgcaccgc cgccgcggag gcgtttcgcg aaggtggcgc caaatttctc
    17401 ggcggtcacc cgatggcggg aaaagaaatt tccggaatcg catctgccga cgcgaattta
    17461 tttcgaggat cgaagtacgc gctgatcggc gcagagacga agccagggga ctcaaaaaat
    17521 ccggatttgc gcgtcaaaaa tttcgtcacg ctcatcgaaa aatttgacgc ggagcctgtg
    17581 tggctggcag cggacgcgca tgatcgggcg gcggccgtag tttcgcattt gcctcagctt
    17641 ctttccgtgg cgttggcggg agtcgttcgc agccagacag acgaaaaagg gttgccagtc
    17701 acgttggcgg ggcgtggcct tcgcgatgct ttgcggctgg cggggagccc atattccgtt
    17761 tggcgtgata ttattttaac gaacagcgac aatcttgagc acatgctgga tcaaatgatc
    17821 caggccctgg aacaagttcg cggcgaattg cgcacgcgcg ctctggaaga ggaattctcg
    17881 gaagccggag aactctacag gattctgcgg gatatgcatt aaattatcca aataaatctg
    17941 cggttacttc ttaaattcgt gggggcatga ttcgatggat aaacgcgtgg ctaacgccga
    18001 tgtagcgatt gaaaaacttt tcgacggcgc cgtcatcttg tcgggcggat ttggaacgtg
    18061 cggcgttcct gaaaatctga ttgccgccgt gtgccgcaag ggcacaaaaa atctgacggt
    18121 ggcctcaaac aatgcgggca cgatggatgc cggcctggga aagctgctgc aaactcacca
    18181 gattaaaaaa atgatcgcca gctacgtggg cgaaaataaa ttattcgagc agcaggtgct
    18241 cagcggagaa gtggaagtcg agctcaatcc gcagggcacg ctcgccgagc gcatgcgcgc
    18301 ggcaggcgcg ggcattcccg ctttctatac gcccacggga gtcggcacga aaatcgccga
    18361 aggaaaagaa atccgcgaat ttaacggtcg aaaatatgtg atggagcgcg gcctgcgcgg
    18421 cgatttcgca ttcgtcaagg cgtggaaggg cgaccgctgg ggcaatttaa tttaccgcat
    18481 gacggcgcgg aattttaatc ccgtgatggc gacggccgcg gattacgtaa ttgccgaggt
    18541 cgaagaactg gtcgagttgg gatcgctggc gccggcgtcc atacatacgc ccggaatttt
    18601 cgtgaacgcg attttccagg gcgtcaatta cgaaaagaaa attgaaaaga gaacggtacg
    18661 aaagcgcgcg taacgcggga ttgcatcaca tttgcatgat tcggggaagg gaagaacatg
    18721 gcccagtctg aagagtccaa gcgggaactg attgcacggc gctgtgcgca ggagttgcgc
    18781 gacggctttt atgtaaatct cggcattggc acgccgaccc tgattgccaa ttacattccg
    18841 aaaggaatgg aagtcatgct gcactcagaa aacggaatgc tcggcgtcgg cccatttccc
    18901 tttgagggcg aggaagacgc ggatttgatc aacgccggca aggaaacggt cacggagatt
    18961 cccgggacat gctatttttc tagctccgac tcgtttggaa tgattcgcgg cgggcatgtg
    19021 aacatgacgg tgctcggcgc gttgcaagtg gatgaaaaag gcaatctcgc gaactgggcg
    19081 attcccggaa aaatgctcaa gggcatgggc ggcgcgatgg acctggttgc caattctccc
    19141 ttcgtgatta tcgccatgga gcatgcgacc aaggacggca agcccaagat tctcaagcag
    19201 tgtacgctgc cgttgaccgg cgcggaagtt gtcgacctga tcgtcacgga acttgcggtc
    19261 atcgcagtca ctccggaggg tttggtgctg cgcgaagtgg cgccgggagt aacgcctgcg
    19321 gacgtacaga aactgacgga gccgaaatta attgtgcctg aaaacgtaaa aacattttca
    19381 ctgtaacgga agcgaaacca ttgctccgcc tgcgggggcc aaacgagtgc atccttggaa
    19441 cccaactcca tcgttatcgt cagcttgcac acgcccaagg aaaaattatg gggctgcctt
    19501 gtttccatga atgcttcggg agttactcta cgctgcattg atttaaattc gtttgacgat
    19561 tttatcaggc aggtgatgga tcccgaggga gagcgcatcg gacttcccac attgtttttc
    19621 ccgatgcagc gcgtcgagcg cattgcgctg gacgagccgc acggatcgat cccttcgctt
    19681 tctgagcggt ttgagcagcg cgtggggcgc actttgcgtg attatctcgg tctttttgtg
    19741 tgagcggaat ctaaatttaa aaaaatggcc tcccgcgtct ttaccgttcc taatcaactc
    19801 acgttcttgc ggctgggttt cctgccattc tttattattt ccatccatta ccacaaatat
    19861 gccatcgcgc tggcgatcct catcctggca gggcttacgg atggtctgga cggaattctc
    19921 gcgcgttcac tgaatcaaaa aagcgccttg ggcgcctatc tcgatcccat cgcggataaa
    19981 ttgctgctgt cctcctcttt tgttgtgctg gcgttgaatc aaaggattgg atggtggctc
    20041 gctgcgctgg ttctggggcg ggacgtcatt ctactgacgg cggcatgcgt gattctagtg
    20101 gcgggagggt atcggccatt tccgccgagc atttacggca aggccacgac ggcgctacag
    20161 attttgctcg tatttgcggt ggtgctgctc gcggtaaagg attggatttg gctgcaggaa
    20221 gctacgaaaa ttatcgtgta cctggttgct ggattcacgg ttttctcagg cttccattat
    20281 tgctttacag tagcccgccg cattggggag caggagtcta agtagcacag gcttcagcct
    20341 gtggggggtt tagtccttgc aaagtctaaa ttccacagac tgaagcctgt gctacttaaa
    20401 tccctctacg cagcactctg caaataattc atcaccatga agccgaagct gaccgcgaca
    20461 acaattcccg cggcccatcc cagaaaattt gcaagctgcc gctctctcat gtccgcggga
    20521 gcacgatctg gcatcaggcg gccaagcaaa tagcctgagg ctacgccgcc aaggtgagcg
    20581 taattgtccg tgccgctaaa tacaaaacct agaacggcaa tataaatcag ccagcgaatc
    20641 aatgcgcttc gtaacgcttg agctgccatg tttttcctac cggttgtagc cgcaagtagc
    20701 acgccgataa ggcccaacaa tgaacccgac gcgccgacgc tgaaatgacc catgaacgaa
    20761 ctgagaacat atccagcggc gccagttacc acatacaaaa acaaatagcg cgcggagcca
    20821 tataattcct cgaccatcgg cccaatatcc atcagcaccc acatgttaaa gccgatgtga
    20881 atgaggctgg cgtgaagaaa tacggccgtt acaaatcgcc aaggctgact gagattatag
    20941 gagaatggga gcgacgcgcc caagcgatcg ctgacctgat tggctatgcc gcccagattc
    21001 atccaacccc cgccgacgcc ttctcctccc gaaaatttca tcgtaattac aaaggacagt
    21061 gcatacatca cgcagcagat ggccagcatg gcataggtaa caggcgaagt ttgaggcatc
    21121 cagcgactca gtgtgcggct cgcagcggcg aacgaatacg tcgtgctggc gccgcactga
    21181 tggcaccggg tcgccgtaga gccgacaagt gtaccgcagg ccggacagag tcgcggccgc
    21241 aggtttttcg gttccgaatg aaacatctgc gcaatttggc tgcgccagcg gtctattttg
    21301 tagcgccagc gaacgggaag tggcacgtca tctccttgct cattgctcga aaaatttggc
    21361 acgcctataa tagccggaat cgcgcgcgga cgcgaaatgc ggttacgtaa ttgagattcg
    21421 agtagctcgg aaagtgaaca gggccttgat tgcaagcctt caaagttggc ggtgcctcga
    21481 ttgactgaac gtattcccgc gcgagcagtc ccatttttgc gttccagatg gctggtgctt
    21541 actatcgttg ccgcccttgc ggttgcgggt tgcgcgggcc gtcacccggt ttccactgct
    21601 ccggcttccg cgcctactcc aagaccgcaa ccggttccta gtcccgctcc ggggccaact
    21661 ggaccaccct ccgcgccttc cacctcaagc gcttcgcagc cgccgactcg aacatcgcca
    21721 ttcgttcccg gcgtgttcat cgaagaggga accgcatcgt ggtacggaat ccctttcaat
    21781 gggcggcgcg cggcaaatgg cgaaattttt gacatgaaca cgcttgtagc ggcgcatcgt
    21841 acgctgccgt tcggcagcat tcttcgcgtc acgaatctga ataatgggcg agatgtggaa
    21901 gtccgcgtca ttgatcgcgg gccctttgtg gggaatcgcg tgttggactt ggcgcgggcc
    21961 gcagccgtag ctctggatat gatcggcacc gggactgctc ccgtgcgtat cgaattgatt
    22021 tctgggtcga atcccacgtc aggagatttc accgtgcaag tcggcgcgtt tgcggatcgc
    22081 gcgaatgcgg agcgcttgcg cgaccggttg ctcgctcaat atcaaccgat ttttatccag
    22141 gaatatgatg tcccggaagg ccatttctac cgcgtccgcg ttgggcgcgt tccaaatccg
    22201 gatgcagcgc agcagcttgc cgcccgcctg aagaacagcg acggattcca aactttcgtg
    22261 atgcggctgg accaaccgaa tgagctaggc attaaataat cggagtgaaa aacttttcac
    22321 tctgcgccat caactctaaa ctctgaattc ggggtgctct catggacgac tgcctgttct
    22381 gcaaaattgg ggaaaagaaa attcccgcaa aattggttta cgaagatgag gaaattttcg
    22441 catttgaaga cattcagccg caggccccga gtcacatcct gctgtgtccg cgcaagcata
    22501 tcgtttcgct aacggaagcg accgcggaag acgcggccat gctagggaag ttgcagttag
    22561 ttgccgcgaa gcttgcaggg gagcgcaacc ttactgccgg ataccgcacc gtttttaaca
    22621 atgggcgcgg ggcatgccag tccgtctttc atctccattt acatctgcta ggcggccgcg
    22681 acttccgctg gccccccgga taggcaaaga gatttaagga aagtaaggaa atacaggaag
    22741 caaaggaagt gtttgtggat caatttcaga ttgcgtgagc taagctgatg gtttcaagat
    22801 cattttgttt tggttcgggt caaatgctct tggaaaaatg gtctcggcgt catattctac
    22861 cccttccatg ttggtccctt ccaggtttgc tccttctagc cgagcgcctt gcagttgcgt
    22921 tgagccacca aggttgtctc gacccaaatt tgcccgtcgc aaatttgcat tcgttaggtt
    22981 agcttttttg aagtcagcac cttgcagttg agctctctca aggtcagcac cctttaggtt
    23041 tgctcgaaaa aagcgacccg aatataaatc agcaccgcga agatttgcgc cttcaagatt
    23101 agagtcatca aagtgcgcgc cctctagtat caatccctca agcacagcct cttgcaggat
    23161 aagatcatca aagttgtagg aatcggcgcc ctgatgaata accttgccat atttattttt
    23221 gatttccatc tcacagacct agccctagaa tttcaattta cggcgattca ttccatcagc
    23281 tacttgtgcg tcattacgta cacaccgtcc caatctccct cggggccggc gaccagatac
    23341 tccttgcagc gattcagata catgcgcgcg gggccatctt ccggccagcg ctccagcagc
    23401 ttttcaaaaa taatttgcgc atcttgccag cgtctctcgc ggtaacacgc tcgtccttgc
    23461 gcaaaaaatt cgagatgttc ttcaaggccg ggcgcatcgc catcgggtgt gccacgcgag
    23521 gctaccaatt catacagcgt gatgggtttt aattttcctt ttacacgaat caaatccaac
    23581 tcgcgaaaaa cgagcaacgg atcttcgact gccgcgtacg tcgcatcgct caccagaata
    23641 tgcgtcgcat attcccggtt gagcccctcg atgcgcgcgg aaaggtttac tgtatcgcct
    23701 agcgccgtat agccgtaccg cagcgccgat cccatgttgc ccacggatgc gctgcccgtc
    23761 gaaagcccga cgccgatgtc gatcaccggc tttccgtccg ctttccattt tttctgcatt
    23821 tcctttagcc gggaaatcat ggcaagcgcg gcatggcagc tttttgtcgc atggttgcga
    23881 tcttcaaatg gcgcccccca gaatgccatc accgcgtctc cgatatattt gtcgagcgtt
    23941 ccatcgaagc ggaagacgat ttgcgtcatg tccgtcaaat attcgttgag cagttcagca
    24001 agttcctgtg cgtccagttt ttcagaaatc gtcgtaaagc cgcgcacgtc gctgaacatt
    24061 acggtaattt ccgttttccg cggtttcacc agatcgggat tttcgagaag gcggcgaatc
    24121 acttccggac ttaaatattg ctggaacgcg ccacggacgc gccgcttttc tttctcttcc
    24181 acaagcgcgc ggtacatcgc aacgaatcca acgttcgcaa ccagcgtgcc cgagggaata
    24241 ataaaattca gccaccaccc atgaagaaac gcgtaccaca cgccaaaact gaacggaaca
    24301 agcagcaaca atccaaaaag catggaccga ggctgtgcca atcccagcca caatcccagc
    24361 ggcactccaa aaagaaatat cgccagcaaa tcgacagcaa cctgattcgc gccgcgaagc
    24421 agcaaatatt tattcaaaat attgtcaatg acgttggcgt gaatctccac ccctggatag
    24481 ttgatccctc catatggcgt cgagcgcaag tcgccgatcc ctgtcgcgga cgctccaacc
    24541 agaacaattt ttcctttaaa tgtaccgggt ggaaatttgt ggcgcactac atccgcgatg
    24601 gaaacgtacg gataggtggc gacgtcaccc tggtagtcga tcatcatccg cccaatggcg
    24661 tccgggtgaa gaatgagcga gggaccgaat tcgactgcgg taactccagt ctcgctgaaa
    24721 tcaagcacca tctgctggtc gggcaatccc tgaaataacc gtacagcttg cacatcgagc
    24781 gaaccgtaaa tatcccaatc gtccagattc ttggagcgcc cgtagggcaa aaccatcagc
    24841 gaatggcgca ccacaccgtc cgcatccggc tcaacattaa aaaatccggt cgcgccgtgg
    24901 cccttgcgca gggcatcgct caagatttcg atgttcgcct gcgcccctct cggcatcaag
    24961 ccgtaaggat cgccaaagcc ttgcatcagg tggcgaagat cgcgttcacc ggtttgcgga
    25021 ttggcggcgc ggacctgcgg gaatggaaaa tcagcaagaa tattggcgta acgatccagc
    25081 gttttttgat ccacgccctt cagatcgtat tcggaataga gaaagaaatt tccaagaact
    25141 acatttccga acctctcgat ggcgcgggca aattgcgcat ctccgtcata ttcctttgag
    25201 agtcgatcca ggtccgcgat cacgcgcggg tctgtagtcc ctccctgcct ttgcctttcg
    25261 attatggttt cgcgcaactc gcgtatgggc gcggccgttt catccggttt gctgaaggtg
    25321 atgtcaaacg cggcgacttt tgctccatct tcacgcagcg cgtcaagcat attcgcaaaa
    25381 tacgtacgcg aaaatggcca gcgtcccagt atttcctgag accgctgatc gatgtccaca
    25441 ataataatgc gcggatcagg atgcttatat cgatcagggc gcagccggaa tctgaggtcg
    25501 agggcattta attcgagccg gttaacaaaa gaaaaaaccg cggtaggacg gtcacctccg
    25561 gtaggacggt cagcaatgaa ggaatatacg taaaccgtaa gagcgaatgc agtgaggccc
    25621 acgctgatgg caaagggtac gcggcgctgc caccacgccc ggatccattc gcgcgggagc
    25681 tgcaaggcgc gacgcggttc actgcccgtg gaatttcctg gcatgggacg ggaattgtag
    25741 ccgagggcga agccgaaggt cagtcacgat acataaaact cagccgcacg cggcctacgt
    25801 cgatcacatc tccatcgtta agttttgtaa ggccatcaat cgttacgcca ttaattttcg
    25861 gcaagcggtc gcctcggcca agatagtagc cgtcctcatg cttattgatt tgcgcagcca
    25921 cttccggagt aaaccagccg cgaagccgca cggtggccat tttggacttg ccgatgacgg
    25981 taagtttgct cgcgattcga tattcctgtt gatcagtatt tccgcgcgaa acgatcagcg
    26041 ttggaatacg aaggcgttcc ggggaaagtt gagatcgctc gccggccgcc acggccgcat
    26101 ccagaagttt acggcgttcg tgggtatcga ggatcatggt ctcattcacg cgcggaaccg
    26161 gcgctctgcg catggcatcc agcgaatgag ttgcatcgtt cgcttgatcc accagaattt
    26221 cgtgtttgcc gatcagaatg gcgtcgccgt ccttgagcat tgcacgttcg acgcggatat
    26281 cgttgagaaa tgagccattg aggctgttca aatcctcgac cacaagcgaa ccagcttcaa
    26341 cataaactct ggcgtggtaa ttcgacactg caagattgtc aatttgaatg tcattatccg
    26401 gagcgcgccc aatcgtcacg gggcgggttc ccaggggaac ctctttcagc aacgaggtct
    26461 cgaatttgag gaccagtctt gacatgatta ttctcctccg gccaaagccg gcgaagcctt
    26521 atccggagta tcgcttccaa atcttaaacc gatcgaaaat gcctgatgat ttttcaatca
    26581 cccgcaaaac aaccacggtc acgttatcca cgcctccatt ttcattggcc aaatcgatca
    26641 aacggtccgc cgattcctgc gccgtactgc tcgtcagaag agtgctcgca atttcagggt
    26701 cggtaaccat tcgcgtcagt ccatccgaac aaagcaggag aaagtctcca accagcaggg
    26761 cttgctcgtc cgtgtctact tcgaccgagc catttgtccc cacagcgcgt gtgaggacgc
    26821 tttgcaattc gctggcatcg gcttcctgtg gcgtcagaat cccaacgcga actttttccg
    26881 cgaccagcga atggtccgcg gtcaactgat ccatcgagcc accgcggagc aaatacgcgc
    26941 gactgtctcc cacatgtgcc agggaaagcc tctgcgcgtc gatccacgcg gccacaatcg
    27001 ttgcgcccat ccctcgttgt tccgggttag aggctgcggt ctcaaatacc ttgcgattcg
    27061 caaggtggat cgcgctagcc agacgattgg ttcgatctga aacgtccgtt ctggactctc
    27121 caaagatggg agtcgtgcgg ctgttttcac cttgccggca atgcgtcaat actgtctgca
    27181 cggcgagatt gctagccact tcgccgtgtg cctcgccacc cattccgtcg gaaagcacaa
    27241 aaagctgaag tgaggagtca atcaaatagc aatcttcgtt atttttccgg acgcgcccaa
    27301 ggtcggtgcg cgcgccgatt tcaatatgca aaagggcctc attcggattg cggtgatctt
    27361 ttgtgttccc aaacggatag cgggacagca ccgcaaactc gcatgacttt accgctgata
    27421 catcgttaag tgagattgct cactaagttt gagactagca aaccgatttt gcgagggcaa
    27481 tagcctgtga cgtaagtagt tacaaccttt aacgacacct gtcctattga cttaatccca
    27541 tgaatttaac gattccgata tacttttctc agcagagagc gtgcggaaaa cccattctgc
    27601 acggttctcc cgcccccttt catgcagcag cgcgccgaaa tttggtattt tgagaactct
    27661 ctggccgcct ctctggctag cccgctgagc cgcgcatatt cagttaaagc ccttccaaac
    27721 cgtccaggca ggcgcacatt ttcggtgccc gagtctaatg atctgcccgt ggtctgtttc
    27781 gccgacctag gtgcgaacga tcttgcggca gtaacgcgcc tggctcggcg ttctccaaga
    27841 attcgcgtga ttggagtttc gcgcaatggc tctgtccaag acacaactgg ctgctttgca
    27901 acgcttccac gcaaggccaa gccaacggtt gttcgcagga cagtagatgc ggcgatcgcc
    27961 aatatcgaac tcacagagcg cgaacgcgcc gcacgggccg atcttgctcg tgcggaacgc
    28021 gaaatggaga tgctgaaccg catcggcatt gctctatccc aaacgcgtga agttggcgca
    28081 ctccttaaca tgattctcat gaaggcaaga gaaatcacgg gggcggatgc cggctcgctt
    28141 taccttgtgg aagaagtaca tacggatgca ttgtccgccg gcccaggtga acgtcgatta
    28201 cgtttcaaac tgacgcaaaa cgatagcgtt caatttccat tcgctgagat tgaacttcct
    28261 ctccaggaaa attctttagc tggatacacc gcattacatg gcaatgtaat taaccttgcc
    28321 gatgcgcgcc agattccgcg caatcgccct tatcgattca acccgcgctt cgatgaagag
    28381 accggatacc gcacgcgttc catgctgact ctgccgatca aaaatggccg tggggaagtg
    28441 atcggtgtgt tgcaactcat caactgcaag cagcatccag aagcgcgcct gacgagcgag
    28501 gaagtggtcg aacgggaagt gctcccattt tccgaccggg cggtgcgcct ggcgctttcc
    28561 ctggcttctc aggcgggcgt ggcctatgaa aatagcaggc tctaccagga tattgaaaaa
    28621 ctattcgaag gtttcgtgca ggcctcggtg aaagcgatcg aacagcgcga cccgacaacc
    28681 tcgggacatt cgctgcgcgt ttcccagatg acggttggcc ttgcggaaat tgtcgatcgt
    28741 gtggagacag gttcctatgc ttccattcgg ttttctcccg ggcaaatgaa agaaattcgt
    28801 tacgccgcgc tgctgcatga tttcggaaaa gtcggcgtgc gcgaagaagt cctgatcaaa
    28861 gcgaaaaagc tttatcccat gcagctcgat gttatttcgc atcgcttcga ttacctttac
    28921 atggagttgg aggcgcaact cgagcgcgaa aaagttcgta tcctcatgga aatggaccgt
    28981 cccgaagcct tatcccgcat tgcttcactg gaagagaaat atcggcggcg taaactcgag
    29041 atggaaagca gctttgaggc tgtgctgcag gcgaatgaac caaccgtcct tccggagggg
    29101 aaattcgatc gcctgctgga aattggccgg cagacttatc atgatccgca tggcgtcgaa
    29161 aaaccgattc tgactgcgga agaagtccgc tacctttcaa ttcccaaagg cagccttagc
    29221 gcggatgagc gcgcccagat tgaatcccac gtgattcaca gtttcaattt tctgcttcaa
    29281 attccatgga ccaagcagat tcgcgaaatt ccccgaatcg ttcgcgcgca tcatgagaaa
    29341 ttgaatggga cgggctatcc ttacaaattg cgcagcaatg aaattcctgt gcaggcgaag
    29401 atcatgacga tttgcgatat tttcgatgcg ctttctgcct cggaccggcc ctacaaaaag
    29461 gccgtggccc ctgagcgcgc tctcggtatc ctcgaaatgt ccgtgaaaga tcaggaactc
    29521 gacccggaat tgtataaact cttcctcgat gcagggatct ttcgcctcgc ggataatgtt
    29581 tgaaaataaa atgtggttcg cggttttcga taaatccctc tgaatcgttt aagattgaga
    29641 ccgtttcgtt tcttttgcac gcagcgaagc atcggtagcg cggcaaatac tgccttatta
    29701 ttttgcgcgg aagaaacctt ggcgaaaaac aacgttgaag ccccagactg gtcaaccatc
    29761 ccgccaccgg tggacgatgg cgcagcatcg caccttatcg gcatgagcgt ggcctccgtt
    29821 tcgcttcccg ctaccgacgg aagcataatc gacctttcta ctctccaagg aagaactgta
    29881 gtgtatgcgt atccgcgaac aggaagacca gggatagaaa atccggaagg gtgggaccag
    29941 attcccggag cgcggggttg cacgccgcaa acctgctctt ttcgagacca ttttgcggaa
    30001 atcaaatccc tgggcgtcga ttttattttt ggtctttcga cgcaaaacac cgcttatcag
    30061 cgggaagctg ccgaacgcct gcacctgcca tttccgattc tctccgacga gaagctggtc
    30121 cttgcatcgt ccatgaggct tccagttttt gaaaccagcg gcatgaaact gctgaaacgt
    30181 ttgacgctgg tcatcgataa cggcgtcatc gaacacgttt tctatcccgt gtttccgccc
    30241 gatcaggacg cggcaaatgt gatcggttgg ctgaaaacga aaagttaatt tcatggaata
    30301 aaatcaagaa aagaggtttt gagatttcgc gcttttcatc ctgccgcgat agaatccctg
    30361 acatgcttca tccgttcatt tccaataaaa agttggctcg catctccctg ttgatttcca
    30421 tgctgtcctg ctgcgctgta cgggcagcag ctcaacagcc gacaaaaccg catgcag
//
LOCUS       DASZMB010000064        13843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4735045, whole genome shotgun sequence.
ACCESSION   DASZMB010000064 DASZMB010000000
VERSION     DASZMB010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 13843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13843
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4735045"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VG272_02850"
     CDS             <1..578
                     /locus_tag="VG272_02850"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF12647.1"
                     /translation="DGIGLWTSYLDKWPGDPMFAPVYEELNRRKAIIFLHSAPPSCCR
                     ALQPGVADSVVEYDFDIARAVVSYLKSDSFRRYSNIRFIFPHSGGTLPVLANRVSETL
                     PEKRSEKDSAEIMDELKGVYFDVAHAAYPAPLAAITKVIPISQLLFGSDYPIYPPPVT
                     EGPLDRFGFSSTDLQAINRGNAERLFPRLKA"
     gene            609..1811
                     /locus_tag="VG272_02855"
     CDS             609..1811
                     /locus_tag="VG272_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWF12648.1"
                     /translation="MNRKIMKFGLAILFVAALATFAGLRHGTQASDSKNSAMPPASIP
                     TFSMENVGREAFFYVGGKYEGAPGKEEMHGAMYVEVMVPKQIKQKYPIVFFHGNGQTG
                     AVWKQTPDGRPGWAYFLINQGYVVYMVDYPARGRSPYVPDMDGKLGLRTAQDLSQIWT
                     GPTLSGGNFPRMKLYTQWPSDSPKKGTMGDPVFDNFIKGQMQFISAQAELAVPAGVAL
                     LDQIGSPVILITHSQGGGIGFNVADQRPNLVAGMVAIEPGGPQIGVVDTATVKYTGKI
                     GNAWGITNMPMHYDPPVNRPDDLKTYLEEKSDRPDEVPCYMQKEPVHKLVSLEKLHIL
                     SISAEGTYHRVFDACIPKWLNQAGVKDDFIRLEDVNIHGNMHEMFLDRNSDVVIKFID
                     EWLRKNVK"
     gene            complement(1998..2615)
                     /locus_tag="VG272_02860"
     CDS             complement(1998..2615)
                     /locus_tag="VG272_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HWF12649.1"
                     /translation="MLFGDTGERKKLEQQAREEGRLPPGQSLTLKWPVLHAGNVPRVD
                     LSTWDFRIDGLVEKSVRLTWEEFNRLPQTEIQCDMHCVTHWSRLDNSFEGVLFTEVMK
                     LTAPKPEAKFALVRGENGFTANLPLEDLMQPTTIFAFRHDGEPLTAEHGYPVRLVVPH
                     LYLWKSVKWVRGFALTAEDTPGFWEEYGYHMRGDPWKEQRFNPEQ"
     gene            2803..3783
                     /locus_tag="VG272_02865"
     CDS             2803..3783
                     /locus_tag="VG272_02865"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HWF12650.1"
                     /translation="MPARILSGNAIRDQIYAELKQEIAALSAAGIRPGLAAVLVGENP
                     ASQIYVRSKIAACEQLGLGSWLHTPPATVTTDEMLALVAELNARDDVDGILVQVPLPS
                     QVDTKRVLEAVDPAKDVDGFHSMNLGRLVSGRPGLVSCTPAGCVEILRRSGIPLEGAN
                     SVVIGRSDNVGKPMALLLLHTNSTVTICHSKTRDLPQVVRRADIVIAAIGRPAMVEPD
                     WIRPGATVIDVGINRITNATEAESIFANFPAKLAQFKKKGSVLVGDVHPGVADVAGAI
                     TPVPGGVGPLTIAMLMSNTVKAARLRREHSASKNTTKTAGNISGTAAAKL"
     gene            3783..4427
                     /gene="coaE"
                     /locus_tag="VG272_02870"
     CDS             3783..4427
                     /gene="coaE"
                     /locus_tag="VG272_02870"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HWF12651.1"
                     /translation="MLSVGLTGGIATGKTAVVAMLRELGCRVLEADKIAHQMIEPDGP
                     AYEAVVREFGRGILTPEGLVDRQKLGPIVFADPRRLARLNAVVHPPVLAEQSRQLEAI
                     GQADPHAIAVVEAALLIEAGFDKKLESIIVTWCTPEQQLERLTAPGTGRGLSVEQARQ
                     RIAAQMPVEEKRRRADEVIDCSGTLEHTRAQVIALFAKLKGMEGAKSREIRTGS"
     gene            4563..5714
                     /locus_tag="VG272_02875"
     CDS             4563..5714
                     /locus_tag="VG272_02875"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWF12652.1"
                     /translation="MREGKDSRGVFRLLALALVLVLGAYWAGSRWGERQPRSVGAVPA
                     SFPEPKPGDLLGDEAIYVKIYSQSAPAVANIVTRTLEYDVFMEPVPVEGAGSGFVIDP
                     RGYILTNFHVVQNAQAISVTLGDRTHYDAKFIGADERNDIALVKIDPKDKKLPVLTMG
                     DSESLMVGQRVLAIGNPFGFQSTLTTGVVSALGRTVQTGQTTVIDGAIQTDAAINQGN
                     SGGPLLNSHGEVIGMNSAIYTPSGTTAGIGFAIPISTARQIAQDLITDGRVHRASMGV
                     EARLLTPQVAEALHLPVSEGLMIERVTPGGPAANAGLKGGDRQAILGMRRILLGGDVV
                     TGIDGRPITAQADLTLALNRKRPGDTVTVEYYRGSQKMTAKVTLNDASR"
     gene            5787..6365
                     /locus_tag="VG272_02880"
     CDS             5787..6365
                     /locus_tag="VG272_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12653.1"
                     /translation="MKPNVYIIAGPNGAGKTTFAREFLPYYANCTNFINADLIALGVA
                     PLSPETAAIRAGKLMISEIALHSRRCLDFGFETTLSGYGHLKSIQRLIRAGYRTHIFF
                     LWVPKIELALDRVRVRVLGGGHDVPEVVVRRRYSRSIRNFLELYRFQTETWTLFDNSA
                     ETPKILAFGGKDKTSIIMEDEYKDILLQYGEK"
     gene            complement(6564..7337)
                     /locus_tag="VG272_02885"
     CDS             complement(6564..7337)
                     /locus_tag="VG272_02885"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWF12654.1"
                     /translation="MKTSPEQVEQRVPVHYDTRLPKLTLLQRTLIPIIGWAVWFVIRV
                     LGPTIRFEILGGQKAGRDYRPDLPPNIFAFWHRCIIPSAWYFRNRNGVLMNTTNFDGQ
                     WTRRVIERLGFGTAQGSSTRGGLRGLAVMAKRLAEGVDAGFTIDGPRGPRNVAKPGPV
                     MLARRSGRPIVLFHVGLERAWTLKKSWDLMQIPKPFTRAVLIIAPVIQVSKNADHEEI
                     ERKHQEMQKMLERLRDVAESWFSLSEEERKIERRLWNSY"
     gene            7421..8494
                     /locus_tag="VG272_02890"
     CDS             7421..8494
                     /locus_tag="VG272_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12655.1"
                     /translation="MRALKIPRSRLAAWTKVAAAALLAYAGAFSAHIARISAQDFPAI
                     IGRIEGDDVEVVTKTPTGTETDAGSTVVANGSDVTLHSGHALLLLSTGGEISICGPAH
                     FKMVKSSGALTLALDYGRVHPSLESSDAFTIYMPTMVATPIAIGGGPRDMTVGLEQGG
                     EMCINTARGAMRVEPQFSDQSMIIPQGGTANLAEGQIVSLHADASACSCEFPRARLQT
                     PKTAPPSQSTAPQSDREIATLARPMPPDRKKPEPKQPASTAASEPVYTVLMPPLSFDA
                     KSPEPPPDPSPETILLVREVRVHPSVEFQGHVNPAPAPPPIPAAPVAPAVAEPPPAPR
                     PAKTETGFFGRVRAFFHRLTGSN"
     gene            8622..10850
                     /locus_tag="VG272_02895"
     CDS             8622..10850
                     /locus_tag="VG272_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12656.1"
                     /translation="MLSLLDLRLHTRAQRGIFSAHRDTGFPATSRFLIFASLLLSLYF
                     SSCGAVGSAPAPVTINVTPTSAQPFPGGNVQFQAAVENAASSAINWQVNLIPGGNAIV
                     GTISSSGFYIAPATVPNPPAVTVTAALQSEPSLTASAIVTVQSLSAVQGPLTLSPKLF
                     SITTSQSIQLNVLTAGVNNTDVNWAVDGATNGNLTVGTISSSGDYSPSLAAGPHLITA
                     ILKANPGAIGSATIEVTSFPGTLTWRNDNARSGINSEELALAPATISLSTFGKLFSCP
                     VDGYIYAQPLYVPNLYVPNLVIPGRGTHNVLFVATENDSVYAFDADAKPCVLLWHISL
                     IPSDWQAIATPSLDITSTDIVPFVGITGTPVIDINASTLYVVAAIQTVGTLPAYRQRL
                     YALDLATGTPKIEPSGVAITSPAGDPFEYSPMHQNQRAALLLDNGTVYVAFGSFGEEP
                     EYHGWLFAYDAGSLNQTGSFEVTPGGVQGGIWQSGGGPSADSNHNVFVITGDGTFNAG
                     RPEINYSYGNSFLRLANAGGLPLADYFTPCDEAAGQILGTTAPILLPDSAGSSLQPHL
                     LIGGSKGGSLYVVNRDNMAGYVGPCPDASTRVQTIPVGTAIFSTPLFWNSNVYVAPAN
                     ANLMSFAMSGGVVASTPLSAQSPEQLGAQGATPVISSNGTANAILWLIDTSGAQTWPT
                     ESTPAILRAYDPNNLSNEIYNSARVTPPDGLAVKFTVPTVANGKVYFGTQTELDVYGL
                     LK"
     gene            complement(10927..12585)
                     /locus_tag="VG272_02900"
     CDS             complement(10927..12585)
                     /locus_tag="VG272_02900"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HWF12657.1"
                     /translation="MPVERERLDADVLIVGAGPAGLACALYLSQLIAQHNASGAKPEL
                     SAENIYVLEKAREIGAHQLSGAVMDPRGLAELVPDWEKTAPLESPVTDDAAYVLTSSS
                     SYKLPITPPPLQNHGNYIVSLNKLVKWLGGLVEKSGVNLFTQFSGRNLIYEGGGIAGV
                     ITEDKGVDKDGKPKDNFTPGYELRAKVTVLAEGPRGSLTKEMVSRLKLDGLNPQVYGI
                     GIKELWDVPADRIKPGFVAHTLGWPLDTSMYGGGWLYGLPGNRVSVGMVIGLDYHDPR
                     FDPHAAFQKYKTHPFVKRVLEGGKMVRYGAKTVPYGGWYSMPRQFVDGGLIIGDSASL
                     LNSQRLKGIHIAIKSGMLAAETVFEALKTGEATAKTLSSFPQKIEKSWIKKELWAVRN
                     FHQGFRHGLYAGLFHAGIQFVTGGRGLIDPMRTRPGHEEYHKLNGSVPAPERFKGDGS
                     LTFDRLTDVYHSGTRHEEDQPCHLIVSNLNVCATKCVVEYGNPCQYFCPAAVYEMVQE
                     KGQPRLKINASNCVHCKTCDIADPYEIITWVPPEGGGGPNYEGM"
     gene            complement(12651..13553)
                     /locus_tag="VG272_02905"
     CDS             complement(12651..13553)
                     /locus_tag="VG272_02905"
                     /inference="COORDINATES: protein motif:HMM:NF005875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HWF12658.1"
                     /translation="MEIKKVGVLGCGLMGSGIAQVSATAGFDVTVLEVDQKYIEKGFS
                     AIRKSLEKLAAKGAVSEPVEKIFARLRGTTRKEDLANCDFVVEAVIENLDEKRKMFAS
                     IDPIVKPSAIFASNTSSISITEIMTATKRPGQFVGLHFFNPVPLMKLVEVARTIVTTN
                     EAFETACAFGTKLGKVPVRTSDKTGFIVNRLLVPYLMDAIRAFEEGVGSVADIDASMK
                     LGCGYPMGPFTLLDFVGLDTTYYITHVMFDEFKEKRFSSPSLLKRMVMAGWYGKKNGK
                     GFYDWSNPEKPVPQDAALRGITKD"
BASE COUNT         3146 a         3885 c         3708 g         3104 t
ORIGIN      
        1 cggatggcat cggcctgtgg accagctatc tcgacaagtg gcccggcgat cctatgttcg
       61 cgcccgtgta cgaggaactg aaccggcgca aggccatcat ttttctgcac tccgcgccgc
      121 ctagttgctg ccgcgcgttg cagccgggag tggccgacag cgttgtggaa tacgatttcg
      181 atatcgccag agcggttgtg agctatctga agagcgattc gttccgccgc tactcgaata
      241 tacgcttcat tttcccgcac tccggtggaa cccttcccgt cctggccaat cgtgtgagcg
      301 aaactctgcc agaaaaacgc tcggagaaag actccgcaga aatcatggat gaattgaagg
      361 gagtctactt cgacgtcgcg cacgcggcct atccggcgcc actcgccgcg atcacgaaag
      421 taattccgat ttcgcagctt cttttcggga gcgactatcc catctatccg cctcctgtga
      481 ccgagggacc tctcgatcgt ttcggatttt cgtccaccga tttacaagcg attaatcgcg
      541 gaaacgcaga gcggctattt ccacgtctta aggcataggc ttttagcgag cactttttgg
      601 aggaatgcat gaaccgtaag attatgaaat ttggattggc aattttgttt gtggccgcgc
      661 tcgccacgtt tgccgggctg cgccacggca cgcaagccag cgactcaaag aattccgcga
      721 tgccgcccgc ttcgattccc acattctcca tggaaaatgt aggccgcgaa gcattcttct
      781 acgtcggtgg aaaatacgaa ggcgctcccg gcaaggaaga aatgcacgga gcgatgtacg
      841 tggaagtcat ggtgcccaag caaatcaagc agaaatatcc catcgtcttt tttcatggga
      901 acggacagac cggagccgtc tggaagcaaa ctcccgacgg ccggccgggc tgggcgtact
      961 tcttgatcaa tcaaggctat gtcgtttaca tggtggatta cccggcgcgc ggacgttcgc
     1021 cttacgtccc tgacatggac ggcaagctcg gcttgcggac cgcgcaagat ctgtcgcaaa
     1081 tctggacagg gcccacgcta tccggcggaa attttccgcg tatgaaactc tacacacagt
     1141 ggccgagcga ctcgccgaaa aaaggaacga tgggcgaccc ggtgttcgat aatttcatca
     1201 aaggccagat gcagttcatt tccgctcaag ccgaacttgc cgtgcccgca ggcgtcgcgc
     1261 tgctcgacca aattggcagc cccgttattc tgatcacgca ctcgcaggga ggcggcatcg
     1321 gctttaacgt tgctgaccag cgcccgaatc ttgtggcagg catggtggcg attgagcccg
     1381 gcggtccgca aatcggcgtc gtggatacgg ccacggtgaa gtacaccggc aagatcggaa
     1441 atgcatgggg catcaccaac atgcccatgc actacgatcc tccggtcaac cgccccgacg
     1501 atttgaaaac atacctcgaa gaaaaatcgg atcgcccgga tgaagttccc tgctacatgc
     1561 agaaggaacc tgtgcacaaa ctggtgagcc tcgagaaact gcacatcctg tccatctccg
     1621 cggaaggcac gtatcaccgt gtgttcgatg cgtgcattcc caaatggctc aatcaggcag
     1681 gcgtgaagga tgatttcatt cgcctcgaag acgtcaacat ccacggcaac atgcacgaaa
     1741 tgtttctcga ccggaacagc gatgtagtca tcaagttcat cgatgagtgg ctgcgcaaaa
     1801 acgtgaagta atgcattctg tagcacatct cttcagaggc agggatttag aggatggcct
     1861 attcagcgcg tgaaagttgt gcgtcggatg ccagcttggg agccgctgat ccggcatcgc
     1921 gcgctgccat ctaaacattg tcgtttaact ggaatctaaa ccctcgcctc tgaagaggcg
     1981 agctacaaag ggcagaatca ctgctccgga ttaaatcgct gctctttcca gggatcgccg
     2041 cgcatgtggt aaccgtactc ttcccagaat cccggcgtgt cctctgcggt gagcgcaaaa
     2101 cctcgcaccc acttcacact tttccaaaga tatagatgcg gcacaaccag gcgcacggga
     2161 tagccatgct cggcggtgag cggttcccca tcgtgccgaa atgcgaaaat cgttgtcggc
     2221 tgcatcagat cttcgagggg cagattggcg gtgaatccat tttcgccgcg cacgagagcg
     2281 aatttcgctt cgggtttggg cgcggttaat ttcatcactt ccgtgaacag gacgccttcg
     2341 aaggaattgt cgaggcggct ccagtgcgtc acgcagtgca tgtcacactg aatttccgtt
     2401 tgcggcaggc ggttaaattc ttcccaggtg agacgaacgg atttttcgac gagtccatcg
     2461 atgcgaaaat cccacgtgct gagatccact cggggcacat ttcctgcgtg cagcacaggc
     2521 cacttcagcg taagcgattg tcccggcggc aggcggcctt cctcgcgggc ctgctgttcc
     2581 agtttttttc gttcgcccgt gtcgccgaaa agcatggtcg cctcctttgc gggagaaatt
     2641 aattgtacat gaaaagaagc caggcctgaa tttggcgggt cggctggaaa tctgtgcgcg
     2701 acgctttcag taaaaccgcc ggaaagatgc cggcgccacg ggtggttcat ttgctgtgct
     2761 accattaata cgagccattt aacgttcgag caaggagcca ctttgccagc gcgcatactt
     2821 agcggcaacg cgattcgaga ccagatttat gcggagctta agcaggaaat tgctgctttg
     2881 agcgccgcgg gaattcgtcc tggactcgcg gcggtgctcg taggcgaaaa tcccgcgtcg
     2941 cagatttatg tgcgcagcaa aatcgcggct tgcgagcagt tgggacttgg gagctggcta
     3001 cacacgccgc ctgctacggt aacaactgat gaaatgcttg cgctggttgc ggagttgaat
     3061 gctcgcgacg acgtggacgg gatacttgtt caagttcctc ttccttcaca ggttgacacc
     3121 aagcgagtgc tcgaagccgt ggatccggcg aaagatgtag atggttttca ctcaatgaac
     3181 cttgggcgtc tggtttcagg ccgaccggga ctcgtctcat gcacacccgc cggatgtgtg
     3241 gaaattctgc ggcgcagcgg cattcctctt gagggagcaa attcggtcgt tattggccgc
     3301 agcgataacg taggaaaacc catggctctg ctgctattgc acaccaattc gacagtgaca
     3361 atctgccatt ctaaaacgcg cgatttgccg caagtagtac ggcgcgccga tatcgtaatt
     3421 gctgcgattg gtcgccccgc catggtcgaa cctgattgga tccggcccgg cgcaacggta
     3481 atcgacgtag gcatcaatcg cattacaaat gcaactgaag cggagagcat tttcgccaat
     3541 tttccggcaa aactcgcgca attcaagaag aaaggctcgg tccttgtggg ggacgttcat
     3601 ccgggcgtcg cggacgtggc cggagcgata actcctgtgc ctggcggcgt aggccctctt
     3661 acgatcgcca tgttaatgag caacacggtg aaagcagccc gcctgcgacg cgaacattcc
     3721 gcatccaaga atacgacgaa aacggctgga aacatttccg gcacggctgc ggctaaactc
     3781 tgatgctcag cgtcggccta accggaggaa ttgccaccgg caagacggcg gtggttgcga
     3841 tgttgcgcga acttggctgc cgcgtactcg aagcggacaa gattgcgcat caaatgattg
     3901 aaccggatgg gccggcgtac gaagcagtcg tccgcgaatt tggtcgtggc atcctcaccc
     3961 cggaagggct cgtggaccgg caaaagcttg ggccgatcgt gtttgccgat cctaggcgcc
     4021 tcgcgcgact gaacgccgtc gtgcatccgc ctgtcctcgc tgagcagagc cgccagcttg
     4081 aggccatcgg gcaggctgat ccgcacgcca tcgccgtagt ggaagcggcg ttgcttatcg
     4141 aagcaggctt tgataaaaag ctggaatcca taatcgtcac ctggtgcacg ccggaacagc
     4201 aactcgagcg actcacggct ccgggaacgg gacgcggttt aagcgtggag caagcgcggc
     4261 aacggatcgc ggctcaaatg cccgtggaag aaaaaaggcg cagagccgat gaagtgattg
     4321 attgctccgg cacgctggag cacacgcgcg cgcaggtcat tgcgctcttc gccaaactga
     4381 aaggcatgga aggcgcgaaa tccagagaaa taaggacggg aagctaaagc gctgtgccaa
     4441 atgttgtgta acacttgcct tcaggagaac actcggtcag aacgtgtgct cgtctgaaag
     4501 cgatcgctac aattaatttt taaattgtag gcgttagatt tagttgttgg agggacacgg
     4561 ttatgcgtga aggaaaagac agccggggcg ttttccgctt gctggctctg gccctggtgc
     4621 tcgtgctagg tgcttattgg gcgggcagcc gctggggcga acgccagccg cgaagcgtag
     4681 gcgccgtgcc cgcttccttt cccgagccaa aaccgggaga tctgctgggc gacgaagcga
     4741 tttacgtcaa aatttacagc cagtccgccc cggccgtggc caacattgtg acgcgcacgc
     4801 tcgaatacga cgtgttcatg gaaccagttc ccgtcgaagg cgcgggctct ggattcgtca
     4861 tcgatccgcg cggctatatt ctgacgaatt tccacgtcgt gcaaaatgct caggcgattt
     4921 cagttacgct gggtgaccgc acgcattacg acgcaaaatt tattggcgcg gatgaacgca
     4981 atgacatcgc gctcgtgaaa atcgatccga aagacaaaaa attgcccgta ctgacgatgg
     5041 gcgattccga aagtttgatg gtgggtcaac gcgtcctggc tatcgggaat ccatttggct
     5101 ttcaaagcac gctgaccacc ggcgtcgtca gcgcgctcgg acgcacggtt cagacaggtc
     5161 aaaccaccgt gattgatggc gcgattcaaa ccgacgccgc cattaatcag ggaaattccg
     5221 gcgggccgct cctgaattcc cacggagaag taatcggcat gaactccgcg atctacacac
     5281 cgtccggcac aactgccggt atcggttttg caatccccat cagcaccgcg cggcaaatcg
     5341 cgcaggattt aattacagat ggccgcgtgc accgcgcttc catgggcgta gaagcacgcc
     5401 tgctgacacc ccaagttgct gaggcgctgc atctgcccgt cagtgaagga ttgatgatcg
     5461 aacgcgtcac gcccggcggc ccggccgcaa atgcgggcct gaaaggcgga gatcgccagg
     5521 caattctcgg catgcgccgg attttgctgg gaggcgacgt tgtgactgga attgacggcc
     5581 gccccatcac agctcaagcg gacctgactc ttgctttaaa ccgtaaacga cccggagaca
     5641 cggtgaccgt ggagtattac cgcggcagcc aaaaaatgac ggccaaagtg acactgaacg
     5701 acgccagccg ttaagcgttc gttgaataga agcataacaa gaaaatagcg ctttcaaaat
     5761 tatgttcttg caacgtttca gttatcatga agccaaacgt ctatatcatc gcgggtccaa
     5821 acggcgcggg aaagacgaca ttcgcccgag aattccttcc ttattatgcg aattgcacca
     5881 atttcatcaa tgcggacctc atagcgctcg gagttgcacc tttatctccg gaaaccgccg
     5941 cgatacgagc gggaaaactg atgatcagcg agattgctct gcattccaga cgatgccttg
     6001 actttggttt tgaaacgact ctatctggct atgggcacct caaatcgatc caacgtctca
     6061 ttcgagctgg atatcgaaca catatttttt tcctttgggt gccaaaaata gagttggcgc
     6121 tggaccgagt gagagtacga gtcttaggtg gtggacatga cgtgccggaa gtagtcgtgc
     6181 gtcgtcgcta tagtcgttcc atcagaaatt ttttggagtt atatcgcttt cagacagaaa
     6241 cgtggacatt gttcgataat tcggccgaaa ctcccaaaat cctggccttt ggagggaagg
     6301 ataaaacgag tataatcatg gaggacgaat ataaagatat cttgctacag tacggggaaa
     6361 aatgatgaag ccccaagctg atatattaga attgccgcta gttacaaggg cggagattgc
     6421 cttaaaagct gccgtcgaaa aggcgatgga acgacatgct cgcgagggac tccctgtcta
     6481 cgtcttacgg ggtggagagg taattgaaat atctaacgaa gagttaaaaa tacgatatcc
     6541 gcgttatcag gccaaatctt gaattaataa gagttccaaa gccttctttc aattttccgt
     6601 tcttcttccg aaagtgaaaa ccagctttcc gccacatctc tcagccgttc cagcattttc
     6661 tgcatttcct gatgctttct ttcgatctct tcatgatcgg cattcttaga aacttgaatg
     6721 actggcgcga taatcagaac ggcgcgcgta aaaggtttgg gtatctgcat caagtcccat
     6781 gacttcttca gcgtccaggc tcgctccaat cccacgtgaa ataaaacgat aggcctgccc
     6841 gagcggcgcg ccaacataac gggccccggc ttcgccacat tgcgcggacc gcgcggaccg
     6901 tcaatcgtga agccagcgtc gactccctct gcaagccgct tagccattac ggcgaggccg
     6961 cgcaatccgc cgcgggtcga gcttccctgc gccgtgccaa aacccaagcg ctcaatcaca
     7021 cgacgggtcc actgcccatc gaaattggtt gtgttcatga ggacgccatt acgatttcga
     7081 aagtaccagg cggaaggaat aatgcagcgg tgccaaaatg caaaaatatt cggcggcaaa
     7141 tccggccggt agtcgcgccc cgctttttgc ccacctaata tctcgaaccg aatggtcggc
     7201 ccaagcacgc gaatcacgaa ccagacagcc cagccgataa tcggaatcag cgtacgctgg
     7261 agaagagtaa gtttgggcag ccgtgtatcg taatgaaccg ggacgcgctg ctctacctgt
     7321 tctggtgatg ttttcacttg gtccggcatg cagttatagt ataagtcgaa gacggcatga
     7381 agatagccac acaactcgct tcaggaagct gtaggtaaac gtgcgtgctt taaaaatccc
     7441 gcggagccgc ctggctgcct ggacgaaagt tgccgccgcc gcattgttgg catacgcagg
     7501 cgcattttcc gcccacattg cgcgcatttc cgcgcaggat tttccagcca ttattgggcg
     7561 catcgaaggc gatgacgtcg aagtggtgac aaaaaccccg acaggaaccg agacggatgc
     7621 gggttcaact gttgtagcaa acggcagcga cgtcacattg cattccggcc atgcgctcct
     7681 gttgctgagt acaggcggcg aaataagcat ttgcggaccc gcacatttca agatggtcaa
     7741 atcttccgga gccctgacgc tcgccttgga ttatggccgc gtgcatccat ccctggaatc
     7801 gtccgacgct ttcacaattt atatgccgac gatggttgcc acgcccatcg caattggggg
     7861 aggcccgcgc gacatgacgg tgggcctcga acagggcgga gagatgtgca tcaacacggc
     7921 gcgcggcgcg atgcgcgtgg agcctcaatt ttccgaccag agcatgatta ttccgcaagg
     7981 aggcacggcg aatctggcgg aggggcagat cgtttccctg catgcggatg cttccgcttg
     8041 ctcgtgcgaa tttccgcgtg cccgtctgca aactcccaag actgctccgc catcgcagtc
     8101 gactgctccg cagtctgatc gtgaaatcgc aacgctcgcc cggcctatgc ctcccgaccg
     8161 gaagaaaccc gaacccaagc agcctgcttc gacggccgcg agtgaacctg tatacacggt
     8221 gcttatgccg ccgctcagtt ttgacgcgaa atctcctgaa cctccgcccg atccttcgcc
     8281 tgaaaccatt ctcctcgtgc gtgaagtgcg cgtgcatcct tccgtggaat ttcaaggcca
     8341 tgtgaatccg gccccggctc ctccgccgat tccagccgcc cctgtcgctc ccgctgttgc
     8401 tgaaccgcca cccgccccac gtccggcaaa aacggaaact ggattcttcg gccgcgtgcg
     8461 cgcctttttc caccggctta ccggatcaaa ttaacatcct tcgatcatcg tgactttgca
     8521 tgacaaatgt actgtcatga atcatgacta ccgtcattga cgcctctttg agcctgtgat
     8581 acgttccgcc tgctttcaga agcctgactt ctccagagcc catgctcagc ctattggatc
     8641 tgcgacttca cactcgcgcg cagcgcggaa ttttctctgc gcatcgcgac accggattcc
     8701 cggccacgtc acgcttttta attttcgcct ccctgctgct ttctttgtat ttcagttcct
     8761 gcggagcagt cggctcggca cctgctccgg tcaccataaa tgtcacgcca acatctgctc
     8821 aaccttttcc cggcggcaac gtgcaattcc aagccgcggt ggaaaatgca gcgagttctg
     8881 caatcaattg gcaggtgaat ctgatacccg gcggaaatgc gatcgtagga acaatttcat
     8941 catcgggctt ttacattgcc cccgccacag tgccgaatcc gcctgccgtt acggtgaccg
     9001 ctgccctgca atcggaaccc tccctaaccg catccgcaat tgttacggtt caaagcctca
     9061 gcgcggtcca agggccattg acgctttcgc ccaagctttt cagcatcacc acatcgcaat
     9121 ccattcaact aaatgtgcta acagcaggag tgaacaacac ggacgtcaac tgggccgttg
     9181 acggcgccac aaacggaaac cttacggtgg ggacgatctc ctcatccggg gattactcgc
     9241 cctcgcttgc ggcaggcccg catttaatta ccgcaatctt gaaagcaaat cccggcgcga
     9301 ttggttcagc caccatcgaa gtcacaagct ttccgggtac gttaacctgg cgaaacgaca
     9361 acgctcgatc gggaataaac agcgaggaac tggcgctcgc gcctgctacc ataagtttgt
     9421 ccacatttgg aaaactattc tcctgcccgg tagatggcta catctacgcg cagccgttgt
     9481 acgtgcccaa tttgtacgtg cccaatcttg ttattccagg caggggaacc cacaacgtac
     9541 tttttgtcgc tacggaaaat gattccgtat atgcatttga cgcggatgca aagccttgcg
     9601 tactgctgtg gcatataagc cttattccat cggactggca ggccatcgcc acccccagcc
     9661 tggatattac gagcacggat attgtccctt ttgtgggaat taccgggact cccgtgatcg
     9721 atataaatgc gtctacgctc tacgtggttg cagcgatcca gacggttgga accttgccag
     9781 cctataggca gcgcctctat gcgttagatt tggcaacagg cacgccgaaa attgagccat
     9841 ccggagtcgc cattacttcg ccagcgggag acccgttcga atactctccc atgcaccaaa
     9901 accaaagggc tgcgttgctg ctggataacg ggaccgttta cgttgcattc ggttcgtttg
     9961 gggaagaacc tgaatatcac ggatggctgt ttgcatacga tgcaggctct ctaaatcaaa
    10021 ccggcagttt cgaagtcact cccgggggtg tccaaggagg aatttggcaa agtggcggcg
    10081 ggccgtcagc ggattcgaat cacaacgttt tcgtgattac gggagatgga acgttcaatg
    10141 ctggacgtcc cgaaattaat tacagctacg gtaacagctt cctgcggctg gcaaatgccg
    10201 gaggcttgcc gctcgccgat tactttacgc catgcgatga agcggcaggg cagattctgg
    10261 ggacaaccgc gccaattcta ttgccggatt ccgcaggctc ctctttgcaa ccgcacttgc
    10321 tgattggcgg ttcgaagggc ggctccctct atgtagtcaa ccgcgacaac atggccgggt
    10381 acgttggccc gtgccccgat gcctcgacac gcgtacaaac aattcctgta ggtacggcga
    10441 ttttcagcac tcctttgttt tggaacagca acgtgtatgt cgctcccgct aacgcgaact
    10501 tgatgtcgtt cgcaatgtcc ggtggagttg tcgcttcgac tccgctttcc gcacaatccc
    10561 cggaacaact cggcgcacaa ggcgctacgc ccgttatttc ttcgaacgga acggccaatg
    10621 ccattctttg gctgatcgac acaagtgggg cacagacttg gccgacggag agtacgccgg
    10681 ccattctgcg ggcatatgac ccgaataatc tttcgaacga aatctacaac agcgcgaggg
    10741 tcactcctcc agatggactg gcggtgaagt tcacggtacc aacggtcgct aacggcaagg
    10801 tttacttcgg tacccagacc gaattggatg tgtacggcct gcttaagtag gcttcagctc
    10861 gtttcctttt gaattttgca gccttcaccg acaccgaaat cagacaggct gacgcctgtc
    10921 ctacactcac atcccctcgt aattcggccc gcctccgccc tctggaggca cccaggtgat
    10981 gatttcatag gggtcggcaa tatcgcaggt cttgcagtgc acgcagttcg aagcattaat
    11041 ttttaatcgc ggttgcccct tctcctgcac catctcgtag acggccgcgg ggcaaaaata
    11101 ctggcaagga tttccgtatt cgaccacgca cttcgtcgca caaacattca gattcgaaac
    11161 aatcagatgg cagggttgat cttcttcgtg gcgcgtgccg gaatggtaga cgtcggtcag
    11221 acggtcaaac gtcagcgacc cgtcgccctt gaaacgctcc ggcgcgggaa ccgagccgtt
    11281 cagcttgtga tactcctcgt gcccgggacg cgtgcgcatg ggatcgatca gaccgcgccc
    11341 gcccgtcacg aattgaattc ccgcatggaa taatcccgcg tagagaccgt gccggaaacc
    11401 ttgatgaaaa tttcgcacgg cccacaattc ttttttgatc cagctttttt cgatcttttg
    11461 cggaaatgag gagagagttt tcgcagtcgc ttcgcctgtt ttcagagctt caaaaacggt
    11521 ttccgcggca agcattccgc tcttgattgc gatatgtatg cccttcagtc gctgcgaatt
    11581 gagcaggctt gcggaatcgc cgatgatcaa tcctccatcc acaaattggc gcggcatcga
    11641 ataccagccg ccgtagggca cagtcttcgc gccatagcga accattttgc cgccttcgag
    11701 cacgcgtttc acgaacggat gagttttgta tttctgaaac gctgcgtgcg gatcaaaacg
    11761 cggatcgtga taatcgaggc cgatgaccat gccaacggaa acgcgattcc ccggcagacc
    11821 gtacagccat cctccgccat acatgctggt gtcgagcggc cagccaagcg tgtgcgccac
    11881 aaatccaggc ttgatccggt ccgccggcac atcccacagt tcctttatgc cgattccgta
    11941 gacttgcgga ttgaggccgt cgagtttcaa ccggctcacc atttcttttg tgagcgatcc
    12001 gcgtggacct tcggcaagca cggtcacttt agcgcgcagt tcgtaacccg gcgtgaaatt
    12061 atctttcggc ttgccgtctt tatcgacgcc cttgtcttcg gtgattacgc cggcgattcc
    12121 gccaccttcg taaattaaat ttctgccgga aaattgggtg aataaattta cgcccgattt
    12181 ttccacgagg ccgccgagcc atttcacaag cttgttcagt gagacgatat agttgccgtg
    12241 attttgcagc ggaggaggcg tgatcggcaa tttgtaggaa cttgaggaag tcaaaacgta
    12301 agcggcgtca tcggtgactg gagattcgag aggcgcagtc ttttcccagt cgggaactag
    12361 ttccgcgaga cctcgcggat ccatcaccgc gccggaaagc tggtgcgctc cgatttcgcg
    12421 agccttttcg aggacgtaaa tattttcggc ggaaagctcg ggcttcgcgc ccgatgcgtt
    12481 gtgttgagca atcaattgtg aaaggtagag cgcgcaagca agcccagcgg gacccgcacc
    12541 aacgattaag acatccgcat ccagccgctc tcgttcgaca ggcatatctt catcctagca
    12601 agttcgcggg cacaaatccc cgtatcgatt tcatggttaa ttcgattcgg ttaatccttc
    12661 gtaatccctc gcagcgcggc gtcctgcgga actggctttt ccgggtttga ccaatcgtaa
    12721 aaacctttgc cgtttttctt gccgtaccaa ccggccatca ccatgcgttt cagcaaggag
    12781 ggagaagaga aacgcttttc cttgaactca tcgaacatca cgtgagtgat gtagtatgtg
    12841 gtatcgagcc ccacgaaatc gagcagcgta aatgggccca tcgggtagcc gcagccgagc
    12901 ttcatcgatg cgtcgatatc ggcaactgag ccgacaccct cttcaaacgc gcgaatcgcg
    12961 tccataagat agggcactag caagcggttt acgatgaagc cggtcttgtc gctcgtccga
    13021 accggaactt ttcctaattt cgttccgaat gcgcaggccg tttcaaatgc ttcgttcgtc
    13081 gtgacgattg tgcgcgcaac ctcgactagt ttcatcagcg gcacaggatt gaaaaagtga
    13141 agtcccacaa attggccggg gcgcttcgtc gccgtcatga tttccgtgat cgatatggag
    13201 gacgtgttgc tcgcaaaaat ggccgaaggt ttaacgatgg gatcgatgga agcaaacatt
    13261 ttccgttttt catcgaggtt ttcgatgact gcttccacaa caaaatcgca attcgcaagg
    13321 tcctctttac gagtagtccc ccttagtcgc gcgaatattt tttccaccgg ttcggagact
    13381 gcgccttttg cggcaagctt ttcgagagat ttgcgaatcg cagaaaaacc tttttcgata
    13441 tatttctgat cgacctccaa aacggttacg tcgaatccag ccgtggcgga tacttgcgcg
    13501 attccggaac ccatcaaccc gcaaccgagc acgccaactt tcttgatctc cattacgcga
    13561 ccctcttttc ccgcggaatt ttcgccaggc gcacggcgat atcatagcgc aatttgcaaa
    13621 caggccatcg acgtctgggg ccatccaatg caaaatttct gaaacgaaaa tatttggact
    13681 tatatggctc tgttggcttc ctcaaggatg gtcggtctgc tgtagcccgc ctcttcagag
    13741 gcgggggttt agattccccg ctggcaggct cgacgtgtaa actctaaacc cccgcctctg
    13801 aatgaaaagg cgggctacag cagcccgaac catcttgtgg aag
//
LOCUS       DASZMB010000065        10178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3629524, whole genome shotgun sequence.
ACCESSION   DASZMB010000065 DASZMB010000000
VERSION     DASZMB010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 10178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10178
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3629524"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1189
                     /locus_tag="VG272_02910"
     CDS             185..1189
                     /locus_tag="VG272_02910"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HWF12659.1"
                     /translation="MKKIAIIGGGAWGTALAIALTRSSRAHNLSLWAHSADVVETIIA
                     RRINEIYLSGYELPASVEVTGDLAQALADAAIVLGVMPSAHAREIYRSMRRHVGPESV
                     FVSATKGLEPATHTRMSEVIAEEINLQNSSRVAVLSGPSFAEEVAAGDPTAIVLAAAD
                     AKLASEIQEEFSGPRFRLYTNDDVIGTEIAGAVKNVIAIASGICTGLDLGTNAAAALV
                     TRGLAEMTRLAVALGGRRETLAGLAGVGDLILTTTGELSRNRSVGIQLGKGRNLKDIL
                     GSTRRVAEGVGSAAATLELAQRAAVEMPITEQVHSVLTAGCVTRDAIRDLMERRLKQE
                     "
     gene            1410..3107
                     /locus_tag="VG272_02915"
     CDS             1410..3107
                     /locus_tag="VG272_02915"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWF12660.1"
                     /translation="MLLVSVLPLTLYHLEVLQLSQTKLTDTEGVQQTEVTRSLGDEIQ
                     QFDASLYQQLISERQILALTDLIDKVDDSQSAPQVSRVLENFVASNPNILYLTAVGKE
                     AKGTGAGNFQANQDPFVSKALQRAFSTTMQSLVFRSDPLAIGPENRPAFVMAVPLQVN
                     DQFSGMLAAVVSLDGILKRLQETSVRGRVVFVVDHNGQIVAHPDMRQFVPGSDAKPSS
                     PVVQQVAGVPKGLRTTETLRRFVTSEDGNKAEMIGTYSTIPDLNWAVVAQRGLDKARE
                     DAGVKELNAQALKFVVVVTFAALILGYFFAVGISTPIRALAASTLAISRGEFHQRTPV
                     KGAAEISELAETFNHMAGDIEQFVVQLRQAADQNHELFLGSIRMLAAAIDEKDPYTRG
                     HSGRVAKYSILIAQQLGLGAEELEKIRISALLHDVGKIGVDDRVLKKPGALTPEEFSL
                     MKQHPLKGANIMRPVAQLKEMLPGIELHHEHVDGGGYPHGIQGDEIPLMARIIAVADT
                     LDAMTTNRPYQTAMDLEFAMNRIVELANTKFDAAVVDALSRVVQAGKIRLNAALVEA"
     gene            complement(3604..4089)
                     /locus_tag="VG272_02920"
     CDS             complement(3604..4089)
                     /locus_tag="VG272_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HWF12661.1"
                     /translation="MFSVRMNSVFALFLLSVFAGAACWAQMPAKMDADSAAIKQCVAA
                     WDDAWNRHDARATAMAYVEDGDFSSTTGVPSHGWKELESHYNDIFTTFLKDAHRTDTV
                     RSIRFLTPEIAAVDIDWQMTGAKTRDGKDVPTRKGLLNWVVTKQHNGQWMITIYHESA
                     F"
     gene            complement(4129..4602)
                     /locus_tag="VG272_02925"
     CDS             complement(4129..4602)
                     /locus_tag="VG272_02925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HWF12662.1"
                     /translation="MISTQTRIVIVALLCFTFSAAGSRAQMAASRDADTASIKQSVAS
                     FADTWNSHDAHAVAMRYAEDGDFSSVKAEPSHGRKELEDHYNTIFTTFLKNAHTTDTV
                     RSIRFLAPDLASVDIDWLVTDPAAPGGVLRKGLLNWIMSKRNGQWMIVIYHEQAF"
     gene            4999..5925
                     /gene="ftsY"
                     /locus_tag="VG272_02930"
     CDS             4999..5925
                     /gene="ftsY"
                     /locus_tag="VG272_02930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HWF12663.1"
                     /translation="MLSLFNNKEDDEKKSKEGGSLLDRLKQSISKTRNQITTKVEDLI
                     SGEKQIDPEMLTKLENALLAADLGPRTTHQILEAVRQKLERHALSNASDLKTELKNQL
                     LHVLRTVPSTNGNATAAKEPHPLVIFVVGVNGTGKTTTIGKLAYRLHKDGKNVVLAAG
                     DTFRAAAIEQLGVWATRTGSEIVKTKSGGDPAAVVYDALSSARARNADIVIVDTAGRL
                     HNKQNLMAELDKMKRTATKVIPDAPHEVLLVMDATTGQNGLSQAREFTATAGVTGIVL
                     TKLDGTAKGGIVVAITRELGLPVQYVGTGESI"
     gene            6098..7279
                     /gene="ribD"
                     /locus_tag="VG272_02935"
     CDS             6098..7279
                     /gene="ribD"
                     /locus_tag="VG272_02935"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HWF12664.1"
                     /translation="MSSSTSTTTAPTVNADTVYMERALELAAGGIALTSPNPMVGAVL
                     VRDGIVIGEGFHTYDGIKHAEILALEAAGESARGATLYINLEPCCHTGRTGPCTQAVI
                     TAGVARVVAAMADPNPSVAGRGFKQLRSAGIEVSAGLREAEAKLLNEAFARWILSHKP
                     LVTLKSALTLDGQLVLPHALNRGDKRRRMPLKDRWISSPESRSEVQSMRHASDALMTG
                     IGTVLVDDPFLTDRTELPRRRKLLRVIMDSQLRLPLRSNLVRTSDADVLVFTRRKENS
                     LKARALRRAGVEVVRLTRRGLQPDLIEAIEELGRRNILSVLLEAGSILNSAAVAAGIV
                     DKMRVFFAPKLAGSAMKSPRVNSASARIQPVKELQNVTIEPSGPDFAIEGYLRDVYRN
                     R"
     gene            7260..7946
                     /locus_tag="VG272_02940"
     CDS             7260..7946
                     /locus_tag="VG272_02940"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HWF12665.1"
                     /translation="MFTGIVEHVGTIESVELCDVGARSDFGSTRTDQIGATIRVNAGP
                     LIVDLEKSGSIAVNGCCLTAIDISAATFAADLSGETLRRTSLGHMKPGMRVNLERPLT
                     AMKELGGHFVQGHVDGVGRVVRLEPEGANWWFGVQIPAELARYVAMKGSIALDGISLT
                     VAAWHDGSVETAIIPYTYEHTNLSSLKVGDAVNVECDILAKHVDRILEARQESAVSRL
                     TVAKLIEEGF"
     gene            complement(7989..9818)
                     /locus_tag="VG272_02945"
     CDS             complement(7989..9818)
                     /locus_tag="VG272_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWF12666.1"
                     /translation="MGRKIMRAGLAFLSIISLLPVRVFAQDQQAGGQMQSQPAPQAAP
                     PAAVPPRTVNMNLDYSAGRKWFPNITEQYKPVTIPMPALTNSPRLDQLLQNGKLMLSL
                     QDTISLALENNLAIDVERYTPWLNEVNLLRARSGVNGLVPFDPTVTGSLNLQDSVTPL
                     NNPLFAGVIPSGTTTPTNQAPPAFVQHIGNVNVQYNQNFLMGTQFQVGMTNNRTSSNF
                     GFFNLYNPDVQSALSVTITQPLLRGFGVLPNTRLIIEAKRSIKVGLSQLEQQVMATTT
                     QVSNDYWELVYARENVKVEEAALGVSQQLFDENSMRLQIGTLSSLDVLTAQSQLASDK
                     QALVQAQSVQLQDETTLLNDITKDPLAPALMGAEIIPTTMISTPGTGENVEIADAVKE
                     AWQKRPELKQASLNLENAGTDVKATKNELLPSVNLFGEYIASGLGGIQTVTSSGGVVT
                     VFPGGATQDLTRVFNGNYSTFEGGINFTLPIRNRAAQADNAQALLAERQQKTQYRQEQ
                     NAIFVSVRNALIAMREDRASLAAAEEAKKLAVQTLQDEQEKYHLGASTSYNVVLRSRD
                     VTTAEGTELRDRINLLEDELKFNQAMGRTLEVNQITLNDALKP"
     gene            complement(9868..>10178)
                     /locus_tag="VG272_02950"
     CDS             complement(9868..>10178)
                     /locus_tag="VG272_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12667.1"
                     /translation="SAVALNKIPHAVNSKRLVRIAQAPLRPHARNLAFRAPISSTSTV
                     NCVVALAWAHLYTGAHADRTELFIGFRGIGFSLWVLGLARTKLAQAEAYATGFRKLIV
                     "
BASE COUNT         2329 a         2811 c         2774 g         2264 t
ORIGIN      
        1 tagccaaaca tcgcgacaac attgaacggc tgctggcggg gacggaaccg cgcttcgaga
       61 tgggccgcaa gagacgtgaa ttgtgacccg tgatttgtaa ttcgtgattc gtaatttgaa
      121 attcaaattt gagatttatg attcctgaac gatagcgaag ctcccgcaag gggctgtctc
      181 attcatgaag aaaattgcca tcattggagg cggggcgtgg ggaacggcgc tagcaatcgc
      241 gctgactcgc tcctctcgcg cgcacaatct ttcgctgtgg gcgcacagcg ccgacgtcgt
      301 ggaaacaatt atcgcgaggc gcatcaacga gatctatctt tccggctatg aattacccgc
      361 gagcgttgaa gttacaggcg acttggccca ggcacttgcg gatgcagcca tcgtattagg
      421 cgtaatgcca tcggcccacg ctcgcgaaat ttatcgatcg atgcgccggc acgtcggccc
      481 tgaatccgtt ttcgtcagcg cgacgaaagg ccttgaacca gcgactcata cccgcatgag
      541 cgaggtgatc gcggaagaaa taaacctgca aaatagttct cgcgtcgccg ttctctccgg
      601 cccgagtttc gctgaagaag ttgccgccgg agatcccacg gccatagttc tcgcggccgc
      661 ggacgctaag cttgcctcgg aaatccagga agaattttcc ggcccgcgtt tccgcctcta
      721 taccaacgat gacgtcatcg gaacggaaat tgccggcgct gtaaaaaatg tgattgcgat
      781 tgcttcaggc atttgcacgg gtttggatct aggtactaac gcggcggcgg cgcttgtcac
      841 acgcggactc gcggaaatga cgcggctggc cgtggcactc ggcggccggc gcgaaacgct
      901 tgccggcctt gcgggcgtgg gcgacctgat tctcacgacc actggtgaac tcagccgcaa
      961 ccgatctgtg ggcattcaac tgggcaaagg ccgtaacctc aaagatattc tgggctcgac
     1021 gcgcagggta gccgaaggcg taggaagcgc ggcggccact ctggaattgg cgcaacgagc
     1081 ggcggtcgaa atgccaataa ccgaacaggt tcattcggtg ctgacggcag gatgcgttac
     1141 gcgcgacgcc atccgcgacc tcatggagcg gcgcctgaaa caagaataat gctcccgcgg
     1201 ttgccgcatt tcctgaataa caagtactct gttcacagca atggcatcag accccttaat
     1261 tcatgcgtag catggcgttt ttccctccta ctaatgcgcc atcccgaatc gtaaaataag
     1321 agtgatagga cctgcccata ttggacctaa gtagattcaa atcgatcttt tctcaccggc
     1381 cgaggattct ctggctggtg ctgggtgcca tgctgcttgt cagcgtcctg cccttaaccc
     1441 tctatcatct tgaggttctt caacttagcc agactaaact gaccgatacg gaaggcgtcc
     1501 agcaaaccga agttaccaga tcgctcggcg acgaaattca gcaattcgat gccagcctgt
     1561 accagcagct cattagcgag cggcaaattt tagcccttac ggacctgatt gataaggtcg
     1621 acgactccca gagtgctccg caagtttcac gcgtacttga aaattttgtg gccagcaatc
     1681 ccaatatttt gtacctgacc gctgtgggca aagaagcgaa aggaaccggc gccggaaact
     1741 tccaggcgaa ccaggacccc ttcgtatcca aagctttgca gcgcgcgttt tcgaccacca
     1801 tgcagtcact cgtgtttcga agcgaccccc tggcgattgg gcccgaaaat cgacccgcct
     1861 tcgtgatggc tgtgccgctg caagtgaacg atcaattttc gggaatgctg gccgccgttg
     1921 tatcgctgga tggaatcctc aaaaggctcc aggaaaccag tgtgcgcggg cgagtcgtgt
     1981 ttgtggtgga ccataacggc caaatcgttg cgcatcctga catgcgccaa ttcgtgcccg
     2041 ggtcggacgc aaaacccagt tcgcccgttg tccagcaagt agctggcgtg ccaaaaggac
     2101 tgcgcacgac cgaaactctt cgcaggtttg taacttcgga agatggaaat aaagctgaga
     2161 tgatcggaac atacagcacg attcctgacc tgaattgggc cgtggtggcg cagcgcggct
     2221 tggacaaggc gcgcgaagac gcgggcgtaa aggaactcaa tgcgcaagct ctaaaattcg
     2281 tggttgtcgt cacgttcgcg gcgttgattc tcggatattt ttttgcggtt ggtatcagca
     2341 ctcccattcg cgcgctcgcc gcatccacac tggctatcag tcgcggagaa tttcatcagc
     2401 ggacgccggt gaaaggcgca gctgaaatca gcgaactcgc cgaaacgttc aatcacatgg
     2461 ctggggacat agaacaattt gtcgttcagc tgaggcaggc cgcggatcaa aaccatgagc
     2521 tgtttcttgg ctcgattcgc atgctcgccg cggccatcga cgaaaaggat ccgtacacac
     2581 ggggccattc cgggcgcgtg gcgaagtatt ccattctcat cgcgcaacaa cttgggttgg
     2641 gtgccgagga actggaaaaa attcgtattt cggcgctgtt gcacgacgta ggaaaaatcg
     2701 gagtcgacga ccgtgtgctg aagaaaccgg gcgcgctcac gccggaagag ttttctttaa
     2761 tgaagcagca tcccttgaag ggtgcgaata tcatgcggcc tgttgcgcag cttaaggaaa
     2821 tgctgcccgg aattgaactg caccatgaac acgtcgatgg cggcggctat ccgcacggaa
     2881 ttcaaggcga tgaaattccc ttgatggccc gcatcatcgc cgtggccgat acgctcgacg
     2941 cgatgaccac caatcgtcct taccagacag cgatggacct tgaatttgcc atgaaccgca
     3001 ttgttgagtt ggcgaataca aaatttgatg cggcggttgt ggacgccctg agccgcgtcg
     3061 tacaggctgg aaaaataaga ttaaacgcag cgcttgtgga agcgtgagat tttgattcct
     3121 taaagtggca tgagcttcag agtctgagtg gctctgctga catccccggt tccgctgccg
     3181 atctgctgta gcccacctct tcagaggcgg gagtttaaac gatgatctac tcaaaacctg
     3241 aaataagggt gccgatggtt ggactacggc ctgaaggccc cagacacagg cgctgaacag
     3301 gaaattttgg tcacactttc acctaaaccc tcgcctctga agaggcgagc tacaattcca
     3361 gcaccacaaa acgggcgaaa cttactgcag catgcaggat atcaacagag ccagtctgag
     3421 tgagaaccgc agaatcgttg tgggtcgtgg ctttcgccac gacataagtc ttgcaaaatc
     3481 ggtttgcttt tgggctgtgg ttgcaatgtc gcagttctca cacagactct tcagcctgtg
     3541 ggtttcttgc aagaaccaaa tcccacaggc tgaagcctat gccacttaaa cctcaaacaa
     3601 aatttagaat gcggattcgt ggtaaatcgt aatcatccat tggccgttgt gctgtttggt
     3661 cactacccaa ttgagcaggc ctttgcgtgt ggggacgtct ttaccgtccc gcgtttttgc
     3721 gccggtcatt tgccagtcga tgtcgacagc ggcaatctcc ggagtcaaaa aacgaatact
     3781 tctgacggta tcggtccggt gggcgtcctt cagaaacgtg gtgaagatat cgttgtagtg
     3841 cgattccagt tctttccacc cgtggctggg aacgccggtg gtgctcgaaa aatctccgtc
     3901 ttccacatag gccatggccg tcgctcgcgc gtcatggcga ttccacgcat cgtcccatgc
     3961 ggcaacacat tgcttgatcg cggctgaatc cgcgtccatt ttggctggca tctgcgccca
     4021 gcaggctgcg ccggcaaata cagagagcag gaacaacgcg aataccgaat tcatacggac
     4081 tgaaaacata atgccctcct gattttcaaa cgcacaaaac cagttaagtt aaaaagcttg
     4141 ttcgtggtaa atcacgatca tccactggcc attgcgcttg gacataatcc aattgagcag
     4201 gcctttgcgc agaactcctc caggcgcggc ggggtccgtc accagccaat cgatatccac
     4261 ggacgcgagg tctggcgcaa gaaatcggat gctcctcacg gtgtcggtcg tatgcgcgtt
     4321 cttcagaaaa gttgtgaaaa tcgtattgta gtgatcttcg agttccttgc ggccatggct
     4381 gggctcggct ttcacacttg aaaagtcgcc gtcctccgca tagcgcatcg cgacggcatg
     4441 cgcgtcgtga ctgttccaag tatcagcgaa cgaggctacg gattgtttaa tggaggcggt
     4501 atccgcgtcc cgactcgcag ccatctgcgc gcgggaacct gctgcggaaa acgtgaaaca
     4561 caacagggcc acgatcacga ttcttgtttg cgttgagatc atctcgcacc tccaggaatt
     4621 aaatttggca atagacttgg gccgaatccg gaaccgtgcc gattggcggc atcatataac
     4681 aaccatccag aatttgcttt cgtaaaggcc gtccagcttt tggacgggcg acctgctttg
     4741 agtctttcag gcgggcaatt ttgcgtgtgc ggaacagcgt gccctctccg tcccgaaaag
     4801 cgtgggccgg agagtccact gcgaaaccgt gtcgcattgc ttttgctttc gtagagtccg
     4861 tccagctttt ggacgggcga ctttctttga gttaaattca aatcggtcca tcgtttgcta
     4921 agaggccacc gcgtaagtag gttttcttct gctagaatac agcattcagc acttgcactt
     4981 attttggcgt gaaaaaacat gttgtcactc tttaacaaca aggaagacga cgagaagaag
     5041 tccaaagagg gcggctcgct gctcgaccgc ctgaaacaat ccatatcgaa aacacgcaat
     5101 cagattacga ccaaggtcga ggacctgatc tcgggcgaaa aacagatcga tcccgagatg
     5161 ctgacaaaac ttgaaaatgc gctgcttgcc gctgacctcg gcccgcgcac cacgcaccag
     5221 attctcgaag ctgtgcggca gaaactcgaa cgccacgcgc taagcaacgc atcggatctt
     5281 aaaacggaat tgaaaaacca attgcttcat gtgttgcgga cagtgccatc cacaaatgga
     5341 aacgcgaccg ccgcgaaaga accgcacccg ctcgttattt tcgtggtggg cgtgaatggc
     5401 acagggaaaa cgaccaccat cggcaaactt gcttaccgcc tgcacaagga cggaaaaaat
     5461 gtagttctcg cggcaggcga tacgtttcgt gccgccgcca ttgagcagct cggtgtatgg
     5521 gccacgcgca caggttcgga aattgtgaag acaaaatccg gcggcgatcc ggcggcggtt
     5581 gtctatgacg cgctttcttc cgcgcgcgcg cgtaacgccg atatcgtcat cgtcgacacc
     5641 gcgggccgcc tgcacaacaa acaaaatctg atggccgaac tcgacaaaat gaagcgcacg
     5701 gccacgaaag taattcctga tgcgccgcac gaagttctgc tcgtgatgga cgcgacaact
     5761 ggacagaatg gcctttctca ggcgcgagaa tttaccgcca ccgctggcgt cacgggcatt
     5821 gtgctgacca agcttgatgg cacggccaag ggcggaatcg tagtggccat cacgcgcgaa
     5881 cttggcttgc ccgtgcagta cgtaggcaca ggcgaatcga tctaagatct ggtgccgttc
     5941 gacgcggaaa cttttgtcac ttcccttttt gattgagatc gcgcgattta cgtggcatag
     6001 gcttcagcct gtggaatttg ggccttgcaa ggactaaaac tcacaggcca aagcctacgc
     6061 cacggaaatc aatgcggaaa cactcggatt tatgattatg agcagttcta catctacgac
     6121 caccgcgccc acagtcaacg ccgataccgt ttacatggaa cgcgcgctcg agttagccgc
     6181 gggtggaatt gcgctcacta gtccaaatcc catggtcggc gcggttctgg tacgcgacgg
     6241 catcgtaatc ggcgaaggtt tccacacgta cgatggcatc aagcatgccg agatccttgc
     6301 gttggaagcc gccggtgaat ccgcgcgagg cgcaacgctt tacatcaacc tggaaccttg
     6361 ttgccacacc ggtcgcaccg gcccatgtac acaagccgtg attaccgcgg gcgtagcgcg
     6421 cgtcgtagct gcgatggcgg acccgaatcc ttcggtggct ggacgcggct tcaaacaatt
     6481 gcgctcggct ggcatcgaag tcagcgcagg tttgcgtgag gccgaagcga aacttctgaa
     6541 tgaagcgttc gcgcgctgga tcctttcgca caaacctttg gtcacactca aatccgctct
     6601 cactctcgac ggccaacttg ttctgcccca tgcactgaac agaggagaca agcgacggcg
     6661 catgccctta aaagaccgct ggatttcttc cccggaatcg cgctccgaag tgcaaagcat
     6721 gcgccacgct tcggacgctt tgatgactgg cataggcacc gtgctggtgg atgatccatt
     6781 tctgaccgat cgaacggagc tgccgcgccg ccgaaaattg ctgcgcgtaa ttatggattc
     6841 gcaactgcgc ttgccgctgc gctccaatct ggtgcgcaca tccgatgctg atgtactggt
     6901 tttcacacgt cgaaaagaaa attcgctgaa agcacgcgcc ctgcgccgtg ccggtgtcga
     6961 agtggtgcgt cttaccaggc gcggcttgca gcctgacctg atcgaggcca tcgaagaact
     7021 tggccgtcgc aatattttga gtgtgctgct ggaagcaggc tcaatcctaa attcagctgc
     7081 cgtagccgcc ggcattgtgg ataaaatgcg cgtgttcttc gcgccaaaac ttgccggctc
     7141 tgcgatgaaa tctccacgcg tgaattcggc atccgcgcgg attcagcccg taaaagaatt
     7201 gcagaacgtg accatcgaac catccgggcc ggatttcgca atcgaggggt atctccgcga
     7261 tgtttacagg aatcgttgag cacgtcggca ccatcgaatc ggtcgaattg tgcgacgttg
     7321 gcgcgcgctc cgatttcggc agcacgcgca ccgaccagat tggcgcgacg atccgcgtaa
     7381 acgccggacc gttgatcgtt gatctcgaaa aatcggggag cattgccgtc aacggctgct
     7441 gtctcaccgc tatcgatatt tcggccgcaa cgtttgccgc cgatctttcc ggtgaaacgc
     7501 tgcgccgcac cagcctcggg catatgaagc cgggcatgag ggtcaatctt gagcgtccgc
     7561 tgaccgcgat gaaagaactt ggcggccatt tcgtgcaagg ccacgtggac ggagtcggcc
     7621 gcgtcgtccg gctcgaaccc gaaggcgcga attggtggtt cggtgtgcaa attcccgccg
     7681 aactcgcgcg ctacgtcgcc atgaaaggtt ccatcgcgct cgacggaata agcctcacag
     7741 tagccgcatg gcacgacggc agcgtcgaaa ccgccatcat tccgtacacc tacgaacaca
     7801 ccaacttgtc ttcgctaaaa gtgggcgacg ccgtaaatgt ggagtgtgat atcctcgcca
     7861 agcatgtcga ccgcatcctg gaagcccggc aggaatcagc agtgtcgaga ctcaccgttg
     7921 caaaattaat tgaagagggt ttttaggaca ggccgggttg caatcagcct ccaacattgg
     7981 agttcgcact aaggcttcag cgcatcgttc aacgtgattt gatttacttc cagggttcgg
     8041 cccatggctt ggttgaattt cagttcgtct tccagcaaat taattcggtc gcgcagctct
     8101 gtgccttcgg cggtggtgac gtcgcgcgac cgcaggacga cgttgtagct ggtcgacgcg
     8161 ccgagatggt atttttcctg ctcatcctgc aaggtttgca cggccagctt cttcgcctcc
     8221 tcggcggcgg cgagcgaggc gcggtcttcg cgcatggcga tcagcgcatt tcgcacgctc
     8281 acgaaaattg cgttctgctc ctgcctgtat tgcgttttct gctgacgctc ggccagcaac
     8341 gcctgcgcgt tatccgcttg cgccgcgcga ttgcgaatgg gcagcgtgaa attaattccg
     8401 ccctcgaacg tcgagtaatt gccgttgaat acgcgggtca ggtcttgcgt cgcgccgcct
     8461 ggaaaaacgg tgactacgcc tcccgaactg gttaccgtct ggattccacc gagtccggaa
     8521 gcgatatatt ccccgaagag attcactgag ggcagcaatt catttttcgt ggccttcacg
     8581 tcggttcccg cattttcaag attcaaggaa gcctgcttca gctcaggcct tttttgccat
     8641 gcctctttca cggcatcggc gatctccacg ttttcgcccg tgcctggcgt cgaaatcatg
     8701 gtggtcggaa taatttccgc gcccatcagt gccggtgcga gcgggtcttt cgtaatgtca
     8761 ttcagcaaag tcgtttcatc ctgcaattga acggactgcg cctgcaccag cgcctgcttg
     8821 tccgaagcca gttgcgattg cgcggtcagc acatcgagcg aagaaagtgt gccgatctgc
     8881 aggcgcatgg aattctcatc gaaaagctgc tgcgaaaccc ctagcgccgc ttcctcgact
     8941 ttcacgtttt cgcgcgcgta gacgagttcc cagtaatcat tagatacttg cgtcgttgtg
     9001 gccatcacct gctgctccaa ctgggacaga cccaccttga tgctgcgctt ggcttcgata
     9061 attaaccggg tgtttgggag aacaccgaat cctctcagca atggctgcgt gatcgtcaca
     9121 ctgagcgccg actgcacatc gggattgtac aaattgaaaa atccaaaatt cgaagaagtg
     9181 cgattattag tcattcccac ttgaaactgc gtgcccataa gaaaattctg gttgtattga
     9241 acattcacat ttccaatgtg ctgaacgaat gctggcggag cctggttcgt cggcgtcgtt
     9301 gttccgctgg gaatgactcc ggcgaaaagc gggttattca gcggcgtcac cgaatcctga
     9361 aggttaagac ttcccgtaac cgtcgggtca aaagggacta aaccgtttac gccggaacgg
     9421 gcgcgcagca aattcacttc gttcagccac ggcgtgtacc gctccacgtc gatcgccaga
     9481 ttattttcca gtgccagtga aatggtgtcc tgcaaactca gcatcagttt tccgttttgc
     9541 agcaattgat cgaggcgcgg agaatttgtc agcgccggca tgggaatcgt caccggcttg
     9601 tattgctcgg tgatgttggg aaaccatttt cgccccgctg aataatccag attcatattt
     9661 actgtgcgcg gcggaaccgc ggccggaggt gcggcttgcg gcgcaggctg gctttgcatc
     9721 tgcccgcctg cctgctgatc ctgcgcgaaa acccgcaccg gaagcagaga gatgatcgaa
     9781 agaaatgcca atccagcgcg cataattttc ctacccatta tttccccctc ctcgatatga
     9841 ggacttattc caaatagaaa aagtaaccta aacgatgagt tttcggaaac cagtggcata
     9901 ggcttcagcc tgtgcgagtt tggtccttgc aagtcccaaa acccacaggc tgaagcctat
     9961 gccacgaaaa ccaataaaca attcagttct atcggcatgc gctccggtgt ataagtgtgc
    10021 ccacgcaaga gccacaacac aatttacagt tgacgtactc gatatcggcg cgcgaaacgc
    10081 caaatttcgc gcatggggcc gaagaggagc ctgagcaatg cgaaccaatc gtttgctatt
    10141 aactgcgtgt ggaattttat taagtgcgac ggcacttt
//
LOCUS       DASZMB010000066        36496 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3630262, whole genome shotgun sequence.
ACCESSION   DASZMB010000066 DASZMB010000000
VERSION     DASZMB010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 36496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36496
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3630262"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VG272_02955"
     CDS             complement(<1..629)
                     /locus_tag="VG272_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HWF12668.1"
                     /translation="MNCKLAKRFVPGYLDGELDLSRTIEMETHLQVCAECARELERQQ
                     ALRAALQRGSLAFGAPEGLRERVQTSLLAAVAAPAPTPTPASVQTKVPKRAGQWSPFQ
                     FLRWASAFAVLALCSVVAWQLLPGFHRPTNEQQLAAEVFTSHVRSLEANHLMDVVSTD
                     QHTVKPWFDGKLDFSPPVEDLASDGFPLVGGRLDYLEGRQVAALVYQRR"
     gene            complement(631..1161)
                     /locus_tag="VG272_02960"
     CDS             complement(631..1161)
                     /locus_tag="VG272_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF12669.1"
                     /translation="MQGTAARERFEQAVLPHLDAAYNLARWLTRNDEDAQDVTQESFL
                     RAFRFFDGYQGGNMRAWLLTIVRNTCYTWLHQNRPAEPAVEFDEEIHSEEFSGEADPE
                     LQLLANADKATLQCALGELPEMFREVLVLREIEGMSYKEIADVASVSIGTVMSRLARA
                     RTRLRQSLSAALGKEL"
     gene            1343..2308
                     /locus_tag="VG272_02965"
     CDS             1343..2308
                     /locus_tag="VG272_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWF12670.1"
                     /translation="MADFIRDNINKDGIDRRGFLQCMAWAGTGVLYSVHGGILKSLPL
                     MQGAANAYDMAKMKGADFSFIQISDSHIGFSKEANPDVTATLQVAVDRINSAAAPPDF
                     LLHTGDISQLSKPSEFDTAEQVLKGAKASKIFYVPGEHDVLTDNGQQYLDRFGKDTKG
                     NGWYSFDHKGAHFIGLVNVMDLKAGGLGSLGSDQLKWLQDDVRNLKSSTPVIVFAHIP
                     LWTVYPEWGWGTDDSEQALGYLKRFGSVSVLNGHIHQIMQKVEGNVTFHTALSTAFPQ
                     PAPGTALSPGPMKVPADQLRSVLGLSHVNYVQGNHALALVDSTLA"
     gene            2354..2719
                     /locus_tag="VG272_02970"
     CDS             2354..2719
                     /locus_tag="VG272_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HWF12671.1"
                     /translation="MMKSIKLICAMAAILTTALALGAAARPGLAGNENDKPASAAEIK
                     IDNFSFGPTTLTVPAGTTITWKNNDDVPHVVSSDDGKMFKSKALDTDDRFSFTFTKPG
                     TYNYYCAIHPKMTAKIVVQ"
     gene            2719..3177
                     /locus_tag="VG272_02975"
     CDS             2719..3177
                     /locus_tag="VG272_02975"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HWF12672.1"
                     /translation="MRGDSDESVKSVLLAKHAQHVALVHFPIALIFVSAAFDLLSLRK
                     ENLALLRAAYYTVIAAAIAAVPTVATGILAWQFQFEGKVPRGNLKLHMFLAFFSSVLI
                     WLVCGLHVREHRKLEAGSPNLRLAVEFLAVALTLLTAHVGGFVSGVNGPA"
     gene            3810..5735
                     /locus_tag="VG272_02980"
     CDS             3810..5735
                     /locus_tag="VG272_02980"
                     /inference="COORDINATES: protein motif:HMM:NF045198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12673.1"
                     /translation="MNNLLSLHSEIEGKHFKLWLTSEPVLLRVLNAGIFHDSQAHLER
                     IRLRLRRYVQNPSFKRARSVLERSHYCIIAGIPGIGKTTLAEVLLADLVDRKGFETFR
                     ISRGLDEIGPIKNTKRKQAFYFDDFLGTTSVDKLQKNEDRRLIDLIEEVRANENWRFI
                     LTTREYILNAARLRYEAFAHPEVDLNLCVVKLDDYTRPIRARILYNHIYFSDLPKAHK
                     LALIRNKGYEGIILHRNYNPRVIEYITEVRHACTVQASLYLHEFTDSLNHPTRIWDHA
                     FRHQISEAARHLLLVLSTMPDEILIDDLEDAFWAFYRFRQTRFGFPTKSGDWNDALKE
                     LDGNFILTTKVGTDIAITFHNPSIRDFLEGFLSNSESDVLDLVVGAHFYDQYVTLWEG
                     KGGHRYRGIDHNCEEFLRHLRAHIFNQSCRTIRSVNSSGEIIGLSRRTISNESRAGFV
                     ALVSKEVRSQSGKELIRFVANELEKLWNQGKADKEDLARLLPQLVENGFVHGCPVFLA
                     AKHCLTTNIEDIGDFRGAAEFITVYPDEVLPDMVEELKSKFLEFAVEYSEGWDDDPDL
                     LRQVAGDLEFVAEKIEVDVSNLTEKLETRAGEIEEGRLEPEPDDDRDDDEGWGKVADY
                     SDDVNDMFQDLQDELEA"
     gene            complement(5845..6141)
                     /locus_tag="VG272_02985"
     CDS             complement(5845..6141)
                     /locus_tag="VG272_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWF12674.1"
                     /translation="MIRVELPQHLRTLARVGGEVTVEVTPPVTLRSVLNALEIAYPVL
                     RGTIRDHDTMQRRAFLRFFVCSEDWSHESPDKPLPEEVASGKEPLLVIGAIAGG"
     gene            complement(6138..7325)
                     /locus_tag="VG272_02990"
     CDS             complement(6138..7325)
                     /locus_tag="VG272_02990"
                     /inference="COORDINATES: protein motif:HMM:NF014110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12675.1"
                     /translation="MSNVRVLVGTKKGAFILSSDGKREKWDVSGPHFAGWEIYHMKGS
                     PADPNRIYASQTSSWFGQIIQRSDDGGKTWHQPGSPAGEAPAAGGGPAQGVSNKFVYD
                     TSSEAGKPLTTHQWYDGTQHPWEFKRVWHLEPSLTEPDAVYAGIEDAALFRSADGGIT
                     WNELPGLRGSDTGPRWQPGAGGMCLHTVILDPSNPKRMYIAISAAGAFRTDDGGKSWK
                     PINRGLRSQYIPDPNAEVGHCVHHVAMHPKRPNTLFMQKHWDVMRSDDAGDNWTEISG
                     NLPTDFGFVIDVHAHEPETVYVVPIKSDSEHFPLEGKLRVYRSRSGGNEWEPLSKGLP
                     QSNCFVNVLRDAMAIDTLDSCGVYFGTTGGQVYASADAGNNWAPIVHDLPAVLSVEVQ
                     TLP"
     gene            complement(7440..7871)
                     /locus_tag="VG272_02995"
     CDS             complement(7440..7871)
                     /locus_tag="VG272_02995"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWF12676.1"
                     /translation="MSAASQPIMQPEITPGSTSKVSVWAGRIISGLFTAFMIFDGVIH
                     IMKPAPVVEAFAKLNFPLRFAVPLAIVELISLLLYVIPRTSILGAILLTGYLGGAVAI
                     QMPTGNSLFGEILFPVYFGVLLWGGIYLRDEKLRALIPFRR"
     gene            complement(7897..8322)
                     /locus_tag="VG272_03000"
     CDS             complement(7897..8322)
                     /locus_tag="VG272_03000"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWF12677.1"
                     /translation="MQLNAYLLFKGNCEEAFKFYERALGGKIEAMIPHAGTPAEKHVS
                     PEWRDKIMHARLAVGNAVLMGSDAPMDKFDEPKGFSVSIHTDDPAEAERLFQALEKDG
                     TVKMPLQQTFWAVKFGMLVDRFGIPWMVNCQQAAQQASS"
     gene            complement(8415..8831)
                     /locus_tag="VG272_03005"
     CDS             complement(8415..8831)
                     /locus_tag="VG272_03005"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWF12678.1"
                     /translation="MMIVKANKDSEAGKMPSEELLMAMGKYNEELVKAGVLVDLSGLQ
                     PSSKGFRVKFSGGKTTVIDGPFAETKELIAGYWVINVKSRGEALEWAKRIPAPFGKDQ
                     EGVIEVRQFFEMEDFAPSKAIDLHTEVGKELAKQKK"
     gene            complement(8936..9409)
                     /locus_tag="VG272_03010"
     CDS             complement(8936..9409)
                     /locus_tag="VG272_03010"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWF12679.1"
                     /translation="MQKITPFLWFDGKAEEAANFYVSIFKNSKIGKVNRYGDHGPGPK
                     GTVMIATFQIDGQDFIALNGGPQFKFSPAISFVVNCETQEEVDDLWEKLSEDGEKRQC
                     GWLQDKFGISWQIVPTALPKLMSDPDREKTGRVMQALMQMTKIDIKKLEDAFRGK"
     gene            complement(9424..9789)
                     /locus_tag="VG272_03015"
     CDS             complement(9424..9789)
                     /locus_tag="VG272_03015"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWF12680.1"
                     /translation="MRFLSVYKTVETGLPPTQEEMASMGKLIEEGMKAGYLLAVEGCL
                     PSAKGARVSQSNGKVVVTDGPFTETKELIGGFAILKADSKEHAIELTKHFLGIAGDGE
                     CELREVYEAGPMEQSSGRQ"
     gene            complement(9869..10225)
                     /locus_tag="VG272_03020"
     CDS             complement(9869..10225)
                     /locus_tag="VG272_03020"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWF12681.1"
                     /translation="MKYMLLIYLDENGLSERDRAQCYQDSAQFAVQLSKTGQYLDAGP
                     LHPTSTATSVRVRDNKRLVTDGPFAETREQLGGYFLIDAKDLDEAIGIAGRIPAGRWG
                     TVEIRPVMEISGLPNG"
     gene            10304..11566
                     /locus_tag="VG272_03025"
     CDS             10304..11566
                     /locus_tag="VG272_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWF12682.1"
                     /translation="MASTVSVTSAIEEVYRAEWGRIVATLIRLFSDFDLAEECAQEAF
                     AAAVGQWSEHGIPESPRAWIIQTAKHKAIDRIRRRGLYAEKLESYVVSGFIRAAEDPD
                     YDNDQISDDRLRLIFTCCHPALAPEAQIALTLRTLCGLETDEIARAFLVSPTTMAQRL
                     VRAKNKIREAGIPYIVPGTNDIAARLEAVLTVIYLVFNEGYVATRGASLVRTDLCSEA
                     IRLGRLIITLMGQQPPAEAKALLALMLLHDARRDARLDAAGEIVTLEDQDRSRWNHQQ
                     INDALLLLKDSSSGARGPFTLQAAIVAVHCQAARPEDTDWAEIVRLYNQLQRVQPSPI
                     VSLNRAVAIAMAQGPRAGLLAMNELAAADDLNDYHLLHAARADLLRRLGLHGEAAQNY
                     SRALELATNESERRFLERRLREVQQPSE"
     gene            complement(11595..13031)
                     /locus_tag="VG272_03030"
     CDS             complement(11595..13031)
                     /locus_tag="VG272_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWF12683.1"
                     /translation="MIKIRGMTRATFAITLLLCALFLSGCTVGPNYKRPNTDVPTGYR
                     VLPPTEPVVTPATASAQPLGDQKWWEVFQDDQLQKLIRTALQQNFDMRIAATRVLEAQ
                     AQLGITRANQYPNVRAVGTGTNLHNPAQGPFPSYGYSIGEITAAASWDVDFWGKYRRA
                     TEAARANLLATGWAQQEVNATLVANVAAAYFQLRELDLELDISQKALASRKESLDLTK
                     TLEEHGINSILDVRQAEQLVYTADAEITDLNRRIAQQENFLNVLLGNNPGPISPRGKD
                     LVDQPHPPLVPSGLPSALLERRPDIRQSEESLVAANAQIGVARAAYYPDISLTGSAGF
                     QSASLTNLFSGPTGLWNFAGNVTQPIFNAGQIRSNVRLAQAQQQEAVLTYQQTIQGAF
                     RDVSDALIAYQQNQQFRAQEELLANSAEDAAQLSNKRYEAGTTNYLEVLTNQTNYLSS
                     ELGLAQARLNELLSLVQIYKALGGGWQQ"
     gene            complement(13279..16446)
                     /locus_tag="VG272_03035"
     CDS             complement(13279..16446)
                     /locus_tag="VG272_03035"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWF12684.1"
                     /translation="MNLSEPFIRRPVMTAVLTISVIVFGVLSFLRLPVNDLPVVDYPV
                     ISVQANYPGASPDTVANNIATPLERQFMQINGLELVTSQSTQGHVSITLQFALDKSID
                     AAATDVQTAISQASGNLPTDLPSPPTFSKTNPNDQPILYIALTSDSVTPGRLYDYGAT
                     QVGQRISILPGVSSVNVFGTKSAVRIKVDPSKMWARGISIDDLVAAVKNGTNYTGAGQ
                     FDSPSGTSLLRPNGQLETAEDYGNLIVSSTNGAPVFLHDVANVENTVQDERIKMRFWV
                     RGYDEPSATVVVAVNRQAGSNAVAVSKSVRDLLPVISAELPGSVRVTPIYDRSQTIVN
                     SVADVQATLVFAFILVVGVIFIFLGRATDTLIPTVALPLSLLLTFMAMRVFGYSLDNL
                     SLMALTLAIGFLVDDAIVFLENTVRLMEKGMKPLEASLESAREISFTILSMTVSLAAV
                     FFPLVFMSGLVGRIFREFAITIVVSIIASGLVSLTLTPLMCARLLKPRGHGVKQNWVE
                     RTGGRVEKAVLGVYGKSLWWFLRHRWISALTWAACMAGTVLLFMAVPKSFLPVGDSSF
                     VWGVMIGKEGSSPEQMRALQNQAAIALRSDPAVGATFTLTGNSQFLASNQGLLLSFLK
                     PPQVRPPIQAVVGGMIRNLSSIPGIFAFLRPMPVLNISTGATNQNQGQYAYAISGVNS
                     QQVYDVAQKMMGRLRDYPGFATISSDFFNHTPNLEINIRRDQARIYGVSESRILNLLR
                     NAYSQNYLYLIKKPEDQYQVILEVQDSERAQADNLSLLYIRSDDGKNLVPLNTLVDWT
                     STLGPQSVNHLNQFTSVTIYFNLKPDVAIGDATNFIGKAEAEIVPPTIRASLQGEALT
                     FRDTVRDLTVLMGLAVFVMYVILAILYESYVHPLTVLSTLPTALVGGLLTLYVFGEQA
                     SLYAFVGMFMLMGIVKKNGIMIVDFARQRVDAGETAENAIHEASLDRFRPILMTTLAA
                     VIGAVPIAAGYGADGASRRPLGMVIVGGLVISQFITLYVTPVIYLYLEQFQEKVLNRT
                     SFFHSGQTQIPAAGAQIAAGDSTAD"
     gene            complement(16443..17576)
                     /locus_tag="VG272_03040"
     CDS             complement(16443..17576)
                     /locus_tag="VG272_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWF12685.1"
                     /translation="MLRWQQTFYLACALGILLTACAKKDPAPMERPPATVTVASAVSQ
                     DVPLYLDEVGHTVAREVVSVQPQVSGKIMEIHFADGANLKKGDLLFTIDPRPFEAQLN
                     EAQANLAQSKAALDFAQVQFARVKDLIESRAIARQDYDTRKNAVDVGQAQVKQNEAAV
                     ESARLNLEYTSIQSPIEGRAGHRLVDSGNVVTANTTTLLTIERLDPIYADFTATESDL
                     SLVQRELAKHPLKTEVRLPDQPKPETGQLTFVDNSVQSSSGTVMLRATVPNPARHLWP
                     GQFVNVRLVLANLPKAVLVPAAAAQDSAKGPFVYVVKEDSTAELRPVKLGQRQGEMVV
                     IAQGLQAGEHVVMNGQLGVMPGGKVRVVTNTPTAAQVAPAGQL"
     gene            complement(17971..18171)
                     /locus_tag="VG272_03045"
     CDS             complement(17971..18171)
                     /locus_tag="VG272_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12686.1"
                     /translation="MSDSLQESQKTQLIVRSPLSTEGFVSIYFSSPEGRLMSDEEWWN
                     LRQLIELTNKVCVKQQVRAQSA"
     gene            complement(18293..19870)
                     /locus_tag="VG272_03050"
     CDS             complement(18293..19870)
                     /locus_tag="VG272_03050"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12687.1"
                     /translation="MPSKYRTRFPLVFSIGAIGTALFLLSGLVSAQTPAKTSSAAAGT
                     AEQHTSRAYEGVRANPLELEAFLRRMPKGADLHYHESGGIYAESFIRAGAEDGLCVDL
                     EHHAFVAPAPTCGIGQVGAAEALTNQTLYDALVDSFSMRSFVPTTGTNGHDRFFDTFS
                     KFRGTTNRHSGEWLDEIASRAAAQNEQYLEIMETPDFGHTIRISREIGWQDDLAKLRD
                     ALLAAHLSDDIAVAKKDRDDAEASRQAIEHCGTLQATPACHVKIRYIYQILRDFPKQQ
                     VFAQTLLGFEVATADPRFVGLTYVQPEDNSTPMADYEWQMKFVEYLHRIYPKPHINLH
                     AGELAPGLVPPEGLCCHIRLAVEVAHSERIGHGVDVMHEDRPYDLLKEMAARHVMVEI
                     NLTSNDVILGVKGNEHPLPIYRKYHVPVALSTDDEGVSRINLTHEYVRAAETYALSYA
                     DLKQMVRTGLEHNFLPGASLWHEPDVFTRPASACASDSLGGAKPSPGCSNFLDSNPKA
                     QEQWELEKRFKEFEAGF"
     gene            complement(19954..20967)
                     /locus_tag="VG272_03055"
     CDS             complement(19954..20967)
                     /locus_tag="VG272_03055"
                     /inference="COORDINATES: protein motif:HMM:NF018249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside permease"
                     /protein_id="HWF12688.1"
                     /translation="MKRPRIVPIKVIKVKVVVVSMFEVGEDTGDIPGEYQLWVEREHL
                     DQILPLPAAYHHVRMNKDGVLGLLTGVATAKAAASVMALGLDPRFDLSKAYWVIAGIG
                     GGDPADVSLGSAVWANHVIDGDIAYEIDAREIPADWPTGFVPLRKGTPYEQPVKAQLD
                     GEAYTLNQDLVDWAYQLSKSVPLADNDEMRAYRMKYTGFPYAMKPPFVTRGDTMSGGT
                     FWHGKRMDKWANEWTKYYTGGEGNFMVAAMEDTGTMQALTFLGQAGRVDINRVLVLRT
                     VSNFDREAPGETPAETLKSMAGGNYPAFLPSVEAAERVGDKVVRDIVEHWSERESKLP
                     HAP"
     gene            22168..22953
                     /locus_tag="VG272_03060"
     CDS             22168..22953
                     /locus_tag="VG272_03060"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWF12689.1"
                     /translation="MVDRSEIHIEVIAQGSGPVIVILPSLGRGAEDYAVVAGLLAQEG
                     FRVLRPQPRGIGASTGPMANLTLHDFAADIAAVIEHEGQGPAIVVGHAYGHFVTRMLA
                     TDRPDLVRGAVLAAASAGKVPAGVNEPSVSPEVREAIEKSGNLSLPEADRLHYLQIAF
                     FAPGHDPHIWLTGWYPETEKAENVASRATPVDEWFACGTTRVLDLQAENDAVAPRKFA
                     GVLKAALGDRVTVEVIPNAGHALVPEQPKAMASAIDSFARTLK"
     gene            23215..23445
                     /locus_tag="VG272_03065"
     CDS             23215..23445
                     /locus_tag="VG272_03065"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HWF12690.1"
                     /translation="MLSMKDKKIAGVCGGIAEYLDVDPTIVRLIWVALSVVPGGFVGG
                     VLAYVLAWAIIPKSPAFVPAAVATSQASAKTS"
     gene            complement(23475..24881)
                     /locus_tag="VG272_03070"
     CDS             complement(23475..24881)
                     /locus_tag="VG272_03070"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HWF12691.1"
                     /translation="MLSFLCLLGLFLFRTFSVQAQEAPLDQQPIQTAQSPAENASSDD
                     VTTLFPHFESERIWLSGQVNLITQWHPAFRSPYQGPNSLSPEAQNASSRVLTLLTGLR
                     INNSSEILLDVQETGGHGIGEALGLAGFTNLDVVRNPTLSKAPYVARLMWRQIIPLGK
                     EKAPSERTPLSLFKELPVRRLEIRFGKFSLVDFFDVNSYGSDSNFQFMNWTLDNNGAY
                     DYAADTRGYTYAVMVEYDDRHWALRFAEALMPKVANGLNLDADLARARSENVELELHR
                     AFVPHRETVVRLLGYANHANMGIYSVAVANFLAGKTPKPEITAHPLQTTTKYGFGVNI
                     EQPLTDWLGLFSRWGWNEGQHESYAYTEVDETVQAGAAAQGTPWKRKLDRAGLAFISN
                     GISRDHQEYLAQGGISFLLGDGTLNYGRENIVETYYTTHVWRGIFASFDLQHINNPGY
                     NRDRGPLLAPSLRLHVEF"
     gene            complement(25412..26701)
                     /locus_tag="VG272_03075"
     CDS             complement(25412..26701)
                     /locus_tag="VG272_03075"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HWF12692.1"
                     /translation="MRRTNRHIVMVVCAASFLCASSWAQLPKVPKQKPGGLQGQVVTA
                     KGVAVADAQILWQVADGETPHVLHSDAHGQFHIEPLRTGFYDLRASANGTWSEWEHNI
                     LVRPGADTNVTLWLAFKPPVAPVGLELKGAMRVWDAPVSGSVPRDSAIDANGNAWFTL
                     DQTGHIARFNPNSNEWKLFKIPTADSGPIGLVFDNQGDIWFAENNAGKIGHMDPKTGA
                     ISEFTPPTVKDPHSIVIGPDGAVWFTAENSNAIGRLDTHSGIITEFGVPTQNAHPYGI
                     AAADDHALWFCELSGGKLGRMDPATGTMAEYAPADPDVKPRRLVAVGGAIYFTDFGGG
                     RLGRITLGDKKFKFWDSPSGIHSAPDGIGADTAGKIWYEESGKDANTLVRFDPAVEVF
                     RTYPMPAANSAASGMARDAKGHLWVPLSGANKIAIIE"
     gene            complement(26751..27695)
                     /locus_tag="VG272_03080"
     CDS             complement(26751..27695)
                     /locus_tag="VG272_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12693.1"
                     /translation="MIQRFHIAALGICLFAGVSWISVSHAQALVSGTGTNISPNPAPS
                     ADQIHALIMRAIQNQHRDDLALQEFERVEHKIARSGENAGVTVDITERILPSTTGNIK
                     LKLAERGVPVTPQEYRNELQIAENIFSLAIHPNDHYKEDLAKYERRRRDRAELVDTAM
                     KAFRITWAGREMRTDSNGPHTFMKFLLNPDPDYEPINRFAASFQHVHAVLWVDEAQAQ
                     FARLEGDIATDIPFAGGIAGKVYRGGHVVMEQEEIAPGIWLPTVYTYDVDGRKFLFAF
                     GIHERTEITHYRRIGPPSQAIEVVRNDLNNLPAVTPTH"
     gene            27935..29230
                     /locus_tag="VG272_03085"
     CDS             27935..29230
                     /locus_tag="VG272_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWF12694.1"
                     /translation="MSFAKDMQAARKFVRRIYCVRSLLVLGAILLGARGSYAQALTPA
                     WVELGDEGKAFARIVVNTPQDCPAIQIDGVNKPMSLRPNMPDGLRPACEFAIPAGAKS
                     ASVNSQSLVLPKNNTTEIITIGDTGCRIKGRQIQACNDPALWPLREVASSAAAEKPNL
                     IIHVGDYLYRESQCPEASQAQCGGSPAGDNWDAWNADFFEPAAKLLSAAPWAFTRGNH
                     EDCNRAWRGWFYYLDPRPWSGKCEEYSAPYIVKLGNFQLAMLDSAWINENIVDERELQ
                     ITVAQLSSLQAKDAWLVTHHPFWGFYTDQRSGLPKPTTPVLEEAWEKAEPKNFNLILS
                     GHVHLFEYVSVDNRRPPQIVAGDGGTQMDVPIEISVKGTTIRGATVNGSRIREQFGYT
                     MLTKEGKYWHLELKNRGAKVLVTCTVPESSESCQSAGTD"
     gene            29612..32065
                     /locus_tag="VG272_03090"
     CDS             29612..32065
                     /locus_tag="VG272_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012975.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HWF12695.1"
                     /translation="MRGEIIEYPNRRIGLPKGNQGAQFAESPRKGSQQRHGAEQRQET
                     RSESRPVRPEANQMTGRLVAHRDGYGFVVPDTPRKDLEGDLFIGRDGIGDAMHGDHVL
                     ASIERRKRFGDGAGPARAEGRILRVIGRAHTTVVGLFRYGLRGNTVAPYESRLLQEII
                     IPPGDELTPELQGKLGAHPPKRGVRLPELDGAVVNVEMTRFPRGGVAPAGRVIEILGR
                     PGEFGVDVEILIRKHHLPHEFSLEVLAEAAAGAHPVTEAEREGRRDFRDLPIVTIDGE
                     TARDFDDAVYAKQLENGNWQLQVHIADVSHYVRRGSALDREARLRGTSVYFPNRAVPM
                     LPEELSNGICSLNPREDRLVMSAVMELDANGEIVASEFARGVIRSAERMTYTAVNAVL
                     QNDAAATAQYAHLAGHFSAMRDLAMILNARRARLGSIDFDLPEPVIEFNDAGQMIGVV
                     RSERNIAHRLIEEFMLTANQAVASYLESRGVGSLHRVHEKPDPKKILEFEAMAQTFGY
                     SLGIENLAERRMEVKHGSSRQQKFDRGARHGRRGGGREKPMTVSLPGVTDIAIRPSHY
                     QRLAEKIAGKPEERIVSFLMLRSLKQARYAADALGHFALAFDQYTHFTSPIRRYPDLI
                     VHRVLKWALDHPETKPAPKPASYGSPSKDDSTFGPYRRGELEAIAVETSETERRAETA
                     ERELMAWKTAQFMEQHLGEEYEALIISVQKFGFFVELTELFVEGMVPIDRIQELTGEH
                     VFYREQDHAIVSGSGHGQRTASRGGHEAGRDKNAKKKSGRGVSRSDQSGHVWKLGDRI
                     TVRAERIDPIRRRVEFSIV"
     gene            32175..32420
                     /locus_tag="VG272_03095"
     CDS             32175..32420
                     /locus_tag="VG272_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HWF12696.1"
                     /translation="MDSIGAFEAKTHLAELLDRVARGEKITITRHGVPAALLVPVEEK
                     EARMTHREIVEGMHALRKKVKRSKTSVRDMVEEGRRF"
     gene            32417..32833
                     /locus_tag="VG272_03100"
     CDS             32417..32833
                     /locus_tag="VG272_03100"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWF12697.1"
                     /translation="MKGIVIDASVALAWCFPDEASDYAESVLLALEKRAAMVPAIWAS
                     EITNALLVGERRNRLGRPEVRRFVELLKGLEIVQDVQTVTDTMNHVFPLAQEYRLSAY
                     DAAYLDVAVRRQIPLATLDSDLRKAALAAGMETFNA"
     gene            complement(32921..33283)
                     /locus_tag="VG272_03105"
     CDS             complement(32921..33283)
                     /locus_tag="VG272_03105"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWF12698.1"
                     /translation="MANPFVHIELNTTDVSKAKSFYGSLFNWTLEDVPMGAETYTMIK
                     VGEGTGGGIMKHPMPGAPSMWLAYVHVDDVAAATKKARSLGANVVKDVTEIMGVGSFS
                     ILIDPTGATLALFQPKAK"
     gene            33695..34048
                     /locus_tag="VG272_03110"
     CDS             33695..34048
                     /locus_tag="VG272_03110"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWF12699.1"
                     /translation="MANFLLLLHQTPTSFRNLSPDEIQKILGSYIAWRDGLVKRNKMR
                     GGEKLTDDGGRHLRTQSGKVSVTDGPYSESQEILGGFFMIEAANYDEAVAIAQTCPHL
                     VDGKWIEIRQVDAAH"
     gene            34285..35513
                     /locus_tag="VG272_03115"
                     /pseudo
     CDS             34285..35513
                     /locus_tag="VG272_03115"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
     gene            complement(35576..35968)
                     /locus_tag="VG272_03120"
     CDS             complement(35576..35968)
                     /locus_tag="VG272_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12700.1"
                     /translation="MATILSSPANSGAAEKTFTIMGANNENKNPDHFWGDGSCTALCF
                     SVSTTQASPKSTATAAVSATSPQPAAAPAPLPEPHPEIREAIESLRRARGHLEHAAHD
                     FGGHRVEAIKTIDESLRQLQECLKYDKD"
BASE COUNT         8318 a         9945 c         9880 g         8353 t
ORIGIN      
        1 ttgcgccgct ggtagacgag cgcggcgact tgccgccctt caaggtaatc gaggcggccc
       61 ccaaccagcg gaaaaccatc gctcgccagg tcttccaccg gaggcgagaa atcaagcttc
      121 ccatcgaacc acggtttcac cgtgtgttga tccgtagaaa ctacatccat cagatggttg
      181 gcttcgagcg agcgcacgtg gctcgtaaaa acttccgccg cgagttgttg ctcattagta
      241 gggcgatgaa aaccgggcag caattgccac gccacgacgc tgcacagagc aagcaccgcg
      301 aacgcgctgg cccagcgcaa aaattgaaac ggactccatt gacccgcgcg tttgggaact
      361 tttgtttgaa ctgaagctgg cgttggagtt ggggccggcg cggctacggc tgcaagcagc
      421 gaagtttgaa cgcgctcgcg caatccttcc ggcgccccga aggcgagcga tccacgttgc
      481 aacgccgcac gcaacgcctg ctgcctctca agttcccgtg cgcattccgc gcaaacctgc
      541 aaatgagttt ccatctcgat ggtgcgtgaa agatcaagct caccatccag atagccggga
      601 acaaagcgtt ttgccagctt gcaattcact tcataactcc ttgccaagag cggcgctcag
      661 cgattgccgc aatctggtcc gcgcccgcgc gagtctcgac atgaccgtcc cgatggaaac
      721 ggaggcaacg tcggcgattt ccttgtagga cattccttcg atctcccgca gcaccagaac
      781 ttcccgaaac atttccggca attccccgag tgcgcactgc aatgtggctt tgtcggcatt
      841 cgccaaaagc tgcagctcag ggtcggcctc tccagaaaat tcttcgctgt gaatttcctc
      901 gtcaaattcc acggccggtt ccgcaggccg gttctgatgc agccacgtgt agcaggtatt
      961 gcgcacgatg gtcagcagcc acgcccgcat atttccgccc tggtagccgt caaaaaagcg
     1021 gaaggcgcgc aaaaatgatt cctgcgtcac atcctgcgcg tcctcatcgt ttcgcgtcag
     1081 ccagcgcgcg aggttgtacg ccgcgtccag atgaggcagg acggcttgct caaatcgctc
     1141 gcgtgctgct gtgccttgca ctcctgtgcg tcctgctttt cctattcgct cacttgtaag
     1201 actgccgccg ccactatttt attcccggcc ctgaaatcga caatttgccg ggaataagag
     1261 cggccaagcc cggtcttaca tcacgaaggc cgcgtgcggc aagtttttca taagccgcat
     1321 tcgcggaagg aggaacgata acatggctga cttcattcga gacaatatca acaaagacgg
     1381 catcgaccgc cgggggtttc tgcaatgcat ggcctgggcg ggcacgggcg tgctctattc
     1441 cgtccacggc ggaattttga aatctctgcc cctgatgcag ggcgccgcaa acgcgtacga
     1501 catggcaaag atgaagggcg cggatttcag cttcatccag atcagcgact cgcacatcgg
     1561 tttcagcaag gaagctaacc ctgacgtcac cgcgactctg caagttgccg tcgatcgcat
     1621 caacagcgcc gcggcaccac ccgattttct gctacacaca ggagacattt cgcagctctc
     1681 caaaccgtct gagttcgaca ccgccgagca ggtactcaag ggcgcgaagg cgagcaagat
     1741 tttttacgtt cccggcgagc acgatgttct caccgataac ggccagcaat acctcgatcg
     1801 cttcggcaaa gatacaaaag gaaacggctg gtacagcttc gatcacaagg gcgcccactt
     1861 catcggcctc gttaacgtaa tggacctcaa agctggcggc cttggctcgc tcggctccga
     1921 tcagctcaag tggctgcagg acgacgtgcg aaatttaaaa agcagcacgc ctgtaattgt
     1981 gttcgcgcac attccgctct ggacggttta tcccgaatgg ggatggggca ccgacgacag
     2041 cgagcaggcc ctcggatact taaaacgttt cggctcggtc tccgtgctga acggccacat
     2101 tcaccagatc atgcaaaagg tggaaggcaa cgtcacgttc cacacggcgc tctccaccgc
     2161 cttcccgcaa cctgcgccgg gcacagctct gtcacccggc cccatgaaag tgcccgccga
     2221 ccaattgcgc agcgtgttgg gcctttcgca tgttaactac gtgcaaggca accacgcgct
     2281 ggcgcttgtc gattcaacgc tggcgtaatt ccgaaaaaat ctcagattcc agaaaaattt
     2341 cagaaggaga agaatgatga aaagtatcaa attgatttgt gcaatggcgg caatcctgac
     2401 tacggcgctt gcattaggcg ccgcggccag gcccggtttg gcggggaacg agaacgacaa
     2461 acccgcaagc gcggccgaaa taaaaatcga caacttcagt ttcggcccaa caaccctcac
     2521 tgtgcccgca gggacgacga tcacctggaa gaacaatgac gacgttcccc atgtggtaag
     2581 cagcgatgac ggcaagatgt tcaaatcgaa ggcgctcgac accgacgatc gcttctcgtt
     2641 caccttcaca aaaccaggca cctacaacta ctactgcgcc attcatccga agatgaccgc
     2701 caaaatcgtg gtgcagtaat gcgaggagat tccgatgaat ccgtgaaatc agtgctgctc
     2761 gctaaacatg cgcagcatgt ggccctcgtc cactttccca tcgcgctgat ctttgtcagc
     2821 gccgcgttcg atctgctttc gttgcgcaag gaaaatctgg cactgcttcg agccgcctac
     2881 tacacggtca tcgcggcggc gatcgcagca gtgccaaccg tcgcaaccgg catcctcgcg
     2941 tggcaattcc aatttgaagg aaaagtcccg cggggaaatc tcaagctgca tatgttcctg
     3001 gcgttttttt ccagtgtcct gatctggctg gtttgcgggt tacacgttcg cgaacatcgc
     3061 aaactagagg ccggttcacc gaatctccga ttagccgttg aattcctggc cgttgctcta
     3121 actctattga ccgcgcacgt cggcggtttc gtcagtggag tcaacggtcc ggcgtaggcc
     3181 ttgaaatcgt cgacctgtcg ttgagattgt caaaatccgt caacgttcca tccagggcag
     3241 tggccattca aagtgggcaa aggtcgcgac actctatctc tgtgcatgct catgctaccc
     3301 atatgagaga ttacttgaat gtccgaatct tcgaagctgt tacaatccgg tctcgtccac
     3361 catcccttct agtctcttcc ccttcctcat tcaacactct gtaaaacacc tttcggagct
     3421 cgtgatatta tttaattcca actatgccaa attatgattt tactgcactg tctagttatg
     3481 aattcgaaat attggccaga gacctacttc aagcagagag cgggattcga cttgaatcat
     3541 tttctgctgg tccagactcc ggaatagact ttcggtaccg aaacggaaat attaatctaa
     3601 ttgttcaatg caaacactat gctgaatcag gatttcctgt tctatgtagt gttttacgtc
     3661 ggaaagaacg aaaaaaaatc gatatccttt ctccaactcg atacgttctg gcgacatcag
     3721 tcccactcac cccaaacaat aaagacgaaa tcaaaaatat tgtgggaccc cactgtttgg
     3781 gaactaccga catatacggg cgcgaagact tgaataatct actcagtctg cactctgaaa
     3841 ttgaggggaa gcattttaaa ctgtggttaa cgagcgaacc ggtacttcta cgagtattga
     3901 atgcaggaat cttccatgac tcacaggctc atcttgagcg cattcgtctg cgattacgtc
     3961 gatatgtcca aaacccaagt tttaaacgcg cacgctctgt cctagaacgc tcgcattatt
     4021 gcataatcgc ggggattcct gggattggca aaacaacgct tgccgaagtt cttctcgcag
     4081 atcttgtgga ccgcaaggga ttcgagacct tccgcatttc ccgtggactg gacgagatcg
     4141 gtccaattaa aaacaccaaa cgcaagcagg cgttttactt cgatgatttt cttggaacca
     4201 cttctgtgga taaactacag aagaacgagg accgaagatt aatcgatcta atcgaagaag
     4261 tacgagccaa tgagaattgg agatttatac taacgacccg agagtatatc ttaaacgcgg
     4321 cgcgtctccg ttacgaagct tttgcccatc ctgaagtcga tctaaactta tgtgtcgtca
     4381 agcttgacga ctatactcgc ccaatacgag cacgcattct ttataaccac atatattttt
     4441 cagaccttcc caaagcccac aaacttgcgc tgatccgcaa caaagggtat gaaggaatta
     4501 ttctgcatcg caattataat cctcgtgtca tcgagtatat aactgaggtt cgacatgcat
     4561 gtaccgtaca agcttccttg tacttacatg agtttaccga cagtctaaac catcctacaa
     4621 ggatttggga tcacgcgttt cgtcaccaaa tatcggaggc agcgaggcac ttattgctcg
     4681 tgcttagtac aatgcctgac gaaatcttga tcgatgacct ggaagatgca ttttgggcct
     4741 tttaccggtt tcgtcagacg cgattcggtt ttcccacaaa aagcggagat tggaatgatg
     4801 cgttaaagga acttgatggg aatttcattc taactactaa agtgggcaca gacatcgcca
     4861 tcacattcca caatccttca atccgggatt ttctggaagg gtttctatca aattcggagt
     4921 ctgatgtact tgacttagtc gtgggagcac acttttacga tcagtatgtg actttatggg
     4981 aagggaaggg tggacacaga tatcgtggca ttgatcataa ctgcgaagag tttctacgcc
     5041 atctccgcgc ccatattttt aatcagagtt gccgcactat taggtcggta aattcctctg
     5101 gcgaaattat tggactaagt cgtagaacga tttccaacga gagccgagcg ggatttgttg
     5161 ctctggtttc taaagaagtt aggagtcaat caggaaagga gcttattagg tttgtcgcca
     5221 acgaacttga aaaattgtgg aatcaaggaa aagcggacaa agaggacttg gcccgtttac
     5281 ttccgcaact agtcgaaaat ggattcgtac acgggtgccc agtttttctg gcagccaagc
     5341 actgcctaac tactaacatc gaagatatag gagactttcg aggtgcggcg gagtttatta
     5401 ctgtgtaccc agacgaagta ttgcccgaca tggttgaaga attgaagagc aagtttttag
     5461 agttcgcggt tgaatactcc gagggctggg atgacgatcc agacctgctg cgacaggttg
     5521 ctggagacct tgaatttgtc gcagagaaga ttgaggtaga cgtctcaaac ctaacggaga
     5581 aactcgaaac gcgggcaggt gagatagaag aagggcgcct cgaaccggaa ccagacgatg
     5641 atcgagatga cgatgagggc tgggggaaag tcgcggatta ctccgacgac gtgaatgaca
     5701 tgtttcagga tctccaagat gaacttgagg cctaagcagt tattttcaaa cggaatcaga
     5761 cgatcactac attcgaaatt gctttcaaaa taagtttgag tggttcagct atgcaatctc
     5821 gcatccgttc ttcaacttcg ctcctcaccc tcccgcaatt gccccaataa ccagaagcgg
     5881 ctccttgccg gaagcgactt cctcgggcaa aggcttgtcg ggagattcgt gggaccaatc
     5941 ttcggagcag acgaagaagc gaaggaaggc gcggcgctgc attgtatcgt ggtcgcggat
     6001 ggtgccgcgc agcacgggat atgcaatttc gagcgcattg aggacggagc gcagcgtcac
     6061 gggaggggtg acctcgacgg tcacctcgcc cccgacgcgc gctagcgttc gcaagtgttg
     6121 cggcagttcc acacggatca tggcagcgtt tggacctcta cggagagcac ggctgggaga
     6181 tcgtgaacaa tgggtgccca attatttcca gcgtcggcgg aggcgtacac ctgcccgccg
     6241 gtcgtgccga aatatacgcc gcaggagtcg agggtatcaa tggccatggc atcgcgcaaa
     6301 acgtttacga agcaattgct ttgcgggagg cctttgctca gcggctccca ctcatttccg
     6361 cctgagcggc tgcgatacac gcgcaatttt ccttccaacg gaaaatgttc ggagtcactc
     6421 ttgatgggaa ccacgtagac ggtttccggt tcgtgcgcgt gcacatcgat cacgaatccg
     6481 aaatcggtgg gcaggttgcc gctgatctca gtccagttgt cgcccgcatc gtcgctgcgc
     6541 atcacgtccc agtgtttctg catgaagagt gtgttgggcc gcttgggatg catggccaca
     6601 tggtgaacgc aatgtcccac ctccgcattc gggtccggaa tgtattgcga tctcagcccg
     6661 cgattgatcg gcttccaact ttttccgccg tcatcggtgc gaaacgcgcc cgccgccgaa
     6721 atggcgatat acattctctt cggattgctg ggatcgagaa tcacagtatg caggcacatg
     6781 ccgcccgctc cgggctgcca gcgcgggccg gtgtcactgc cgcgaagtcc gggcagctca
     6841 ttccaggtga tcccgccatc agcggagcgg aacagcgccg cgtcctcgat tccggcgtag
     6901 accgcgtcgg gctcggtcag cgacggctcg agatgccaga cgcgcttgaa ttcccaggga
     6961 tgttgcgtgc cgtcatacca ctggtgtgtg gtcagcggtt tcccagcctc gctcgacgtg
     7021 tcgtagacaa atttattgct cacaccctga gccggccctc caccggcagc cggcgcttcg
     7081 cccgcgggcg agcccggttg atgccaagtt tttccgccgt catcggagcg ctgaatgatt
     7141 tgcccgaacc agctcgaagt ttgcgacgcg taaatccgat tcggatccgc gggagatccc
     7201 ttcatgtgat aaatctccca gccggcaaag tgtggcccgc tgacgtccca cttttcgcgc
     7261 ttgccgtccg aagaaagaat aaacgcaccc ttctttgtcc ccacgagtac acgcacgttg
     7321 ctcattgttt cgttccctcc tgaatttcgt atatcgatct cgtatccctg cgtggcaagt
     7381 tttttagctt ggttctttta ttcaggcgtc gatcaacatg ttttccattg tgtcccaggt
     7441 cacctgcgaa aaggaatgag agcccgcagc ttctcatcgc gcaagtaaat ccctccccag
     7501 agaaggacgc caaaatatac cggaaacaaa atctctccga aaagggaatt tccagttggc
     7561 atctggatgg caaccgcacc gcccagatag cccgttaata gaatcgcgcc aagtatcgaa
     7621 gttcgcggaa ttacgtaaag cagtagggaa ataagttcca cgattgcgag agggaccgcg
     7681 aagcgcaacg gaaaattgag cttcgcgaat gcctccacca ccggcgcggg cttcatgata
     7741 tgaatcactc cgtcgaaaat catgaaggca gtgaacaaac cgctgataat tcttcccgcc
     7801 caaacggata ccttcgacgt cgaaccgggc gtaatctcag gttgcatgat cggttgagag
     7861 gctgcagaca taaaatatat ttctccttgg tgcgatttac gaggaagcct gctgtgcagc
     7921 ttgctggcaa ttcaccatcc acggaattcc gaagcggtcg accagcatgc cgaatttcac
     7981 ggcccagaaa gtttgttgca acggcatctt cacggttccg tccttttcca gtgcttgaaa
     8041 tagccgctcc gcttcggcgg gatcatctgt atgtatcgaa acagaaaatc ctttgggttc
     8101 gtcgaacttg tccatcggcg cgtcgctgcc catcaaaacc gcattgccca ccgcgagacg
     8161 ggcgtgcatg attttatcgc gccattccgg cgatacatgc ttttcggctg gagtgccggc
     8221 atggggaatc atcgcttcga tttttccgcc aagtgcgcgc tcataaaact taaacgcctc
     8281 ttcgcaattc cctttgaata gcaaatacgc attgagttgc atgacgatct ccttccagga
     8341 ttttgagatt gaaaatcaat tccccttcac tgattaaacg aatggcaaac cgcgaaatcg
     8401 acagccaccg gcaattattt tttctgcttg gccagttctt tccccacttc cgtgtggagg
     8461 tcaatcgcct tgctcggcgc aaaatcctcc atctcgaaaa attggcgaac ttcaatcacg
     8521 ccttcctgat ccttgccgaa tggagcaggg atgcgcttgg cccattcgag agcctccccc
     8581 cgggatttca cgttgatgac ccagtagccc gcgatcaatt ctttcgtttc ggcaaacggc
     8641 ccgtcaatca cggtcgtttt tcctcccgag aatttgactc gaaacccctt cgagctcggc
     8701 tgcagccccg atagatcgac cagcacgccg gccttcacca actcctcgtt atattttccc
     8761 attgccatga ggagttcctc gctgggcatc ttgcccgcct cgctatcctt attcgctttt
     8821 acaatcatca tgaatcgcat gtctatctcc tgttttttaa gtagcacagg cttcagcctg
     8881 tggggtttag tccttgcaag atcaaaattc cacaggctga agcctgtact accgatcact
     8941 tccctcgaaa cgcatcttcc aatttcttga tatcgatctt ggtcatctgc atcaacgcct
     9001 gcatcacccg acccgttttt tcgcggtcgg gatcgctcat caacttcgga agagccgttg
     9061 gaacgatttg ccacgaaatc ccgaatttgt cctgcagcca accgcactgc cgtttctctc
     9121 cgtcctcgga cagcttttcc cacagatcat ctacttcttc ctgcgtttcg caattcacca
     9181 cgaacgaaat ggccggagaa aacttgaatt gcggtcctcc gttcaacgcg atgaagtcct
     9241 gcccatcgat ttgaaacgtg gcgatcatga cggttccctt tggccccggc ccgtggtcgc
     9301 cgtaacgatt gaccttccca atttttgaat ttttgaaaat ggaaacataa aaattggccg
     9361 cttcctcagc ctttccgtcg aaccacaaaa acggcgtaat tttttgcatg gatttattct
     9421 cctttactgc cgcccgctgc tttgttccat tggtccggct tcgtacacct cgcgcagctc
     9481 gcattctccg tcgcctgcaa ttcccagaaa atgttttgtc agttcgatgg cgtgctcctt
     9541 ggaatcggct ttcaggatcg cgaagcctcc aattaattcc ttggtttcgg tgaaggggcc
     9601 atcggtcacc acgactttgc cgttcgactg gctgacccgc gcgcccttcg cgctgggcaa
     9661 acaaccttcc acggcaagca gataaccggc cttcatccct tcttcgatca gcttgcccat
     9721 gctggccatt tcctcttgcg tggggggaag ccctgtttcc acggttttgt aaacgcttag
     9781 aaatcgcaat ttgattctcc ttaatttatt taggttgagt tttttatttc ttctgagacg
     9841 aatgaatgta attcccatcc taagttcact acccattcgg cagtccggaa atctccatca
     9901 cgggccgaat ctccaccgtt ccccagcgcc ccgcgggaat ccgcccagcg atcccgatgg
     9961 cttcatccag atctttcgcg tcaattaaaa agtacccgcc cagctgctca cgcgtttcgg
    10021 cgaaagggcc atccgtcact agccgcttgt tgtcgcgaac gcggacgctc gtcgcggttg
    10081 aagtggggtg cagcggcccg gcgtccaaat attgcccggt cttgctgagc tggacggcga
    10141 attgtgccga gtcctgatag cactgcgccc gatcgcgttc gctcaaccca ttctcatcca
    10201 aataaatcag cagcatgtat ttcatttctc tctccctctg cttataagtc gaatagcgca
    10261 ggcacagatc gacatctata tcaaaaaatt cgtacaatcg gccatggctt ctaccgtcag
    10321 cgtaacctca gcgattgaag aagtttaccg tgccgaatgg ggtcggatcg tcgccaccct
    10381 gattcgcctt tttagtgatt tcgacctggc cgaagaatgc gcccaggaag cctttgccgc
    10441 cgccgtgggc cagtggagcg aacacggcat cccggagtct ccccgcgcgt ggatcattca
    10501 gacggccaaa cacaaagcta ttgaccgcat tcgccgccgg ggcctctacg ccgaaaaact
    10561 ggaatcttac gtagtatccg gcttcatccg cgccgccgag gacccggatt acgacaacga
    10621 tcaaatttcc gacgaccggc ttcggctcat cttcacctgc tgccatcccg cgcttgcccc
    10681 cgaagcgcag attgccctga ccctgcgcac gttgtgtggt ttggaaacgg atgagattgc
    10741 gcgggccttt ctcgtttctc ccacgacgat ggcgcagcgt ctggtccgcg ccaaaaacaa
    10801 gattcgcgag gcgggcatcc cgtacattgt ccccggaacg aatgacatcg cggcaaggct
    10861 ggaagcagtt ttaaccgtga tctatctggt tttcaatgaa ggttacgttg ctacccgtgg
    10921 cgcgtcgctg gtcagaaccg atctttgctc cgaagccatt cgcctggggc gcttgattat
    10981 tacgctgatg gggcagcaac ctcctgccga agccaaggca cttttagccc tcatgctctt
    11041 gcacgatgcg cggcgcgatg cgcgattgga cgctgcggga gaaatcgtaa ccttggaaga
    11101 ccaggaccgc agccgctgga atcaccaaca gatcaacgat gccttgcttc tattaaagga
    11161 ctcgtcgagc ggcgcacgcg gcccatttac tctccaagcc gcgatcgttg cggtgcattg
    11221 ccaagcggct cgtcctgagg acacggactg ggcggagatt gtccggctct acaatcaatt
    11281 gcagcgcgtc cagccctcac ccattgtttc attgaaccgc gccgtagcaa ttgcaatggc
    11341 gcagggacct cgtgcaggac ttttggcaat gaacgagtta gccgctgcgg acgatctcaa
    11401 tgactatcac ttgctgcatg ccgcgcgcgc cgatctcctg cgccgcctcg gcttgcatgg
    11461 cgaagcggcc caaaattact cgcgggccct cgaacttgcc accaacgaaa gcgagcgccg
    11521 attcctcgaa cgccggctcc gcgaagtgca gcagccttcc gagtaatggt gtgaaaaaat
    11581 aatggcggct gcagctactg ctgccagccg ccgcccagag ctttgtaaat ttggacgagc
    11641 gagagaagtt cattgagtcg agcctgtgcg aggccgagtt cagaggataa gtaattggtt
    11701 tggttggtca gaacttccag ataatttgtg gtgcccgctt cgtaacgttt gttggaaagt
    11761 tgtgcggcat cttccgcgga gttggcgagc agttcttcct gcgcgcggaa ttgctggttc
    11821 tgttgatagg caatcagcgc atccgaaaca tcgcggaacg cgccttgaat tgtttgttgg
    11881 taggtgagca cagcttcctg ctgctgcgcc tgcgccagcc gcacattcga acgaatttgt
    11941 ccggcattga aaatcggctg cgtaacgttc cccgcaaaat tccacaaccc cgtaggaccg
    12001 ctgaacaaat tcgtaagcga agcactttga aagccggcgc ttcccgtcag cgaaatatcc
    12061 gggtagtacg ccgctctcgc cacgccaatt tgggcattcg ccgccacgag tgattcttcc
    12121 gattgccgaa tgtccggccg ccgttccagc agcgccgatg gaagtccgga cggaacaagg
    12181 ggaggatgcg gctggtcgac aagatccttg ccgcgcggcg agatcggacc cggattgttc
    12241 cccaacaaaa cgttgaggaa attctcctgt tgcgcgatgc gccggttcag atccgtgatt
    12301 tccgcgtcag ccgtgtaaac caattgttcc gcctgacgca cgtccagaat ggaattgatg
    12361 ccatgctcct caagcgtttt ggtcagatcc agcgattctt tccgcgaggc cagcgccttt
    12421 tgcgaaatgt cgagttcgag gtcaagttcg cgaagttgaa aatacgcggc ggccacattc
    12481 gcgacgagtg tcgcgttcac ttcctgctgt gcccaaccgg ttgcgagtaa atttgcccgc
    12541 gccgcttccg tggcgcgccg atattttccc cagaaatcca catcccacga cgcggctgcc
    12601 gtgatttctc ctatggagta tccgtaagag ggaaatggtc cctgcgccgg attgtgaaga
    12661 ttggtgccgg taccgacagc ccgaacgttc gggtattgat tcgcgcgcgt aattcccagt
    12721 tgcgcctgcg cctcaagaac tcgagttgcc gcaatgcgca tatcgaaatt ttgttgcagc
    12781 gcggtgcgaa tcagtttttg cagctggtca tcttgaaaaa cttcccacca tttttggtct
    12841 cccaggggtt gcgcggaggc ggtagcagga gtgacgacgg gttccgtagg tggcagaaca
    12901 cgatagccag tcggcacatc cgtgttcggc cttttgtaat taggccccac cgtgcagccg
    12961 ctaagaaaaa gcgcgcacaa caaaagtgtt attgcgaaag tagcgcgcgt cattccacga
    13021 atcttaatca tcgaattcca gtgctccgtt ttagtgactt ttagcggcgc gggcttcggc
    13081 ctgcgtggtt tagtccatgc aagccctaaa ttccgcaggc tgaagagtct gtgtgagaac
    13141 tgcggaatct ttgtgggtcg tggccttagc cacgacataa gtcctgcaaa aggaatgcgg
    13201 ctttagccgc tgaagttcca attttgcagt tctcacacag actctgaagc ccgcgctacc
    13261 aaaatccaaa actaaatctc aatccgccgt gctatcccca gcagcaatct gcgcgccagc
    13321 agcagggatc tgagtttgtc ctgaatgaaa gaacgaagtg cgattcaaaa ccttttcctg
    13381 gaactgttcg aggtacaaat aaatcaccgg cgtcacgtat aacgtaatga actgcgatat
    13441 caccagtccg ccgacaatca ccatgccgag cggacgccgc gaagctccat ccgctccgta
    13501 ccctgctgcg ataggcaccg cgccaatcac agcggcaagc gtggtcatca agatggggcg
    13561 gaatcggtcc aggctcgctt cgtggatggc attttccgcg gtctcgcccg catcgacgcg
    13621 ctggcgcgca aagtcgacaa tcatgattcc gtttttcttc acgatgccca tcagcatgaa
    13681 catgccgacg aatgcgtaga gcgatgcctg ctcgccgaac acatacagcg tgagcaatcc
    13741 gccaacgagc gctgtgggca atgtcgagag cacggtcagc ggatggacat agctttcgta
    13801 caaaatggcg aggatcacgt acatgacgaa caccgcgagt cccatcagga cggttaaatc
    13861 ccgcaccgta tcgcggaacg tcagcgcctc tccctgcagg ctcgcgcgaa tcgtgggcgg
    13921 cacaatttcc gcttcggcct tgccgataaa gtttgtggcg tcgccgatgg ctacatccgg
    13981 cttcaagtta aaataaatcg taacgctcgt gaactgattc aggtgattca ccgattgcgg
    14041 ccccaaagtg ctcgtccagt ccaccagagt gttgagcgga acaagatttt ttccgtcatc
    14101 cgaccggata tagagcagcg acaaattgtc ggcttgcgcg cgctcggaat cctgtacttc
    14161 cagtatcacc tggtactggt cctccggctt cttgatcaga tacaaataat tttgcgaata
    14221 cgcgttgcgc agcagattca gaatccgcga ttccgacacg ccgtaaatgc gcgcctggtc
    14281 cctgcggata ttaatttcca gatttggcgt gtgattgaaa aaatccgaag agatggtcgc
    14341 gaagccggga tagtcccgca atctgcccat cattttttgc gcaacgtcat acacttgctg
    14401 cgaattcacg ccggaaatgg cgtacgcata ctggccctgg ttctggttcg tcgcacccgt
    14461 gctgatgttc aagaccggca tgggccgcag gaacgcaaaa attcccggta tcgagctgag
    14521 atttcgaatc attccgccaa cgacagcttg aatcggtggc ctcacttgcg gcggcttcag
    14581 gaatgacagc agcaatcctt ggttcgacgc cagaaattgg ctgttgcccg tcagcgtgaa
    14641 tgtggcgccg actgcgggat ccgaccgcaa ggcgatggcc gcctgattct gcagcgcgcg
    14701 catttgttcg ggcgaggaac cttccttgcc gatcatcacg ccccaaacga agctgctgtc
    14761 tcccactggc aaaaacgatt tcggcaccgc catgaaaagc aaaacggttc ccgccatgca
    14821 agcggcccac gtgagcgcgg aaatccagcg atgccgcaga aaccaccaca acgatttgcc
    14881 gtacacgcca agaacggctt tctcgactcg cccgccggtc ctctccaccc agttctgctt
    14941 gacgccgtgg cctctgggtt tcagcagcct cgcgcacatc aacggcgtaa gcgtcagcga
    15001 aacaagtccg gaggcaataa tcgaaacgac gatggtgatc gcaaactcgc gaaagatgcg
    15061 gcccacaagg ccggacatga acacgagcgg aaaaaatacc gccgcgagag aaaccgtcat
    15121 ggaaagaatc gtaaagctga tttcgcgcgc gctttcgagc gatgcctcga gcggcttcat
    15181 gcctttttcc atcagtcgca cggtgttttc caaaaacacg atcgcgtcgt ccaccagaaa
    15241 accgatggcc agtgtcagcg ccattaacga aagattgtcc aggctgtacc caaacacgcg
    15301 catcgccatg aacgtaagca agagagacaa gggaagagcc acggttggga tcagtgtgtc
    15361 ggtggcgcgg ccaaggaaaa taaaaatcac gccgaccact aaaataaatg cgaacactag
    15421 cgtcgcctga acatccgcga cggaattcac gattgtttgc gagcggtcgt aaatcggcgt
    15481 gacgcgcacc gagccgggga gctccgcgct gatcacaggc aaaagatcgc ggacgctttt
    15541 cgaaacggcc accgcattgg agcccgcttg gcggttaacg gcaacgacga ctgtcgcact
    15601 aggctcatcg taaccgcgaa cccagaatcg catcttgatg cgttcgtctt gcaccgtgtt
    15661 ttccacgttc gcaacatcgt gtagaaaaac gggcgctcca ttcgtgctgc tgacaatcag
    15721 attcccgtag tcttcggcgg tttccaactg gccgttaggg cgcaatagcg aggtgcctga
    15781 cgggctgtca aattgccccg cgccagtata atttgtcccg ttttttaccg cagcgacgag
    15841 atcatcaatc gaaattccgc gcgcccacat ctttgacgga tcaactttaa tgcgcacggc
    15901 ggacttcgtt ccaaacacat ttacgctgct tacgccgggc agaatgctga tgcgctgacc
    15961 cacttgcgtg gcgccgtaat cgtaaagtcg gccaggcgtg acggagtcgc tcgtcagagc
    16021 gatatacaaa atcggctggt cattcggatt tgtttttgaa aacgtcggag gcgaaggcaa
    16081 atcggtgggc aggttcccgc tggcctgcga aatggccgtt tgcacatcgg tcgcagccgc
    16141 atcgatgctt ttgtccaacg caaattgcag cgtgatgctc acgtgcccct gcgtgctttg
    16201 cgaggtcacc agttcgagcc cattgatctg catgaattgc cgctcgagag gagtggcaat
    16261 gttattcgcc acagtgtccg gacttgcccc aggataattt gcctgcacgg aaattacggg
    16321 gtagtccact accggcaaat cgttcaccgg aagccgcaga aaactgagca caccgaaaac
    16381 aatgactgaa atcgttaaga ccgcagtcat caccggacgt cgaataaatg gttcagagag
    16441 gttcataatt gtcctgctgg tgctacctga gccgccgtag gcgtattcgt cacgacgcgt
    16501 accttgccgc caggcattac acccaactgg ccattcatca ccacatgctc gccggcctgc
    16561 agtccctgcg cgatcactac catttcccct tgccgttgcc ccagctttac cggacgaagt
    16621 tctgccgtcg agtcctcttt aaccacgtac acaaatggac ccttcgccga atcttgcgct
    16681 gccgcggcag gcacaagcac agcctttggc agatttgcca gcaccagccg cacattgacg
    16741 aattgcccag gccacaagtg acgcgcagga ttgggaaccg tcgcgcgcaa cattaccgtt
    16801 ccgctgctgc tttgcactga attatcgacg aaggtgagtt gccctgtttc aggtttcggc
    16861 tgatccggca agcgcacttc tgttttgagg ggatgcttag ccaattcgcg ctgcacaagg
    16921 gagagatcgc tttctgtcgc cgtaaaatcg gcgtagatcg gatcgagtcg ctcaatcgtc
    16981 aacagcgttg tggtatttgc cgtcacgacg ttgccgctat ccacgagcct gtgtcccgcg
    17041 cggccttcga tcggcgactg aatcgatgtg tactccagat tcaggcgtgc ggattccacc
    17101 gcggcttcat tctgcttcac ctgcgcttgg ccaacatcca ccgcgttttt tcgcgtgtca
    17161 tagtcctgcc gcgcgatggc tcgcgattcg atcaggtctt tcacacgagc aaactgaacc
    17221 tgcgcaaaat ccaaagccgc tttcgattgt gcgaggttcg cttgcgcctc attcaattgc
    17281 gcttcaaatg gcctcgggtc aatcgtaaaa agcagatcac cttttttcag atttgcgccg
    17341 tccgcaaaat gaatttccat gatctttccg gaaacttgcg gctgcacgga cacgacttcg
    17401 cgcgccacgg tatggcccac ctcgtccagg tacagcggca catcctgtga caccgcggat
    17461 gcaaccgtca cggtggccgg cggccgttcc atcggagcgg gatctttttt cgcacacgcc
    17521 gtcagtaata ttcccaaagc gcaggccaga taaaatgttt gttgccagcg gagcatgaat
    17581 ctccttcgct ttctaaaacg ttagaagctg gactttatat actacgccac cctgaatcag
    17641 atgtttcaaa cggcaattgg gaccaaatta tttttgccct ggtttcccgg cgtcaagcta
    17701 aaggaaataa ctagagcgat tgaagaggag tggaaattaa ctcgacctgt tcataaaaac
    17761 atccaaaggc tgccagccca acacaggcaa ccttcacgac actgaatgtc aatccaaata
    17821 taataccgta aaaattcgtc cgcttcctcc cgggaggggt gaacggacat tcgtagcgtc
    17881 tgcttcaatt tttaatgcag cgtggaggca cgaaccattc gcgccagatt taaaatagcc
    17941 agatacgcta cacgtgcttg aaattcagta ttaagcagac tgagcgcgta cctgctgctt
    18001 cacacaaact ttgttggtca gttcgatgag ctgtcgcaaa ttccaccact cctcatcact
    18061 catcaatctg ccctcaggac tcgaaaaata aatggatacg aatccttcgg tcgagagcgg
    18121 agaacggaca atcaactgtg tcttttggct ttcctgcaat gaatcggaca tgggtaattc
    18181 ctccagatgg gcgcggattc taccacgaac cagtatctaa atctttaaca ttacgttaac
    18241 aacgaatggt cctcgaacat tcagctgaat cacgagttca acctgagcca gcttagaatc
    18301 ctgcttcaaa ttccttaaaa cgtttctcca actcccactg ctcctgcgcc tttggattgg
    18361 aatcgagaaa attcgagcag cccggggaag gttttgcgcc gccaagcgaa tcggacgcgc
    18421 acgcgctggc aggccgggta aatacgtccg gctcatgcca gaggctcgca ccgggcagaa
    18481 aattgtgctc cagcccggtg cgcaccattt gcttcaaatc cgcgtagctg agcgcgtagg
    18541 tttccgcggc gcgcacatat tcatgagtca aattgatgcg cgaaacgccc tcgtcatccg
    18601 tagaaagcgc caccgggaca tggtacttgc ggtaaatggg cagcgggtgc tcgttacctt
    18661 tgacaccgag aatgacgtca ttgcttgtga gattgatttc caccatcacg tgccttgcgg
    18721 ccatctcttt caaaagatcg tagggacgat cttcatgcat cacgtcaacg ccgtgcccaa
    18781 ttcgttctga atgagcaact tccacggcaa gtcggatatg gcagcacagg ccttctggcg
    18841 gtacaagccc aggcgcaagc tctccggcgt ggaggttgat atgcggcttg gggtatatgc
    18901 ggtgcaaata ctcgacgaat ttcatctgcc actcgtagtc cgccatggga gtcgaattat
    18961 cttcgggctg cacataggtg aggccaacaa aacgcgggtc ggcggtagcg acttcaaatc
    19021 ccagaagcgt ctgggcgaaa acctgctgtt tcgggaagtc gcgcagaatc tgatagatat
    19081 agcgaatttt cacatggcag gcaggggtcg cctgcagtgt accgcaatgt tcgatcgctt
    19141 gccgggaagc ctccgcatcg tcgcggtctt tctttgccac ggcaatgtca tcgctcaggt
    19201 gggccgcaag gagcgcgtcg cgcagcttgg caaggtcatc ctgccagccg atttcgcgtg
    19261 agatccggat cgtgtgacca aaatctggcg tctccatgat ttccaggtac tgttcattct
    19321 gagcggctgc gcgagacgct atttcgtcta accattcgcc ggagtgacga ttcgtcgtcc
    19381 ctctaaattt actgaaggta tcgaaaaagc ggtcgtgccc gttggtacct gttgtgggca
    19441 cgaaactgcg catcgaaaat gagtccacca gcgcgtcata caaagtttga ttcgtaagcg
    19501 cttccgcagc tcctacttgg cctatgccgc acgttggtgc gggcgctaca aaggcatggt
    19561 gttctaggtc cacacacaga ccatcttcgg ctccagcgcg tatgaaggat tcagcgtaaa
    19621 tgccgccgga ttcatgatag tgaaggtccg cacccttggg catgcgccgg agaaacgctt
    19681 cgagctccag cggattcgct cgcacgcctt cataggcgcg ggaagtgtgc tgctcggccg
    19741 tccccgcagc tgccgaactc gtttttgccg gcgtctgcgc agaaacaagg ccggacaaga
    19801 ggaaaagcgc tgtcccaata gctccaatag aaaagaccag cggaaaccgt gtacgatatt
    19861 tgctgggcac gttcttgtcc tttcgctaaa aatcgctaca actcggcggt gtctcagtgt
    19921 aggggcacga catgttgcgt cccaactgcc gcgctaagga gcgtgcggaa gtttcgattc
    19981 acgctcactc caatgttcca cgatatcgcg aacgacttta tcgccgacgc gctccgctgc
    20041 ctcgacagag ggaagaaatg caggataatt tcctccggcc atgctcttga gcgtttccgc
    20101 gggagtttcg ccgggcgcct cgcgatcaaa attactcacg gttcgcagca ccaatacgcg
    20161 attgatatcg acacgacccg cctggccgag aaacgtcagc gcctgcatcg tcccggtatc
    20221 ttccatcgcg gcgaccatga aattgccttc tccgcccgtg taatacttcg tccactcatt
    20281 ggcccactta tccattcttt tgccatgcca aaatgtgccg ccggacatgg tgtcgccgcg
    20341 cgtcacaaaa ggcggcttca tcgcataggg aaagccggtg tacttcattc gataggcgcg
    20401 catttcgtcg ttatccgcga gaggaacgct cttcgacaat tgatacgccc agtcgaccag
    20461 gtcctgattg agggtatagg cttcgccgtc cagttgcgcc tttactggct gctcataggg
    20521 tgtccctttt cgcaagggaa caaagccagt aggccagtcc gcgggaattt cgcgcgcgtc
    20581 gatttcgtag gcgatatcac catcgatcac gtggttcgcc cagaccgctg agcccagcga
    20641 tacgtccgcc ggatcgccgc caccgattcc agcaatcacc caatatgcct tggagagatc
    20701 gaagcgcgga tcgagcccca acgccattac ggacgccgcg gccttggccg ttgctacgcc
    20761 cgtcagcaga cccagcactc catccttgtt catgcgcacg tgatgatagg cagcggggag
    20821 cggaagaatc tgatccaaat gctcgcgctc aacccacaat tggtattcgc cgggaatatc
    20881 gcccgtatct tccccgactt caaacatgga tacgacaacg accttcactt tgattacttt
    20941 gatcggaaca attcgaggcc gcttcaccgg tttcctccgg gaataaagcg gagtgacgaa
    21001 gaaactgagt aagatgaaaa tccctatgga aaatttaaaa aagcgaccca catttctctc
    21061 ctggccgacg catttgctta ctccactcaa acaaggtgtc tgagtttaca tcagagcgag
    21121 ccgaggaaag tttccgaatg gattttaatt cgtgattgcc ggcgtaggga ttaacacgcg
    21181 gaagaaactc acgcacaaat actgaaattt taaaaaacgg tgaagtcgga caaaaccgaa
    21241 cagtctgcgg tcaggctttc gccgctcgcg tgagcccatc ctgcggatgc catcgcaatg
    21301 gtgccgggcc gtccaggggg gaaatagctt gaggagttcc ctcaaaatcg acaatttctc
    21361 ttgtgcgata gggcttctct ttatggcaag cggggcagcg aaggttaaat acttgcgatc
    21421 gccgttgccc atcttccgaa gactgcaagc tccctacaat agggggcact ggaatagacg
    21481 ccttgcaatg taggcaaacg acaccgcgaa atgtttgatt ttctgtattc gtcgtcatga
    21541 ttaaacctcg cagcgaagca atccgtgtac tggggacggg ttcatgctac tccattgagt
    21601 ctcgggccgt ccagtcggat aaaaggcaaa aactgtggaa atagcgcagc gggtgtttcg
    21661 gccttcaagt tatcagttaa gtattttgtt tgcagtagat tacattgatt tcttctaaag
    21721 ggctgtgcct tccctgtttt caatttccac agtttaatcc actcgggcca attgattccg
    21781 gcccagagag aacagccatg gcgaagagcc gaaggtgcga aaagtttcga aaaaaggtaa
    21841 ccggaaaaaa cgggagaggg gcaagaaatt gatcattcct cgaaatttca cgcggcacag
    21901 aggatgtccg aagtgaattt atccaaaaag cagcgccaat catgtatcct ttgaaaaaat
    21961 tctgtcgaaa agcgattttt aaaacaagaa atgactggac gcctgtaatc tgccatgaaa
    22021 ccgaagaaaa tgaaatgtac acaacggctg atttactttc tcggagcagt atattgcctg
    22081 actacctctc cgcagttggt acgtccgcag tccacttcag catcctcttc tcaaagccgg
    22141 gcgcatccgc cttcagaaca gcatttcatg gtggatcgga gcgaaattca catcgaagtg
    22201 attgcgcagg gaagcggccc agtcatcgtc atcctgccgt cgctggggcg gggcgccgaa
    22261 gattacgccg tcgtcgctgg gttgcttgcg caggaaggtt ttcgagttct gcggccgcaa
    22321 ccgcgcggca ttggcgcgag cacaggcccc atggcaaatt tgacgttgca cgatttcgcg
    22381 gcggatattg ccgccgtgat cgaacatgag ggacagggcc cagccatcgt agtcgggcat
    22441 gcttacggcc attttgtaac gcgcatgctg gccaccgacc ggcccgacct tgttcgagga
    22501 gcggtgctgg cggctgcgtc ggcgggaaaa gttcctgcgg gcgttaacga gccgtcggtt
    22561 tcccccgaag ttcgcgaagc catcgaaaaa agcggaaatt tgtctctgcc ggaagccgac
    22621 cgcctgcatt atttgcaaat tgcattcttt gcgccggggc acgatccgca tatctggctc
    22681 acgggctggt accctgaaac cgagaaggcg gaaaacgttg cctcgcgcgc cacgccggta
    22741 gacgagtggt tcgcctgcgg cacaacgcgc gtgctcgacc tgcaggcgga aaacgatgca
    22801 gtcgcgccgc gcaaatttgc tggtgtgtta aaggccgcgc tcggtgaccg cgtcaccgtc
    22861 gaagtgatcc ccaatgccgg gcacgctctc gttcctgagc aacccaaagc catggccagc
    22921 gccatcgact ccttcgcgcg aactttaaaa taaatttcct ttttgcatgg ggcatgaagg
    22981 tggttgcgcc gcgcgacttt ttaaaaaccg caagccgtgg atttggtttg cgtacggcgt
    23041 taaaatgaaa tcgcggagat tggcagctcg gaacaattgg aggaccccgg ttcgctgatt
    23101 tcaggaggtg accttatgta ttgcaatcga tgcagtgcgg aaattgctga cactgctaat
    23161 ttttgccctt cttgcggggc gcggcagcat tcgagaactt ctcaaaaaca gttgatgctt
    23221 tccatgaagg acaaaaaaat tgcaggagtt tgtggaggca tcgcggagta cctggacgtg
    23281 gaccccacaa tcgtgcggct gatttgggtg gcgctgtccg tagtgcctgg aggatttgtc
    23341 gggggcgtgc tggcttacgt tcttgcctgg gccatcattc ccaagtcgcc tgcgtttgtg
    23401 ccggcggcgg tagccacatc acaggcctcg gcaaaaacga gctgatgggt cctgaacccc
    23461 gcgcgactaa aaaatcagaa ctcgacatgc aggcggagcg agggagcgag cagaggaccg
    23521 cggtcgcggt tgtagccggg attgttgatg tgctgcaagt cgaaagaagc gaagatcccg
    23581 cgccacacat gcgtggtgta gtaggtctcc acaatatttt cgcggccgta gtttaaagtt
    23641 ccgtcgccga gcaggaagct aatccctccc tgagccagat attcctgatg gtcacgcgaa
    23701 attccattgg aaatgaaggc gaggcctgct cgatcgagct ttcttttcca cggggttcct
    23761 tgcgcggcgg cgccggcctg aacggtttcg tccacttccg tgtaggcata cgactcatgc
    23821 tggccttcgt tccatcccca tcgagaaaat aaacccagcc aatcggtaag aggctgctcg
    23881 atgtttacgc cgaaaccgta ttttgtagtg gtctgcagcg gatgcgcggt aatttcaggt
    23941 tttggagttt tacccgccag gaaatttgcg acggccacgc tataaattcc catgttcgcg
    24001 tggttggcat agccgagcag acgaacaaca gtttcgcgat gcggcacaaa ggcacggtgc
    24061 aactcaagct cgacattttc ggagcgcgcg cgggcgaggt ccgcgtcgag gtttagacca
    24121 ttcgccactt tgggcatcag cgcttccgca aatcgcagcg cccaatggcg gtcatcgtat
    24181 tcgaccatta cagcgtaggt gtagccgcga gtgtccgccg catagtcgta ggcgccgttg
    24241 ttgtccagcg tccaattcat gaactgaaaa ttgctgtccg atccgtaaga attcacgtca
    24301 aaaaaatcca ccaggctgaa ttttccaaag cgaatttcca gccgccgcac cggcaattcc
    24361 ttaaagagag ataatggagt tctctccgat ggcgctttct ctttaccgag cggaataatc
    24421 tggcgccaca tcaggcgagc tacgtacgga gctttgctga gggtcgggtt tcggaccaca
    24481 tccaaatttg tgaaacccgc gagtccgagc gcttccccaa tcccatgccc gccggtttcc
    24541 tgtacatcca gcagaatttc cgagctgtta ttgattcgca ggcccgtaag caaagtaagc
    24601 acgcgcgagg aagcgttctg cgcttcaggc gaaaggctat tcggaccctg atagggagaa
    24661 cgaaatgccg ggtgccactg cgtaataaga tttacctgac cggaaagcca gatgcgctcg
    24721 gactcaaaat gcgggaatag cgtcgtaaca tcatccgacg aggcattttc cgctggtgat
    24781 tgtgcagttt gtatcggctg ctgatctagc ggtgcttcct gagcttggac ggaaaatgtc
    24841 ctgaaaagaa atagtccgag cagacacaag aatgagagca tgaatttagc cgtatgggac
    24901 tgcatcttct tccaattgaa ccgaaatacc cgcttgaagt ttcgcgtccc gcgaactggt
    24961 ttacagattt cgaagtgcta cctgagcttc tattagaaca tgctcgcact ggaatttgag
    25021 atgaacagag gatgaaagct tcagatttta aattggtgta gagcgatgtt ttcgaaacgg
    25081 gttttcaccg acggttgaat cgacttccgg gcttgaatgg tttcattgga tatttctaag
    25141 aggtggagca atttgaggaa atttggcggg ttgggtagag cgcttacaga cccgatctct
    25201 cggggccgtc gtccccgcag cgcgactccc aacccatagc catcttgcga gagtgaagcg
    25261 gttggctcaa tgggcttgaa gattcaaaaa agttgggacc ggggttttag tactaggtgc
    25321 agggaggcca aacgagaagc cctaaatggt tcagtatgaa actgttgcgg gcctatctca
    25381 tgcctaccta taaaaaatta tgaggctctg cttattcaat gatcgcgatt ttgtttgctc
    25441 ctgacaaggg tacccagaga tggcctttgg cgtcacgcgc cattcctgac gctgccgaat
    25501 tcgcagccgg cattggatac gtcctgaaca cttcgacggc ggggtcaaaa cgaaccagcg
    25561 tatttgcatc cttcccgctt tcctcgtacc agatcttgcc tgcggtatcc gcgccaattc
    25621 cgtccggcgc ggaatgaatt cctgaagggc tatcccagaa cttgaatttt ttatcgccga
    25681 gagtaatgcg gccgagtcgc cctccaccga aatccgtaaa gtagatcgcg ccgcctacgg
    25741 caaccaggcg gcgaggtttt acgtcggggt ccgcaggagc atattccgcc atcgttcccg
    25801 ttgcgggatc catgcggccc agcttgccgc cagacaactc gcaaaaccat agtgcatgat
    25861 cgtccgctgc cgcgattcca taaggatgcg cattctgtgt gggcacgcca aactcggtga
    25921 tgattccact gtgagtgtcc agtcgtccga tggcattcga attttccgcg gtgaaccata
    25981 cggcgccgtc cggcccgatt acgatcgagt gcggatcctt taccgttgga ggagtaaact
    26041 ccgaaatggc cccggttttc ggatccatgt gaccaatttt tccggcatta ttttccgcaa
    26101 accagatatc gccttgattg tcgaatacca ggccaatggg accggaatca gcggtcggaa
    26161 ttttaaacag tttccattcg ttcgaattgg gattgaagcg ggcgatgtgc ccggtctgat
    26221 ccagcgtaaa ccaggcgttg ccgttggcat cgatggcgga gtcgcgcggc accgatcctg
    26281 agacaggcgc atcccacaca cgcatcgcgc cctttagttc gaggccgacg ggggcgactg
    26341 gcggtttgaa cgccagccat agcgttacat tcgtatccgc tcctgggcgc accagaatgt
    26401 tgtgttccca ctcggaccag gtgccattcg cggaagcgcg caaatcgtaa aagccagtgc
    26461 gcaacggctc gatatgaaat tggccgtgcg cgtcgctatg caatacgtgc ggggtttcac
    26521 cgtcggcgac ttgccataat atttgcgcat ctgccaccgc gacacctttc gcggtaacga
    26581 cctgtccctg caagccgccc ggcttctgct tgggaacctt aggcaattga gcccacgaac
    26641 tggcgcacag aaagctagcg gcgcacacga ccatcacgat atgtcgattc gtacggcgca
    26701 aagagattgg cctccggaag cttttaccct ttaattttac cttgctgggg ttaatgggtg
    26761 ggggttaccg cgggtagatt gttcaaatca ttacgaacga cttcgatggc ctgggaaggc
    26821 ggaccaattc ggcggtagtg agtgatttcc gtgcgctcat ggatgccgaa agcgaagagg
    26881 aatttgcggc catccacgtc atacgtgtac acagtcggca gccagattcc cggagcgatt
    26941 tcttcctgct ccataacgac gtggccgccg cgataaactt ttccggcgat gcctccggca
    27001 aagggaatgt ccgttgcaat atcgccttcc aagcgcgcaa attgcgcctg cgcttcgtcc
    27061 acccagagaa cagcatgaac atgctgaaag ctggcagcga aacgattgat cggttcgtaa
    27121 tccgggtcag ggttcagcaa aaatttcatg aaggtgtgag gaccgttcga atcggtgcgc
    27181 atctcgcggc ccgcccatgt gattcgaaat gccttcatgg ccgtgtccac aagctcggcg
    27241 cggtcgcgac ggcggcgttc atattttgcg aggtcttctt tgtagtgatc attcggatga
    27301 atggccaggc tgaatatatt ctccgcaatc tggagctcgt tgcggtattc ctgcggcgtg
    27361 acgggaactc cgcgttccgc caattttagt tttatgttgc cggtggtgga cggcaggatg
    27421 cgctctgtaa tgtcgacggt gacgccagcg ttctcgccgg agcgggcaat cttgtgttca
    27481 acgcgctcga attcctggag cgcaagatcg tcacgatgtt gattttgaat ggcccgcatg
    27541 atcaacgcgt gaatctgatc agcgctggga gcgggattgg gggagatgtt tgtaccggtg
    27601 ccactgacca gtgcctgcgc atgagaaaca cttatccacg agacgcctgc gaacaggcat
    27661 attccgagag ctgcgatgtg gaatctctga atcatgagtc gccaactgct gactgaaaga
    27721 cgggtccctc cgctgccatc ttccaataag attctacggc gaagagggcg gttgtactcg
    27781 attgtttcgc aatgagcacg attcgcggcg ggattgcgtg tagaaatgaa agcgggcgcc
    27841 cttatgacca atcagggcgc cgcgccaata cgaatacata tttaccgtgg ataattttgt
    27901 tcgtaacatg gggttgccct attgaaggcc ctccatgagt tttgcgaagg acatgcaagc
    27961 cgcaaggaag tttgtgcgca ggatttattg tgttcgcagc cttttagtgc tgggtgccat
    28021 tcttcttggg gcgcgcggaa gctacgctca agcactgacg cctgcctggg tggaattggg
    28081 tgatgaaggt aaagcgttcg cgcgaattgt cgtaaatact ccacaggatt gccccgcgat
    28141 tcagatcgac ggcgtcaaca agccgatgtc gcttcgaccg aatatgccgg acgggttgcg
    28201 acccgcgtgt gaattcgcaa ttcctgccgg cgcgaaatcg gcttccgtta attcacaatc
    28261 gcttgtgctg ccgaaaaaca acactaccga aatcattacg attggcgata caggctgcag
    28321 aattaagggg cggcaaattc aggcgtgtaa cgatcccgcg ctttggccgt tgcgggaagt
    28381 tgcgtcgagc gccgccgcgg aaaaaccaaa tttgattatt catgtcggcg actatcttta
    28441 tcgcgaaagt cagtgcccgg aagcatcgca ggcgcagtgc gggggcagcc ctgccgggga
    28501 taattgggac gcgtggaacg ccgatttttt tgagcctgcc gcgaaacttt tgagtgctgc
    28561 cccgtgggct tttacgcgtg ggaatcatga agattgcaat cgcgcgtggc gcggctggtt
    28621 ctattatctg gatccgcgcc cgtggagcgg aaagtgcgag gaatattcgg cgccttacat
    28681 cgtcaaactc ggaaactttc agctggcgat gctggattcc gcctggatca acgaaaacat
    28741 agtggacgaa agggaattgc aaattacggt tgcgcaattg agttcgctgc aggcgaagga
    28801 tgcctggctc gtgactcacc atcctttttg gggattctac acggaccagc gaagcgggtt
    28861 gccgaaaccg accactcccg tcttggaaga ggcttgggaa aaagctgagc cgaaaaattt
    28921 taacctgatc ctttctggac acgtgcatct ttttgagtat gtcagcgtgg acaataggcg
    28981 cccgccgcaa attgttgcgg gagatggcgg gacgcaaatg gatgtcccta ttgaaatttc
    29041 ggtgaagggc acgactattc gcggcgcgac tgtcaatgga agcagaatca gggaacaatt
    29101 tgggtacacg atgctgacca aagaagggaa atactggcac ctggaattga agaatcgggg
    29161 agcgaaagtg ctggtgactt gcacggtgcc ggagagcagc gagagctgcc agagcgcggg
    29221 gacggattga tatttttttg cggtggattt ttttgggttt agatgtttgc taagttttta
    29281 cggcaaggct ttcacgcttt acgtcttact tcgctatttg cacaaagacc tcgcctctga
    29341 tccttcgcgt tcactcagga caagagaggc gagctacaaa agaggcgagg ctacaaaaat
    29401 cgttcgtgct tcgcgtttgg caggctctgc gcttacaatg ggcacttcta tgccacgccc
    29461 tagcgagcca ctcacacctg ttagtgaatc ccaagtttta gcgcaacttg ctaggtcacg
    29521 cgggccgcag agccttcgtg agatcgcggc ggctctcgga ttgcgacagt ctggtcgccg
    29581 cgcgctcata aaactcaccc gaaaaatgaa aatgcgtgga gagattatcg aatacccgaa
    29641 ccggcgtatt ggtttgccca agggaaacca aggcgctcaa tttgcggagt cgccgcgaaa
    29701 aggctcgcag cagcggcatg gcgccgagca gcgccaagaa acgcgttcag aatctcgtcc
    29761 ggtgaggcct gaggcgaatc agatgactgg ccgcttggtt gcacatcgcg atggctatgg
    29821 tttcgttgtg ccggatacgc cgcgaaaaga tctggaaggc gatcttttca tcggaagaga
    29881 tggaattggt gacgcgatgc atggcgacca cgtgctggcc agcatcgagc gccgcaagcg
    29941 atttggcgat ggcgccggtc cagcccgcgc ggaagggcgc atcttgcgcg tgattggacg
    30001 cgcgcataca acggttgtcg gtttatttcg atatggattg cgaggcaaca ccgttgcgcc
    30061 gtacgaatcg cggctgctgc aggaaattat tatccccccg ggcgatgaac tgacgccgga
    30121 actgcaaggg aagcttggcg cgcatccgcc aaagcgcggc gtgcgcttgc cggaactcga
    30181 tggcgccgtc gtgaatgtgg aaatgacgcg ctttcctcgc ggaggtgtgg cgccggcggg
    30241 gcgcgtgatt gaaatcctcg gacggccggg cgaattcggc gtggacgtcg agattctgat
    30301 tcgcaaacat catctgcccc atgaattttc gctggaagtg ttggcggaag ccgctgctgg
    30361 agcgcatccc gtgaccgaag cggagcggga agggcggcgc gatttccgcg atttgccgat
    30421 tgtcacgatc gatggggaaa cggcgcgtga tttcgacgat gccgtttacg cgaagcagct
    30481 tgaaaacggc aactggcagt tgcaagtgca tattgccgac gtttcacatt acgtgcggcg
    30541 gggatcggct ctggaccgcg aagcgcggct gcgcggcact tccgtttact tcccgaatcg
    30601 cgccgtgccc atgctgccag aagaattatc gaacggcatc tgctcgctca atccgcggga
    30661 agaccgcctg gtgatgagcg cagtgatgga gctggatgcg aacggcgaaa ttgtcgcatc
    30721 ggaatttgct cgcggcgtga ttcgctccgc ggaacgcatg acgtacaccg cggtgaacgc
    30781 cgtgcttcag aacgatgcgg ccgctaccgc gcagtatgcg catcttgccg gtcatttcag
    30841 cgccatgcgc gatctcgcca tgattttgaa tgcgcggcgc gcgcggctcg gctctatcga
    30901 tttcgatttg ccggagccgg tgatcgaatt taacgatgcc ggacagatga ttggagtcgt
    30961 gcgcagcgag cgcaacatcg cgcaccggct gatcgaagaa tttatgctga cagcgaacca
    31021 ggctgtggcg agctatctcg agtcgcgcgg cgtcggatcg ttgcatcgcg ttcacgaaaa
    31081 accggatccc aagaaaattc tggaatttga ggccatggcg cagacgtttg gctattcgct
    31141 cggcattgaa aatctggccg agcggcgcat ggaagtgaag catggaagtt cgcgccagca
    31201 aaaattcgat cgcggtgcgc ggcacgggcg tcgcggaggc ggacgcgaaa agccgatgac
    31261 ggtttcgctg ccgggagtga cggacatcgc gattcgtcct tcgcattacc aacgtctcgc
    31321 ggaaaaaatt gcgggcaaac ccgaagagcg catcgtctcc tttctgatgc tgcgttcgct
    31381 gaagcaagcg agatatgcgg cggatgcgct gggacatttc gcgctggcct tcgatcagta
    31441 cacgcatttc acttcgccta ttcgccgcta tcctgacttg attgtgcatc gcgtgttgaa
    31501 atgggcgctc gatcatccgg aaacgaaacc cgcgccgaag cctgcgagct acgggagccc
    31561 aagcaaggat gattccacgt tcggcccgta tcgtcgcggc gagctcgaag cgattgccgt
    31621 ggaaacttcc gagaccgagc ggcgcgctga aaccgccgag cgcgaattga tggcatggaa
    31681 gaccgcgcag ttcatggaac agcatttggg cgaggagtac gaagcgctga ttatttccgt
    31741 gcagaaattc ggattttttg tcgagctgac ggagttgttt gtcgagggca tggtgcccat
    31801 cgaccgcatc caggaactga ccggggagca tgttttctac cgcgagcagg atcatgccat
    31861 cgtgagcggc agcggccatg gccaacgcac cgcgtcgcgc ggcgggcatg aggcagggcg
    31921 tgacaaaaat gctaagaaaa aatccgggcg aggcgtgtct cgttccgatc aatccgggca
    31981 tgtctggaag ctcggcgacc gcattactgt gcgcgcggaa cgcattgacc ctattcgccg
    32041 ccgcgtggaa ttttccatcg tgtaagtgat cccctaaagg gtcatgggga tttgtgaacg
    32101 aaggaaagcc ttgaaccggt cactccgcgc ttgctacaat acaactagtt taagctagtt
    32161 gtattggagg cagaatggat tcgattgggg cctttgaggc gaagactcat ctggcggaat
    32221 tactggatcg agtcgcgcgc ggcgagaaaa ttacgattac ccggcacggc gtgcctgcag
    32281 cgttgctggt tcccgtcgag gaaaaagaag ccaggatgac gcaccgggaa atcgtggagg
    32341 gaatgcacgc gctccgtaag aaagtgaagc gaagcaagac gagtgtccgt gacatggtcg
    32401 aagaggggcg ccggttttga agggaatcgt cattgatgct tccgtcgcgc tcgcctggtg
    32461 tttccctgat gaggcaagcg attatgcaga gtctgttttg ctggcactgg aaaagcgagc
    32521 cgcaatggtt cccgcgattt gggcttcgga aattacgaat gccttgctgg ttggcgaacg
    32581 gcggaaccgc ctaggacgcc cggaagtacg gcgcttcgtc gaactgctga agggactgga
    32641 aattgttcag gacgtgcaaa cggtcaccga taccatgaat catgtttttc cgctcgccca
    32701 ggaataccgg ctttccgctt atgatgcggc gtatctggat gtcgcggttc gccgccaaat
    32761 cccgcttgcc acgctcgact ccgatttacg aaaggcggct cttgccgccg gcatggaaac
    32821 attcaacgca taatgttctc cgaacttatt ggtacggaat tcggccagtg ccgatgaagc
    32881 gattgcttgc atcggccctg actgaaatgg atgctaacgc ttacttcgct ttgggttgaa
    32941 acagggctag cgtggcgcct gtgggatcga tcaggatgct gaacgatccc actcccatga
    33001 tttccgttac gtccttcacc acgttggcgc ccagcgatct ggctttcttg gtcgcggcgg
    33061 caacatcgtc gacatgcacg taggccagcc acatggaggg cgcgccgggc atgggatgct
    33121 tcatgatccc tccgccggtt ccttcgccga ctttaatcat cgtgtaagtt tccgcgccca
    33181 tcgggacatc ttccagggtc caattgaaca gcgatccgta gaagcttttt gccttgctta
    33241 cgtccgttgt gttcagctct atgtgtacaa aagggtttgc cactgttttc tcctttgatt
    33301 tgaaatgata tgaatccaac taaaatctgc ttgtcgatga tgccggcgct caaccaaata
    33361 gtttcacgtc gagggatgag attaagataa attccgcttc tcaatcttgt taaattctca
    33421 cttcccggat gcgctctcat tcatatctat gggtttaacg attgggccgg ggaaaaatcg
    33481 acaaacattc aaactatttt tcctgtccat ggattttttg gttctggtga gttgattttt
    33541 gtcgaattct gcccgcccta gtcgttaaac aaacggcagg cgaaaatcgg agaagtcatt
    33601 tccagctgga cttccccgca gggccgctat ataggaccat gagccgccga atgcgtctat
    33661 accgaaaaga ctttaaactt taaggagatt gaaaatggct aactttttgc ttttgctgca
    33721 ccaaacaccg acgagtttcc gcaatctatc gcccgacgaa attcagaaga tcctcggcag
    33781 ctatattgcc tggcgggacg ggttggttaa gcgcaacaaa atgcgcggcg gagagaagtt
    33841 gaccgacgat ggaggccgac atttgcgcac gcaaagcggc aaggtttcag taacggacgg
    33901 tccgtacagt gaatcgcagg aaattcttgg cggttttttc atgatcgaag cggcgaatta
    33961 cgacgaagcc gtggccatcg cgcagacctg cccgcatctg gtggatggca agtggatcga
    34021 gatccgccag gtggacgccg cacactagat cgcaattcaa aacggcgaga agacatgggc
    34081 tgcggcaatg agccgtcagt ttgtcaatgc tgctgtagcc cgcctctctt gtcctgagtg
    34141 aaaacgagga atcggaggtg gggccttttc gtggcgatat cgaggccaga ggtcaccacg
    34201 ccaacgcctg gagattccat ggaaccgcaa tccgaaaaca taaatcagct agtggaccat
    34261 ctattccgcc aggaatttgg ccgcatggtt tcgcgcctga cgcgactcct cggcgcgtcg
    34321 cgtttggcgt tggcggaaga cgccgtccag tacgccatgc tgcaggcgct gcaactgtgg
    34381 ccctatcaag gcgtccctga atatccgcaa gcctggctgg cgaaagttgc ctatcgtcgc
    34441 acgctggatg ttttaaagag cgaaagaaaa ctacggtttt acgatgatga aaatgaagtt
    34501 catttgagaa atcagattga ggaattgcaa tccgatgaaa cgccggaatc cctccgattt
    34561 tcgcaggaag tagccgacga acagctggcg ctgatcttta tgtgctgcca tccggcgctg
    34621 ccgcgttcgg cgagcgtcgc tatcacttta aaaatggtgt gcggatttgg agtcgaggaa
    34681 atcgcgcgcg catttcttac gacgggcact gccatcgcgc agagacttgt gcgcgccaag
    34741 cgaatgattc gcgacgagac ccttcgttgc gaagttcctg cccccggaca actggacgaa
    34801 cgtctcggcg cggtgctcga agttctttac cttcttttta cggaaggata cgcggcgacg
    34861 gcgggcgatc acctggtaaa aaatgatttg tgcgctgagg caatccgctt ggggaaattg
    34921 ttggcggaaa attccacgac gtcgcagccg gagtgccatg cgcttctggc cctgatgttg
    34981 tttcaggcgg cgcgcctgcg agaacggacg cgctgggaga tttgctgctg atcgaggaac
    35041 aagaccgcag tttgtgggac aagtccatga ttcatctggg cctgcttcat ttggcgcaag
    35101 cagcgcgagg taatcatctt tccacatatc acctgcaagc cgaaattgcc gcgatgcatt
    35161 cgaccacgga acattccact cacaccaact ggaaaaatat tttggacctg tacgatttgt
    35221 tgtgggaaaa attgccaact ccggtggttt tgattaatcg tgccgtggcc atcgcacagg
    35281 taaaaggccc ctccgccggc ttgaaagcgc tgaaggcgat tcccgtggac acgcagctcg
    35341 aacgctatcc attccttcat gccgccgagg gcgaatttca ccgccaactt gggaattttt
    35401 cggaagcgga aatttcattt ctcaaggctc aggagtttgc gcgcactgag ccgcagcaac
    35461 ggtttctatt gcgaaagttg gaggcagtgc gcgcaaatga aaagaagcaa taaaaaaacc
    35521 gtccactcca gtatcgaagt ggacggccga gcgggcttaa ctcgaattgt gaacgttagt
    35581 ctttatcgta tttcaggcat tcctggagtt ggcgcagaga ctcatcgatc gtcttgatgg
    35641 cttcgacgcg gtgcccgcca aaatcatgcg cggcatgctc cagatgaccg cgggctcttc
    35701 gaagcgactc gatggcttcg cggatttcgg gatgaggttc aggcaagggc gcgggggcgg
    35761 ctgccggttg cggcgatgtt gctgagactg ccgctgttgc ggtggacttc ggcgatgcct
    35821 gagtcgtgct cacggaaaag caaagcgcag tgcaagaacc gtcaccccaa aaatggtcag
    35881 gatttttatt ttcattattc gctcccatga tcgtgaaagt tttctcggcg gctcccgaat
    35941 tagccggaga cgacagtatt gtagccatgg attcgggatg tgaaattcgc gatcgcgggg
    36001 tcatgtccca tgtataaata gcgctccgaa cggcgggaaa gatatgcatc ctgaaattcc
    36061 aatttgtccg tagtgcaatt tgcataaata cgaagacgga atattccatc gattattcgt
    36121 cggggcttgc cattgaattg cggtagcgga aaagctgtcc gcccgaaaag aagggcatgc
    36181 gctacaaaaa caacggcaaa gcgttacggt tttgtagagg gcgctttgcc acgcggattg
    36241 aggcactgcc gtttaatatc ttttcttcca ggcttgcttt cgattattgt aggtgctcca
    36301 tcgtggaggt gagccgtggc tgttcgcatg tgtccgcatt gcaaaacgga aatctcagcg
    36361 ccgatttccg cggcgcgttc gaatgatatg gaatgtccga agtgcgaagc gcggctggag
    36421 gtggtttccg gatcgcgcac catttccact ctctgcggcc tggcggcggg gacgattgcc
    36481 tggcgcctct caaggg
//
LOCUS       DASZMB010000067         8604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3914437, whole genome shotgun sequence.
ACCESSION   DASZMB010000067 DASZMB010000000
VERSION     DASZMB010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8604
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3914437"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..267)
                     /locus_tag="VG272_03125"
     CDS             complement(19..267)
                     /locus_tag="VG272_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWF12701.1"
                     /translation="MIKKRKGKNIVGRRIAEARQMCKPALTQDALSGKLAQLGIQLDR
                     AAIGKIEINHRHVLDYELKAIAHVLGVDANWLLGIEKS"
     gene            1229..3649
                     /gene="gyrB"
                     /locus_tag="VG272_03130"
     CDS             1229..3649
                     /gene="gyrB"
                     /locus_tag="VG272_03130"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HWF12702.1"
                     /translation="MAEKAAAIRNEPEHRYDAGSIKVLAGLEAVRKRPAMYIGSTGEQ
                     GLHHLVWEVVDNSVDEALAGYCDEINVVVHEDNSVTVTDNGRGIPVDMHATEKRAAAE
                     VVMTVLHAGGKFDSDTYKVSGGLHGVGVSVVNALSDWLELEIWREGEVWEQTYNEGKP
                     KGKLKSTGKTRKTGTKITFHPDPTIFEDHKYSYDILQQRLRELAFLNKGLKITLTDER
                     SDKSVEFRFSGGIAEFVKHLNKGKQVLHDSPVYMEGKKDTVEIEIALQYNDSYTENIY
                     AFANTINTVDGGTHLSGFRSALTRTINNFASATNMLKEQKDEVTITGDDVREGLVAVV
                     SVKLPQPQFEGQTKGKLNSDIKGPVEQLINERLGEYFDKHTTVARRIVGKCIDAARAR
                     EAARKARELTRRKSAMDSGGLPGKLADCQERDPARCELFVVEGESAGGSAKGGRDRKY
                     QAILPLKGKILNVEKARYDKMLGHEEIRAIITALGAGIGKDDFDITKLRYHKIILMTD
                     ADVDGSHIRTLLLTFFFRHMTELIKREHIYIAQPPLYRVKRGKMDRYIRDEDEFNHEL
                     MSRATEDHVVKPKEGPAMEGAGLTKFLLNVQEYVSASAKMVRKLREPELVDILAASNL
                     EKKTDFEDKKALEKLAKAIDKAKLDIDTKIVYDEEHSLYDLLIPGNASHPGDKKINWA
                     FASTPEYKRLRASANLISEFDRPPFTVTRNGDKVVKEKASDVLTYILEDAKKDFTITR
                     FKGLGEMNPDQLWETTMNAETRTLLKVRLDDATAAEEIFSTLMGENVESRRKFIEENA
                     LEVVNLDI"
     gene            3851..4327
                     /locus_tag="VG272_03135"
     CDS             3851..4327
                     /locus_tag="VG272_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWF12703.1"
                     /translation="MGRIARRVHQAGVYFVTTDSWEKHPLFVNTTLANIVIEQIVSCR
                     DRGFYKLHAFVLMSDHLHVLLTPGEATTVEKAMQMIKGGSAHQIGKEKPQKFPIWHSG
                     FHDRWMRDEQEYRGSKRYIEQNPVEAKLAECPENYAYPSASGKYVLDLSQFDQVRG"
     gene            4462..4707
                     /locus_tag="VG272_03140"
     CDS             4462..4707
                     /locus_tag="VG272_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12704.1"
                     /translation="MFYQLSWTTLPGLRGLSCSDFRATPTSEPDQVRGVAMECASDAE
                     RDSLVAELESHFGPQRFLNTAAAFDGVKDYIAQRAQQ"
     gene            4802..7471
                     /gene="gyrA"
                     /locus_tag="VG272_03145"
     CDS             4802..7471
                     /gene="gyrA"
                     /locus_tag="VG272_03145"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HWF12705.1"
                     /translation="MADEIKQSHMIPVEIEAEMKKSYLDYAMSVIVARAIPDVRDGLK
                     PVQRRILVAMNDLGLASNRGYRKCAKICGDTSGNYHPHGEAVIYPTLVRLAQSFNMRY
                     PLIDGQGNYGSVDGDPPAAMRYTEARLAKITEEILADLEKETVDFIPNFDQTREEPVV
                     LPSKTPNLLVNGASGIAVGMATNIPPHNLGEIIDATVLLIEKPDSTLKEVMKFVPGPD
                     FPTGGYISGAEGILSAYKTGRGSLTMRAKAAIESMGKDRENIVITEIPYQVNKSRLIE
                     RIAELVQTKKIEGISDVRDESDREGMRVVIEIKRGEEPKIILNHLYKLTQMQESFGMI
                     LLSIVNGQPRELGLVPMLKLFIDHRVEVVRRRTQFELRKAESREHILVGYHISLDHVD
                     NVIKIIRGSSSRANARENLHDFFENKEVKILTAGNKTETLKGIKLDRKKYGLLDVGFG
                     AVQAGAGIGLSYVQIDAILELQLHRLTRLSVDEILKELAEIRALITELREILASDKKL
                     KAVIVKELREIQKDYADARRTEIVGNVEEIKLEDLIADVEMAITVSHAGYIKRTPVDV
                     YRHQSRGGKGRIGAKTRDEDFVEYLFVASAHSYILLFSSKGRVYWLKVYEIPEAAAAT
                     RGKAISNLVKFQEDEHLTAVVAARDLEVEGTFIFLATRNGTVKKTSLNEFSNPRSTGI
                     IAINLDQDDELIGAHLTDGKQMIFLASHDGQAILFRETEVRSMGRNAGGVNGMDLAKG
                     DYLVGMEPVQPDFEIIKKETKADTQNLDDIENEALKDSLTSLMLTVSEKGFGKRTPLA
                     EYRITSRGGKGVINLKSTDRNGPVVAVLQVAEESDVMIITEYGKVIRVHGNEIREAGR
                     STQGVRLLRLEDDDKIAAAASIQEDEEVTEEKK"
     gene            7497..8351
                     /locus_tag="VG272_03150"
     CDS             7497..8351
                     /locus_tag="VG272_03150"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HWF12706.1"
                     /translation="MGGMDAQLKFLPPIVTSHLFPALEEKLIELLRGLAPEDWEKQTL
                     APKWKVKDVATHLLDTQTRKLAAARHGYKPENSKKLSAEKLISLINTLNAEGVRQYGQ
                     LPPDELISRMEIASRESAEYHRALDPFGVAMFPVSWAGEEESTNWFDTAREFTERWHH
                     QQQIRVAVNKPGIMTREFYFPVLDCFMRALPYTYRNIEAKTGSLAQFNVSGECGGGWY
                     LLRDRDGWKLIASPAGGIISETTIPQEIVWRIFTKGIAADEARPQVRVTGDESIGLHI
                     LKMISIVG"
BASE COUNT         2274 a         2181 c         2385 g         1764 t
ORIGIN      
        1 attgggaagt cgggtctctc atgatttttc aatcccaagc agccaattag cgtcaacgcc
       61 taatacatgt gctatggcct tcagttcgta atcgagaaca tggcgatgat taatctcaat
      121 ttttccaatt gcggctcgat cgagctggat tcccaactgt gcaagttttc cggaaagggc
      181 atcctgcgtc agtgctggct tacacatttg acgtgcttct gcaatacgcc tgccgacaat
      241 gttcttgccc tttcgtttct tgatcacagg gagagagtac cttttgcaaa tggcaataat
      301 gttctaaact agaacaataa agtgaaggga acatcgagca cgaattacga actcttaaag
      361 gaaattagaa gatgaaaagg aaaaaatcac tgtcaaacaa aattagtgca cggccaacac
      421 acaataattt gccgtggccc ggacttaagg ctaaagatag gattagccga gttgtaactc
      481 gtgccgtgaa agacgccaag attacaaatg aacgtgaaga atgggcggag gaacagattg
      541 ctagaatctt ggatatcata ccgccgggcc aagccatgag attaatcatg cgcgaattac
      601 ggcgtcggca gaaatcaatg ctccctttcg aaaagactca tgtttcttga ggatggaatc
      661 taacgaaatg gcctctataa gagtcaaaga aactgctcaa ctgccacatc aatcgtagat
      721 aattggtgcc caagactaag aatgtctcca gatataaacc ggcatccctg aaccattagt
      781 cttaaaatgt aaataattga atataaaaga cttgttgcgt tgacctaagt ccccaatcac
      841 ttgagaaaaa gtctcaaaca ggatagaatc ttggtggtca gatcaacaac tagtttcctc
      901 cagcacggca cgtcggccag gctggcagcg cccctgattt gcatgcccgc gccagagagc
      961 atgcccttcc cggcacatgc gggttagggt aaacgggacg cttgactgcc tggaggaaag
     1021 aagccttcgc cgcgtccgct tccacgcaat ggaatccggg cgggatggag caggaaccct
     1081 tcgagctccg gcaatacgga tttgccgaga gccacagggt aaggtgacgg tcgatacgat
     1141 tgtcgcgggc gttttttttc aaaaaattag ctcgccatca ataatatcag cgcgcctttc
     1201 cccgtaaatt cgcaggagca tgcaaactat ggctgaaaaa gcagcagcaa ttagaaatga
     1261 gccggaacac cgctacgatg ccggatcgat taaagttttg gccggtctcg aggctgtgcg
     1321 caaacgtccc gccatgtaca ttggctcgac gggcgagcaa gggctgcatc acctcgtctg
     1381 ggaagtggtg gacaattccg ttgatgaagc gctcgccgga tattgcgacg aaattaatgt
     1441 tgtggtgcat gaggacaaca gcgtcacagt gacagacaac ggccgcggca tccccgttga
     1501 tatgcacgca acggaaaaaa gggcggcggc ggaagtcgta atgaccgtgt tgcacgccgg
     1561 gggaaaattc gacagcgaca cttacaaagt ttcaggcggg cttcacggtg tcggcgtctc
     1621 cgttgtgaac gcgctgtccg actggctcga gctggaaatt tggcgcgagg gcgaagtgtg
     1681 ggagcagacc tacaacgaag gcaagcccaa gggaaagctg aagagcaccg ggaaaactcg
     1741 aaaaaccggc acaaagatta catttcatcc ggaccccacg atttttgagg atcacaaata
     1801 tagttatgac attctgcaac agcgcttgcg cgaactcgct ttcctgaata aaggcctgaa
     1861 aatcacgctg accgatgagc gctccgataa atccgtcgag ttccggttct ccggcggcat
     1921 cgccgaattc gtcaagcatc tgaacaaggg caagcaggtg ctgcacgatt ctccggtgta
     1981 catggagggc aagaaagaca cggtcgagat cgaaatcgcg ctgcaataca acgacagcta
     2041 tacggaaaac atttatgcgt ttgccaacac gatcaataca gtggacggag gaacgcacct
     2101 ttccggcttc cgctccgcgc tgacgcgcac gatcaacaac ttcgcttccg ccaccaacat
     2161 gctgaaagag cagaaggatg aagtcacgat cacgggcgat gacgttcgcg aagggctcgt
     2221 cgccgtggtt tccgtgaaat tgccgcagcc tcagttcgag ggccagacga aaggaaaact
     2281 gaacagcgac atcaaaggcc ccgtcgagca actgatcaac gaacgccttg gcgaatattt
     2341 tgacaagcac acgacagtgg ctcggcgcat tgtgggcaag tgcattgacg cggcgcgcgc
     2401 gcgcgaagct gcccgcaagg cgcgcgaatt aacgcgacgc aaatccgcca tggattcggg
     2461 cggcctgccg ggaaaacttg ccgactgcca ggaacgcgat ccggcacgct gcgaattgtt
     2521 tgtggttgag ggagagtccg caggcggctc agccaaaggt ggacgcgacc gcaaatatca
     2581 agcgatcctg ccactcaaag gaaaaattct taacgtcgaa aaagcgcgtt acgacaaaat
     2641 gctggggcat gaagaaattc gcgccatcat cacagcgctt ggcgcgggca tcggcaagga
     2701 cgacttcgat atcaccaagc tgcgctatca caaaatcatt ctcatgaccg acgcggacgt
     2761 ggacggcagc cacatccgca cgctgctgct caccttcttc ttccgccaca tgaccgagct
     2821 aatcaagcgc gaacatatct acatcgcgca gcctccgctg tatcgcgtga agcgcggaaa
     2881 gatggatcgc tacatccgtg acgaagacga attcaaccac gagctgatgt cgcgcgcgac
     2941 ggaagaccat gtcgtgaagc cgaaggaagg cccggcaatg gaaggcgccg gactcacaaa
     3001 atttctgttg aacgttcagg aatatgtctc ggcatcggcc aagatggtgc gcaaacttcg
     3061 cgagccggag ctcgtggaca ttctcgccgc aagcaacctg gaaaagaaaa ctgatttcga
     3121 ggacaagaag gccctggaaa agttggctaa ggccatcgac aaggcgaaac tcgacatcga
     3181 cacgaaaatt gtttatgacg aagagcacag cttgtacgac ttgctgatcc cgggtaacgc
     3241 ctcgcatccc ggcgacaaga aaattaactg ggctttcgct tccacgccgg aatataagcg
     3301 cctgcgtgca tccgcgaact tgatcagtga gttcgatcgg cctcccttca ccgtgacgag
     3361 aaatggcgac aaggtagtga aggaaaaagc ttccgacgtc ctcacttaca ttctggaaga
     3421 cgcgaaaaaa gatttcacga tcacacggtt taaaggcctc ggagaaatga atccggacca
     3481 gttgtgggaa accacgatga acgccgagac gcgcacactg cttaaagtgc gcctcgacga
     3541 cgccaccgcg gccgaggaaa ttttctcgac gctgatgggc gaaaacgtgg aaagccgccg
     3601 taaattcatc gaggaaaatg cgctggaggt cgtgaacctg gatatttaaa ggtctttgat
     3661 cccatataca gggatgattt ctgatgcgca gatttcttca gaacgagaaa gtgtttgggt
     3721 tcagttaagc tggcttcatg aagcgaaata aaaaatggaa ttgcgtgtta agaaatgttt
     3781 gtgggtcgcg gcttcagccg cgacataacg aggtccaaga agcggggctt tagcccctga
     3841 gggtgctgat gtgggaagga tcgcgcggcg ggtacatcag gcgggggttt attttgtaac
     3901 gactgattcc tgggagaagc atcctctctt tgttaacacg actttggcca atatcgtcat
     3961 cgaacagatt gtaagttgtc gggaccgcgg attttacaag ctgcatgcct ttgtgctgat
     4021 gtctgaccat ttgcatgttc tcctgactcc gggagaagca acaacagttg aaaaagcaat
     4081 gcagatgatt aagggtggtt cggcacatca gataggcaaa gaaaagcccc aaaagtttcc
     4141 gatttggcat tcgggttttc atgatcgctg gatgagggac gagcaagaat atcggggcag
     4201 caagcggtac atcgagcaga atccggtgga ggcgaagctg gccgagtgtc ccgagaatta
     4261 tgcgtaccca tcggcgagtg ggaagtatgt gctcgacctc agccagtttg atcaggtcag
     4321 gggctaaagc cccttatcta atggctggct aatgtcgcgg ctgaagccgc gacccacaaa
     4381 gatcaaagat agatcgacag atcaaagacg ggtcacagaa ggatcgcgca aagttcaaca
     4441 gtcaacaggt cgacggggga aatgttctac caattaagtt ggacgacgtt gccggggctg
     4501 cgcggattgt cctgctcgga ttttcgagcg acgccgactt cggaaccgga tcaggttcgc
     4561 ggcgtcgcta tggagtgcgc gtcagatgcg gaacgcgact cactcgtggc ggaactcgaa
     4621 tcgcatttcg gcccgcaaag atttttgaat accgcggcgg cgttcgatgg ggtgaaggat
     4681 tacatcgcgc agcgagccca acagtgattg gcatataagt agatttttat actgtagggg
     4741 tacggttcac tgagaacgtt caccgtgaac ggcatgcccc tacacgatga aggacggatc
     4801 gatggcagac gaaattaagc agtcacacat gattccggtc gaaattgagg ccgagatgaa
     4861 gaagtcgtac ctcgattacg cgatgagcgt gatcgtggcg cgcgcgattc ccgatgtgcg
     4921 cgacggcttg aagccggtgc agcggcgcat cttggttgcg atgaacgacc tgggcctcgc
     4981 ctccaatcgc ggctaccgca aatgcgcgaa aatctgcggc gacacttccg gcaactacca
     5041 cccgcacggt gaggccgtaa tttatcccac gctcgtgcgc ctcgctcaat cgttcaacat
     5101 gcgctacccg ctgattgacg ggcagggaaa ctacggctcg gtggacggcg atccccccgc
     5161 ggctatgcgg tacaccgaag cgcgccttgc gaaaatcacc gaagaaattc ttgccgacct
     5221 cgaaaaagaa accgtcgact tcattccaaa cttcgaccag acgcgtgaag aacccgtcgt
     5281 gctgccgtcg aagaccccga acctgctcgt gaatggcgcg agcggtatcg ccgtcggcat
     5341 ggccacaaat attccgccgc ataatttggg cgaaatcatt gacgcgaccg tgctgctgat
     5401 cgagaagccg gattcgacgt tgaaggaggt aatgaagttt gttcccggcc ctgattttcc
     5461 gaccggcgga tatatttccg gcgccgaggg aattctttcc gcgtacaaaa cgggacgcgg
     5521 aagcctaacc atgcgcgcca aagccgcaat cgaaagcatg ggcaaagacc gcgaaaacat
     5581 tgtcatcact gaaattccat accaggtaaa caaatcgcgc ctgatcgagc gcatcgccga
     5641 gctcgtgcaa acgaaaaaaa ttgaaggcat ttccgacgtg cgcgacgaat ccgatcgcga
     5701 aggcatgcgc gtggtgatcg agatcaagcg cggcgaagag cccaagatta ttttgaacca
     5761 cctctacaag ctcacgcaga tgcaggaatc gttcggcatg attctactgt ccatcgtgaa
     5821 tgggcagccg cgcgaactgg gcctcgtgcc catgctcaag ctgttcatcg accaccgcgt
     5881 ggaagtggtg cggcggcgca cgcagttcga gttgcgcaag gccgaatcgc gcgaacacat
     5941 cttggttggc taccatatct cgctcgatca tgttgataac gtcatcaaga ttatccgcgg
     6001 atcttccagc cgtgcgaatg cgcgcgagaa tttgcatgat ttctttgaga ataaagaagt
     6061 caagattctg acggcgggca acaaaaccga aactttgaaa ggcatcaaac tcgatcgcaa
     6121 aaaatacggg cttctcgatg tcggcttcgg cgctgttcag gccggcgctg gaatcggact
     6181 aagctacgta cagattgacg ccatcctcga attgcaattg caccggttga cgcggctttc
     6241 cgtcgacgaa attctgaagg agttggccga gattcgcgcg ctgatcactg agctgcggga
     6301 aattctggcc agcgacaaaa agctgaaagc cgtgatcgtc aaggaattgc gcgaaatcca
     6361 gaaagattat gcggacgcgc ggcgcaccga aattgtaggg aacgttgagg aaatcaaact
     6421 cgaagattta attgccgacg tcgagatggc catcaccgtg agccacgcgg gctacatcaa
     6481 gcgtactccg gtggatgtct accgccatca gtcacgcggc ggcaagggac gaatcggcgc
     6541 aaaaacacgc gacgaggatt tcgtcgagta tctcttcgtc gcatcggctc acagttacat
     6601 tctgctcttt agctcaaagg gacgcgtgta ctggctcaag gtatacgaaa ttcccgaggc
     6661 cgcggcagcc acgcgcggca aggcgatttc caatctggtg aaattccagg aggacgagca
     6721 tttgacggcc gtcgtcgcgg ctcgcgatct ggaagtggaa ggcacgttca tcttcctcgc
     6781 cacgcgcaac ggcacggtga agaaaacttc gctcaacgaa ttctcaaacc cgcgctcgac
     6841 gggcatcatc gccatcaacc tcgatcagga tgacgaactg atcggcgcgc acctcaccga
     6901 cggcaaacag atgatttttc tcgcgagcca cgatgggcag gccatcctgt tccgcgaaac
     6961 cgaagtgcgc tccatgggcc gtaatgcagg tggcgtgaac ggcatggatc tcgccaaggg
     7021 cgattacctt gtgggcatgg aacccgtgca gcctgacttt gaaatcatca agaaggaaac
     7081 gaaggctgac acgcagaacc tggacgatat cgaaaacgaa gcgctcaagg attcactcac
     7141 atcgttgatg ctcaccgttt ctgaaaaggg cttcggcaag cgcacaccgc tggccgagta
     7201 ccgcatcaca tcgcgcggcg gcaaaggcgt aatcaatctg aaatcgacgg acagaaacgg
     7261 ccctgtagtc gcggttttgc aggtggccga agaatccgac gtcatgatca tcacagaata
     7321 cggcaaagtc attcgcgtcc acggaaatga aatccgcgaa gcaggccgct cgacacaggg
     7381 cgtacgcctg ttgcgcctgg aagacgacga taaaatcgcc gccgccgcga gcatccagga
     7441 agacgaggaa gtgaccgaag aaaagaagta gtgacaatca gggaattaga aataaaatgg
     7501 gcggcatgga tgcacaactt aaattcttgc cgcccattgt tacttcgcat ctttttcctg
     7561 cgctggaaga gaaactgatt gagcttttgc gcgggcttgc gccggaagat tgggaaaagc
     7621 aaacacttgc tcccaagtgg aaagtgaagg atgtcgcgac gcatttgctc gatacgcaaa
     7681 cacgcaaact ggctgctgcc agacatggct ataagcccga aaactccaaa aaactctctg
     7741 cggaaaaact gatttcgctg atcaacacac tcaacgcgga aggtgtgcgc cagtatgggc
     7801 aattgcctcc ggacgaatta atttcgagga tggaaatcgc gtcaagagaa agtgcggagt
     7861 accatcgggc gctcgaccca tttggtgtcg cgatgtttcc ggtgagttgg gcaggggagg
     7921 aagaatccac caattggttc gatacggcgc gggaatttac ggaacgctgg caccaccagc
     7981 agcagattcg ggtcgctgtc aataagccgg ggatcatgac gcgagaattt tacttccctg
     8041 tgctcgattg ttttatgcgt gcgcttcctt acacctaccg aaatatagag gccaagacgg
     8101 gatccctcgc gcaatttaat gtaagcggag aatgtggcgg gggttggtat ctgttgcgtg
     8161 atcgcgatgg atggaaactg attgcttcgc ccgctggggg aataatttct gaaacgacga
     8221 ttccgcagga aattgtgtgg cgcattttta caaaagggat tgctgcggac gaagccaggc
     8281 ctcaagttcg agtgacggga gatgaatcga tcggtctgca cattttaaaa atgatttcga
     8341 ttgtgggtta agtggcatgg gcttcagcct gtggaatttt gtccttgcaa ggactaaaac
     8401 ccacaggctg aagcctatgc cacaaaaggc gcaaattaat tcgcttcgag gtaatatccc
     8461 ggccgcgttc gcactttcac tcctggccga tccactgtga ccttgatctg gtgataacca
     8521 ggattctccg cgctagtggg acgatagctc agcatgtatt gcgaattgag ttcgccgccg
     8581 atttcgtcga tcgcggtttc aatt
//
LOCUS       DASZMB010000068        18318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4736632, whole genome shotgun sequence.
ACCESSION   DASZMB010000068 DASZMB010000000
VERSION     DASZMB010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 18318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18318
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4736632"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..1131
                     /locus_tag="VG272_03155"
     CDS             307..1131
                     /locus_tag="VG272_03155"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HWF12707.1"
                     /translation="MRIIKAWWLLGCAFALVSVAYAYQAPFREYPSVEYGSPPVPADY
                     QEKTEFAFARMMYPPGWNDGYRGRFDGDWRKGLSLWTQDYPPADRAFSQAVRRLTRIH
                     TRSVEECVNLDDGDEVYNWPFLYAVQVGEWGPTDKQMLKLRDYLLRGGFFMADDFHGT
                     VEWQVFEERIKKAFPDRPIVEIPNNDPIFHTMFDLDDRYRITGAEHLRVGYKLDGVDA
                     HWRGIYDDHGRVMVAISFNSDIGDSWEWSNEPGYPQKFSDLGMRIGVNYVIYAMTH"
     gene            complement(1344..2381)
                     /locus_tag="VG272_03160"
     CDS             complement(1344..2381)
                     /locus_tag="VG272_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HWF12708.1"
                     /translation="MRFQNKAAISATVSALAISLLSGAFPRALPANPGPSGYRILKTI
                     TTGGTEGWDYITMDSDARLAYIGRENHVDVVNVDTGKVVGKVDGLEGTSGLLPLPEFG
                     RGYAMSGDAGAVVIVDLKTLAKIGTVKAGKDPDSFTYEPVTKRVFVMNSSSANATAIN
                     AKDGTPAGTIALGGQPEAAVSDGKGKVYVNITDKDEIVEVDAATMKVTHRWPLAPGTG
                     PSGLAMDRTNRRLFSACDNQKMVVMNADTGKVIASLRTGAGTDGAGFDPESGNAFASA
                     GGAGTLTIIHEDSPESFKVLEELDTQSGARTMTVDMKTHNVLLVTARHGHGSTHNMVL
                     PNTFVVLVVGK"
     gene            complement(2413..3462)
                     /locus_tag="VG272_03165"
     CDS             complement(2413..3462)
                     /locus_tag="VG272_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HWF12709.1"
                     /translation="MQKVIGIAFLLAVSITLTACKGNETSTAPASAPPAAEAVSPMIL
                     TATVPLEGVKGRFDHFASGKGKVFVSALGNNTVEVIDLFQGALAHTITGVPNPQGVTF
                     SPEANKLFVASEKGKVYIYDGDSYKLLATLNFEGGADNMRYDAATKRVYVGCGDSEKN
                     AAIAAIDATTNKRLDEVYKLGGEPESFQLEKNGPNIYVNVPDLKQIVVINRTTRELTR
                     WPVTAGQNFPMSLDEADHRVIVGTREPATLTVLDTNTGKTVASVPTVQDTDDLYYSAE
                     HKRVYVPGRAGEIWVYQQTDPDHYNVITKIPTVVGAGTAGYFGRQGKGFDRFYLAVSA
                     SANSSAEVRIYTLSD"
     gene            complement(3782..4360)
                     /locus_tag="VG272_03170"
     CDS             complement(3782..4360)
                     /locus_tag="VG272_03170"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HWF12710.1"
                     /translation="MPSKEENENWIHPRVALVTGIVVAAAVLRIVPHPLNFAPIGALA
                     LFSGAYFSSKRAGVAVPLLSLLAGDFFVGFHRLVPYVYASFVLSVVIGFWLRRKKSVS
                     RVGAATLAGAIQFFLVTNFAVWVTSTGSYPKNLGGLITCYIAGVPFFWNTLAGDAFYI
                     ALLFGAMALAEKRFPSLREPLCAEEELKLAHR"
     gene            complement(4374..5054)
                     /locus_tag="VG272_03175"
     CDS             complement(4374..5054)
                     /locus_tag="VG272_03175"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HWF12711.1"
                     /translation="MSEAQTPEELLFCWSGGKDSAMALRALRAAPNCRVTALLTTITE
                     DYDRISMHGVRRTLLERQAESIGLPLHLVMIPPQCVNVTYEERMREALDHHVSLGVRR
                     VGFGDIFLEDLRAYREKNLSQMGMRGVFPLWKRDTRDLAREFVRLGFRAIAVCVDSRV
                     LDSSFAGRELDAFFFADLPPHVDPCGENGEFHTFVFDGPIFKSPIAIRTGEKVVRDGF
                     CFCDLLPE"
     gene            complement(5047..7221)
                     /locus_tag="VG272_03180"
     CDS             complement(5047..7221)
                     /locus_tag="VG272_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWF12712.1"
                     /translation="MRFSVRALFFLWSAAFITIIFTSSGVLAQSQTAKLSGTVTDPRG
                     AAIVGAHVTAEPISTGNTAEHGVSTNDGQFLLTLAPGKYRLTIARDSFATAQQEIDLA
                     AGENREIDVRLTLESLSSKVVVTAQALPLDADSSPAPVTILTREQIDQRVANSLPNLL
                     ATQPGLSLGRTGAEGGSASLFLDGGNSNYTKVLVDGVPANTPGGLIDYSNFTLENIDK
                     IEIVHGAESALYGSDAMDGVIQIFTHRGTTRIPEFTAFADGGNFGTGHGGAELSGLLG
                     RFDYSAGVSDMETSGQGPNDAFRNRTLSGNFGWRLSDTARIGLSLRDNDSEAGTPGQT
                     LLQPANLTDTISLHNFGAGLRADFATGSHWHHQLSGTETYFREFNFDPFFQTFYQYNR
                     AAFTGESTYLMRGFAFTAGYENEVENGFLSFIAMHARRNNQSGFLDARWQPFARLTLN
                     AGARAEDNSAYGTRVVPRAGASYVLRTAQGAFGDTRLHATYGEGIVEPRFDQSFGDDP
                     CFPGNPALSPEQSRTIHAGVDQKLASDRVRFTADYFDSRFHNIISFINSPSSIACPFG
                     MGTYFNTDLARARGANLSGEARITRWLSGSASYTYDSTHTLSAPTDIANIDPNYLIGS
                     RLLRRPVNSGNVMLNANYWRMNWNVSGYFSGQRFDYNYPGQIIDPGYALINLAATYNV
                     ARGFSIYGRIANLANKQYQEAYGFPALGREFRIGVKYTVRHE"
     regulatory      complement(7285..7454)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(7597..8475)
                     /locus_tag="VG272_03185"
     CDS             complement(7597..8475)
                     /locus_tag="VG272_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF12713.1"
                     /translation="MNTPNTFAQTSHGTDERPNRAQTVRLNVEQASYAYSASAQSVPT
                     FTLGMTTFQTREREIVAILGPNASGKSTLLRLIAGTIAPLSGRVELDGEETSRLDVRT
                     RAQRIAMVHQESPVVYPALAGDFVLQGRYPYGRALRFANREDIEIARNALSQVGGDHL
                     AKRTISELSGGEKQRVILARALAQQPRLILLDEPTLHLDIGAQVELLERLRQLAQENR
                     YTVVVVTHELGLAAEFADQVVLLQHGRCLRVGSPAAVYQKELLEQVFDAPLDVELGPA
                     GRPRVNLRARRGEDIS"
     gene            complement(8472..9437)
                     /locus_tag="VG272_03190"
     CDS             complement(8472..9437)
                     /locus_tag="VG272_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HWF12714.1"
                     /translation="MLLGSVVLALKLGAVPISVTDLVLDLGRIASGRANELPTEYRLI
                     VFDLRLPRILLGILVGAALSVAGASFQALLRNPLADPYVLGVSSGAALGSILALIIAP
                     GLALITPIGAFIGAIATIAGVYFLGRREGQLDSTTLLLGGIISASFFSAIIMFLMTTL
                     AGRDLRGMVFWLMGDLSTPISAGMQWVFTVGLIAGTGAIYSTSADLNLMLTGEREATH
                     LGVDVMRVKLVVYISASLLTALAVSSSGAIGYVGLLIPHIVRLIFGSDYRLLIPASAL
                     CGAIAIVLADTLARTIAPPTELPVGAMTAMAGAPVFIYLLRRGLR"
     gene            complement(9479..10549)
                     /locus_tag="VG272_03195"
     CDS             complement(9479..10549)
                     /locus_tag="VG272_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HWF12715.1"
                     /translation="MKNTIRAAETLSVKSWKRGRAIVRAAILVLLVIPCGSASAGQPS
                     PGQTDTSRAATPAARELMDEVGRRVRAPQEVDRIVSLAPNLTEIVFALGEGNHLAGDT
                     DFCDYPAEALQKPHIGGPVNPNLEEIVSLMPDLILASKSINRRESVNALDHIGLPVYV
                     TDPHSVDGMITSVEHIGSAIGVEKSANMLTGNLRARLADLDRRISGGAQRRVLFVVWT
                     DPLISVGSDTFIADALRRAGGRSVVDTRVEWPHVSMEEMVKLQPEFIVFASAHTGDAR
                     HDIETLRTRPGWKSLTAIQQGNIIVISDAINRPAPRMVDAIEQLARALHPEAFASRAM
                     PSRDLDIATMNSDIEEACICTH"
     regulatory      complement(10638..10780)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            11073..11507
                     /locus_tag="VG272_03200"
     CDS             11073..11507
                     /locus_tag="VG272_03200"
                     /inference="COORDINATES: protein motif:HMM:NF015608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HWF12716.1"
                     /translation="MVSALLVGHIFGLVFWVSGLLVTSMALTRHTQETSADAKQALAR
                     LERIYLRGLADPGALLTILAGLGLISTNASYYMHAPWLHIKLTFVLLLIGLHGLVAVR
                     TKKFSTGKISMQRSEARMLVAAVLFLFASILVATLVGQVYLT"
     gene            complement(11559..12200)
                     /locus_tag="VG272_03205"
     CDS             complement(11559..12200)
                     /locus_tag="VG272_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12717.1"
                     /translation="MDDPLNTPDSIPSEEPFDTGVPPEATPFAEPLLDLTPAFEPQLK
                     SPEDRHADVIAMYADAAERKRGFRNNLLLLLGLTLAAFILSSWILVRVDSPFGIFSSG
                     PQEVVRAQLRALDHGELRPAYDMFSPRYRGQVSFDTWHELFITHWRMFHADVVRSEIP
                     AQSTSGVTLEIYLHSADDKDYRARFTLVRADGRWWIDDVHWEQEPDERELSRT"
     gene            complement(12240..13064)
                     /locus_tag="VG272_03210"
     CDS             complement(12240..13064)
                     /locus_tag="VG272_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWF12718.1"
                     /translation="MTDLVQTEASFAKPARRTPADFSFIRFQVEGAVARLTLQRPEHN
                     LLNEQMLRELADGVGLIAENGAVKMIILDAAGKLFCAGVDIGEYTAERAFSMLDAFHS
                     ACIAMVEAPQPVLVIVDGAAIGGGAELVAYGDLVVATPRARFALPEITIGMFPPLAST
                     MFPHIVGPKRALELVLTGEAISAERARDLGLVNRLVPEVQLQTAVNEMIGKITAQSGA
                     VLGMAKRAVLGGMGMSLRDSLQNSMKIFLNELYRLEDSQEGLRALLEKRKPQWKNR"
     gene            13232..14503
                     /gene="serS"
                     /locus_tag="VG272_03215"
     CDS             13232..14503
                     /gene="serS"
                     /locus_tag="VG272_03215"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HWF12719.1"
                     /translation="MHELGYFREHLEEFEQMAANRGVKIDFAHFRTIDQERRERITFA
                     ERLKAQRNKASEEIPRKKKAGEDVSALLAEMKQVSEEIKQADERLAELDAQLAFFMLN
                     VPNLLHASVPVGRDAAANVEARRWGAPPKFDFKPRPHWEIAESAGILDFQRAAKMSGA
                     RFAIYRGLGAKLERALAAFFLETHAANGYTEILPPFLVNTAALIGVGQLPKFKADMFH
                     LEGTDLWLTPTSEVELTSFYRDETLDADQLPVKLCAWTACFRSEAGAAGKDTRGLKRQ
                     HQFQKVEMFKFTRPEQSYDELESLVANAELILQKLGLHYRVVGLCTGDMGFASSKTYD
                     IEVWLPSANEFMEISSCSNTEAFQARRSGIRFKPKGGKSEFVHTLNGSGLAVGRTWLA
                     IMENYQQADGSVVIPEVLRPYLGTDRIPAKS"
     gene            15222..16529
                     /locus_tag="VG272_03220"
     CDS             15222..16529
                     /locus_tag="VG272_03220"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HWF12720.1"
                     /translation="MATVVKTVPQPQPILPRMAPREELNPFRIAQIQFDMAAEYLKLD
                     PGLRQILRTPKRVMEVAVPVKMDNGQTKVFTGFRVQHNVSRGPAKGGLRYHPNVTLDE
                     VKALAAWMTWKTATVNLPYGGAKGGVICDPKHMSKGELERMTRRYAAEIQPIIGPEID
                     IPAPDVYTDSQTMAWIMDTYSMAAGHASPGVVTGKPVSIGGSEGRSDATGRGVLFVVE
                     EACKVKKINLRGASVAIQGFGNVGSAVARLFAEKKAKIVAISDSRGGVHNPRGIDPLR
                     AMRYKERAGTVVGMPGASRISNDELLALKCDILIPAALENAITLHNADQIKAKIVAEA
                     GNGPTTPHADEILSRKGIFVIPDILANAGGVTVSYFEWAQDLQGFFWQVQEVNSKLEF
                     VMRRAFNDVHETMRKFHVYPRAAAYILAVGRVADATLVRGLFP"
     gene            16714..17595
                     /gene="thiD"
                     /locus_tag="VG272_03225"
     CDS             16714..17595
                     /gene="thiD"
                     /locus_tag="VG272_03225"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HWF12721.1"
                     /translation="MSNHANVAPPIVLTIAGFDPSAGAGIAADIKTFAAHNCYGVAAV
                     TALTVQSTQGVKSVHDTPAAELRAQLDMLLEDIKFSAIKIGMLGHRGNAVVVAEFLDR
                     AGVANVVLDPVMKSTSGHADLIDAGGIKCISEELLKRATVITPNIAEAEILSGISIKD
                     AASMEGAARKIVERGARAVVVKGGHMDKAVDVLFDGTEVLRFGGDHLKNENTHGSGCT
                     FASAIAAQLASGRPLRESVLLAKAYVTKAIEKGFSIGKGPGPLDHFYRTRTEPPTRGV
                     HEVPQHGMHPAAEPGAH"
     gene            complement(17700..>18318)
                     /locus_tag="VG272_03230"
     CDS             complement(17700..>18318)
                     /locus_tag="VG272_03230"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF12722.1"
                     /translation="LSARSSLELVRSAKAKGLHVTCEVTPHHFTLTDEDVQYDTRYKM
                     NPPLASREDRDALIAGLADGTVDAIATDHAPHEAALKEVEFDRAPFGVTGFETAIGLG
                     LELVHAGKLSLMRLVELFTIGPARVLGKQRRIAENEPGDLTIFSTEQKWTFRAKESVS
                     KSKNSPFDGREFRGGPVATIVAGRMVYIKDSHRDARINTDKSESK"
BASE COUNT         4167 a         5151 c         4946 g         4054 t
ORIGIN      
        1 tgctctgggc aagtgtccca ttcgccgcag ccgtttcctt cagccgcttc tcaacccaag
       61 cgtctcgcac tcccatcgct ttccccctga agagtctccg ctcgcggagc gtctctcttg
      121 tgtaagcccg aattatcgca ctgccgtgcg tgcgcggcaa cctgagtgaa acatgtcgcc
      181 gggttgcgtg gcataggctt cagcctgtgg gttttagtcc ttgctaggac caacttccac
      241 aggctgaagc ctatgccact taaagccaac cgcgcaccgt gttatcctga tcgaagggac
      301 cgggcgatgc gaatcataaa agcttggtgg ctgttgggat gcgcgttcgc tctggtgagt
      361 gtggcgtacg cttatcaagc tcctttccgc gaatatcctt ccgtcgaata tggcagtccg
      421 ccggtccctg ccgactatca ggaaaaaact gaatttgcat ttgcgcgaat gatgtatccg
      481 ccgggatgga atgacggcta tcgcggccgc ttcgatggcg actggcgcaa aggactttcc
      541 ttgtggacgc aggactatcc accagctgac cgtgcatttt cgcaggcggt tcgccgactc
      601 acgcgcattc acacgcggtc ggtggaagag tgcgtaaatc ttgacgatgg cgatgaagtt
      661 tacaactggc cattcctgta tgcggtgcaa gtgggtgaat ggggacccac cgataagcag
      721 atgttaaagc tgcgggatta tctgctgcgc ggcggatttt ttatggctga cgattttcat
      781 ggaactgtcg aatggcaggt gtttgaagag cgcattaaaa aagcgtttcc cgatcgccct
      841 atcgtggaaa ttccgaacaa cgatccgatt tttcacacca tgtttgatct ggatgaccgt
      901 taccggatta ccggtgcaga acaccttcgc gtgggctaca aacttgacgg cgtcgatgcg
      961 cactggcgtg ggatttatga cgatcacggc cgggtgatgg tcgcgatttc attcaattcc
     1021 gacatcggcg attcatggga gtggtcgaac gagccgggct atccgcagaa attttccgac
     1081 ctgggaatgc gcatcggagt gaattatgtg atctacgcga tgacgcacta aggttaagta
     1141 gcgcgggctt tagagtctgt gtgaggactg caaaattgaa gcttcagcgg ctaaagccgc
     1201 actcattttg cagaacttat gtcgtggcta aagccacgac ccacaaagat tctgcagttc
     1261 tcacacagac tcttcagcca gtccttgcaa ggcttacatt ccgcagggga aaatccgcgc
     1321 cacgaatagc tattgtcctt aatttatttt cccaccacca gcacgacaaa agtatttggc
     1381 aacaccatgt tgtgcgtcga accatgcccg tgcctcgccg taacaagcaa cacattatgt
     1441 gtcttcatat ctacggtcat cgtccgcgca cctgattggg tatcgagttc ctcgagcacc
     1501 ttgaagcttt caggggaatc ttcatgaatg atcgtgaggg ttcccgctcc gccggccgat
     1561 gcaaatgcat ttcctgattc cggatcgaag cccgcgccat ccgtgcctgc ccctgtgcgt
     1621 aacgacgcaa tcaccttgcc cgtatccgcg ttcataacca ccattttctg gttatcgcag
     1681 gccgaaaaca gccgccgatt tgttcggtcc atcgcgagcc ctgagggccc ggtgcccggc
     1741 gcgagcggcc agcggtgcgt taccttcatt gttgctgcgt caacctcaac aatttcatcc
     1801 ttgtccgtga tgttgacgta aactttccct ttgccatctg agacagcggc ctcgggctgt
     1861 cctcccagcg caatcgtgcc cgcgggagtg ccatccttcg cattaatcgc agtggcgttg
     1921 gcgctgctgc tgttcatgac gaaaacacgt ttcgtaaccg gctcgtatgt aaatgaatcg
     1981 ggatctttcc cagcctttac cgttccaatc ttcgccagcg ttttcagatc aacgatgaca
     2041 actgcacctg cgtcgccgct catcgcatag cctcgtccaa attcaggcaa aggcagcagg
     2101 ccggaagttc cttcgaggcc gtccactttg cctacgactt tgccggtatc gacgtttacc
     2161 acgtccacat gattttcacg gccgatgtac gcaagcctcg cgtcactgtc catcgtgatg
     2221 taatcccagc cctcggtccc gccagtcgta attgttttca ggatgcgata gccagacggg
     2281 ccgggatttg caggcaaggc acgcgggaat gctcccgata aaagagaaat cgcgagggct
     2341 gaaactgttg cagaaatggc tgctttattt tggaatcgca ttacgattct ccctttctga
     2401 taaacaacgg aatcagtcac tcagcgtgta gattcgaacc tcggcactcg aattggcgct
     2461 tgccgaaacc gccagataga aacgatcgaa ccctttcccc tgccgtccaa agtatccagc
     2521 cgtcccggcg ccaacaacgg tgggaatctt ggtgatcacg ttgtagtgat cgggatcggt
     2581 ttgctgataa acccaaattt cgccggcacg ccctggcacg tagacgcgtt tgtgctcggc
     2641 gctgtaatac agatcgtccg tgtcctgcac ggtgggaacg gaggcgaccg tttttccggt
     2701 attcgtatca agaaccgtaa gtgttgccgg ttcgcgcgtt ccgacaatta cacggtgatc
     2761 ggcttcatcc agtgacatcg ggaaattctg gccggcagtg actggccatc gcgtgagttc
     2821 cctcgttgtg cggttgatca ccacgatctg ttttagatcg ggcacattta cgtaaatgtt
     2881 cgggccgttt ttttccagtt ggaacgattc cggctcgccg ccgagtttgt agacttcatc
     2941 gagtcgtttg ttcgtggttg cgtcgatcgc agcaattgcg gcgttctttt cgctgtcccc
     3001 gcagcccacg tagacgcgtt ttgtcgctgc gtcataacgc atgttatccg cgccaccttc
     3061 aaaattcagt gtcgcgagaa gtttgtaaga atcaccgtcg tagatgtaga ccttcccttt
     3121 ctcgctggcc acaaacagtt tgtttgcctc cggagaaaag gtgacaccct gtgggttcgg
     3181 cacgccagta atcgtgtggg ccaaggctcc ctggaaaaga tcgatcacct cgaccgtgtt
     3241 gtttcctaga gcggagacga atactttgcc tttgccggat gcgaaatgat cgaaacgacc
     3301 cttgacgccc tccagcggaa cagtagccgt caaaatcatc ggagatacag cctcagccgc
     3361 aggcggtgca cttgcaggag ccgtggaggt ttcgtttcct ttgcacgccg taagtgtgat
     3421 tgaaactgcc agcaaaaatg caatgccaat aactttttgc atgacttctc ccaagggaat
     3481 ctaattttct tgcgctccgt aacgattgaa caccgatctt atttgctcgg aagcaaagtt
     3541 cgacgctcgc ttcataccat gaacaaagta ggtgttgatg aacattggaa gatgccagcc
     3601 gtcggcgcga cggcgaattg ctatccgata gatttaatcc ggaaaatgac cggggaacct
     3661 gactggctat gtaccacctc acggaaatcc gtgcaaggaa tatctgattc ctactcgttt
     3721 gcgcgaatgc cgaaatcgca aacgtcgcgg ataaatttaa ataggtcgct ccttgctaaa
     3781 attaacggtg agccaatttt agttcttctt cagcgcaaag cggttcccga agagaaggga
     3841 atcgcttttc tgctaatgcc atggctccga acagcagcgc gatgtagaaa gcatctcccg
     3901 ccagcgtgtt ccagaaaaac ggcacacccg cgatatagca tgtgattaat ccacccaaat
     3961 ttttcggata acttccggtg gatgtcaccc acactgcaaa gttagtgaca agaaaaaatt
     4021 gaatcgcgcc ggccaatgtg gcggcgccaa cgcgcgatac ggatttcttg cgccgcaacc
     4081 agaaaccaat gacgacgctc agaacgaagc ttgcgtatac atagggaacg agcctgtgaa
     4141 agcccacgaa gaagtctccc gccaggagcg atagcaacgg aacggcgaca ccggcgcgtt
     4201 tgcttgaaaa ataggctccg ctaaacaatg ccagagctcc aatgggcgcg aaattcaatg
     4261 ggtgcgggac aatgcgcagc actgccgcgg caaccacaat gcccgtgacc agcgctacgc
     4321 ggggatggat ccaattttcg ttttcttcct ttgatggcat atttttcctc ctctcactcc
     4381 ggcaataaat cgcaaaagca aaatccatct cgcacaactt tttcgccggt ccgaattgca
     4441 atgggcgact taaaaattgg accatcaaaa acaaaggtgt ggaattcgcc gttctcgccg
     4501 cacgggtcca cgtggggcgg taaatcggca aagaagaatg catcgagttc gcgccccgca
     4561 aaagaggaat cgagaacgcg ggagtcgacg caaacggcaa ttgcccggaa accaagccgc
     4621 acaaactctc gcgccaaatc gcgcgtgtcg cgtttccaga gcggaaacac gccacgcatg
     4681 cccatctgac taagattttt ctcgcggtaa gcgcgtaggt cttcaagaaa gatatcgccg
     4741 aaccccacgc gacgcacgcc aagcgagaca tgatgatcga gtgcttccct catgcgctcc
     4801 tcatatgtca cgttgacgca ttgtggagga atcatcacaa ggtgaagcgg caggccgatt
     4861 gattcggcct gccgttcaag gagtgtgcgg cgcacgccat gcatactgat acggtcgtag
     4921 tcctcagtaa tggttgtgag cagagcggta acgcggcagt tcggcgctgc acgcaaagcg
     4981 cgtaaggcca tcgcgctgtc ctttccgccg ctccagcaga agagcagctc ttccggcgtt
     5041 tgcgcctcac tcatgacgca ccgtgtattt cacgccgatg cgaaactcgc gaccgagcgc
     5101 aggaaatccg taggcctcct gatactgctt gttggcaaga tttgcaatgc gtccgtaaat
     5161 gctgaagccg cgcgcgacat tgtaagtggc tgcgagatta atgagcgcgt agccgggatc
     5221 gataatttgt ccgggatagt tgtaatcaaa acgctgtccg gagaaatatc ccgatacgtt
     5281 ccaattcatc cgccagtagt tggcgttgag catgacgttt ccggaattca ccggcctccg
     5341 cagcaggcgg ctgccgatga ggtaattggg atcaatgtta gcgatgtccg tcggcgctga
     5401 cagtgtgtgc gtcgaatcat atgtgtaact cgcgctgccg ctcagccagc gggtaatgcg
     5461 tgcctcaccc gacaaattcg cgccgcgcgc gcgcgccaga tcggtgttga aataagtgcc
     5521 cattccaaaa gggcaggcga tgctggacgg actattaatg aaactgatga tgttatgaaa
     5581 acgactgtca aaataatcgg cggtgaaacg gacgcggtcg gatgccagtt tttggtcaac
     5641 acccgcgtga atcgtgcggc tctgttcggg cgaaagagcg gggttgcccg ggaaacaggg
     5701 atcgtctccg aaggactgat cgaatcgcgg ctccacaatc ccttccccat acgtggcgtg
     5761 caaccgagta tcgccgaacg cgccctgggc agtccgcaac acgtaggacg cacccgcgcg
     5821 aggcactacg cgggtcccgt aggcggagtt atcttccgcg cgcgctccgg cattgagagt
     5881 caggcgcgcg aatggctgcc agcgagcatc gagaaaacct gattgattgt ttcggcgcgc
     5941 gtgcatagcg atgaaactaa gaaatccgtt ttcaacttca ttttcgtatc cggcggtaaa
     6001 tgcaaatcca cgcataaggt acgtggactc gccagtgaag gcggcgcgat tgtattgata
     6061 gaacgtttga aaaaatggat cgaaattgaa ttcgcgaaaa tacgtttccg tgccgctcaa
     6121 ttggtgatgc caatgcgaac cggtggcaaa gtccgcgcgc aagcctgcgc cgaaattatg
     6181 caaagagatt gtgtctgtga ggttagccgg ctgcagaagt gtctgcccgg gcgtgccagc
     6241 ttcgctgtca ttatcccgca gagaaagtcc gatgcgcgcg gtgtcggaaa ggcgccagcc
     6301 gaaatttccg gagagcgttc gattgcgaaa tgcgtcattc gggccttgcc cgcttgtttc
     6361 catgtcggaa actccggcgg aataatcgaa tcgcccaagc aacccggaaa gttcagcccc
     6421 tccgtgaccc gtgccaaaat tgccgccatc cgcaaatgcc gtaaattccg gtatgcgcgt
     6481 tgttccgcga tgcgtgaaaa tctgaatcac gccatccatc gcgtccgaac cataaagtgc
     6541 gctttccgcg ccgtgcacga tttcaatttt gtcgatattt tccagcgtga agttggaata
     6601 gtcgatcagg ccgcccggcg tattggcagg cacgccatcg accagcactt tcgtgtaatt
     6661 ggaattgccg ccatccagga acagggaagc cgatccgccc tccgctcctg tgcgcccaag
     6721 gcttaagccc ggttgcgtcg ccaacaaatt cggaagcgaa ttagctacgc gctgatcgat
     6781 ctgttcacga gtgagaatcg tgacgggcgc gggagaagaa tcggcatcca gcggcaaggc
     6841 ctgcgcggta accactactt tcgatgacaa tgactcaagc gtgagtcgaa catcgatctc
     6901 gcgattttct cccgcagcta aatcaatttc ctgctgcgcc gttgcaaatg aatcgcgcgc
     6961 aattgtcagg cgatacttgc ccggtgcaag cgttaacaaa aattgcccat cgttcgtgga
     7021 gacgccatgt tctgccgtat tccccgtaga aattggttcc gcggtaacgt gcgctcccac
     7081 aattgcggcg ccacgtggat cggtaaccgt gccggaaagt ttcgcggttt gcgattgagc
     7141 caacactcca gatgaagtaa aaataattgt gataaaagct gcggaccaaa gaaaaaataa
     7201 agcacgaacg gagaaacgca tggaggcctc ctctcccgtc tcgcgcggga gataggttgt
     7261 ccgcatgtcc gggatcgttt ccggcttagc ggttgcggac tccgtcggtc tcctggctcg
     7321 gcggattggc accgcgcgcc cttcccaccc cgaacaaaag ggcagtggca cgcgcggaat
     7381 ttctcccgcc ttacagtcgc gcggcggcgg cggtttttca ccgtcttccc gtcacggagt
     7441 ctacggttat tgtggcgaaa cgcagcggca ctggcagggc caggaaaccg cattgcttcg
     7501 ggtgaaatgt tgcggaggct tcggcctacg gggtttagtc tttgcaggaa ccaaaaccca
     7561 aaggccgaag cctacgcaac agatttactg catccgttac gaaatatcct cgccgcgtcg
     7621 ggcgcgcaaa ttcacacgcg gccttcccgc gggaccgagt tcaacatcga gcggcgcatc
     7681 gaacacctgc tcgagcaatt ctttttgata tacagccgcc ggagagccca cgcgcaggca
     7741 gcgtccatgc tgcagcagca ccacctggtc ggcaaattcc gctgcaagtc caagctcgtg
     7801 cgtgacaaca accacggtgt aacgattttc ctgcgcaagc tgcctcaggc gctcgagcaa
     7861 ttccacctgg gcgccgatat ccaaatgtag agttggctca tccagcagaa tcagccgggg
     7921 ctgttgcgcc aacgcgcgcg ctaaaatcac gcgctgtttc tcaccgccgg atagttcgct
     7981 gattgtgcgc ttcgccaaat gatccccgcc cacttgtgaa agtgcgtttc gcgcgatttc
     8041 aatgtcttcg cgattcgcga atcgcagcgc gcgcccgtaa ggatagcggc cctgcagcac
     8101 aaaatctcca gccagtgcgg gataaacgac cggactttcc tgatgtacca tcgcgattcg
     8161 ttgcgcccgc gttctcacgt cgagcctgct ggtttcttcg ccgtccagct cgacgcgacc
     8221 ggaaagcggc gcaatcgttc ccgcaatcag ccgtagcagc gtcgacttgc cgctcgcatt
     8281 cggtcccagg atcgcgacaa tttcccgctc gcgcgtttga aaggtggtca tgcccaaagt
     8341 aaatgtcgga acggattgcg ctgacgccga atatgcgtag ctcgcttgct ccacattgag
     8401 ccgcaccgtt tgtgcgcgat taggcctttc gtcggttccg tgcgaagttt gtgcgaaagt
     8461 gttcggcgtg ttcatcgcag ccctcgccgc agcaaatata taaatacggg cgctccggcc
     8521 atggcggtca tcgcgcctac aggtaattcc gtgggaggcg caatcgtgcg cgccagcgtg
     8581 tcggccagca caatggcaat cgcgccgcac agcgccgaag ccggaatcag caatcggtaa
     8641 tccgagccaa aaattaatct cacaatgtgc ggaatcagca ggcccacgta gccaatcgca
     8701 ccgctcgagg aaacggccag tgccgtgagt aaggaagcag aaatatagac gacgagtttc
     8761 acgcgcatca catccacgcc taagtgcgtg gcttcccgtt caccagtcag cattaaattc
     8821 aaatccgcgg acgtgctgta aatcgcgccg gttccggcaa tgagtcccac ggtgaaaacc
     8881 cattgcatgc cggcggaaat cggagtcgac aaatctccca tcagccagaa gaccatgccg
     8941 cgcaagtcgc gccccgcaag cgtcgtcatc aggaacatga tgattgcgga aaagaatgat
     9001 gcgctgataa tgccgcctag caaaagagtt gtgctgtcca gctgaccttc gcggcggccg
     9061 agaaaataga cccccgcaat cgttgcaatg gctccgatga acgcaccgat cggcgtgatc
     9121 aatgccaatc ctggcgcaat gatcagtgcg agaatcgagc caagagccgc gccgcttgaa
     9181 actcccaaaa catacgggtc cgccaaggga ttgcgcagca aagcctggaa acttgcgccc
     9241 gcgacggaaa gcgcggctcc caccaaaatg cccagcaaaa tgcgcggcag ccgcaaatca
     9301 aaaacaatca gcctatattc agtgggcaat tcatttgcgc ggcccgacgc gatgcggcca
     9361 agatccaaaa ccagatccgt gaccgagatc ggcacagcgc ccaacttgag cgcaagcacc
     9421 acgctcccca gtaacaatgc ggtaaggcca ccgcaagtcc acaataatcg cctgagagtt
     9481 agtgggtgca aatgcaagcc tcttcgatat ccgaattcat tgtggcgata tctaaatcac
     9541 gagacggcat cgcgcgcgag gcaaatgctt ccgggtgaag cgcgcgcgca agttgctcga
     9601 tggcgtccac cattcgcggc gcaggccgat tgatcgcgtc gctgatcaca atgatgttac
     9661 cctgctgtat tgccgtaaga cttttccagc cgggacgagt gcgaagcgtt tcaatatcgt
     9721 gccgcgcatc tcccgtatgt gcgctggcaa acacaataaa ttccggttga agcttcacca
     9781 tttcttccat gctcacgtgc ggccactcaa cgcgggtatc gaccacggag cgtccacccg
     9841 cgcggcgcag cgcgtcggca ataaaagtat cgcttccaac ggaaatcagc ggatcggtcc
     9901 acacgacaaa caggacgcgc cgttgcgctc cgccggaaat tcgacgatcc agatcggcca
     9961 ggcgcgcgcg caaatttccc gtcagcatgt tcgcagattt ttccacgccg atcgcgctac
    10021 cgatgtgttc cacggatgta atcatcccat ccaccgaatg cggatccgtc acgtagaccg
    10081 gcaaaccaat atgatccagg gcattcaccg attcgcggcg gttaatcgac tttgacgcca
    10141 gaataagatc tggcatgaga gatacgattt cttccaggtt tggattcacg ggcccgccga
    10201 tatgcggctt ctgcaatgct tccgccgggt aatcgcaaaa atcggtatca cctgccagat
    10261 gatttccttc acccagtgcg aatacgatct ccgtaaggtt tggtgcgaga gaaactatgc
    10321 gatctacttc ttgcggggcc cgcacgcgcc tgccaacttc gtccatcagt tcacgcgccg
    10381 cgggagtcgc tgcgcgcgaa gtgtccgttt gcccggggct cggttgccct gcggaggcac
    10441 taccgcaggg gataacgagg agcactaaaa ttgcagcccg tacaattgcg cggccgcgct
    10501 tccaggattt cacgctcaat gtttcagctg cgcgaattgt gtttttcatt cccatcgatt
    10561 caatgcacag ccgcaaaata aaaatgcctc gcgagaaaca cgaggcgcat aatgccacac
    10621 gctttctcgc gaagggtgtg ggctgcggcg gtctgagagg gtgtcctgac ttacggcgtt
    10681 tcgccgatca cagtggcggg accgtggccg attttcacgg ccttccccac ttcccaaacg
    10741 tgctaaatgt caaaagcagt ctatggcgct gcttgacgag tgtcaacaag ggccattttt
    10801 ttcgaggcgc ggcaatttga atcgcacacg caaagaatcg ccagcccaaa aaacgggtgt
    10861 cctctacgaa agcaatatca aagcattggg gtattcgtcg aggcatttcc gtcccgttca
    10921 cggagaacgt tctcggtaaa ccgcgttgtt caggacagag agggcactcg gtgttaggaa
    10981 gaagaaggaa ccttgttgtt ctcggccgtt aaataagaat ccgctatcgt tttttttcaa
    11041 caccctgtta gactgccgcg ggggtaatca tcgtggtttc tgcgctattg gtcggacata
    11101 tttttggttt ggtgttttgg gtaagcggct tgctcgttac gtccatggcg ctcacgcgcc
    11161 atacgcaaga aacatcggcg gacgcgaagc aagcgctagc gcgccttgag cgcatctact
    11221 tgcgcggtct tgcggatccc ggtgcgctgc tcaccattct cgcgggcctc ggcttgatct
    11281 cgaccaacgc ttcctactac atgcacgctc cgtggctgca tatcaagctt actttcgttt
    11341 tactcctcat cggcctgcat ggattggtag cggtgcgaac taaaaaattt tccaccggaa
    11401 aaatttccat gcagcgcagt gaagcgcgca tgctggtagc agccgtgtta ttcctttttg
    11461 cgtctatcct tgtggcgact ctcgtaggcc aagtttattt gacttgatcg cgtgcggtgt
    11521 tgccagtgac gcgactggaa attaacgcgg ggaaaaaatc aggtacggga caattcgcgc
    11581 tcgtccggtt cttgttccca atggacatca tcgatccacc accgcccgtc ggcgcgaact
    11641 agcgtgaatc gcgcgcggta atccttgtca tccgcgctat gcaaataaat ttccagcgtc
    11701 actccgctcg tgctttgcgc cggaatttcc gaccgcacca cgtccgcatg aaacatccgc
    11761 cagtgcgtaa taaacaattc atgccaggta tcaaacgaaa cttgtccgcg gtaccgcggt
    11821 gaaaacatat cgtacgcggg gcgcaattct ccgtggtcga gggcgcgaag ctgcgcacga
    11881 actacttcct gtggaccgct tgaaaaaatg ccgaacggtg agtccacgcg caccagaatc
    11941 cacgatgaca aaataaatgc agccagcgtt agaccgagca gcagcaataa attattgcga
    12001 aaaccgcgct ttcgctccgc cgcatccgcg tacatggcaa tcacatccgc gtgccggtcc
    12061 tctggcgatt ttagctgcgg ctcaaaagcc ggagttagat cgagtagggg ctcagcaaac
    12121 ggcgttgctt cagggggcac accagtatca aaaggctctt cagagggaat tgaatcgggt
    12181 gtgtttagag gatcgtccat acctatctcc cgcgcggcgg ggagacgctt tcggcctacc
    12241 taacgattct tccactgtgg cttgcgtttt tcaagcagcg cgcgcaatcc ttcctgcgaa
    12301 tcttcgaggc ggtacaattc attcaggaaa atcttcatcg agttttgcag cgagtcacgc
    12361 agggacatac ccattccgcc aaggactgcg cgcttggcca tacccagcac ggctccggat
    12421 tgcgctgtaa tctttccgat catttcattc accgcagttt gcaactgaac ctcggggacc
    12481 aggcgattca ccaggcccaa atcgcgtgcg cgttcggcgg aaatggcttc tccagtcagc
    12541 acaagctcga gagcccgttt cggcccaaca atatgcggaa acatggtgga agccagggga
    12601 ggaaacatgc caatcgtgat ttccggcaga gcgaatcgcg cgcgtggcgt ggcaactacc
    12661 aaatctccat acgcgacaag ctccgcgcct ccgccaatcg ctgcgccatc gacgatgacc
    12721 aacacaggct gcggcgcttc caccatggcg atgcacgcgg aatgaaaagc atccagcatc
    12781 gaaaaggctc tctccgccgt gtactcgcca atatccacgc cggcacaaaa gagttttcca
    12841 gcggcatcca gaataatcat ctttaccgcg ccattttcgg cgatcaatcc aacgccgtcc
    12901 gccagttcgc gcagcatctg ctcatttaat aaattgtgtt ccgggcgttg caatgtgagg
    12961 cgtgcgacag cgccttccac ctgaaagcgg ataaaagaga agtcggcggg cgtacggcgc
    13021 gcaggctttg cgaacgaagc ctcggtctgg accaagtctg tcatttttca ctccaattta
    13081 tgctagcacg accggagtcg agcaaaaatg cggctgcaac gggggcgaag ttaagagtta
    13141 acagttcaga gttgtgagtt gtcaggctcc tgttcattgc tctcaactct caactattaa
    13201 ctctcaactg tcttcatgta ttctattgcg catgcatgaa cttggatatt ttcgcgagca
    13261 cctcgaagaa tttgaacaaa tggccgccaa tcgcggcgta aaaatcgact ttgcccactt
    13321 tcgcaccatc gaccaggagc gccgcgaacg aattaccttt gccgagcgct taaaggccca
    13381 gcgcaataaa gcgagcgagg aaattccgcg caagaaaaag gccggcgaag atgtctccgc
    13441 gctgctggcc gagatgaaac aagtttctga agaaattaag caggccgacg agcgcctcgc
    13501 ggagctagac gcgcaacttg cgttcttcat gttgaacgtg cctaatcttc tacacgcttc
    13561 agttccggtc gggcgcgatg ccgcggcaaa tgtggaagca cgccgctggg gcgctccgcc
    13621 aaaattcgat ttcaagccgc gtccgcattg ggaaatcgcc gaaagtgcag gcattctcga
    13681 tttccagcgc gcggcaaaaa tgagcggcgc gcgtttcgca atctatcgcg gcctcggcgc
    13741 gaaactcgag cgcgctcttg ctgcgttttt tctggaaacg cacgcggcca acggatacac
    13801 cgagattctt ccaccgtttc tcgttaacac tgcggctctg attggcgtgg gccagttgcc
    13861 gaagttcaaa gcggacatgt tccatctcga aggcacggac ctttggctca cgccgacatc
    13921 ggaagtggaa ctcactagct tctaccgcga cgagacgctc gacgccgatc agctgcccgt
    13981 caaactttgc gcgtggaccg catgcttccg ttccgaagcc ggtgcagcag gaaaggatac
    14041 acgcggcttg aagcgtcagc accagttcca aaaagtggaa atgtttaaat tcacacgccc
    14101 ggaacaaagt tacgacgaac tcgaatcgct cgtcgccaac gccgaactaa ttctccaaaa
    14161 gctcggtttg cattaccgcg tcgttggcct ttgcacgggt gacatgggtt ttgcgtcatc
    14221 gaaaacgtat gacatcgaag tctggctgcc ctcggccaac gaattcatgg aaatttcttc
    14281 gtgttccaac acggaagcgt ttcaggcgcg ccgctccggc attcgcttca agcccaaggg
    14341 cggcaaaagc gaattcgtgc acacactgaa cgggtcgggg ctggcagtgg gccgcacttg
    14401 gcttgcgatc atggaaaatt atcagcaggc ggacggttcc gtggtgatcc cggaagtctt
    14461 gcgaccctat ttagggacgg acagaatacc tgcgaaatct tgagcctgtt cgcgaaacgg
    14521 agttggcagg gtcgcaaccc taacggtctg gtattttgcc gacgtcactt catgtccttc
    14581 gctataaatt gcccctccca gccaagtagc tcgtttagac cccaaggcta ggtgtaagtt
    14641 attcattatg agctacttaa ttgggatatt aaatccaaat ctaaagtaag ctcagtgttt
    14701 ccaaagcatc ctcgccgaaa ataaccgggt tctttggttg acaccgcttt tccgtgttga
    14761 ctacactccc cttattgcgc ggacatcaat cgaacggtcc gcacaagggg taattattca
    14821 agggcttcgg cacgcctggt ccttcgtatc gggaaacgaa gaacattgcg cggcaggccg
    14881 gggcgtagtg gccgctagga agcgtcgagc gggtgagttt acaacagcaa atcgatcggt
    14941 gggtaagtcg agggctggcg tggatgatgc gcacgcttct ggccaaggcg ctgggaacgt
    15001 cgggtactcc acccatgcct caaccccacg agacgcttac cccggtcgcg ctgcaacgcg
    15061 atccgtattg tggcacctat gtctcacccg aaatttcatt ccccttggag caatcgggcc
    15121 aagtgttgca cttctgttct gccgaatgtc gtgcgcggta tcagtcgaat gcaaagcgcg
    15181 cggcgagtgc atagtcgcga tactcaagga gagaatcgtc catggccacg gtcgtaaaaa
    15241 ccgttcctca accccagcct attctgccgc gaatggcacc gcgcgaggag ctgaatccat
    15301 tccgtatcgc ccagattcag tttgatatgg ccgcggaata cttgaagctc gatcccggac
    15361 tgcgtcagat tctgcgcacc ccgaagcgag tcatggaagt tgctgtcccc gtaaagatgg
    15421 acaatggcca gactaaggta tttacgggat tccgcgtgca acacaatgtg tcgcgcggtc
    15481 cggccaaagg cggattgcgc tatcacccca acgtaacgct cgacgaagtg aaggcgcttg
    15541 ccgcctggat gacctggaaa acggcgacgg tgaacctgcc ttacggcgga gccaagggcg
    15601 gcgtcatctg cgatccgaaa cacatgtcca agggtgagct tgagcgcatg acgcgccgct
    15661 acgccgcgga aattcagccg atcatcgggc cggaaattga tattcccgcg ccggacgttt
    15721 acaccgattc gcaaactatg gcctggatca tggacacata ctccatggca gcggggcatg
    15781 cttctccggg tgtggttacc ggtaaacccg tttcgattgg cggctcggaa ggccgctcgg
    15841 acgccaccgg gcgtggagtt ctatttgtcg tcgaagaagc ttgcaaggta aagaaaatta
    15901 atttgcgcgg cgcttcagtt gccattcagg gatttggcaa cgtggggtca gccgtggcgc
    15961 gtctgtttgc cgagaaaaaa gcgaagattg ttgcgatcag cgattcccgc ggcggcgtgc
    16021 acaatccccg cggtatcgat ccgctgcgcg cgatgcgata caaggagcgc gcgggaactg
    16081 tggtaggcat gccaggtgct tcgcgcatct cgaacgacga acttctggcc ctgaagtgcg
    16141 acattctgat tccagcagcg ctcgaaaacg caattacgtt gcacaatgcc gaccaaatca
    16201 aggcaaagat cgtcgcggaa gcaggaaacg gtcccaccac gcctcacgcg gacgaaattc
    16261 tttcgcgcaa aggtattttc gtgattccgg atattcttgc gaacgccggc ggcgtcactg
    16321 tcagctattt tgagtgggcg caggatttgc agggattttt ctggcaagtg caggaagtga
    16381 attccaaact tgagttcgtc atgcggcgcg cgtttaacga tgtgcacgaa accatgcgca
    16441 agtttcacgt ttatccccgt gcggcagcct atatcctcgc cgtcgggcgc gttgccgatg
    16501 ccactttggt acgcggtctg ttcccataaa gcgcttcacg catttttatt ttgcgttttc
    16561 cgattttttt cttctcctat acatggatac atgaaaattt ctattgcgcg tgggcggcca
    16621 cgcgcgtaca tttcgaattg cctgcttgca tgaagttttt tgcggccggc ccacttgggt
    16681 ggtgcgagtt ttttcgcgga ggggctcgaa aaattgtcca atcatgcgaa cgtggctccc
    16741 ccgatcgtgc tgacgatcgc gggcttcgat ccatccgccg gggcgggaat cgcggcagac
    16801 ataaagacat ttgcagcaca caattgttac ggcgttgcgg ctgtcaccgc gctcaccgtg
    16861 caatccacgc aaggcgttaa atccgtgcat gatactccgg ccgcggaatt gcgcgctcaa
    16921 cttgatatgc ttctggaaga cattaaattt tccgccatta aaatcggcat gttggggcat
    16981 cgcgggaatg ctgtagtggt cgcggaattt ttggatcgcg ccggcgttgc aaatgtcgtg
    17041 ctcgatccgg tgatgaaatc cacttctggg catgccgacc tgatcgatgc cggcggaatt
    17101 aagtgtatca gcgaggaatt gttgaaacgc gcaacggtta tcacgcccaa tattgccgaa
    17161 gcggaaattc tttcgggcat ttcgatcaag gatgccgcct ccatggaagg cgcggcccgc
    17221 aaaattgttg agcgtggagc tcgcgccgtg gtcgtcaagg gcggccacat ggacaaagcg
    17281 gtcgatgttc tttttgatgg cacggaagtg ctccggtttg gcggcgatca cttgaaaaat
    17341 gaaaatacgc acggatcagg gtgcacattt gcgtcagcga ttgccgcgca gcttgccagc
    17401 ggtcgtcccc tgcgagaatc cgtgctgctc gccaaagcct atgtgaccaa ggccatcgaa
    17461 aaaggttttt cgatcggcaa aggccccggc ccgctcgatc atttttaccg cactcgcacc
    17521 gagccgccca cgcgcggcgt ccacgaagtc ccgcaacacg gtatgcaccc cgccgctgaa
    17581 cctggcgcgc actaaaagca aaaagcgttg gaaccacaga tgcacacaga caaacacgga
    17641 taaaaccaaa aaataagatt gaaataaaaa accgaggatt ttaatctgcc tcgcttattt
    17701 tatttgcttt cgcttttatc cgtgtttatc cgtgcgtctc tgtggctatc cttaatgtag
    17761 accatgcggc ccgcaacaat cgtcgccaca gggccgccgc ggaattcccg gccatcgaat
    17821 ggggagttct tagacttgct aacagattct tttgcacgga aagtccattt ctgttcggtt
    17881 gaaaaaatcg ttaggtcgcc tggctcattt tcagcgatgc ggcgctgctt acctagtacg
    17941 cgcgccggtc ctatcgtgaa cagttccacc aggcgcatca gcgaaagttt ccccgcatgc
    18001 acaagttcaa ggcccaagcc aatcgctgtc tcgaaaccgg tcacgccaaa tggagcgcgg
    18061 tcaaactcta cttcttttag cgcagcttcg tgtggtgcgt gatccgttgc aatggcgtcg
    18121 accgtcccat ccgcaagccc ggcaatcagc gcatcgcgat cttcgcgcga agcgagcggc
    18181 ggattcatct tgtagcgcgt gtcatactgc acgtcttcat cggtcagcgt gaagtgatgt
    18241 ggcgtcactt cgcaggtgac gtgcagtcct ttcgcttttg ccgagcgcac cagttccaga
    18301 gaagagcgtg ccgaaaga
//
LOCUS       DASZMB010000069         7003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1176511, whole genome shotgun sequence.
ACCESSION   DASZMB010000069 DASZMB010000000
VERSION     DASZMB010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7003
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1176511"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="VG272_03235"
     CDS             <1..1322
                     /locus_tag="VG272_03235"
                     /inference="COORDINATES: protein motif:HMM:NF011656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF12723.1"
                     /translation="DRKQPGPMSTQTKGSKVGNVIRISSGNFLEMYDFMVYAYYTSYI
                     AREIFPSSNAYASLMMTLGTFGAGYLMRPLGAVVLGAYVDRHGRRAGLIFTLALMAVG
                     TMVIAFTPPYRTIGIIAPIVVLLGRLLQGFSAGVGVGGASIYLSEMATPGHRGFYCAW
                     QSASQQVAVVFAALLGVGLSIVMPPERMSAWGWRVPFLIGCMIVPLLFWLRKSLDETE
                     AFLAQKRHPHLNEVLASLATNWKIVTLGVLFSTMTTVCFYLITAYTPTYGIEVLHLTS
                     RQSLMVTFCVGLSNFFWLPTGGAISDVVGRRPMLIGVSLAAILTSYPAMLWLVSNPST
                     TRLLIVELWLSMFFGVYNGAMIPHLAEIMPPEIRTSGFSLAFSLATAIFGGFTPAICT
                     YLIHETGNRAMPALWLSFAALCGLVAAVAGGWHRVPVRALKSEVSAN"
     gene            complement(1368..1859)
                     /locus_tag="VG272_03240"
     CDS             complement(1368..1859)
                     /locus_tag="VG272_03240"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HWF12724.1"
                     /translation="MHFVFTICQWIENTGIGGGIRESTWLFPVIETVHIFGIVMLVGA
                     TSVLDLRLLGWTFKGRSVSKMAWRFLPWAWVGFTIQLLTGVLLFASEATKMYENLGFQ
                     IKMVLIVVAGLNALLFHSIAYKSVDKWEYDAVGPLGARAAGLISIVLWFGIVAAGRWI
                     AYV"
     gene            2396..2683
                     /locus_tag="VG272_03245"
     CDS             2396..2683
                     /locus_tag="VG272_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWF12725.1"
                     /translation="MSSDSVEEGKRLYGATDCALCHGKDGDGKGLEAKDVNMNTHDWR
                     KPQNLEHFTDGELAYLILKGKGRMPAYDGRETPDQVWRLVNYIRSFPLTGK"
     gene            2939..5074
                     /locus_tag="VG272_03250"
     CDS             2939..5074
                     /locus_tag="VG272_03250"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HWF12726.1"
                     /translation="MSIFTKRIVTGVIKVFEAPISRNSILRLSSCAAILLFVLLASIQ
                     LLKASPQSGNAKVSGAADHGADLTILDKTCKPCEAFYHYADGAWLTKNPVPPAYPSWS
                     RFSELAERNRELLHQILEQAAVTKSAAGSNEQKIGDFYGSCMDEKQVNSAGTKPLDSE
                     IARINAIQNVPGLQAEIARLQGLGLRAVFQFGSTQDSKNSTEVIGGADQGGLGLPDRD
                     YYTKTDEKSQQLRQQYSEHVSKMLALAGDDETRAVSEAKSILDMETKLAEASMTRVDR
                     RDPDKTYHRMNREQLKALTPNWSWEEYFQEIGYTNINAVDISAPKFFQAMSDEMKATP
                     LVTWKIYLRWHVLNSSAPYLSQPFVDENFNFNGQILLGTKELLPRWKRCVSATDRQLG
                     EALGQIYTEKYFPPEAKARALDMVNHLIDALRDDLQTLPWMSAATRKEALTKLGAFTR
                     KIGYPDKWRDYSAYAVDRGPYVLNQMRGSHFEFVRDLNKIGKPVDRGEWGMSPPTVNA
                     YYNPQMNEIVFPAGIMQPPFFDPKADDASNYGAIGAVIGHEMTHAFDDSGRKFDAQGN
                     LRDWWTPEDEKNFNERAACVEKQFSDFVVQDDVHENGKLVVGESIADLGGLNLAYRAL
                     QKAEQGKKSALIGGLTPDQRFYIAFAQIWASNDRPEYERLIVNTDPHPLGRFRAIASP
                     SNMPEFAHAFDCKPGDALARPAAVRCQIW"
     gene            complement(5277..6029)
                     /locus_tag="VG272_03255"
     CDS             complement(5277..6029)
                     /locus_tag="VG272_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF12727.1"
                     /translation="MSRILVVEDEAHLAEGLRFNLEQEGYSVRVAGTGEDALQLLLKD
                     REPFDALVLDVMLPGEDGFAVARAMREARNYIPILMLTARSRPEDVLQGFESGADDYL
                     PKPFILSIFIARVGSLLRRKTWERVDPKSPAKPAASETESVETFTFGDKVADFANLTL
                     KVSNETVQLTLMEAELLRYLIENAERPVSRKAILENVWNLQEDTDTRAIDNFIVRLRK
                     YIEKEPSDPKHLVTVRGLGYKFIPFPDKKAKK"
     gene            complement(6026..6931)
                     /locus_tag="VG272_03260"
     CDS             complement(6026..6931)
                     /locus_tag="VG272_03260"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWF12728.1"
                     /translation="MRITGRAKKIAFFVTLGACLVGLAIALNVTWIVLNWRAAVPLVL
                     GIIFFGLIIAGLVVYTVFLVREIHKNEQQDSFLNAVTHELKTPIASIRLYLETLQTRQ
                     LTEAQHQDFYRIMLEDTDRLLGTVEQILKAGEARHASSKKNWEQVDFSGIVAETVELT
                     RLRHHFAPEVLRFAGPAAEKIYLRGNPQELRTAVFNLVENAVKYSGELKEIEVDLLTP
                     DIDTILLQVRDKGVGIPPAELKRIFKRFYRVTSSATGRVKGTGLGLFIVRSIARRHGG
                     DAFAQSEGTGRGSTFTLRLPRAYPV"
BASE COUNT         1594 a         1940 c         1873 g         1596 t
ORIGIN      
        1 aagatagaaa acaaccaggt cctatgagca cacaaacgaa gggctcaaaa gtcggcaatg
       61 taatacgcat ctccagcggt aattttctgg agatgtacga tttcatggtg tacgcgtact
      121 acacttcgta catcgcgcgc gaaatcttcc cctccagcaa cgcatacgct tctttgatga
      181 tgacgctggg aacgtttggc gcgggatacc tgatgcgacc actcggtgcc gtcgttctgg
      241 gcgcgtacgt cgaccggcac ggccggcgcg ccggtctaat tttcactctg gcgttgatgg
      301 ccgtcggcac gatggtcatc gcgttcactc cgccgtaccg gacgatcggg attatcgctc
      361 ccatcgtcgt gctgctgggc cgtttgctgc agggtttttc agctggcgtc ggagtgggcg
      421 gtgcatccat ctatctttct gaaatggcta cgccaggcca ccggggattt tattgcgcat
      481 ggcaatcggc cagtcaacaa gtggccgtgg ttttcgccgc cctcttgggt gtgggactca
      541 gcatcgtcat gccgcctgag cgcatgtctg cttggggatg gcgcgtgccg tttttaatcg
      601 gctgcatgat tgtcccgctg ctgttctggc tacgcaaatc gttggacgag acagaagcat
      661 ttctggcgca gaaacgccat ccgcatctga acgaagtcct tgcctcgctc gcgacgaact
      721 ggaaaattgt tacgctcggc gttttgttct ctaccatgac gacggtttgc ttttatctga
      781 tcaccgcata tacgccgacg tacgggattg aagttctgca tctcaccagc cgccagagcc
      841 tcatggttac attctgcgtc ggcttatcta attttttctg gctccccact ggcggtgcga
      901 tctcggacgt tgtcggaagg cggcccatgt tgatcggtgt ttcgctggcc gctattttga
      961 cttcgtatcc cgcgatgttg tggctggtca gcaacccctc caccacacgg ctgctgatcg
     1021 ttgaattgtg gctatccatg tttttcggcg tttacaatgg cgcgatgatt ccgcacctcg
     1081 cggagattat gccgccggaa attcgtacat ctgggttttc gctggcgttc agtttggcga
     1141 cggcgatctt tggcggattt actcccgcga tctgcacgta cctgattcac gaaacaggga
     1201 atcgcgcgat gcctgcgctt tggctatcgt ttgcggcatt gtgcggatta gtggcggctg
     1261 tggcaggcgg gtggcacaga gtgcctgtgc gggcgctgaa gtctgaagtt tctgcaaact
     1321 aaagcaacac aggcttcagc ctgtgctact taagtcgaga cccgccgtta gacgtaggcg
     1381 atccagcggc ctgcggcaac aattccaaac cacagcacaa tggaaatcag gccggccgcg
     1441 cgcgcgccaa gcggaccgac agcgtcgtac tcccacttgt cgacgctctt gtaggcaatg
     1501 gagtgaaaga gaagcgcgtt taagccggcg acaacgatca gcaccatctt gatctgaaac
     1561 ccgagatttt catacatctt ggtggcttcc gatgcgaaca gcagaactcc ggtgagcaat
     1621 tgaatggtga accccaccca cgcccacggc agaaatcgcc aagccatctt ggacacggac
     1681 ctgcctttga acgtccagcc caacaggcgg agatccagca ccgaagtcgc ccctacaagc
     1741 atgacgattc caaaaatatg aaccgtttcg atcacgggaa atagccacgt ggattcgcgg
     1801 atgccgccgc cgatccctgt attttcaatc cactgacaaa ttgtaaaaac gaaatgcatc
     1861 ttgctggccc tcacattcca tcggatagct tcattctgct taagctcgca tgaaaacaca
     1921 aagaatattt cgcgtcgcag ctcacgtgct ggcgttcgtt cgcaaggcag agaacgttag
     1981 atgcgaaagc gacctgcgcc gtttttcaac ttttatggcg cttgccacag ctagcgaacg
     2041 gaaacgttct tttactgtaa aacgggctgt cgcacaagaa catttctatt gcgcatccca
     2101 atccgatggc caacccagcc gatcgacagc ttcccacact tacgaaaaac aaattgctct
     2161 gcaaatttgg tttcgctgta cgactctgct taaaaaatat aaacttcgtt tccagtcgtg
     2221 tgcttaaaga ccgctaaaca cagtaaggtt caagtgtccg ggccggaggg gaaaaatggg
     2281 gcgtgccgca attttgtgcg taatggtgtg cagtgtgttg ttcgggtgtg ggggtaagtc
     2341 ggttgaacag gcgaaagttc cccaggaaga aattaaccgg gcgaatcccg tacccatgag
     2401 ttcggattcc gtcgaggaag gcaagcgttt atatggcgcg acagattgcg cgctctgcca
     2461 cggaaaagat ggcgacggca agggccttga ggccaaagat gtgaacatga atacgcacga
     2521 ctggcgaaaa ccgcaaaacc tggagcattt cacggatgga gaactcgcct acctgattct
     2581 gaaaggcaaa ggccgcatgc ctgcttacga cgggcgggaa acacctgacc aggtgtggag
     2641 gcttgtaaat tacattcgct cgtttccgtt aaccggaaaa tagtagggga gttccttcgg
     2701 tagttggcac gaactcgttc gaggtgctga ttcatctaaa cccggcctct ggtccctcgt
     2761 tttcgctcgt gacaatccag ccaggctaca ccgacgggct cctcggcggt gatcgatgca
     2821 gcctccggtt tcctccaaca ttttcttagt gtcctctgtt aaagcttttg cacttctgtt
     2881 gcggaatctg ccatcctata gagagccgaa tgcccaacgt ttgattaagg gagtagctgt
     2941 gtcaatcttc acgaaacgca ttgtaacggg agtgatcaaa gtttttgagg cgcccatctc
     3001 gcgaaattct atcctccgct tgtctagctg cgctgctatt ttacttttcg tgttacttgc
     3061 ctctatacag ctgctgaagg cttcccccca gtcgggtaac gctaaagttt ccggagcggc
     3121 agatcacggc gcggacctca caatcctcga taagacgtgc aagccttgcg aagctttcta
     3181 tcattacgcc gatggtgcgt ggctgaccaa aaatccagtg ccgcccgcct atcccagctg
     3241 gagccgcttc agcgaactcg cggaacgtaa tcgcgaattg cttcaccaga ttctggaaca
     3301 agctgccgta accaagtcgg ctgccggatc gaatgagcaa aagatcggcg atttttatgg
     3361 cagttgcatg gacgaaaagc aggtgaactc cgcgggaacg aaaccgcttg attcggaaat
     3421 cgcgcgcatt aacgcgattc agaatgtgcc agggttgcaa gctgaaattg cgcgtctgca
     3481 gggattgggg ctgcgcgctg ttttccaatt tggctccacg caggactcta aaaacagtac
     3541 cgaggtgatt ggtggcgccg atcagggcgg tctcggtttg ccggaccgcg attactacac
     3601 caagaccgac gagaaatcgc agcagttgcg gcagcaatat tccgagcacg tgagcaaaat
     3661 gctggcgctc gcgggagatg acgaaacaag agctgtgtcc gaagccaaat ccatcctcga
     3721 tatggaaacc aagctggcgg aagcttccat gacgcgcgtg gatcgccgcg atccggacaa
     3781 aacgtatcac cgcatgaatc gagaacaatt aaaggcgctc acgcccaatt ggtcatggga
     3841 ggaatatttt caggaaatcg gctatacgaa tatcaatgct gtcgatattt ccgcgccgaa
     3901 attcttccag gccatgagcg acgagatgaa agccactccg ctcgtcactt ggaaaatata
     3961 tctgcgctgg catgtattga attcctcagc gccctatttg tcccagccgt ttgtcgacga
     4021 aaattttaat ttcaacggcc aaattctgct gggcacgaaa gaactgctgc cgcgctggaa
     4081 gcgttgcgtc tccgctacag accggcagtt gggcgaggcg ctcggacaga tttacaccga
     4141 aaaatatttt ccgcccgaag caaaagcccg cgcgctggac atggtgaatc acctgatcga
     4201 tgcactgcgc gatgatttgc agactctgcc ttggatgagt gcggcgacgc gcaaagaggc
     4261 cctgacaaag ttgggcgcat tcacacgcaa gatcgggtat ccggacaagt ggcgcgatta
     4321 ttcggcctac gctgtggacc ggggtccgta cgtgctgaat caaatgcgcg gttcgcactt
     4381 tgaattcgtg cgtgacctga acaaaatcgg caagcccgtg gaccgcggcg aatggggcat
     4441 gtctccgccc acagtaaacg cttattacaa tcctcaaatg aacgaaattg tttttccggc
     4501 aggcattatg cagcctccct ttttcgatcc caaggctgat gacgcttcca attacggcgc
     4561 gatcggcgct gtaatcggcc atgaaatgac gcatgccttc gacgactcag gcagaaagtt
     4621 tgacgcgcaa ggaaacttgc gggactggtg gacgccggaa gacgagaaaa atttcaacga
     4681 gcgtgccgcg tgcgtcgaaa agcagttcag cgatttcgtg gtgcaggatg atgtccacga
     4741 aaacggcaag ttggtggtgg gcgaaagcat cgccgacctc ggcgggctga atcttgccta
     4801 ccgcgccctg caaaaagcgg agcaggggaa aaagtcggcg ttgatcggcg gcctaactcc
     4861 tgaccagcgc ttttacatcg ccttcgcgca aatctgggcg tcgaacgatc gtccggaata
     4921 cgagcgatta attgtgaata ccgatcctca cccgctcggc cggttccgcg cgattgcctc
     4981 tccctcaaac atgccggaat tcgctcacgc attcgattgc aagcccggcg acgcattggc
     5041 gcgacccgca gccgttcgat gccaaatctg gtagacggtc aggctgacga ttcatcacca
     5101 agtagcacag ccactcttgg ctgtgtgggc ttcgcaaaac ccgcacaagc aaggttgcct
     5161 gtgctactca aagccacaag ggctgcctta atgcgttcgc tgcacaatcc attgccacat
     5221 cgggtccgct ccagcaattc attgtgtgcc ggacgagact cccctatgcg actgctctat
     5281 ttctttgctt tcttgtccgg gaacggaatg aatttgtagc caagaccccg cacggtcacc
     5341 aggtgcttcg ggtctgaagg ttccttctca atatattttc gaaggcggac aataaagttg
     5401 tcgatggcgc gggtatccgt atcctcctgc aggttccaca cattttcgag aatcgctttt
     5461 cgggacaccg gacgttccgc gttttcgatc agataacgca acaattccgc ttccatcaag
     5521 gtcagctgta ccgtttcatt gctcaccttt agcgtcagat tcgcaaagtc cgctacctta
     5581 tccccaaacg taaacgtctc gacggattcc gtttcgctcg ccgcaggctt agccggggat
     5641 tttggatcga cgcgctccca ggtcttgcgc ctcaaaaggc tgcccacgcg cgcaataaaa
     5701 atggagagaa taaatggctt gggcaggtag tcatccgcgc cggattcaaa accctgcaac
     5761 acatcctcag gacgcgagcg cgccgtgagc ataagtatcg gaatataatt tcgggcttcg
     5821 cgcatcgcgc gagcaacggc gaagccatcc tcgccgggca gcatgacgtc caacacgagc
     5881 gcgtcaaacg gctccctgtc tttcaggagc agttgcagcg cgtcttcgcc cgtgcccgct
     5941 acccggaccg agtagccttc ctgctccaga ttgaatcgca gtccttcggc aaggtgcgct
     6001 tcgtcctcca ccacgagaat ccggctcata cgggatacgc tctcgggagg cgcaatgtaa
     6061 aggtgcttcc gcgccccgtg ccttcgctct gcgcgaatgc atcgccgcca tgccgacgcg
     6121 cgatagacct gacgatgaac aatccgagtc ccgtgccctt gacgcggccc gtcgctgagc
     6181 ttgtcacgcg gtaaaaacgt ttaaaaatgc gcttcaattc ggcgggagga atgccaaccc
     6241 ccttatcgcg tacctgcagc aggatggtgt caatatccgg cgtgagcaaa tccacttcaa
     6301 tttcctttag ctcgcccgaa tatttcactg cgttttcgac gaggttaaac acagcggtcc
     6361 gcagttcctg gggattcccg cgcagatata ttttttccgc cgcgggtccg gcaaaccgca
     6421 gcacctcggg agcaaaatga tggcgtaaac gggtgagttc caccgtttcc gccacgattc
     6481 cggaaaaatc cacctgctcc caattctttt tgctgctcgc gtggcgcgct tcgcccgctt
     6541 ttaaaatttg ctccactgtc cccaacagcc ggtctgtgtc ctcgagcata atgcgataga
     6601 aatcctgatg ctgggcttcg gtcaattgcc gcgtctgcag cgtttcaaga tacaaacgga
     6661 tggaggcgat gggagtttta agttcgtgcg tgacggcgtt caagaaactg tcctgctgtt
     6721 catttttgtg aatttcgcgc accagaaaaa cggtgtacac cacgagaccg gcgataatca
     6781 gtccgaaaaa aatgatcccc agcaccagcg gtacagctgc gcgccaattc agcacgatcc
     6841 aggtaacgtt cagcgcgatc gccagcccca cgaggcaagc gcccagcgtc acgaagaatg
     6901 caatcttctt ggctcgtcca gtaatgcgca tcacaggttc tttctatctg aacatagtaa
     6961 tcgagatgcc ggagaaaaaa aacgcagggg tgttagtgcg ggg
//
LOCUS       DASZMB010000070        10819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4205210, whole genome shotgun sequence.
ACCESSION   DASZMB010000070 DASZMB010000000
VERSION     DASZMB010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 10819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10819
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4205210"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(326..685)
                     /locus_tag="VG272_03265"
     CDS             complement(326..685)
                     /locus_tag="VG272_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12729.1"
                     /translation="MTAALIATQFPDTIMLSACVALTLAAVIFVFWIEPDPGDSAPHR
                     SQLDQLMERRDSIYDNLRDLKFENRAGKYAEQDYEQMRDALETEAAQVLLEIERVTGN
                     QAPIPRRDPVNAANPRQ"
     gene            complement(698..1216)
                     /locus_tag="VG272_03270"
     CDS             complement(698..1216)
                     /locus_tag="VG272_03270"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HWF12730.1"
                     /translation="MTNRRHFAFICAAFVALATALVPTHVSAQKTDRAKSLGTKLMCM
                     CGCGQILTQCNHINCPSSGPMLKELDAHVAKGESDDLIVQDFVQEYGEKVLSAPPTKG
                     FNSIAWYIPGVAFAIGLGIVITLIRLWRQRDVARLATASSAPPVAPTSASELRDVQLE
                     RARQQADRETEE"
     gene            complement(1209..3287)
                     /locus_tag="VG272_03275"
     CDS             complement(1209..3287)
                     /locus_tag="VG272_03275"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HWF12731.1"
                     /translation="MDLFGAYSLLLAFAFAVYAIFGGIAAIITRRPLLIKSARNAGFA
                     VCVLIWIGTCSLWYLFFTNNYSLAYVAAHSNRTLSPYYKFSALWSGQEGSLLFWSFLL
                     SIYVFSALFTYRGKHPELMPYVGVVLAGVQIFFLTMNNFVASPYGVFVGHGAGGILHL
                     ATRADGSGLNPLLQYPEMAIHPPTLYSGYTGFTIPFAFALAALLGRYPGEKWIHLTRR
                     WTMIAWAFQSAGILLGAHWAYAVLGWGGYWGWDPVENASLMPWLTGTAFLHSVIMQEK
                     RGMMRVWNVWLVFLTFMLCILGTSLTRSGAVNSVHAFAESNIGHWFWGFLLTIVIVCL
                     AAYFKDRDYLRSDNQLDSMLSRESSFLFNNLILLVACVAVLAGTLFPVLSEAVRGSKI
                     SVGPPFFNRVNIPIAMFLLFLTGVGPLLAWRKTSVESLRKNFGYPLMGGFIAAIIAVA
                     LGLREFYVTLCVLLSVFVTLTILAEFYRGARVISARTGSNIFSSVSQLTMRNTRRYGG
                     YVVHFGMVLVFIGISGTAFNQDKQMDMSAGSQMILGPYTLHLQNYDSTQETNYSAERA
                     TIDVEKDGRSVMMLYPTRRFYPSNQESGTMVAISSTLRQDLYVVYAGTNPDTNLPVIH
                     AYLNPLVKWIWLGGCIVVLGTILALVPNRQAVMVLSSATAGQPVSYPGGTQPAQILNT
                     PRSKLPSDHD"
     gene            complement(3556..4002)
                     /locus_tag="VG272_03280"
     CDS             complement(3556..4002)
                     /locus_tag="VG272_03280"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HWF12732.1"
                     /translation="MKTKAKFGIGVAIILVTLSCLAYVGAEQSKTYYHTISEIPTLNG
                     SALHQRLRVGGDVKNGSIQHYSGRVDFVLVEQGKALPVSYVGTDPLPDTFKDGAQCLV
                     EGKVQPDGRFVAETVQAKCASKYEAAPGGQSQPGSAGDNSYPNGKS"
     gene            complement(4074..4673)
                     /locus_tag="VG272_03285"
     CDS             complement(4074..4673)
                     /locus_tag="VG272_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12733.1"
                     /translation="MEKSTNHLPCDGCGLPVTPEHIAERVHRLEQSTQFRPIHMGILF
                     VALAPPSHTADDFYAPARSREFSDLFLEALEIFPAEKPALEEDVRANDLARLAEFQRR
                     GHYLTYLSECPLLDDKETAEQIIQRLGPILLRRIKFNYRPRQIAPLGQELFPLIDLLN
                     GQGIGPVLTTADGQALPLPGTGSQEWMQLFRRSVTAGAT"
     gene            4733..5008
                     /locus_tag="VG272_03290"
     CDS             4733..5008
                     /locus_tag="VG272_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12734.1"
                     /translation="MHQFELWMHENCPPEKKGGRYKSHGDPVSLTPFRSPAQATALTL
                     EMEWRAVIGDGAPSITQGKSPQSPGVEPGQAIEQTQKSSSMKQTLVG"
     gene            complement(5121..5480)
                     /locus_tag="VG272_03295"
     CDS             complement(5121..5480)
                     /locus_tag="VG272_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12735.1"
                     /translation="MSKNTGTQVSEPAAASNGTTQVFRPYERLVTIKILGKSFDVPEK
                     NTILRAFQYISPETIPYGRFCWNQDCQYCRVVGQLPDEDRPHELLSCKFMVSAGMEIT
                     EISPELKYCLQNKLGKK"
     gene            complement(5776..6732)
                     /locus_tag="VG272_03300"
     CDS             complement(5776..6732)
                     /locus_tag="VG272_03300"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HWF12736.1"
                     /translation="MAQDSRKTDIEKLERELTDLERLSKPDAPSAELYRLRSEVAQLR
                     RDFYEHMGAWQRTLLARHPQRPYMMDYVRLLFEDFQELHGDRAYGDDPAMVTGFAKFN
                     GRPIVLVGHQKGRDTKQRVAQNFGQAKPEGYRKALRVMQLAAKFGRPIFTFIDTPGAY
                     PGIDAEERGQAEAIARNLREMARLPVPIVVTITGEGGSGGALAIAVGDRVNMLENSVY
                     SVISPEGCASILWRDSTKAEVAAEALKITAPHLKEFGIIDEIVPEPEGGAHTDVDASA
                     SFLGKVLDRALRDLTKLPIPELLDQRYEKFRRMGQFFETVRN"
     gene            complement(6854..10372)
                     /gene="dnaE"
                     /locus_tag="VG272_03305"
     CDS             complement(6854..10372)
                     /gene="dnaE"
                     /locus_tag="VG272_03305"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWF12737.1"
                     /translation="MENPRFVHLHLHTDYSLLDGACDLEEVVDEAARRKMPAVAVTDH
                     GNLFAAESFYQTAKTRGVKPIIGCEVYVAKGSRHDRGENSQRDAANGHASPAAEPGQR
                     GNNHLVLLCENAEGYKNLVKLVSAGFLEGFYYKPRIDYNLLAQHSKGLIALSACLRGA
                     VTENLVEDRPDQAREAAYKLRDIFGKGNFFLEIQDQGLEIEKRVNPKITELSRETGIP
                     LVATNDCHYLKHSDAHAQEVLLCIQTGKTMNDTHRMKFPTDQFFFKTAEEMAQVFKEI
                     PEALERTVTIAERCNVKIDAVQNSFPDFQVPEGHTVDTYFEAVSREGFVSRVPILEAR
                     AKAGMLRKTLGEYETRLSNEIHMIQKMRYAGYFLIVWDFIRHARAQGIPVGPGRGSAA
                     GSLVSYALQITDVDPLQYDLLFERFLNPERVSMPDIDIDFCMRRRGEVIEYVTGKYGR
                     SNVAQIITFGTMAAKAAIKDVGRAMDLPYGEVDRIAKLVPAQLNITLDEALKQAPQLK
                     ALIDGDARYTDLMEVAKRLEGLARHASTHAAGVVISPEPLTNLVPLYKSNRDEITTQY
                     DMKGLEHIGLLKMDFLGLTTLTVLDDTVHMIAQNRNQQIDLSTLPLDDTDTYKLFARG
                     ETTAIFQFESHGMRDILRRYQPTRLEDLTALNALYRPGPIQGGMIDDFINRKHGKKKV
                     TYDLPELKEILEETYGVILYQEQVMQIANRLPGFSLGEADILRRAMGKKNHAEMAAQK
                     QKFVAGSVARKFSEKKAEKIFDLMAEFAGYGFNKSHSCAYALLAYQTAYLKTHYPVEF
                     MAALLTSETGNTEKVVKYINESRGMGITVLPPDVNSSDLDFTPVAEAIRFGLRAIKNV
                     GENTVKGILEARAELGKFTSIYQFCDHIDTKLLNKRVLESLIKSGAMDSLGARRSQMF
                     AAIDRAIEAAQRRQREKNSAQHALFGGVSAVAEPVDEPLPNIEEWPEHEILASEFATV
                     GFYISGHPLSKFASRLKELNALELATVEGRRNGEEITVAGIVIAMRSMRSRKGDRWGI
                     LTLQDMTGVLEVLAFPESFGRLEPLFKSNAPLVLKGRVNVEEAGTRLAVTEARKLEDM
                     AQRLPAVMRVKVNLGTVDAGVLDELKELFVNRPGPCAVAFEIVSPEGAVATLRVDQRV
                     KADQDLVNEVCRLCGDSSVQLQARTS"
BASE COUNT         2520 a         3016 c         2838 g         2445 t
ORIGIN      
        1 tagcgggcgc agtcgaatta tattcgtgct gaatttcatt cgcaaaatac atcctcttta
       61 attctagtca tttttaaatc aaaagttttt gctgctcttt tcacaatcaa ttctacgagt
      121 ctgatatttt ctctgccaaa atccgcaatc tgaagaaagt gcggagcaac ccaagatttt
      181 gtcattccga gcgaagcgag gaatctctct tcgatttaag tacaagaaaa caaagaaaag
      241 gagagattcc tcgcttcgct cggaatgaca gaataaaaac ttttcccgca gactgtgaag
      301 cctggaacca tataaatcgg gtgatttatt gcctgggatt cgcggcattg accggatcgc
      361 gtcgcggaat cggcgcttga tttccagtca cgcgttcaat ttccagaagc acttgcgccg
      421 cctcggtttc gagcgcatcc cgcatctgtt cgtaatcctg ttccgcgtat ttgcccgcgc
      481 gattttcaaa tttcaggtcg cgcaaattat cgtaaatcga gtcacggcgc tccatcaatt
      541 gatcgagctg cgagcgatgc ggcgcggaat ctcctggatc aggctcaatc caaaacacaa
      601 aaatgaccgc ggccaatgtc agcgcgacgc acgcggaaag catgatcgtg tccggaaatt
      661 gagtcgcaat gagcgcagcc gtcataattt ttaatctcta ttcctcggtc tcgcggtccg
      721 cttgctgccg ggcgcgttcc agttgaacgt cgcgaagttc agacgccgag gtcggagcaa
      781 ctggcggcgc gctggacgct gttgcaaggc gcgcgacatc acgctgccgc cacaatcgaa
      841 tcaacgtgat tacaattcca agtccgatgg caaaggccac accggggatg taccacgcaa
      901 tgctgttgaa tccttttgtg ggcggcgcgc ttaaaacttt ttcgccgtat tcctgcacaa
      961 aatcctggac gatcaggtcg tccgattcgc ccttggcgac gtgggcgtcc agctctttca
     1021 gcatcgggcc cgacgacggg caattaatgt gattgcactg cgtaagaatc tgcccgcacc
     1081 cgcacatgca catcagcttc gtacctaaag atttggcgcg atcggttttt tgcgccgata
     1141 cgtgtgtagg cacaagcgcc gtggcgagag ccacaaacgc ggcgcagata aacgcgaagt
     1201 ggcggcggtt agtcatgatc gcttggcagc tttgatcgcg gtgtattcaa aatttgcgcg
     1261 ggttgcgtgc ctcccggata ggaaacaggc tggcccgccg tggcggatga caaaaccatt
     1321 acggcttgtc tgttcggcac gagagcaaga attgttccca ggaccacgat gcaaccgccc
     1381 aaccaaatcc atttcaccag cggattcaag taggcgtgaa tcacgggaag attcgtgtcc
     1441 ggattcgttc cggcatacac aacgtaaagg tcttggcgaa gcgtcgaaga aatcgccacc
     1501 atggtaccgg attcctggtt ggaaggataa aaacgccgcg tgggatacaa catcatcacg
     1561 gagcggccgt ccttctccac gtcaatcgtg gcgcgttcgg cgctgtaatt cgtttcctgc
     1621 gtggaatcgt aattctgcag gtgaagcgtg tagggcccaa ggatcatctg agagccagcg
     1681 gacatatcca tctgcttatc ttgattgaat gccgtgccgg agatgccgat aaacacaagc
     1741 accatgccaa agtggaccac gtagccgccg taacgccgcg tgttgcgcat cgtgagctgc
     1801 gatacggaag aaaaaatatt ggagccggtg cgggcggaaa tcacgcgcgc gccgcggtaa
     1861 aactccgcga ggatcgtcag tgttacaaac acggagagta gcacgcacag cgtaacgtaa
     1921 aattcacgca agcccagcgc gaccgcaatg atcgcagcga tgaagccgcc catcagtgga
     1981 tagccgaaat tctttcgcag gctttcgacc gaagtttttc tccaggcaag cagcggacct
     2041 acgcccgtca ggaacaggag aaacatcgcg atgggaatat tcacgcgatt aaaaaatgga
     2101 ggaccgacgc tgatcttcga tccgcgcact gcttccgaca gcaccgggaa caaggtgccg
     2161 gccagcaccg cgacacaggc aaccagcagg atcaggttat taaacagaaa gctggattcg
     2221 cgcgatagca tggaatcgag ttgattgtcg ctgcgcaaat aatcgcgatc cttaaaatac
     2281 gccgcgaggc acacgatgac gatcgtcagc aggaaacccc agaaccagtg accaatattc
     2341 gattccgcaa acgcgtgaac ggaatttacc gcgccgctcc gcgtcagcga cgtccccaaa
     2401 atgcataaca tgaacgtgag aaagaccagc caaacattcc aaacgcgcat catgccgcgt
     2461 ttttcctgca tgatcaccga atgtaaaaac gccgtgcccg taagccacgg catcagcgat
     2521 gcgttttcga ccgggtccca tccccagtaa ccgccccagc ccagcaccgc ataggcccag
     2581 tgcgctccga gcagaatgcc cgcggactga aacgcccacg caatcatggt ccagcggcgc
     2641 gtcaggtgaa tccacttttc tcccggatat cgccccagca gcgcggcgag cgcgaaggca
     2701 aatggaatag tgaagcccgt ataaccggaa tacaaagtgg gcggatgtat cgccatttcc
     2761 ggatattgca agagcgggtt aaggccgctt ccatcggcgc gcgtcgccag atgcagaatc
     2821 cctcccgcac cgtgacctac gaaaacgccg taggggctcg cgacaaaatt attcatggtc
     2881 agaaagaaaa tctgaacgcc ggccagtacg acgcccacat agggcatcag ctcaggatgc
     2941 tttccgcgat atgtaaacaa tgcggaaaac acgtaaatcg aaagcaagaa gctccagaag
     3001 agtaacgagc cttcctgacc ggaccacaat gcggaaaatt tgtaataagg agaaagagtg
     3061 cggttactgt gggctgctac ataagccaga gaataattat tggtgaaaaa cagataccac
     3121 aggctgcaag ttccaatcca gatgagtacg cagacggcga agcccgcatt gcgcgcggat
     3181 ttaattaaca gaggccgccg ggtaatgatc gcggcgatac cgccaaaaat tgcatacacc
     3241 gcgaaagcga aggccagaag taatgagtaa gcgccaaaca gatccaatgt gagtcctcga
     3301 ttccgggctg atcgccggca tcgtgcggga tcatttccga taagaatcca ctaccggtgc
     3361 acgtaaatta tctgcgtatt aaatatcaag ccaacttttg caacccatct tactaaatgt
     3421 ggcttgtggg tctcggcttc agccgcaaac tcaatctacg aaggaaaacc gggctttcgc
     3481 ccctggacgt caatcgtgtc ctcgtccaag tcgtttatga aatgagttta tgctaaatac
     3541 acgttgagta tcgacctagc tcttgccgtt cgggtatgaa ttatcgcctg cgctacccgg
     3601 ttgagactgg ccccccggcg cagcctcata cttagacgca catttagcct gaacggtctc
     3661 cgcgacaaat cgcccatcgg gctgcacttt gccctcgacc aagcactgcg caccatcctt
     3721 aaaagtatcc ggcagcgggt ccgtacctac gtagctaacg ggcaaagctt ttccctgctc
     3781 aaccagaacg aaatccacgc ggcctgaata atgctgaatg gaaccatttt tcacatcgcc
     3841 gccgacgcgc aagcgctggt gcagcgccga cccattcagc gtcgggattt ccgaaatcgt
     3901 gtggtagtaa gttttgctct gctccgcgcc tacataggcc aggcaagaaa gcgttacgag
     3961 aataatcgca acccctatgc caaatttcgc tttggttttc acgaattccc cctgctcaaa
     4021 agactgctta gtaagtgtaa gtcaaattct atcatacccg tgggttgatt cgcttaagta
     4081 gccccagcgg ttaccgaccg tcggaacagt tgcatccatt cctgcgagcc tgtacccgga
     4141 agtggcaggg cctgaccgtc ggccgttgtc aagaccggcc caatgccttg accatttagc
     4201 agatcgatca aagggaaaag ttcttgtccc aatggcgcaa tctgccttgg ccggtaatta
     4261 aatttgattc gccgaagcag tatcggaccg aggcgctgga tgatctgctc agcagtttct
     4321 ttgtcatcga gtagcgggca ttccgataga taagtcaggt agtggcctct gcgttgaaat
     4381 tcggcgagtc tggccagatc gtttgcgcga acgtcttctt ccagcgcggg tttttctgcc
     4441 ggaaaaattt cgagcgcttc caagaacaga tcggagaatt ctctcgatct cgcgggcgcg
     4501 taaaaatcgt cagcggtgtg ggacggcggc gctaaagcta caaatagaat gcccatgtga
     4561 ataggccgaa attgtgtcga ctgttccagg cgatgcacgc gttcggcaat atgttcaggc
     4621 gtaactggca ggccgcaccc gtcgcaaggc agatgatttg tggatttttc catacaggtg
     4681 gaacatccta cagcaaatgg cgggagaggt acaggaatgg cagaccatgt gagtgcatca
     4741 atttgagttg tggatgcacg aaaattgccc gcccgaaaag aagggcgggc gctacaaaag
     4801 ccacggcgat ccggtttcgc ttacgccttt tcgttccccc gcgcaagcca ccgcgctgac
     4861 cctcgaaatg gaatggcgcg ccgtaatcgg ggacggcgcg ccctccatta ctcaaggcaa
     4921 aagccctcag tctccagggg tggaaccggg ccaagccatc gagcaaactc aaaaatcgag
     4981 ttcaatgaag caaacacttg tgggttgaaa aagttgcgtt tgtaaggaaa gttgcgctgg
     5041 actaacttgc ggagattgtc ttgcttgggg acactttcac acgacaactc agggaacgcc
     5101 aggtgcgctc cccgttgaaa ctacttttta cctagcttgt tttgcaggca atacttcagt
     5161 tcagggctga tctcggtaat ttccatgccc gcgcttacca tgaatttgca gctgaggagt
     5221 tcgtggggac gatcttcgtc gggaagttgg ccgacgacgc ggcaatattg gcagtcctga
     5281 ttccagcaaa aacggccgta agggatagtt tccgggctga tgtattgaaa ggcccgaagg
     5341 atcgtgtttt tctccgggac atcaaaagat ttcccaagaa tcttgattgt aaccagccgt
     5401 tcatacgggc gaaacacctg cgtcgtccca ttactcgccg ctgccggttc cgatacctgc
     5461 gttcctgtat tctttgacat gaagggcctg tctccgtctc atcagagtaa ggcgggaagc
     5521 ggtggcgcaa caacggggat gctgcaaagt ggtgcgaatc cggcaatggt actttggacg
     5581 cgcaattcga cgcttgtggc gcgaaaacta gggatgcggc acgcaatatc ctcgcccgta
     5641 aaattccgtg tgataactag tctgtgttct gtgtagcgcc cgccttcagg cgggcattct
     5701 tgctcgcact ggaaatgccc gcctgaaggc gggcgctaca catgcatccc acacggatat
     5761 gtaaaagcaa aaagtttagt tacggacagt ctcgaaaaac tggcccatgc ggcgaaattt
     5821 ttcgtaacgc tgatcgagaa gttcagggat cggcagcttc gtgagatcgc gaagtgcgcg
     5881 gtcaagaacc ttccctaaaa atgaagcgga agcgtccacg tcggtgtgcg caccgccttc
     5941 aggttccggc acaatttcgt caatgattcc gaattctttt aggtgcggcg ctgtaatctt
     6001 cagcgcctcg gcggcaactt ccgctttcgt cgagtcgcgc cacaggatcg atgcgcaacc
     6061 ttccggagaa atcacggagt acacggaatt ttccagcatg ttcacgcgat cgcccactgc
     6121 gatagccagc gcgccgcccg aaccgccctc tcccgtgatg gtcacgacaa tcggcaccgg
     6181 tagccgcgcc atttcacgta agtttcgcgc gatggcttcc gcctggccgc gctcttccgc
     6241 gtcaatgccc ggataggcgc ccggcgtgtc aataaaagta aatatgggcc gaccgaattt
     6301 tgcggcaagt tgcatcacgc gcagcgcctt gcgataaccc tctggtttgg cttggccgaa
     6361 attctgggcg acgcgctgct tcgtgtcacg gcccttttgg tgcccaacga gtacgatggg
     6421 acgtccgtta aacttggcga acccagtcac catggcaggg tcatccccat acgcacggtc
     6481 accatgcagc tcctggaaat cttcaaacaa caggcgaacg taatccatca tgtagggacg
     6541 ctgtgggtgg cgagccaaaa gagttcgttg ccatgcgccc atgtgctcgt agaaatcgcg
     6601 tcgtaattgc gcgacttcgc tacgcaaacg atacaattca gcggaagggg cgtctggctt
     6661 cgacagccgc tcaaggtctg ttagttccct ctccagtttt tcgatatcgg tttttcgaga
     6721 gtcttgggcc ataagttttt tcatgtcgca ctaaaaactc attccgataa gagcacatcc
     6781 gaccctatat gggcaatcgg ccggcagacc tgaaccaaaa tccccgcctc tgaagaggcg
     6841 gtctacaaaa aagctatgat gttcgcgctt gcagttgcac cgaactatcc ccgcacaaac
     6901 ggcacacttc attcaccaag tcctggtccg cctttacgcg ctgatcgacg cgaagcgtcg
     6961 ctaccgctcc ttccggagaa acaatttcaa aagcgaccgc gcacggaccc ggccggttaa
     7021 cgaataattc cttcagttca tccagcacgc cggcatcaac cgtcccaaga ttaactttta
     7081 cccgcatcac cgctggaaga cgctgcgcca tatcctcgag cttgcgcgct tccgttaccg
     7141 cgaggcgtgt ccctgcttct tcaacgttta cgcggccttt cagcaccaaa ggcgcattgc
     7201 tcttaaacaa tggttcgagc cgcccaaacg attcaggaaa tgcaagaact tcgaggactc
     7261 cggtcatatc ctgcagcgta agaattcccc agcgatcgcc tttgcgcgac cgcatactgc
     7321 gcatcgcgat cacaatcccc gcgacggtaa tttcttcgcc gtttctgcgt ccctcaaccg
     7381 tggccagctc cagagcgttc aactctttga gcctcgaggc aaatttcgag agcggatgtc
     7441 cagaaatata aaagccgacc gtggcgaatt ccgaagcgag aatttcgtgc tccggccatt
     7501 cctctatatt cggcaatggc tcatcaaccg gttcggcgac tgccgaaacg ccgccaaaca
     7561 aagcatgttg agcgctgttt ttttcgcgct ggcgccgctg cgccgcttcg atcgcgcggt
     7621 caatcgccgc gaacatctgc gaccggcgcg ctccaagcga atccatcgcg cctgatttga
     7681 tcaagctttc gaggacgcgc ttgttcagaa gcttcgtatc gatgtgatcg cagaattgat
     7741 agatcgaagt gaactttccg agttccgcac gcgcttccag aattccttta acggtatttt
     7801 ctcccacgtt tttaatggcg cgcagtccga agcgaatcgc ttcggccacg ggagtgaaat
     7861 ccagatcgct tgaattcaca tcgggtggca gtaccgtgat tcccatgcca cgcgattcgt
     7921 taatgtattt tacgactttt tccgtgttgc ctgtttccga cgtgagcagc gcggccatga
     7981 actcgacggg atagtgcgtc ttcaaatacg cggtttggta ggccaacaat gcataggcgc
     8041 aggaatgcga tttattgaac ccgtagcccg cgaattccgc catcagatcg aatatcttct
     8101 cggctttttt ctcggagaat tttcgtgcga cggagcccgc cacaaatttc tgtttttgcg
     8161 cggccatttc cgcatgattt tttttgccca tggcgcgccg cagaatgtcc gcttcgccga
     8221 gtgaaaatcc gggcaggcgg ttggcaatct gcatcacttg ctcctgatac agaattacgc
     8281 cgtaagtctc ttctagaatt tctttcaatt cgggcaaatc gtacgtcact tttttcttgc
     8341 cgtgcttgcg gttgatgaaa tcgtcgatca tgccgccctg aatggggccc gggcggtaca
     8401 acgcgttgag cgcagtcagg tcttcgaggc gtgtcggctg gtagcggcgc agaatgtcgc
     8461 gcatgccgtg cgattcaaac tggaaaatcg cggtggtttc tccacgcgcg aaaagcttgt
     8521 aggtgtctgt atcatcgagt ggcaacgtgc tcagatcgat ctgctgattg cgattctgcg
     8581 caatcatgtg cacggtgtcg tccagcacgg tgagcgtcgt caggccgagg aaatccattt
     8641 tgagcaggcc gatatgctct aggcccttca tgtcatattg cgtcgtgatt tcatccctgt
     8701 tggacttgta cagcgggacg aggttcgtga gcggctcggg cgaaatcaca acgccggcgg
     8761 cgtgcgtgga agcatgccgc gcgaggccct cgaggcgttt cgcaacttcc atcaggtcgg
     8821 tgtagcgagc atcgccatcg atcagagcct ttaattgcgg tgcctgtttc aacgcctcat
     8881 ccagcgtgat gttgagttgg gcgggaacga gcttcgcgat gcgatccact tcgccgtagg
     8941 gtaaatccat ggcgcgaccc acgtccttga tcgccgcttt tgccgccatc gtcccgaagg
     9001 tgataatctg cgccacgttc gatcgcccat atttcccggt gacgtattcg atcacttcgc
     9061 cgcgccgccg catgcagaag tcgatatcga tatcgggcat ggacacgcgc tcgggattga
     9121 ggaaacgctc gaatagtaga tcgtattgca agggatcgac gtccgtgatt tgcagagcgt
     9181 aactcaccag gctgcccgcg gcggaaccgc gtcccggccc caccggaatt ccctgcgcgc
     9241 gcgcgtgacg aataaaatcc cacacgatca ggaaatatcc ggcgtagcgc atcttctgaa
     9301 tcatgtgaat ttcgttggac aagcgcgttt catactcgcc caaagttttc cgcagcattc
     9361 cggccttcgc gcgcgcttcc agaatcggaa cgcgtgaaac aaatccttcg cgcgaaacgg
     9421 cctcgaaata cgtgtcgact gtatggcctt cgggaacctg gaaatccggg aaagaattct
     9481 gcaccgcgtc gatttttacg ttgcagcgtt ctgcgattgt caccgtgcgt tccagcgctt
     9541 cgggaatttc cttaaagacc tgagccattt cttcggccgt cttgaagaaa aactggtcgg
     9601 tgggaaactt catgcgatgc gtgtcgttca tcgtcttgcc cgtctgaatg cagagcaaaa
     9661 cttcctgcgc atgcgcatcg gaatgtttca aatagtggca atcatttgtg gcgacgagcg
     9721 gaatgcccgt ctcgcgcgac agttccgtga ttttcggatt gacgcgcttc tcaatttcca
     9781 gtccctggtc ctgaatttcc aggaagaaat ttccttttcc aaaaatgtcg cgcagcttgt
     9841 aagccgcttc gcgcgcttgg tccggccggt cttccaccag attttcggtg acggctccgc
     9901 gcaggcacgc ggacagcgcg atgagtccct tgctgtgctg cgccaataaa ttgtaatcga
     9961 ttctcggctt gtaataaaaa ccttcgagga atcccgcgga gacgagcttt acgagatttt
    10021 tgtagccttc ggcgttttcg cacagcaaca caaggtgatt gttgccgcgc tgccccggct
    10081 cggcggccgg actcgcgtgc ccgttcgcgg catcgcgctg cgagttctct ccgcgatcat
    10141 gccggctgcc cttcgccacg tacacttcgc agccgatgat gggtttcacg ccgcgcgtct
    10201 tcgccgtctg gtaaaacgat tccgccgcga aaagatttcc atggtcggta acggccaccg
    10261 caggcatctt gcgccgcgcg gcctcatcca caacctcttc gaggtcgcag gccccatcga
    10321 gcaaactata atccgtatgt aaatgcaggt gaacgaatct gggattttcc atgctgaaga
    10381 ctattctaat ctcacgggcc aaattttgct acgccgcaga caagctttcc tcagcgctcg
    10441 aataaagttt ctcgaggaaa ttgcggaaga aaacaagtcg ccctctccgt cccgaagaac
    10501 acgggacgaa gaggcctcta cgaaaaccga agtgcttgcc tttgctttac atagaggccg
    10561 cccgtccttc gggcgggcag cttttctgag agtttaggta aattcacgcg cgtttccgga
    10621 ttttttgcca ggcctgatcg taaaccagca cccttcacaa atcgtgaagg atgcgacacc
    10681 caaagagtca aaccttgccc aaactctgac ccacccgccc aagttgatgg tggccccccg
    10741 tgatctgtcc aatatgtaat tcgtcttcgt gtttcttagg aatttaagga gcaacgcgta
    10801 tgccgttgtc ctcagcctg
//
LOCUS       DASZMB010000071         5404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3375819, whole genome shotgun sequence.
ACCESSION   DASZMB010000071 DASZMB010000000
VERSION     DASZMB010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3375819"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(390..764)
                     /locus_tag="VG272_03310"
     CDS             complement(390..764)
                     /locus_tag="VG272_03310"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWF12738.1"
                     /translation="MNLRRMLGISVAIVLAAAVMVSAQGNPKGKESQLRTVRGTVVDD
                     TEAPSDSAVVYLKNSRTQDIATHLSSTDGTFRFSGLDLNVDYEIHAEKDGWTSSSRSI
                     SNFDTRKEFVLTLKLDHKKGGK"
     gene            complement(761..1159)
                     /locus_tag="VG272_03315"
     CDS             complement(761..1159)
                     /locus_tag="VG272_03315"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWF12739.1"
                     /translation="MALALVGFVLLACTVPAHEWAGVPQSPSSPHAHDFVVFTTVFTN
                     QGFALPGARARMRRPDDKKFRWEAISDRRGELAIRVPQNSGYELTVEARGYKTQTQKV
                     DATEGNRADLTIRMEALSGPHSGSGTGGKS"
     gene            complement(1221..1457)
                     /locus_tag="VG272_03320"
     CDS             complement(1221..1457)
                     /locus_tag="VG272_03320"
                     /inference="COORDINATES: protein motif:HMM:NF044773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12740.1"
                     /translation="MRCPKCSTEIDVDEDEVEEGEILACPECETEMEVVQIHPVKVNA
                     ISEDDDEDEDEESEDGDDLEDDDEEDEEEEEEDV"
     gene            1581..2084
                     /locus_tag="VG272_03325"
     CDS             1581..2084
                     /locus_tag="VG272_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWF12741.1"
                     /translation="MALRREYPELPMVGVGGVVVHEGRALLIRRGSAPLKGEWSIPGG
                     LLEVGETLEQGVMRELAEETGLDVQVIELIEVFERIFPAPPNADGTPGDCSRPQYHFV
                     ILDYLCETRGGTIAAASDALEFAWAREEDLVKYDVTVAVKRVLRKAFKRAHERAASFG
                     DPASPSA"
     gene            complement(2112..2795)
                     /locus_tag="VG272_03330"
     CDS             complement(2112..2795)
                     /locus_tag="VG272_03330"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HWF12742.1"
                     /translation="MRKTDLFFDVFGTARRTMVETQIRKRGVASPRVLEAMATVPRHE
                     FVSAEFQNDAYADKPLPIGEGQTISQPYMVAAMTEALELTGSERVLEIGTGSGYQAAV
                     LSSLASQVISVENHPSLALSAQERLWRLGYANVHVHCGDGSLGLPDAAPHDAILVTAG
                     APEIPLVFAHQLREGGRIVIPVGDRENQELVSAQKINGELKLRVLFGCRFVPLLGRFG
                     WARDERKFQ"
     gene            3119..3403
                     /locus_tag="VG272_03335"
     CDS             3119..3403
                     /locus_tag="VG272_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12743.1"
                     /translation="MIREFLPQLRNKKAQRFAARVLRGIAFLVLALFVAQGARAQKIT
                     MEFDQSIDFSRYKTFAIRDGQLNSRNPALGGELVKKQIETDIQNDLTAKG"
     gene            3406..3693
                     /locus_tag="VG272_03340"
     CDS             3406..3693
                     /locus_tag="VG272_03340"
                     /inference="COORDINATES: protein motif:HMM:NF024978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HWF12744.1"
                     /translation="MAKNGPADLNVRYTFGAARKTEVEAYPAGWYGRGTRYVRVPYAE
                     GTLVIDLRDPTTKSLVWRAIAAEEKSDASKIQGKINDMVKKSIDKYPPKKK"
     gene            complement(3846..5135)
                     /locus_tag="VG272_03345"
     CDS             complement(3846..5135)
                     /locus_tag="VG272_03345"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF004126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HWF12745.1"
                     /translation="MSRDTLSITDNRTGKSYEVPIKDETIRATDLRQIKVKGDDFGMM
                     SYDPAFNNTALCQSKITFIDGDKGILRYRGYSIEELAEKSTYLETAYLILHGELPTRT
                     QLEDWSYHITHHTFIHESVKKFLDGFHYDAHPMGMMISAIAALSTFYPEAKDIFNKDS
                     RKKQTYRLIGKMPTLAAFSYRHSLGMPFAYPDNELSYPGNFLNMLFKTSELKYRPNPT
                     LERALDVLFILHADHEQNCSANAMRCVGSSHVDPFSATAAATAALYGPLHGGANEEVL
                     RMLLEIGSLANVPAFIKRVKAGETKLMGFGHRVYKNYDPRARIIKHVADGVFEIMGRN
                     PLLNIALECERIALEDDYFVSRKLYPNVDFYTGLIYQSMGFPVTMFPVLFAIPRTSGW
                     IAQWEEMLLDPEQKIARPRQVYTGYDLRPYASLDQRK"
BASE COUNT         1202 a         1462 c         1518 g         1222 t
ORIGIN      
        1 aggtcaaccg attgtggctt gccattcaca aaaaattgaa ctgccatggg agtcctccgc
       61 gggaaattaa ttcgttaccg gcgtctaaat tgttgagatt gaggcgcgcc agtttgggtg
      121 cgagccatgt tactgcaatt ccgttggccc ggtcacggaa aaaatcagcc ggtttaaagg
      181 cttgcaaata aaacgtgcag ttcgtcagcc gcgggttgac tttgctgtat cccgcctctt
      241 caggggctgg ggcttagctg gaccgcgaag cgcgcttcac ccggcgtcta aattcgtcta
      301 aattccctcc tctgaagagg agggctacag cagcagggcc aactcggctt tgactggctt
      361 cacaggcttc gatggaagaa aagacacggc tatttcccgc cttttttatg atcgagcttc
      421 agcgtaagga cgaattcttt gcgagtgtca aaattggaga tcgatcgcga cgatgaggtc
      481 cagccgtctt tttcggcatg aatctcgtaa tcgacattga ggtcgagtcc gctgaaccgg
      541 aacgtgccgt cagtgctcga gagatgcgtg gctatatcct gcgtgcggga atttttgagg
      601 taaacgacgg ctgagtcaga cggcgcttcg gtatcgtcca caaccgtgcc acgcacggtt
      661 ctcagctgcg actcttttcc tttcggattt ccctgtgcag aaaccattac ggctgcggcc
      721 aatacaatcg caacggatat tccgagcatt ctacgcaggt tcatgatttt cctccggtcc
      781 ctgatccgga atgcggcccg gacagcgcct ccattcgtat cgtcaggtct gctcgatttc
      841 cttccgtcgc atccactttc tgagtttgcg tcttatatcc gcgcgcttcc accgtcagtt
      901 cgtaaccgga attctgcggg acgcggatcg ctagttctcc ccgacggtcg gaaatggctt
      961 cccatcggaa cttcttatca tccggccggc gcatgcgcgc gcgggctccc ggcaacgcga
     1021 atccctggtt ggtaaatacc gtggtgaaaa caacaaaatc gtgggcgtgc ggagaactcg
     1081 gactctgcgg aacgccggcc cactcatgcg caggcactgt gcacgccaga agcacaaatc
     1141 ccacaagcgc aagtgccatc cacactaaag taagacggcg aatggctgtg atggggtgcc
     1201 aaaagacgcg aacaggtctt tcagacgtct tcttcttcct cttcttcgtc ttcttcgtcg
     1261 tcgtcctcaa gatcgtcccc gtcctcggac tcctcgtcct cgtcctcatc atcgtcttcc
     1321 gaaatagcat ttactttgac ggggtggatc tgcacgactt ccatttcggt ttcacattcg
     1381 gggcatgcca ggatttcgcc ctcctcgacc tcgtcttcat ccacatcaat ttcagttgag
     1441 catttaggac accgcaccac ggcgaaaacc tcctaggagt ttggggacag gctcactcca
     1501 agcccgccga ctgcaagaca cacctgctaa agtattatat aacgcgaagc ggcttcagat
     1561 ggaagcgggc aaaagcgacg atggcactcc gaagagaata cccggaactc ccgatggtgg
     1621 gagtaggcgg tgttgtggtt cacgagggcc gcgcgctgct cattcgacgc ggcagcgcgc
     1681 cgctcaaggg ggagtggtcc attcccggcg gtttgctgga agtgggggag accctcgagc
     1741 agggtgtcat gcgcgaactc gccgaggaga cggggctcga tgtgcaggtc attgaactca
     1801 tcgaagtttt tgagagaatt ttcccggcac ctcccaacgc cgatggcacg ccgggcgatt
     1861 gctcgcgccc gcaataccat ttcgtgattc tcgattacct ttgcgaaact cgcggaggga
     1921 cgattgccgc ggccagtgat gccctggaat ttgcctgggc gagggaagag gatttagtga
     1981 agtacgacgt aacggtagcc gtgaaacgcg tcttgagaaa agcatttaag cgggcgcacg
     2041 aacgagccgc gagttttgga gaccccgcga gcccaagcgc gtaggtgaga aaattcggcg
     2101 aggtagtgca cttattgaaa ttttctttcg tcccgcgccc agccaaatcg tcccagcagc
     2161 ggcacaaatc tgcagccgaa tagcacgcgc aatttgagtt caccgtttat cttttgagcg
     2221 gagaccagtt cctgattttc gcgatcacca acgggaatga cgatgcgccc gccttcccgc
     2281 aactgatgcg caaacacaag cggaatttct ggcgcccccg ccgtcacaag tatggcgtcg
     2341 tgcggcgccg cgtcgggcaa gcccagcgag ccgtctccac agtgcacgtg tacattcgcg
     2401 tagcccagcc gccaaagccg ttcttgtgca ctaagcgcaa gcgacgggtg attttcaacg
     2461 ctgatcacct ggcttgcaag cgaggaaaga accgccgcct ggtagccgga tccggtaccg
     2521 atttccagaa cgcgctcgga gcctgtgagt tccagggcct ccgtcatcgc cgcgaccatg
     2581 taaggttgtg aaatcgtctg gccttcgcca atcggcaaag gtttgtcggc ataggcgtcg
     2641 ttctgaaatt ctgcggaaac aaactcgtgg cgcggaacgg tagccatcgc ttccaggaca
     2701 cgcggtgacg caacgccgcg cttgcggatt tgcgtttcca ccatcgtgcg ccgcgccgtt
     2761 ccaaacacgt caaaaaataa atctgttttc cgcacaggcc attataaatg ctgcgcaggc
     2821 gagtggcaaa agcgggcgat attgtggccg tgtgttagtc aaatttgtcg cgaatctaaa
     2881 cccgcccggc caaattacgg ccgtcctctg cgaatgcggt ttcgtagtgg cctgtccgtc
     2941 ccgcgttttc gggatggaca ggccgcttga tgcgcgaagg aggcctttac tccgcgcaga
     3001 gaactgtatg tcgctgccgt ccgcacccag ataacggaaa ctccacacgc cttctccatc
     3061 gctgctgaat ttttacgtag ccttgataca ctacggcagg cttttcgaga ggtgaatcgt
     3121 gattcgagaa tttcttccgc agctccgaaa caaaaaagct cagagattcg cggcccgcgt
     3181 cctgcgcggc atcgcgttcc tagtcttagc gttgttcgtc gcgcaaggcg cgcgagcaca
     3241 aaaaatcacc atggagttcg atcagtccat cgacttcagc aggtacaaaa ccttcgccat
     3301 ccgagatggc cagttgaaca gccgcaatcc agcgttgggc ggcgaactgg tgaaaaaaca
     3361 gatcgagacc gacattcaaa acgatttaac cgcgaagggt tgacgatggc gaagaacggc
     3421 cccgcagatc tgaatgtgcg ctacacattc ggtgcggcgc gtaaaactga agtcgaggct
     3481 tatccggccg gctggtacgg acggggcacg cgctacgtgc gtgtgccgta tgccgaagga
     3541 accctggtga tcgatttgcg cgatcccaca acaaagtcgc tggtatggcg cgcgatcgcc
     3601 gcggaagaaa aaagcgacgc cagcaaaatt cagggaaaaa tcaacgacat ggtcaagaaa
     3661 tcgatcgaca agtacccgcc aaaaaagaaa tgagtagcga gagcggctac cggtaatgag
     3721 cctcatctcg tgcgagagtg accaggtcag atttagggaa cgcttttttg gctaaaacgc
     3781 ggcgcataag gggctgtgtt tcttgcccat tccaggatcg cttgcttgga cctaaagttt
     3841 gtcagttact tccgttggtc cagagacgcg taaggccgca aatcgtagcc cgtatatact
     3901 tgccgcggac gcgcgatttt ctgttcagga tcgagcagca tttcttccca ttgcgcgatc
     3961 catccgctgg tgcgcggtat ggcgaagagc acgggaaaca tggtcacggg gaagcccatc
     4021 gactggtaga tgaggccggt gtagaaatcg acgttgggat acagcttgcg tgagacgaag
     4081 taatcgtcct cgagggcgat gcgttcgcac tcgagagcga tattcagcag cgggttgcgg
     4141 cccataattt caaaaacgcc gtcggcgacg tgcttgataa ttctggcgcg cggatcgtaa
     4201 ttcttgtaaa cgcggtgccc gaagcccatc agcttggttt cgcccgcctt cacgcgcttg
     4261 ataaacgcag gcacgttcgc gagcgagcct atttccagca gcatgcgcag cacttcttcg
     4321 ttggctccgc cgtgcagcgg tccgtagaga gcggcggtcg cggcggcggt cgcggaaaat
     4381 ggatccacat gcgaactgcc cacgcagcgc atcgcgttcg ccgagcaatt ctgctcatga
     4441 tctgcatgca aaataaacag gacgtccagg gcgcgttcga gcgtcgggtt cggccggtat
     4501 ttcagttcgg aagtcttgaa cagcatgttc agaaaattgc cggggtacga aagctcgtta
     4561 tccggatatg cgaaaggcat gcccaggctg tgccgataag aaaacgcggc gagggtgggc
     4621 atctttccga tcaggcgata tgtctgcttc ttgcgtgagt ctttgttaaa aatgtctttc
     4681 gcctcgggat aaaaagtgga gagcgcggcg attgcggaaa tcatcatgcc catcggatgg
     4741 gcatcgtaat gaaagccgtc caaaaatttc ttcaccgatt cgtggatgaa ggtatggtgc
     4801 gtgatgtgat aggaccaatc ttccagctgc gtgcgcgtag gcagctcgcc gtgcagaatc
     4861 agatacgctg tttcgagata agtgctcttt tccgctagtt cttcgatgga gtatccgcgg
     4921 tagcgaagga ttcctttgtc gccgtcaatg aaagtaattt tgctctgaca cagggccgta
     4981 ttgttgaagg ccgggtcata gctcatcatc ccgaagtcat cgcccttcac cttgatctgg
     5041 cgtagatcgg tagcccgaat tgtttcgtcc ttgatgggaa cttcgtaaga ctttcccgtt
     5101 cggttatcag tgatggaaag agtatcccgg ctcatgcggg tggtcccttc tgcggctacc
     5161 tgcgtctaaa gtttagttgg attcgcggcg ctttcagagt ttccgaaaaa gcaccacagg
     5221 agaatttaca ctaagttgac gtttttcggg agtagtatcc cgccgaattg tcttagctcc
     5281 ataattctgt caccccttaa cggctacgtg aaaatgtcac cctatgagac aggggacatt
     5341 tgcattgagc cagaaagaat tgcaacgagt ggcagtgatt tcgtcttgcg tgaaggggaa
     5401 cctg
//
LOCUS       DASZMB010000072         6386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1989446, whole genome shotgun sequence.
ACCESSION   DASZMB010000072 DASZMB010000000
VERSION     DASZMB010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1989446"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VG272_03350"
     CDS             <1..567
                     /locus_tag="VG272_03350"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWF12746.1"
                     /translation="NSWNERYQSRERAAEDLDTAPTPLLLSTALKLSPGKALDLACGT
                     GRNSLRLAERGWEVTAVDAASIAIEILQTRAMERDLKIDAIIADLEKGEFEIKPSCWD
                     LIAICYYLQRNLFEPAKRGVVPGGLLISIVHITEPGEEESPYRLRPGQLEKYFAGWEI
                     LHHHEGKANDAAHRRAVAEIVARKPMQS"
     gene            complement(586..1647)
                     /locus_tag="VG272_03355"
     CDS             complement(586..1647)
                     /locus_tag="VG272_03355"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: protein motif:HMM:NF006048.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HWF12747.1"
                     /translation="MKTYKIAVIAGDGIGKEVVPEGLRVLEAAGKKFGINYSFKHFDW
                     SCETYAKTGRMMPDDGLAQLQPFDAIFLGAVGYPGVPDHISLWGLLIPIRRSFDQYVN
                     LRPVKMLKGVDSPLKSAKPGEIDFCVVRENVEGEYSNIGGRIYNDTPEEMAMQQTIFT
                     RRGVDRIMRFSFELARKRKSHVTSATKSNGIIHTMPYWDERFAAMSAQYPDIRVDQYH
                     IDILTAHFVRRPQMFDVVVGSNLFGDILSDLGPAVVGSIGIAPSANLNPEKKHPSMFE
                     PVHGSAPDIAGKNIANPIGQIWSAAMMLDHFGEPAAAAAIERAIEEVLTKPEFLTPDL
                     GGKASTQVVGKAIADTLSK"
     gene            complement(1691..2500)
                     /locus_tag="VG272_03360"
     CDS             complement(1691..2500)
                     /locus_tag="VG272_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HWF12748.1"
                     /translation="MSRLKIGSMVAVCALFCAAVVLHAARPAAQTQAPLANDPKLIED
                     LVYANRILFAQSVLDGFGHVSARSDKDPSHFLMSRSMAPGLVTANDIMEYDRNGEPVD
                     ARGRNSYVERYIHAAIYRVRPDVKAIVHSHSPDIIPYSVTGTKLLPLYHVSAFLRLGA
                     PIFDTHEEFGDTDMLIRDNKLGDALAKVLGNSGIALIRGHGFVAVADSIPIVVYRAIY
                     TQTNARVQGEAMKLGTIKVLTPGEAVKAQAIVEATVGRPWELWKTQVGKIE"
     gene            2717..3685
                     /locus_tag="VG272_03365"
     CDS             2717..3685
                     /locus_tag="VG272_03365"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF12749.1"
                     /translation="MFTRREIVKGAVIAGAAGLAWRPDVSIASASQPSTPVNFAIPEG
                     ACDCHVHTFDPQHFPYSPTRPYTPEPVSVGELQSLHKALHMSRVIVVQTTVYASDNAG
                     VLDAMKKLGPRARGVAVIDDKTPDSALDEMDRAGMRGIRLNLETAGETDPAAGRKRFQ
                     AAVERIKNRKWHIQIYARLSVIDGIKDLVVAAPMPVVFDHFGGTQAAQGIEQPGLNSL
                     LGLLKGGKAYVKISAPYRSSTIAPDYPDVAAIAKVMIAANPQRILWGSDWPHPGTPVP
                     GRSISEITPFFKIDDGHVLNLLPKWAPDAAMRKTILVDNPARLYGF"
     gene            complement(3779..4285)
                     /locus_tag="VG272_03370"
     CDS             complement(3779..4285)
                     /locus_tag="VG272_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12750.1"
                     /translation="MTKLFGKGQFKMIFKHKCLGILALLLAVPTLGSVSYAAQYQEGG
                     PGRGQGRGGPRGPMSPDDQLKRMTKDLKLTDDQQSKIKPILVDEQKKMEDLRNDSSGD
                     RQAMRVKMMQIRKDTNDLVRVQLDDKQKEKFDKQEQEREDRMRNRRGPGPGPGGPDGD
                     NPPPPPQN"
     gene            4468..4944
                     /locus_tag="VG272_03375"
     CDS             4468..4944
                     /locus_tag="VG272_03375"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWF12751.1"
                     /translation="MGSMISIRIATENDIDAIVHLINEAFLVEQFFIERDRTDPPTVR
                     ALMEKGMFLLAEEDSMLVGCVFSELRIEHGYFGMLSIDPSRQGIGVGRQLVKAVEKYF
                     TDAGCKFSDLKIVNVRTELHDMYQRWGYVDTGTDVYDDPTPTKTPVHFINMSKPLQ"
     gene            complement(4958..5860)
                     /locus_tag="VG272_03380"
     CDS             complement(4958..5860)
                     /locus_tag="VG272_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HWF12752.1"
                     /translation="MPYATEENLTELALKQWEACHSPRLRQIMQSLVKHLHGFARDVE
                     LKQNEWLMAVEWLAKTGKLSSDKRQEFILLSDVLGISMLVDAMNHRFPEGATPSTVIG
                     PFHIDDSPEMEMGANVAEGLAGEPAFLVGTVRDMNGKPIEDAKIDIWQADADGLYESQ
                     LGADEPVLRAIFHTGPDGKYAIRTIAPPGYSIPMDGTVGDLLRQTDISHFRPAHIHFL
                     ISAPGYETLITHLFKKGAKYIDSDVVFGVKDKLIVEFEKHPAGKTPTGENSAEAFLVV
                     NYDFTLSKSKVRSESEPALSKAAG"
     gene            6116..>6386
                     /locus_tag="VG272_03385"
     CDS             6116..>6386
                     /locus_tag="VG272_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWF12753.1"
                     /translation="MAILVTGGSGYIGSVTVECLLAKGEDVVVLDDLAHGHRAALPAN
                     IPFYQGKIGDEDLVARIAREHALESCIHFAAMIEVGESVLDPAKYF"
BASE COUNT         1458 a         1786 c         1630 g         1512 t
ORIGIN      
        1 aacagctgga atgaacgtta ccaatcgcgt gaacgcgctg ctgaagattt ggacaccgcg
       61 cccactccgc tattactatc cacagcgttg aaactatccc caggcaaagc actcgaccta
      121 gcctgcggca caggccgaaa ttccctccgg ctcgcggaac gtggatggga agtcacggct
      181 gtagacgccg cttccatagc catcgaaatt ctgcaaactc gcgcaatgga acgcgactta
      241 aaaattgatg cgattatcgc cgatcttgaa aagggagaat ttgaaattaa gccttcttgc
      301 tgggacttaa tcgcaatctg ttactacctc caacgaaatc tttttgagcc cgccaagcgc
      361 ggcgtagtcc ccggcggcct gctgatttcc atcgtccaca tcacagaacc tggcgaagaa
      421 gaaagcccgt accgcttgcg tcccggccaa ctggaaaaat attttgcagg ctgggaaatt
      481 cttcatcatc acgaaggaaa agcaaacgac gcggctcatc gccgcgccgt cgccgaaatt
      541 gtggctagaa aacctatgca atcttagttc aaaaacgaac gaacgctact tgctcagcgt
      601 gtcggcgatc gctttcccaa ccacctgcgt gctggctttc ccgccgaggt cgggtgtcag
      661 aaattcgggc ttggtcagga cctcttcgat ggcgcgctcg atggcggctg cagcggccgg
      721 ctctccaaaa tgatccagca tcatggctgc ggaccagatt tggccgatcg ggttggcgat
      781 attttttccg gcgatgtccg gcgcggaacc gtggactggc tcgaacatcg atggatgttt
      841 tttctccggg ttcaaattcg ccgacggcgc gatgccgatc gagccaacca cggctggccc
      901 gaggtcggaa agaatgtctc caaaaagatt tgagccgaca acaacgtcaa acatttgcgg
      961 acggcgcaca aaatgcgcgg tcagaatgtc gatgtgatat tgatcgacgc gaatgtccgg
     1021 atattgcgcg gacatcgcgg caaaacgttc atcccaatac ggcatggtat ggatgattcc
     1081 gtttgatttt gtggcggaag taacgtgcga cttgcgcttc cgcgccagct cgaaagagaa
     1141 gcgcataatc cgatcgacgc cgcggcgcgt gaagatggtc tgttgcatgg ccatttcttc
     1201 aggtgtgtca ttgtaaattc gtccgccgat gttggagtac tcgccttcca cgttttcgcg
     1261 caccacgcag aaatcgattt cacccggctt cgcgcttttc agcggcgaat cgacaccctt
     1321 caacattttt accggccgca ggtttacata ctggtcgaag ctgcggcgga tcgggatgag
     1381 caggccccaa agcgaaatgt gatccggcac gccgggatag cccacggcgc caagaaaaat
     1441 cgcgtcaaac ggttgcagct gcgcgaggcc gtcatcgggc atcatccttc ccgtcttcgc
     1501 gtaggtttcg cagctccagt caaaatgctt gaacgaataa ttgattccga atttcttgcc
     1561 cgccgcttcc agcacgcgca atccttcggg cacaacttcc ttgccaattc catctccggc
     1621 aattaccgca atcttgtaag ttttcatttt tatgattccc gctctccgtt ttttattttt
     1681 atataaacaa ttattcaatc ttcccgactt gcgtcttcca taattcccac ggccggccaa
     1741 ccgtggcttc cacgatggcc tgcgctttga ccgcctcgcc gggcgtcaaa actttaattg
     1801 tgcccagctt cattgcttca ccttgaacac gcgcgtttgt ctgcgtgtaa atcgcacggt
     1861 acacgacaat cggaattgag tccgcaaccg ccacgaatcc atggcctcga atgagagcga
     1921 tgccgctgtt gcccaatact ttggctaatg cgtcgcccag cttgttgtcg cgaattaaca
     1981 tgtccgtatc gccaaattcc tcatgcgtgt caaaaatcgg cgcgcccaac ctgagaaatg
     2041 cacttacatg gtaaagaggg agcagttttg tgccggtaac actgtaagga ataatatccg
     2101 gggaatggct gtgcacgatc gctttcacat ccggccgcac ccggtaaatt gccgcatgaa
     2161 tgtagcgttc gacgtaggaa ttgcggccgc gcgcatccac cggttcgcca ttgcggtcat
     2221 attccataat gtcgttcgcc gtgacgagtc ctggcgccat gcttcgtgac atcaggaaat
     2281 ggctcgggtc tttgtcgctg cgcgcgctta catgtccaaa tccgtccagc acggattgcg
     2341 caaacagaat gcgattcgcg tagacaaggt cttcaatcag tttcggatca ttcgcgagag
     2401 gcgcctgcgt ttgggcagcg gggcgtgcag cgtgaagaac gaccgcggcg caaaataacg
     2461 cgcagaccgc caccatggag ccgattttca gtctcgacat tttcgtcctc cagatcgcga
     2521 agcaatccgt attcgcggat aaaacatgtc tcggcgaact gtagatgtta tacggtttcg
     2581 tcgattgtac caagagtgcg ggcgattatt ttacgcattc cgatgtgatc gcacgaaatt
     2641 tttctgagcg ttccagcgat acaaccccgc aagcttcgcg atatactctc gcaggaaatt
     2701 atccggagga aaatgtatgt ttacgcggcg tgaaattgtg aagggcgcag ttattgcggg
     2761 ggcggccggc ctcgcgtgga ggccagacgt gagcattgcc agcgcttcgc agccatcaac
     2821 tcctgtaaat ttcgcaatcc ccgaaggcgc ctgtgattgc cacgtgcata cgttcgaccc
     2881 gcaacatttt ccgtattcgc ccacgcgccc ttatacgccg gaaccggttt cggtgggcga
     2941 gctgcaatcc cttcataaag cgctgcacat gagccgcgtg attgtagtgc agaccacggt
     3001 ctacgcttcg gacaacgcag gcgtgctcga tgccatgaaa aagctgggcc cgcgtgcgcg
     3061 cggcgtcgcg gtgattgacg ataaaactcc ggattccgcg ttggacgaaa tggatcgcgc
     3121 gggcatgcgc ggcattcgtc tcaatctgga aactgccgga gagacagatc ctgccgctgg
     3181 tcgcaaacgt tttcaggcag cggtggaaag aattaaaaat cgcaaatggc acattcaaat
     3241 ctatgcgcgg ttgtcggtga ttgacggaat taaggatctg gtggtggcag cgcccatgcc
     3301 tgtggtcttc gatcacttcg gcggaacgca agcagcgcag ggtattgaac agccgggcct
     3361 caattccctg ctcggcctgc taaagggcgg gaaagcctac gtgaaaatct ccgcgccgta
     3421 tcgcagctcg acgattgctc cggattatcc ggatgttgcc gcgattgcca aggtcatgat
     3481 tgccgccaat cctcagcgaa ttttatgggg cagtgactgg ccgcatccgg gaacccccgt
     3541 acccggccgc agcatttccg aaatcacgcc gttttttaag attgacgatg gtcacgtgct
     3601 gaatttgctg ccgaagtggg ctccagacgc cgcgatgcgc aaaacgatcc tggtggacaa
     3661 tcccgcgagg ctgtacggat tctgaactga atttagcagc gcgggcttag cctgcgggtt
     3721 tagagcttgc atgcattaaa ccccacaggc tgaagcctat gccacctaaa accaaagctt
     3781 aattctgggg cggcggcgga ggattatccc catctgggcc tccgggaccc ggtccaggac
     3841 ctctgcggtt ccgcatgcgg tcttcgcgct cctgctcctg cttgtcgaac ttttcctttt
     3901 gcttgtcatc cagttgcacg cgaaccagat cgtttgtatc cttgcgaatc tgcatcattt
     3961 tcacgcgcat ggcttgacgg tcgccgctgg aatcgtttcg caaatcctcc atcttctttt
     4021 gctcatcaac caggattggc ttgatcttcg attgctgatc gtccgttaat ttaagatcct
     4081 ttgtcattcg cttgagctgg tcgtcgggag acataggtcc cctcggcccg cctcgaccct
     4141 gtcctctccc gggcccacct tcctgatatt gcgccgcata agaaacactg cccagcgtgg
     4201 gcacagccaa cagcaatgcc aaaatcccaa gacacttatg tttaaaaatc atcttgaatt
     4261 gccccttacc gaatagctta gtcatccaaa tctcaatcag acaaatcctt tacccctgcc
     4321 ctacctgtat acccgtaaca agggggctcg gttccacaat tgcttcgcag attcatggta
     4381 attaagaaaa tttgggtgat gaagaagaac tacccaggtg ccatccgcgt agcgaaatga
     4441 aacaagtccc aggaattgat atgatttatg ggctcaatga tttccatacg aattgcgacc
     4501 gaaaacgata tcgatgccat tgtgcatctg atcaacgaag cgttcctcgt ggaacaattc
     4561 ttcattgagc gcgaccgcac ggatcccccg acagttcgag cgctaatgga aaaaggtatg
     4621 tttctgctgg cggaagaaga ttcgatgctt gtcggatgcg tcttttcgga actaagaatc
     4681 gagcatggct atttcggaat gctctccatc gatccgtcac gtcagggaat tggcgtcgga
     4741 cgccaacttg taaaagccgt ggaaaaatat tttacggatg cgggctgcaa attcagcgac
     4801 ttgaaaatcg tcaacgtccg aaccgaactg catgacatgt atcagcgctg gggatacgtc
     4861 gacaccggaa ccgacgtata cgacgatccg acgccaacaa aaaccccggt ccacttcatc
     4921 aatatgtcaa aaccgcttca ataatttccc agcgaatcta gccggcagcc ttgcttagcg
     4981 cgggttccga ttccgatcgt actttagatt tgctcagcgt gaaatcgtag ttcaccacca
     5041 gaaacgcctc cgctgaattt tctcctgtcg gagttttccc cgccggatgc ttttcaaatt
     5101 ccacgattag cttatccttc actccaaaca caacatcgga atcgatatat ttcgcgccct
     5161 ttttaaatag atgggtaatg agcgtctcgt atcccggagc gctaatcaga aaatgaatat
     5221 gcgccggccg gaagtgcgat atatccgttt ggcggagcaa gtcgccaacg gtaccgtcca
     5281 tcggaatgga atatcccggc ggggctatcg tgcgaatcgc atattttccg tccggccccg
     5341 tatgaaatat cgcgcgcaaa acgggctcat ccgcgcccag ctgcgattca tataaaccgt
     5401 cggcatccgc ctgccaaata tcaatttttg cgtcttcaat cggcttcccg ttcatgtcgc
     5461 gcactgtacc gacgagaaat gcaggttccc ctgcgagacc ttcggcgacg ttcgcgccca
     5521 tctccatttc cggcgaatca tcaatgtgaa aaggtccgat cacggtagac ggcgtggcgc
     5581 cctccggaaa gcggtgattc atagcgtcca ccagcatgct gatgccgagc acatcggaaa
     5641 gcagaataaa ttcctggcgt ttgtcggaac taagcttgcc cgtcttcgcc agccattcca
     5701 cggccatcag ccattcattc tgtttgagct ccacgtcgcg cgcaaatcca tgaaggtgtt
     5761 ttaccagcga ctgcataatc tggcggagtc tgggcgagtg gcacgcttcc cattgtttca
     5821 acgccagttc agttagattt tcttcggtgg catacggcat gaaaattcct cccctaccag
     5881 gcttgtatcg gcgatcttct cgccgaaaac ccctctctgt ccatagattt ctaattcgtg
     5941 ttagaaaagc ttcggctcat ttccacatcg caaattccgg cgtggaaaat cagccggatc
     6001 gaccacactt gttaaggtag acttaaattt tgtggcagcc ggactcatgt tccgcgcacc
     6061 gctccatcct gtttctgtgt gtataggatt tgaaattgga aagaagtgag gttccatggc
     6121 aatccttgta accggcggga gtggctacat cggaagcgtg accgtcgagt gtctgctggc
     6181 aaaaggcgaa gacgtcgtcg tcctggacga tctggcgcac gggcaccggg ccgctttgcc
     6241 tgcgaacatt cccttttacc agggaaaaat tggcgacgag gatctcgtcg cgcggatcgc
     6301 ccgcgaacac gccttggaat cctgcatcca cttcgccgcc atgattgagg ttggggaatc
     6361 cgtcctggac cccgcaaaat acttcg
//
LOCUS       DASZMB010000073        42767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3377240, whole genome shotgun sequence.
ACCESSION   DASZMB010000073 DASZMB010000000
VERSION     DASZMB010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 42767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42767
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3377240"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VG272_03390"
     CDS             complement(<1..378)
                     /locus_tag="VG272_03390"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HWF12754.1"
                     /translation="MNSAQFISALAVAVAVYAAVSDVKERRIPNLLTYPAMAAGLLLQ
                     SALHGWKGLLLSVGGGLIFGGVFMLFYLVRAMGAGDVKLAVALGCIVGNSALQVMFAT
                     ALAGGALAIFFMVQSGRILETLRN"
     gene            complement(452..634)
                     /locus_tag="VG272_03395"
     CDS             complement(452..634)
                     /locus_tag="VG272_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12755.1"
                     /translation="MTFKQFISNFHREESGQDTVEYVLIGAAVVTALVAGAPGLSTML
                     TNAITALGTKVTTAIG"
     gene            1227..1385
                     /locus_tag="VG272_03400"
     CDS             1227..1385
                     /locus_tag="VG272_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12756.1"
                     /translation="MKIPVEILMNGFVQEFGGKLIGKLIPIIERCESVAFSGYVMRIA
                     GELSILKM"
     gene            1919..3370
                     /locus_tag="VG272_03405"
     CDS             1919..3370
                     /locus_tag="VG272_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWF12757.1"
                     /translation="MSNDQIRVFFCSPDIALVEPLTDALGKGFQIHATREVRWPGAAE
                     SGAYDVLLLDLRASDKDPVIKAALDFLTTSAKTKFPLPVVAILSSNDRALTLRLMDLG
                     IHLILASPPDMIELRIALRRACRFHQLEKEGQELRAQEMKITASYMMVGVEGVLTEVM
                     ALAQKVAPCDVTVLITGETGTGKELWARAIHRMSSRSTQPFVGFSCANLPETLIEDEL
                     FGHEKGAFTGASNTRRGRFELADRGTLFLDEIGDLSPGLQPKLLRVVQERSFERLGGS
                     KPVAVDVRMICATQHDLHEMVEQGKFREDLFYRLNVVQMHLPPLRERRESIPELAHHF
                     LRQFSNQFGKKTKCFSDLAMQALEECTWPGNVRELENVVQRAVVLAEDSTVELSHLPE
                     KLKKGFTDSSPTHSYDLEIREFKKRLVLRTLRQCGWKKTETARTLGIARNYLHRLINQ
                     LNILSEETPKNRVGRGMESSVSFADGLSGKKCA"
     gene            complement(3397..4719)
                     /locus_tag="VG272_03410"
     CDS             complement(3397..4719)
                     /locus_tag="VG272_03410"
                     /inference="COORDINATES: protein motif:HMM:NF015078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF12758.1"
                     /translation="MAAETHTNIGPTDRPDAKNSKRELLYAFIVVFFGITAVSLSQQN
                     AIGLIPLKNLLKNELNENRAATAGFFFWLGLAWYFKPFFGIFTDAFPFLGTRRKSYIV
                     TGAILSVASFIALIYTPHRYADLLAMCIAINVFMVITSTAVGGFMTEKAQSFKASGRF
                     ASVFQIAYQTATVVGGPLGGILAATAFGWTGAASAAIMFLPIPAAIFFLKEKRINIDA
                     ERLLGNAGRQLTRIVKAKTMWAAAGFSFLFYFAPGIQTALFYRQQNELHMSTKQQGLM
                     LFLNGIFAILTAIFYGSYAAKRWNLRKLLLWCIVAGAVSQMGYVFYNSMDQAYIIEII
                     WGLGWASADMALTDLYMRATPAGSEALGFSLMISVRNLSLFGADWLGAKAMDTYHFHF
                     STMAIANGLISLIAVPFVFLLPGFIVDQRDSRAEAEAAPSAGKVLLEE"
     gene            4981..5937
                     /locus_tag="VG272_03415"
     CDS             4981..5937
                     /locus_tag="VG272_03415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWF12759.1"
                     /translation="MKMGGFGGASLRFLGAVLLFAFAGQEPAKAQDQSQGTIRVNVNL
                     VLVDATVKAKDGQIMSTLKKEDFEVREDGAVQKLDVFSRDELPVNVALVLDLSDSIEP
                     FLAPLRDAAKIALGALKPDDEVSLFTFSTEAQMRQPLTKNKNQIADQINTFEARGATN
                     INDGIFSAAEYLLKQPPNGRRVIILISDDVGTDAGGQGTRDIVTEAIASDSVLYNLKI
                     PGYNPPATIFAASMIPGLVNIRKVMDATGGEEFDVKDVANLDAVFSALIQRIKTRYTL
                     GYYTMANGAGGKPHKLDVRLVSSFGKKGRDYNILAKNGYYVH"
     gene            5998..7047
                     /locus_tag="VG272_03420"
     CDS             5998..7047
                     /locus_tag="VG272_03420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWF12760.1"
                     /translation="MIKLSVLDQSPVSDGYTPADALRNTIELARLTDRLGYERYWIAE
                     HHAIVTLASPAPEILISRIGAETSGIRIGSGGILLPHYSPLKVAETFRMLHAMYPGRI
                     DLGIGRAPGGSGLEAFALRRDRSDRAQNDDFAEQLLELLAFLHHDFPSEHPFARIKVS
                     PDMPGAPEVWLLGSSLWSSSAAAKLGLPYAFAHFIMPEETPNALENYQSNFQPSKNLL
                     KPQSVVALGVICADTEAEAKRLYTSAQLHVRRIRLQGKRLPVPTPEQAIAELGSLAME
                     TDPVVRGGGEWPRYVVGTPEQVHDELSRMASSLKVEEIMIIAVMHDYEARQRSYQLVA
                     EAFTLSGRVPKVANA"
     gene            complement(7122..8327)
                     /locus_tag="VG272_03425"
     CDS             complement(7122..8327)
                     /locus_tag="VG272_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HWF12761.1"
                     /translation="MSTTRREFIKTVGAAIASAQIAPSLAQASQPTEKRKVIYDQDND
                     GPFGTDILGILMMLQAETIDLLGITLVTGDAWMRQEMAYTLRLLEMMGRTEIPVYPGT
                     EFPMLNTREEWQLRQQLYGGHRMDPWMGAFNRTSGGPDEIKPLAPPYDRLANLKPQQE
                     HAARFIAKSVRENPGNVTVYAGGPLTNIALAIALAPDIVPLVREIVIMGTGFHVFTNA
                     FNMFFDPESARKVLRAAWPKCSIISVDIAEEIHEGDEIAPGRRMIDEIAARADSPISN
                     LFKIHAVEPLRQNPSKQFFRMADEMTAAQIIDPSVFTKSEQMYVDICTTPGPRYGDAM
                     FWPKNWQDVPDAGKTVSAADRRVFVDPRQFYLGPPPSAGLVNVLQEIDGARFKKLFVD
                     LMTKPIRSA"
     gene            complement(8423..9556)
                     /locus_tag="VG272_03430"
     CDS             complement(8423..9556)
                     /locus_tag="VG272_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HWF12762.1"
                     /translation="MHAPHNFLAHSSMFSPAPQTSSPSGGKKKVIYDQDHRGPLSTDT
                     VATLMLLQADNIDLLGICTVSCDMWAKQETAYALRLLELMGRTEIPVFMGAQEPLLNS
                     KPEVQMRYQMFGSRTLGNEGYLGCFAKDAPGRDELAPLPLPYNRFAQIKAQPEPAADF
                     IIRTIRANPNEVTMYCGGPMTNLALAAMLAPDIVPITKEVIFMGGGLHHSTSSVNVYF
                     DAEAAKIGFRAAWPKFTLVTTDLAEQVHMQDDGKVDAIVDRAQSPIADLFREYEQKPQ
                     RANPQRRSFRMPDEMMAAHVIDPSIFAGYDDMFIDVVTHNDGHYGDTSFWSANWKNQN
                     GELAGQNGPSLKAGRVQVLNGIDKKRFRELFTDLMTRPINRSR"
     gene            complement(9777..11267)
                     /locus_tag="VG272_03435"
     CDS             complement(9777..11267)
                     /locus_tag="VG272_03435"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HWF12763.1"
                     /translation="MTGTTATSTKVLNFINGNWAESKSAEWRDVMNPATGEAIASTPL
                     STAAEVDAAVETAAVAFREWRRTPAEDRIQPLFKLKMLLEEHVDDLGRIIARENGKTF
                     VEGKAELRRAIENVEVACGIPTMMQGYNLEDVARGIDEMMIRQPLGVVAAITPFNFPG
                     MIPFWFLPYAIATGNTFILKPSERVPLTMVRVFELLEQVGLPKGVVTLINGGKTVVDA
                     LIDHPKVRAVSFVGSTPIAKYVYSRAAANGKRAQCQGGAKNHVIVLPDAEMKMATQII
                     NDSAFGCAGQRCLAVSVAVTIGDAQKTFRESIAEAAASLKVGDGLDEGVQMGPVITSQ
                     SKSRIESLIGVGEKEGAKVILDGRNAKIPKHEGGNFLKPTILDGVPSTSELTDTEIFG
                     PVLSLVHARDMDEAIAFLERSPFGNQASLFTSSGSAARRFRYEAPAGNIGINIGVAAP
                     MAYFPFTGWKGSFFGDMHAQGRDAIEFYTDKKVVIERWGDQHSRKF"
     gene            complement(11365..12327)
                     /locus_tag="VG272_03440"
     CDS             complement(11365..12327)
                     /locus_tag="VG272_03440"
                     /inference="COORDINATES: protein motif:HMM:NF040065.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF12764.1"
                     /translation="MKVGVAGQGAFGQKHLEGIAKIPGIEVITLTGGNQAATEEIAKK
                     FKIPHWTNDLGESLKQPGLEAMILATPTQLHAKQGEQCMRAGKHVQIEIPIADTLADS
                     ERLVKVQKETGVICMGGHTRRFNPSHQYVHKKITAGELKIQQMNVQTYFFRRTNMNAA
                     GKPRSWTDHLLWHHACHTVDLFQYQTDELVSECYGVQGPIHPELKIAMDMGIVMKTPS
                     GAICTLSLSFNNKGPLGTFFRYICDNGTYIARYDDLLDGDENKIDVSNVDVSMNGIEL
                     QDREFFAAIQGKREPNASVAQCYTAMQVLDRIERFLNSASKTSA"
     gene            complement(12609..13658)
                     /locus_tag="VG272_03445"
     CDS             complement(12609..13658)
                     /locus_tag="VG272_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12765.1"
                     /translation="MLPLPLRLSERWRAFYKFSGEAKHFSINNPLHIPPKQFLAVITL
                     GLTLFFILCSTRAQDTATAAGDSVQMIVSVEPRHGNQIPMITQQDIMVYQGHDRLPVT
                     GWIPAKGEKAGLQLAILIDESAGFSLGAQLNDIRSFISQQPPSTLIAVGYMQNGTVNL
                     ASDFTSEHAATTKSIRLTQGYFGAEGSPYLSLSTFIKGWHSNSPAIRREVLMITSGID
                     NVYMGNIDNPYVDAAIQDAQCAGVVVYSIYTPSAGHMGHSYYRTYWGENYLSELAEAT
                     GGESYYLMGPQAPVSFSPYLKKLNEQLPNQFLLSFQPKTEKKAGLQSVRVTSEIHDVD
                     FLHQDKVCIPAAPKE"
     gene            13979..14767
                     /locus_tag="VG272_03450"
     CDS             13979..14767
                     /locus_tag="VG272_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWF12766.1"
                     /translation="MRRIFLSFAAVAILLGVSAQAFAQTEVTLIAPGGIRAPIEKLIP
                     GFEKKTGYKVNPTFGSGGGTKKQVISGDVFDVPIVQPPLAEVTASGNVVESTATPLAS
                     VAVGVAVKKGAPKPDISSSEAVKKMLLSAKSIVCPDATTGAAAGVSINDMLKKLGIDE
                     QVEPKLKRVRGTGAGGAGGGTMVSGMIANGEAELGMTFVSEMTDPGIDVVGVLPKEVS
                     PWTPLVGYISTHSKNPEAAKALLKYLSSPEALAAYKAAGMQPGK"
     gene            complement(14816..16171)
                     /locus_tag="VG272_03455"
     CDS             complement(14816..16171)
                     /locus_tag="VG272_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12767.1"
                     /translation="MARSSSRNDVTAIPFGINTPTIFAYISLIMLPVYERTHDANLAW
                     QMGIFASLVSGIVQSVSAFCIDWMRRTTPLAALLCPLAGLAMAFLCLGFIFGIFEIPA
                     VALLPAVILLATYASKMKLPWRIPSGLVAMVVGGIIVVILRAFHVYHLPPQPPLLPPG
                     IYLPRPVNLYQIIVKAEGWRYASIFLPLSLLDTIVSLQILESVRVAGDDYPTNPSLLV
                     NGLATIVGAALGSAFPTSLYIGHNAHKANGARIGYSILGGVVSAALCLTGLVPAVLRF
                     VPLEVVAFVVVWFGLAMVGQAFQEVPVAHSVAVALGLVPMLAQWVTQIVDLAIRKAGA
                     DMLTVAPRFGGELAIYGMIALGQGALLVSMIWAAALVWIIERRFLQSAAWMLVAAVLS
                     CFGVIHAYQLTSQGIENHLGWWVAPEFSLSYLAAAVFLLVCHLYAQNRPNAFLNSDLE
                     G"
     gene            complement(16439..17434)
                     /locus_tag="VG272_03460"
     CDS             complement(16439..17434)
                     /locus_tag="VG272_03460"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HWF12768.1"
                     /translation="MPQDVGLNDPLLLNEWFAVAWTGSLPENKLQPVQALGRDLVLWR
                     STSGVHAWKDLCIHRGAKLSLGRISKSEKSECVVCPYHGWEYDSTGACVRIPAHPDQA
                     PPPRAHVDCYEVREKYGLVWVCLGKPVGEIPSFPEGETPGFRLISTGPYAFHAQGPRI
                     IENILDLAHLPIAHAGMLGDPSKAEIGEYAVTTTAEGIVARDIPLWQPDPDGTGRGAN
                     VHYTYWVERPFTTRLTKTHPTQHFAILGTVTPIDDENTLAWVVIAMDYAHEVPEKELR
                     EFQDKVTAQDICIVNSQRPELLPLDLQSELHLRSDQIAIAYRRWLKQLGLQYGTS"
     gene            complement(17620..18864)
                     /locus_tag="VG272_03465"
     CDS             complement(17620..18864)
                     /locus_tag="VG272_03465"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HWF12769.1"
                     /translation="MPNRRDFAKLVLAGGAGMLAPRGLWSGAAGPPAAPQNANGKFDL
                     LIEGGTVIDPSQNLNGLMDVAIKDGKILEVSKNIAKDRALKTVSAKDRLVTPGFIDMH
                     AHCYDGVGIGMNADRYCLNRGVTTMLDAGSAGTATFPNFKKYIINTSTTRIVALVHIS
                     PIGTIMYEGGLSNLSWLNPQATAKMATDNKPVTAGIKIQLSKPITGSNDLECLKRALQ
                     AAEIAHMPLMAHIEDSFSPLPDILKLLRKGDVFTHCYNNRAHSILDANGKIIPEARDA
                     RERGIIFDPAQGQTHFSFDVAEKCMAQGFLPETISTDLTIVTIERRVFDLPTMVSKFL
                     SIGLPLDKAIAMVTINPTRVFDFGAQIGTLRPGSEADVSIYELREGKFDFEDSNGIKR
                     TGQKMLTSKAVVRRGQLFINAV"
     gene            19009..20085
                     /locus_tag="VG272_03470"
     CDS             19009..20085
                     /locus_tag="VG272_03470"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HWF12770.1"
                     /translation="MNIHQSTTSYEKWVSSCIPLDRHAIVFKHKQMATGLFPFLRATF
                     YRWMQIWREEVGDAAIAPSLLAVGDLHLENFGTWRDAEGRLIWGINDFDETYRMPYTM
                     DLVRLATSAHAAAVEEHIAVRPRDASDQILEGYRDALKEGGRPFVLAENHRWLRKMAL
                     SRLRDATVFWQKLERMQKSPHRLPRHVESHLRKLLPDRSMPCEKKRRLAGLGSLGHTR
                     VVLLGKWHDCYVAREAKQLTPSACVWARGKNAEGHLARYGEILDHAVRVPDPFVHPYD
                     GWLLRRLAPDCSRIELESLPESRDEDRLLYSMGWETANIHFGAPRAIRAIKRDLAKRP
                     ANWLHKAAKAMLHATKRDWCDWVA"
     gene            20483..23560
                     /locus_tag="VG272_03475"
                     /pseudo
     CDS             20483..23560
                     /locus_tag="VG272_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014489.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
     gene            23711..23926
                     /locus_tag="VG272_03480"
     CDS             23711..23926
                     /locus_tag="VG272_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWF12771.1"
                     /translation="MPGQVGSPIPGAISSIVVEVGEQVKKGDRLLVLEAMKMQSTVYA
                     PVGGKVAKKLANVGDKVEAKDLLLVLE"
     gene            23983..24606
                     /locus_tag="VG272_03485"
     CDS             23983..24606
                     /locus_tag="VG272_03485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HWF12772.1"
                     /translation="MRKPQKKASLRIKRLQRTDLPTDTMDLAAYLIGKTLVHDLPRVR
                     LSGRIVETEAYPVGDMAAHAFRGETPSIRSLFLPHGHAYVYFTYGSCFMMNVSSEEVG
                     TGAGVLVRALEPLEGIKFMERRRDTIRLHDLTRGPGRLAQAMDINKRHDGLNLCAGGK
                     LWLGDAVRPAGPIGISIRIGITRDVHRPCRFYERGNPFVSGLKRMRE"
     gene            24767..25033
                     /locus_tag="VG272_03490"
     CDS             24767..25033
                     /locus_tag="VG272_03490"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HWF12773.1"
                     /translation="MATQEACPHVKEIKNVKPSANGCEECLKTGDTWVHLRMCLICGH
                     VGCCDSSKNKHATKHFHRTKHPIIQSIEPGENWRWCYVDEIEVD"
     gene            25074..26000
                     /locus_tag="VG272_03495"
     CDS             25074..26000
                     /locus_tag="VG272_03495"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HWF12774.1"
                     /translation="MSAAKTVLDSSEAWPGLPLSAWAETYATLHMWTQIVGKIRLAMS
                     PHMNHWWECPLYVNARGLTTSPIPYAKGAFEIQFDFIDHKLDVTSSSGETKSMRLQPC
                     TVADFHTEIFVLLKSLGIEAKIWNMPVEIPNPIRFDQDRIHNAYDPQAVNAFYRILVN
                     VDCIFKEFRSRFLGKVSPVHFFWGSFDLAVTRFSGRRAPDRPAADKITRDAYSHEVSS
                     VGFWPGGGDINGAAFYSYAAPQPEGFGERPVRPTAAYYHKQMGEFLLMYDDVRASDSP
                     RSALLDFCQSTYDAAAVLGNWDRANLEKPVVK"
     gene            26312..28651
                     /locus_tag="VG272_03500"
     CDS             26312..28651
                     /locus_tag="VG272_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWF12775.1"
                     /translation="MAHFIRIIAFVTYACVCALPMLAQQDRGELQIEVHDPQGTLLSA
                     SGELVSEGNQFHLTFTVDDEGRFIARGLAFGIYRVSVSHSGFVSAVQLVEIRTIVPKH
                     LSLTLGLKPVETQVQVSETETLVDPTRTNTVYTIGSQTIDEQLSSRPGRGVLDVVNAQ
                     PGWVYEANGVLHPRGSEYDVQFVVDGLPMTENRSPGFAPPIESSDVESMRVMTAGFPA
                     EYGRKLGGVVEITPPKNTPAGFHGEFEAEGGSFDTGSASGALFYSIGGNRFSISGDAF
                     HSDRYLDPTVLQNYTNIGNSGGFSTSYERDFSNGDRLFFSLGQHAVRYEVPNELVQQV
                     AQQRQNSEQKETSGQGRYTHAFSTDIFFSIAGSIRDASAFLNSNAQSTPIVVSQNRGY
                     REGYIRADLAGHHGRHDWKFGADGIFSPVNENLQYRITDLSQFDPGTALSFQFSDRRW
                     DIEPSAFVQDQIRFGKWNVSAGLRYDHYGFAVSKSAWSPRLGVSRFISSLDLLVHASY
                     DRVFQTPAMENLLLASSPQLDSVSNFVVRLPVQPANANYYEIGFTKSFSGKLRIDANI
                     FRRDFHNYPDDDVLLETGISFPISFSTATIHGEEIQLSLPHWGRFSGVLSYSNQTGIG
                     QGPITGGLFLGSETAGIPDTSKFAVSQDQRNTVRARVRYQATDRLWFATSAEYGSGLP
                     ADLNGPADPSQFNFLLQQYGQAILNEVNFDAGRVRPNFALDAAAGATLYHKEGKDVSF
                     EIEGHNLTNKLNVVNFASLFSGTAVAPPASVSARLKFGF"
     gene            28948..30792
                     /locus_tag="VG272_03505"
     CDS             28948..30792
                     /locus_tag="VG272_03505"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HWF12776.1"
                     /translation="MNRPNTRLSVRRTRWRTYCLGAIFTLLAARAAADTNNADPKRYL
                     DDVKALSSPAMEGRGAGTKGIDLAANMIEQRYRSLGLQPAGSKVFLQPFTVITGAKLK
                     EGNRIEIEDRKSKRGLKLNQDFVPFSFSASGELSGPIVFAGYGVTAPEFGYDDYAHLD
                     VKDKIVVILRYEPSGFAAKSGNAGLTQHSQLITKAINARNHGAKAVILVNGKVRDGEE
                     DQLTKFGSVNGPENVGTMIVQVKNDAANAWFIDAGKSLVDIQSQIDSSSTPSSFAFPD
                     KLQISLGVNIEKTRATVNNVLAYLPGKTGEYVIIGAHYDHLGYGNIDSLAPSQIGKIH
                     PGADDNASGTAGVLELARLLAPRKGQLQRGVLFADFAGEELGLLGSAEWVKEPTLPLD
                     KAVAMINMDMIGRIKDEKVFIGGTGTGAEFKDLLNEEKSKSPFHFEYSASGYAASDHT
                     SFVGKQIPVLFFFSGLHSDYHKPSDTWDKINAPDAAKLLDFIEDVVLKVDSEKERVAF
                     VTVKEDQPEGHAASGGGGYGPYFGSIPDFGQEENGVKFSDVRPGSPAAKAGFKSGDIL
                     IQFGDKPIHNLYDFTDALRRSKVGDEVEVKVSRDGKPVSATVKLEQRK"
     gene            30976..32079
                     /locus_tag="VG272_03510"
     CDS             30976..32079
                     /locus_tag="VG272_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12777.1"
                     /translation="MKKSQFVRPALFLLLFTAAASLATYSQAPVQGKSSLDSLTVPGE
                     EHLGNVRQLTFGGTNAEAYFSADDKSLIFMHSGIGVPCDQMYTMQIDMPGGKPSVPKL
                     VSTGKGRTTCGYFFPKGDRILFSSTHETSAECPPRPDYSHGYVWPIYSSYRIYTAKPD
                     GSDLRLLNYAPGSYNAESTISRDGKKIVFTSTRNGDLDIYTMNADGSDVRQLTHELGY
                     DGGAFFSDDGKKIVYRSEQPKTPEQIADYNELLSRGLIRPGNLEIWVMDADGNNKHQI
                     THNGAANFAPYFFPGGKRIIFASNMANQKDPSGFDLYAINVDGTGLERITFHPDFDAF
                     PMFSSDGKRLVWASNRNGKAPHETNIFIADWVE"
     gene            32472..33596
                     /locus_tag="VG272_03515"
     CDS             32472..33596
                     /locus_tag="VG272_03515"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12778.1"
                     /translation="MYRSDVIGSLLRPTYLKKAREEFAAGHLSDAEFKRIEDRAVDES
                     VALQLRAGLDVLTDGEMRRYAFYGHMIDAIEGFDKYGGWSIPFRNEKGEETNHPRPVV
                     VSKLRRKRHMCAEEFTYLRARTDHTAKTTLLSAQQAAAYYDAKKSSGVYPTVDSFLAD
                     LVDLLREEVAELVRLGCTYIQVDSPQYAALIDSSIREGYRQRGNDPDRLLDLSIEMDN
                     AVIAGHPHVTFGLHLCRGNNQSKFYASGDYAPIAKVFRETKFQRFLLEFDDERSGGFE
                     PLSHVPSDRTVVLGLISTKKAPLEAQDEIKSRIHAAEKYVPFDRLALSPQCGFASTME
                     GNLLSEADQEAKLRLVVETAREVWGEEKSSLSSKVKGKSV"
     gene            33598..34929
                     /locus_tag="VG272_03520"
     CDS             33598..34929
                     /locus_tag="VG272_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWF12779.1"
                     /translation="MNLPPGPKGFPVLGSVFEPKGDTITYLTKCTREYGDIVFFRFLG
                     VPACYLSRPEYIESVLVTQNNNFVKSKDYRAMRRVLGNGLLLSEGDFWRRQRKLIQPA
                     FHQEKISAYAQIMVDYTQRMLETWNDGQALDMHEAMMRLTLGIVAKTLFDADVSHEAE
                     DVDAALTVLMGKFLRQAGMALLLPTWVPLPTSQLLKRAVGRLDKVIYGIIEQRRASGQ
                     MSGDLLSAFLQTRDDEGVGMTDRQLHDEIMTLFLAGHETTANVLSWTWLLLGQNPEVE
                     QKLIEELNRVLNGKIPTPADLPRLVYTDMVLRESMRLYPPVWVIGRRALSTFRLGDYE
                     IPANTNMLISQYLMHRDALYFPEPERFDPSRWSPTDPRAASLPRFAYFPFGGGPRVCI
                     GAGFGMMEAVLLLATIAQKFQMEVAPGEKVSMQPSVTLRPRNGIPMKLKRR"
     gene            complement(35009..36016)
                     /gene="rfaD"
                     /locus_tag="VG272_03525"
     CDS             complement(35009..36016)
                     /gene="rfaD"
                     /locus_tag="VG272_03525"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR02197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HWF12780.1"
                     /translation="MTFVNENWKDKRVLVTGAAGFIGSVLVWELNRRGCTQVMLADFD
                     VNGEKRFNLAGLQYTEFILVPELHARLASGSLGHLDCIFHLGACSSTTETNETYLREN
                     NFEYSRRLAEWALGAGVRFVYASSAATYGDGSAGMDDTDVIQMQQLKPLNLYGQSKQW
                     MDLHALQEGWLDRIVGLKYFNVFGPNENHKGDMRSLVCKSYADVLKTGVIRLFKSHRP
                     EYRDGEQRRDFLYVKDAVDMTLHLAANPNANGIFNIGSGQARTWNDLAHAVFTALGRE
                     PHIEYVDMPEVIRDKYQYFTQADIQKLLATGYDQPITSLEDAVRDYVVNYLVPGRTIG
                     V"
     gene            36151..36696
                     /gene="hpt"
                     /locus_tag="VG272_03530"
     CDS             36151..36696
                     /gene="hpt"
                     /locus_tag="VG272_03530"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HWF12781.1"
                     /translation="MHGHGQEHLKVLLRSSAIQKRVREMGAQISKDYKGQRVHLIGVL
                     KGAAIFLSDLVRQIKVEVSLDFMSVSSYGHGSQTSGQVRLNKDLDTSIEGLNVIVVED
                     ILDTGLTLGYLLRVLQQRRPHSLKIAALLDKPERRIKKDVKADYVGFQIPNEFVVGYG
                     LDYAERYRNLKDVCVLSLPPS"
     gene            36778..37440
                     /gene="rpiB"
                     /locus_tag="VG272_03535"
     CDS             36778..37440
                     /gene="rpiB"
                     /locus_tag="VG272_03535"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HWF12782.1"
                     /translation="MKPLITAADLERVPENGELAVTDDTIVTPLARDAAERRGITIRI
                     ASQREQQSSANLSAGDISSEKSARIVAIGSDHGGFELKEQLKGHLRDWGYQALDFGTN
                     SSEAVDYPDFAETVGNAVAKGEAWLGIVLDSAGIGSSIAANKVPGVRAALCYDRATAR
                     NSREHNDANVLTLGAKLIAPEAAREIVALWLSTPFAGGRHQRRVDKIRAIEERAAKRQ
                     SE"
     gene            37567..38502
                     /gene="deoC"
                     /locus_tag="VG272_03540"
     CDS             37567..38502
                     /gene="deoC"
                     /locus_tag="VG272_03540"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HWF12783.1"
                     /translation="MDQSEFEQIVRIVTEEVVRYLGASGNANVQRAANSDSEICPDCD
                     LNCAEKCARKTRAVVEAGADRISCGPSVVELESGFAGLIDHTLLKPDASRDDIRKLCQ
                     EAVRFGFASVCINPWNVPLAAELVRGTKVKVCTVIGFPLGATLSQVKIHEAEEAIKVG
                     AQEVDMVINIGALKSGQDDVVESDIRGVVEAAHRGGAICKVIFETSLLTVEEKVRAAL
                     ASKRAGADFVKTSTGFSTGGATAEDVAIMCAIVGSEIGVKASGGVRTFDDLKKMVCAG
                     ATRIGASASVKIMEQAAGPASSPALTSGVPASSSY"
     gene            38864..40630
                     /locus_tag="VG272_03545"
     CDS             38864..40630
                     /locus_tag="VG272_03545"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWF12784.1"
                     /translation="MNYLIMDPVQTPTPLNVPAEVLATLVEIGEEINSSLNLDEVLKK
                     TAALVKRLIDFEIFSVLLVDESMHRLYHRFTIGYGEEAGKEWVIPIGQGITGTAAATG
                     RAVRVPDVREDPRYINIIDSVRSEMVIPLMVKGQAIGVLDIQSTQVDYFTRDQQSVLT
                     LMASRLATAIENARLFERIRSQADTLLVLNEVGREANSILAVEELLRRAAELVKRVID
                     YQILAILLYDDAANIFRHRLDMKYGQSAQGKLRISPTEGIVGTAISTQMPVRIPDVTL
                     DSRYIMVNPETRSEMAIPMIHKGRVVGVLDLESPQINYFTDDHEQTLTILAAHLAVSL
                     ENARLYEQLAKDEARMERELTAAQRLQTALLRPVPTTDYGLDITARIHSAREVCGDLY
                     DFLSYGTQNLGIALGDVSGKGTAAALYGAVATGILRSLSPQKLQPAELLRLLNQLVCD
                     RRVEGRYMTICFATWQKGRRKLRVANAGQTQPLLWKDGHVEQIKLSGFPIGIFDDATY
                     EEWSVCLSSGDILLFFSDGLTEAANREGKFFGTNRIKDLLAANSHMNSSDLADRLLQE
                     VEEFTQGGAITDDRTLVVVKVK"
     gene            40627..41934
                     /gene="lysA"
                     /locus_tag="VG272_03550"
     CDS             40627..41934
                     /gene="lysA"
                     /locus_tag="VG272_03550"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HWF12785.1"
                     /translation="MSASASYSSASRAAHAAIVDPVTYTPGFSYRGSQLYCEGVSVAV
                     VAKKFGTPAYLYSRASIEQAYRRMDSAFGAIPHTICYAAKANSTLSILRVFAAMGSAF
                     DIVSGGELYRLRRIGVSGNRIVFSGVGKSREEIREALRARILLFNIESSAELDLLASE
                     ASRLRVRAAASIRVNPDVKAGGHRHIATGHHSHKFGIDWGTARDLYLQHRGSRWIDWQ
                     GISAHIGSQILSVNPHRRALKRLAGFVSDLATAGIPLKYVDCGGGLGIRYTSEQPPET
                     DVWAAELVRVIKPLGCHLLIEPGRSLIGPAGVLVMQVLYNKETRGKNFVIVDGAMNDF
                     MRPALYDATHPITRARRSSGSRDRADIVGPVCESGDCFVQDWPLGKVKPKDVLILWGA
                     GAYGTVQASNYNSRPRPAEILVSGNGMKLIRRREARGDLVRGE"
     gene            complement(42117..>42767)
                     /locus_tag="VG272_03555"
     CDS             complement(42117..>42767)
                     /locus_tag="VG272_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12786.1"
                     /translation="ARRTAATIADIMPTNLFRPIGLRELELLWDSGMKEFPQRLENQA
                     IFYPVVNLEYARQIARDWNTPDAKSGFSGFVTKFEVSSTYLSKFELHTAGSAAHREYW
                     IPAKELSVFNKAINGQISVEEGFFGSEFVGYQEEENGFKDFNAIEQFSALSKMAGQED
                     FSSQVSVHRKAIFMNCLFWLNTDISNLPCTKEQRDTFLTALVNAWEADSIGVPLPI"
BASE COUNT        10092 a        11078 c        11661 g         9936 t
ORIGIN      
        1 gttccgtaac gtctcaagaa ttcggcccga ctgcaccatg aagaaaatgg ccagcgcgcc
       61 cccagccagt gccgtggcga acatcacctg caaggctgaa ttccccacga tacagccgag
      121 agccacagcc agctttacgt cccccgctcc catggcccga accaggtaga acagcatgaa
      181 caccccaccg aatatcaagc cacccccgac actgagcagt agccccttcc agccatggag
      241 cgcggactgc aatagcagac cagcagccat ggcgggataa gtcagcagat tggggatccg
      301 ccgttcctta acatcactga ccgcggcgta aactgccacg gcgacggcca gagcggagat
      361 gaattgagca gagttcatag tttcattctc aaatgaaaag agggggcgcc gtgccttatg
      421 ggggagcatg ccgcccggcg cctctcctgt gttacccaat cgctgtcgtc accttagtgc
      481 caagggcggt gatcgcattg gtcagcatcg ttgaaagacc gggcgcgcct gctaccaggg
      541 cggtcactac ggccgcgccg atcagtacgt actccactgt gtcctgaccg gactcttccc
      601 ggtggaaatt gctgatgaac tgtttgaacg tcattaatat ctctcctttt ctaggttgat
      661 ttgggtgccc acaacgtggc ccttggataa ggccattcgc agtgagtatc ccctcttggc
      721 tcgatacaca gcctctgtag tgcacgctgt ggaccgaatc gggtccgggt gtaagtgact
      781 gaaactattg tcaagttgga cacttaagga aaaattggag ggaaatgtct tgcgcatttt
      841 cggaaaaacc ccctccgatg gacgctgcgg tggcggaatc caggagcggc ttcattccca
      901 gctagcgaag agagtccggg tatccaagtg gcagacttgt ttgcgtgccg agcacggagg
      961 tacgccgttg aaaagctgcc aaagcatgac gccgcgcgca aaccattgct tcaaacacgt
     1021 ttaccgcgca tcggaggcac ttctgactgc cttttcattg cggatatatc tgaaggggta
     1081 gacgagattc cgccgtatct ggccagtgaa caatcaggat aagcagggcg cgagccgctg
     1141 aaacatggaa tgaatcgaag ttcctgattc tgcgaaacag ggatgtagcg gaacgcggga
     1201 gtcgttagga ctcgaatgga aacaccatga agattccagt cgaaatttta atgaacgggt
     1261 ttgttcagga attcggcggt aaactgattg gaaaactcat accgattatc gagagatgcg
     1321 aatcggttgc gttcagcgga tatgtaatgc gaattgcagg tgagctttca atattgaaaa
     1381 tgtgaattcc gatgaaagca aaattggcta aaaacttttt gtgtctttca agagacatgc
     1441 gactcgaaaa gatcgaccgg cgtgaagatt tatctcaaat gatttttgta atcaaacgcg
     1501 agcacgttaa aaagtaaaat ccaaaaaata gacgcaaaat cgctagtacc gatggtaccc
     1561 gctcagatcc tgcacaggca gttttcgcca tgctggttgg cagctttcct ttataaatca
     1621 ctacaaacac agtatctata aattcccgcc cttggagagt cgtgtcctgg agacaaaaga
     1681 gaatgccgga tgtcttcgat tgagtactat gaatttggat tagaaaaatt gttacttgtc
     1741 ttaaattggg cccggtcgta tacatgtgcc tcgtcaggac gtccccctgg ctggaaaaaa
     1801 acagaacatt ttcaaggtaa tcggaatgct ttcttgcgtc atggtgtctg ttcagaatta
     1861 atttgcggaa aaaatccgca tttgaaaacc aacgccagcc ccgggggtca gttcgcttat
     1921 gagcaacgat cagattcgag ttttcttttg cagcccggac attgcccttg tcgaaccttt
     1981 aacagacgcg ctcggcaagg gctttcaaat tcacgccacg cgagaagttc gctggccggg
     2041 cgcggcggaa tctggcgcct acgatgtctt actgctcgat ctgcgcgcga gcgataaaga
     2101 cccagtcatc aaagctgccc tggatttcct gaccacctcg gctaaaacca aatttcctct
     2161 ccctgtagta gctattcttt cctcgaatga tcgagcgctg accctgcgcc tcatggatct
     2221 cggcattcac ctaattcttg ccagcccccc ggacatgatc gaacttcgca tcgctttgcg
     2281 ccgcgcatgc cgcttccatc aattggaaaa ggaaggccag gaacttcgcg cgcaggaaat
     2341 gaaaatcacg gcgagctaca tgatggttgg tgtggaaggc gtgttgaccg aggttatggc
     2401 cctggcgcag aaagttgcgc cctgcgacgt caccgtgctg ataacgggcg aaaccgggac
     2461 aggtaaagaa ttgtgggccc gcgccattca ccgcatgagc tcgcgatcga cccagccttt
     2521 tgttggtttt tcctgcgcca acctgccgga aactctgatt gaagacgaac tcttcgggca
     2581 cgaaaagggc gcctttaccg gcgcttccaa cacgcggcgt ggtcgctttg agcttgccga
     2641 ccggggcacg ttgtttctcg acgaaattgg agacctcagc ccgggcttac agccaaagtt
     2701 actgcgtgta gtgcaggagc gcagctttga acgcctcggc ggctcgaaac ccgttgcggt
     2761 ggacgtccgc atgatttgcg ccacgcagca cgatctgcac gagatggtgg agcagggaaa
     2821 attccgcgaa gaccttttct accgtttgaa tgtcgttcag atgcacctgc ctccactgcg
     2881 agagcggcgc gaaagcatcc cggaacttgc gcatcatttt ttgcgtcaat tttccaatca
     2941 attcgggaag aagacgaagt gtttctcgga tctcgccatg caagcgctgg aggaatgcac
     3001 gtggcccggc aatgtccggg aacttgagaa tgtcgtgcaa cgcgctgtgg tgctggcgga
     3061 agactcgacc gttgaactca gccacttgcc cgagaaattg aaaaaggggt ttacggacag
     3121 ctcgcccacg cattcctacg atttggaaat tcgcgaattc aaaaagcgac tggtcctgcg
     3181 aacgctgcga cagtgcggct ggaagaagac agagacggcg cggacgttgg ggatcgcccg
     3241 gaactatctg caccgtttga tcaaccagct gaatattctc tctgaagaga cgccaaagaa
     3301 tcgcgtgggc cgcgggatgg aatcttctgt ttcattcgcc gacggtttat caggaaagaa
     3361 atgcgcataa tgcgggcgac agcgcaggga acggagttac tcctccagca gcactttgcc
     3421 cgcggatggt gctgcctctg cttccgcgcg ggagtctctc tgatctacga tgaatccagg
     3481 cagcaggaaa acgaagggta cagcgatcag cgaaatcaag ccgttcgcaa ttgccattgt
     3541 gctgaagtga aagtgatacg tatccatcgc tttcgcgcca agccagtcgg cgccaaaaag
     3601 cgaaagattt cggacgctga tcatcaacga gaaaccgagc gcctcgctcc ctgctggggt
     3661 ggcacgcatg taaaggtcgg ttaatgccat atccgcggac gcccagccca agccccagat
     3721 gatttcgatg atgtacgcct ggtccattga gttatagaag acgtagccca tctgtgagac
     3781 agcgcccgcg acgatacacc aaaggagcag cttcctgaga ttccagcgct tcgctgcata
     3841 actgccgtaa aagatggcag tcaaaattgc aaaaatgcca ttcagaaata gcatcagacc
     3901 ttgttgcttc gtgctcatgt gcagctcatt ctgctgcctg tagaacagag cggtctgaat
     3961 tcccggcgcg aagtaaaaaa ggaagctgaa tccggccgcc gcccacatcg tctttgcttt
     4021 cacaattctc gtgagctgcc ttcctgcgtt gccaagtaat ctctccgcat cgatatttat
     4081 gcgtttttct ttgaggaaaa aaatggctgc tggtattggc aagaacatga ttgccgcgct
     4141 agccgcaccc gtccatccga atgccgtggc ggccaaaatt ccgcccagcg gcccgccgac
     4201 gactgtggca gtttggtagg cgatttgaaa gacagacgcg aagcgcccgg atgccttaaa
     4261 cgattgagcc ttctccgtca taaagccgcc gactgctgtg ctggtaatca ccatgaaaac
     4321 attgatggcg atgcacatcg ccagcaagtc ggcgtatcgg tgtggagtgt agatgagtgc
     4381 tatgaaactg gcgacggaga gtatggcgcc tgtcacgata tagctcttgc gccgagtgcc
     4441 cagaaacggg aatgcgtcgg tgaagattcc aaaaaatggt ttgaaatacc atgcaagtcc
     4501 gagccaaaag aaaaagcccg ctgtcgcagc gcggttctcg tttagttcgt tcttcaggag
     4561 atttttgagt gggatcaagc cgatggcgtt ttgctgacta agactcaccg cggtaatccc
     4621 aaaaaaaacg acgatgaagg cgtacagcaa ttctcgcttg ctgtttttcg cgtctggacg
     4681 atcggtcggc ccgatgttag tgtgggtttc tgcggccaat ggtttttcgt cagcgcccgc
     4741 gttttggctg gtcgctgcca ccaattccat catatcaact tagacgaaag gctctatgtc
     4801 tgttcagcaa tgctggtccc aatctggcaa cttgctctgc cgcgcgacct aggcaacttg
     4861 cgcgcccctg cgcacataga taaagtgaga tgaggcgtta tcacttgaga tagacgttgg
     4921 gaagacgcag ttggtatctt cgaaatccac aactgcaatg gtatccggga gactgaatca
     4981 atgaaaatgg gtgggtttgg aggagcgtct ctgcgctttt taggagcagt ccttctgttc
     5041 gcatttgcgg ggcaagagcc ggcgaaagcg caggaccaga gtcagggaac gattcgcgta
     5101 aacgtgaacc ttgtcttggt cgatgcgacg gtgaaggcga aggacggcca aatcatgtcc
     5161 acgctgaaga aggaagattt cgaggtgcgc gaagatggcg cagtccaaaa attggatgtt
     5221 ttcagccgcg acgaattgcc ggtgaatgtt gccctcgttc tggatttgag cgactcgatt
     5281 gaaccgttcc tcgcgccttt gcgcgacgct gccaaaatcg ctctcggggc gcttaagcct
     5341 gacgacgaag tatcgctgtt taccttttct acggaggcgc agatgcgcca gccgcttaca
     5401 aaaaataaaa atcaaattgc ggaccagatc aatacattcg aggcgcgtgg cgccaccaac
     5461 atcaacgacg gcattttctc tgccgccgag tatctgctca aacagccgcc gaatggtcgc
     5521 cgcgtcatca ttctaatcag cgacgatgtt ggcacggatg caggcgggca gggaacgcgc
     5581 gacattgtga cggaggcgat tgcttccgac tcggttttgt ataatctgaa aattcccgga
     5641 tacaatcctc cggcaactat ttttgctgcc agcatgattc ccggcctcgt aaacattcgc
     5701 aaagtaatgg atgcgacggg cggcgaagag ttcgatgtaa aggatgtggc gaatctggac
     5761 gccgttttca gcgcgctgat tcagcgaatt aaaacgcgct acacacttgg ctattacacg
     5821 atggcgaatg gagcgggcgg aaagccgcat aagctcgacg ttcgtctcgt ttcttccttt
     5881 ggaaaaaagg gacgcgatta caatattttg gctaaaaacg gctactacgt tcactaggaa
     5941 aatgtaacct tgccaacgat tagaagtgcc ggacgctctg cgagcagaag tgaggcgatg
     6001 ataaagcttt cggtactcga ccaatcccct gtatctgacg gatacactcc ggcggacgcg
     6061 cttcgcaata ccattgaact tgcgcgcttg accgatcggc ttggctacga gcgttactgg
     6121 atcgcggaac atcatgccat tgtgacgctc gccagtcccg cgcctgaaat tttaatctcc
     6181 agaattggcg ctgaaacttc aggcattcgt attggatcgg gcggtatatt gctacctcac
     6241 tacagcccgc tgaaggttgc agaaactttt cgcatgctgc atgcaatgta tccgggacgc
     6301 atcgatctgg gaatcggccg cgcaccaggc ggcagcggtc ttgaagcatt tgccttacgc
     6361 cgcgatcgca gcgaccgcgc tcaaaacgat gatttcgcgg aacagttgct cgagctgctc
     6421 gcatttctcc atcatgattt tccttccgaa catccctttg cgcgcatcaa ggtttcgccg
     6481 gatatgcccg gtgcacccga agtctggctc ctgggatcga gcttgtggag ttcatccgct
     6541 gcggcaaaac tcggtctgcc ctatgctttt gcgcatttca taatgcctga ggagactccc
     6601 aacgcgctgg aaaattacca atctaatttt cagccatcga aaaatttact caagccgcaa
     6661 tcggtcgtcg ctctcggagt gatttgcgcg gatacggaag ccgaagcgaa gcgcttgtac
     6721 accagcgctc agctgcatgt tcggcgcatc cgtctacagg gaaagcgtct tcccgtgccg
     6781 actcccgaac aagccatcgc ggagctagga tcgctggcga tggaaacgga cccggttgtg
     6841 cgaggaggcg gagaatggcc tcgctacgtg gtgggaactc ccgaacaagt gcacgatgaa
     6901 ctttcgcgaa tggcaagcag cttgaaagtc gaggagatca tgatcatcgc cgtgatgcac
     6961 gattatgaag ctcggcagcg gtcctatcag ttggtggcgg aggcgtttac tctgagcggg
     7021 cgtgtaccaa aagttgctaa tgcgtgattt caggcaggat taagatttct aatagtgttt
     7081 aggcgctcaa gaggccgctg tacattgact cgaatcgttg ctcaagcact gcgaattggc
     7141 ttggtcatta aatcgacaaa caatttcttg aatcgggcac cgtcgatttc ctgcaatacg
     7201 tttaccagac ccgccgatgg cggcggcccc aggtaaaatt ggcgaggatc tacgaacacg
     7261 cgccggtccg cagcgctgac cgtttttccg gcatcgggaa catcctgcca atttttcggc
     7321 cagaacattg catcgccgta acgcggtccg ggcgtggtgc aaatatcgac gtacatttgt
     7381 tctgatttcg tgaacacgct ggggtcgata atttgcgctg cggtcatttc atccgccatc
     7441 cggaaaaatt gcttgcttgg attttgccgc aacggttcca cggcatggat tttgaaaaga
     7501 tttgaaatcg ggctgtccgc gcgggcggcg atttcatcaa tcatcctgcg gcccggggcg
     7561 atctcatcac cttcgtgaat ttcctcggcg atatcaacgc tgatgatgct gcatttcggc
     7621 cacgccgcgc gcaaaacttt tcgcgcggat tctggatcga aaaacatatt gaatgcattt
     7681 gtgaacacgt gaaaacccgt gcccatgatc acgatttcgc gcaccaaggg tacgatgtcc
     7741 ggcgcgagtg caattgcgag cgcaatattt gttaacgggc cgcccgcata gacagtcaca
     7801 tttcctggat tttcgcgcac acttttggcg atgaaacgcg cggcatgctc ttgctgcggt
     7861 ttcaaattgg ccagccggtc atagggtggc gcgagcggct tgatttcatc tggtccgccg
     7921 ctcgtgcgat tgaatgcgcc catccatgga tccatccggt ggccgccata tagttgctgg
     7981 cggagctgcc attcttccct ggtattgagc atggggaact ccgtgcccgg atacacagga
     8041 atttccgtgc ggcccatcat ctccagtaat cgcagcgtgt aggccatttc ctgtcgcatc
     8101 catgcgtcgc ccgtcacaag cgtaatcccg agaagatcga tggtttcagc ctgcaacatc
     8161 atcaatatgc ccagaatatc cgtgccaaaa gggccgtcat tatcctggtc gtaaatcact
     8221 tttcgctttt cggtgggctg cgatgcttgc gccagacttg gcgcaatttg cgctgaggca
     8281 attgctgcac ccacggtttt gataaattcg cggcgcgttg tactcatcgg gtctcgcttc
     8341 ttgtagcgac aggcttcagc ctctcggctt ttgacttttt ttaatctact cagatctaaa
     8401 cccgacaggc tgaagcctat cgctacctag accgattgat cggtcttgtc atcaaatccg
     8461 tgaacaattc gcgaaagcgt ttcttatcaa ttccatttaa tacctgcacg cggcctgctt
     8521 tgagcgaagg cccgttctgg ccagcgagct caccgttttg attcttccaa ttggcactcc
     8581 agaaggatgt gtcgccataa tggccatcgt tgtgtgtcac gacgtcgata aacatgtcgt
     8641 cgtatccagc gaaaatgctg gggtcgatga cgtgcgcggc catcatttcg tcgggcatgc
     8701 ggaaagagcg gcgctgcgga ttggcgcgct gcggtttttg ttcgtactcg cgaaatagat
     8761 cggcaatggg cgattgcgcg cgatcgacaa tcgcgtcaac cttgccgtca tcctgcatat
     8821 gcacttgctc agcgagatcc gtggtgacta aagtgaattt cggccacgca gcgcgaaaac
     8881 cgatcttggc tgcttccgcg tcaaaataaa cattcacgga gcttgtggaa tgatgcaggc
     8941 ctccccccat gaaaatgact tccttggtga tgggcacgat gtccggcgcg agcattgcag
     9001 ccagtgcgag gttcgtcatg gggccgccgc aatacattgt cacttcgttc ggatttgcgc
     9061 gaatagtgcg gatgataaaa tccgcggcag gttcaggctg cgctttaatc tgggcgaagc
     9121 ggttgtaggg aagcgggagc ggcgcgagct catcgcggcc cggtgcgtct ttcgcaaagc
     9181 agcccaagta tccttcgttg ccgagagtgc gcgacccgaa catctggtag cgcatctgca
     9241 cttcaggctt ggagttgagc aaaggttcct gagcgcccat aaacactggg atctctgtgc
     9301 ggcccatgag ctcaagcaaa cgcagcgcat aagcggtttc ctgcttggcc cacatgtcgc
     9361 agcttaccgt gcaaattccc agcaggtcga tgttgtccgc ctgaaggagc atgagcgttg
     9421 cgaccgtgtc tgtggaaagc gggccgcgat ggtcctggtc gtaaattact tttttcttgc
     9481 cgccggatgg cgaagaagtt tgcggcgcag gagaaaacat ggaactgtgc gcgagaaaat
     9541 tatgcggagc atgcattggt ttcctcattt gaacgaaagt aaggaataat actaaattca
     9601 aaattatgaa ccgtggcttg gcattgcgat tctacatggc aagtggcggg gggcacggat
     9661 gaatcatttg aattcaaacg atctggaatt gaataaaaaa aatcgcaccc ttgtggaaaa
     9721 cgcaagggcg cggcacccac tcataaatta agccaaatga agcgtgactt tgggagctaa
     9781 aacttccgac tatgctgatc gccccagcgc tcgatgacca cttttttatc cgtatagaac
     9841 tcgattgcgt cacgaccttg ggcgtgcatg tcaccgaaga agctgccttt ccatccggtg
     9901 aaaggaaaat aggccatggg ggcggccacg ccgatgttga tgccaatgtt gcctgcgggc
     9961 gcttcatagc ggaagcggcg cgctgccgat ccgctggaag tgaacaggga ggcctggttg
    10021 ccgaatgggc tgcgttcaag gaaggcaatg gcttcatcca tgtcgcgggc atgcaccagg
    10081 ctcaacacgg ggccaaagat ttccgtgtct gtcagttcgc tggttgaggg tacgccatcc
    10141 aaaattgtgg gcttcaggaa atttccgcct tcgtgctttg gaattttggc gttgcggccg
    10201 tcgagaatta cttttgcccc ttctttttcg ccgactccaa tcagcgattc gattcgcgat
    10261 ttgctttgcg aagtgatgac cgggcccatt tgcacgcctt cgtccaggcc atcgccaact
    10321 ttcaatgacg cggcggcttc cgcgatcgat tcgcggaatg ttttctgcgc gtcgccgatg
    10381 gtgaccgcaa cagaaacagc gaggcagcgc tgcccggcgc aaccgaacgc gctgtcgtta
    10441 ataatctgcg tggccatttt catttctgcg tcaggcaaaa cgatgacatg gttttttgcg
    10501 ccgccctggc actgcgcacg cttgccgttg gcagcggcgc gcgaatacac gtatttcgcg
    10561 attggcgttg accccacgaa actgacggcg cggactttcg gatgatcaat caacgcatcg
    10621 accacggttt ttccgccatt gatcagagtg acgacgccct taggcaatcc aacttgttcg
    10681 agcaattcaa acacgcgaac catggtgagc ggaacgcgct ccgacggttt caaaataaaa
    10741 gtattgccgg tggcaattgc gtagggcaag aaccagaagg gaatcatgcc cgggaaattg
    10801 aaaggcgtaa ttgccgcgac gacgccgagc ggctggcgaa tcatcatttc gtcgatgccg
    10861 cgggcgacgt cttccaggtt atagccctgc atcatcgtgg ggatgccgca cgcgacttcc
    10921 acgttttcga tggccctgcg cagttcggct tttccttcga caaatgtttt gccgttttcg
    10981 cgcgcgatga ttcggccgag atcatctacg tgctcttcga gcaacatttt cagcttgaac
    11041 agaggctgga tgcgatcttc cgccggcgta cggcgccact cacggaacgc aactgccgct
    11101 gtttctacgg ctgcatcaac ctcggcagcg gtcgaaagcg gcgtagaggc gatggcttcg
    11161 cccgtggccg gattcattac atcgcgccat tccgctgatt tcgattcagc ccaattgcca
    11221 ttgatgaaat tcaaaacttt ggtgctcgtt gcagtggtcc cagtcataac tttcctcaat
    11281 tcaggtgcta tcggatgaac ccaacaataa aattccaagc ggaattattg taaccgcgcg
    11341 atttctaaaa taaaacctgc aaaactaggc actggttttg cttgcggaat tcagaaatct
    11401 ttcgatccgg tccagcacct gcatggcggt atagcactgg gcgacgctgg cattcggttc
    11461 gcgcttcccc tgaatcgcgg caaaaaattc gcgatcctgc aactcgatcc cattcatcga
    11521 gacatccacg ttggacacat caattttatt ttcgtcgccg tccaggagat cgtcatagcg
    11581 ggcaatgtac gtgccgttgt cgcagatgta ccggaaaaat gtgcccagcg gccctttgtt
    11641 gttgaaggaa agcgacagcg tgcaaatcgc gccggagggc gttttcataa cgatgcccat
    11701 gtccatggcg atttttaatt ccgggtgaat cggtccctgc acgccgtagc actcggaaac
    11761 gagttcgtcc gtttgatact ggaagagatc gactgtgtga caggcgtgat gccataggag
    11821 atggtcggtc cagctccgag gctttcccgc ggcattcata ttcgtgcgcc ggaaaaaata
    11881 cgtctgcaca ttcatctgct ggattttcaa ttcgcccgct gtgattttct tgtgaacata
    11941 ttgatggctg ggattgaaac gtcgcgtgtg tccgcccatg caaatgacgc cagtttcttt
    12001 ctgaactttt accaggcgtt cggaatccgc gagagtatcc gcgatcggaa tttcaatctg
    12061 cacgtgcttg cctgcgcgca tgcactgctc gccctgcttg gcatgcaatt gggtcggtgt
    12121 cgcgaggatc atcgcttcga ggccaggctg cttcaggctt tcgccgagat cgttcgtcca
    12181 atgggggatc ttgaattttt tggcaatttc ttccgtggcg gcctgatttc cgccagttag
    12241 agtgatgact tcaatgccag gaattttggc tatgccctcg agatgctttt gcccgaatgc
    12301 gccctgaccc gctacgccaa ctttcatctc gtttctccct gagaatgaat ttttgtgtca
    12361 ctaccttatc ataatcatgg tttcgcggac ctgtggaggg tgcagggagg tagtgttcct
    12421 tgttccaacg gataattgcg ttctctggcc agaaatacac cagacagaga atcctctacg
    12481 aaaacgtaat ggactttcgt cattgccaat gtgacgcgcg cccgtcattg cgagtcggaa
    12541 gctgtattgg aactgcaatt caattcgatg cacaacctgc ccccggctga aaatcttttc
    12601 cgggagcctt attctttggg cgcggctgga atgcacactt tgtcctggtg caagaaatcg
    12661 acatcgtgaa tctcgctcgt gactctcact gattggagcc ctgccttttt ttctgtttta
    12721 ggctggaagg aaagtaaaaa ttgattggga agctgctcgt ttaatttctt gagataagga
    12781 ctgaaggata cgggagcttg ggggcccatc aagtaatagc tttctccgcc ggtcgcttca
    12841 gcaagctcgg aaagataatt ttcgccccag taagttctgt aataactgtg acccatgtga
    12901 ccggcgcttg gggtataaat ggagtacacc acgacgcccg cacactgggc atcctggatg
    12961 gccgcatcga cataaggatt gtctatattc cccatgtaaa cattatcgat cccggacgta
    13021 atcatcagga cttcacgtcg aatagccggc gagttggaat gccatccctt gatgaatgtg
    13081 gaaagtgaaa gatacgggct tccctccgcg ccaaaatatc cttgtgtcag ccggatcgat
    13141 ttcgtcgtag cggcgtgctc tgaagtgaaa tcgcttgcca ggttcaccgt accgttctgc
    13201 atgtagccta cagcaattaa agtggaggga ggctgctgcg agatgaagct tcggatatca
    13261 tttagctgag cgcccaggct gaaccctgcg ctttcatcga tcaagattgc cagctgaagt
    13321 ccggcttttt ctcctttggc tgggatccag ccggtgacag ggagccgatc atgcccttga
    13381 tagaccatta tgtcttgctg agtaatcata gggatctggt tgccatgcct tggttccacg
    13441 ctgacgatca tttgaaccga gtccccagca gcggtcgccg tgtcctgggc tcgcgtggag
    13501 cacaaaataa aaaataaagt taaaccgaga gtaattactg caagaaactg ctttggcggg
    13561 atgtgaagcg gattgttgat cgagaaatgt ttagcttcac cagaaaattt gtagaaagct
    13621 ctccatctct ccgagagcct gagcggtagg ggtaacatgg ccattcctcc ttaattgaag
    13681 tcgatcgaaa atgttgggta atttctgagt tcatttgatc gagtttagga aagcaagctc
    13741 ctcacgagca atgatcgcaa gcatgagggc tcgtgcggcg agttactcgg tacggtaggc
    13801 cctatttagt tggttgtttg gagtagcgat ttggttggct gacattttaa agcatggatt
    13861 tccagataga acaaatagag attcttgtgc cggcaagcgt ttagtgcaac aattcccgcc
    13921 gcccactggc gcgtgtcgcc agaactaaat atcaaaccga gaattggaga gttgagacat
    13981 gagacgaatt tttctgtcct tcgccgcggt tgccattctc ttaggcgttt ccgcgcaagc
    14041 atttgctcaa acggaagtaa cactcatcgc gccgggcgga attcgcgctc ccatcgagaa
    14101 actcattcct ggatttgaaa agaagactgg ttacaaggtt aatccgacat tcggttccgg
    14161 cgggggaacg aagaagcaag tcattagcgg cgatgtgttc gatgtgccca ttgtacagcc
    14221 accgctcgcg gaagtaaccg cgtcgggcaa cgtggtggaa agcacagcga caccgctcgc
    14281 gtcggtggct gtgggggtcg ccgtgaaaaa gggtgcgccc aaacccgata tttcttcgag
    14341 cgaagccgtg aagaagatgc tcctttcggc caagtccatc gtgtgccctg acgcaacgac
    14401 gggagcggcg gcaggtgtga gcattaacga tatgctgaag aagctgggca tcgacgagca
    14461 agttgagcca aagctcaaac gcgttcgcgg aacaggagca gggggcgccg gcggcggcac
    14521 gatggtttcc ggcatgatcg caaatggcga ggcggaactc ggcatgacct ttgtgagcga
    14581 aatgaccgat cccggaatcg atgtcgtggg agtgctgccg aaggaagttt cgccatggac
    14641 gccactagtt ggctacatct ccactcattc gaaaaacccg gaagctgcca aggctctgct
    14701 taagtatctc tcatcgcccg aggctttagc ggcctacaaa gcggcgggaa tgcagcctgg
    14761 aaagtaattc aggaaagttt tgggatcggc tggcggggca agtcaggact tgcccctaac
    14821 cctctagatc tgaattcaag aatgcgttag gcctgttttg agcatacaga tggcacacta
    14881 aaagaaaaac cgccgcagcc aggtagctga gactgaattc cggcgccacc caccagccga
    14941 gatgattttc gataccttgc gaagttagct gataggcatg gattaccccg aagcaagaca
    15001 gcacggcggc gacaagcatc catgccgctg actgcaggaa acgacgctcg atgatccaca
    15061 ccagcgctgc tgcccagatc atggagacga gcagcgcgcc ttgtccaaga gcaatcatgc
    15121 cgtagattgc aagttcgccg ccaaagcgcg gagcaaccgt cagcatgtcg gctcctgctt
    15181 tgcgtatcgc caaatcgacg atctgggtta cccactgcgc cagcattggc acgaggccca
    15241 gcgcaacagc cacggaatgc gctacgggga cttcctgaaa cgcctgcccg accatcgcca
    15301 gtccaaacca cacgactacg aaggccacaa cttccagcgg aacgaatcgc agcacggcag
    15361 gcactaaccc cgtgaggcaa agcgctgcgc tgacaacacc gcccaaaatc gaatacccga
    15421 ttcttgcacc gttcgcttta tgcgcgttgt ggcctatata gagtgaagta gggaacgcgc
    15481 tgcccagtgc cgcgcccacg atcgtggcga ggccattgac caacagagat ggatttgtcg
    15541 gataatcatc gccggctacc cttacgcttt cgagaatttg cagcgaaacg attgtgtcca
    15601 gtaaactcag cgggagaaag atcgaagcgt agcgccagcc ttcagccttg acgataatct
    15661 ggtagaggtt gacgggccgc ggcaaataaa ttcccggagg aagcaatggc ggctgtggtg
    15721 gcaagtgata gacgtgaaat gctcgtagaa tcacaacaat gattccgccg acaaccatgg
    15781 ctacaagtcc cgagggaatg cgccagggca gcttcatctt tgatgcgtag gtggccagca
    15841 gaatcactgc aggaagtagc gccaccgccg gtatctcaaa aattccgaaa ataaatccca
    15901 ggcaaagaaa agccattgcg agccccgcca acgggcaaag caatgctgcg aggggtgtcg
    15961 tgcggcgcat ccaatcgatg caaaatgcgc tgaccgattg cacgatgccg ctaacgaggc
    16021 tggcaaaaat tcccatctgc caggcgagat tcgcatcatg cgtgcgctcg taaacgggca
    16081 gcataatcag ggagatgtaa gcaaaaatgg tgggagtgtt gatgccgaac ggaattgcgg
    16141 tcacatcgtt gcggctagaa gatcgcgcca aacggtgagc ctgccaggca taaaataaat
    16201 tgccgaacaa gatagaaaat gccacggccg gcaagatgcg accggtgacc aattgttgag
    16261 ggatgccgca aagaggagtg agactggcga taagaagcaa gtcaggaaag ccgctaaaaa
    16321 aaagcccaaa aaaaccgtcg aggtcgcccg cgacaaacca gcgcggtccc gtcgtttcga
    16381 ttggttgggt ggcgctagcg atgagcgagt cctcgcatga ttctcatttt ggttgaaatc
    16441 acgaagttcc gtattgcagg cccaattgct ttagccagcg gcgatacgcg atggcaattt
    16501 gatcggatcg aagatgcaat tcggattgca gatcgagagg cagcaattca gggcgctgcg
    16561 agttaacgat gcaaatgtcc tgcgctgtga ctttatcctg gaattctcgc aattcttttt
    16621 ccggaacttc atgcgcgtaa tccatggcaa tcaccaccca tgcaagggtg ttttcatcgt
    16681 cgatcggagt gaccgtacca aggatggcga agtgctgcgt gggatgcgtt ttggttaagc
    16741 gagttgtaaa ggggcgctcg acccagtaag tgtagtgaac gttagcgcca cgaccagttc
    16801 catcgggatc aggctgccaa agtggaatgt cccgcgccac aattccttcc gcagtggttg
    16861 tgaccgcgta ttcgccaatc tccgctttcg acgggtctcc tagcatgccc gcgtgagcaa
    16921 tgggcaggtg cgcgaggtcc agaatgtttt cgatgatgcg tggaccttgt gcgtggaatg
    16981 cataagggcc tgtggatatg agacgaaaac ctggggtttc gccttcagga aacgatggga
    17041 tttcgcccac tggttttccc aggcacaccc acacgaggcc gtacttttcg cgaacctcat
    17101 agcaatccac atgtgcgcgt ggaggcggtg cctgatctgg atgtgcgggg atgcgcacac
    17161 atgcgccggt ggagtcgtac tcccagccat gatacgggca gaccacgcac tcgctttttt
    17221 cggactttga aattctgccg agggaaagtt ttgctccgcg gtgaatgcat aggtctttcc
    17281 acgcgtggac gccgcttgtg gatcgccaca ggaccaggtc ccggcccagc gcttgtacag
    17341 gttgcaattt attttccggt agagaacctg tccaggcgac agcgaaccat tcgttcagga
    17401 ggagtgggtc gttgagtccg acgtcttgcg gcataaaagt ttagcacctg aatttcggag
    17461 gtatcgtatc gcatcttgtg gagcgattga aatcatggtt atgaatagga agtatttgcc
    17521 catccataat tagggcatta ggattcttgt ggcgccggcg ggacgccgcc acgaaactgt
    17581 cagccacaaa gaagagtaca aggattgctg ttttgccagc tagaccgcat ttatgaaaag
    17641 ctggccgcgg cggactacgg ccttgctggt tagcattttt tgtccggtcc gttttatgcc
    17701 gttgctatct tcgaaatcaa atttgccttc gcgcaattcg tagatgctga cgtcagcttc
    17761 gcttcctggg cgcagagtgc cgatttgcgc gccgaaatcg aatacgcgcg tggggtttat
    17821 cgtgaccatc gcgatggcct tatccaaagg caaaccgatg gacaaaaatt tggaaaccat
    17881 tgtaggtagg tcgaacacgc gccgctcgat ggtaacaatc gtcaggtccg tgctgatggt
    17941 ttcgggaaga aagccctgcg ccatgcactt ttcggctacg tcgaagctga aatgcgtttg
    18001 cccttgtgct ggatcgaaaa taatcccacg ctcgcgcgcg tctcgggctt ccggaataat
    18061 ttttccgttg gcgtccagaa tgctgtgcgc acggttgttg tagcagtggg tgaagacatc
    18121 gcccttacgc agcaatttta aaatatccgg cagcggcgaa aaagagtctt cgatgtgcgc
    18181 catgagcggc atgtgcgcaa tttccgcagc ttgcagcgcg cgcttcagac actccaggtc
    18241 attcgatccg gtgatgggct tgctcaactg aatcttaatt cccgccgtga caggtttatt
    18301 gtccgtcgcc atctttgcgg tcgcctgcgg gttgagccat gaaagattcg agaggccgcc
    18361 ttcgtacatg atggtgccga tcgggctgat gtgcacaaga gcaacaatgc gagtggtcga
    18421 agtgttgatg atgtattttt tgaaattggg aaacgttgca gtgcctgccg aacctgcgtc
    18481 aagcattgtg gtgacgccgc ggttaaggca gtagcgatcg gcgttcatcc caatgccaac
    18541 accgtcatag caatgcgcat gcatgtcaat gaaacccgga gtgacgaggc ggtctttcgc
    18601 cgacaccgtt ttcagcgcac gatctttcgc aatatttttt gagacttcga gaatttttcc
    18661 gtccttgatg gcgacgtcca tcaagccatt cagattttgc gatgggtcga taaccgtgcc
    18721 gccctcgatc agcaagtcga atttgccatt cgcattttgc ggcgcggcag gcgggccggc
    18781 agcgccggac cacagcccgc gcggtgcgag cattcctgcg cccccagcca agactaattt
    18841 cgcgaaatca cggcgattcg gcatgtttat cgctcctcag ccatttcgat caggatgcga
    18901 cgcagtctag cacattcaag cgaagttact tcgttccagc aaggcattaa aattaatttt
    18961 ctggtaaatt cgcagctgat ttaacgggct gagaaatact caaataagat gaatattcac
    19021 caatcgacga cgagttatga aaagtgggtg tcttcctgca ttccgttgga ccggcacgcg
    19081 attgtcttca aacataagca aatggcaacc gggctctttc cattcttgcg cgcgacgttt
    19141 tatcgctgga tgcaaatatg gcgggaagaa gtgggagatg ccgcgattgc gccatcgtta
    19201 ttggccgttg gcgatttaca tcttgagaat tttggaacgt ggcgggacgc agagggccgc
    19261 ttgatttggg gcatcaatga ttttgacgaa acgtatcgaa tgccctatac gatggatctc
    19321 gtgcgtcttg ccaccagcgc ccacgccgct gcggtagagg aacacattgc tgtccgtccg
    19381 cgcgatgcga gcgaccaaat tctggaaggt taccgagacg cgctgaaaga gggcgggcgg
    19441 ccattcgtgc tcgcggaaaa tcatcgatgg ctgcgaaaaa tggcgctgag ccggttgcgc
    19501 gatgcgacag tgttctggca aaaattggaa cggatgcaaa aatctccgca ccgattacct
    19561 cggcacgtgg agtcgcatct tcgcaaactc ctgccggacc gttccatgcc gtgtgagaag
    19621 aagcggcgac tggccggttt ggggagcttg ggccacacgc gtgtcgtgct gctcggtaaa
    19681 tggcatgact gctatgtggc tcgcgaggcg aagcagttga cgccgtcggc ctgcgtttgg
    19741 gcgcgcggaa aaaacgcgga ggggcacttg gcgcgctatg gtgaaattct cgatcatgcc
    19801 gttcgcgtgc ccgatccatt cgttcatcct tatgatggtt ggttattgcg gcggcttgcg
    19861 ccggattgtt cgcgcatcga gcttgagtcg ttgcccgaga gccgcgacga ggaccgcctg
    19921 ctttattcca tgggctggga aaccgccaac attcatttcg gcgcgccgcg tgcaattcgc
    19981 gcgatcaagc gcgatctggc caagcgaccg gcgaattggc tgcacaaggc cgcgaaagcc
    20041 atgctccatg ccaccaaacg cgattggtgc gattgggttg cgtagcgtag gcgtcccgcc
    20101 ggcgctacgc aaccacaaag catggtgagc caatttagag cctcatttgc gttaaattgt
    20161 gatgagaagg gttacgcctc ttttcgcttc taccgcttct gaaaattgaa gtagaatgtt
    20221 cggccagaag atgtctccgc ctgggtgccg agcattttct ttctggaatg aaattgccgc
    20281 gacgcatttg atttttcgaa aaaaaccgca aaaccattat ttcctaccaa gctgtaggcc
    20341 cgttaatgta caaattttat tttctggtat gctcagtttt ctgatgattt gaaagtgcgt
    20401 tttgtatagg agcgcaatca cgaaagatga tttgcaagca ccggctttag aattgaaaga
    20461 aattccagga aaaaattaac caatggcgat aaaaaaactt ctggccttga atcgtggaga
    20521 aattgcgatt cgcatattcc gcgcagcgaa cgagttaaaa atccggactg cggccgttta
    20581 ttccgatgag gatcgcctca gcctgcaccg gttcaaggcg gacgaagcat atctgatcgg
    20641 tgaagggaag ggcccggtcc aggcgtatct cgacgtcgat ggaattgtgg cgctcgcaaa
    20701 agaacgaggc gtcgacgcaa ttcaccctgg ctacggtttc ctttcggaga atcccgcgct
    20761 gccacgagcg tgcgctgcgg caggaattat ttttgttggg ccaagcgcgg atctgctcga
    20821 tttgctcggc gataaaacag ctgcgcggcg gctggctcag caagctggat tgcctgtcgt
    20881 gccggggacg gaagagccgg ttacgcaaaa ggatgacgcg caaagcatcg cggacaagat
    20941 tggctatcca cttattgtaa aagctgcgtt cggcggcggt gggcgcggga tgcgcgttgt
    21001 gcagagcgcg cagttattac ttcctctttt gcaggaagcg cgtgctgagg cgggagcttc
    21061 attcggcaat gacgctgtgt ttcttgaacg ctatattcgc cgcgcgcgcc atattgaagt
    21121 acaaattctg gccgacacgc acgggaatgt tatccactta catgagcgcg actgttccgt
    21181 gcagcgcaga aatcaaaaag tggtggaggt tgcgcctgcc gtaaatctcg ccccgaaaat
    21241 tcgcgaaggt ttggcgaaag cggcggttgc acttgcacgc gaagcaaaat actacagcgc
    21301 ggcgactgtc gaatttttgg tcgatgcgga tataggcgaa tattatttca ttgaagtaaa
    21361 tccccgcgta caggtcgagc acaccgtcac tgaaatggtg acgggcatcg acatcgtgca
    21421 atcgcaaatt caaatcgcgc agggctacga tttgcatggt ccgcaaatga atctgccgcg
    21481 gcagaaagat atttcgatcc acggttacgc gctgcaatgc cgcgtgacga ctgaagatcc
    21541 cgccaatgga ttccttccag attacggcaa aattcaaaca tatcgttcgc ccgcgggctt
    21601 tggaattcgc ctcgatggcg cgtccgccta tggcggcgcc gtcatcactc cttactatga
    21661 ttccttgctg gtgaaagtga ccgcatgggg ccgcgaattt ccgcaggcct gccgccgaat
    21721 ggatcgctcc ctcaacgaat tccgaattcg cggggtaaag accaacattc cgtttcttgt
    21781 aaacgtggtg aatcacgagc attttcaggc gggtggcgta acaacttctt ttcttgcgga
    21841 aactcccgag ctattccatt ttgcgcaaca gaaagatcgc gcgacacgca tgctgcgcta
    21901 tctcggcgaa gtgattgtaa atggaaatcc ggaagtcgcc gacaagccca ggccgcagtt
    21961 gattcgccct gcaccgacac ccgcccacat tcacggtgaa ccgccgcccg gcacgcgtca
    22021 acttctcgaa aagttcggtc cggaaaaatt cgcggagtgg acgaaatcgc aaaaacagct
    22081 attgattaca gacaccacat ttcgtgacgc gcatcagtca ttgatggcta ctcgcgtccg
    22141 cacgtacgat ttgctcgcca tatcgaattt cgttgcgcac aaactacaca atctttacag
    22201 cctggaaatg tggggcggcg cgacattcga cgtttcgatg cgctttcttc tcgaagatcc
    22261 ctggatgcga ctcgaactgc tgcgggaagc tattcccaat atttgcttcc aaatgttatt
    22321 taggtcgtcc aatggcgtgg gctactcagc ctatccggac aacgcggtag aagctttcat
    22381 tctggaatcc gcaaggcagg gcatcgatat tttccgtgtg tttgacgcct taaattggct
    22441 gccaaacatg aagctttcga tcgaaacaat tcgcaagacg gacaaagtat gcgaagccgc
    22501 catctgctac acaggcgaca ttctcgatcc gaagcgcgac aaatatccgc tgcagtatta
    22561 cgttcgcctg gccaaagaac tggaagccat gggcgcgcac atcctatgca ttaaggacat
    22621 gggcggcctg gtaaagccct acgcggccga gaagctattt tctgttttgc gcaacgaagt
    22681 ggcgattccg attcattttc atacgcatga caccagcggc gtcaacgccg catccatcct
    22741 gaaagcagcg gaagccggtg tgaacgcggt cgacgcggca atcgcatcga tgagcggcac
    22801 cacgagccag ccgaatttaa attccattgt tgccgcgctc aagcacacgg agcgcgatac
    22861 gcggctcgat ctcgaagcgc tgaacgcttg cgcggattat tgggaaacag tacgaacgtg
    22921 gtaccgcccg ttcgacaatg cgcctccggc tggtacggcg gaagtgtaca ttcacgaaat
    22981 gcccggcgga caatacacga atttgcgcga gcaggctgaa tccatgggac tcggtgcgcg
    23041 ctggcaggaa atcgcacgca cctatgcgga attaaatttc gcgtttggcg acatcgtgaa
    23101 ggtcacaccg tcgagcaaag tagtaggcga ttgcgcgatt ttcctggtca gccatgacat
    23161 gacagtgaag gacctggagc ggcttggccc ggatcataat ctgacgctgc cgaactccgt
    23221 cgtagaaatg tttgctggag cattgggcga gcccgaaggt ggatggccgc cgaaactaca
    23281 atcggtgatc ttgcgtggag cgaagccggc gaccgggagg ccgggcgaac acctcgagcc
    23341 cgtcgatttg gaatggacga gggcgggggt tgagaaaaaa gctgaacaga aaatctccga
    23401 tacggacctg atgagttaat tgatgtatcc ggacgtattc ttgaaatttg cggcagcgcg
    23461 cacggcgtat ggaaacctgg aagcgttgcc tacagtgcaa ttcttctacg gattgcagca
    23521 gggccaggaa attaccgtgg agctggaagc cggtaaaata ctggttatca aattgctatc
    23581 agtaagtgac gcgagacctg acggtacgcg aaccatcttt tttgaactga acggccagcc
    23641 gcgcgaagtc actgttcgcg accgcgcact gcaagctacc gtgcaaacca ggccgaaggc
    23701 cgacccaagc gtgcccggcc aggtcgggtc gccgattccg ggagcgattt cgagcatcgt
    23761 ggtggaggtc ggggaacaag tgaaaaaggg agaccgcctg ctggtgttgg aagcgatgaa
    23821 aatgcaatcc acggtttatg cgccagtggg cggcaaagtt gctaaaaaac tagcaaacgt
    23881 gggtgacaag gttgaggcta aagacctgct acttgtgctg gaataaatta taatcaaaaa
    23941 taattctatt ctcgttgaga aaatccgcgc atagtaattt cgatgcgtaa acctcaaaaa
    24001 aaagcatctc tccgcataaa acgtttgcag cgtacggatt tgcccaccga tacgatggat
    24061 cttgccgctt atttaattgg gaagacgctg gtgcacgatt tgcctcgcgt tcgcctctcc
    24121 gggcgcatcg ttgaaactga ggcgtatcca gtaggagata tggcggccca cgcatttcgc
    24181 ggagagaccc caagtatccg ctcgctattt ctgccgcatg gccacgcgta tgtctatttc
    24241 acgtatggtt cctgcttcat gatgaatgtg tcgagcgaag aagtcggaac cggcgcggga
    24301 gttctggtcc gcgcgctcga gccgcttgag ggaattaaat ttatggagcg gcgccgcgac
    24361 accattcgtt tacatgatct cacgcgcggc ccgggccgcc tcgcccaagc gatggatata
    24421 aataagcgcc atgacggctt gaatttatgc gcaggcggaa aattgtggct tggtgatgct
    24481 gtccggcctg caggccccat cggcatcagc atccgaatag ggattacgcg cgatgttcac
    24541 cgcccctgcc gtttttatga gcgcggaaat ccttttgtga gtggcttaaa acgtatgcgc
    24601 gagtgagcat ctaaattggc cttccaatca agatgcggga gtacttgtgg cgccggcgtc
    24661 tcgccggcgg tttttttccc cgcgtaacta acatcaaaaa ccgcccggat tcaacgaacg
    24721 cttgccatgt gtggtaataa taaatcaggc ccaaaaggag catacaatgg caactcagga
    24781 agcatgtccg catgtcaagg aaatcaaaaa cgtaaaaccg agtgcgaatg gctgtgaaga
    24841 atgcctgaag acgggcgaca cgtgggtgca tttgcggatg tgccttatct gcggccacgt
    24901 tggatgctgc gattcctcga agaacaagca tgccaccaaa cattttcaca ggaccaaaca
    24961 tccgatcatt caatcgatcg agccgggtga aaattggcgc tggtgttacg tggatgaaat
    25021 cgaagtcgat tgagcgatct cgcgccgggg caaattagct gagggaggaa atcatgtcag
    25081 ctgcgaaaac agtcttggat tcctcggaag cgtggcctgg tttgccgtta tctgcctggg
    25141 ccgagacgta tgcaacactg catatgtgga cgcaaattgt ggggaagatc cgtctggcaa
    25201 tgtccccgca tatgaatcat tggtgggaat gcccgctgta cgtcaatgcc cgcggcttga
    25261 ccacttctcc gattccatac gccaagggcg catttgaaat tcagttcgat tttatcgatc
    25321 ataagttaga cgtaacatca tccagcggcg aaacaaaatc catgcgtttg cagccgtgca
    25381 cagttgcgga ttttcacact gaaatattcg tgctgctcaa gtccctcggc atcgaagcaa
    25441 aaatctggaa tatgccggtg gagattccca acccgattcg cttcgatcag gaccgaatcc
    25501 ataacgcgta cgatcctcaa gcggttaacg cgttctatcg aatccttgtc aacgtcgact
    25561 gtatcttcaa ggaatttcgc tctcgctttt tgggcaaagt gagtccggtc cattttttct
    25621 ggggcagttt cgatcttgcg gttacgcggt tttccgggcg tcgcgcgccg gaccgccctg
    25681 cagcggacaa aataactcgc gacgcgtatt cgcatgaagt gagcagtgtt ggtttttggc
    25741 cgggcggcgg tgacatcaac ggagcggcat tttattccta tgcggcaccg caaccggagg
    25801 gatttggaga gcggcctgtt cgccctacgg ccgcttatta ccataaacag atgggagagt
    25861 ttttgttgat gtacgacgat gtgcgcgctt cggattctcc gcggagcgcc ctcctcgact
    25921 tttgccagag cacgtacgat gcagccgccg ttctcggaaa ttgggatcgt gccaacctgg
    25981 agaagccggt ggtcaagtag cgcacacgcg gaagtaagat atggcaaatt tccgcggccg
    26041 aaatgcttga tttatgtatt ctcatcgctg cccccctcca acagtccagc acagatttag
    26101 tcttcgattt caggcgcaat ttaatgtttc gatttctcgc aaaagtcgtg ttcgagttta
    26161 taaatgtttg accatcggac tacgcaccgc tgccatccag gtatttgcgt tatgcttggg
    26221 gaataacaac ttggcaggtg ccatgccgcg cgcttgcggg gcagggtatg ctggcagtta
    26281 aagtaaccag agatgcagag ttgaaatcat aatggcgcac ttcattcgga ttatcgcttt
    26341 cgtaacatac gcatgcgttt gcgcattgcc gatgctcgca cagcaggatc gtggcgaact
    26401 tcaaatcgaa gttcatgatc cgcagggaac cttgctgagt gcctccggag aattagtcag
    26461 cgaaggaaat caattccatc tcacttttac agtggatgac gaaggccgtt ttatcgcgcg
    26521 tggcctcgcg ttcggtattt atcgagtcag cgtgtctcac tccggatttg tctccgctgt
    26581 tcaattggtc gagatccgca caattgtgcc aaagcatttg agtttgaccc tgggcctgaa
    26641 acccgtggaa acacaagtgc aggtcagcga gacggagacg ctggtcgatc ccacacgtac
    26701 gaatacggtg tacacgattg gatcgcaaac aattgacgag cagttgtcgt ctcggccggg
    26761 acgtggcgtt ctggatgtcg ttaacgcgca gccagggtgg gtgtacgaag ccaatggggt
    26821 tttgcacccg cgtgggtcgg agtatgacgt tcagtttgtt gttgatggct tgccgatgac
    26881 cgagaatcgc tcgcccggtt ttgcgccgcc aatcgaatcg agcgatgtcg aatccatgcg
    26941 cgtgatgacc gcggggttcc cagcggagta tggacgaaaa ctcggcgggg tggtggaaat
    27001 tacaccgcca aaaaatactc ctgccggctt ccatggagag tttgaggcgg agggcggaag
    27061 cttcgatacg ggctcggcga gcggcgctct tttttattcc attggaggaa accgattttc
    27121 cattagcggt gatgcatttc attccgatcg atatctcgac ccaacggttc tccaaaacta
    27181 cacaaacatc ggcaattctg gaggattttc aacgtcatac gagcgcgatt tttcaaatgg
    27241 cgatcgtctt tttttcagtt tggggcaaca cgcggttcgc tatgaggttc cgaatgaact
    27301 cgtgcagcaa gtagcgcagc agcgccagaa ctctgagcaa aaagaaactt caggccaggg
    27361 gcgctatacg catgcttttt ccacggacat tttctttagc atcgcgggaa gtattcgcga
    27421 cgcatccgct tttctgaact ccaacgcaca atcgacgccc atcgtcgttt cccaaaatcg
    27481 cggttatcgt gaaggttata ttcgcgcgga cctcgcgggc catcacggcc gccatgattg
    27541 gaagtttgga gcggatggaa tcttcagtcc ggtgaatgaa aatttacagt atcggattac
    27601 ggacctttca caattcgatc ctggtactgc gctgagtttt caattttccg accggcgatg
    27661 ggatatagaa ccctcggcgt ttgtgcagga ccaaattcga tttggaaaat ggaacgtgag
    27721 cgccggcttg cggtacgacc attacggttt tgcagttagc aaatcagcgt ggagcccgcg
    27781 gcttggcgtc tcgcgattta tttcatcctt ggacctcctg gttcatgcgt cctatgaccg
    27841 cgtttttcaa acgccggcca tggagaattt gttgctcgcg agttcgccgc agttggattc
    27901 cgtcagcaat tttgttgtga ggctgcccgt acagccagcc aacgcaaatt actatgaaat
    27961 aggattcacc aaatcttttt ccggtaagct gcgtatcgac gccaatattt tccggcgcga
    28021 cttccataat tatcctgacg atgacgtttt actcgaaacc ggcatcagtt ttccaatctc
    28081 tttttcaacg gcaacaattc acggtgaaga aatccaactt tctcttccac actggggccg
    28141 gttttctgga gtgctgagtt actcgaatca aaccggcata ggtcagggac caattacggg
    28201 tggattattt cttggcagcg agacagccgg cattcccgat accagcaagt tcgctgtctc
    28261 tcaggaccag agaaacaccg tgcgtgcacg cgtgcgctac caggcgacgg accgcttgtg
    28321 gttcgctacg tcagcggaat acggcagcgg cttgcctgct gatttgaatg gccccgccga
    28381 tcccagccaa ttcaatttct tgctgcagca atatggccag gccattttga atgaggtgaa
    28441 ctttgatgcc gggcgagtcc gtccaaattt cgcgttggat gccgcagcgg gcgcgacgct
    28501 ctaccataaa gaaggaaaag acgtctcatt cgaaatcgaa ggtcacaacc tgacaaacaa
    28561 attaaatgtc gtgaatttcg ctagtctgtt ttccggcaca gcagtagcgc cgccagccag
    28621 tgtaagcgcc cgcctgaaat tcggattttg attaaagtag cataggcttc aacccgtgag
    28681 agttgggcct tgcaacgacg aaaacccaca ggctgaagag tctgtgtcag aactgcagaa
    28741 tctttgtggg tcgtggcttt agccacgaca tgagtcctgc aaaatgagtg cggctttagc
    28801 cgctgaagct tcaattttgc agttctcaca cagactctga agcctgtgcc accaaaaccg
    28861 gaaccaagtg cgaaacaaca aatttgacag gaccgtgacc gcgacttacg attcttaagt
    28921 taaatgtttc ccttcaagat cactcaaatg aatcgtccta acacaaggtt gtcagttcgc
    28981 cgcacgcgct ggcgcacata ttgcctgggc gcgattttta cacttctcgc cgctcgcgct
    29041 gccgccgata cgaataacgc cgatcccaaa cgctacctcg atgacgtgaa agcgctctcc
    29101 tcccccgcaa tggaaggccg tggggcgggg accaagggta tcgacctggc cgcgaacatg
    29161 atcgagcaac gctatcggtc actgggactg caaccggcgg gaagcaaagt atttcttcag
    29221 ccattcacgg tgattaccgg cgcaaaattg aaggaaggca atcgcattga aattgaagat
    29281 aggaaatcga agcggggatt gaaactgaat caggattttg ttcctttcag tttttcagct
    29341 tccggtgaac tttctggccc cattgttttt gcaggctacg gcgttacggc tccagaattc
    29401 ggttatgacg attacgcgca tctcgacgtc aaagataaaa ttgtcgtcat tctgcgttac
    29461 gaaccatcag gattcgccgc gaaaagcggc aacgccggcc tgactcaaca ctctcagctc
    29521 attactaaag caatcaacgc ccgaaatcat ggcgcgaaag ccgtaatact ggtcaatggg
    29581 aaagtgagag atggggaaga ggatcaactc acaaaattcg gcagcgtgaa cgggcccgaa
    29641 aatgtcggga caatgatcgt gcaggtaaag aacgatgctg caaacgcatg gttcatcgat
    29701 gccggcaagt cgctcgtgga cattcaatct cagatcgatt cgtcttcaac tccgtcgtct
    29761 ttcgcattcc ccgataaact tcagatttct ctcggcgtaa atatcgagaa gacgcgcgcc
    29821 acagtaaaca acgtgctcgc ctatctacca ggcaaaaccg gtgagtacgt tatcatcggc
    29881 gcgcattacg atcatcttgg ttacggaaac attgactcgc tcgcgccatc gcaaattggg
    29941 aaaattcatc ccggcgcgga tgacaacgcc tccggcaccg ccggcgttct cgaacttgcg
    30001 cgcctgctcg cgccccgaaa aggccagctg cagcgcggcg ttctctttgc ggattttgcg
    30061 ggagaagaac tcggtctgct cggttccgcg gaatgggtga aagaaccgac gttgccgctc
    30121 gataaagccg tcgccatgat caacatggac atgattggcc gcatcaagga tgaaaaagta
    30181 tttattggcg ggacgggaac cggagcagaa tttaaagatt tgctcaatga agaaaagagc
    30241 aagtctccat tccactttga atattccgcc agcggctatg cggcgagtga ccacacctct
    30301 tttgtcggca aacaaattcc tgtgcttttc tttttctcgg gcttgcattc ggattatcac
    30361 aaaccatccg acacatggga caagatcaac gcacctgatg cggcaaagct gttggatttc
    30421 atcgaggacg tggtgttgaa agttgactct gaaaaagagc gcgtcgcctt cgttacggtg
    30481 aaggaagatc agccggaagg acatgccgcg agcggtggcg gtggttacgg accctatttc
    30541 ggttccatcc cggatttcgg ccaggaagaa aacggagtaa agttttccga tgtccgccca
    30601 ggatcacccg ctgcaaaggc aggctttaaa tcaggcgata tcctgatcca gtttggcgat
    30661 aaaccaatcc acaatcttta cgatttcacc gatgcgctgc gacgcagcaa ggtgggcgat
    30721 gaagtggaag taaaagtatc gcgcgatgga aagcccgtca gcgcaaccgt aaagttggag
    30781 cagcgaaaat aattctgtag cttacctctc ttgtcctgag tgaaaacgaa ggatcagagg
    30841 tgagggttta aagcaaagtc cgtcaagcag tcaaaatcgg acggtgggtc cgggcaacgg
    30901 tcgcaaaatt ccccgcgtct gaagacgcgg gctacaaaaa tcgggcaaac agatcgaaga
    30961 cgaagaaaaa caaccatgaa gaaatcgcaa ttcgtccgcc ctgcactctt cctgctgtta
    31021 tttacagcag ccgcatcttt agcgacgtac tcgcaagcgc ctgtacaagg aaaaagcagc
    31081 ttagactcgc tcacggtacc cggagaagag cacctcggca atgtccgcca gctaacgttc
    31141 ggcggcacaa atgccgaagc ctatttttcc gccgacgata aatcgctgat tttcatgcac
    31201 tccggcatag gcgtaccatg cgatcaaatg tatacgatgc aaatcgatat gccgggcgga
    31261 aagccctccg tgccgaaact cgtgagcaca ggcaaaggac gcacaacgtg cggctacttt
    31321 ttcccgaaag gcgaccgcat tcttttttct tcgacgcatg agaccagcgc cgaatgcccg
    31381 ccgcgccctg attactcaca cggctatgtc tggccaattt atagttcgta ccggatttac
    31441 accgcgaagc ctgatggcag cgatctgcgt cttctaaact atgcacccgg aagctacaac
    31501 gcggaatcga cgatcagccg tgacggcaaa aaaattgttt tcacatccac acgcaacggc
    31561 gatctcgata tttacacgat gaatgcggat ggcagcgacg tccgccaact cacgcatgaa
    31621 ttgggctatg acggcggcgc atttttttcg gacgacggca aaaaaattgt ctatcgctcc
    31681 gagcagccaa aaaccccaga gcaaattgcg gactataacg agttgctttc tcgcggcttg
    31741 atccgtcccg gcaacctcga aatctgggtg atggacgccg acggcaacaa caaacaccag
    31801 atcacgcaca atggcgcggc aaactttgct ccctattttt ttcctggcgg caaacgcatc
    31861 attttcgcct cgaacatggc caaccagaaa gaccctagcg gctttgacct ctacgctatc
    31921 aacgtggacg gcacaggtct ggagcgaatt acttttcacc cggacttcga tgcgtttccc
    31981 atgttttctt ccgacggcaa acgcctcgtg tgggcctcga atcgcaacgg caaagcccca
    32041 cacgaaacga atattttcat cgccgactgg gtcgaataga ttcgtttttt cgccgcatcg
    32101 ctcggcagcc gctcttctat tttgtaaatc ttcgcgctga cgaatttcag cccgaagaat
    32161 ttttccgcga ttttcccatc ataacttcca tccaaaaccc atagtgagca atcgctcgcc
    32221 aaaatcattt cgagatgaca agccagtaca cgcaaaataa aagcggcgtt gcgaacaatc
    32281 cggaacgccg cgcggtagac agactgggac aacgacgtct ggctcagaaa gtgaacttgg
    32341 cgttgatctg caactggcgt gccccgtaaa gcgcgccaga cgcaatattc gttgatttgc
    32401 tcgcgagtga tataattcca tgcttctctc acattaggtc acgtaagttg cacaatgaaa
    32461 aggagcccgg catgtatcga agcgacgtga ttggcagcct gttgcgaccc acgtatttga
    32521 aaaaagcgcg cgaagaattt gcggcaggtc atctctctga cgcggaattc aaacgcattg
    32581 aagatcgcgc tgtcgatgaa tccgtggcac tgcagcttcg cgcgggcctg gacgtgctga
    32641 ccgatggaga aatgcggcga tacgcttttt acgggcacat gattgacgcc atcgaaggct
    32701 ttgacaagta cggcggctgg tccatcccgt ttcgtaatga aaagggagag gagacaaatc
    32761 atccaaggcc ggtggtggtt tccaaactac gccgcaagcg tcacatgtgc gcggaagaat
    32821 tcacctatct gcgcgcgcgt acggatcata cggcgaaaac gactctttta agtgctcagc
    32881 aggctgccgc ttattatgac gcgaaaaaat ccagcggtgt ctatcccaca gtcgattcgt
    32941 ttcttgccga tctcgttgac ctgttgcgcg aagaagtagc ggaactcgtg cggttagggt
    33001 gcacctacat tcaagttgat tcgccgcagt atgccgcgct gatcgattct tcgattcgtg
    33061 aaggttaccg tcagcgagga aatgatccag accggctgct cgatctctcc atcgagatgg
    33121 acaatgccgt gatcgccggc cacccccatg tgacttttgg cctgcatctg tgccgcggaa
    33181 ataatcaaag caaattttat gcttcgggcg attacgcgcc catcgccaaa gttttccgcg
    33241 aaaccaaatt ccaaagattc ctgctcgaat tcgatgatga gcgttccggt ggtttcgagc
    33301 cgctttcaca cgttccttcg gatcgcacgg tcgtgttggg actcatcagc accaagaaag
    33361 ctccgcttga agcacaggac gaaatcaaaa gccgcattca tgcagccgaa aaatacgttc
    33421 cctttgaccg gctcgcgctg agtccgcagt gcggctttgc ttcgacgatg gaggggaatt
    33481 tgctctccga ggcggatcag gaggccaaac tgcgccttgt ggtcgaaacg gcccgagaag
    33541 tttggggcga ggagaagtct tccctatcat cgaaggtgaa agggaagtct gtctaagttg
    33601 aatcttcctc cgggtccaaa gggttttcct gtgctgggta gcgtgttcga gccgaagggc
    33661 gatacgatca cgtacctgac aaaatgcacg cgcgaatatg gcgatattgt tttttttcgt
    33721 tttctgggcg tgcctgcctg ttatctgagc aggcctgaat acatcgagtc tgtcctcgtt
    33781 acgcaaaaca ataattttgt aaaatcgaaa gattaccgcg ccatgcgccg cgtacttggc
    33841 aatggcttgc tgctgagcga gggcgatttc tggcgacggc agcgcaagct catccagcct
    33901 gcgtttcacc aggaaaaaat ctcggcctac gcgcaaatta tggtggatta cacccagcgc
    33961 atgctggaaa cctggaacga tggccaagcg ctggacatgc acgaagccat gatgcggctc
    34021 acgctgggaa ttgtggctaa gaccttgttc gatgcggacg tttcgcacga agcggaagat
    34081 gtggatgcgg cgctcaccgt actgatggga aaatttctgc gacaggcggg aatggcgttg
    34141 ctccttccaa cttgggtacc tcttccgacc tcgcagcttt tgaagcgcgc ggtcggccgc
    34201 ctagataaag tcatctacgg gatcatcgag cagcgccgcg ccagcggaca aatgtccgga
    34261 gatttgctga gcgcattttt acagacgcgt gatgatgaag gcgtcggcat gaccgatcgc
    34321 cagctgcacg acgaaatcat gacgcttttt ctggcaggcc acgaaaccac cgctaacgtg
    34381 ctttcctgga cgtggttgct cctgggccaa aatcctgaag tggaacaaaa gctcatcgag
    34441 gaactcaacc gagtgttgaa tggaaaaatc cccacgcccg cggatttgcc gcgactcgtc
    34501 tacacagaca tggtgctgcg cgaatcgatg cgattgtacc cgccggtgtg ggtcatcggg
    34561 cggcgcgcac tttctacttt tcggttgggt gattatgaaa ttccggcgaa taccaatatg
    34621 ctaatctcgc aatatttaat gcaccgcgat gcgctatatt ttcctgagcc ggaacgcttc
    34681 gacccgagtc gatggagtcc gaccgatccg cgcgctgcgt ccttgccgcg attcgcctat
    34741 tttccctttg gtggaggccc aagagtgtgc atcggcgcag gtttcggcat gatggaagcc
    34801 gtgctgctcc tcgccacgat cgcgcagaaa tttcaaatgg aagtcgcgcc cggcgaaaaa
    34861 gttagtatgc agccgtcagt cactctccgc cccagaaatg gaattccaat gaagctgaag
    34921 cggagatagg ttttccgtgg cataggcttc agcctgtgga ttttagaact ttcaagacct
    34981 aacccccaca ggctaaagcc tatgccactt aaacccctat cgttcgtcct ggtacgaggt
    35041 aattcaccac ataatcccgc accgcatctt caagcgaagt aataggctga tcgtagccag
    35101 tcgccagcaa cttttgtatg tcggcctgcg tgaaatattg atacttgtcg cgaattactt
    35161 ccggcatatc gacgtattcg atgtgtggtt cgcgtccaag cgcggtaaaa acagcgtgag
    35221 ccagatcatt ccacgtgcgc gcctgtcctg atccaatatt aaagattcca ttcgcattcg
    35281 gatttgcggc cagatgtaaa gtcatgtcta cagcatcctt tacataaaga aaatcgcgtc
    35341 gttgctcacc atcacggtac tcaggacgat ggcttttaaa cagccgaatc acgccggtct
    35401 tcagcacatc ggcataactt ttgcagacca gactgcgcat gtcacctttg tggttttcat
    35461 tcggcccaaa cacattgaaa tatttgagcc ctacaatgcg atcgagccag ccttcctgta
    35521 aagcatgcaa gtccatccac tgtttggatt gcccgtaaag attgagcggt ttcaattgct
    35581 gcatttgaat cacatcggta tcgtccatgc ccgcgctgcc atctccgtaa gtagcagcgg
    35641 aagaagcgta aacaaaacgc acacccgcgc cgagcgccca ttccgcaagc ctgcgcgaat
    35701 actcaaaatt attttcgcgc aggtatgttt cgttcgtttc cgtcgtggaa gagcacgcgc
    35761 ccaaatgaaa tatgcagtcc agatgcccca acgacccgga agcaagtcgc gcatgtaatt
    35821 ctgggactag aataaattct gtatattgta ggcctgccag gttgaatctt ttttcgccat
    35881 ttacatcaaa atcagcaagc atcacttgag tgcatccacg ccgattcagt tcccaaacca
    35941 gcacgctgcc aataaatcca gctgcgcccg tcacaagaac gcgcttgtct ttccaatttt
    36001 cattcacgaa agtcatacag gccaacaagg ttgccacggc aagagaattg aatcaatcgg
    36061 atctgtcctg ttgaagtgat caacaattgc gacgtcttga gctttctgaa tccctgcgct
    36121 agagtaaccg ctcccaggga ggtgattcgc ttgcatggtc acggacaaga acatctgaaa
    36181 gttttattgc gcagcagcgc cattcaaaag cgcgtgcgcg aaatgggcgc tcaaatttcc
    36241 aaggattata aaggtcagcg cgtgcacctg atcggtgtgc tgaagggcgc ggcaattttt
    36301 ctttcggatt tggtgcgtca gataaaggtc gaggtgtcgc tcgattttat gtccgtttcc
    36361 agttacgggc acggcagcca gacttccggc caggtgcgcc taaacaaaga tctggatacc
    36421 agcattgaag gacttaacgt aatcgtagtg gaagatattc tagacacggg cctcactctt
    36481 ggctacctgc ttcgcgtgct acaacagcgc aggccgcatt ccttgaagat tgccgcgctg
    36541 ctggataaac cggagcgccg catcaagaaa gatgtcaagg ccgactacgt cggattccag
    36601 attccaaatg aattcgtcgt gggatatggg ttggactatg cggagcgcta tcgcaatttg
    36661 aaagacgttt gcgttttgtc gcttcctccg tcgtgatgcg atttgtcctg gtccgattgt
    36721 cgttgaaatg tgattcgcgc atcaacgcag ttttgtagcg ccggcgtcac aaaaagtatg
    36781 aaaccactta tcacagccgc cgacctggaa cgcgtcccgg aaaacggtga acttgctgtc
    36841 acggacgata cgatcgtcac tcctctcgcc cgtgatgcgg ccgagcggcg cggcatcacg
    36901 attcgaattg cttcgcagcg tgaacagcaa tcttctgcca atttgtctgc tggcgatatt
    36961 tcgtcagaaa aatctgcgcg tatcgtcgcg attggctccg atcacggcgg tttcgaactg
    37021 aaagaacagt tgaaggggca tctgcgcgat tggggttatc aagcgctgga tttcggcaca
    37081 aacagctcgg aagccgttga ctatcctgat tttgcggaaa ccgttggcaa cgccgttgcg
    37141 aaaggcgaag cgtggcttgg gattgtgctc gatagcgcgg gcatcggcag ttcgattgcg
    37201 gctaacaagg tgccaggagt gcgcgcagcg ctatgctatg atcgcgcgac ggcgcgcaac
    37261 agccgcgagc acaatgatgc caatgtgctg accctggggg cgaagttgat cgctccggaa
    37321 gccgcacgcg aaattgtcgc gctgtggcta tcgacgccgt ttgccggagg gcggcaccag
    37381 cgccgcgtgg acaaaattcg agccatcgaa gagcgcgcag caaagcggca atcggaatag
    37441 caatgcagcc gccgccgggc cggtgcattt tgtagcacgc ctattcagag gcggggtttt
    37501 ctctaacgga tgcggccgac ataaatttca caaagctttt tcaaccgcca cataagggca
    37561 aatcgagtgg accagagcga atttgaacag atcgtacgaa tcgtcaccga agaggtagtg
    37621 cgttacctcg gcgcgtccgg taatgcgaat gtccagcgcg ccgcgaacag cgacagcgaa
    37681 atttgccccg actgcgacct gaactgcgca gagaaatgtg ccaggaaaac ccgcgcagtg
    37741 gtcgaggccg gggccgatcg catctcctgc ggcccatccg tcgtcgaact ggaatccgga
    37801 tttgcgggcc tgatcgatca caccctcttg aagcccgatg cgtcgcggga tgatattcgc
    37861 aaactttgcc aggaagcagt gcgttttgga tttgcttccg tctgcattaa tccttggaac
    37921 gtgcctctgg ccgcggaact ggtgcgcggt acgaaggtaa aagtctgcac cgtgattgga
    37981 ttccccctgg gcgcgacgct ttcccaggta aaaattcatg aagctgagga agccatcaaa
    38041 gttggcgcgc aggaagtcga catggtgatc aatattggcg cgttgaaatc cggacaggat
    38101 gatgtggtgg aatccgatat tcgtggagtg gtcgaagcgg cgcatcgcgg cggcgcaatc
    38161 tgcaaagtaa ttttcgaaac gtctttgctc acggttgagg aaaaagtacg cgcggcgctg
    38221 gcaagtaaaa gagcgggagc ggacttcgtt aaaacttcga cgggcttcag cacaggtgga
    38281 gcgacggctg aagatgtggc gatcatgtgc gccattgtgg ggtcagagat cggcgtaaaa
    38341 gcttccggcg gcgtgcggac gtttgacgat ttgaagaaaa tggtgtgtgc cggcgcaacg
    38401 cgaatcggcg ccagcgcgag tgtaaagatc atggaacaag cggcgggacc ggcgtcatca
    38461 cctgccttaa cttctggagt tcctgcctcc agttcgtatt aattcttacc tgatattgtt
    38521 tccgtcatgc gattcagaaa gcttattgat cgcgccgaga agatgcgaat cctcttctgc
    38581 gctcggcatc taaatccccg agtctgaagt ctcggacaac aacaactctg cgatcgaccg
    38641 atgtttttgt tgcggtcccg ctggtcttgt aacccgattc ttcagagacg ggaattttcg
    38701 taaactattg caataaaatc tgttggagcc tttgcgcatc ctgaaaaaac cacaaagctc
    38761 tgcacagagt tcacctgttt tttacgaggg cgcaatcttc acctgctata ctatgtgact
    38821 cttggggagt tgcgttcgtc cgttccgggc gcagcgcgca attgtgaatt atttaattat
    38881 ggatcccgta caaactccaa cgccgctgaa tgttccggcg gaagttctcg caacgctggt
    38941 cgaaatcggc gaggaaatca attcctcgct gaacctcgac gaagtgttga aaaaaaccgc
    39001 cgcgctcgta aagcgcctga tcgatttcga aattttcagc gtgttgctcg tcgatgaatc
    39061 gatgcatagg ctgtatcacc gattcacgat tggctacggc gaagaggccg gcaaagaatg
    39121 ggtgatcccc atcgggcagg gaattaccgg aacggctgct gcgaccggtc gcgctgtgcg
    39181 cgtgcccgat gtgcgcgaag atccccggta catcaacatt atcgattcgg tgcgatctga
    39241 aatggtgatc cccttgatgg tgaaggggca ggcgattggt gtgctggaca ttcaaagcac
    39301 acaggtcgac tatttcacgc gtgatcagca aagcgttctt acgctcatgg ccagccgttt
    39361 ggcgacggcc atcgaaaatg cccgcctatt tgagcgtatt cgcagccagg cggacacact
    39421 gctggtgttg aacgaagtcg gccgcgaagc gaattcgatt ctggcagtcg aagaattact
    39481 gcggcgcgcc gcggaactcg taaaacgtgt aatcgactat caaattttgg caattttatt
    39541 gtatgacgat gcggcaaata tctttcgtca tcgtctggac atgaagtatg gccagagcgc
    39601 tcaaggaaag ctgcggatta gtccgaccga gggaattgtt gggacggcca tctctacgca
    39661 gatgcctgtc cgtattcccg acgttactct cgattcgcgc tacatcatgg tgaatccgga
    39721 gacgcgctcc gaaatggcga ttccgatgat tcacaagggc cgcgtcgttg gcgttctgga
    39781 tctcgaaagt ccgcaaataa attatttcac ggacgatcat gaacagacgt tgacgattct
    39841 tgccgcgcac ttggctgtat cgttggaaaa tgcccgcttg tacgaacaac ttgccaagga
    39901 cgaagcgcgc atggagcgtg agttgacagc cgctcagcgc ttgcagaccg ctttgttgcg
    39961 tccggttcct accacggatt acgggctcga tatcactgct cgaattcatt ccgcccgcga
    40021 agtgtgcggc gatttatatg attttctttc ttacggtacg caaaatttgg gtatcgcctt
    40081 aggcgacgtt agtggcaagg gaactgccgc ggccctctac ggcgctgtgg ctacgggaat
    40141 tttgcgcagc ctctcacccc aaaaactgca gcccgcggaa cttttgcgcc tgctcaatca
    40201 actggtgtgc gatcgccgcg tggaagggcg ctacatgaca atttgcttcg ccacatggca
    40261 aaaggggcgg cgcaaacttc gtgtggcgaa cgcagggcag acgcagccgc tgctgtggaa
    40321 agatgggcac gtcgagcaaa ttaaactttc tggatttccc atcggaatct ttgacgatgc
    40381 gacgtacgaa gaatggagtg tgtgcctgag ctctggtgac attctcttat tcttttcaga
    40441 tggcttgacc gaagcagcca accgcgaagg aaaatttttc ggcacgaacc gcattaagga
    40501 tttattggcc gcaaactcgc atatgaattc aagcgacttg gccgatcgct tgctgcagga
    40561 agtcgaggag tttacacagg ggggagccat cacggacgat cgcacgctgg tggtagtgaa
    40621 agtaaagtga gcgcatccgc atcgtattcg agcgcttctc gcgccgcgca tgccgctatt
    40681 gtcgatcctg taacttacac gcccggtttt tcctatcgag gctcgcagct ctattgcgag
    40741 ggcgtatcag tcgcagttgt cgccaaaaaa ttcggcacgc ctgcatatct ctacagccgc
    40801 gcgagcatcg agcaagcgta ccgtcgcatg gattccgcat tcggcgcgat tccgcacacg
    40861 atttgctacg cggcaaaagc aaactcaact ctgagtattc tgcgcgtgtt tgccgcgatg
    40921 ggcagcgcct tcgatatagt ttccggcggc gaattatacc gcttgcgacg catcggcgtt
    40981 tcgggaaatc gcattgtatt ttccggcgtg ggaaaatccc gcgaagaaat ccgggaagcg
    41041 cttcgtgcgc gaattcttct ttttaatatc gagtcgtccg cggagctgga tttgttggcg
    41101 tcagaggcgt ctcggcttcg tgtgcgcgcc gctgcttcca tccgcgtcaa tcccgacgtc
    41161 aaagctggag gccatcgtca catcgcaacc ggacaccata gccataaatt tggaattgac
    41221 tggggaaccg cccgcgatct ttatctgcaa caccgcggtt ctcgctggat tgactggcag
    41281 ggaatcagtg cgcatatcgg ctcgcaaatt ctttccgtaa atcctcatcg ccgcgcactg
    41341 aaacgcctcg caggatttgt gagtgacctt gcaactgcag ggataccgct gaagtacgtc
    41401 gattgcggcg gcggattggg tattcgctac acttcggagc agcctccaga aacagatgtg
    41461 tgggccgcgg aactggtgcg cgttattaaa ccgctgggtt gccatttatt gattgagccc
    41521 ggacgatcgc tgattggccc agccggcgtt ttggtcatgc aggttttgta taacaaagaa
    41581 actcgcggga agaatttcgt aattgtcgac ggtgccatga atgattttat gcgccctgcc
    41641 ctgtacgacg cgacgcatcc catcacgcgc gctcgacgca gttccggatc gcgcgatcgt
    41701 gcggatattg tcggtccggt gtgtgaatcg ggcgattgct tcgttcagga ttggccgctg
    41761 ggaaaagtaa agccgaaaga cgtgctcatc ttgtgggggg ccggagccta cggaactgtg
    41821 caagcttcta actacaattc acgcccgcgt cccgcggaaa ttttagtgtc aggaaacgga
    41881 atgaaattga ttcgtcgccg cgaggctcgc ggcgacctgg ttcgcggaga ataaatcgat
    41941 tgccggccgt ttcgctagcc ttttttatga tgccaacagg gccaagccct gatatctgtg
    42001 gatcgaagct ttagcccgac atcaaaatgc gcagaaaaag agtgcttgag ccccctgacc
    42061 ggcgaccggg cttgaagtcc aggtcattta cgagacgatc tctaattatt ttaaaatcag
    42121 atcggaagag gcacgccgat ggaatccgcc tcccaggcgt tcaccagtgc agtgaggaac
    42181 gtgtctcttt gttccttcgt gcatggaaga ttggaaatat ccgtattcag ccaaaacagg
    42241 caattcatga aaattgcttt gcgatgtacg gaaacctgcg aactgaaatc ttcctggccg
    42301 gccatttttg ataaagccga aaattgttcg atggcattga aatctttaaa accgttctct
    42361 tcttcctggt agccgacaaa ttccgatcca aagaatcctt cttccacgga aatctggcca
    42421 ttgatcgctt tgttaaatac gctgagctct ttcgccggga tccaatattc gcgatgcgct
    42481 gccgatccgg ctgtgtgcaa ttcaaacttg gaaagataag tggaagacac ttcaaacttt
    42541 gtgacgaagc cggagaatcc ggattttgca tccggtgtat tccagtcccg cgcgatttgc
    42601 cgggcgtatt ccaaattcac gacgggatag aaaatcgcct gattttccaa acgttgcggg
    42661 aattctttca ttcctgaatc ccaaagtagc tcgagttcgc gaaggccgat tggccggaaa
    42721 agatttgtag gcataatgtc agcgatggtt gccgcagttc ggcgtgc
//
LOCUS       DASZMB010000074         3197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2551440, whole genome shotgun sequence.
ACCESSION   DASZMB010000074 DASZMB010000000
VERSION     DASZMB010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2551440"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VG272_03560"
     CDS             complement(<1..767)
                     /locus_tag="VG272_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HWF12787.1"
                     /translation="MTHKITLLPGDGIGPEVANAATQIVEAAGVTVEWEVFAAPLKDG
                     ARVGEFPREQAVESVRRNHVALKGPMATAVAQGPPSINVALRKELDLYANLRPVRNVA
                     GVSSRWSGVDIIIVRENTEDLYAGLEHTVVPGVVESLKIITEYASTRIAKFAFEYARK
                     NGRKKIHAIHKANIMKLSDGLFLSSIRTVAEKYPEIEYRELIVDNACMQLVMNPGQFD
                     MLLLPNLYGDVVSDLGAGLVGGLGVVPSGNIGDVAAL"
     gene            1104..1427
                     /gene="erpA"
                     /locus_tag="VG272_03565"
     CDS             1104..1427
                     /gene="erpA"
                     /locus_tag="VG272_03565"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HWF12788.1"
                     /translation="MIQVTPVATTKVKEIMGQQNPSPTALRVAVVGGGCSGFSYHMAF
                     ENAENPTDNVYEFDGLKVLVDQMSEMYLEGVSIDYIETLEGAGFKFNNPNVKSTCGCG
                     SSFSV"
     gene            1719..>3197
                     /locus_tag="VG272_03570"
     CDS             1719..>3197
                     /locus_tag="VG272_03570"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HWF12789.1"
                     /translation="MKIALAQLNPTVGDFAGNSAKILHFARRAQEKGVDLAVFSELSL
                     CGYLPMDLIERPHFIDRNERELACLAKQLPLPCIVGYAAHATGATGKSAANAAALLAN
                     GKVEFVQHKMLLPTYDVFDESRYFQPAATQSVFPFGDQKLGITICEDIWNDKTFWAKP
                     LYERDPVSELAAKHASLIINISGSPYTIGKRALRSGMLRSLAKLHGMPVVYVNQVGGN
                     DSLVFDGGSVVVLPDGRISAQAKSFEEDLVIFDTAALSGEIHSQPTDELEHVLQALIC
                     GTRDYVRKSGFRAIVVGLSGGIDSSVVAAIAVAALGRKNVQGVSMPGPYSSEGSREDA
                     RQLATNLGIDFLTIPITDVFRAYRSTLAGAFEGRPEDVTEENLQARIRGNFLMALSNK
                     FGSMVISTGNKSEMAVGYCTLYGDMAGGLAMLSDVPKTMVYALVEYINRERELIPRAS
                     VEKSPSAELRPNQTDQDSLPPYEILDQILKAYIEELKCPEEIA"
BASE COUNT          751 a          876 c          814 g          756 t
ORIGIN      
        1 aacaacgccg cgacatctcc aatatttccg ctgggaacta cgcccagccc gcccacgaga
       61 cccgctccca aatcactgac cacgtctccg taaagattcg gcagcaagag catgtcgaat
      121 tgcccaggat tcatcaccag ctgcatgcac gcgttgtcca cgatcaattc acgatattca
      181 atttccggat atttttccgc gaccgtgcga atcgagctca gaaacaggcc gtcggagagt
      241 ttcatgatgt ttgctttatg gatcgcatga attttcttgc gtccattttt ccgcgcgtat
      301 tcaaatgcaa attttgcgat gcgcgtggat gcgtactccg taataatctt caaactctcc
      361 acgacgcccg gcaccaccgt gtgctccagc cccgcataca gatcttccgt attctcgcgc
      421 acgatgataa tgtccacgcc cgaccaacgc gaagaaacgc cagcaacatt tcgcacaggc
      481 cgcaaattcg cgtacaggtc gagttccttg cgcagcgcga cgttgatgct cggcggtccc
      541 tgcgccactg cggtggccat cgggcccttt aacgcgacgt ggttgcggcg cacggactcc
      601 acagcctgct ctcgcggaaa ttctcccacg cgggcgccat ctttcaacgg tgcggcgaag
      661 acctcccatt ccacggtaac gcccgcggcc tctacaatct gcgtggccgc atttgcgact
      721 tcaggtccga tcccgtcacc gggaagaaga gtaatcttat gtgtcataga cccaccaatc
      781 ttacgggatg gcgatgcgtt tgtcgaaaat ctcgagcaaa agttgtggca caattgccaa
      841 attgcagctg ggttgccgac gtactgcata actggcaacc gtattgggca cacgttggag
      901 tgatcctagg tccgccgcta ttacatctcc attgatcggc aattaaggca cattcatctg
      961 aaactcccgt gcaataagcg catcaaatct tttatattag cctcggtgcg cggcgtttcg
     1021 cgcaggtttg cgtttctgca agttgtgtta taatgtagca gttcaaaata tagccgaatc
     1081 cggcagggag tgaaggtact gaaatgattc aagttactcc agtggcgaca acgaaggtaa
     1141 aagagattat gggccagcag aatccgtcgc ccaccgcact tcgcgtcgct gtcgtgggcg
     1201 gaggctgctc gggctttagc tatcacatgg cttttgagaa cgccgaaaac ccgaccgaca
     1261 acgtttacga attcgacgga ctgaaggttc ttgtcgacca gatgagcgaa atgtatctgg
     1321 aaggtgtctc catcgattac atcgagacgc tcgaaggcgc cggcttcaaa tttaacaacc
     1381 cgaatgtgaa gagcacctgt ggctgtggca gttcgttcag cgtgtaagaa cgccgccaag
     1441 tttcccatcg ccgcgcattt cgaagggcct ttgcgagttc tcgcaagggc ccattttttt
     1501 gagtgtaatc gaatcttaaa aaagcaaaaa ccgagtggcg agatttcaac tgaaatctca
     1561 aatttgaaat ttgagattta tatttcgtgc cgcaatctgt ttagcctaag tgttccgaag
     1621 ggcggcgtcc ggacatctca gaccgcgcgg aatcatgatc gatctcgtgt tgccctcatt
     1681 gcaatgcgcc acggcgcccc gtatcctata gcgagccaat gaaaatagcc ctcgcccaat
     1741 taaatcccac tgtcggcgac ttcgcaggga attccgccaa aattttgcat tttgcgcgtc
     1801 gcgcccagga aaaaggcgtc gatctcgccg tgttttctga acttagtttg tgcggctatc
     1861 tgccgatgga cctcattgag cggccgcatt tcatcgatcg aaacgaaagg gaattagcct
     1921 gcctcgcgaa acagttgccg cttccttgca tcgtcggcta cgcggcccat gccaccggcg
     1981 ctaccggaaa atccgcggcc aatgcggctg ccctcctggc taacggaaaa gtcgaatttg
     2041 tgcagcacaa aatgcttttg ccgacctacg atgtcttcga cgaatccaga tattttcagc
     2101 ccgccgcaac ccaaagtgtt tttccattcg gcgatcaaaa gcttggcatc accatctgcg
     2161 aagatatttg gaatgacaag acattctggg ccaagcctct ctacgagcgg gacccagtct
     2221 ccgaactcgc cgcgaagcat gccagcctta ttatcaacat ctcaggttcc ccgtatacga
     2281 ttggcaagcg cgccctgcga agcggcatgt tgcgctctct agctaagttg cacggcatgc
     2341 ccgtggtgta tgtgaaccaa gttggcggaa atgacagtct ggtatttgat ggtggcagcg
     2401 tggtggtgct gcctgacggc cgcatttccg ctcaggcgaa atccttcgag gaagatttag
     2461 ttatttttga cactgccgcc ttgtccggag aaattcacag ccagccaacg gatgaattgg
     2521 agcatgtcct gcaagcactc atctgcggaa cccgcgacta cgttcgcaaa tcaggtttcc
     2581 gcgccatcgt tgtaggtttg agtggaggga tcgattcgtc cgttgttgct gcgatcgcgg
     2641 tggccgctct cggccgcaaa aacgtgcagg gcgtgtcgat gccgggcccg tactcctctg
     2701 aaggaagccg tgaagacgct cgccaattgg cgacgaatct tggaattgat tttctaacga
     2761 ttcccattac cgatgttttt cgagcataca gatcaaccct ggcgggcgca ttcgagggtc
     2821 gtccagaaga cgtcaccgaa gaaaatctcc aggcccgtat tcgcggaaat tttctaatgg
     2881 ccttgtccaa caagttcggg tcgatggtga tcagcacggg aaataagtct gaaatggcag
     2941 taggctattg cacgctatat ggagatatgg ctgggggact ggccatgctt tccgacgtcc
     3001 caaaaacaat ggtgtatgca ttggtcgagt atataaatcg cgaaagggaa ttaatccctc
     3061 gcgcttcggt tgagaagtcg ccctccgcgg agcttcgccc taatcaaacg gatcaggatt
     3121 ccctgcctcc atacgaaatt ctggaccaga ttcttaaagc gtacatcgaa gaattaaagt
     3181 gccccgaaga aattgct
//
LOCUS       DASZMB010000075        16698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3096395, whole genome shotgun sequence.
ACCESSION   DASZMB010000075 DASZMB010000000
VERSION     DASZMB010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 16698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16698
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3096395"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VG272_03575"
     CDS             <1..545
                     /locus_tag="VG272_03575"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWF12790.1"
                     /translation="GATLMALGIMISTFGCANGLILAGARVYYAMSRDGLFFHGIGKL
                     NAQHVPAMGLVLQGIWATILVLLRTRLVNATTGVVSFGNLYGDLLDYVVFAVLIFYVL
                     TIGGLFVLRRKRPDAERPYRTWGYPIVPILYIVTASIIMLILILYQTQDAWPGLVIVM
                     LGVPVYWAWTRRSGTENSRL"
     gene            685..2208
                     /locus_tag="VG272_03580"
     CDS             685..2208
                     /locus_tag="VG272_03580"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWF12791.1"
                     /translation="MANQLFQVKTHEALVEELQGENRLHRVLGPVQLSSLGVGAIIGT
                     GIFVLTGVAAHDLTGPALMLSFVVSGLTCFFAALCYAEFASMVPVAGSAYTYAYATLG
                     ELFAWIMGWDLILEYAVASATVAHGWSHYFQDFLRFFHVQLPHEFTNAPFDYSLTSGR
                     FALTGTWFDLPAIVIAMLVTIVLVKGIRESASVNTVIVIVKLAIVFFVIGAGAFYVHP
                     ANWHPFAPFGLSGLSFFGHTIVGMRGPGGQPMGMLAGAAIVFFAYIGFDSISTHAEEA
                     RNPSRDVPIGIMTSLVLCTFLYIAVAAVLTGMIPYSKINIDAPVSDAFRQVGLPWAQL
                     LVSVGAVAGITSVLLVMMLSQPRIFLAMARDGLLPTGFFGAVHERFRTPWKSTILTGL
                     FVATMGAFLPLRILAELVNIGTLLAFVIVCAAVLIMRRTHPEVRRPFRVPFGTLFPIL
                     GILSCLMLMFSLPVANWIRLFVWLAIGLVIYFSYGIRHSHITLQNREANAKLAAERK"
     gene            2339..4387
                     /locus_tag="VG272_03585"
     CDS             2339..4387
                     /locus_tag="VG272_03585"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWF12792.1"
                     /translation="MASSFTTRSRAFWAGLTLVDWVALAIIIIYGVLLGLREYYSHTP
                     QSGFLFFLAICAAIYWLMRGWIWARDNLLWSLRNRLVAAYIFIAVVPVLLLLAMAGLA
                     AYLLYWQLGAYVIHSEMEDHEERIGVVATAMATSYAAEAALGSKAAALALPVAPETYI
                     KNAMTELPGLKVETGVGEELLHANGASGRNRFRGLVFSDGQLALRAVVARPAPTGRIL
                     VSAIVPITPELIETLAPELGPIEFKVLRPDIGERRPQLPIVISTRRYLTEQEIDTTER
                     PIPKSKNPFDKLISGIVGLDVLDLDQKPGHNDPVRVIVYFSTRPSLLNRRLFSPLGDL
                     GSAAATALLVIGAIFLVIEMGSLATGIVMTRTITTAVNSLYTATQHVKEGDLTYRVRI
                     PHRDQLAALGDSFNSMTLSVSSLIDEQGKRQKLENELSIAHEVQQQLFPRSLPKLPGL
                     DIEAICRPARVVSGDYYDFIRISPTRVAIALADISGKGISAALLMANVQAALRSDVLR
                     YRNGQPGIHHEQIDTAEIVSHLNRHLFRNTTDERYATCFFGVYDAETRQLHYTNAGHL
                     PPVYFCGTNVRRLETGGMVVGLFNEVPFQQGAVEIEHGGMLVAYSDGLIEPENVYGEE
                     FGTERLIDLVSRNREKSPHDIAEAMMTAAEEWSGSPEQADDMTVIVMSFSEYSQELRA
                     "
     gene            4384..5979
                     /locus_tag="VG272_03590"
     CDS             4384..5979
                     /locus_tag="VG272_03590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HWF12793.1"
                     /translation="MKVLTAAQMREADRLTTERYGIPGIELMENAGTAIAEFLHEKFA
                     DLRNRKILVLCGKGNNGGDGLVAARHLKNSGCPAVVFLFANPGSVEGDAAANLKRWQQ
                     GLGEMYVVTSEAEWESARPALVEAELIVDALLGTGLRGHVEGLLGKVIDDLNAARAKR
                     RDKMAVVAVDMPSGLASDSQDFGGPVVRADFTVTLTAPKVGQLVLQNSTRCGELVVKD
                     IGTPPELLESDPHLKINWIQAGEFRALPLAREQASNKGTYGHALIVAGSLGKSGAAIL
                     AGRGALRSGAGLVTAATPLDVLPIVAGGMPEMMTAPLVSTDAGTASLRNLDYGKFSEI
                     LRGKSVLAIGPGLSMQHETQQFIRHVVGQTEIPIILDADGLNAFAGMKDTLTERKASA
                     MVLTPHPGEMARLLGIAVKEVQLRRLDVALEAAGRWRAYVILKGFHTILATPSGHAYI
                     NTTGNPGMATGGTGDVLTGILAGLTAQFGVDDWARVLSLGVYLHGLAGDIAASRVGES
                     PLIASDVIDAIPEAYARVLAAAK"
     gene            6132..6605
                     /gene="tsaE"
                     /locus_tag="VG272_03595"
     CDS             6132..6605
                     /gene="tsaE"
                     /locus_tag="VG272_03595"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HWF12794.1"
                     /translation="MSADEIITNSYEETIAKGREIASKLVAPVLVLLKGDLGAGKTTL
                     TKGIISGLGAAREEDVTSPTFNLVHEFRIEHPLAPGQNARACKVYHVDLYRINSFHDL
                     ESLGIEDALSEKAIVIIEWPERFTFRTDWPTIEIHLDHAGGDSRRIAISDQTVKK"
     gene            6752..7708
                     /locus_tag="VG272_03600"
     CDS             6752..7708
                     /locus_tag="VG272_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWF12795.1"
                     /translation="MKIRCVFCVVTIVLGSVVGLGFNSSANSLPQSSFPAPSPIKFDH
                     ALICVSDLAPMQQGLTDVGLKPDYGGHHGHATTQMAQLGFPDGSYIGVEAPVDASVET
                     GTPQSTLMKANAGPCGWSATSTDMKGDLGRISKLGVIVGEPEARSRTRPDGKLAEWRS
                     ASLGPGAPGSNLPFLIEDKGPRSNRAPHASPSTEGTTLSGVGVVVLGVKNLDESIALF
                     RKVYGLPAPKIEEHPEFGAKVAYFAGTPVMLAAAASPDSWVAERIARLDQIPLAFLLH
                     ADDFKAASKHFSLPTGKPWFNLDVSWFDVQKLQGVRLGIVGG"
     gene            7757..8407
                     /locus_tag="VG272_03605"
     CDS             7757..8407
                     /locus_tag="VG272_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWF12796.1"
                     /translation="MTEITRRGFVKGAAVAPLAFSPFSVAQTSSAPHYDVVIVGAGHN
                     SLITASYLAKAGFKCLVLEGRPMIGGGTKTAQLTLSGFEHDVCSSIHARIQANPLMKN
                     NELNLGEYGLEYIFPDPVYHIAFPDGSYITKWRDFDRTCEEIAKYSKKDAETWRRMAN
                     EADEIRPLVNQVEYTPIGFGKSLAERLAEHPKGKIWQRRMAMSKWDVLRQNFEDER"
     gene            8404..9399
                     /locus_tag="VG272_03610"
     CDS             8404..9399
                     /locus_tag="VG272_03610"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWF12797.1"
                     /translation="MMPPFPKDLTGPYSGFSAYPSKREEVPQPKGGSGMIATALSRLL
                     ESHGCEILVNKPVEQLIVENGKCVGVECADGTSYRADKAVLSTVHIKQLVHMAPKEMW
                     PQDFLEGVKTFNTGNASFNSHFATTEPLKFQTKSGTIMPVHISNIQSCKRALRFESEL
                     TAGEFHVEEPVLHIVQTSVADPTRAPAGMHTIRVLCEQAIYDLPNGGPGRWSEIKAEV
                     QLAHLRAVQKIAPNLTDDKILARFSTSPVDIEQMNAAMWHGSCHSGTDGPAQAGAMRP
                     VPGWSQHRMPIPGLYQTGGTTHPGGGVSGGPGRNAAVVMLKDFGTSMEEVLSKKG"
     gene            9778..10104
                     /locus_tag="VG272_03615"
     CDS             9778..10104
                     /locus_tag="VG272_03615"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HWF12798.1"
                     /translation="MSCHSSFGKNRPSIQKLAEYDKSKTPIPWVRVYAVLPGVKWSHK
                     THLDAGMKCEMCHGNVSQMVVMTNVKSVNTMVGCLDCHTLHSAKTTCTTCHPAWAPEM
                     AVDKQK"
     gene            10335..12860
                     /locus_tag="VG272_03620"
     CDS             10335..12860
                     /locus_tag="VG272_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12799.1"
                     /translation="MPERTSMLSPDTPARINLKAYSAELSLRSLAVGVDNIHHDVFLS
                     PLFAETTEAYLLEFIRQTVNLPFLSQKDRSPTERRTSPRREADKKNARSQEVASWKKQ
                     LSDLLHSGLQKAKFEKNIEIDLLLRATLLKYLTQEIGAQFANILLEAKEWIRGRGEHF
                     DRSEQAHVIKASLAELQADRRNLFRKVGQHVYQAILDIEDNSLGRSRKALFGDEASGS
                     YDILNNRLAFVENGKDDVLFLEQYVLLGNYIRDQDRFETFDTVMLDLLRESVLTGDSG
                     NDLNETWQSHQKLVDEAVAYQAEIARLKDERESLVRKLERSESLMGRVGFGSDPASIR
                     ASMAEIEKRQRFAQRKLEETNPRIEASHNEADYLAKQYQERLGDYLNQPENARSLFDP
                     AATSGANKGRAGLRAQLLEELVVRLEQRDLLVHILASYQLRNICHDYCPPIHLQQLKK
                     ALVSREELKRVEEILKQFPARQYSAQRIEELSKKIRKTPREEVRAVVIRFAEDFMRLR
                     RDTRNYERLSAAMERINLVRNERRRELSEMNHSLYEFLLPEEARPAEDHVVTHTVIKA
                     DVRGSTKITQDLLERGLNPASLFSLNFYEPVKRILDRYGASKVFIEGDALVLAIYETE
                     STRAQQRAVSKACALARQILAVSTAYNDRPDTNDLPRLELGVGIAFQGSAPTYWMDTD
                     SRIMISRALNLSDRLSSCSKAAKRMLAQNPSPFRLFLFQTMMDGVAEDEADEFLVRYN
                     LNGVELNEEGFQKLQEEISLAELEVDCIMPWGKERVTFFRGETPVGEGLEPVLIRRGF
                     IREMLPGGKIGQAGTRPYYEVCVSPKVLELAEFLAETVANRRA"
     gene            12940..13101
                     /locus_tag="VG272_03625"
     CDS             12940..13101
                     /locus_tag="VG272_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12800.1"
                     /translation="MSRSKSKSKPRKFTAAREARRRARELAGPPPAARVIPDKRLKPS
                     KHKKKLVEE"
     gene            complement(13260..14528)
                     /locus_tag="VG272_03630"
     CDS             complement(13260..14528)
                     /locus_tag="VG272_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWF12801.1"
                     /translation="MAKFIRYTKYTGEPADSVDLQELLKRLSDFFLQSGFESQFYGLS
                     EMDPEKSMEALRQAIQRALEQGDLLGDQDMKDLLDGTPEQMDAKMKELIDQLIEKLME
                     EGYVTGQPQITAPPDKTPRGQVGQGRERDRENEPRFEITDKTIDFLGFKTLKDLMGSL
                     GKSSLGRHDTRDLSTGVESGGISRAYEFGDTLNLDISATLFNAVRREGSKVPIELDYP
                     DLMVHQTEYQSSCATVLMLDCSHSMILYGEDRFTPAKKVALALSHLIRTQYPGDSLQL
                     VLFHDSAEEMPLAELARVQVGPYYTNTREGLRLARRILARQKKDMKQIVMITDGKPSA
                     LTLEDGRIYKNAFGLDPLVVTQTLEEVGRCRKSGILINTFMLASDYSLVQFVQKVTEL
                     CRGKAYFTTPYTLGQYLLMDYMQKKTRHIH"
     gene            complement(14631..16112)
                     /locus_tag="VG272_03635"
     CDS             complement(14631..16112)
                     /locus_tag="VG272_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12802.1"
                     /translation="MTTKPGTLGELKAFPSHAPGTARRTVKDELRANILKKLEAGEPL
                     FPGIHGYEETVLPQIANALLARHNFILLGLRGQAKSRILRGLINFLDTEMPYITGCEI
                     HDNPYAPICKACRDLVLEQGDATPISWLGRDARFIEKLATPDVTIADILGDVDPIKAA
                     RSGRALGDELTIHYGLLPRANRGIFAVNELPDLAGKIQVGLFNIMQEGDIQIKGYPVR
                     MPLDVLLVFTANPDDYTARGKIITPLKDRIGSEIRTHYPATVEEGVSITKQEAWVARD
                     GGRSAVDVPNYLREVVEFVAFLAREDKRIDKRSGVSQRLPIAALESVVSSAEQRAVRN
                     QESRSSARVADVYAALPAITGKMELEYEGELRGAENIARELIRNAVGKVFTNRMEGAN
                     LQPIVQWFELGGELKIPENAPAADALAQLKKIQGLMDNVERLGVDGRSGPAGMAAAAE
                     FILEGLWAHKRISRNEERGFHAERPKVPDPHEQQQRPPRRQFN"
     gene            complement(16258..>16698)
                     /locus_tag="VG272_03640"
     CDS             complement(16258..>16698)
                     /locus_tag="VG272_03640"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HWF12803.1"
                     /translation="ALIVKVLKEYEDGRSDILTAGQSAFRLIRMHEEKAYMEADILFL
                     EEDFTGVDSEVSEQLEDLCNQCHGLLYGEDAPHFETEGGISLAYHVATELPLDTGMRQ
                     SLLEIRSESERQARLVAHLQEWYPQLKKREHVRGKAAGNGHSKL"
BASE COUNT         3669 a         4313 c         4828 g         3888 t
ORIGIN      
        1 gcggcgcgac gctgatggcg ttggggatca tgatttccac gttcggatgc gcgaatggac
       61 tgatcctggc cggcgcgcgc gtttactacg ccatgtcacg cgacggatta ttttttcacg
      121 gaattggaaa gctgaacgcg caacatgttc cagccatggg tctggtgctg cagggaatat
      181 gggccacgat tctggtgttg ctcaggacgc gcctcgtgaa cgcgaccacc ggcgtggtga
      241 gctttggaaa tctttacggc gatttgctgg actatgttgt tttcgcggtg ttgatttttt
      301 acgtgctcac aatcggcggc ttattcgttc tgcgccgcaa acgcccggat gccgagcgcc
      361 cttaccgcac ttggggatat cccatcgtgc caattcttta cattgtgacg gcatccatta
      421 tcatgctcat tttgatcctg tatcagacgc aggatgcgtg gccgggcctc gtgattgtga
      481 tgctcggagt gcctgtgtat tgggcatgga cgcgacgttc cggaacagaa aactcccgcc
      541 tctgaccctt cgcattctct tagggcaaga gaggcgggct gctgtagccc gggtcttcag
      601 acccggcgat ttgctgagtg tgaggcgcag acctttccaa tttgagaatt aatgacctga
      661 accatcaacc ggtaaaaggt ggatatggcc aaccaattat ttcaagtcaa gacgcacgag
      721 gcgctggtcg aggaactgca aggcgagaac cgcttgcatc gcgtcctcgg gcctgtgcag
      781 ctatcgagtc tgggcgtcgg agcaattatt ggcaccggaa tttttgtact gacaggcgtt
      841 gccgcgcatg atttaacagg gcccgcgctg atgctctcgt ttgtggtgtc cggcttgacg
      901 tgttttttcg cggcgctgtg ttacgcggaa tttgcgtcga tggtgccggt ggccggctca
      961 gcatacacgt acgcatatgc gacgcttggc gaactcttcg cgtggattat ggggtgggat
     1021 ttaattctgg aatacgcggt ggcgtcagcg accgtggcgc acggatggtc gcattacttt
     1081 caggattttt tgcgcttctt ccacgtccaa ttgccccatg aatttacgaa tgcgccgttt
     1141 gattacagcc tgacgtcggg acgattcgcg ttgacgggaa cgtggttcga tcttcccgct
     1201 atcgtgattg caatgctcgt taccatcgta ctggtgaagg gaatccgcga aagcgccagt
     1261 gtgaatacgg tcatcgtgat cgtgaaactc gccatcgttt tcttcgtgat tggcgccggg
     1321 gcattttatg tgcatccggc gaattggcat cctttcgctc cgtttgggct gtcgggcctc
     1381 agcttcttcg gtcacacgat tgtggggatg agagggcctg gcgggcaacc aatggggatg
     1441 ctggcgggag cggctatcgt attttttgcg tacatcggtt tcgattcgat ctccacgcat
     1501 gccgaggaag ctcgcaatcc ctcacgcgat gttccaatcg gaatcatgac gtcgctggtg
     1561 ttgtgcacat ttttgtacat tgccgtagca gcggtgctga ccggaatgat tccatacagc
     1621 aagatcaata tcgatgctcc ggtctcggat gctttccggc aagtgggtct gccatgggcg
     1681 caacttttag tttcggtagg ggccgtcgcg ggtatcacgt cagtgcttct cgtgatgatg
     1741 ctcagccaac cgagaatttt tctggccatg gcgcgcgatg gtttgctgcc gacgggattt
     1801 tttggcgcgg tgcatgaacg gtttcgcacg ccgtggaaat ccactatcct cacgggattg
     1861 tttgtcgcga ccatgggcgc ttttctcccg ctgcggattc tggccgaact cgtgaacatc
     1921 gggacgttac tggcgtttgt gatcgtttgt gcggcggtgt tgatcatgcg gcgcacacat
     1981 cctgaagtac gccggccatt tcgagtaccc tttggaacct tgtttccgat tcttggaatc
     2041 ctttcctgct taatgctgat gttctccctg ccggttgcaa actggatccg cctgttcgtg
     2101 tggctggcga ttggactagt gatttatttc agttacggca ttcggcacag ccacatcact
     2161 ttgcagaatc gcgaagcgaa tgcaaagctc gcggcggagc gaaaatagcc ggatacggcg
     2221 gcagcgcgga gattgtcgcg ttcagggccg catgatcgaa taatttttca gtaatctcct
     2281 aactcattta ctgcggtagt attcttttgg gtagctgcgg aagcttgcct gtaaaagaat
     2341 ggcctcatcc tttacaaccc ggagccgcgc attctgggcg ggcctgacgc tggtcgattg
     2401 ggtagcgctc gcgatcatca ttatttatgg agtgcttctg ggattgcgcg aatactactc
     2461 acatactcct caatccggct ttttattttt cctggcaatt tgcgctgcaa tttattggct
     2521 gatgcggggg tggatctggg cgcgtgacaa ccttctgtgg agtctgcgca acaggctggt
     2581 tgcggcttac atttttattg cggtcgtgcc cgtactgctt ttgctggcga tggccgggct
     2641 ggcggcgtac ttgctgtact ggcagcttgg cgcgtacgtg atccattcgg agatggagga
     2701 ccacgaagag cgcataggcg tggtggctac cgccatggcg acgtcctatg cagcggaagc
     2761 agcgctgggc agcaaagcgg cagcgctcgc tttgcctgtg gctcctgaaa cttacattaa
     2821 aaacgcaatg accgaattgc ctgggctgaa ggtcgaaacc ggcgtgggag aggaactgct
     2881 ccacgcgaac ggcgcgtcag ggagaaaccg ttttcgcggc ctcgttttca gcgacgggca
     2941 gttggcgttg cgagctgtcg tcgcacgccc agctccgact ggacgcattc ttgtttcggc
     3001 cattgtcccc atcacgccgg aattgattga aacgcttgct ccggaactgg ggccgattga
     3061 attcaaagta ttgcgaccgg atattggtga gcgcagacct caacttccga ttgtgatcag
     3121 cacgagacgg tatttgaccg agcaggaaat cgataccacc gaacgcccca taccgaagtc
     3181 gaaaaaccct ttcgataaat taatttctgg aattgttggt cttgatgttt tggatctgga
     3241 tcagaaacct ggacacaacg atccggtgag agtgatcgta tatttcagca cgcgaccgtc
     3301 gctcctgaac cggcggctgt ttagtcctct gggagatttg ggaagcgcgg ctgccacggc
     3361 gctgctggtt ataggcgcaa tatttttggt aattgaaatg ggatcgcttg ccacgggcat
     3421 tgtcatgacg cggacgatta caaccgcagt gaacagcttg tataccgcca cgcagcatgt
     3481 gaaagagggc gacctaacgt accgcgtgcg tattccgcac cgcgatcagc ttgcggcgct
     3541 gggcgattct ttcaattcca tgaccctgtc cgtttcctcc ttgattgatg agcaaggcaa
     3601 gcggcaaaag ttggagaacg aattatcaat cgcacatgaa gtgcagcagc aactttttcc
     3661 gcgctcactg cccaaactgc ccggcctcga tattgaggcc atctgccgcc ccgcgcgcgt
     3721 ggtgagcggc gattactatg attttattcg catttcaccg actcgcgtgg ctatcgcgct
     3781 cgcggatatc agcggcaagg gaatctcggc ggcgctctta atggccaatg tgcaggcggc
     3841 tttgcgcagc gatgttcttc gctaccgtaa cggccagcct ggcattcatc atgaacagat
     3901 tgacacggcc gaaatcgttt cgcatctgaa ccggcattta ttccgcaaca caaccgacga
     3961 gcggtacgcc acctgttttt ttggagtata tgacgcggag acgcgccagc ttcattacac
     4021 gaatgccggc catcttcctc cagtctattt ctgcgggaca aatgtgcggc gattggaaac
     4081 gggcgggatg gtcgtcggat tgttcaacga ggtgcctttt cagcagggcg ccgtggaaat
     4141 tgaacatggc ggcatgctgg tggcttacag tgacggcttg atcgagccgg aaaatgtgta
     4201 tggcgaggaa ttcggcaccg aacgcctgat tgacctggtc tctcgcaaca gggaaaaatc
     4261 cccgcacgat attgccgagg ccatgatgac cgccgctgag gaatggtccg gctcgccgga
     4321 acaggcggac gatatgaccg tgatcgtcat gagcttttcc gagtactcgc aggagttgcg
     4381 cgcatgaaag tgctgacagc ggcgcaaatg cgcgaggcgg atcgtctgac gacggagcgg
     4441 tacggcattc cgggaattga attgatggag aatgccggaa ccgcgattgc ggaatttttg
     4501 catgaaaaat ttgcggacct caggaatcgc aaaattcttg tgctatgcgg gaaaggaaat
     4561 aacggcggtg atgggttggt ggctgccagg catctgaaaa attcgggatg cccggccgtt
     4621 gtttttctat ttgcgaatcc gggatcggtg gagggcgatg ctgcggctaa cttgaagcgc
     4681 tggcagcaag ggcttggtga aatgtacgtg gtgacgagcg aagcggaatg ggaatccgcg
     4741 cgcccggcgc tggtcgaggc ggaactcatt gtggacgcgc tgcttggcac gggattgcgc
     4801 ggccatgtag aaggtttact gggcaaagta atcgacgacc tgaacgcggc gcgtgcgaag
     4861 aggcgcgaca aaatggccgt ggtggccgtc gatatgccgt cgggacttgc ttccgattcg
     4921 caggattttg gcggtcccgt ggtccgcgcg gatttcaccg taactttgac tgcgccgaaa
     4981 gtcgggcaac ttgttttaca gaattcgacg cgctgcggag aactggtggt gaaagatatc
     5041 ggcacgccgc cggaactgct ggaatccgat ccgcacttga aaattaattg gatccaggct
     5101 ggggaattcc gcgcgctgcc gctggcgcgc gaacaagctt ccaataaagg aacgtatggg
     5161 cacgcgttaa ttgttgcggg atcgctgggg aaatccggcg cggcgattct tgcagggcgt
     5221 ggcgcgctgc gatcgggagc aggactcgtg acggctgcaa cgccgctcga tgtgctgcca
     5281 atcgtggctg gaggcatgcc ggaaatgatg acggcgcccc tggtttcgac cgacgcaggg
     5341 acagccagcc ttcgcaatct cgattacgga aaattttctg aaatattgcg cggcaaatct
     5401 gttctggcga ttggtccagg actttccatg cagcatgaaa ctcaacagtt tattcgccat
     5461 gtggtgggac agactgaaat tccaattatt ctggatgccg atggactgaa tgcattcgcg
     5521 ggcatgaagg acacgctgac cgaacgtaaa gcctcggcca tggttttaac gccgcatcct
     5581 ggcgaaatgg cgcgccttct gggcatcgcg gtgaaggaag tgcaactgcg gcgtttggat
     5641 gtagcgctcg aagctgctgg gcgctggcgt gcttacgtga tcctgaaagg tttccatacg
     5701 attcttgcta ctccatccgg gcatgcttac atcaacacga caggaaatcc gggaatggcg
     5761 acgggcggaa cgggcgacgt gctcacaggc atacttgcgg gcttgacggc acagtttggc
     5821 gtcgacgatt gggcgcgcgt gctgagcctt ggcgtgtact tacatggttt ggccggcgat
     5881 attgccgcgt cgcgcgtggg ggaatcgccg ctgattgcgt cggatgtgat cgacgcgatt
     5941 ccggaagcgt atgcgagagt gttggcggca gcgaagtaat atttggtcct gtagcacctc
     6001 gccttcaggc gggcatgcga tcgaactcca ggatgcctgc ctgaaggtag gcgctacaaa
     6061 actggattgc ctagcttggt ctaaagcatg atctataaaa tgcaaaatat gtctgagatc
     6121 ggaaattgga aatgtccgct gacgaaatca tcacgaattc ttacgaagaa acaatcgcta
     6181 agggtaggga aattgcctcg aaacttgttg ccccagtgct ggtcctgctg aagggtgacc
     6241 tgggtgcggg aaaaactacg ctgaccaagg gaattatcag tggcttggga gcggccaggg
     6301 aagaagacgt cacgagcccg acgttcaatc tggttcacga atttcgcatc gaacatccac
     6361 ttgcgcccgg ccagaacgcg cgcgcttgca aggtgtatca cgtcgatcta tatcgtataa
     6421 acagttttca cgatctcgaa tcgctgggca tcgaagatgc cctttccgaa aaagccattg
     6481 tgattatcga atggccggag cgattcacat ttcgcacgga ttggccaacg attgaaatac
     6541 atctcgacca tgcgggaggc gactcacgcc ggattgcgat ctcggatcaa acggtgaaga
     6601 aataatcgcc ggataaacaa tcgccgaata aacgatcgat atacttgaac ctgtatcaac
     6661 ttttttaatt gtcattgggt tcgctgcgag tatagccttc tacagaagta atccttccgc
     6721 taaatggaag agcgcgggtg aggggtttgt cgtgaagatc aggtgcgtat tctgcgttgt
     6781 aacgattgtg ctcggatctg tcgtcgggct cggttttaat tccagcgcaa acagtttgcc
     6841 tcaatcctca ttcccagcgc cttcgccgat taaatttgac catgccctga tttgcgtttc
     6901 cgatcttgct ccgatgcaac aggggcttac cgacgttggc ttgaaaccgg attacggcgg
     6961 gcatcacggg cacgcgacca ctcagatggc ccagcttgga tttccggatg gcagctacat
     7021 cggtgtggaa gcgccagtcg acgcgtcagt cgaaacggga accccgcaat cgaccctcat
     7081 gaaagcaaac gccggcccat gcgggtggtc ggccacttcg accgatatga agggcgattt
     7141 ggggagaatt tcgaaactgg gcgtcatagt tggagagccc gaagcgcgca gccgaactcg
     7201 tcctgacgga aagttggctg aatggcggag cgcatcgctg gggcctggcg cgcctgggtc
     7261 aaatctgccg ttcctgattg aagacaaggg accgcgcagc aaccgtgcgc cgcacgcttc
     7321 gcccagcaca gagggcacga ctctttccgg agtcggcgtg gtcgtgctgg gcgttaaaaa
     7381 tctggacgaa agcattgcgt tatttcgaaa agtgtacggc ttgccagcgc caaaaataga
     7441 ggagcatccc gaattcggcg cgaaggtcgc ctacttcgcg gggacgcccg tgatgctcgc
     7501 cgctgctgcc tctccagatt catgggtggc tgaacggatt gctcgactgg atcaaatccc
     7561 gcttgcattc ctgttacatg cggatgactt caaagctgct tcgaaacact tttctttgcc
     7621 cacagggaag ccttggttca atttagacgt atcctggttc gacgtccaaa agttacaggg
     7681 agtaagatta ggaatcgtcg gcggataagc ttcgaaagtg tcttcatcga ttttcagaag
     7741 cgcaggggaa attcagatga ccgaaattac acgacgtgga tttgtgaaag gggcagctgt
     7801 agctccatta gcattttcgc ctttcagcgt ggcgcaaaca tcttccgcgc ctcactacga
     7861 tgtggtgatt gtcggcgcag gtcacaatag cctaatcacg gcaagttatt tggcgaaagc
     7921 cggcttcaag tgcctcgtgc tcgaagggcg cccgatgatc ggcggaggga ccaagaccgc
     7981 gcaattgaca ttgagcggtt ttgagcacga tgtatgctcg tcgattcatg cccgaatcca
     8041 ggccaatccc ctgatgaaaa ataacgaatt gaatttgggt gaatacggcc tcgaatacat
     8101 ctttcccgat cctgtttatc acatcgcttt tccggatggc agttacatca cgaagtggag
     8161 agattttgac cgcacttgcg aggaaatcgc caaatattcc aagaaggacg cggaaacctg
     8221 gcggcgcatg gccaatgaag cggacgaaat ccggccactc gtaaatcaag tggaatacac
     8281 gcccatcgga ttcggaaaat cgcttgccga acgtttggcg gagcatccca aggggaaaat
     8341 ctggcagcgc cggatggcca tgtccaaatg ggatgtcctg cgccagaatt ttgaagatga
     8401 gcgatgatgc cgccattccc gaaggactta accgggccat attctggttt ctcggcatac
     8461 ccgagcaaga gagaagaagt tccgcagccg aagggcgggt cgggaatgat tgccacggct
     8521 ctgtcgcgtt tgctcgaatc gcacggctgc gaaattttgg tgaacaagcc tgttgagcaa
     8581 ctgatcgtag agaacggcaa gtgcgtgggc gtggaatgcg ccgacggaac ttcttatcgc
     8641 gcggacaagg ctgtgctctc cacggttcac atcaaacagc ttgtgcatat ggctccgaaa
     8701 gaaatgtggc cgcaggattt cctcgaaggc gtgaaaactt ttaacacagg aaatgcatcg
     8761 ttcaactctc attttgctac cactgaacct ttgaaattcc agaccaagag cggcacgatc
     8821 atgccggtcc acatttcaaa tattcagagc tgcaaacgcg cgctgcgctt cgaatctgag
     8881 ctaaccgccg gagagtttca cgtggaggaa cctgtgctgc acatcgtgca gacgagcgtc
     8941 gccgacccaa cgcgcgctcc ggccggcatg cacaccattc gcgttctttg cgaacaggcg
     9001 atttatgatt taccgaacgg cggccccggg cgctggtcgg agatcaaggc ggaagttcag
     9061 ttggcgcatc tcagggccgt ccagaagatt gctcccaatt tgacagatga taaaattctg
     9121 gcgcgcttct cgacttcgcc cgtagatatc gagcaaatga acgcggccat gtggcacggt
     9181 tcctgccata gcggcacgga cggtccggca caggctgggg ccatgcgccc ggtcccgggc
     9241 tggtcgcagc atcgcatgcc gatccccggc ctgtatcaaa cgggcggcac gacccatcct
     9301 ggaggtggcg tctcaggcgg ccccgggcga aatgccgccg tagtgatgct caaggatttc
     9361 ggcacgagca tggaagaagt gctaagcaaa aagggatagc cgggcgcaaa ccgccaacca
     9421 ggaatgccat agaagtttgt agcgtgcgcc ttcaggcgaa cacccgtgcc ccaccggcca
     9481 ctttatggag cgtgacttgt gaatcgtaac aaccgaaatt ttgggttgcc agtaatgttt
     9541 ggcttggctc tggcaattct ctctttcgga attacacaca tagccgctgc cgcacaatcc
     9601 gcgccctcac gggccgccag ccaggacgtg catgacaccc ctgtgccgct tcccgcgccg
     9661 gcgcaaccgc tcccttacag ccacaagcct catttggccc tgggtttaca atgcactttt
     9721 tgccacacga atcccggtgg agggaggcta atgacctttc cgcctacgtc tacctgtatg
     9781 agttgccatt cctctttcgg caaaaacagg cccagcattc aaaaattggc cgaatacgac
     9841 aagtcaaaga caccgattcc atgggtaaga gtctacgccg ttttgccggg cgttaaatgg
     9901 agccataaaa cccatttgga cgccggaatg aaatgtgaga tgtgtcacgg gaacgtcagc
     9961 cagatggtcg tgatgaccaa cgtgaagagc gtaaacacaa tggtcggctg cctggattgc
    10021 cacacattgc atagtgccaa gacgacttgt accacctgcc atcctgcctg ggcgcccgaa
    10081 atggcggtgg ataaacagaa gtaaggcgcc gggggaattg gactattgaa ggatgtttca
    10141 ttttggatga atggtttatg ttcccctgtc gatcattcgc aattcgaagg ctgcgcgcgc
    10201 tacacaaccc cgctgaaaac taaaaactta cgtcccgcca aaaatgtccg atctgctgtt
    10261 aacgggttgg attcgtcacc ttgttaggac attgccagct tgttaacctc gaatggagct
    10321 ttgcgaaacc gaatatgcct gaacgtacat ccatgctctc gcctgacaca cctgcccgga
    10381 tcaacctgaa ggcctattcc gctgagctat cactgcgttc tctggctgtg ggagtggaca
    10441 atatccacca cgacgttttc ctgagccccc tatttgcaga gacgacagaa gcctatctcc
    10501 ttgaatttat tcgccaaacg gtaaaccttc catttctttc gcagaaagat cgctcgccga
    10561 ccgagcgccg gacgagtccg agaagagaag cagataaaaa aaacgcgcgg tctcaagaag
    10621 ttgcgtcctg gaaaaagcaa ctctccgatc ttctgcattc gggtctccag aaggccaagt
    10681 tcgagaaaaa tatcgagatc gatcttcttc tccgcgccac tctgttgaaa tacctgacac
    10741 aggagatcgg cgcgcaattc gcaaatattc tgctggaagc gaaagaatgg attcggggac
    10801 gtggcgaaca ctttgatcgc agcgagcaag cgcacgtcat aaaggcctct ctggcggaat
    10861 tgcaggcgga tcgccgcaat cttttccgca aggtgggcca gcacgtatat caggcgatac
    10921 tcgatattga agacaacagt ctcgggagat cccggaaagc tttgtttggg gatgaggctt
    10981 ccgggtcata cgacatcttg aacaatcgac tggcctttgt cgaaaatggc aaagacgatg
    11041 tgctttttct cgagcaatac gtgctgctcg ggaattacat acgggatcaa gaccgctttg
    11101 aaaccttcga tacggtgatg ctggatctct tgcgggaatc tgtgttgaca ggggattccg
    11161 gaaacgattt gaatgagacg tggcagtcac atcaaaaact cgtggatgag gcagtggctt
    11221 atcaggcgga aatcgcacgc ctgaaagacg aacgcgagtc tctggtccgc aaactggaac
    11281 ggtccgaaag cctgatggga cgcgtaggat ttggttccga cccggcttcc atccgcgcat
    11341 cgatggcgga aatagaaaag cggcaacggt ttgcgcagcg caagctggaa gaaacaaatc
    11401 cgcggatcga agcttcgcac aatgaagcgg attatctggc caagcagtat caggagcgtc
    11461 tgggcgatta tctgaatcag ccggaaaatg cgcggagcct ttttgatcct gcagccacga
    11521 gcggagcgaa caaaggcagg gctggattgc gcgcccaact cttggaggaa ctcgttgtcc
    11581 ggctggagca gcgcgatttg ctggtgcaca ttctggccag ctaccagctg cggaacatct
    11641 gccacgatta ttgcccccca atccatctac agcaattgaa aaaagcgctg gtgtcacgcg
    11701 aagaattaaa gcgcgtggag gaaattctaa agcaatttcc cgcgcggcaa tattccgcgc
    11761 agcgcattga ggaactttcc aagaaaatcc gcaaaacgcc gcgcgaagaa gtgcgcgcgg
    11821 tggtcattcg cttcgcggaa gattttatgc ggttgcgccg tgacaccaga aattatgagc
    11881 ggctgagcgc tgcaatggag cgaattaatc tggtccgcaa cgaacgcagg cgggaacttt
    11941 cggagatgaa tcacagcctg tatgagtttc tgttgccgga ggaggcgcgg cccgcggaag
    12001 accatgtggt cacacacaca gtcatcaagg cggacgtgcg aggctcgaca aaaattacgc
    12061 aggatctgct ggagcgcggt cttaatccgg catctctctt cagcctaaat ttttatgagc
    12121 cggtgaagcg catcctcgat cggtatgggg cgtcgaaagt ttttatcgaa ggcgacgcgc
    12181 tggtgctggc gatttacgaa acggaatcca cgcgcgcgca acagcgcgct gtatcgaagg
    12241 cttgcgcgtt ggcccggcag atcctggctg tatccaccgc ttacaacgat cgccccgaca
    12301 cgaacgattt accgcgactg gagctgggag taggaattgc gtttcaggga tcggcgccaa
    12361 cttactggat ggatacggat tcacgtatca tgatttcgcg cgccttaaat ctttcggacc
    12421 gcctatcgag ctgctccaag gccgctaagc gcatgctggc gcaaaatcct tcgccatttc
    12481 ggctgttcct gtttcaaaca atgatggacg gcgtggctga ggatgaggct gacgagtttc
    12541 tggtccgcta caatttaaac ggcgtggaat taaacgagga aggttttcag aagctgcagg
    12601 aagaaatttc cttagcggaa ttggaagtcg actgcatcat gccgtggggg aaggaacgtg
    12661 tgacattttt ccgcggagaa acgccggtgg gagagggcct tgagcccgtg ctcattcgcc
    12721 gcggatttat tcgcgaaatg ctgccaggcg gaaaaattgg gcaggcgggg actcgaccgt
    12781 attacgaggt ttgcgtgagc ccgaaagttt tggagctagc ggagtttctc gcggagacgg
    12841 ttgcaaaccg gcgcgcctga actggtagga tgagggacag ggattgagcc gctgcagcca
    12901 attgcaaacg aatttcttga ttgtttttca gacttttcca tgagccgaag taaatcaaaa
    12961 tccaagccca gaaagttcac agctgctcgc gaggctcgcc ggcgcgcgcg tgaactggcc
    13021 ggtcctccac ctgccgcacg agtgatcccc gataaacgct tgaagccatc caagcacaag
    13081 aagaaattgg tggaagaata atcgggaccg ccttccgcct atctggcaac ccaacttttt
    13141 gtccagattt gtagcgcccg cctgcaggca ggcactgcgg attcaattac atgaccgcct
    13201 gaaggcgggt gctacatggg ccaatgcgag tgaccacaca gggcttgaag cctgccctgc
    13261 taatgaatgt gccgcgtttt tttctgcatg taatccatca gcagatattg gcccagggtg
    13321 tagggagtgg tgaagtacgc tttgccgcgg cagagttccg tcactttttg cacgaattgg
    13381 acgaggctat agtcgctggc caacataaac gtgttgatca gaattccgga ttttcggcag
    13441 cggcccactt cttccagcgt ttgcgtgacg acgagagggt caaggccaaa tgcgttttta
    13501 tagatgcggc cgtcttccag cgtgagcgcc gaaggtttcc catcggtgat catcacgatc
    13561 tgtttcatgt cttttttctg gcgcgcgagg atgcgccgcg cgaggcgcag accttcgcgc
    13621 gtattcgtgt agtaggggcc gacctggacg cgcgcgagtt ccgcgagcgg catttcttcc
    13681 gctgaatcgt ggaacaggac aagttgcagg gagtcgccgg gatattgcgt acgaatcagg
    13741 tgcgacagcg caagtgcaac ttttttcgct ggagtaaatc tatcttcacc atacaaaatc
    13801 atgctgtggc tgcaatcgag catgagcact gtggcacagg aactctgata ttccgtttga
    13861 tgcaccatca aatccggata atcgagttca attgggactt tcgatccctc acggcgcacg
    13921 gcattaaata atgtggcgct gatatccaga ttcagcgtgt cgccgaattc gtatgcgcga
    13981 gaaattccgc cggattcaac gcctgtcgaa agatcgcgtg tatcatggcg gccaaggctg
    14041 ctcttgccga gcgagcccat caaatccttg agcgtcttga atccgagaaa gtcgatggtt
    14101 ttatccgtaa tttcaaaacg cggctcattt tcgcggtcgc gttcgcggcc ctggcccact
    14161 tgtccgcgcg gagttttgtc aggtggcgcg gtaatttgcg gctgcccggt tacgtatcct
    14221 tcttccatca acttctcaat gagctggtcg atcaactcct tcatctttgc gtccatttgc
    14281 tcgggagtgc cgtcgagcaa atctttcatg tcctggtcac cgagcaagtc tccctgctcg
    14341 agggcgcgct gaattgcctg gcgcagggct tccatcgatt tttcggggtc catttcggag
    14401 aggccgtaga attgcgattc gaagccgctc tgaaggaaaa aatcagacag gcgtttgagc
    14461 aattcctgca gatccacgct gtccgcgggt tcgccggtat atttggtgta acggatgaat
    14521 tttgccatag cgtttactcg tttactggcg agggtgccga tctttacttt accccgcctc
    14581 tggcccttca cattctctca gggcaagaga gatgggctac aggaactttc ttaattaaat
    14641 tggcgacgcg gcgggcgctg ttgttgttcg tgcgggtccg gcactttggg gcgctcggca
    14701 tggaagccgc gctcttcgtt gcggctgatt cgcttgtgtg cccatagccc ttcgagaata
    14761 aattccgcgg ccgccgccat tccggctggc ccgcttcgcc cgtcgactcc caagcgctcc
    14821 acgttgtcca tcaggccctg gatcttttta agttgagcga gagcatccgc ggcgggagca
    14881 ttttcgggaa ttttcagttc gccgcccagc tcaaaccatt gcacgatggg ctgaagattg
    14941 gcgccctcca tgcggttagt aaaaaccttg cccacagcgt tgcgaattaa ttcacgcgcg
    15001 atgttttccg cgccgcggag ttcgccttcg tattcaagct ccatttttcc ggtgatggcc
    15061 ggaagcgcgg cataaacgtc cgcgacgcga gcagaggagc gcgattcctg attgcgcacg
    15121 gcacgctgct ccgcgctgct gacgacgctt tcgagcgcgg cgatgggcaa gcgctggctg
    15181 actccagaac gcttatcaat ccgtttatct tcgcgcgcga ggaatgccac gaattcgacg
    15241 acttcacgca aataattggg aacatcgacg gcggacctgc cgccatcgcg cgcgacccac
    15301 gcttcctgtt tcgtgatcga tacgccttct tcgacagtgg cagggtagtg cgtgcggatt
    15361 tccgagccga tacgatcctt gagcggcgta ataatttttc cgcgcgccgt gtagtcatcc
    15421 gggttggcag tgaaaacgag cagtacatcg agcggcatgc gcaccggata gccttttatc
    15481 tggatgtcgc cttcctgcat gatgttgaat aatcccacct gaatttttcc tgcgagatcg
    15541 ggaagttcgt tcacggcaaa gatgccccga ttggcgcggg gaagcaggcc gtagtgaatc
    15601 gtgagttcgt cgccaagggc acggcccgat cgcgcggcct tgattggatc gacgtcgccc
    15661 aaaatatccg cgatggttac gtcgggtgtc gccagctttt caatgaagcg cgcatcgcga
    15721 ccgagccatg agatgggcgt ggcatcgcct tgttccagga ctaaatcgcg gcacgctttg
    15781 caaatcggcg cgtagggatt gtcatgaatt tcgcaacccg tgatgtacgg catttccgta
    15841 tccaggaaat tgatcagtcc acgcagaatg cggcttttcg cctggccgcg caagccaagc
    15901 aagataaaat tgtggcgtgc aagcagggca ttggcaattt gcggcagaac ggtttcttca
    15961 tagccatgaa tgccgggaaa gagcggttcg ccagcttcga gctttttcag gatgttggcg
    16021 cggagttcat ctttcaccgt acggcgcgcg gttcctggcg catggctagg aaacgctttc
    16081 aattcaccca gagttccggg tttggttgtc atggggcccc caatcactca aaccgtatta
    16141 tatgtcagat ggaaaattac tgcagaattg aggaaaagcc gcaaaaaagc ccgcgcataa
    16201 ctgcaaattt gcccgtgtca ttggaaatat ggaaggtagg atcgcaagag gaggacatta
    16261 taacttgcta tggccattgc ctgctgcttt tcctcgaacg tgctcacgct tttttaactg
    16321 gggataccat tcctggagat gcgcgacgag ccgtgcctgt cgctccgatt cagagcgaat
    16381 ttcaaggagg ctttggcgca ttcccgtatc gagcggcagt tcggttgcga cgtggtaggc
    16441 gagcgaaatg cccccttcgg tttcaaaatg cggagcgtct tcgccataga gcaggccgtg
    16501 acactggttg cagaggtctt ccaactgctc ggaaacttca gaatccacgc cggtaaaatc
    16561 ttcctcaaga aataggatgt cggcttccat gtaagctttt tcttcgtgca tgcggattaa
    16621 gcggaacgcg ctttggccgg cggttagaat gtccgagcgg ccatcttcat attccttgag
    16681 cacttttacg atcagtgc
//
LOCUS       DASZMB010000076         5666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3637541, whole genome shotgun sequence.
ACCESSION   DASZMB010000076 DASZMB010000000
VERSION     DASZMB010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3637541"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1302
                     /locus_tag="VG272_03645"
     CDS             <1..1302
                     /locus_tag="VG272_03645"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWF12804.1"
                     /translation="QVISEAFYVASSGRPGPVLVDITKDAQQSTCEFDGKPIQPQLPG
                     YRPDLTPTQDEYREALELIKNSKRPMILAGHGIILSGAMDEVRTFAEKANIPVGLTLL
                     GLGAFPASHPLSLGMMGMHGEAWLNTTIQEADLLIAFGMRFDDRVTGNLKTYAPNAKK
                     IHIEIDRAEVNKNVKVDVALVGDLKGVLRELLPHIKPQEHREWLAYIDRLKGDSAVRD
                     IQNLPDNGHLYAAHVINDLWHETQNTDAVIVTDVGQHQMWEAQYYKHERPRTLITSGG
                     LGTMGFALPAAIGVKAARPEADVWVVVGDGGFQMTMCELATMVQENLKVNIAIINNGY
                     LGMIRQWQEFFYEKNYEASPILSPNFVKLADAFGIRSVAVTQRSEVVPTVKMAHEHKG
                     PVLIDFQVDKEDAVYPMVAAGAGLHEMIRRPNPLIETATDA"
     gene            1310..1885
                     /gene="ilvN"
                     /locus_tag="VG272_03650"
     CDS             1310..1885
                     /gene="ilvN"
                     /locus_tag="VG272_03650"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HWF12805.1"
                     /translation="MLNTFVVHVENKPGVLTRVASLFRRRAFNIDSLTVGRTEKPEVS
                     RMTIVVDADRDQALRVEANLYKLVNVLLVENITGKPSICRDLAIIKLSAKHNERSDIL
                     EIINVFRARVVDFAADSLTIEITGGEEKVDRLLEVLRPYGILEMVRTGIVAMRRGTKG
                     DEIAAATASATAGRANGAATVAADDNVSYSV"
     gene            complement(2029..2337)
                     /locus_tag="VG272_03655"
     CDS             complement(2029..2337)
                     /locus_tag="VG272_03655"
                     /inference="COORDINATES: protein motif:HMM:NF039244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5818 domain-containing protein"
                     /protein_id="HWF12806.1"
                     /translation="MKKILLSMAGLLMLLPVIPGIAVAAPSSRATQAQSEEKTRIFSG
                     TVIKDRDNFILSDAANKLSYILDNTREASKYEGKKVKVTGIVDAANNTIHVETIQEIA
                     "
     gene            complement(2594..3373)
                     /locus_tag="VG272_03660"
     CDS             complement(2594..3373)
                     /locus_tag="VG272_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWF12807.1"
                     /translation="MEPALVDLQNIQVVRGDNIALDNFSLRINSGEHVAILGPNGCGK
                     STLIKTITRECYPVIRDGSSMTILGHARWNVFELRSVLGIVSNDLMTDVTGETSGREV
                     VLSGFFSSTKIFDHLTVEPEHLKRADAALERIGVSHLADRPVEEMSSGEAKRILIARA
                     LVHDPKTLLFDEPSNSLDVYAQHGLREAMRELAQSGIGIVLVTHHLSDVIPEIDRVVL
                     IRKGKIQADGPKKDLLNTEEISRLFGIKLDITRRDGYYHLW"
     gene            3627..4385
                     /locus_tag="VG272_03665"
     CDS             3627..4385
                     /locus_tag="VG272_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HWF12808.1"
                     /translation="MNRGKILRGTWIAIIFLVVSGANAQEHRPAQILFESANRERAAN
                     GLRLLKWDVALAAAAHQHAQRIAAQNTLSHQLPGEPDMLARAKQARARFSSLAENVAE
                     GPSAENIHKQWMHSPPHRANLLDPQLNSIGIATVEHGGILFAVEDFSLAAQKLSVLEQ
                     ERIVAEKLTSSGLHLLNDEADARRSCTMDNGYAGSHTPSFVLHYATPDLQMLPDMLNQ
                     RIQTGKYHSAVVGACQSDAKLGFSSYRIAVLLFE"
     gene            complement(4410..4895)
                     /locus_tag="VG272_03670"
     CDS             complement(4410..4895)
                     /locus_tag="VG272_03670"
                     /inference="COORDINATES: protein motif:HMM:NF025734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HWF12809.1"
                     /translation="MKRVSAAKIVLVTIVVLVLIQFIRLSRTNPPVNPSRTLQAHVDV
                     PPEVLNVLKRSCYDCHSNSTVWPWYSNIAPVSWYVIRDVNVGRSHVNLQDWEAQINEQ
                     EGKEHLGLVCKLVREGKMPPADYRAVHKGTDVTPAETASICAWSQKVGTVEDDDKKED
                     H"
     gene            complement(4944..5498)
                     /locus_tag="VG272_03675"
     CDS             complement(4944..5498)
                     /locus_tag="VG272_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12810.1"
                     /translation="MRRFISRIVLSVGMFTLAASPIATGQPKLHNRLTWASLTLPLPE
                     QGPHGDHNPKHGGTFFMSMDYKHHLEGTFVSPGIFRIYLYDEHTKPLNETEMKRVRGS
                     IQIGETDTAPKIPLVPGSKKETLEADLSDRVRFPVTITLRLYLPGMESSAKPELFNFR
                     FAGFTDGRGSGNCRPMAGMPDMGC"
BASE COUNT         1308 a         1485 c         1578 g         1295 t
ORIGIN      
        1 caagtgatta gcgaagcgtt ttatgtggca agttcaggcc ggccaggtcc ggttctggtg
       61 gacatcacga aagatgcgca gcaatcgacc tgcgaatttg acggcaagcc catccagccg
      121 caactgccgg gataccggcc agatcttacg ccgacccagg acgaataccg ggaagcgctg
      181 gaactgataa aaaattccaa gcggcctatg atcctcgcgg gccacggaat cattttgtcc
      241 ggagccatgg acgaagttcg cacgttcgcc gagaaagcga atattccggt ggggcttacg
      301 ctgctggggc ttggagcatt tccggcgtcg catccattga gcctcggaat gatgggcatg
      361 cacggcgagg cgtggctgaa tacgacgatt caggaagccg atttgctgat tgcgtttgga
      421 atgcgctttg atgatcgcgt tacgggaaat ctaaaaacgt acgccccgaa cgcgaagaaa
      481 attcacattg aaattgatcg cgcggaagtg aataagaatg tgaaggtcga cgtcgcactg
      541 gttggcgatt tgaagggcgt tctccgtgaa ttgctgccgc acatcaagcc gcaagaacac
      601 cgcgagtggc tcgcctatat cgatcgcctg aagggcgact ccgcggtccg cgacattcag
      661 aacctgccgg acaacggtca cttgtatgcc gcgcacgtga tcaacgattt gtggcacgaa
      721 acgcagaaca cggatgccgt gatcgttacg gacgtgggcc agcaccagat gtgggaagcg
      781 caatactaca agcatgagcg gccgcgcacg ctgattacct ccggcgggct cggcacgatg
      841 ggatttgcgt tgcccgcggc aatcggcgtg aaagcggcgc gacccgaagc ggatgtttgg
      901 gtagtggtcg gcgacggcgg cttccagatg accatgtgcg agctagcgac gatggtgcag
      961 gaaaatctga aggtcaacat tgcgatcatc aacaacggct atctgggaat gattcggcag
     1021 tggcaggaat ttttctacga gaaaaattac gaagcttcgc ccattctcag tcccaacttt
     1081 gtaaagctgg cggatgcatt tgggattcgc tcagtggccg tgacgcagcg ctccgaagtt
     1141 gtgcccacgg tgaagatggc gcacgaacat aaagggccgg tgctgatcga cttccaggtg
     1201 gacaaggaag acgccgtcta cccgatggtg gctgcgggtg ccgggctgca cgaaatgatt
     1261 cgccgcccga atccgctgat tgaaaccgcg acggacgcgt gagagaaaaa tgctaaatac
     1321 atttgtggtg cacgttgaaa ataaaccggg tgtgttgacg cgtgtggcgt cgctgtttcg
     1381 ccgccgcgca ttcaacattg actcgctcac cgtcgggcgc acggaaaagc cggaagtttc
     1441 acgcatgacg atcgtcgtcg acgctgatcg cgaccaggcg ctgcgcgtag aggccaatct
     1501 ttataagctg gtcaatgtgc ttctcgtgga gaacatcacc ggaaagccgt cgatttgccg
     1561 cgacttggcg atcatcaagc tctcggcgaa gcacaatgag cggtcggaca ttcttgaaat
     1621 catcaatgtg tttcgtgcgc gcgtcgtgga ctttgcggcg gactcgctga ccattgaaat
     1681 tacgggcggc gaggaaaaag tagaccgctt gctcgaagtg ctgcgccctt acggaattct
     1741 agagatggtg cgcaccggta tcgtagccat gcggcgcgga acgaaaggcg acgaaatcgc
     1801 agcagcaacc gccagtgcaa cggccggaag agcaaacggc gcggcgacgg tagctgcgga
     1861 cgacaacgtt tcttactctg tttaacggac ctgtgccctt atatcggtag aaggtttcgc
     1921 acagagtcag agagaaaaga gaaaaaatgc ggagctcgag cgcggcctct gggggtttct
     1981 gctctctttc gtttctgatc tctagttttg agcacggtct taaattcgct acgcaatctc
     2041 ctgaattgtc tctacgtgga tcgtgttgtt cgccgcatcc acaattcccg tgaccttcac
     2101 ttttttccct tcgtatttgc tggcctctcg ggtgttatcc aagatgtagg acaatttgtt
     2161 cgccgcgtca ctcaaaatga aattgtcccg atccttgatc actgtcccgg aaaaaattcg
     2221 agttttttct tcggactgcg cctgagtcgc tcgcgaactt ggcgcggcga ccgcaatgcc
     2281 gggaataacg ggcagcagca tcagaagccc agccatggaa agcagaatct ttttcataaa
     2341 gcacccctcg cgtcgcgttc ggctggagtc aataaaggtg caagatgcat gccagaacga
     2401 cacatttcgt aacctgctgt attaacagag tttattttta tcggctggga tttgacgcga
     2461 attttttcct gttcgccgga agatataacc cggtccaccg gttcgagcta cacagcgaaa
     2521 ttattgtggc gcgccgtcgt gcaggcggat ttctcgatgt gacacgcgag aaaaagccgc
     2581 cggcagcaag gccctaccag agatggtaat agccatcgcg gcgggtaata tccagtttga
     2641 tcccaaacag gcgtgatatt tcctcggtat tcagcaaatc ctttttcgga ccatccgcct
     2701 gaatttttcc ttttcgaatg agcacgactc ggtcgatctc cggaatcacg tcagagagat
     2761 gatgcgtcac aaggacgatg ccgatacccg attgcgcgag ttcgcgcatg gcctcccgca
     2821 gcccgtgctg ggcatatacg tccagactgt tgctgggctc atcgaacaga agcgttttag
     2881 gatcatgcac cagagcgcgc gcaatgagga tgcgcttcgc ttcgccggag gacatctctt
     2941 ccacgggtcg atcggccaga tgcgagacgc cgatcctttc cagtgccgcg tctgcacgtt
     3001 tcaggtgttc gggttccacg gtcaggtggt cgaagatttt tgtgctgctg aaaaatccgg
     3061 agagcaccac ttcgcggccg ctggtttcgc ccgtcacgtc cgtcatcaag tcgttggaaa
     3121 cgatgccgag cacggaacgc agctcaaaaa cgttccagcg tgcatgacca agaattgtca
     3181 tagacgatcc gtcgcggatc actgggtagc attcgcgcgt gatggttttg atcagcgtcg
     3241 attttccgca gccgttcggg ccgaggatcg cgacatgctc accggaattg atgcgcaggc
     3301 tgaagttatc gagcgcaatg ttgtcgccgc gaacgacctg tatattttgc aaatcgacta
     3361 atgctggttc catgggctct ccaaagctct ccataatatc gaaattctct ctcgcccaca
     3421 cctacgaatc gagccgcatc gtgtttcgca gcgccggcat cttgccggca agttctcgct
     3481 ctctatggcg ataaaaaatg tcggcagggt gcgggcgcta cgaagttcga aaccgtcacg
     3541 ctttcgtcag ccgacgggga acggaagcca aactaatacg ttgtgagcgt aaaataatgg
     3601 ggcattctgc tcatttggga gccctcatga atcgtggaaa aattttacgc gggacttgga
     3661 tcgcgattat ttttttggtc gtcagtggcg cgaatgcgca ggagcaccgg ccagcgcaaa
     3721 tcctatttga atcggcgaac cgggagcggg ctgcgaacgg gctccgttta ttgaaatggg
     3781 acgtcgcgct cgctgcggcg gcgcaccagc acgcgcagcg tatagctgct cagaacaccc
     3841 tgtcgcacca gcttcccggc gagccggaca tgctggcgcg cgcgaaacaa gcccgcgcgc
     3901 gcttcagttc actggcggag aatgtggcgg aaggacccag cgcggaaaat attcacaaac
     3961 aatggatgca ttcaccgccg catcgagcca atctgcttga tccgcagctg aactcaatcg
     4021 ggattgcaac ggttgagcat gggggtattt tattcgcggt agaagatttt tcgctcgccg
     4081 ctcaaaaatt atcggtgcta gagcaggaaa gaattgttgc ggaaaagttg acgtcaagcg
     4141 gattgcattt gctcaacgat gaagccgacg cgcgtcgttc ctgcaccatg gataatggct
     4201 acgccggaag ccacactcca tctttcgttt tgcactatgc cacgccagat ctgcagatgc
     4261 tgccggatat gttgaatcag cggattcaaa caggaaaata tcattccgcc gtggttggtg
     4321 catgtcagag cgatgcaaaa ctcggatttt ccagctacag gattgcggtt ttgctttttg
     4381 aatgaacggc aggcttgaat atggctggct tagtggtctt ctttcttgtc gtcatcctcg
     4441 acggtcccaa ccttttgtga ccacgcacat atcgacgcag tctccgcagg cgtgacgtcg
     4501 gtccccttgt gcaccgcacg gtaatccgca ggtggcattt ttccttcgcg cacaagtttg
     4561 cagacgagac ccaggtgctc cttgccttcc tgctcgttga tctgtgcttc ccagtcctga
     4621 agatttacat ggctcctgcc cacatttacg tcgcggatta cataccagga gacgggagcg
     4681 atgttgctgt accacggcca aacagtggag ttggaatggc agtcgtagca agaacgcttc
     4741 agcacattta gtacttccgg cggaacgtcc acgtgggctt gtaacgtacg cgagggattt
     4801 acgggaggat tcgttcggct caaacgaatg aattgaatca ggacaagaac cacaatcgta
     4861 acgagaacaa tcttggcggc cgaaacacgt ttcatagaga ttttctcccg aaaattactg
     4921 aaaaatcggc atgcctgaat aacctagcaa cccatatccg gcatgcctgc cattggacgg
     4981 cagtttcctg atcctcggcc atccgtgaac ccagcgaacc tgaaattaaa caactccggc
     5041 ttggcactcg actccatgcc gggcaggtac agtcgcagtg taatggtcac gggaaatctt
     5101 actctatcgc ttaagtcggc ctccagcgtt tcctttttac tgccggggac gagtggaatt
     5161 ttaggtgcag tatccgtttc cccgatctga atggagccgc ggactcgctt catctcggtc
     5221 tcgttcaagg gctttgtgtg ttcatcataa aggtagattc gaaatattcc tggtgagaca
     5281 aaggttcctt ccaagtgatg cttgtaatcc atggacatga agaatgtccc gccatgcttt
     5341 ggattgtggt ctccatgagg cccttgctcc ggtaggggca atgtcaacga agcccatgtc
     5401 aggcgattgt gcagtttcgg ttggccggtt gcgataggtg aggcagcaag cgtgaacatg
     5461 ccaacgctca gaacgatacg gctaatgaat cgtcgcatcc aaaccctcct tgaatcttga
     5521 atattgaaat ccagttcgat gaaacgccca ttcattctcg cgctgcaccc cgacgaaatc
     5581 gttaccactg taccgaaaga gatgccccta gacttgtgac ttagatcacg cgtcaaaacc
     5641 gccattcgtg tttcgaatct gcagga
//
LOCUS       DASZMB010000077         9123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1187764, whole genome shotgun sequence.
ACCESSION   DASZMB010000077 DASZMB010000000
VERSION     DASZMB010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 9123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9123
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187764"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VG272_03680"
     CDS             complement(<1..801)
                     /locus_tag="VG272_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HWF12811.1"
                     /translation="MKLLMKSGIFLSLMLATLSLSSCYPAHSGSGGSLSSSGGSTGGS
                     GGTGGSGGSGGTATTFTIGGTVLGLTGTGLVLEDNAGDNLPIPANGTFTFKTAVSNAY
                     VVTILTQPTNPAQTCSISNGTGTATANVTNVLINCGTTGLTIGGSVSGLIGTGLVIQN
                     NGGNNFAVSGQGNVPFTFSAPVSGGTAYSVTVLKQPSNPPQVCSVVTGNGTATSNVTN
                     VEVICTQPGFKISGAVVGLVEGPGDTLELQNNAGDDLFVTGDTTFTFPT"
     gene            1343..4060
                     /locus_tag="VG272_03685"
     CDS             1343..4060
                     /locus_tag="VG272_03685"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HWF12812.1"
                     /translation="MRPSQIRNLIILAISVVVALWVSSTAADYIIEYNWWKEIGQVNT
                     WVGMLWYSVAPAAAGTVIAFIALWIAHARGLHFAGVLKRDHRIYSMLVPVGLAVLAMV
                     FASSAIDYWTVMRFFGSRGLTVSADAWKDQVFSRGLPFYLFDLPFYGQVLGFVFVLAI
                     LCALIFWATARGWQLFERFRYGRLDSGSHKVLTIEPGTLLLPGASRAGFVRVLTVILL
                     VGFTVWVYLGNYELLLNTHAFMTGADYVDEKVTLPLRWLLIVVSLGMIPFVWMRQYKK
                     AVAIVAAFFVLQIALPLVVHTVYVRPNEISIERPYIERHIAATSSAFGLNRNAEELPF
                     VPSAQAAVDPVQDATLLDNVRLWDLRAYNSTITQIQALRPYYTFPDTDVDRYFPNGRI
                     KQVLLSPREIDVTQLSEGASQSWINPRFIYTHGFGVVMSEVNKITPDGLPVLLIENAP
                     PEIKAPGFQVTRPEIYFGEKTQDPVFVHTAREEFDYPSGDQNKYSTYQGTGGFPVGSL
                     LLKTAAAISEGEPNIVFTSYLKGESRMMIHRNVQERLQYLAGFLDWEHDPYLVITDDG
                     RLVWMVDGYTTSQLHPYSATIPVPGLPDGVNYIRNSVKATVDAYNGKISLYVFDPTDP
                     IIQSYEHLFPKLFLPASEMPADLRRHTRYPEAIFQAQAEAYRTFHMRDPQVFYNKEDI
                     WDIAHNVAEQSNQAVNVQPTYVVATLPGEKTPEFLMILPFAPRGKDNLIGWMAARCDG
                     DHLGNLIFYQLPKQQLIYGPMQIESRIDQDQNISKDLTLWNQQGSHVLRGNIISLPVT
                     GGFLYVESIYIQANEARMPQLKKVVLAMGDRLIYRDTFEQALAELVGGGLPGKAAPGA
                     VVTAGQPAAPASPGNGAAAMADRVRQIRDQAEQLVKELEQLEKDAGAKK"
     gene            4162..4734
                     /locus_tag="VG272_03690"
     CDS             4162..4734
                     /locus_tag="VG272_03690"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWF12813.1"
                     /translation="MPNSQSLAGNFAAALPYDRYVQTGTEEQRRRWTQVYDAAHLTEA
                     QKELVAGFVREMKILIFSGIWCGDCVQQCPLICRIVDANPAKIDLRFIERQKENELPP
                     EWRINAGSRVPVVVFLSEDNEWCATAGDRTINRYRAIARAKLGPLCPTGIVAPDKDEI
                     DATLFDWLNEVERVQLMLRLTPRLRQKYQD"
     gene            complement(4834..6075)
                     /locus_tag="VG272_03695"
     CDS             complement(4834..6075)
                     /locus_tag="VG272_03695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF12814.1"
                     /translation="MAATQTETLVDAGNEVVFKVLGAISVCHLVNDMLASLLPAIYPL
                     LKTSFNLDFTQIGLITLTYQTTASLLQPLIGFYTDKNPKPYSLPIGMGATLVGLVLLS
                     IAHTFSALLVAAALVGTGSSVFHPESSRVARMASGGQHGLAQSLFQVGGNAGLSLGPL
                     LAAFVVMPRGQASIAWFTLAALLGMILLTGVSSWAKKRRLTWKKFSAKHVDKAPDLST
                     ARVTASIAILMALLFSKFVYLASLTSYYTFYLIGKFHMSVESAQVHLFVFLGAVAAGT
                     LVGGPVGDRIGRKRVIWVSILGVLPFTLLLPYSNLFWTEILSVVIGLILASAFSVIVV
                     YAQELVPGKVGMISGLCFGFAFGMAGLGAAVLGWLADLTSVNFVYLVCSYLPAMGLLA
                     AFLPDIERPAKIAIHEDALSE"
     gene            complement(6182..7069)
                     /locus_tag="VG272_03700"
     CDS             complement(6182..7069)
                     /locus_tag="VG272_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HWF12815.1"
                     /translation="MLLTPMAIAASGAIAGAAGAKKMTLAMHQNTSNAAGYRKSLEGW
                     ARAGIKDVEITNVLLDEFLKTEDLAAAKRVISDLGLNLVHAATGVIELWEPNPNRETA
                     LDHLKKRCEMYANMGLHHVYSPTTCTRKVTEDDYKAAPDNMFEAGEIAKQFDTKLRIE
                     FMRASTFISTLPTLLKLTREAAHPNIEPMLDCYHFWSGLDKFEDLDMIRNGEIGHVHF
                     QDVPDIPREMLDLSTRIIPGDGISPLTRILGKLAEKGYAGPLSVELFLPKFQQGDPYE
                     IAREVRTKSEPVMRQAGVA"
     gene            complement(7195..8088)
                     /locus_tag="VG272_03705"
     CDS             complement(7195..8088)
                     /locus_tag="VG272_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12816.1"
                     /translation="MKTVDVRENRGRRTFLKAAGGAAVGALAAAKDSMAQTENSNGSM
                     QMSRMKMAPPDRGLWITWYDLPAEERDAYFSWLHGTYLPQLLKRPGYLWAGHYASQDL
                     EGGADNAKRYKHIDDPKVGKGFHYMLLVAATDANVFGDPVPSVINAALPEKDKKMLAM
                     RMGERMNIFTETGRRDGTAHAAHKEGMLGTPCIQVGSFDCPTEFEEEMHAGYVLERLP
                     KMCATPSCVRVRKLNSVAGWAKHGILYEFASKEGFDRDYAPAVAHSPLGLNGHSVVPE
                     LMHAPNGPNSALRIWPPVAKT"
     gene            complement(8111..>9123)
                     /locus_tag="VG272_03710"
     CDS             complement(8111..>9123)
                     /locus_tag="VG272_03710"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF12817.1"
                     /translation="AGRGRRVEIGGKRTPVVDIHAHVRVPEAWDLLKDRIVSEGRAGD
                     AQLGNPDNSANIHSDVQKHLADLDEMGIDVQALSINPFWYWADVELSRKIVQIQNEKL
                     AELCAAHPARFVGLGSVALQHPVLAAEQMEYGVKHLGMRGVAIGGSVNGDDLSATKFH
                     PFWAKAEELQTLVFIHPQSAGAPIDEKRLQGNGFLDNVIGHPLETTLALTHLIEDGTL
                     DLFPRLKICAAHGGGYLPSYSSRSDQCLTAFPKLCKPLKKPPSEYLKQLYFDSVLFSA
                     EDMRHLIAVVGASQVVVGTDYPTLWNRTPVDRILAVPGLNDAQRISIFSSTAAKLLKI
                     AI"
BASE COUNT         2059 a         2642 c         2349 g         2073 t
ORIGIN      
        1 cgtagggaat gtgaatgttg tatcgcccgt tacaaacaga tcgtcgcccg cattattttg
       61 caattccagt gtatcgccgg gcccctcgac caagccaacc acagctccgc tgatcttgaa
      121 accaggctga gtgcaaatga cttccacatt ggtcacattc gaggtggcgg tgccgttgcc
      181 agtgactacg gagcagactt gcggcgggtt cgatggctgt ttcaaaacag tgacgctata
      241 cgctgtgcct ccgctgaccg gggccgaaaa tgtaaaggga acgtttccct ggccggagac
      301 tgcgaaattg ttgccaccgt tgttttgaat aacgagcccg gttccgatca acccggaaac
      361 cgaaccgccg atcgtgaggc ccgtggtgcc gcaattgatc aacacgttgg ttacgttggc
      421 cgtggcggtc cccgttccgt tcgagatgct gcaggtctgc gccgggttcg tgggctgcgt
      481 caaaatcgtc actacgtatg cgttgctgac cgccgtctta aacgtaaagg tgccgttcgc
      541 cggaatcggc aggttatctc cagcgttatc ttccaggacg aggcccgttc ccgtgaggcc
      601 gaggacagtt ccgccaatcg taaaggtcgt agctgtaccg ccactacccc cgctgccgcc
      661 ggtgccgccg cttcctcccg tgctaccgcc tgaactactg agcgaaccgc ccgaacccga
      721 gtgtgccgga tagcaggaac ttagggacag cgtcgcgagc ataagcgaga gaaaaatacc
      781 ggacttcatc aaaagtttca tggctgcgga gtgctccttg atggcgaacg aggaacgcac
      841 taccccatcg caatagtgcg gcgctgggga tccaccctca gtggaagcga acctatacct
      901 gtgtttccga cgttcgcgga attacccgat aaccaatacg tcaatattta gctggctgtg
      961 tacgtctacc taaacaccct aattcgagtc accattaggg tcaattctga cgttgaggtg
     1021 atgtgcctac ctgaataatg aacagaagga gttaaataga aaatatgtga gtaaaataat
     1081 ttggggagtg ccttgcgctt caaaggtcat ttaaaattaa tgacttactg atactccccc
     1141 aatagccatc ccgtgtggaa aattagtgtc ctcttgtcgt tttggaaatc ctcccccgat
     1201 atttcttggc gtttctcgcg cgctcgttcc acctcctgca tgtgatagta tctaaaccgg
     1261 tttacataaa acttaccgcg ctgcttcccc gcgatactac aattcgggcc tgtggcactg
     1321 tttgcaggtt ggaactgccc ttatgcgtcc atctcaaatt cgaaacctga ttattctcgc
     1381 aatctcagtc gttgtcgcac tttgggttag ttccacggcc gccgactata ttattgaata
     1441 caattggtgg aaagaaatcg ggcaggtcaa cacatgggtg ggaatgctgt ggtatagcgt
     1501 cgcacccgcc gcggccggaa ccgtaattgc gttcattgca ctgtggatcg cgcacgcgcg
     1561 cggcttgcac ttcgcaggcg tgttaaagcg tgaccatcgc atctattcca tgctagttcc
     1621 tgtcggcctg gccgtgcttg cgatggtttt cgcttcgagc gcaattgatt actggaccgt
     1681 gatgcgcttt tttggctcgc gcgggctaac cgtttccgcc gatgcatgga aagatcaggt
     1741 tttttcccgt ggcttgcctt tctatctctt cgatttgccg ttttatggcc aggtacttgg
     1801 cttcgtattt gttctggcga ttctttgtgc gctgattttt tgggctactg cgcgtggttg
     1861 gcagctgttc gagcgtttta gatacggaag gttggattcg ggctcgcaca aagttctaac
     1921 gattgagccc ggcaccttac ttttgcctgg agcaagccgc gccggtttcg tgcgtgttct
     1981 cacagtgatt ctacttgtgg gctttaccgt gtgggtttat ctgggcaatt atgaattgct
     2041 gctgaacacg catgccttca tgacaggcgc ggattacgtc gacgaaaaag ttacgcttcc
     2101 tttacgctgg ctactgatcg tcgtttcgct tggaatgatt cccttcgtgt ggatgcgcca
     2161 atacaaaaag gctgtagcga ttgtcgccgc gttctttgtt ctgcagatcg ctttgccctt
     2221 ggtcgtgcac accgtgtacg tgcggcccaa tgaaatttcc atcgagcgcc cctatattga
     2281 gcgccatatt gccgccacat ccagcgcctt cggattgaat cgtaacgcgg aagaactccc
     2341 gttcgtgcca tccgcccaag ccgcggttga tcctgtgcag gacgcgacgc tgctggacaa
     2401 cgtccgcctg tgggatttgc gcgcttataa ttcgacgatt acgcaaattc aggcactgcg
     2461 cccttattac actttccctg acacggacgt ggatcgctac ttccccaatg gtcgcatcaa
     2521 gcaagttttg ctgtcgccgc gcgaaatcga tgtgactcag ttatcggaag gcgccagcca
     2581 gagctggatc aatccgcgct tcatttacac tcacgggttc ggcgtggtca tgtctgaggt
     2641 caataaaatt acgcccgacg gccttcccgt cctgctaatc gaaaatgcgc cgcccgaaat
     2701 taaagctccc ggatttcagg tcacccggcc tgaaatttat ttcggtgaaa agacgcagga
     2761 tccggttttc gtgcacacgg cgcgcgaaga attcgattat ccgtccggcg atcagaataa
     2821 atattccacg tatcagggca cgggaggatt tccagtggga tcgctcttgc tgaaaaccgc
     2881 ggcggcgatt tccgaaggcg aacccaacat tgtttttacg agttacttaa agggcgaaag
     2941 ccgcatgatg attcaccgca acgtgcagga acgcttgcag taccttgcgg gatttcttga
     3001 ctgggaacac gatccgtacc tcgtcatcac ggacgacggc cgcctcgttt ggatggtgga
     3061 cggctacacc acttctcaat tgcatccgta ctccgccaca attccagtgc ctggattgcc
     3121 cgacggcgta aattacatac gcaactccgt gaaggccacg gtagatgcat acaacggaaa
     3181 aatatcgctg tacgtctttg accctaccga tccgatcatt cagtcgtacg agcatctctt
     3241 cccgaagttg ttcctgcctg cctcggaaat gcctgcggat ctgcgccgcc acacgcgcta
     3301 tcccgaagcg attttccagg cgcaggcaga agcgtatcga acctttcata tgcgcgaccc
     3361 gcaggttttc tacaataagg aagacatctg ggacatcgcg cacaacgtcg cggagcagtc
     3421 taaccaggca gtaaatgttc agcccaccta cgtagtcgcg acgctccccg gggaaaaaac
     3481 tccggagttt ctgatgattc tgccgttcgc gccgcgcgga aaagacaatc tgatcggatg
     3541 gatggccgcg cgctgcgacg gcgatcacct gggaaacctg atcttctacc aattgccgaa
     3601 gcagcagttg atatacggtc caatgcaaat cgaatcgcgc attgatcagg accagaatat
     3661 ttccaaggac ctgacgcttt ggaatcagca gggctcacac gtcttgcgag gaaatattat
     3721 ttcacttccc gtgaccggcg gatttttgta cgtcgagtcc atttacatac aggcgaacga
     3781 ggcgcgcatg ccgcaactga aaaaagtcgt gctggccatg ggcgatcgcc tgatctatcg
     3841 cgacacgttc gagcaggctc tcgccgaact ggtcggtggc ggcttgccgg gcaaagccgc
     3901 tcccggcgcc gtcgtgacag caggccaacc tgcggcgccc gcttcgccag gaaacggcgc
     3961 ggcagccatg gccgaccgcg tccgccagat ccgcgaccaa gccgaacagc tagtaaaaga
     4021 gttggagcaa ttagagaaag atgcaggtgc caaaaagtag aacaggcttc agcctgtttt
     4081 cttttgactt ttttaagcat ctaacaaatt caaaccaaga caggctgaag cccgtcctac
     4141 gaaatgcgaa atccacaaac tatgccaaac tcacaaagcc tggccggaaa tttcgcagca
     4201 gcccttccct acgaccgcta tgtccaaacc ggcacggaag aacaacgtcg ccgctggacg
     4261 caagtctacg acgctgcgca cctgaccgaa gcccagaaag aactcgtcgc cggtttcgtg
     4321 cgcgaaatga aaatcctgat cttcagcggc atctggtgcg gcgattgcgt ccagcagtgc
     4381 cccttgattt gccgcatcgt cgacgcgaat cccgcaaaaa tcgatctccg atttatcgag
     4441 cgccaaaagg aaaacgagtt accccccgaa tggcgcatca acgccggcag ccgcgtcccc
     4501 gtcgttgttt ttttatccga ggacaacgag tggtgcgcca cagctggcga ccgcacgatc
     4561 aaccgttacc gcgccatcgc tcgcgcgaag ctgggacccc tgtgccccac aggaattgtc
     4621 gcccccgaca aagatgaaat cgacgcaaca ctgtttgatt ggttgaacga agtggaacgg
     4681 gtgcagctga tgcttcgact cacaccacgc ctccgacaaa aatatcagga ttgataggcc
     4741 tcggcagacc aggtagcaca ggcttcagcc tgtggttttt gggccttgca agaaccaaaa
     4801 tccacagcct gaagcctgtg ctactaagaa ccactactcc gacaaggcgt cttcgtgaat
     4861 cgcgatcttc gcgggccgct cgatatccgg caaaaaagcc gcgagcaaac ccatagctgg
     4921 caggtaggaa caaacgaggt acacaaaatt aacgctggtc aaatccgcga gccagcccag
     4981 cactgccgcg ccgagccccg ccattccaaa cgcaaatcca aaacacagcc cggaaatcat
     5041 tcccactttc ccaggcacaa gctcctgcgc gtacaccacg atcacggaaa acgccgaagc
     5101 cagaatcagg ccgatcacca cgctcaggat ttccgtccag aataaattcg agtacggcag
     5161 cagcagggtg aacggcagca cgccgagaat cgaaacccaa atcacgcgct tgcgcccgat
     5221 ccgatcaccg accggtcctc caaccagcgt gcccgcggcg accgcgccca gaaaaacaaa
     5281 taaatgaacc tgcgcgcttt ccacggacat gtgaaatttg ccgatcaaat aaaatgtgta
     5341 gtaactcgtg aggctagcca ggtaaacgaa tttggaaaat aacagcgcca ttaaaatagc
     5401 gatggaagcc gtgacgcgcg cggtggatag gtccggcgct ttatcgacgt gcttggcgct
     5461 gaattttttc cacgtcaacc ggcgcttctt ggcccaacta cttacgcccg tcagcagaat
     5521 catccccagc agcgcggcga gcgtgaacca cgcaatgctg gcctgacccc gcggcatcac
     5581 aacgaacgcc gcaagcaacg gtccgagcga gagcccggcg tttccaccca cctgaaagag
     5641 cgattgcgcc agaccgtgct gccctcccga tgccatgcgc gcgacgcgcg aagattccgg
     5701 atgaaacaca gaagaacctg ttcccacgag cgccgccgca acgagcagcg cggaaaatgt
     5761 gtgcgcaatc gacagcaaca caagccctac cagcgtcgcc cccattccga tgggcagcga
     5821 gtacggcttg ggatttttgt ctgtgtaaaa accaatcaaa ggttgcagca gcgatgcggt
     5881 ggtctgataa gtcagcgtga tcagtccgat ttgtgtgaaa tccaaattga aagaagtttt
     5941 gagcagtgga taaatcgcgg gcagcagcga cgccagcata tcgttaacaa gatggcagac
     6001 gctaatggcg ccgagcactt tgaaaacgac ttcattccct gcgtcaacta aagtctcagt
     6061 ttgggttgct gccaagtttc ttttccctct acatccagaa ttaacacaga agtgaaacag
     6121 attttgtcta ttctagcagg cgatggcaaa gttcataggt cagacacgcc agatggggcg
     6181 ctcatgccac gcccgcctgg cgcatcaccg gttcggattt cgtgcgaacc tcgcgtgcaa
     6241 tttcgtacgg gtcgccctgc tggaattttg gcaaaaataa ttccaccgac agcggccccg
     6301 catatccctt ttccgcaagt tttcccagga ttcgcgttag cggactgatt ccatctccgg
     6361 gaatgattcg agtagagaga tccagcattt ctcgcggaat atccggcaca tcctgaaaat
     6421 ggacatgacc gatttcgccg ttgcgaatca tgtccaggtc ctcgaatttg tcgaggccgg
     6481 accagaaatg ataacaatcg agcatgggct cgatattcgg atgggcagcc tcacgcgtca
     6541 gcttgagcag tgtgggcagc gtggaaataa acgtcgaggc tcgcatgaat tcaattcgca
     6601 gcttcgtatc gaattgcttg gctatctccc cggcttcaaa catgttgtcc ggggcggcct
     6661 tgtaatcatc ttccgtaact ttccgggtgc aagtcgtcgg cgagtacaca tgatgcaggc
     6721 ccatattcgc gtacatttcg cagcgttttt tcaaatggtc gagcgcagtt tcgcgatttg
     6781 gattcggctc ccacagctca atcacgcctg tcgcggcgtg cacaagattc agtcccaggt
     6841 cagagatcac gcgcttggcc gccgccagat cttccgtttt tagaaattca tccagcaaaa
     6901 cgttggtgat ttcgacatcc ttgattcccg cgcgcgccca cccttctagc gatttacgat
     6961 atcccgccgc gttcgacgtg ttctggtgca tggcgagcgt catttttttg gcacccgcgg
     7021 ctcccgcgat ggcgccgctc gccgcaatcg ccatcggtgt caacagcatt tctctcctgg
     7081 aaatcgtgtc catcgaacgt cctttttttt aatccttctg tagcccgact cttccgagtc
     7141 ggactgcaaa agatgcattc tcaatcctcg gcggggaaca gattccgcga tgcgttacgt
     7201 cttcgccacc ggcggccata tccgcagcgc actgttcggc ccgttaggcg catgcatcaa
     7261 ttccggaacc acggagtgcc cattcaatcc taacggcgaa tgcgcaaccg ctggcgcgta
     7321 gtcccgatcg aaaccttcct tcgatgcgaa ctcatacaga ataccgtgct tcgcccatcc
     7381 ggccaccgaa ttcagtttac gcacgcgaac gcacgagggc gtcgcgcaca ttttcggcag
     7441 acgttccagg acataccccg cgtgcatttc ttcttcgaat tctgtcgggc aatcgaagct
     7501 gcccacctga atgcagggcg ttcccaacat tccctcttta tgcgcagcgt gtgccgtgcc
     7561 gtcgcgccgc cccgtctccg taaaaatatt catgcgctcg cccatgcgca tcgccagcat
     7621 ttttttatct ttttcgggca gcgcggcgtt aatcacgctg ggaacgggat cgccaaacac
     7681 attggcgtca gttgcggcca cgagcagcat gtaatgaaaa cctttgccga ccttcggatc
     7741 gtcgatatgt ttataccgct tggcgttatc cgcgccgcct tcgagatcct gcgacgcgta
     7801 gtgccccgcc cacaaataac cgggccgttt taatagctgc ggaagatacg tgccatgcag
     7861 ccatgagaaa taggcgtcgc gttcctccgc cggcaaatcg taccaggtga tccacaatcc
     7921 tcgatccggc ggcgccatct tcatccggct catctgcatc gagccatttg aattttcagt
     7981 ttgcgccatc gaatcctttg cggcggccaa tgcgcccact gccgcgcctc cggcagcctt
     8041 caaaaatgtg cgtcggcccc ggttttcccg aacgtcaact gtcttcattg cgtctcctcg
     8101 ttcatcgaat tcaaatcgcg attttgagca atttggccgc cgtactgctg aaaattgaaa
     8161 tgcgttgtgc gtcattcaat ccgggaaccg ccagaattcg gtccacagga gtgcggttcc
     8221 acagcgtagg ataatccgtc cccacgacaa cctgactcgc gccaacgaca gcaatcaggt
     8281 gcctcatgtc ttccgcgctg aatagcacgg aatcaaagta aagttgcttc aagtattcgc
     8341 tcggaggttt tttcaagggc ttgcaaagtt tgggaaacgc ggtgaggcat tgatcgctcc
     8401 ggctggaata ggaaggcaaa tagcctccgc cgtgcgccgc gcaaattttt aaccgcggaa
     8461 aaagatccag cgtaccgtct tcaatcaaat gcgtgagcgc gagcgtggtt tccagcggat
     8521 gcccgataac attgtccaga aatccatttc cctgcaagcg cttttcgtcg atcggcgcgc
     8581 ctgccgattg cgggtgaatg aaaacgagtg tctggagttc ctcggctttg gcccagaacg
     8641 gatgaaattt cgtcgcggaa agatcgtcgc cattcacgct gccgccaatg gccactcccc
     8701 gcatccccag atgcttcacg ccatattcca tctgctccgc ggcaaggacg ggatgctgca
     8761 aggctacgct gccaagtccg acaaagcgcg cgggatgtgc ggcgcaaagt tccgctagtt
     8821 tttcgttttg aatttgaaca atctttcggg acagctcgac gtccgcccaa taccagaacg
     8881 ggttgatgga aagcgcctgc acatcgattc ccatttcatc caaatccgct agatgctttt
     8941 gaacgtcact gtgtatattc gccgaattgt ccggatttcc cagctgcgcg tcgcctgcgc
     9001 ggccttcact gacgatgcga tccttcaaga ggtcccaggc ttcgggtacg cgaacgtgag
     9061 cgtgaatatc gactaccggc gttcgttttc cgccgatctc aactcgtcgt ccacggccag
     9121 cgg
//
LOCUS       DASZMB010000078         9923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1997391, whole genome shotgun sequence.
ACCESSION   DASZMB010000078 DASZMB010000000
VERSION     DASZMB010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 9923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9923
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1997391"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2270)
                     /locus_tag="VG272_03715"
     CDS             complement(<1..2270)
                     /locus_tag="VG272_03715"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HWF12818.1"
                     /translation="MNSVKPGIGRIARIGSKNDVPLLVCLLSVVLYCLPAFSQGSFGR
                     ILGTVTDQSGGVISGATITVIDTERGISRTLMTDDAGAYNAPNLTAGNYTVRGEAKGF
                     KKIERQAVLIEVGHEVRVDLVVQPGEQNQTVTVTEAVPLVETTNATMGGTLENADIID
                     LPLNGRDYQNLLGLRPGVMLQPGGGPWTQSTNGARPDESVWLIEGIINSNPFDARPVI
                     NMPSPFTDGATILPVDAIQEFNLQENPKAEYGWKSGAIVNVGIKSGTNTLHGDAYAFG
                     RYQAWDARNYFNIASPVGCSAALLQSNGQCPQTDAQLKQFGGVVGGPIKKDKLFFFGG
                     YEGLRSLIGFVGGIPVPNLVTVGNPALSMVDAITALQGKGIPISNVSQKLSGCNATAT
                     PVICTGVDAGSQLFPNTGTSNTFLSTFPTQNTSDNGVGKLDFHANDKNSFNGMFFYGH
                     YTATGEDHSFVSQNATDSTPITAMTITSSWVYTPNSNIVNELRFGYNRVSFSFINVDV
                     NSPSSTYGISTGVTNPLAGGLPSIVINGFGNGGTPVVGTAFNRPQYFTPNPYWDLQDS
                     VSVLKGKHSIKVGGEFTHLEADAAVFNNGRGRFNFFGNGAFAGSTPLEDFFAGTLSPP
                     PGGSAATLLSGTPQSKLICYNYAAYIQDDWRVTPRLIVNAGLRYNLNTPMKDAFGNIG
                     NFDPSSPTGMVQQGQPGWDTIWKTDPFDFEPRLGMAWDMKGNGTTVVRLGVGLIHETW
                     NLETFEGQFNTQGDGST"
     gene            complement(2809..3453)
                     /locus_tag="VG272_03720"
     CDS             complement(2809..3453)
                     /locus_tag="VG272_03720"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HWF12819.1"
                     /translation="MPEFELPRSIAIEGPIRVGKSTLSRILADRLHARRVFDPDDNPF
                     LTDFYKEKPGSAFRAQMYFLIERHQRWLEAQKNDASPLVSDFLFEKDKIFAYVNLDNE
                     ELKVYERYFGMFAATLKPPDLVIYLQATPEVLRERIARKKSPAENQISRAYLEEIVRA
                     YEHFFFHYSASDLLVINTSEIDFVERNEDLQQLLRRLQQPVKGTQYFLPLGASD"
     gene            complement(3554..5377)
                     /locus_tag="VG272_03725"
     CDS             complement(3554..5377)
                     /locus_tag="VG272_03725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWF12820.1"
                     /translation="MKLALRSYWHVAAAWFLVVGVYIAICEFVPIGVGRTTASNLLFC
                     LIPLFVNGALLLNAVTPDWRKRTFWMLLAFGCVLWMSGQILWTYAVVYQHRPVLNLFG
                     GDIIFFLHTVPMIAALTMQPHKRSDDRNILYDYVDFGLLLCWWLYVYVFAVIPWLIVA
                     ANVRNYIHAHEIVTALENLAFAAGAAFLAAKTTGYWRKIYIHLAGVGAIYTFGLVTIN
                     FGIDRAAYSMSELYNVPLLISFLWLATVGIIARQTAEPTTKAAAEKSGELAAGDASQH
                     GSAWTANLARMALLSLPLIGFWCMKGSVAPAAVRDFRILVTLGAALPLGFLAFLRHDL
                     VNRERLRLLRASHESIDNLKRLQTQFVQSEKLASIGQLVGGAAHEINNPLTAILGYSE
                     LLADDPMIGEKTRGIAEKIREQARRTRGLVNNLLSFARQAPSEQRATLDVNTIVTTSA
                     QFRRFDLRGQNIRIEVQTSAGIPEVRADMNQLLQVFTNIINNAVYAMQESGGGVLTVR
                     TMFEKGNVVILFSDTGPGMRDPNLVFDPFYTTKPVGKGTGLGLSICYGLVRDQGGQIS
                     CYNRPEGGATFRIELPAVPTAFPFAPRSVGAQARSNPAKLA"
     gene            complement(5683..6936)
                     /locus_tag="VG272_03730"
     CDS             complement(5683..6936)
                     /locus_tag="VG272_03730"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWF12821.1"
                     /translation="MNREARKLMRDAVFLFTATLAFGTADWAAPQAGTPAGIPAKAST
                     AKQATNAKLPPLEIAREGYLFAGGSYATVKDAKVMVNQIYAEFQIPVHRKSPYPIIMV
                     HGGSQSGTNFTGTPDGREGWAQFFLRHGYAVYVVDQVGRGRSNYQDEMYGPNMPADLV
                     ATQKRFTNVERYNLWPQAHLHTQWPGAGVPGDPIFDQFFASQLPSIANYALQQGLNRD
                     GILALLEKIGPAILLTHSQSGAFGWPVGDARPDLVKAIVAIEPNGPPFFNVDNTGAPD
                     WFREVPENVRPWGPTPDALTYDPPVSSPSDLAIVRREKPDDPNESSCWMQISPARQLP
                     KLQKIPILVITSEASYHRAYDHCTVEYLEQAGVPVKWVKLADVGIHGNGHMMMLEKNN
                     MEIAGVMAQWIEKTVPSQMDTSAKK"
     gene            7077..8576
                     /locus_tag="VG272_03735"
     CDS             7077..8576
                     /locus_tag="VG272_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWF12822.1"
                     /translation="MAESIAQEKLRFEKADADFLTRFPEYRNTSLLDDFRATEYRRLE
                     EFRHVYLDYTGGSLWAESQIQKHFDLLRSSVLGNPHSASPASVAMTDHVERTRRHVLR
                     FFNASAEEYILVFTQNATAALKLIGESFPFAAGSRYLLTFDNHNSVNGIREFARVKGA
                     TIEYAPLAMPDLRLDAGKLDAVLDGADPRHANLFAYPAQSNFSGVKHPLDLVAKAQSK
                     GWRVLLDAAAFVPTSRLDLSDTQPDFVTLSFYKMFGYPTGVGALLIRRSALAALKRPW
                     FAGGTVNFASVQGQAHVLSPNEAAFEDGTLNYLSIPAVEIGLQYLESIGIDIISERVR
                     CLTGWLLEELLALRHTNGRAMVRIYGPATTQARGGTVTLNLYDPHGHLLDYRRVEELA
                     GAENISLRTGCFCNPGAGEIAEGLTEEDMRAGFTMGAEINLLSFVRMMQERGHKSAGA
                     IRASIGVASNFADVWRFLRFVAGFRDQTRLTIGEVSFDIESCRIIRDGS"
     gene            complement(8663..>9923)
                     /locus_tag="VG272_03740"
     CDS             complement(8663..>9923)
                     /locus_tag="VG272_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HWF12823.1"
                     /translation="EMHPKKKRTWKNNITLHVFIAIVAAIILGEVDPKLAVQMRPLGD
                     LFIKLIRMAIAPIVFLCVSTGVAHIGDVRKVGRIGLKALIYFELVTTLALAIGLASVV
                     IFKPGVGVNAQQTGATSTDYSAYAGSGKNFTVLGFLLGTVPDNFLGSFVRGDLLQVVV
                     IALLFGMAMIGMGERGKAVVHSLDGIMMAFFGIIRIIMRVAPIGAFGAMAFTVGKFGN
                     GALFALSKLIVCTYLTEAFYVFVVLGLICRLFKFSLIHFIRYIFDEILLVIGTSSSES
                     VLPQLMVKLEKFGAAREVVDLVLPTGYAFNLGGLALSLPISTLFIAQVYQVPLSFGRL
                     LSLVAIMLVTSKGAAGVTGAAFIVLATTIGASGVLPIEGLALLFAVDNFLSMARALAN
                     VIGNGVATVVVAKLENDFDSAKEAATH"
BASE COUNT         2327 a         2827 c         2601 g         2168 t
ORIGIN      
        1 gtggaaccgt cgccctgcgt attgaattgt ccttcgaacg tttccagatt ccaggtttca
       61 tgaatgagcc cgacgcccag ccggacgacg gtcgtcccgt tgcccttcat atcccaagcc
      121 atacccaggc gcggctcgaa atcgaaggga tcggttttcc aaatggtgtc ccacccaggc
      181 tgcccttgct gcaccattcc tgttggcgat gacggatcga agtttccgat attgccaaaa
      241 gcgtccttca tcggggtatt caggttatag cgcagccccg cgttaacaat cagtctgggg
      301 gtaacgcgcc agtcatcctg aatgtatgca gcgtaattgt agcagatcag cttcgattgc
      361 ggcgtgcccg agaggagcgt tgccgccgat ccacccggag gaggcgaaag agttccagcg
      421 aagaaatctt caagaggagt ggaacccgcg aacgcgccgt tgccgaagaa attaaatcgg
      481 ccgcggccgt tattaaatac cgccgcatcc gcttccagat gcgtgaattc gccacccacc
      541 ttgatcgagt gcttgccctt taaaactgaa accgagtcct gcaggtccca gtatggattc
      601 ggagtaaaat attggggacg attgaacgcg gttcccacaa ctggcgtacc gccattgcca
      661 aagccgttaa tcacaatgct tggcagtcct ccggccagtg gatttgtcac tcctgtactg
      721 attccatacg tgctggaggg actattcaca tcgacattga tgaaggaaaa cgaaacgcgg
      781 ttatagccaa accgcaattc atttacgata ttggagttgg gggtatagac ccagctcgag
      841 gtaatcgtca tcgcggtaat gggcgtgctg tccgtcgcgt tttgcgagac gaaggaatga
      901 tcttctcccg tcgcagtata gtggccgtag aagaacattc cgttaaagga gttcttgtca
      961 ttggcgtgga aatcgagctt tccgacacca ttgtcgctgg tgttctgagt cggaaaagtg
     1021 ctgaggaaag tattcgaagt tcccgtattc gggaacaatt ggctgcccgc gtcaacgcca
     1081 gtacagatca caggggttgc cgttgcgttg caacccgata gcttctgact gacgttgctg
     1141 atcggaatcc ccttaccctg cagagcggtg attgcatcga ccatgctgag cgccggattt
     1201 cccacagtca cgagattcgg aaccggaatt ccgcccacga agccgatgag cgatcgcaag
     1261 ccctcgtagc ctccaaaaaa gaagagcttg tctttcttga ttgggccacc caccacgccg
     1321 ccaaactgct ttagttgggc atccgtctgc gggcactgcc cattggattg aagaagcgcc
     1381 gccgagcagc cgacaggtga cgcgatgttg aagtaattcc gtgcgtccca agcctggtaa
     1441 cggccgaatg cgtaagcgtc gccgtgaagc gtgttggtcc cagacttgat tcctacgttt
     1501 acgatcgcgc cggacttcca accgtattcc gcctttggat tttcctggag gttgaattcc
     1561 tgaatggcgt ccacgggcag gatcgtcgcg ccgtccgtga atggactcgg catgttgatg
     1621 acggggcggg cgtcaaacgg gttggagttg atgatacctt cgatgagcca tacggactca
     1681 tcaggacggg ctccgttcgt gctctgggtc catggaccac cgcccggttg cagcatcacg
     1741 ccagggcgca agcctagcag gttttggtaa tcacggccat tcaggggcaa atcgatgatg
     1801 tcggcatttt ccagcgtgcc gcccatggtt gcgtttgtgg tttccaccag tggaacagct
     1861 tccgtcaccg tcacggtttg attttgttcg ccgggctgca cgaccaggtc cactcgaact
     1921 tcgtggccaa cttcgatcag gactgcctgg cgctcaattt tcttgaaacc tttggcttcg
     1981 ccgcgaaccg tgtagttccc ggcagtcagg ttcggcgcat tgtaggcgcc cgcatcgtcc
     2041 gtcatcagcg ttctgctgat gcccctctca gtatcgatta ctgtgatggt agcgccggaa
     2101 atgacgccac cgctttgatc ggttacggtt ccaagtattc gtccaaagct tccttgtgaa
     2161 aatgcaggca ggcagtacaa aacgaccgac agcagacaaa cgagaagtgg aacatcgttt
     2221 ttactgccaa ttcgcgcaat tctcccaata cccggcttta cagaattcat gtatggacct
     2281 cctgtccaag ttgtccaatg aaccgttatt aaatctggcc ccagtcccca cgtgctgatt
     2341 ggcccaaaat aagccccggg aaaaagccaa ataattatgg ttctccggcc aacggttcca
     2401 tgaatcccga caccgctctg aattattgct ggttcggatt tctcctcagc tgcaacccgc
     2461 ttcggagtac aggtgtgaat attcccggtc ctgcgcaaag ccacagacaa gcagtctatg
     2521 agcttcgcta cccataaatg catgagtcgg atctggaaat aattgtcagc cccacccact
     2581 ctcgggttaa accgaaatcc gtattacccc ggcggacggt ccccaccgtc cttaattggc
     2641 gcgattctgt gaccgcgaga gaaatatgtc aacttttatt tcgataattc gtcgtacaat
     2701 cgcgtacagg gtttacatta taaagaagaa gttataacta ataggattat ttagttaaac
     2761 aataaacatt tcgtaaaaga accgagattg cgatcgcggc aatcgacatt agtcgctcgc
     2821 gcccaatgga agaaagtatt gcgtgccttt caccggctgt tgcaaacgcc tcaatagctg
     2881 ctgcaggtcc tcgttgcgct ccacaaaatc aatttcagac gtattgatga ccagaagatc
     2941 cgatgcggaa taatgaaaaa agaaatgttc gtacgcgcgc acgatttcct cgagataggc
     3001 gcgtgaaatc tgattttcag caggagattt tttcctggcg atgcgctcac gcaacacttc
     3061 cggagtcgcc tgaagataga tgacgaggtc cggcggtttc agcgtcgccg cgaacattcc
     3121 aaaataacgc tcatatactt ttaactcttc gttatccaga ttcacgtatg cgaaaatctt
     3181 gtctttctca aacaggaaat cagaaaccag cggcgaagca tcgttcttct gcgcttctaa
     3241 ccaccgctgg tggcgctcga tcaggaaata catctgcgcg cgaaatgcgg aacccggttt
     3301 ctccttgtaa aaatcggtca agaatggatt gtcgtcgggg tcgaagacgc gccgcgcgtg
     3361 gaggcgatcc gccaggatcc gcgaaagcgt ggacttgccc acgcggatcg gtccttcaat
     3421 ggcgatggaa cgcggcagct caaactcagg caacgcttgt gacgggaccg cgggagattg
     3481 gaccatggga cgaaattata gctgaaagac catgagtttc gcgcaccact gtgaatccaa
     3541 agatcattgc gtattaggcc agcttggcag gattacttcg tgcttgcgcg cccacgctgc
     3601 gcggcgcgaa cgggaatgca gtcggaaccg cgggaagttc gatgcgaaat gtcgcgccgc
     3661 cttcgggccg gttgtagcag gagatctgtc caccctggtc gcgaacgagt ccgtagcaaa
     3721 tgcttaagcc aagcccagtc cctttgccca caggcttcgt cgtgtaaaac ggatcgaaca
     3781 ctagattcgg atcgcgcatc cccggtccgg tatccgaaaa caggataacc acgtttccct
     3841 tttcaaacat cgttcgaacc gtgagcactc ctccgcccga ctcctgcatg gcatatacag
     3901 cgttgttgat gatgttggta aaaacctgca gcaactggtt catgtccgcg cgaacttcgg
     3961 gaatgcctgc gctcgtttgt acttcgatgc ggatgttttg cccgcggaga tcgaagcggc
     4021 ggaattgcgc ggaggtcgtg acgatcgtgt tgacgtctaa tgtcgctctt tgctcgctgg
     4081 gcgcctggcg cgcgaagctg agcaaattgt tgacgaggcc cctcgtccgc cgtgcctgtt
     4141 cacgaatttt ttcggcgatt ccgcgcgtct tctccccaat catcggatcg tccgccaaca
     4201 gttcggaata tccgagaatt gcggtaagcg gattattgat ttcatgggcg gccccgccta
     4261 ccaattgccc gatggaagcc agtttttctg actgtacgaa ctgcgtttgc agccgcttta
     4321 gattgtcgat ggactcgtgc gatgcgcgta gcaggcggag acgttcccga ttgacgaggt
     4381 catgccggag gaacgctaga aagcccagcg gcaacgccgc accgagcgtg acaagaatcc
     4441 ggaaatcacg cacggccgcc ggcgccacac tccctttcat gcaccagaat ccgatcagcg
     4501 gcagagaaag aagcgccatc cttgccagat tcgctgtcca ggcactaccg tgctgtgaag
     4561 cgtcgccggc ggctaattcc cctgactttt cggcagcggc tttagtagtc ggctcggccg
     4621 tctgcctagc gataattcca actgtcgcca accacaaaaa tgatatgagc agcggcacgt
     4681 tatacaactc gctcatcgag taagccgctc gatcgatacc gaagttgatg gttaccaatc
     4741 caaacgtgta gatggctcca acgccggcca ggtgaatata aattttgcgc cagtatccgg
     4801 tcgtcttggc tgcaagaaac gcggctccgg cggcaaatgc caagttctca agagccgtga
     4861 caatttcgtg agcatgaatg taattccgaa cattcgccgc cacgatcagc catggaatga
     4921 cggcaaatac atatacatag agccaccagc aaagcagcaa tccgaaatcc acataatcgt
     4981 aaaggatgtt gcgatcgtcc gaacgcttgt gaggctgcat ggtgagcgcg gcgatcatgg
     5041 gaacggtgtg caggaaaaaa ataatatcgc cgccaaacag attgagaacc gggcgatgtt
     5101 ggtacaccac tgcgtatgtc cacaggatct gacctgacat ccagaggacg cagccaaacg
     5161 caagcagcat ccagaacgtg cgttttcgcc agtcgggagt taccgcattc agcagcagcg
     5221 cgccatttac gaataagggg atcagacaaa atagtagatt agacgctgta gtgcggccca
     5281 cgccgatcgg tacaaattcg catatggcga tgtacacgcc taccacaagg aaccaggcgg
     5341 ccgcgacgtg ccagtaactt cggagagcta gtttcatagt ccgactaatt caccagttta
     5401 acttaaccgc gaaattggca accctctgag aggtacaggt atgacgaaaa cataacagcg
     5461 taaacatcac aaaacgaagc ggctaggcgc gctgcctcat atcgagggtc cgcgcaagcc
     5521 ccgtcgggtg ggtcgcgacg ctctcaaatc gtttgctgta cccgcaaacg gatctgcgtc
     5581 catcgtacgc atcaaaaatg tcgctggtct gttccttact tggacttcaa ggctggggcg
     5641 taaataataa atcaagaatc gaaattgtcg accgttttaa aactattttt tcgcggatgt
     5701 gtccatctgt gacgggaccg ttttttcaat ccactgtgcc ataacaccgg caatctccat
     5761 gttgtttttt tcgagcatca tcatgtgccc gttcccgtgg atgccgacat ccgcgagttt
     5821 cacccacttg acgggcacgc ccgcttgctc caaatattcc accgtgcagt gatcgtaggc
     5881 gcggtgatag gacgcttccg acgttatgac gagtattggt attttctgaa gcttgggaag
     5941 ttgccgcgcc ggagatatct gcatccagca cgagctctca ttagggtcgt caggtttctc
     6001 ccggcggaca atcgcgagat ccgatggact cgagaccggg ggatcatacg tcaacgcatc
     6061 gggtgtcggc ccccagggac gaacattctc cggaacttcg cggaaccagt cgggcgcgcc
     6121 ggtgttgtcg acgttaaaaa agggcgggcc gttcggctcg atcgccacaa tcgccttcac
     6181 cagatccggc cgcgcatctc ccactggcca gccgaacgcg ccggactgcg aatgcgtcag
     6241 cagaattgcc ggtccgattt tttcgagaag ggcaagaata ccgtcgcggt tcaagccctg
     6301 ctgcagcgcg taattggcaa tcgatggcaa ttgcgatgcg aaaaattgat cgaagatcgg
     6361 atctcccggc acgcctgccc cgggccactg cgtgtgcaga tgcgcctgcg gccaaagatt
     6421 gtagcgctcc acattggtaa atcttttctg cgttgcgacc aggtccgccg gcatatttgg
     6481 gccgtacatt tcgtcctgat aattgctgcg cccccgcccc acctggtcca caacataaac
     6541 cgcatatcca tgcctcaaaa aaaattgcgc ccagccttcc cgaccgtcag gcgttcctgt
     6601 gaaattggtg ccggactggc tgccgccgtg aaccatgatg atcggatacg gggattttcg
     6661 atgtaccgga atttggaatt ccgcataaat ctggttcacc attaccttgg catccttcac
     6721 cgtcgcgtac gatcctccgg caaatagata gccttcgcgc gcaatctcga gcggcggcaa
     6781 ttttgcattt gttgcttgct tcgcagtgga agctttcgca ggaataccgg ccggcgttcc
     6841 tgcctgcggc gcggcccaat ccgcggtccc aaatgcgagc gtcgccgtga acaaaaagac
     6901 ggcgtcacgc atcaacttac gcgcttctcg attcatcgct ctctcccccg gtgcgtcggc
     6961 ctgcctgttg cgggagagac tacactttct cttgcgcgat ggcactacta ttcaggatag
     7021 aacgtatccg aagccccagg ttcgcaaagc tcacacggaa agaataaaat ggaataatgg
     7081 cggaaagtat cgcgcaggaa aaacttcgtt tcgagaaagc ggacgccgat tttctcaccc
     7141 gcttccccga gtaccggaat acttcgctgc tggacgattt tcgcgcaacc gaataccgcc
     7201 gcctcgagga attccggcac gtttacctcg attacacagg cggttcgcta tgggcggaat
     7261 cgcaaataca gaaacatttt gatctgttgc gttccagcgt gctgggcaat ccgcactccg
     7321 cgagtcctgc ttccgtggcg atgaccgatc atgtggagcg cacccgccgc cacgtgctgc
     7381 gattttttaa cgccagcgcg gaagaataca ttctggtctt tacgcaaaac gctacggccg
     7441 cgctgaaact gatcggcgaa tcatttccgt ttgccgcagg tagccgctac ctgcttacat
     7501 tcgataatca caattctgtg aatgggatca gggaatttgc gcgcgtcaaa ggcgcgacca
     7561 ttgaatacgc gccgctcgcg atgccggatc tgcggctcga cgcgggaaaa ttggacgccg
     7621 ttctggacgg cgccgatccg cgccacgcca atttgtttgc gtatcctgcg cagtccaatt
     7681 tctccggcgt gaagcatccg ctcgatctcg tcgcgaaagc gcagagcaag ggctggcgcg
     7741 tgctgcttga cgcggctgcc ttcgtgccca cgagtcgtct ggacctgtcc gacacgcagc
     7801 cagatttcgt aacactttcg ttttacaaaa tgttcggcta tcccacgggc gttggcgcgc
     7861 tgctcattcg ccgctctgct ctcgcggcgc tgaagcgacc gtggttcgcc ggcggcaccg
     7921 tgaatttcgc gtcggtgcaa gggcaggcgc atgttctctc tccgaacgaa gctgcattcg
     7981 aagacggcac actgaattat ctttctatcc cggccgtaga aatcggattg cagtacctgg
     8041 aatcgattgg cattgacata atttcggagc gagtgcgctg cctgacggga tggctccttg
     8101 aagaactgct ggccctgcgc cacacgaatg gccgagctat ggtgcgcatc tacggccccg
     8161 caaccaccca ggctcgcgga ggaaccgtca ccctgaatct gtacgatccg cacggacact
     8221 tgctggatta ccggcgcgta gaagaactcg cgggcgcgga aaatatttcc ctccgcacag
     8281 gatgcttctg caatccgggc gcgggagaaa ttgcagaagg cttgaccgag gaagatatgc
     8341 gcgccggatt tacgatgggc gctgaaataa acctgctgag cttcgtgcgc atgatgcagg
     8401 agcgcggcca caaaagcgcc ggcgcgattc gcgcatcgat cggcgtcgcc agtaattttg
     8461 cggatgtctg gcgcttcctg cgtttcgtcg ctggcttccg cgatcaaacg cgcctcacaa
     8521 tcggcgaggt cagcttcgac atcgaatcct gccgcatcat cagagatgga agctagcttc
     8581 acgtggcata ggcttcagcc tgtggaattt agtccttgca aggccaaaac ccacacgctg
     8641 aagcctatgc cacataaccc gattaatgcg tcgccgcttc tttcgccgaa tcgaaatcat
     8701 tctccagctt agccaccacc acagtcgcca ccccgttccc aatcacattc gccagcgcgc
     8761 gcgccatgga cagaaaatta tccaccgcaa acagcagcgc gagcccttca atcggcaaaa
     8821 ctccggacgc accaatcgtc gtggccagaa caataaacgc agctcccgtc accccggcgg
     8881 ctcccttgga agtgaccagc atgatagcca caaggctgag cagccgccca aacgacaacg
     8941 gaacctgata aacctgtgca ataaacagcg tactgatcgg cagagacaat gccagtccgc
     9001 caaggttgaa cgcgtacccg gtaggcaaaa ccaggtcgac cacctcgcgc gcggccccaa
     9061 atttttcaag cttcaccatc agctgcggca aaacgctttc cgaagaagac gtcccaatca
     9121 ccaacagaat ttcatcaaaa atgtagcgaa taaaatgaat gaggctgaat ttaaatagcc
     9181 ggcaaatcaa tcccagaacc acaaacacat aaaacgcctc ggtcagatac gtgcaaacga
     9241 tcagcttgga caatgcgaac aacgcaccgt tgccaaattt cccgaccgta aatgccatag
     9301 caccgaatgc gccgatgggc gcaacgcgca tgatgatgcg aataattcca aagaacgcca
     9361 tcatgattcc gtccaggctg tgcaccacgg ctttcccgcg ctcccccatg ccaatcatag
     9421 ccatgccgaa cagcagcgcg atcaccacca cttgcagcaa atcgccgcgc acaaaggagc
     9481 caagaaaatt atccggcacc gttcccagca aaaaaccaag caccgtaaaa ttttttccgc
     9541 tgcccgcgta cgctgaatag tcggtggaag tcgcaccggt ttgttgcgcg ttaacgccaa
     9601 cgcctggctt aaaaataacg accgaggcaa gcccgatggc cagcgccaga gtcgtcacca
     9661 gttcaaaata aatgagcgcc ttgaggccta tgcggcccac tttgcgcacg tctccgatgt
     9721 gcgccactcc cgtcgaaacg cacagaaaca caatgggcgc gatcgccatg cggatgagct
     9781 tgatgaacag atcgcccagc ggccgcatct gcacggcgag tttcggatcc acctcgccca
     9841 aaataatcgc ggccacgatg gcgataaata catggagcgt aatattattt ttccacgtgc
     9901 gcttcttctt cgggtgcatc tcg
//
LOCUS       DASZMB010000079         5090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1462296, whole genome shotgun sequence.
ACCESSION   DASZMB010000079 DASZMB010000000
VERSION     DASZMB010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1462296"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VG272_03745"
     CDS             <1..900
                     /locus_tag="VG272_03745"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWF12824.1"
                     /translation="LGKTGAQVSAIGIGGYHLGSTRDQAEANELVSRAMDAGINFFDN
                     AWDYHDGMSEERMGIALKGKRDHAFIMTKVCTHGRGKDVALRMLEESLRRLQTDHLDL
                     WQIHEVIYYNEPDLYFSAGGAIEALEAAKQQGKVRFVGFTGHKDPAIHLRMLSHGFPF
                     DTVQMPLNCFDSTFRSFETQVLPEAIRQGVAPLGMKSLGGGGEFVKHGAITAQEGLRY
                     AMSLPVATTISGMDSLEVLEQNLGVARGFQPMSKEEMASLRERVRSVASDGRYELFKT
                     SKHYDGDYGREQHGFPKSDELPA"
     gene            1333..2862
                     /locus_tag="VG272_03750"
     CDS             1333..2862
                     /locus_tag="VG272_03750"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWF12825.1"
                     /translation="MSEAATSHEEVMKKTHTKLKFNALNFWEILAQAVALISPSMTAA
                     LIVPLMFGTAGTAGWLCYLFGTIMLLFVAFSLNQFSRRYTSAGSMYEYTVKGLGPKAG
                     GISGWCLIWAYMFIGIAGVTGFTHFATKLLGLTGASGYLSHPSITLFAICALAAWFLA
                     YKDITFSTILMLVFEGISVLLIVMLAFLALGKTGVADPAQFHPVGHGLKDIGLGVVVA
                     IFSLVGFECATAFGEEAREPLKTIPRAVIASLLLTGTFFVFITYVETHALAGSTPPLD
                     QLDAPLSTLSANLGVKWMGVLISMGAMISFFALALSCLNAGARILFTMGRYGLFPVSI
                     GSSHKENLTPHIAISLFAAIQFLIPTSFILLSYAAVGGWQMAPFDAFNDAGLFGAMGF
                     CGAYVLISLATPFYLKKIGELKPHHVAVAVISLLLLLVPIVGTVYPVPAAPLNYFPYI
                     FLAYIIAGIVMVSMRSRSHSEMDGIRKVLEETTMGQTVSTPSAIAPIAEGRLNPTSGA
                     A"
     gene            2859..3671
                     /locus_tag="VG272_03755"
     CDS             2859..3671
                     /locus_tag="VG272_03755"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HWF12826.1"
                     /translation="MMNNLGVLASLHRYPVKSMMGEALNAAEVTGSGLCGDRSYALRD
                     TETGKIVSAKNPRKWPNLFSYHATYTDSPKSGADMPPVRVTLPDGGAAVSTMADFSAT
                     LSAKLGRPVTLLAAPPHDPQLEEYWPDMEELSNRDVVTDEAMPQGTFFDCATLHVLTT
                     GTLDRLRELSPRSRFEPRRFRPNIIADTGDQKGFVENDWIGKVIAIGPEVLIKINGPC
                     PRCVMTTLSQADLPQDTGVLKTAANYNAAKVGVYASVIQTGTIRIGDTLSVV"
     gene            3668..4372
                     /locus_tag="VG272_03760"
     CDS             3668..4372
                     /locus_tag="VG272_03760"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HWF12827.1"
                     /translation="MNSIFSIPKAVLESVQIGAIRNLGHQNAADIHDKPWTTGFFKIP
                     VRGPVHVSMTNVDGDGQADLVNHGGADKAVLAYSADHYPKWRKELQLPEMPYGAFGEN
                     FTISGLNEELVCIGDSFRIGAVKVRVSQPRQPCWKLARRWRMHELTGFVVRNGRTGWY
                     FRVEEEGEVEAGMPVTLIDRPNPEWTVARANQILHHMKLDLAATLELSCVPELAASWV
                     EELQERADRLRALSPQ"
     gene            complement(4563..>5090)
                     /locus_tag="VG272_03765"
     CDS             complement(4563..>5090)
                     /locus_tag="VG272_03765"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF12828.1"
                     /translation="NIQHATAADDDDIRAIVQKHLPITSTFLDHRQDEPGDLQKWAPY
                     SRYRLMEVTWKKMLAAGVVLGWGSGSAPPPGRVYNTACNCSHGVQGEQFPLLVSWGAS
                     PVYTLRMATTVNAGIIRMSDKLGTIEKGKFADIIAVSGDPLQDISQMQHVKFVMKGGD
                     VIKNDVAMAGAMSGN"
BASE COUNT         1146 a         1377 c         1409 g         1158 t
ORIGIN      
        1 ctcggcaaaa cgggagcgca agtttccgcg attggcattg gcggttacca cctgggctcg
       61 acccgcgatc aggcggaagc caacgaactc gtgagcaggg ccatggatgc ggggatcaat
      121 ttttttgata acgcgtggga ttatcacgac ggaatgagtg aagaacgaat gggcatagcg
      181 ctcaagggca agcgcgacca cgcgtttatc atgacgaaag tttgcacgca cggccgcggc
      241 aaggacgtag ccctgcgtat gctggaggaa tccctgcgtc gcttgcaaac ggaccatctg
      301 gatctctggc agatccacga agtgatttat tacaatgagc cggatttgta tttttccgcc
      361 ggcggcgcga tcgaagcgct ggaagccgca aaacaacagg gcaaggtgcg cttcgtggga
      421 tttacagggc acaaagaccc cgcgattcac ttgcgcatgc tctcgcacgg atttcctttc
      481 gatacggtgc aaatgccgct gaactgtttc gatagcacat ttcgtagttt tgaaacgcaa
      541 gtgctgcccg aagccattcg ccagggcgtc gcgcccctgg gcatgaaaag tctgggcggc
      601 ggcggagaat tcgtaaagca cggcgcaatc acggcacagg aagggttgcg ttacgcgatg
      661 agtttgccgg tcgccacgac catcagcgga atggattcgc tggaagtgct ggagcaaaat
      721 ctgggcgttg cgcgcggatt tcaacccatg agcaaagaag aaatggcctc attgcgcgaa
      781 cgcgtccgct ccgtcgcgtc cgacggacgc tacgaacttt tcaaaacttc aaaacattac
      841 gacggcgatt atggccgcga gcagcacggt tttccgaaga gcgacgaact gccggcataa
      901 catttttccc gggagaaaca aggtgcgtgc ggaagaaatg cgcgtgtgat cgcgcaactc
      961 tccgaacacc agcggacacc ctgccttgga gttatccaat cgatatcgaa gttattgaaa
     1021 tattcgattg gacaaagatc ggggccgtgc gtactttgag gacgagaagt ttcggatcgc
     1081 tttgcagaag gcgattcgag cctatgaggt ctggccgcga atgccctgaa taggcctgta
     1141 acatattttt tcgctcgcca agaatgctga gccgtcgccc tgtcggtagt tgttttgcaa
     1201 gtttgacgct gatgaattga gtcttacccc caccgtgaag ttgggctgtt tccaaaataa
     1261 cgacaagcga tgcttgcgtg acggcgaggt ctgacacgcg ctcattcacg ccgctatcca
     1321 gggggagaca ccatgagcga agcagcaacc agtcacgaag aggtaatgaa aaagacccac
     1381 acaaaactaa aattcaatgc gctgaacttc tgggaaattt tggctcaagc ggtcgcgctg
     1441 atttctccca gcatgaccgc cgcgctgatc gttcccttaa tgtttggcac ggctgggacg
     1501 gcaggctggc tttgttatct gtttggcacg atcatgttgc tctttgttgc cttcagcctt
     1561 aaccaattca gccgccgcta cacttcagcg ggcagcatgt atgaatacac ggttaaggga
     1621 cttggtccga aagcaggtgg catttctggt tggtgcctga tctgggccta catgtttatc
     1681 gggatcgccg gcgtcaccgg cttcacgcac ttcgcaacca aactcctcgg gctaacaggt
     1741 gcgagcggat atctatcgca tccatcgatc acgctttttg cgatttgcgc tctggctgcg
     1801 tggttcctgg catataaaga tattactttt tccacaattc tgatgcttgt atttgaaggc
     1861 atctccgttc tgctcattgt gatgctcgca ttccttgcgc tcggcaaaac gggtgtcgcg
     1921 gatcccgcac aatttcatcc ggttggccac ggattgaaag acatcggcct gggcgtagtg
     1981 gtggcgatct tcagcctcgt gggatttgaa tgtgccaccg cgttcggtga agaagcgcgc
     2041 gagccattga aaaccattcc tcgcgcggtg atcgctagcc tgctgctcac cggcactttt
     2101 ttcgttttca tcacgtacgt tgagacgcac gcgctcgcag gaagcactcc gccactcgat
     2161 caactcgatg cgccgctctc cacgctcagc gcaaatctcg gcgtgaagtg gatgggcgtt
     2221 ttaatttcga tgggcgcgat gatcagtttc ttcgccctcg cgctgtcctg cctcaatgcc
     2281 ggcgcgcgca ttttatttac aatgggacgt tacggattat ttcccgtttc catcggcagc
     2341 tcgcataaag aaaatctcac cccgcacatc gccatctctt tattcgccgc gattcagttt
     2401 ctgattccca cgagtttcat cctgctttcg tatgcagccg taggcggatg gcagatggcg
     2461 ccgttcgacg ctttcaatga tgccggatta ttcggcgcca tgggattctg cggcgcgtac
     2521 gttttaattt cactggcgac gccgttttac ctgaagaaga ttggcgaatt gaaaccgcac
     2581 cacgtcgccg ttgccgttat ttcactattg ttattgctgg tgccgatcgt aggcacggtg
     2641 tatccggttc ccgcagcgcc cctgaattat ttcccttaca ttttcctggc gtatatcatc
     2701 gccggtatcg tgatggtttc aatgcgtagc cgctcgcact cagaaatgga cggcatacgc
     2761 aaagtactgg aagaaactac catggggcaa acagtttcaa ctccctccgc gattgcaccg
     2821 atcgcggaag ggcgactgaa ccctacgagc ggagcagcat gatgaataac ttgggcgtat
     2881 tggccagtct ccaccgctat cccgtgaagt ccatgatggg ggaggctctg aacgcagcgg
     2941 aggtcactgg aagcgggctg tgtggtgaca ggtcttacgc gttgcgcgat acggaaaccg
     3001 gaaagatagt gagcgcaaaa aatccgcgca aatggccgaa tcttttcagc tatcacgcta
     3061 cgtacacgga ttcgcccaag tctggagccg atatgcctcc ggttcgcgta actcttcctg
     3121 acggaggtgc agccgtcagc acgatggccg atttttcggc cacgctttcc gccaagctgg
     3181 gccggcccgt caccctgctg gcggcgcctc cgcacgaccc gcaattggaa gagtattggc
     3241 cggacatgga agaactttcg aaccgcgatg ttgtaaccga cgaagcaatg cctcagggaa
     3301 ccttcttcga ttgtgccacg ctccacgtgc tcaccacagg tactctcgat cggctgcgcg
     3361 aactcagccc gcgcagccgt ttcgagccgc gccgttttcg ccccaacatc attgctgaca
     3421 ccggtgatca gaaaggattc gtggagaatg actggatagg gaaagtgatc gccatcggac
     3481 ccgaagtgct gatcaaaata aatggaccgt gcccgcgctg cgtgatgacc acgctgagcc
     3541 aggccgattt gccgcaagat acgggggttt tgaagacagc tgccaattac aatgcggcaa
     3601 aggtaggagt ctacgcgtca gtgatccaga ccggaaccat tcgaatcggt gatacattat
     3661 ccgtagtatg aatagcatct tttccattcc taaagcggtt ctcgaatccg ttcaaatcgg
     3721 ggcgatccgc aatcttggtc atcagaacgc cgccgacatc cacgacaaac cctggaccac
     3781 tggatttttc aaaatacctg tccgcggtcc cgtgcacgtc agcatgacga atgtggacgg
     3841 agacggccag gcggacctgg taaatcacgg cggtgcggac aaagccgtac tcgcctacag
     3901 cgctgatcat tatccaaaat ggcggaaaga attgcagttg cccgaaatgc cgtacggcgc
     3961 tttcggcgaa aattttacga tctccggcct gaacgaagaa ttagtttgta tcggcgattc
     4021 gttccggatc ggtgccgtga aggttcgggt gagccagccg cggcaaccct gctggaaact
     4081 tgcgcggcgc tggcgcatgc acgaactgac aggattcgtt gtgcgcaatg gacgcacggg
     4141 ctggtatttt cgcgtagagg aagaagggga agtcgaagcc gggatgcctg tcactctgat
     4201 cgaccgtccc aatccagagt ggaccgtcgc gcgcgcgaac cagattctcc accacatgaa
     4261 actggatctg gccgcgacct tagaattatc ttgcgtgccg gagttggcgg catcttgggt
     4321 agaagaattg caagaacgcg ccgatagatt gcgcgcgctc agccctcaat agtacacaag
     4381 cctcaggatt caatttaagc gtcatccctg aagattttaa aatcgaagtt aagatcacaa
     4441 aaggaaaatt ggttcgaggc gagaaaaatc acggaggact taactccggc aaaccgtaac
     4501 ggcagaatgg cctttaaaaa tcaaagggtc cctgccgtta cgatcgccag atatttctaa
     4561 tgctaattac cggacattgc gccggccatc gccacatcat tcttgatcac gtcgccgccc
     4621 ttcatcacga atttcacgtg ctgcatttgt gaaatgtcct gcaaaggatc gccggaaacg
     4681 gcgatgatgt cagcgaactt gcccttttcg atcgtgccga gcttatccga catgcggata
     4741 attccggcat tcaccgtggt cgccatgcgc agcgtgtaca ccgggcttgc accccagctt
     4801 acgagcagcg ggaattgctc accttgcacg ccgtgcgagc agttgcaagc ggtgttgtaa
     4861 acgcgtcctg gcggcggtgc ggaaccgctg ccccagccga gcactacgcc cgctgccagc
     4921 attttcttcc aggtaacctc catcagacga tagcggctgt acggagccca cttttgcaga
     4981 tcgcccggtt cgtcctggcg gtggtcgagg aaagttgaag tgatgggcag gtgcttctga
     5041 acgatggcgc gaatgtcatc gtcatctgcc gctgttgcgt gttgaatgtt
//
LOCUS       DASZMB010000080        30137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2835395, whole genome shotgun sequence.
ACCESSION   DASZMB010000080 DASZMB010000000
VERSION     DASZMB010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 30137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30137
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2835395"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VG272_03770"
     CDS             complement(<1..187)
                     /locus_tag="VG272_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12829.1"
                     /translation="MTAQARKSKSIWDGPIARRAVGDSFVKLNPATLMKNPVMFVVEV
                     GSALLTVELISHMIRHTQ"
     gene            complement(198..1964)
                     /gene="kdpA"
                     /locus_tag="VG272_03775"
     CDS             complement(198..1964)
                     /gene="kdpA"
                     /locus_tag="VG272_03775"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HWF12830.1"
                     /translation="MTNNGWFQIIFFLLVVLALTKPLGMFMTRAFNREKTFLDPLMRP
                     VEKLVYGLTGVDETKEMGWTEYAGAMLTFSGVTMVVLYLIQRLQNHLPLNPQKLAAVD
                     PALAFNTAASFTTNTNWQAYVPETTMSYLTQMAGLAYHNFISAAVGIALAIAIIRGIV
                     RREQKTLGNFWVDMTRATFWVLLPSCVLGALLLVWQGVPQNLKPYATAELMDPQIVQT
                     TDASGKTTTQIVKEQTIAQGPVASQEMIKELGTNGGGFFNANSAHPYENPTPLTNFFE
                     LVLIFAIGSALTYTLGQMTGSPRHGWAAWCAMAILFLVGVTAAYSAEARGNPLLQSTA
                     TQTASTGNMEGKEVRFGIANSALWAVVTTDTSCGAVNSMHDSFTPLGGMIPLINILLG
                     EVVFGGVGSGIYGIVVFIILSVFIAGLMVGRTPEYLGKKIEAFDVKMAMLTVLIYPLM
                     VLTFTAISLVAPKFGLTSLANAGPHGLSEIFYAYAEAGANNGSAFAGLNANTLWYNTT
                     IATTMLFGRFFMVIPVLAIAGSLVQKKHVPETSGTFPVTSPLFTGLLISVIVIVGALT
                     FFPALSLGPILEQLLMHAGKTF"
     gene            2865..3755
                     /locus_tag="VG272_03780"
     CDS             2865..3755
                     /locus_tag="VG272_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HWF12831.1"
                     /translation="MLTVLLALAFLCVSNARAQERADCRVIHSVILHSPVRFCVYLPA
                     SYSEPGAKTRKYPVLYLLHGLGGTQQSMAVNGEWTELQDLRRENKVGDFIVVSPEAWD
                     TFYINSRDGQTPYSDFFLREFMPYVERIYRVQGTRITRGITGFSMGGYGALRFAFLHP
                     ELFGSVSAHSAAIERDPPNPVEGIPSPGNFASEILGKTFGDPINKQFWFQNSPYVLAR
                     KNAAELRRMKIYFDCGTEDTYGLEAGATEMHEALSALKIPHEFHLYPGGHTGSYLVEH
                     RSASFEFHWHVFHSALDAAK"
     gene            4135..7797
                     /locus_tag="VG272_03785"
     CDS             4135..7797
                     /locus_tag="VG272_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HWF12832.1"
                     /translation="MAVPLKLFAGVSVAVSPASVNLSPNGAQQFTATVTGASNTSVTW
                     SIQQGATGGTILGSGLYTAPGAVGNYVVIVTSNADNTQSAFATVSVAGFIHAGLLNPD
                     PVTATLLASGMVLYTDGGPSGFVTSPAEIYNPATSTSNATGSMTILRWLETATLLGNG
                     KVLFAGGQTSSGTTATAELYDPVTGMFAATGSMTVPRQGHTATLLPDGKVLIVGGVVG
                     NCNSSCSYNTAEIYDPAAGTFSLTAGNLPSPPGSVASILLLTGKVLVAGGGSGGSAEL
                     YDPGTGFFTATGTLVNQNDAFSATLLQSGKVLFVTGGNNGVAASTAEIYDPSSGTFTA
                     TGNLQLARGFDTATLLKSGQVLIVGGLSSAPAELFDPATGTFSLTGSVQEMRISQQAT
                     ALPDGTVLIAGGSSVFQGLSSIEVYDPSAKSFSGQSVFMKAARSGHAAAQLADGRLLL
                     TGGEDAFLNVISSAEIYDPVSGGFTLTGPLIQGRHGHTATLLGNGNVLIVGGYEDSAG
                     AQNGNTLVATAEIYNPASGTFSLISGPNLARAYHTATLLPNGKVLIAGGVTAAQLQSP
                     TSSVEIYDPVMQAFTQAGNMFAVRYNHAATLMNDGRVLISDGLTIAGTPGNGIGLDEI
                     YDPSSGQFAQAGPKEVINNHSAAPTASILLQNGQVLADNQSIFDPVSNSLTTLSSLVT
                     LQTLLQDYEFALLPGGQVLTTSNGYATYLFDPTSQTYSQSGSMQYFRSRPTLYTLPNH
                     EVMVAGGVGIAQVEIYVPPTASANAAPALSAINPSTVVAGGAGFTMLVEGSNFVSNSV
                     INFNGAARQTTFLSTTQLSTALLPSDIANTGTATITVSNPASGSATGITSNPLTLSIL
                     AANIQPVIGALSPASTTAGGAAFILLLSGSGFTQNSAVTFNGNHVPAAFISVTQLQAN
                     IPAGAIVTAGSFLVAVGNPGGNPTTVVSFTVNNPVPQENLLSPASATAGSAGVTLDVN
                     GSNFNLSSKVLINGAVHTTTYVSSTLLQTGLSASDLLKGATLNISVSNPTPGGGTTSM
                     LPFAVTDYSVAVPVPTVTVSAGQTAVFNLTVAPSNGTFANSILFTATGLPSGAVASFS
                     PSATVTPGATSQTVILSITTIAHAFSTTAYFPRLARPTVMCLSVIGIAFALVGLVFIS
                     SKTAALQWKRFAPRLITAILLIGVASTMACSTGGGASAPQANPASGTPAGSYTITITG
                     ATGGIVHSASATITVI"
     gene            8130..10970
                     /locus_tag="VG272_03790"
     CDS             8130..10970
                     /locus_tag="VG272_03790"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HWF12833.1"
                     /translation="MRTIFAVLVSFFICAVFCQPLMGQAPPPPFLFLLEGKGVPTGTV
                     HAFRMNSATGGIAEVPGSPFNAGLVPNQLIVDPTGRFVFVTNQESEDVTAFAIDHSTG
                     ALAQLPGSPFLIGAQPVTAAVDPTGRFFYVFATSNVYGADQELLYEYTIDAITGVLTL
                     SSPSPTTWEFGQGILISNITFDSGGNYAYLGQIQSGMFGAPTLICAVDFGSGNLSITG
                     SVQPANNGEANYIAVSPTGSFLYSGNAAGNVANAFAISAIGGQLTEISGSPYLMLNAP
                     SSLVVHPSGNFLYIANENQIYQTTEQPSQYNGSVSAFSINAVDGRLMQVSGSPFAAGI
                     NPVSVITDPTGRFVYSASSMYTGGFTSFAQIMGFSVNVSTGALTPFSSPAFTDSVNGN
                     GAQLAISNGLLPTNPVPMISSLNPPSSIATDTAFTLQVNGANFVPGAAVYFGGQFRST
                     TFVSSTQLNVSILASDVDNNGNIVVFVFNPLPGGGASTSVEFPVSALVPTISTTNPTS
                     VIAIPNVFDFFCIGTNYTASSVINFNGMPLFTLYVGPTLVLGSAPAGDVAAPGIVTIE
                     VVTPSNGVPGGGTSNPVTISILPPFVQLSVSDISPTSATAGGPGFVLTVTGSGFVPSS
                     QVSFNLLNVPTTVITSTQLIATIPASAIAAAGNPYVIVTNPGGATSAASTFTVNNPLP
                     GTGAIIPQSLPAGSNALTLNVTGTNFTPNSVVLVNGVARSTTYMTSTLLLATLLPADL
                     MHSATLNITVSNPPPGGGTTAALALSVADYNVSAVSTAQTLTAGSQANYNLMISAVNG
                     TLGHAIVFTASGLPPGTSGTFSPASVPAGGGSSNVMFSVTTASRSSSAYLRFDSSARS
                     KWPFFAGILFSLAAMWIWFPLRNGRMRLATPLFLVSLLLCVLLSLTACGSMLISPPAQ
                     INTGTPSGTYMINLAATSSNITINTQITLTIM"
     gene            11194..11907
                     /locus_tag="VG272_03795"
     CDS             11194..11907
                     /locus_tag="VG272_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12834.1"
                     /translation="MNAAGQCVQPPPMVQLRDYNGPFHKVIGTFTQKLDRQSVPDPHY
                     KQGALLCSLKVKDKLFLYLHDTVDPETFISAGFNAGISQAENDDPSFGQGGKGYAQRL
                     GASYVDQVQVRFFKEFAYPIIFSEDPRYYRMGQGPPKKRFLHAVAHAVVAYNDKGRPM
                     FNFSEWLGEISGASISNLYHPGNERGVLPAAQNIAIDVALDIGFDELKEFWPETARKL
                     RLPFRDQNVPQPVADPAAR"
     gene            complement(12040..13059)
                     /locus_tag="VG272_03800"
     CDS             complement(12040..13059)
                     /locus_tag="VG272_03800"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HWF12835.1"
                     /translation="MIHKTISRRKFTHGLLAAGGMLLMTGRARAADFELRQFHNQPAD
                     SPLDQWLSKMWAAVKTETNGRVQVQVLPDNNKIAGGDPAALNMLVSGEVDFFTLNGGS
                     IGNIVPASNVQGLLFAFRSTAQIFESLDGELGDYLRQEMKAKGIYGLPKGCFDNGMHQ
                     ITCATRPIRTVDDLQGLKIRTPDAPIYTEAWKAMGASPVVANFNKLYETLKAGTAEAQ
                     TNPLAVAEFLKLYEVQKYVSMTNHGWSGFNLMANLKLWERFPADVQAIIERNVVKFVR
                     QQREELTALNTEFRTKLATQGMTFNDADTSSFRPRLSSYYAHWKEVVGERAWTLLEAR
                     TGKLA"
     gene            complement(13194..14879)
                     /locus_tag="VG272_03805"
     CDS             complement(13194..14879)
                     /locus_tag="VG272_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12836.1"
                     /translation="MSKIRKPSLSAKENTGEVNGTRKAFTRRSLLKSAVSAAVGATAA
                     GLAGKLGAGAQVQGLYTTNTAPEIPLPLGSLTYIDKKQYIHNMEIISHISGSSISGGE
                     PLMVMWAKGKQRLLPAGGDFVDISEAKNPVLLNKKRVIQGNGPVVYNTKLKKWIMMCT
                     AAQPLTSATPQYPHGQYDKELRDKVLAYKGLRGIRNYDVTDPTKPNLLQEYSTGVKGN
                     GTHHNFYDGGKYAYLDCGWDETLRLENHQRPYSNALMIVDLSDPANVQEVSRWWVPGQ
                     KLGEEEEYKKYRFAGDKTSWTGNHGALTVPKRVEDGGTIGYGGFGAFGFYAMDLRDIK
                     NPKPYGHMQYEFDTFGTIPFHTCYPVIADAAHPQLMNKIVAVHEALEADCRENYHTPY
                     MVDVKDPKNPKIIGFFPRPAAPSDAPYTDFCFARGRFGSHNTQCWLAPGTSNPSIVVI
                     AWFNAGVRVFDISNPTQPKEIAWFVPPLEGEINDYASWWRGTTENVFVEFDRNLIWIG
                     THEGSYCLSCPALGKANTEPTKVAKWSISHCNVGWDDQTARSFYFGRSTSQAG"
     gene            complement(15082..16428)
                     /locus_tag="VG272_03810"
     CDS             complement(15082..16428)
                     /locus_tag="VG272_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12837.1"
                     /translation="MKKIGLMFLILVIGMTIGVRISGTLSPVEGQSKPGTGFAAVPGA
                     VGGEDLSGGYEVVKNWPKDISTLPGNEKWTYGAGESVFAESPNRIYALYRGELPKMVP
                     PKPMLLPEAGPSISYPVAGFWRDATVASLPGTGGTDQDTREWLTSWEGKSEKLGIKGP
                     PYRELGKDAKWENCLVVYDGQGNIIETWKQWDKLFRRPHSVYISPYDPEKNVWIVDDN
                     MDTIYRFTHDGSKLLQTIGTPEIPGEDANHFNRPTYIDWLPDGTFYVSDGYTGTRIAK
                     FDKEGKFLMQWGIKGGPRRGGDNKPLETRPGYFSNVHGVAIDQKTRHVFVNDRNNHRI
                     QVFDENGKYLYEWKIDNAPSSLHLLYIGTGSTIWTFDRTTNKMIEYDLQGHLLYSWGS
                     MGDYPGYLWGVHGISVDQEGNLYVAEVDAGRIQKFTPKPGANPAFLLAKPVYSAWK"
     gene            16790..18892
                     /gene="tkt"
                     /locus_tag="VG272_03815"
     CDS             16790..18892
                     /gene="tkt"
                     /locus_tag="VG272_03815"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HWF12838.1"
                     /translation="MSVTDKTASMQNTSTSPIDMLCINTIRTLSMDAVQAANSGHPGT
                     PMALAPLAYCLWQEFLRYDPNDPVWPNRDRFVLSAGHASMLLYSVLHLAGVKDVGENL
                     EILTGPSIRLEDIKNFRQLNSRCPGHPESHLTTGVETTTGPLGQGYANSVGMALAGKW
                     LAARYNQPGFDEVYNYNVYALGSDGCMMEGISSEAASLAGHLRLSNLCWIYDCNRITI
                     EGGTPLAFDEDVAVRFAGYGWNVTHVTDANDLDMIRTGFRTFLATKDRPTLIIVNSHI
                     GYGSPNKHDSNAAHGEALGEEEVKLTKRFYGWPEDAKFLIPDGVYEHFQKGIGTRGKA
                     ERDRWTAKQIEYRVAHADLANEIKLMQYRELPSGWDKDVPVFPADAKGLATRDSSNKV
                     ENAIAKNVPWLIGGSGDLAPSTKTLLTFEDTKGFQAGNYGLRNLHFGIREHAMGGVIN
                     GIAQTKVRAFGATFFVFTDYMRGAMRLSAIMGMPVIYVLTHDSLGVGEDGPTHQPIEH
                     LAIARATPGMLTLRPADANEVAEAWKVVMQLQHKPALLVLTRQTVPTLDRTKYAPASG
                     LARGAYVLAEKPGITPDVLLLATGSEVSLCIEAFEQLSKEGIQARVVSLPSWELFEKQ
                     DQAYRDSVLPPAVKARVSVEMATTFGWERYVGTSGYMIGMHSFGASAPIKALQKHFGF
                     TVENVVAAAKEQVQSSKK"
     gene            18990..20564
                     /locus_tag="VG272_03820"
     CDS             18990..20564
                     /locus_tag="VG272_03820"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF010696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HWF12839.1"
                     /translation="MDASELWKRYRQYHCHVDSLDLSLDISRMHFDDAFLASMEPAMQ
                     KAFQEMDALESGAIANPDEKRMVGHYWLRTPQLAPTKEIALEIAGTVESIQKFAADVH
                     AGKIRPRNAERFTQVLAIGIGGSALGPMFVGDALGGPASDRMTIHFIDNTDPDGIERM
                     IDVLKVLLPETLVIVTSKSGGTPDTRNGMLVVAEAYRAAHLNFAEHAVAVTMAGSHLD
                     KLAASEAWLARFPMFDWVGGRTSELSAVGLLPAALQGIDIVRMLAGAAACDVATRIHE
                     TRRNPAALMALMWYVVTDGKGKKDMVVLPYKDRLLLFSRYLQQLVMESLGKQLDLDGK
                     RVEQGIAVYGNKGSTDQHAFVQQLRDGVNNFFVTFVRVLESGGSPLEVEPGVTTGDYL
                     DGFYIGTREALYGNDRQSMTITVHRVDARTIGALIALFERAVGLYASLIHINAYHQPG
                     VEAGKKAAAAVLSLQAKTHAALSGMPQTATQIAAAIGAAESAESVYLILEHLSANGRA
                     RLAPAANPGNATFTRA"
     gene            complement(20770..22227)
                     /locus_tag="VG272_03825"
     CDS             complement(20770..22227)
                     /locus_tag="VG272_03825"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HWF12840.1"
                     /translation="MSRPIQVMKFGGTSVGSADRIHSAATIAAHASKDRAVVVVVSAM
                     SGVTNMLIAAAGKSATGDEAAAEALTRSLQEKHHETIGKLISDPENRRQLLSEIDALI
                     ERAANYCRGCALLGELSPRALDVIAGTGERLSARMTAAVLREMGLRGVAFDATELIVT
                     DDVHGGARPLSEPTRTKTQSMLLPLLEANGIPVVTGYVAATAKGIPTTLGRGGSDYSA
                     TILGAALDASEVIIWTDVNGVLTADPRMVPNAATLDEVSYTEAGELAFFGAKVLHPMT
                     LRPMIESAIPVWIRDSFHPDLVGTKITQSVKPAPQGVKAVTATRDVAMIAIAGPGIIG
                     VPDIAARIFSATASAHANIVMISQSSSQDSICFVVQAADATRTEEALRAALHDDVHQH
                     DLDHIKVIRNVAIVAAVGENMAGTPGIAGRVFSTLGKESINIIAIAQGSSEYNISFLV
                     ESSGMEKAVKALHQAFGLGKQEKSDDARTTAQVTS"
     gene            complement(22224..23333)
                     /gene="asd"
                     /locus_tag="VG272_03830"
     CDS             complement(22224..23333)
                     /gene="asd"
                     /locus_tag="VG272_03830"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF006416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HWF12841.1"
                     /translation="MSNKKVRVGILGATGMVGQRFVQMLEHHPNFEITALAASDRSQG
                     KTYGEACTWRLVGDMPAFARSMVVQPPQPPLDCDVVVSSLPSDVARSSEEAFARAGYP
                     VISNASSFRMDEDVPLLIPEINSDHLKVLERQKRERGFSTGYIVTNPNCSTIIMALAL
                     APLHNKFGIEAVIATTLQAISGAGYPGVPSLDVVDNVLPFIDGEEPKMESETRKILGT
                     IGKTSSFENAAFPVSAQCHRVNVADGHMVAVRVKLAKRASIEEVREALASYTSVPQEL
                     KLHTAPKHPILVRGEKDRPQPRMDRDAGGGMSVTVGRLFPDSVFDYRFVVLGHNTVRG
                     AAGAAILNAELLCARGLLHAKSANENAAPAEVRVR"
     gene            complement(23323..24141)
                     /gene="cobA"
                     /locus_tag="VG272_03835"
     CDS             complement(23323..24141)
                     /gene="cobA"
                     /locus_tag="VG272_03835"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HWF12842.1"
                     /translation="MAGKVYLVGAGPGDPELLTRKAWRVLQSADVVLHDDLVPQDILC
                     VLPVTTQVVNVGKRCGAKSISQDEINALMAQLAREGKIVARLKSGDPMLFGRAGEEME
                     ALRAAGTDFEIIPGVTSALGGAASAQISLTDRRRASRVVFVTAHREGGTGLRNEGHIA
                     TDTTYVIFMPGHRYAELSAELQAAGLGAEIPCALISRATSKDETIFRTTIGKLQECPA
                     AASPSLLVVGIVAGIDADETAARDVTFSGKASEEILQLAQQHPYHDLHGRGSVE"
     gene            complement(24289..24861)
                     /locus_tag="VG272_03840"
     CDS             complement(24289..24861)
                     /locus_tag="VG272_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HWF12843.1"
                     /translation="MSLFPMFLKLEGRLILMVGAGTVGEGKIESLLASGATVRVIAPQ
                     ATPRVREWAREGRIQWEAREYKPSDFAGVFIAIAATGSTTLHDEIYAEAKSRGILFNA
                     VDEPERCDFYFPAVVRRGDLQIAISTGGLSPALAQRLRKQLEEQFGPEWEEWIAKLGR
                     TRDELKSIPMPPEQRKRLLHQYASLDPKPH"
     gene            complement(24979..25677)
                     /locus_tag="VG272_03845"
     CDS             complement(24979..25677)
                     /locus_tag="VG272_03845"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HWF12844.1"
                     /translation="MSTTAQEKIDAVQGGAESWSAEDVVRWGLAEFHPHAAIASSFGA
                     EDVVLIDIAARVRADIRVFTLDTDFLFAETYELIDKIEKKYGIRVERVKSLYTPVQQA
                     EKVAPKLWTIDPNQCCALRKVEPLKAKLATLTAWVTGIRRDQAATRASAKKIEWDKSF
                     GLVKCNPLADWTWDRVWEYVREHEVPYNPLHEQNYPSIGCVQCTRQVQPGEDPRAGRW
                     SGFAKKECGLHAPE"
     gene            complement(25990..26286)
                     /locus_tag="VG272_03850"
     CDS             complement(25990..26286)
                     /locus_tag="VG272_03850"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWF12845.1"
                     /translation="MKITVEIPTALRRFTDGVQMLECNSDSLPELLGEIEQRFPAITK
                     HLRDDSGEIRRFINIYVNEEDIRFLGGNGYKFREGDRVLVVPSIAGGAPADACR"
     gene            complement(26380..27630)
                     /gene="thrC"
                     /locus_tag="VG272_03855"
     CDS             complement(26380..27630)
                     /gene="thrC"
                     /locus_tag="VG272_03855"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HWF12846.1"
                     /translation="MAEHYQLKCRECGKLWGNQARSFCDECLSPLEVIYDLEAIRPLF
                     TRKNIEAGPTNLWRYSALLPLPEDYKATIPTGFTPLVQARRLGKQLNTTNLYLKNDAV
                     CFPTLSFKDRVVATALAAARRFGFQVVSCSSTGNLANAVAAQAAREGFDAWIFIPSDL
                     EAAKVTGTQVFGAKLVRIAGNYDQVNRLCSQIAEKYRWGFVNVNLRPYYAEGSKTVGY
                     EIAEQLGWRLPDNIVCPMAGGSLITKIRKSFNELIALGLVEEKKVKFFGAQATGCSPI
                     SDAVKRKLERFEPQKPQTIARSLAIGNPADGFYAARAIRESGGWAEDISDPEIVKGIQ
                     LLAETEGIFTETAGGVTTGVTRKLLEQGYINPSETTVACITGNGLKTIDAIQEEYTLT
                     PAIEPRMTVFENFINDRMVAVAGN"
     gene            28143..29660
                     /locus_tag="VG272_03860"
     CDS             28143..29660
                     /locus_tag="VG272_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12847.1"
                     /translation="MKPYLLVFFAIQLAIACYAQDQHTDSFSQHKGTKVSRAKSHPSG
                     SNDILAVDGVKYKTLASALAACALPGCVVYDNYPETFAANPFASLPSNVFAEVHLLRR
                     TWITNVSIVVPNKSLLLGSGRGDAGSSGTVIQSGPMLSPATPVLQMGSAPIAMGVRIE
                     NLTVDCNNKIGATGIQNKWSQEESGLRHVLIVNCPVSGLDVETSAAQNSGPYEDLEVL
                     NYEICSNCSAATVPIIVKEVTAFRGIHGATINSDGARSPGAGIQIDSSGSFTDIHCEH
                     VDSCLLIGSQRATSAVTASNIECGPATLECVIFSNAYESRNLTALGIVATSGNLVADQ
                     ISDKIVSASAEGGSLGMYSVGSGNAHTLITSSPHLNSRFPGVAFLGSVSGSITHLAPP
                     AAGTNTITDPPATGTASLAAVENCGANQGETQDCRRSVETLPIIIWGEANLNSAVSQS
                     ITKLPFNDTLFSCTGSDETTSSGIVSFVDYKRDSVVIRETNGTTTDRLRYICVGH"
BASE COUNT         6672 a         8189 c         8087 g         7189 t
ORIGIN      
        1 cttgcgtgtg ccgaatcatg tggctgatga gctccacggt cagcagcgcc gagccaactt
       61 ccacaacgaa catgacaggg ttcttcatga gcgttgcagg attcagtttc acgaacgaat
      121 cccctacggc tcgccgtgcg atgggtccgt cccagattga tttgctcttt cttgcttggg
      181 ctgtcatttg tttttcctta gaaagtcttt ccggcatgca tcaacaattg ctcgagaatg
      241 gggccgaggc tgagcgccgg gaaaaaagta agtgcaccga caatcacgat cactgaaatc
      301 aataagcctg tgaaaagagg agaagtaacc gggaacgtgc cggacgtttc cggaacatgt
      361 ttcttttgca ccaggcttcc ggcgattgcc agcaccggaa tcaccatgaa gaagcggccg
      421 aacagcatgg tcgtggcgat ggttgtgttg taccagagtg tattggcgtt gaggccggcg
      481 aaagcagagc cgttattcgc gcctgcctcg gcgtaggcat aaaagatttc tgaaaggcca
      541 tgcggaccgg cgtttgcaag actggttaaa ccaaatttgg gcgccacaag tgaaatggcc
      601 gtgaaggtca ggaccatcag cggataaatc agaaccgtga gcatggccat cttcacgtcg
      661 aaggcttcga ttttctttcc gagatattcc ggcgtgcggc ccaccatgag tccggcgata
      721 aatacggaaa gaataatgaa aacgacgatt ccgtagatac cggaacctac tcctccgaag
      781 acgacctcgc ccaggaggat gttgatgagc ggaatcattc caccgagtgg cgtgaacgag
      841 tcatgcatgg aattcacggc gccgcagctt gtgtccgtgg tgacgacggc ccaaagcgcg
      901 gagttcgcga ttccgaagcg gacctcttta ccttccatgt tgcctgtcga tgcagtttgt
      961 gttgctgtgc tctgtaagag cgggttgcca cgagcctccg cggaatacgc cgcagtgact
     1021 ccgacgagaa acaggatggc catcgcacac catgcggccc aaccgtggcg cggcgacccc
     1081 gtcatttgtc cgagcgtgta ggtgagcgcg gagccgatgg cgaaaatcaa aaccagctcg
     1141 aagaaatttg tgagcggcgt tggattttca tacggatgcg cgctgttggc gttgaaaaag
     1201 ccgccgccat ttgtcccgag ttctttaatc atttcctggg aggcaacggg accctgagcg
     1261 atggtctgct ctttgacgat ctgcgtggtc gtttttccgc tcgcgtccgt tgtctgcacg
     1321 atttgcggat ccatgagttc cgcagtcgcg tagggcttta ggttttgcgg cactccctgc
     1381 cagacgagca gaagcgcgcc gagcacgcag ctggggagaa gtacccagaa agttgcgcgc
     1441 gtcatgtcca cccagaagtt gccgagcgtc ttttgctcgc ggcggacgat gccacgaata
     1501 atggcgatgg ccaaagcgat tcccaccgct gcagaaataa aattgtgata ggcgaggcct
     1561 gccatctgcg tcaagtaaga catggtcgtt tcagggacat aggcctgcca gttggtgttc
     1621 gtggtaaaag acgcggccgt gttgaaggcc agggccgggt ccacggcggc gagtttctgc
     1681 gggttgagag ggagatggtt ctgcagccgc tggatcagat acaggaccac catcgtgacg
     1741 ccgctgaacg tcagcatcgc gccggcatat tcggtccagc ccatctcttt tgtttcatcg
     1801 acgcccgtca gcccgtatac gagcttttca accgggcgca tgaggggatc gagaaatgtt
     1861 ttctcccggt tgaacgcgcg cgtcatgaac atgcccagcg gctttgtgag cgcgagcacg
     1921 acgagcagaa aaaatatgat ttggaaccat ccgttgtttg tcatggtcag aacttttccg
     1981 ggcgcaacat tgcgtaaacc agatagacga gcaatgcggc gcacactacg agagatagaa
     2041 tcgtatcgag catttttctt acctcatccg actacaaaaa tttacgtaca gcacggacaa
     2101 tgcgaaaaat acaaaggtgg agatcgtaaa aactgtgtcc agcattgcag ttcttccttt
     2161 ccttttcttt cctgggtttc aggtggcccg taatacttta tttgcagcct tggctcgctc
     2221 ttggttcaga aaccacgcgt ctccgcattt caattgtttt ggcaattcga cttaggtgct
     2281 cgggagaaaa attgatttgc ggatagtgag cagggaaagg gattaccgag ttctgattct
     2341 tgggataaag aaaaatcttg ggtataacgg caatcctatc gcgcaatttc ttctcattaa
     2401 gctgagtttg gatgggggat tggcggccgc cattataccc gcgccattca ttccagaaat
     2461 taacaagagc atagatcagg aaaaccaggc cgatgcaatc aacgaataga aaaatgtcta
     2521 tggccattgg atgtcctccc atcgacagcc ggaaagtgcg tggttggagc atcgtgtcct
     2581 gctcgtttca cccaccgcga ggtggcatcc agataccact tgtacgcgtt tgccgtccct
     2641 gacatctgtg cgcttcatta cgcttcccat tcttctaacc tgcttcgggt tgctgtcatg
     2701 caagagaatc atgcgatttc gggagttaag aagtcgtgcg caggttgtaa ggattttgta
     2761 aaacggaaac agttgatttg gcacgcttgt taaggcgatc tagtgcccct acaataccaa
     2821 ttgcaatact tatgttttcg acccacaaaa aacgaaagtt agagatgctc acggtattgc
     2881 tggctctggc attcctttgc gtgagcaatg cgcgcgcgca ggaacgcgcg gattgccgag
     2941 tgattcacag cgttattctg cattctcccg ttcggttctg cgtatatttg cctgccagtt
     3001 attccgagcc cggtgcgaag acgcggaaat atccagtgct ctatctgctc catggacttg
     3061 gcggcacgca gcaatcgatg gccgtaaacg gggagtggac cgaacttcag gatttgcgcc
     3121 gcgaaaataa ggtgggggat tttattgtcg tttcgccgga ggcctgggat acgttttata
     3181 ttaattcgcg tgatggccaa acgccttaca gtgatttttt cttgcgtgag ttcatgccct
     3241 atgtcgagcg gatttaccgg gttcaaggca ccagaattac gcgagggatt acaggatttt
     3301 ccatgggcgg ttacggtgcc ttacggtttg cgtttttgca cccggaactt tttggttccg
     3361 tgagcgcgca tagtgctgca atcgaacgag atccgcccaa tccggtcgaa gggatccctt
     3421 ctcccggcaa ttttgcttcc gaaattcttg gaaaaacgtt tggcgatcca attaacaagc
     3481 aattttggtt tcagaacagt ccctacgtat tggccaggaa aaatgcagcc gagctgagga
     3541 gaatgaaaat atattttgat tgcggtacgg aagataccta tgggctcgaa gctggagcta
     3601 ccgaaatgca cgaagctctc agcgccctta aaattccgca tgaatttcat ctttatccgg
     3661 gagggcacac cggatcttat ctggtggagc atcgctccgc atcattcgaa tttcactggc
     3721 acgtatttca ctcggctctc gacgccgcaa agtaagtttc atcgcaaaaa gctcccgact
     3781 cacggaatgt tccgcttcca ggaatatttc tcgacaatat tgtcattatt agtgacaatg
     3841 ctgtatattg gccccgcgcg ggaagccatt ctcttgccat atcagctcag cacgaaagtg
     3901 tttttcgggt ccgggtgtgt gatttccgga acgggcttac gtgttggttg atttgttctt
     3961 ttcggacttc tcccgtgtcc gcgagggcgt ttgttcctgc tttcgccggt tagttgttgg
     4021 gaggcgctcg tgcacacatt ccattctata ttcatttacc tgctagtgaa cccttcgaag
     4081 agcgtattta cgcgattcag gcttgtttcg attgttctgg cggccgtgat ctgcatggca
     4141 gtgccgttga aattgtttgc cggtgtatcg gtcgcggttt cgcccgcctc ggtcaatctt
     4201 tcaccaaatg gggctcagca gtttacggcc acggttacgg gcgcgagcaa cacttccgtc
     4261 acctggagta ttcagcaagg agctacaggc ggaacaatcc tcggctcagg actctacaca
     4321 gcccccggtg ctgtgggtaa ttacgttgtc atagttacca gcaacgcgga taacacgcag
     4381 agcgcttttg caacggtatc cgtggccgga ttcatccatg cgggattgct gaatcccgat
     4441 ccggtaacgg caactttact ggcgagtggg atggtgcttt acacggatgg cggaccgtct
     4501 ggttttgtaa cgagcccggc ggaaatttat aatccagcaa cttctacttc aaacgcgact
     4561 gggagcatga cgatacttcg ctggctcgaa acggcaacgc tgcttggaaa tgggaaggtt
     4621 ttatttgcag gcggtcagac atcgagcgga acgactgcca cggctgaact ctatgacccg
     4681 gttacaggga tgtttgcggc tacaggcagc atgactgttc cgaggcaagg ccacacggca
     4741 acgctactgc cggacgggaa agtgctgatt gtgggaggag tggtgggaaa ttgcaattcg
     4801 tcctgctctt acaataccgc tgagatttac gatcctgctg ccggtacttt cagtctgact
     4861 gcaggaaact tgccaagtcc tccaggcagc gtagcgtcaa ttctcttact cacgggcaaa
     4921 gtgttggttg caggcggagg aagtggcggg tccgccgagc tgtacgatcc cggcacaggt
     4981 ttttttacgg caacgggaac attggttaat caaaacgatg ctttttccgc aacacttttg
     5041 cagagcggaa aggtgctctt cgttacgggc ggaaataacg gggtagctgc atcgactgcg
     5101 gagatttacg atccgtcctc aggaactttc acggcaactg gaaatctcca actcgctcgc
     5161 ggtttcgata cagcaacatt gctcaaaagc ggtcaggttt tgattgttgg cggactttcc
     5221 tcagctcccg cggaattatt cgatccggcc acgggaacgt tcagtctgac gggaagcgtg
     5281 caagaaatgc ggattagcca acaggcgacc gcgcttccgg acggaacggt tttaatcgcg
     5341 ggagggagca gtgtttttca ggggttaagc agcatcgaag tttacgatcc gtcagcgaaa
     5401 agttttagcg gtcaaagtgt gtttatgaag gcggcgcggt ctggtcatgc cgcggcgcag
     5461 ctggcggatg ggcggctgtt gctgaccggc ggggaagacg catttttaaa cgtgatttcc
     5521 agcgccgaaa tttatgatcc tgtaagcggt ggttttactc ttaccgggcc tcttatccag
     5581 ggacgacacg ggcatacagc tactctgctg ggcaatggaa acgtgctgat tgtaggtggc
     5641 tacgaggata gcgctggcgc tcagaacgga aatactctcg tcgcgaccgc ggagatttac
     5701 aatcccgcgt ctggaacatt cagcctgatt tcgggtccaa atttggcgcg tgcctatcac
     5761 acggcaacac ttttgccgaa cgggaaagta ttgatcgccg ggggtgtaac tgctgcccag
     5821 ttacaatcgc ccacatcgag cgtcgagatt tatgatcctg tcatgcaagc atttactcag
     5881 gcaggaaaca tgttcgcggt caggtacaac cacgcggcga cattgatgaa tgatggacgc
     5941 gttttgattt cggatggact tacgattgcc ggcactccag gaaacggaat cgggcttgac
     6001 gaaatatacg atccgagcag tggacaattc gcgcaagcgg ggccaaagga ggtcatcaac
     6061 aatcattcgg cggcgccgac cgcttcgatt cttttacaaa atggccaggt ccttgccgac
     6121 aatcaatcga tcttcgatcc ggtgtcaaat tcgcttacga cgctttcttc gctggtaact
     6181 ctacagacgc ttctacaaga ttatgagttt gcgctcctcc ctggcgggca ggtgctgaca
     6241 acgagcaatg gctacgctac ttatttgttc gatcccactt cgcaaacgta ttcgcagtcc
     6301 ggctcgatgc agtacttccg aagccgccca actctctaca cacttcccaa tcatgaagtc
     6361 atggttgcgg gaggcgtagg tattgcgcag gtggaaatct atgttcctcc cacggccagc
     6421 gccaatgccg cacccgctct ttcggcaatt aacccatcaa cggttgttgc cggcggagct
     6481 ggctttacaa tgctggtaga gggttccaac tttgtcagca attcggtcat caattttaat
     6541 ggagcagcgc ggcagacaac tttcctatcc accacacaac tcagcactgc acttttgccg
     6601 agcgacattg ccaacacagg gactgcaacg atcacggtgt ccaatcccgc gagcggttct
     6661 gccacaggaa ttacatcgaa tcctcttaca ctgagtattt tagcggcgaa cattcaaccg
     6721 gttatcggcg cattatcccc ggccagcaca actgcgggcg gcgccgcttt catactttta
     6781 ttgtcgggca gcgggttcac tcaaaattcg gcggtgacat ttaacggaaa ccacgtgcct
     6841 gccgctttca tcagcgtcac tcagttgcag gcgaacattc cggccggcgc gattgtcact
     6901 gcaggcagtt tcctggtggc tgtggggaat ccgggaggga atccgacaac ggtggtgagc
     6961 tttacggtaa acaatccggt gccacaggaa aatcttctct cgcccgcaag cgcgacggcg
     7021 ggcagcgcgg gcgtgacgct cgatgtaaat ggatccaatt tcaatttgtc gtcgaaggtg
     7081 ctgattaatg gagccgtgca cacaaccacc tacgtgagct cgacgctcct gcaaacaggc
     7141 ctttccgcaa gcgatttatt gaagggggcg acactcaata tctcggtaag caatccaact
     7201 cctggaggcg ggacaacgtc aatgctgcca tttgcggtaa ccgactattc tgtcgcagta
     7261 ccggttccaa ccgtgacggt cagtgcggga cagacagccg tattcaatct gaccgtcgcg
     7321 ccctcgaacg gaacctttgc aaactcgatc ttatttacgg ccacaggctt gccatcgggt
     7381 gcggtggcat ctttttcgcc gtccgccacc gtaacgccgg gtgcaacttc ccaaactgtg
     7441 attctctcga ttacgaccat cgctcatgct ttttccacca cggcatattt cccgcgattg
     7501 gctcggccga ctgtaatgtg cctaagtgtt atcggaattg catttgcgtt agtgggattg
     7561 gtttttattt catcgaaaac tgcagcattg caatggaagc gattcgcccc aagacttatt
     7621 accgcgatcc tgttgatcgg cgttgcaagt acgatggctt gctctaccgg aggcggagcc
     7681 tccgcaccgc aagcaaatcc tgcaagcgga actccagcag ggagttacac gattacaatt
     7741 accggggcga ccggcgggat cgtgcattcc gcatcagcga caatcaccgt catctgaccg
     7801 ctgtaacaaa gcttaacgac attttttccc ttgggtctga aaaaagtgaa gctggcagca
     7861 gctgtgcaat tacggtgacc gattgaatac aagtgtcata tctggtgata tttgtacatt
     7921 gttaatgaat ctgcctttag agtacgatta ttttacgcgg gagaatctat ccccacggat
     7981 ccgacgctga aacctttcga tggatggtct tgggcatcct cgattcggga tcatgcgcaa
     8041 agtcaacggt cctccccgac ctttcggatt tgacccgcgc tcatcacgga caattttgag
     8101 tccggctgac ggctgcgcgg gaggacttcg tgcgtaccat cttcgctgtt ctcgtctctt
     8161 tttttatttg tgctgtgttt tgccagccgc tcatggggca ggcacctccg cctccatttt
     8221 tatttctatt ggaagggaaa ggggttccca cgggcacggt gcacgctttc cgcatgaatt
     8281 cggccactgg cgggattgcc gaagttcccg gttctccatt caatgcgggc ctggttccga
     8341 atcaactgat tgtcgatcca acgggacgat tcgtttttgt gacgaatcaa gagtcggagg
     8401 atgtaactgc atttgcaatc gatcactcta ctggcgcatt ggcgcagctt ccaggttcgc
     8461 cttttttgat cggagcgcaa cccgttaccg ccgctgttga tcctaccggg cgattttttt
     8521 atgtttttgc gacaagcaat gtgtatggag cggatcagga gctactttac gaatacacga
     8581 ttgacgccat cacgggtgtt ttgaccctta gtagtccctc tcccactaca tgggaattcg
     8641 ggcaagggat actaatttcc aacattacat tcgattccgg ggggaattac gcatatcttg
     8701 ggcaaataca aagcggcatg ttcggagcgc ccactctaat ttgtgcggtt gattttggca
     8761 gcggaaattt gtccatcacg ggttctgtgc agcctgccaa caatggcgaa gcaaactaca
     8821 tcgcggtatc accgacaggc tcatttttat attcgggtaa tgctgctggg aatgttgcta
     8881 atgcatttgc aatttcggcg ataggaggtc agctcacaga aatttcagga tcaccctatc
     8941 tgatgctgaa cgctccctcg tcgcttgtcg ttcatccttc aggaaatttt ctgtacattg
     9001 caaacgagaa tcaaatttat caaaccacgg aacaacccag ccaatacaac gggagcgttt
     9061 cagcgttttc aatcaatgcc gtggatggaa ggctcatgca agtttcaggt tcgccattcg
     9121 ctgcgggcat taaccctgta tcggttatca cggaccccac cgggcgattt gtgtattcgg
     9181 cttcctctat gtatacgggc ggttttacga gcttcgcgca aatcatgggc ttctctgtca
     9241 atgtttccac cggagcgctc acaccatttt catcgcctgc atttaccgat tctgtgaatg
     9301 gcaatggcgc tcaattagct atttccaatg gcttgctacc cacaaatccg gtgcccatga
     9361 tttcgtcgct gaatccacct tcatccatcg ccaccgacac tgcgttcaca ctgcaagtaa
     9421 acggcgcgaa ttttgtgcca ggggccgctg tgtattttgg cggacaattt cggagcacga
     9481 catttgtaag ttcaacgcaa ctcaatgtga gcattctagc aagcgatgtc gataacaatg
     9541 gaaacatcgt cgtttttgta tttaatccgc tgccgggtgg cggagcttcc acgtcggttg
     9601 aatttccggt gtctgcactc gttccaacta tttcaactac aaatccaaca agcgttattg
     9661 ccatcccaaa cgtctttgat ttcttctgca taggcacaaa ctacaccgcc agttccgtca
     9721 ttaattttaa cggcatgccg ctatttactc tgtatgtcgg gccgactttg gtcctcggca
     9781 gtgctcctgc gggcgatgtg gctgctccgg gaatcgtcac aattgaggtt gtcactcctt
     9841 ctaacggagt gcccggcggc gggacttcca atccagtgac tatctcgatc cttccgcctt
     9901 tcgttcagct ttcggtttcg gatatttctc ctaccagtgc gacagcagga gggccgggct
     9961 tcgttcttac cgtaactgga agcggattcg ttccaagttc gcaggtgagc ttcaacctgt
    10021 taaatgttcc cacgacggtg attacttcca ctcagctgat tgccacgatc ccggcaagcg
    10081 cgattgcagc cgccggaaat ccatacgtca ttgttacgaa ccccggaggc gcaacttccg
    10141 cggcatcgac tttcacggtg aataatccac ttccggggac aggagcgatc attccgcaga
    10201 gcctcccggc tggaagcaat gcactcacgc tgaacgtaac cggcacaaat ttcactccaa
    10261 attccgttgt tctcgtaaat ggtgtggcgc gttccacaac gtacatgact tccacgctgc
    10321 tgctggcaac tctgctacca gctgacctca tgcacagcgc gacactgaat atcaccgttt
    10381 caaacccgcc gccgggtggc ggcactactg cggcgctggc gctttccgtt gctgactaca
    10441 atgtgagcgc cgtttctact gcccagacct taacggccgg ttcgcaagcc aactacaatc
    10501 tgatgatttc ggcagtgaat ggcacgcttg gtcatgcaat cgtcttcacg gcttccggcc
    10561 tccctccggg gacaagcggt actttttccc ccgcgagcgt gcctgcagga ggcggcagct
    10621 cgaatgtaat gttttcagtt accacggcgt cgcgttcttc gagtgcctat ttgcgatttg
    10681 attcctctgc aagatccaag tggcctttct ttgcggggat attgttttcg ctagctgcga
    10741 tgtggatttg gtttccattg cgcaatggga gaatgcgtct cgcaactcca ctatttcttg
    10801 tttcgttgct cctttgcgtt ttgttgagcc tcacggcttg cggaagcatg cttatttcgc
    10861 ccccggccca aatcaataca ggaacacctt ccggaaccta catgattaat cttgctgcta
    10921 catctagcaa cattacgatc aacacgcaga tcacgctaac gattatgtaa taatagttac
    10981 gtgttctgct ccgggcgaat tggattgaaa aagagattta gaggaagcaa gtgttccagt
    11041 ttgggttgcc gaaaattcag tttttactct cgccggatat ttcggacatt tattttcatt
    11101 gccatcctgc tgtgtaacca aaccattcgt gcacaggagt cgcctccaaa tccgccgccg
    11161 ggcgctccca cagagctgcc cggacaagtg gtattgaatg ctgccggcca gtgcgtccag
    11221 cctcctccca tggttcaatt gcgggattac aacggaccgt ttcacaaagt gattggcact
    11281 tttacgcaaa aactcgatcg ccagtcggtg cctgacccgc attacaaaca aggcgcgctg
    11341 ctttgctccc tcaaagtaaa agataaactc tttttgtacc tgcacgatac ggttgatcca
    11401 gaaactttta tcagcgcagg atttaacgcg gggatttcgc aggcggaaaa tgatgatcct
    11461 tccttcgggc agggaggaaa aggttacgca caacgtctgg gcgcgagtta tgtggatcag
    11521 gttcaggtgc gattctttaa ggaattcgcg tatcccataa ttttttccga agatccgcgg
    11581 tattaccgga tggggcaggg gccaccaaag aagcgcttcc tgcatgccgt ggcgcacgcg
    11641 gtggtggcat acaacgacaa gggcaggccg atgtttaatt tttcagaatg gctgggagaa
    11701 atcagcggag cctcgatcag taatctgtat catcccggaa acgaacgagg cgtgttgcca
    11761 gccgcgcaaa acatcgcgat cgatgttgca ttggatattg gattcgatga attgaaagag
    11821 ttttggccgg agaccgcccg gaaacttcgg ctgcccttcc gcgatcagaa cgtgccgcag
    11881 cccgtagcgg atccagccgc gagataattt cattcagtaa caaaaatccg aagttggtgt
    11941 gtagggttta gcggcgcggg ctttaacctg agggcgtatg gaccttgcaa ggactaatcc
    12001 ccgcagactc tgaagccccg cgccacaaat gcaaactttt tacgccagct tgccggtgcg
    12061 cgcttccagc aatgtccagg cacgctctcc tactacttct ttccaatgag cgtagtacga
    12121 ggaaaggcgc gggcggaacg aagaagtgtc cgcatcgtta aatgtcatgc cttgcgtggc
    12181 aagctttgtg cggaattccg tgttgagcgc ggtcaattct tcgcgctgct ggcgcacaaa
    12241 tttaaccacg tttcgctcga tgatagcctg cacatcggca ggaaagcgtt cccagagttt
    12301 caagttcgcc atcaggttga atcccgacca gccgtgattg gtcatgctca catatttttg
    12361 cacttcgtaa agtttcagga attccgcgac agccagcgga ttcgtttgcg cttcagctgt
    12421 gcctgctttt aatgtctcgt ataatttgtt gaagtttgcc accacgggag aggcgcccat
    12481 cgctttccat gcttccgtgt aaataggcgc atccggagtg cgaattttta gtccctgtaa
    12541 atcatccacg gtgcgaatgg gacgggttgc gcacgtgatc tggtgcattc cattgtcgaa
    12601 gcagcctttg ggcagtccat agatgccttt ggccttcatc tcctgccgca ggtagtcgcc
    12661 cagttcgccg tccagcgatt caaaaatttg ggccgtggat cgaaaggcaa aaagcaaacc
    12721 ttgcacgttc gacgccggga caatatttcc aatcgacccg ccattcagcg tgaagaaatc
    12781 gacttcgccg ctgaccagca tgttcagcgc ggcggggtct cctccagcaa ttttgttgtt
    12841 gtccggtaag acctgaactt gcacgcgtcc gtttgtttcc gtttttacgg ctgcccacat
    12901 ttttgagagc cattgatcca gtggactatc ggccggctga ttgtggaact ggcgcagctc
    12961 gaaatccgcc gcgcgcgcac gaccggtcat gagcagcatg ccgcctgcgg ccagcagtcc
    13021 gtgcgtaaat tttcgtcgcg agattgtttt gtggatcacg aatttctcct gggcccaatt
    13081 agcgaatcaa ttgtagcgcc cgccttcagg cgggcatcgt cggttcgctg aggacgcccg
    13141 cctgaaggtg ggcgctacac aatcatttct gattatttca cggactggcc aaattatccc
    13201 gcttgacttg tggaccgccc gaaataaaaa ctgcgcgcgg tttgatcgtc ccatccgaca
    13261 ttgcaatgag agatagacca ttttgcgacc ttggtcggct cggtgttcgc cttacccaaa
    13321 gcagggcagg aaagacaata gctgccttcg tgtgttccga tccagatcag gtttcgatcg
    13381 aactcgacga aaacattttc agttgtgccg cgccaccacg atgcgtagtc attaatttcg
    13441 ccttctaggg gaggcacgaa ccacgcaatt tccttgggct gggtggggtt cgagatatca
    13501 aaaacgcgca ctccggcgtt gaaccaggca atgaccacga tggagggatt cgacgtgccg
    13561 ggcgcgagcc agcattgcgt gttgtgcgaa ccgaagcgac cgcgcgcgaa acagaaatcg
    13621 gtgtacggag cgtcggaagg cgcggcgggg cgaggaaaga atccgatgat cttcggattc
    13681 ttgggatctt ttacgtccac catatacggc gtgtgataat tttcgcggca atctgcttcc
    13741 agagcttcat gcacggccac gattttgttc atgagctgag gatgggccgc gtcagcaatc
    13801 accggatagc acgtgtgaaa tgggatggtg ccaaaggtgt caaattcgta ctgcatgtgc
    13861 ccatacggtt tgggattttt tatgtcccgc agatccatgg cataaaaacc gaaagcgccg
    13921 aagccgccgt atccaatcgt cccgccatcc tcgacgcgct tgggaacggt cagtgcgccg
    13981 tgatttccgg tccaggaagt cttgtcaccg gcaaaacggt attttttgta ttcttcttcc
    14041 tcacctaatt tctgaccggg gacccaccac cgcgaaactt cctgaacgtt tgcggggtcg
    14101 gacaagtcca caatcatgag cgcgttgcta tagggacgct ggtgattttc caggcgcagc
    14161 gtctcgtccc atccacaatc gaggtacgca tattttccgc cgtcataaaa attgtggtgg
    14221 gtgccgttgc cctttacgcc ggtgctgtat tcctgcagca gattcggctt ggtgggatcg
    14281 gtgacgtcgt aattgcggat tccgcggagg cctttgtagg cgagaacctt gtccctcaat
    14341 tccttgtcat attggccgtg cggatattga ggggtcgcgc tggtcagggg ctgcgccgcg
    14401 gtacacatca tgatccattt tttcaatttg gtgttgtaga ccaccgggcc gttgccctga
    14461 atcacgcgct ttttattcaa cagaacagga ttcttggctt cgcttatgtc gacgaaatct
    14521 ccgccggccg gcaacaatcg ctgtttgcct ttggcccaca tgaccataag cggctctcca
    14581 ccgctgatac ttgaacccga aatatgggag ataatttcca tgttgtggat atattgtttt
    14641 ttatcgatgt acgtcagcga acccagcggc agaggtattt caggagccgt attggtcgtg
    14701 taaagtcctt gcacctgcgc gccagcgccg agttttccag ccagcccagc cgctgtcgcg
    14761 cctacagccg cactcaccgc gctcttgaga agcgatcgcc tggtgaacgc tttgcgcgtt
    14821 ccatttactt ctcctgtatt ttctttcgct gaaagggaag gcttgcgaat tttagacatg
    14881 actctccttt actcgctgat ttttgctgtg ggcacaaatc atggaatgga acatgcctcg
    14941 gcttgtatgc cttcgcttat atgagcccgt gtgccgcgca tcatacccca atattctgcc
    15001 tgtgataaat ccgaaaaaaa accaagggct gagccggccg caacatgttg cgggtcggtc
    15061 agcccttctt aaattgtgat tctacttcca ggccgaatac accggcttcg ccagcagaaa
    15121 cgcgggattc gctccgggct ttggagtaaa tttctgaatg cggcctgcat cgacttcggc
    15181 cacgtacaga tttccttcct gatccacact gatgccgtgc actccccaga gataacctgg
    15241 atagtcgccc atgcttcccc agctatagag caggtgaccc tgcaaatcgt actcgatcat
    15301 tttgttggtg gtgcgatcga aggtccagat cgtgcttccg gtgccgatat acagcaagtg
    15361 tagactggaa ggcgcattgt caatcttcca ttcgtagaga tacttgccgt tctcatcgaa
    15421 cacctggatg cggtgattgt tgcggtcatt cacgaaaacg tggcgtgtct tctggtcgat
    15481 ggcgacgccg tgcacgttgc tgaaatatcc gggacgcgtt tccagtggct tgttatcacc
    15541 tccgcgccgt gggccaccct tgatgcccca ctgcatgagg aattttccct ctttgtcgaa
    15601 cttggcgata cgcgttcccg tgtatccgtc ggaaacgtag aacgtgccgt ccggcaacca
    15661 atcgatgtaa gtcggacgat taaagtggtt ggcatcctcg cccggaattt ccggcgtgcc
    15721 aatcgtttgc aggagtttgc tgccgtcgtg cgtgaaacgg taaatcgtgt ccatgttgtc
    15781 gtccacgatc cacacgttct tttccggatc gtaagggctg atatacacgg agtgaggccg
    15841 gcggaagagc ttgtcccact gcttccaggt ctcgatgatg tttccttggc cgtcataaac
    15901 gacaaggcag ttttcccact ttgcatcttt gccaagttcg cggtatggag gccctttgat
    15961 tccgagcttt tcggattttc cttcccagga cgtcagccat tcgcgggtgt cttgatccgt
    16021 cccgcccgtt ccgggcaatg aagccacggt ggcatctcgc cagaagcctg cgacaggata
    16081 gctaatgctg gggccggctt ccggcagcaa catcggtttg ggcgggacca tctttggcaa
    16141 ttcaccgcgg tagagagcgt aaatgcgatt cgggctttca gcgaagacgc tttcgcctgc
    16201 gccgtatgtc catttttcgt ttcccggcag ggtgctgata tctttcggcc agttttttac
    16261 cacttcatag ccgccactca gatcttctcc gccaaccgct ccgggaacgg cagcgaatcc
    16321 ggtaccgggc ttcgactgtc cttccacggg cgaaagggtg ccggaaattc tgacgccgat
    16381 cgtcatgccg atcactaaaa tcaagaacat caatccaatt tttttcattc gatccctccg
    16441 aaaaacgcca atgtaataga ggtgcaacgc aacccctgcg tttcaacctt tgccaaaaaa
    16501 taaatccacg ttcaagatga ggtaattttc gccgatgagc gagatgtatt ggtcaccacg
    16561 gattgacgcc ctggggggag cctataagag gcgtcattgg atgacaatat gtttgtgaag
    16621 gagcagggaa atacggtgcg ccattttcat tttgtcttta cggatggaat caatccaatt
    16681 tttccgggca gtgattctgc atttcgcaca ggttccgctt tgggctaaag tagtaatcta
    16741 ataagcccag gggatggtaa tccattccag gccacgccaa ggagtaagga tgagcgtaac
    16801 ggacaaaacc gcttccatgc aaaacacatc cacatcgccg atagatatgc tctgcatcaa
    16861 cacgatccgc acactttcca tggatgcagt gcaggccgcg aattcgggac atcccggaac
    16921 gcccatggcc ttggcacccc tcgcatattg tttgtggcag gaatttttgc gatacgatcc
    16981 gaacgatcca gtttggccga accgcgaccg gttcgttctc tccgcgggcc atgcatccat
    17041 gctgctgtat tccgtgctgc acctcgcggg tgtaaaagac gtcggcgaaa atctggaaat
    17101 actgacgggg ccctcgattc gtctcgaaga cattaaaaat ttcagacaat tgaatagccg
    17161 atgcccgggg catccggaat cgcatctgac cacgggcgtt gagaccacaa ctggaccgct
    17221 cggacaaggc tatgcgaata gcgtgggcat ggcgctcgcc ggaaaatggc tcgcagctcg
    17281 atacaaccag ccgggatttg acgaagtcta caactacaac gtgtacgcgc tgggcagcga
    17341 tggctgcatg atggaaggaa tttcaagcga agccgcttcg cttgcggggc acctgagact
    17401 ttccaacctt tgctggattt acgattgcaa tcgcatcacg atcgaaggtg gcacgccgct
    17461 ggctttcgat gaagacgtgg ccgtccgctt tgccggatat ggctggaacg taacgcacgt
    17521 tacagatgcc aatgacctgg acatgatccg caccgggttc cgcacgttcc tcgccactaa
    17581 agatcggcct actctgatca tcgtgaacag ccacattgga tatggctcac cgaacaagca
    17641 cgacagcaat gccgcgcacg gcgaagcgct gggtgaggaa gaagttaaac tgaccaagcg
    17701 tttttacggc tggcccgagg acgcaaaatt tttgattccc gatggtgtgt acgaacattt
    17761 tcaaaagggc attggcacgc gcggcaaagc ggagcgcgat cgctggactg cgaaacagat
    17821 cgaataccgc gtcgcccatg ccgatttggc gaacgaaatc aagctgatgc aataccgcga
    17881 gcttccgagc ggctgggata aggatgtgcc ggtatttccc gcggatgcga agggtcttgc
    17941 cacgcgcgat tcctcgaaca aagtggaaaa tgcgattgcc aaaaatgttc cgtggctgat
    18001 cggaggctcg ggagacctcg caccttcgac caaaacgttg ctcacttttg aggacaccaa
    18061 agggttccag gctggcaatt atggactacg gaatttgcac ttcggcatac gcgaacatgc
    18121 catgggtgga gtgatcaacg gcatcgcgca gaccaaagtg cgcgcgttcg gcgccacatt
    18181 ctttgtgttc accgactaca tgcgcggggc catgcggctc agcgcaatca tgggaatgcc
    18241 cgtgatttac gttttgactc acgattccct gggcgtcggc gaagatggcc ccacgcacca
    18301 gcccatcgag catctcgcaa ttgcacgcgc tacgccaggc atgttgacac tgcgccctgc
    18361 ggacgcgaat gaagtggccg aagcgtggaa agttgtgatg cagttgcagc ataagcctgc
    18421 gcttctggta ctaacgcggc aaactgttcc tacgctcgat cgcacgaaat acgctcccgc
    18481 ttcaggcctt gcccgtggcg cttatgtgct agccgaaaaa ccgggcataa ctcccgatgt
    18541 gcttttgctc gccacgggca gcgaagtttc gctgtgcatc gaagcgtttg aacaactatc
    18601 gaaagaaggc attcaggcgc gtgttgtcag cttgccctcg tgggaattgt ttgagaagca
    18661 ggatcaggcc taccgcgatt ctgtgctgcc gccggccgtg aaggcacgcg tttcggttga
    18721 gatggctact acgtttggtt gggagcgcta tgttggaacg tctggataca tgatcggcat
    18781 gcacagcttt ggtgcttccg ctccgatcaa ggctctgcag aaacatttcg gcttcacggt
    18841 tgagaacgtt gtcgcagcgg cgaaggaaca agttcagtca tcgaaaaaat aagaatttag
    18901 aagcgagggc ttcagcctgc ggaatttagg cctcgcaagg accaaactcc gcaccctgaa
    18961 gcccgcgcta cttcagccca ggagaactca tggacgcgtc cgagctttgg aaacgctacc
    19021 gccagtatca ttgccacgtg gattcgctcg acctctccct cgacatcagc cgcatgcatt
    19081 ttgacgacgc gtttcttgcg tcgatggaac ccgcgatgca aaaagcgttc caggaaatgg
    19141 acgcacttga aagcggcgcg attgccaatc cggatgaaaa gcgaatggtt ggtcattact
    19201 ggctgcgcac gccgcagctc gcgcccacaa aagagattgc cctggaaatc gcgggcaccg
    19261 tggaatcgat ccagaaattc gctgctgatg tgcacgcagg aaaaatcagg ccgcgaaacg
    19321 cagagcgctt cacacaagtg cttgcgattg gcattggcgg ttcggcgctg ggcccgatgt
    19381 ttgttggcga tgcgctgggc gggcccgctt cggatcgcat gacgatccat tttatcgaca
    19441 acacggaccc cgacggcatt gagcgcatga tcgatgtatt aaaagttctt ctgcctgaga
    19501 cgctcgtcat cgtcaccagt aagagcggcg gaactcctga cacgcgaaac ggcatgctgg
    19561 tggtcgccga agcttatcgt gccgcgcatc tgaattttgc ggaacacgcg gttgccgtca
    19621 ccatggcggg atcacatctc gataaactcg cggcttcgga agcatggctg gcacgcttcc
    19681 cgatgttcga ttgggtgggc ggacgtacga gtgagctatc cgcggtgggc ttgctgcccg
    19741 cggcgctaca gggaatcgac attgtacgta tgctcgccgg agcggcggcc tgcgacgtgg
    19801 ccacgcgcat tcatgaaacg cggcgtaatc ccgcagcgct catggcgctg atgtggtacg
    19861 tagtgaccga cggcaaagga aagaaagaca tggttgtcct tccttacaaa gatcgactcc
    19921 ttcttttcag ccgatatttg cagcagttgg tgatggaatc gctcgggaaa caacttgacc
    19981 tggatggaaa gcgcgttgag cagggaatcg cggtgtacgg gaataaaggc tcgacggacc
    20041 agcacgcctt tgtacagcaa ctgcgcgatg gcgtgaataa tttctttgtg acgtttgtgc
    20101 gcgtcctcga atccggcggc tccccgctcg aagtcgaacc gggcgttaca accggagatt
    20161 acctggacgg attttatatt ggcacgcgtg aagcgttgta cgggaatgat cgccagtcca
    20221 tgaccattac ggtgcatcgc gtggatgcgc ggacgatcgg cgcactgatc gcgctctttg
    20281 agcgcgccgt gggtctctac gcatcgctaa ttcacatcaa tgcgtatcac cagccgggcg
    20341 tagaggcggg gaagaaagcc gcggcagccg tccttagcct gcaggcaaaa actcacgccg
    20401 cactttccgg catgccgcaa acggccacgc aaatcgcagc cgcaatcggc gccgcggaat
    20461 ccgcggaaag tgtctatctg atcctcgaac atttatccgc gaatggccgc gcacgattgg
    20521 cgcccgctgc caatccgggc aacgcaacgt ttacgcgtgc ctgaccgaaa catagaaaag
    20581 tcccggagga cgaaataacg tagcccacca cgcaagtggt gggggcgagg atcgaaataa
    20641 agaagccccg taggggcggc acacatgccc aaacaaatgt gccgcgccta cggggctgaa
    20701 tttttactgc gcgtgaatac ccaccacttc cgtggtgggc cacattattt cgttcctacg
    20761 ggacttcgct tacgacgtga cttgcgctgt cgtgcgcgca tcatcacttt tttcctgctt
    20821 gccgagtccg aaggcctggt gcagtgcctt cacggctttt tccattccac tcgattccac
    20881 gaggaacgaa atgttgtact cggacgatcc ttgcgcgatg gcgatgatgt tgatcgattc
    20941 cttgcccagt gtgctaaaaa cgcggcctgc gatgcccggc gtcccggcca tattttcgcc
    21001 gactgcagcg acgatggcca cattacggat gaccttgatg tgatcgagat catgctgatg
    21061 cacatcatca tgcaaggcgg cgcgcagagc ctcttccgta cgtgttgcgt cggctgcttg
    21121 caccacgaaa caaatgctgt cttgtgaaga ggattgcgaa atcatcacga tgtttgcgtg
    21181 ggccgaggcg gtcgccgaga aaatgcgcgc ggcgatgtcc ggcacgccga taattcccgg
    21241 cccggcaatg gcgatcatgg ccacatcgcg tgttgctgtg acagctttca cgccttgcgg
    21301 cgcgggcttt acggattgcg taattttcgt tccgacgaga tcgggatgga agctgtcgcg
    21361 aatccacaca ggaatcgcgc tttcgatcat ggggcgcaag gtcatgggat gaagaacttt
    21421 tgcgccaaag aaagcgagtt cgcccgcttc cgtgtaggaa acttcgtcga gcgtggcggc
    21481 gttgggaacc atgcgcgggt cagcggtcag cacgccgttt acgtctgtcc agataatcac
    21541 ttcgctggca tcgagcgctg cgcccaaaat tgttgccgag taatccgatc cgccgcgccc
    21601 aagcgtcgtt ggaattcctt ttgccgtggc ggcgacatac ccggtcacga cgggaattcc
    21661 gttcgcttcg agcaggggaa gcagcatgga ctgcgttttc gttcgtgtgg gctcggagag
    21721 gggacgcgct cctccgtgaa cgtcatccgt aacaattaat tcagttgcgt cgaaggcaac
    21781 gccgcgaagt cccatttcgc gcagtacagc cgcggtcatt cgcgcactga gtcgttcgcc
    21841 agttccggca atcacgtcaa gggcgcgagg agaaagttcg ccaagcagcg cacagccccg
    21901 gcaataattt gccgcgcgct caatcaatgc gtcgatttcg gaaagcagct gccggcggtt
    21961 ttccggatcg gaaatcagtt tgccaatcgt ttcgtgatgt ttttcctgga gtgacctggt
    22021 aagcgcttcg gctgctgctt catctcccgt tgcggattta ccagcggcag cgatcagcat
    22081 attggtcaca ccggacatgg cggaaaccac cacgactacg gcgcgatctt tcgaggcgtg
    22141 cgccgcaatc gtcgcagcgc tgtggattcg atcggcgcta ccgaccgacg ttccgccgaa
    22201 tttcattacc tgaatcggac ggctcatcgt accctcactt cggcaggagc cgcgttttcg
    22261 tttgccgatt tcgcatgcaa caacccgcgg gcgcatagca attccgcgtt caaaatggcc
    22321 gcacctgcag cgccgcgaac cgtattgtgg ccgaggacca caaagcgata atcgaacacg
    22381 ctgtccggaa aaagacgccc cacggtgacg ctcattccgc cgcccgcatc acgatccatg
    22441 cgcggttgtg gcctgtcctt ttcgccgcgt acaaggattg ggtgcttggg cgcggtgtgc
    22501 aatttcaatt cctgcggcac cgatgtatag gaagccagcg cttcgcgaac ttcctcgatg
    22561 ctcgcgcgct ttgcgagttt cacgcgcacg gcaaccatgt gaccgtcggc gacattcacg
    22621 cgatggcact gcgcgctcac cggaaatgct gcattctcaa aagaagaagt ttttccaatc
    22681 gtgccaagaa ttttgcgcgt ctcgctttcc attttcggct cttcgccgtc gatgaaggga
    22741 agcacgttgt ccaccacatc gagcgacggc actccggggt atcccgcgcc ggaaatggcc
    22801 tgcagcgtgg tggcaatcac tgcttcaatt ccaaatttat tgtgaagtgg cgcaagagcc
    22861 agcgccatga tgatcgtcga gcaattcgga tttgtaacga tgtacccagt cgagaatccg
    22921 cgctcacgct tttggcgctc cagaactttc aagtggtcgg aattaatttc gggaatcaag
    22981 aggggaacat cctcatccat gcggaaagag gaagcattgc taatgacggg ataacccgcg
    23041 cgcgcgaacg cttcttccga ggagcgggca acatccgacg gcaaactcga aacgaccaca
    23101 tcacaatcca gcggcggctg tggtggttgc acgaccatcg aacgggcgaa ggccggcatg
    23161 tcgcccacca gccgccaggt acacgcctcg ccataagttt ttccttgcga acgatccgac
    23221 gctgccagag ctgtgatctc aaaattcgga tggtgctcca gcatttgcac aaagcgctgg
    23281 ccgaccatcc cggtcgcacc taaaattcca actcgcactt tcttattcga cactgcccct
    23341 cccgtggaga tcatgatatg gatgctgttg agccagctgc aaaatttctt cgctcgcctt
    23401 gcccgaaaac gtaacgtcgc gcgcggcagt ttcgtctgcg tcgatgcccg cgacgatacc
    23461 caccaccagc agcgatggtg acgccgctgc cgggcattcc tgcaactttc caatcgtggt
    23521 gcggaaaatt gtttcgtcct tgctcgttgc gcgagaaatc aaagcgcagg gaatttccgc
    23581 tccaagtccc gccgcttgca gctccgcgga aagctccgcg tagcgatgtc ccggcatgaa
    23641 gatcacgtat gtcgtgtccg tcgcaatgtg tccctcgttg cgaagtccag tgccgccttc
    23701 gcgatgcgcc gtgacgaata cgacgcgcga tgcgcggcgc cggtccgtca gcgaaatttg
    23761 tgccgaagcc gcaccgccca gcgccgaagt aactcccgga attatttcaa agtctgtgcc
    23821 ggcggcacgc agcgcttcca tttcctcgcc tgcgcggcca aataacattg gatcaccgct
    23881 ttttagccga gcaactattt ttccttcgcg agccagctgc gccattagcg cattgatctc
    23941 gtcctgcgaa atgctttttg cgccgcagcg ttttcctacg ttcacgacct gcgtcgtcac
    24001 gggaaggacg cacaagatat cctgtggcac gagatcatcg tgcagcacga cgtccgccga
    24061 ctgtaaaacg cgccaagctt tgcgcgtcaa cagttccggg tcgccgggac cagcgcctac
    24121 aagataaact tttcccgcca ttttgctgaa gactccagat tatttccgac tccaacccaa
    24181 catctcgatt tatgtagcgc tcgccttcag gcaagcatgt gtgcctgctc ctaaaatgtc
    24241 cgcctgaagg cggacgctac aaaaaccaat gcaaaaaccc gcgccacttc aatgcggctt
    24301 cgggtccagg ctcgcatatt gatgcagcaa tcgtttgcgc tgctcgggcg gcattggaat
    24361 gctttttaat tcatcgcgcg tgcgtccgag tttcgcgatc cactcttccc actccgggcc
    24421 gaattgctct tcgagctgct tgcgcaaacg ctgggcaagt gcaggactca atccgccagt
    24481 ggaaatcgca atttgcaaat caccgcgccg cacgacggca gggaagtaaa aatcgcatcg
    24541 ctccggttca tccaccgcgt taaaaagtat gccgcggctt ttggcttccg cgtaaatttc
    24601 atcgtgcagc gtggtcgacc cagtcgccgc gatcgcaata aacacgcccg caaaatcaga
    24661 cggtttgtat tcccgcgctt cccactgaat gcgtccctcg cgcgcccatt cgcgcacacg
    24721 aggagtagct tgcggcgcga tcacgcgcac agtggcccct gacgccagca agctttcaat
    24781 ttttccttcg cctacagtcc cagcgcccac catcaggatt aggcgccctt cgagttttaa
    24841 gaacatggga aataaactca tcgcttttct cttttcaatt ccgaaccaaa atcgaatcgg
    24901 gcggttttca gtagcacaga cttcagcctg cgaaatttcg tccgtgcaag aactaaaatc
    24961 cacaggctga agcctgtgct actcaggcgc gtgcagtccg cattcttttt tcgcgaaacc
    25021 ggaccagcgg cccgcgcgtg gatcctctcc gggctgcacc tggcgagtgc actgcacgca
    25081 gccgatgctc ggataatttt gctcgtgcaa agggttgtaa gggacttcgt gctcgcgcac
    25141 atattcccac acgcgatccc atgtccaatc cgccagtgga ttgcatttca ccaggccaaa
    25201 tgatttatcc cactcgatct ttttagcact ggcgcgcgtg gccgcctgat ctcggcgaat
    25261 ccctgtgacc cacgcagtca gcgttgccag cttggccttc agcggttcca ctttgcgcaa
    25321 cgcgcagcac tgattcggat cgatggtcca aagcttcggc gcaacttttt cggcctgctg
    25381 cactggcgtg tacaaggatt tcacgcgctc gactcgaatg ccgtattttt tctcgatctt
    25441 gtcgatcaac tcgtacgtct cggcaaaaag aaaatccgta tccaaagtaa agacgcgaat
    25501 gtccgcccgc actcgcgcag cgatgtcgat aagaaccaca tcttccgcgc caaagctcga
    25561 agcgatagcg gcatgcggat gaaactccgc gaggccccag cgcaccacat cctccgcact
    25621 ccagctttcc gcgccgcctt gtaccgcgtc gattttttcc tgcgccgttg tactcatcgt
    25681 gccctcgtag ccgtaggaca ggcttcagcc tgtcctactt aaaaaacgga ccataaacaa
    25741 aaatgcccgc gagctttggg cttcgcgggc cacatttaag agctggaagg atgtcttact
    25801 acgcttgcaa atccttggct cccaaggggg accgcgccgc cttggtacac gtaggcgtgc
    25861 gcttggtaat cgtaattccc ttgagaatag acataatcat tacccttcca gcttaaatca
    25921 gccgccaaat ttgtcaagcc aaagttcctg tagccgtcgc gatcccgcgt gctaaaaaac
    25981 gtatctgctt catcggcaag catctgccgg cgctccgccc gcgatcgagg gcacaaccag
    26041 cacgcggtct ccctcgcgaa atttgtagcc gttcccgccg agaaagcgaa tatcttcctc
    26101 attgacgtaa atgttaatga aacgccggat ctcgccggaa tcgtcccgga gatgcttggt
    26161 gatggcgggg aagcgttgct caatttcgcc cagcaattcc ggaagagagt ccgaattgca
    26221 ttccagcatt tgcacgccgt ccgtaaatcg cctgagagct gtaggaattt cgacagtaat
    26281 tttcatttca attatttttc ctgatctgca atttttttga aacctgggaa tgccaatcgg
    26341 gtgacggacg ttcccagggc ccccgctaat ttcccaaaac tagtttcctg cgacggctac
    26401 catgcgatcg ttaatgaaat tttcaaacac agtcatgcgc ggctcgatgg caggagtgag
    26461 agtatattcc tcctgaatgg cgtcgatcgt cttcaagcca ttgcctgtga tgcacgcaac
    26521 cgtggtctca ctcggattga tatacccctg ctcgagcagt ttcctggtga cgccggtggt
    26581 cacgccgccc gccgtctccg tgaaaattcc ttcggtttcg gcgagcagtt gaatgccctt
    26641 gacgatctcc ggatcggaaa tatcttccgc ccagccgccg ctttcgcgaa tggcgcgcgc
    26701 ggcgtaaaag ccgtccgccg gattaccaat cgcgagcgaa cgcgcgattg tttggggttt
    26761 ttgcggctca aaccgttcca gcttgcgctt tacggcgtct gaaataggag agcagcccgt
    26821 ggcctgcgcg ccaaaaaatt tcactttctt ttcctcgacg aggccgagcg cgataagttc
    26881 attaaacgac ttccgaatct tggtgatgag cgagccgccg gccatcgggc aaacaatgtt
    26941 gtccggcaag cgccagccga gttgctcggc aatttcatag cccacggtct ttgagccttc
    27001 ggcgtaatag ggccgcagat tcacgttcac gaatccccag cggtattttt cagcgatctg
    27061 cgagcacaga cgattcactt gatcgtagtt gcccgcgatg cgcaccagct tcgcgccaaa
    27121 cacctgtgtg ccggtcacct ttgcagcttc gagatccgag ggaataaaaa tccatgcgtc
    27181 aaaaccttca cgcgcggctt gcgcggccac cgcgttggca agatttccag tcgatgagca
    27241 actcaccacc tggaagccaa accgccgagc tgcagccagc gccgtcgcca ccactcgatc
    27301 cttgaaggag agggtgggga agcaaacagc gtcgttcttc agataaagat tggtggtgtt
    27361 aagctgcttg cccagcctgc gcgcctgcac gagtggagtg aagcccgtcg ggattgtggc
    27421 tttataatct tctggaagag gaagcagagc gctgtagcgc cacagattcg tgggtccggc
    27481 ttcgatattt ttgcgggtaa acagggggcg aatcgcttcc agatcgtaaa tcacttcgag
    27541 aggcgagagg cattcgtcgc aaaacgagcg cgcctgattt ccccagagct ttccacattc
    27601 acggcatttc agctgataat gttcggccat ttggcctcct gcgatcgctc cgcatacatt
    27661 cagatggtgt agcgggagaa tcgattccag atttgtgact ggtaaactgc cgcaggttgg
    27721 gcggcgtgtg aggggtttcc taaaaagcaa aaaaccctcc acgccaaaga gggggagggc
    27781 tcaaaagact tagtctgagt gctcgtccga ctctttagca gttttttacg tggagcaagt
    27841 tgccttaaat cgccacgtgt ccagcaccca aaagtatagg gagtcgccga tggtgtgtca
    27901 agagctttca aactcgaacg aatgatttca tcggataggg gataggcagt cggataggca
    27961 gtccgccctt attttgtcct ccaaagagag cgagacgata tttcgccaga tctacgttgc
    28021 cccaaagagc gcctcattgc cgaaaggagc tatctccgca tccaacagaa aagagccgtt
    28081 ttggattcga ttgaaggtat tttgcaggaa aatcagactt taaaccacag tgggaaagac
    28141 acatgaaacc ttaccttttg gtattcttcg ccatacagtt agcgatcgca tgctatgccc
    28201 aagaccaaca tacagactca ttttctcagc acaaaggaac gaaagtcagt cgcgccaaat
    28261 ctcatccgtc cggttcgaac gacattctgg cggtggatgg cgttaagtac aaaacactgg
    28321 cttcagcgct tgcggcttgc gcgcttccag gatgcgtcgt gtatgacaat tatcccgaaa
    28381 catttgcggc caatcctttt gcttctcttc cttcgaacgt atttgcagag gttcatttgt
    28441 tgcgacggac gtggattacc aatgtcagca tagttgtgcc caataaatcc ctacttcttg
    28501 gatcgggacg tggtgacgct ggctcgtcgg gaacagtaat ccaatcaggg ccaatgcttt
    28561 cgcctgccac tccagttttg cagatgggca gcgcgcccat tgcaatgggc gtgcgcatcg
    28621 agaacctgac agtagattgc aataacaaga ttggcgcgac cggtatccaa aacaagtggt
    28681 cacaggaaga atccgggttg cgtcacgtgc tgatcgtgaa ctgcccggtt tccggattgg
    28741 acgtagaaac ctcagctgcg caaaatagcg gcccgtatga agacctggaa gtgctcaact
    28801 acgaaatatg ctcgaactgt tccgctgcga ccgtgccaat tatcgtgaag gaggtaaccg
    28861 cgtttcgggg aatccacggc gcgacaatta attctgatgg ggcaagatca cccggcgccg
    28921 gaattcaaat cgactcgtca ggatcgttta ccgacattca ttgcgagcat gtggattcct
    28981 gcctgctgat cggttcgcag cgagccacaa gcgcggtgac tgcctccaac atcgaatgcg
    29041 gccccgccac tctcgaatgc gtgatttttt ccaacgcgta cgaatcccgg aatctaacgg
    29101 cgctcggcat tgttgcaaca tcaggaaatc tggtggcgga tcaaattagc gacaaaatcg
    29161 tttccgcttc ggctgaaggc ggcagtttgg ggatgtattc cgtggggagt gggaacgcgc
    29221 atacgcttat caccagttcg ccgcacttga actccaggtt tcccggcgta gcgttccttg
    29281 gatcggtcag cggttcaatc acgcatctgg ctccgcccgc agccggaacg aataccatca
    29341 cggatccgcc ggcaaccggc acggcttcgc tcgcggccgt cgagaattgc ggagcgaacc
    29401 aaggagagac ccaagactgc agacgatcag ttgagactct tccgatcatt atttggggcg
    29461 aggcaaactt aaattcggca gtatcgcagt ccataacaaa actgccattc aacgacacgc
    29521 tgttttcctg caccggatca gacgaaacaa cttcatcagg aatcgtctcg ttcgttgact
    29581 ataagcggga ctccgtggtc atacgggaga cgaacggtac aactacagac cgcctccgct
    29641 acatttgtgt gggccattag tagtgcgggc ttcagcctac ggggttcagg ccttgcaaaa
    29701 aatagaacgc ttgaaatttt agcctttcat ggcctaaccc cccgcaggcc gaagtctacg
    29761 ccacttaagg ccgctccgcg catggctcga attatgcggc aggcgagtgt ggtgtcccaa
    29821 tttgaaaaga attcgcgtgg cgcggctcct taggaggtcg ggacttcagt cccgacgacg
    29881 agcttggttt gatcggggtt ttaacccctg aagcttcagg ggatgaagcc cgtgacgaaa
    29941 ggcaacccaa atagccgagt tgaaactcgg ccctcctaaa ttcgagaaat tttcaaatta
    30001 gagcatttct aatttaagta tgaacatctg tgcgataatg ctcctgcctt tcgtaggagg
    30061 agcggatgca agcgtattcg caggatttac gggaacgcgt gttgcgggcg ctggaacgtg
    30121 gggatggccc tgtggag
//
LOCUS       DASZMB010000081         9320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2556490, whole genome shotgun sequence.
ACCESSION   DASZMB010000081 DASZMB010000000
VERSION     DASZMB010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 9320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9320
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2556490"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VG272_03865"
     CDS             <1..364
                     /locus_tag="VG272_03865"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF12848.1"
                     /translation="TLDLFPRLKICAAHGGGYLPSYSSRGDQCLTAFPNLCKPLKKLP
                     SEYLKQLYFDSVLFSPEDIRHLVAVVGASQVVVGTDYPTLWNRTPVGRILTVPGLSDE
                     ERISIFSGTAAKLLKINL"
     gene            388..1293
                     /locus_tag="VG272_03870"
     CDS             388..1293
                     /locus_tag="VG272_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HWF12849.1"
                     /translation="MKQKVEARENRNRRNFLKAAGSVAAIGLAAANNSFAQSENSKSS
                     AHMERMKMAPPDRGMWITWYDLPTEGRDAYFSWLHGTYLPALLKRPGYLWAAHYASQD
                     LEGGDENAKRYHHVDDPKVGKGFHYMLLIAATDANAFGDPVPSVLHAVLPEKDKKMLA
                     IRTGERINIFTETGRRDGTANGAYKEGMLGTPCIQVGSFDCALEFEEEMHAGYVLERL
                     PKMCATPSCVRVRKLNSVVGWAKHGIIYEFSSKEGFDRDYAPAVAKSPLGLNGHSVVP
                     ELVHAPNGPNSALRIWPPVPKAGVA"
     gene            1400..2641
                     /locus_tag="VG272_03875"
     CDS             1400..2641
                     /locus_tag="VG272_03875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF12850.1"
                     /translation="MAATQTESLVDVGHEVVFKILGAISICHLVNDMLASLLPAIYPL
                     LKTSFNLDFTQIGLITLTYQTTASLLQPMIGFYTDKNPRPYSLPVGMGATLIGLILLS
                     IAHSFSALLVAAALVGTGSSVFHPESSRVARMASGGQHGLAQSLFQVGGNAGLSLGPL
                     LAAFIVMPRGQLSIAWFTLAALLGMILLTGVSGWAKKRRLTWKKFSAKHVDKPPNLST
                     SRITASIAILMALLFSKFVYLASLTSYYTFYLMNKFHLSVQSAQVHLFVFLGAVAAGT
                     LIGGPVGDRIGRKTVIWVSILGVLPFTLLLPYSNLLWTEILSVVIGLILASAFSVIVV
                     YAQELVPGKVGMISGLCFGFAFGMAGLGAAVLGWLADLTSINFVYLVCSYLPAMGLLA
                     AFLPDIEHPGMKAIHEEALAD"
     gene            complement(2677..3138)
                     /locus_tag="VG272_03880"
     CDS             complement(2677..3138)
                     /locus_tag="VG272_03880"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWF12851.1"
                     /translation="MISLTVNKRGVNVEVDPSTPLLWVLREHLGLTGTKYGCGVSQCG
                     ACTVHLDGQPVRSCSTPVSRAVGKEVTTIEGLSLNRNHPVQRAWLEIEVPQCGYCQSG
                     QIMSAAALLKTNPNPADEDINRAMSGNICRCGTYPRIRRAIHRAAEMSKTV"
     gene            complement(3207..5414)
                     /locus_tag="VG272_03885"
     CDS             complement(3207..5414)
                     /locus_tag="VG272_03885"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWF12852.1"
                     /translation="MTDFPLKRRDFLVGIGVSGLGLVFGLDLKSTSATNTVDAPTTGA
                     GVKMAWVRIAPDGAITIMAPATEMGQGTTTALAVIFAEELDADWNKVSVEFSPADDAI
                     YGNPLPFFFGMMVTAASTAVMGYYKNMRLYGAQARRVLLDAAATRLNAPVAELTTEPS
                     VVVHAKTGQQLSYGEIASFAKLPETLPTVSEKDLKDPSKFRLIGHEIPRVDVPIKTDG
                     SATYSIDVRVPNMVYATVVRAPVLGATPISVNDAEVSKMRDVIKVIQLPPDRVAIAAR
                     TYEAALAAERALKITWNKIAQSEFDSERALDEQSAIARDLSKAGVQVQNTGDVVAAMN
                     GAAKVYKAEYRTEYVYHAQLEPLNSVSWVKDGGRSVEVWAGSQTPTHLTRSVAQALGI
                     TPENVQLHRTYLGGGFGRRSAQDHDWAVDSALVSKELGMPVKVIWSRESDVRFGRFKP
                     MTSQYLQAAEDSSGELIAWHHRLVADEALAMTDPTRFEKGKEFPRISTTGIKTDYDVP
                     NILVEAVRNKLGVRMSAVRGVGSTVNQFAAESFVDEIAIARGDDPVEMRLGLLRKSPV
                     EQEVLRAVANMAAWKRGEKEGRGVSYVMNAGTFMATIARVTVDRKTGVILVPEIWIAA
                     DVGVPIMPRNLDAQLQSAVIHSLSNLLKERITFKNGAVQQSNYYDYQVLRMSEAPEVH
                     THIMPSMRTPVGIGDLGGIGVGPAVANAFFSATGRRLRQSPFLPDRVLAVLKT"
     gene            complement(5773..6345)
                     /locus_tag="VG272_03890"
     CDS             complement(5773..6345)
                     /locus_tag="VG272_03890"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWF12853.1"
                     /translation="MTSTLNLSEKFASARSYDRYVQTGTDEQRRRWTQVYDAAHLTEA
                     QKQLVAGFVREMKILVFSGIWCGDCVQQCPLIHRIAEANPAKIDLRFVERPKESELPT
                     ELRINAGSRVPVVIFLSEDNEWCATAGDRTINRYRAIARAKLGPLCPTGIVAPDKDEV
                     DATLSDWLNEVERVQLMLRLTPRLRQKYQD"
     gene            complement(6623..>9320)
                     /locus_tag="VG272_03895"
     CDS             complement(6623..>9320)
                     /locus_tag="VG272_03895"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HWF12854.1"
                     /translation="RNLVIFVVLAIVALWASSTAADFIIEYNWWKELGQVNTWVGMLW
                     YSIAPAAAGSVIAFFALWIAHARGLHFSGVLKRDHPIYSMLIPLGLAVVAIAFASSSI
                     DYWTVMRFFGSRGLAAPADAWKDQVFSRGLPFYLFDLPFYGEVLGFVFILAILCALVF
                     WATARGWQLAERFRYGALDRGDHRTLTIEPNSLLLPGASRAGFVRVLTLILLLGFAVW
                     VYLGNYELLLNSHAFMTGADFVDEKVTLPLRWLLIAVSLGALPLLWMRQYKKAVSIVA
                     GFFVLQIILPIIVHAVYVRPNEISIERPYIERHIDATSIAFGLNRNAQELPFVPSKQA
                     NVDPVQDATLLDNVRLWDLRAYNATITQIQALRPYYTFPDTDVDRYFPNGRIKQVLLS
                     PREIDVNQLSAEASQSWINPRFIYTHGFGAVMSEVNKITPDGLPVLLIENAPPEIKEP
                     GFQLTRPEIYYGEKTQDPVFVHTAREEFDYPSGDQNKYSTYQGTGGFPVGSLLLKTAA
                     AISEGEPNIVFTSYLKGESRMMIHRNVQERLRYLAGFLDWDQDPYLVITDDGRLVWMV
                     DGYTSSQLHPYSATIPVPGLPDGVNYMRNAVKATVDAYNGKISLYIFDPSDPIIQSYQ
                     HLFPKLFLPASDMPADLRRHTRYPEALFQAQAEAYRTFHMRDPQVFYNKEDIWDIAHN
                     LAGQSSQPENVQATYVVATLPGEKSPEFLLILPFTPRGKDNLIGWMAARCDGDHLGNL
                     IFYQLPKQQLIYGPMQIESRIDQDQNISKDLTLWNQQGSHVLRGNIISLPVTGGFLYV
                     ESIYIQANEARMPQLKKVVLAMGDRLVYRDNFDQALAELVGGGLPGKAAPGAVVTGQP
                     ASASSPGNDTAAIADRVRQMRDQAEQLVKELEQLEKDAGTKK"
BASE COUNT         2110 a         2452 c         2577 g         2181 t
ORIGIN      
        1 cacgctggat ctttttccgc gtttgaaaat ctgcgccgcg catggcggtg gctacttgcc
       61 ttcctactcg agcagaggcg accaatgcct gacggcattt cccaatcttt gcaagccttt
      121 gaaaaaactt ccgagcgaat atctaaagca actttatttt gattccgtac tttttagtcc
      181 agaagacatc agacacctgg tcgctgtagt aggtgcaagt caagttgtcg ttgggacaga
      241 ctatcccacg ctttggaatc gtactcccgt aggccgaatt ctgacggttc ccggattaag
      301 tgacgaagaa cgtatttcaa tttttagcgg cacggcggcg aagttgctca agatcaatct
      361 gtgaatttat ccgacagagg agatgcaatg aagcaaaaag ttgaggctcg ggaaaaccgt
      421 aatcgtcgca attttttgaa ggcagccgga agcgtggcag ccattgggct cgccgccgcg
      481 aacaattcgt ttgcgcagag cgaaaattcc aaaagctccg cgcatatgga gcgcatgaag
      541 atggcgccgc cggatcgagg catgtggatt acctggtacg atctgccaac cgaaggccgc
      601 gacgcttatt tttcctggct gcacgggaca tatcttccgg cactcttgaa gcggccaggc
      661 tatttgtggg cggcacacta cgcgtcgcag gatctggaag gcggcgatga aaatgcaaaa
      721 cgctatcatc atgtggacga tccgaaggtg ggaaagggtt ttcattacat gcttctgatt
      781 gccgcgaccg atgcgaatgc gtttggagat cccgtcccca gcgtcctgca tgcggtgctg
      841 cccgagaaag ataaaaaaat gctcgcgata cgaaccggcg agcgcataaa tatttttacg
      901 gagactgggc ggcgcgatgg cacggcaaac ggcgcgtata aagaaggaat gttgggcacg
      961 ccgtgcattc aagtgggaag ttttgattgc gcgctggagt tcgaagaaga gatgcatgcc
     1021 gggtatgtac tcgaacggtt gccgaaaatg tgcgcgacgc catcctgcgt tcgagtgcgc
     1081 aaactcaact ctgtggttgg atgggcgaag cacggtatta tttatgagtt ctcgtcgaag
     1141 gaaggtttcg accgcgacta cgcgccggct gtcgcgaaat ctcccctggg gttgaacggg
     1201 cattccgtgg ttccggagct ggtacacgcg ccgaacgggc cgaatagcgc gttgcgaatt
     1261 tggccgccag tgccgaaggc gggcgtcgcg taaccggagc cgctcgaagg ttttcatctc
     1321 ggagaattgc cgaatcttga tacaataatc gaaaggggtt cttccgtggt gcagtgtcct
     1381 aagaggtggg gaaaagaact tggcagcgac tcaaaccgaa tcattggtgg atgtggggca
     1441 tgaagttgtt ttcaagattt taggtgcgat cagcatctgc catcttgtga atgacatgct
     1501 cgcttcgtta ttgcctgcga tttatccatt attgaaaact tctttcaatt tagattttac
     1561 ccaaataggc ctgataactc ttacctacca gaccactgca tctttgctgc aacccatgat
     1621 tggcttttac acggacaaga atcccaggcc gtattcgctg ccagtgggaa tgggggcgac
     1681 gctcatcggg ctgattcttt tatcgatcgc gcactcattt tcggccttgc ttgttgcagc
     1741 agcgctcgtg gggacgggat cttcggtctt tcatccggaa tcatcccggg tggcgcgcat
     1801 ggcgtcgggc gggcagcatg ggttggcgca atcacttttc caggtaggcg gaaacgcggg
     1861 actttcgctc gggcccttgc tcgccgcctt catcgtaatg ccgagagggc aattgagtat
     1921 tgcgtggttc acgctggccg ctttgctggg gatgattctg ctaacgggtg taagcggttg
     1981 ggctaagaaa cgccgcttga cctggaaaaa attcagtgca aagcacgtgg ataaacctcc
     2041 gaatttgtcg acatcacgaa tcactgcatc cattgccatt ttgatggcgc tgttgttttc
     2101 caaattcgtt tatctggcaa gcctgacgag ctactacaca ttttatttga tgaacaaatt
     2161 tcacttgtcg gtgcaaagcg ctcaagttca tctctttgtt tttcttggcg ccgttgcggc
     2221 gggtacattg atcggcggac ctgttggcga ccgcattggc cgcaaaactg taatttgggt
     2281 ttcgatcctc ggcgttctgc cgtttacctt gctactgcca tactccaatt tattgtggac
     2341 tgaaattctc agcgtggtga ttgggctgat tctggcttct gcattttctg tgattgtggt
     2401 gtacgcacag gaactggttc cgggaaaagt gggaatgatt tcaggtctgt gctttggatt
     2461 cgcattcggc atggcggggc tgggcgccgc ggtgctcggg tggctggcgg atttaaccag
     2521 cattaatttt gtgtacctgg tttgttccta cctcccagcg atgggattgc ttgcggcttt
     2581 tcttccggat attgaacacc ctgggatgaa ggcaattcac gaagaggcgt tggcggacta
     2641 gaggttttca gtagcgcggg cgtcagcctg cactacttaa actgtcttgg acatttctgc
     2701 tgcgcggtga atggcgcggc gaattctagg ataagttccg cagcggcaaa tatttccgga
     2761 catggcacga ttaatatctt catctgcggg gttcggattc gttttcaaga gtgccgccgc
     2821 gctcatgatt tgtccggact ggcagtagcc gcactgaggc acttcgattt ccagccacgc
     2881 acgttgcacg ggatgattcc ggttcagcga gagaccttcg atcgttgtga cttcttttcc
     2941 aaccgcgcga gaaaccggag tgctgcaaga gcgcaccggt tgaccatcga gatgcacggt
     3001 acacgcgccg cactgcgaaa ctccgcaacc atattttgtg ccggtcaatc cgaggtgttc
     3061 gcgcaatacc cacagcaggg gagtggaggg atcgacttcc acatttacgc cgcgtttatt
     3121 cactgttaat gaaatcattt gcaaaagcct cctcggcatc aaatctgctc gtgaccatcc
     3181 gttttcatgc acacgttttc aattccttac gtttttaaga ctgccagcac gcgatccggc
     3241 aaaaatggcg actggcgaag tcggcgcccg gtagccgaaa agaaagcgtt tgccacggca
     3301 gggccgacgc cgattccacc aaggtcgcca atgccaaccg gagtgcgcat tgacggcatg
     3361 atatgcgtgt gcacttccgg cgcttccgac atgcgcagca cttggtagtc gtaataattc
     3421 gattgctgaa cggcgccatt tttaaatgtg atgcgttcct tgagcagatt gcttagcgag
     3481 tgaatgaccg cgctctgcaa ttgcgcgtcg agattgcgcg gcatgatcgg cacgccaaca
     3541 tccgcggcga tccagatctc cggcacgaga atgacgcccg tcttgcgatc gaccgtgacg
     3601 cgcgcgatgg tggccatgaa agttccagcg ttcataacgt aagaaacgcc gcggccctct
     3661 ttctcccctc tcttccaggc cgccatgttc gccactgcgc gaagcacttc ctgctcgacg
     3721 ggcgatttcc gcagtagccc gaggcgcatc tcgacaggat cgtctccgcg cgcaatcgca
     3781 atttcatcca caaagctttc cgcggcgaat tgatttaccg tcgagccgac tccgcgcacg
     3841 gccgacatcc gcacacccag cttgttgcgc acagcctcga ccagaatgtt ggggacgtcg
     3901 taatcggttt tgattcccgt ggttgaaatt ctggggaact ctttcccttt ttcaaagcgg
     3961 gtgggatccg tcatcgccag ggcttcgtcc gcgacgaggc gatgatgcca cgcgatcaac
     4021 tcgccactcg aatcttccgc tgcctgaaga tattgcgagg tcatcggctt gaaacggccg
     4081 aagcgcacgt cggattcgcg gctccagatc acttttaccg gcatgcccaa ttccttggac
     4141 acaagcgcgg aatctacagc ccagtcgtgg tcctgcgccg agcgtcggcc gaagccgcct
     4201 ccgagatacg tgcgatgcag ttgcacattt tcgggggtaa tgccgagcgc ttgggctacg
     4261 gagcgggtca gatgggtggg cgtttgcgat cccgcccaga cttccacgct cctgcctcca
     4321 tccttcaccc aggaaacgga attcaacggc tcgagttggg cgtggtagac gtactcggtg
     4381 cggtattcgg ccttgtaaac tttggctgct ccgttcatag ctgcaaccac gtcgcccgtg
     4441 ttttgcactt gcactccggc cttgctcaag tcgcgcgcga ttgccgattg ctcgtcgagc
     4501 gcacgctcgc tgtcaaattc gctttgagca attttgttcc aggtgatttt cagcgcacgt
     4561 tcggcggcaa gggccgcttc gtacgtacgc gccgcaatcg ccacgcgatc gggcggaagc
     4621 tgtatcactt tgatgacgtc gcgcattttg gatacttccg cgtcgttgac ggaaatcggc
     4681 gttgcgccaa gcacaggagc gcgcacaacg gttgcgtaaa ccatgttggg cactcgcacg
     4741 tcaatgctgt acgttgccga gccatctgtt ttgatgggaa catccacgcg cggaatttcg
     4801 tggccgatca ggcggaactt gcttgggtct tttaaatctt tttctgaaac cgtgggcaaa
     4861 gtttccggca attttgcgaa gctagcgatc tctccatagc tcaactgttg tcctgttttt
     4921 gcgtggacaa ccacgctggg ttccgttgtg agttccgcca ccggcgcatt gaggcgggtc
     4981 gcggctgcat caagtaacac gcgtcgcgcc tgcgctccat agaggcgcat gttcttgtaa
     5041 tagcccatca ccgccgtgct cgccgcggta accatcatgc cgaaaaagaa aggcagggga
     5101 tttccgtaaa tcgcgtcgtc cgcaggacta aattcgacgg acactttgtt ccagtccgca
     5161 tcaagttctt cggcaaagat taccgccagc gccgtcgtcg tgccctgtcc catctccgtc
     5221 gccggagcca tgatggtgat tgctccatcg ggagcgatac gcacccacgc cattttcacg
     5281 cccgcgccgg tagtgggcgc atcgacagtg ttagttgcgg acgtggattt aagatcgaga
     5341 ccaaacacaa ggcccagacc gctcacgcca ataccaacga gaaagtcgcg acgcttcagt
     5401 ggaaaatcag tcatgttggg agcacctccg gacgcgcgga aatcatgtgc aatcgaatat
     5461 acgctgcacg caaatggtgt caaggcaggc ggaatgaaac aatcgcggcg aacgatcgaa
     5521 tgcataatta aagtaatctg tgtacgaagc gattttaatt cgcgtcagaa cgtcccacag
     5581 aaatacgttg gcgttttccg aagtcccgga gggacgtaac agaaagcaca cgcgtcgaga
     5641 agaaagccct agaggggcga cacagaggtt atcggcaaca ccgtgtgtca ccccgtcggg
     5701 gctttacatt aggtgctaaa gatgcttgct tgattttcta caagcccggg gatttccgat
     5761 tttgaaaaag gttcaatcct gatatttctg ccgcaggcgc ggcgtgaggc gcaacatcaa
     5821 ttgcacgcgt tctacctcgt tcagccaatc ggacaatgtc gcgtcgacct catccttgtc
     5881 gggagcgacg atacccgttg ggcagagcgg gccaagcttg gcgcgggcga tggcgcggta
     5941 acggttgatg gtccggtccc cggctgtggc gcaccattcg ttgtcttccg acaaaaatat
     6001 tacaacgggg actcggctgc ctgcgttgat gcgtaattcg gtgggcagtt cgctttcctt
     6061 ggggcgctcg acaaaacgca agtcgatttt tgcgggattg gcctcggcga ttcgatgaat
     6121 cagagggcat tgctgaacgc aatcaccgca ccagatgccg ctgaaaacta gaattttcat
     6181 ttcgcgcacg aagcccgcga cgagttgctt ctgagcttca gtcaagtgtg cggcatcgta
     6241 gacttgggtc caccggcgac gttgttcatc cgttccggtt tgaacgtaac ggtcgtagga
     6301 gcgagccgaa gcgaattttt cggagaggtt tagagtgctt gtcatgtttg acggaatatc
     6361 tcctgtattt ctccattcat tccgttgtgt ggccacaaaa gaacacgctc caaaagcaag
     6421 aaaaggaaac tttctgtcat tccgagcgaa gcgaggaatc tctcttcggt ttaatttaag
     6481 taagcaaggg aaagagagat tcctcgctcc gctcggaatg acagagtaga actacttttt
     6541 acgcaaagtg ccaaccctga ctgtagttcg gcgttttagg gccagaatat aaaagtcaaa
     6601 agaaaacagg ctgaagcctg ttctactttt tagttcctgc gtctttttcc aattgctcga
     6661 gttctttcac cagttgttcc gcctggtcgc gcatttggcg cacacggtcg gctatcgctg
     6721 ctgtgtcatt tcccggcgac gatgctgacg cgggctgccc agtcaccacg gctccgggcg
     6781 cggctttccc tggtaagccg cctcctacca attcagcgag tgcctgatcg aaattgtcgc
     6841 gataaacgag gcgatcgccc atggcgagga caactttttt gagctgaggc atgcgcgctt
     6901 cattcgcttg aatgtaaatg gactcgacgt acagaaagcc gccggtcacc gggagcgaaa
     6961 taatatttcc gcgcaagacg tgcgagcctt gctggttcca caacgtcagg tccttggaaa
     7021 tattctgatc ctgatcgatg cgcgactcga tttgcatggg tccgtatatt aattgctgct
     7081 ttggcagttg atagaaaatc aggtttccca ggtgatcccc atcgcagcgc gcggccatcc
     7141 agccgatcaa attatccttg ccgcgtgggg taaatggaag aatcagcaga aactccggcg
     7201 atttttctcc cggcaaagtg gctacaacgt aggttgcctg cacattttca ggctgcgaag
     7261 attggccggc cagattgtgc gcaatatccc agatgtcttc cttgttatag aaaacctgag
     7321 gatcgcgcat gtgaaacgtg cggtaggctt ctgcctgcgc ctgaaataag gcttcgggat
     7381 agcgcgtgtg ccggcgcagg tccgcgggca tatccgaagc aggaaggaat aattttggaa
     7441 aaagatgctg gtacgactga atgatgggat cactcggatc gaatatgtag agcgaaattt
     7501 ttccgttgta ggcatcaacg gtggccttca ccgcgttgcg catgtaattc acgccatcgg
     7561 gcaatccggg caccggaatc gtcgcggaat atggatgcag ttgggaagac gtatagccgt
     7621 ccaccatcca aaccaatcgt ccgtcatcgg taatcacgag atacggatcc tgatcccagt
     7681 cgagaaaacc cgcaaggtaa cgcaatcgtt cctgcacatt gcggtgaatc atcatgcggc
     7741 tttcgccctt gaggtagctg gtgaaaacga tgttgggttc gccttcagag atcgccgccg
     7801 cggttttcaa aagcagcgac ccaaccggga atcctcccgt accttgatag gtggaatatt
     7861 tattctgatc gccagagggg taatcaaatt cttcgcgcgc tgtgtgaacg aaaaccgggt
     7921 cctgcgtttt ttctccgtaa taaatttcag gcctggtcag ttgaaagccc ggctctttaa
     7981 tttccggcgg agcattttca atcagcagga cgggaaggcc gtccggggtg attttattaa
     8041 cctcggacat aactgcgcca aacccatgcg tataaatgaa acgcggatta atccaactct
     8101 ggctcgcttc cgcggataac tggttgacgt cgatttcgcg cggagacagc aacacctgtt
     8161 taatacggcc gttggggaag taccgatcga cgtccgtatc ggggaaagtg taataaggcc
     8221 gtagcgcctg aatttgcgtg atcgttgcat tgtaggcgcg caaatcccac aggcgaacat
     8281 tgtccaggag cgtggcgtcc tgcacgggat ccacgtttgc ttgcttggac gggacgaatg
     8341 gaagttcctg cgcattgcga ttcaggccga aggcgatgga cgtcgcatcg atgtgccgct
     8401 cgatatatgg ccgctcaatg gaaatttcat ttggccgcac ataaacggca tgaacgataa
     8461 tcggcagaat tatttgcaat acaaaaaagc cggcaacaat cgaaacggct ttcttgtatt
     8521 gccgcatcca cagaagtgga agcgcaccga gcgacacggc aatgagcaac cagcgcaagg
     8581 gaagcgtaac tttttcgtcg acgaaatccg cgcctgtcat gaacgcgtgg gaattgagca
     8641 gcagttcgta attcccaaga taaacccaca cagcaaaccc aagcagcagg ataagcgtaa
     8701 ggacacgcac aaaacctgcg cgactcgctc ccggcagcag cagggaattc ggctcgatgg
     8761 tcagagtcct gtggtcgccc ctatcgagcg cgccgtagcg gaagcgttcc gccaattgcc
     8821 aaccccgcgc cgtggcccaa aataccagtg cgcaaagtat agccagaatg aaaacgaagc
     8881 cgagcacttc cccataaaag ggcaaatcga agagataaaa aggcaatccg cgtgaaaaga
     8941 cctggtcctt ccacgcgtcg gcgggtgccg ccaagccacg cgaaccgaaa aagcgcatca
     9001 cagtccagta gtcaatcgaa ctcgacgcga acgcgatcgc gaccacggcc agtccaagag
     9061 gaatcagcat ggaatagatg gggtgatcgc gcttgagaac ccctgaaaaa tgtaatccgc
     9121 gcgcgtgagc gatccataat gcaaagaacg caatgaccga ccctgcggca gcgggcgcaa
     9181 tgctgtacca aagcatcccc acccacgtat ttacctggcc aagttccttc caccagttgt
     9241 actcgataat aaaatcggcc gccgtggagc tagcccatag agcaacaatt gccaggacta
     9301 cgaatatgac caggttacgg
//
LOCUS       DASZMB010000082         3561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_369192, whole genome shotgun sequence.
ACCESSION   DASZMB010000082 DASZMB010000000
VERSION     DASZMB010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369192"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VG272_03900"
     CDS             <1..182
                     /locus_tag="VG272_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12855.1"
                     /translation="YAFVYTPVTADASGHTITYTLTADPTNAGVTGQRHFYSDQTCVI
                     RSNISTAAGPSDVPL"
     gene            298..975
                     /locus_tag="VG272_03905"
     CDS             298..975
                     /locus_tag="VG272_03905"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HWF12856.1"
                     /translation="MSALLAIGASGQTLPPAASDQAKPLTHLLEEHYRRPRTLQAVFL
                     ERFNEGKKQERVESGTVYFRRPGQMRWDYDSPEKKLFLVDGKTTWFYVPYDRTVTKSP
                     VKESSDWRTPLSLLTGKADLSRMCSKIDLVSEPGVPSGHAILRCVPKGEKSASSQETY
                     TEVLLEVDTSSGELARIEIHQPGGIDLEYRFGNWQTDIPLTADLFKFQIPVGVAIVNG
                     ASMEGPQ"
     gene            1188..2399
                     /locus_tag="VG272_03910"
     CDS             1188..2399
                     /locus_tag="VG272_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWF12857.1"
                     /translation="MAEFVCKVADGSGKVFLHVEPAQSMSEARQKLSDRGLFVYNVRP
                     RAGVVGQLFGRKRQKSVDGNDFLVFNQQFNTLIKAGIPILRALDLLSERAAAEKLRPV
                     LGEVRRQVREGTNLSEALEAQGVFPKVYTVSVLAGEKSGNLSGVLEYYIAYQKVTTGF
                     RKKLISTLIYPIILVCSAIGIVTYLVTYVIPKFAGLYNDLNINLPAATRLLLAITVTY
                     RPYLLASLIVAALLIAAVFAWSQTSQGGLTFERIRMRLPLIGDTITKFQFAQFCRTLS
                     TLLAGGTPLVQAMETAGGAIRSRLVSGAVSSGAQLVREGQSLHDSLSSTGLVPDLALE
                     MIEVGEASGSLSAMLASVAEFYEDDVNTHLANLVALIEPAILVFMAVLIAFILVALYL
                     PMFSLSAAGQG"
     gene            2412..>3561
                     /locus_tag="VG272_03915"
     CDS             2412..>3561
                     /locus_tag="VG272_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HWF12858.1"
                     /translation="MSTIDQSLTTASDPQSEREKSRRLAERYRCGFVDLFEQRIDPEL
                     FRSIPAELMFRFNFVPMAAQDGALIIAVADPSQVLLSDELPLLLGKKLILKVATPTQI
                     SDLLKRTEQSQRVLDQATEAFTLTVVEEDDETDSNISADSLTQQDSAVSPVVRLVDTV
                     IFTALERRASDIHIEARDIEVAVKYRIDGVLVHAMAPISKDWLSTIISRIKVMSELDI
                     AERRVPQDGRFRVRYKGRFVDFRVSIMPSSHGEDAVLRVLDKETLSEKFQNLSLDVVG
                     FSPDEMRKFRRYIREPYGMVLVTGPTGSGKTTTLYAAINEIKTDEDKIITIEDPVEYQ
                     VRGITQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDNETAQIAIQS"
BASE COUNT          782 a          961 c         1005 g          813 t
ORIGIN      
        1 gctatgcatt cgtctacacg cctgtcaccg cggatgcctc gggccacacg ataacctata
       61 cgttgacagc cgaccccaca aatgccgggg ttactgggca gcggcacttt tactccgatc
      121 agacttgcgt aattcgatcg aacatctcga cagctgccgg cccgtccgat gttccgctgt
      181 agctcacgta accggtcctg ctaaaacggt cgccgaattc ctcgttgttc ggttcttcct
      241 gctatactcc attttgtgaa tctactcgtt aaggcgaacg ttttcgcgtt tatttttttg
      301 tctgcgcttt tggcgattgg tgcttcaggg cagactcttc cgccggcagc ctctgaccag
      361 gcaaaacccc tcactcactt gttggaagag cattaccgcc gaccgcgcac tttgcaggct
      421 gtttttctgg agcgattcaa cgaagggaag aagcaggagc gcgtggaatc cggcacggtt
      481 tatttccggc gtcccgggca gatgcggtgg gactacgatt ccccggaaaa gaaacttttc
      541 ctcgtggatg gaaaaaccac gtggttttac gttccatacg accgcaccgt tacaaaatcc
      601 ccggtaaagg aaagctcaga ctggcggact ccgctctcgt tgttgacggg gaaggcggat
      661 ctttcgagga tgtgcagcaa gatcgacctt gtgtcggagc ccggggttcc aagtggccac
      721 gcgatcctgc gctgcgtccc gaagggtgag aaaagcgctt ccagccagga aacctatacc
      781 gaagttttat tggaagtgga cacgagctcc ggcgagctgg ctcgcatcga aattcatcag
      841 ccaggcggca tcgacctgga atatcgattc ggaaattggc aaacggatat tccgttaacc
      901 gcggatcttt ttaagttcca aatccccgta ggagttgcta tcgtaaatgg cgcgtctatg
      961 gaaggcccgc agtaaaaaac gttggttgaa aagttgaaca gggaatccac acccagccgt
     1021 agttgaccat cctttaccgg attttcaaat gaaagtcaca gattagaaat atgttgccca
     1081 ttcggtgtga ctacagtgga aaaggctcat gctaccggtt gccacccagt acacgctgcc
     1141 gtgagcgcgc ctttctgcat ctatagcgac tgaggtacac tcaggatatg gctgaatttg
     1201 tctgcaaagt ggctgatggc agcggaaaag tttttctgca tgtggagccg gcacaatcga
     1261 tgtccgaggc tcggcagaag ctttccgacc gtggtctatt tgtgtataac gtgcggccgc
     1321 gcgcgggggt ggtgggccaa ctgtttgggc gcaagcgcca gaaatcagtt gatggaaatg
     1381 attttctggt ctttaaccag cagttcaata cgctgattaa agcgggcatc ccaatcctca
     1441 gagccttgga tttgctttcc gaacgggcgg cggcggaaaa attacgtccc gtgctgggcg
     1501 aagtccggcg ccaggtgcga gagggaacga acctatccga agcgctcgag gcgcaaggag
     1561 tattcccgaa ggtctacacc gtctctgtgc tggccggaga aaaaagcggc aacctttccg
     1621 gtgtgctcga gtactacatc gcttaccaga aggtgacgac cggctttcgc aagaaactga
     1681 tttccacgct catttacccg atcattttgg tttgctcggc tattggcatc gtcacctacc
     1741 tcgtcacgta tgtgattcca aaattcgcgg ggctgtacaa cgatttgaat attaatttgc
     1801 cggcggccac gcggctcctg ctggcaatta ccgttaccta caggccgtac ttgctggcct
     1861 cgctgatcgt cgcggccctc ctgattgcag ccgtatttgc gtggtcgcaa acttcgcaag
     1921 gcggattgac gttcgaacgc attcgaatgc ggctgcccct gatcggcgat acgattacga
     1981 aatttcagtt cgcgcaattc tgccgcacgc tttccacgct gctcgcggga ggcactccgc
     2041 tcgtgcaagc catggaaacc gcgggcggag ccattcgcag ccgccttgtt tccggagcgg
     2101 tttcaagcgg tgcgcaactc gtgcgagagg ggcagtcgct gcatgacagc ctttcgtcca
     2161 ccgggctcgt gccggatctg gcattggaaa tgatcgaagt gggcgaggcc agcggatccc
     2221 tgtcggccat gctcgccagc gttgcggaat tttacgaaga cgatgtgaac acgcacctcg
     2281 cgaatctggt ggccctgatc gaacccgcca ttctggtttt catggcggtg ttgattgcat
     2341 ttatcctggt ggcgctgtac ctgccgatgt tttcgctgtc ggccgctggt cagggatagg
     2401 gatagaattt tatgagcaca atagatcaat cacttaccac agcatccgat ccgcaaagcg
     2461 agcgggagaa atctcgccgc ctggcggagc gctatcgctg cggatttgtc gacttgttcg
     2521 aacaaagaat cgatccggag ctgtttagga gcattcccgc ggagttgatg ttccggttca
     2581 actttgtgcc gatggcggcg caggacggcg ccctgatcat cgcagtggcc gatcctagtc
     2641 aggttctgct cagcgatgag cttccgctct tgctcggaaa aaaactaatt ctcaaggttg
     2701 ccacgccgac gcaaatcagc gacctcctga agcgcaccga gcagtcgcag cgcgtccttg
     2761 accaggcgac cgaagcgttt accttgacgg tggtcgaaga ggacgacgaa accgattcga
     2821 acatttccgc cgacagtctc acgcagcagg attccgcagt cagtccggta gtgcgcctcg
     2881 tggacacggt aatcttcact gcgctcgaac ggcgcgcgtc ggacattcat attgaggcgc
     2941 gggacatcga agtggcggtg aagtaccgca ttgacggcgt gctggtgcac gcgatggcgc
     3001 ccatttcgaa ggactggctt tccacaatta tttcgcgcat caaggtgatg agcgaactgg
     3061 acatcgccga gcgccgcgtg ccgcaggacg gacgtttccg tgtgcgctac aaaggccgct
     3121 tcgtcgattt tcgagtttcg attatgccgt cgagccacgg agaagatgcg gtgcttcgcg
     3181 tgctggacaa ggaaacgctt tccgaaaaat ttcaaaacct cagtttggat gtggttggtt
     3241 tttcgccgga tgaaatgcgc aagttccgcc gctacattcg cgagccgtac ggcatggtgc
     3301 tggtcacggg tccaacgggc tccggaaaaa cgacgacgct gtacgccgcc atcaacgaaa
     3361 tcaaaacgga tgaagacaag atcattacga ttgaagatcc ggtggaatat caggttcgcg
     3421 gtattacgca gatccctgta aatgaaaaaa aggggctgac gtttgcgcgc ggattgcgct
     3481 ccatcctgcg gcatgacccg gacaaaatca tggtcggcga aatccgcgac aatgaaaccg
     3541 cgcaaatcgc cattcaatcc g
//
LOCUS       DASZMB010000083         4351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_369359, whole genome shotgun sequence.
ACCESSION   DASZMB010000083 DASZMB010000000
VERSION     DASZMB010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369359"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VG272_03920"
     CDS             <1..667
                     /locus_tag="VG272_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12859.1"
                     /translation="RNQLGGLSRDRQGRQSGQGQQGGGQGQGQSGQAQGQGQGQGGGQ
                     GGQQMGQRGSGPGGAFGARMGDGTYGGGYRNGNYGGGGNNGTIYGNFDTGNNTPRGGP
                     PVQPDNSPVPTQRVYDDTLRDLNQLRQAVRDDPESAKEVQQLIQEMERLDPARFPGNP
                     ALVEQLHSQVLADVDKLELQLRRQLDDKDAGQVRSGDTKTVPQGYEESVAEYYRRLSK
                     TSK"
     gene            complement(676..2262)
                     /locus_tag="VG272_03925"
     CDS             complement(676..2262)
                     /locus_tag="VG272_03925"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWF12860.1"
                     /translation="MSHLRVELFSDMVKKGFFLPAKLSVVFVLGFAFAAGAAAQSEWF
                     TPDPSIVSKFHEEEAQHSHVMEVLGYLTDVYGPRLTNSPNIREAADYAVKTLTSYGLS
                     NVHEETWGPFGRGWSSEWFEANEIAPRDFSLIAYSKAWTPGTNGVVTADAIFAKIEKE
                     EDFAFYRGKLRGLFVLTAPMRAVDPLFDPLAHRLTDQELAGMVQARAPQPPDKAARDR
                     QRKLLEFNAKLIKFLTDEGAAAWLEPTAHDGGTVTVMAGGSRDPKDPLVLPRIAVDIE
                     NYGRIFRTVEKNIPVKLRINIVNKFYDDNLDSFNIVAEIPGTDKADEVVMLGAHFDSW
                     AGGDGATDNAAGSAVMMEAMRILKASNLKMRRTVRLALWTGEEEGLLGSRAYVKEHFA
                     DRASMTLKPDHAKLSGYFNVDNGTGKIRGIYLQDNEALRPIFEKWMEPFKPEGMTTLS
                     SNGTNGTDHVSFDEVGLPGFQFIQDPIEYETRTHHTNMDVYERLQPADLKQMAVIVAS
                     FVYMTANADQLLPRKILPKQ"
     gene            complement(2378..2605)
                     /locus_tag="VG272_03930"
     CDS             complement(2378..2605)
                     /locus_tag="VG272_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12861.1"
                     /translation="MSVISEKVIAISKHYFGPATESFLDRQCTGHLNIHIDSLAASHL
                     KDLAKWVESSGALLMDAAKAAEVAAKIAKVA"
     gene            complement(2678..4063)
                     /locus_tag="VG272_03935"
     CDS             complement(2678..4063)
                     /locus_tag="VG272_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12862.1"
                     /translation="MSVFRTSVKKTGSILVLGAAGLICIVLLQGTSAIAQNKNAAVQT
                     TNMISAQRTEAFIAAHNAVEPPSTNSVAGAPAAAPLLAATPSHSGQAGATTAAPATPQ
                     APAPAEPPPAEEESHEHMMEIPHGPVLKFRGFFDLDFDDGPVAQNLNFPLGVPAKTSF
                     RAGEFDLFISSQIAEKLSFLTELVFSSGPENVFGVDLERFQLTYRYSKYFEISAGRYH
                     TSIGYYNTAFHHGTWFSTATGRPFAFFFEDSGGPLPIHELGVTTTGLVPHTGKINLHW
                     IAEVGNGSSEVGNPLYGDGVENFASDRNHKDVNVAFYIRPEWVEGLQIGASFLTGDLV
                     TSGGTVKVNQTVTSAYAVLIDSRWEFLNEFVLLHHQAAVGGQTFNSPMGYTQLAYHIG
                     KARPYFRWQEVNIPSADPVTTFKGRYEGPSGGIRYDVFTYAALKFQYNRVYFRNAAAE
                     NGVELQLAFTF"
     gene            complement(4119..>4351)
                     /locus_tag="VG272_03940"
     CDS             complement(4119..>4351)
                     /locus_tag="VG272_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HWF12863.1"
                     /translation="TVSYKLARAILVPVDHLKEVAENVSMGNLDMSVRRYSDDEIGDL
                     ADSFSRMVTAVKFFRMEAQIHNSEQAAHQVKH"
BASE COUNT          977 a         1229 c         1165 g          980 t
ORIGIN      
        1 gcgcaatcag ctaggagggt tgtcacgcga ccgccaagga cgccagtcgg ggcaagggca
       61 acagggcggc gggcagggac aagggcagag tggccaagcg cagggacaag gtcaaggcca
      121 gggtggaggc caaggcggcc agcaaatggg gcagcgaggc agcggtccgg gcggcgcatt
      181 tggcgcacga atgggcgacg ggacatacgg tggcggctat cgcaacggaa attacggcgg
      241 tggaggcaat aacggcacga tttacggaaa ttttgacacg ggaaacaata cgccgcgcgg
      301 cggtccgccg gtgcagcctg acaattctcc tgtacctacg cagcgcgtct acgacgatac
      361 gctgcgcgat ctgaatcaac tgcggcaggc cgtgcgcgac gatcccgaat ccgcgaaaga
      421 agtgcaacaa ctcattcagg aaatggagcg tctcgatccg gcgcgcttcc ccggcaatcc
      481 cgcactcgtc gagcagcttc attcacaggt gctcgcagac gtcgacaaac ttgaacttca
      541 actgcgccgt cagctcgacg acaaagatgc cgggcaggtg cgcagcggcg acacaaaaac
      601 cgttccgcaa ggttacgaag aatccgtcgc cgagtattat cgccgcctga gcaaaacgag
      661 caaatagcct taaggctact gtttcggcaa tattttgcgc ggcaaaagct ggtcggcatt
      721 cgcagtcatg taaacaaagg aagcgacaat cacagccatc tgtttcaaat ctgctggctg
      781 aagccgttcg taaacatcca tgttggtatg gtgggtgcgc gtttcgtact caatcggatc
      841 ctgaataaac tgaaatcctg gcagccccac ttcgtcgaaa gacacgtggt ccgtgccgtt
      901 cgttccgttg gacgaaagcg tggtcatgcc ctccggcttg aacggttcca tccacttttc
      961 aaaaatgggg cgcagcgctt cattatcctg tagataaatt ccgcgaattt ttccggtacc
     1021 gttatccaca ttgaaatacc cggaaagctt cgcgtggtcc ggcttaagcg tcatcgacgc
     1081 ccgatccgca aaatgttctt tcacatacgc gcgggagccg agcaatccct cttcttcgcc
     1141 agtccacagc gcaagccgca cggtgcgccg cattttcagg ttgctcgctt tcaagatgcg
     1201 catcgcctcc atcataacag cggatccagc cgcattgtcg gtcgcgccgt ccccgccggc
     1261 ccacgaatcg aaatgcgcgc cgagcatcac cacttcatcc gctttgtccg tccccggaat
     1321 ttcggcgacg atgttgaatg agtccagatt gtcatcgtaa aacttgttca caatattgat
     1381 tcgcagcttc accggtatat ttttttccac cgtgcggaag atgcgcccgt agttttcaat
     1441 gtccacggcg atgcgcggca ggaccagagg atccttggga tcgcgcgagc ctcccgccat
     1501 caccgtaacc gtgccgccat cgtgcgcggt gggttcgagc cacgcggcgg cgccttcgtc
     1561 cgttagaaat ttaatgagct tggcgttaaa ttccagcaac ttgcgctgac ggtcgcgcgc
     1621 agccttgtca gggggttgtg gcgcccgcgc ctgtaccatg cctgctaatt cctgatcggt
     1681 caggcgatgc gccagcggat cgaataacgg gtcgaccgcc cgcatcggcg cggtcagaac
     1741 gaacagaccc ctgagcttcc ctcgatagaa tgcaaaatcc tcttcctttt ctattttggc
     1801 gaatatcgca tcggccgtga cgaccccgtt tgttccaggc gtccacgcct tggaatatgc
     1861 gatgagcgaa aaatcgcgcg gagcgatttc attcgcttca aaccattcgc tcgaccagcc
     1921 tcgcccgaac ggcccccacg tttcttcgtg cacgttcgac aagccatatg atgtcagcgt
     1981 tttcactgca tagtccgcag cctcgcgaat gttcggcgaa ttcgtcaggc gtggtccgta
     2041 cacatcggtg aggtagccga gcacttccat gacgtgcgaa tgctgcgcct cttcttcgtg
     2101 aaatttgctg acgattgagg gatcgggtgt gaaccattcg ctctgcgcgg cggcgcccgc
     2161 ggcaaaggca aaccccaaca cgaacacaac gctcaacttg gctggaagga aaaagccttt
     2221 tttcaccata tctgaaaata attctactcg aagatggctc aattacgatg tgcagccgct
     2281 atgcgagttc caccgcggcc acaataggta caaacttcgt gaagaaagac tgaggattcg
     2341 tgtccgtcag acgctgatgc tttccaggtc caacgaacta tgccaccttg gcaatttttg
     2401 cggccacttc agcggctttt gccgcatcca ttaagagagc acccgaactt tccacccact
     2461 tggccagatc tttcaggtgc gacgctgcca acgagtcgat gtgaatgttc agatgccccg
     2521 tgcattgcct gtccagaaaa gattctgttg cgggcccgaa atagtgcttg ctgatcgcga
     2581 ttactttttc tgaaattaca ctcatcgatc tccccattcc gccgattcaa ctcatatctg
     2641 cgacaggtgc acagtttctg agatttattc cggttcctta aaacgtgaac gccagctgaa
     2701 gttccacacc gttttcagcc gccgcgttgc ggaaataaac gcggttgtat tgaaacttga
     2761 gggcagcata ggtgaacacg tcgtaccgaa tgccgccgga cggtccttcg taacgccctt
     2821 taaacgtcgt gacaggatcg gcggaaggaa tatttacttc ctgccaccga aaatagggcc
     2881 gcgcctttcc gatgtgatac gcaagctgcg tgtagcccat cggcgaatta aaagtttgtc
     2941 cgccaacggc ggcctggtga tgcagcagga caaattcatt caggaattcc cacctcgaat
     3001 caatcaagac tgcatacgcg ctcgtcacgg tttgattcac ttttactgtt ccgcccgatg
     3061 taacgaggtc accagtaaga aaagatgcgc caatttgaag accttccacc cattccggcc
     3121 gaatatagaa agcaacgttc acgtccttgt ggttgcggtc tgatgcaaag ttttcaacgc
     3181 catcgccgta caatgggttg ccaacttcag agcttccatt gcccacttcc gcgatccagt
     3241 gcaggttgat cttgccggtg tgcggcacga gccctgtcgt agttactccc agttcatgaa
     3301 tgggtaatgg gccgccgcta tcctcaaaga aaaacgcgaa gggtctgcct gtggcggtgg
     3361 aaaaccacgt cccgtggtga aacgctgtgt tgtaataacc gatggacgtg tgatatcggc
     3421 ctgcgctgat ctcaaaatat ttgctgtagc ggtaggtaag ttgaaaccgc tcgagatcca
     3481 ctccgaatac attttctggt cccgatgaaa aaaccagctc cgtcaaaaaa ctcagctttt
     3541 cagcgatctg cgaactaatg aacaaatcga attcgccggc tcggaacgat gtttttgcag
     3601 gaactcccag tggaaaattt aaattttgcg ccacaggtcc atcgtcgaag tccagatcga
     3661 agaatccgcg aaatttaagc accggcccgt gaggaatttc catcatgtgc tcgtgtgact
     3721 cctcctctgc gggtggcggc tcggctggtg cgggtgcttg cggcgttgca ggggccgcag
     3781 tggtcgcgcc tgcttgtcct gagtgagaag gagtcgccgc caagagcgga gccgcagcag
     3841 gtgcgccagc tacggagttt gtagaaggcg gttcaaccgc attgtgtgcc gcaatgaacg
     3901 cctcggtccg ctgcgcggag atcatattcg ttgtttgtac cgccgcattt ttgttctgcg
     3961 caattgctga ggttccctgc agcagcacga tgcagatcaa gccagcggcc ccgaggacga
     4021 gtatgctccc cgtttttttg acacttgtgc gaaagacact cattcgtttc tccccctcga
     4081 acttcttgcg tgagattttt ctgttgaatg gcccccgctc agtgcttcac ttggtgagcg
     4141 gcttgttcgg agttgtgaat ctgcgcttcc atccggaaaa acttcacggc cgtgaccatg
     4201 cgcgaaaagg aatcagcaag gtccccgatt tcgtcatccg aataccggcg gacggacata
     4261 tccagatttc ccatgctgac gttctctgca acttctttca gatggtcgac tggaacaaga
     4321 atggcccgcg cgagcttgta gctcacggtg a
//
LOCUS       DASZMB010000084         4365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3385829, whole genome shotgun sequence.
ACCESSION   DASZMB010000084 DASZMB010000000
VERSION     DASZMB010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3385829"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="VG272_03945"
     CDS             complement(<1..540)
                     /locus_tag="VG272_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HWF12864.1"
                     /translation="MEATIHVFLSYLRVEKSLATNTILAYGRDLKRFCEFLRKRQKNK
                     MEDVTREDVVDFLSSLYKEKMDSRSVARYLVSLRGLYKFAMMENMVTTDPTENLESPK
                     VRSSLPTYLRVEEINKLLEAPNLSTPIGLRDRAMLEILYSTGLRVSELLNLRMTDVDM
                     RVGCVRCIGKGDKERLVPIG"
     gene            complement(547..2166)
                     /gene="murJ"
                     /locus_tag="VG272_03950"
     CDS             complement(547..2166)
                     /gene="murJ"
                     /locus_tag="VG272_03950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HWF12865.1"
                     /translation="MTETVTHKRQILKSASIISIITVVSRVLGYVRDQRLTLLLGTTG
                     LADSFVLAYRIPNILRRLVGEGSMTASFIPVFTDYMRNKSREETWEFANRLFWTFCVI
                     LSALTVLGVIFSPLFVRFFSMFGKNQLQFEAIYLNRLMFPYILFIGMAAMAMAILNCF
                     HIFGPPAATPILLNISFIVFSIAAVWKHFSSPAAALAVGVLVGGIFQFFWQVPQLAKR
                     GMNFKFAVSFSDPGVRRVVRLMVPGFIGMGIAQINLLVDTIFANAKIMPEGSLVSLYV
                     ADRVTELVLGGYAIAVATAIMPMMSHQAAAGDHEGMKKTFLFALRIVSFITIPATVGL
                     VILREPIVQVLFQHGRFVAESTRLTARALLYYSLGLPAFAAVKLIVPAFYSTQDTRTP
                     VRVAVLAMLANVLLNVVFLFYFFPKLKNGGPALASALAAYFNVFALFVIFRLRFGRLG
                     TRDVAASLGKIAASAGVMGFVCWGALKYSQFDSIEHFIPRLLVFIALIGAATLTYLGL
                     AWAFRCDEISEVYGIAFHRAAAEPGKPGILG"
     gene            2293..2775
                     /locus_tag="VG272_03955"
     CDS             2293..2775
                     /locus_tag="VG272_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12866.1"
                     /translation="MKVRTPSSTHKKVVVRFFGRGDLRGYLNPGRLGEADGVELLTPD
                     GEQRNFELKDVRSIYFVRDFAENFEPERKVFLSRPKLDGLWVRLKFRDDDTIEGVVPN
                     DLLSLLDRGVQITPPDLHGTTVKIFIPRTAMTELVVLGVVGIARRKPPAPPQPSLFND
                     "
     gene            complement(2828..3022)
                     /locus_tag="VG272_03960"
     CDS             complement(2828..3022)
                     /locus_tag="VG272_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12867.1"
                     /translation="MGTTLYGLCPGCNNRFYTAGAPKFCGKCGTPVITNCPQCNIPVA
                     HYKEHLADFCEACGTGLRKV"
     gene            3351..>4365
                     /gene="lpxD"
                     /locus_tag="VG272_03965"
     CDS             3351..>4365
                     /gene="lpxD"
                     /locus_tag="VG272_03965"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HWF12868.1"
                     /translation="MKLGELAAKLDCKLDGGDETLEVRGVAGIERAQAGEITFLANPK
                     YARELATTLASAALVEESAAIVREAGLPSLAALRSKNPYFDFARAIELFSTPPEYQSE
                     IHPTAVIAKTAKIGEGAHVGPHCFVDEGVVIGRNAVLHSLVTIYRDAQIGDDFLAHSQ
                     TVVRERCRIGNRVILQNGAVIGADGFGFAKNSHGRWHKIMQNAPVVIEDDVEIQANSC
                     IDRASVGETRIRRGVKIDDLVVVGHACDVGEDTLLCGQVGLAGSTRVGRNCILAGQVG
                     SAGHLDIAEGAIVTAQSGIHQDIPTAGLWSGSPLMDNKTYRKSFAAYKRLPELQNKLR
                     KLAG"
BASE COUNT         1082 a         1221 c         1179 g          883 t
ORIGIN      
        1 accgatgggg acgaggcgct ccttgtcccc cttgccaata caccggacgc agccgacgcg
       61 catatcgacg tccgtcatac gcaagttcag caactcggaa acgcgcaatc ccgtcgaata
      121 caaaatttcc agcatcgccc ggtccctcag cccaatcgga gtggaaaggt tcggagcttc
      181 cagcagctta ttgatttctt ccaccctcag gtacgtgggc aaactgctgc gaactttagg
      241 cgactcgagg ttttcggtag ggtccgtggt gaccatgttt tccatcattg cgaatttgta
      301 gagtccgcgc agcgaaacca gatagcgcgc cacggagcgg ctatccatct tttccttata
      361 aagactgctc agaaaatcga ccacatcttc acgcgtgacg tcttccatct tatttttctg
      421 ccgcttgcgc agaaattcgc aaaagcgttt cagatcgcgt ccataggcga gaatcgtatt
      481 cgtcgcgaga gatttctcga cgcgcagata gctcagaaat acgtgaatcg tcgcttccaa
      541 ttctctttat cctagaattc ccggtttccc aggttccgca gcggcccggt gaaacgcgat
      601 gccatacact tcgctgattt cgtcgcagcg gaacgcccat gccagtccca gataagtcag
      661 cgtcgctgcg ccgatcaagg cgataaacac gagcaaccga gggatgaaat gttcaatgct
      721 gtcaaactgc gaatatttta acgcgcccca gcaaacgaaa cccatcacgc cggcgctcgc
      781 cgcaatcttt ccgagcgagg ccgccacatc gcgggtgccg agccggccaa atcgcaaacg
      841 gaaaatcaca aataacgcaa atacgttgaa ataagcggcg agggcgctgg ccagcgcagg
      901 gccgccattt ttcaatttcg gaaaaaagta aaacaggaat acgacgttca gcaaaacgtt
      961 cgcgagcatc gcgaggaccg ccacgcgcac cggggtccgc gtatcctggg tcgaataaaa
     1021 cgccggtacg atcaacttaa ctgccgcgaa cgctggaagc ccgagtgaat aatacagtaa
     1081 cgcccgggcc gtcaaccgcg tagactccgc tacgaagcgg ccatgctgga acagcacctg
     1141 cacgatcggc tcacgtagga tgaccagacc cacagtcgca ggtatcgtta taaacgaaac
     1201 gatacgcaat gcaaaaagaa acgtcttttt catgccctca tggtcacccg ccgccgcctg
     1261 gtgcgacatc atcggcataa tggctgtcgc cacggcaatc gcgtagccgc ccaagacgag
     1321 ttccgtcacc cggtccgcga catacaggga gacaagactg ccttccggca tgatcttggc
     1381 gttcgcaaaa atagtatcca ccagcagatt gatctgcgcg atgcccatcc cgataaatcc
     1441 gggaaccatc aggcgcacca cgcggcgcac acccggatcg ctaaacgaca cagcaaattt
     1501 gaaattcatt ccgcgcttcg cgagctgcgg gacctgccag aaaaactgaa agatacctcc
     1561 aaccaacacg cccacggcga gagccgccgc cggactggaa aaatgcttcc ataccgcagc
     1621 tatggagaac acaataaagg agatattcag caaaatcgga gtggctgccg gaggcccgaa
     1681 aatatgaaag caattcagaa tggccatggc catggccgcc atcccgatga acaggatgta
     1741 aggaaacatc aagcgattca gatagatagc ttcgaactgc agctgattct ttccaaacat
     1801 agagaaaaat cgcacaaata gaggtgaaaa tatcactcca agaaccgtta gggccgacaa
     1861 tatcacgcaa aacgtccaaa acagacgatt cgcgaattcc caagtctctt cccggctctt
     1921 gtttcgcatg tagtcggtga aaacggggat aaaagaggcg gtcatggacc cttcgcccac
     1981 caatcggcga agaatattgg ggatccggta cgccaacacg aaggaatcgg ccaagcccgt
     2041 ggtacccaaa agaagcgtta gacgctgatc acggacgtaa ccaaggactc ggctgacaac
     2101 cgttatgatg ctaatgatgg aagcagattt gaggatctgc cgcttatggg tgacagtttc
     2161 ggtcaaaaga acgaggcccc cagtcacagc ctctatctaa acctttgata aacatacact
     2221 tgacagccaa agagcgctta acataacata cttgcgctcg actcgcaagg cccgcccttc
     2281 gagtccgctc ctgtgaaagt acgaactcca tcgtctacgc ataagaaagt cgtcgtgcga
     2341 ttcttcggtc gcggggactt gcggggatac ctaaatcccg gcaggctggg cgaggcggac
     2401 ggcgtcgagc tgctcactcc ggatggcgaa caacgcaatt tcgaactgaa agatgtccgc
     2461 agcatttatt tcgtgcgcga tttcgctgaa aatttcgagc ccgaacgcaa agtatttctt
     2521 tcccgtccca agctggatgg gctgtgggtg cggctgaagt tcagggatga cgatacgatt
     2581 gagggcgtgg tgcccaacga cttgctttcc ctgctcgatc gcggcgtgca gatcactccc
     2641 ccggacttgc acggcacaac tgtaaaaatc tttattccgc ggacggccat gaccgaattg
     2701 gtggttttgg gcgttgtggg tatcgcacgg cgcaaaccgc cggcgccacc acaacccagt
     2761 ctctttaacg attagcaaat ctcccaccca gaatggatct ggaataaccg atggcaacgc
     2821 gtggcgttca gactttgcgc aggccggtgc cgcaagcttc gcagaaatcg gcgaggtgtt
     2881 ccttgtaatg cgcgaccgga atattgcact gcggacaatt ggtgatgacc ggagtgccgc
     2941 acttcccaca aaatttcggc gcccccgccg tatagaagcg gttgttgcag cccgggcaca
     3001 atccgtatag cgtcgttccc atctatcctc ctcagcttag tgcctgggcc cgcgcagcgc
     3061 gatttgccga ccttttagca atgataccag aggtcgcaat ctccttttgc gtcgcgaaat
     3121 gcgatctggt cggatatata actcgaagtt ctggcatggc gcagcttcga tctatgaacc
     3181 cgaacgactg gttgacgaag tcgccctctt cgtcccgaaa agcacgggac gaacagtcgt
     3241 ttatggcggg tcgaatttct acgtcttcgt ggaatgatgt acagtgagga atcaatcggg
     3301 cgtggatttt tctgtatgtt ccgcgaatcg ggatttgagg agaatgagac atgaagctcg
     3361 gcgaactagc cgcaaaactg gattgcaaac tggacggcgg cgacgaaact cttgaagtgc
     3421 gcggcgtcgc gggaatcgag cgtgcgcaag ctggtgaaat tacgttcctg gcgaatccaa
     3481 aatacgcgcg cgaactggcc accacactcg cgtcggcggc acttgtggag gaatccgcgg
     3541 ccatcgtgcg tgaagccgga ttaccttcgc tcgcggcgct gcgctccaaa aatccttact
     3601 tcgatttcgc gcgggccatt gaattatttt ccacgcctcc agagtatcag tcagagattc
     3661 atcctactgc ggtgatcgcg aagactgcga aaatcggtga aggcgcgcac gtcggcccgc
     3721 actgtttcgt cgatgagggc gtggtgatcg gccgcaacgc cgtgctacac agccttgtga
     3781 cgatctaccg ggatgcgcag atcggcgacg actttctggc gcactcgcag actgtggtaa
     3841 gagagcgctg ccggatcggg aatcgagtga tcttacaaaa cggcgcagtg attggcgcag
     3901 acggtttcgg tttcgccaaa aattctcacg gccgctggca caagatcatg cagaacgcgc
     3961 ccgtggtgat tgaagatgac gtcgagattc aggccaactc gtgcatcgat cgcgcttcgg
     4021 tgggagaaac gcgaatccgg cgcggagtaa aaattgacga cctcgtcgtc gttggccatg
     4081 cgtgcgacgt cggcgaggat acgcttctct gcggacaagt cggtctggcc ggttctacac
     4141 gcgtgggacg taattgcatt ctggccgggc aggtagggtc agcgggacac ctggacatcg
     4201 cggagggcgc cattgttacg gcgcagagcg gcatacatca ggacattccc accgcaggtc
     4261 tgtggtcggg ctctcctctg atggacaaca aaacgtaccg gaaatcgttt gcggcctata
     4321 agcgccttcc ggaattacag aataaactgc gtaagttagc cggcg
//
LOCUS       DASZMB010000085        16054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1468701, whole genome shotgun sequence.
ACCESSION   DASZMB010000085 DASZMB010000000
VERSION     DASZMB010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 16054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16054
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468701"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="VG272_03970"
     CDS             complement(<1..1213)
                     /locus_tag="VG272_03970"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HWF12869.1"
                     /translation="MKRPVKSYRIFSTVNVLFLLIAVFSGKAYGDTKPKFVYVANSGT
                     SSTAIGGSVSGYAVDATTGALSPLPGSPFAAGSGPSSVATDPSNKFLYVANHLSNNVS
                     AFTVDAKTGALTAIAGSPFAAGSAPVALAVDPLGNLVYVVNQTSNNLSVYTLNSQTGA
                     LTAISGSPFQTGISPNSVAVDPLGRFVYVTNFVFPQTIGPLVSDIFAYSVDRNADTLH
                     SVLGSPFTVNAPVYSTVDPSGKFLYVSIDNGTNIFGLGGYGIDGSTGALTAITGARYG
                     LGQPSAAAFDPSGKFFYALDDRPPAGGYDIAAFSFDSSTGLLSALPTSPFAAGAYPLA
                     ISIDPSGKFVYAPNFSGNNISAYTIDATSGNLTAVPGSPFLTGVSPVSVAILSSSSTA
                     FKKFEATAEIDE"
     gene            complement(1469..2038)
                     /locus_tag="VG272_03975"
     CDS             complement(1469..2038)
                     /locus_tag="VG272_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWF12870.1"
                     /translation="MITTERLILRLWKDEDFQPYAEINADPRVREFFPTILTREKSDA
                     EVRHIQSAYARDGFTMFAAELIATGEFIGFIGMVTMTFAVPGLAQPSIEIGWRLAHNH
                     WGKGFATEGALGVIRYGFETVKLKEIVAITVPANVRSRHVMEKIGMKHFPDLDFDHPR
                     IPEGHPLRKHVLYRVKNDVTPEVVIGIFR"
     gene            complement(2293..3072)
                     /locus_tag="VG272_03980"
     CDS             complement(2293..3072)
                     /locus_tag="VG272_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWF12871.1"
                     /translation="MSQEVLYQLNDSVGRITFNRPEARNAFTFRMYERLVEICKEASA
                     NPAVKVLVLSGVDETAFAAGTDISEFKKWSSAQDALDYEVRINSILDGIERIPIPTIA
                     AIAGACTGGGGTIAACCDIRIAAANARFGVPIARTLGNCLSMANYVRLVGLLGQARVK
                     DILFTGRLVDAAEAHTIGLVSEVLPDYATLQTRADELARIVASRAPLTLRVTKEALRR
                     IKEKMTPEEDASMILECYMSHDFQEGMDAFLNKRKPKWTGT"
     gene            complement(3069..4319)
                     /locus_tag="VG272_03985"
     CDS             complement(3069..4319)
                     /locus_tag="VG272_03985"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWF12872.1"
                     /translation="MENANKQKSKGPLEGLKVVDLSHIMAGPTCSMFLADMGADVIKV
                     EKIPGGDDSRRMVPPKIAEESAAFLIMNRNKRGIALNLKTEEGREVLTRLLKNADVVI
                     ENYRRGTMEKMGFGYETLHALNPKLIFCAISGFGRTGPYADRGGFDLVAQGMTGLMSI
                     TGERQGGPPMKAGAPVTDITAGILACVGILAALHARESTGQGQMVDTSLFEAGITHTY
                     WHSAICFATGKAPGPMGSAHPLNGPYQAFPASDGWITVGAANQENWLRLVDALEAPEL
                     RDDSRFVNNAQRMSNLSALTDALTPLFKRRTVADWLRRLEEVGVPAGPVLDIAQMHAD
                     PQALAREMIVETTHPVAGRVKAIGLPIKFSDTPGSVRRAAPVFGQHTREVLREQNYSE
                     EEIDHMLEQGAVQTPESTKKGATR"
     gene            4543..5745
                     /locus_tag="VG272_03990"
     CDS             4543..5745
                     /locus_tag="VG272_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12873.1"
                     /translation="MKRILFPASLCLTLFATVLLVSSPAQAQTTYNANVGAETRDQAV
                     QADAFLPNELWLLEGDSIKWTFVPQNEVHTVTLLTPGQIRPSAPPPAGPPFVVQGVNC
                     GPAASYDGSACVSTATGLSGGATFTVKFPKAGNYKFVCLVHTDMTGTVHVLVNNATNG
                     ALIHGQRFYDDQAHDEAEAILSDNDRRGDDRDEWNHRKNDENSVVAGTGEIVGTGGGM
                     QYRAVVRFLPGVIRIHAGESIVWTNLDPTEPHTVTFGTEPAGFVPTLFSLPPAVLLPA
                     GPPIPNPPAPCTSTLTTNCTDSSTGTIIAEINCTTSPAATAGTTPCKAAFNPQTDSVS
                     PPPPPFAQFSFLNSGFLQAAPPDRTGTAQVPPGRTRIRITFPNKGTYYYHCALHDVDG
                     MNGTVVVE"
     gene            5783..6427
                     /locus_tag="VG272_03995"
     CDS             5783..6427
                     /locus_tag="VG272_03995"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HWF12874.1"
                     /translation="MRAFLVLAFLFGTLQSGAAQASKNIPGSASGGGAEPKYRGGLVS
                     PPLPKPKFTLTDTSAAPFDFATKTQGYVTLLFFGYTHCPDMCPLQMSTLAQAYRKLPL
                     VIADRFKVVFVTTDPARDTPQVLRSWLDHFDKRFIGLTGSQAAIDAAQVSADMAPAKK
                     SAKRSDGNYEVGHALFVFAYTKDNLAHVVYPGGVREEDWSHDLPFLPSENWTSR"
     gene            6708..7202
                     /locus_tag="VG272_04000"
     CDS             6708..7202
                     /locus_tag="VG272_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12875.1"
                     /translation="MNNRIMRGFFWGMVATILMTTMHILIWAVTGRLTVMALATRTMP
                     AMIIAKMFGTGLSTPVHLLLAMAIHLGYGGLWGAILFALTPRVTIWKGLAMGAFLYVG
                     AQVFLLPLLGRGMFGAGSPDRMFGIWFSIATHSTFGASLGWFGSLKEYSAGTGQKPAP
                     APGD"
     gene            7362..9050
                     /locus_tag="VG272_04005"
     CDS             7362..9050
                     /locus_tag="VG272_04005"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HWF12876.1"
                     /translation="MNKTRRIRRTISIAALALILDAPAFAAPQETAPAPITDQDLLGG
                     LKNPARWLTFSGDYNGQRHSPLKQLTPRNVAGLVPQWIFQTDIPGMAGRGIENTPLVV
                     DGILYITGNNNTAWALDGRTGRPIWSYRRRLPNNFIASVCCGPVNRGFGILGDKLYMG
                     TLDAHLIALDRKTGAVIWDVAVGDLKKANAITAAPLVIKNKVIIGVAGGDFSSRGYIE
                     AFDAQTGERAWHFDTIPMPGEPGSDSWPNANVAARGGGAVWVTGSYDPSLNLVYYGTG
                     NPNPDYYGDDREGDNLYTCSLVALDLDTGKLRWHFQFTPHDVHDWDSAHVPVQAELKI
                     GGQPHKVIMVANRNGFFYTLDRETGKLLVAKPFIDGSNWAKEIGKDGRPIVLDNNGTA
                     DKCLPDNHGGTNFQPPTFDPELGLFFVTAHETCATWQGVKPTEPIEMGVRVPSGGRVL
                     VENHNQFSALRAIDPTTGQRKWEHKYRDYPSTVALDLTGGVMSTASGLVFTGDNEGYF
                     YAFEGATGKELWKFQTGAPVWGSASVTYMLDDRQWVVTTAGLTFTAFALPATSR"
     gene            complement(9101..10708)
                     /locus_tag="VG272_04010"
     CDS             complement(9101..10708)
                     /locus_tag="VG272_04010"
                     /inference="COORDINATES: protein motif:HMM:NF004780.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWF12877.1"
                     /translation="MADTQVLIVGAGPVGLTLALDLGLRGVRCTLIERNTTSIQLPKM
                     ERCNARTMEIYRRLGISERVRDAGLPRNAPMDVFLALSMAGPSLAHLPCPSVTEAKAA
                     IAAHNDGGLLEPYQLISQYTLEPLLRSILEALPSVTVRFGCELVSFTQDLDSVTGLVQ
                     SNNSMDETIRAAYLVGCDGGSSTVRKQLGIRLEGDGNIRKLRQALFRADDLYERIPMG
                     KGRHYHIAEGPLFPFLILQDSTRHWTLHAAASSDAEMAEIFRKSLGMPIEFEMLSVNE
                     WTQHLLCAERYGEGRVFIAGDAAHLVIPTGGLGMNTGVGDAIDLSWKLAAMLARWGGS
                     QLLASYEVERRPIGLRNVRASRAAMTGRQGWRAAYHPNIKDNTPEGAATRRKIATLFD
                     TEQRKVTEISGIESGYRYANSPIVWQEPGDGPDPDNLVYLPTSWPGARLPHVWLSDGI
                     ALHDRLGPGYTLLRLGGTKADTSRLERLFLEACAPLEVLDISNQRAREIYEYDLLLVR
                     PDLHIVWRGNQFSDADAAKIAAIATGQ"
     gene            complement(10822..11994)
                     /locus_tag="VG272_04015"
     CDS             complement(10822..11994)
                     /locus_tag="VG272_04015"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWF12878.1"
                     /translation="MNDFLKRYAKLALGQPFSPAMLNILVTSVCDMRCTHCFFTEQLD
                     DAPRKKLQMTTREIEKISETLGGNLGVLVLAGGEPFTRKDLPEIARAFYTNNNLESLY
                     ITSNGQIQKRIFPDIFRILKECPSLNVTMALGIDGLKPQHDTIRQKTGSWDIAIDTAR
                     QLQSMKREYPRLDVQTCTCFMNSNQDTIFEWYDFLKFELKPDKVNFNYIRPPVADQKE
                     LQIDSARYAALAAKIDEDSRRSTIKNHYAGSNGFFKAALDVYMHGLIAKTRETQQAQL
                     TCYAGTAGGVIYDEGTVSSCENLEPVGNLREHDWNFQQLWFSDSMQARRKKAADGCFC
                     THESNCYYPSLPMNPKHLIQIKLLEREMKNSWNKNVGAATAYLDDRVRANAAARSR"
     gene            complement(12160..13566)
                     /locus_tag="VG272_04020"
     CDS             complement(12160..13566)
                     /locus_tag="VG272_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF12879.1"
                     /translation="MNSSNGPAGAELQKGAQTQLGATDARSLLARMEQVPFSGWHRNA
                     RLIMGSATFFDAFNALSLAFALPTLIRLWHISPKQSGLLISASYVGQLAGALAFSALA
                     EKVGRIRGTTAAIAIMSIMSFGCALAGNFPALLACRLAQGIGVGGEMPVAATYINELS
                     RAHGRGRFFLLYEMIFPLGLMATGQLGAWFVPAFGWQSIFLFGGVPGLVIAYLVARLP
                     ESPRWLISKGRLQEAEKIVEQIEASTPRRIPAAENSRAVSVPNLMQRGKWTELLSTFY
                     RGRTLIVWLLWASAFFIANGLNNWMASLYNTVYHLSLRQSLRAASMTNIAQVAVLLIC
                     AFSIDRVGRRNWTVGAFILGGGLLAYLGMTGAQNSMSVMIFGTLGYGLIGSINAILYL
                     YTPEIYPTRMRAIGTGLATSWLRIASALGPALVGFMVGADGIHSVFLMFAGVSVIGAI
                     AATRMVETSDRRLEEIAP"
     gene            complement(13872..15032)
                     /locus_tag="VG272_04025"
     CDS             complement(13872..15032)
                     /locus_tag="VG272_04025"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HWF12880.1"
                     /translation="MGAKKLSIAIVGAGIGGMATAATLRQIGMDVHVYEQAPRFLRIG
                     AGIQMMPNSMKVLRKIGVEEKVIATSFKPYSHLNRKWDTGEVIRELPMPETLFGAPYL
                     CMHRADLHRALAAVVPEEIVHLDKKLVGLEQSAGQITLTFADGSRAHADAVVGADGVH
                     SVVREIMVGADEPIHKGRIAYRAIFPAALLKGMDIGASRTKWWGVDRHIVIYYTNADC
                     SELYFVTSIPEPAEWLTRESWSAKGDVKELRQAYEGFHSDVRAVLEACPDCHKWAILE
                     REPLDRWSEGCVVLLGDAAHPMTPYMAQGGATAIEDAAVLARCLAETSGEDIPGAFQR
                     FEAHRKPRTSRIQAISSANTWMKGGDEDTSWLYGYDAWNVPLTPSEPFVKTT"
     gene            complement(15184..>16054)
                     /locus_tag="VG272_04030"
     CDS             complement(15184..>16054)
                     /locus_tag="VG272_04030"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HWF12881.1"
                     /translation="RASSTSRVTVVPSGKALGAEIQGVDLRAVSADNFESIHQAWLDH
                     SVLLFRNQKLTDDDLIAFSKHFGDLDLAPIQETGRRFVEGHPEIYVVSNVMENGVPIG
                     SLGAGEATWHTDMSYLPDPPKASILYALEVPPLGGNTYFSSMYRAYEILPDALKKRVE
                     GKLLKHDGTYNSGGYVRQGVTAVDDPVQSPGTFHPLVCTHAETKRRGLYLGRRRNAYI
                     QGLSLRDSEALLDELWSYMDREEIAWHNEWRVGDLVFWDNRCTMHRRDPFDASSRRVM
                     HRTQIKGQASPAA"
BASE COUNT         3583 a         4362 c         4395 g         3714 t
ORIGIN      
        1 cctcgtcgat ttcagcggtg gcctcaaatt tcttgaaggc cgtgctggag gagctgagga
       61 tggcaacgga taccggtgat actcctgtca aaaatggcga accggggacg gcggttaagt
      121 ttccagacgt ggcatctatc gtatacgcgg aaatgttgtt gcctgagaaa tttggtgcgt
      181 agacgaattt gccggaaggg tcgatcgaaa ttgcaagcgg atacgcgccg gcagcgaaag
      241 gtgaagtcgg gagtgctgat aacaggcccg tggagctatc gaagctaaac gccgcaatgt
      301 catatcctcc agcgggaggg cggtcatcga gtgcatagaa aaacttacct gacgggtcga
      361 acgccgccgc gctgggttgt cccaacccgt accttgcacc ggtaattgcc gtcagcgcgc
      421 cggtgctccc gtctatgccg tagccaccaa gaccaaatat gttagttccg ttgtcaatag
      481 atacgtaaag gaacttgccg gatggatcta ctgtcgaata taccggggcg ttcacggtga
      541 atggtgatcc aaggaccgaa tgcaaggtgt ccgcgtttcg atcaacggaa tatgcaaata
      601 tatcggaaac aaggggccca atggtctggg gaaaaacaaa atttgttaca tagacgaacc
      661 ttccaagcgg gtccaccgcc acggagttcg gactgatccc tgtctggaaa ggcgaacccg
      721 aaatcgcggt caatgcgccg gtctgactat tcaacgtgta aactgaaaga ttgttggaag
      781 tttggttcac cacgtagaca aggttcccga gcggatcaac ggccaaagcg actggagctg
      841 atccggccgc aaacggcgaa cccgcaatcg cagtcaaagc ccccgttttc gcatctaccg
      901 taaaggctga gacattgttc gaaagatggt ttgccacata taaaaattta ttggacggat
      961 ccgtcgcaac ggaggaaggt cccgatcccg ccgcaaatgg cgagcccggg agtgggctca
     1021 gagcgcccgt tgtggcatcg accgcgtagc cggatacgct tccaccgatt gcagttgagg
     1081 atgtcccgga atttgccaca tagacgaatt tcggttttgt gtcaccataa gctttcccgg
     1141 aaaacactgc gatcagaagg aacaggacgt ttaccgtgga aaatattcta tacgatttga
     1201 ctgggcgctt catcgcactc tcctgattag aatgatcctg gctactcgga tgaactgggt
     1261 gtttaaaaaa gatctactct cagccttttc gattatttct gcccaagcaa agggcaaacg
     1321 atgtctggta ctggcaattg catgcccgtt ctaatttcat tgaaaataaa gaacttagat
     1381 gtacgtgttt actactctgg catttatgtt gcccataaag agaaatctac gtcacatgtg
     1441 aataaaccat tttaaaatca attgcgcact atcggaagat accaatcacg acctcggggg
     1501 tgacatcatt cttgactcga tacaggacat gttttcttaa ggggtggccc tccgggatgc
     1561 gcgggtgatc gaaatcgaga tctggaaaat gtttcattcc gattttttcc attacgtggc
     1621 gcgagcgaac attggcgggc accgtaatag ccacgatttc ttttagctta acagtttcaa
     1681 atccgtagcg aatgacgcct agggcgcctt cggttgcaaa tccttttccc caatggttat
     1741 gggcgaggcg ccacccaatt tctatggatg gttgagccaa accgggtacg gcaaaagtca
     1801 tcgttaccat tccaatgaaa cctataaatt caccagtcgc aattaattca gcggcaaaca
     1861 tggtaaatcc atctcttgca taagcggatt ggatatgtcg aacttcggcg tcgcttttct
     1921 cacgtgtaag aattgtcgga aagaattcgc gcactctcgg atcggcgttt atctccgcgt
     1981 agggctgaaa atcttcgtcc ttccaaaggc gaagaattaa tcgttccgta gtaatcatga
     2041 tcgttccccc aataataatt ccaagtcccg aagggacgaa acacaccgta gcccacagtt
     2101 ttaactgtgg gaaatctttg gctcggtgat agaaccgcgt aggggtgaca cacatggtcg
     2161 agtgagtgtc gcccctactg ggcttaattt catgcctgga caagttccaa gcgttgaaac
     2221 gctgggctac gatgtgacgt ccctttgggg actctgccgg gactctgctt tccagttctg
     2281 gcgtaataac ttctacgttc ccgtccattt tggcttgcgt ttgttcagga acgcgtccat
     2341 gccttcctga aaatcgtggc tcatgtagca ctccagaatc atgctggcat cttcttcggg
     2401 agtcattttt tctttgatgc ggcgcagggc ctctttcgtg acacgcaggg tgagcggcgc
     2461 gcgcgaggcc acgattcggg cgagttcatc ggcgcgagtt tgcaacgttg cgtaatcggg
     2521 aagcacttcg ctcacgagtc cgattgtgtg agcctcggcg gcatccacga gccgaccggt
     2581 gaagaggatg tccttgacgc gcgcctggcc cagcaggccg accagccgga cgtagtttgc
     2641 catcgacaaa caattgccga gcgtgcgggc tatgggcacg ccgaagcgcg cattggccgc
     2701 ggcaatgcga atatcacagc atgcggcgat ggtgccgcca ccgccggtgc aggctcctgc
     2761 aatggctgcg atcgtgggga tgggaattcg ctcaatgcca tccagaatgc tgttaatccg
     2821 aacttcgtaa tcgagcgcat cctgcgcgga ggaccatttc ttgaattcgg aaatatccgt
     2881 gcccgccgca aatgcggttt catccacgcc actaagcact aaaaccttaa cggccggatt
     2941 ggcggacgcc tctttgcaga tttcgacgag gcgctcgtac atacgaaacg tgaaagcgtt
     3001 tcgtgcctcc gggcgattga aagtgatgcg gcctacgcta tcgttaagct gatataaaac
     3061 ttcctggctc atcgtgtcgc gcctttcttc gtcgattccg gtgtttgaac agcgccttgc
     3121 tcgagcatgt gatcaatttc ttcttcggaa taattttgtt cccgcaacac ttcgcgcgta
     3181 tgttgcccga aaacaggcgc tgccctgcgc acgcttcccg gagtatccga aaatttaatg
     3241 ggcagcccga tcgctttcac acggccggct acggggtgcg tggtctcgac aatcatttcc
     3301 cgggcgagcg cctggggatc cgcgtgcatc tgggcgatgt ccagcacagg tccggcgggg
     3361 acgcccactt cttccaggcg tcgcagccaa tcggccacgg tgcgtcgttt aaatagcggc
     3421 gtcagtgcat ccgtgagcgc ggaaagattg ctcatgcgct gcgcgttatt cacaaagcgc
     3481 gaatcatcgc gcaactccgg tgcttcaagc gcgtccacga gccgcagcca attttcctga
     3541 ttcgccgcac cgaccgtgat ccatccgtcg gacgcgggga acgcctgata cgggccattc
     3601 aacggatgcg cggaacccat cgggccgggc gctttccctg tcgcaaagca gattgccgaa
     3661 tgccagtagg tgtgcgtaat tcccgcttca aacagcgatg tgtccaccat ctgcccttgc
     3721 ccggtggatt cgcgcgcatg cagcgcggcc aaaatcccta cgcacgcgag aatgccggca
     3781 gtgatatccg tgaccggcgc gcccgctttc atcggcggac cgccctggcg ctcgcctgtg
     3841 atgctcatga gtccggtcat gccctgtgcg accagatcga atccccctcg atcggcgtag
     3901 ggtccggtgc ggccgaatcc ggagattgcg cagaaaatta atttgggatt cagcgcatga
     3961 agcgtttcat atccaaagcc cattttttcc attgtgccgc ggcgataatt ttcgatcacc
     4021 acatccgcat ttttcagcaa acgagtcagg acttcgcgtc cttcctctgt tttcaagttg
     4081 agcgcaatgc cccgcttgtt gcggttcata atcagaaatg ccgcggattc ctctgcaatc
     4141 ttgggcggaa ccatgcgccg cgagtcatcc ccacccggaa ttttttccac tttgatgacg
     4201 tccgctccca tatcggcgag gaacatggag catgtcgggc ctgccataat gtgcgaaaga
     4261 tccacgacct tcaatccttc gagcggtcct ttgctttttt gtttgttcgc gttctccatc
     4321 catcgtcctt tctcaattcc cgatgctgag ctgtttcacg gaaaaacagt actgataatt
     4381 ctagtacccg cgcagaaaaa attgggcgca ttccgatttt caacttgaca ggatgcagtg
     4441 cgccgaatta agaatccttg tcgagaaaaa ttccatctgt tgaagataaa ctatcccgct
     4501 tccgtggaaa gacaattctt gaaaattttc tcagggggtt gaatgaaacg catacttttc
     4561 ccagcaagtt tgtgtttaac gttgttcgcg actgtcttgt tggtttcgtc gccagcacag
     4621 gcgcaaacaa cttacaatgc caacgttgga gcagaaacca gggaccaggc tgtgcaggca
     4681 gatgcctttc ttcccaatga gctttggctt ttggagggag acagcatcaa gtggacgttc
     4741 gttccccaga atgaagtcca tacggttaca cttttgacgc ccggacaaat ccgcccctcg
     4801 gcgccacccc cagcaggtcc gccattcgtt gtgcaaggtg tgaattgcgg ccccgcagcc
     4861 agctacgacg gcagcgcatg cgtatcgacc gccacaggtc ttagcggcgg cgcaacgttc
     4921 accgtgaaat ttccgaaggc gggcaattac aagtttgttt gcctggtcca cacggatatg
     4981 acaggaacgg ttcacgttct ggtcaataat gccaccaacg gagccctgat ccatggccaa
     5041 cggttttacg atgaccaagc gcatgacgaa gcggaagcga ttctctcgga caacgatcgc
     5101 cgcggagatg accgtgatga atggaatcat cggaagaatg acgagaattc tgttgtggca
     5161 ggaaccggag aaatcgtagg caccggaggc ggtatgcaat atcgcgccgt tgtgcgcttc
     5221 ctgcccggcg tgattcgcat ccatgcggga gaatccatcg tgtggacgaa tttggatccg
     5281 accgagccac atacggtcac gtttggaacg gagcctgcgg gatttgttcc cacgctcttc
     5341 tcgctgccgc ctgcggtgct tctgcctgcc gggccgccaa tacccaatcc gccagcaccc
     5401 tgcacttcaa cgctgacaac aaattgcacg gactcgagca ctggcacgat catcgcggaa
     5461 attaattgca cgacgtctcc tgctgcgacc gcgggcacga caccctgcaa ggccgcgttt
     5521 aatccgcaaa cagactcggt ttctccccca ccgccgccat tcgcacaatt tagtttcctc
     5581 aactccgggt tccttcaggc cgcaccgccg gatcgaaccg ggacagcgca agtccctccg
     5641 ggaagaacgc gaatccgaat tacgttcccg aacaagggca cttactatta ccattgcgct
     5701 cttcatgacg tagacggcat gaatggcacc gtggtagtgg agtagtttgc ggacaggtca
     5761 aataatgaag ttccgcttat tcatgagagc gtttttggtt ttggcatttc tgtttggcac
     5821 gctgcaaagc ggggcggctc aggcttcgaa aaacattcct gggtccgctt ctggcggtgg
     5881 cgctgaacct aaatatcggg gcgggctcgt ttccccgccc ctcccgaaac caaaatttac
     5941 gctcaccgat acttcggccg ctccattcga ttttgcaacg aaaacgcagg gatacgtcac
     6001 gcttcttttc tttggataca cgcattgccc cgatatgtgc ccgctgcaga tgtccacgct
     6061 tgctcaagcc tacagaaagt tgccgctcgt gatcgcggat cgatttaagg tcgtattcgt
     6121 gacgaccgat ccggcgcggg atacgccgca agtgctgcgc tcatggctcg accattttga
     6181 taaacgattt attggactca cgggaagcca ggcggcgatc gatgccgccc aggtatccgc
     6241 ggacatggct ccggcgaaaa agtcggcgaa gcgctccgac ggaaattacg aagtaggcca
     6301 cgcgctattt gtttttgcat acaccaaaga caatctggcg catgtggttt atcccggggg
     6361 tgtacgggaa gaggattggt cccacgatct gccttttctt ccttcggaaa actggacgag
     6421 tcgctgaaat tttcaaattt cacttcggat tcctggaaac ctcccgctca acatcttttt
     6481 caaaaatcgg tttttcgagc ggtagtgtgg gaagttgggt attgaacatt gggagtttct
     6541 gctggccggc aagaccagct tggaaatctt agtgcaaagc agcgtggagc gagtgtagga
     6601 tgctgccatc ggagagaaag tcccggtaga atcgattcaa attcatacct ctcgaatgac
     6661 gacgggtgcg ttctgccgct agtgttgaac cttcacgagg aaaactcatg aacaaccgaa
     6721 taatgcgcgg ttttttctgg ggcatggtgg caacgattct gatgaccacg atgcatatct
     6781 tgatttgggc cgttaccggg cgacttaccg ttatggctct ggccactcga acgatgcccg
     6841 ccatgattat tgcaaaaatg tttgggacag gtttgtccac accggtgcat ttgcttctgg
     6901 cgatggcgat ccatttgggt tacggaggcc tctggggagc aattctgttt gctcttacgc
     6961 cgcgtgtcac catttggaag ggcctggcta tgggtgcatt tctatacgtt ggtgctcaag
     7021 tcttcttgct cccgcttctt ggacgcggga tgttcggtgc ggggtctcct gaccggatgt
     7081 ttggaatctg gttttccatc gctacacact ccacttttgg ggcgtcactt ggatggttcg
     7141 gaagcttgaa ggaatatagc gctgggacgg gacagaagcc cgcccccgcg ccgggtgatt
     7201 gaatttttaa tattgttttc ttgattcgaa tttccgctct gttccggtaa aagcgaccgt
     7261 ttagttgctt tttttagaag gttaatgggt tgtgtcgaat caaacggaag ggtacgatgc
     7321 cggaatcggc tactaaaaaa tagaattctg ggaaaggtat tatgaacaag accagaagga
     7381 ttcggcgaac gatttcgatt gcggcgctcg ccctcatact tgacgcgccc gccttcgcag
     7441 cgccgcagga aaccgcgccc gcgccgatta cagatcaaga cttgctcggc ggcttgaaaa
     7501 atccggcgcg ctggctgact ttttcgggcg attacaacgg gcagcggcac agtcctctga
     7561 aacagttaac gcctcgaaat gttgctgggc tcgttccgca atggattttc caaacggaca
     7621 ttccagggat ggctgggcgc gggattgaaa acacgccgct cgtggtggac ggcattttat
     7681 atataaccgg aaataacaac acagcgtggg cgctcgatgg acgaactggc cgcccgatct
     7741 ggagctatcg gcgcaggctg ccgaataatt ttatcgcttc tgtttgctgc ggaccggtga
     7801 atcgtgggtt cggaattctg ggcgacaagc tgtacatggg tacgctggac gcacatctca
     7861 tcgcgctcga tcgaaaaacg ggagccgtga tttgggatgt tgcggtgggc gatctgaaaa
     7921 aggcgaatgc aattacagcc gcgccgctcg taatcaagaa taaagtgatt atcggagtgg
     7981 cggggggaga tttttcaagt cgcggataca tcgaagcctt cgacgcgcaa accggtgaac
     8041 gcgcgtggca cttcgatacg attccaatgc cgggcgagcc gggcagcgac tcctggccca
     8101 acgccaacgt tgcggcgcgt ggaggaggcg cggtttgggt aaccgggagt tacgacccat
     8161 cgctcaatct cgtttattac ggcactggca atccgaatcc tgattattac ggcgatgatc
     8221 gcgagggcga caatctgtac acctgctcgc ttgttgcgct cgatcttgac acgggaaaat
     8281 tgcgttggca tttccagttc acgccacacg atgtgcatga ctgggactcg gcgcacgtgc
     8341 ctgtgcaggc tgagctaaag ataggcgggc agccacacaa agtcatcatg gtcgcaaacc
     8401 gcaacggctt cttttacacg ctggaccgcg aaactggaaa gctgctggtt gcaaaaccat
     8461 tcatcgacgg ctcgaattgg gcaaaggaaa ttggcaagga cggacggcca attgttctcg
     8521 acaacaacgg aacagcggat aaatgtttgc ccgataatca cggcggcaca aatttccagc
     8581 cgccaacgtt cgatcccgaa cttggattat tttttgtaac cgcacacgaa acctgcgcca
     8641 cctggcaagg agtaaaaccg acggagccca tcgagatggg cgttcgcgta ccgagcggcg
     8701 gacgcgtgtt agttgagaac cacaaccagt tttccgcgct gcgagcgatc gatccgacga
     8761 ccggccagcg caaatgggag cacaagtacc gcgattaccc ttccaccgtc gctctcgatt
     8821 tgacgggcgg cgtgatgtcc acggcgtccg gtctcgtatt caccggagac aacgaaggat
     8881 atttctacgc gtttgaagga gccacgggaa aagagctgtg gaaatttcaa accggcgcgc
     8941 ccgtgtgggg ctcagcctct gttacttaca tgctcgatga caggcagtgg gtggtgacta
     9001 cggcagggtt gacgtttacg gcgtttgcgc tgccagccac gtcgcgttga ggctcgcctg
     9061 gggctgcctg tttgttggtg gcactgtcgt cccgccagcg ctactgtcct gtcgcgatcg
     9121 ctgcaatttt tgcagcgtct gcatctgaga attgattgcc gcgccacacg atgtgcaaat
     9181 ctggcctcac tagcaacaaa tcatattcat aaatttcgcg cgcgcgctgg ttggaaatat
     9241 ccagcacttc gagcggcgca cacgcttcga gaaacaatcg ttccaagcgt gaagtatcgg
     9301 ctttcgttcc gcccagccga agaagcgtat agccggggcc gaggcggtca tgcaacgcga
     9361 tgccatcgct taaccacaca tgtgggagtc gcgcgccggg ccaacttgtg ggcagataaa
     9421 cgagattgtc cggatcaggg ccatctcctg gctcttgcca cacgatgggt gaattcgcat
     9481 agcgatagcc ggattcgatt cctgaaattt cagttacttt gcgctgctcg gtgtcaaaga
     9541 gcgttgctat ttttctgcgc gtggccgcac cttctggtgt gttgtctttt atgtttggat
     9601 gatacgcagc gcgccatccc tggcgcccgg tcatcgcggc gcgagaagct cttacattgc
     9661 gcaagccgat cggtctgcgt tccacctcat aggacgcgag aagctgcgag ccgccccacc
     9721 tggcgagcat tgcagcgagt ttccaggaga gatcgattgc atcgccgacg cccgtattca
     9781 ttccgagtcc gcctgtggga atgaccagat gcgccgcgtc accggcaata aagacgcggc
     9841 cctcgccgta gcgttccgcg cacaaaaggt gctgcgtcca ttcattcacc gaaagcattt
     9901 cgaactcgat gggcattccc agcgatttcc tgaaaatttc cgccatttca gcatcgcttg
     9961 aagcggcagc gtgcagggtc cagtggcgag tgctgtcctg caggatgagg aatggaaaga
    10021 gcggcccctc ggcaatgtga tagtggcggc ccttgcccat gggaatacgc tcgtagagat
    10081 cgtccgcacg gaacagcgcc tggcgcagct tgcgaatatt tccatcgcct tcaaggcgaa
    10141 tgccaagctg tttgcgaacc gtgcttgaac cgccatcgca tccgacgaga tacgcggcgc
    10201 gaatcgtttc gtccattgaa ttattcgatt gtacgaggcc agttacggaa tctaaatctt
    10261 gggtgaagga aactaattcg caaccgaatc taacggttac gctgggcaac gcttccagga
    10321 tcgatcgtaa tagaggctcg agagtgtatt gcgaaattaa ttggtatggt tccaacaggc
    10381 cgccgtcatt gtgcgcggcg atagcggcct tcgcttcggt tacggacggg caaggcaaat
    10441 gtgccaggga tggccccgcc atggagagtg ccagaaaaac gtccatgggc gcgttgcgcg
    10501 gcaagcctgc gtcacgcacc cgctcggaga ttcccaagcg gcgataaatt tccatggtgc
    10561 gcgcgttgca gcgctccatt tttggaagct gaatggaagt tgtgttgcgc tcgatcaacg
    10621 tgcagcgcac gccacgaaga cccaggtcca gagcgagagt aagtcccacg gggccagcgc
    10681 caacgatgag gacttgagtg tctgccactc gcttggagat tccttcgttt tgaatttgtg
    10741 gcgccgcatc ttggcggcgg gttttgtttg gtggcgatta tattcggaaa accgccggcg
    10801 ggacgccggc gttcatggaa tttatcggct tcgtgccgcg gcgtttgccc ggactctgtc
    10861 atccaaatac gccgtcgccg cacccacatt tttattccag gaatttttca tttcccgttc
    10921 taacagcttg atctggatca gatgtttggg attcatcggc aacgacgggt agtagcaatt
    10981 cgattcgtgc gtgcagaagc agccgtcggc ggcctttttg cggcgggcct gcatggagtc
    11041 ggaaaaccac agctgctgaa aattccaatc gtgctcgcgc aaattgccga cgggttccag
    11101 attttcgcag gacgaaaccg tgccctcgtc gtagatcacg cctccagcgg ttccggcgta
    11161 gcacgtcagc tgagcttgct gcgtttcccg agttttggcg atcaggccgt gcatgtacac
    11221 atccaatgcc gctttgaaaa atccgttgct acccgcgtaa tgattcttga ttgtactgcg
    11281 gcgcgaatct tcatcgatct ttgcagcgag cgcggcatat cgcgcgctat cgatttgcaa
    11341 ttccttttga tccgcaaccg gagggcggat gtaattgaaa ttcaccttgt cgggcttcaa
    11401 ctcaaatttc agaaaatcgt accattcaaa aatcgtgtcc tgattcgagt tcatgaagca
    11461 ggtgcaggtt tgcacgtcga gccgcgggta ttcccttttc atggattgca gctggcgcgc
    11521 cgtatcgatg gcaatgtccc acgaaccggt cttttgccga atcgtgtcgt gctgcggctt
    11581 caggccgtca attcccaagg ccatggtcac attcaggctc gggcattcct tcaaaatccg
    11641 aaaaatgtcg gggaaaattc gcttctgaat ctgcccatta gatgtgatgt acaaagattc
    11701 gagattattg ttcgtgtaaa aagcacgcgc aatttccggt aagtcttttc gcgtaaaagg
    11761 ttcgccaccg gcaaggacca ggacgccgag atttccgccc agcgtttcgg aaatcttttc
    11821 aatttcacgc gtggtcattt gcagtttttt gcgtggcgca tcgtccagct gttcagtaaa
    11881 aaagcaatgc gtgcagcgca tgtcgcatac tgaagtgacc agaatattca gcatggcggg
    11941 tgagaacggc tgcccgagcg ccaatttcgc gtagcgtttt aagaaatcgt tcatggtgta
    12001 cgcagcaaat tcaccgctct aattcagcgc tccagatgac cttacgacag taacattcgc
    12061 gggtcaacga gcgctttctg tggcgcgtta ctgttctgcc aactcgaacg gcaagtctgg
    12121 acgcttctgg gctcgctcct tgcgctgatt cgggattctt cacggcgcga tttcctccag
    12181 gcggcgatcg ctggtttcca ccatgcgggt tgcggcaatc gctccaataa cgctgactcc
    12241 cgcaaacatc aggaaaacgg agtggatacc gtcggcgcca accatgaaac ccacgagcgc
    12301 ggggccaagc gccgacgcga tcctaagcca tgaggtggca aggccagtgc caatcgcgcg
    12361 catacgcgtg ggataaattt ccggcgtgta aagataaaga atcgcgttaa tggagccaat
    12421 tagaccgtag cccaacgtgc cgaaaatcat tacactcatt gagttttgcg cgccagtcat
    12481 cccaagatat gccagcagtc caccacccaa aataaatgct ccgaccgtcc agtttctgcg
    12541 gccgacgcga tcaatgctga acgcgcagat gagcagaacc gcgacttgcg cgatgttcgt
    12601 catggaagcg gcgcgcaacg attgccgcag gcttaagtga taaacagtgt tgtacaggct
    12661 ggccatccaa ttgttcaaac cgttggcgat gaagaaggcg cttgcccaga gcagccagac
    12721 aatcagcgtg cgtccacgat aaaaagtgga aagaagttcc gtccattttc cgcgctgcat
    12781 caaattggga acggataccg cgcgagaatt ttccgctgcg ggaatcctgc gtggagtgct
    12841 ggcttcgatt tgctcaacaa ttttttcggc ttcctggagg cgtcctttgg aaatcagcca
    12901 cctcggcgat tccggaagac gcgcaacgag atatgcgatc actagccccg gaactccgcc
    12961 gaaaagaaaa atggattgcc acccgaaggc gggcacgaac caagcgccca gctgccctgt
    13021 agccatgagc ccgagcggaa aaatcatttc gtagagcaga aaaaatctgc ctctgccatg
    13081 cgcgcgcgat aattcattga tgtacgtggc ggccacgggc atttcgccgc ccacgccaat
    13141 tccctgcgcc agcctgcatg cgagcagggc cgggaaattt ccagccagag cgcagccaaa
    13201 actcatgatg gacatgatgg cgattgcggc cgtcgtgccg cgaatgcgcc ccactttttc
    13261 agctagcgcg ctgaacgcca aggctcctgc aagctgacca acgtagctgg cggaaatgag
    13321 caggccgctt tgcttcggag aaatgtgcca gagtctgatc agtgtaggaa gcgcaaacgc
    13381 gagggataat gcgttaaacg catcaaaaaa agttgcacta cccatgatga ggcgggcatt
    13441 cctgtgccag ccggaaaatg gcacttgctc cattcgcgcg agcaagcttc tcgcgtcggt
    13501 tgcgccgagt tgagtttgcg cgcctttttg caattcggcg ccggccgggc cattcgagga
    13561 attcactgcg tctcacaatc aatccgaaga tgaattgcaa actttttgaa tttggacgaa
    13621 catgcggcat ttttgagaca aagctttttc ccctgtagcg ccaaccttca ggttggcatt
    13681 tttaaattca agcgcatgcc cgcctgaagg cgggcgctac ataacgatct ttaaattcgt
    13741 tccaataacg ccaacagctg ttcggcgatt tggtcgggac actctactgg cgtcaggtga
    13801 cggccaccgg gaataatggt cagcgtcgtg aaagttttaa cctgcaaatc attttccatt
    13861 aagcgtttcc cttaggtggt cttaacgaat ggctccgatg gggtcaaagg cacgttccac
    13921 gcgtcataac cgtagagcca actcgtgtct tcgtctcccc ctttcatcca ggtgttggcg
    13981 ctggagattg cctggatgcg cgaagtgcgc ggtttgcgat gagcctcaaa tcgctggaaa
    14041 gctccgggaa tatcttcccc gctggtctcc gcaagacaac gcgcgaggac agcagcgtct
    14101 tcaattgctg ttgctccgcc ttgtgccata taaggcgtca tgggatgcgc agcatcgccc
    14161 aggagcacaa cgcacccttc gctccatctg tccaacggct cgcgttcgag gatcgcccac
    14221 ttgtggcagt ccgggcatgc ttcaagcacg gcgcggacat cggaatgaaa accttcgtag
    14281 gcctggcgca attctttcac atcgcctttt gccgaccacg attcgcgcgt cagccactcc
    14341 gccggttcgg gtatgctggt cacgaaataa agttcgctgc aatcggcatt cgtgtaatag
    14401 atgacgatgt gacggtctac tccccaccat ttcgtgcgcg aagctccgat atccattccc
    14461 ttgagcaatg cggctggaaa aatagcgcga taggcgatgc gccctttgtg gatgggctca
    14521 tcagcgccaa ccataatttc gcgcacaacg gagtgaacgc catccgcgcc cactacggca
    14581 tcggcatggg cgcgacttcc gtccgcgaac gtgagagtga tttgtcccgc gctttgctcc
    14641 agcccgacca attttttatc gagatggact atttcttccg gaacaaccgc cgctaaagcc
    14701 ctgtgcaggt ccgcgcggtg catgcaaagg tacggcgcac cgaaaagggt ttccggcatt
    14761 ggcaactcgc gaatcacttc gccggtgtcc catttccgat tcaaatgcga atagggcttg
    14821 aaagaagttg caatcacttt ttcttcaaca ccaattttgc ggaggacttt cattgagttg
    14881 ggcatcatct gaatgcccgc gccaattcgc aggaagcgcg gcgcttgctc gtagacgtga
    14941 acgtccatgc cgatttggcg caacgtggcc gcagtggcca taccgccgat tccggcgccg
    15001 acgatagcta tcgaaagctt tttcgcgccc atctactggc tcctcaagat cagggctacc
    15061 gctaacttta aaatcgcgtg agccgggtct tgtggcgcag gcgtctcgcc ggcggttttt
    15121 atgatgtggt gccgatcgaa aacccgtcga cggggcaccg gcgccacaaa aacacttcgc
    15181 cggttatgcc gccggtgaag cctggccttt aatttgggtg cgatgcatca cgcggcgcga
    15241 gcttgcatca aagggatcgc ggcggtgcat ggtgcagcgg ttgtcccaga acacgagatc
    15301 gccaacgcgc cactcgttat gccaggcaat ttcttcgcga tccatatagg accacaactc
    15361 gtcgagcagc gcttccgaat cccgtaacga taatccctga atgtacgcat tgcgtcggcg
    15421 ccccagatac aggccgcgcc gcttggtttc tgcatgcgtg caaacgagcg gatgaaatgt
    15481 gccgggagac tgaacgggat catcgaccgc agtcactccc tgccgaacat agcctccgct
    15541 gttatacgtt ccatcatgct tcagcaattt gccttcgaca cgcttcttca gcgcatcggg
    15601 caggatttcg taggcgcgat acatgctgga gaagtacgtg ttgccgccta atggaggaac
    15661 ttccagcgca tacaggatgc tcgccttcgg cggatcggga agataggaca tgtccgtatg
    15721 ccatgtcgcc tcgcccgcgc ccaggcttcc gatgggcacg ccgttttcca tcacgttgga
    15781 caccacataa atttcgggat gtccctcgac aaagcggcgg cccgtttctt gaatcggcgc
    15841 caaatccaag tctccgaagt gtttgctgaa ggcgatgaga tcgtcatccg tcaatttctg
    15901 gttccggaaa aggagaacgg aatggtccag ccaggcctgg tgaatgctct caaaattgtc
    15961 agcactcact gcgcgcaaat cgactccctg aatttcagcg cctagagctt ttcccgaggg
    16021 aacaactgtg acgcgagaag tgctgctcgc tctt
//
LOCUS       DASZMB010000086         2796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4479440, whole genome shotgun sequence.
ACCESSION   DASZMB010000086 DASZMB010000000
VERSION     DASZMB010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4479440"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VG272_04035"
     CDS             <1..1015
                     /locus_tag="VG272_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWF12882.1"
                     /translation="MRALGLTAGAAFAAGAVPELLTVSAATAQEAVPGRLSLKAVSYN
                     HINYQVQDYAKVRDFYINVFGMKDVWDDGKQCSVECGSPPNAIYIRPLNKPLDRAPGN
                     WSAQMGQGNVDHFAFSLENFELEPVRAELVRRGLEPKGDGEYAWSIKDPDGTVIQICA
                     VHGVYPGAAAPNAKESNGLKNLSSIPGPDGKGFKAVAVSHLVYNVADVDRARDFYMNL
                     LDMKLVYYKPGGVFGVDEPGGPVCFLKFGENYFYLRQSQHPDKKPYVAHFTLTVENFD
                     QAVVKAQLEKLGYKPTPDSKYAWSIHDPAGMRTEVAGKGVPEHVGGDCGGSNAKCPGG
                     EDK"
     gene            1069..1950
                     /locus_tag="VG272_04040"
     CDS             1069..1950
                     /locus_tag="VG272_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HWF12883.1"
                     /translation="MKKQFGFVVLAVVGILHSSAAWAQSSGKTADRLYVLDCGTGHAP
                     DESRWTVGFNVGKPIDISVSCYLVRHGNEYFLWDTGISDAVAAMPDGWYPSNNPATDI
                     HWSRAKTLESQLAGIGVKPSDIKFVGISHTHPDHIGNAELFPAVPFLIQKTEYDYYFA
                     PGKTGIAKPPSDSRPTFLKEHPATMVQEDLDVFGDRSVMLIFTPGHTPGHQSCMVHLA
                     KTGWILLTGDAVHLQDNWDNRRVPYFTTMPAEQKVQTQLSMQRMADLLSFYHAQLWIN
                     HEKAQSDKLKHAPAYYE"
     gene            1972..>2796
                     /locus_tag="VG272_04045"
     CDS             1972..>2796
                     /locus_tag="VG272_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12884.1"
                     /translation="MMKPRIAILKIIAGILVSAFSFMCAAPGARAQQNATSAADALQK
                     AHVQDRLAESLSATHKGTAPGFVLDPAWPMPLPHHWVIGDIGGIAVDKHDHIWVLHRP
                     RSVSSTDSGMQPAAGTDAKGKPISAMGFPRPNTRINQCCMPAPSVLVFDKAGKLLQAW
                     GGPGDEDFSRKKCRAQDGCTWPAREHGIYVDQNDFVYIGGNGQARNFHGQFLWAPSFG
                     NDSHILKFKTDGTFVYQIGTPGAKGPNSNDTNGGVNGTPEPFWPADTVVDAKNNLMY"
BASE COUNT          657 a          758 c          795 g          586 t
ORIGIN      
        1 catgcgcgcc ctgggcctca cggcaggtgc tgcgtttgcc gccggcgcgg tgccggaact
       61 gttgacggtc tccgcggcaa ccgcccagga ggcggtaccc ggccgattgt ccctgaaagc
      121 agtgtcctac aatcacatca attatcaggt tcaggattac gcaaaagtac gcgattttta
      181 cataaatgta tttgggatga aggacgtgtg ggatgacgga aagcaatgtt ccgtggaatg
      241 tggcagcccg ccaaatgcca tctacattcg cccgctgaac aagcccctcg accgcgcccc
      301 cggcaactgg tccgcgcaga tgggccaagg caatgtcgat cactttgcat tttccttaga
      361 aaactttgag ctggaacccg ttcgcgccga actcgttcgt cggggcttgg agccgaaagg
      421 agacggcgaa tacgcgtggt caatcaagga tcctgacgga accgtcattc aaatctgcgc
      481 ggttcatggc gtttatccgg gggcggctgc gccgaatgcg aaagaatcca atggcttgaa
      541 aaatttaagc tcgattccag gaccggacgg aaaaggtttc aaggccgttg ccgtgagcca
      601 tctggtgtac aacgttgccg acgtcgatcg cgctcgcgac ttctacatga atctgctgga
      661 tatgaaattg gtgtattaca aaccaggcgg tgtgtttgga gtggacgaac ccggagggcc
      721 cgtctgcttc ctgaaatttg gcgaaaatta tttttatctg cgccagagcc agcatccgga
      781 caagaaaccg tacgtcgcgc actttacgct gacggtggag aatttcgatc aggccgtagt
      841 caaggcgcaa ttggagaaac tcgggtacaa accgaccccc gacagcaaat acgcgtggtc
      901 tattcacgat ccagcgggca tgcggacgga agtggcgggt aaaggcgtac cggaacatgt
      961 tggcggcgat tgcggcggaa gtaacgcgaa atgtcccggt ggggaagaca aatagatcgg
     1021 gcacaaatgt gggaagcagt tttctagtct cacaaagcgc gaggagtgat gaaaaaacaa
     1081 ttcggtttcg ttgttttggc tgttgttggg attttgcatt cgagtgcagc ctgggcgcag
     1141 tcaagcggca aaactgcaga ccgcctttac gtgctggatt gcggcaccgg gcatgccccg
     1201 gacgagtcgc gctggacggt tggctttaac gtcgggaaac caatcgacat ttcggtcagt
     1261 tgctatctgg ttcgccacgg caatgaatat tttctatggg acacgggtat ttccgatgca
     1321 gttgcggcaa tgcccgatgg atggtacccg agcaataatc ccgcgacgga cattcactgg
     1381 agtcgcgcca agacgctcga gtctcaactc gccgggattg gcgtcaagcc ttcggacatc
     1441 aagtttgtag gaatttcaca cacgcatccc gatcatatcg gcaatgcgga attatttccc
     1501 gctgtgccgt tcctcatcca gaagaccgaa tacgattact attttgcgcc cggaaaaacg
     1561 ggaatcgcga agcctccatc ggactcgcgg cccactttct tgaaggaaca tcccgcgacc
     1621 atggtgcagg aagatctcga tgtgttcgga gatcgctcgg tgatgctcat cttcactccc
     1681 ggccacacgc ccggacatca gtcctgcatg gtccatctcg caaaaactgg ttggatcctg
     1741 ctgaccggcg acgcggttca cttgcaggat aattgggaca accggcgcgt tccgtatttc
     1801 accacaatgc ctgcggagca aaaagtgcag acgcagctat ccatgcagcg catggcggac
     1861 ctgctttctt tttatcacgc gcaactctgg atcaatcacg agaaggcgca gagcgataaa
     1921 ctgaaacacg cacccgcata ttacgaataa gtgtaacttc cagggagata catgatgaag
     1981 ccaagaatcg cgatactcaa aatcatcgcg ggcatacttg tttctgcgtt cagcttcatg
     2041 tgcgctgcgc ccggtgcgcg cgcgcaacaa aacgcgacca gcgccgcgga cgccttgcag
     2101 aaggcgcacg ttcaagaccg cctcgcggaa tcgctctccg cgacgcacaa gggaaccgct
     2161 ccaggctttg tgctggatcc cgcgtggccc atgccgttgc cccaccactg ggtgatcgga
     2221 gacatcggcg gaattgccgt cgacaagcac gatcacatct gggtgctcca ccgccctcgc
     2281 tccgtcagtt ccacggatag cggaatgcag cctgcggccg gcacggatgc caagggcaag
     2341 ccaatcagcg cgatgggatt tccacggcca aacactcgaa ttaatcagtg ctgcatgcct
     2401 gcgccgtcgg tgctcgtgtt cgacaaagcg ggcaaattgc tgcaagcctg gggcggtcct
     2461 ggcgatgagg atttctcacg gaaaaaatgc cgcgcacaag atggttgtac atggcccgct
     2521 cgcgagcacg gcatttatgt ggaccagaac gattttgttt atatcggcgg aaatgggcaa
     2581 gctcgcaatt tccatggaca atttctgtgg gccccgagtt tcggtaacga ttcgcacatc
     2641 ctgaaattta aaacggatgg cacgttcgtc tatcagattg gcacgcccgg cgccaaggga
     2701 ccgaacagca acgacacgaa tggcggcgtg aacggaactc ccgaaccatt ttggccggcc
     2761 gatacggtgg tcgatgcaaa gaataattta atgtac
//
LOCUS       DASZMB010000087         8112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2564171, whole genome shotgun sequence.
ACCESSION   DASZMB010000087 DASZMB010000000
VERSION     DASZMB010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8112
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2564171"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VG272_04050"
     CDS             <1..565
                     /locus_tag="VG272_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12885.1"
                     /translation="LASPERALRFAFDYYSGVPNLEEPPVHLIQTSVADPTRAPAGMH
                     TVKVVGYHPYDLKEGPHHWDNIKEHVADETLKYIQLFSPTLTHDKILEREVMSPLDLE
                     RMNPHNWHGSCHAGGSGPAQAGQNRPMPGWANYKMPIPGLYQTGGTTAPGGSVSGQPG
                     RNAAIVMLKDLGTSIEQVAAKKETSKS"
     gene            622..1212
                     /locus_tag="VG272_04055"
     CDS             622..1212
                     /locus_tag="VG272_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HWF12886.1"
                     /translation="MKGITKLSVSTLSAAALFAVLFFFAQSARAQQTAAAAAPEIKQE
                     VPDNPVEHAPPDQPLPYSHKTHAGTLGLPCMSCHTNPEPGNMMTFPTAAKCMSCHRAV
                     ATNKPAIRKLAELSKSAQPIPWVRVYKVLPGVNWTHRKHLEAGLKCQMCHGQVQELDR
                     MAENTSVTTMGVCLKCHQDHGAPVVCSTCHSWPPAN"
     gene            1560..2246
                     /locus_tag="VG272_04060"
     CDS             1560..2246
                     /locus_tag="VG272_04060"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWF12887.1"
                     /translation="MKSSSQILLGLLLFLGFGIVAKYYLHAQSKQLPSETASFPKDIY
                     PDTGNRLPAIKREDLDDAGKKLFDAKGPSDTYGPGSIRLYSLPVEQYAETTNDFLRQK
                     AGIDPRIVELVILVTAREEDCAYVWNAHEPQGLKAGLPKETVDIVRDRKPLVGVEEKY
                     AAVIQLGREVLEKHHVESATAAHALKLFGKEGLVNLVSLMGDYASTEILLNAFDQHVR
                     PTEKSLLPLP"
     gene            complement(2384..3370)
                     /locus_tag="VG272_04065"
     CDS             complement(2384..3370)
                     /locus_tag="VG272_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWF12888.1"
                     /translation="MSNCLRPHALTISFVLIFVCITSAISGIPAKNPQETAPMGHNQS
                     QPVPLVQENKTIKVSEHVYEIPDGRVNLVPNIGIIVGSRATLIVDAGMGPKNGEAVLR
                     EVAKVSKNPELFFTTTHFHPEHMTGGQAFPADTIVIRPEAQQEEVDRKQPEFIRNFSR
                     RTPEMKALLQDVKPRPPDIVFDHEAKLDLGGVTVRLLWLGAAHTRGDEFVLVEDDSVL
                     FTGDAVINRFFPIFPDADASGKNWLSILDKLDALHPRIVVPGHGEAGEASLIATEREY
                     LQAVQQRVAALKSQGKSVDETAKLLTAEFRTKYPNWENPGWRRCSRAILRGG"
     gene            3539..4249
                     /locus_tag="VG272_04070"
     CDS             3539..4249
                     /locus_tag="VG272_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWF12889.1"
                     /translation="MYKCVDPVRQNPRFSFGKEILVQHVLVYGDSLSWGIVPHTRKRL
                     PFDERWPGVLENKLKELGYPLRIIEDCLNGRRTVWDDPFKPGRNGLIGLAQCMERNSP
                     LALVMLMLGTNDFQFSHPHNTAWAAGQGIATLVHEIKQAPIEPGMAIPPILVICPPPI
                     TAPQGPLAEKFSGAEKRCAGLADAYAAICAEVGCHFFDSASAITSSSSDGIHLDAEQH
                     AVLGNALVGVVATVLAVV"
     gene            complement(4270..5049)
                     /locus_tag="VG272_04075"
     CDS             complement(4270..5049)
                     /locus_tag="VG272_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWF12890.1"
                     /translation="MSEEVLYRANDGVGRITFNRPEARNAFTFRMYERLVEICKQACA
                     DPAIRVLVLSGVDETAFAAGTDISEFKKWSSAQDALDYEARINAMLDGIERIPLPTIA
                     AVSGACTGGGSTIASCCDIRIGAANARFGVPISRTLGNCLSMANYVRLVALLGQARVK
                     DIIFTGRLVEAEEARTIGLLSEVLPDFASLQTRADELARTLASRAPLTLRVTKEALRR
                     IKEKMTPEEDSSMILACYMSHDFQEGMDAFLNKRKPKWTGA"
     gene            complement(5046..6287)
                     /locus_tag="VG272_04080"
     CDS             complement(5046..6287)
                     /locus_tag="VG272_04080"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HWF12891.1"
                     /translation="MNDLKRLGPLDGLKVVDLSHIMAGPTCSMLLADMGADVIKVEKI
                     PGGDDSRRMVPPKIAEESAAFLIMNRNKRGIALNLKTEQGREVLTRLLKNADVLIENY
                     RRGTMEKMGFGYETLHALNPKLIFCSISGFGRTGPYADRGGFDLVAQGMTGLMSITGE
                     KQGGPPMKSGAPVTDITAGILACVGILAALHARESTGQGQMVDTSLFEAGITHTYWHS
                     AICFATGKAPGPMGSAHPLNGPYQAFPTSDGWITVGAANQDNWLRLLNALEAPQLGDD
                     PRFANNAQRMTNLSVLVDALTPLFKRRTVADWLARLESAGVPAGPVLDIAQMHADPQA
                     ISREMIVETAHPVAGKVKAIGLPIKFSETPGGVNRPAPVFGEHTREVLREQDYSEKEI
                     DHMVEEGAVQTPDSTKKGATR"
     gene            6648..7850
                     /locus_tag="VG272_04085"
     CDS             6648..7850
                     /locus_tag="VG272_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12892.1"
                     /translation="MRRKFFPAGMCLVMLASVSIFSLPVKAQTTYNVNAGGETRDQSV
                     QANAFLPNEVWLLEGDSIKWTFAPKNEVHTVTLLTPGQIRPSAPPPAGPPFAVQGVNC
                     GPAASYDGSACVSTPAGFNGGATFTVKFPKAGNYKVVCLIHTDMTGTVHVLLNNAANS
                     ALIKSQRYYDDQGQDEAEAILVDNDGSSVDRDGWRNRRNSGHTVTAGIGEIVATGGGL
                     QYRAVVRFLPGEIHIHAGESIVWTNLDPTEPHTVTFGTEPAGFVPTLYSLPPAVLLPA
                     GPPIPNPPAPCSSTVTTNCTDPGTGTVIATVNCTTTPPATAGTTPCKAAFNPSELSVN
                     RPPPPFDAFSFINSGFLQASVPDRTGTAQTPPGTTRIRITFPNKGTYYYHCALHDVDG
                     MNGAVVVE"
BASE COUNT         1734 a         2288 c         2268 g         1822 t
ORIGIN      
        1 gttggccagc cccgagcgcg cgctgcgttt cgcatttgac tattactctg gcgtgccaaa
       61 tctggaggag cctccggttc atctcatcca gacttccgtt gccgatccca cccgcgcgcc
      121 tgcggggatg cacaccgtta aggtagttgg ctaccacccg tacgatctga aagaaggccc
      181 gcatcattgg gacaacatca aggaacatgt ggccgacgaa acgctgaaat atattcagct
      241 tttttctccg actcttacgc acgataagat tctggagcgc gaagtgatga gcccgcttga
      301 cctcgagcgc atgaatccgc acaactggca tggcagttgc cacgcgggag gttcgggccc
      361 cgcgcaagcc ggtcagaatc gtccgatgcc gggctgggcg aattacaaaa tgccgattcc
      421 cggcttgtat caaaccggcg gaacaacggc tccgggcgga tcggtttccg gccagccggg
      481 gcgcaatgct gcaatcgtga tgctcaagga tcttggcacc agcattgagc aggtagcggc
      541 caagaaagaa acgagcaaat cgtaggaaaa gtttttcaaa actatttttt cgtgcgttcg
      601 caaagcaatc taggagggga tttgaaaggg atcacgaaac tatcggtcag cactctaagc
      661 gccgccgcgt tatttgctgt actgtttttc ttcgcgcaaa gtgcgcgggc acaacaaacg
      721 gcggccgctg cggcgcccga aattaagcag gaagtccccg ataatcccgt ggagcatgcg
      781 ccgcccgacc agccgcttcc ctacagccac aaaacgcacg cggggacgct cggcttgccg
      841 tgcatgagtt gccacaccaa tcctgagccc ggcaatatga tgacgtttcc caccgcggcg
      901 aagtgcatgt cgtgccatcg cgccgtcgcc accaacaagc ccgcgatcag aaaactcgcg
      961 gaactttcca aatcagctca gccgattccc tgggtacgcg tctataaggt tctcccgggc
     1021 gtgaattgga cgcaccgcaa acacctcgaa gccgggctga aatgtcagat gtgccatggg
     1081 caagtgcagg aactggatcg gatggctgag aacaccagcg tgactacgat gggcgtctgc
     1141 ctcaaatgcc atcaggatca cggcgcgcca gtcgtatgct cgacgtgcca ttcctggccg
     1201 cccgcaaatt agaatagagt tgaaagccac ccgagaatga atttttgtga atcgcggctt
     1261 cacacgttgc ggggaacaat ctgtcatttc gagcaatcca tttcagctga ggaatctctc
     1321 ttcgttttgg actcaacatc gaagagagct tcctcgctac gttcgcaatg acgatcagtc
     1381 gtcgagtagc acagccactc tcggctgtgt gcactttgca aaacccacac aggcaagagt
     1441 gcctgtgcta ctcaacattc gctcaagcag ttttgccgtt gcctgttcag ccgcggcata
     1501 aattccgctg atggattcgg ctttagccgc tgaaggccag tcgaggtcgg gggacactcg
     1561 tgaagtcatc atctcaaatt cttttgggcc ttttgctatt ccttggattt ggcatcgtgg
     1621 cgaagtacta cctgcacgcg cagtccaagc aattgccaag cgaaaccgct tcattcccga
     1681 aagatattta tccggatacc ggcaatcgcc ttccggcgat caaacgagag gacctcgacg
     1741 acgccgggaa aaaacttttc gatgccaaag ggccttccga tacgtatggt ccgggctcga
     1801 ttcgccttta cagcttgccc gtggagcaat atgcggaaac cacgaacgat tttctacggc
     1861 aaaaggctgg aattgatccg cgcattgtgg agctggtgat tctggtaacg gctcgcgaag
     1921 aggactgcgc ctatgtgtgg aacgcgcatg aaccacaagg tttgaaagca ggtttgccca
     1981 aggaaaccgt cgacatcgtc cgcgaccgca aaccattggt gggagtggag gaaaagtacg
     2041 ctgcggtgat tcaacttggt cgcgaagtgc tggaaaagca ccacgtcgag tccgcgaccg
     2101 ccgcccacgc attgaaattg ttcggaaagg agggtttggt caatctggta tctctgatgg
     2161 gagattatgc ttccacggaa attcttctga acgctttcga tcagcatgtc cgcccgactg
     2221 aaaaatccct gttgccactt ccctaacctt ttattaagcg ggcgtcttgg gtaggacagg
     2281 cttcagcctg tctatttttg acgctctatg cgaacgcagg aatcaaagga agacaggctg
     2341 aagcctgtcc tacttgaggc gactgagcca cagaccaggt tagtcaccct ccgcgtaaaa
     2401 tcgctcgact gcatcggcgc cagcccgggt tttcccaatt gggatatttt gtacggaatt
     2461 ccgccgtcag caatttcgcg gtttcgtcca ctgattttcc ctgcgatttc aaggccgcga
     2521 cgcgctgctg caccgcctga agatattcgc gctccgtcgc gatcagcgac gcttctcccg
     2581 cttccccatg tccgggaacg acgatccgag gatgcagcgc gtccagcttg tccaaaatgg
     2641 atagccagtt ttttccgctc gcgtcggcat ccggaaaaat ggggaagaat cgattaatca
     2701 ccgcgtctcc cgtaaacaag acgctatcat cttccaccag aacaaactcg tcgccgcgcg
     2761 tgtgcgccgc acccagccac agcagccgca cggtcacgcc gcccaaatcc agtttcgcct
     2821 catgatcgaa gacgatgtcc ggggggcgcg gcttcacatc ctgcaaaagc gctttcatct
     2881 ccggcgtcct gcggctgaaa ttgcgaataa attccggttg cttgcgatcc acttcctcct
     2941 gctgcgcctc ggggcgaatc acaatcgtgt ctgcgggaaa tgcctgtccg ccagtcatgt
     3001 gttccggatg aaaatgtgtt gtcgtgaaaa ataactcggg attcttgctg actttggcaa
     3061 cttcgcgcaa aactgcttcc ccgtttttcg gtcccatccc ggcgtccacg atcagcgtcg
     3121 cccggctacc cacgatgatg ccaatgtttg ggacaagatt tacgcggcca tcgggaatct
     3181 cgtaaacgtg ctccgaaact tttatggtct tgttttcctg cacgagtggc acgggttgcg
     3241 attgattatg gcccatgggc gccgtttcct gcggattttt cgccgggatg ccggagatag
     3301 cgctggtgat gcaaacgaaa ataaggacga aggaaatggt gagcgcgtgt ggacgtaagc
     3361 aattgctcat ggttggctct ccttaatacg gtttggccga gatgggtcct gacgattgcg
     3421 ctgtgggcga atgctatggc cgccgccgag tgttgtcaac cgtcgcgaag cacagtcaga
     3481 cttcggccaa tgccgccaag caagctggca cattttagtt ttacggggat tgggttgagt
     3541 gtataagtgt gtcgacccgg ttcggcaaaa cccaagattt tctttcggaa aggaaattct
     3601 cgtgcagcat gttctggtgt acggcgattc gttgtcctgg ggaattgttc cccacacacg
     3661 caagagactg ccttttgacg aacggtggcc gggcgtgctg gaaaataaac tgaaagagtt
     3721 gggttacccg ctgcgaatca ttgaagattg cctgaacggc aggcgcacgg tgtgggacga
     3781 tcccttcaaa ccggggcgca acggcctcat tgggcttgcc caatgcatgg aaagaaattc
     3841 cccactggcg ctcgtgatgc tgatgctggg cacgaacgat tttcagtttt cgcatcccca
     3901 caataccgct tgggcagccg gtcaaggaat tgcgacgctc gtccacgaga ttaaacaggc
     3961 gccaattgag cctggaatgg cgatcccccc gatcctggtg atatgcccgc ccccgattac
     4021 ggctccgcaa ggcccgcttg cggaaaaatt ttccggcgcg gaaaaacgtt gcgcggggct
     4081 ggccgacgcc tacgccgcga tctgcgcgga ggtcggttgc catttcttcg attcagcgtc
     4141 cgcgatcaca agcagcagtt ccgatggcat tcacctcgat gccgagcagc atgcagtatt
     4201 gggaaatgcc ttggtggggg ttgtggcaac cgttttagcg gtggtgtaag cgcgggacac
     4261 ttctaacttc tacgcgccgg tccactttgg tttgcgtttg ttcaggaacg cgtccatgcc
     4321 ttcttgaaaa tcgtggctca tgtagcaggc aaggatcatg ctgctgtctt cctcgggcgt
     4381 cattttttcc ttgatgcggc ggagggcttc tttcgtgacg cgcagggtaa gaggagcgcg
     4441 tgaagccaag gtgcgcgcga gttcgtcggc gcgcgtttgc aatgaggcga agtcgggcag
     4501 cacttcgctg agcaaaccaa tcgtgcgcgc ctcttccgct tccaccaatc ggcccgtgaa
     4561 aataatatcc ttcacacgcg cttgaccaag caaggccacg aggcgcacat aattcgccat
     4621 cgacaagcaa ttgcccagcg tgcgggatat gggcacgccg aaacgcgcgt tggccgcgcc
     4681 gatacgtata tcgcaacacg atgcaattgt gctgcctccg cctgtgcagg ctcccgaaac
     4741 ggcggcaatg gtgggaaggg ggattctttc tatcccatcg agcatggcgt tgatgcgcgc
     4801 ttcgtagtcg agtgcgtcct gcgcgctgga ccatttctta aactcggaaa tgtcggtgcc
     4861 cgcggcgaac gccgtttcat ccacgccgct gagcacgagt acgcggatcg cgggatcggc
     4921 acacgcttgc ttgcagattt ccacgaggcg ctcgtacatg cgaaacgtaa acgcgttgcg
     4981 cgcctcggga cggttgaaag tgatgcggcc tactccgtcg ttcgctcggt ataaaacttc
     5041 ctcactcatc gtgtcgcgcc tttcttcgtc gagtccggcg tttgcactgc gccctcttcc
     5101 accatgtgat cgatttcttt ttcggaatag tcttgttccc gcaaaacttc gcgcgtgtgc
     5161 tcgccgaaga cgggcgcagg cctgttcacg ccgcccggag tttccgaaaa tttaatgggc
     5221 aggccgatcg cttttacctt tcccgcaacc ggatgcgcgg tctcgacgat catttcgcgc
     5281 gagatggcct gcgggtctgc gtgcatctgc gcaatgtcca gcacgggacc tgcgggaacg
     5341 cctgcggatt ccaagcgcgc cagccaatcg gccacggtgc ggcgtttaaa aagcggcgtc
     5401 agggcatcga ccagcacgga aagatttgtc atgcgctgcg cgttattcgc aaaacgagga
     5461 tcgtcgccca gttgtggagc ttccagcgcg ttcaggagtc gcagccagtt atcctgattt
     5521 gccgcgccca ctgtgatcca accatcggaa gtgggaaatg cctggtacgg accattcaaa
     5581 ggatgcgcgg aacccatcgg gccgggagcc ttcccggtgg caaagcagat tgccgaatgc
     5641 caataggtgt gcgtgattcc cgcttcgaac agagatgtgt ccaccatctg cccttgccct
     5701 gtagattcgc gggcgtggag cgccgccaga attccgacac aggcgagaat tcccgcggta
     5761 atgtccgtga ccggggcacc ggacttcatc ggcgggcccc cttgtttttc gcccgtgatg
     5821 ctcatcaatc cggtcatccc ctgcgcgacc aagtcaaagc cgccgcgatc cgcgtaggga
     5881 ccggtgcggc cgaatccgga gattgagcag aaaattaatt tgggattcag ggcatgcaga
     5941 gtttcgtatc cgaaacccat tttttccatc gtgccgcggc gataattttc gatcagcacg
     6001 tccgcgttct ttagcaagcg ggtcagaacc tcgcggcctt gttccgtttt caggttgagg
     6061 gcgatgccgc gcttgttgcg gttcatgatc agaaacgccg cggattcctc cgcgattttg
     6121 ggcggcacca tgcggcggga gtcatcaccg ccaggaattt tttctacctt gatgacatct
     6181 gcgcccatat cggcgaggag catcgagcag gtaggcccag ccatgatgtg ggagaggtcc
     6241 acgactttca gtccgtcgag cggtccaagt cttttcagat cgttcaccgt attgagctca
     6301 tttcattctt aatttacgag aagccagttc acaattgtat gaacctactt ggccgataaa
     6361 gcttcagggg ccaaggctcc gacatttgga ttacggaaat gtcgcggctg aaaccgcgac
     6421 ctacaaagcg cgtttgtagg tgccggcttg tggtggtcga acttccgcgg aagcgagttc
     6481 tgtcactccc agtactcacc taaaatggcc ctcccgcaaa gcgattttaa cttgacacgc
     6541 tgccgcgtgc cgctctatga acacatgtcg agaaaattcc acctgttgaa gataaactat
     6601 cccgcttcgg tgggacgaaa atatttgaaa attcttgtgg aggatgaatg agacgcaaat
     6661 ttttcccagc agggatgtgt ttggtaatgc tcgcgtctgt gtcgattttt tcattgcccg
     6721 tgaaggcgca gactacgtac aacgtgaatg ccggaggcga gaccagggat caatccgtgc
     6781 aggcgaatgc ttttttgccg aatgaagtat ggttactcga gggagacagc attaagtgga
     6841 ccttcgctcc caagaatgaa gttcacacgg ttacgctgct gacgcccggc caaattcggc
     6901 cctcagcgcc cccgccagcc ggacctccat tcgcggtgca gggtgtgaac tgcggacccg
     6961 cggccagcta tgacggtagc gcgtgcgtat ccactcctgc gggatttaac ggtggtgcga
     7021 ctttcactgt gaaatttccg aaagcgggca actacaaagt tgtctgcctg attcacacgg
     7081 acatgaccgg cacggtccac gttctgctca acaacgcggc caacagcgcg ctcatcaaga
     7141 gccagcgcta ctacgatgat cagggacagg atgaagcgga agcgattctc gtggacaacg
     7201 acggcagcag cgtggatcgt gacggatggc gcaatcgccg gaatagcggt cacacagtga
     7261 ctgcaggcat cggagagatc gtagcgacgg ggggaggctt gcagtatcgc gcggtcgtgc
     7321 gctttctgcc gggagaaatt catattcacg ccggcgagtc catcgtgtgg acgaatctcg
     7381 atccgactga accgcatacg gttacgtttg gcaccgagcc cgcgggattt gttcccacac
     7441 tctattctct acccccggct gtgttgttgc ctgccggccc gcctattccg aatccgcctg
     7501 ctccgtgctc atcgacggtc acgacaaatt gtaccgaccc gggcaccggc acagtgattg
     7561 caacggtgaa ttgcaccact actccgcctg ccaccgcggg caccacgccg tgcaaggccg
     7621 cttttaatcc gtcggagttg tctgtgaatc gcccaccgcc gccatttgat gcattcagtt
     7681 tcatcaactc cggattcctg caagcgagcg tgccggatcg aaccggaacg gcgcaaacac
     7741 cgccgggaac gacgcgtatt cgcattacgt tccccaacaa gggcacgtat tactatcact
     7801 gcgcgcttca tgacgttgat ggtatgaatg gcgcggtggt tgttgaatag tttccggatc
     7861 atgcaaatga agtggcgagt gttggtcagg accttcctgg ttttggtttt tctcttggga
     7921 atttgtcacc gagtggcggc tcaggcgtcg aaaaataatc ctgggtccgc ctcttccggt
     7981 ggcgctgaac caaaataccg gggcggactc gtttcaccgc ccctgtcaaa accaaaattc
     8041 gcgctaaccg atacctcggg agcttccttc gatttttcag ctaagacgca cggatatgtc
     8101 acccttctgt tt
//
LOCUS       DASZMB010000088         6455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3109895, whole genome shotgun sequence.
ACCESSION   DASZMB010000088 DASZMB010000000
VERSION     DASZMB010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3109895"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /gene="nuoE"
                     /locus_tag="VG272_04090"
     CDS             complement(<1..1109)
                     /gene="nuoE"
                     /locus_tag="VG272_04090"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HWF12893.1"
                     /translation="MNLPPQLEAKFQTLENRYPVKRSALIPMLLYAQDQFGFVSDEMI
                     LEIAQRLGLNTLQVTETMEYYSMLRRKRAGKYHVQVCTNVSCMLRGGNQIYDFVQKKL
                     EIGHKETTADGVFSLEEVECLGACTGAPAMQVNYDFYENLTVERTTQIFEALEAGRKP
                     QTSTPITGAVKQRHPAEVPAISRLFGVKDSHKLEVYVANDGYKGMERALKELTPEQVV
                     DEVKKSNLRGRGGAGFPTGMKWSFVPKDSPKPKYILCNADESEPGTCKDRPLMELMPH
                     QLIEGIVIAGRAVNSHQGYVYIRGEYRYVLDIMEEAIAEAYKGGYLGKNILGSGYDFD
                     LVAHTGAGAYECGEESALMESLEGKRGYPRIRPPFP"
     gene            complement(1109..2305)
                     /gene="nuoD"
                     /locus_tag="VG272_04095"
     CDS             complement(1109..2305)
                     /gene="nuoD"
                     /locus_tag="VG272_04095"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HWF12894.1"
                     /translation="MTTIQTAVGPEKTMVLNMGPQHPSTHGVLRVLLELDGENIVKAD
                     VDIGYLHTGIEKTAEALTYSQAITITDRMDYLAPLSNNLAYCLAVEKLLGLEVPKRAQ
                     YIRVLLTELTRIGSHLVWLGTHAIDLGAMSVFLYCFREREELLKIFELVSGQRMMTSY
                     FRIGGLALEPPVGWTDHVEKIVRTFPSRFDEYEELLTKNRIWIGRTQGVGILKAEDAI
                     ALGVSGPSLRGSGVDYDVRKYFPYSSYDEFDFNVPVRTEGDCYARYLCRVGEMRESLK
                     IVRQALEKIPPEGPVKADAPGIVPPQREKMKTEMEALIYHFKIFTEGFAPPPGEVYQR
                     IESPRGELGCFIASDGSPKPYRMRMRAPSFVNLQALQKMVEGHLVADAIASIGTLDII
                     LGEIDR"
     gene            complement(2337..2816)
                     /locus_tag="VG272_04100"
     CDS             complement(2337..2816)
                     /locus_tag="VG272_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWF12895.1"
                     /translation="MTDTPDLEQNPIVAKIRDWRPEAIAELISFRDELTIVIYREHIR
                     PLCEFLRSDSFLAFTYLSDVTGVDRFPIEPRFELNYHLLSLSRRDTVRLRLRLPGEDP
                     LVESVVSVWPTANWHEREIFDLFGIRFDGHPNLHRMLLPDDWEGHPLRKDYPVEGPR"
     gene            complement(2813..3211)
                     /gene="ndhC"
                     /locus_tag="VG272_04105"
     CDS             complement(2813..3211)
                     /gene="ndhC"
                     /locus_tag="VG272_04105"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWF12896.1"
                     /translation="MTGNDIQSYAPLLVHLILAMVLSGALLTLSSVVGWRRPSKVKMQ
                     AYECGMTPTGDAREPFSVKFYLIGIIFILFDVEAIFLYPWAFIFKSLRWFGFVEMLLY
                     IGILLVGYFYLWKKGALDWNRPAARQDRNS"
     gene            3340..3969
                     /locus_tag="VG272_04110"
     CDS             3340..3969
                     /locus_tag="VG272_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12897.1"
                     /translation="MNFGFNSNVRVGEALFHVQTEDRGPSHPYLDTVVYLSGRVIHKR
                     STGYQQFADGVDKNMLAEKLHERLAMQHREVIAQLEAGIIPIPGKSTPAVETTKAEQG
                     LELRLLNPKSWLTAGVVTLEISLEEKLSKQKINDAEVQVVLEHEKHRTECAETRTDSK
                     GFATLKFPMPVEVATGASLVVRATDGYLFGELRFRLKSKPREKTPVTVA"
     gene            3969..4181
                     /gene="thiS"
                     /locus_tag="VG272_04115"
     CDS             3969..4181
                     /gene="thiS"
                     /locus_tag="VG272_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HWF12898.1"
                     /translation="MSSNIQIVVNGESREVPQGSGISALLTLLGLPNDRVAIERNLEI
                     LPRSLWSTITVQQGDKYEIVHLVGGG"
     gene            complement(4203..4619)
                     /locus_tag="VG272_04120"
     CDS             complement(4203..4619)
                     /locus_tag="VG272_04120"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWF12899.1"
                     /translation="MTPTIQYSATFDKATPAMLYDLFADSAKHTAATGMPAKISRKVG
                     GTWTAFGKMLLGKNLVLIPNRMIVQTWRSSEWKKSDPDSILVVSFEKVAGGAKADLVH
                     VGVPEYDHEGVTQGWIKYYWEPWKAYLSSKGAKKRS"
     gene            complement(4651..5301)
                     /gene="ribA"
                     /locus_tag="VG272_04125"
     CDS             complement(4651..5301)
                     /gene="ribA"
                     /locus_tag="VG272_04125"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HWF12900.1"
                     /translation="MPRKKTKRAPAARKAAKTSKDEIRIVARAKLPTRFGPFEILGIE
                     GKTEQESAVALVRGKIRGEVPLVRIHSQCFTGDTLASERCDCRAQLELSLRKIADAPS
                     GVLLYLPQEGRGIGLMNKLRAYELQDRGMDTVEANEELGFAADSRDYGFSAEALRLLG
                     VRRVRLLSNNPDKVRQLEKSGIKVVERVPCRPRTTRTSRAYMETKRTKLGHLLDRL"
     gene            5392..>6455
                     /locus_tag="VG272_04130"
     CDS             5392..>6455
                     /locus_tag="VG272_04130"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWF12901.1"
                     /translation="MKTLTFGGDLLLTLLLHVGAAASICAWLARSATFRKVLFTELRD
                     SDQKLKLMLFVTPPLAFGVFLRIVGQPYRAADLMLEGSFLLGLLGGRVVGPLGGSIIS
                     LPAFAHHEWLSMPVAATAGLVGGLIRQTLPNTEEIWRLGPYTFLSTPRWLVRVIRRKK
                     VTWEMLPLGSIIVLELARQALGHATKIRWLFYLDAGKSWGLALVLLSTVMAVAVPMKI
                     WSNTRLEMNLEQHQQLLLKARMDALTAQINPHFLFNTLNTVSSLIRYDPDTARGVVIK
                     LSNILRRLLRKHETFVPLQEELDFIDDYLDIEIARFGRDNLVVHKEISEETLEAFVPS
                     MLLQPIVENALKHGLAAKLGG"
BASE COUNT         1465 a         1683 c         1807 g         1500 t
ORIGIN      
        1 gggaacggag gacgaattcg cggatagccg cgcttgcctt cgagcgactc cataagcgcg
       61 gattcttcgc cgcactcgta ggcgcccgcg cccgtatgcg cgaccagatc gaaatcgtac
      121 ccgctgccca gaatattttt gccgaggtag ccgcctttgt aggcttcggc aattgcttct
      181 tccatgatgt ccagaacgta gcgatactcg ccgcgaatgt agacgtatcc ctgatgcgag
      241 ttcaccgcgc gtccggcaat cacgatgcct tcgattaatt gatgcggcat caattccatc
      301 agcgggcggt ctttgcacgt gcccggctcg ctctcatccg cgttgcacaa aatgtatttc
      361 ggtttcggcg aatccttggg cacgaaactc cacttcatgc cggtagggaa tccagcgccg
      421 ccgcgtccgc gcaggtttga tttttttact tcatcgacta cctgttcggg agttagttcc
      481 tttagcgcgc gctccattcc cttgtagccg tcattcgcca cgtaaacttc gagcttgtgc
      541 gaatccttta cgccaaacag gcggctgatg gcgggcactt ccgcgggatg ccgttgtttc
      601 accgcgccgg ttatcggcgt ggaagtttgc ggcttgcggc ccgcttcgag agcttcaaag
      661 atttgcgtgg tgcgctcaac ggtcaggttc tcgtagaaat cgtaattgac ctgcatggcg
      721 ggagccccgg tacaggcgcc gaggcattcc acttcctcga gcgaaaatac gccgtcggcc
      781 gtggtttcct tgtggccaat ttccagcttt ttctgaacaa aatcgtatat ttgattgccg
      841 ccgcgcagca tgcaggaaac gttggtgcac acctgaacgt gatattttcc ggcgcgcttg
      901 cggcgcagca tggagtaata ctccattgtt tcggtgactt gcagcgtatt cagtccaagc
      961 cgctgcgcaa tttccaggat catttcatcg ctgacaaaac cgaactggtc ctgcgcgtac
     1021 agcagcatgg gaatgagcgc cgaacgcttc accggatagc ggttctccag cgtttggaat
     1081 ttggcttcca gctgcggagg caaattcatc agcggtcaat ctccccaaga atgatatcca
     1141 gggttccgat gctggcaatc gcgtcggcta ccaggtgtcc ttcgaccatc ttttgcagcg
     1201 cctgcaaatt cacaaaggat ggcgcgcgca tgcgcatgcg gtacggcttt ggagaaccat
     1261 cgctggcaat aaagcaaccg agttcgccgc gcggcgattc aatgcgctgg tagacttcgc
     1321 cgggcggagg cgcgaagcct tccgtaaaaa ttttgaagtg atagatcagc gcttccattt
     1381 ccgttttcat tttttcgcgc tgtgggggaa caattccggg cgcgtcggcc tttaccgggc
     1441 cttctggcgg aatcttttcg agggcctggc gcacaatctt caaactttcg cgcatttcgc
     1501 ccacacgaca caggtagcgc gcatagcaat cgccttcggt gcgcaccggc acattaaaat
     1561 caaattcatc gtagctggaa tacgggaaat atttgcgaac gtcataatcg acaccggaac
     1621 cgcgaagtga tggccccgaa acaccgagcg cgatggcgtc ttctgctttc aagataccca
     1681 cgccctgcgt gcgcccaatc cagatgcgat tcttggtcag caattcctca tattcgtcaa
     1741 agcgggaagg gaaagtgcga acaattttct cgacgtgatc ggtccagccc acaggcggct
     1801 caagcgcgag cccgccgatg cggaaatagc tagtcatcat gcgctggccg ctgaccagct
     1861 caaaaatttt gagaagttcc tcgcgctcgc ggaagcaata cagaaagacg gacatcgcgc
     1921 ccaggtcgat ggcatgcgta ccgagccaga cgaggtgcga gccaatgcgt gtaagttccg
     1981 ttagcagcac gcgaatgtat tgcgcgcgct tggggacctc caggccaagc agtttctcaa
     2041 cggccaggca atacgcaaga ttattggata acggcgcaag gtaatccatg cggtccgtga
     2101 tcgtgatggc ctgcgaatac gtaagcgcct cggcggtttt ttcgatgccg gtatgcagat
     2161 acccgatatc gacgtccgct ttgacgatat tttcgccatc caattccaag agtacgcgca
     2221 aaacgccatg cgtggacgga tgttgcggtc ccatgttaag gaccatggtt ttttcgggcc
     2281 cgacagctgt ttgaatggtg gtcatgtcgc aggttcgctc aggtcgaaat cagagattag
     2341 cgagggccct ccaccggata gtccttgcgg agtgggtggc cttcccaatc gtcaggaaga
     2401 agcatgcggt gcagattggg atgtccgtca aatcgaattc caaaaagatc aaatatctcg
     2461 cgctcgtgcc aatttgcggt tggccaaacg gaaacgaccg attcgacgag cggatcttcg
     2521 cccggcaggc gcaagcgcaa gcggactgtg tctcgccggg agagggaaag caaatgataa
     2581 ttcagttcaa agcgcggttc gatcggaaac cggtccacgc cagtcacatc ggaaagatag
     2641 gtgaatgcca aaaacgaatc cgatcttagg aattcacaaa ggggacgaat atgctcgcgg
     2701 taaataacaa tggtcagttc atcacggaac gaaatcagtt ctgctatagc ttcaggccgc
     2761 caatcgcgga ttttagctac gataggattt tgctcgaggt caggcgtgtc cgtcacgaat
     2821 tacgatcctg gcgtgcggct gggcggttcc agtccaacgc ccctttcttc cagagataga
     2881 aataaccgac cagcaggata ccgatgtaga ggagcatttc cacaaaacca aaccaccgca
     2941 aggacttaaa tatgaaggcc catggataca ggaagattgc ttccacgtca aaaagaataa
     3001 agataatgcc aatgagataa aattttacgg aaaagggttc acgcgcatcg cccgtgggcg
     3061 tcatgccgca ttcataagct tgcatcttta ctttggaggg acggcgccag cctacgactg
     3121 aggaaagcgt aagaagcgca ccggaaagca ccattgccag gatgaggtgc acaagtagtg
     3181 gcgcatagga ttggatatcg tttcctgtca tctgagcaca agctataata gcgatgtagc
     3241 ggcaagtggt caaatccact tgaaagatat atcgatcatt ttttcgattt agttaagaga
     3301 aatatatggt acacgtgtgt ggagaaggct gaggagcaga tgaatttcgg attcaactca
     3361 aacgttcgcg ttggcgaggc cttgtttcac gtgcaaacgg aagaccgcgg gccttcgcat
     3421 ccttatctcg atacggttgt ttatttgtcc ggacgcgtga ttcacaagcg cagcaccggt
     3481 taccagcaat ttgccgacgg agttgataaa aacatgctgg cggaaaaact tcacgagcgc
     3541 ctggcgatgc agcatcgcga agtgattgcg caactcgaag ctggaattat tccaattccc
     3601 ggcaaatcca ctccagcggt ggaaactacg aaagcggaac aaggattgga actccgtctg
     3661 ttgaatccga agtcgtggtt gacggctgga gtcgtcactc tggaaatttc gttggaagaa
     3721 aaattatcaa aacaaaaaat taacgatgcc gaggttcagg ttgttttgga acatgaaaaa
     3781 catcgcacgg agtgcgcgga aacgcgcacg gattccaaag gattcgcaac gctgaagttt
     3841 cccatgccgg tcgaagtagc caccggcgca tcgctcgtgg tgcgcgcgac agatggttac
     3901 ctgtttggcg agctgcgttt ccgcctgaaa tcgaagccgc gagagaaaac tccggttaca
     3961 gtggcgtaat gagttcaaat attcaaattg tagtgaatgg ggaatcacgg gaagttcctc
     4021 aggggtccgg gatctccgct ttactcactc tcttgggatt gcctaacgac cgcgtggcca
     4081 ttgagcgtaa tctggaaatt cttccgcgat cgctgtggtc cacgatcacg gttcagcaag
     4141 gagacaaata cgaaatcgtc caccttgtgg gtggaggctg atttttaagt ttgtagaatg
     4201 agtcaggaac gcttctttgc gccttttgaa gagagatacg ctttccacgg ttcccagtaa
     4261 tattttatcc agccttgggt aacgccttcg tgatcgtact ccggaacgcc cacatggacg
     4321 aggtccgctt ttgcgccacc cgccactttt tcaaaactga cgaccagaat ggaatcggga
     4381 tcggattttt tccattccga tgaacgccag gtttgcacga tcattcgatt gggaatcaac
     4441 acaagatttt ttccgagaag catcttgcca aacgcggtcc atgttccgcc aaccttgcgg
     4501 ctgattttgg cgggcattcc ggtcgccgcc gtgtgttttg cggaatcggc gaaaaggtca
     4561 tacagcatcg cgggagtcgc tttatcaaaa gtggcgctgt attgaatcgt aggcgtcatg
     4621 gaagattcct ccaaaggatt taaagcctga ttacagacga tcaagcagat ggccgagttt
     4681 ggttctcttg gtttccatat aagcacgcga agtgcgggtc gtccgcgggc ggcacggcac
     4741 gcgctcgact accttgattc cggatttttc gagttgccgc actttgtcag gattattgga
     4801 gagtaatcgc acgcggcgca cgccgagcaa acgcagggct tcggcggaga atccgtaatc
     4861 gcgcgaatcg gcggcgaagc cgagctcttc attggcttcc acggtgtcca tgccgcggtc
     4921 ctgcaattcg taggcgcgaa gcttgttcat caggccgatg ccgcggccct cctgcggcaa
     4981 atagagcaga acgccggacg gcgcatcggc aattttgcgt agggaaagtt ccagttgcgc
     5041 gcggcaatcg cagcgctcgg aggcaagcgt gtcccccgta aagcattgcg agtgaatgcg
     5101 cacgagtgga acttcaccgc gaatttttcc gcgtacgagc gcgacagcgc tttcctgctc
     5161 cgtttttcct tcgatgccga gaatttcaaa tggcccaaat cgcgtgggca actttgcgcg
     5221 tgcgacaatt cggatttcat ctttcgatgt tttcgcggct ttgcgcgcgg cgggcgcgcg
     5281 ttttgttttt ttacggggca taagggcctc gtattatatc aggcgatcgg cgcaagcggc
     5341 gctttacatc gacacgtcga ttggctaacg tagccgcagc ggccaaggcc tatgaaaacg
     5401 cttacatttg gcggagattt gctgctcacg ctgctgctgc acgtgggcgc ggcggcgtcg
     5461 atctgcgcat ggctcgcgcg ctcagccaca tttcgcaaag tgctcttcac cgagcttcgc
     5521 gactccgacc aaaaattaaa attgatgctg ttcgtcacgc ctccgctcgc gttcggcgta
     5581 tttttgcgaa tcgtagggca accgtatcgc gcggcggacc tcatgctgga aggttctttt
     5641 ctgttaggtt tgctgggagg ccgggtcgtc gggccgctcg gcggctcgat tataagtctc
     5701 cctgcctttg cacatcatga gtggctgtcc atgccggtgg cggcaacggc ggggcttgtt
     5761 ggcggtttga tccgccagac gcttccgaac accgaggaaa tttggaggct gggtccgtat
     5821 acttttctga gcacgccgcg gtggctcgtg cgtgtcatac gcaggaaaaa agttacgtgg
     5881 gaaatgctgc cgctcggctc gattatcgtt ctcgaactgg cgcgtcaagc gctggggcac
     5941 gccacaaaaa ttagatggct cttctacctt gatgcaggaa aaagctgggg gctcgctctg
     6001 gttttgttgt ccacggtgat ggccgtcgcg gttcccatga agatttggag caacactcga
     6061 ctggaaatga atctggaaca gcatcagcaa ctcttgctga aggcccgcat ggacgcgctg
     6121 accgcgcaaa tcaatccgca ctttctattt aacacgctga atacggtgtc ctcgctgatc
     6181 cgctacgatc cggacacggc gcggggagtg gtcatcaagc tgagcaatat tttgcgacgg
     6241 ctgctgcgca agcacgaaac gtttgtgcct ttgcaggaag agctcgattt cattgacgat
     6301 tatctggata tcgaaattgc gcgctttgga cgcgacaatt tggtggtgca caaagagatc
     6361 agcgaggaaa cgctggaagc tttcgttccg agcatgctgc tgcagccgat tgtagaaaat
     6421 gcgctgaagc acgggctggc cgctaaactg ggcgg
//
LOCUS       DASZMB010000089        12321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3646349, whole genome shotgun sequence.
ACCESSION   DASZMB010000089 DASZMB010000000
VERSION     DASZMB010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 12321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12321
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3646349"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VG272_04135"
     CDS             complement(<1..653)
                     /locus_tag="VG272_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HWF12902.1"
                     /translation="MNNFTFTRAIDTDSALKLVSASPDNTAARFLAGGTNLIDLMREG
                     IEQPQSLVDITPLPLSEVRELPDGGLRVGAMVRNSHLAAHPLIRERYPLLSQAILMGA
                     SAQLRNMATTGGNLMQRTRCYYFYDNAAACNKRVPGSGCDALDGFNRIHAILGASSRC
                     IATHPSDMCVALAALDAIVNITGPSGERQITAAEFHRLPGETPEIDTNLKSGELITSV
                     "
     gene            complement(650..1165)
                     /locus_tag="VG272_04140"
     CDS             complement(650..1165)
                     /locus_tag="VG272_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWF12903.1"
                     /translation="MNASISLHVNGISRILNIEPRVSLLDALRDHLGLTGTKKGCAQG
                     ACGSCTVLVDGERINSCLALAVQYEGREIVTIEGLADSGVLHPLQQAFIEHDGFQCGY
                     CTPGQICSAVGMLSEFRSGAPSAVTEDGASTNELTDAELGERMSGNLCRCGAYVGMRE
                     AIQQVYAMGKP"
     gene            complement(1494..2873)
                     /locus_tag="VG272_04145"
     CDS             complement(1494..2873)
                     /locus_tag="VG272_04145"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWF12904.1"
                     /translation="MDSLTARSATFSLLVVDDELTTRDLCRDVAGDAGFKVYVAPTTE
                     DAISILDQYPVDIVVTDLKVPQIGGLELLKRVRANNPETAVVVLTQYGTIATAVEATR
                     LGAADYVTKPFHVDELRAKLDRLTHTIEMGQENRLLREELRSRPGFGGLVGVSPRMER
                     VYKLIQKVAMHTYPVLILGESGTGKELVARSIHFSGPRKNKAFAPVDCSSLTPTLIES
                     ELFGHVRGAFTGAQHSKQGLLEAAGDGTLFLDEIGELPIDLQSKLLRALQEHEIKPVG
                     SNERVGIRARIIAATNRDLESAIRTGAFRQDLYFRLNVVQIKLPPLRERKADIPLLVN
                     TFLDKFSDPARPIHTISEDAMRRIMSYDWPGNIRELENAIERAVALGSGPILHVGDLP
                     SNLQYSSSEKLADVDELMPLETLERRAIFRALQETSGDKLAAARLLGIGKTTLYRKLK
                     QYESGRVQV"
     gene            complement(3199..5193)
                     /locus_tag="VG272_04150"
     CDS             complement(3199..5193)
                     /locus_tag="VG272_04150"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HWF12905.1"
                     /translation="MRDQNLNLTMRKFVCTFLFLPLFNLAHAQTWKPLGPPGGDVRVL
                     ATDPSHLERIFLGTADGHIFGSEDSGKHWTLLGRASARSDAVITSIVVDPRNGNKLFA
                     SSWTRDSATEGGVYRSLDGGHTWTVAGLAGEAVRVLAMAPSDSNVLVAGTLDGVYRSN
                     DASKSWQRISPKNHAELRNLDSIALDPHDSQIIYVGTFHLPWKTQDGGRTWSPIHQGM
                     IDDSDVMSLLIDSEISGRIYASACSGIYRTDDSAAGWRKIQGIPYTARRTYAITQDPN
                     HPDYVYAATSEGLWKTEDSGMTWHRTTPDSWVINAVVVSKGDPGRVLIGTEERGVLVS
                     DDAGEHFEDANVGFEHRQIVAVRADRNRPGRILAVIADAPDSVQSSDDGGRSWSPLGT
                     GLRSEQVLRIFPAPDNSWWISLSSGGLMRYDEAKKNWTKIGILMDKSTMTLAPNSKLK
                     PRPVATKRTDPPLLDVVSDIDFHSNNWYAATSVGLLVSVDHGLTWKVKQAGPLVSLAL
                     QSVRVSSDERRIRVVSQRGMLYSDDAGNSWTWHDLPLESGGAIALVDQPGHDNTLVAT
                     AHNGLYISRDWGKTWDAAGSGLPSAAVQDFVATEATFVASMRTGGLFLSSDSGKTWSR
                     ASGNFADEFFTAVAASEISDRVFAASSTEGLYEVLWTATH"
     gene            5587..6141
                     /locus_tag="VG272_04155"
     CDS             5587..6141
                     /locus_tag="VG272_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12906.1"
                     /translation="MRLSKKSWLVLSAFFMAFVFVPASHAQLNSGASTVNLNAPLAES
                     LTVSAGPATVNLALPPSGTANGSAPVSITTNWALAKTRTSVKLYAYFSTGNAMTDGAG
                     DNIPVANVSGSVNGGAAAAFTGATPFGGSTGMTVFTQAISPTTGPFNSSHGPDTIALT
                     ITTTGLSLPAATYTGVLNIQAQAI"
     gene            6396..6923
                     /locus_tag="VG272_04160"
     CDS             6396..6923
                     /locus_tag="VG272_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12907.1"
                     /translation="MIFCLPILILLLVGTPAHAQLNSNIAGVNLAANVTTSLTVSASP
                     GLVNFVLIPSGISNGSTAITVNTSWTLSPSVGTVKTYAYFTSAAAALTDGAGDNIPSS
                     SVSGSVDGGAFGAFTGASPFAAASSVTVSTIRILGNNKNGNQSDTLNMRISTVGLTLP
                     AANYTGVLTIQAQAL"
     gene            7001..7705
                     /locus_tag="VG272_04165"
     CDS             7001..7705
                     /locus_tag="VG272_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12908.1"
                     /translation="MKRHWALLLLLLCATARTPAQTISPVIQEYQGKADGKFQIYNDA
                     DVPLTVVLEPHSFGVDSNGNPTFRKLDPEIRVQLSSTSFRIQPKQTYFVFYKASSEVL
                     PNWFCIYATVTGTTTSTGIKLALQLPHTVYMLDKNSLNPDQIAWIRAESSNEGNKRQI
                     VAEVENHSKTFSRVRDVQVTSANGKQSFAGFPLFPGQRRLIELDWDGSSTPEHIQLNF
                     DHFKKESDLRAASSGQ"
     gene            7702..9423
                     /locus_tag="VG272_04170"
     CDS             7702..9423
                     /locus_tag="VG272_04170"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWF12909.1"
                     /translation="MNRAFPPRCGTRGLAKWLIRFALVCLVSMAVGRTTRGQAFSLTG
                     GSSSMFDAHGASVEMRSPFYTGRLDFGYMNGPSLGFSLLHLYRGDLLDAGDQEIPLVF
                     PTDLFNRSYYFFGRGLSLLHKTKNTRLFLFTGVTSTNYWSPFLNVARGGSFTAAIFYE
                     KQISPSVRWFSSNVYATRETSIQSVEWTARKDIKMALSAGVGNNQAYSASSFSWSTPV
                     FSVDASYALTGNAFKQVEAYTPDLTENDRENVRVEYAPFKAVRIVASRNNYLAPVESG
                     ITSKAAVNGFGIWTGCTGSQFYGSLYQSSTSYGKSIAYAFGAQRDITRRLQVGTNYLG
                     TRSSGHESHTILGNVRETFSSRLSLNQVINHSSGQTGVSFGGDFISNFMTLSVDYQTV
                     FLPFVQAGSGQLKQVIVIGLHFQLPRGIQFNAGTNVTPLGQIRYTTYGSTYAYHGLAP
                     SSPGASFSGAFFQNAVHGQVLNPDGQPIEGAALRIGTETAFTDSDGNFIVRVKKAGDL
                     NLKIAFDEFTTPGNYVVVQAPATVKAVRDDSAEMYKIVLRRIPNSFSASDPQHTPANP
                     DRTSNLE"
     gene            complement(9576..11660)
                     /locus_tag="VG272_04175"
     CDS             complement(9576..11660)
                     /locus_tag="VG272_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWF12910.1"
                     /translation="MLRFAARCGGLLTLIFMLITPAFSKTLLADNDAKNSEDAVSANS
                     ADTTPAPAPQPPADATASAQQTTGSEKKDTSNEYKPAPKFTPMLATTGTLGLFTLETA
                     DTLPKHGFAFSAFGNKFGRMPGSVTLFQVGIDASYGITDNLNVYATFDPYGHVHVGCP
                     GQLSLRSIPLAGNCAPVAFGSSAPGSFFPVVAGSAPGYVEDYPFAANNTGGIGNITLG
                     VKYALMNQQQGARVGLSVRNDLIISMKTDVNKLLANGTQGSPLSDLVSIAVSRQWGNI
                     LTGTFNFGYMFVRAPRDNNDNHLFDMANQLKTGAGILLFPESRFQPMMEYSAIVLDSA
                     PNSPDNTTLGARDPIDGIWGLRMYLKKNIGVDLGYRYMLNLRNSSDRSGFVVKIGTAY
                     WPEKAPPVNHPPTITCAADKPMVYADSNDAVAISCTASDPDNDPLTYTWSSTSGKVDG
                     SGPQVRWLSNGVSVGTSTITAKVDDGRGGSAASTVSVSVETKPNHPPTITCFADRPTV
                     FAGEKVHIKCNASDPDGDPLSYTWRANSGNLVGNGPAADFDTTGLAPASYPITTRVED
                     GRGGAADAMTSVDVKAVPPPPQASKISDCAFGQPGSTRIDNVCKRILDDVALRLQNEP
                     KGSAVIIGYSDPKERKPEKVSGDRATNAVKYLGEKGIDASRVTTKTGSGEAGAKDNRR
                     IDVIWVPEGATY"
     gene            12046..>12321
                     /locus_tag="VG272_04180"
     CDS             12046..>12321
                     /locus_tag="VG272_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12911.1"
                     /translation="MKKTFRAPAVVVIFLLSAIGICITAASAGQTRSATSGSWADSVR
                     NAPHFLVRLDQELNTGKNRNHEKFQVQTIEPLVASNGHIFPVGTKILG"
BASE COUNT         2891 a         3322 c         3246 g         2862 t
ORIGIN      
        1 accgaggtga tcagctcgcc cgatttcagg ttcgtatcga tctcgggtgt ttcccccggc
       61 aggcggtgaa actcggcggc tgtgatttga cgttccccag acggtcccgt gatattcacg
      121 atggcatcga gagcggcaag cgcgacacac atgtcggaag gatgagtcgc gatgcatcga
      181 gacgaagcac cgagaatcgc gtgaatccgg ttgaacccgt ccagggcatc gcaaccgctg
      241 ccggggactc gcttgttgca agcggccgcg ttgtcgtaaa aataatagca acgtgttcgc
      301 tgcatcaaat tccctccggt ggtggccatg ttgcgcagtt gcgccgatgc gcccatgaga
      361 atggcctgcg aaagcagcgg atatctttcc cggattaagg gatgtgcggc aaggtggctg
      421 ttccgtacca tcgctcccac tcgtaggccg ccgtcgggaa gctcccgtac ctcagaaagc
      481 ggaagtggag tgatatccac aagggattgc ggctgttcga tcccttcgcg cataagatca
      541 atcaaattgg tgccgcccgc aaggaagcgt gccgcagtgt tatccgggct tgcagaaaca
      601 agtttaagag cggaatctgt atcgatcgcc cgagtgaaag taaagttgtt catggtttcc
      661 ccattgcata gacctgctgg atcgcttcac gcatgccaac gtaagcgccg caccggcaca
      721 gattgccact catgcgctcc cctaattccg cgtccgtcaa ctcgttggtt gaagcgccat
      781 cttcggttac tgcgctgggc gctccgctgc gaaactcact cagcatgccc acggcggaac
      841 agatttggcc tggtgtgcaa tacccacatt gaaagccgtc atgctcgatg aatgcttgct
      901 gcagcgggtg caacaccccc gaatccgcta acccttctat cgtgacgatc tcccgccctt
      961 cgtactgcac ggccagcgca aggcaggaat taatccgctc gccatctacc aggaccgtgc
     1021 aagacccaca cgcgccctgc gcacaacctt tcttagttcc agtgagtccc aaatggtccc
     1081 gcagggcatc aagtaatgac acgcgcggct caatgtttaa aattcggcta attccgttga
     1141 catgaaggga aatactcgca ttcattcgtt cacctcaatt acaaacccat gcgcatgacc
     1201 taaggccagg ctttagaaca acccaggtgc ctagcgtcaa tatcgctggg atattttgaa
     1261 aaacaatacg gtttttccag gaggaaaata cccgggattg gatgagtaga aagatttaca
     1321 aaaagcagca cgcaaactca atatctacgt ttttacttct caggcacgat tgcccagagg
     1381 gccgttaccg ccaatagatt agagccgcat tcgttcaatt tgccctgccc aaatgaggat
     1441 gttccaccct ccactcgcta gcaactaaac atttcagatg cgggaaataa aacttacacc
     1501 tgcacgcgcc ccgattcgta ttgtttcagc ttgcggtaca gcgtggtttt tcctatgcca
     1561 agcaggcgcg cggcggccag cttgtctccg gaggtttcct gcaaggcgcg gaagatggcg
     1621 cggcgttcca gagtttcgag cggcataagt tcatcgacgt ccgcgagttt ttcggatgag
     1681 gagtattgca agttcgatgg taaatcgccc acgtgcagga tcgggccgga accgagggct
     1741 acggcgcgtt cgatggcatt ctccagttca cggatgttgc ccggccagtc ataagacatg
     1801 atccggcgca tggcatcttc ggagatggtg tgaatcgggc gggcgggatc ggaaaatttg
     1861 tcgaggaagg tgttgacgag aagaggaatg tccgcctttc gctcgcggag cggcggcagc
     1921 ttgatttgaa ccacgtttaa gcggaaatag agatcctggc ggaacgctcc cgtgcgaatc
     1981 gcggattcca ggtcgcggtt ggtggcggcg attatgcgag cgcgaatgcc aacacgctcg
     2041 ttcgagccaa caggcttgat ttcgtgttcc tgcagcgcac gaagcaattt cgattgcaga
     2101 tcgatgggca gctcgccgat ctcatcgaga aatagcgtgc cgtcgcccgc ggcttcgagc
     2161 agaccttgct tggagtgctg cgcgccagtg aatgctccac ggacgtggcc gaacaattcc
     2221 gactcgatca gcgtaggagt gagcgaagag cagtccacag gggcaaatgc tttatttttg
     2281 cgcggccccg aaaagtgaat cgatcgcgcc actaattctt ttcccgttcc gctctcgcca
     2341 agaatcaata ccggatacgt gtgcatggca actttctgga tcagtttgta aacacgctcc
     2401 atgcgcggcg aaacaccgac caggccgcca aatccggggc ggctacgcaa ctcctcgcgc
     2461 agcaggcgat tttcctgccc catttcgatg gtgtgagtca ggcggtcgag cttggcgcgc
     2521 aattcatcca catggaacgg cttggtcacg tagtccgccg cgccgagacg cgtggcttcc
     2581 acagctgtcg caattgttcc gtattgcgtg aggacaacga ccgcggtctc gggattgttc
     2641 gcgcgtactc gtttcaggag ttcgagtccg ccgatttgcg gcaccttcaa atccgtaaca
     2701 acaatgtcca ctggatattg atcgaggatc gagatggcgt cctcggtcgt tggtgcgaca
     2761 tagactttga acccggcatc accggcaacg tcccggcata aatctctcgt agtgagttca
     2821 tcgtcgacaa ccaggagact gaaggtcgcg ctgcgcgcgg taagtgagtc cactctcgtt
     2881 ccggtttcca tgtggaacct cttttcacaa tggagcaaca atcgtgccaa atcaggagaa
     2941 agacctaagc cagtccgact ctgtcactta tcgtgacacg ttccttaatg tgctggagat
     3001 tctattgccg aattgggaag taaagcaagc ttaattctgc gccatggcga gtaattcctt
     3061 tggtatgcag gtgaagcaaa aatgggaatc ttgcaagtat caaatgggca cgactcttga
     3121 cacttccaga tatttagcga acttactttt ttaatctgta ggtttgttta ctaaaacagt
     3181 gacatcaccc gaaagtattt agtgcgtagc agtccacaga acttcataaa gtccttcggt
     3241 cgaagaagcc gcaaaaaccc tgtcagagat ttcggatgcg gccactgcgg taaagaactc
     3301 gtccgcaaag ttaccgcttg cacggctcca tgtcttgcca gagtcggaag atagaaacag
     3361 acctccagtc cgcatggagg ctacgaacgt tgcctcggta gcaacgaaat cctgaacggc
     3421 cgcggagggc agacctgaac ccgcagcgtc ccacgttttt ccccagtcac gagaaatgta
     3481 aaggccgtta tgggcagttg cgaccagcgt gttgtcgtgg ccaggttgat cgactaaagc
     3541 aatagcgccg ccggattcca gcggtaaatc atgccacgtc caagaattcc cggcatcgtc
     3601 ggaatacaac attccgcgct gcgacacaac gcggatgcgt cgttcatccg aagaaacacg
     3661 aacggactga agcgcgaggg agaccagcgg cccggcttgt tttaccttcc acgtgagacc
     3721 gtgatcgacg gaaacgagca agccaacgct cgtagcggcg taccaattgt tcgaatgaaa
     3781 atcgatatcc gaaaccacat cgagaagcgg cgggtcagtt cgttttgtgg cgaccggcct
     3841 gggcttaagt ttcgaatttg gagcaagagt catcgtcgat ttgtccatca aaatcccaat
     3901 tttcgtccaa tttttcttcg cctcgtcgta tcgcatgagc ccgccactcg aaagtgaaat
     3961 ccaccaggaa ttgtctggag cggggaaaat gcgcaggact tgttcggagc ggagtccagt
     4021 gccaagtggc gaccaggacc ggccgccgtc gtccgatgac tgaacggagt caggagcgtc
     4081 ggcgatcacg gcgagaattc gaccgggccg atttcgatcc gcgcgcactg cgacaatttg
     4141 cctgtgttcg aaacctacgt tggcgtcctc gaaatgctcg cccgcgtcgt cactcacgag
     4201 aactccccgt tcttcggtgc cgatcagcac tcgtcccgga tctcctttgg agaccactac
     4261 agcgttaatc acccaagagt cgggagttgt gcgatgccag gtcattccgc tgtcctccgt
     4321 cttccacaag ccttccgagg tggccgcgta cacataatct ggatgattcg ggtcctgcgt
     4381 aatggcatat gtgcggcgcg cggtatatgg aatcccctgg atctttcgcc acccggccgc
     4441 actgtcatcg gtacgataga tgcccgaaca ggcgctggcg taaattcgcc cggaaatttc
     4501 ggaatcgatc agcaagctca tgacgtcgga atcgtcaatc atgccctgat ggataggact
     4561 ccaggtgcgc ccgccgtcct gcgttttcca cggtagatga aatgttccca cataaataat
     4621 ttgcgaatcg tgcgggtcca gggcaatcga gtcgagattg cgcaactcgg cgtggttttt
     4681 gggagaaatg cgctgccatg atttcgaggc gtcattcgaa cgatatacac cgtccaaggt
     4741 tcctgccacg agcacgttcg aatccgaggg cgccatcgcg agcacgcgca cggcttcacc
     4801 ggcgaggcct gcgacagtcc atgtatggcc gccgtcaagg ctgcgataca ctcctccttc
     4861 tgttgcggaa tctcttgtcc aggatgacgc gaacaattta tttccatttc gcgggtcgac
     4921 tacgatggaa gtgatcacgg catcggagcg cgcgctggcg cgcccgagga gagtccaatg
     4981 cttgccggaa tcttccgaac caaaaatgtg gccatctgcg gtgccgagaa aaatccgttc
     5041 gaggtgcgaa ggatccgtag cgaggacacg cacatcgcct ccgggtggcc ccagcggctt
     5101 ccaggtctgg gcatgggcca aattaaataa gggcaaaaat aagaacgtgc aaacaaattt
     5161 acgcatggtg agattcaaat tttggtcccg catctttgct cgagctctat tgtataagcg
     5221 attcagaaat aacctgttac cgaaaattac ctaataaggt ctgtaaatgg gcgcactcct
     5281 gtttttgaac attattttct tccaggatgt aacttcattc gcttaataga gcagaaaaat
     5341 gacgcgcagc acgcctgtga aatcttactt agatgtttca tcctgaaatt gtgagtccgt
     5401 aattgtttca ttttgggtat ttactctcag gaataggtac cacggtacta gagccacttc
     5461 ccattttgaa acgtaaaacg gaatagctgc gggcgaatca ggccagacgg attctggaca
     5521 tcgcgccgca caggttgaaa gaactgtaat gttcctcagc ctaaaattaa acttttcgga
     5581 ggcggcatgc gtttatcgaa gaagtcctgg ctagtgctct cagcgttctt tatggcattc
     5641 gtgttcgttc ctgcttctca cgctcagctg aattctggtg cgtccacggt aaacctcaat
     5701 gcgcctctcg cggaatccct aacggtttcc gcaggacctg cgacggttaa ccttgcattg
     5761 ccgcccagcg gcacggccaa tggcagcgca ccggtatcga tcacaacgaa ctgggcgctc
     5821 gcaaagaccc gcaccagcgt taaactttat gcctacttct cgacgggaaa tgcgatgaca
     5881 gacggagccg gcgataatat ccctgtcgcc aacgtgagcg gaagcgtcaa tggtggcgcc
     5941 gcagctgcat tcaccggcgc aactcctttt ggcggctcaa cgggcatgac cgtattcacg
     6001 caggcgattt ccccgacgac cgggccgttc aattccagcc atggtccgga cacgattgct
     6061 ctgacgatca ccacgactgg cttgtcgcta cccgcggcaa cctacaccgg cgtgttgaat
     6121 attcaagcgc aggcgattta attactccaa cagttgcagc cgagccatcc gaatcggatg
     6181 gatagcgcat tgagcggcag ttcggacgcg gtgtggcgtc cgaacccctg ccctcagccc
     6241 tggttcacct ggaccgagga tatacaaaag aagtgcgcgg ctcacctcgc aattgcaatc
     6301 ttgaaaaaga aaagtacggg aaaaggaaat cagttctgat gttgctgaga gatcacaaat
     6361 cggaaggcaa cgctccgcgg atacttccgc ggggaatgat tttttgtctg ccgattctga
     6421 ttctacttct ggtgggaacg cccgctcatg cgcaactgaa ttccaatatt gcaggtgtca
     6481 atctggccgc gaatgtaact acttcgctaa ccgtcagcgc ttctcccggc ctcgtcaatt
     6541 tcgtactgat cccaagtgga atctcaaatg gcagcacggc cattaccgta aataccagtt
     6601 ggacgctgag tcccagcgta ggtacggtta agacgtacgc ctactttaca agcgccgcgg
     6661 cggccctgac cgatggcgcg ggcgacaata ttccatcgtc cagcgtatcc ggaagcgtcg
     6721 acggcggtgc gtttggagca ttcaccggcg ccagcccgtt tgcagccgcc agcagtgtca
     6781 cggtttccac gataagaatt ctgggcaaca acaaaaacgg aaatcaatcg gacaccctaa
     6841 acatgagaat cagtactgtc ggattaactt tgccagctgc aaattatacc ggcgttctga
     6901 ccattcaagc ccaggccctg taggaaatcg cggcagcgat gcaaatcggg taatttttaa
     6961 gttttttaac tttcaaactc ggcttcacgg ggggaattcg atgaaacgcc attgggcatt
     7021 gttattgctg cttctctgcg caaccgcgcg cactcctgcg caaaccattt cacctgtgat
     7081 tcaggaatat cagggaaaag cagacggaaa gtttcaaatt tataatgatg cggatgttcc
     7141 gctcactgtc gtacttgaac cgcacagttt tggagtggac tccaacggca atcccacctt
     7201 tcggaagctc gatccagaga ttcgcgtaca actttcgtcg accagtttcc gcattcaacc
     7261 gaaacaaact tattttgttt tttacaaagc aagttcagaa gtgctgccga attggttctg
     7321 catctacgcc accgtaacgg gaacgacgac gtcgaccggg atcaagttgg cgctgcaact
     7381 tccgcatacc gtttacatgc tggacaagaa ttcattgaat ccagaccaaa ttgcctggat
     7441 tcgcgccgaa tcctcgaacg aaggcaataa gcgccaaatc gtcgccgaag ttgagaatca
     7501 cagcaaaacg ttcagccgcg tgcgcgacgt gcaagtcact tccgccaatg gcaagcagtc
     7561 tttcgccggg tttcctcttt tcccgggaca acggcgtttg attgagctcg attgggatgg
     7621 atcgtccacg ccggaacaca ttcaactgaa tttcgatcat ttcaaaaaag aatcggattt
     7681 gagggcggcc agttcaggac agtgaaccgc gctttcccac cgcgatgcgg gacacgtgga
     7741 ttggcaaaat ggctgatccg ctttgcattg gtgtgcctgg tcagtatggc cgtagggcga
     7801 acgaccagag ggcaagcctt ttccctgacg ggcggatctt cgtccatgtt cgacgcgcac
     7861 ggagcctcgg tcgaaatgcg ttcgcctttt tacacggggc gtcttgattt cggttatatg
     7921 aatggcccga gcctcggttt ttccctctta catttgtatc gcggtgactt gctcgatgcg
     7981 ggtgaccagg aaattcctct cgtatttccc acggatttgt tcaatcgctc ttattacttt
     8041 tttggacgcg gcttgagcct gctgcacaaa accaaaaata cccggctgtt cctgtttacc
     8101 ggtgtgacct cgacaaacta ctggtctccg tttctaaacg ttgcgcgggg cggttccttt
     8161 acggcggcaa ttttctatga gaaacaaatc tctccttcgg tccgttggtt ttcaagcaat
     8221 gtgtatgcaa cccgagagac atcgatccaa tccgtggaat ggaccgcgag aaaagatatc
     8281 aagatggctc tctccgcagg agtgggaaac aatcaggcat acagcgcttc cagtttttcg
     8341 tggagcacgc ctgtgttttc cgtggatgcg agctacgcgc tgacgggaaa tgctttcaag
     8401 caggtggaag catacacgcc tgacctcacg gaaaacgacc gcgaaaatgt gcgggttgaa
     8461 tatgcgcctt tcaaagcggt gcgcattgtc gcaagccgca acaactatct agctccggta
     8521 gaatcgggaa ttacttcgaa ggcggcagtg aatggttttg gaatttggac tgggtgcacc
     8581 ggcagccagt tctacggctc gctttaccaa tcctctacct cttacgggaa atccatcgct
     8641 tacgcatttg gcgcacagcg cgacatcacg aggagactcc aggtaggtac gaattatctt
     8701 ggcacgcgct cctcgggcca cgaatcgcac acgattcttg gaaacgttcg agaaacattc
     8761 agttcccggc tgagcttgaa ccaggtgatc aaccatagca gcggacaaac aggagtctca
     8821 tttggcggag attttatttc taatttcatg accttaagcg ttgactacca aaccgtcttt
     8881 ctgccgtttg tccaagctgg ttccggccag ctgaaacaag tgatcgtcat agggcttcat
     8941 ttccagttgc cgcgagggat tcaattcaac gcgggcacaa acgtaacgcc actcggccag
     9001 atccgttaca cgacttacgg ttccacttac gcatatcacg gactcgcacc gtcatccccg
     9061 ggcgcatctt tctccggcgc cttttttcaa aatgccgttc acggccaggt tcttaatccg
     9121 gacggccaac ccatcgaagg tgcggccctg cgcattggga ctgaaactgc cttcacggat
     9181 tctgacggaa actttattgt gcgcgtaaaa aaagcgggag acctgaatct gaagattgcg
     9241 ttcgatgaat ttacgactcc cggaaactac gtggttgttc aggctccggc aacggtaaaa
     9301 gccgtgcgcg atgattccgc ggaaatgtac aaaattgttt tgcggcgaat acccaattcc
     9361 ttttcagctt cagatcctca gcatactcct gcaaatcccg atcgcacttc caatctggaa
     9421 tgatgtctaa cctgcgcatt ttttttctgg aatacaccac aagggtcggc tgcaaaaatc
     9481 aatttctgaa aagaaattgg gctccgcgtt tgccgcaatc acggcgtcca cggagcccaa
     9541 atgttcagtc tgaaacgcgc attacgagaa gcacctcagt aagtcgcgcc ttccggcacc
     9601 cagataacgt caatgcggcg attatccttc gcaccggcct cgccgctgcc ggttttggtg
     9661 gttacgcgag acgcgtcaat gcccttttcg cccaaatatt tcaccgcatt tgtggcgcgg
     9721 tcgccggaaa ctttctccgg cttgcgttcc ttgggatcgg aataaccgat gatcacggcg
     9781 ctgccctttg gttcgttctg aaggcgaagc gccacatcat cgaggatgcg tttgcagacg
     9841 ttgtcaatgc gcgtgctgcc cggttggccg aacgcgcaat cgctgatctt gctggcctgc
     9901 ggcggtggag gaactgcttt gacatcgacg gatgtcatgg catccgctgc gccgccgcga
     9961 ccatcttcca cgcgtgtcgt gatggggtag ctcgccggcg caagacccgt cgtgtcgaag
    10021 tccgcagccg ggccgtttcc aaccaggttt ccgctattcg cgcgccatgt gtaggaaagc
    10081 gggtcgccat cgggatcgga cgcattgcat ttgatgtgaa ctttttcgcc ggcgaatacc
    10141 gtggggcgat ccgcgaaaca cgtaatggtg ggcggatgat ttggtttcgt ttccacgctg
    10201 acagaaacag tggaagccgc ggaaccgccg cgcccgtcat cgacttttgc cgtgattgtc
    10261 gaagtaccga cagagacgcc attcgaaagc cagcgaactt gtgggcccga accatccacc
    10321 ttgcccgaag tcgatgacca cgtgtaggtc aacgggtcat tgtccggatc gctcgcggtg
    10381 cacgagatgg ccacggcatc attcgaatcc gcgtacacca tcggtttgtc cgctgcgcat
    10441 gtgatggtcg gaggatgatt gaccggaggc gctttttcgg gccagtacgc ggtgccgatt
    10501 tttacgacga agccgctgcg atccgagctg ttcctgaggt tcagcatgta gcgatagccg
    10561 aggtcgacac caatattttt cttgagatac atgcgcagac cccaaattcc atcaatggga
    10621 tcgcgcgctc caagagttgt gttatccggt gaattaggag cgctatctaa gacgatggcg
    10681 gaatattcca tcatgggttg gaagcggctt tccgggaaaa gtaaaattcc ggcgccagtc
    10741 ttcagttggt tggccatatc gaacaaatga ttatcgttgt tgtcgcgcgg ggcccgcacg
    10801 aacatatatc cgaaattaaa tgttccggtc aggatattgc cccactgcct gctcacagcg
    10861 atcgaaacca aatcgctcaa agggctgccc tgggttccat tggcaagcag tttgttcacg
    10921 tctgtcttca tcgaaatgat caaatcgttt cggacagaca ggccgacgcg agcgccctgc
    10981 tgttggttca tcagcgcgta cttaactccc aaagtgatat ttccgatgcc gcccgtatta
    11041 tttgccgcga acggataatc ctctacatag ccaggcgcgc taccggcaac cacagggaaa
    11101 aatgagcctg gagcggaact gccaaatgca acgggagcgc agttacccgc caaaggaatt
    11161 gagcgcagac tcagctgtcc ggggcatccg acatgaacgt ggccgtaagg gtcaaacgtg
    11221 gcatagacat tcaggttgtc cgtaattcca taactcgcat cgatcccaac ttgaaataag
    11281 gtgacgcttc ccggcatgcg tccgaattta ttaccgaacg cggagaaagc aaagccgtgc
    11341 ttcggcagcg tgtcggctgt ttccaaagta aaaagtccaa gggtacctgt cgttgccagc
    11401 atgggagtaa attttggcgc gggcttatat tcattgctcg tgtctttctt ttcgcttcca
    11461 gtcgtttgtt gcgcggaggc agtcgcgtct gccggcggtt gtggcgcggg cgccggcgtt
    11521 gtgtcggcag aatttgcgga gaccgcgtcc tctgaattct tggcatcgtt atccgccagt
    11581 aaagtttttg agaatgcggg cgtaatcagc atgaaaatta aagttaataa accaccacaa
    11641 cgcgctgcga atctcagcat gaacaaaacc ccctgaaatt attttctgga tctggacatt
    11701 ctgtaaatca tcgaagtctg aaataataat ttcatctatt ttttcagcgc caagctaccg
    11761 aggacgcgga acacaacccc atatatcggc ctgccgagga ccgatattat aatcagacca
    11821 taaacggcac aatacacgat ttccttctgt tcatattcta gtaagagatt cgaagatgcc
    11881 tcattcagaa atgagcatcc ttttttaatt catagaccac cctccggact tttctgtcat
    11941 ccgatttgat gacgtgaaca ggcagacccc cgttgataaa aatgatccgt ctgcaaattc
    12001 cgtgagcgaa tggcaggaag aaacgaagat cggaggattc aagaaatgaa aaaaactttt
    12061 cgagcgcctg cggttgtggt tatttttctt ttaagcgcca ttggaatatg tataacggcc
    12121 gcatcggcgg ggcaaacccg gagcgccacg tcaggttcat gggccgattc tgtgcgcaac
    12181 gctcctcatt tcctggttcg tctcgaccag gaattaaata caggaaaaaa cagaaatcac
    12241 gaaaaatttc aggtgcaaac gattgaacct ctcgtggctt ccaatggaca catattcccg
    12301 gttggaacca aaatattggg g
//
LOCUS       DASZMB010000090         8287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4219682, whole genome shotgun sequence.
ACCESSION   DASZMB010000090 DASZMB010000000
VERSION     DASZMB010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4219682"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..1204
                     /locus_tag="VG272_04185"
     CDS             140..1204
                     /locus_tag="VG272_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWF12912.1"
                     /translation="MTQNSDYRFNNFLFYGVILCLAYLVFRVFEPFLAPLGWAAVFGV
                     LFHSLNKRFERKWGSTRSAALITLGVTLILIIPVLFLAVMFVREGIVAAQDIQRAVGN
                     GQYGWFGRAWAWIAAKIAAQGIDLDLPGLVRQGASRAGEYMAAELGQIIRNIVIFIFE
                     LFVTLFALFYFVKDGDTILARFRNFLPFEETTKERMLAEARELVFASVTTSVVIALVQ
                     GLICGSAFALVGLGSPIFWGVVMAFLSLLPVVGAWPVWIPAAIWLFSSGHWGRAVILI
                     TICGAVGGTVDNVLRPVLLGGRASLNGLLVFISVLGGIEVFGILGVILGPIVVATAVG
                     ILDVYSGMDEAERTPKVLGF"
     gene            1383..3839
                     /locus_tag="VG272_04190"
     CDS             1383..3839
                     /locus_tag="VG272_04190"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF12913.1"
                     /translation="MATEPMILPVGLPAEKLRWRCDPATIPFATTAEAEPVKELLGQE
                     RAFRALKMGVEVTAPGFNIFVCGLAGTSRGGTITHLIDQIHPPSKASLDRCYVNNFKS
                     PDRPRLIALPRGQANNFKKDVQSGIEFLRRRIPQVFEGEPFQRQKGRIVDRFTVREKE
                     LMDDFTHRIAREQFALGHMQVGAVALPEIFPVLEGQMVPIEDIPKMVHEGKLETIVAE
                     DLERKYEQFRQEFTVVYIKTLTLSRELASELSYLEQEAASVLVDGFIEELKEKYLGTT
                     VSEYLEEVRHSILDNLDPFKEREGEEDPGGGPPPESGQKPPTNERDPFRVYGVNVVLA
                     HTDEDKSSVIFETTPTYTNLFGTIQRSYDSRGGWNSDFMDLRPGSILRADGGFLVMYA
                     LDALTEAGVWRTLKRMLNHAKLEIQPLEMFFPFGSSALKPEPIDIDVKLILIGDRHMY
                     ELLYNFEEDFRKIFKVRVEFDEEMVMSDAVIRHYGDRLRQLSEQEKIGPFDRTAVASM
                     LEYGVRLAGRRNKITARFPELADLAREAAYIARQDSSDVVNGAHVRRAREARIERHNL
                     TETKIREMIEEGTLMIETDSARVGQINGLSVLEIGGYAFGKPVRITASASLGKTGLIN
                     VEREANLSGSFHDKGMHIIAGYLRMKFAQDKPLSLAASICFEQSYSGVDGDSASSTEI
                     YALLSALSGLPIRQDIAVTGSVSQQGDIQPIGGVNQKIEGFFDVCRVKTGLTGRQGVM
                     IPYENIEDLMLRDDVIEAVAAGKFHVLPVKTLEEGIEILTGVKAGRRDAGGVFEAGTV
                     YAQVDRRLHEMATTMKDFQS"
     gene            complement(3972..4832)
                     /locus_tag="VG272_04195"
     CDS             complement(3972..4832)
                     /locus_tag="VG272_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12914.1"
                     /translation="MSISPNVPDPGTHEQNFYEAAPTPRWIMVVFVVAFLLIGYFAYA
                     GNTAKAKLENDLAQANSKEAILSVQIDQTNSRVADLKGQLEVTTQKLGFTQAELAQAR
                     ALAQNIRQSQKESDDKLTTQIGQVKQDTEAKIGQVSTDLSGAKTDISATKQDLEATKE
                     KLTSTVGDLGVQSGLIARNQEELDQLKRSGERNIFELHLAKSKTPTHVGPIQVTLTKT
                     DPKKFKYTLMVVADDKTIEKKDKTADEPVQFYIKGATKPYEIVVFDVTKDKINGYLST
                     PKDVGTARAQ"
     gene            complement(4982..5383)
                     /gene="queF"
                     /locus_tag="VG272_04200"
     CDS             complement(4982..5383)
                     /gene="queF"
                     /locus_tag="VG272_04200"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HWF12915.1"
                     /translation="MADYTDEHACAGVDAKLPSLDTWPNQFPGYKIVTRYPEYTSVCP
                     KTGLPDYGTITIEYEPKKACLELKALKMYLLAYRSLGIFYENAVNRMLRDIVDAIQPV
                     WCEVRGEFTPRGGLTTTVEARWPRNTKSRKA"
     regulatory      5934..6005
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            6229..6606
                     /gene="crcB"
                     /locus_tag="VG272_04205"
     CDS             6229..6606
                     /gene="crcB"
                     /locus_tag="VG272_04205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HWF12916.1"
                     /translation="MTKYWMVGLGGFLGSIARFWLGSYITYRMGARFPFGTFVINISG
                     SFLIGFIVTLLGERAHWSPNLLYLIPIGFIGAYTTFSTFELEAFRNVRTGEVLMALLY
                     VVLSVTVGFIAVWLGVLTGKTLT"
     gene            6961..7674
                     /locus_tag="VG272_04210"
     CDS             6961..7674
                     /locus_tag="VG272_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12917.1"
                     /translation="MFRHDDRFSRFLKAATSITFLAAWGILGVPVLRSAAKQDAGQAT
                     PPPAAPAAPKPVELPDGEGKPIATEYCQDCHRLTNLTKAHKSLDDWKDTIHTMIDRGA
                     RLPDDKIDTLAKYLAANFAPKTDAAPAGGSVPAAAGPAGSPDAGAPATPAPGKVVVEL
                     PEGEGKAIATENCQACHKLTNLTKAHKSLDDWKDTVQLMMDRGANVPPEKVDTLVQYL
                     AKNFGPKDPAAPAAPAPSN"
BASE COUNT         1761 a         2090 c         2411 g         2025 t
ORIGIN      
        1 agaccgagca agctgaaacc cgcgccacca aagccacttc aagcatagca tgcatgaagt
       61 tcaccaatat ttgccgcttg gttataagat gaatgcccgg gaatttcctg cgacgggatg
      121 gaggaaaact agaaacgctg tgactcagaa ttccgactat cgctttaaca attttttatt
      181 ctacggcgtc attttgtgtc ttgcctactt ggtgttccgg gtttttgagc cgtttctcgc
      241 gccgcttggc tgggcggctg tatttggcgt tctgtttcac tctttgaata aacgctttga
      301 aagaaaatgg ggcagcacgc gatcggccgc gctgatcacc ctgggcgtaa cgctgattct
      361 gattattccg gttctgtttc tggcggtaat gtttgtgcgc gaggggattg tggcggccca
      421 agatattcaa agggccgtag gaaacggcca gtacggatgg ttcggccgag cgtgggcgtg
      481 gattgccgct aaaattgcgg cacagggaat cgacctggat cttcccggac ttgtgcggca
      541 aggagccagt agggccgggg agtacatggc cgcggagctt ggacagatca tacgtaatat
      601 tgtgattttt atttttgaat tattcgtcac gctgtttgcg ttgttttatt ttgtaaaaga
      661 tggcgatacg attctcgcac ggttccgcaa ttttttaccg ttcgaggaaa ccacaaagga
      721 gcgcatgctg gcggaggcgc gcgaactggt gtttgccagc gttaccacca gcgtggtgat
      781 cgcgctcgtg caaggattga tttgcggcag cgcgtttgcc ctcgtggggc tcggctctcc
      841 gattttctgg ggcgtggtga tggcattttt atcgctgctg cccgtggtcg gtgcgtggcc
      901 agtgtggatt cccgcagcga tctggctgtt ttccagtggg cattggggcc gggccgtgat
      961 tctgataacg atctgcggcg cggtaggagg aacggtggac aacgttctgc gcccggtgct
     1021 gctgggaggc cgagcgtcac taaatgggtt gctggtattc atcagcgtgc tgggcggaat
     1081 tgaagtgttc ggaatcctcg gagtgattct cggtcctatc gtcgtcgcga ctgccgtcgg
     1141 gattctggat gtgtattccg gaatggatga agccgagcgc acgccgaagg tgctggggtt
     1201 ctgagtggca taggtttcag ccttcgcggg gatagtcctt gcaaggccaa aacccgcgca
     1261 ggctgaaacc tatgccactt aaatccgttt gcagggctgt ggaaattatg aaatgggtga
     1321 ctgtgcgccc gccgtctggc ctgtgctaga gtaacccgct tgagaatgag aagaatttaa
     1381 aaatggccac tgaacctatg atcttgcccg ttggattgcc tgccgaaaaa cttcggtgga
     1441 gatgcgatcc cgcgacgatt ccatttgcga ctacggcgga agccgagccg gttaaggaat
     1501 tgctcggcca agagcgcgcc ttccgcgctc tgaaaatggg cgtcgaagta accgcgcccg
     1561 gcttcaatat ttttgtgtgc ggccttgcag gcaccagtcg cggcggcacg attacgcacc
     1621 tgatcgacca aatccaccct ccgtcgaagg cttcgctcga tcgctgctac gtcaataatt
     1681 ttaaaagtcc ggatcgcccg aggctgattg cactaccgcg agggcaggcc aacaatttca
     1741 agaaagacgt acagagcgga attgaatttt tacgccggcg aattccgcag gtattcgagg
     1801 gcgagccgtt tcagcggcaa aaagggcgga tcgtggaccg cttcacggta cgcgaaaaag
     1861 aattgatgga tgatttcacg catcgcatcg cgcgcgagca atttgccctc ggacacatgc
     1921 aggtgggcgc cgtcgcgctt ccggaaattt ttcctgttct cgaagggcag atggtgccga
     1981 tcgaagatat tccaaagatg gtgcacgaag gcaaattgga aacgatcgtg gccgaagatc
     2041 tggaacgcaa atatgaacag ttccgccagg agttcacagt tgtttatata aaaacgctga
     2101 cactttcgcg ggagcttgcc agcgaattga gttacctcga acaggaagcg gcctccgtgc
     2161 tggtggacgg cttcatcgag gaattgaaag aaaaatatct gggcacgacg gtgtcggagt
     2221 atctggaaga agtgcgccat tcgattctgg ataatctcga tccgttcaag gaacgcgaag
     2281 gcgaggaaga ccctggcggg ggcccgccgc ccgagagcgg ccagaagcct ccgaccaacg
     2341 agcgcgaccc gtttcgagtg tatggcgtga acgtggtgct cgcgcacacc gatgaagaca
     2401 agtcgtccgt tattttcgag acgacgccga cctacacgaa tttattcggc acgattcagc
     2461 gcagttacga ttcgcgcggc ggctggaatt cggatttcat ggacctgcgt cccggatcga
     2521 ttcttcgagc cgacggcggc tttctggtga tgtatgcgct ggacgcgctc accgaagcgg
     2581 gagtctggcg aacgctaaag cgcatgctca accatgcgaa gcttgaaatt caaccgctgg
     2641 aaatgttttt tccgttcgga tcttccgcgc tcaagccgga accgattgac attgatgtga
     2701 agctgatttt aatcggcgac cggcacatgt atgaattgct ttacaatttt gaggaagatt
     2761 tccgcaaaat ttttaaagtc cgcgtggagt tcgatgaaga gatggtgatg agcgacgccg
     2821 tgattcgcca ttatggcgac cgcttacgtc agctttccga gcaggaaaaa atcgggccct
     2881 tcgaccgcac ggctgtggcc agcatgctgg agtatggcgt gcgtctggcc ggacggcgca
     2941 acaaaattac ggcgcgattt cctgagcttg cggatctggc gcgcgaggcg gcgtacatcg
     3001 cgcggcaaga cagttcagat gtggtgaatg gcgcacatgt gcggcgcgcg cgcgaagccc
     3061 gcatcgagcg gcacaacctc acggaaacca aaatccgcga aatgatcgaa gagggaacgc
     3121 tgatgattga aacggatagc gcgcgcgtcg gccagatcaa cggtctgagc gttctggaaa
     3181 ttggcggcta tgcgtttggc aagccggtgc ggatcacagc ttccgcgtcg cttgggaaaa
     3241 cagggctcat caacgtggag cgcgaggcga atctcagcgg cagtttccat gacaagggaa
     3301 tgcacatcat cgccggatat ctgcgaatga agttcgcgca ggataagccg ctttcactcg
     3361 cggccagtat ctgctttgag caatcgtaca gcggcgtcga tggagacagc gcaagttcca
     3421 ccgagattta cgcactgctt tccgcgcttt cgggattgcc gattcgccag gacattgctg
     3481 tgacgggctc cgtcagccag caaggcgaca ttcagcccat cggcggagtg aaccagaaga
     3541 tcgaaggctt cttcgatgtg tgccgcgtga agaccgggct aaccggtcgc cagggcgtga
     3601 tgattccgta cgagaatatc gaggatttaa tgctacgcga cgatgtgatc gaggctgtcg
     3661 ccgcaggaaa atttcacgtt ctaccggtaa aaacactgga agaggggatc gaaattttaa
     3721 cgggtgtgaa ggccggacgc cgcgatgctg gtggagtttt tgaagccggt acggtgtatg
     3781 cgcaagtgga tcggcggctc cacgagatgg ctacgacgat gaaggacttc cagtcgtagg
     3841 ttgcagctta ttcaaaacca atcaaactaa aaagtcatgc ccgcttgaga agtagcacag
     3901 gctttagcct gtggggttta gtccttgcaa ggcctaaatt ccacaggttg aagcccatgc
     3961 cacaaagacg cctattgcgc gcgggccgtg cccacgtctt tcggcgttga gaggtagccg
     4021 ttaattttgt cttttgttac gtcgaagact acgatttcgt agggcttggt tgcgcccttg
     4081 atataaaact gcacgggttc atcggcggtc ttgtctttct tctcgatggt tttatcgtcg
     4141 gccactacca tcagggtgta tttgaatttt ttgggatcgg tcttggtcag cgtcacctga
     4201 atcgggccaa cgtgtgtcgg tgttttggac ttggcgagat gcagctcgaa aatattccgt
     4261 tcgccggagc gcttcagctg atcgagctct tcctggttgc gcgcaatcaa gccgctttgc
     4321 acccccaggt cgcccacggt acttgtgagt ttctctttgg tggcttccag gtcctgtttc
     4381 gtcgcgctga tgtctgtctt cgcgccggac aaatccgtgc tcacctgccc gatcttggcc
     4441 tcggtatctt gcttcacctg tccgatttgc gtggtgagtt tgtcgtctga ttctttctgg
     4501 ctctggcgga tgttctgggc gagagcgcgc gcctgcgcca gttccgcttg ggtaaatcca
     4561 agcttttgcg tggtcacttc cagttggccc ttcagatccg caacgcggga attggtctga
     4621 tcgatctgca cggaaagaat cgcttccttg gaattggcct gcgcgaggtc attctccagt
     4681 tttgcctttg cggtgttacc ggcatacgcg aagtacccga tcagcaaaaa tgccaccacg
     4741 aataccacca tgatccagcg gggcgtgggg gccgcttcat agaaattttg ttcgtgggtt
     4801 cccgggtcgg gaacgttcgg ggaaatgctc atcgtctctc cttagcggcg ggtgttacgg
     4861 cgcgcgtgcc gtgaattctc gcgagctgta agaccgccgc atcctaaaac tgtaaaagtg
     4921 aatgtgagtg cgcaaccgcc actcacgtag ttcagattat tactgagtgg ctataggttg
     4981 tctacgcttt gcgggacttc gtgtttcttg gccaccgcgc ttcaaccgtg gtggtcaatc
     5041 cgccgcgagg cgtgaactca ccgcgaactt cgcaccacac cggctggatg gcgtccacaa
     5101 tatcacgcaa catgcggttc accgcatttt cgtagaaaat gcccaggctg cgatacgcca
     5161 gaagatacat ttttagcgcc ttcaattcca gacaagcttt ttttggttcg tactcaatgg
     5221 taattgttcc gtaatccggc aatccggttt ttgggcacac cgaggtgtac tcgggataac
     5281 gggtaacgat tttgtatcct ggaaattgat tcggccaggt gtccaggctc ggaagttttg
     5341 cgtcgactcc agcgcacgcg tgctcatccg tgtaatcggc cattttgctc cctctggagc
     5401 tattgtacat gcggcagaac acctcgcgta gttggaactt gccaattcaa tccacgttct
     5461 attattcttt cggcagaatt ccaggggaac cagaacttgt tatgtcccgt ttgcctgagc
     5521 gatagtcctc ggcggtctcg caggcgcagc gcgaaggatt attgcgttgg tgttgcacgg
     5581 ctgcgaccgt ggagatgtaa aatttgcgac tcgcggttct acgcgtgggc cacttcgccc
     5641 ggctatgtct ggtatgttca ttgcggcatg tgcggcaatc tggatgtgca gcgtatttcg
     5701 agcgagcacg gaatgggcat gctttcgtgg atctatcgac tgttccgcgc gcccgcttac
     5761 cgctgcgcgc cctgccgcaa tcgctttttt tcattccgga tttacaggcg tattatgcct
     5821 tccaaacaac ccgtggaacc acggcccgat tcgatccctg ttcatcactg aagcgtgatt
     5881 gaaccggaag tctcgtagat ccggcaatgg acttccattc cgtcaaacgt atgataacca
     5941 gaggcgatgg agttcgcctt ataaccgctg tccttcgccg gacagatgat gactcctgac
     6001 aagttccttg tcgcggagtc atgtcccggc aatgaaccga tttaattcac caagcctttg
     6061 atcgagacgc gctgcataga atttttcgtg ctgccacaca acgaaaaacg gccgttgtgc
     6121 cgacgtaccg ccggtatttt tagacgacac gtgccgacgg acagaaaacc gccggcaaga
     6181 ttcgggcgtc agaacacgga acgaacgaaa gtttggacga ggacgcggat gacaaaatat
     6241 tggatggtcg ggctgggggg attccttgga tcaattgcgc gattctggct gggttcttac
     6301 attacctatc gaatgggcgc gcgttttccg tttggcacgt ttgtaatcaa catcagtggc
     6361 tccttcttga ttgggttcat cgtgaccttg ctcggcgaac gtgcccactg gagtcccaac
     6421 ctgctttatc ttattcccat cggttttatt ggagcgtaca cgacgttctc cacgtttgag
     6481 ttggaagcgt tccgaaatgt tcgtaccgga gaggtgctta tggcgctcct gtatgtcgtg
     6541 ctcagcgtaa cggtgggttt tatcgcggtg tggctcggcg tgctgacggg gaagacgctg
     6601 acttgaacgc tgtgggcgtg gactcaggta tcgtaaatat tacgggggca ggcgcgactg
     6661 gcggtgaaat gcccactcgg tccccaaaaa cgggacagag tggcctctac aagaacatag
     6721 tgcgcgcacg tcttcgtttt tgtagcggcc gaccgatttt tgggcgggcg ctttttcatt
     6781 gctacgaccc aacctatgca ttcacgtcag gatgttgcgc tcgcttgatt cttttcttcg
     6841 ttcatatcaa aatgaaaatg tggagtttat cgtttttgag ctttggatgt gtgctgtaga
     6901 accagcgaaa ttcgtaagcg aacttatagc ccgcgttgaa aatccctgga ggattcacca
     6961 gtgttcagac acgatgaccg tttttcacga tttttgaagg cggcaacgag catcaccttt
     7021 cttgcggcgt ggggaattct tggggttccc gtgctgcgtt ccgcggcgaa gcaggacgcc
     7081 ggacaggcca ctcctccgcc ggcggctccg gctgctccga agcctgtgga attgccggat
     7141 ggcgaaggga aacctatcgc caccgaatat tgccaggact gccatagact tacgaatctg
     7201 acgaaggcgc ataagtcgct ggacgactgg aaagatacga tccacacgat gattgaccgc
     7261 ggcgcgcgcc tgccggacga caagatcgat acgctcgcaa aatacctggc agcgaacttt
     7321 gcgcccaaaa cggatgctgc accggcgggc ggctcagttc ctgccgcagc aggccctgca
     7381 ggctcaccgg acgctggcgc gcctgccaca cccgcgcccg gcaaggtagt tgtggagctg
     7441 cctgagggcg agggaaaagc gattgccacg gaaaattgcc aggcttgcca taagctgacc
     7501 aatttaacga aggcgcacaa gagtctggac gattggaaag ataccgtcca gctgatgatg
     7561 gatcgcggcg cgaacgttcc tccggagaag gtggacacgt tggttcaata tctggcgaag
     7621 aacttcggtc ccaaggatcc cgctgcacct gccgcgcccg caccgtcgaa ttaagcgcgc
     7681 gtttctctgg cgaagagagt cattgccgat tcaggctgcg gctcagcctc ataatttata
     7741 agaaaaatcg cgtggcatgg tcatggtcga tgaacacctt gattactgac cttttgtttt
     7801 tacggattgc ttgcgcctgc ggggtgtccg tgcgaaaatc tcccctcaat cgactgcgct
     7861 aatcgactgg gctaaacata gctatacgta tgtcaggcgg acgcgattgg ctcacgacgg
     7921 cgatatcgtc taagggttag gacgtggccc tctcaaggga attacggtcc tttgatggcc
     7981 ttaggcgacc ttagatcatt caacaagaca ctgaaaataa acgctttata tattctgaat
     8041 cgcggttggc ttaggctggc ttaggtggtt ttgatcgttt tttctaaaat ctgatgcaag
     8101 tctgatgcaa gttgatgcta gtcggagcga atggacggcg aagtagaaaa gccgttactg
     8161 agtgggctgc ttcgggtgta agctgttttt gcgattttgc tgccgacggc aatccgtttc
     8221 ttcaaaattc aagcttgttt gctaagtctt taggtgcggt agtgcttcgt tcaacgccgc
     8281 aatgaat
//
LOCUS       DASZMB010000091         4963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3648668, whole genome shotgun sequence.
ACCESSION   DASZMB010000091 DASZMB010000000
VERSION     DASZMB010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3648668"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VG272_04215"
     CDS             complement(<1..508)
                     /locus_tag="VG272_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HWF12918.1"
                     /translation="MTQRELTTQDYVAMLRRRWVLIVVLTVLGPPFTYGVSKLLPNRY
                     KSQTLVLVSQPTVASDLVKPVDTSDMSQRLSSMQQQILSRSRLEPIIRQYGLFPNDAN
                     RKPMEYLVAQLQKAIEVTPILPMAETRANNLPGFYVNVTLSNPKTAQAVCNTITSMFI
                     DENVHLRQQ"
     gene            1197..1973
                     /locus_tag="VG272_04220"
     CDS             1197..1973
                     /locus_tag="VG272_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWF12919.1"
                     /translation="MKGVVLAGGTGSRLNPLTRVTNKHLLPIYDKPMVYYPIQTLVNA
                     GIEEILLVTGGKNAGDFLRLLGNGRDFGLKHINYTYQEGEGGIAQALGLAEFFAGDDP
                     ICVILGDNIIEGNICQAVENFKKQDGGAKILLKEVTDAQRFGVAELRGNHVVGIEEKP
                     KKPKSNYAVIGIYLYDSTVFQKIRRLKPSGRGELEITDVNNFYGAEDKLTFEVLDGWW
                     TDAGTFESLLRANQLVAETGANKTNQTMVGEPVGSREVKR"
     gene            1970..3022
                     /gene="rfbB"
                     /locus_tag="VG272_04225"
     CDS             1970..3022
                     /gene="rfbB"
                     /locus_tag="VG272_04225"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR01181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HWF12920.1"
                     /translation="MKIFVTGGAGFIGSNFIRLVLSQRKDYDIVNYDKLTYAGNLANL
                     DTVADNPKYSFVRGDICDAAALEEAMRGCTAVVHFAAESHVDRSIYEPAPVIATNVTG
                     TFFMLQVARKLNIERFVHISTDEVYGDMLPGAFATEESPLQPSSPYSASKAASDLLVR
                     SYVRTYGFPGIITRSSNNYGPYQFPEKFLPLMITNAFDDKPLPIYGDGKQQRDWLHVE
                     DNCRGVLAVLERGRIGEVYNIGGTDVEENLTIVRKVLKSVGKPESLLTPVKDRPGHDR
                     RYALTTEKMEKELGWKPEIPLDEGLRRTIDWYRTNAKWMAGVRGGEYLSYYEKYYENR
                     DSSLHALTSSGTKHPK"
     gene            3128..3679
                     /gene="rfbC"
                     /locus_tag="VG272_04230"
     CDS             3128..3679
                     /gene="rfbC"
                     /locus_tag="VG272_04230"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HWF12921.1"
                     /translation="MPTSLRDVWELQPKVFRDPRGFFLETYNKQRFADLGIHENFVQD
                     NHSRSSQGTLRGLHYQLHHAQAKLCRVIEGEAFDVAVDIRVGSPTFGKWASVLLSAEK
                     QNQIFIPAGFAHGFLARTQTVQFLYKCSEFYNPSDEHGISWNDPGLAITWGISNPLIS
                     EKDTKYLTLAEASPENLPRYSKP"
     gene            3676..4605
                     /gene="rfbD"
                     /locus_tag="VG272_04235"
     CDS             3676..4605
                     /gene="rfbD"
                     /locus_tag="VG272_04235"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HWF12922.1"
                     /translation="MKPAILLIGNNGQVGRELSRMLPQLGEVKSLGREQLDLSNTEEI
                     RSALRAIRPQLIVNAAAYTAVDKAESEQDLARAVNAVAPAVMAEEAKTLGALLVHYST
                     DYVFDGTKTSPYAEDDPTNPKNIYGKTKLEGELAIQASGVSHLIFRTAWVYGTEGKNF
                     LLTILRLATQREELKIVCDQTGAPTTSAEIAIGTTKILSQMFAQADRPSRALDGGVFH
                     MTAGGVTTWFGFTQAILEHARKMDSREAWFAAATAERPLMVRNVVPIPAVQYPTPASR
                     PCYSVLSNAKMDRVFSVRLPDWQTQLASIFAKS"
     gene            4768..>4963
                     /locus_tag="VG272_04240"
     CDS             4768..>4963
                     /locus_tag="VG272_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HWF12923.1"
                     /translation="MAETILRSNIEGSIQVHSRLLEVCLPAMTAAANALVAAYRTGHK
                     ALFFGNGGSSSDAQHLAAEFV"
BASE COUNT         1212 a         1273 c         1379 g         1099 t
ORIGIN      
        1 gctgctggcg cagatgtacg ttttcgtcaa taaacattga cgtaatcgtg ttgcaaacgg
       61 cctgggcagt tttaggattg ctcaaggtca cattgacgta gaaacctggc aagttattcg
      121 cgcgagtttc cgccatgggc aagatcgggg ttacttcgat cgccttttgc agttgtgcga
      181 ccagatattc catcggtttg cgattggcgt cgttcgggaa caagccgtac tgacgaatga
      241 tcggttccag gcggctgcga ctcaggattt gctgctgcat gctggagaga cgctggctca
      301 tgtccgatgt gtctacgggt tttaccaagt cagacgcgac ggttggctgc gaaaccagca
      361 ccagagtctg cgacttgtat cgattaggga gaagcttcga aacgccatag gtaaacggcg
      421 gccccagtac cgtcaaaaca acaataagaa cccatcgccg ccgaagcatc gcgacgtaat
      481 cttgggttgt caattcgcgc tgcgtcattt cgacctcatt cccaggctct gaatatcttg
      541 tatcacttgg cacctttact aaaccgcgtt ctgcgccctg tcaagtcgac gaaagtactg
      601 cacctcggcc gggcgctcag acagacatct gtcgccgtca ggaccgactc ggaagacaaa
      661 tccttcgatt tccattgaat ccagagagct taaccttgcg attccgagaa aataacgaca
      721 cgcacggata agctttctcg tggacatgac ttgcaaggcg gatgccaccg ttacgggaag
      781 cgtggtgccg tggcattggg tcgtaatgat tttgtttaca aagagttacg tgcaatggcc
      841 aggcgctgat gggatatatc ggaagcaagg gagtccgcta ctagttccat gaataggcaa
      901 atgttttcca ttgtttcttg ccaatccacg ccaacagagg caatacgagc catccctatg
      961 ctaaccttgc acttccaagc ccgtgttcga cttcattacc gtcctcggtt acctagttta
     1021 aaagaggtta ctaatggtct ccacaacctg aaaaaggtat ttgtccgcat tttgaaaggt
     1081 gctatcacat attgggacgt caacacggtt ccttccggct tttcatcaga caggatcctg
     1141 attcccacga aatcgcgtag attaaaggcg ctataccaag aggagcacag acaattatga
     1201 aaggtgtcgt cctggcaggc ggaaccggga gccgcttgaa tccccttacc cgagttacga
     1261 acaagcattt gctgccgatt tatgacaagc cgatggtgta ttacccaatc caaactctgg
     1321 tgaacgcggg tatcgaagaa atacttttgg tcacaggcgg caagaatgcc ggagacttcc
     1381 tcagactgct cggaaacggc cgcgacttcg gattaaagca cattaattat acctatcagg
     1441 aaggcgaagg cggcatcgcg caggcgctgg gactagcgga atttttcgca ggcgacgatc
     1501 ccatttgcgt gattctggga gacaacatca tcgagggcaa catctgccag gccgtggaaa
     1561 atttcaaaaa gcaggatggt ggcgcgaaga ttctgctgaa agaagttacg gacgcacagc
     1621 ggtttggcgt cgcggagctt cgtggaaacc acgtggtagg cattgaagaa aagcctaaaa
     1681 agccaaagtc gaactacgcc gtgattggga tttatttata tgacagcacc gtgtttcaga
     1741 aaattcggcg cctgaagcca tcgggccgcg gcgaactgga aattacggac gtgaataatt
     1801 tctacggcgc ggaagacaag ctgacctttg aagtactgga cggctggtgg acggatgccg
     1861 gaacgtttga atcgctactg cgcgccaatc agctggtggc ggaaacaggc gcgaataaaa
     1921 cgaaccaaac gatggtcggg gagcccgttg ggtcccggga agtgaaacga tgaaaatatt
     1981 tgttacgggc ggggcgggct tcatcggctc aaattttatc cggctggttc tttcacagcg
     2041 taaagattat gatatagtta attacgataa gctaacctac gccggaaatc tggcaaatct
     2101 cgatacggtt gccgacaatc cgaaatattc gtttgtacgc ggagatattt gcgacgccgc
     2161 ggcactggaa gaagccatgc ggggctgcac ggctgtcgtc cactttgcgg cagagtcgca
     2221 tgtggaccgc agcatttacg agcccgcgcc ggtgatcgcg acgaacgtga ccggcacgtt
     2281 ttttatgctg caggtcgcgc gaaagctgaa catcgaaaga ttcgtgcaca tctccacgga
     2341 cgaagtctac ggcgatatgt tgccaggcgc ttttgccact gaggaatcgc cgctgcagcc
     2401 gagcagtccc tattccgcgt ctaaagcggc atcggattta ctggtgcgct cctacgtgcg
     2461 cacatacgga ttccccggaa taatcacgcg ctcctccaac aattacgggc cgtaccagtt
     2521 cccagaaaaa tttctgccgt tgatgatcac caacgcgttt gacgacaagc cgctgccaat
     2581 ttacggcgac ggaaagcagc agcgcgattg gctgcacgtg gaagataatt gccgcggcgt
     2641 tctggcggtt ttggaacgcg gcagaatcgg cgaagtttat aatatcggcg gaacggacgt
     2701 ggaagaaaac ctgaccatag tccgcaaagt tctgaagtcc gttggcaaac cggaatctct
     2761 gcttacgccc gtgaaagatc gcccgggaca cgaccgccgg tatgccctga ccaccgaaaa
     2821 aatggaaaaa gaactgggct ggaagccgga aatcccgctg gacgaaggct tgcggcggac
     2881 catcgactgg tatcgcacga acgcaaaatg gatggccggc gtacgaggcg gcgaatacct
     2941 ttcttattac gaaaagtatt atgaaaaccg cgattcctcg ctgcacgcgc tcacctcaag
     3001 cgggacgaaa catccaaagt aaacgggact gacgctgact gtattcagga ttgagatttt
     3061 ttaattttaa ctgtgatcgt cctgagaaaa actccgattt tgtaaggaat ggttccgatt
     3121 gcaacgagtg cccacatcgc tccgggacgt ctgggaactt cagcccaaag tgtttcgtga
     3181 tccccgcgga ttttttctgg aaacatacaa caagcagcgc ttcgcagact tgggaatcca
     3241 cgaaaatttt gtacaggata accactcgcg ctcctcgcaa ggaactctcc gcgggctgca
     3301 ctatcaattg caccacgcgc aagccaagct gtgccgtgtg atcgagggag aagccttcga
     3361 cgtggccgtg gatattcgcg tgggctcgcc gacatttgga aaatgggcga gcgtactgct
     3421 ctccgcggaa aagcaaaacc aaattttcat tccggctgga ttcgcgcacg ggtttttggc
     3481 ccggacgcaa acggttcagt ttctctacaa atgcagcgaa ttttataacc cgagcgatga
     3541 gcatggaatc agctggaacg atcccgggct ggcgatcacg tggggcattt ccaatccgct
     3601 gatctcagaa aaagatacaa agtatttgac gcttgccgaa gcctcgccag aaaacttgcc
     3661 gcgatattcc aagccatgaa acccgccatt ctccttatcg gaaacaacgg tcaagttggg
     3721 cgcgaattga gccggatgct gccgcagctc ggcgaagtaa aatctctagg ccgggaacaa
     3781 ctggatcttt ccaacactga agaaattcga agcgcgcttc gggcgattcg ccctcagctg
     3841 atcgtgaatg cggcggcgta tacggccgtg gacaaggccg aatcggaaca ggacttggcg
     3901 cgcgctgtga atgcggttgc tccggcagtg atggccgagg aagcgaaaac gctcggcgca
     3961 ctgctggttc actactcaac cgattatgtt ttcgatggca caaagacgtc cccttacgcc
     4021 gaggacgacc cgaccaatcc aaaaaatatt tatgggaaga cgaagctcga aggagagctg
     4081 gcgattcaag cttccggcgt ctcccacctg atttttcgca cagcatgggt gtatggaacc
     4141 gagggcaaga atttcttgct gaccattctt cggctggcga cgcagcgcga agagctgaaa
     4201 attgtttgcg accagaccgg cgcgcccacg acgagcgcgg agatcgcgat aggcacgaca
     4261 aaaattctgt cccaaatgtt tgcgcaagcg gatcgaccgt ctcgcgcgtt ggatggcggc
     4321 gtatttcaca tgaccgcggg cggcgtaacg acttggtttg gttttacgca ggcgattctg
     4381 gagcatgcgc gcaagatgga ttcgcgcgag gcgtggttcg cagcggctac agcggaacgt
     4441 ccattgatgg ttcgcaacgt tgtgcccata cccgctgtgc aatatcccac tcccgcgagc
     4501 cggccttgct actctgtttt atcgaacgca aagatggacc gcgtattttc tgtgcggctg
     4561 ccggattggc agacgcagct ggcttcgatt tttgcgaaat cgtagcggtg ctctgtcggc
     4621 aggcgatttc tacgtcgaat cagaattctg acccactcaa ttcagctgaa ttgtttgctg
     4681 cgcgcttcgg aaaaccgcca gcgggacgcc ggcgctacat ttggcccggg aaatgttata
     4741 aatttggtgc cgggcacgga ggcgcacatg gctgaaacca ttcttcgctc caacattgaa
     4801 ggcagcatcc aggtgcacag ccggctgctg gaggtgtgtt tgccggccat gacggcggcg
     4861 gcgaatgcgc ttgtcgctgc atatcggacg gggcacaagg ctttattttt tggcaacggt
     4921 ggaagctcgt ccgacgcaca acatctggcg gcggaatttg tcg
//
LOCUS       DASZMB010000092         6325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_908292, whole genome shotgun sequence.
ACCESSION   DASZMB010000092 DASZMB010000000
VERSION     DASZMB010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6325
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908292"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VG272_04245"
     CDS             <1..890
                     /locus_tag="VG272_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12924.1"
                     /translation="SKRLSHGVSFVAGYTYGHGLDNGSLNRFGLLPQNPNNPGGEYGN
                     SDFDVRHRLTLTGTYNIPGIKGFAQVLEGWQLNGILTLQSSQPWVVNDYGNDFSGVGD
                     SSDRWDFFGNPGDFKGTQNSIPYCTSGGLGGCTTTDGVYGVVTKMSASQSTSMWNQCT
                     TINAAHPTTTQLVGVGCYASGSSVMLAPANGTFGTMGRNIFRDSGFKNVDFSVFKNFK
                     YKERYSAQFRLEVFNVLNKPVIANPYGASNGSSGGNNDPSASSFFGGAIGTPDFIAGN
                     PVVGSGSNRDVQVGLKLQF"
     gene            1051..1124
                     /locus_tag="VG272_04250"
     tRNA            1051..1124
                     /locus_tag="VG272_04250"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1083..1085,aa:Cys,seq:gca)
     gene            1682..3400
                     /locus_tag="VG272_04255"
     CDS             1682..3400
                     /locus_tag="VG272_04255"
                     /inference="COORDINATES: protein motif:HMM:NF028240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HWF12925.1"
                     /translation="MRNYKLSIVRAPAVSILAVLITALTGTLPTFAANEDSPGSTAMP
                     FAASKEKVGANGANGATPAATPIAALVEGRVPTPSVEQVMDLVQEQGQEIAALRAALR
                     EQQELSARLEARLDAGPPAPVVAAAVALTPAISAASLAVGSTQTELEQKIAQIETNIG
                     KSQKNLEERIKKIGPFTFSGDFRLRAEPTFGGPLDQSQQRFRQRVRLRFNVETKLNDQ
                     IRGGFSVASGDLNSPISTSQTLNQFESRKPLAIDRMYATYTPSWFKPLTLTGGKFMYT
                     WFNTEMVWDKDLNPEGASETLSFNVKTPALKNISFVGFQSPVAKVQRTPANDKSYYNT
                     VVYGEQVQTYWQLGERVRLSAYESFYDWKFADSVAFSLLEANSASPEDGLLPLANLGE
                     VNSIATLTSTNSITGAKTLASAQFASKFAILDSLAEIDVATRSERWPITFIGDFAENT
                     RACENVKNISLFAVYSAPCDPHARHGYWLETSVGRARKRGDWQFSYTRMMIQREAVLG
                     AFNYSEIFPTSNVEMHRPEISYQLFDNVTLQLNGLFGRPLVNAGSPAPVQPLWERLQF
                     DMTYKF"
     gene            3440..4471
                     /locus_tag="VG272_04260"
     CDS             3440..4471
                     /locus_tag="VG272_04260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042918 - alkanesulfonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HWF12926.1"
                     /translation="MKTTEAHPALLLAAIMLLAGSGVRAQQKPLVIRVGYFPNITHAQ
                     ALVGRAGGQFDKALGTGVQMEWKPFNAGPAAVEALFAGAIDITYIGPNPAVMGYVRSE
                     GEAVRLIAGAASGGAALVVREGAAIQNISDFRGKKIATPQQGNSQDVALRAWLHASGL
                     KTMDKGGDVEVLPINNAYQYTLFLKGELDAAWSPEPWASRLIHEAGGRMFLDERDKWP
                     NHEFASTVLIVRPKFLKEHPDLVKSFLRAHLELTDWINSNPDQAKLIINQQLKKETGK
                     PLKQAVLDDAFSRIKVTYDPIRSSLVTSTQEAFEAGFLGHKQPDISGLYDLTLLNEVL
                     REKKAQPLQ"
     gene            4468..5409
                     /locus_tag="VG272_04265"
     CDS             4468..5409
                     /locus_tag="VG272_04265"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF12927.1"
                     /translation="MTRYVIDLFNRSIGGGLVRAENDELPEPLKVEKKSSSESRPKSL
                     PEHSAKESIELSGVFRSYKNSRGSFTPALQNIDLEIEQGEFVCIVGPSGCGKSTLLHL
                     IAGLDKPTTGQITVDGGPVTGPGTDRILLFQELGLFPWLNVRQNVEFGLKMAGVSNEE
                     RKERARIFLRMVHLSHFEDHYIHQLSGGMRQRVALARSLALRPKILLMDEPFAALDAQ
                     TRDMLHDELERIWKETAPTIVFVTHNVREAVRLGDRVLLMSFRPGRIKAQFEVSLDRP
                     RHVEDSDVAQLSKEILGQLREEIDRSFNAEYSREEKR"
     gene            complement(5484..>6325)
                     /locus_tag="VG272_04270"
     CDS             complement(5484..>6325)
                     /locus_tag="VG272_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWF12928.1"
                     /translation="GNEEQKKKWLPAMAQGELIGAIAMTEPSAGSDLQAIKTTARRDG
                     NEYVISGSKTFITNGGVANLVCVAAKTNPKVAGFRGISMIMAETKNLPGYRVGKSLEK
                     VGMHEIDTCELFFDDVRVPAANLLGKSEGAGFAQMMDQLRYERLMISVGAIAAIERAL
                     ELTIHYAKERKAFGQTLMEFQNARFKLAHAKTEAQIGRVYVDNCVMQFLAGKMDPVTA
                     AMVKVWTTECQSRILDECVQMFGGYGYMSEYPIARMWMDGRVQRIYAGSNEILVELIG
                     SSL"
BASE COUNT         1454 a         1739 c         1715 g         1417 t
ORIGIN      
        1 tgtccaagcg tttatcccac ggcgtttcat tcgttgccgg gtacacgtac ggtcatggtc
       61 ttgacaacgg ctccttgaat cgcttcggtc tgcttccgca aaacccgaac aatcctggcg
      121 gagaatacgg caacagcgac tttgacgttc gccaccgctt gacgctgacc ggaacctaca
      181 acattccagg gatcaagggc tttgcacaag tgctcgaggg atggcaactt aacggcatcc
      241 tcaccttgca gagttcccag ccttgggtgg tcaacgacta cggcaatgac ttcagtggtg
      301 ttggagactc ctcagatcgt tgggatttct tcggcaaccc cggcgacttc aagggaactc
      361 agaactctat tccctattgc acgagtggtg ggttaggcgg ctgcaccacg acggacggtg
      421 tttacggtgt tgtgacgaag atgtccgcgt cccagtccac ttccatgtgg aaccagtgca
      481 ctacgattaa tgctgcacat ccaactacca ctcaactggt cggtgttggt tgctacgcaa
      541 gcggaagttc cgtgatgctc gcccccgcaa acggaacatt cggcacaatg ggccggaata
      601 tcttccgcga ttccggtttc aaaaacgtcg acttctccgt gttcaagaac ttcaagtaca
      661 aagagcgcta ttccgcgcaa ttccggcttg aagtctttaa cgtgctcaac aagcccgtta
      721 ttgccaatcc ctatggagct tctaacggct ccagcggcgg aaacaatgat ccttcggcgt
      781 cctcgttctt cggcggcgca atcggcactc ctgactttat cgcgggtaac ccggtcgtag
      841 gctctgggtc gaaccgcgac gttcaggttg gcctgaaact ccagttctaa actcacaaat
      901 gcatccagcc gggaaattcg gtccggcaaa aacgagggag gcaaaaatcg cctccctctt
      961 ttttttgcta cacgtgcaac aaaaccgttc gtagctaaag acgatgcgtg gaatcttctg
     1021 gtagaatcat tgcgcatcgg attgcgatat ggcgcggtcg ccaagtggta aggcaaaggt
     1081 ctgcaaaacc tttatcggcg gttcgatccc gccccgcgcc tccaacctcc cagtaggaca
     1141 ggcttcagcc tgtcttcttt tgaggtctta ttctattttg aagatgcgcg acaaaattca
     1201 aaaccagaca agctgaacag gctgccgaaa aagtcgatgc ggccgagaat ttagtagcac
     1261 aggcactcct gcctgtgcgg tttttggatt tgcgtcgatt cggcggcccg aagttttgag
     1321 aaaagccaca caggcagcag cgcctgtgct gctttttccg caggctgcga agcctgccct
     1381 accggaagcc aggattgact tcccaaccac gtttcagtac ccttgacccg tgaacttcaa
     1441 tgtcctgaag acgaagcagg cctgtctctg ttgtacctgc tgtatctgtg tgtcttctgg
     1501 cgcggggacc taatactcta gaattatatt gagcacttct agaatcccac cgccgaaata
     1561 atctggcggt gggattttta tttttagtgg catcgatttg ggtcgatcct gtcccgtaac
     1621 tgggcagaaa gagaaaggcc tggcaatcgc tccaagttat ggcctgagcc aaaaagtgaa
     1681 catgcgaaat tacaaattgt cgatcgttcg cgcgccggca gtttcgatct tggccgtgct
     1741 gataacggcg ctcaccggca ctctgcccac ctttgcagca aatgaagatt cgcctggctc
     1801 aaccgctatg ccgttcgcag ccagcaagga aaaggtagga gcaaatggcg cgaatggcgc
     1861 aacgccggcg gcgactccca tcgccgcctt ggtggaaggt cgcgtgccaa cgccttcggt
     1921 cgagcaagtt atggatctgg tgcaggagca aggccaagaa atcgcagcgt tgcgcgcagc
     1981 gcttcgtgag cagcaagaac tgagcgcgcg cctcgaagcg aggctggatg ccggtcctcc
     2041 cgcaccggtc gttgccgccg ccgtggcgct aacgccagcc atttccgctg cgtcactcgc
     2101 ggttggatcc acgcaaaccg agctggagca aaagatcgcg caaattgaaa ctaatatcgg
     2161 caaatcgcaa aagaatctgg aagagcgcat aaaaaaaatc ggcccgttta cgttcagcgg
     2221 agatttccgg ctgcgtgcgg agccgacttt tggcggtcca ctcgaccaat ctcagcagcg
     2281 cttccgccaa cgtgttcgtt tgcgcttcaa tgtggaaacc aaattgaacg atcagattcg
     2341 aggcggtttt tcggttgcct ccggcgacct gaatagcccc atttccacaa gccagactct
     2401 caatcaattt gaatcgcgca agccgctcgc gattgatcga atgtacgcaa cgtacactcc
     2461 gagctggttc aaaccgctta cgctgactgg cgggaaattt atgtatacat ggttcaacac
     2521 ggagatggtt tgggacaagg atttgaatcc tgagggcgcg agcgaaaccc tttctttcaa
     2581 cgtcaagacg cctgcgttaa aaaatatcag cttcgtagga ttccaatcgc cggttgcgaa
     2641 agtccagcgc acgccggcga atgacaagag ctattacaac acggtcgtgt atggcgagca
     2701 ggtgcagacg tactggcaat tgggagagcg cgttcgcttg agcgcatacg aaagttttta
     2761 cgattggaaa tttgccgatt ccgtggcgtt ctctctgcta gaggcaaata gcgccagtcc
     2821 ggaggacggc ctgcttccgc tcgccaacct cggggaggta aattcgatcg ctacgctcac
     2881 ttcaacaaat tccatcacag gcgcgaaaac actggcgtcc gcgcaatttg cgtcgaagtt
     2941 cgccatcctt gactcgctgg cggaaataga cgtcgcgacg cgatcggaac gttggccgat
     3001 tacattcatc ggcgattttg cggaaaacac gcgcgcctgt gagaacgtaa aaaatatttc
     3061 tctattcgcg gtgtactccg cgccatgcga tccccacgcg cgccacggat attggctgga
     3121 aacgagcgtc gggcgcgctc gcaaaagagg cgactggcag ttcagctaca cgcgcatgat
     3181 gattcaacgc gaagccgttc tcggcgcgtt caattacagc gaaatatttc ctaccagcaa
     3241 tgtcgaaatg caccggccgg aaatttctta ccaactgttc gataacgtga cgctgcaatt
     3301 gaacggatta tttggcaggc cactcgtgaa cgctggaagt ccggcgcccg tgcagcccct
     3361 gtgggagcgt ctgcaatttg acatgacgta caaattttag gtgaccaaag tgaaagcaaa
     3421 tttattgccg cgactcatct tgaaaacgac ggaagcgcat cccgcgctgc tgctggcggc
     3481 gatcatgttg ttggccgggt ccggagtgcg tgcgcagcag aaaccgctcg tgattcgcgt
     3541 aggatatttt ccaaacatca cccacgcgca agctcttgtc ggtcgcgctg gcggccagtt
     3601 tgacaaagct ttgggaacgg gcgtgcagat ggaatggaag ccgttcaatg ccgggcccgc
     3661 ggccgtcgaa gccttgttcg ccggcgcgat tgacattact tatattggtc cgaatcccgc
     3721 ggtcatgggc tacgtgcgct cggagggtga agccgttcgc ctcattgcag gcgctgcgag
     3781 cggaggcgcg gccctcgtgg tccgggaagg cgcggcgatt caaaacatct cagacttccg
     3841 cggaaaaaaa atcgccacgc ctcagcaagg caattcacag gacgtggctc tgcgcgcgtg
     3901 gctgcacgcc agcggactga agacgatgga taagggcggc gacgtggaag tgctgcccat
     3961 caacaacgcc tatcaataca cacttttttt aaagggagag ttggacgcgg cgtggtcccc
     4021 cgagccgtgg gcttcgcgtt taatccatga ggcgggtggc cgcatgtttt tggacgagcg
     4081 cgacaaatgg ccgaatcacg aattcgccag cactgtttta atcgtgcgcc ccaaattcct
     4141 gaaggagcat ccggaccttg taaaaagttt cctccgcgca catttggagc tgacggattg
     4201 gatcaacagt aatcctgatc aggcgaagct aatcattaac cagcaactga agaaagagac
     4261 aggcaagccc ctgaaacaag cggtgctcga cgacgcattt tcgcggatca aggtaacgta
     4321 cgatccgatt cgctcttcac tcgtcacctc cacgcaggaa gcgtttgaag caggattttt
     4381 gggccacaag cagccggaca tttccgggct gtacgacctg acgctgttga atgaagtgct
     4441 acgcgaaaag aaggcgcaac cactacaatg acccgatacg ttatagactt attcaatcgt
     4501 tcgataggcg gaggcctggt gcgcgctgaa aacgacgagc tgccggagcc gttgaaggtg
     4561 gagaaaaaat cctcctccga atctcgtccg aaaagcctgc cggagcactc ggccaaagag
     4621 agcatcgagc tgagcggagt tttccgcagc tacaagaatt cgcgcggctc attcactccc
     4681 gcgctgcaaa atatcgatct ggaaatcgag cagggagaat tcgtctgcat cgtcgggccg
     4741 tccggctgcg gaaaatccac cctgctgcat ttaatcgcgg gattggataa acctaccacg
     4801 gggcaaatta ccgttgacgg tggtcccgtc acaggtccgg gcacagatcg cattttactt
     4861 ttccaggagt tgggactttt tccgtggctc aatgtccggc aaaacgtgga attcggttta
     4921 aaaatggcgg gcgtgtcaaa cgaagagaga aaagagcgcg cacgaatttt tctgcgcatg
     4981 gttcacctct cccacttcga ggaccattac attcaccagt tgtccggagg catgaggcag
     5041 cgcgtggcgc tcgcccgctc gcttgcgctt cgtcctaaaa ttttattgat ggacgaacct
     5101 tttgccgcgc tcgacgcgca aacgcgggac atgctgcacg acgagctgga gcgcatctgg
     5161 aaagagacgg cgcctaccat tgtcttcgtg acgcacaacg ttcgcgaagc cgtgcgcctc
     5221 ggagaccgcg tcctgctgat gagtttccga ccgggaagaa ttaaggcgca atttgaagtg
     5281 agcctagacc ggcccaggca tgttgaggat tcagacgtgg ctcaactgtc aaaagaaatc
     5341 ctcgggcaat tgcgcgaaga aattgatcga tcgtttaatg ccgaatattc gcgagaagaa
     5401 aagcgttgag taggtagttt tttgattttt gtagtggggc attgtagggc acggcatgcc
     5461 gtgcacctgc ggtgcatgca cagctatagc gacgaaccga tcagctccac taaaatttca
     5521 ttgctgccgg cgtagatgcg ctgcacgcgc ccgtccatcc acatgcgcgc aatgggatat
     5581 tccgacatat agccgtaacc cccgaacatc tgaacgcatt cgtccagaat gcgactctgg
     5641 cattccgtcg tccagacttt caccatggcg gcggtcactg gatccatctt gccggctaaa
     5701 aattgcatga cgcagttgtc gacgtacacg cggccgattt gcgcttccgt cttggcgtgt
     5761 gccagcttga agcgagcgtt ctgaaattcc atcagcgtct gtccaaacgc tttccgctcc
     5821 tttgcgtaat ggatcgtgag ttccagcgcc cgctcgatcg cggcaatggc gccaacgctg
     5881 atcatcagac gttcgtagcg cagctgatcc atcatctgcg caaatcccgc gccttcgctt
     5941 tttcccagta aattggccgc agggacgcgc acatcatcaa agaaaagttc gcacgtatcg
     6001 atttcgtgca tgcccacttt ttccagcgat ttgccgacgc ggtatcccgg aagatttttc
     6061 gtctcggcca taatcatgga aatgccgcga aaaccagcta ccttcggatt ggtttttgca
     6121 gcgacgcaca cgaggttggc gacgccgcca ttcgtgatga aggttttgga accgctgatc
     6181 acgtactcgt ttccatcgcg tcgcgcggtt gtcttgatgg cttgcaaatc agagccagcc
     6241 gagggttctg tcatggcgat ggctccaatc agttccccct gcgccattgc cggaagccac
     6301 tttttctttt gctcttcatt tccgt
//
LOCUS       DASZMB010000093        16008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3649607, whole genome shotgun sequence.
ACCESSION   DASZMB010000093 DASZMB010000000
VERSION     DASZMB010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 16008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16008
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3649607"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VG272_04275"
     CDS             <1..1033
                     /locus_tag="VG272_04275"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWF12929.1"
                     /translation="GWTGVDLFFVLSGFLIGGILLDARASSNYFKTFYARRFFRIIPL
                     YYAWILLYVILAPLIRTFFSESIGPIQRVDGSILAHLFFLQNFHEFLKTGASFWWFSS
                     TWSLAVEEQFYLIAPLLVRYLSKRSLATVLVLVTLGAPMLRFAVRNYCSEGPWLAYRL
                     MPCRADALALGMLAALIWNSPKGHEWLERNSLILYGMFTFLFYGIAYLWRWSSDPMLL
                     LTQTAGYTFIALFFAILMLLVLTERAFLVSAVMRFGFLRELGGISYCVYIIHTAVFFF
                     SHQLILHALPAVTDVRAALVTFLAALLTYGIAKLSWKYVEQPQLRRGHAFRYDFVKPK
                     YLQPTESVT"
     gene            1108..3114
                     /gene="asnB"
                     /locus_tag="VG272_04280"
     CDS             1108..3114
                     /gene="asnB"
                     /locus_tag="VG272_04280"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HWF12930.1"
                     /translation="MCGICGVIGIQRTEPAEQMTRRMMGALTHRGPDEDGLLLAPSAA
                     LGMRRLSIIDLPGGHQPVFNESRNVAVVFNGEIYNFPQLRKTLEARGHMFRTASDTEV
                     IVHAYEEWGELCLRELRGMFAIAIWDARSSGTSGEAARHAKIFLARDRLGIKPLYYAV
                     AGGALLFSSEVRSLLASGQIPPRVSPDSLEAFLTFGSVIEPSTLVEDIFSIPPGHCIS
                     FSAYAPPTKPSPKPYWVYSDAVLQHDGPGPKDFNQAARQLRPLLEETVQDHLIADVPL
                     GIFLSSGLDSTSLVALASRFKSDLHTFTVVFPEQRYSEAAVSRETAKKFKTQHQEILL
                     APDALVGQLGDAVKSLDQPTMDGLNTYFVSRAAAQSGLKVALSGLGSDEIFGGYSTFE
                     STPRAAFVAGLGRWIPGPFRRLTAGAAVRIAAGDAVRKAAAAWRSPTDFPHAYYFTRL
                     LFTPSRVRRLLAPYFESKEYSGNHNNPWRARMAETSRQAAKLDSFTGVSCFELQSYMV
                     NTLLRDTDSASMANSLEVRVPFLDHRLVEFVGRLPKTTKYNRDVPKSLLVEALSDLLP
                     DEVVGQSKRTFTLPWEVWLRGPLGVRLSQDLANLTPPLRKYLNPRAVRGAWQNFVIGH
                     TTWSRPWSLYVLNEWIRHHVTDAADSSASTANTVEAAAASNPRS"
     gene            3136..4908
                     /locus_tag="VG272_04285"
     CDS             3136..4908
                     /locus_tag="VG272_04285"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HWF12931.1"
                     /translation="MYILGINAYHGNASAAIIADGRLIAAVEEERFNRVKYAAGFPAA
                     AIRYCLDTAGITLGEVNHIAIPRNPWARLGTKLLYALRMPSFATERAKVLTQFVGIPE
                     ALAKAFDVSPDSIRAKFHRVEHHQAHLASTFYVSPFDQAALLSADGLGDFASTMWGVG
                     RGDHMHIDGAIAFPHSLGLYYTAMSQYLGFRKFGDEYKVMGLAAYGEPAYMDEYRRIV
                     RNTRGIGFHLGLKYFKHHRTGPEMTWRDSDKTPELGAMYSDYMAKRLGPARDPSAPVE
                     KKHRDGAASLQARLEEVLFQMLRALHARTKQKAVCLAGGVAFNCVANGKIVDQTPFKK
                     IFVQPAAGDAGLAIGAAYFVHHQILGQPRTFVMEHSYWGPGYTHEKIAAAIKASPLQS
                     EGLVISEHHEKEVAIEAAKEVADGKILGWFQGRAEWGPRALGNRSIVADPRRLDMKET
                     LNARIKHREMFRPFAPSILAESTGEYFEKSDPCPFMTQAYSVRPEKRDVIPAPTHVDG
                     TGRLQTVTYQENPRYWRLIREFANLTGVPVVLNTSFNDNEPIVCRPEEAIDCFRRTKM
                     DVLVLGDFLIRKPKPPEAPRWTGE"
     gene            4970..6166
                     /locus_tag="VG272_04290"
     CDS             4970..6166
                     /locus_tag="VG272_04290"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWF12932.1"
                     /translation="MHILLLNEYFPPDTSATAKCAVQVVEALSANHRVTVLAGRPSYD
                     PTENHRPYFLRREVRGNLAIERVGSTAYPRFQMRRRVSNYLTYLSLAIPRALSIPSDI
                     VLAMTDPPIEGIAGAIVARLSGRPFVYNIRDMYPDMAVGGSIVRPGSFTARWETMHRW
                     ALRQAARVIVLGEDMRDRIIAKGIDPARVVVSRDGIVIPETVVSPDNPVAREIRGNFR
                     FVLVHAGNLGFYGAWQTLISAVRMLESEGVGLVFIGEGAMKPQIEAMAQGCNAIRFLP
                     FRPAGEIPQVLSSGDMHVVTVKRGLEGVVVPSKLYPTLAAGRPVLGIAPEECDVVRTI
                     RASGCGIAANPDDPASVADAIRSVLHNSEQLRTMGQRAREISFSYDRVKQLKIFSETL
                     EELARG"
     gene            6462..7505
                     /locus_tag="VG272_04295"
     CDS             6462..7505
                     /locus_tag="VG272_04295"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HWF12933.1"
                     /translation="MNTSLIAFAVGLIASMGLTVPVRQLALRVGMVDHPGPRKVHVQP
                     MPLLGGLAIYLGVLIALLVSMDGPALTQVIGIYAGATLVAVVGILDDRGLLHHQIKLF
                     GAMPAAAIILLISGIRAQVFSNIWPGMPGVVLDTALTLVWVVGITASFSILDHMDGLC
                     AGVAAMASLFFAIFALQSGQLMVSTMAAAVLGAAAGFLRWNFKPAKIFMGDGGAMFLG
                     FLMATLSLKLRPVIAAGHNAAWLIPIFILGATIFDTTLISISRSRRGFIPFTTPGKDH
                     TAHRLSNVFESQRTAVLLMYTVGAFFGVLALIISRLSAVPAYTVAALAAVVVLGAVAL
                     LERAPYERQTRKK"
     gene            complement(7645..8658)
                     /locus_tag="VG272_04300"
     CDS             complement(7645..8658)
                     /locus_tag="VG272_04300"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF12934.1"
                     /translation="MKLNVGLIGLGRLGKVYARDLAARVTGVRLAALADTNAKLAEET
                     ARELDVPRWYPDPIAMMDDPGIDAIIIVSPTDTHTELAVAALEHKKSVFCEKPPAISL
                     SQTQKMKDAAARTGGFLQMGFMRRFDAGYAAAKKKVEEGVIGKPIVFKSSSRDPYRPS
                     LEYANPKSSGGMIMDMGIHDFDLARWYMGEAESVTAIGGTLAYPELNEVGDIDNAIVT
                     IRFASGCIGVVDLSRSGIYGYDIFGELLGTTGTLRIGYLRETPLFVMTKNHIAHDTVP
                     FFMERFRDAYPTQLQNFVDNLRDNRQAPITIDDAMGSLRIALAATRAQATGETVLLSD
                     TSL"
     gene            9155..10402
                     /locus_tag="VG272_04305"
     CDS             9155..10402
                     /locus_tag="VG272_04305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWF12935.1"
                     /translation="MSMRQWRATGGIWERGKVTLQQGIKKGLGHKTGPQVAVIGGSAS
                     GFYTASLLARAGCPVEVLERADQLDPTPRTLIVTSHMRTLLGKIGETAVVNEIRKFEL
                     FTDGRSAQVPLDRPDLIVERSRLIRDLANDAQSNGAKVSFGNRFMGLQSNGTGLRVSV
                     ERAQSGCAEELHADVVVGADGALSRVAQSAGWPRQPTVPLVQAIVRVPADMSQHTVRV
                     WFVPDDTPYFYWLIPESSERAVLGLIGEDGQETRRCLERFMEKRGFTPLGYQGAKIPV
                     YDRWTPVERRVGAGRVFLVGDAAGQVKVSTVGGIVTGFRGAIGVSESIVNGGESSELR
                     SLRKELDLHLLIRKTIHQFKQADYSQLVDLMNDSTRRSLSQYTRDEASRILWSIALRQ
                     PRILLLALRGLFSGGSILPKHRA"
     gene            10798..12174
                     /locus_tag="VG272_04310"
     CDS             10798..12174
                     /locus_tag="VG272_04310"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HWF12936.1"
                     /translation="MKLAVIGCGYVGLVSGSCLAEAGHEVIATDNDGARIAALQAGKI
                     PIYEPNLDKVLEGAVRDGRLRFTADAGEAVRESDVIFICVGTPPTETGEADLSAIDNV
                     ARMIAKESRTPKLVIEKSTVPAQTGEQLKKALQVYATNSGAKFRVASNPEFLREGTAV
                     EDFFHPDRIVIGVEEKESEQQLRELYRPILEGRFRCPVHQPNCPPVATPDFVVTTIAS
                     AELIKHASNSFLGLKISYANVLADLCERLGGNVEEVTRAVGLDPRIGGQFLKAGLGFG
                     GFCLPKDIQAFIKLADRAGVDFGLLKEAELVNKQRVDRLLEKVKKALWVMKGKQIGVL
                     GIAFKPNTDDIRLAPAIEVIRRLLAEGSQVRATDPEAMERARAVFPNICYSADPYEIV
                     RGADALLVLTEWEQYRRLDWKRVRKEMARPLVIDGRNMLRPAEMRKLGFEYMSFGRPD
                     TVAVSAGQ"
     gene            complement(12195..13145)
                     /locus_tag="VG272_04315"
     CDS             complement(12195..13145)
                     /locus_tag="VG272_04315"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HWF12937.1"
                     /translation="MRAVVTGGAGFLGSHLCEYLLNRGWEILAIDNLVTGTPVNLRHF
                     PKNAPFAIIQHDVSKFIDVPGPVDYVLHFASPASPVDYLKLPIQTLKVGSLGTHNTLG
                     LALAKKAKYFLASTSECYGDPHVSPQPETYWGNVNTVGPRGVYDEAKRFAEAMTMAYH
                     RAKGLDTHIVRIFNTYGPRMRLNDGRALPNFVHQALTGQPITVYGTGEQTRSFCYVSD
                     LIEGIYRLMMSDEHEPTNIGNPHEITILEFAERVRSLMAVSTPIIFEPLPQDDPKQRC
                     PDISKARRVLGWEPKINLEEGLKLTLESFKKQFAETQVGS"
     gene            13672..14319
                     /locus_tag="VG272_04320"
     CDS             13672..14319
                     /locus_tag="VG272_04320"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HWF12938.1"
                     /translation="MTELKFGKRRWMIGGGIFAAIAILAATVFFTIGRWLVVEDPLSH
                     ADVIVVLSGRLPERAMEAAQVYKAGYADQIWISPPISPVDELKSMNISYLGEDFYNEK
                     VLIAQGVPPDAIRILDRPDANTEAEVRQTVEELRALNYHSVIIVTSSPHTRRVRTIWR
                     KLVGSDPHMMVHSAQDDPYDGAHWWRHTVDALDVVRETLGLLNAWAGFPLRPAQN"
     gene            14322..15893
                     /locus_tag="VG272_04325"
     CDS             14322..15893
                     /locus_tag="VG272_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12939.1"
                     /translation="MPSRQRKPSELTQQVIPLLLLAIGLMTTNNSAPVIDDEAIILGG
                     AASPLRSSLASFFSGAGQHEHPPLYDVLLHFWLRWTQGNFDFLRIPSILFFIAGLFLL
                     ARATRHFTGPKGGYAVIWIGVLWPFGFHFARLAAWYSFSFFLVAGLTLSYFNYLQEQT
                     VGRWAALFLFSAVLLWTNYFGWAILACLALDQIIRWRSKEPAASPKVLLGTAALLVAI
                     FVPLISPFRVELSHGIHLHNGPVSMIANMAFNVFSLFVSESVAPWFWWLSIPVGVAIL
                     SCLILVGWWLVRPARRLLLYGALLIVAMALIGILQTKYLLMISPWVLLPAGIAIESAK
                     PRWATFGLAGALLLIGAAGWYGIYSKRYYSAPRFVEPWQEIAADAANQINSGATVIAD
                     HPSFLFYLTYYLRVPAQNGPWRFEGLLPDSVTHPQVFSPHGWLSAGHPTRGKMMVIRA
                     GRDLDGTDPIDNAIKLLDQTCGSISSRLRMRDTGYAWKQRFFPNLGEPLWRIEIREYD
                     CNSTNSKQIYPLPSQ"
BASE COUNT         3294 a         4268 c         4519 g         3927 t
ORIGIN      
        1 ggggtggaca ggggttgatc tgttctttgt tctctccggc tttctgattg gcggaattct
       61 gctggatgct cgagcctctt ccaattattt taaaacgttt tacgcgcgca gattttttcg
      121 cattattcct ctgtattacg catggattct tctctacgtc attctcgctc cgctgatcag
      181 gacatttttt tcggaaagca tcggacccat tcagcgggtt gatggctcca tcctggcgca
      241 tttgtttttc ttgcagaact ttcacgaatt tctaaagacc ggcgcatcgt tctggtggtt
      301 ttcgtccacg tggtcgctgg ccgtggaaga gcagttttat cttattgcgc cgctgctggt
      361 gcggtacctg tccaaacgtt cgcttgcaac tgttctggtg ctggtcacgc tcggagctcc
      421 gatgctgcgg tttgcagtgc gcaactattg ttcagagggg ccgtggctcg cataccgcct
      481 gatgccgtgc cgcgcggatg cgctggcctt gggcatgctc gcagcgttga tctggaatag
      541 tcctaagggg catgaatggc tggagcggaa ctcgcttatt ctgtacggga tgtttacgtt
      601 tcttttttac ggaatcgcct acctgtggag atggtcctcg gacccgatgc ttcttctgac
      661 gcaaacggcc ggatacacgt tcatcgcgct cttcttcgcg attctaatgc ttctggtgct
      721 cacggagcgt gcattccttg tgtccgccgt gatgaggttt gggttcttgc gtgagcttgg
      781 cggcatttcg tactgcgttt atatcattca cacggcggta ttttttttca gtcaccagct
      841 tattttgcac gcgcttcccg ctgtgaccga tgtgagggct gcgctcgtaa cgttcctcgc
      901 cgccttgctg acttatggca tcgccaaact atcctggaag tatgtcgagc agccacaact
      961 acgccgcggg catgcctttc gatatgattt tgttaagccc aaatacttgc agcccacgga
     1021 aagtgtcact tgattttgta acctggtagg caacattctt caggtaagag tggaactgcg
     1081 aacgcgttca cggtaaagta acgcggaatg tgcggaatat gcggtgtcat aggaattcaa
     1141 cgaaccgagc ccgccgagca aatgactcgc cggatgatgg gggcgctcac gcatcgcggc
     1201 ccggacgaag acgggcttct tctggctccg tccgctgctc tcggcatgcg ccgcctcagt
     1261 attattgacc tgcccggcgg gcaccaacct gttttcaacg aatcccgaaa cgttgccgtc
     1321 gttttcaacg gcgaaattta taattttccg caattgcgga agacactcga agcccgcggc
     1381 catatgttcc gaaccgcttc ggataccgaa gtcattgtcc acgcgtatga ggaatggggt
     1441 gagctgtgcc ttcgcgagtt gcgcggaatg tttgccatcg ccatctggga tgcgcgttcc
     1501 tccggcactt cgggagaagc ggcgcggcat gcgaaaatat ttctggcacg tgaccggctc
     1561 ggaatcaagc cgctatatta tgctgtcgcg ggcggcgcat tgttattttc atccgaggtg
     1621 cgctcgcttc tggccagcgg ccagattccg ccgcgtgttt cgccggattc tcttgaagca
     1681 ttccttacgt ttggctccgt gattgagccg tccactttgg tcgaggatat tttctcaatt
     1741 cctcccggcc actgcatttc gttttccgcg tatgctccgc caacaaagcc ctcgccgaaa
     1801 ccctattggg tttattcgga tgccgtactt cagcacgatg gaccgggccc caaggatttc
     1861 aatcaggcgg ccaggcaatt gcgcccttta ctcgaggaaa ccgttcaaga ccatctgatc
     1921 gccgatgttc ctctcggaat atttctttcc agcggtctgg actccacaag cctggtggct
     1981 ttggccagcc gatttaaaag tgacctgcac acttttaccg tggtgtttcc ggagcagcga
     2041 tatagcgaag ccgcggtttc gcgcgaaacc gctaagaaat tcaagaccca gcatcaggaa
     2101 attcttttgg caccggacgc tcttgtcggc caattggggg atgcggtcaa atccctggac
     2161 cagccgacca tggatggact gaatacgtat ttcgtttcac gtgccgcagc ccagagcgga
     2221 ttaaaggtgg ctctatcagg gcttgggagc gatgaaatct ttggcggata ttcgacattc
     2281 gaatcgactc ctcgcgccgc attcgttgcc ggacttggcc ggtggattcc ggggccgttc
     2341 cggcggctga ctgcgggcgc tgcggtaagg atcgccgcgg gagatgcggt tcgaaaggcc
     2401 gcagcggcat ggcgttcgcc cacggatttt cctcatgcgt attacttcac gcggctgctg
     2461 tttactccct ctcgtgtgcg gcgtttgctt gctccctatt tcgaatccaa ggagtattcg
     2521 ggcaatcata ataatccgtg gcgggcgcga atggcggaaa cctcaaggca ggctgcaaag
     2581 ctggattcct tcaccggcgt ttcatgtttc gaactgcagt cctacatggt gaatacactg
     2641 ctgcgggaca cggatagcgc gagcatggcc aattcgctcg aagtgcgcgt gccattttta
     2701 gatcaccgtt tggtggaatt cgtgggccgg ctgccgaaga ccacaaaata caatcgagat
     2761 gttccaaaat ccctgcttgt cgaggcgctt tcggacttat tgcccgatga agtggtcggc
     2821 caatccaaac gaacatttac gctcccctgg gaagtgtggc tcagagggcc gcttggcgtg
     2881 cgtttatcgc aggatctcgc gaatcttact ccgccattgc gcaagtatct gaatccgcgc
     2941 gcggtgcgag gtgcttggca gaatttcgtg atcggccata caacgtggtc gcgtccctgg
     3001 agtttatatg tgctgaatga gtggattcgc catcatgtga cggatgccgc ggacagttcg
     3061 gcttcaaccg cgaatacagt tgaagctgcg gcagcctcaa acccgcgatc ataatggaag
     3121 ggaaaccgat tctggatgta tatccttggg attaacgcgt atcacggcaa tgcctctgcc
     3181 gcaatcatcg cagacggcag actgattgcc gcggtggaag aagagcgttt caaccgtgtg
     3241 aaatatgcgg ctggttttcc ggctgccgcc attcgatatt gcctggacac cgcgggaatc
     3301 acgttgggcg aagtgaacca catcgcgatt ccccgaaatc cctgggcacg cctcggaacg
     3361 aaattgttgt acgcgctcag gatgcccagt tttgcaaccg agcgggcgaa agtcttgacg
     3421 cagtttgtgg gaatccccga agcgcttgcc aaggcgttcg atgtttcgcc ggattccatt
     3481 cgagcaaagt ttcatcgcgt ggaacatcat caagcgcacc tcgccagcac tttttatgtt
     3541 tcccccttcg atcaggccgc gctgctgtct gccgatgggc tgggcgattt cgccagcacc
     3601 atgtggggag ttggccgcgg cgatcacatg cacatcgacg gcgcgatcgc ttttccgcac
     3661 tcgcttggcc tgtattacac ggccatgtct caatacctgg ggtttcgtaa attcggcgac
     3721 gaatacaaag tgatgggcct cgccgcatac ggcgagcctg cttacatgga cgagtaccgg
     3781 cgcattgtcc ggaacacgcg agggatcggc ttccacctgg gactcaaata tttcaagcat
     3841 caccgcaccg ggccggaaat gacctggcgc gactcagata aaactcccga actgggcgcg
     3901 atgtattcgg actatatggc caagcggctt ggtcccgcgc gcgacccttc tgctcccgtc
     3961 gagaaaaaac accgggacgg cgcggcgagt ctgcaagcgc gattggaaga agttctgttt
     4021 caaatgttgc gtgcgctgca cgctcgcacc aaacaaaaag ccgtgtgcct ggctggcgga
     4081 gttgcattca attgcgtggc caatggaaaa attgttgacc agacgccatt taaaaaaatt
     4141 tttgtgcagc ccgcggcggg tgacgctggt ttagccatcg gcgctgctta ttttgtccac
     4201 caccagattc ttgggcagcc acgtacgttt gttatggaac attcgtactg gggaccgggc
     4261 tacacgcacg aaaaaattgc cgccgcgatc aaggccagcc ccctgcaaag cgaagggttg
     4321 gtcatctcag agcatcacga aaaagaagtt gcgattgaag cggcaaaaga agtcgccgat
     4381 gggaaaattc ttggctggtt tcaaggccga gccgaatggg gccctcgagc cctgggaaat
     4441 cgcagcatcg ttgcggatcc gcgcaggctg gatatgaagg aaactctgaa tgccagaatc
     4501 aagcacaggg aaatgttccg gccctttgct ccctcgattc ttgcggagtc cacgggagaa
     4561 tatttcgaga agtccgatcc gtgcccgttc atgacgcaag cctattccgt gcgtccggaa
     4621 aaacgcgacg tgattcccgc gcccacgcac gtggatggaa caggccgcct gcaaaccgta
     4681 acgtatcagg aaaacccgcg ctattggcgg ctgattcgag agtttgcgaa tcttaccggc
     4741 gtgcccgtgg tgctgaatac gtcgttcaac gacaatgaac caatcgtttg ccgtcctgag
     4801 gaagcgattg attgttttcg acgcaccaaa atggatgtgc tggttctggg cgacttcctg
     4861 atccgcaaac ctaaacctcc cgaggcgccg cgctggactg gcgaatagcg atcttgcaag
     4921 gatttgattt cggtgctgcc ccaatctcta cttatccccg gataagcccg tgcatattct
     4981 tctcttaaac gaatattttc cgccggatac gagcgcaacg gcaaagtgtg cggtgcaagt
     5041 tgtcgaagcc ctctctgcaa atcaccgcgt aactgttctg gcgggccgtc caagttatga
     5101 tccgaccgaa aatcatcgcc cttatttttt gcggcgcgaa gttcgcggca atctggccat
     5161 cgagcgcgtt ggctcaaccg catatcctcg ctttcaaatg cgccgccggg tttcaaatta
     5221 tttgacgtat ctttcgctgg caattccgag ggcgctgtcc attccttcgg acattgtgct
     5281 ggccatgacg gatccgccga ttgaaggcat cgccggtgcg atcgttgccc gtctgtctgg
     5341 gcgtccgttt gtctacaaca ttcgcgacat gtatccggac atggccgtgg gcggctcgat
     5401 tgtacggccc ggaagtttta ctgcgcgatg ggagacgatg catcgctggg cgctcaggca
     5461 agctgcgcgc gtcatcgtgt tgggtgaaga tatgcgcgac cgcatcatcg cgaaaggaat
     5521 cgatccggct cgcgtggtcg tctcccgcga cggaatcgtg attccggaga ctgtggtttc
     5581 gcctgacaat ccagtggctc gggaaattcg cggcaatttt cggttcgtgt tagtccacgc
     5641 cgggaatttg ggtttctacg gagcttggca aacgctgatt tcagctgtgc gaatgctgga
     5701 aagcgaaggc gtgggtttag tgttcattgg cgagggcgca atgaagccgc aaatagaagc
     5761 catggcgcag ggttgcaatg ccattcgctt cctgccattc cgccccgcag gcgaaattcc
     5821 gcaggtactc tcttccggag atatgcacgt cgtcaccgtg aagagaggtt tggagggagt
     5881 ggttgtgccc agcaaacttt atccgacgct ggcggccggg cggcctgtgc tgggaatcgc
     5941 tcccgaagaa tgcgacgtgg tgcgtacgat tcgcgcgagc ggttgcggaa tcgccgcgaa
     6001 cccggacgat cccgcgagtg tcgcggatgc tatccgcagc gtgttgcata actcggaaca
     6061 attgcggacg atggggcagc gggcgcgtga aatttctttt tcctatgata gagtaaaaca
     6121 gttgaagatt ttttccgaga ctctggaaga actggctcgc ggataaagaa gtatggcgaa
     6181 atgttccgtt ccttggatta ggaattgtct ggctttggaa gttgccgtga agttgaaagt
     6241 ttctgtggag ttggaagttg ccgtagggtt ggaagtcggg tgaagttgct ttggaggagg
     6301 tccgaccttc aggtcggaca atcagcatcg cgtaggaagg cctttaggcc ctgaagcttc
     6361 aggggctgaa gcccctgcta ctcgaagcga acgtcgaggc taaagccccg acctcctgaa
     6421 acgcaaagcg atcgcgacaa tttccaagtc caggacagct catgaacacg agcctgattg
     6481 cattcgccgt tggcctgatt gcctcgatgg ggctcaccgt tcctgtgcgc caactggctt
     6541 tgcgcgtggg aatggtggat catcccggcc cgcgcaaagt gcacgtgcaa cccatgccgc
     6601 tgcttggcgg tttggcaatt tatttaggcg tgctgatcgc gctgctagtc tccatggatg
     6661 gccccgcgct gacgcaggta attgggattt acgcgggcgc aacgcttgtc gctgtagtgg
     6721 gcattttgga tgaccgcggc ttgcttcatc atcaaattaa attgtttgga gctatgcccg
     6781 ccgccgccat tattctgctt atctccggca tccgcgcgca ggttttctca aatatttggc
     6841 cgggcatgcc gggagttgtg ctggacacgg cgctgaccct ggtctgggtc gtcggcatca
     6901 ccgcttcctt cagcattctt gatcacatgg atgggctttg cgcgggcgtc gccgcgatgg
     6961 catcgctctt cttcgctatt ttcgcgctgc aaagtgggca gctgatggtc agcacgatgg
     7021 ccgccgccgt cctcggagcc gccgcaggtt tccttcgttg gaatttcaag cccgcgaaaa
     7081 tttttatggg cgatggcgga gcaatgtttc tcggcttcct gatggccacg ctctctctca
     7141 agctgaggcc ggtgattgca gcgggtcaca acgccgcctg gctgatcccc attttcattc
     7201 tcggcgcgac gatctttgac acgactctaa tttccatttc ccgttcccgc cgcggattca
     7261 ttccattcac gacgccgggc aaggaccaca ccgcgcaccg cctctccaac gttttcgaaa
     7321 gccagcgcac tgcagtattg ctcatgtata cagttggcgc atttttcggt gtgttggccc
     7381 tgatcatcag ccgcctgtcc gccgtgccag catacacagt tgcagcgctc gccgccgtcg
     7441 ttgtgctcgg cgcagtcgcc ctactcgaac gcgcccccta cgaacgccaa acccgcaaga
     7501 agtaactgaa ttgttctcca ggatttaaat tccagcagac tggcaataga aaatcccacc
     7561 cttcgctttg tcagaaaaat ggatatgtaa aatgcgacgt tgtgtttttg actgaaaaaa
     7621 gccaaatgca ggtagcgctt gttcctacaa ggatgtgtcc gacaacaaca cagtttcccc
     7681 cgtagcctgc gcgcgcgtcg ccgcgagcgc tatgcgcagc gaacccatcg catcgtcaat
     7741 ggtgatcggc gcctggcgat tatcgcgcag attgtctacg aagttttgca gttgcgtggg
     7801 atacgcgtcg cggaaacgtt ccatgaaaaa aggaacggtg tcgtgcgcga tgtgattctt
     7861 cgtcatcacg aagagcggcg tctcgcgcag gtatccgatt cgcagcgtgc cggttgtgcc
     7921 aagaagttct ccaaaaatgt catagccgta aattccgctg cggcttaaat cgacgacgcc
     7981 gatgcagccg ctcgcaaatc ggatcgtgac gatggcgttg tcgatatcgc ccacttcatt
     8041 taactccgga taggcgagcg tgccgccgat ggccgtgacc gattctgctt cgcccatgta
     8101 ccagcgcgcc aaatcgaaat cgtgaatccc catatccata atcatgccgc cgctgctttt
     8161 gggattggca tattccaacg acgggcgata gggatcgcgt gaagacgatt taaaaacgat
     8221 gggtttgccg atgacgcctt cctccacttt cttcttggcg gcggcatagc cggcgtcaaa
     8281 gcggcgcatg aatcccattt gcagaaaacc acccgtgcgc gcggcggcat ctttcatttt
     8341 ttgcgtttgg ctgagcgata ttgccggagg tttctcgcaa aacacggact tcttgtgttc
     8401 cagagcggcg acggcgagtt cagtatgcgt gtcggtgggg ctgacgataa tgatcgcgtc
     8461 aatgcccggg tcgtccatca tggcgatggg atctggatac cagcgcggca cgtccagctc
     8521 acgcgcggtt tcctccgcga gtttcgcatt cgtgtccgcc aacgccgcaa ggcgcactcc
     8581 cgtgacgcgc gcagccaaat cacgcgcata tactttccca agccgcccca gcccaatcaa
     8641 gcccacattc aacttcattg cttccccctg atatgttttt tccgcgtcga aatattccgt
     8701 tttcttcgcg tctactataa gcgaaacaaa ataatccgca ttttaaatta cggttcgcgc
     8761 atggtacgct tccgctgctt tcgaggcaat tcatttgctt ccgccaattt cctattctgg
     8821 cgacccttca gaagaacaaa aaaggcatcg ctgtgaacac ccgcctcgat ttcactcatt
     8881 cataagatat gttgaaaagc agcactctcc ggtccgttaa tcccaaaatg gagggacgag
     8941 agtttcttcg ggcgggacgt cgccttccgc tttgccttat gtcgcggttt ggctatactg
     9001 ctctgtcacg attttacgtt ggcgatggga actctgcgtg ggtaagcggt tacttgactg
     9061 ctcctatcgc gtggtcgggg agcgctagcg gccgtccttg gacgttaggc ctcataattc
     9121 gtcctcagtc agatcggaaa tagaaagcgc ttccatgtcg atgcgccaat ggcgtgcgac
     9181 aggtggtata tgggagcggg ggaaggtcac actgcagcaa ggaattaaaa aagggctagg
     9241 ccacaaaacc ggaccgcaag tcgccgtcat tggcggttca gcttccggtt tttatacagc
     9301 atcgcttttg gcacgggcgg gttgccctgt ggaagtgctg gagcgggctg accagcttga
     9361 ccccacgccg cgcactctga tcgttaccag ccacatgcgc accttgcttg gcaaaatcgg
     9421 cgagaccgcg gtcgttaatg aaatccgcaa atttgaatta ttcaccgatg gccgaagcgc
     9481 ccaggttcca ctggatcgtc ccgatttgat tgtggagcgg tctcgcctga ttcgcgatct
     9541 tgcgaacgat gcgcaaagta acggcgcaaa agtttctttc ggcaatcgct tcatgggtct
     9601 gcaaagcaat ggcaccgggc ttcgcgtgag cgttgagcgc gcacaaagtg ggtgcgcgga
     9661 agaactgcac gcggatgtgg ttgtgggcgc cgatggcgcg ctttcgcgtg ttgcgcagag
     9721 cgctggctgg ccccggcagc cgacggtacc tctggtgcag gcgattgtgc gcgtgcctgc
     9781 ggacatgtca cagcacacag tgcgcgtctg gttcgtgccg gacgacacgc catactttta
     9841 ttggctgatt ccggaatcca gtgaacgcgc ggtgttgggt ttgattgggg aagacggcca
     9901 ggaaactcgc cgctgcctcg aacgctttat ggagaagcgc ggattcacgc cgctcggata
     9961 ccagggcgca aaaattcctg tctatgaccg gtggacgccg gtggaacgac gcgtgggcgc
    10021 gggccgcgta tttctggttg gcgacgctgc cggacaagta aaagtttcaa ctgtgggcgg
    10081 gatcgtcaca ggatttcgtg gcgcaatcgg cgtcagcgaa tccattgtga atggcggaga
    10141 aagcagcgag ctgcgttcgc tgcgcaaaga actcgacctt cacttgctga tccgcaaaac
    10201 gattcaccaa ttcaagcagg ccgattacag ccaacttgtg gatttgatga acgattccac
    10261 gcggcgctcg ctcagccaat atactcgcga tgaagcttcg cgcatccttt ggagcatcgc
    10321 cctgcggcag ccgcgaatct tgttgctggc tcttcgtgga ttgttttccg gcggctccat
    10381 cctccccaag catcgcgcct gaatttcctg aaggagaaaa gtttttagta ggacaggctt
    10441 cagagtctgt gtgagaactg cagaatcttt gtgggtcgtg gctttagcca cgacataagt
    10501 cctgcaaaat gagtgcggct ttagccgctg aagcttcaat ttttgcagtt cacacaggct
    10561 cttcagcctt cttcttttga tttttcttcg gtgccgcgga attcaaaagc ggacagactg
    10621 aagccggtgc tacgtgactt ttcctcacca atagaatctg tcaaatttac atttttaaaa
    10681 ccgtcgtgtt ggcacagcaa ctttaattag aaagttgctt ttgttcgctg ggagaatcgg
    10741 cgagaatctg ttcgcacgga tgttaaaagc ggctatgcgc tttaagggag aattagcgtg
    10801 aaactggcgg tcattggctg cgggtatgtt gggctcgtgt cgggaagttg tctggcggaa
    10861 gcggggcatg aagtcatcgc gacggataac gacggcgcgc gaatcgctgc gctgcaagca
    10921 ggaaaaattc caatttacga gccgaacctg gacaaggtgc tggaaggcgc cgtgcgcgac
    10981 ggccgattgc gctttaccgc cgacgccggc gaggccgtgc gcgagtcaga cgttattttt
    11041 atttgcgtgg gaactccgcc aaccgaaacg ggcgaggcag acctttcggc gatcgacaac
    11101 gtcgcgcgca tgatcgcaaa agaatcgcgt actccgaagc tggtgattga gaaaagcact
    11161 gtgcctgccc agaccggcga gcaactgaaa aaagctttgc aagtctatgc gaccaactcc
    11221 ggcgcaaagt ttcgcgtggc ctcaaatcct gaatttttgc gcgaaggaac ggccgtcgag
    11281 gacttctttc atcccgatcg catcgtgatc ggcgtcgagg aaaaggaaag cgagcagcag
    11341 ttacgtgagt tgtacaggcc gatcctggaa ggccgattcc ggtgtccggt acaccagccg
    11401 aattgtccgc ccgtggcgac tcccgatttt gttgtgacga ccattgcgag tgcggagctg
    11461 atcaagcacg cgtcaaattc attcctgggg ctgaaaattt cttacgcgaa tgtgctggcg
    11521 gatttgtgcg agcggctggg cggaaatgtg gaagaagtga cgcgagccgt gggcctcgat
    11581 ccgcgtatcg gcggtcagtt tctgaaagcg ggcctgggct tcggaggatt ttgcctgccg
    11641 aaagatattc aggcattcat caaacttgcg gatcgtgccg gcgtggattt tggcttgctc
    11701 aaggaagccg aactcgtgaa caagcagcgt gtggaccgcc tcctggaaaa agtgaagaag
    11761 gcgttgtggg tgatgaaggg aaaacagatt ggcgtgctcg gcattgcgtt caagccaaac
    11821 acggatgaca ttcgtttggc gcccgcgatc gaagtgattc gtcggcttct ggcggaaggt
    11881 tctcaagtgc gagcgacgga tcccgaagca atggaacgtg cccgagccgt gtttccgaat
    11941 atttgctact ccgcggaccc ctatgaaatt gtgcgaggag ccgatgcttt gctggtccta
    12001 accgagtggg aacaatatcg ccggctcgac tggaagcgcg ttcgaaaaga aatggcgcgc
    12061 ccgctggtga tcgacggccg gaatatgctg cgtccggcgg agatgcgaaa gcttggattc
    12121 gaatatatga gcttcggaag gccagacacg gtcgcggtaa gcgctgggca gtaagtagcc
    12181 taaactacga tctttcaact acccacttga gtttccgcaa actgcttttt gaaagactcc
    12241 agcgtcagct tcaaaccttc ttccaaatta atcttaggtt cccagccgag cacacgccgc
    12301 gctttggaaa tgtccgggca gcgctgtttc ggatcatctt ggggcaaagg ctcgaagata
    12361 ataggtgtgc tcacggccat caggctacga acgcgctcgg cgaattcaag aatggtaatt
    12421 tcgtgcggat ttccgatgtt tgtaggttca tgctcgtcgg acatcatcag gcggtaaata
    12481 ccctcgatga ggtcggagac atagcagaag ctgcgcgtct gctctcccgt gccgtaaacg
    12541 gtgatgggct gtccggttaa ggcttggtga acgaaattcg gcaacgcgcg gccgtcattc
    12601 aggcgcatgc gcggcccgta cgtattaaaa atgcggacga tatgtgtatc cagtcccttg
    12661 gcgcgatggt aggccatagt catggcttcg gcgaaacgct tggcttcgtc gtaaactccg
    12721 cgcgggccga ccgtgttcac atttccccaa taagtttccg gctgcggact gacgtggggg
    12781 tcgccgtaac attccgacgt ggacgcgaga aaatatttcg ctttcttcgc cagggccaat
    12841 cccagcgtgt tgtgcgtccc gagggaacca actttcaacg tttggatcgg aagcttcaga
    12901 tagtcgaccg gactcgccgg ggaagcaaag tgaaggacgt aatcgacagg gcccgggacg
    12961 tcgataaatt tgctgacgtc atgctgtatg attgcaaaag gagcattctt cggaaaatga
    13021 cggaggttaa ctggagtacc tgtaaccaga ttgtcgatcg cgagaatttc ccagccgcga
    13081 ttgaggaggt attcgcaaag gtgcgatccg agaaatccag cgccacccgt gactactgcg
    13141 cgcaaaggac caaccttcca ttgctgagat tttcctgacg attgattctc ttcatgatct
    13201 gctattccct cgaacagtcg aaaatcgtct gtctgctgaa tttcattgta tccgctgttg
    13261 tcctcactac aaagggcaac tcaatgcctg cagctccaag tcgcaaatac acgtagacgc
    13321 cttctcgatt gggttttttt aaatgaaata gcgatatttg acctccaaag gctgtcgggt
    13381 tacgatttca aagccgcccc taagacaatg tctcacggtc gattctattt gctgcgatgc
    13441 tgtccgtctt gcaagagtct tgacgttcgg cgttctcacc ggcgcggatt atttgaattg
    13501 ctcattcttc cgtttgtgtt gctgcggcct tttcgctgcg aggattgcac caagcgacac
    13561 tataatctgt tttttaccag ggctctccac gaaccggcgg aaggatctcg ccgaagtgct
    13621 tcaggagcgc gcggttagtt tcgtggtgca gccgggctac aaggcggaaa tttgacagag
    13681 ttaaaatttg ggaagcgccg ctggatgatt ggcggtggaa tttttgccgc gattgcaata
    13741 ctagctgcca cggttttctt taccattgga agatggctgg tcgtcgaaga tcctctttcg
    13801 cacgccgacg tcatcgttgt gttaagcggg cgccttcctg aacgcgccat ggaagcggcg
    13861 caggtctata aggccggata cgcggaccag atttggattt ctccgccgat ttcccccgtg
    13921 gatgaattaa agtcgatgaa tatttcctat ctgggcgaag atttttacaa tgaaaaagtt
    13981 ttgatcgctc agggagtccc gcctgacgcc attcgaatat tggaccgccc tgacgcgaac
    14041 acggaggccg aggtccgcca gactgtggag gaattgcgcg ctttgaatta ccattccgtg
    14101 attatcgtga catcaagtcc tcatacgcgc cgtgttcgga cgatctggcg caaattggtt
    14161 gggtccgatc cacacatgat ggtgcattct gcgcaagatg atccctacga tggcgcacac
    14221 tggtggcgcc acacggtgga tgctttggat gtggtacgag aaactttagg ccttctcaat
    14281 gcctgggcgg gattccctct gcgccccgcg cagaactgat catgccttcc cgccaacgga
    14341 aaccgagcga actcacgcag caggtgatcc cattgctgct gttggcaatc ggcctgatga
    14401 ccacaaataa ttccgcacct gtgattgatg acgaggccat catactgggc ggggctgcca
    14461 gcccgctccg ctcctcgctg gcctcatttt tctccggcgc tgggcagcat gaacatcctc
    14521 cgctgtacga cgtcctactt cacttctggc tgcggtggac gcagggaaat tttgactttc
    14581 ttcgcatacc ctccattcta ttttttattg ccgggctttt tctcctggcg cgcgccacac
    14641 gccatttcac gggaccgaag ggtggttatg cggttatctg gataggcgtc ctttggccat
    14701 tcggctttca ttttgcgcgc cttgctgcat ggtattcgtt ttcatttttt ctggttgcag
    14761 gtttaactct ctcctatttt aactatctgc aggaacaaac agtcggacga tgggccgcgc
    14821 tatttctgtt cagcgcggtt ttgttgtgga ccaattattt tggttgggcg atcctcgcgt
    14881 gcctcgcgct ggaccagata attcgttggc gaagcaaaga gccagcggca tctcccaagg
    14941 tgcttcttgg aacagcagcc cttctggtcg ccatttttgt accgctcatt tcgccatttc
    15001 gcgtggagct ttcgcacgga atccacttgc acaatggccc ggtttcgatg attgcaaata
    15061 tggcgtttaa cgtgttttcg cttttcgtca gcgaatccgt tgcgccgtgg ttctggtggt
    15121 tgagcatccc tgtcggggtg gcgattttat cctgcctgat tctggtcgga tggtggctag
    15181 tgcgccctgc gcggcgattg cttttgtatg gcgcattgct gatcgtcgcg atggctttga
    15241 ttgggatcct acaaacaaaa tatttgctta tgatttcgcc ctgggttttg cttcccgcgg
    15301 gaatcgcgat tgaatcggcg aagccgcgct gggctacctt cggattggcc ggagcgcttt
    15361 tattgattgg ggcggcgggc tggtatggca tctactcaaa acgatattat tccgcgccaa
    15421 gattcgttga gccatggcag gaaattgcgg ctgacgcagc caatcaaatt aacagcggcg
    15481 cgaccgtcat tgccgaccat ccctcctttc tgttttatct cacgtattat ctgcgcgttc
    15541 ctgctcaaaa cgggccgtgg agatttgaag gcttgctgcc tgatagcgtc acgcacccgc
    15601 aagttttttc tccgcatggc tggctgagcg ccggacatcc aacacgcgga aaaatgatgg
    15661 tgatccgggc cggccgtgat cttgatggga ccgacccaat tgacaatgca attaagctgc
    15721 ttgaccagac gtgcggatcc atatcgtctc gcctacgcat gcgcgacaca ggatatgcat
    15781 ggaaacagcg tttcttcccc aacctgggtg agcctctctg gaggattgaa attcgtgagt
    15841 acgattgcaa ctcgacgaat tcaaagcaga tctatccgct gccttctcaa tagccgtagg
    15901 gcttcaatca cctcttgcta ggcaattctt gcgtcaggat ttaccccaca aacttgtagt
    15961 acgtcaggtc cattgtcagc actcgcggaa tggttcaaac tggtagtg
//
LOCUS       DASZMB010000094        19296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_648318, whole genome shotgun sequence.
ACCESSION   DASZMB010000094 DASZMB010000000
VERSION     DASZMB010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 19296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19296
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648318"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1733)
                     /locus_tag="VG272_04330"
     CDS             complement(150..1733)
                     /locus_tag="VG272_04330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HWF12940.1"
                     /translation="MGTLSIWIAFNAGVAFFLLLDLALFHRRPHAITWREGALESAGW
                     IAVSLGFGAWIFISHGRTPGLEFFSGYLVEKSLSADNIFVFILIFQYFRVDPRYQHRL
                     LFWGVLGAIVLRGVMIGFGAVLIERFEWILYIFGAFLLYSGFKLFGADHSVHPEKNPV
                     MRWARKRLPMSKEGAGQKLFLRQSGKWLFTPLFLVVVVLETTDLVLAVDSIPAVFGVT
                     RDPFIVYSSNVCAILGLRALYFLLAGTLQYFQYLDEGLALTVMFIGAKMLAAPWVHIS
                     TGLSLTVVGGIIALAILISVIKAKMQMKDRPGKTYTDYTYAFNPVPVTPELIGHLADR
                     DPVERARTAERLFWTGISRVFISLRYWVEDKDFQTLLVRENYGSETPIVPFFASLTVG
                     IAVLPETFEKIRAANGSVPLADAPADQDVLEYELEFKKNILPHVRLDILTTKASGGNG
                     AIARFLDKFGEGIQQVEIDVTDVDRATEILRTRFNIPPIFPATRAGANGTRVNFFLVT
                     ASDNQKLLVELVEQPKQNP"
     gene            complement(1778..2650)
                     /locus_tag="VG272_04335"
     CDS             complement(1778..2650)
                     /locus_tag="VG272_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12941.1"
                     /translation="MKTKPYIHAVILLLAVLSFLLPARLAAQSTGRIEFEARVAATGG
                     QPEPVRQLPFYLLRKSLQDIRAEATQAAPAPDLDKFVDGLEVSPELKAWMKKHHSVQL
                     SGEEFTKSLTPEDIVGISEYFKAYMTHNAAYRGMGFPEPKFKEKDRKANPEKFQEQKD
                     QYEASVRKFIAASPDSVKGMDLELVDLNPIAKWVALENKHAKMVEANVMQIAEERYVV
                     ARAETDLDGRGSFSGVAPGNYWIGMFGAEAISGDVRQHWDLRVTLRQGETASIELSNF
                     NAIRSNTSAQNSSN"
     gene            complement(2935..4098)
                     /locus_tag="VG272_04340"
     CDS             complement(2935..4098)
                     /locus_tag="VG272_04340"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HWF12942.1"
                     /translation="MPKVVLPEGSTAPRLGGSQKPLSRGFVSNLKDFLTERPVKINKN
                     VRSDVFTVAEYGGGVAENFKEWFKPTPKAVSSRMTVDWQPSYKVLWQNLRDLISPPKL
                     PPLQVTSKPVRVKEIWTKDKAFGPSQAIALGVHVALTILVFSPFLYTVVTKATSNPVN
                     NSLVTPIDISDYTMKLPPGKDKAGGGGGGGERNPLPATKGKAPKFSFNQIAPPMVHTK
                     PESKIEATLLGPPDLKIPSPNMDNYGDPLAKLMNTSGGPGGGSGIGSGTGTGIGSGEG
                     GGLGPGSGGGTGGGAYHPGTGGVGFPSCVYCPDPKYSEEARKAKYQGTVVLQAVITPD
                     GRATEIQVVKGPGLGLEEKAVEAVKQWRFKPAMGPGGKAVPVTVPIEVTFRLL"
     gene            4431..7307
                     /gene="ileS"
                     /locus_tag="VG272_04345"
     CDS             4431..7307
                     /gene="ileS"
                     /locus_tag="VG272_04345"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HWF12943.1"
                     /translation="MPKIVDLKSTLNLPRTAFPMKAGLPQNEPKQLAEWEEKKLYHRI
                     QQDRADAPTYVLHDGPPYPTGTIHLGTGLNKILKDMVVKSKTMAGFRAPYVPGWDCHG
                     LPIETQVEKELGGKKGSVPPGEFRRMCREFALRYVDQHRRDFKRLGVLGQWEDPYLTM
                     SPHYEATIADAFITFLERGYVYRGLKPVYWCIFDKTALAEAEVEYEDHTSPSIYVRYK
                     LVQNADLKVPQELKGKNVYGVIWTTTPWTIPASMALAFNPEHQYAAVEVEGGDVYIVA
                     QQLQHAVAAACKYVETGNVFGLYTGREIVEKFGLIFQHPFLSDRLEPAVLADYVELDS
                     GTGIVHTAPGHGVEDYQTGQKYGIKAYAPIDDDGRFVEGLPEYKGKAVFEANPIIVQL
                     LESRGALIGQEKISHSYPHCWRCHNPVIFRATEQWFIDLDGAGTGAGPIRSRALAEIS
                     KVKWTPEWGAERIHSMIAERPDWCVSRQRFWGVPLVILYCTKCNKQFDDYAALHALVQ
                     KWFTVEGADAWFTHTVEELLPAGTHCSCGASSWRKETDILDVWFDSGSSHLAVLDRLD
                     AAGQELPWPADMYLEGPDQYRGWFHSSLLVGVAVRDGAPYRQVLTHGWTLDAKGQPMS
                     KSLGNVVLPTEVCEKWGADLLRLWVASQDYTADVRMSDNVMTQLSEAYRKLRNTFRFA
                     LGNLADFDPSRDSLPEAEMEELDRWMLSRMAELVKQCRKWYEGFEFHRVFHAMHDFAV
                     VDLSAFYFDVLKDRLYTFAPRNKSRRSAQTAVYRIAKALLSLTTPITVFTAEEIWKHI
                     PHLAGDPESAHMALFPTKETFGSPFQPAKAANWEKLLLVRTEVLRTLEAARNAKTISG
                     ALEAKVVLSATPDLASVLQQYESWLPGLFIVSQVELADATPADAQKSELLPGLTVTVQ
                     RADGTKCERCWNYSTHVGENSGYPTVCERCVKALAEIEQAQAAS"
     gene            7307..7807
                     /gene="lspA"
                     /locus_tag="VG272_04350"
     CDS             7307..7807
                     /gene="lspA"
                     /locus_tag="VG272_04350"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HWF12944.1"
                     /translation="MRGARWLWVSLAVLATDRATKFAIERTSPIFRHSVVSDIIVLVH
                     SQNPGIAFSMFSESKSSWLAPLLIAVSVAVMALLVWMIVKDRVGGAIAQCGLALILGG
                     AAGNALDRVIHGGVTDFLEVRLWTYIWPAFNLADSAITVGAGLVLFELLFGAQHHAAS
                     GEREQH"
     gene            7973..8971
                     /locus_tag="VG272_04355"
     CDS             7973..8971
                     /locus_tag="VG272_04355"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HWF12945.1"
                     /translation="MIDTPTAHQNLTATSTDAGRRLDLFLAEHVPELSRTRIQELARE
                     GKVRVDGHIAKAAHRIAAGEIIEIEVLPRPAMEALPEDLPLDVLYVDDDFVIVNKPAG
                     MVVHAGAGHARGTLVNALLHRLGKLSEAGGALRPGIVHRLDRETSGAMVVARNDKAHE
                     HLSEQFRSRNVRKIYMALVHGKMPRDSGTITLPISRDANRRTRMTARAKTGRHARTDW
                     RAVARLDGYTLVEVALHTGRTHQIRAHFSAIGHPVVGDTLYGAPRNLRVGKRTLPLLE
                     RNFLHAARIGFSHPSSGAWVEVRAPLPQDLRVYFDQLGASLGWRAAEIEAALALYA"
     gene            8976..10028
                     /locus_tag="VG272_04360"
     CDS             8976..10028
                     /locus_tag="VG272_04360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWF12946.1"
                     /translation="MKKRASWILIVGFLAAVAAVSAQQGQPPAPPQSQSAAPAQSDRQ
                     GTIVRNVNLVEVLFSVVTKREKLITDLTKDNFKVFDDGQQQEITDFSQPTDLPLRIGM
                     VLDTSNSIRERLKFEQDAAIDFLFNALRRNKDQAFLMTFDDGPQIIKDFTGDTGDLRD
                     TILKQRAGGGTSLYDAVHAASNHLLYHSPVPSGPNPDVRRVLVVISDGDDNSSNKSRG
                     EAVDMAQRAGVIIYSISTSTEWVSAEEEKDPAKRITRKYEKNEGDQVLEQLAVETGGR
                     VFFPYKVDDLGQSFLDIGDELRHQYALAYSPTGRAADGKYHAIKVQVDRKDLIVRARK
                     GYYSIPIPVSGKTATP"
     gene            complement(10145..11077)
                     /locus_tag="VG272_04365"
     CDS             complement(10145..11077)
                     /locus_tag="VG272_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWF12947.1"
                     /translation="MRFRAILCYLCAASLVGGLAYAAAPGQIRVQVNQVNVFATVRDK
                     HKSIVTGLTKDDFQVYEDGQLQEITDFSAESNLPVTLGMLLDTSGSEYYMLSAEKDAG
                     SRFFARILRKGDLGLVMSFDTDIDLLADFTDDRGVLNRALNRAQINVPGGGSIAQGPL
                     PTSGSGGTNFYDAVYLAAHDKLSGEAGRKAVVVLTDAEDTGSKLKLQDAVEAAQRTDT
                     VVHILLVAEDGGDQTVAKRLTDETGGRMIVVRNEKNLEAAFDQISEELRFQYTLGYSP
                     SNKSHDGGYRKIKVDMKNKDYSALARRGYYAPNY"
     gene            11189..12316
                     /locus_tag="VG272_04370"
     CDS             11189..12316
                     /locus_tag="VG272_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWF12948.1"
                     /translation="MKCTRKMVVTMLAILLTGSTVAITTSPAQDAKPQEQAPSLEPPG
                     AQKKPQPQMPDVPKQDSRGSIRTTVSLVVVPVTVKNSAGELVTDLQQNDFRVFEDGIE
                     QPIAQFSADAFPLSAAVLVDDDLKRSTAEKVQKTLETLAGGFGTSDEVSLWRFDQVPE
                     QISEDFIADNDALLTQLKKIDLSSSFPGIGSYTMTNGPRVNTAQPPGPAKIPADANGH
                     PNTKHINDAIYAAAEQLQDRPHERRRIIFLISDGQNAKNNTHNYEDTLKLLLSADVSV
                     YVVGVGEANLNRGVTFLGNNILAKYAHATGGDIFYGGVSRENLESLYARVSEQARNQY
                     TIAYSGSHSDKSKSYHSIEVRVKRPGLSLLTRDGYYLNTTP"
     gene            complement(12938..15931)
                     /locus_tag="VG272_04375"
     CDS             complement(12938..15931)
                     /locus_tag="VG272_04375"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HWF12949.1"
                     /translation="MADLFENIGAPAPGIQLNPEQRAAVWHGDGPLVVVAGAGTGKTR
                     VITERIRYLLDTQPGLTGKEILGLTFTDKAASEMKHRVIATSRERGETEAERAKAVDL
                     STFHSFCNTLLQEIDPDLKPIDKIDHWILLRRNLPLLQLEHYRRLAEPGQFLGDFVEF
                     FSRCQDELVTPDDYQGYADKQLQEFQRVRNAMPDDERAIRDEEIAKIQEIARAYRASD
                     LLLRERKLLTFGSQIMNAVLHLRADEDVCERLRSCYKYILVDEFQDTNIAQLELLWQL
                     GGKRPNLVVVGDHRQAIYRFRGASFGSFTIFLNRFATGKETTHEDLLRPLTLNYRSAG
                     RILRVAGQVISHNEKPTNIPEYPLAPVREDGEKVRIVTHSTSEAEAQWVAAELERLHR
                     AGAKWRTFAVLYRGHSHRDKLVDVLKARKIPFVIKNLSILSHRLVRDLIAYLRLIDHP
                     LDDVACARVLAMPAWGLEASDLVRLSERAAKSKGASLWDTMQAAKSEPPFSGGGRNLD
                     SLIELITELRKKARYVTASELFDALAEGLEVSTAVAGEDRKYFDRLGQFVREWQLKSE
                     TQRLKEFVEYLDYFEQAGGSINLEQESGDAVQLMTVHAAKGLEFDHVYVLRVVHRGFP
                     AGEKPRVLEFPPELMKEEQPQGNFHIQEERRLFYVAVTRARQKLTLNTVENKRSKPSP
                     FLDDILMDAQLKRRDVEQLAPAAAGVTFTATEVAQPVLFQMNQQRARIGSQIGEWAMS
                     YRPPVSEPLEISPSGIGTLEACPQKYLFVHSWKLRGGPAAALSFGSVMHNTIKYFISE
                     MMKGHTLPCEEVERKFQLEWTSAGFEDEYQEQEYKKDGLLQLRAFYTSTIAAPPDVLA
                     QEKSFDLSMDNNVVLKGRMDQVNRLGPGEEEIVDYKTGKTRNEEKARKDVQLSVYALA
                     AREVFDWNPARLTLHFLQTNQPVSATRDEKQLKKVRAEIQEAAADIRAGVFPAKPGFS
                     CRFCDFESICPAREQGAAASASGEE"
     gene            16128..16349
                     /locus_tag="VG272_04380"
     CDS             16128..16349
                     /locus_tag="VG272_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12950.1"
                     /translation="MASLSLTLASVQTPIERIFEKVMLRKMTEAEKTVLHVNGHVKPG
                     KKQSRRRVGIFHKNGSELAKKIPAKLTSI"
     gene            16429..17610
                     /locus_tag="VG272_04385"
     CDS             16429..17610
                     /locus_tag="VG272_04385"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HWF12951.1"
                     /translation="MDQAVILSAVRTPIGKFQGGLAPLSAPQLGAKAVAEAVRRAGIE
                     PKAVDEIIMGNVVQAGLGQNPARQAGLGGGLDVRVAAMTINKVCGSGLKAVGLAAQGI
                     MLGENEIAVAGGMESMSNCPYLLPGARTGYRYGNAEVVDSMVRDGLWDVYNDFHMGIT
                     AELVAEKFGITRQEQDQFSLESHQRAVHARKSCFIESQIVPVEIPQKKGEPVVIKYDE
                     SPREDASLESLGRLRPAFKKDGTVTAGNAPGTNDGAAALVVTSERNAARLGKTPMARI
                     VAQAVSGVDPKWIMTAPIYAVEKILEKTGWDRNRDVDLYEINEAFAAAAIAVTRELHL
                     DPAKVNVNGGAVAIGHPIGASGARILTTLLYEMQRRNVHRGIAALCLGGGNAFALAVQ
                     R"
     gene            17824..18342
                     /locus_tag="VG272_04390"
     CDS             17824..18342
                     /locus_tag="VG272_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12952.1"
                     /translation="MTNNSLEILWRGSDPAKFTALQAALRDENIPFWQAQAYDPAGGF
                     LSSRPYYLEATPGFEIRVHAADMERANGALSWVESKEKTFGISAGSQANENDRLDATQ
                     SLPHSWNASEATAEVWSGEDESMAEYLASALRENGIPSRIPDEPGHRARLCVRAQDVT
                     RARQIAREINPE"
     gene            18376..19011
                     /locus_tag="VG272_04395"
     CDS             18376..19011
                     /locus_tag="VG272_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12953.1"
                     /translation="MICPNCKCEYIKGVTQCADCGIPLVDTLDAAPETAERPAVVSVW
                     EGGDPGERAAISEALEEAGIPVVDQEAAGHLFFPSMNPKSDIFVSTENLDRAKKVLAD
                     LEAWDKPEEESDGEPDSEELPESDVTDEGEDDDVDADPSEEWHEDDPVAEVWKGSEED
                     MADTLSMCLREVGIDSHKILEAGIWRLVVRPQKEARAKKVVREVVDASPPE"
BASE COUNT         4395 a         5214 c         5433 g         4254 t
ORIGIN      
        1 gctcggaaga tgcgcgcgtg attcacggcg cagccattcc ggtgtatggc aggagctgag
       61 tagggtgggc tgcagcctgc gagggttgag ctttgcgagg actaaacccc acaggctaaa
      121 gcctatgcca caaaaaaact gctcgcgcgt caaggatttt gcttgggctg ttcgactaat
      181 tcgaccagca gcttttgatt gtccgatgcg gtgaccagga aaaaattcac gcgggtgcca
      241 ttcgctccgg cgcgggtggc cggaaaaatt ggcggaatgt tgaaacgcgt gcgcaggatc
      301 tccgtggcgc gatccacatc cgtcacatca atttcgactt gctgaattcc ttctccaaat
      361 ttatcaagga agcgcgcgat ggcgccgttg ccgcctgacg cttttgtcgt aagaatatcc
      421 agacgcacgt ggggcaatat attctttttg aattccagtt cgtattccag gacatcctga
      481 tcggcaggcg cgtcagctaa aggcaccgag ccgttggcag cgcgaatctt ttcaaatgtt
      541 tcaggaagga cagcgattcc gaccgtcagg ctggcaaaaa aaggaactat tggcgtctca
      601 ctcccataat tttcccggac caaaagcgtc tgaaaatctt tgtcctctac ccaataccgt
      661 agagagatga acacgcgtga gatgcccgtc caaaaaagac gttcagccgt tcttgcgcgt
      721 tcgacgggat cgcgatcggc gagatggcca atcaattcag gggtcaccgg aaccggatta
      781 aaagcatagg tgtaatccgt atatgttttt cccggtcggt ctttcatttg cattttcgcc
      841 ttaataaccg agatgagaat cgcgagtgca ataattcctc ccacaacggt aagagacaag
      901 ccagtggaaa tgtgcaccca tggggcggcc agcatttttg ctccgataaa catgacggta
      961 agggcgaggc cttcatccag atactgaaag tattgcagag ttccagccag gagaaaatag
     1021 agcgcgcgca ggccaagaat cgcgcaaacg ttggagctgt aaacgatgaa gggatcgcgc
     1081 gtgacgccga agaccgccgg aatcgaatcc acggccagca caaggtccgt cgtttccaga
     1141 acgaccacca cgagaaaaag aggggtgaac agccacttgc cactttgccg caaaaatagt
     1201 ttctgcccgg cgccttcctt tgacatgggc agacgcttcc gtgcccagcg catcactgga
     1261 tttttctcgg gatgcacgga gtgatcggcg ccaaacagtt tgaatcctga gtacaagaga
     1321 aacgcgccga agatataaag gatccattca aatctctcga tcagcaccgc gccgaaccca
     1381 atcatcacgc cgcgcagaac gattgcgccg agtacgcccc agaacagcaa gcggtgttga
     1441 taacgcgggt cgacgcgaaa atattggaag atcagaatga aaacaaatat attgtccgcg
     1501 ctgagggatt tttctacgag atagccgctg aagaattcaa ggccaggcgt gcggccatgc
     1561 gaaataaaaa tccatgcgcc gaagccgagc gaaacggcaa tccatccagc gctctccagc
     1621 gccccttcac gccacgtgat cgcatgcggg cggcggtgaa acagcgccag atcgagcagg
     1681 aggaaaaacg cgacgccggc gttaaaagcg atccagatgg acaaagtgcc catctaagcc
     1741 acgctttcgt ctggccggat cactgcggag gaaatcctta attgctcgaa ttttgcgccg
     1801 atgtatttga ccggatggcg ttgaagttgg acagctctat gcttgcagtt tcgccctgac
     1861 ggagtgtaac acgcaaatcc caatgctggc gcacgtctcc ggaaatcgcc tccgcgccga
     1921 acattccaat ccaatagttg ccgggggcaa cacccgagaa cgatccgcgt ccatcgagat
     1981 ccgtttccgc gcgggcgaca acatagcgtt cctcggcaat ttgcatgacg tttgcctcta
     2041 ccatcttcgc atgcttattt tcgagggcaa cccatttcgc aattggattc aggtcgacga
     2101 gttccaggtc catccctttt actgaatccg gcgaagccgc aataaatttg cggacgctgg
     2161 cctcgtactg gtctttctgc tcctggaatt tttctggatt ggccttgcga tctttttcct
     2221 taaacttcgg ctcggggaaa cccatgcccc tgtaggccgc gttatgcgtc atgtaggctt
     2281 tgaaatattc tgagatgccc acgatatctt ccggcgtcag gctcttcgtg aattcctcgc
     2341 cggacaattg cacggagtga tgtttcttca tccaagcttt caattcgggc gaaacttcca
     2401 gcccgtcgac gaacttatcg agatcggggg cgggcgcggc ttgtgtggct tccgcgcgga
     2461 tgtcttgcaa gcttttgcgc aagagataaa aagggagttg gcgaaccggt tccggctggc
     2521 cgccggtcgc tgcgactcgt gcttcaaatt caatccgccc agtggactga gccgcgaggc
     2581 gcgcaggaag caggaatgaa agaacggcaa gcaataaaat cactgcgtga atgtacggtt
     2641 ttgttttcat tgatctcatg gcgaagtgta catcgccgta gcaaagttgg aaacgattct
     2701 gggaggaggt gtttccgcaa tgccagcagg agaaaagaat tagaagtgca tcagtttgga
     2761 ttgcagaggc aaaaaactgc ccgcccgaac agaagggcgg acgctacaaa accaggcggc
     2821 cattccgttc cgttcgcgga gaacgttctc ggtgcaccgt gctcttcggg aaggaatggg
     2881 caactggcac ttattaggat ggcgcgaata aaattttagc gacaggaaaa tagatcagag
     2941 caggcggaac gtaacttcga tgggaacggt caccggaaca gcttttccgc cggggcccat
     3001 ggctggcttg aagcgccatt gctttactgc ttccacggct ttctcttcga ggccaagtcc
     3061 cgggcctttc accacttgaa tctccgtggc gcggccgtca ggcgtaatca cagcctgaag
     3121 aacgactgtg ccctggtatt ttgccttgcg cgcttcttcc gaatattttg gatccgggca
     3181 atatacgcaa gagggaaagc cgacgccgcc ggttcccgga tgataagcgc cgccgcccgt
     3241 cccgccgccg gagcccggac cgagaccgcc gccttcaccg cttccaattc ctgtacccgt
     3301 gccactacca ataccgctac cgccgccggg tccaccggag gtgttcatca gcttggccag
     3361 cggatcgcca tagttatcca tgttgggact cgggattttc aggtccggag gcccaagcaa
     3421 cgttgcttcg attttagatt caggcttggt gtgcaccatc ggaggtgcaa tctgattgaa
     3481 gctgaattta ggcgccttac cctttgtcgc aggaagggga ttgcgttcac cgcctccccc
     3541 gccgccccct gctttatctt ttccgggggg cagtttcatg gtgtagtccg aaatatcgat
     3601 gggcgtgacc agcgaattgt tcaccgggtt cgaggttgcc ttcgtaacta ccgtgtacag
     3661 aaaaggagaa aacacaagaa tcgtaagggc cacgtgcaca ccgagggcaa tggcctgcga
     3721 tggtccaaat gccttatcct tcgtccagat ttcctttacc ctaacgggtt tgctggtgac
     3781 ctgcagcgga ggcagtttcg gcggagaaat caggtcgcgc agattctgcc aaagaacttt
     3841 ataggatggt tgccagtcca ccgtcatgcg tgaagaaacc gctttggggg taggtttgaa
     3901 ccattctttg aaattttcgg caacgccccc accgtattct gctacggtga aaacatcact
     3961 tctcacattc ttgttaattt tcaccggacg ttccgtcagg aagtccttca aattggagac
     4021 aaatccacgc gaaagcggct tctgggaccc gcccagacgc ggagcagtgc tcccctcagg
     4081 caaaacaacc ttaggcacag gcgttcctgg agcactccac tttggcgcaa tcgtactcaa
     4141 atttgtttat gcctcgtgag acgcgaaaat gtcccttgct ctacgattct tctccggtgc
     4201 gaatggatgc cccggcgtgc aatggctgac acatcgtcaa atccattgta acattgtgac
     4261 gttaacacga gaaagcgggt ttccaaacaa cgcggtacag gcacagcggt acaggaagta
     4321 actggccctg ccaagttcgc cgtaactttg gcggattgcg cgcttctcat tcatcatcca
     4381 attagactta gacgcaagga aaaaggaaat cgcgcacaca aaactaactt atgcccaaaa
     4441 ttgttgattt gaaaagcaca ttgaacctgc cgcgcacggc ctttccgatg aaggctggct
     4501 tgccgcagaa tgaaccgaag cagcttgcgg aatgggaaga aaaaaaactc tatcatcgta
     4561 tccaacagga ccgcgccgac gcgcccactt acgttttgca tgacggtcca ccgtatccga
     4621 cgggcactat ccatctgggc accggcctga ataagatttt gaaagacatg gtcgtgaagt
     4681 caaaaaccat ggctggtttt cgcgcgcctt acgtccctgg atgggattgc catgggctgc
     4741 cgatcgaaac gcaggtggaa aaagagctgg gtggaaaaaa gggaagcgtg ccgcccggcg
     4801 aattccgccg catgtgccgc gaattcgcct tgcgctatgt ggatcagcac cgccgcgatt
     4861 tcaagcgcct gggagtgctg gggcagtggg aagatccgta cctgacgatg agcccccact
     4921 atgaggcgac tatcgccgac gcgtttatca catttctcga aagagggtac gtgtaccgcg
     4981 gattaaaacc cgtgtactgg tgcatttttg acaagaccgc gctggccgaa gcagaagttg
     5041 aatacgaaga tcacacaagc ccttccatct atgtccggta caaactggtt caaaacgccg
     5101 acttgaaggt tccgcaagaa ttaaagggaa agaatgtcta tggggtgatc tggacaacca
     5161 ctccttggac aatccccgca agtatggcac tggccttcaa ccctgagcat caatacgcgg
     5221 cagtcgaagt agaaggtggt gatgtatata tcgtcgccca gcaacttcaa cacgcggttg
     5281 ccgcggcttg caagtacgtc gaaaccggaa atgttttcgg attgtacacg ggccgggaga
     5341 tcgtagaaaa atttggtcta atttttcagc atccatttct tagtgatcgg cttgaacccg
     5401 ccgtgcttgc ggactatgta gagctcgatt cggggactgg cattgtgcac accgcgccag
     5461 ggcacggtgt ggaggactat cagaccgggc agaaatacgg catcaaagcg tacgccccta
     5521 tcgacgatga tggcagattc gtcgaaggac tgccggagta caaaggtaag gccgtttttg
     5581 aagcaaaccc aattatcgtt caactgctgg aatcaagagg cgcgttgatt ggccaggaaa
     5641 aaatctcgca cagctatcca cattgctggc gctgccacaa tccggtgatt ttccgtgcca
     5701 cggagcaatg gttcatcgat ctggatggag cgggaaccgg tgcggggccg atccgttcgc
     5761 gcgcgctggc ggaaatttcg aaagtgaaat ggacgcccga gtggggcgcg gaacgcattc
     5821 actcaatgat tgcggagcgc ccggactggt gcgtctcgcg ccagcgattc tggggcgtgc
     5881 cgctggtgat tttgtattgc acgaaatgca acaagcaatt tgacgattac gccgcgctgc
     5941 acgcgcttgt gcagaaatgg tttaccgtcg aaggcgccga tgcgtggttc acgcatacgg
     6001 tggaagaatt gctgccagcg ggcactcatt gttcctgcgg cgcatcgagt tggcgcaagg
     6061 agaccgacat tctggacgtg tggttcgatt ccggttcctc gcatctggcg gtgctcgatc
     6121 ggctcgatgc cgccggacag gaacttccat ggcctgcgga catgtatctc gaagggccgg
     6181 accagtatcg cggatggttc cacagttcgt tgctggtggg cgttgcagtg cgcgacggcg
     6241 cgccttaccg gcaagtgctc acgcacggct ggacgctgga tgctaaggga cagccgatgt
     6301 ccaagtcgct cggaaacgtg gtgctgccga cagaagtttg cgagaaatgg ggcgcggatt
     6361 tgttgcgcct gtgggttgcg tcgcaggatt acacggcgga cgttcgcatg tccgacaatg
     6421 tgatgacgca gctttccgag gcttaccgca agctgcgtaa tacgtttcgc ttcgcgctcg
     6481 gcaatcttgc ggatttcgat ccgtcgcgcg attccttgcc cgaagctgaa atggaggaac
     6541 tcgatcgctg gatgctttcg cgcatggcgg aacttgtgaa gcagtgccgc aagtggtacg
     6601 agggatttga atttcaccgt gtcttccatg ccatgcacga ttttgcggtc gtggacctca
     6661 gcgcatttta tttcgatgtg ctgaaggatc gcctgtacac gttcgctccg cgaaacaaat
     6721 cgcgccgctc ggcacagaca gccgtttatc gaattgcaaa agctctgctg agcctcacga
     6781 ctccgatcac cgtttttacc gccgaggaaa tctggaaaca tatcccgcac ctcgccggcg
     6841 atccggagag cgcgcacatg gcgctgttcc cgacgaagga aacgttcggt tctccgttcc
     6901 agcccgcgaa ggccgcaaat tgggaaaagc ttttgctcgt gcgcacggaa gtgcttcgca
     6961 cgctcgaagc ggcgcgcaac gcaaaaacaa tttcaggagc actcgaagct aaagtggtat
     7021 tgagcgccac gccggatttg gcttcggtgc tgcagcaata tgagagttgg ttgccgggac
     7081 tttttatcgt gtcgcaggtg gaactggctg acgctacgcc cgccgacgcg caaaaatccg
     7141 agttgcttcc tggattgacc gtgaccgtgc aacgcgcgga cggtacgaag tgcgagcgtt
     7201 gctggaatta ttccacgcac gttggcgaaa actccggtta cccgaccgtg tgcgaacgtt
     7261 gcgtgaaggc ccttgccgaa attgagcagg cgcaggcagc gtcctgatgc gcggggctcg
     7321 ctggttgtgg gtttcgctcg cggtgcttgc cacagatcgc gcaacgaaat ttgcaatcga
     7381 acgcacaagc cctattttcc ggcattccgt ggtctcggac atcattgtgc tggtacatag
     7441 ccagaatccg gggattgcgt tcagcatgtt ttcggaatcg aagtcgtcct ggctggcgcc
     7501 gctgttaatt gcggtttcgg tcgcggtgat ggccttgctg gtttggatga ttgttaaaga
     7561 ccgcgtgggt ggcgcgatcg cgcagtgcgg attggctctg attcttggcg gcgcggctgg
     7621 taatgcgctg gaccgtgtca tacacggcgg tgtgacggat tttcttgagg tgcggctgtg
     7681 gacgtacatt tggcctgcgt ttaatttggc tgattcggcg attactgtgg gagctgggct
     7741 ggtgctattt gaattgcttt ttggtgcgca gcatcacgca gcttccggtg agcgggagca
     7801 gcactgagat ctaaaccccc gcctctgatc cctcgtttca ctcgggacaa gcgaggcggg
     7861 ctacaagtgc tgctgtagcc cgggtcttca gacccgggga ttttccgcgc gaggtccgag
     7921 cacgtcttta caacgcaact tttttgcatg gcacgcactt tgaatacaca ccatgattga
     7981 caccccgact gcccatcaaa atttgaccgc aacgtcgacc gacgcgggac gacggctgga
     8041 tttatttctc gctgaacatg tgccggaact cagccgcacg cgcattcagg aattggcacg
     8101 cgaaggaaaa gttcgcgtgg atggacacat agccaaagct gctcaccgaa ttgccgccgg
     8161 cgaaataatc gagattgaag ttttgccacg acctgcgatg gaagcgttgc cggaagattt
     8221 gcctctcgat gtgctttacg tggacgacga ttttgtgatt gtaaacaagc cggccgggat
     8281 ggtggtgcat gcgggcgcgg gtcacgcgcg aggaacgctg gtgaacgcgc tgctgcaccg
     8341 gttgggaaaa ctttccgagg cgggcggagc actgcggcca gggattgtcc atcgactgga
     8401 tcgcgaaaca tcgggcgcca tggtcgtcgc gcggaacgac aaggcgcacg aacatctctc
     8461 cgaacagttt cgctcgcgca atgtacgcaa aatttatatg gcgctggtgc acggcaaaat
     8521 gccgcgcgat tccggcacga tcacgctgcc catttcgcgc gatgcgaacc gccgcacgcg
     8581 catgacggcg cgcgcgaaga cgggtcgcca cgcgcgcaca gattggcgcg ccgtcgctcg
     8641 actcgacggc tacacgctgg tggaagtcgc gctgcatacc gggcggacgc atcaaattcg
     8701 cgcgcatttt tccgccattg ggcatccggt tgtgggagac acgctctatg gcgcgccgcg
     8761 caacttgcgc gtggggaagc gtactcttcc actgcttgag aggaatttct tgcatgcagc
     8821 gcggatcggc ttctcgcatc cgtcgagcgg cgcgtgggtg gaagttcgtg ctccgctgcc
     8881 gcaggatttg cgggtatact tcgaccagtt aggagcatct ttgggctggc gcgccgcgga
     8941 gattgaagct gcgttagcac tgtacgcgta aagacatgaa aaaacgcgcc tcatggattt
     9001 taattgttgg atttctcgca gctgtggcgg ccgtgagcgc gcagcagggc caacctcccg
     9061 ctcctccgca gtctcaatct gcggctcccg cgcagtcgga tcgccagggc acgattgtgc
     9121 gcaacgtgaa tctcgtggaa gttttattca gtgtcgtgac caagcgcgaa aaactgatca
     9181 ccgatttgac gaaagacaat ttcaaggtat tcgatgacgg ccaacaacag gaaattacgg
     9241 atttcagcca gcccaccgat cttccgctac gcatcggcat ggtgttggac acttcgaaca
     9301 gtatccgcga acgtttaaaa tttgagcagg acgcggcgat tgattttctg tttaacgctt
     9361 tgcgccgaaa taaagatcag gcgttcctca tgacgtttga cgacgggccg caaatcatca
     9421 aggattttac gggcgacacg ggcgatctgc gcgacacgat cctgaagcag cgcgcgggcg
     9481 gcggaacttc gctttatgac gccgtccatg cggccagcaa tcatttgctg tatcattcgc
     9541 cggttccttc cgggccgaat ccggatgtgc ggcgcgtgct tgtcgtgatt tccgatggtg
     9601 acgacaacag cagtaacaaa tcgcgcggcg aagccgtcga tatggcgcag cgcgcaggcg
     9661 tgatcattta ttcgatcagc acgagcacgg aatgggtttc agcggaagaa gaaaaagatc
     9721 ctgccaagcg aattacgcgc aaatacgaaa aaaatgaggg agatcaggtt ctcgagcagc
     9781 ttgcggtgga aaccggcggg cgcgttttct ttccgtacaa agtggatgac ctggggcagt
     9841 catttctgga tattggcgac gagttgcgcc atcagtacgc gttggcctac agccctacgg
     9901 gacgcgccgc ggatggaaag taccacgcca tcaaagtgca agtggatcgc aaggatttaa
     9961 tcgttcgcgc gcgcaaggga tattattcca tccccatacc agtaagcggc aagacggcta
    10021 ctccgtaagg gtgggatagg ctgaattcag cctgtgttct tttgactgct tcaaaatggt
    10081 agtaattgag aataagacgc ctcaagcttt tcttactaat gcgtcccgat catgctgttc
    10141 cagctcaata gttaggcgcg tagtatcccc gccgcgccag cgcggagtaa tctttatttt
    10201 tcatatccac tttgatcttg cggtagccgc catcgtgcga cttgttggac ggactatagc
    10261 cgagcgtgta ttggaaacgc agttcttctg aaatctgatc gaacgccgct tccagattct
    10321 tttcattgcg cacgacaatc attcgcccgc cagtttcatc ggtcaatcgt ttggcgacgg
    10381 tttgatcgcc gccatcctcg gcaaccaaca aaatgtgcac gacggtatcc gtgcgttgag
    10441 cggcttccac ggcatcctgc agcttaagtt tgctgcccgt gtcttccgcg tccgttagca
    10501 cgacaacggc tttccggccc gcttcgcctg acagcttgtc gtgagcagcc agatagaccg
    10561 catcataaaa attcgtgccg cctgaaccac tcgtcggaag cgggccttgc gcgatggagc
    10621 ctcctccggg gacattaatc tgcgcgcgat tgagggcgcg gttcaacaca ccgcggtcgt
    10681 cggtgaagtc agcaagcaag tcaatgtcgg tatcgaagct catgaccaga ccgaggtcgc
    10741 ccttgcgtaa aattctggcg aagaagcgcg atccggcgtc cttttccgcg gagagcatgt
    10801 aatactcgct gccgctggta tcgagcagca tgcccagagt aacaggcagg ttcgattccg
    10861 ccgaaaagtc ggttatttcc tgcagctggc catcctcgta gacctgaaag tcatccttag
    10921 ttaggccagt cacaatcgac ttatgtttgt ctcgcaccgt ggcgaagaca ttgacttgat
    10981 tcacctgcac gcgaatctgt ccgggagcgg cagcgtaggc cagccctccc acgagcgatg
    11041 cggcgcacag atagcaaaga atggctcgga accgcattgt ataatctcct ccggctgcgt
    11101 cgacgagttg gatgccgcac tagaacatta agatgcaatg agcgtgccac ccggtggctg
    11161 ctcacgcgga aattggtgct ggaacctaat gaaatgtaca cgaaaaatgg ttgtcacgat
    11221 gctggcgatt ttgctaacgg ggtccacggt tgccatcacg acctcgcctg cgcaagatgc
    11281 taagccacag gaacaggcgc catcgctaga gccgcccggt gctcaaaaaa aaccgcagcc
    11341 gcagatgccc gacgtgccga aacaggattc gcgaggcagc atccggacta ctgtatccct
    11401 ggtggtcgtg cccgtcacgg taaagaatag tgcaggcgaa cttgtaaccg atttgcagca
    11461 aaacgatttc cgggtttttg aagatggcat cgagcaaccc atcgcgcagt tttccgcgga
    11521 tgcgttcccc ttgtccgccg ctgttttggt ggatgacgat ctgaagcgaa gcacggccga
    11581 aaaagttcag aaaaccctgg aaacattggc gggaggattc ggaacatcgg acgaagtatc
    11641 tttgtggcgt tttgatcagg ttcccgagca aatctccgaa gatttcattg ccgataacga
    11701 tgcgctgctc acgcaactca agaagatcga tctgagcagt tcgtttcccg gaatcggctc
    11761 ctacacgatg acgaacggtc cgcgcgtgaa taccgcacag cctccgggac cggcgaagat
    11821 tccggcggat gccaacgggc atcccaacac gaaacatatc aacgatgcga tctacgccgc
    11881 cgcggaacaa ttgcaggacc gtccccacga gaggcgcaga attattttcc tgatttccga
    11941 cgggcagaat gccaagaaca acacgcacaa ttatgaagac actttaaaac ttttgttgtc
    12001 ggcggatgtt tccgtttacg ttgtcggcgt cggtgaagcg aatctgaacc gcggcgtcac
    12061 gtttctggga aacaatattt tagcgaaata tgcgcatgcc accggcggcg atatttttta
    12121 tggcggcgtg agcagggaga atctggaaag tttgtatgcg cgcgtttccg agcaggcgcg
    12181 caaccagtac accatcgcct actccggatc gcactccgat aaatccaagt cctaccattc
    12241 gatcgaagtc cgcgtgaagc gccccggcct ttcgctgctt acgcgcgacg gctattacct
    12301 gaatacaact ccgtaatttt taaaaattag atttctcagt aatgctgaat aggcgcggcc
    12361 gattttgtgg cgccggcgtc tcgccggcgg tttttcttat ccttcacgcg gcgcaaaact
    12421 gccggcaaga tcccggcgcc acgaagataa cccgctccat cgctggattt tgaatttgcc
    12481 atcattacgc gatcccacaa caaaaaaatc ctttcctgcg catttcattt tcatttcgaa
    12541 gttctggaat taattcttga cttccggcgc gtacactcgt aaaacgaaag acagcgggcc
    12601 tgcggaagat ttggcttgct gctccgaggg gagaaaagtt tacggggcga atgcgcgaac
    12661 gggccagaca gataagtaag aaacgggaaa cgcagtaaac cgagtcaggt ctaaagaaaa
    12721 cgtttccagg cggggcgact gtaacaaaca gtcgccccgt ttttttgttt ttgtggcgtt
    12781 ggcgtctcgt cggtgggttt taattttgcg ttgccaatcg gaaccccccg gcggtatgcc
    12841 ggcgccataa aattccagcg ccacgaatat tctgcatggg gcatgaagaa tttaacggcg
    12901 gggttgactg actgaggggg gatgcaaatt acaggtatta ttcttcgcct gacgcgctgg
    12961 ccgccgcgcc ctgttcccgc gcggggcata tggattcgaa gtcgcagaac ctgcatgaaa
    13021 atccgggctt tgcagggaaa actcccgcgc gaatatcggc cgccgcttcc tgaatttcag
    13081 cgcgtacttt cttcagttgc ttttcatcgc gtgtcgcgct gactggctgg ttcgtttgca
    13141 gaaaatgcag cgtgagccgc gccggattcc agtcaaacac ctcgcgcgcc gccaatgcat
    13201 agacgctcag ctgcacgtcc ttcctggctt tttcctcgtt gcgcgtcttg cctgttttgt
    13261 agtcgacgat ttcttcttcg ccggggccga gccggttcac ctgatccatt cgccccttga
    13321 ggaccacatt attgtccatc gacaaatcaa atgatttttc ctgcgcgagc acatcgggag
    13381 gcgcggcaat cgtgctcgta tagaaagcgc gcaattgcag caaaccatct tttttgtatt
    13441 cctgttcctg atactcgtct tcgaatccgg ccgaagtcca ctccagctga aactttcttt
    13501 ccacttcctc gcaaggcagg gtatgcccct tcatcatctc gctgataaaa tatttaatgg
    13561 tgttatgcat cacgctgcca aagctcaagg ccgcggccgg gccgccacgc aatttccacg
    13621 aatgaacgaa taaatatttt tggggacagg cttcgagcgt gccaatcccc gacggactga
    13681 tttcaagcgg ctccgatacg ggcgggcgat aggacatagc ccattcgccg atttgcgaac
    13741 cgattcgcgc acgttgctgg ttcatctgaa acagaaccgg ctgcgccact tcggtagctg
    13801 taaatgtaac gcccgctgcc gcaggagcga gttgctccac gtcgcgtcgc ttgagctgcg
    13861 catccatcag aatatcgtcc aaaaatggcg aaggctttga gcgtttattt tcgaccgtgt
    13921 tcagcgtgag tttttgccgc gcgcgagtca cggcaacgta aaacaggcgg cgctcttctt
    13981 gaatatgaaa atttccctga ggttgctctt ctttcatgag ttcgggagga aactccagca
    14041 cgcgcggttt ttcacccgcc ggaaatccgc ggtgaactac gcgcaaaacg taaacgtgat
    14101 cgaactccag tcccttcgcg gcgtgcaccg tcatcaactg cacggcgtcc ccggactcct
    14161 gttcaagatt gattgagccg cccgcctgct cgaaataatc caggtattcg acaaattctt
    14221 ttaggcgctg cgtttcgctc ttcagctgcc attcgcgaac aaactggcca aggcggtcaa
    14281 aatatttacg gtcttcgccc gccaccgcgg tgctgacttc gagcccttcg gccagcgcat
    14341 cgaacaattc cgaggccgtc acgtagcggg ctttctttcg caattccgtg atgagctcga
    14401 tcagcgaatc cagattgcgc ccgcccccgg aaaatggcgg ctcgcttttt gccgcctgca
    14461 tcgtgtccca gagggaagcg cctttgcttt tcgcggcccg ttcgctcagg cgtacaaggt
    14521 ccgaagcctc caggccccag gcgggcatgg ccaacacgcg cgcgcaagcc acatcgtcca
    14581 gtggatgatc gatcagccgc agataagcga tcaaatcgcg gaccaggcgg tgcgaaagaa
    14641 tcgagaggtt cttgatgaca aatggaattt ttcgcgcttt cagcacatcg acgagtttgt
    14701 cgcggtgcga gtgcccacgg taaagaaccg cgaacgtacg ccacttggcc cccgcgcgat
    14761 gcagccgttc gagttccgcg gcaacccatt gcgcttccgc ttcgctcgta gagtgcgtga
    14821 cgattcttac tttctcgcca tcctctcgaa cgggcgccaa cgggtactcc ggaatattag
    14881 tgggtttttc gttgtggctg atcacctgcc cagccacgcg caggatgcgt ccggcggaac
    14941 ggtaattcaa agtgagcggc cgcagcagat cttcgtgagt tgtctctttg ccggtggcga
    15001 agcgattcaa aaaaatcgta aagctgccaa acgatgcgcc gcgaaaacgg tatatggcct
    15061 ggcgatgatc gccgaccacg accaggttgg gccgtttgcc accgagctgc cacagcaatt
    15121 cgagctgcgc gatattcgta tcctgaaact catccacgag gatgtatttg taacacgagc
    15181 gaagcctctc gcacacgtct tcatcggcgc gtagatgaag aacggcattc atgatttgcg
    15241 aaccgaaggt gagcagtttg cgttcgcgca gcagaaggtc gcttgcgcga taggcccgcg
    15301 cgatttcctg aatcttggca atttcttcgt cgcggatcgc gcgctcgtcg tcgggcatcg
    15361 cgttgcggac acgctgaaat tcttgcagct gtttatccgc gtagccctgg taatcgtccg
    15421 gcgtcacgag ttcgtcctgg cagcgcgaga aaaactccac gaagtcgccc agaaattgtc
    15481 ccggctcagc cagacgccgg taatgctcca gttgcagcag gggaagattg cggcgaagaa
    15541 gtatccagtg gtcaattttg tcgatcggtt tcaggtctgg gtcgatttcc tgaagcaacg
    15601 tgttacaaaa ggaatggaag gtactcaggt cgacggcctt ggcgcgctcc gcctccgttt
    15661 cgccgcgctc gcgcgatgtt gcaatcacgc ggtgcttcat ttcagaagcg gctttatccg
    15721 taaatgtcag cccaaggatt tccttgcccg taagcccagg ctgcgtatcc agcaaatagc
    15781 ggattcgctc ggtgatcacg cgagttttgc ccgtgcccgc tccggccacg acgacgagcg
    15841 gtccgtcgcc gtgccacaca gcggcgcgct gctccggatt gagctgaatc cccggagccg
    15901 gggcgcctat gttttcaaac aaatctgcca tttggaatcg tccagttcat aagaataaca
    15961 cagcgagccg atgtgattaa caagtcagag gtaacattat agttataata gaataaagtt
    16021 aaatatatat actgcctcca gcggaaccgt gataaggtgc ttcagcctta attcaggcat
    16081 agcattttcc ctggagaaaa ccaatttgta gtaaatggag gccctgcatg gctagcttat
    16141 cccttactct tgcatcagtc caaacgccaa tcgagcgcat ctttgaaaaa gtgatgctgc
    16201 gaaaaatgac cgaagcggaa aaaacagtcc tgcacgtgaa tgggcatgtc aaacctggca
    16261 agaaacaatc ccgccgccgg gtaggtattt ttcacaagaa tggctccgag ttggctaaga
    16321 aaattccggc taaattgacc tccatctagg tccctcctat actgggccat ggcgatgtca
    16381 cccttccggg tggcgggctc catttgtctt ttgtccagga gagcaaccgt ggaccaggct
    16441 gtaatcctta gtgctgttcg tacacctatc gggaaatttc aaggtggatt ggctcccctt
    16501 tccgccccac agctcggcgc aaaagctgtc gcagaagcgg ttcgccgcgc gggcattgaa
    16561 cccaaagcgg tggacgaaat cattatgggc aatgtcgttc aagcgggact ggggcaaaat
    16621 cccgcacggc aggcggggct gggcggggga ctcgacgtgc gtgtggcggc catgaccatt
    16681 aataaggtct gtggctcagg cctgaaagca gtcggcttag ccgcgcaggg cattatgctc
    16741 ggcgaaaacg aaatcgcggt cgcgggcggc atggaatcga tgtccaactg cccgtatcta
    16801 ttgcccggcg cgcgaactgg ctatcgctac ggaaacgcgg aagttgtgga ctccatggtg
    16861 cgcgacggct tatgggatgt gtacaacgat ttccatatgg gaattacggc cgagctcgta
    16921 gctgaaaaat tcggcatcac gcggcaagag caggatcagt tttcactcga aagccaccag
    16981 cgtgccgtgc atgccaggaa atcttgcttc atcgaatcgc agatcgtgcc ggtcgagatt
    17041 ccgcagaaga agggcgagcc cgtagtcatt aagtatgacg agtcgccacg cgaggatgct
    17101 tcgctggaat cgctcgggag gctgcggccc gcattcaaga aggatggcac ggtaactgcc
    17161 ggaaacgctc ccggcacgaa cgatggcgcg gcggccctcg tggtgacgag cgagcgcaat
    17221 gcggctcggc taggcaagac accgatggcg cgaatcgtcg cgcaggccgt cagcggcgtc
    17281 gatccgaaat ggatcatgac ggcgccgata tacgcggttg aaaaaattct cgaaaaaact
    17341 ggatgggacc gcaaccgcga cgtggattta tacgaaataa acgaagcatt tgccgcagca
    17401 gcaattgccg tgacgcgcga gctgcatctc gatccggcaa aagtaaatgt gaatggcggc
    17461 gccgttgcga tcggccatcc gataggcgcc tcgggcgcgc gcattctcac gacgttgctg
    17521 tacgaaatgc agcgccgcaa cgtgcatcgc ggcattgcgg cgttgtgtct gggtggcggc
    17581 aatgcttttg cgctggccgt ccagcgttaa aaacgcgttg tgtccctttt tttgtagccc
    17641 gcctcgcttg tcccgagtaa aaacgaggga tcagaggcgg ggtttttgtg ccacggcagc
    17701 aaaattgcca atgtgaagtt tggccagaaa ataagaaaca cccaatcggt atgattccgg
    17761 gtacgcagga acaaatcccc gcctctgaag aggcgggtta cagcagcggc agcagtggca
    17821 aaaatgacca acaactcgct cgaaatattg tggcgcggtt ccgaccctgc gaaattcacg
    17881 gcactgcaag ccgcgctccg cgacgaaaat attcctttct ggcaggcaca ggcctacgat
    17941 ccggccggcg gttttctctc ctcgcgcccg tattaccttg aagcgactcc cggctttgaa
    18001 attcgagttc acgcggcgga catggaacga gccaatggcg ctttgagttg ggtggaaagc
    18061 aaagaaaaaa ctttcgggat ttcagctggg tcgcaagcaa atgaaaacga ccggctcgat
    18121 gcgacgcaat ccctgcccca cagctggaat gcgagcgaag ccaccgcgga agtctggtcc
    18181 ggggaagatg aatccatggc cgaatatctg gccagcgcgt tgcgcgaaaa cggcatcccc
    18241 agccgcattc cggatgagcc cggccatcgc gcccggctgt gcgttcgcgc ccaggatgta
    18301 actcgcgcac ggcaaattgc gcgcgaaatc aacccggaat agatttccgt tacggaaccg
    18361 cgttaacata cgaaaatgat ttgcccaaat tgcaaatgcg aatacatcaa gggcgtcacg
    18421 caatgcgccg attgcggcat tcctcttgtc gatacacttg atgccgctcc cgagacggca
    18481 gagcgtcccg ccgtcgtctc cgtctgggaa ggcggcgacc ccggagagcg cgcagccatc
    18541 agcgaagcgc ttgaagaggc cgggattccc gtggtcgatc aggaagccgc gggacatctt
    18601 ttttttccat cgatgaaccc gaaatccgac atttttgttt ccactgaaaa tctcgatcgc
    18661 gccaaaaaag ttctggccga tctggaagcc tgggacaagc ctgaagagga atcggacggc
    18721 gaacccgatt ccgaggagct tcctgaatct gatgttacgg acgaaggtga agatgatgat
    18781 gtggacgcgg acccttccga agaatggcat gaagacgatc ccgtcgctga agtttggaag
    18841 ggcagcgagg aagatatggc ggataccttg tccatgtgcc tgcgggaagt cgggattgac
    18901 tcgcataaaa ttttagaagc tggcatctgg cgcctggtcg ttcgcccgca aaaagaagcg
    18961 cgagccaaaa aagtcgtgcg cgaagtagtg gacgcaagtc cacccgaata accgtggcat
    19021 aggcttcagc ctgtggaatt tagtcgttgc aaggcccaaa ctccacaagc tgaagagtct
    19081 gagtgagaat tgcagaatcc ttgcgggtcg tggctttagc cacgacataa gtcctgcaaa
    19141 atgagtcccg ctttacagtt tgcggaaaaa gtagtttatt ctgtcattcc gagcgaagcg
    19201 aggaatctct cttcgattta agtataagaa aataaagaag gagggattcc tcgcttcgct
    19261 cggaatgaca aaatgttgat tttttccgca gcctct
//
LOCUS       DASZMB010000095         7855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_648403, whole genome shotgun sequence.
ACCESSION   DASZMB010000095 DASZMB010000000
VERSION     DASZMB010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7855
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648403"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            389..898
                     /locus_tag="VG272_04400"
     CDS             389..898
                     /locus_tag="VG272_04400"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWF12954.1"
                     /translation="MPYQLNLNGKSASVDVPADMPLLWVIRDVLGMKGTKFGCGAGLC
                     GACTVHLNGHAVRSCQTPVSAAANAQITTIEGLSANGTHAVQLAWQELDVPQCGYCQA
                     GQMMSASALLTHSPSPTDVEIEDAMNGNICRCGTYLRIRPAIHRAAVIAQNQSSMPTQ
                     SAHNLVGKE"
     gene            908..3187
                     /locus_tag="VG272_04405"
     CDS             908..3187
                     /locus_tag="VG272_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWF12955.1"
                     /translation="MATTLKQPTLSNSNSIGRRSFLRVSAIGTGGFLLAFYADPISKV
                     FAEGLPKSLPPAPTSPNAKFVPTAFIRVDADGSVTIMAKNPEIGQGIKTALPMIIADE
                     LDVDWKDVKIQQADLDELKFGPQRAGGSTSTPINWDPLRKVGAAGRQMFVSAAAQTWN
                     VPESELTTSAGRVMHSASNRSAGYGELAAKAATLTPPALNAVKVKASKDYKIIGQPKH
                     GVDNASIVVGKPIYSIDFTLPGMLFAVFQKCPVYGGKVVSANLDEIKGMSGVKYAFVV
                     EGLGTDLDRLLPGVAIVADSWWQANTARKKLQVKWDEGATAKESSQGYANRAAELSQQ
                     PPEYVYRKDGDEAAAMQKAAKVVEAAYSYPFLSHAPLEPQNCAAHYKDGKIELWAPTQ
                     TPEAGRQLVASTFGIPPENITIHLMRAGGGFGRRLSNDYAVEAAWISKEIGGPVKLLW
                     TREDDMAHDFYRPGGFHFLKAGLDSNGKIAAWRNHYVAFGDKGVFGYACNMPQNEFPG
                     TFLPDYSLNVSLIPFGQPTGAMRAPRSNALAFVFQSYIDELAHAAGKDPVAFRLEMLS
                     APRINNPAAQPTLREPDINAERMRGVLELVAEKSDWNSRSQLPKGHAKGVAFYFSHLG
                     YFAEVVELSVNEKKQVKIHKIWVAGDIGSQVINPSNAMNQVQGAVIDALSQVMGYEIT
                     FYNGRAMQSNFHQYPPVRIAQAPPEIEVHFLTTANSPTGLGEPALPAILPAVCNAIFE
                     ATGERVRSVPIAKHGYSWA"
     gene            complement(3259..4824)
                     /gene="nadB"
                     /locus_tag="VG272_04410"
     CDS             complement(3259..4824)
                     /gene="nadB"
                     /locus_tag="VG272_04410"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HWF12956.1"
                     /translation="MNSSIQTDILVIGSGLGGLAAAWEAANAGCQVTLLTRAHETQDS
                     NTFWAQGGIIYRGDDEKPEQLQSDILVAGAGLSSPEAASLLSHEGPRLVKKILIDEIH
                     VPFDPSPENPKELDLTSEAGHSISRIIHFKDRTGYSIERAFSERVQAHPNVKVLANAT
                     AVDLLTASHHSVEPTDVYRPLSCVGAYVLDQTTGKISSLLAKETILATGGLGSVFLHT
                     TNPPGARGDGIAMAYRAGARCINLQYVQFHPTALLAPDRCFLISETVRGEGGNLIDRQ
                     GKEFMQRFHPAGSLAPRDVAARAIYQTMLETGEPCVYLDITHKPAEQLRNRFPGIYAV
                     CKERGIDMTKDPIPVVPAAHYSCGGIATDSWGRTTVNRLRAVGEVACTGLHGANRLAS
                     TSLLECLVWGTRAGQQACERILGGDEFYFPQIADWRYEKEPADPALISQDWLTIQHTM
                     WNYVGLIRSEKRLNRAKRVLQELDLEIARFYEKCEISDSIIGLRNGILTALLILDAAD
                     QARESRGCHYRID"
     gene            complement(4990..5817)
                     /gene="nadC"
                     /locus_tag="VG272_04415"
     CDS             complement(4990..5817)
                     /gene="nadC"
                     /locus_tag="VG272_04415"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HWF12957.1"
                     /translation="MLRSFLAEDIATGDITTETTVPEKLRARGELIAKAPLILAGTDL
                     FAEVFRLLDSTTRVEIILHDGEELVPGKIPVRITALARALLSGERVALNLLQRLSGVA
                     TLTRRFVRAVEGTGAAILDTRKTTPGLRELEKYAVRVGGGKSHRKHLAEAVLIKENHI
                     RMAGGVSAALTGAQAAKGRAAWIEIEVTNLEELREALTHKPHVILLDNMGPSLTREAV
                     EEVKRHPGPHQIRTEASGGITLENVRAFAEAGVNWISVGALTHSAPAVDLSFEIEPV"
     gene            complement(5870..6832)
                     /gene="nadA"
                     /locus_tag="VG272_04420"
     CDS             complement(5870..6832)
                     /gene="nadA"
                     /locus_tag="VG272_04420"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006878.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HWF12958.1"
                     /translation="MIIPAQIENVASLAHLDLEEEIARLKKEMNAVILAHYYQESEIQ
                     DLADFVGDSLQLSQQAATTKADVIVFAGVLFMAETAKILNPAKLVLLPDLKAGCSLAD
                     GCPAPLFKAFRERYPDHTAITYINCSAEVKALSDIICTSSNAEKIIRQIPPEKPILFA
                     PDQHLGRFLIKRTGREMILWPGSCMVHEMFSEKSLVQLKERHPKALVIAHPECPESVL
                     RHADYVGSTTGILNFATHNAAPEFIVATESGILHQMIKACPEKSFIPAPGEGGCSCSE
                     CPHMKLNTMEKLYLCMRDRKPEITLDEDLRQRALKPILRMLEMS"
     gene            7102..7644
                     /locus_tag="VG272_04425"
     CDS             7102..7644
                     /locus_tag="VG272_04425"
                     /inference="COORDINATES: protein motif:HMM:NF016184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur transferase domain-containing protein"
                     /protein_id="HWF12959.1"
                     /translation="MKSQFLLVAILISSLWLAGPPARAQEVTKETVDGITNFHRIETT
                     VACSGAIKATAVPDIKKYGFASIINLRQASEDGANLEAEEAAAKSADIHYYSIPFSST
                     APDTAAVDKFLDAITTKGSDPAFIHCAGGGRAATMWFIKRMVVDHWDVDRAAKEATDL
                     GMTSPTLKKFAIDYAQAHKR"
BASE COUNT         1836 a         2250 c         2088 g         1681 t
ORIGIN      
        1 agaggctgcg gaaaaaatca acattttgtc attccgagcg aagcgaggaa tccctccttc
       61 tttattttct tatacttaaa tcgaagagag attcctcgct tcgctcggaa tgacagaata
      121 aactactttt tccgcaaact gaaaagccgc acgcattttg caggacttat gtcgtgccta
      181 aagccacgac ccacaaagat tctgcagttc tcacacagac tccgaagccc gcgctactaa
      241 aacccactcg tctttcaagc cttgccaccg catcggcgcg aaatgctaga attgatcgtc
      301 agatatattt aataaaaaag tcggggcccc agatccgtca ccagatcgct tcaaagctcg
      361 aggcctcgga atttcgggag cgaacacagt gccataccaa ttgaatttaa atgggaagtc
      421 cgcctctgtc gatgtgccgg cggatatgcc cctgctgtgg gtgatccgtg acgtcctcgg
      481 catgaaggga acaaaattcg gctgcggcgc tggcttgtgc ggcgcctgca cagtgcacct
      541 caatggacat gcggtgcgtt cctgccagac gccggtatcg gctgctgcta acgcgcaaat
      601 cacaacgatt gaaggcctat cggcgaacgg cacccacgcc gtgcagctcg cttggcagga
      661 acttgacgtt ccgcaatgcg gctattgcca ggccgggcag atgatgtcag cgtccgcgct
      721 cttgacgcat tcgccgagcc cgacggacgt ggaaattgaa gatgccatga acggaaatat
      781 ttgccgttgc ggaacctacc tccgcatccg gcccgcgatt catcgcgccg ctgtgattgc
      841 ccagaaccaa tcgagcatgc caacgcaaag tgctcacaat ctggtgggga aggaataagc
      901 aaaaatcatg gcaaccacat tgaagcaacc aacacttagc aattccaact cgataggccg
      961 cagatccttt ctccgcgtca gcgcaatcgg cacgggcgga tttttgctgg cgttttatgc
     1021 cgatcccatt tcaaaagttt ttgctgaggg cctgccaaag tcacttccgc ccgcgcccac
     1081 ctctccgaac gccaaatttg tgcccaccgc atttattcga gtggatgccg acggaagcgt
     1141 caccatcatg gcgaaaaatc ccgagattgg gcagggaatc aagacagcac tgcccatgat
     1201 tatcgcggat gaattggacg tggactggaa ggacgtaaaa attcagcagg ccgatttgga
     1261 tgaattgaaa ttcggcccgc agcgcgccgg cggcagcact tcgactccca taaactggga
     1321 tccactccgg aaagtgggag ctgcgggacg ccagatgttt gtaagcgcag ccgcgcagac
     1381 gtggaacgtg cccgaatccg agctcaccac ttcggctggc cgcgtgatgc acagcgcgtc
     1441 gaatcgctcc gccggctacg gcgaacttgc cgccaaggcg gcaactctta caccgcccgc
     1501 gttgaatgcg gtcaaagtaa aagcctcgaa agattacaaa atcatagggc agccgaagca
     1561 cggcgtggac aatgcttcga ttgtggtagg aaaaccgatt tacagcatcg atttcacgct
     1621 tccgggaatg ctctttgccg tgtttcaaaa gtgcccggtc tacgggggca aagttgtcag
     1681 cgcgaacctc gacgaaatta agggaatgtc cggcgtgaaa tacgccttcg tcgtcgaagg
     1741 cctcggaacg gatctcgatc gtttgctgcc cggcgtggcg atcgtcgctg acagttggtg
     1801 gcaggcgaat actgcgcgca aaaaactgca agtgaagtgg gacgaagggg cgaccgcgaa
     1861 ggaaagcagc cagggttatg caaaccgcgc cgcggaactt tcgcagcagc caccggaata
     1921 cgtgtatcgc aaagatggcg atgaagctgc agccatgcaa aaggccgcaa aagtggtcga
     1981 ggctgcatac tcgtatccat ttctgtccca cgcgccgctc gaaccgcaga attgtgccgc
     2041 gcactacaaa gacgggaaga tcgagctgtg ggctcccaca cagacgccgg aagcgggtcg
     2101 tcagttggtg gcctccacgt ttggaattcc tccggagaac atcacgattc acttgatgcg
     2161 ggccggcggt ggattcggcc gtcgtctttc caacgattac gcagtcgagg ccgcgtggat
     2221 ttccaaagaa atcggcggcc ccgtaaaact tttgtggacg cgcgaagacg acatggcgca
     2281 cgacttttac cggcccggcg gcttccattt cctgaaagcg ggcctcgact ccaacggaaa
     2341 aattgcggcg tggcgaaatc attacgttgc gttcggcgac aagggtgttt tcggctacgc
     2401 ctgcaacatg ccgcaaaacg aatttcccgg aacgtttctc ccggattatt cgctgaacgt
     2461 ttccctgatc cccttcggcc agccgactgg tgcgatgcgc gcgccgcgca gcaacgcact
     2521 ggcatttgtt ttccagtcct atatcgacga gctggcgcat gcggcaggaa aagatcccgt
     2581 cgcgttccgc ctggaaatgt tgtccgcacc gcgcatcaat aatccggccg cacagcccac
     2641 gctgcgcgag cccgacatca acgcggagcg catgcgcggc gtcctcgaac tcgtggccga
     2701 aaaatccgac tggaattcgc ggagccaact gcccaaggga catgccaagg gcgtcgcgtt
     2761 ctatttttca cacttgggat atttcgccga ggttgtggaa ttgagcgtca acgagaaaaa
     2821 acaagtaaaa attcacaaga tttgggtggc aggagacatc ggcagccaag tcatcaatcc
     2881 cagcaacgcc atgaatcagg tgcagggcgc ggtgatcgac gcgctgagcc aggtgatggg
     2941 ctacgaaatt actttttaca atggccgcgc gatgcagagt aattttcatc agtatccacc
     3001 tgtgcgaatc gcgcaggcgc cgcctgaaat tgaagtccat ttcctgacga cagcgaattc
     3061 tccaaccggt ttgggcgagc ctgcgctccc agcgattctg cccgcggttt gcaatgcgat
     3121 ttttgaagcc actggcgagc gcgtgcgttc ggtaccgatc gcgaagcacg gatattcctg
     3181 ggcgtagtga ttcgtgaaca acatatccag gtcctctgcc aggtttcacg aattacaaat
     3241 cacgagttac gaatcatatc agtcaattct gtagtggcaa ccgcggctct cgcgcgcctg
     3301 atcagctgcg tccagaatca ataacgccgt gagaattcca ttgcgcagcc caatgatgga
     3361 atcgctgatt tcgcattttt catagaagcg cgcaatttcc agatccagtt cctgcaatac
     3421 tcgcttcgcg cgattcaacc gcttttcgct gcggattaat ccaacataat tccacatggt
     3481 gtgctggatg gtcagccagt cctgcgaaat gagagcgggg tcggcgggtt ccttctcata
     3541 gcgccagtcc gcaatttgcg ggaaataaaa ttcatcgccg cctagaatcc gttcgcaagc
     3601 ctgctgcccc gcgcgcgttc cccacaccag gcattcgagc agcgaagtgc tcgccagccg
     3661 gttcgcgccg tgcaggcccg tgcatgcaac ttctcccacc gcgcgtaggc ggttaacggt
     3721 cgtgcgtccc caactatccg tcgcaattcc gccgcaactg taatgtgccg cgggaaccac
     3781 ggggattgga tctttcgtca tgtcgattcc gcgctctttg cacacggcgt aaattccagg
     3841 aaagcgatta cgcagttgtt cagcgggctt gtgcgtgatg tccagataca cgcaaggctc
     3901 gcccgtctcg agcatcgttt gataaatcgc gcgtgcagcc acgtcgcgtg gagcaagcga
     3961 tcccgcaggg tgaaacctct gcatgaattc cttgccttgg cgatctataa gattgccgcc
     4021 ctcgccgcgc accgtttctg aaatcaggaa gcatcggtcc ggcgcaagca gcgccgtagg
     4081 atgaaattgc acatactgca gattgatgca gcgcgctcca gcgcgatagg ccatggcgat
     4141 tccgtcgccg cgcgcgcctg gaggattcgt tgtgtgcagg aaaacacttc ccaggccgcc
     4201 cgtcgccaga attgtttctt tggcgagtaa ggaggaaatt ttcccggtgg tttgatcgag
     4261 aacatacgcg ccaacgcaac tcagcggacg ataaacatcc gtgggctcga ccgagtgatg
     4321 cgacgccgtg agcaaatcca ctgccgtggc attcgccagc acttttacat tgggatgcgc
     4381 ctgcacgcgc tctgaaaacg cgcgctcgat cgaatagccg gtccgatcct taaaatgaat
     4441 gatgcgcgaa atcgagtgac cagcctcgga cgtcaggtcg agttccttcg gattttcagg
     4501 cgaaggatcg aacggaacat gtatctcgtc gatcagaatt tttttcacga gacgcgggcc
     4561 ttcatggctg agcagggaag ccgcttccgg cgagctgagt cccgcccctg caacaagaat
     4621 gtccgactga agttgctcag gcttctcgtc gtccccgcga taaatgattc caccctgggc
     4681 ccaaaatgta ttgctgtcct gcgtttcatg cgcgcgcgtg agcagcgtaa cctggcagcc
     4741 cgcattggcc gcttcccacg ccgccgcgag cccgcccagc ccgctgccaa ttaccagaat
     4801 gtccgtctga atcgaagaat tcatagtttg tagtaagcct ttgtagcgag cctctgcatt
     4861 gcccttgtag ctcgcctctc ttgccctgag aaaacgcgaa gggtcagagg cgaggattta
     4921 gatgacgccg taccgaaaac cctgccccgg aaaacgcggg caacaaaacc gcagctgcca
     4981 accactttat cagaccggct caatctcaaa acttaaatcc accgccggag cggaatgcgt
     5041 caaagcgccc acggaaatcc aattcacgcc cgcttccgcg aacgccctca cattctcaag
     5101 agtgattccc ccagatgctt cggtgcgaat ctgatgcggc ccgggatggc gcttcacttc
     5161 ttcgacagct tcccgtgtga gcgacggccc catattgtcg agcaggatga cgtgaggttt
     5221 gtgcgtgagc gcctcgcgca attcctccag atttgtaact tcgatttcaa tccacgccgc
     5281 gcgacccttt gccgcctgtg cgccggtaag agccgccgaa acgcctcctg ccatgcggat
     5341 atgattttct ttgatcagta cggcttccgc gaggtgtttg cgatggctct tcccgccacc
     5401 cacgcgcacc gcatatttct ccaactcgcg aaggccgggc gttgtctttc gcgtgtccag
     5461 aattgctgct cccgtacctt ccacggcacg cacaaatcgg cgcgtgagcg ttgcgacacc
     5521 gctcagccgc tgcaaaagat tcaacgctac gcgctcaccg gacaaaagag cgcgggccaa
     5581 ggcggtgatt cgcacgggga tttttcccgg cacgagttcc tcgccatcat gcaaaataat
     5641 ttccactcgg gttgtcgaat ccaaaaggcg aaaaacttcc gcgaatagat cagtccccgc
     5701 aagaatgagt ggcgctttgg caatcagctc gccgcgggcc cgcagcttct ccggtaccgt
     5761 cgtttcggtc gtaatgtccc cggtcgcaat atcttcagct agaaaggacc gtaacaaatt
     5821 gtgatatgcg gaggcgtcga ggggagaggg catatccgaa ggagagagat taactcattt
     5881 ccagcattcg gagaatgggt ttcaaggcgc gctggcgcag gtcttcatcc agcgtgattt
     5941 ccggcttgcg gtcgcgcatg cacaaataga gtttttccat cgtgttcagc ttcatgtgcg
     6001 ggcattcgct gcacgagcag ccgccttcgc cgggagcggg aataaaagat ttctccgggc
     6061 aagccttgat catctggtgc aggatgccac tttcggtggc cacgataaat tcgggggccg
     6121 cattatgcgt cgcgaaattc aaaattcccg tggtcgaccc cacataatcg gcatggcgca
     6181 agacagattc agggcactcc ggatgcgcaa tgaccagagc cttgggatgc cgttccttca
     6241 attgcaccag gcttttctcg gaaaacattt cgtgcaccat gcagctgccc ggccacaaaa
     6301 tcatttcgcg ccccgttctc tttatcagga agcgcccgag gtgctgatcg ggcgcaaata
     6361 aaatcggttt ttccggagga atctgccgaa taattttctc tgcattgctc gaagtgcaga
     6421 tgatgtcgct cagcgccttc acttccgcgc tgcaattaat gtaggtgatg gcggtgtggt
     6481 cagggtagcg ttcccgaaac gccttaaata gcggcgccgg gcaaccatcg gccagcgagc
     6541 agcctgcctt caggtcgggc aaaagcacaa gctttgcagg gttgagtatc ttggctgtct
     6601 cagccataaa tagcaccccg gcaaacacaa tcacgtcagc cttcgtggtc gcggcctgct
     6661 gcgatagttg caagctatcc ccaacaaaat cagctagatc ctgaatttcc gattcctggt
     6721 agtaatgcgc cagaatgacg gcattcatct ctttcttcag ccgcgcaatc tcttcttcaa
     6781 gatccaggtg cgccagtgaa gccacatttt cgatttgtgc cggaatgatc ataattattt
     6841 aaactgccga aattaattct gcaccttcat tatagccatc gcttccaaat gacacaaacc
     6901 gcagaccttc aaaacgccag tcttccaaac ttgattttca accttacgag aagacaccgg
     6961 ttttgtagcg ccggcgggtt ttgcgcctaa acaatatcga acccgcaggc gggacgccgg
     7021 cgctacaaaa ccagattcag aaaactagct ttcggcggga ttttcatcgc ataatgcact
     7081 tacttccatt ggaggtcttc catgaaatcg caatttctac ttgtggcgat cttaatttcg
     7141 agcctgtggc tcgcaggtcc gccagcccgc gcacaggaag taaccaagga aacggtcgac
     7201 ggcatcacga atttccaccg catcgaaacg acagtggcgt gttctggagc aatcaaggcc
     7261 acggccgtac ccgacattaa gaaatatggt ttcgcgtcca tcatcaatct gcgccaggcc
     7321 agcgaagacg gcgccaacct tgaagcggaa gaagcggctg cgaaatcggc tgacattcac
     7381 tactactcca tcccgttcag cagcaccgcg ccggacacag cagcggtcga taaatttctc
     7441 gatgcaatca ccacaaaagg cagcgaccct gccttcattc actgcgcagg cggcggccgg
     7501 gcagcgacca tgtggtttat caagcgaatg gtagtcgacc attgggatgt ggaccgcgcc
     7561 gcgaaggaag caaccgattt gggcatgacc agccctaccc tcaaaaagtt cgccatcgac
     7621 tacgcacaag cacataaacg gtaataatcg ttggtcgtat tttctaaaat tgaatttcgc
     7681 gcatcccgct taaaaagcta tgctttatag gtcgcagctt cagctgcaaa atcaaagctc
     7741 aaagaaagct ggggctttag agtctgcgtg agaactgcaa aatcggaact tcagcggcta
     7801 aacaggctgc ggaaaaagta gtttattctg tcattccgag cgaagcgagg aatct
//
LOCUS       DASZMB010000096        17794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3398600, whole genome shotgun sequence.
ACCESSION   DASZMB010000096 DASZMB010000000
VERSION     DASZMB010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 17794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17794
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3398600"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VG272_04430"
     CDS             <1..326
                     /locus_tag="VG272_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12960.1"
                     /translation="GGVALLIGAGGLIYFKLQSDRAPAEGAAVGMDYVFLVTLGLTAL
                     TGMLTLILRNTSAMGTILVLHLASIAALFLSAPYGKFVHAVYRTLAILRYEIEQSQPH
                     EHVGH"
     gene            554..2578
                     /locus_tag="VG272_04435"
     CDS             554..2578
                     /locus_tag="VG272_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12961.1"
                     /translation="MRGITRVMLWTLLASICIISRPAASSPQSDSGVKVDKSSIGGVV
                     LNSTGGKPEAGVWVIAETKLSVPYRKIVVTDDQGRFLVPDLPDGAYELWVRGYGLKDS
                     EPVKAARGEKVKLQVANATSPQEAAKIYPASYWTSLIQPPTGSDLPARYKTSEEWLGD
                     QRNGCNHCHELGMVATRRYTTVEDWDAVFSRAKAMHQELNFLGKTTVEKVLADWGARI
                     AAGEVPPAPPRPTGIERNVVLTEWDWGSPISFIHDLVSTDKRNPSLYPYGKVYGGDRT
                     GGGRVWILDPVKNTVEGLNLEPRDKSHGFSLTKDYYHGAEEEQAYTGEDKEWMDSPHN
                     PMMDANGKVWMTEQIRAGGAKFYPAWAKDTIALDAADNNDPAAKDIAFNLLSKNGNNM
                     ELGYYDTKTNKFVTVDTAYNTHHLEFDWQDRIWTDGGGNAMGEMDTKKLDFNNIQGTE
                     VAAQKTWMRIDMDTKKMVPGTGYGVAISPIDGTVWETSPQAIGPANKVYMLDPKTGKF
                     KDYPLPAPGRFPHGIDFSTDGNVWVSLGSGHLGRLDVKTGQWKFWENPGVKFKGTGSE
                     TGTTDFPYFLWVDQFNASGLGKDTVIVTGTASDAMFIFDPKKETFSTFHLPYPLPYYT
                     RGLDGRIDDANAGWKGRGLWMTYSSYMPKFTETHKGSVTHMQIRPNPLAN"
     gene            2772..3215
                     /locus_tag="VG272_04440"
     CDS             2772..3215
                     /locus_tag="VG272_04440"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HWF12962.1"
                     /translation="MLATFLITSRITVLMTALLFSAYNVAAQSGETFRARLSPVPINV
                     SMLSTVAGAGSLTATLNGKKLTIQGTFERLRSPATTVQIHRSPKGIPGPAILDLKVTK
                     AEKGTVTGTVDLSPDQIEDLRNGRLYVQIQSQGAPDGNLWGWLLK"
     gene            3322..5493
                     /locus_tag="VG272_04445"
     CDS             3322..5493
                     /locus_tag="VG272_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWF12963.1"
                     /translation="MMKPGSRVSRFAACAAIIFAASLVGNKFVAAQQTARSGPFTSEQ
                     ASAGRTIYAATCSACHLPDMKGTFEAPPLAGPNFMNMWRNRPTSDLFTRIRTAMPLSN
                     PGSLSDEDAANLVAYILQANGATPGTKVLTPETIVPIGAVTTGVAPAPRPEPQEGPAA
                     APRPGAGGPLGLTVSGEVKNYVPVTDEMLLKPDPADWLIVRGNYQGWNHSALSQINHD
                     NVKDLRLIWEWNMNDSVAANEPTPLVHNGIIYLTNTDNIVQALDARTGDLIWENRLRP
                     PRISSGGTGAMRNMAIYQDKVFVATTDAHVFALDARTGKTVWDAILGDTAKGYGSSSG
                     PIIIHGQVVQGENGCDRYKANEKEQGCFISALDPATGKFLWRFNTLARVGEPGGDTWG
                     DLPNMLRVGAETWITGSFDPELNLTYWGVAQAKPWMQVSRGTKGDALYSSSTLALHPD
                     DGKLAWYFQHVQGETLDMDEVYERVLVDIGDRKTLFTIGKAGILWKLDRKTGEFIGAK
                     ETLFQNIFSVNEKTGKLTYRPDILEQEIGKWTPICPSTEGGHNWQAMSYDPATEHLII
                     PLSQSCMEMAGRKVEFVDGSGGTQGDRLFYEMPGTDGNVGRLAAFDVKTMKEVWNVQQ
                     RPSFLTAVLSTAGGIAFVGDINRVFRAIDVNTGKMLWQTRLGTSVQGFPVTFSVGGKQ
                     YVAVTTGLGGGSPRNVPRTILPDVRYPLSGDALYVFALPDK"
     gene            complement(5566..7422)
                     /locus_tag="VG272_04450"
     CDS             complement(5566..7422)
                     /locus_tag="VG272_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HWF12964.1"
                     /translation="MSKLEWIVLAICLVLLLPATSWGERAFAWIEANFSGFAQNRRLA
                     IVALGAIAILARVALLPWQGVPQPKVHDEFSYLLAGDTFAHGRLANPPHPLWFFFDTF
                     HVIQHPSYASMYPPGQGMVLAIGKVLGQPWIGVLLSVAAMCMAFTWMLQAWVPPQWAL
                     VGGVLVWARFSVFSYWINSYWGGAVAATGAALVLGALARIWDHRRLRDAIIFGIGTGI
                     LAISRPVEGAIFTFPVALALLWWAIRSDVETRAAAVKRILLPIAAIVICAVGFVAFYN
                     SRVTGSPTVFPHFIEQKMITTAVFLWQHDKPPISYENPQFEDFYHNFLPSLYQASWPA
                     AVGQWWYKAQDFWEFFLGPVLSIPFLALPWILKKPRNWLLLVQAALSALGLWIIVYYH
                     AHYAAPLMTTVFVLLLQCMVVLRRLRVFGRPMGIGLTRLVVLFSVLIGPTYFLQTVVA
                     QPNALFSWLHRHPVLPIAIAVLGLFVVRMVSILLGRPATCQWRLLGAVCELLLILCIM
                     LQFCEMQRNLYADAFPFVDDVNEPFRKPVEKQLAAIPGEHLVLVRYSKNHNSGEEYVY
                     NEADIDHAKIVWAREIPGVDLKPLLTYFRNRDVWVYEPDEDDSTVRPYAGAQ"
     gene            7522..9408
                     /locus_tag="VG272_04455"
     CDS             7522..9408
                     /locus_tag="VG272_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12965.1"
                     /translation="MANLISAEQTLPADARKSVLPSPKDSPSSADTGTFWNRSTFALF
                     TLVALWAVKLYTTWGAWGNLTIDSGHELYVPAMLAQGKQLYRDLWFPFGPGAPYFTSY
                     LFRLFGARLNVLYWAGSISALGSAIFLYLTGKRLSYSLAGWTAGAIVLMEAFQPSLFC
                     FPLPYSSAAVYGCFTACVFLWLIVSACYSGERVWLLRSATAAALALILKPEFGLAAYG
                     CVSLLIAFRALSQKSWRNFGRDILASLPGVAVCGLVIGWMVSIAGARFITQENIQSWP
                     TSYFLKTYGKTWLETTGFTVSGSAFVAAFHRAIPVAAMVISLFFLFRWKGTDKKSLLG
                     KAGIVFILLWYFNQMNYFALSLKQSLTLLSSLIFFPRDMVLYVILGAAAAWCYFLLKH
                     FAARNLAVPIVLTFSSLIAFRVLMKMNTLGYPIYYNGPVVLSYLLLLCMLIPRAKRSR
                     QFVILGEAALCLACLAPVFVHTRAIEAGARYFIPLTTDYGTIRVSPHMADGYKTAIQF
                     IKEKADLGKTVLSVPEDTSLYFLSGTVCPTRVFLFIPGAVAPGKMTDETTRQIERKPV
                     DYLLWSNRTFTEYGAKEFGKDFNPELGEYLTSHYRPIYPLVTSDKPESDWTAVVWERK
                     PDVK"
     gene            9533..9928
                     /gene="rplS"
                     /locus_tag="VG272_04460"
     CDS             9533..9928
                     /gene="rplS"
                     /locus_tag="VG272_04460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HWF12966.1"
                     /translation="MNLVIEKFQSSQVRKDLPSLRPGDTVRVHVRLKEGEGDKERIQP
                     FEGVIIKKRGKSTGGSFTVRRVSFGVGIERIFPANSPSISSIEVLRQGRVRRAKLYYL
                     RGLKGKAARLQRVERVETPETPATEETKS"
     gene            10085..10750
                     /locus_tag="VG272_04465"
     CDS             10085..10750
                     /locus_tag="VG272_04465"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HWF12967.1"
                     /translation="MTRDCSTRFEREARKCGWQRIAGLDEVGRGSLFGQVVAAAVILD
                     PKRRIVGLDDSKKLPAERREVLAMRIREHALAWAIAEVDASRIDAWNIYQASRMAMAE
                     ALKLLKISPDYLLLDAMQLDVLIEQKSLIHGDALSVSIAAASIIAKVERDRQMREWDQ
                     IYPQYGLAQHKGYATPEHLAALRAHGPTPHHRYSFYPVREAACWAAGATQVALPLPSG
                     EIA"
     gene            11034..13652
                     /locus_tag="VG272_04470"
     CDS             11034..13652
                     /locus_tag="VG272_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF12968.1"
                     /translation="MAYNKTKHLNAAQKYLQQGKMPQAILEYQQILKNEPKDQVTLMT
                     LGDLFVRQGDTFQALEYFERLAKIFLNDGFTTKAIAIYKKVAKLAPEESRPLERLAEL
                     YVQQGVLSEARPLYLQLAEMQLKAGRQPQAALLLRKLLEAEPDNLRVQVRLAELHLSM
                     GQQKEAIETFYGAAQKLHEQGDHVEAIRLADRILELDSLHKPTFQLKVHALNAAGKSA
                     EAGKILETLPDRDEGGETTSMLLNLYMESGQIVQASQLAEKVYARDPKQFAVPLKITG
                     LLLDTGNLDRAQDMLDLVRNSMIDAGEHEPLAQLLTRLAENKPGKIEPIEWLVDLYGR
                     ASDSFRLPDALAQLAAAYESAGDEAKALATYEKLLERTPEDETTRRKYMRLRSKSGLE
                     PISSEIASVVKVVPTNDAAQPVSIKSSDPALDEETERYVTQALTDVDLFSSYGLAQKA
                     IDLLESVLDRAPRHAPILERLLDLSVGSGNSGRIAELASILEQIAKEQNDRAGVERYA
                     ELRRRFQPGAGATSEGQESSAQPAQAAAPEFAVPVIRAELDESAEQAQPEAEFSVPAQ
                     IPEPVEVESSVHEVDLSDEWAALSQQLETAMMDEPKPAFQQSALPQQKMEIPAPVLVP
                     AQAVQSDAEATSQIPGYDFELQAAEPVGVTQSDTHAADSMISDLAGDLDSYASSLGIS
                     NVPPLPAPKSSIPAPVEPTTKSSAKPTSGNGATAPVSSGSLEGPLGDLFEEFRSELGE
                     KKEDEDLETHYNLGIAYREMGLLEEAISEFQKVASSLDKGPAFRYAMQCSTLLGLAFM
                     EKGQPAIAAIWYERALKTPGLDQESILALRYDLGVAQELAGDEKAAFNSFSQVYALNI
                     DYRDVSERIALLGKAR"
     gene            13664..13906
                     /locus_tag="VG272_04475"
     CDS             13664..13906
                     /locus_tag="VG272_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12969.1"
                     /translation="MKSFLHVLLTLDVFLLGVLLIMVPWYGYWDHNFFLDKFPSLIPI
                     LLHPSMRGAVTGLGTLDVLLASSMLRGRTDSVANRT"
     gene            13890..14573
                     /locus_tag="VG272_04480"
     CDS             13890..14573
                     /locus_tag="VG272_04480"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HWF12970.1"
                     /translation="MRIERKPIFCYVTDRRSFELVPASIRPERGRVLLPEVNSLHDSI
                     SRVIFAGVNWIQIREKDLDTRTLLEIARFAVAKAHATSTRVLINDRLDVALAAHAAGV
                     HLGEKSLPLESVAAWRRTSRRTDFQIGVSCHSLESACAAESGGADYVFFGPVFATPSK
                     AAFGAPQGIDRLREVCASVKIPVLAIGGVNPENTRDCLKAGASGIAAIRLFQNAKDSD
                     ELTASLKRI"
     gene            complement(14671..15345)
                     /locus_tag="VG272_04485"
     CDS             complement(14671..15345)
                     /locus_tag="VG272_04485"
                     /inference="COORDINATES: protein motif:HMM:NF006731.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HWF12971.1"
                     /translation="MKPRIVRNIERPDPELARKLGEFGTATVHEAQGRTGLMLPYMRP
                     IYPAARIGNPAVTVLCHPGDNLMFHAAIEVCKPGDVLVVATHSDSTDGFFGELLATSC
                     QAHGIRGLVIHAGVRDVADLTAMQFPAFSRAISAQGTVKETAGYVNIDIVCAGALVHP
                     GDIIVGDVDGIVVVSRQDAGEVVRACEARLAKEAKNRERLKAGELGLDMYGLREKLKN
                     LGVEWV"
     gene            complement(15410..16354)
                     /locus_tag="VG272_04490"
     CDS             complement(15410..16354)
                     /locus_tag="VG272_04490"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HWF12972.1"
                     /translation="MTAVSPSAGEGGAKVTPVTNSAWKCLEGAYDMQVHVAPDVIERR
                     VDDLDLAKEFLGRGLKGFVLKSHYFPTAERAKVVTKAVPGIAAYGAITLNHSVGGLNP
                     VALELAGRSGCKIVWFPTVDANNETAGRLDGPNVKLPFWAKIQRELAATGIAPPPLTV
                     LDLEGRTSEITKRCVEIIAKHNMILATGHLGRMEIFELVKTAREMGTQRVLVTHAEFP
                     SQNLTADEQKHLADMGAYIEHCFTTMHTGKAPWEAVMEAIRVVGPERCVLSTDLGQTI
                     NPPVAEGFAMFTQKMLDAGFSVDQIRRMTVTNPSALLA"
     gene            complement(16597..17304)
                     /locus_tag="VG272_04495"
     CDS             complement(16597..17304)
                     /locus_tag="VG272_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12973.1"
                     /translation="MKNRIGILYAFLFATIFAFAGILFAQSKPNRALIVNGKQQGDVV
                     QMNGHAYIDVETVAQLMNGSVTLEPNRVVLSFPEPQPAEAQAAAPEPQQPSQPQVPPG
                     ALTRNFASLAIGELAEMREWRGAVGTILTYGVPVVGTWPQDYHDRVQANLDQVSVSAS
                     TPGDEDAMRLLQNEFSNLSRWTNDVVSARQSLNATNSVRPDALQSDANLAKISNCSRF
                     LSSMLVSGTFADDQSCH"
     gene            complement(17497..>17794)
                     /locus_tag="VG272_04500"
     CDS             complement(17497..>17794)
                     /locus_tag="VG272_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWF12974.1"
                     /translation="TPVVHIVMNNSSLAFEYHVQKYVCEEMCPEASEFLDVPFGDVAR
                     AFGAHGERVTSADQFIPALRRAEESGKPAIVDVVVSRELPPPVTRYEAAGLRKI"
BASE COUNT         4160 a         4987 c         4734 g         3913 t
ORIGIN      
        1 taggcggcgt agcgctcctc attggagcgg gtggattgat ctacttcaag ttgcaaagtg
       61 atcgcgcgcc tgccgaagga gccgcagtcg gcatggatta cgttttcctc gtcaccctcg
      121 gactcacggc tctcacgggg atgctgacgc tcatcctgcg gaacacttcc gccatgggaa
      181 ccattctggt gcttcatctc gcgtccattg cggcgctgtt tttatccgcg ccttatggaa
      241 aatttgtgca cgccgtgtac agaacgctcg ccatccttcg ctacgaaatc gagcagagcc
      301 agccgcacga acacgtaggg cactagataa aaattcacat cctcaagtgg agtgggcttc
      361 agcctgcggg tttttgttct tgcaaagcct aacccccgca ggctgaagcc cgcgctacat
      421 aacccttcaa taagtaatat ccgaaaccaa atcaatggcg atttcgtcca cttcattata
      481 taaagcccag tacagctggt gtgagctgta tccaagccta taagtcaagg cgaattgcaa
      541 ggagaagacc gatatgcgag gaatcacgcg cgtgatgttg tggacgcttc tagcatcaat
      601 ttgtattatt tcgcggcccg ctgcgagcag tcctcagagc gactccggag tgaaagtcga
      661 taagagcagc attggcggcg tcgtgctgaa cagcacaggc ggaaagcccg aggctggtgt
      721 ctgggtgatc gccgagacga aattgtcggt gccgtatcgc aagattgtgg tcacggacga
      781 tcaggggcga ttcctggttc ctgacctgcc cgatggagcc tacgagctat gggtgcgcgg
      841 ctatggactt aaggattcag agccggtaaa ggcggcgcgc ggcgaaaaag tgaagctgca
      901 ggttgccaac gcaactagtc cgcaagaagc ggcgaagatc taccccgcaa gttactggac
      961 gtcgctgatt cagccgccga cgggaagcga tctccccgcc aggtacaaga cctcggaaga
     1021 atggcttggg gaccagagga acggttgcaa tcactgccat gaactcggca tggtcgccac
     1081 gcgccgctat acgactgtgg aagattggga cgctgtcttc tctcgcgcta aggcgatgca
     1141 tcaagaactc aattttctcg gcaaaactac cgtagagaag gtcctcgccg attggggcgc
     1201 ccgcatcgcg gccggagaag ttcctcccgc tccgccgcgg ccaaccggca tcgaacgcaa
     1261 tgtcgtttta actgagtggg attggggatc cccaatttcc tttattcatg atttagtctc
     1321 tacggacaag cgcaatccat cgttgtaccc atatgggaaa gtctacggtg gggatcgaac
     1381 cggcggaggc agggtatgga ttttggaccc ggtgaagaac accgtcgaag ggcttaacct
     1441 cgaaccaaga gacaaaagcc atggtttctc cctgacaaag gactactacc acggggcgga
     1501 agaagagcaa gcgtatacgg gcgaagacaa ggaatggatg gacagtccgc ataatccaat
     1561 gatggatgcg aacggcaaag tctggatgac cgagcagatt cgcgccggcg gcgccaaatt
     1621 ctatccagcg tgggccaagg atacgatcgc cctggacgcc gcggataaca atgatccggc
     1681 tgcgaaggat atcgcgttca atcttctgtc caaaaacggt aacaatatgg agctcggtta
     1741 ctacgacaca aaaaccaata aatttgtcac cgtcgacacc gcctacaaca cccatcacct
     1801 cgagttcgat tggcaagacc ggatctggac ggacggcggc ggcaatgcga tgggtgaaat
     1861 ggacaccaaa aagctggatt ttaacaatat tcagggaact gaggtagctg ctcagaagac
     1921 ctggatgcgc atcgatatgg acaccaagaa gatggttcca ggaacgggct atggcgtggc
     1981 tatcagtcct atcgatggca ccgtttggga aacatctccg caagccattg gtccggcgaa
     2041 caaggtgtac atgctcgatc cgaagacggg caaattcaaa gattacccgc tacctgctcc
     2101 gggacgtttc ccgcatggca ttgacttcag cacggacggg aacgtttggg tttcgctcgg
     2161 cagcggtcat ctcggccgcc tagacgtcaa gaccggccag tggaaattct gggaaaaccc
     2221 gggagtaaaa tttaagggca ccggcagcga aaccggcacc accgatttcc cctacttcct
     2281 gtgggtagat caattcaacg cgtcgggtct tggcaaggac accgtgatcg tcactggaac
     2341 cgcttcggac gcaatgttca tattcgatcc gaagaaggag acattctcta cattccacct
     2401 tccttacccg ctgccctatt acacgcgtgg tttggatggt cgaattgacg atgccaacgc
     2461 gggttggaag ggccgaggcc tttggatgac ctacagctcc tacatgccca aattcacgga
     2521 aacgcacaaa ggctcagtaa cccatatgca aattcgcccc aatccattgg cgaattaaca
     2581 cggaaattcg ttggcaaatt taagcccacg tcttggccaa atctgcgcgt taaatttagg
     2641 aagggcattt gcccttccgc gcagcatttg gccgagctgt gcgaacgcta tctgaatttc
     2701 aaatccaaaa atgaaattca ctgtttccta agattggggc catgatagtg aaacgacgtg
     2761 aaagacatat agtgttagca acgtttctga tcacctctcg aatcactgtt ttgatgactg
     2821 cgcttttatt ctccgcgtac aatgttgccg cgcaaagtgg tgaaacattt cgggcgcgac
     2881 tctcgcctgt cccgatcaat gtttccatgc tatccaccgt tgcaggcgcc ggatcgctta
     2941 cggccacgct gaacggcaaa aagctcacaa ttcagggaac attcgaaagg ctgcgctcgc
     3001 ctgcaaccac cgtgcagatt catcgcagtc caaagggaat tcccggcccc gcgattctgg
     3061 atttaaaggt tacaaaagcg gaaaagggga cggtcaccgg cacggtggat ctttcgccgg
     3121 accagattga agatctgcga aatggccgct tgtatgtgca aatccaatcg cagggtgcgc
     3181 ccgacggaaa tttgtggggc tggcttttga aataatcggc gtagcgccca ctttcaggtc
     3241 ggcacgtgcg ggagagcgca aaatgcccga cctgaaggtg ggcactacaa tgattggttc
     3301 agaaatatta ctgagaaaat tatgatgaaa ccaggatctc gtgtaagccg cttcgctgca
     3361 tgtgctgcaa ttatttttgc cgcatcgctt gtcggaaata aatttgtcgc ggcgcaacaa
     3421 acggcgcgca gtggcccttt cacatccgag caggcaagcg cagggcgcac catttatgca
     3481 gcgacctgct cagcctgcca cttgcccgac atgaaaggca cgttcgaagc gcctccgctc
     3541 gctggcccca attttatgaa catgtggcgc aaccgcccga cgagcgacct cttcactcgc
     3601 attcgaaccg cgatgccgct gagcaacccc ggctcgctca gcgatgagga cgccgccaat
     3661 ctggtcgcct acatattgca ggccaatggc gcgacgccgg gtacaaaggt gctgacaccg
     3721 gagactatcg taccgattgg agctgtcaca acgggcgtcg cgcctgcccc ccgacccgag
     3781 ccacaagaag gcccggcggc agccccgcgg cctggcgcag gaggacccct gggcctcacc
     3841 gtttccggcg aagtgaaaaa ttacgtgccc gttacggacg aaatgcttct caagccagac
     3901 cccgccgact ggctcatcgt ccgcgggaac tatcagggat ggaatcacag cgcgctctcg
     3961 caaatcaatc acgacaacgt gaaggatctc cgcctcatct gggaatggaa catgaacgac
     4021 agcgtggcgg ccaacgagcc cacgccgctg gttcataacg gaatcatcta tctgaccaac
     4081 acagacaata tcgtgcaggc gctcgacgcg cgcaccggtg acctcatctg ggaaaaccgc
     4141 ctgcgacctc cacgaatctc atccggcgga accggcgcca tgcgcaatat ggccatctac
     4201 caggataaag tttttgtcgc tacgaccgat gcgcacgttt ttgcgctcga cgcgcgcacc
     4261 ggaaaaacgg tctgggacgc gatccttggc gacacggcaa aaggctacgg aagttcgagc
     4321 ggcccaatca tcattcacgg ccaggtcgta caaggcgaga acggatgcga ccgttataag
     4381 gcgaatgaaa aagagcaggg ctgcttcatt agcgcgcttg atcccgcgac cggaaaattt
     4441 ttgtggcgct tcaacacgct ggcgcgagtg ggcgagccgg gcggtgacac gtggggcgat
     4501 cttcccaaca tgctgcgtgt cggcgcggaa acctggatca ccggcagctt cgatcccgaa
     4561 ctgaatctga cgtattgggg cgttgcgcag gccaagccgt ggatgcaggt cagccgcggc
     4621 acaaaaggcg acgcgctgta ttccagctca acgctcgcgc tgcatcctga cgatggaaaa
     4681 ctcgcctggt acttccagca tgtgcagggc gaaacgctgg acatggacga agtgtacgag
     4741 cgcgtgctgg tcgatatcgg cgaccgcaaa acacttttca cgatcggcaa ggccggaatt
     4801 ctctggaagc tcgatcgcaa gaccggagaa tttattggcg cgaaggaaac attgtttcaa
     4861 aatattttca gtgtgaatga aaaaacagga aagctcacct accggccaga catccttgag
     4921 caggaaatcg gcaagtggac tcccatttgc ccaagcacgg agggcggcca caactggcag
     4981 gccatgagct acgatccggc caccgagcac ctcatcatcc cgctcagcca gagctgcatg
     5041 gaaatggcag ggcgcaaggt ggaatttgtc gacggatctg gtggcacgca gggcgaccgc
     5101 ctcttttacg aaatgcccgg cacggatggg aatgttgggc gcctcgcagc gttcgacgtg
     5161 aagacgatga aagaagtttg gaatgtgcag cagcggccgt cgttcctcac ggctgtgctt
     5221 tcaaccgctg gcggcatcgc ctttgttggc gacattaacc gcgtattccg cgccatcgat
     5281 gtaaatacgg gcaaaatgct ttggcaaacg cgcctcggca cgtctgtgca gggattccct
     5341 gttaccttca gcgtaggcgg caaacaatac gtggctgtga ccaccggcct cggcggtggc
     5401 agtccgcgca acgtcccgcg tacgatcttg cccgatgtgc gctatccgtt gagcggcgat
     5461 gcgctttacg ttttcgcgtt gccggataaa taggtttcgt ttttcagtga cgtgggcttt
     5521 agtctttgca gggccaaaac ccagcaggct tcagcctatg ccactttact gcgccccggc
     5581 atagggccgc acagtagaat catcttcgtc cggctcatac acccaaacat cacggtttcg
     5641 aaaataagtc agcagcggtt tcagatccac gccaggaatt tcacgcgccc atacaatttt
     5701 tgcgtgatcg atgtccgcct cattgtagac atattcttcg ccggaattgt gatttttcga
     5761 ataccgcacc agcacaagat gctcgcccgg aatcgccgcc agttgctttt ccacaggctt
     5821 tcgaaacggt tcattcacgt catccacaaa aggaaaagca tccgcatata aatttctttg
     5881 catttcacaa aattgcagca taatgcaaag gattagcaga agttcgcaga ctgcgccaag
     5941 caatctccat tggcatgtcg cggggcgccc taacagtatg gagaccattc tcactacaaa
     6001 taatccaagc accgcgatcg caatcggtaa caccgggtgc cgatgtagcc agctgaacaa
     6061 ggcgttcggc tgcgccacca ccgtctgcag aaaataagtc ggcccgatca gcacactgaa
     6121 aagaacaacg agccgcgtca atcctattcc cataggccta ccaaacacgc gcagccgccg
     6181 gagtacgacc atgcattgca gcaacaatac aaataccgtg gtcatgagcg gcgcggcata
     6241 atgcgcgtgg taatacacaa tgatccataa tcccaacgca gaaagcgctg cctgaacgag
     6301 aagcagccag ttgcgaggct ttttcaaaat ccatggcagg gcaagaaacg gaatagaaag
     6361 cacgggcccc agaaaaaatt cccaaaaatc ttgcgccttg taccaccact gtccgacggc
     6421 ggcaggccaa ctcgcttgat agaggctcgg caaaaaattg tgatagaaat cttcaaattg
     6481 cggattctca tacgaaatgg gcggcttgtc atgttgccaa agaaaaactg ccgtggtgat
     6541 cattttttgt tcgatgaaat gcgggaacac ggtgggactg ccagtcacgc gtgaattgta
     6601 gaaagcaaca aatcccactg cgcaaatcac aatcgccgcg ataggcagga gaatgcgctt
     6661 cacggcggca gcgcgtgtct ccacatccga tcgaatggcc caccacaaga gtgcaagggc
     6721 cactggaaac gtaaaaatcg cgccttccac aggacggctg atcgcaagaa tgcctgttcc
     6781 aattccaaaa ataatcgcat ctcgcaagcg ccggtgatcc cagattcgcg cgagtgcacc
     6841 caagacgagc gccgctccgg ttgccgccac cgcgccgccc caataactat tgatccagta
     6901 gctaaagact gaaaagcgcg cccaaaccag gacgccaccc actagcgccc attgcggcgg
     6961 gacccacgct tgcaacatcc acgtgaatgc catgcacatg gccgccacac tcagcagaac
     7021 gccgatccat ggttgcccca atactttgcc gattgccagt accatccctt gccctggcgg
     7081 gtacatcgag gcgtacgacg gatgttgaat cacgtgaaat gtgtcgaaaa aaaaccaaag
     7141 cggatgcgga ggattggcaa gccggccgtg agcgaatgta tccccagcca gcagataact
     7201 gaactcgtca tgcactttgg gctgcggcac accctgccat ggcaacaggg ccactcgggc
     7261 gaggatcgca atcgcgccca gcgcgactat cgcgagccgc ctgttttgcg cgaaaccaga
     7321 aaaattcgcc tctatccagg caaaggcccg ctctccccaa ctcgtagcgg gaagcagcag
     7381 caccaggcaa attgcgagaa caatccactc taatttagac acgcgaatcc gtccggcgcg
     7441 agccgcagta ggttacctct taacgactcc tcgcggcgtt tcaaacctac gccgcgcaat
     7501 ccccttgcta tcataaaaat catggccaat ttgatttcgg cggaacaaac gctccctgca
     7561 gatgcgcgga aatccgtttt accctcaccg aaagattccc cctcctcggc ggatacagga
     7621 acattttgga atcgcagcac atttgcctta ttcacgttgg ttgccctgtg ggctgtgaaa
     7681 ctgtacacga cttggggagc ctggggaaac ctaaccatcg attccggcca tgaattgtac
     7741 gtgcccgcga tgcttgctca gggaaagcaa ttgtatcgcg acctgtggtt tccgtttggg
     7801 cccggcgcgc cctacttcac cagttattta tttcgtctgt ttggcgcgcg tctaaatgtt
     7861 ttgtactggg cagggtcaat ctcggcgctt ggctccgcga tatttcttta ccttaccggg
     7921 aaacgcttat cctactcact ggcgggctgg actgcaggcg ccatagtcct gatggaggca
     7981 tttcagccct ccctgttttg ttttccattg ccgtattctt ccgcggccgt gtatggatgt
     8041 ttcacggcat gcgtatttct ctggctgatt gtcagcgcgt gctattcagg cgagcgagtt
     8101 tggctgctcc gctcagcaac cgcggctgct ctcgcgctaa tactaaaacc ggaatttggg
     8161 cttgcagctt atggctgcgt gtccttattg atcgcgttcc gcgctctatc tcaaaaatcc
     8221 tggagaaatt tcggcaggga tattttagcc tcgctgcccg gtgttgcggt ctgcggcctc
     8281 gtcattggct ggatggtttc gattgcaggc gctcggttca tcacgcagga aaacattcaa
     8341 agctggccta catcgtactt cctgaaaaca tatggaaaaa catggctgga aaccactggc
     8401 ttcacggttt ctgggtccgc gttcgttgct gcgtttcacc gtgccattcc cgtggccgcg
     8461 atggtcattt ctctattttt tctttttagg tggaagggga ccgacaaaaa atcgttgttg
     8521 ggaaaggccg gcatcgtctt catcttgctc tggtatttca atcagatgaa ttattttgcc
     8581 ttgtccttaa aacaatctct gactcttttg tcgtcattga ttttctttcc gcgagacatg
     8641 gttctgtacg ttattctcgg cgccgctgct gcctggtgct atttcttatt aaaacatttt
     8701 gccgcgcgga atttggctgt gccaattgtt cttacttttt caagtttaat cgcgttccgc
     8761 gtgttgatga agatgaatac gttgggctac ccgatttatt acaacggccc ggtcgtcctt
     8821 tcctatcttc tcctcctttg catgctgatc ccgcgagcca agcggtcgcg ccagtttgtg
     8881 attctagggg aggcagcgct ctgcctggct tgtttggccc cggtatttgt ccacacaaga
     8941 gcaatcgaag caggagcccg gtatttcatt ccacttacaa cagactacgg caccatccga
     9001 gtttccccgc acatggctga cggttacaag accgccattc aattcatcaa agagaaagcg
     9061 gatctgggaa agactgttct ctccgttccc gaagacacga gcctttactt tctttccggc
     9121 accgtctgcc ccacacgcgt ctttttgttt attcccggtg ccgtcgcccc cggaaaaatg
     9181 accgacgaaa ccactcggca aatcgagcgc aagccggttg attacctgct ctggtcaaac
     9241 cgcacgttta cggagtacgg cgcaaaggag tttgggaagg attttaatcc tgaactgggc
     9301 gagtatctga catcccatta ccgccctatc tatccgctgg ttacgagtga taaaccggaa
     9361 tcggattgga ccgcagtggt ttgggaacgc aaaccagatg taaaataggg acttgcgccg
     9421 accgtatccg tcctcagttt gttatggtgc aatcaggcta tttccgctat actttgcgtt
     9481 tgcattttgc agtgctgacc ccgcctgagt gggtctgccg ggaatcgaag ccatgaatct
     9541 ggtcattgaa aagtttcaat cgtcacaagt gcgtaaagac ctgccttcgt tgcgtccggg
     9601 tgatacggtg cgcgtgcacg tccgcctaaa agaaggcgaa ggcgataaag agcgtattca
     9661 accgtttgag ggcgtaatca tcaagaagcg cgggaagtcg acgggcggaa gcttcactgt
     9721 tcggcgcgtg agtttcgggg tgggcattga gcgtatcttc ccagcgaact caccctccat
     9781 cagttccatc gaagtgctgc gccagggccg tgtacgccgc gccaagctgt actacctgcg
     9841 cgggctgaag ggcaaggcgg ctcgcttgca gcgcgtagag cgcgtggaaa caccggaaac
     9901 cccggccacc gaagaaacaa agtcctaaga cttcctgcga accttagctt ccaacaaagc
     9961 ccagggcaat ccgccctggg ctttttcatt tacattcatt gcgctttaca agcacgcgtt
    10021 tccttatcta actttttcat tctgaatgct catcttaatt tccgctgtgt taaaatccgc
    10081 cgtgatgacc agggattgtt ccaccaggtt cgagcgtgaa gctcgcaagt gcggctggca
    10141 aagaattgcc ggtctcgatg aagtcggccg cggatctctt ttcggtcagg tcgtcgccgc
    10201 agccgtaatt ctcgatccca agcgtcgcat tgttggcctg gacgactcca aaaaattgcc
    10261 ggccgagcgg cgtgaagttc tggcgatgcg aattcgcgag catgcattag cctgggcgat
    10321 tgcggaagtg gacgcgagcc gtatcgacgc gtggaatatt tatcaggcca gccgcatggc
    10381 gatggcggaa gccttaaaac ttttgaaaat ctctcccgat tatttgcttc tggacgccat
    10441 gcagcttgac gtcttgatcg agcagaaatc cctcattcac ggcgacgcgc tttccgtttc
    10501 cattgcagcc gcgtcaatca tcgcgaaagt ggaacgcgac cggcagatgc gcgaatggga
    10561 ccaaatttat ccccagtacg gactcgcaca gcacaaaggc tacgccacac ccgaacattt
    10621 agccgctttg cgcgcgcacg gccccacgcc gcaccatcgg tattcttttt atcctgtgcg
    10681 cgaggcggca tgctgggcgg cgggcgcaac ccaagtggca ttgccgctgc cttcaggtga
    10741 aattgcctga gacaccaaga aaccgaacaa tgggtctcgc gggtctccgc aacccccctc
    10801 attactgcca cgtacgcttt taaatgcctc gcgttgactt cggattttgc cgcgtgctag
    10861 cgtgaaatct aagcttgtca gggacatagt tagatggtcc cgcaggctgc gctattttct
    10921 tcgtcgcggt cttttgcctc gcttgaggcc aaactggagt tgcggcgaat caacgatcca
    10981 ccattgccct ttttcattcg ttttgacagg ggcaatggaa agagcatctc aagatggcct
    11041 ataacaagac aaagcactta aatgcggcgc aaaaatatct ccaacagggg aagatgccgc
    11101 aggccatcct cgaataccaa caaattctta agaatgagcc aaaagaccag gtcacgctga
    11161 tgactctggg agacttattt gtccgccagg gtgatacgtt tcaggccctc gaatattttg
    11221 aacggctggc aaagattttc ttgaatgatg gattcacgac caaagccatc gccatttata
    11281 aaaaagtagc caagctagct cctgaagaaa gccgtcctct cgagcgcctg gctgaacttt
    11341 acgtgcagca gggagtcttg agcgaggcgc gtcctcttta tttgcagctt gctgaaatgc
    11401 aattgaaagc ggggcgccaa ccgcaggctg ccttgctgct gcgaaagttg ctcgaagcgg
    11461 aacctgataa tttgcgcgtg caggtaaggc tcgcggaatt gcatctttcg atggggcaac
    11521 aaaaagaagc catcgaaaca ttttatggag cagcgcaaaa gctgcacgag caaggcgatc
    11581 acgtggaagc catccgcctg gcggatcgca tcctggaact ggattctttg cacaagccaa
    11641 ctttccaatt aaaagttcat gcattaaacg ccgccggaaa aagcgcggag gccgggaaaa
    11701 ttctggaaac gcttccggac cgcgatgagg gcggcgaaac aacatccatg ttgctgaatc
    11761 tgtacatgga aagtggccag attgttcagg cgtcccagct cgctgaaaaa gtttatgcgc
    11821 gtgatcccaa acaatttgca gtgccgctca aaattaccgg actgctgttg gacacgggaa
    11881 atctggaccg cgcgcaggac atgctcgatt tagtccgcaa ttcaatgatc gatgcaggtg
    11941 agcatgaacc tctggctcaa ttgctcacgc gccttgcgga aaacaaaccc ggaaagattg
    12001 agccgatcga gtggctcgtc gatctttacg ggcgggccag cgactcgttc cgcttgcctg
    12061 atgcgctggc tcagctcgcg gccgcctatg agtccgcagg cgatgaagca aaagcgctcg
    12121 cgacctacga aaagctgctg gagcgaactc ccgaagacga aaccacacgc cgcaaataca
    12181 tgcgtttgcg ctcgaagtcg ggattggaac cgatctccag tgaaattgcc tccgtcgtga
    12241 aggtggtccc gacgaacgat gccgctcagc ccgtcagtat caaatcgtct gatccggcgc
    12301 tggatgaaga aacagaacgc tatgttacgc aagctttgac tgacgtcgat ttattttcca
    12361 gttatggcct tgcgcagaaa gcgatcgacc tgctggaatc ggtcctggac cgcgcgccgc
    12421 gccatgctcc tatcctcgaa agattgctgg atctctccgt tggctcgggt aacagcggac
    12481 gcattgcgga actggcaagc attctggagc aaattgcgaa agagcaaaac gaccgtgcgg
    12541 gcgtggaaag atatgccgaa ctccgtcgtc gtttccaacc cggcgcaggc gcaacttctg
    12601 agggccaaga atcgtcagct caacccgcac aagctgctgc accggaattt gccgtccctg
    12661 tgatccgagc ggagcttgac gaaagcgcgg aacaggctca gccagaagcg gaatttagtg
    12721 tccccgcgca aattcctgag ccggtcgaag tggaatccag cgtgcacgaa gtggatcttt
    12781 ccgatgagtg ggctgcgttg tctcagcaac ttgagacggc gatgatggac gaaccaaagc
    12841 ctgccttcca gcaatccgcg ctcccgcagc agaaaatgga gattccagct ccggtcctgg
    12901 ttccggcaca ggccgtccaa tccgatgcag aagcaacctc gcaaatcccg ggatacgatt
    12961 tcgaactgca ggcagctgaa cccgtaggag tgacgcaaag cgatacccac gcggcggatt
    13021 cgatgatctc cgatcttgcg ggagatttag attcttatgc ctcgtcattg ggaatttcga
    13081 atgtgccacc gctccctgcg ccaaaaagtt cgatccccgc gcccgtcgag cctacaacca
    13141 agagttctgc caagcctaca tccggcaacg gagccacggc accagtatcc tcgggatcac
    13201 tggaaggacc cttgggcgac ctcttcgagg aatttcgcag cgagcttggc gaaaaaaaag
    13261 aggacgaaga tctcgaaact cactataacc tgggaatcgc ttaccgtgaa atgggtctgc
    13321 tggaagaagc catcagcgaa ttccagaaag tggccagcag cctggataag gggcccgctt
    13381 tccgttatgc catgcaatgt agtacccttc tgggattggc atttatggag aaggggcaac
    13441 cagccattgc cgcaatctgg tacgagcgtg ctcttaaaac acctgggctt gaccaggaat
    13501 cgattctcgc gttgcgctac gatttgggag tcgcccagga gttggcggga gacgagaagg
    13561 cggcattcaa cagcttctct caagtgtacg cgctgaacat cgattaccgc gacgtatcgg
    13621 aacggattgc ccttcttggg aaggcgcgct aaccgcacct cgaatgaaat cattcctgca
    13681 cgtgttgctc accctggacg tttttctgct cggcgtgttg ctgatcatgg ttccctggta
    13741 cggctattgg gatcataatt tttttctcga caagtttccc agcctgattc ctattcttct
    13801 ccacccttcc atgcgcgggg ctgtaactgg tttgggaacc ctcgacgttc tgcttgcctc
    13861 cagcatgcta cggggacgga cggactccgt tgcgaatcga acgtaaacca attttctgtt
    13921 atgtgacgga ccggcgatcg tttgaacttg tgccagcttc cattcgccct gagcgcggcc
    13981 gcgtcctcct gcctgaagtc aattcgcttc acgattccat ttcccgggtg atttttgcgg
    14041 gagttaactg gattcaaatt cgcgagaaag accttgacac gcgtaccctg ctggaaattg
    14101 cgcgctttgc cgttgccaaa gcgcatgcga cctccacgcg agtgctgatc aacgatcggc
    14161 tggacgtggc gctggccgcg catgccgcgg gggttcacct cggtgaaaag tccttacccc
    14221 tcgaatccgt agcggcgtgg cggcgcacca gcagacgaac ggattttcag attggcgttt
    14281 cctgccattc ccttgaatcc gcgtgcgcgg ctgaatccgg cggcgccgat tatgtttttt
    14341 tcggcccggt atttgccact ccatccaagg cggcattcgg cgcgccgcaa gggatcgatc
    14401 ggctccgcga ggtgtgcgct tccgtaaaaa ttcctgtgct ggcgattggc ggcgtgaatc
    14461 cggaaaatac gcgagattgt ctaaaagcgg gagcctctgg aatcgccgcg atccgattat
    14521 ttcagaatgc gaaggattcc gatgaactta cagccagctt aaaaagaatt tagccttaag
    14581 tagaacaggc ttcagcctgt tttcttttga cgctttcaag ggcagtcatg cggtgatgga
    14641 caaaaaccaa acagactgaa gcgtatacta ctaaacccat tcaactccca gatttttcag
    14701 cttctcgcga agtccataca tatccagccc caattcccca gccttcaggc gctcgcgatt
    14761 tttcgcttct ttggccaggc gagcttcgca cgcgcgcaca acttcacccg catcctgccg
    14821 cgagaccacg acaatgccgt caacgtcccc gacgatgata tcgcccggat gaaccagcgc
    14881 gcccgcgcag acgatgtcga tgttcacgta gccggcggtt tcctttacgg ttccttgcgc
    14941 ggaaatggcg cgcgaaaatg cggggaactg catggcggtt aaatcagcga cgtcccgtac
    15001 gccggcgtga atgaccaacc ccctgattcc gtgagcctgg caggaagtcg cgagcaattc
    15061 tccgaaaaat ccgtcggtgg aatcagaatg ggttgccacg acaagaacgt cgcccggctt
    15121 acacacttca atcgcggcat ggaacatcaa gttgtcgccc ggatgacata gcacagtcac
    15181 agccggattc ccgatgcgcg cggccggata aatcggccgc atatacggaa gcatcaggcc
    15241 ggtccggccc tgcgcttcgt ggaccgttgc tgtgccgaac tctccgagtt ttcttgccag
    15301 ctcaggatca ggccgttcaa tgttgcggac gattcttggc ttcatggaaa attctccgtc
    15361 tattgaattt cgtcagcgaa catgcttact caatttttct cgttcgtttt tatgcgagga
    15421 gcgcggacgg attcgtgacc gtcattcgcc gaatttgatc gacgctgaat ccggcatcca
    15481 gcattttctg cgtaaacatc gcaaaacctt cggcgaccgg cggattaatt gtttgcccca
    15541 ggtccgtgga taggacgcag cgctccgggc ccaccacgcg aatggcctcc atcacagctt
    15601 cccacggagc ttttcccgtg tgcatggtgg taaagcaatg ctcgatgtac gcgcccatgt
    15661 ccgcgagatg tttttgctcg tcggcggtta aattttgcga tggaaattcg gcgtgagtca
    15721 ccagaactct ttgcgtcccc atttctcgcg cggtcttcac caactcaaaa atttccatgc
    15781 ggcccagatg ccccgttgcc agaatcatat tgtgcttggc gatgatttcg acgcagcgtt
    15841 tcgtgatttc cgaagtcctg ccttccaggt cgagcaccgt cagcggcgga ggcgcaatcc
    15901 ctgttgccgc aagttcgcgt tgaattttcg cccagaacgg cagcttcaca ttgggcccat
    15961 ccaggcgccc ggcagtttcg ttattcgcat cgacggtagg aaaccacacg atcttgcagc
    16021 cggagcgtcc ggccaactcc agcgccacgg gattcagccc gcccacggaa tgattcagcg
    16081 tgatcgcgcc gtaagcggca atgcccggca cggctttcgt gacgaccttc gcgcgctcgg
    16141 ctgtcggaaa gtaatgcgat ttcagaacaa agcctttcaa tccgcgaccc agaaattcct
    16201 tcgccaaatc cagatcgtca acgcgccgct caatcacgtc aggcgcgacg tgaacctgca
    16261 tatcataagc gccttccaga cacttccacg cggagttcgt aaccggcgta acttttgctc
    16321 cgccttctcc ggcgcttggc gacactgcgg tcatatcggg tagtcctttt cgttcgatga
    16381 tttatttccc ggctcaagca aattgcccgc tccaacaaaa aaaacggcgc gcaaatttaa
    16441 gcaaatacct aaaaactaaa aaccatttaa tgaagttgca ggcacagaat atacctcatc
    16501 ttcaagacat ttccctatag ggggtaagca gcgcgggctt cagcctgcgc tgctcacccc
    16561 cactgaaact tatttcccta ctaccgcacg gaaaaactaa tggcagcttt ggtcatcggc
    16621 aaatgtcccg ctgacgagca tggaactgag gaaccggctg caattggaaa tcttggccag
    16681 atttgcatca ctttgcaggg catcggggcg aactgaattt gtagcgttca acgattggcg
    16741 cgcggaaacc acatcattcg tccatcgtga caaatttgaa aattcgtttt gaagtaggcg
    16801 catggcatct tcatccccgg gcgttgaagc cgagacactc acttgatcaa ggttcgcttg
    16861 cacgcgatcg tggtaatcct gcggccacgt tccgacgacc ggcactccgt aggtaagaat
    16921 cgtgccaacc gctccgcgcc actccctcat ctccgccaac tcgccaatcg ccaggctcgc
    16981 aaaatttctg gtgagcgctc cgggaggaac ttgtggctgc gacggttgct ggggttccgg
    17041 cgcagcagct tgagcttcgg ccggttgagg ctccggaaaa gaaagtacaa ctcgattggg
    17101 ttccagcgtc acggagccat tcatcagttg cgccacggtc tccacatcga tatacgcgtg
    17161 gccattcatt tgtacgacat ctccctgctg ctttccattc acgatcagtg cgcgatttgg
    17221 tttgctttga gcgaataaaa ttcccgcaaa cgcaaatatc gttgcgaaca gaaaagcgta
    17281 gaggattccg atgcgatttt tcatgatgac cccgcttcca tggaaatcgg gctgcggacg
    17341 accgccaata cataggttac agcgattgca gtggagctac tagcggcagt tcccgaaaga
    17401 tttgatgcca ctaagcgtaa gaaagaagat gcgcaaaata aaaaagaggc gaactgcgcg
    17461 tcaatcgcgg cagttcgccc cttgtaattt taccgcttag atcttgcgca gtccggccgc
    17521 ttcgtagcgc gtcacgggcg gcggaagttc gcggctcacc accacgtcga cgatcgcggg
    17581 cttgccggat tcctcggcgc ggcgcaatgc gggtatgaat tgatcggcgg aagtgacccg
    17641 ttcaccgtga gctccgaatg cgcgggccac atcgccaaac ggcacatcca gaaattcgct
    17701 cgcctcgggg cacatttcct cgcacacata tttctgcaca tgatattcga atgcgagcga
    17761 gctgttgttc atcacgatat gcacgacagg agtc
//
LOCUS       DASZMB010000097         5258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2569906, whole genome shotgun sequence.
ACCESSION   DASZMB010000097 DASZMB010000000
VERSION     DASZMB010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2569906"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VG272_04505"
     CDS             <1..989
                     /locus_tag="VG272_04505"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: protein motif:HMM:NF016438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HWF12975.1"
                     /translation="NGHILLTKYPNCAKALDKYRINPGNVGAGARRDEQFSTICKVAV
                     DNGKPVRIGVNGGSLNQELVMRRMQENTDRDLGLSSEDIINTCMVQSAIESTELALEC
                     GLRHDQIIISCKLSNPQNLIKVYRELSSKTQQPLHLGLTEAGMGTKGIVWSSAALGIL
                     LQEGIGDTIRISLTPRPGGDRREEVYAACELLQSLGLRSFAPSVTACPGCGRTTSTTF
                     QELAERIQDYIRESMPQWKLQYEGVEEMTLAVMGCIVNGPGESKAANIGISLPGTGEE
                     PSCPVYIDGKKSVTLHGNYDELSVAFRKLVDDYIQSRYAPKSPAPASASSGR"
     gene            1056..1583
                     /locus_tag="VG272_04510"
     CDS             1056..1583
                     /locus_tag="VG272_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12976.1"
                     /translation="MKKLFFSIAAVVAIFAASSSRAAGKPQPAKADQNQPTKVWTNED
                     LDELRARGLISFVGQEPTAPAAQPAAAAPQSTHPVYESRLDDPTWYGDQATALRAELD
                     RREGALNEELSAIALAADRVTQPGVALEKPSEGVTPEAAVQVLQEKVQEVQNQLDELS
                     DLARQHGIAPGVLRG"
     gene            complement(1636..3699)
                     /locus_tag="VG272_04515"
     CDS             complement(1636..3699)
                     /locus_tag="VG272_04515"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HWF12977.1"
                     /translation="MLRSKTRICTVLALALSLTSATASAQTVAVTTKNSALAGSPNDL
                     SQLALEDLMNIKVTSVSKTDQKMSQVAAAIFVITDEDIQRSGATNIPDLLRMVPGMDV
                     SQINANAWAVSARGFDGQFSDKLLVLIDGRAVYTVLLGGVNWDTQNVPLEDIGRIEVI
                     RGPGATVWGANAVNGVINIVTKTAADTKGFLASGGGGTEGKAFGLVQYGGSLPKNTDY
                     RVAINYLNSGSLPAEDGGSDHDNWNLLHGSFRADTKISPTDSLTVQGDMYTGQAGDTI
                     IHIYSINPPVVGDQNVNDQLAGGNVLGRWNHVFSSRSDTTLQFYYDSFDRSGPESDES
                     RRTLDFDFNHHFQWGSRQDVVWGIGYRRTWDTDFGTIDQSFNPHDTTLQLFTVFAQDT
                     FTLHPNCLFLTAGTKLENSYFTGLDLDPSARLAWTPSSTMTLWAAVSRAARSPDRRDT
                     ELDAALTAFPDPNGSSTPVEVVLFGNPNFQNEHILAYEAGFRTQPNARLSVDVSTFFN
                     RYDHLETLESGARFLQSAPAPARFVVPITFGNLMYGSTEGGELSTNFKLTDRWTLSPG
                     YAFLEMHLHVKPTSGDATSVAEYQGSSPQHQVQLRSHVELPLGLLWDSSAYFVSALTA
                     QGVPSYTRLDTQLTWRFAERGEISLVGQNLLRDTHLESMDQLTVVNSSLMKRSAYAKL
                     TYLFW"
     gene            complement(3940..4695)
                     /locus_tag="VG272_04520"
     CDS             complement(3940..4695)
                     /locus_tag="VG272_04520"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWF12978.1"
                     /translation="MWNEPEQIFIAPDGQAENPKRAAVGPASFSASFKPAFRLQESFT
                     APLERKVLPWLAGKLPARMNSDHLTLLGFVAMFLAGASYAFARTNRLGLLLATFFLAL
                     NWFGDSLDGTLARLRNRQRPCYGFYVDHMVDTFGGLFLMGGLALSGLIDWRIALAMFI
                     AFLMLSVQVYLATYTVGTFQLSFAKFGPTEIRLLLSAGNMALWFHPDARIFGLSYRLF
                     DAGGVIAAAGMSGMLVIATICNTAKLYRAETLS"
     gene            complement(4728..>5258)
                     /locus_tag="VG272_04525"
     CDS             complement(4728..>5258)
                     /locus_tag="VG272_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12979.1"
                     /translation="TVFISGVSLRQVLALVQDYDHHYRYYSPEVVKSKILEHKSNDFK
                     VYLRLKKKKVVTVVLDTEYQVHYHTLDATRAFSESYSTRIAEISHPDEPGERASPEGQ
                     GEGFMWRLNSYWRFAERDGGVYVQCEAISLTRDVPAGLNWLIAPFIDSIPRESLEFTL
                     ASTRTAVLRGGTHALQ"
BASE COUNT         1263 a         1463 c         1420 g         1112 t
ORIGIN      
        1 acaacgggca catactcctg acgaaatacc cgaattgcgc caaggccctc gataaatacc
       61 gcatcaatcc aggcaacgtt ggcgcgggcg cgcggcgcga tgagcagttt tccaccatct
      121 gcaaagtagc ggtcgacaac ggcaagccgg tacgcattgg cgtcaacggc ggctcgctga
      181 accaggaact cgtaatgcgg cgcatgcagg agaacaccga ccgggacctc gggctcagtt
      241 ccgaagacat tattaacacc tgcatggtcc agtccgcgat tgaatcgacc gagctggcac
      301 tggaatgcgg cttacggcat gaccagatta ttatttcctg caagctgtcc aatccgcaga
      361 atttgatcaa ggtgtatcgc gagctttcga gcaaaactca gcaacccctt catctggggc
      421 tgactgaagc cggcatgggc accaaaggaa ttgtgtggtc gtccgcggcg ctgggtattt
      481 tgctgcagga aggcatcggc gacacgattc gcatttcgct cacgccgcgt cccggcggcg
      541 accgtcgcga ggaagtgtac gcggcttgcg aactgctgca atcgctgggc ctgcgctcct
      601 tcgcgccgag cgtcacggct tgccctggtt gcggccgcac gacgagcact acgtttcagg
      661 aactcgccga gcgcattcag gattacattc gcgagagcat gccgcagtgg aagctgcagt
      721 atgaaggcgt ggaagaaatg accctcgccg tgatgggctg catcgtcaac ggccccggtg
      781 aatcgaaagc cgcgaacatc ggcatcagcc ttcccggcac gggcgaagag cctagctgcc
      841 ctgtctacat cgacggaaaa aaatccgtga ccttgcacgg caattacgac gaactttccg
      901 tcgccttccg taaactggtc gacgactaca ttcagtcccg ctacgcgccg aaatctcccg
      961 cgcccgcctc tgcctcttcc ggcagataat cattttcaat tctctgcaaa cgtaaaaaag
     1021 cggtacactc gagttgtccc cgccgaggag gattcatgaa gaaattattt ttttcaatcg
     1081 cggcggtagt tgcaatcttc gcagcaagtt cttcgcgagc ggcagggaaa cctcaacccg
     1141 ccaaggccga ccaaaaccag cccacgaaag tttggacgaa tgaagatttg gatgaactgc
     1201 gcgcgcgcgg attgatttct ttcgttggcc aggaaccgac cgcaccggcc gcgcaacctg
     1261 cagccgcagc cccgcaatcg acgcatcccg tttacgaatc caggctggac gatcccacct
     1321 ggtacgggga tcaggcaacc gctttgcgcg ccgaactcga ccggcgcgaa ggtgctttga
     1381 acgaggaact atccgcgatc gcgctggcgg ccgaccgggt cacgcaaccg ggggttgcgc
     1441 tggaaaaacc cagcgaaggc gtaacgcccg aagcggcagt tcaggtgctg caggagaaag
     1501 tgcaggaggt gcagaatcag ctggatgaat tgagcgatct tgcgcggcag catggtattg
     1561 cgccgggcgt gctgcgcggt tgaattgttg gagcagatag ctcaatttga aaaaatccac
     1621 aggctgaagc ctgtgctacc agaagaggta tgttaatttt gcgtatgcgc tgcgcttcat
     1681 cagcgacgaa ttcacaacgg tcagttgatc catggattcg agatgcgtat ctctcaaaag
     1741 gttttggccg accaggctga tttcccctcg ttccgcaaat cgccatgtta gttgcgtgtc
     1801 gagacgcgtg taggacggaa cgccttgcgc agtaagcgcg ctgacgaagt acgcactaga
     1861 atcccataac agcccgagcg gcagttctac atgcgagcgg agctgcacct ggtgttgagg
     1921 acttgaaccc tggtattccg cgacggatgt tgcgtcgcca ctcgtgggtt tcacgtgcag
     1981 atgcatttcc aaaaatgcat accccgggct gagcgtccag cgatcggtca atttgaaatt
     2041 cgtggacagc tcaccgcctt ccgtactccc atacatcaag tttccaaacg taattggcac
     2101 cacaaaccgc gccggcgccg gagccgattg aaggaacctg gcgcctgact cgagggtctc
     2161 caagtgatcg tatcgattga aaaacgtaga gacgtcgacg gagagccgcg cgttcggttg
     2221 cgttcggaaa ccggcttcat acgccaatat atgttcgttc tgaaaattgg ggttcccaaa
     2281 tagaacaact tcgaccggcg tgctcgatcc gttgggatcg ggaaatgccg tgagcgccgc
     2341 atccagctcc gtgtcccttc gatccgggct acgcgcggcc ctggaaacgg cggcccaaag
     2401 cgtcatcgta ctggaaggag tccaagccaa tcgcgcgcta ggatccagat cgagaccggt
     2461 gaagtaacta ttttccagct tggtccccgc ggtcaaaaac aggcaattgg gatgcagtgt
     2521 gaatgtgtct tgcgcgaaga cggtgaagag ttgaagtgtg gtgtcgtgcg gattgaacga
     2581 ttggtcaatt gtcccaaaat cagtatccca ggttcgacga tagccaattc cccacacgac
     2641 gtcttgccgc gatccccatt gaaaatggtg gttgaaatca aaatcgagag tcctgcgcga
     2701 ttcgtcggat tccgggccag agcgatcgaa agagtcataa taaaattgca acgtggtgtc
     2761 cgaccggctc gaaaaaacgt gattccatct ccctaggacg ttacctcccg ccagctgatc
     2821 attgacgttt tgatcgccca ccacaggtgg attgatcgag taaatatgga taatcgtgtc
     2881 accggcctgg ccggtgtaca tgtccccttg aacggtaagc gaatctgttg gggaaatctt
     2941 cgtatctgct cgaaagctgc cgtgcaaaag attccaattg tcatggtccg agccaccgtc
     3001 ctcggcgggc aatgaaccgc tattcagata atttatcgcc acacggtagt ccgtgttttt
     3061 gggtaaggag cctccgtatt ggacaaggcc gaaagctttt ccctccgtgc ctccgccacc
     3121 ggaagccagg aacccttttg tgtcagcagc tgttttcgtg acgatgttga tgacgccgtt
     3181 tacggcattc gctccccaga cggtcgcgcc gggtccgcgg atcacttcga tccggccgat
     3241 atcttcgagc ggcacgtttt gggtatccca attcacgccg ccaagcaata cggtgtaaac
     3301 tgcgcggccg tcgattagaa cgagcagctt gtcggaaaac tggccgtcga atccacgcgc
     3361 tgatacagcc caggcgttgg cgttgatctg tgatacgtcc atgcctggaa ccatgcgcag
     3421 caaatccgga atgttagtgg cgccagagcg ttgaatgtct tcatccgtaa tcacgaaaat
     3481 cgcggcggcg acctgcgaca ttttctgatc tgtcttggag accgaagtta ccttaatatt
     3541 catcaaatct tccagagcaa gctgcgagag atcgtttggt gaacccgcga gagcagaatt
     3601 cttcgtcgtc accgctacgg tttgcgccga ggctgtggca gacgtgagcg ataacgccag
     3661 agcaagcacg gtgcatattc gtgttttcga acgtagcatc gaaatcatac ttttccacat
     3721 ggcatgcacc ccgtcacgtg ttgatgaagc atgtgcatga ccaagcttcg gcataaatga
     3781 cttcggcaaa acgcgtcgat tgaataggtt gataaacaga ggaaaaatac gaggtttcaa
     3841 agctgaacaa atcgctgaat tatggtgcaa cccgacactc acaagtgctg ggcgaaataa
     3901 gaaaattcta accgacagag cggagcattc ccttcgcggt cagcttagag tctcggcgcg
     3961 atacaacttc gctgtgttgc agatcgtcgc tatcaccagc atgccagaca ttcccgcggc
     4021 ggcgatgact ccgccagcgt caaacaagcg atagctcaag ccgaatattc gcgcatcggg
     4081 atgaaaccac agcgccatat ttcccgccga cagcaaaagt cgaatctcgg ttggcccaaa
     4141 tttggcgaac gaaagctgaa atgttcccac tgtgtatgtc gccaggtaaa cctgcacaga
     4201 gagcatcaga aacgcaataa acatggcgag cgcaattctc caatcgatca atccggaaag
     4261 tgccagccct cccattagaa agagcccgcc aaacgtgtca accatgtgat caacgtagaa
     4321 accgtagcaa ggacgctgcc ggttgcgtag acgcgcgagc gttccgtcga ggctgtctcc
     4381 aaaccagttc agcgccagaa aaaatgtcgc caggagcaat cccaatcgat tcgtccgcgc
     4441 aaacgcatag ctcgcccccg ccagaaacat agccacaaat cccagcaatg ttagatgatc
     4501 ggaattcatt cgtgcaggta gttttccagc aagccacggc agaactttgc gttccagggg
     4561 cgccgtaaaa ctttcctgca gccggaacgc aggtttaaag gaagcagaaa aggaagcagg
     4621 tcccacggct gcacgcttcg gattttcagc ttgcccgtcc ggcgctataa aaatttgttc
     4681 tggttcattc cacatcgacc gcctccgcaa attagattcc gaattcccta ctgcaaagcg
     4741 tgtgtacctc cacgcagcac cgcagtgcga gtggaagcca gcgtaaattc cagcgactct
     4801 cgtggaatgc tgtctataaa cggagcaatg agccaattga gtcccgcggg aacatcgcgc
     4861 gtaagcgaaa tcgcttcgca ttgcacgtag actcctccgt ctctttccgc aaaccgccaa
     4921 taggaattga gccgccacat aaacccctcg ccctggccct cgggcgacgc ccgctcgccg
     4981 ggttcgtcag gatggctgat ttcagcgatg cgcgtactgt acgattcgct gaatgcccgt
     5041 gtcgcgtcga gcgtgtgata gtgaacttgg tattccgtgt cgagcaccac ggtaacaact
     5101 tttttctttt tcagacgaag atacacttta aagtcgttgc ttttgtgctc gagtattttc
     5161 gatttcacca cctccgggct gtagtaccgg taatgatgat cgtaatcttg aacgagcgcg
     5221 aggacttggc gcagagaaac cccggaaata aaaaccgt
//
LOCUS       DASZMB010000098         8000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1479434, whole genome shotgun sequence.
ACCESSION   DASZMB010000098 DASZMB010000000
VERSION     DASZMB010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8000
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479434"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2797
                     /locus_tag="VG272_04530"
     CDS             <1..2797
                     /locus_tag="VG272_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12980.1"
                     /translation="AKQSKLKDLNEAKAQTYAVTWPVEGDKTELQQFNYEKLNGLFVS
                     IMQSKAKLVAQRGDVDQPAKDAQDKLNAYVKEQLPGLASKDLFTLSQSMKGMDIHIRQ
                     INVNPTGTSLNNLGGAGLVDRCQSCHLGTDPLIVPVTMTLTKADLGLAKSNDAPFTSH
                     PDPDMMKYHPLEKFGCSPCHGGNGRALDTVEKGHGRYEHWLWPLYYKENFNAGCQQCH
                     ASDMVTEHAPVLNRAKQLYREKGCIGCHRFQGFDNQDDELVSARQMISQLENQKEADR
                     LQIAQLNKKGDTAPDNEAANRYYAEATDLTVTSSGMDSKIEQLDLRSHNLLREIKKVG
                     PDLKEVRMKIHKEWIPNWLKHTHEWRPTTKMPQFRLQDDEIQAISAYIWQKALTGPEL
                     PKQAPGNAANGKVLFEERGCEACHSMGEGPNMVGGDFAANLSRVGEKDNYDYLVRWIL
                     NPRTRTRPYSPFEHKDLGPEDYAKHNLPFVFDLDHSRSPNDGHELVVQQMTVMPSLRL
                     TVEDARDIASFLMTQKHADASFPAADFMDNPSPEMLQHGKELIQHYGCAGCHEISGME
                     DEGRVGTELTNEGSKPIERLDFALLTEDAKLGTLPDGKKSPRGDWYDLKGFFENKLTD
                     PAIFDRGKYKPDPMDRLRMPKPNVTKEDIDALTTFLLGSTDPTLPPEYMYKPADQRAA
                     IQKGWWIVTKYNCIGCHQIDIGQKSVLMGLPQYQAENKQNLPPVLTSEGARVSPDWLK
                     AFLNNPSLSTTDMNRNGVRSYLQVRMPTFFLSDDEIRTLVSFFEAISSQPQPYIPQKI
                     EPITTAEKEMARDLFTSSAAPCLKCHATGDPGHDKNAIAPNFLFAKERLQPGWTERWI
                     TNPALIAPGTAMPSGLFKRDGDHWVFSGPLPASFKGYTGDQADLLVRYMFQLTPEEQR
                     ALMGRTPSGGGSGGAGK"
     gene            2867..3667
                     /locus_tag="VG272_04535"
     CDS             2867..3667
                     /locus_tag="VG272_04535"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HWF12981.1"
                     /translation="MKKTKLGMYSLAVGVFAMAITGCGGKKDETSETAAPAANAPAGK
                     SVDAATAGEVTGSVKLEGTAPKMKAINMAAEPSCAKARTSPATSEEVVTGDGGSLANV
                     VVYVKSGLDGYSFPAPTAAAKIEQQGCQYHPHVLAMQVGQNIEVTNADQTTHNIHPIP
                     KDNREWNESQPPGAAPIEKSFTREEVAIPVKCNVHPWMKAYMAVLPNPYFQVTDKDGK
                     FDLKNLPPGTYTLVAWHELYGPSEQTITIGPKESKAVTFTFKAAASGD"
     gene            3903..4820
                     /locus_tag="VG272_04540"
     CDS             3903..4820
                     /locus_tag="VG272_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HWF12982.1"
                     /translation="MNTVYNRALHRYAVLTAFCTFLLLIAGALVTSNDAGLSIPDWPL
                     AYGSLTPPMVGGIRYEFTHRVIATCIGILTIGLAVWLWKAEKREWMRWLGLGALGGVI
                     AQGILGGATVLTFQLPAVSAAHATLAQLFFSTVVSIAVFTSAWWSREQIEADDPGSPR
                     IRTLIVWTLAAVFLQLVLGAAFRHKAFGIIPHLVGAVIVTILIFMTAGALKKRFAGVP
                     ALRSSARYLHILIGVQLLLGVGAYWSRLYGAHFPQPIAIMVTLTVVHTVTGALVLAVT
                     LVTALISYRMIRAGNLIAEPTAASQPVAQ"
     gene            4855..5748
                     /gene="cyoE"
                     /locus_tag="VG272_04545"
     CDS             4855..5748
                     /gene="cyoE"
                     /locus_tag="VG272_04545"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HWF12983.1"
                     /translation="MRESTEATASEASRPALYMALTKPDVSFLVVMTTVAGYALGTHG
                     PLDWLRMAHTVFGTTLIAAGTSAMNHYIERDSDALMRRTASRPLPSGQLHPREAFWFA
                     LSLIGFGTLYLALATNVLATMLGVATSVMYLGAYTPLKKRTMLATFIGSFPGAVPPLI
                     GWAAARGTLNIEAWFLFAMLFLWQFPHFDAIAWVYREDYKRAGIKMIPVADPDGKRTF
                     REILLTAALLVPVSLLPAVTGLAGAHYFFGALILGMMLVEVCLWAAASKTNSRVKWLM
                     HATVLHLPLLLGLLIYDKFTH"
     gene            5782..6027
                     /locus_tag="VG272_04550"
     CDS             5782..6027
                     /locus_tag="VG272_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12984.1"
                     /translation="MNGRENEAELAIMSLVIIVLSALGIVAGFSRGLFGGIDGLLALG
                     ISLMMILVFGILLLVQAKQQGWIGKKHDGGNTPQAVK"
     gene            6145..6585
                     /locus_tag="VG272_04555"
     CDS             6145..6585
                     /locus_tag="VG272_04555"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HWF12985.1"
                     /translation="MISPPALDATLNGASAVLVTLGYVMIRAKRVLAHKACMLGATFT
                     SAIFLVSYVLYHYHLYVIGQGPVHFPGTGAPRYAYLTMLTTHTILAIIIVPLVILSLV
                     RALRSNFPAHKRIARWTLPLWAYVSVTGVLIYVVLYQIYLPPHP"
     gene            6815..7699
                     /locus_tag="VG272_04560"
     CDS             6815..7699
                     /locus_tag="VG272_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12986.1"
                     /translation="MTTITNEAEVSSASSNAPSKPGMAKKWLIAILVLTFAFVLMPFL
                     LWYMTTFTRPLNDSDLASYFADALHPRRSQHALSQIADRITSPNPGTRESAKIWYPEV
                     VRFSEKGSDELRVTAAWVMGQDNQSPDFHAALLKQLADSNPMVQRNAALSLVRFADPS
                     GHALIAGMLEPYTVTAQQAGTLTIRMKPGDAINPGTLLAHIADGSTNSEVRSEVPGTI
                     HQWISADNSAVKAGEPMLLVDPSPEMVWESLRALYLVGQEEDLPAVNLYTAATNGMPT
                     QIQQQATLTAKEITARGN"
BASE COUNT         1891 a         2138 c         2171 g         1800 t
ORIGIN      
        1 cgcgaagcaa tctaaactga aagatttgaa cgaagccaag gcgcaaacct acgcggttac
       61 atggcccgtg gaaggcgaca agactgaact gcagcagttt aattatgaaa aactgaacgg
      121 ccttttcgtt tccatcatgc agtctaaagc caagctggtt gcgcaacgcg gcgatgtcga
      181 tcaacccgca aaagacgcgc aggacaagct gaacgcctat gtgaaagagc agcttcccgg
      241 ccttgcctct aaagatctgt ttactctcag ccaaagcatg aagggcatgg acattcatat
      301 tcgccagatc aatgtgaatc caaccggaac ttcgctcaac aatctgggcg gtgcggggct
      361 cgtcgaccgc tgccaatcgt gccatttggg cacggatccg ctcatcgtcc ccgtaaccat
      421 gacgctgacg aaagcggatc tcggattagc aaaaagtaat gacgcgccgt tcacgagcca
      481 tcccgatccg gacatgatga aatatcatcc gcttgaaaaa ttcggatgct cgccctgcca
      541 tggcggaaat ggccgcgcgc tggacacagt ggaaaaaggg catggccgct acgaacactg
      601 gctatggcct ttgtactata aggaaaattt caacgcgggc tgccagcagt gccacgcttc
      661 ggacatggtg accgaacacg cgcctgtcct gaaccgtgcc aagcagctgt atcgagaaaa
      721 gggctgcatc ggctgccacc gcttccaggg cttcgacaat caggatgacg aactggtcag
      781 cgcgcgccag atgatttctc agcttgaaaa tcaaaaagaa gcggatcgcc tgcaaatcgc
      841 tcagctgaat aaaaagggcg acaccgctcc tgataacgaa gcggcaaacc gctactacgc
      901 ggaagcgacc gacctgaccg tcaccagttc cgggatggac tcaaagattg agcaactcga
      961 tctacgttcg cacaacctgc tccgcgaaat taagaaagtc ggtccggacc tgaaagaagt
     1021 ccggatgaag attcacaaag aatggattcc caactggctg aagcataccc acgaatggcg
     1081 gccgacaaca aagatgccgc aattccggct gcaggatgac gaaatccagg cgatctctgc
     1141 gtacatatgg caaaaggcct tgacgggtcc cgaattacct aaacaagcgc cgggcaatgc
     1201 ggcaaatggc aaagtgctgt tcgaagagcg cggctgtgaa gcgtgccact cgatgggcga
     1261 aggcccgaac atggtgggcg gcgattttgc cgcgaacctc tcacgcgtag gtgaaaaaga
     1321 taattacgat tatctcgtgc gctggatttt gaatccacgc acgcgcacac gtccctacag
     1381 cccgtttgaa cataaagatt tgggtccgga agattacgcc aagcataatc tgccgtttgt
     1441 gttcgatctc gaccattccc gcagccccaa tgacggtcac gaacttgtcg tgcagcaaat
     1501 gactgtcatg cccagccttc gtctcaccgt ggaagacgct cgcgacatcg ccagcttttt
     1561 gatgacccaa aagcatgctg acgcaagctt tcctgccgct gacttcatgg ataatccaag
     1621 cccggaaatg ctgcagcacg gtaaggaact tatccaacat tacggctgcg caggatgtca
     1681 tgaaatttcc ggaatggaag acgaaggccg cgtcggtacg gaactgacga acgagggcag
     1741 caagcccatc gagcgcctgg actttgcgtt gctcacggaa gatgccaagc tcggaacttt
     1801 gccagacgga aagaaatctc cgcgaggcga ttggtatgac ctgaaaggct ttttcgaaaa
     1861 caaactcacc gatcctgcca tcttcgatcg cggcaaatat aaacccgatc cgatggaccg
     1921 tctgcgcatg cccaagccga atgtcaccaa ggaagacatc gatgccttga cgacattcct
     1981 cctcggttcg acagacccga cgctgccgcc ggaatacatg tacaagcctg cggaccagcg
     2041 cgcggccatc caaaaaggct ggtggatcgt cacgaaatat aattgcatcg gctgccacca
     2101 gatcgatatc gggcaaaaat ccgtgctgat gggcttgccg caataccagg cagagaacaa
     2161 acagaatctg ccgccggtgc tgaccagcga aggtgcgcgc gtcagtcctg actggctgaa
     2221 agcgtttctc aacaacccgt cgctcagcac cacggatatg aatcgcaacg gtgtgcgctc
     2281 gtacttgcaa gtgcgcatgc ccacgttctt cctgtccgat gatgaaatcc gcacgctcgt
     2341 ctcgttcttc gaggcgatat cgagccagcc gcaaccttac attccgcaaa agatcgaacc
     2401 cataaccact gcggaaaaag aaatggcacg cgatctattt acaagctccg cggcgccatg
     2461 cctgaagtgc cacgcgacag gcgatcctgg gcacgataaa aacgcaatcg ctcccaattt
     2521 ccttttcgcg aaggaaagat tgcaaccggg gtggaccgaa cgctggatca ccaatcctgc
     2581 tctgattgca ccgggaacgg cgatgccttc cggtttgttc aagcgcgatg gcgatcactg
     2641 ggttttctcc ggaccgcttc cggcaagctt taagggctat acgggagatc aggcggattt
     2701 gctggtgcgc tatatgttcc agctgactcc cgaagagcaa cgcgctctga tgggccggac
     2761 gccatcgggc ggcggctctg gcggtgcagg gaaataatat ttcttgaagt tgtgggtgga
     2821 gagagagatt ctgtagttaa ccaggttaaa caggaggaac cgtttcgtga agaaaaccaa
     2881 actgggtatg tactcgcttg cagttggagt gttcgccatg gcgatcaccg gctgcggcgg
     2941 gaagaaggat gaaacatcgg aaactgccgc acccgctgca aacgcacccg ccggaaaatc
     3001 ggttgatgca gcaacggctg gagaagtcac cggatccgtg aagctggaag gtactgcacc
     3061 taaaatgaag gcaattaata tggcggcaga gccgtcctgc gccaaggcgc gaacgagccc
     3121 ggcgaccagc gaggaagtcg taacaggaga tggcgggtcg ctcgccaatg ttgttgtgta
     3181 cgtgaagagc ggattagatg gttattcttt tccggcgcca accgctgcgg caaaaatcga
     3241 gcagcagggc tgccagtatc atccgcacgt actggccatg caagtaggcc aaaacattga
     3301 agtaaccaac gccgaccaga caacgcacaa cattcacccg atcccgaagg ataaccgtga
     3361 gtggaacgag tctcagccgc ccggcgctgc gccaatcgag aagtccttta cacgcgaaga
     3421 agtcgccatc cccgtgaagt gcaacgtgca cccgtggatg aaggcttaca tggccgttct
     3481 gccaaacccg tatttccagg ttacggacaa agacggcaag ttcgatctaa aaaatcttcc
     3541 accaggaacg tatacgctgg tcgcatggca cgaattgtac ggtccgagcg agcagactat
     3601 cacgatcggt ccaaaggaat ccaaggccgt gacgtttacg ttcaaggcag ctgcttccgg
     3661 agactagatt taatcctctt cgtggattct gagttctgtg cggctgtact tcgaacagct
     3721 gaacacagaa gacgtaaagg aatgcggaga gccacagaat acagcttcag aaaatctggg
     3781 cgcctgggcg aagtaagagt gtgcggtaaa atcttcgcct cggcgttcga aatatcaggg
     3841 tgcgggcgtt ataatcgtac gcgaaacatt tttcataaag aatcgcggag cccgctgtca
     3901 tcatgaatac tgtctacaat agagctttgc accgctatgc ggtcctcact gcattctgca
     3961 cgtttctcct gctaatcgcc ggggctctcg ttacttcaaa tgatgcgggc ctttccattc
     4021 cggattggcc acttgcgtat ggctcgttga cgccgccgat ggtcggtggc atccggtatg
     4081 aatttacgca tcgcgtaatt gcaacctgca ttggcatcct gacgatcgga ctcgcggttt
     4141 ggctatggaa ggccgagaaa cgcgaatgga tgcgctggct gggcttgggg gcccttggcg
     4201 gggtcattgc gcaaggaatc ctgggcggcg caaccgttct gacgtttcaa ttgccggcgg
     4261 tgtccgcggc gcacgcaacg ctggcgcagc tttttttctc cacagtagtg tccatcgccg
     4321 tttttaccag tgcctggtgg agccgcgagc aaattgaggc ggatgatccg ggctcaccgc
     4381 gcattcgaac gctgattgtc tggactcttg cggccgtatt tcttcaactc gtcttaggtg
     4441 ccgcctttcg gcacaaagct tttggaatta ttcctcacct tgtgggcgcg gtgattgtta
     4501 caattttgat ctttatgacg gcgggagctt taaagaagcg ctttgcaggt gtgcctgccc
     4561 ttcgttccag tgcacgctat ttgcacattc tgattggtgt gcagctgttg cttggcgtgg
     4621 gagcgtactg gtcccgcctt tatggcgctc attttccgca gcccatcgca atcatggtga
     4681 cgcttacagt ggtgcacacg gtgaccggcg cactggtttt ggccgtaacg ctggtgacag
     4741 cattaatcag ctatcgtatg atcagggcgg gtaatcttat cgcggaaccc acagcggctt
     4801 cgcagccggt tgcccaatag ggccatttta aagatattga gagaccatta gcggatgaga
     4861 gaatcgactg aagcaactgc gtcggaggca agccgccctg cgctttatat ggctctgacc
     4921 aagcctgacg tttcgttcct ggttgtcatg accaccgtcg caggttacgc gctcgggacc
     4981 catggcccgc tggattggct gcgcatggcc cacacggtat tcggaaccac attaattgcc
     5041 gccggcacat cggccatgaa tcattatatt gaacgcgatt cggacgcctt gatgcgccgc
     5101 actgcgtcgc ggccattacc ctcgggacag ttgcatccgc gcgaagcatt ttggtttgcg
     5161 ctatctctga tcggattcgg cacgctttat ctcgcactgg ccacaaacgt tctcgccaca
     5221 atgttgggcg tagcaacttc cgtgatgtac cttggcgcgt atactccgct aaaaaagcga
     5281 acgatgctgg caacattcat cggttcattt ccaggcgcag tgccgccgct gattggctgg
     5341 gccgccgcgc gcggcacgct aaacattgag gcctggtttt tatttgccat gctattcctc
     5401 tggcagttcc cgcattttga tgcgattgcc tgggtgtacc gcgaggatta caagcgtgcc
     5461 ggaatcaaaa tgattcccgt ggcggatccg gatgggaaac gcacatttcg cgaaattctg
     5521 ctgaccgcgg cgctactcgt ccccgtgagt cttttgcctg cggtcacggg acttgctggg
     5581 gcacattatt ttttcggcgc tttgattttg ggtatgatgc tggtggaagt gtgcttgtgg
     5641 gccgccgctt ctaaaacaaa cagtcgcgta aagtggctca tgcacgcgac cgtgcttcat
     5701 ttgccgttgc tcttgggcct tctgatttac gacaagttta cccactaatt ttcatcgcgc
     5761 acccagcgcc agcgagggtt tatgaacggc cgcgaaaacg aagcagaatt ggcaatcatg
     5821 tccctggtga tcatcgtgct ctcagctttg ggaattgttg ccggtttttc cagaggattg
     5881 ttcggtggga tcgatgggct attagccctg ggcatctcgc tgatgatgat tttagttttc
     5941 gggattttgc tcttggttca ggccaagcaa cagggctgga tcggcaaaaa gcacgacggc
     6001 ggaaacacgc cgcaggccgt caagtaaata gagattcgca aagcagcaaa gacttcagtc
     6061 cgcggatttt gatttttttt aacgtaggaa tacgcgggct gaatttatgt caattgtacg
     6121 attgaaggtg aagggctccg cacgttgatt tcgccacccg cattagacgc aactcttaac
     6181 ggcgcgagcg ccgttctcgt tacgctggga tatgtcatga tccgcgcaaa acgcgttctt
     6241 gcgcataaag catgcatgct cggggcgacc ttcacttcag ccattttttt ggtttcctat
     6301 gtgctatatc attatcacct gtatgttatc gggcaagggc ctgtacattt tccagggacc
     6361 ggcgcgccgc ggtatgccta tctgacgatg cttacgacgc acacgatcct ggcgatcatc
     6421 attgttccgc tggtgatcct gtcgctcgtg cgcgcattac gctccaattt tccggcgcac
     6481 aagcgaattg cgcgatggac tctgccgctt tgggcctacg tctccgtgac cggcgtgctg
     6541 atttacgttg tgttgtacca gatttatttg ccgccccatc cgtagcgaaa cagttgctcg
     6601 atgattgaag tgtttggcgg ctcgagtttc cagtaagtag caagaaatgt tgcgatctgt
     6661 ttttagtgga gcaggctttc gcccgcgggt tttgatgcgc gcacgcgcaa aacttcgcat
     6721 ggagaaaccc gcaccgacca aatcattttc gctgcattga tcaagttgag caggaaagca
     6781 tgtgcaaaga aatctttaga taggtgaacg tgtaatgact accattacga atgaagctga
     6841 agtttcctcc gcgtcttcca atgcgccttc caaaccagga atggccaaga agtggctcat
     6901 cgccattctt gtgctgacgt ttgcttttgt gctgatgccg tttttgctgt ggtacatgac
     6961 gaccttcacg cgtcccctga acgattcgga cctcgcttct tattttgcag acgcattgca
     7021 cccgcgccgc tcccagcacg cgctctcgca aattgcggac agaatcacca gcccaaatcc
     7081 aggaacacgc gagtcggcga aaatctggta tcccgaagtg gtcagatttt cagagaaggg
     7141 aagcgatgaa ttgcgcgtta cggccgcgtg ggtcatgggt caggacaatc aatcgccgga
     7201 ttttcatgcg gcacttttga agcagcttgc ggactctaat ccgatggtgc agcgcaacgc
     7261 ggctctttcg cttgtgcgct tcgccgatcc ttctggacat gcactgattg cgggaatgct
     7321 tgagccctat acggtgacag cgcaacaggc cggtactctt acaattcgta tgaaacccgg
     7381 agatgcaatt aatcccggga cattgctcgc gcacatcgcc gatggatcga cgaacagcga
     7441 agttcgctcc gaagttcccg gaacaattca ccagtggatt tccgctgaca atagcgccgt
     7501 caaagcagga gagccgatgc tgctggtcga tcccagccct gaaatggttt gggaatccct
     7561 gcgcgctctc tatttggttg gacaagagga agatttgccc gccgtaaatc tctacacggc
     7621 cgccaccaat ggaatgccca cgcagattca gcagcaggca acgctgacgg cgaaagaaat
     7681 taccgcgcgg ggaaattagt aatgatgtga ctggtgactg gtgaacaatt tctgatcaga
     7741 aaattctgca aattgcaaga tgaaaccatt cccacgaatc tcaaatcttc aatttgaaat
     7801 taagaagtcc cactcgctat tcaaaaaaaa ccgcgaactg ctcgggcgaa tcaccagtca
     7861 ctagagcgtt tttgatttaa gtgcacacat atcccgaaga agcgaagtag tgtgtgcact
     7921 ctgtcgagga gaccgtgttg aggacttgac cgattttctc ccagagggag tcgacgctgc
     7981 gcgcggcggc ttgccgaagc
//
LOCUS       DASZMB010000099         2253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2013887, whole genome shotgun sequence.
ACCESSION   DASZMB010000099 DASZMB010000000
VERSION     DASZMB010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2013887"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VG272_04565"
     CDS             complement(<1..933)
                     /locus_tag="VG272_04565"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HWF12987.1"
                     /translation="MPVSNRWLMALAGVCLQMALGAAYAWSVFRIPLAKQFGWTISQI
                     TLTFLISWFFLGCAAVVGGLWMKRSQPRVVAMTAGLLWGGGVFLASFSANRLWWLYLT
                     YGVMGGIGLGMGYVVPIAVLVKWFPDRRGLITGIAVAGFGAGSLFSAPAAGWLMLRVG
                     LMPTFAYLGVAYAFIAITAGSFMKNPPAGWKPEGWVPSATQISQRSTRDFTLGQALGT
                     WQWWAISGLMSINTMAGLSIVSQASPIFQELGGATVKTAAGLVGIISIGNGAGRIFWS
                     WVSDLTSRKMAFFAMYLVEVILFWSYHTIHSLPIL"
     gene            complement(1232..1939)
                     /locus_tag="VG272_04570"
     CDS             complement(1232..1939)
                     /locus_tag="VG272_04570"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HWF12988.1"
                     /translation="MDRAAAISELVIANRVVAHLKLVDSFGHVTVRNPENPQRFFMSR
                     ARAPRLVTKEDILEFDLDSAPIDLRGLKPYSERFIHGCLYKSRPDVMAICHNHAHELL
                     PLAVTKTVMRPALHTAAVIGREVPVWDIRDQFGDTNMLVTSIEMGNSLAAAVGNGKAA
                     LMRGHGCVVAGANIQDAVFSAFYLRLNAEIVIKAISMGETITYLSPGEIHRSGELHSQ
                     PAAQGRAWEDWCSQAGV"
BASE COUNT          573 a          656 c          571 g          453 t
ORIGIN      
        1 gagaatcgga agtgaatgaa tcgtgtggta actccagaac agaatgacct cgaccaggta
       61 catcgcaaaa aaggccattt ttctcgacgt cagatcggag acccatgacc agaaaattcg
      121 ccccgcgcca ttgccgatgc tgatgattcc tacaagcccc gccgccgtct taacggtagc
      181 accgcctagc tcctgaaaaa ttggcgaggc ctgggaaaca atcgacaatc cggccattgt
      241 gttaatggac ataaggccgc taatcgccca ccactgccac gtgcccagcg cctgacccag
      301 cgtaaaatct ctcgtcgagc gctgcgaaat ttgcgtggcc gatgggaccc aaccttccgg
      361 cttccagcct gcgggaggat ttttcatgaa cgagccggca gtgatcgcga tgaatgcgta
      421 ggctacgccg agataggcga acgttggcat caggccaacg cgcagcatta gccatccggc
      481 ggcgggcgcg gaaaacagcg agcctgcgcc gaatcccgcg accgcaattc cggtaatgag
      541 gccgcgccgg tctggaaacc atttcaccag caccgcgatc ggaaccacgt agcccatgcc
      601 aagtccgatc ccgcccatca caccatacgt gagatagagc caccacaatc tgttcgcgga
      661 aaagcttgcc agaaaaactc cgccgcccca aagcaaaccc gccgtcatgg ccacaactct
      721 cggttggctg cgcttcatcc acaatccacc cacgaccgcg gcgcacccaa gaaaaaacca
      781 gctgattaaa aatgttaatg tgatttgaga aatggtccag ccgaactgct tcgctagagg
      841 aatgcgaaag acgctccatg cgtaggccgc gcccagcgcc atttgcaggc aaacgccggc
      901 aagggccatc aaccatcgat ttgataccgg catgattcct ccaacggcat catcgcaagc
      961 gggaccgaag ccgcttcggc cactgagaat tatattttgc accaaaaaac gaaaacgcaa
     1021 gtaaaaagcc gcccgtccaa aatacggacg gcctctacaa aagcaaatgc aaatgacacc
     1081 cgattttgta gacgccgctt cgtcccgctc acggagaacg ttctcggtga acctcgcttt
     1141 tcgggacgaa gcgggcgctg tcagtccgcc acgaaaccaa aattataaat cgcgaatcga
     1201 ccgtcgatgc ctgctagttc gatgcgagat tttaaactcc cgcctgcgaa caccagtctt
     1261 cccacgcgcg accttgcgcc gccggctggc tgtgcagttc acccgaacga tggatttctc
     1321 ccggcgaaag atacgtaatt gtctcaccca ttgatatggc cttgatcacg atttctgcgt
     1381 tcaggcgcag gtagaacgcg ctgaacacgg catcctgtat atttgcaccc gccaccacgc
     1441 agccgtgccc tcgcatcagc gccgcttttc cgttaccgac cgcggcagca agagagttgc
     1501 ccatttcaat cgaggttacg agcatattgg tatcgccaaa ctggtcgcga atatcccaca
     1561 caggtacttc gcgcccaatc accgccgccg tgtgcagtgc ggggcgcatc acggttttcg
     1621 tcacggcaag cggaagcaat tcatgggcgt gattatggca gatggccatc acgtcaggac
     1681 gcgatttgta taagcatccg tgaatgaagc gttcggaata cggtttgagt ccgcgcagat
     1741 cgattggcgc gctgtccaaa tcgaattcga gaatgtcttc tttggtgacc aggcgtggcg
     1801 cgcgggcacg ggacatgaaa aaacgctgcg gattttccgg attgcgtacg gtcacgtgcc
     1861 caaaggaatc gacgagcttc aaatgcgcca ccacgcggtt ggcaatcacc aattccgaaa
     1921 tagccgcagc gcgatccacg gaagggctcc tcgtatttca agtttcaagg atgaaataat
     1981 gcaggaagtt tgcacgcaac ggaaagatac tgcaagcgtt taggtagctc tcaggtggca
     2041 taggctttag cctgtggggt tagtgcttgc taggactaaa ccccacaggt gaacaggctg
     2101 cggaaataac tcaacatttt gtcattccga gcgaagcgag gaatctctcc ttctttattt
     2161 tcttatactt aaatcgaaga gagattcctc gcttcgctcg gaatgacaga acaaactact
     2221 ttttccgcaa actgtaaagc cgcattcatt ttg
//
LOCUS       DASZMB010000100         5270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3653184, whole genome shotgun sequence.
ACCESSION   DASZMB010000100 DASZMB010000000
VERSION     DASZMB010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3653184"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2410)
                     /locus_tag="VG272_04575"
     CDS             complement(<1..2410)
                     /locus_tag="VG272_04575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004225.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HWF12989.1"
                     /translation="MYIELHARSAFSFLEGASLPEELAATCAEHGMEAMALLDRDGVY
                     GAPRFYLAAKNTSIRAHIGAEVTSAEGWHYPLLVTSQAGYQNLCRLISKMKLRAPKGK
                     GAVSVSEVAERAGGLICLTGGAEGPLAHALAQGGIENGIACVQKLCDLFGGKNVYLEL
                     QRHLYREEEARNQAAVEIARKMDLPLLATNGVCYARPQRREALDVFTCIRHHKTLATA
                     GRHLSPNSERHLKSPAKMKRLFSDLYEAIANTEELSSRLQFNLKDLGYKFPTYPVPNG
                     QSQMQFLRERCREGLIQRYGLNNERARLQIERELTLIEKLDLAGYFLIVWDIVRFCRE
                     QNILVQGRGSAANSAVCYALGITAVDPVGMDLLFERFLSEERGEWPDIDLDLPSGDQR
                     ERAIQYVYERYGKLGAAMTANVITYRGRSAAREIGKVLSFDVETISRLTKLVSAWEYK
                     DANDTLAHHFRDAGFDLNHPRIRKFFDLCLELQDLPRHLGQHSGGLVVCQGQLDSVVP
                     LEPATMPGRVVVQWDKDDCAAMGIVKVDLLGLGMMAVLEESLQIIKNKYGEEINLGRL
                     PADDPAVYDALQKADTVGMFQIESRAQMSCLPRLMPKCFYDIVVQVAIIRPGPIVGQM
                     VNPYLKRRQGREAVDYLHPSLEPVLKRTLGVPLFQEQLLRMAMIAAGFTGGQAEDLRR
                     AFGFKRSELRMKDIEQKLRAGMERKGIVPEIQDRIVQSITSFALYGFPESHAASFALL
                     AYASAYFKVKYLAAFTCAILNNQPMGFYSPAVLVKDAQRHGLRVRPIDVQVSDWGCAV
                     EHEMD"
     gene            complement(2410..4026)
                     /locus_tag="VG272_04580"
     CDS             complement(2410..4026)
                     /locus_tag="VG272_04580"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HWF12990.1"
                     /translation="MAFASIYVSNFIVQSVVRANPDLRDRMLALVDGDPPLWKVIAAN
                     ESALQAGIELGMGGSQAKQFSKVEVRQRSRSQEKTAHAALLDVGWSVSPRVEDTSLDT
                     IVLDITGLTSLFGSVQNLSTTLTERTASIGLHSNIAVASNMEAAIHAARGFAGITLIP
                     LGEESKRLSSLPVEVLLPSIGILETLHRWGIRTCGALAELPLLQLSERLGQEGIRLHE
                     LARGTFCRSLILMNPETSFQEEMALDYAVTELEPLSFLLGRLLDQLCARLEARSLAAS
                     VFCMQFDIQDATEEEFQIQRKTSNSANDFRIYEKILRLPVPMRDSKTLLKLLRLQLQS
                     DPPHGSIVKLFLTAEPARPRASQNGLFIPNSPDPEKLEVTIARLAALVGDSNVGSPRL
                     INTHRPGEFRMTPFFPATAESILSRKFSSSEQVLASSEIQSRKIRNAIAFRIFRPEIL
                     ANVDSQEGCPCRIYFLGMCGEVIAASGPWRTSGDWWEESGWQQDEWDIEIDFNPSSHE
                     HASRSIMQNGLYRIYYDWTRRRWFVRGRYD"
     gene            complement(4063..4908)
                     /locus_tag="VG272_04585"
     CDS             complement(4063..4908)
                     /locus_tag="VG272_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12991.1"
                     /translation="MPIFAMAANLAQLRSQVELALAGRVTSPFSIPERRIEQVSTGIS
                     EIDAMSGGLPRGALTEIVGPSCSGRTSLLFSAFCARTKLAEACALVDGCDAFDPHSAE
                     AAGIDLKKLLWVRCRTIEQSFRAADFLLAAGGFGFIALDLGDTPRELVHRVPLDTWFR
                     FRRAVEGTPTILLVLEQEPHAKTCASMVLQVEMARSRWNSTRTTDDLDLCRHPFSRLL
                     DASEIRAEMIRSRTHQGIRIDFHDGRDSLTRNDNIPAESERRLHVPAVFKADELFGFK
                     RSWHP"
     gene            5085..>5270
                     /locus_tag="VG272_04590"
     CDS             5085..>5270
                     /locus_tag="VG272_04590"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HWF12992.1"
                     /translation="MHGNLKDLQEDAKNCKACDLWKHATQTVFGEGSPKAKIMMIGEQ
                     PGDQEDKEGRPFVGPAGK"
BASE COUNT         1302 a         1412 c         1363 g         1193 t
ORIGIN      
        1 cgtccatttc atgctcaacg gcgcagcccc agtctgaaac ctgaacgtcg atcggcctca
       61 cgcgcaggcc gtggcgctgc gcatccttca ctagaaccgc gggcgagtag aatcccatgg
      121 gctgattgtt cagaattgcg caggtgaatg ccgccagata tttcactttg aaatatgccg
      181 aagcatacgc gagcagcgcg aagctggcgg cgtgtgattc gggaaatccg tacagcgcga
      241 atgaagtgat cgactgaacg atcctatcct gtatttccgg cacaattcct tttcgttcca
      301 tcccggctcg aagtttctgc tcgatgtcct tcatgcgcaa ctcggaacgc ttaaaaccaa
      361 acgcacggcg caggtcttcc gcctgcccgc ctgtaaatcc ggcggcaatc atcgccatgc
      421 gcaggagttg ttcctgaaag agtggcacac ccaaagtacg cttcagcaca ggttcgagag
      481 aaggatgcag ataatccacg gcttcgcggc cctgacggcg ttttaaataa ggattcacca
      541 tttgcccgac gatcggtccg ggccgaataa tcgccacctg taccacaata tcgtaaaaac
      601 attttggcat caggcgcggc aagcaagaca tctgcgcccg gctttcgatc tgaaacattc
      661 cgactgtgtc ggctttttgc aatgcgtcat ataccgccgg atcgtcggcg ggcaagcgcc
      721 ctaaattaat ttcttcgcca tatttgttct tgatgatttg cagagattct tcgagcacag
      781 ccatcatgcc gagtcccagg agatctactt tcacgatgcc catggcggcg caatcgtctt
      841 tatcccattg cacgacgaca cgcccgggca tggttgccgg ttcgagcggc acaaccgaat
      901 caagttgccc ctggcacacc acaagtccac cggaatgttg gcccaggtga cgtggcaaat
      961 cttgtaattc aagacagaga tcaaagaatt tacgaatccg cggatgattt aaatcaaaac
     1021 ctgcatcgcg aaaatgatgg gccagggtat cattcgcatc tttatattcc caagcgctca
     1081 caagctttgt caggcggcta atggtttcca catcgaatga taaaactttt ccgatctcac
     1141 gagctgccga acgtccacga tacgtgatca cattggcagt catggccgcg cctaatttgc
     1201 cgtagcgttc gtagacatat tgaatggcgc gttctcgttg gtctccactc ggcaaatcca
     1261 gatcgatatc cggccattct ccacgttcct cggaaagaaa tctttcaaag agcaggtcca
     1321 ttcccacggg atcgacagcg gtaatgccca gcgcataaca gactgcgcta ttggcggcgg
     1381 acccgcggcc ttgcaccaaa atgttctgct cgcgacagaa acgcacaatg tcccagacaa
     1441 tcagaaagta acctgcaaga tccagtttct cgatgagggt taattcgcgt tcgatttgaa
     1501 gtcgcgcccg ttcattgttc agtccataac gctggatcaa tccttcccgg cagcgttcgc
     1561 gcaaaaattg catttgcgat tggccattgg gaaccggata agttggaaat ttgtatccca
     1621 aatctttcaa gttgaattgc aggcgtgagg aaagttcttc tgtgttcgca atcgcttcgt
     1681 aaagatcaga aaacaagcgc ttcatttttg cggggctttt caaatgacgc tcggaattgg
     1741 gagacaaatg ccggcccgcg gttgccagag tcttgtgatg gcgaatacag gtaaagacat
     1801 cgagtgcttc ccgtctttgc ggccgggcat aacaaacacc gttggttgcc aacaaaggta
     1861 aatccatttt tcttgcgatc tcgacggcgg cctgattgcg cgcttcttct tcgcgatata
     1921 agtggcgctg caattccaaa taaacattct ttccgccgaa cagatcgcat aatttctgca
     1981 cgcatgcgat tccgttttca attccgccct gcgccagggc atgcgcgagc ggcccttcgg
     2041 ccccgccggt caagcagatg agacctcccg ctcgttcggc aacctctgaa accgaaactg
     2101 cgcctttgcc cttcggggcc ctcaatttca tttttgagat aagccggcaa agattctgat
     2161 atccggcctg tgatgtaacc agtaacgggt aatgccatcc ctctgccgaa gtaacttccg
     2221 cgccaatatg cgcgcgaatc gaagtattct tcgcggccag ataaaaccgc ggtgcgccat
     2281 agactccgtc gcgatcgagc agcgccatgg cctccatccc gtgttctgcg caggtcgcgg
     2341 ccaattcctc tggcagggat gcgccttcga gaaagctgaa tgccgagcgc gcgtgaagct
     2401 ctatgtacat tagtcgtacc ggccgcgcac aaaccagcga cggcgcgtcc aatcgtaata
     2461 aattctgtaa agcccgtttt gcatgatcga tcgagaagca tgctcatgac tggaaggatt
     2521 gaaatcaatt tcgatatccc attcgtcctg ctgccatccg ctttcttccc accaatcgcc
     2581 ggaggttcgc cagggccctg aggctgcaat cacttcaccg cacataccga gaaaataaat
     2641 ccggcagggg catccttcct gcgaatcaac attggccaga atctcgggtc tgaatattcg
     2701 aaaggcgatt gcattgcgga ttttgcgcga ctggatctcg ctgcttgcaa gcacttgttc
     2761 agacgaactg aacttgcgag aaagaatgga ttccgcggtt gcagggaaaa atggcgtcat
     2821 tcgaaattct ccggggcgat gcgtatttat caaccgcggc gagcctacgt tcgaatctcc
     2881 gaccagtgcc gcgagccggg cgatcgtcac ctccagcttt tccgggtccg gagaattcgg
     2941 aataaacaat ccattttggc ttgcacgtgg ccgggctggt tccgcagtga ggaaaagttt
     3001 tacgatcgag ccgtgaggcg gatcggattg caactgcagg cgaagtaatt tcagaagcgt
     3061 cttggaatcc cgcataggca caggtaaacg cagtatcttt tcgtaaatcc tgaaatcatt
     3121 tgccgagttc gaagtttttc tttggatttg aaactcttct tccgtggcgt cctgtatatc
     3181 aaattgcata caaaagaccg aagcggccag ggaacgcgct tctagtcggg cgcaaagttg
     3241 atccagcagg cggccaagca agaaagacaa cggctccagt tcggtaacgg cataatcgag
     3301 cgccatttct tcctggaagg atgtttcggg attcatcagt atgagcgaac ggcaaaaagt
     3361 tccacgcgcc aattcgtgca ggcggattcc ctcctggccc aggcgctccg ataattgcag
     3421 gagaggcaat tccgctaaag cgccgcaggt gcgaatgccc cagcgatgca aagtttcgag
     3481 tattccaata gaaggcaaga ggacttcaac ggggagcgaa ctcaggcgtt ttgattcttc
     3541 tcccaacgga attagagtaa ttccagcaaa gccgcgcgct gcatggatcg cggcttccat
     3601 gttcgacgcc acagcgatgt tggaatgcaa gccaatgctc gccgtgcgct ctgtaagcgt
     3661 agtcgaaaga ttttgtacag acccaaataa tgacgtcaaa cccgtaatat cgagaacgat
     3721 cgtgtccaga gaggtgtctt ccactcgtgg cgaaaccgac caacccacat ccagcaaggc
     3781 tgcatgagcg gttttttcct ggcttcgtga gcgctgccgg acttccactt ttgaaaattg
     3841 ttttgcctgc gatcctccca tgcccagttc gattcctgcc tgcagtgcgg attcatttgc
     3901 cgcaatgact ttccataacg gaggatctcc gtccactagc gcgagcatcc gatcgcgtaa
     3961 atcgggattc gcgcggacga cggattgcac gataaaattg gaaacgtaaa tggaagcaaa
     4021 tgccatgagt ttgaaaatcc cgccaaataa attggcacgg cattagggat gccaactcct
     4081 cttgaaccca aaaagttcat cggccttaaa aacggctggt acgtgcaggc gccgctcaga
     4141 ttccgctgga atattgtcgt ttctggtcag cgaatcgcgt ccatcatgga aatcgatcct
     4201 gataccctga tgcgtacggg aacgaatcat ttcggcgcga atttcagaag catcaaggag
     4261 ccgcgaaaat ggatgacggc aaagatcgag gtcgtcagtg gttcgcgtcg aattccaacg
     4321 cgatcgcgcc atttccactt gcaggaccat ggaagcgcat gttttcgcat ggggctcttg
     4381 ttcgaggacc aataaaattg tcggcgtccc ttcgacggca cggcgaaaac ggaaccaggt
     4441 gtcgagtgga actcgatgca ccaattcgcg aggggtatcg cccagatcga gggcaataaa
     4501 accgaatccg cctgcagcca aaagaaaatc ggcggcacga aagctttgtt cgatggtgcg
     4561 gcagcgcacc cacagcaatt tcttaaggtc aattccggct gcttcggcgg agtgtggatc
     4621 gaaagcatcg cagccatcaa ccagcgcgca ggcttccgca agcttcgttc gtgcgcaaaa
     4681 ggcagaaaat agtagactgg tgcggcctga acaagaaggc ccaacaattt ccgtgagagc
     4741 gcctcggggt agccctccac tcatcgcgtc aatttcagaa atgcccgtgg agacctgctc
     4801 aattctgcgc tctggaattg aaaagggaga ggtgacacgc ccggccaggg cgagttcaac
     4861 ttgagagcgc aactgagcaa ggtttgcagc catagcgaat atgggcaagg gggaaaggac
     4921 gtttcagtat tcgccttttc ttcgccatcg agattttccc caagttcggc aggattgtca
     4981 atcccagctc tcacaatttt tatttttaaa tgcggacaca cgttcgtaga tcgcatctaa
     5041 atatgtatgg cgaagcgccc cgcgaaggac tccgcgaacc tgttatgcac gggaatttaa
     5101 aagatctgca ggaagacgca aaaaattgca aggcgtgcga tttatggaag catgccacgc
     5161 aaaccgtttt cggcgaaggc tcgccgaaag cgaaaattat gatgattggc gagcagccgg
     5221 gcgatcaaga agacaaggag ggccggccat tcgttggacc agcagggaaa
//
LOCUS       DASZMB010000101         5586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1482702, whole genome shotgun sequence.
ACCESSION   DASZMB010000101 DASZMB010000000
VERSION     DASZMB010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1482702"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VG272_04595"
     CDS             <1..528
                     /locus_tag="VG272_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HWF12993.1"
                     /translation="IVTLDVTKLRRMVGMVFQKPNPFPKSIYENVAYGLRINGHKGRL
                     DDAVEKSLHRAALWDEVKDKLHKSAYALSGGQQQRLCIARALAVDPEVLLLDEPCSAL
                     DPVNTAKIEELLFELKPTCTLVIVTHNMQQAARVSDSTAFMLNGELIEFSPTAHMFTT
                     PSDPRTEAYITGRFG"
     gene            584..1255
                     /gene="phoU"
                     /locus_tag="VG272_04600"
     CDS             584..1255
                     /gene="phoU"
                     /locus_tag="VG272_04600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HWF12994.1"
                     /translation="MERHFERSLEELKERLLWMGSLAERAVHQAVHAVLDADEKLAQV
                     VLKEENAINELQIEIDDRVVQLLALQQLMAADLRFVLAIARINNDLERIGDQAVNIAH
                     SATRIVRHPRVKPYVDLPRMGELAEKMMRDSLDSLVRRDIELARSVLSRDDLVDQLRD
                     QIFRELLSYMMGDSSVVFPAFELILVAKNLERIADHATNVAEDVIYMVAGSDVRHPAV
                     DRNPA"
     gene            1342..1905
                     /locus_tag="VG272_04605"
     CDS             1342..1905
                     /locus_tag="VG272_04605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWF12995.1"
                     /translation="MSQTTNTQLTAEIPGGDDNEPILEGHALEKFYLQPDGGRIQIIS
                     PTDIKIMPGKILALLGPSGCGKSTLLRMLTGLSAPSAGEVFYHGRKLTEDPPNVGIVF
                     QSFALFPWLTVLQNVEAPLEARGVAASERHERAMRIIDSVGLDGFENAYPKELSGGMK
                     QRVGFARALVIEPEVYSWTNHSPRSTC"
     gene            1872..2705
                     /locus_tag="VG272_04610"
     CDS             1872..2705
                     /locus_tag="VG272_04610"
                     /inference="COORDINATES: protein motif:HMM:NF021350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-associated domain-containing protein"
                     /protein_id="HWF12996.1"
                     /translation="MDEPFSALDVLTAETLRGELMELWNDRKIPTRAIFIVTHNIEEA
                     VVLADRVIVLASHPARIREDFEITLAQPRSRKSEPFESLVDHIYRVLTQPELEHEMPA
                     EMKSAAATPSVPAPAPRYIMLPHVRAGGIAGLLEILVDYGGRQDLHRVSHQLMLESDD
                     LLPIVEACVLLGFTRVEHGDAIITDLGSEFAAGDIQRRKDIFRETLLAHVPVLQQMAS
                     ALQAKKDGTMLLDFFHDLLDEHFTDDEVRRQLDTAIQWGRYAELFDYDSVTGRLSRPK
                     P"
     gene            2728..4482
                     /locus_tag="VG272_04615"
     CDS             2728..4482
                     /locus_tag="VG272_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWF12997.1"
                     /translation="MKLDPVLHRSTIFRIISERAKWKSPLWADVVVFAGLLAIVYALF
                     VSARVWFEPFAPVANISSSPLALPLYAAYSLVRISIAYIFSLLFALGYGMAAAKSERA
                     AKILLPLLDILQSIPVLSFLPGVMLAMVALFPHRQLGLELGSILLIFTGQAWNIAFSF
                     YASLKGIPRELDEAARLYRFSAWQRFTELELPFAAIGLVWNSMMSVAGGWFFLMACEM
                     FVLGTRDFRLPGLGSYLQVAASAGDTRAVLWGMAAMIAAIILLDQLIWRPAIAWSDKF
                     KFETVEGIAPQSFVLKVLRRSGILASFYRTAIYPVEERVTRAFVIKKNFGVRQSSQKS
                     SLNKWIFRVFAAIAFVAVAWLAYRALETISQLGQRQLLFLARDAAFTFLRVNASLILG
                     ALWTVPVGVWIGTTPRVAKIAQPLVQIAASVPATALFPVLLLLLIRFGGGMGTAAMAL
                     MLLGTQWYILFNVIAGAMAIPTDLKEAANVFRFTRLERWRRLILPAIFPYLVTGMLTA
                     SGGAWNASIVAEYFHFQGQTLSVRGLGAAISRATDSGNLPVLLTATILMSVIVVMVNR
                     TLWQRLYRLAATRFKLET"
     gene            5170..>5586
                     /locus_tag="VG272_04620"
     CDS             5170..>5586
                     /locus_tag="VG272_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12998.1"
                     /translation="MTRIEPGTVLTVRTNEGIDVEHRDNRVYQGTVDQDVRGENGRLA
                     IPRGSQVELIVRVARDNDLVLDLDSVTVNGQRYGLETDPNRQESERDNSVVGQIMGAI
                     QGGQARGRAVHIPRDSRLDFRIARPLVVGVADRGEDR"
BASE COUNT         1153 a         1493 c         1618 g         1322 t
ORIGIN      
        1 atcgtgacgc tcgacgtcac gaaattgcgc cgcatggtgg gcatggtgtt ccagaagccc
       61 aacccgtttc ccaagtccat ctacgaaaat gtggcctacg gtttgcgaat caatggacac
      121 aagggacgcc tggacgatgc ggtggaaaaa agtctgcacc gcgccgcttt gtgggacgaa
      181 gtgaaggaca agctgcacaa gtccgcctat gcgctttccg gcggacagca gcaacggctt
      241 tgtatcgcgc gagccttggc cgtggatcct gaagttttgc tgctcgacga accgtgctcc
      301 gcgcttgatc ccgtgaatac ggcgaagatt gaagagttgc tgttcgagct gaagccgacc
      361 tgcacgctgg tgatcgtgac acataacatg cagcaggcgg cgcgcgtaag cgattcgaca
      421 gccttcatgc tgaatggcga gctgatcgaa ttttcgccaa ccgcgcacat gttcactacg
      481 ccttcggatc ctcggacgga agcgtacatc acgggccgct tcggatgagc cttgtggcaa
      541 cgccgggagg ggattagtct ttagcgtgga gaggacgtag cgaatggaac gccattttga
      601 acggtcattg gaagagttga aagagcgact gctctggatg ggcagtcttg ccgagcgagc
      661 tgtgcaccag gcggttcatg cagtgctgga tgcggatgaa aagctcgccc aggtggtgct
      721 caaggaagag aatgcgatca acgaactgca gattgaaatc gatgaccgcg tagtacaact
      781 cctcgcgctg cagcagctga tggccgcgga tttgcggttt gttctggcca tcgcgcgcat
      841 caacaatgac ctcgaaagaa tcggcgatca ggcagtgaac atcgcgcatt cggcaacgcg
      901 cattgtgcgc cacccgcgcg tgaagcccta tgtcgacctg ccgcgcatgg gcgagcttgc
      961 cgaaaaaatg atgcgcgaca gcctcgattc tcttgtgcgc cgcgacattg aacttgcgcg
     1021 ctcagtgctt tcgcgcgatg acctggtgga ccagctccgc gaccagattt ttcgcgagtt
     1081 gctcagttac atgatgggcg attcgtccgt cgtgttcccg gcatttgaat taatccttgt
     1141 ggctaaaaac ctggagcgca ttgccgacca tgcgaccaac gtcgccgaag acgtaattta
     1201 catggtggcg ggcagcgacg tgcgtcatcc tgccgtcgat cgcaatcctg cctgagcata
     1261 ctcgatcggg acttccgagc cgaacccgtc tcgctcaatt cacatgtttc gaccatccct
     1321 taaatcgttc ataatgcgtc cgtgtcacaa accaccaata cgcaattgac ggccgagatt
     1381 cccggcggag atgataacga gccgattctc gaagggcacg cgctggaaaa gttttatttg
     1441 cagccggatg gcggccgcat ccaaattatt tctcccacgg acatcaaaat catgccggga
     1501 aaaatcctgg cgctgctcgg gccatcaggc tgcggcaagt cgaccctctt gcgaatgctg
     1561 accgggttgt cggccccctc cgcaggcgaa gtgttctatc acggccgcaa gctcacggag
     1621 gatccgccca atgtgggtat cgtttttcag agcttcgcgc tatttccctg gctcacggtt
     1681 ctgcaaaatg tggaagcgcc gctcgaagcg cgcggcgtgg ccgcctcgga acgccacgag
     1741 cgcgccatgc gcatcattga ctccgtgggt ctcgacggat ttgagaacgc ctatcccaag
     1801 gaactttccg gcgggatgaa acagcgcgta ggtttcgcgc gagctcttgt gattgagccg
     1861 gaggtttatt catggacgaa ccattctccg cgctcgacgt gttgacggcg gaaacgctgc
     1921 gcggcgaatt aatggaattg tggaatgacc ggaaaattcc gacgcgggca atttttattg
     1981 tcacgcacaa catcgaagaa gcggtcgtgc tggccgatcg agtgattgta ttggccagcc
     2041 acccggcgcg cattcgtgag gattttgaaa ttacgctcgc gcagccgcgc agccgtaagt
     2101 ccgagccatt tgaatcgctg gtcgatcaca tttatcgcgt tctgacgcag cccgagctcg
     2161 agcatgaaat gcccgcggaa atgaaatccg cggccgcgac acccagcgtt cccgcacctg
     2221 caccgcgtta catcatgttg cctcatgtgc gggccggagg tattgccgga ttgctcgaaa
     2281 ttctggtgga ttacggcggg cgccaggatt tgcatcgcgt ctcgcaccag ttaatgctcg
     2341 agtctgacga tctgcttccc attgtggaag cctgcgtatt gcttggtttc acgcgcgtcg
     2401 aacacggcga cgcgatcatt acggatctgg gcagtgaatt tgccgccgga gatattcaga
     2461 gacgcaagga catattccgc gaaaccctgc ttgcgcatgt tccggtgctc caacagatgg
     2521 catccgcact acaagccaag aaagacggta cgatgcttct cgattttttt catgacttgc
     2581 tcgacgaaca tttcaccgat gacgaagttc gccgccagct cgataccgcg atccaatggg
     2641 gccgctacgc cgagcttttt gactatgact ccgtaaccgg acggctaagc cgtcccaagc
     2701 cgtgaagcag cgcaggcctt cccgcccatg aaactcgatc ccgtccttca tcgctctact
     2761 atttttcgca ttatttccga acgcgcaaaa tggaaaagcc cgctttgggc cgatgtggtt
     2821 gtttttgcgg gactattggc gattgtctac gcgctattcg tttccgcgcg tgtgtggttt
     2881 gagccttttg ctccggtagc caacatttct tcaagtcctc ttgcgctgcc gctgtatgcc
     2941 gcctactcac tggtgcgaat ttcgattgct tatattttct cgcttctttt tgcgctgggc
     3001 tacggcatgg ctgccgcgaa gtccgaacgt gcggcaaaaa ttcttctgcc cttgctcgac
     3061 attttgcaat cgatcccggt gctgagtttt cttcccggag tgatgctggc catggtggcg
     3121 ctttttccgc acaggcagct tggcctggag ctgggttcca tcctgctgat tttcaccggg
     3181 caggcgtgga acatcgcatt cagtttttac gcatcgctca aaggtatccc acgcgaattg
     3241 gatgaggcgg cgcggttgta ccgcttcagc gcgtggcaga gattcacgga attggaattg
     3301 ccgtttgcgg ccatcggact cgtgtggaat tcgatgatgt ccgtggctgg cggctggttt
     3361 ttcctgatgg cctgcgaaat gtttgtgctc ggcacgcgcg atttccggct gccgggtctc
     3421 gggtcttact tgcaggtggc ggcaagcgcg ggcgataccc gcgccgttct gtgggggatg
     3481 gcggcgatga tcgccgccat tattctcctc gatcaactta tctggcggcc cgcgattgcg
     3541 tggtcggaca aattcaagtt tgaaaccgtc gaaggcatcg ccccgcaatc tttcgtgcta
     3601 aaggtcttgc gcaggtccgg aatccttgcg tctttttatc gcaccgcgat atatccggtg
     3661 gaagagcgtg taacacgcgc atttgtgata aagaaaaatt tcggcgtgag gcaatcgtct
     3721 caaaaaagta gtttgaataa atggatcttc cgcgtgttcg ctgcaatcgc ctttgtagct
     3781 gtggcgtggc tggcctatcg agcgcttgaa acgatttcgc agcttgggca acggcagctt
     3841 ctttttctcg cgcgcgatgc ggcgttcacg ttcctacgtg tgaacgcgtc actcatcctt
     3901 ggcgctctct ggactgttcc cgtaggcgtc tggatcggta ctacgccgcg agtcgcgaaa
     3961 attgcgcagc ctctggtgca gattgccgcc tcggttccag cgactgcctt gtttccagtg
     4021 cttctgttgc tgttgattcg atttggcgga ggcatgggaa ccgccgcgat ggcgctgatg
     4081 ctgctcggca cgcagtggta cattttgttt aatgtgatcg ccggtgcgat ggcgatccct
     4141 acggatttga aggaagccgc gaatgtgttc cggtttacgc gattggaacg ctggcggcgt
     4201 ctgatcttgc cagctatctt tccgtacctg gtgacgggca tgctgaccgc ctcgggtggc
     4261 gcgtggaatg cgagcattgt cgcagagtat tttcattttc agggacaaac gctttccgtg
     4321 cgagggctgg gcgcggccat tagccgcgcg actgattctg gaaatttgcc tgtcctcctg
     4381 accgcgacca ttctcatgtc cgtaatcgtt gtgatggtga accgtacact atggcaaagg
     4441 ctttatcgtt tggcggcaac tcgttttaag ctggagacct aatttgtcag gcgggcaatt
     4501 ttcttccagt ggtggtgttt ttttcggcga agccagtttt tcttcctgcg agtttaggag
     4561 ctgggacggt tgcccgggcg ttgaattaga acttgggtgc cccgtcctac gcattttgta
     4621 gggcggggct ttttcgtctg tccctctacg tatcgcaagt tgaagcaaaa gccccgccct
     4681 tgaaaagcgt caagaacggg gcacccgaag tgcaaaaaca taactctaag tggacgtact
     4741 ggactagtat cccattatgc agcggagaaa gttctgtgca atgcgaaagg ccgggctacc
     4801 cgtccgaagt agctaacagc tgacaaaatg cgacgggata agccaacatc aatatcgcag
     4861 tactgtggcg tgctggggcg cgatcacgga gaggtccgag tcatccctca accaaatact
     4921 gtttctccct tatggatgta aaagcgcagt ctgcgtaacc ttgaaagacc agccttccta
     4981 cggtgtttaa gtcgcaagta atcgtgggga aggagattag aaatgtttaa tcgacaaagt
     5041 gatcgccgcc cgtcccaaat ctgggcattt gcgccggcgt tggcagcctg cctggttcta
     5101 tgttttgggc cgacgctttc cgcacaaggt caagatcggg accaagatcg agacagagaa
     5161 catgacagaa tgactaggat tgagcccggc acggtgctca cggtacgcac aaacgaggga
     5221 atcgacgttg agcatagaga caatcgagtt tatcagggca ctgtagacca ggatgtgcga
     5281 ggagaaaatg gacgactagc catccccaga ggctcgcaag tcgaattgat cgttcgagtc
     5341 gctcgcgata acgatctggt cctcgacctt gactctgtta cagtgaatgg acagcggtac
     5401 gggctggaga ccgatcccaa tcggcaagag tcggaacgcg ataacagtgt cgttgggcag
     5461 ataatgggtg ccattcaagg cggccaggct cgcggccgcg cagttcatat tcccagagac
     5521 agcagattgg atttccgaat tgcacgtccg ctggtggtgg gagtagctga tcgcggagaa
     5581 gatcgc
//
LOCUS       DASZMB010000102         5710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3656772, whole genome shotgun sequence.
ACCESSION   DASZMB010000102 DASZMB010000000
VERSION     DASZMB010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3656772"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VG272_04625"
     CDS             <1..253
                     /locus_tag="VG272_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF12999.1"
                     /translation="KRIYFSGATGTLAVFHEDDPNHFVLLGKVPTGAIAKSGIWIPEL
                     KRYYAAVPRHLVELLPTTQYNYHDWITEEAHLMVFEALP"
     gene            498..1373
                     /locus_tag="VG272_04630"
     CDS             498..1373
                     /locus_tag="VG272_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13000.1"
                     /translation="MKTPFTIWTRSALLACTLTAAIAVPTWSDTLVLRSGATYSGTLT
                     GVNGNWIVFRDGRGIERHYMARDVESVQFGDAPNQSRGGRPGYDQSRVDDRNYRGNND
                     DQGRGNRDNDCGYDQDRMERVVLPDGTELAILTNERIDSRDVVEGQTFSAEINEDIRN
                     SDGSIAIPRGSDASLVTRRVEGNGDLTLDVQSIVVGGCRYRVSTADQELENHKDGVGA
                     NKRTGQFMGGGAVLGAVIGAIAGGGKGAAIGAVAGAGAGAGAQIITQGKEVHVPAESV
                     LRFRLDRPLSLHLWS"
     gene            1627..4032
                     /locus_tag="VG272_04635"
     CDS             1627..4032
                     /locus_tag="VG272_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HWF13001.1"
                     /translation="MSRAKRVFVLLAVMLIAAETGHAQQTRFHLQEATVDDIHRAIRS
                     GQITCQGLVRLYLNRAKAYNGVSNMLVTKDGAAIPTPPGVIRAGSPLKFPTETISIST
                     LLPNFDQYAGPPIEFGRMEPTASDPTVQQQFGMTVGIPNAGQLNALGTLNIRGERSAT
                     CKGDRDRRPSDGPLPPGAPAVCEEFRKQPDALERAAELDAQYGKNPDLAKLPMYCIVF
                     SFKDSYDTKDMRSTGGGDANYDIDFPSRDQTLVAQLRQKGAIIYAKTANTEYNGRPVP
                     AIRGGEGTSRGINRPTKVFVSVQGYQRSTWAGNPSDAYDTTRAASLGSSSGSGVSVSS
                     NLVTCSLCEETSMSCRGPSNHNGVALILPHKSLISFLGGAIGADIYLDRAGVMCRTVK
                     DAAKVLDALKDPANGYYDPRDIFSTVPRSSVLDKPYSASVVSGARGSLRGMRIGIVRE
                     SMLTFPGVKADEPISQAADKEIKAILGDYLGATLVESVDPLWPDDPKIENMKPSYTDA
                     LAQLAPILFPDIFYRLDRNGKPQFPEFAAKIKPIEFAPGKTFGSGTMKPIDYFLELTE
                     GRVPIPKNLNIRAIEEVVESNAFTLQFNQYASRRAADWKARGFMETLVDFPSLNARSK
                     FWGDDERAAFKNWEEMYDMRNGLGDRQGIDEHIMMRELLRRVEMKVIMENHLDAVVRL
                     HYSLPPGKIGLAGQPEPQGDIRGELRMGPFSGVTEVLIPAGYVRTVYDPVFVLSADKK
                     RYVPTANNTPTALPAPGLPFSLVFRAEPGKEDVILKVASAYEAASKRRVAPSEFGPLP
                     GEP"
     gene            4134..4640
                     /locus_tag="VG272_04640"
     CDS             4134..4640
                     /locus_tag="VG272_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13002.1"
                     /translation="MRRPQLTLKLIAFGIFMGMLATSLMAGQGTQPTGKLVLFADLNA
                     FGPASNPDSCIARNRYKKGETVGFRLYAVDGGTNAAEASAEVTVHISFGGKTFDFPAL
                     YRGVPHKTPSGGNMPVHAGMWTTKWVVPDDAPTGIINYSATAHDKFGRTAEWKPMGGE
                     PSLLTIVQ"
     gene            4664..>5710
                     /locus_tag="VG272_04645"
     CDS             4664..>5710
                     /locus_tag="VG272_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13003.1"
                     /translation="MLRGGTIAFFTLSFFFCTQLACSLASAGKATQATAKPRADSFQK
                     LELTYDKPVVGARIRATAAGLPPGKIVELKWVTVTGGWVIEDYYYFRGKKFSESTLSL
                     GKFPVNADGRLDATFAIPEDYGGVHNVIATIDGQPVAQNGVEVTQSFEMSPLSGPIGT
                     PIELRVKGLGWRTMENTWVVNWDNRGVGFVTAVSTRGSAVARFRAAGPVGDHPIKIYT
                     GWQGQSYLNFEQAPNAYLPRPDFTFRTTAGRSPTPEAYGDEYQPYPLPESEIHIPNGK
                     LSVSPTQGRVGAHTTLSGEGFPANSTLDVFWQTYVGSRVSGNGFAPQEKSIAKIKVGT
                     DGKIEFPLTIPLDLG"
BASE COUNT         1346 a         1514 c         1592 g         1258 t
ORIGIN      
        1 caaacggatc tatttttcgg gcgccacagg cacactcgcc gtttttcatg aagacgatcc
       61 caatcatttc gtgttgctcg gcaaagttcc aacgggagcg attgcgaagt ctggaatctg
      121 gattccggaa ctgaagcgat attatgcggc agttcccaga cacctcgttg agctgctgcc
      181 cacaacgcaa tacaattatc acgattggat caccgaagaa gcccacttga tggtttttga
      241 agcgctgcct taattagtgt tattgcgaag aaagctgccc tctccgtccc gaaatgcttg
      301 ggacggagag ccctctacga agagcgacaa cctttgtcag gggcttcgca gaggtagttt
      361 tctttgagta aacaggaatt ccgctatccc aataatttga tgtacaccct agaactcggg
      421 cctgattgtt tggttgcgcc gatgggcgag ctcgatcaaa atctcagtca tcggagattg
      481 ctcggaggga aataaatatg aagacacctt tcacaatttg gacaagatca gcgctgcttg
      541 cctgcacgct gaccgcggcg attgccgtcc ccacctggag cgatacgctg gtgcttcgga
      601 gtggagctac gtattcgggc actttgaccg gagtgaatgg caactggatc gttttcagag
      661 atggccgcgg catcgagcgc cattacatgg cgcgcgatgt ggaatctgtg caattcggcg
      721 atgcgcccaa tcaatcgcgc ggagggcgac cgggttacga tcaatctcgc gtcgatgacc
      781 gcaattatcg cggcaacaac gacgatcagg gaagagggaa ccgcgataac gattgcggtt
      841 acgatcagga taggatggag cgagtcgtgc tccccgatgg aactgagttg gctatattga
      901 cgaatgaacg catcgattct cgcgacgtgg tggaaggaca gactttttca gcggaaataa
      961 acgaagacat tcgcaactcc gacggctcga ttgcgattcc tcgaggctca gacgcgagcc
     1021 tcgtcactcg acgcgtggaa ggcaacggcg accttacgct ggatgtgcaa tccatcgttg
     1081 ttggaggatg ccgctaccgc gtaagcacgg cggatcaaga actggaaaat cataaagatg
     1141 gcgtgggagc taacaagcgc actggacaat ttatgggtgg tggcgctgtg ctcggcgcag
     1201 tgatcggagc cattgctggc ggcggaaaag gtgctgcgat cggcgctgtc gcaggcgcag
     1261 gggcgggcgc gggagcgcag atcatcacgc aaggcaaaga agtgcacgtt ccagcggaat
     1321 ccgtgcttcg ctttcggctg gatcgccctt tgtcacttca tttgtggtcg tgatttgagt
     1381 tcgattgcca tgaaggcata acattcgccg caaataaagc gcctgaaaac attagtggaa
     1441 attatcatgg cttggcatca gtcaggcgta tgatttgatt ttcatggttc acgaagcgct
     1501 aaattcctta caaattactg ctcgaatttc gttttttgtc tgtccgtatt tttttgaatt
     1561 tccatgaaac gcgcggggtg aaaatctgct ctaagtacca aacgcaacta aaattccgga
     1621 ggctctatgt cgagggctaa gagagttttt gttctgcttg ccgtgatgct gatcgccgct
     1681 gagacggggc atgcgcaaca aactcgcttt catcttcagg aagcgaccgt agacgacata
     1741 catcgggcga ttcggagtgg gcagatcact tgccagggat tggtgcggct gtacctcaat
     1801 cgagccaagg cctacaacgg cgtcagcaac atgctggtca cgaaagatgg agcggctata
     1861 ccaacgccgc caggagtgat tcgcgcaggc tcaccactta aatttcctac ggaaacaatt
     1921 tccatttcta ctttgttgcc caatttcgat cagtacgcag ggccgccgat tgagttcggc
     1981 agaatggaac cgaccgcgtc cgatccgact gtgcagcagc aattcggaat gaccgtcggc
     2041 attcccaacg ccggtcaact caacgcgctc ggcacgctga atattcgagg cgaacgctcg
     2101 gcgacctgca aaggggatcg cgaccggcgc ccatcggatg ggccattgcc gccaggcgca
     2161 ccggccgtgt gcgaagaatt ccgcaagcaa ccggatgcac tcgagcgcgc ggccgaactc
     2221 gacgcgcaat acggcaagaa tcctgatttg gcgaagctgc cgatgtactg catcgttttt
     2281 tctttcaaag attcgtatga cacgaaggac atgcgcagca cgggcggcgg ggatgccaac
     2341 tacgatatcg acttcccatc gcgcgatcag acgcttgtcg cgcagcttcg gcaaaaaggc
     2401 gccatcattt atgccaagac ggcgaatacg gaatataatg gccgcccggt tccggctatt
     2461 cgcggcggcg aaggcacctc gcgcggaatt aaccggccaa caaaagtgtt tgtttcggtc
     2521 cagggttacc agcgcagcac gtgggcggga aatccaagcg atgcgtatga caccactcgc
     2581 gccgcatctc tcggctccag ttccggttca ggcgtttccg tcagctccaa tctcgtgacg
     2641 tgcagcctgt gcgaggaaac gagtatgtct tgccgcggtc cttccaatca caatggagtg
     2701 gcgctgattc tgcctcataa atcgctcatt tcatttctcg gcggcgcgat tggtgcggac
     2761 atttatctgg atcgcgcagg cgtgatgtgc cgcacggtga aagacgcagc caaagttttg
     2821 gacgcgctca aggatccagc caacggatac tacgatccac gcgatatctt ctccactgtt
     2881 cccagatcga gcgtgttgga caaaccgtat tccgccagcg tggtttcggg ggcacgcggt
     2941 tcgctgcgcg gaatgcgcat cggaattgtc cgtgaatcaa tgctgacttt cccgggcgtg
     3001 aaggccgatg agccgatttc gcaagcggcc gacaaggaaa tcaaggccat tctgggagac
     3061 tacttgggcg cgacgctcgt cgagtcggtc gatccccttt ggccggacga ccccaaaatt
     3121 gaaaacatga agccgtccta cacggatgcg ctggcgcaac ttgcgcccat actgttcccc
     3181 gatatttttt accgccttga ccgaaatgga aaaccgcaat ttcccgaatt cgcggcgaaa
     3241 atcaagccca tagaattcgc tccgggaaaa actttcggtt ctggcaccat gaagccgatt
     3301 gattattttc tagaattaac agaaggccgg gtgccgatcc ccaaaaattt aaatattcgc
     3361 gccatcgaag aggtggtcga atccaacgcg ttcacgctgc agttcaatca atatgcttcc
     3421 cggcgcgcag ccgattggaa ggcgcgcggt ttcatggaaa ctttggtcga tttcccttcc
     3481 ctcaatgcac gatcgaaatt ctggggcgac gatgaacgcg cggccttcaa gaattgggaa
     3541 gagatgtacg atatgcgtaa cggactaggg gaccggcaag gaatcgacga gcacatcatg
     3601 atgcgcgaat tgctgcggcg tgtggaaatg aaggtgatca tggaaaacca tctcgacgcc
     3661 gtggtccgtc tccactattc gctgccacca ggaaaaatag gcctggctgg acaaccagaa
     3721 ccgcagggcg acattcgcgg tgagctgcgc atgggcccat tctccggcgt gacggaagtt
     3781 ctgattcctg cgggatatgt gcgaaccgtt tatgatccag tttttgtgtt gagcgccgat
     3841 aagaagcgat acgttcccac cgccaacaac acgcctacgg ctttgccggc gccgggtctg
     3901 ccgttctctc ttgtttttcg ggcggagcct ggaaaggaag acgtcatttt gaaagttgcc
     3961 tcggcctacg aggcagcttc caaacgccgc gttgctccat cagaatttgg accgctgccg
     4021 ggcgaacctt agaccggcca ggagacattt aaaatccgcg gttgctaccc caacgcccta
     4081 aactgaagca aaatttctag acaacgatgg aaagttgcac tatggttgca cgcatgagaa
     4141 gaccccaact tacccttaaa ctgatcgcct tcggtatttt catgggaatg ctggccacat
     4201 cgctcatggc cgggcaggga acccagccca cgggcaagct ggttttgttc gccgacctga
     4261 acgccttcgg tcctgcttcc aatcctgaca gttgcatcgc aagaaatcgg tacaagaagg
     4321 gcgaaacagt gggcttccgc ctctatgcgg tggacggtgg aacgaacgca gccgaagcat
     4381 cggccgaagt tacggtgcat atcagttttg gcggaaagac gttcgatttc ccggcactgt
     4441 atcgtggagt gcctcacaaa actccttcag gagggaacat gccagtccat gctggaatgt
     4501 ggaccaccaa gtgggtcgtt ccggatgatg cgccaaccgg cataattaat tattcggcca
     4561 cggcacatga taaatttggc cggacagccg aatggaaacc gatgggcggc gagccatctc
     4621 ttctaaccat cgttcaatag cggaggaatt tctgtccagg catttgctcc gtggcgggac
     4681 tatcgcattt tttactctga gtttcttctt ttgcacgcaa ctcgcttgct cacttgcatc
     4741 ggctggtaag gcaacgcaag caactgcaaa accgcgcgcc gattccttcc aaaagctcga
     4801 actgacctac gacaaacctg tggttggcgc aagaatccgg gcgaccgccg cgggattgcc
     4861 tcccggcaaa attgtcgaac ttaaatgggt cacggtcacc ggcggttggg ttattgaaga
     4921 ttattactat ttccgcggaa agaaattttc tgagtcaact ttatcgctcg gaaaatttcc
     4981 agtgaatgcg gatggccgac tggacgcgac ctttgcgatt cccgaagatt atggcggcgt
     5041 tcataacgtg atcgcgacga tcgatgggca gccggtcgcg caaaatggcg tggaagttac
     5101 gcaaagtttt gaaatgtctc ctctatccgg cccaatcggg acgccgattg aattacgcgt
     5161 caaaggtctc ggctggcgaa ccatggaaaa tacgtgggtc gtaaactggg acaatcgtgg
     5221 ggtcggattt gtcacggcgg taagcacgcg cggttctgcc gttgcgcgct ttcgcgcggc
     5281 tggtcccgtt ggcgaccatc ctataaaaat ctacacggga tggcaggggc aaagttatct
     5341 gaatttcgag caggcgccga acgcgtatct gccacgccca gatttcactt tccgcacgac
     5401 tgctggcagg tcacccacgc cagaagccta tggggacgaa taccaaccct atcccttgcc
     5461 ggaatcggaa atccacattc caaatggaaa attaagcgtc agcccaacac aagggcgcgt
     5521 cggggctcac acaacgctga gcggcgaagg cttcccggcc aattcgacac tcgatgtttt
     5581 ttggcagacc tatgttggca gtcgcgtgag tggcaacgga tttgcgcccc aggaaaagtc
     5641 gatcgcaaaa ataaaagttg gaacggacgg gaagatcgaa ttcccgctga cgatcccatt
     5701 ggacctcggc
//
LOCUS       DASZMB010000103        15495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2852518, whole genome shotgun sequence.
ACCESSION   DASZMB010000103 DASZMB010000000
VERSION     DASZMB010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 15495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15495
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2852518"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1014)
                     /locus_tag="VG272_04650"
     CDS             complement(136..1014)
                     /locus_tag="VG272_04650"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HWF13004.1"
                     /translation="MTQKFKVGLIQMSCSLDPGENLAKVEARVRAAAQRGAQIVCTQE
                     LFRSQYFCREENADLFELAEPIPGPSSEALSKVARDLKIVIVGSLFERRAAGIYHNTA
                     VILDADGSLLGKYRKMHIPDDPLYYEKFYFTPGDLGFLNYDTRYGRIGVLVCWDQWYP
                     EAARLSALGGANILFYPTAIGWHPAEKQEFGAAQLDAWRTIQRSHAIANGVFVAAVNR
                     VGFEGSPESGLEFWGHSFVSDPFGRVLSEASTEKEEILIVECDPKLSEETRRNWPFLR
                     DRRIDAYSPILKRWNE"
     gene            1244..1579
                     /locus_tag="VG272_04655"
     CDS             1244..1579
                     /locus_tag="VG272_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13005.1"
                     /translation="MPLTLSAVYIFLKKHYKNEPAKPIGWPLILGVWVLGGPFMTFGA
                     SFSGGGFNGPDGISGGIKLMLVTLIPIFTIDMATYDGSLGALFIASFGGLLLWILLVA
                     TAKKDRGFH"
     gene            complement(1667..2224)
                     /locus_tag="VG272_04660"
     CDS             complement(1667..2224)
                     /locus_tag="VG272_04660"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF013979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HWF13006.1"
                     /translation="MAFVAKKIYLTKGVGKHRERLSSFELALRSAGIAACNIVRVSSI
                     FPPNCKLISRAEGLKHLKPGQVAFTVISENSTREAHRLIAAGIGLALPADKSMYGYLS
                     EHHSFGETEEVAGEYTEELAAEMLATTLNVEFDPDLSWDEKKEVYRISNKIVRTMNIT
                     QSAVGDKRGLWTTVLAAAIMIGEDD"
     gene            complement(2564..3097)
                     /locus_tag="VG272_04665"
     CDS             complement(2564..3097)
                     /locus_tag="VG272_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWF13007.1"
                     /translation="MLDQQVADLDLLVETLEASGVAPDSDALLKMAEILGKLFDVKTD
                     EVAVLKVVPKYRSLKFVIPEKLTPVGTIPLTSTTALAARSARDRKPELANNFSTARHA
                     NVFEAVPLGRNPSELIQKIMSAPILDGTRVHGVVQISRKAATLAQAGPDFTQKDLRKL
                     VSLSPALDRFLKLCKLD"
     gene            3444..4163
                     /locus_tag="VG272_04670"
     CDS             3444..4163
                     /locus_tag="VG272_04670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HWF13008.1"
                     /translation="MTETTADTKAAPNRLVLLWNSVIGKKVVMAVTGAVLVLFVISHM
                     VGNLKIFSGPEEINAYSRFLRTVAMPELGYGQLLWIVRGILLLSVILHIVAATQLTII
                     NREARPVGYGTKKNVETSWGALTMRWGGVLLAIFIVFHLFHLTAGMVGFQPGQFEHLA
                     VYQNVVAAFAVVPISLFYIIAMFALCLHLDHGIWSGLQTLGWVNLTNTKMLRAISRVI
                     ALIIFVGFISVPISVLAGWVR"
     gene            4242..6152
                     /locus_tag="VG272_04675"
     CDS             4242..6152
                     /locus_tag="VG272_04675"
                     /inference="COORDINATES: protein motif:HMM:NF005749.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HWF13009.1"
                     /translation="MLDSHIPSGPIEQKWEKARFEMKLVNPANRRKHTVIIVGSGLAG
                     ASAAASLGQQGYNVHCFCFQDSPRRAHSIAAQGGINAAKNYRNDGDSIHRLFYDTVKG
                     GDFRSREANVFRLAQISVNIIDQCVAQGVPFAREYGGLLDTRSFGGVQVSRTFYARGQ
                     TGQQLLLGAYQGLEEQIAVGSVKMHTRCEMLDVVVINGRARGIVTRNLLTGAIETFAA
                     DCVVLATGGYGNVFYLSTNAKGCNTTAIWRAHRRGAYFGNPCFTQIHPTCIPVSNENQ
                     SKLTLMSESLRNDGRVWVPKKVGDTRPPSQIPEEERDYYLERRYPSFGNLVPRDVASR
                     NAKEVCDEGRGVGPGGQGVYLDFADAIRRMGESVVREKYGNLFDMYEEITGENAYKVP
                     MRIYPAVHYTMGGLWVDYQLMSTIPGMFVAGEANFSDHGANRLGASALMQGLADGYFI
                     LPYTIPNYIASNSLEKVDTSHPEFEVVKNEVNQRVNKLLSINGNRTVDSFHKELGKLV
                     WGNCGMARSADGLRENLVKIPQLREEFWSDLKIIGSGAELNQSLEKAGRVADFLELGE
                     LMCLDALEREESCGGHFRVESQTPDGEALRDDDRFSFVSAWEWKGEGDQWELHKEPLT
                     FEHVHLAQRSYK"
     gene            6156..6908
                     /locus_tag="VG272_04680"
     CDS             6156..6908
                     /locus_tag="VG272_04680"
                     /inference="COORDINATES: protein motif:HMM:NF005746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HWF13010.1"
                     /translation="MNLTIKVWRQQDSKSAGKFMTYPVQNVTPDMSFLEMLDLLNEDL
                     QAKGEAPVAFDHDCREGICGMCGFLINGVAHGGHRGTTVCQLSMRFFKDSDSLTLEPW
                     RAKAFPVLRDLAVDRSAFDRIIQAGGFITVPTGVAPDARAVLVPKEAADRAMDAAACI
                     GCGACVAACPNASAMLFTAAKIVHLNSLPQGQPQRDERTTDMVAQMSEEHFGSCTNHG
                     ECEAVCPKKIPLEFIGRMNRDLIHATLHRRGE"
     gene            7143..8288
                     /locus_tag="VG272_04685"
     CDS             7143..8288
                     /locus_tag="VG272_04685"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HWF13011.1"
                     /translation="MNTEGFSSRARQIHFSSLVFDTHVDTAQRLLFDHFDLAKRDTEG
                     CVDIPRMREGGIGAIFFALWVPVEIMGGAATRRARELLDATLNQIKIHDSELSLATTV
                     EEVRSARARGKIAVLLGIEGGHAIDSDLAILREFYAQGVRYMTLTHNAATEWADSSND
                     APRHNGLTELGKQVIFEMNRLGMVVDVSHVSDATFYDVLETSRAPVIASHSCCRALCD
                     APRNLDDVMIKALADKGGVIHITFHDSFLSQEYADSKRRLSQETSEREEYINKKCGDN
                     EAQSLMEWQRWSNELVRGGKLPQVNWEKIVEHIDHAVQLVGAHHVGLGSDFDGAFMPL
                     GMEDTSKFPFITEGLLARGYAESHIQKILGENTLRVMADVARASESV"
     gene            8574..9344
                     /locus_tag="VG272_04690"
     CDS             8574..9344
                     /locus_tag="VG272_04690"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWF13012.1"
                     /translation="MRYTSKIAILACACSIFSLAAAAQTPAPAKKPTTPGKAPAAKAP
                     ASKGPFDPALLHPAALTAKAPAEYEVKFVTTQGEFTVKVTRAWAPNGADRFYNLVRHH
                     YYDGASFFRVLSGFMAQFGLSAYPEVSNAWEKANIKDDSLVQHNQRGFITFATAGPNT
                     RTTQVFINYGNNLALDKMGFTPFGQVTEGMEVVDKLYSGYGEGAPDGKGPDQSKVGNQ
                     GRVYLMKNFPLLDSIKTATIVPPAAPGSAPAAAPAPAK"
     gene            complement(9531..10472)
                     /gene="mdh"
                     /locus_tag="VG272_04695"
     CDS             complement(9531..10472)
                     /gene="mdh"
                     /locus_tag="VG272_04695"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:TIGR01763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HWF13013.1"
                     /translation="MRKKVTVVGGGFVGSTTAQRIVDLELADVVLTDILDGPPAGKAL
                     DMVESTPITGSDARVTGVSTASGDYKETANSDVVVVTAGFPRKPGMSRDDLLKANYDV
                     VKAVVEQIVKFSPNAILILVTNPLDAMVQAAFKVSGFSKNRVMGMAGVLDSARMSSFV
                     AMELGVSVENVNSFVLGGHGDDMVPLPRYSTVAGIPLPDLLPADRIAAIVDRTRKGGA
                     EIVNLLKTGSAYYAPSAAVVEMVEAILKDKKKILPCAAYLEGEYGINGLYVGVPVKLG
                     ARGIEQIIEIKLTAEEKAALDKSAASVRELVTILGVA"
     gene            complement(10551..11972)
                     /locus_tag="VG272_04700"
     CDS             complement(10551..11972)
                     /locus_tag="VG272_04700"
                     /inference="COORDINATES: protein motif:HMM:NF005610.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HWF13014.1"
                     /translation="MKPSYNGRAIPSDGQAIQYVNGKFVIPDNPIIPYIEGDGTGRDI
                     WKASQRVFDAAVQIVYGGKRKISWFEVFAGEKAFNEFKKWLPDETVEAVRDFRISIKG
                     PLTTPVGGGIRSLNVALRQILDLYACVRPVRYFQGVASPVKHPERMDVVIYRENTEDV
                     YIGIEWKSGTPEVKKLIEFLNKDMLGGGPKQIRADSSIAIKPISPFGTKRLMRRAIEY
                     ALENNKRVVTIMHKGNIQKFTEGSFRDWGYELAVEEFRAPTVTERETWILGNKDKDAN
                     LTIEQNAALIEPGMEQGTEAFRKSVCDEVKSVLEKIYPSHGQGKWKQKLLINDRIADS
                     IFQQVITRADEYQVIAAPNLNGDYISDACAAQVGGLGMAPGANIGDGYGCFEATHGTA
                     PKYADKNVINPSSVMLSGVMMFDFMGWKEVGKLIESGIERTVGQKKVTYDLERMMEGA
                     TKLGTAEFASAIIENMQSMAVTK"
     gene            complement(12125..13438)
                     /locus_tag="VG272_04705"
     CDS             complement(12125..13438)
                     /locus_tag="VG272_04705"
                     /inference="COORDINATES: protein motif:HMM:NF019606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HWF13015.1"
                     /translation="MRKSAGIALPKGKLGVLTVGMGAVATTFMAGVDLIRRKKSQPIG
                     SLTQMGTIRLGKRTEGRAPAIKDFVPIASIEDIVFGSWDIFPDSAYEAAKNAAVLSNE
                     HLAECKKFLSKIKPMKAAFDQNYVKKLTGTHVKKGRTKYDLALQIKADIQSFQKKNKV
                     SRTVVIWCGSTEIFLKPHAVYETPEKFEAAMKENHPAIAPSMLYAWAAITSGVPFANG
                     APNLTVDVPAMVTLSKQHGVPICGKDFKTGQTLMKTILAPGFKARLLGMNGWFSTNIL
                     GNRDGEVLDDPESFKTKEESKLGVLEYILQPKLYPTLYGQIHHKVRINYYPPRGDNKE
                     GWDNIDIFGWLGYQMQIKVDFLCRDSILAAPIVLDLVLFLDLAKRAGMRGIQEWLSFY
                     FKSPMCAPELYPEHDLFIQSMKLKNTLRWMMGEDLITHLGQEYYD"
     gene            complement(13911..>15495)
                     /gene="polX"
                     /locus_tag="VG272_04710"
     CDS             complement(13911..>15495)
                     /gene="polX"
                     /locus_tag="VG272_04710"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HWF13016.1"
                     /translation="AKLLKKYPPTILNLLELQSLGPKKVAFLWKAFKAGTVDEVEKLA
                     REGKLRDLPGFGEKTEENILKAISVYKKSAGRFHIDEAEQTANILVSHIRAFGKPIEE
                     VTPAGSLRRCKETIGDLDLLVTLAHGKSTVETVESISQHILAFPGIDQTLAHGENKVS
                     FLLRNALQVDVRILDKESYGAALLYFTGSKEHNVVLRGRANKMGLTLNEYALATLEGE
                     HPVARATEKEIYAKLKLDYVPPELRENTGEIDAAAEHHLPHLIELRDILGDMQMHTTA
                     SDGKNSIEEMALAAKDLGYKYISLTDHSKAVTVANGLDEKRTLEQIKKIQAANSKDLG
                     IRVLSSSEVDVLKNGKLDMDDEVLAQLDVVLVSIHSYMTMERAEMTDRILAAIENPYT
                     QIVGHPTGRLVLRRDAYAYDMERILDAAKKNNVVMECNASPERLDLKDIHLRMAKERG
                     VKIVISTDAHSTHGLQTMRYGVQMARRGWIEKKDVINTLGLDEMLSGLRAKPGSAPQK
                     TVQKSAASKKLSAKARNVN"
BASE COUNT         3435 a         3978 c         4271 g         3811 t
ORIGIN      
        1 gaatcatcaa ttcgctcccc agctggtttt tggcaacttc ccgcctgttt ccgggtggcg
       61 gttttcactc gtttaccctg agtgtcgaag ggtgccagcc acaatggtgg cttgttgaac
      121 ttaagtatgt aggtttcact cgttccatcg cttgagaatg ggggagtagg cgtcgatgcg
      181 gcggtcgcgc aggaacggcc agttgcggcg cgtttcttcg cttaacttag gatcgcattc
      241 gacaatcaaa atttcttctt tctctgtgga ggcttcggag agtacacgtc cgaaaggatc
      301 tgacacgaaa gagtgtcccc agaattcgag gccgctttcg ggagagcctt cgaagccgac
      361 gcggtttacg gctgcgacga acacgccatt ggcgatggca tgcgagcgct gaatggtgcg
      421 ccacgcgtcg agctgcgccg cgccgaattc ctgtttctcc gcaggatgcc agccaatcgc
      481 agtgggataa aaaagaatgt ttgcgccgcc gagtgctgaa agccgcgccg cttccggata
      541 ccactggtcc caacagacga gcacgccgat gcggccgtat cgagtgtcat aattcagaaa
      601 acccaggtcg cccggagtga aatagaattt ttcgtagtag agcggatcgt ccgggatgtg
      661 catcttgcga tattttccga ggagtgatcc gtccgcgtcc aaaattacgg ctgtgttgtg
      721 gtagatgcct gctgcgcggc gctcgaagag ggaaccgaca attacaattt ttaaatcgcg
      781 cgcgactttt gacagcgcct cggaggacgg ccccggaatt ggctcggcca gctcaaacaa
      841 atccgcgttt tcttcgcggc agaaatattg cgagcggaac aattcctgcg tgcagacgat
      901 ttgcgccccg cgctgtgccg ctgcgcggac acgcgcttcc accttggcga gattctcccc
      961 cggatcgagg gagcaactca tctgaatgag tccaacttta aatttttgtg tcacgcagga
     1021 atcatagcat tatccattta tttttgaagg gtgtacattt ctggagacgg accagtgttc
     1081 atgccgggaa agtctatcgg aggcaaccat gagtggccat acggcgcatg tactaaaatg
     1141 catgattttg ggagccgtta gtttctggat cccagacgcc atatggcacg caatcatagg
     1201 ctctaagttt ggtgggcgag atgctatcgc ccttacggga ctcatgcctc tcacattgtc
     1261 cgcagtctat atcttcctga agaaacacta taagaacgaa ccagcgaagc ccattgggtg
     1321 gccgttgatt ctaggcgtgt gggtgctcgg aggtccattc atgacgttcg gagcaagttt
     1381 ctcggggggt ggtttcaatg gcccagatgg tatctctggt ggaataaaac taatgttggt
     1441 cacccttatc cccattttca cgattgacat ggctacctac gatgggagct tgggcgctct
     1501 attcatcgct tctttcggag gattacttct ctggatactg ttagtcgcga cggccaagaa
     1561 agatcgtgga tttcactaaa atctttcgct aacgatttcg gaactaaatc cggatggcac
     1621 aacccaatgg ggtaatttct cgcgatacga gggtacgaga tatttcttag tcgtcttcgc
     1681 cgatcatgat ggcggcggct aaaactgtgg tccacaggcc gcgcttatcg cctacggcgg
     1741 actgggtgat gttcatggtg cggacaattt tgttggagat tcgataaact tctttttttt
     1801 catcccagga caaatcgggg tcgaattcga cgttgagtgt cgtcgctaac atttcggcgg
     1861 cgagctcttc ggtgtattcg ccggcaactt cttcggtttc gccgaaggag tggtgctcgg
     1921 acagatagcc gtacatggat ttatcggcgg ggagagccag gccgatgccc gcggcgatca
     1981 ggcggtgcgc ctcgcgcgtg gaattctcgc tgatgacggt gaacgcgact tgcccgggct
     2041 tcaagtgttt caagccctcg gcgcgcgaga tcagcttgca attgggaggg aagatcgacg
     2101 atacgcgcac gatgttgcac gcggcgatgc ccgcgctgcg caatgcaagc tcaaagctgg
     2161 aaagccgttc tcgatgtttg ccaacacctt ttgtcaggta aatctttttc gcgacgaacg
     2221 ccatgagggt gcgggagtgc gctgcctcct actacaaaat gacttctgaa aattttttgc
     2281 cactttttag tgcgtgcgaa aaagtaacaa gaacgaacaa ctgtgtcaat tactttcccc
     2341 gttgaagata cagattttag gcatcttgtg attgctggag aagtgtggga ggcgtttttg
     2401 aatggaaatg tgcgattcgg cgcgactcgg attagataaa tgcaatttaa ggaaaatttg
     2461 cgcaaatatt taggagattt gcaattcggg agttcaacgg ttgaaagttt ggagcgaggg
     2521 aatgcccatc tgaaggtggg cgctacagaa ttctttcacg ggatcagtct agcttgcaga
     2581 gtttcagaaa tcggtcgagc gcgggtgaca gggaaacgag tttgcgcaaa tctttttgcg
     2641 tgaaatcggg gcctgcttgc gcgagcgtcg cggctttgcg ggagatttgt acgactccat
     2701 ggacgcgtgt gccgtccagg attggagcgc tcataatttt ctggatcagt tcgctcggat
     2761 tgcgccccag cggaacagct tcaaaaacat tggcgtgccg tgccgtactg aaattgttgg
     2821 cgagttccgg tttgcggtcc cgcgcgctgc gcgcggcaag cgctgttgtg ctcgtcaaag
     2881 gaatggtgcc cacgggagtg agcttttccg gaatgacgaa cttcaagctc ctgtatttcg
     2941 gaacgacttt caggacagca acttcatcgg tttttacgtc gaaaagtttt ccgagaattt
     3001 ccgccatctt gagcagtgca tcggaatcgg gagcaacgcc gcttgcttcc agcgtttcga
     3061 ctaataaatc caaatccgca acttgctgat ccagcatgaa aaagtctccc gtgaggtagc
     3121 caagtcgatt atatccgatt gatgagcaag gaaattccaa ctgttctcgc ctgtgtagct
     3181 aatccaatat catcgccatt gaaaaaccgg cgagaattac ctatactgag ttttctagtt
     3241 catcggagcc ggaatgtctg cgatcggagt ggattttcga catcgcctaa ttttcccacg
     3301 ggcctatccc ttcaagaaga ttcagaaatt gccatagaat taaatgtggt tccaagcagg
     3361 gaggcacagg gtgtgccggt acgagcagag tgcgccagaa cggttcggct taaagccatg
     3421 gaaataaatt caggagttca gcaatgacag aaactacggc ggacacaaaa gcggccccca
     3481 accgccttgt acttctttgg aatagcgtga tcggcaagaa agtcgtcatg gccgtaaccg
     3541 gcgcggtact cgtccttttt gtgatctcgc acatggttgg caatctaaaa atattttccg
     3601 gcccggaaga gatcaacgcg tattcgcgct tcctccgtac ggttgcaatg cccgagcttg
     3661 gctatgggca acttctgtgg attgtgcgcg ggattttgct gctgagcgtg attttgcaca
     3721 ttgtcgcggc cacgcaactg acgatcataa atcgcgaggc gcgtccggta ggttacggaa
     3781 caaagaagaa cgtggagacg tcgtggggcg cgttaaccat gcgctggggc ggcgtgctgc
     3841 tcgccatttt catcgtgttc catttgtttc atttgacggc cggcatggtt ggatttcagc
     3901 ccggccagtt tgaacatttg gctgtctatc aaaacgttgt tgccgctttt gcggttgtgc
     3961 caatttcctt gttttacatt atcgcgatgt tcgccctgtg cctgcatctc gatcatggta
     4021 tctggagcgg gttgcagacg ctgggttggg ttaatttgac caacaccaag atgctgcgag
     4081 ccatttctag agtcattgcg ctgattattt ttgttggatt tatctcggtg ccgatatcgg
     4141 tgctcgcggg ctgggtgcgc taaaagtagg ggcacgacat gtcgtgcccc tacacgacat
     4201 gttgtgcctc tacaattgtg acaccgccac actggagaga aatgctcgat tcacatattc
     4261 cgtccggtcc tattgaacaa aagtgggaaa aagcccgctt tgagatgaag ctggtcaatc
     4321 ccgccaaccg ccgcaagcac acagtgatta tcgtgggctc gggactcgcg ggcgcttcgg
     4381 ctgccgcgtc gcttggccag cagggctata acgtgcattg cttctgcttt caggacagcc
     4441 cgcgccgtgc tcactcgatc gccgcgcagg gcggcatcaa tgcggccaaa aattaccgca
     4501 atgacggcga cagcatccac cgcctgtttt atgacacggt gaagggcgga gacttccgct
     4561 cgcgcgaggc caacgttttt cgcctggcgc agatcagcgt gaatattatc gaccagtgcg
     4621 tggcgcaggg cgttccgttc gcacgtgaat acggcggact gctggacacg cggtcgttcg
     4681 gcggggttca ggtttcgcgc acattttatg ctcgtgggca aacggggcag cagcttcttc
     4741 tgggtgcgta ccagggactc gaagagcaaa tcgctgttgg cagcgtcaaa atgcacacgc
     4801 gctgcgagat gctcgatgtt gtcgtgatta atgggcgggc tcgtggcatc gtgacacgca
     4861 atcttttgac tggtgcgatt gaaacttttg ccgcggattg cgtggtcctt gcgacaggcg
     4921 gatatggcaa tgttttctat ctctccacca acgcgaaggg ctgcaatacc accgccatct
     4981 ggcgcgcgca ccggcgcggc gcatatttcg gcaacccctg cttcacacaa attcacccga
     5041 cttgcattcc ggtgagtaat gaaaatcagt ccaagctcac gctaatgtcc gaatcgctca
     5101 ggaatgacgg gcgcgtttgg gttccgaaga aggttggtga tacgaggccg ccttcgcaga
     5161 ttccggaaga agagcgagac tattatctgg agcggcgcta tccgagtttt ggaaaccttg
     5221 tgccccgtga cgttgcttca cgcaacgcca aggaagtgtg cgacgaaggg cgcggcgtcg
     5281 ggcccggcgg ccagggtgtt tacctggact ttgcggacgc gattcgccgg atgggcgaat
     5341 ccgtggttcg cgaaaaatac ggaaacctct tcgatatgta cgaagaaatc actggggaga
     5401 atgcatacaa ggttcccatg cgcatttatc ccgcggtcca ctacacgatg ggcggcctgt
     5461 gggttgatta ccaactcatg tccacgattc ccgggatgtt cgtcgccggg gaagcgaatt
     5521 tctcggacca tggcgccaat cgactgggcg caagcgcgct gatgcagggg cttgcggacg
     5581 ggtatttcat cctcccgtac acgattccca actacattgc ttccaactcg ctggaaaagg
     5641 tcgatacgtc tcacccggaa tttgaagtgg tgaagaacga agtcaaccag cgcgttaaca
     5701 aactgctgag catcaacggc aacagaaccg ttgattcctt ccacaaggaa ttgggcaaac
     5761 tcgtttgggg aaattgcggt atggctcgct cggcggatgg attgagggaa aacctggtca
     5821 agattccgca gttgcgcgag gaattctgga gcgacctgaa gattattggc tcgggagcgg
     5881 aactcaacca gtcgctcgaa aaggccgggc gcgttgcgga ttttctggaa cttggcgaac
     5941 taatgtgtct cgatgcgctc gagcgggagg aatcctgcgg cggacatttt cgcgtcgaga
     6001 gccagacacc ggatggcgaa gcattgcgcg acgacgatcg attctcgttc gtgtcggcat
     6061 gggaatggaa gggagaaggc gatcagtggg agttgcataa ggaaccgctg accttcgagc
     6121 acgttcacct tgcccagagg agttacaagt agcccatgaa cctcacaatc aaagtttggc
     6181 ggcagcagga ttccaagtcg gcagggaagt ttatgaccta tccggtgcag aacgtcacgc
     6241 ccgatatgtc cttcctcgaa atgctggact tgctcaatga agatctccag gcaaagggcg
     6301 aagctcccgt tgcattcgat cacgattgcc gcgagggaat ttgcggcatg tgcggattcc
     6361 tgatcaatgg cgtggcgcat ggcggacatc gcggcacgac ggtatgccag ctttccatga
     6421 gatttttcaa ggatagcgat tcgttgacgc tggaaccgtg gcgcgcgaaa gcatttccgg
     6481 tcctgcgcga tcttgccgtt gatcgttccg catttgaccg catcattcag gccggcggct
     6541 tcattaccgt gcctacgggc gttgcgccgg atgcgagagc tgttctcgtg cccaaggaag
     6601 cggcggatcg cgcgatggac gctgcggctt gcattggatg cggcgcatgc gtggcggctt
     6661 gcccgaatgc ttcggccatg ctgttcacgg ccgcgaaaat cgtgcatctt aattcgctgc
     6721 cgcaagggca accgcagcgt gacgagcgca ccacggatat ggttgcgcaa atgtccgaag
     6781 agcattttgg aagctgcacg aatcatggcg agtgcgaagc tgtatgcccg aagaaaattc
     6841 cgctggagtt tatcggtcgc atgaaccgcg acttgattca tgcaacgctg catcggcgcg
     6901 gggaatagct cgttttttga gtatagtttt tgtggcgctg gcgggatgcc agcgctacaa
     6961 aatatcaccg aagggcttct aacgtgagga taattcgaat ctggtatgcg aagaattctt
     7021 ggtaacgaca cacttacggt tatggagaag tggcataggc ttcagcgtgt ggaatttggt
     7081 ccttgcacgc acgaaacccc acaggctaaa gcctatgtca caaaaacccg cgtcccaaat
     7141 tcatgaacac cgaaggcttt tcttcgcgag cacggcaaat tcatttttcc agtttggtgt
     7201 ttgataccca cgtcgatacg gcccagcggc tccttttcga tcattttgat ttggctaagc
     7261 gtgacacaga aggctgcgtc gacattccca gaatgcgtga gggcggtatt ggcgcgatct
     7321 tttttgcgct atgggttccg gtggagatta tgggcggtgc cgccacccgg cgcgcacgcg
     7381 aactgctcga tgccacactc aatcaaatta aaattcacga ttccgaattg tcgctagcta
     7441 ccactgtcga agaagttcgc tccgcgcggg cacgcggcaa aatcgcggtt cttctaggga
     7501 ttgaaggtgg acacgccatc gatagcgacc tggcgatctt gcgcgaattt tacgcgcagg
     7561 gcgtgcgtta catgacgcta acgcataacg cagccacgga atgggcggac tcctcgaatg
     7621 atgcgccgag gcacaatgga ttgacggagc ttggcaagca agtaatcttc gaaatgaacc
     7681 gattagggat ggtcgtcgat gtatctcatg tatctgacgc tactttttac gatgtgctgg
     7741 agacttcacg cgcgccggtg atagcttcgc attcctgctg ccgcgcgttg tgcgatgcgc
     7801 cgcgaaatct ggatgacgta atgatcaaag ctctcgccga taaaggcggc gtgattcata
     7861 tcacgtttca cgattcattc ctcagccagg aatatgccga ttcaaaacga aggctttcgc
     7921 aggagacttc cgagcgtgag gaatatataa ataaaaaatg tggagacaat gaagcgcaga
     7981 gtttgatgga atggcagcgc tggagtaatg aattggttcg cggtgggaaa ctgccacagg
     8041 taaattggga aaagatcgtc gagcatatcg atcacgcagt ccaactggtg ggcgcgcatc
     8101 acgtcggcct cggttcggat ttcgacggcg cgttcatgcc ccttgggatg gaggacacct
     8161 caaagtttcc attcattacg gagggtctgc tcgcgcgcgg atacgcggaa tcccacatcc
     8221 agaaaattct gggagagaat acgctgcgag tgatggccga tgtggcgcgg gcttcagagt
     8281 ctgtgtgaga actgcaaaat tgggacttca gcggctaaag ccgcattcat tttgcagaac
     8341 ttatgtcgtg gctaaagcca cgacccacaa agattctgca gttctcacac agactcttca
     8401 gtctgcgggg ggtagttctc gcgaggtcca aattccgcag gctgaagacc acgccactaa
     8461 aacctgcgcc acgaaacctt aatatccttt atgattccga aaaatcgcgg ctagtgttac
     8521 caagggaatc gcggcataat atgcggcgtt ccaactaaaa tggagaaatg aaaatgcgct
     8581 acacttctaa aattgcaatt cttgcttgtg catgttctat tttttcgctc gcggcggcgg
     8641 cgcaaacgcc tgcgccggca aagaaaccga cgacgcccgg gaaagcgccg gctgccaagg
     8701 ctccggcgag caaagggcct ttcgatccgg cgttgctgca tcccgcggcc ttgaccgcta
     8761 aagcgccggc agaatacgaa gtgaaattcg tgaccacgca gggcgaattt accgtgaagg
     8821 taacgcgtgc gtgggcgccc aacggcgcgg accgctttta taaccttgtc cggcatcatt
     8881 actacgatgg cgcaagtttc ttccgcgtac tttccggatt catggcgcaa tttgggctga
     8941 gcgcctatcc cgaagtttct aacgcctggg aaaaggcaaa cattaaagac gattcgctcg
     9001 tgcagcataa ccagcgtggg ttcattacat ttgcgactgc cgggccgaac acacgaacca
     9061 cgcaagtctt catcaattat gggaacaacc tggctctcga taaaatgggc ttcacaccgt
     9121 tcggtcaagt tacggaggga atggaagttg tcgacaagct ctacagcggt tatggcgagg
     9181 gcgctcccga cgggaaggga ccggaccaga gtaaggttgg caaccaaggc cgcgtttacc
     9241 tgatgaagaa tttcccgttg ttggatagca tcaagacggc aacgattgtg ccaccagccg
     9301 cgcctggatc tgcgcctgcg gcggctcccg ctccggctaa gtaattgttt tcatcgggaa
     9361 tttcgcggaa ggaatttggt ttgcttacac tgagtcgact gaaataattt tctggcggtt
     9421 gctcagggca aaattaaatc gctagtggaa ataaaacggg cttcatcctg cggaattttt
     9481 gagcatgcgc tcatataaat ccgcaggcta aagcccgcgc tactgaaagg ctacgctacg
     9541 cccaggatcg tgactagttc gcgtaccgag gctgcggact tgtccagagc agctttttct
     9601 tccgcagtca gcttgatttc gattatctgt tcaatgccgc gcgcgccgag ttttacggga
     9661 acgccgacat agaggccgtt aattccgtac tcgccttcga gataagcggc gcagggaaga
     9721 atttttttct tgtccttcag tatcgcttcc accatttcga caacggctgc ggacggcgcg
     9781 taataagccg agccggtctt cagcaggttg acgatttccg ctccgccctt gcgcgtgcgg
     9841 tctacgatcg ccgcaatgcg gtcggcaggg agcaggtcgg gcaggggaat gccagcgacg
     9901 gtcgagtaac gcggcagggg caccatatca tcgccgtgtc cgcctaggac gaatgagttt
     9961 acgttttcta ctgacacacc aagctccatc gccacaaacg agctcatgcg cgcggagtcc
    10021 agaacgcccg ccatgcccat cacgcggttc ttggagaagc cgctgacttt gaatgcagcc
    10081 tgcaccatgg cgtccagagg atttgtcacc aggatcagaa tggcgttcgg tgaaaatttt
    10141 acgatttgct cgaccactgc tttaacgacg tcgtaattcg ccttcaaaag atcatcgcgg
    10201 ctcattccag gcttgcgcgg gaatcccgcg gtgactacca cgacatcgct gttcgctgtt
    10261 tccttatagt cgccggatgc cgttgaaact ccggtgacgc gcgcatcgga gccggtaatc
    10321 ggagtggatt ccaccatgtc tagcgcttta cccgcaggag gcccgtccag aatatcggtg
    10381 agcaccacat cagccagctc caagtcaaca atgcgctgcg ccgttgtcga tcccacaaat
    10441 ccgccgccaa caactgtaac tttcttccgc atgaatgcct tctcctatag tttttgattg
    10501 agaatgagag tgataagcag cggtagcctg ggccaccgct gctttgggat ttactttgtt
    10561 accgccatgg attgcatgtt ttcgatgatg gcgcttgcaa attccgcggt gccgagtttc
    10621 gttgcgcctt ccatcattcg ttcgaggtcg tacgtaactt tcttctgtcc aacggtgcgc
    10681 tcgatgcccg actcaatcag cttcccgact tctttccagc ccatgaaatc gaacatcatc
    10741 acgccggaga gcatgacgga gctgggattg atcacgttct tgtcagcgta cttgggagcg
    10801 gtgccgtgcg tggcttcgaa acagccgtag ccatcgccga tgttggcgcc cggtgccatg
    10861 ccaagtccac ccacttgcgc ggcgcaggca tcggaaatgt aatcgccgtt caggtttggc
    10921 gcggcgatta cttgatactc atcggcgcgc gtaatgacct gctggaagat ggaatcggcg
    10981 atacggtcat tgatcaggag tttttgtttc cacttgcctt ggccgtgcga agggtaaatt
    11041 ttttcgagca ccgatttcac ttcgtcgcac acggatttgc ggaacgcttc ggtgccttgt
    11101 tccatccccg gttcgataag ggcggcattc tgctcgatag taaggttcgc atccttgtcc
    11161 ttattgccga gaatccaggt ctcgcgttcg gtgacagtcg gcgctcggaa ttcttcgacg
    11221 gcgagttcgt agccccaatc acggaacgaa ccttcggtaa atttctgaat gttacccttg
    11281 tgcatgatgg tgacgacgcg cttgttgttt tccaaagcgt attcgatggc gcggcgcatc
    11341 aggcgtttgg tgccaaacgg ggagatgggc ttgatggcaa tggaggaatc cgcgcgaatt
    11401 tgcttcgggc cgccgccgag catgtccttg tttagaaatt caatgagctt tttcacttcc
    11461 ggcgtgccag atttccactc gataccgatg taaacatctt cggtattctc gcgataaatc
    11521 acgacgtcca tgcgctcggg atgttttacg ggagaggcga cgccctggaa ataacgcacg
    11581 gggcgcacac atgcgtagag atccagaatt tggcgcaacg ctacgtttag cgagcgaatg
    11641 ccgccgccca cgggagtcgt cagcggcccc ttgatcgaaa tgcggaaatc acgcacagct
    11701 tcaaccgttt cgtccggcaa ccatttttta aactcattga atgctttttc gccggcgaac
    11761 acttcgaacc aggaaatttt tcgtttgcca ccgtacacga tttgaacagc ggcgtcgaac
    11821 acgcgttgcg aagccttcca gatatcgcgt cccgtcccgt cgccctcgat atagggaata
    11881 ataggattgt cgggaatgac gaatttgccg ttgacatact gaattgcctg tccgtcagat
    11941 ggtatggccc tgccgttata cgatggcttc atgtctattt tcctctgtgg aatatcactg
    12001 cacgagcgcg aatgggagag aataacacac ccaatacccg caagacatat cgagcaaaat
    12061 caaaaaagtt gttcatacaa gcccaaagcc aatttggcac aattgattcc gggcaatacc
    12121 gtcattagtc gtaatattcc tggcccagat gcgtaattaa atcttcgccc atcatccagc
    12181 gcagcgtgtt tttcagcttc atcgattgaa tgaagagatc gtgttccgga tacagctctg
    12241 gcgcgcacat gggcgatttg aagtagaagc tgagccattc ctggatgccg cgcatcccgg
    12301 cgcgcttggc gaggtcaagg aataaaacga ggtcgagcac gatcggtgct gcgagaattg
    12361 agtcgcggca gaggaagtca actttaatct gcatctggta gcccagccat ccgaatatgt
    12421 cgatgttgtc ccagccttct ttgttatcgc cgcgcggcgg atagtaattg atgcggacct
    12481 tgtggtgaat ttgtccgtac aaggtcgggt acaacttcgg ctgaagaata tattcgagca
    12541 cgccgagctt ggattcttcc ttggtcttga acgattcggg atcgtcaaga acttcgccat
    12601 cgcgatttcc caaaatgttg gtcgagaacc aaccattcat tccgaggagg cgcgccttga
    12661 aacccggcgc gagaatcgtc ttcatcaatg tttgcccggt cttgaaatcc ttaccgcaaa
    12721 tgggcacgcc gtgctgcttc gagagcgtca ccatggcagg gacgtcgacc gtcaggttgg
    12781 gagcgccgtt ggcaaacggc acgccactcg taatcgcggc ccatgcataa agcatggaag
    12841 gcgcgatggc cggatgattt tccttcatcg cggcttcgaa tttttcagga gtttcgtaga
    12901 cggcatgcgg ttttagaaaa atttccgtcg agccgcacca gatcacgact gtgcgagaga
    12961 ctttattttt cttttgaaag ctttggatgt cggctttgat ctgcagggcc aaatcatact
    13021 tggtgcggcc ctttttcacg tgcgtgccgg tcaatttctt tacataattc tggtcgaacg
    13081 cggctttcat cggcttgatt ttgcttaaaa actttttgca ttcggccaga tgctcgttgc
    13141 tcaggaccgc cgcatttttg gcggcttcat acgcgctgtc cggaaaaata tcccagctgc
    13201 cgaaaacaat gtcctcaatg ctggcgatgg gcacaaaatc cttaattgcc ggggcacggc
    13261 cttcggtccg ctttccgagt cggatcgttc ccatttgcgt cagggaaccg atgggctgcg
    13321 atttctttcg ccggatcaaa tccactccgg ccataaatgt ggtggcgaca gcccccatgc
    13381 cgacggtgag gaccccaagt tttcccttcg gcaaggcaat gcccgcggac ttgcgcattc
    13441 tgatttctcc tgtcggtttc aatgtgtaca gacctgaagc agcgcgcggc ttttcttcat
    13501 ccgcgcacat taagcgaaat ttctttatcg caaaccaagt atcatgcgcg aatgtaagca
    13561 gatttgcaac gcggatctgc cccatctatc gagccattta tgttcttgaa attggggcga
    13621 tgaacataat ccaaatacca acgaacagtg caatcacctc aatgccgaga accgtccagt
    13681 aaataagagg ttcctttttg cgaggaagcc acacaaacca tcgcaggtcg agcaactttg
    13741 cagatgcgag ttggtataga accacacaaa acaagaccga cagtgctatc ccttgctgat
    13801 cccgtccggt cattgctacc aaagcatcaa gagaagtcag cataggtttt gtcagcacat
    13861 tgcaccaacg caactttggt acactgctaa taaaataata gagaccgaag tcagtttaca
    13921 ttgcgcgcct tggccgacag ttttttcgaa gctgccgatt tttgaaccgt tttttgtggc
    13981 gctgaaccgg gcttggctct tagtcctgag agcatctcgt cgagtcccag agtgttgatc
    14041 acatctttct tttcgatcca gcctcggcgc gccatttgca cgccgtagcg catcgtctgc
    14101 aatccatggg tggaatgcgc atcggtggag atcacgatct tcacgccgcg ctctttggcc
    14161 attcgcaggt gaatatcttt taagtcgagg cgctcgggag acgcattgca ctccatcact
    14221 acattatttt ttttcgcagc gtccagaatg cgctccatat cgtacgcata cgcgtcgcgt
    14281 cgaagaacca ggcggccggt tggatgtccc acaatttgcg tgtagggatt ttcgatggcc
    14341 gccaaaattc tgtccgtcat ttcggcgcgt tccatggtca tatacgaatg gatggacacc
    14401 agcacaacat ccagctgggc cagaacttca tcgtccatat ccagtttgcc atttttcaag
    14461 acgtcgactt cgctgctcga gaggactcga attcccaagt ccttggaatt tgcggcttga
    14521 attttcttaa tttgttcgag tgtgcgtttt tcatcgagcc cgttggcgac ggttacggct
    14581 ttgctgtgat ctgtcagcga aatatatttg tagccgagat cttttgcggc aagcgccatc
    14641 tcttcgatcg agttttttcc gtcgctcgcc gtggtatgca tctgcatgtc gccgagaatg
    14701 tcgcgaagtt cgatcaggtg ggggaggtgg tgctcggcgg ctgcatcgat ttcgcctgta
    14761 ttttcgcgaa gttcgggcgg cacgtagtcc agcttcagct tggcatagat ttctttttcc
    14821 gttgcgcgtg ccaccggatg ttcgccctcg agcgttgcca gcgcatactc gttcaatgtc
    14881 aatcccattt tattggcacg tcctcgcaag acgacgttgt gttccttcga accagtgaaa
    14941 tacagcaggg ctgcgccgta actttcctta tcgaggattc gcacatcgac ttgcagggca
    15001 tttcttagaa gaaagctgac tttattttcg ccatgcgcca gtgtctggtc gattcccggg
    15061 aatgccaaaa tgtgctgtga aatggattcc accgtttcca ccgtgctttt tccgtgtgcg
    15121 agcgtcacaa gcagatcaag atctccaatc gtttccttac atctgcgcag cgagcctgcg
    15181 ggagtgactt cctcaatagg tttgccgaag gcgcgaatgt gagaaactaa aatgttggcg
    15241 gtctgctccg cttcgtcgat gtggaatcgc cccgcggatt ttttgtaaac ggaaattgcc
    15301 tttaaaatat tttcttcagt tttttctcca aagccgggca gatcgcgcaa cttcccctcg
    15361 cgcgcaagtt tttccacctc atctactgtt ccggccttga acgctttcca caggaaggcg
    15421 acttttttcg gcccgaggga ttgcaattcg agaagattga gaatcgttgg cgggtatttc
    15481 ttaagcagtt tggcg
//
LOCUS       DASZMB010000104        19881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2299988, whole genome shotgun sequence.
ACCESSION   DASZMB010000104 DASZMB010000000
VERSION     DASZMB010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 19881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19881
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2299988"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VG272_04715"
     CDS             complement(<1..624)
                     /locus_tag="VG272_04715"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HWF13017.1"
                     /translation="MNENALATLRGNTHPLAQAQYDQGIAPPDLPMARMLLVLKRSDA
                     QESTLQALLDAQQDPNSPGYHQWLTPDAFGQQFGPSDQGIQAVATWLTSHGFQVGGIS
                     RGRTVIEFSGTAAQVQQAFHTEIHKYVVNGEQHWANASDPQIPAALAPVITGINSLHN
                     FSKKPVHRVGGVYSKTRATGEVKSIRPAFTYQDFSQCEQVGLCYFVGP"
     gene            1084..2010
                     /locus_tag="VG272_04720"
     CDS             1084..2010
                     /locus_tag="VG272_04720"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HWF13018.1"
                     /translation="MASNVKRDKTFSQSVNWVTTFFMVAFHIGAIAAFFFFSWKPVLV
                     AVLLWWVSGSLGIGMGYHRLLTHRGYKTPKWVEYFLTTCATLALEGGPIFWVATHRKH
                     HQNTDKEGDPHTPREGGFWAHMGWILTGKAMHNKLDSLLPYVPDLRKDKFHVWISEWH
                     WITQVVVGVALLVFGGLPYLLWGSFVRTVFGLHSTWLVNSATHMWGSQRFITGDDSTN
                     SFWVAVLTFGEGWHNNHHAHPQMARHGMTWYEIDMNWYGISALKAMGLAWDISLPKLG
                     REKSIKPLAVPETPAILAPVVSDVVPQEAEGD"
     gene            complement(2091..2585)
                     /locus_tag="VG272_04725"
     CDS             complement(2091..2585)
                     /locus_tag="VG272_04725"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWF13019.1"
                     /translation="MKFIWVLIISMMASFALPAKTHAQNKRFESATPTALEKQLYDVE
                     LKWMKAEHDKLMDGPNSMNELWMDSFFDVLSSGVVVDKHEMMDMMNKADSRPGTGAFP
                     DTFKVRAIYGNVVLATDHTTIKGLDANGNLVTVREMRVLRMFAKDKGKWRVAGAGLVV
                     IPPK"
     gene            2867..3772
                     /locus_tag="VG272_04730"
     CDS             2867..3772
                     /locus_tag="VG272_04730"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWF13020.1"
                     /translation="MRITGRAKKIAFFVTLGACLVGLAIALNVTWVVLNWRAVVPLVL
                     GIIFFGLIIAGLVVYTVFLVREIHKNEQQDSFLNAVTHELKTPIASIRLYLETLQTRQ
                     LSEAQHQDFYRIMLEDTDRLLGTVEQILKAGEARHASSKKNWEQVDFSGIVAETVELT
                     RLRHHFAPDVLRFAGPAAEKIYLRGNPQELRTAVFNLVENAVKYSGEIKEIEVDLLSP
                     DMDTILLRVRDKGVGIPHAELKRIFKRFYRVTNSATGRVKGTGLGLFIVRSIARRHGG
                     DAFAESEGTGRGSTFTLRLPRAYPV"
     gene            3769..4521
                     /locus_tag="VG272_04735"
     CDS             3769..4521
                     /locus_tag="VG272_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF13021.1"
                     /translation="MSRILIVEDEAHLAEGLRFNLEQEGYSVQVVGTGEEAIDRLLKE
                     KEEFDALVLDVMLPGKDGFTVARALREARNYIPLLMLTARARPEDVLQGFESGADDYL
                     PKPFILSIFIARVRSLLRRKTWEHGMPKPSAKPAMRTTESPDMFKFGDKVADFENLTL
                     EIGHEKVQLTLMEAELLRYLIENAEHPVSRKAILENVWNLQEDTDTRAIDNFIVRLRK
                     YIEKEPSDPRHLVTVRGLGYKFIPFPDKKSKK"
     gene            complement(4538..6640)
                     /locus_tag="VG272_04740"
     CDS             complement(4538..6640)
                     /locus_tag="VG272_04740"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HWF13022.1"
                     /translation="MTTNFGQYNLQSTFWRTLGCAAILILLVLACIEVLRAGQQQISA
                     KPSGASVPGVDLTILDKTCKPCQDFYHYASGEWLTKNPVPPAYPSWGRFSELAERNRE
                     LLHQILEEAAATKPPAGSNKQKIGDFYASCIDEKQVNAEGAKPLESEIARINSVQSLP
                     ELQGEIARLQGMGIRALFQFGSTQDEKNSSEVIGGADQGGLGLPDRDYYTKTDEKSQQ
                     LRQQYEQHVGKMLALAGDDENKAASEAKAIVAIETKLAEASMTRVDRRDPEKTYHRMN
                     RAQLKTLTPNWSWEDYFQEVGHTNINDVDISEPKFFEAMSQELKETPLDTWKTYFRWH
                     LLNSTAAYLSEPFVDENFNFNGRVLAGTKELLPRWKRCVSATDRQLGEALGQIYTEKY
                     FPPEAKARALDMVNHLIDALHDDLQTLPWMSPATRKEALTKLSAFTRKIGYPDKWRDY
                     STYAVDRGPYVLNQMRGSQFEFQRDLNKIGKPVDRGEWDMSPPTVNAYYNAQMNEIVF
                     PAGIMQPPFFDPKADDASNYGAIGAVIGHEMTHGFDDQGRKFDAEGNLRDWWTPEDEK
                     NFTRRAACVEKQFGDFVVQDDVHENGKLVLGESIADLGGLNLAYRALQKAEKGKKPTR
                     IGGMTPDQRFFIAYAQIWASNDRPEYERLLVNTDPHPLARFRAVAAPSNMPEFAHAFD
                     CKPGDAMARPSGIQCQIW"
     gene            complement(6768..7196)
                     /locus_tag="VG272_04745"
     CDS             complement(6768..7196)
                     /locus_tag="VG272_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWF13023.1"
                     /translation="MMGRRVAVLSALFCCVFVGCGGGKPAAQTKIPQEEIDRKNPVEA
                     NVASIEEGKRLYGATDCALCHGKDGDGKGLEAKDGNMNVHDWRKAQNLDHFTDGELSY
                     VILKGKGRMPSYNGRENPEQVWQIVNYIRSIPAGGSAPKS"
     gene            7628..8119
                     /locus_tag="VG272_04750"
     CDS             7628..8119
                     /locus_tag="VG272_04750"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HWF13024.1"
                     /translation="MHFLFSFCQWLEQTGIGTSIRESTWLFPVIETVHIFGIVMLVGA
                     TSVLDLRLLGWTYREKSVSKMAWRFLPWAWVGFSIQLTTGVLLFASEATKMYDNIGFQ
                     IKMVLIVVAGLNALLFHSIAYQSVDKWEYDAVGPLSARAAGLISIVLWFGIVAAGRWI
                     AYV"
     gene            complement(8190..9488)
                     /locus_tag="VG272_04755"
     CDS             complement(8190..9488)
                     /locus_tag="VG272_04755"
                     /inference="COORDINATES: protein motif:HMM:NF011656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF13025.1"
                     /translation="MSTQTTGSKIGNVMRVSSGNFLEMYDFMVYAYYASYIAREIFPS
                     SNAYASMMMTLGTFGAGYLMRPLGAVVLGAYVDRHGRRTGLILTLFLMAIGTLVIACT
                     PAYRTIGIIAPIVVLLGRLLQGFSAGVEVGGACIYLSEMATPGHRGFYCAWQSASQQV
                     AVVFAALLGVGLSAIMPPEQMSAWGWRVPFFIGCMIVPLLFWLRRSLAETEAFLAQKH
                     HPDIEEILASLATNWKIVVLGVLFSTMTTVCFYLITAYTPTYGIEVLHLTSRQSLLVT
                     FCVGMSNFFWLPAGGALSDIVGRRPILIGITCTAILTAYPAMLWLVSSPSTARLLIVE
                     LWLSIFFGVYNGAMIPHLAEIMPPEIRTSGFSLAFSLATAIFGGFTPAICTYLIHETG
                     NRAMPALWLSFAALCGLAAALVGGWQRAPVAELKSEVSAQ"
     gene            complement(9559..11157)
                     /locus_tag="VG272_04760"
     CDS             complement(9559..11157)
                     /locus_tag="VG272_04760"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HWF13026.1"
                     /translation="MRSQFKFVAVFGLALYLASSCYGATVSGTVKGQDGAPIEGAFVQ
                     TQNTKTKISFYVLSDHQGHYRVDKLPAGDYRMQAKATGFRAEPKSGVALTADQNFAAD
                     FSMQPGIVRWNELNIYQAKKLWPEESAKTTIFTKCFICHGFQTRMASVTRDADGWKDR
                     VQFMRETMHFSLSYRITDQDADEVATYLTKLYGPDSVLPKSAADAPGYKETLRPFTSE
                     AMKITYVEYDMPGPSRMPFSAAPAKDGSVWIPNFGIGNKITRLNPKTGEMQDFPVPNE
                     GTAAVHSAIAAPDGSVWLTEQASDKLGRWDPVTQKITEYQDAYRPGKEGREDGGSRHT
                     VRIDSKGMVWSSGYPLTRFDPETKKYYDFPEADHTYSLAFDKDENVWFTNPGTGEIGE
                     VNAKTFKVKQWMPPTHDSYERRLALDTDGTVWFGEFQKGKIGHFDPKTETFKEYDVPG
                     GIDSFPYAIAVAADHSVWYSSYYLDVVGRLDPKSGKIVEYPFPHSENTIREFFPDSEG
                     RMWYGSPSNNKVGYFYLTGDSAGN"
     gene            11768..16759
                     /locus_tag="VG272_04765"
     CDS             11768..16759
                     /locus_tag="VG272_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HWF13027.1"
                     /translation="MRVKPRFPGIRVTANGNQLVSYHTETRITDAGIFYPITPSTEGG
                     ELYQQAYAEGKLNVFGRSTIAVEAEGEHAAQGGAIAHSVCGQRVVNFTSGQGIVYGVE
                     QYYHAPGKCSTMVLEVGARALTKHALNVHCGHDDVYGSLDTGWITLFGKDAQQAADQA
                     LILRRVTELSLTPGMNVQDGFLTSHLERTFYKHESDLMREYLGAPDDMIECPTESQRV
                     LFGPTRRRVPRMIDLTNPVLLGPVQNQEHYMQGVVARRNNFTEPILQFLEDAYKVFGD
                     LTGRYYGLVTQYKTEDADTVFVSLGSAAENIEAGVDYLREHRNAKVGSIHLNVIRPFP
                     EAAVITAIKGKRNVIVLERTDEPMAGDNPMGRDIRTALSKALVTHDHAAEANLPAITP
                     ETMPRLFNGIYGLGSRDFRPEHILGAYEFATGTRARKDGKGIADGISYFVLGVDHPYE
                     VKSDETPSLLPDKSVAVRFHSIGGWGAITTGKNLGAIIGDLNDLLYDRDKVVDEFGNP
                     KEIIHVSANPKYGSEKKGAPTSYFMVAAPQRIRVNCDLRHVTVVLCCDPKAFTHTNPL
                     DGILEGGCLIWESEEEGEKAWERLPMWARKQIIDKNIRVYTLPGFEIARKATDRADLQ
                     LRMQGNAFLGAFFSVSPILREFGITGEQFREVVHKQYVKKFGRLGDAVVNSNMEVMTQ
                     GFERVREIRVGEMTAPDHSSLRGEALLPMLAAHTDGGGCGSGCRSHEVPAGQDPRTPL
                     STIAKFDAEFRSNYGYNQPASPLASLGVMAAGSGDTASKYVARRETPLYIPENCTQCM
                     ECIAVCPDTALPNCSQDLETVLRTAIGNYVADPNERSKMLRLVPEIDKRTHALMRDSL
                     AAKALTPLPEILRKVTNEVDGFSDQAKCEFFAVIEKVPMAFQKVNAIFSTPERKTPGA
                     GGIFSIFVSDLCKGCAACVTACGDHNALRMVQETEQVNAEHETGTAFLNLLPDTSQKY
                     LGLYNDTHPDESKTAALRNLLMVRRNYDALVAGDGACAGCGEKSILKAITSVTEAYMR
                     PIYHAKANRLRTKAGDLEQAGTQKLAELKARNPKEYELLRQAIAHLVMGLGGEDEKDT
                     RKRIAAHGEITDSDLVHSIATVMLQEAFNHMNLQAIDGRLANGMSVMAMAAHTGCNTV
                     YGSTPSNNPHPYPWMNSLFQDGVTVGWLLGESFIVDHAHRSVIPERLADVLMQRAENV
                     INDREYYEYSHFTDALMTDQEIQELPKVWVVGGDGGMGDIGYQNTSKVILQNRPNVKA
                     LMLDTQVYSNTGGQNSDSTPMLGGNDMNVFGAATQGKNVEKKTVAETFLAGHGSPFLA
                     QISIANAPKLYRAILDGLEYRGTAFLQCFTTCQPEHGVADDMALTQAQRVRDSRGAPE
                     FIFNPRLGETYNEALDLKGNPSLEKDWYETKFKTTNEPYRYTVAHWCATEARFRNHLK
                     KIKKEDAAKLIPLENMLVRITQQDVVYRRYLHPENRSFIPDFGVYIKVQGSNGEIEYR
                     SLSRQLVLFCVERRKSWRMMQSKSGIENKEYKAQRSILADVDAGKISCEDFFARAEEL
                     MKERTPSAPEHAPRPAAPAVAQATAPLKAAATAPAVAAKAQAKVWEVVTKSVAIHPPK
                     PVTVASKPFQAAEKKELEDDSAEWEERVQIRLMQLDVDPGNDADQGVITKSGD"
     gene            complement(16874..17323)
                     /locus_tag="VG272_04770"
     CDS             complement(16874..17323)
                     /locus_tag="VG272_04770"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWF13028.1"
                     /translation="MNLIDSIGLLLKQKGQNVWHISPEACVYDAIEMMAEKHVGALLV
                     VSDGELVGVVSERDYARKVILQGKSSKETQVKEVMTSPAVFVTPEQTVEDAMRIMTEK
                     HIRHLPVVGNGAILGVVSIGDMVKWIISAHEHTILQLNSYISGTYPG"
     gene            17553..19364
                     /locus_tag="VG272_04775"
     CDS             17553..19364
                     /locus_tag="VG272_04775"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWF13029.1"
                     /translation="MKSFRWIFAVLIVLLWSSLNSNAQTTKGAVDYEEHCASCHGNPK
                     SATPAPDIMSLWKLTAEDIYAAREKGAHSTLQGATDDNWREIAFYLGRRRVDIAKVAD
                     ARLMPNQCPGNSPIGDLSAKPLWNGWGNGIANTRFQSAKEAGLTAAQVPNLKLKWAFG
                     FPGADEVYGQPTVAGGRVFLGVDTGAVYSIDAETGCVYWSYQADAGVRTAITISPLKG
                     KGAAKYGVYFGDIRANVYMVDAATGKEIWKVKVEDHPVAKVTGAPTLYEDRLYVPVSS
                     QEERSAGWSTVYPCCTFRGSVVALDASNGHTIWKTYVVQDPPKPTGKTPSGVQQYGPN
                     GGAVWNSPTIDPKNHALYIGTGDSYTKPVSKNTDAIVALDLKTGKIMWSIQDTENDAW
                     LSGCGGEIKSENCLPPGEIGPDYDFGASPILVSLGNGHRMLVAGQKSGMVWGHDPDRE
                     GTVVWKAQLVKKLALGMITFGGASDEQNSYFGLRTGGVAAVRNDTGEQKWFTPVPPPP
                     GPGPKGENAAITAIPGAIFSGGWDGMLRAFSTEDGHLLWDYNMVRSYETVNHVAAKGG
                     SMAAAGPTVAGGMLFVGSGYVFGGGVPGNALLVFAAQ"
     gene            19557..>19881
                     /locus_tag="VG272_04780"
     CDS             19557..>19881
                     /locus_tag="VG272_04780"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HWF13030.1"
                     /translation="MQHFGAFVIGIAIGFLGGLFGKGGSAVATPLLSLIGFPGFIAVA
                     SPLPATIPGTLIASAEYWQSKLIDWQIVLWSIGVGIPATTFGSMITEFTGARILLILT
                     AILVLG"
BASE COUNT         4573 a         5493 c         5299 g         4516 t
ORIGIN      
        1 agggccgaca aaatagcaaa ggccaacttg ctcacactga ctgaaatcct gatacgtgaa
       61 agctgggcga atagatttca cttctcctgt ggcgcgcgtt tttgaataaa cgccgccgac
      121 gcggtgcacg ggcttttttg aaaaattgtg cagagaattt attcccgtaa tcaccggggc
      181 gagggcagcg ggaatttgcg gatcgctggc gttggcccaa tgctgctcgc catttacaac
      241 gtatttgtgg atttcagtat gaaatgcctg ctgcacttgc gcagccgtgc cggaaaattc
      301 gatgaccgtc cggccacggg aaattcctcc gacttggaat ccgtgcgaag tgagccatgt
      361 agcgactgct tgaatgccct gatcgctggg cccgaactgc tgtccgaacg catctggcgt
      421 aagccattgg tgatagcccg gagagttcgg gtcctgttgg gcgtcgagaa gcgcctgcag
      481 cgtggattcc tgcgcgtcgc ttcgcttcaa aacgagcaac atgcgcgcca tgggaagatc
      541 gggtggagcg atgccttgat catattgagc ttgcgcgagc gggtgcgtat tcccgcgaag
      601 cgtcgcgaga gcgttttcgt tcacgcgttc gaggatgcgt gactgagtag ggggtgtttg
      661 tgaccaggcg gccactgaaa gaaacgttga gcaaaagtaa acgaaaagaa acctgggatg
      721 ggacaggttg cttacacgca tcgcgccgcc tttggcaaat tcgagtggcg atattgttat
      781 cataaaggca acaaagttca atgcttatta tttaagtacc cacgtttatt tgcctttgag
      841 aagagcgtgc tggtacgtct ataacttggc cggctgaggt ctatctagga atccctttgc
      901 tttgcatctt ggcttcaggg aaagcattcc ccattcacaa tgtgccaacc tgggatggtg
      961 tcacatccgc ctcatttgcc tacaattaca gaactattac aaagcgtttt cgcgccccgt
     1021 gctactttcg atttaggcgg cgataggcgg aacaagaggg ccgcatgtaa aggggaccgg
     1081 gacatggcaa gcaacgtaaa acgggacaag acattttcgc agtcggtgaa ctgggttacg
     1141 acctttttca tggttgcatt tcatatcggt gcgattgccg catttttctt ctttagttgg
     1201 aagcctgtac ttgtagcggt gctgttgtgg tgggtttcgg gaagcttggg cattggcatg
     1261 ggctatcacc gtttgctgac gcatcgcggc tacaaaacac cgaagtgggt tgaatatttc
     1321 ctgaccacct gcgcgacact cgctctggaa ggtggaccga ttttctgggt ggccacgcac
     1381 cgcaagcatc atcaaaatac ggacaaagaa ggcgatccgc atacgccccg cgaaggtgga
     1441 ttctgggctc acatgggatg gatcctcacc ggcaaggcca tgcacaataa actcgattcc
     1501 ctgctgccct atgttcccga ccttcgcaaa gacaaatttc atgtttggat cagcgagtgg
     1561 cactggatta cgcaagtggt agtaggcgtt gcgctgcttg tgttcggcgg gctgccttat
     1621 ctactttggg gctcatttgt acgcaccgtg ttcgggcttc actccacgtg gctcgtgaat
     1681 tccgcgacgc acatgtgggg ctcgcagcgc ttcattaccg gtgacgattc cacgaacagt
     1741 ttctgggtag ccgtcctgac gttcggagaa ggttggcaca acaatcacca cgcgcatccg
     1801 caaatggcgc gccatggaat gacatggtat gaaatcgaca tgaactggta cgggatcagc
     1861 gcgcttaagg cgatgggcct ggcttgggac atttcgctgc cgaaactagg ccgcgaaaaa
     1921 tcgatcaagc cgctggcggt tcccgaaact cccgcgatac tcgcaccggt cgtctcggac
     1981 gtagttcctc aggaagcgga aggcgattaa gccggccaaa ttgctaaggc taactttcaa
     2041 ttatttttga ttcggggcac gcatccaaaa aagcgtgccc cgtttttttg ctacttggga
     2101 ggaatgacga ccagtcccgc gccggccact ctccatttcc ctttgtcttt cgcaaacatt
     2161 ctgagcacgc gcatctctct caccgtaacg agatttccgt tcgcatccag acctttaatc
     2221 gtcgtgtgat ccgtcgcaag cacgacattt ccgtaaatgg cgcggacttt aaacgtgtcg
     2281 gggaatgcgc ccgtaccggg ccgcgagtcg gccttattca tcatgtccat catttcgtgc
     2341 ttatcgacca ccacacccga cgaaagcacg tcgaagaacg agtccatcca caactcattc
     2401 atgctgttgg ggccgtccat caacttgtcg tgctcagcct tcatccattt cagttccacg
     2461 tcgtaaagct gtttttcgag cgcagtcggg gtcgcgctct caaatctttt attctgagcg
     2521 tgtgtctttg caggaagagc gaaactagcc atcatggaaa tgatcaacac ccaaataaac
     2581 ttcatgtcga attcctcctt tgaggtttgc agttatttca tgacattaaa ttaaacccgc
     2641 ttactccccc acgaagaact ccgtggcgga gtggccgcta cgaaaaccga gcctgaagtg
     2701 ccttgagttt cgtagcggac gtccgaattt gggacgggcg cggcatgttc tggcgcccca
     2761 tatctgaccc acccccataa cttaattcct ggagtgttca aatctctcac accctgcgtt
     2821 ttttttatct cgcatctcga ttactatgtt cagatagaaa cctgtgatgc gcattactgg
     2881 acgtgccaag aagattgcgt tcttcgtaac gctgggcgcc tgcctggttg ggctggcgat
     2941 cgcgctgaac gttacgtggg ttgtcctgaa ctggcgcgcc gtcgttccgc tggtcctcgg
     3001 aattatattt ttcggactaa tcatcgccgg acttgtggtt tacacggttt ttctggtgcg
     3061 cgaaattcac aagaacgagc agcaggatag tttcctgaac gcggtcaccc acgaactgaa
     3121 aactcccatc gcctccattc gcctgtatct cgaaacgctg caaacacggc aattgagcga
     3181 agcgcagcat caggatttct accgcatcat gctcgaagat acggacaggc tattgggaac
     3241 ggtcgagcaa attttgaaag cgggcgaggc gcggcacgcg agcagcaaaa agaattggga
     3301 gcaagtggat ttttccggca tcgtggcgga aaccgtcgaa cttacccggc tacggcatca
     3361 ttttgcgcct gatgttttgc ggttcgcagg gcctgcggca gagaaaattt atttgcgcgg
     3421 gaatccgcag gagttgcgta ccgccgtatt taacctcgtc gaaaatgcgg tgaaatattc
     3481 aggcgaaata aaagaaatcg aagtcgacct gttaagcccc gacatggata cgatcctcct
     3541 ccgtgtccgc gacaaagggg tgggaatccc tcacgcggaa ttgaaacgta tttttaaacg
     3601 cttttaccgc gtgacgaatt ccgcaacggg ccgcgtgaaa ggcacgggcc tcggcctgtt
     3661 cattgtgcgc tccatcgcgc ggcggcatgg aggcgatgcg ttcgccgaga gcgaaggcac
     3721 aggacgcggg agcactttca cgctgcgcct gccgagggcc tatcccgtat gagccgcatt
     3781 ttgattgtgg aggatgaagc gcacctggcc gaaggactgc gcttcaatct cgagcaggaa
     3841 ggctactcgg tccaggtagt gggcacgggc gaagaggcaa tcgaccgcct cttgaaagaa
     3901 aaagaagagt tcgacgccct ggtgctggac gtcatgcttc cagggaaaga cggttttacg
     3961 gttgctcgag cgttgcgcga agcccgcaat tacattccgc ttttaatgct caccgcgcgc
     4021 gcgcgtcccg aagatgtgtt gcagggattt gaatccggcg ctgatgatta tttaccgaag
     4081 ccattcattc tttcgatttt catcgcgcga gtccgcagcc tcttgcgacg caaaacctgg
     4141 gagcacggaa tgccaaagcc ttccgccaaa cccgccatgc gcactacaga gtcgcccgac
     4201 atgtttaagt tcggagacaa ggttgcggat tttgaaaatc tgaccctgga aattggccat
     4261 gaaaaagtgc aactgacgct gatggaagcg gaattattgc gttacctgat cgaaaacgcg
     4321 gaacatccgg tttcgcgaaa agcgatcctg gaaaatgtgt ggaacctgca agaggatacg
     4381 gacacgcgcg ccatcgacaa ctttatcgtg cgcctgcgaa aatatatcga gaaagaaccg
     4441 tcggatccgc ggcaccttgt aacggtgcgc ggcctcggct acaaatttat tccgttccct
     4501 gacaagaaat cgaaaaaata atcccgcgag aaaccaatca ccaaatttgg cactgaattc
     4561 cggatggccg ggccatcgcg tcaccgggct tgcaatcgaa tgcgtgcgcg aattccggca
     4621 tgttggatgg agccgcaacg gcgcggaaac gcgcgagtgg gtgaggatcc gtgtttacga
     4681 gcaagcgctc gtattcggga cggtcattgg acgcccagat ttgtgcgtag gcgataaaga
     4741 agcgttggtc gggtgtcatg ccgccaatcc gcgtgggctt cttccccttc tccgctttct
     4801 gcagagcgcg gtaggcgagg tttagcccgc caagatcggc gatgctttcg cccaacacga
     4861 gtttgccgtt ttcgtgcacg tcatcctgca ccacgaaatc gccgaactgt ttttcgacgc
     4921 acgcggcgcg ccgggtaaaa ttcttttcgt cctcaggagt ccaccaatcg cgcaagttgc
     4981 cttccgcgtc aaacttccgg ccttgatcgt cgaagccgtg cgtcatttcg tggccgatta
     5041 cggctccaat cgcgccatag ttggaagcgt catcggcctt cggatcgaaa aacggaggct
     5101 gcataattcc cgccggaaaa acgatttcgt tcatttgcgc attgtaataa gcattcacgg
     5161 tcgggggaga catgtcccac tcgccgcggt ccacgggctt gccgattttg ttcagatcgc
     5221 gctggaattc aaattgagag ccgcgcattt gattgaggac gtacggccca cgatcgacgg
     5281 cataggtcga gtagtcgcgc cacttgtccg gatagccgat tttgcgggta aaggccgata
     5341 gcttcgtaag cgcctccttg cgcgtggcgg gactcatcca cggcaatgtt tgcaaatcgt
     5401 cgtgcagcgc gtcgatcagg tgattcacca tgtccagcgc gcgcgccttg gcctccggag
     5461 gaaaatattt ctccgtataa atctggccaa gtgcttcgcc caactgccgg tccgtggcgg
     5521 agacgcagcg cttccaccgc ggcagcaatt cttttgtccc cgccaagact cgcccattaa
     5581 aattgaaatt ttcatccacg aacggttcgg acaaatacgc ggctgttgaa ttcaacaagt
     5641 gccagcgaaa atatgttttc caggtgtcga gcggagtttc tttgagttcc tggctcatgg
     5701 cctcgaaaaa tttcggctcg gaaatatcga catcattgat attcgtgtgg ccaacttcct
     5761 gaaaataatc ttcccaggac caattgggag tcagtgtttt cagctgcgcg cgattcattc
     5821 gatgatacgt cttttccggg tcgcgccgat cgacgcgggt catggaagcc tcagctagct
     5881 ttgtctcgat tgcaacgatg gccttggcct cggaggctgc cttattttca tcgtctcccg
     5941 cgagcgctag cattttgcca acgtgctgct cgtattgctg gcgcagttgc tgcgatttct
     6001 catccgtctt tgtgtagtaa tcgcgatcgg gcaatccgag tccgccctga tccgcgccgc
     6061 caatcacctc ggaactgttt ttctcatcct gcgtggaacc gaattgaaaa agcgcacgga
     6121 ttcccattcc ctgcagccgc gcgatttccc cctgtaattc cggcaggctc tgaacgctat
     6181 taattcgagc gatttccgat tcgagcggct tcgcgccctc cgcattgacc tgcttttcgt
     6241 ctatgcagct tgcataaaaa tctccaattt tttgcttatt cgatccggcg ggcggctttg
     6301 tcgccgcagc ttcctccaga atctgatgaa gcagttcgcg atttctttcg gcaagttcgc
     6361 tgaagcggcc ccagctggga taagcgggtg gcactggatt tttcgtcagc cattcaccgc
     6421 tggcgtagtg atagaaatcc tggcagggtt tgcaggtctt atccagaatc gtaagatcca
     6481 ctccggggac cgatgctccg gacggttttg cactgatctg ctgttgccca gcccggagca
     6541 cttcaatgca agcaaggacc agtagaatta aaatagcggc acatccaagc gtgcgccaga
     6601 aagtactctg taaattatat tgaccaaaat tcgttgtcat tcttgcagta aagcgtttta
     6661 ttgaaattga cacagttatt acctcgatga accttccgcg atcattctca tccggacagt
     6721 gacacgccgt tcagaacgcg taccccactc aagtttgatt ctttgcccta ggatttcgga
     6781 gcgctcccgc ccgcgggaat tgaacgaatg taatttacga tctgccaaac ttgctcggga
     6841 ttttcgcggc cattgtagga aggcatgcgg cccttgccct tcagaatcac gtaggaaagc
     6901 tcgccatcgg tgaaatggtc cagattttga gcttttctcc aatcgtggac attcatgtta
     6961 ccgtctttgg cctccaaacc tttcccgtcc ccatctttcc catggcatag cgcgcaatcc
     7021 gtcgcgccat acaagcgctt gccctcttcg atcgaggcaa cgttggcttc cactggattt
     7081 ttgcggtcaa tttcttcctg gggaatttta gtttgtgcag caggtttacc gcccccacac
     7141 ccaacgaata cgcagcaaaa aagcgcggat agaaccgcaa ctcgccttcc catcattttc
     7201 cccctcgcac tcggaatatc gaacggaata attccaccca attgattatg ctgtttatca
     7261 aaacgaagtt tatatttttt ccgctggccc gtacagcgaa accgagatat cgctgcttcc
     7321 tgttttccgc taaactgttg gtcgacccgt tgatttcatg ttcgcaacct gactgggaga
     7381 tgatttggca tggcaaccac gtccttgtgc tgcgcgtcga tctgctgtaa aagatcagac
     7441 tcgacgacgg ccatggaagc tcataaagca acttggtgcg aaacggtgca aggcaattca
     7501 gcgtctaatt caaagaacct ttcgacggca gatgcgcaaa atgattgagc cgccgaaaat
     7561 agattttgtg ttttgaccgt ggctttagca gaattaactt atccgatgga tcgtgagggc
     7621 cagggagatg catttccttt tttcgttttg ccaatggctg gaacaaacag gaatcggaac
     7681 atcgattcgt gaatccacgt ggctttttcc cgtcattgag acagttcata ttttcggaat
     7741 cgtgatgctc gtgggcgcga cttcggtttt ggacttgcgc ctgctgggat ggacctaccg
     7801 cgaaaagtca gtgtccaaga tggcatggcg gtttctacct tgggcctggg tgggtttctc
     7861 cattcagtta actaccggcg ttttgctgtt cgcctctgaa gccaccaaga tgtatgacaa
     7921 tattggcttc caaatcaaga tggtgctcat cgtcgtcgct ggattgaacg cccttctatt
     7981 ccactctatc gcctaccaaa gcgttgataa gtgggaatat gacgccgtag gaccgctgag
     8041 cgctcgcgcc gcaggcttga tttccatcgt attgtggttc ggaattgttg cggcaggccg
     8101 ctggatcgcc tacgtctaaa aacgattgat gtctttaagt ggcgcgggct tcagtgccac
     8161 ttatattaat tgacaatttt ttcgaccgtc tactgcgcgg aaacttcgga cttcaactcc
     8221 gcgaccggcg ctctctgcca tccgccaaca agcgccgcag caagcccaca cagcgcagca
     8281 aacgacagcc acagcgccgg catcgcgcgg ttcccggtct catggatcag gtaggtgcaa
     8341 atcgcgggcg taaatccgcc aaaaatcgcc gtcgccaaac taaacgccag cgagaagccg
     8401 gacgtgcgaa tttccggcgg cataatttcc gcgagatgcg gaatcattgc gccgttgtac
     8461 acgccgaaga aaatggacaa ccacaactct acaatcaaca agcgcgcggt cgatggactg
     8521 ctgaccagcc acagcatcgc gggatatgcc gtcaggatcg cggtgcaggt gatgccgatc
     8581 aggatgggcc gccttccaac aatgtccgag agtgcgccgc ccgcgggaag ccagaaaaag
     8641 ttggacatcc ccacgcagaa tgtaacgagc aggctttgcc ggctggtcag gtgcaacact
     8701 tcaatcccat acgtcggcgt gtacgcggtg atcaggtaga aacacaccgt cgtcatcgtg
     8761 gaaaacaaaa ctccgagcac aacgattttc caatttgtcg caagtgacgc gagaatttcc
     8821 tcaatatccg gatgatgttt ttgggcaaga aacgcttccg tctccgcgag cgacctgcgc
     8881 agccagaaca acagcggcac aatcatgcag ccgatgaaaa atgggacgcg ccatccccat
     8941 gcggacattt gttccggcgg cataatggca ctgagcccca ctcccaacag cgcggcaaaa
     9001 accacggcga cttgttggct agccgactgc cacgcacaat aaaatccccg gtggccgggc
     9061 gtggccatct cagacaagta aatacatgcg ccgcccacct cgacgccggc cgaaaaacct
     9121 tgcagcagcc ggcccagcag caccacgatt ggcgcgatga ttcctattgt tctatacgca
     9181 ggagtgcacg caatgaccag tgtgccgatc gccattaaaa ataatgtgag gatgagacct
     9241 gtgcgcctgc catgccggtc cacgtacgca ccgagaacga cggcaccgag cggccgcatc
     9301 aggtagcccg caccaaacgt tccaagcgtc atcatcatgg aagcgtatgc gttgctggag
     9361 gggaaaattt cgcgcgcgat gtatgacgca tagtatgcat acaccatgaa gtcatacatc
     9421 tccaaaaaat tgccgctgga gacgcgcata acattgccga ttttcgagcc ggtcgtttgt
     9481 gtgctcatac cacctggttg ttcactatca taccccgctg aggctctttg ctggacagcc
     9541 tcgagcgttt tgcccttgtt aatttcccgc agaatctcca gtcagataaa aatatccgac
     9601 tttgttgttc gatggcgacc cgtaccacat gcgcccttcg gaatccggga agaattcacg
     9661 aattgtattc tccgaatgtg gaaaaggata ttccacaatc ttcccggact taggatccag
     9721 tcggcctaca acatcgagat aatacgagga ataccacacg ctgtgatcgg ccgcgacggc
     9781 tatggcgtac ggaaaagaat cgattccgcc aggcacatcg tattccttaa acgtttcggt
     9841 cttgggatcg aagtggccaa tctttccctt ttgaaattcg ccgaaccaca cggtgccgtc
     9901 cgtatcgagc gccagtctgc gttcgtacga atcgtgagtc ggcggcatcc actgctttac
     9961 tttgaatgtc ttggcattca cttcgccgat ctctccggtt ccgggattcg tgaaccagac
    10021 gttttcatcc ttgtcgaagg caagactgta cgtgtggtct gcctctggaa aatcgtagta
    10081 ttttttcgtt tcggggtcga acctcgtcag cgggtagccg ctcgaccaaa ccatgccctt
    10141 cgaatcaatt cgcacagtat gccgcgagcc tccgtcttcc cgtccttctt tgcccgggcg
    10201 gtacgcgtcc tgatattccg tgatcttctg cgtcacggga tcccaccggc ccaatttatc
    10261 ggaagcctgc tcggtcagcc acacggaacc atccggcgcg gcaatggcgg aatgaactgc
    10321 cgccgttcct tcgttcggaa cagggaaatc ctgcatttcg ccggtcttgg gattcagccg
    10381 cgtaatttta ttcccaattc cgaagttcgg aatccaaacc gatccatctt ttgccggtgc
    10441 ggcgctgaac ggcatgcggc tcggacccgg catgtcatat tccacgtacg tgattttcat
    10501 ggcttcgctc gtgaagggtc ggagcgtttc cttgtacccg ggcgcgtccg cggcggactt
    10561 aggcaggacc gaatccggcc catacaactt ggttaaatag gttgccacct cgtccgcgtc
    10621 ctgatcggta attcgataag acaagctaaa gtgcatcgtc tcgcgcataa actgcacgcg
    10681 atctttccag ccatccgcgt cgcgcgtcac ggatgccatg cgagtctgaa atccgtggca
    10741 gataaagcat ttcgtaaaaa tggtcgtctt ggcgctctct tccggccaaa gttttttcgc
    10801 ctgataaatg ttgagttcat tccaacgcac aatgcccggc tgcatggaaa aatcggccgc
    10861 aaaattttgg tccgccgtca gcgcaacgcc gcttttgggt tccgcgcgaa agcccgtggc
    10921 cttggcctgc atccggtaat cacccgcggg caatttgtcg acgcggtaat ggccctgatg
    10981 atcagacaaa acatagaagc tgattttcgt tttggtgttt tgcgtttgca caaatgcgcc
    11041 ttcgatgggc gctccatcct gccccttcac agtcccggag acggtcgctc catagcagga
    11101 ggacgcgagg tacagcgcaa ggccaaaaac ggccacgaac ttgaattggg accgcatgct
    11161 cactcctcga tgatcaattc cgtatatgcc aatccgaatt ttgaaaccgt gattcgcatt
    11221 atgccccata tgcccggaaa atggatactc cgttcgtttt tttggcaatg tcttaatttg
    11281 aaaatttcta gaatttagga gggccgagtt tcaactcggc catttggttg cctctcgtcc
    11341 ggggcttcag cccctgaagc ttcaggggcg gttaaaaccc cgatcaaacc aagctcgtcg
    11401 tcggggaccg aagtcccgac ctcctaagga accgtgccac gcgaattctt ttcaaattac
    11461 gacactaccc atcttttgct tggccgaagc gtctactgcc tcaaaatccc gtaccaccca
    11521 agaatttttg caaccaattt cggacgtctg tgaaaacgca catagactgt gatggacatc
    11581 gcatacaatc caaaacaatt ccgctaattt cgagtgtttt attaattaat tcaactactt
    11641 gcgcggttac tgatctagtg gtaggtttct cgcaatagcg gttaacactg tagcctctgg
    11701 ccttggctga gcgcctcagt ttctgtttaa ggcagtttca gcatgacctc cggcttggag
    11761 ggtcttcatg cgggtaaaac cccgtttccc cggaattcgc gttacggcca atggaaatca
    11821 attagtctca tatcacacgg aaactagaat tacggatgca ggtattttct atcctattac
    11881 tccgtccact gagggcggcg aactctacca acaggcttat gcggaaggca aattaaatgt
    11941 cttcggccgc agcaccatcg ccgtcgaggc ggaaggcgag cacgcggcgc agggcggagc
    12001 catcgctcac tctgtgtgcg gccagcgggt cgtcaatttc acgtcgggcc agggaattgt
    12061 ctacggcgtc gagcagtatt accacgcgcc gggcaagtgc tcgaccatgg tgctcgaagt
    12121 gggcgcccgc gccctgacca agcatgcgct gaacgtgcac tgcgggcatg acgatgtgta
    12181 cggctcgctc gacacgggat ggatcactct gtttggcaaa gacgctcagc aggccgccga
    12241 tcaggctctg attttgcgcc gtgtaacgga actaagcctc accccgggaa tgaacgtgca
    12301 ggatggattc ctgacttccc atcttgagcg cacattttat aaacacgagt ccgatttgat
    12361 gcgcgaatat ctgggagcgc cggacgacat gatcgagtgt cccaccgaaa gccagcgcgt
    12421 tctctttggc ccgacgcgca ggcgcgtgcc gcgcatgatc gacctgacga atcctgtgct
    12481 gctcggcccc gtgcagaatc aagagcatta catgcaggga gttgtggctc gccgcaacaa
    12541 tttcaccgag ccgattcttc aattcctgga agatgcctac aaagtgtttg gcgacttgac
    12601 cggccgctac tatggcttgg ttacccaata caaaacggag gacgcggaca ccgtatttgt
    12661 ttcgctcggt tccgctgccg aaaatattga ggccggcgtc gactatttgc gggaacatcg
    12721 caacgcaaaa gtcggctcca ttcacctcaa tgtgattcgt cccttccccg aagccgccgt
    12781 cattacggcc attaagggca aacgcaatgt gatcgtcctc gagcgcacgg atgaacccat
    12841 ggccggagac aaccccatgg gccgcgatat ccgcaccgcg ctcagcaagg cgctcgttac
    12901 gcatgaccat gccgcggaag cgaaccttcc ggccatcacg cccgaaacaa tgccgcgcct
    12961 gttcaacggc atttacggat tgggttcacg cgatttccgt cccgaacaca ttctgggcgc
    13021 atacgaattc gccaccggaa cgcgcgctcg caaagacggt aaaggcattg ccgacggaat
    13081 ttcgtatttc gttctgggcg tcgatcaccc gtatgaggtg aaatcggatg agacgccttc
    13141 cctgctgccg gacaaatccg tggctgtacg tttccattcc atcgggggtt ggggcgcaat
    13201 caccaccgga aaaaatcttg gcgcaattat cggcgacctg aacgatctgc tctacgatcg
    13261 tgacaaagtt gtcgacgaat ttggcaatcc aaaggaaatc attcacgtca gcgccaatcc
    13321 gaaatacgga tcggaaaaga aaggcgcgcc gacttcctac ttcatggtcg ccgctccgca
    13381 gcgtattcga gtgaattgcg atctgcgcca cgtcacggtt gtgctttgct gcgatcccaa
    13441 agcgttcacg cacaccaatc cactggacgg aatcctcgaa ggcggctgcc tcatctggga
    13501 gtcggaagag gaaggcgaga aggcctggga gcgcctgccg atgtgggcgc gcaaacaaat
    13561 catcgacaaa aatatccgcg tgtacacgtt gcccggcttc gaaatagcgc gcaaagctac
    13621 ggaccgcgct gacctgcaac tcagaatgca gggtaacgca tttcttggcg catttttttc
    13681 cgtatcgcca attttgcggg agtttgggat tacaggcgaa cagttccgcg aagtcgtgca
    13741 caagcaatac gtaaagaaat tcggccgact gggcgacgcg gttgtgaatt ccaacatgga
    13801 agtcatgacg cagggcttcg agcgcgtgcg cgaaatccgt gtcggcgaaa tgactgcccc
    13861 ggaccattct tcactgcgag gcgaagcact tctccccatg ctcgccgcac acacggacgg
    13921 cggcggatgc ggttcaggat gccgttcgca cgaagtccct gcaggccagg atccgcgcac
    13981 gcctctctca acgattgcca agttcgacgc ggaattccgc tcgaactacg gatacaacca
    14041 gcccgcctcg ccgctcgcct cgctcggcgt aatggctgcc ggcagcggcg acactgcttc
    14101 caaatacgtc gcccgccgcg aaacgccgct ctacatcccg gaaaattgca cgcagtgcat
    14161 ggagtgcatc gccgtctgcc cggatacggc gctgccgaat tgctcgcagg atctcgagac
    14221 tgttttgcgc accgcaatcg gcaactacgt tgcggatccg aacgaacgct ctaagatgct
    14281 tcgtcttgtt cctgaaatcg ataagcggac ccacgcactc atgcgcgatt cgctcgcggc
    14341 gaaggcgctc actcccctgc cggaaattct gcgcaaagtg acgaatgaag tggacggatt
    14401 ttccgatcaa gccaaatgcg aattttttgc cgtcatcgaa aaggttccca tggccttcca
    14461 aaaggtcaac gccatctttt cgacgccgga acgcaaaacg ccgggagccg gcggcatttt
    14521 ctccatcttc gtttcagact tgtgcaaagg atgcgcagcg tgcgtcaccg cttgcggcga
    14581 tcacaacgcg ctcaggatgg tgcaggagac ggaacaggtg aacgccgagc atgaaaccgg
    14641 cacagccttc ctgaacctct tgcccgatac ctcgcagaaa tatctcggcc tctataatga
    14701 tacgcacccg gatgaatcca agaccgctgc gttgcgcaat ttactgatgg tgcggcgcaa
    14761 ctatgacgca ctcgtggcgg gagatggcgc ctgcgcaggc tgcggggaaa agagcatcct
    14821 gaaggcgatt acatccgtca cggaagccta catgcggccg atctaccacg caaaagccaa
    14881 ccgactacga acgaaagcag gcgatctgga acaagcgggc acgcagaaac tggctgaatt
    14941 gaaagctcgc aacccgaagg aatacgaatt gctgcggcaa gccatcgctc atctcgtcat
    15001 gggtctcggc ggcgaggacg aaaaagacac caggaagcgc atcgccgctc acggcgaaat
    15061 caccgacagc gatctcgtcc attccattgc caccgtgatg ctgcaagaag cgttcaacca
    15121 catgaacctg caggccatcg atgggcgtct tgcgaatggc atgtccgtaa tggccatggc
    15181 ggctcacacg ggctgcaaca ccgtttatgg ctccactccc tcgaacaatc cgcacccgta
    15241 tccgtggatg aattccctct tccaggacgg cgtcaccgtt ggttggctgc tgggcgagag
    15301 tttcatcgtg gatcatgccc accgctctgt aatccccgag cgcttggcgg acgtgctgat
    15361 gcagcgcgcg gaaaatgtga tcaacgatcg cgaatattac gagtactcgc atttcaccga
    15421 cgccttgatg accgaccagg aaatccagga acttccgaag gtgtgggtcg taggcggtga
    15481 cggaggcatg ggcgacatcg gatatcaaaa tacttcgaaa gtaattctcc agaatcgtcc
    15541 aaacgtcaaa gcgctgatgc tcgatacgca ggtctattcg aacactggcg gccagaactc
    15601 agactcaact cccatgctcg gcggcaatga catgaatgtg tttggcgcgg ccacgcaagg
    15661 caagaatgtc gagaagaaaa ccgttgccga aacattcctc gccggacacg ggtcgccatt
    15721 cctcgcccag atttccatcg caaatgctcc gaagctctat cgcgccatcc tcgatggact
    15781 ggaatatcgc ggcaccgctt tcctgcaatg cttcactacc tgccaaccgg aacacggcgt
    15841 ggccgatgat atggccctca cgcaagcgca acgagtacgt gactcgcgtg gagcgccgga
    15901 attcatcttc aaccctcgcc tcggcgaaac gtacaacgaa gcgctcgacc tgaagggaaa
    15961 tccttctctc gaaaaagact ggtacgaaac aaaattcaaa acgaccaacg agccctaccg
    16021 ctataccgtg gcccactggt gcgcaaccga agcgcgcttc cgtaaccatt taaagaaaat
    16081 caagaaggaa gacgcggcca agctcattcc tctcgaaaat atgctcgttc gaatcacgca
    16141 gcaggacgtg gtctaccgcc gctacttgca tccggaaaac cgctcgttca ttccggactt
    16201 tggggtctac atcaaggtgc agggttcgaa cggggaaatc gagtaccgtt cgctctcgcg
    16261 gcaacttgtg ctcttctgcg tggaacgccg caaatcctgg cgaatgatgc agagcaaatc
    16321 cggaatcgag aacaaggaat acaaggcgca gcgctccatt ctggccgatg tcgacgcggg
    16381 caaaatctca tgcgaagatt tcttcgcacg agcggaagaa ttgatgaagg agagaacgcc
    16441 cagcgcgcca gagcacgctc cccggcctgc cgcaccggca gttgcgcagg caaccgcgcc
    16501 tttgaaggcg gcggcaaccg caccagcagt tgcggctaag gcgcaagcca aagtatggga
    16561 ggtggtcaca aagagcgttg cgatccatcc gccaaaacca gtgactgtgg ccagcaaacc
    16621 ctttcaagct gccgaaaaga aggaactgga agatgattcg gccgagtggg aagaacgtgt
    16681 gcaaatacgt ctgatgcagc ttgacgtcga tcccggcaac gacgccgatc agggagtcat
    16741 caccaagagc ggcgactgat tatacgtttt atacgctcgg aatttgccaa tcctcccgct
    16801 gggcaggaag gaagcggcgg tcggcatgta gaaaagtact cgaaacaatc ttgaaggaaa
    16861 atacgattcc ggcttagccg gggtatgtgc cggatatgta gctgttcagt tgcaagatcg
    16921 tgtgctcgtg tgcggatata atccatttca ccatatcccc aattgaaact acacccaaga
    16981 tagccccgtt ccctaccacg ggaaggtgac ggatgtgttt ttcggtcatg attctcatgg
    17041 cgtcttccac ggtttgctcg ggcgtcacga acaccgcagg gctggtcata acttccttga
    17101 cttgtgtttc ctttgaagac ttcccctgca aaatcacttt tcgcgcataa tcgcgctcgg
    17161 aaaccacgcc cacaagttcg ccgtctgaaa cgactagtaa tgctcccaca tgcttttcag
    17221 ccatcatttc gatagcgtca taaacgcacg cttcgggcga aatatgccag acattttggc
    17281 ccttctgttt tagaagcaat ccaatgctgt cgatgaggtt cattgccttc tttcctaagc
    17341 tacggaagat cgactgggag gctaccaaac ttctcgatac ctattacccg caaacataag
    17401 tccgctaatt tgccatgtca aggccaaata ttcgggagaa aaaacgcaat cgatgtaacg
    17461 attgccccgc attgtgcgtc tgtcgaggca aggatatagt aaggcacaga aagatacacg
    17521 gtcaaaatat gtcgattcgg gggaagctac agatgaaatc attccgttgg attttcgctg
    17581 tcctgattgt tctactttgg tcctcactga attcgaacgc gcagaccaca aagggcgctg
    17641 tcgattacga agaacattgc gcctcgtgcc acggaaaccc aaaaagcgcg actccagccc
    17701 cagacatcat gtcactgtgg aaattaaccg cggaagacat ctatgcagca cgggagaaag
    17761 gcgcgcacag taccctccag ggcgctacag acgacaactg gcgcgagatc gccttttatc
    17821 tgggccgccg ccgcgtggac atcgcaaaag tcgcggatgc cagactgatg cctaatcagt
    17881 gccccggaaa ctctcctatc ggcgatctgt ccgcaaagcc cctctggaac ggatggggca
    17941 acggcatcgc caatactcgc ttccagtccg cgaaagaagc aggcttgacg gctgcgcagg
    18001 tgccaaatct caaattgaag tgggcgttcg gtttccctgg cgcggatgaa gtttacggcc
    18061 agcctacagt ggctggtgga cgcgtatttc ttggcgtcga tacgggcgct gtttattcca
    18121 tcgatgcgga aaccggttgc gtctattggt cgtatcaggc ggacgcgggc gttcgaaccg
    18181 cgatcaccat cagcccgcta aagggaaaag gagcggccaa gtacggcgtc tacttcggtg
    18241 acatccgcgc gaacgtttat atggttgatg cggctacagg caaagagata tggaaagtaa
    18301 aagtggaaga tcaccctgtt gcgaaggtta cgggtgcgcc aacgttgtat gaagatcgcc
    18361 tgtacgttcc ggtctcttca caggaagagc gctccgccgg atggagcacg gtatatccgt
    18421 gctgcacatt tcgaggcagt gttgtagccc tcgatgccag taatggccac acaatctgga
    18481 agacttacgt tgtccaggat ccaccgaagc ccacgggaaa aactcccagt ggggtacagc
    18541 aatacgggcc gaatggcggg gccgtttgga actcgccaac cattgacccg aagaaccacg
    18601 ccctttatat cggcacggga gactcctaca cgaagcctgt ttcgaagaac accgatgcga
    18661 ttgtggcgct cgacttgaag acgggcaaaa tcatgtggtc aattcaggac acggaaaacg
    18721 acgcatggct ttccggctgc ggcggcgaaa taaagtcaga aaattgcctg ccgccaggcg
    18781 aaattggtcc ggactatgac tttggggcgt caccgattct ggtgtcgcta gggaacggcc
    18841 acagaatgct ggtggctggc cagaagagcg gcatggtatg ggggcacgat ccagaccggg
    18901 aaggaaccgt ggtctggaaa gcgcagctcg tgaagaaact cgcacttggg atgattacat
    18961 tcggcggagc ctcggacgag cagaactctt acttcggact tcgcaccggc ggcgtcgccg
    19021 ctgtgcgaaa tgacaccggc gaacagaaat ggtttacgcc agttcctcca ccgccgggac
    19081 ccggtccaaa gggtgaaaat gcagccatca ccgcgatccc aggagcgatc ttttccggag
    19141 gatgggacgg catgttgcgc gccttctcca cggaggacgg gcatttacta tgggattaca
    19201 atatggtacg tagttacgaa acagtaaatc acgtcgcggc gaagggcggt tcgatggcgg
    19261 cggccggccc aaccgtcgcc ggcggcatgc tgtttgtcgg ctccggctat gtgttcggag
    19321 gcggggtgcc gggcaacgcg ctgctggttt ttgccgcgca atagtccgcg cttttaattg
    19381 agcgggcttc gattttgagc gggataggct ttcacctgtg aggttttaga tcttgcaagg
    19441 actaacttcc acaggctgaa ggctatgcca cgaatgcctt gttcgtcgct tcctaaaata
    19501 tcttttcatg ttttcctgag accaacaaat cacctcccaa accgaaaggc gaaaaattgc
    19561 agcacttcgg cgcgttcgtt atcggcatag cgatcggttt tctgggaggt ttatttggaa
    19621 aaggaggcag cgcggtcgcc acgcctcttt tgagtctcat tggctttccc ggatttatcg
    19681 ccgttgcttc tcctctgccg gccacaattc ctggcacact gatcgcgtcc gccgaatact
    19741 ggcaatccaa actaatcgat tggcaaattg ttttatggag tattggcgtg ggaattcccg
    19801 caaccacatt cggttccatg attacggaat tcactggcgc gcggatcctg ctgattctta
    19861 ccgcgatcct ggtgctcgga t
//
LOCUS       DASZMB010000105         6803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2576272, whole genome shotgun sequence.
ACCESSION   DASZMB010000105 DASZMB010000000
VERSION     DASZMB010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2576272"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VG272_04785"
     CDS             <1..270
                     /locus_tag="VG272_04785"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWF13031.1"
                     /translation="IRELENLIERAVIVSPGPVLRVPLSELRSGAEPAVEGQTLRAVE
                     REHILKTLEGTKWVLAGPHGAAARLGLKRTTLQSKMRKLGVKKQT"
     gene            768..953
                     /locus_tag="VG272_04790"
     CDS             768..953
                     /locus_tag="VG272_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13032.1"
                     /translation="MPEEKAGMALYVLTASGDQPSQLTIGHQTAATASKQKELLNLFE
                     GLAIESAYKRGSAIRYS"
     gene            967..1611
                     /locus_tag="VG272_04795"
     CDS             967..1611
                     /locus_tag="VG272_04795"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF13033.1"
                     /translation="MSQERHLVEGNTAEVSIDEKTEDQLIELGLNGDARALDALFARN
                     TRALYQTAFRVLGNPEDAEEALQEGLLSAYRNLPKFERRSQFSTWLTRIVINAALMRR
                     RSKRSRPAVSLDDFAQEGDVPLAERFADDGPNPEQLYAGTELRDRMKKKLAEISPLLR
                     SAFWLREIEGLSAEEAAHVLGVSRNTLKARLWRARQELAARLGLALDPNLAPAT"
     gene            complement(1707..3224)
                     /locus_tag="VG272_04800"
     CDS             complement(1707..3224)
                     /locus_tag="VG272_04800"
                     /inference="COORDINATES: protein motif:HMM:NF017493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HWF13034.1"
                     /translation="MEFLASSLLNLITETSTNLPPDVRAAMSIAVKEEKPATQSAQAL
                     GIIMSNIDMAVDDTGPICQDTGMPTFYVHTPVGVNQIRIKKAILEAVAEATRLGKLRP
                     NSVDSLTGENSGNNLGEETPVIHFEQWEEDDVEVKLILKGGGCENKNIQYSVPCTLEH
                     LGRADRNLEGVRKCILHAIWQAQGQGCAPGAVGVCIGSDRAHGYELAKGQLFRTLDDV
                     NANPTLAKLEAEIMEEANKLGVGAMGFGGNVSLIGCKVVAANRLPASFFVSVAYDCWA
                     FRRLGVRLDAKSGAINNWLYRDPDRPVQRMAEGSGFELTGREVVLTAPLTEAQMRALK
                     VGDIVLINGEMRTGRDNVHAYLMKNPPPVDLNGSVLYHCGPVMLKQGDEWIVKAAGPT
                     TSIREEPYQADVIKKYGVRAVIGKGGMGAKTSAALKEFGAVYLNAIGGAAQYYARTVE
                     KVLGVDLMEFGVPEAMWHLRVKNFAAIVTMDAHGNSLHADVEKSTGEALETLQKV"
     gene            complement(3944..4498)
                     /locus_tag="VG272_04805"
     CDS             complement(3944..4498)
                     /locus_tag="VG272_04805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWF13035.1"
                     /translation="MEKVRKGEIPWTRLDDLHRMILDDLLKEFKIEGLTEEEKVSWTH
                     VWRRLKPWPDSVEGLARLKKKYVIAPLSNGNIALMTSLAKFGGLPWDAILGAELAHHY
                     KPDREVYVSAYYYLDLKPEEVMMCAAHAPDLQAARSSGLRTGFIYRPNEYGNGPVGVP
                     STAKPGDFDVVSVSMVDLAQQMGA"
     gene            complement(4438..4782)
                     /locus_tag="VG272_04810"
     CDS             complement(4438..4782)
                     /locus_tag="VG272_04810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13036.1"
                     /translation="MRLMVDRRNFLAAAGVAAMSFSELRSVLAKQAPRDMQGANPKLS
                     NVKALVFDTFGTVVDWRSSIAAEGMAWGKAKGLDINWVDFAAAGASDTTRQWKRCVKA
                     KSPGPGSTTFTA"
     gene            complement(4857..5390)
                     /locus_tag="VG272_04815"
     CDS             complement(4857..5390)
                     /locus_tag="VG272_04815"
                     /inference="COORDINATES: protein motif:HMM:NF023010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HWF13037.1"
                     /translation="MRVITAMLICLGLTISGPPAFAQNSRSAAPSKASAAIGALPAPA
                     FEYLGTLHAETGSRAVVENGPQGTRTIVQIVGGRFEGPRVNASVQTPSGDWITNRPDG
                     SYRLDVRPTLKTDDGALLLVTYNGIGHTTQAGASLRAAPLFETGDARYTWLTGLQTIA
                     VGERVGTTLTYDFYALK"
     gene            5779..6102
                     /locus_tag="VG272_04820"
     CDS             5779..6102
                     /locus_tag="VG272_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13038.1"
                     /translation="MVTVALWVRLEAKKGKEKDVEDFLKSGLSIVEAEQGTITWFAIR
                     VGPSTFGIFDTFQNEAGRNAHLNGKVAAALKEKAAELFSEPPSIEKVDVLAAKLANQA
                     SRAGA"
     gene            complement(6189..>6803)
                     /locus_tag="VG272_04825"
     CDS             complement(6189..>6803)
                     /locus_tag="VG272_04825"
                     /inference="COORDINATES: protein motif:HMM:NF019948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HWF13039.1"
                     /translation="VLGKTSGLFAEAVLQRFPQLAVEEDGRLLNLRLREHFLTRIFSF
                     ARLRALRRSVSMRAIVKFHAAHKYVLLAYSEKKMRQLGKIVANGEKETPRRVMELYSA
                     VFYDALAQPTKTAPQINVLMHALGYFSKELTSREKRHFLDALESYRLGRIPLGSVSNI
                     LWSWALRFECSYLLEQVYFRPFPEGLMATDDSGKGRHSANKSIA"
BASE COUNT         1570 a         1804 c         1871 g         1558 t
ORIGIN      
        1 attcgcgaat tggaaaatct cattgagcga gcagttattg tctcgccggg acccgtgctg
       61 cgagtgccgt tgagcgagct tcgttccgga gcggaacctg cggtcgaggg gcagacgcta
      121 cgcgcagttg agcgcgagca cattttaaag actctggagg gaacgaagtg ggtattggcg
      181 ggcccccacg gcgccgctgc acggctgggc ctgaaacgga caacgttaca gtctaagatg
      241 cggaagctcg gcgttaaaaa gcaaacctag ggatgtgccg acatatgggc atatgccgaa
      301 ataacggcag ccacggtctg gcaaatactt cctaaatctt tgacaaatca tcacattaaa
      361 tcctctaacc aactgtaaaa taagccctta taataagtga ttccatttcg gcatttatgg
      421 cctttcgcat gctacctatc cgcagattgt cttatctttc aggattccgt ctagaaggct
      481 ggagatgttc aaggttactt cacggcaggc cgataatact gagacttggg aactagaggg
      541 taagctctct ggcgagtggg tcacggaact tgagcggtgc tggaggcagc gcaaaacgcc
      601 gctagacgtt cctttagaaa ttcgtctaaa ggcgatcagc tacattgacg cagcaggaaa
      661 gcaacttctc gctcaaatgc ataaagcagg ggttggaatc gtgggctgcg gttgcatgac
      721 taaggcggtc gtcgcggaga tttcgcagcc gttaaattcg aattgaattg ccggaagaga
      781 aggcaggaat ggcgctttat gtgctcactg caagtggaga tcagccgtcg cagctgacca
      841 tcggccacca aacggccgcg acggcgtcta aacaaaaaga gctgcttaat cttttcgagg
      901 gcttggcaat tgaaagcgct tataagcgcg gcagcgccat tcgttattca tgaggggaaa
      961 ataaacatga gtcaggaacg tcatttggtg gagggaaaca ccgctgaagt cagcatcgac
     1021 gagaaaacgg aagatcaact gatagagctg ggtttgaacg gagacgcgcg ggccctcgac
     1081 gcgttgttcg cgcgtaatac gcgcgctttg taccagaccg cgttccgcgt gctgggaaat
     1141 ccggaagacg cggaagaggc cctgcaggaa ggcttgctct ctgcctatcg gaatttgcca
     1201 aagtttgagc gccggtctca gttttcaacc tggctgacgc gcattgtgat taacgcagct
     1261 ttaatgcgtc ggcgcagcaa acgctcccgt ccggcggttt ctcttgacga tttcgctcag
     1321 gagggagacg tgccgctggc cgagcgcttt gccgacgacg gcccaaatcc ggagcaactg
     1381 tatgccggaa cggaacttcg agatcgtatg aaaaagaaat tggcggaaat ttcgcccctt
     1441 ctgcggagcg ctttttggct gagggaaatc gaaggattgt cggcggagga agcggcgcac
     1501 gtccttggag tttctagaaa cacgctgaag gcacgtttgt ggagagcgcg tcaggaatta
     1561 gccgcgaggc tgggcctggc actggatccg aatctcgctc ccgctacttg atttatccat
     1621 tcataaagat ggggcttcat tttggcgaca ttgatatctt atatatatac cgccaagctg
     1681 aagccccatt catacctcta gctccctcat accttttgta gcgtttccaa agcttcgcca
     1741 gtggactttt ctacgtcggc atggaggcta tttccgtgcg cgtccatggt gacaatggcc
     1801 gcgaaatttt ttacgcgcaa gtgccacatc gcttccggaa ctccaaactc catcagatct
     1861 acgccgagca ctttttcaac ggtgcgagcg tagtattgcg ccgcgccgcc aatcgcgttc
     1921 aaatacacgg cgccaaactc tttcagcgcc gcggaagttt ttgcgcccat tccgcctttg
     1981 ccgattacgg cgcgcacacc gtattttttg atgacatccg cctgataagg ctcttcacgg
     2041 atgctggtgg tggggcctgc ggcttttaca atccactcgt cgccctgctt gagcatgacg
     2101 gggccacagt gatagagcac tgacccattc aaatcgactg gcggcggatt tttcatcaaa
     2161 tacgcatgca cattatcgcg gccagtgcgc atttcaccgt tgatcagaac gatgtcaccc
     2221 actttgagtg cgcgcatttg cgcctcggtg agcggggcgg tgagcacaac ttcgcggccc
     2281 gtcaattcaa acccggaacc ttccgccatg cgctgcacgg gacgatcggg atcgcggtag
     2341 agccaattgt tgatcgcgcc cgattttgcg tcgaggcgca cgcctaaccg gcgaaacgcc
     2401 cagcagtcgt acgcgacgga aacaaaaaag ctagccggca ggcgattggc cgcaacaacc
     2461 ttgcatccaa tgagcgaaac atttccccca aatcccatcg cgccgacgcc gagcttgttc
     2521 gcttcttcca tgatttccgc ttcgagcttg gcaagcgtgg gatttgcatt gacgtcatcc
     2581 agcgtgcgga ataattgtcc cttcgcgagt tcataaccat gtgcgcgatc gctgccgatg
     2641 catacgccca cggcgcccgg cgcgcagccc tgtccctgcg cctgccaaat cgcgtggagg
     2701 atacatttgc gaaccccttc aaggttgcgg tccgcgcggc ccaaatgttc gagcgtgcat
     2761 ggcacggaat actgaatatt tttgttttcg cagccgccac ccttcaggat cagcttcact
     2821 tcgacgtcat cttcttccca ctgttcgaaa tggatgacgg gcgtttcttc gccgagattg
     2881 ttgccgctat tttctccagt cagcgaatcg acggagtttg ggcgaagctt gccgaggcgc
     2941 gtagcttccg cgacggcttc cagaatcgcc tttttgatgc gaatctgatt cacgccgacg
     3001 ggcgtatgaa cgtaaaacgt aggcataccc gtgtcctggc aaataggtcc agtatcgtca
     3061 acggccatgt cgatgttgga catgatgatg ccgagtgcct gcgcggattg cgtcgcgggc
     3121 ttttcttctt tcaccgcgat ggacatcgcc gcgcgcacat cgggcggaag atttgtggag
     3181 gtctcggtaa tcagattgag cagactggaa gccaggaatt ccattttgtt acctccccaa
     3241 atttttaatt ttacactcgt ttgaggagaa ggtctcaact cttcattaag tttcggatca
     3301 ggccacgaag gtcaggctgg ggcgtgcttc ggatgcaaat catttctcga agataagtcc
     3361 ggacaggaag ggtgagtccg tccactcagc cgtcgaaaac cgaacgccgg gactcgcgca
     3421 aattcaccag cactgaaacc ccggccaccg tccgaaagct cacaatatcc ttcaaagaca
     3481 attgtgatga tttcaactac ttcgatcaac aatgttaatt attttgcgtc tctgggaagg
     3541 tgccattgac ggcgcttatc ttcagggcgg cgaaccgcct gttgttttcg atctggtagt
     3601 ccggaatcaa acggcgagga attgcggccc ttatatgagc ctccggattt gttggagttc
     3661 tgcggccgat tcgcaacgga ttttatcgcg ctcgtcaatt tctgcaattc ttctctaatc
     3721 agccgcaact cgcttgcgat gttgtcggcg gtttcatctt tcattttgca tcctttcacc
     3781 ggctcgctcg gagctcggct tcaaatcaac tcgcattgcc aaataatggc agggcgactg
     3841 acatagttta cacggcaggg aaagctttgg ctgcggaact taacacatag aggggtaatt
     3901 cggcctttcg attcacatgt cggctcaaaa aataccgtcg cggttatgcc cccatttgtt
     3961 gagccaaatc gaccatgctc acagaaacca catcaaaatc tccgggtttt gcggtgctag
     4021 gcacaccgac tggcccgttt ccgtattcgt ttgggcgata gatgaatccc gtgcggagac
     4081 cactgctgcg ggcagcctgc aagtcgggag cgtgggcggc gcacatcatc acctcctcag
     4141 gttttagatc gagataatag taagcagaga cgtacacttc gcgatccggt ttgtagtgat
     4201 gggccaactc ggcgcccaga atggcgtccc aaggcaaacc gccgaatttg gccaaactgg
     4261 tcatcagcgc gatattgccg ttcgacaatg gcgcgatcac gtatttcttt tttaagcgcg
     4321 caagtccttc gacagagtca ggccagggtt taagtcgacg ccagacgtga gtccacgaaa
     4381 ctttttcttc ttcggtgagc ccttcgattt tgaattcctt gagcaaatca tccaaaatca
     4441 tgcggtgaag gtcgtcgagc ctggtccagg ggatttcgcc tttacgcacc ttttccattg
     4501 ccgggtggta tccgaggcgc cagcggcagc aaaatccacc cagttaatgt ccagcccttt
     4561 tgccttgccc catgccatgc cctccgcagc tatcgaactg cgccaatcca caaccgtgcc
     4621 gaacgtatcg aacacgagtg ccttcacgtt ggacaattta gggtttgctc cctgcatgtc
     4681 gcgcggcgcc tgcttggcga gaacggaccg aagttcagag aacgacatcg cggcgacacc
     4741 ggcagccgcc agaaaatttc ggcgatcgac catgagacgc atattaagcc acctccttgc
     4801 gaattgaagt tgctgattga gcgctgagat cttcccgcga tgaaactgat tgcacgctac
     4861 ttaagcgcgt aaaagtcgta tgtgagcgtt gtgccaacgc gctcgccgac ggctatggtc
     4921 tgcaatccgg tcagccacgt gtagcgcgcg tcgccggtct caaagagagg ggctgcgcgc
     4981 agggatgcgc cggcttgtgt tgtatggccg atgccgttat aggtcaccaa aagcagcgcg
     5041 ccgtcatccg tttttagcgt tgggcgaacg tcgaggcggt agctgccgtc ggggcggttg
     5101 gtaatccagt ctccggacgg cgtctgtacc gacgcgttga cgcgcggacc ttcgaaacgg
     5161 ccgccaacga tctgcacgat agtgcgggtg ccttgcggac cgttttcgac gacggcgcgc
     5221 gaacccgttt cggcgtgcag cgtgccaagg tattcgaaag caggagcggg aagagccccg
     5281 attgccgcag aagctttcga aggggccgca ctcctggaat tctgagcgaa cgcgggtgga
     5341 ccggagatcg tcagcccgag gcagatcagc atcgcagtaa tgacacgcat cgtccctcct
     5401 gaatggaatg gctgattata gctgcgtcaa cacgggcaat acgaggtgaa ttgcgtcagg
     5461 gaactgtatt gaggagctgc cgcagatcgc ggtgccgaaa atcgttgtat ccgaggaatc
     5521 gactgcttcg acgcgatttg atcttgtaga gtgaaacttg agtgtcacgc acttgagccg
     5581 cgcggtgcaa acgagcgctg attcaaatca gcacacccaa ttctctcgct gtccggattt
     5641 cctttcattc aggcgtcgca tctcactacg atgagggcga tgcgattgcg gctctaatcg
     5701 aagcaatctc aggtgagaca cgaatttcgc ccgcgaagta taaattcgag aatgcatcca
     5761 ttcggaagga gaaatcaaat ggtaaccgta gcgctttggg ttcggctgga agcaaaaaaa
     5821 ggaaaagaaa aagatgtgga agatttcctg aagagcggac tttcaattgt tgaggcggaa
     5881 cagggaacca ttacatggtt cgcgattcga gtcggtccat ccacattcgg aatttttgac
     5941 acgtttcaga atgaagcggg acgaaacgca catctgaacg ggaaagttgc ggcggcgctg
     6001 aaggaaaagg cggccgaact cttttcggaa ccgccgtcca tcgaaaaggt cgatgtactg
     6061 gcggcgaaac ttgcaaacca ggcttcacgc gcaggagcct aaactgaatt cgctcccgcg
     6121 tttctcgctt tacggaatca agtgttaaac gtgccgcacc attcaggcaa tggcgcggca
     6181 cgccattttc aagcgatgga tttattggcg gagtgcctgc cttttcctga atcgtcggta
     6241 gccatcagac cctctgggaa tggccgaaaa taaacctgct cgaggaggta gctacactca
     6301 aaacgcaatg cccaggacca caaaatattg ctgacactgc caagcgggat tctgccaagg
     6361 cgataggatt ccagcgcatc caggaagtgg cgcttttcgc gtgatgtcaa ttctttagaa
     6421 aaatatccaa gggcgtgcat gagcacgttg atttgcggcg ctgttttcgt aggctgcgcc
     6481 aacgcatcgt aaaaaaccgc ggaatataat tccatgacgc gccgaggagt ttctttctct
     6541 ccgttggcta cgattttccc cagctgccgc atctttttct cgctgtaagc caacaaaaca
     6601 tatttatggg cagcgtgaaa tttgacaatc gcgcgcatgg aaacggagcg gcgcagcgcg
     6661 cgcagccggg caaaagaaaa aatgcgtgtc agaaaatgtt cgcgaagtct caagttcaaa
     6721 aggcggccgt cttcttctac ggcgagttgt ggaaagcgtt gcagcacggc ttccgcaaac
     6781 agaccagacg tttttcccag cac
//
LOCUS       DASZMB010000106        14077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4493391, whole genome shotgun sequence.
ACCESSION   DASZMB010000106 DASZMB010000000
VERSION     DASZMB010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 14077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14077
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4493391"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VG272_04830"
     CDS             complement(<1..1096)
                     /locus_tag="VG272_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWF13040.1"
                     /translation="MASLTDTGVVPRTAESREGVELSVVMPCLNEAATIGVCVKKVME
                     ALERHGIRGEVIVADNGSTDGSQQIAREMGARVVPVERRGYGSALQNGIAAAHGHFVL
                     MGDADDTYDFAQLNEFVAKLREGYDLVMGNRFKGKIFPGAMPPLHRYLGNPVLTGLGR
                     LFFKSPVGDFHCGLRAFRKDAIERLGLRTLGMEFASEMIVKAAAFGLRVTEIPTTLAP
                     DRRNRPPHLRTWRDGWRHLRFLLLYSPRWLFLYPGMTLFALGIMVNAFLLPGPRRIGN
                     VVFDVHTLLFAAMAILIGFQSVVFATFTKIFAISEGLLPEDPRLTRMFRYITLEVGLI
                     LGIFLILAGAGAWILGLEYWRIREFGALDPE"
     gene            1489..2280
                     /locus_tag="VG272_04835"
     CDS             1489..2280
                     /locus_tag="VG272_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13041.1"
                     /translation="MESNEEDLLAAYNKQVKAVQTIQATVDLIPSTGTTYSGVIEEYH
                     DVPGFILAERPATVRIIGQAPVVAKNIFDMVANEKEFRIYIPSKNSFLVGPTALIRPS
                     KKPLENLRPQHIVESLFWPELPPTANVLFEQFDFNVSRYYILTLLRQAEGGKFAIARK
                     IWYNRTDLLVARVQIFGAGGKLESDIGYSDWQPSPSAPGAPATEQTKFARDIHIWRPQ
                     DDYKLEIKILKITLNDPISPDRFVLTEPPGTDLIHVGDEISKVIR"
     gene            2277..3377
                     /locus_tag="VG272_04840"
     CDS             2277..3377
                     /locus_tag="VG272_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF13042.1"
                     /translation="MIRQLVVNNVLHRPIRTIVSVIAVGVEVALVILIVGLTSGLLQE
                     TAKRIEGIGADIMLQPPSASVFLAFSGAPMPIKIADKLRELKYVQAVAPVLLQFSSSN
                     GVDVIYGIDPKSFRDVSGGFVFLEGHDMQGPDDILMDDWAAKAKNVSVNSTFRLLDHD
                     FHVAGIVEHGKGARIFVQLSTLQDLSGSRDKASVFFMKCTDPNYTQAVMDEMKPIFPG
                     YEIRPLKDFLSLMTATNLPGLNAFIKSMIVLAVSIGFLVILLSMYTTVIERTRDIGVL
                     KSIGASKGYIIRALLGESVFICVIGIGVGIAMSYVTRAAFLSSFPTLSILITPDWIVR
                     SAVIALAGGILGASYPAWLASRKDAIEALSYD"
     gene            3532..4215
                     /locus_tag="VG272_04845"
     CDS             3532..4215
                     /locus_tag="VG272_04845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF13043.1"
                     /translation="MEPIVVTEDLWKLYHAGKVEVPALRGVNFKVMPGEFVSVMGPSG
                     CGKSSLLYVLGGLAQASRGRVLVDGNDLSTMSDAERTKLRRDKVGFVFQRFNLLPTLD
                     ARGNIAIAQHIHGDGFNSSRFEGITNLLGLTERLKHRPSEMSGGEQQRVAIARAIIND
                     PKIILADEPTGNLDTQNSEIVLSMLSQLNKDLGQTIVMITHNPEAAEYSTRVVHMRDG
                     VIVDGVPAA"
     gene            4217..4612
                     /locus_tag="VG272_04850"
     CDS             4217..4612
                     /locus_tag="VG272_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13044.1"
                     /translation="MRTLWRAIVRVLFWSYERGTWQYDLAVAGIVIFVLFSPLVIHYT
                     DQPTVGPAPASALVRLQTVAPGQVSTYRVDARLLATPTRTPELEHELHETVQKNVQDL
                     NHGRFQIMHIEPIRGEGGTIAYYDVSVKP"
     gene            complement(4731..5009)
                     /locus_tag="VG272_04855"
     CDS             complement(4731..5009)
                     /locus_tag="VG272_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13045.1"
                     /translation="MQAKIEVEKFDQSRFKVRVIEGGSESGHQVTLSPKDYAKLGNGT
                     VAPEELIRKSFQFLLEREPKESILGRFDLAIIGRYFPEYEGEIKKRLE"
     gene            complement(5048..6244)
                     /locus_tag="VG272_04860"
     CDS             complement(5048..6244)
                     /locus_tag="VG272_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWF13046.1"
                     /translation="MQRFSGRAALFFLAALALLNTNNPQVRAASPQQNPSAKSQKLPR
                     PVPLGRDLSLERDAASEELRGIAKSSLPAPPANAIRSHVTLVQVPCTVTAADRTQVRG
                     LSQNDFHVYEDGAAQEIASFDASATPASVVLVIDASPSIFHQLSEMRDAARALAINLS
                     PADEISVVSFSSEAHLLLPFSTNRALLERAIDSQYLSEVENSSESRIYESVFLAAHEL
                     FQGRTGRKAIVLLTDGQDSGLGLTWDPRSAKPSTGAAGSRLSFDDVARELGADGIALF
                     IVSTENRPRGMTPEWLEAHRTEMLVNAETRKENMPNYTLYLAELARRAGGQLYFLREV
                     GNLTEIYRRIALAIGAQYTIGFYPSEGTMRPGWRSLAVTLDDAAKVPSGSKLTYRGSY
                     YVSAFQ"
     gene            complement(6351..6656)
                     /locus_tag="VG272_04865"
     CDS             complement(6351..6656)
                     /locus_tag="VG272_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13047.1"
                     /translation="MPKNPAPTIEVTCPCCESKLTVDTDLAVVLAHTTPVRAAPDVDI
                     NDAARILADQARKREDKFRDSWEAESKKEDVLNRKFEEALKKAKDQPAEKPLRDFDL"
     gene            6778..7326
                     /locus_tag="VG272_04870"
     CDS             6778..7326
                     /locus_tag="VG272_04870"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HWF13048.1"
                     /translation="MALEFTTSCLKDSVDLLHFYKKLGDRAMSQVSDDGLFEVLDNES
                     NSIAIIVKHLAGNMRSRWTDFLNSDGEKTDRDRDSEFETPPKTRDELLALWESGWKLA
                     FEALAPLTDADLSRTVYIRSEAHSVMQTIHRQVAHYSYHVGQIVYLAKHFAGPDWNAL
                     TIPRRKSAEYNSSVASGKLSQR"
     gene            7745..8794
                     /locus_tag="VG272_04875"
     CDS             7745..8794
                     /locus_tag="VG272_04875"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF13049.1"
                     /translation="MGCGCGFTRREVLRAGSLAAAGLLTGATLGAQAKKGMRIDVHAH
                     LWNDEYLDLCQSYGNVDTGTQRNKGAGMGQAEMEKRFAAMDANGVEMQILDICPQAPS
                     FENKEHAVKAARMANDLYADAVSRWPKKFKAFAAVPLPHVDEALKELERALDQLKFVG
                     ATITTFIGNRSVADPAFAPFYEELNRRASVLYIHPSGNGVDSPFVIPYNLRWAIGAPM
                     EDTVSIMHLIEAGIPQKYPKLKIINSHLGGMIPMVFQRLDNISKWEHPLPELPTVTAK
                     RMWYCSVGHGHPPALRAAVDSMGADRICLGSDFPYEAGELYNRAIQYISDSGLKPGDA
                     EKILDRNAAHVLGLA"
     gene            8791..10101
                     /locus_tag="VG272_04880"
     CDS             8791..10101
                     /locus_tag="VG272_04880"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF13050.1"
                     /translation="MTTTQTPITPAGKPRLTRNQVRSFWAAWGGWSMDGMDSFIYSLV
                     LVPALTELLPRSGIPATPANVGYYGGIFFTLFLIGYGTALIWGPIADRFGRVRTMMFS
                     ILCFSLFTLLAAFATGVWSLAMFRFMAGVGIGGEWSIGASLVSEDWPEERRTMGAALM
                     HTGYYIGFFLAGIANIFIGSHYGWRAMFALGGVPALLIGLIRNNVQEPARWENKVQEL
                     GAKFTMHRAFLKLFSPLYRRRTIVNSVYLIVSLAGLWAGSVYAPTAMTYIAQRAGKTA
                     AESARLASYSTALLGVATVLGALIVPFIADWLGRRATLAIFYVVMMFAIWLAFAHVFY
                     MQQNAIAWFLVCSALLGLGGANFIVYSFWLPEQYGTECRASAFAFITNLGRFFAAGFT
                     FLVGAGIRHFQTFGIPVAMMALAFVVGLALVPFGEETKGKRLPA"
     gene            complement(10230..10694)
                     /gene="bcp"
                     /locus_tag="VG272_04885"
     CDS             complement(10230..10694)
                     /gene="bcp"
                     /locus_tag="VG272_04885"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HWF13051.1"
                     /translation="MLKESDPAPDFRLPADDGSEIALKDLRGKHVALYFYPKASTPGC
                     TTEAIEFRDLKKDFDKLNAIILGCSADAVAALAKFKAKQRLNFPLLSDPNFTVIEAYD
                     ARRMKSFLGKSFLGIVRSTVLVGPDGKIEKIWSTAKSKGHAAEIVEALKTLQ"
     gene            complement(10820..12307)
                     /gene="gatB"
                     /locus_tag="VG272_04890"
     CDS             complement(10820..12307)
                     /gene="gatB"
                     /locus_tag="VG272_04890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HWF13052.1"
                     /translation="MAKISQEGLTSISPEITAAYEPVIGLEVHVQLKTLTKAFCACST
                     RFGDPPNSNTCPVCLGLPGALPVLNKHAVELGIRAALALHLNVQDFSRFARKNYFYPD
                     LPKGYQISMYELPLAADGYLEIEHDGQIKRIGITRLHLEDDAAKNLHEGFPDSSEKSY
                     VDYNRGGTPLAEIVSEPDLRTPTEAHAYLTALKEVMLYTEVSDCNMEEGSLRCDANVS
                     VRRRGAEKFGTKAEVKNLNSFRHLQHALEYEIERQIGVIEAGGKIVQETRLWNVAAGR
                     TESMRSKEFAHDYRYFPEPDLLPLRVSAAWRDAIASTMPELPEAKRQRFVREYGITPY
                     DAGVLASTRELADYFEGVARAGAPAKSAANWIQVELLRQLKEAEKEITESPVQPAELA
                     ALIAKVESGEINAATGKKVFARMFATGKTAAEIIVAEGFAQVSDTGEIERWCREVIEK
                     NPDNLAKYRAGNEGVFKFFVGQVMRVSRGQANPQTVNDTLKRLLS"
     gene            12606..13790
                     /locus_tag="VG272_04895"
     CDS             12606..13790
                     /locus_tag="VG272_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HWF13053.1"
                     /translation="MRKLLNPRSFLFLLLLLIIPSASFAQVAISVAIGPPALPVYAQP
                     PCPTEGYMWTPGYWGWRAEGYYWVPGVWVAPPRVGVFWTPGYWGLAGGLYGWHAGYWG
                     PHVGFYGGINYGFGYGGVGFWGGRWAGEHFAYNTAVMNVNTTVIHNTYVDRTYVNNTN
                     VVNNHTSFNGEGGVNARPTATEQAAEHENHFQPTSNQLSHEQTARGDNNQLASVNHGR
                     PGNAAMDSVNGRRYNQQGRIANGVSSGQLTAGETKNLEGREAGLNKEIHNDRSANGGT
                     LTPRERQQVNGQQNNLSKSIYNDKHNANTANYGNNEVGARRDMQQQRVANGIRSGQMS
                     PSEAAKTEGREQNINRSVAADRQANGGKLTPGEKQNINRRQNGTSRQIYNEKHNEKTA
                     PR"
BASE COUNT         3137 a         3870 c         3786 g         3284 t
ORIGIN      
        1 tttccgggtc aagcgcgcca aactcacgaa ttcgccagta ttccaggcca aggatccatg
       61 cgcccgcccc tgcaagaatc agaaaaatcc ccagaatcag gcccacttcc agtgtaatgt
      121 agcgaaacat tcgcgtcaga cgcggatctt ccggcagcaa tccctcggag attgcaaaaa
      181 ttttggtgaa tgtagcgaaa actacggatt gaaatccgat cagaatcgcc atcgccgcaa
      241 acagaagtgt gtgaacgtca aataccacgt tgccaattct tcgcggtccc ggcaacagga
      301 acgcatttac catgattccc aaagcgaaga gcgtcattcc cgggtacaaa aataaccagc
      361 gcggactgta cagcaaaaga aaacgcagat gccgccatcc gtcccgccat gtacgcaggt
      421 gcggtggacg attcctgcgg tctggagcaa gtgttgtggg aatttccgtg acgcgcaggc
      481 cgaatgccgc agccttcaca atcatctcac tggcaaactc cattcccagt gtgcgcagac
      541 ccaaccgctc gatggcgtcc ttgcgaaacg cgcgaagccc acagtgaaaa tctcccaccg
      601 gactcttgaa aaacagcctg ccaagcccgg tcagaaccgg attccccaaa tatcggtgca
      661 gcggaggcat cgctcccgga aagattttcc ccttgaaccg gttgcccatc acaaggtcat
      721 atccttcgcg gagtttcgcg acgaactcat tcagctgcgc gaaatcgtat gtgtcatcgg
      781 cgtcccccat cagcacaaag tggccgtggg ctgcagcaat gccgttttgc agcgcgctgc
      841 catagccacg gcgttcgacc ggcaccacac gcgctcccat ctcacgagca atttgttgcg
      901 agccatccgt gctcccgtta tccgcgacga tcacttcgcc gcggatgcca tgccgttcca
      961 aggcctccat cacttttttg acgcacacac cgatggtggc cgcttcattc aagcagggca
     1021 tgacaacgga aagctccact ccctcgcggc tttccgcggt tcgtggaact acacctgtgt
     1081 ccgtcaatga agccatggga ttgaaaacta gcccctctat ttcgctgcaa gctgcatcag
     1141 atggaactct aacccatccc gcacgtggcg gaaagggtcg cccaggcctg tgattcggtg
     1201 gattgacgaa gaacaaaatt ccacactctg tcctcgcgct gttaaaatta cagtcgagat
     1261 aaaagtgccc ctcggacggc gcaaattaaa cagttccccc gcggcgtttc aaattgacac
     1321 ccctcggagc ggttgttaga ttgaatgatt ctacaaaggg agtaagaaac actgagtaaa
     1381 aatccgagaa tctggatcct gctgttggcg ttatttgcca tgcaagcggc ggggtgtatc
     1441 actatcaagc gaagaaccac catcaaaaag gaagaaatcc tccccgagtt ggagtccaac
     1501 gaggaagatc tgctggcggc ctataacaag caggtgaagg ccgtgcaaac cattcaggcg
     1561 acggtagatc tgatccccag cactggaact acctacagtg gtgtcattga ggaataccac
     1621 gacgtccccg gattcattct ggccgagcgc cccgcgacgg tacgcatcat cgggcaggct
     1681 ccggttgttg ctaaaaatat tttcgacatg gtggccaacg agaaggaatt tcggatttat
     1741 attccttcga agaactcatt tttggtcgga cctacagctc tcattcgtcc atcgaaaaag
     1801 ccgctggaaa atttgcgtcc tcagcatatc gttgaatccc ttttctggcc ggaactgccg
     1861 ccgacggcga atgtgctttt cgagcaattc gatttcaatg taagccgcta ttacattctg
     1921 accttgcttc ggcaagccga gggcgggaaa ttcgcaattg caagaaagat ttggtacaac
     1981 cgcaccgacc tgctcgttgc gcgcgtccag attttcggag ctggcgggaa acttgaatca
     2041 gacattggct attcagactg gcagccctcg ccctcagcgc caggcgctcc tgccactgaa
     2101 caaacaaagt ttgcccgtga cattcatatc tggcggcctc aggacgacta caaactggaa
     2161 ataaagattc tgaaaattac actgaatgat ccgatttcgc cggaccggtt tgtgctgacc
     2221 gagcctccgg ggaccgacct cattcatgtg ggtgatgaaa tctcgaaagt catacgatga
     2281 ttcgccaatt ggttgttaac aacgtgcttc atcgtccgat ccgcaccatt gtgagtgtaa
     2341 tcgcggtggg agtggaagtg gcgctggtga tcctgattgt ggggcttacg agcgggttgc
     2401 tgcaggagac cgcgaaacgc atcgagggaa ttggcgcgga catcatgctc cagccgccgt
     2461 cggcatccgt ttttctcgcg ttcagtgggg cccccatgcc gattaaaatc gcagataaac
     2521 tgcgcgaatt aaagtatgtg caggccgtcg ctcccgtcct gctgcaattc agttcctcga
     2581 acggcgtgga tgtaatttat gggatcgatc ccaaatcgtt ccgtgatgtt tccggcggat
     2641 tcgtcttcct ggaagggcat gacatgcagg gtccggacga tatcctgatg gatgattggg
     2701 cggcaaaggc caagaacgta agtgtgaaca gcacctttcg cctgctcgac cacgattttc
     2761 atgtggccgg aattgtggaa catggaaagg gagcacgtat atttgtccaa ctttccactt
     2821 tgcaggatct ttccggatcg cgcgataagg cctccgtttt tttcatgaaa tgcaccgacc
     2881 caaattacac gcaggctgta atggatgaaa tgaagcccat tttccccggc tatgaaatcc
     2941 gtccgctgaa agattttcta tcgctgatga cggcaacaaa tctgccgggc ctgaacgcgt
     3001 tcatcaaaag catgatcgtc cttgccgtct cgatcggttt ccttgtaatt cttctttcta
     3061 tgtacactac ggtcattgag cgcacgcggg atattggcgt cctgaaatcc attggcgcgt
     3121 cgaagggata tatcattcgg gcgctgctcg gtgaatcggt gtttatctgt gtgatcggca
     3181 ttggcgtggg aattgccatg agctatgtca cccgggccgc gtttctatct tcgtttccaa
     3241 cgttatcgat tctgattact ccggattgga ttgtgcggtc cgcggttatt gccctggcgg
     3301 gtggaattct tggcgccagc tatccggcct ggttggccag ccggaaggat gccatcgaag
     3361 cactttccta tgattaatat gatcaagatg attaagagtt ttgcaaaacc ggagcacatt
     3421 gcttccataa tttgagcctg cttattgtgg cctcgggcct agtaagtaat gccaatcgcg
     3481 attacaatac gtccgcctgc cgatgtgctg ctcaccacaa gggatctgag agtggaacca
     3541 atagtagtaa ccgaagattt atggaagctg taccacgccg ggaaagtgga agttccggcg
     3601 ttgcgcggtg ttaattttaa ggtgatgcca ggagagttcg tcagcgtgat gggcccctct
     3661 ggatgcggca agtcatcgct gctctatgtg ctgggcggat tggcgcaggc atcgcggggc
     3721 cgcgtgctgg tcgatggcaa cgatttgagc accatgagcg acgcggagcg gaccaagctg
     3781 cggcgcgaca aagtcggctt tgtgtttcag agattcaatc tgttgcccac gcttgacgcg
     3841 cgtggaaaca tcgccatcgc gcagcacatt catggcgacg gcttcaattc gagccgtttc
     3901 gagggcatta ccaacttgct tggcctcacc gaacgcctga agcacaggcc ctcggaaatg
     3961 tccggcggag aacagcagcg cgttgccatc gcgcgagcca ttatcaatga ccctaagatt
     4021 attctggcgg atgagccgac cggtaactta gacacacaaa attccgaaat tgtgttatcc
     4081 atgttgagcc aattgaacaa agaccttggc cagacgatcg taatgattac tcacaatccg
     4141 gaagcagccg aatattcaac ccgcgtggtt cacatgcgcg atggtgtaat tgttgacgga
     4201 gtgccggccg cctgacatgc gaacgctctg gcgagctatc gtgcgggttt tgttctggtc
     4261 ctatgaacgg ggaacctggc aatacgacct tgccgttgcg gggattgtaa tcttcgttct
     4321 tttctcgccg ctcgtgatcc actacaccga tcaacctact gttggcccgg ccccagcttc
     4381 agccctcgtg cgattgcaga ctgtcgctcc gggccaggtt tccacttatc gcgtggatgc
     4441 gcgcttgctg gctacgccga ctcgaacgcc ggaactcgag cacgaactcc acgaaactgt
     4501 acaaaaaaat gtacaagatt taaaccacgg acgcttccag atcatgcaca tcgaacccat
     4561 ccgtggcgaa ggcggcacca tcgcctacta cgatgtctcc gtaaaaccct gataccacgc
     4621 gattcagata tccccaaaaa ttttaagttc tactgtagct cgcctcttca gagaagaggg
     4681 ttcagatgta agttcgcccg ccgaaaaaat ccgcgcggct gcggacccga ctactccagc
     4741 ctcttcttga tctctccctc atactccggg aaatagcgtc cgatgatcgc caaatcaaac
     4801 cgtcccagaa tcgattcctt aggctcccgc tccagcaaga attgaaacga tttgcgtatc
     4861 aattcttccg gcgcaaccgt gccgtttcca agcttggcat aatctttcgg tgaaagtgtg
     4921 acttgatgcc cggactcgct tccgccttca atcactcgaa ctttgaaacg ggattgatca
     4981 aatttttcta cttcgatttt tgcctgcatg agttctccgt tttatgaagg gcaggctgaa
     5041 gcccgcccta ttgaaacgcg gaaacataat acgagccgcg atatgtgagc tttgaaccag
     5101 aaggaacttt cgcggcgtca tctagcgtga cggcgagcga acgccaaccg gggcgcatgg
     5161 ttccttccga tggataaaag cctatggtgt attgtgcccc gattgcgagc gcaatgcggc
     5221 gataaatttc tgtcaaattt ccaacttcgc gcaggaaata aagttgccct ccggctcgcc
     5281 tagccaactc cgccagataa agcgtgtaat tgggcatatt ttccttccgc gtctccgcat
     5341 tgacgagcat ttcggtgcga tgcgcttcca gccattccgg ggtcattccg cgtggacggt
     5401 tttcggtgga gacgataaat agcgcaatgc catccgcgcc gagttcccgc gccacatcgt
     5461 caaatgagag ccgcgatcct gccgcgcccg tgctgggctt agcgctgcgc gggtcccatg
     5521 tgaggccgag cccgctgtcc tgtccatccg tgagcaagac gatcgccttg cggcctgtgc
     5581 gtccctgaaa taattcgtgc gccgcgagaa ataccgattc atatatccgc gattccgaag
     5641 aattttctac ttcggagaga tattgcgaat cgatggcgcg ctctaacaaa gcgcgattcg
     5701 ttgaaaatgg taaaagcagg tgcgcttccg aagaaaaaga cacgactgaa atttcatccg
     5761 ccggagaaag atttatggcg agagcacgcg ccgcatcgcg catttccgat agttggtgaa
     5821 aaatgctggg gctcgcatcg atcaccagta ctacgctggc aggcgtagcc gatgcatcga
     5881 acgaagcgat ctcctgcgct gcgccatctt cataaacgtg aaaatcattt tgactgagtc
     5941 cgcgcacttg cgtcctatcg gccgccgtta ccgtgcatgg cacttgcacc aaagtgacgt
     6001 gtgagcgaat cgcgttagca ggcggcgcgg gaagcgaact cttcgcgatt ccgcgcaatt
     6061 cttctgacgc tgcatcgcgt tccagtgaca agtcgcgccc aagagggacg ggccgcggca
     6121 gtttttgaga tttcgcggag ggattttgct ggggagatgc agctcttact tgtggattat
     6181 ttgtgttgag cagcgccaat gctgcaagaa aaaaaagagc ggcacgtcca gaaaatcttt
     6241 gcacgtaccc aagtatgcca gattttttaa gtggcgcggg cttcagcctg cggggattag
     6301 gctttgcaag gtccaacccc cgcaggcaaa agcgcgcgct actcgagctc ctacaaatca
     6361 aaatcgcgca aaggcttttc agcgggttga tcttttgctt tcttcagtgc ttcctcgaat
     6421 ttccggttga gcacgtcttc cttcttgctc tcggcttccc atgaatcgcg gaacttgtct
     6481 tcacgcttcc gggcctgatc cgcgagaatt cgcgcggcgt cattgatatc gacgtcaggc
     6541 gcagctctta ctggcgtagt gtgcgccagc accacggcca gatcggtatc caccgtaagt
     6601 ttggattcgc agcaagggca agtcacttca atcgtcggtg cagggttctt tggcataaaa
     6661 acattttacg cccatttcgc cttcgtagca cacagccttc aggctgtgtg gggtttagtc
     6721 caagccaagc tgaactccac gcttgatttc cacagaaacc agcgtcaaaa tccatccatg
     6781 gccctcgaat ttaccacctc atgcttgaaa gattcagttg accttctgca cttttacaaa
     6841 aaattgggcg accgcgccat gtctcaagtt tccgatgacg gtcttttcga ggtcctcgat
     6901 aacgagtcaa attccattgc gattatcgtg aaacatctcg ccggcaacat gcgctctcgc
     6961 tggacggatt ttctcaacag cgacggcgaa aagacggacc gcgatcgaga ttcggaattt
     7021 gagacgccgc cgaagacgcg cgacgagctt ctcgcgctat gggagtccgg ctggaagctg
     7081 gcatttgagg cgctggctcc cctcacggac gcggatcttt ctcgcactgt ttacattcgt
     7141 tccgaggcac attccgtcat gcagaccatt caccgccagg tggcgcacta ttcttaccat
     7201 gtggggcaga tcgtttatct cgccaagcat ttcgccggac cggactggaa tgctttgacc
     7261 atcccgcgtc gcaaatccgc ggaatacaat tccagcgtcg cttcgggaaa attatcgcaa
     7321 cgctagcttt cagtagcgca tttaagtagc acaggcttca gcctgtgggt tttgaattta
     7381 gtgtaagcaa ccccacagcc tgaagagtct gtgtgagaat tgcagaatct ttgtgggtcg
     7441 tggctttagg cacgacataa gtcctgcaaa atgcgtgcgg cttttcagtt tgcggaaaaa
     7501 gtagtttatt ctgtcattcc gagcgaagcg aggaatctct cttcgattta agtctaagaa
     7561 aataaagaag gagggattcc tcgcttggct cggaatgata aaatgttgag tttttccgca
     7621 gcctctttag ccgctgaagc ttcaattttc cagttctcac acagattctg aagcctgtgc
     7681 taccaaatcg ttccatcgta caatgtttcc agacatgtga gtcggccagg gaggaacttc
     7741 cattatggga tgcggatgcg gttttacgcg gcgcgaagtg ctgcgcgcgg gttctttggc
     7801 ggcagcgggc ctgctgaccg gcgcaactct aggcgcgcaa gccaagaagg gcatgcgcat
     7861 cgacgtccac gcgcacttgt ggaatgacga gtatctcgat ctctgccaaa gctacggaaa
     7921 tgtagacacc ggcacgcaac gcaacaaagg cgcgggaatg ggtcaggcgg aaatggaaaa
     7981 gcgcttcgcc gccatggatg ccaacggagt cgagatgcaa attctcgaca tttgcccgca
     8041 agcgccgagt tttgaaaata aagagcacgc ggtgaaggca gcacggatgg cgaacgatct
     8101 ttacgcggat gctgtcagcc gctggccgaa aaaatttaaa gctttcgcag ctgttccgct
     8161 gcctcacgtc gacgaagcgc tcaaggaact ggaacgcgcg ctcgaccaat tgaaattcgt
     8221 aggcgcgaca atcactacgt ttatcggcaa tcgttccgtc gctgatccgg ccttcgctcc
     8281 gttttacgaa gagctgaatc gccgcgcctc ggtgttgtac attcatcctt cgggaaatgg
     8341 cgtggattct ccattcgtga ttccgtacaa cctgcgctgg gcaattggcg cgccgatgga
     8401 agacaccgtt tcgatcatgc atctgattga agcgggcatt ccgcagaaat atccaaaact
     8461 gaaaatcatc aattcgcatc tgggtggaat gattcccatg gtgttccagc ggctggataa
     8521 catttccaag tgggagcatc cgttgccgga attgccgacc gtcacggcca agcgcatgtg
     8581 gtattgcagc gtgggccacg gacatccgcc tgcgcttcgc gcggctgtcg attcgatggg
     8641 cgccgaccgc atttgcctgg gctcggattt cccgtacgaa gcaggcgagc tttacaatcg
     8701 cgccattcaa tacatcagcg actccgggtt gaaacccggc gacgcggaaa aaattctcga
     8761 tcgcaacgca gcccacgtgc tcggactcgc gtgacaacca cccaaacacc gataacgccc
     8821 gccggcaaac caaggctcac gcgcaaccag gttcgcagtt tctgggccgc atggggcggg
     8881 tggagcatgg atgggatgga ctcgttcatc tactcgctcg tactggttcc ggctctcacc
     8941 gaattgctgc cgcgctccgg tattcccgca acgcctgcga acgtcggcta ttacggcggc
     9001 atttttttta cgctattttt gattggctat ggcacggcgc tcatatgggg accaattgcg
     9061 gatcgttttg gccgtgtacg cacgatgatg ttcagcattt tgtgtttttc gcttttcacg
     9121 ctgctggctg catttgccac aggcgtgtgg agccttgcca tgtttcgctt catggcggga
     9181 gtaggaattg gcggcgagtg gtcgattggc gcgtcgctcg tttccgaaga ctggccggag
     9241 gagcggcgca ccatgggcgc ggctctgatg cacacgggct actacattgg attttttctt
     9301 gctggcattg cgaatatttt tatcggcagc cactacggct ggcgagcgat gtttgctctg
     9361 ggcggcgtgc ctgcgctgtt gattggcctc atccgcaata acgtccagga acccgcgcgc
     9421 tgggaaaaca aggtgcagga attgggcgcg aagttcacga tgcatcgcgc gtttctaaaa
     9481 cttttttctc cgctgtatcg tcgcagaacg attgtcaatt ccgtgtactt gattgtgtcg
     9541 ctcgcgggcc tgtgggctgg atcggtttac gcgccgacgg ccatgaccta catcgcgcaa
     9601 agggctggta aaaccgctgc ggaaagcgcg cgcctcgctt cctactcgac ggcgctgctc
     9661 ggcgtcgcta cagttctcgg cgcgctgatc gtgcccttta tcgcggactg gctcggccga
     9721 cgtgcgacgc tcgccatttt ttatgtggtg atgatgttcg cgatctggct ggctttcgcg
     9781 cacgtgtttt acatgcagca gaatgccatc gcgtggtttc tcgtttgctc tgcgctccta
     9841 ggtttgggag gagcgaattt tatcgtttat tccttctggc tgcctgaaca gtacggcacg
     9901 gaatgtcgcg ccagtgcttt tgcgtttatc accaatctcg gacgcttctt cgccgcaggg
     9961 tttacgtttc tggttggagc aggaatccgc cactttcaga catttgggat tcccgtagcg
    10021 atgatggccc tggcttttgt agtgggatta gcgctggtac ccttcggtga agaaaccaaa
    10081 ggtaaacgat tacccgcctg aaaatattgt ccccgctcct aaaacgtgaa ttgtgacgtg
    10141 cctttacgtg acataggctt cagagtctgt ggaatttagt ccttgcaagt cctaaattcc
    10201 acaagcgaag cctatgtcgc gaagggcttc tattgaaggg tcttgagggc ttctacaatt
    10261 tcagcggcgt ggcccttgga tttggcggtg gaccaaattt tctctatttt cccatcgggg
    10321 ccaacgagca ccgtactacg cacgattccc aaaaatgatt tccccagaaa acttttcatg
    10381 cgccgcgcgt catatgcctc gatcaccgtg aagttcggat cgctgagcag cgggaaattc
    10441 agcctttgct tggccttgaa ttttgccagt gccgctacgg catccgcgct acagcccaga
    10501 atgatggcgt ttagcttatc gaaatctttt tttagatcgc gaaattcgat cgcttcggtc
    10561 gtgcagccgg gcgtgctcgc cttgggataa aaatagagcg ccacatgctt gccgcgcaaa
    10621 tctttcaacg caatttcgct gccgtcatcg gcgggaagcc ggaagtccgg agccggatcg
    10681 ctctctttta gcatgagact gctccttcca aactttgctg tggcaggggc acggttcatc
    10741 gagaacgctc tccgtgaccg gcattgccgc tcccctacaa ctgctccgca tccagcgcct
    10801 ttcgcaggcg attcagcctt caggaaagca gacgcttgag tgtgtcgtta acggtttgcg
    10861 gattggcttg gccgcgcgaa acgcgcatca cctggccgac aaaaaattta aatacacctt
    10921 catttccagc gcgatacttc gccagattgt cgggattctt ctcaatcact tcgcggcacc
    10981 agcgttcaat ctcgcctgtg tcgctgacct gcgcgaaacc ttcggccaca atgatttccg
    11041 ccgctgtctt cccggttgca aacattcgcg cgaaaacttt ttttcctgtc gccgcgttga
    11101 tttcgccact ttccactttc gcgatcagtg ctgcaagttc cgcgggttgc actgggcttt
    11161 ccgtaatttc tttttccgct tctttcagtt gccgcagcaa ttccacttga atccagttcg
    11221 ccgcggactt cgccggggcc ccagctcgag ccacgccttc aaaataatcc gcaagctcgc
    11281 gtgtcgaggc gagcactccg gcatcgtacg gcgtgatgcc atattcccgc acgaaacgct
    11341 ggcgtttcgc ttccggcaac tccggcatgg tgctggcgat ggcatcgcgc cacgcagcgc
    11401 tgacccgcaa gggcaggaga tcaggctcgg ggaaataacg gtagtcgtgc gcgaattcct
    11461 tcgagcgcat ggattcggtg cgtccggcag ccacattcca cagccgcgtt tcctgaacga
    11521 tttttccgcc cgcttcgatc acaccgattt gccgctcaat ttcatattcc aatgcatgct
    11581 gcaagtggcg gaaagagttg agattcttca cttcggcctt ggtgccaaat ttttcagcgc
    11641 cgcgccgccg cacactcacg ttggcgtcgc agcgcagaga gccttcttcc atgttgcagt
    11701 cactcacttc cgtgtacagc atcacttctt taagggcggt caggtaggcg tgagcctcgg
    11761 tcggcgtgcg caggtccggc tctgaaacaa tttcggcgag gggtgtaccg ccgcggttgt
    11821 aatccacata acttttttcg gacgagtctg gaaatccttc gtgcagattt tttgcggcgt
    11881 cgtcttccag atgcaatcga gtgatgccaa tccgcttgat ctgaccgtcg tgttcgatct
    11941 ccagataacc gtccgcggcc agcggcaatt cgtacatcga aatctggtag cccttaggca
    12001 ggtcaggata gaaataattt tttcgagcga aacgcgaaaa atcctgcacg ttcaggtgca
    12061 gcgccagcgc cgcgcggatg ccgagttcca ccgcgtgctt atttaacacg ggcagcgctc
    12121 ccggcaagcc caggcaaacg gggcaggtgt tcgaattcgg cggatcgccg aatcgcgtgg
    12181 agcaggcgca gaacgccttg gtcaatgttt tgagttgcac gtggacttcg agaccgatga
    12241 ccggctcgta agccgccgtg atttcgggcg agatgctcgt gagtccttcc tgtgaaatct
    12301 tcgccatagg agttgcctag tataccattc gtcgcggcca atgcgtttgt tgcgcgtgta
    12361 gcgcccgcct tcaggcgggc atccggttgg tccgaaatgc ccgcctgaag gcgggcgcta
    12421 caaccggagc ctcccacctc tactgttgcc cctcaggaca ctttgggagc ctccctgaga
    12481 gacttttcca cgcaacccgt gttgaataga gtgggattaa accagtcccc gtaccggcca
    12541 tttgcccaga catattgcgg gctggtatgc gcaggtcagg aaagcccacg gaggaggggc
    12601 tacttatgcg aaagttgttg aacccccgaa gttttctgtt tttgttgctt ctcttgatca
    12661 ttccttcagc ctcgtttgcg caggtggcca tctcggtggc tatcgggcca ccggcgttgc
    12721 cggtctacgc gcaaccacct tgcccgacag agggttatat gtggactccc ggttattggg
    12781 gctggcgggc tgagggttac tactgggttc cgggcgtgtg ggttgcgccg ccgcgcgtgg
    12841 gcgtattttg gacgccgggc tactggggcc ttgctggcgg cctgtatggc tggcacgccg
    12901 gttactgggg tccacatgtt ggattctatg gtggcatcaa ttacggtttt ggatacgggg
    12961 gcgtcggatt ctggggaggg cgctgggctg gcgaacactt cgcgtataac acggccgtta
    13021 tgaacgtaaa cacgaccgta atccacaaca cgtacgtcga tcgaacgtat gtcaacaata
    13081 cgaacgtcgt gaacaaccac accagcttta acggcgaagg cggagtgaac gcccggccga
    13141 cagcaacgga acaagccgcc gaacatgaaa atcactttca gccgacatcc aaccagcttt
    13201 cccacgagca gaccgctcgc ggggacaaca atcagctggc ttcggtcaac cacggccgac
    13261 ctggaaacgc ggcgatggac tcagtaaatg gccgccgcta caaccagcag ggacgcattg
    13321 ccaacggcgt ttcctctgga cagctgacag ccggcgaaac gaaaaatctg gaaggccgcg
    13381 aagccgggtt gaacaaagaa attcataatg accgctcggc aaatggcggc acgctgacgc
    13441 cgcgagagcg gcaacaggtc aacgggcagc agaataatct gagcaagtcg atctacaacg
    13501 ataaacacaa tgcaaataca gctaactacg gcaacaacga agttggcgcg cgccgcgaca
    13561 tgcagcagca acgcgttgcc aacggcattc gcagcgggca aatgtcgccg tcggaagccg
    13621 caaaaacaga aggacgcgaa caaaatatca atcgttcagt cgcggcggat cgccaggcca
    13681 atggcggaaa gctgacgcca ggtgaaaagc aaaacattaa caggcggcag aatggaacca
    13741 gccgccagat ttataacgag aaacacaacg aaaagactgc accgaggtaa ggcggtaagt
    13801 tgggaagaat taactaaaat gcgcgttggc ggatcgtaga tccgccaacg cgcatttttt
    13861 tgcgcaaatc attccggaga tgtataggag ttcccattca atcgccctgt aatgacatct
    13921 accctcgcga cgtagaatgc cgagaaaatc caggtgagat tccaaccgaa ggatggggaa
    13981 ttgtatgcct ctcacctgtg gatcgttcga atctccgaaa ttctttgaga tgtgccgaag
    14041 tcccggaggg acgccagaaa gtagcacgcc gttttca
//
LOCUS       DASZMB010000107         8842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_387831, whole genome shotgun sequence.
ACCESSION   DASZMB010000107 DASZMB010000000
VERSION     DASZMB010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8842
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387831"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3063)
                     /locus_tag="VG272_04900"
     CDS             complement(<1..3063)
                     /locus_tag="VG272_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HWF13054.1"
                     /translation="MDFLDPRCRFCGYLGHAIRNLFLGLLAVGGAGAVHGANVPHFQR
                     PVFFERNDGQLDSSVLYTGRGLGYTVYLTRSGATMVAPAPHQASTAGQRDGGYFRLTF
                     TGTNANASVTGVGELPGRSNYFGGSDPKSWHTNISQFAKVRYTNLYPGIDVVFYVRNG
                     QLEYDILGAPKANLRAVHFSVEGVTAASTKEGDLVVPFGSGIFTLKRPRVYARNLYAK
                     PASANYLVRGKSVSFQLGPYDHNQPLVVDPALIFSTDLNSNCLLSSQPGDNCADTVSD
                     IAVDASGVYVAGSTSGPSFPSSNPTPQTTGGNSQNFQTFVIKLDPTASTLIYAAYLSS
                     SKGFAIAVDVAGAAYISGTAQVPAPSGAMSFPLTTGVFSGTVPAGLTSTSVPFAAKLS
                     ATGSAVQYSTLLQQPNTGPNPPAYVQVITPSKIAVDSQGALYVTGTAQLNNMLSAPAQ
                     WSPVPVIPGAFQQTPGTLFAMKLNPGASGLAYGTYIDGTNANNHGTGNGLAANGIALD
                     SSGDAFITGVSGGGFPITPGSYQSSDLASSGANAFIFALNPAGSAPLYSTYFGGAGQT
                     TAYGIAVDATGEATITGKSADTIPITPGAFCPLPTPPPGVQPGFIAKLNASGSALVYS
                     TSLCGYMAEGDSVALDSAGDAYVLGQTSYPGAFPLQSPIQNYYSNPVTNPTSLPQAVV
                     ALKVNPTGALLWSTFLGSGPGCPTCRIQVDSSGSAFALIDSIGNFPTTANTVGLRSPG
                     TSVNQGPQQLIKIAASLGSPVPGVIPLSVSFAPQNLGVASAAADVQVGNFGDAQITPS
                     ASITGDFSETDNCGTGVPAGSKCDVNVVFTPTAAGTRTGVLTLSFGGGIPSQTVSLAG
                     VSTAPAAGLAPTSLSFGNQATGTTSATQQVTVTNTGTGPLLIVSIQASSEFAATSTCG
                     GSIAPSATCTIQVSFTPTASGSQTGTLAINDNVPGSPQTVPLTGNQPANFVLAIGSGG
                     AASPSVTVVLGQSATYNLSIQGVNGFSGSVALACTGAPANSTCSVQPNP"
     gene            3399..4073
                     /locus_tag="VG272_04905"
     CDS             3399..4073
                     /locus_tag="VG272_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HWF13055.1"
                     /translation="MAKTHYPVDADLVMQELSRVDTHLARIILKVGSFPTKKQKPKTP
                     FESLLRAIVYQQLAGAAAATIFGRVKALGTDGICPTPEEILRATKPKLRRAGLSRQKI
                     AAVKDLAKKTIDGTVPPLAKIRRMTEEEIIERLTQVHGIGEWSVHMFLMFQLGRPDVL
                     PIKDYGILKGFQHLYGLADTPKPKAIIEHAERWRPYRSIACWYLWRAVEERNTDKVAA
                     KKKKKI"
     gene            complement(4456..5166)
                     /locus_tag="VG272_04910"
     CDS             complement(4456..5166)
                     /locus_tag="VG272_04910"
                     /inference="COORDINATES: protein motif:HMM:TIGR04305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CADD family putative folate metabolism protein"
                     /protein_id="HWF13056.1"
                     /translation="MEVSEFWQKAEQEIAKYDLLKHPFYQAWTAGELTKEDLKFYAEN
                     YYHQVSEFPTYLSALHSRMPEGQMRRDVLANAYDEECAGVAHSDLWLRFAEGMGSKGG
                     SHDENARVPEINHLVNAFRSLAKDAPVAAAFGALFAYESQVPKIAAEKRNGLKQHYGA
                     DDHACSYFTLHEKADIHHSNVWRKIISTLVEQDDRHAAEALDGMSRAAEALWTALDGI
                     ERERPNRKFASCAQPMMN"
     gene            complement(5300..5764)
                     /locus_tag="VG272_04915"
     CDS             complement(5300..5764)
                     /locus_tag="VG272_04915"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HWF13057.1"
                     /translation="MTATLRFFQFLSLGCWLGSIIFFVVFTQGIFSVMPTKDLAGLVV
                     GYSLARLHIMGIVAGCIYLLSTAVLEKSVAALVRPAALLVFVMILCTMASQYGVIARM
                     DVLRAQMGSIDATAADNPLRMTFDRLHQYSVRLESIVLFSGLAALFLTARRK"
     gene            complement(6040..6115)
                     /locus_tag="VG272_04920"
     tRNA            complement(6040..6115)
                     /locus_tag="VG272_04920"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6080..6082),aa:Val,seq:cac)
     gene            6164..7114
                     /locus_tag="VG272_04925"
     CDS             6164..7114
                     /locus_tag="VG272_04925"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWF13058.1"
                     /translation="MTTMTANLISQLQANIARVIRGKDEVIRLSLVALLAKGHLLIED
                     VPGVGKTTLAQAIANSFHCSFQRIQFTSDLLPSDVVGTSVFNQIEQRFDFKPGPIFAN
                     IILADEINRTTPKTQSALLEAMNEAQVTVDNHTYKLPHPFMVLATQNPVEHHGTYPLP
                     ESQLDRFILRIGMGYPSRDSEREIMQQHASGRNAAAEITPVMEAEEVEVMQHAVEKVR
                     VDDSLRDYALDIVERTRTSEQLTLGVSPRGLLMLQRAAQARAFLEARDFCTPDDFKQL
                     VIPVFAHRVVVNSRYAATQKKSTQSEMVLREIMETTPVPL"
     gene            7133..8539
                     /locus_tag="VG272_04930"
     CDS             7133..8539
                     /locus_tag="VG272_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWF13059.1"
                     /translation="MSAEFVERRVGSPLLRKVVGVSRAAQWARLRETLDRPSWRSFGI
                     AMGALAGALFLALYSGAAAEEGHLFIAGGSALAALVLAGWVALTVVPALARRTPLGWL
                     SYQIEYKVTREGIVYIAGILVVALAALNTGNNLLFMVLACLMAGLLISGLLSRVVLGG
                     IDVNLELPEHIFARRTILAVAQLYNEKQMTPSFSVSLVSETAPQASKNPLTNSLPQIL
                     DRPVYFPYIPHRQTVRQSVELTFPRRGIYRQDALGLRTKFPFGFLQKTRRANSKIEVV
                     VYPQIQPTEEFYEVLPLVSGELESYQRGRGNDLYAIREYQSNDSARHVDWKASAKIGA
                     LQVKEFAREDERRVMLVLDPYVDPQATDAKAAEQFERAVTLCAGLAWHFYEINSVMEF
                     RSAGFGTPRMSAGEVIYDILRYLASVTPLRQEAGKSFLDTLGDTHDIFKIVLTNQPSG
                     SIPSSLWNTAYFLFVSSL"
BASE COUNT         2000 a         2434 c         2489 g         1919 t
ORIGIN      
        1 cggattcggc tgcacggaac acgttgaatt ggcgggcgca cccgtgcagg caagcgccac
       61 cgatccgctg aatccgttga ctccctgaat gctgagattg taggtagcgg attgtccaag
      121 cacgactgtg accgagggcg aggcagcgcc gccgctgcct attgcgagca cgaaattcgc
      181 gggctggttc ccagtaagcg gtaccgtctg cggactaccg ggcacgttat cgttgattgc
      241 caacgttccg gtttgagaac cagaggcggt tggagtaaac gacacctgga tcgtgcaagt
      301 tgcgctcggg gcgatcgaac cgccgcaggt gctcgttgcg gcgaattcac tcgacgcctg
      361 aatcgagacg atcagtaatg gtcctgttcc tgtgttggtg accgtcactt gctgtgtcgc
      421 gctcgtcgtc ccggttgctt ggttcccgaa ggacaatgaa gtaggagcga ggccggccgc
      481 tggcgctgtg ctgacaccgg cgagcgacac cgtttgcgat ggaatgcctc caccgaaact
      541 cagtgtcagg acgccggttc gcgtccccgc agcggtcggt gtgaacacca cgttgacgtc
      601 gcacttgctc ccggctggaa ctccggtgcc gcaattatcg gtttccgaga aatcacccgt
      661 gatggaagcc gagggcgtta tctgcgcatc gccaaagttg ccgacctgca catctgcagc
      721 ggcgctcgct acgccaagat tttgcggcgc aaaactcacc gacagcggga tcacgccggg
      781 cacgggcgaa cccaaactcg cggcaatctt gatcagttgc tgcggccctt gattcacgct
      841 ggttccaggg cttcgcaggc cgacggtgtt cgcagtggtg gggaagtttc cgatcgagtc
      901 gatcaaagcg aaggcgctgc ctgaagagtc cacctgaatc ctgcaggtcg ggcacccggg
      961 gccgctgccc aaaaatgtcg accagaggag cgcccccgtc ggattgacct tcagtgcgac
     1021 gactgcctgg ggcaaactgg tagggttcgt tacgggattc gagtagtagt tctggatcgg
     1081 gctctgtaga ggaaatgcgc cggggtaaga agtctgtccc agaacatacg catcgcccgc
     1141 ggaatcgagc gccacgctat cgccttcggc catgtatccg caaagcgaag tcgaatagac
     1201 gagcgcggaa ccgctcgcgt tcagcttggc gataaagccc ggttggacac ctggcggagg
     1261 cgtgggaagc gggcagaatg cgcccggtgt gatcggaatt gtgtccgcac tcttaccggt
     1321 aatggtagct tcgccagtcg cgtcgaccgc gatgccatat gccgttgttt ggcctgcccc
     1381 gccaaaatac gtcgagtaaa gtggagcact tcccgccggg ttcagtgcaa atatgaaagc
     1441 attagcgccc gatgatgcca gatccgatga ttggtacgaa ccgggcgtta tcgggaaccc
     1501 gccccccgat acgccggtta taaacgcgtc gccgctggaa tcgagtgcga tcccgttggc
     1561 cgcaaggccg ttgccggtgc catggttgtt tgcattcgtc ccgtcgatat acgtgccgta
     1621 cgcgaggccc gaagcgcccg gattcagttt catcgcgaac aatgtgcccg gggtctgctg
     1681 aaacgctccg ggaatgaccg gaacgggcga ccactgcgcg ggggcgctca gcatgttatt
     1741 cagttgcgca gtccccgtga cgtagagcgc gccttgcgag tcgacggcaa tcttcgacgg
     1801 agtaatgact tggacgtagg ccggaggatt cggacccgta ttcggttgct gcagcagggt
     1861 ggagtattgg acggcggaac ccgtggcact cagcttcgca gcgaaaggta cggaagtgga
     1921 cgtgagacct gcgggcacgg tgccgctgaa tacgccggtc gtcaacggaa atgacatcgc
     1981 tccactcggc gctggaacct gcgcggttcc ggaaatatag gcggcaccgg ctacgtcgac
     2041 ggcaatggcg aagcctttgc tgctgctcag ataagccgcg tagatcaggg ttgaggccgt
     2101 cgggtcgagc ttgatcacga aagtctgaaa gttttgggaa tttcctccgg tggtttgagg
     2161 agtcggattt gatgagggga aacttggtcc gctcgttgag cccgctacgt acacgcccga
     2221 ggcgtcgacc gcgatgtcgc tcaccgtatc ggcgcaattg tcgcccggct ggctggacag
     2281 aaggcagttg ctgttgagat cggtggagaa aatcagcgcg ggatccacta caaggggctg
     2341 gttgtggtcg tagggtccga gctggaaact cacgctcttt ccgcgaacga ggtagtttgc
     2401 cgatgcgggc ttcgcgtaga gattcctcgc gtagacccga ggtcttttca acgtgaagat
     2461 cccgctgccg aaaggaacga ccaggtcccc ttctttggtc gaggctgctg tgactccctc
     2521 gaccgagaaa tgcaccgctc tcaaattcgc ttttggcgcc cccaggatgt catattccaa
     2581 ctggccatta cgcacataga aaacgacgtc gatccccgga tacaggtttg tgtagcgcac
     2641 tttcgcgaac tgcgaaatat tggtatgcca tgactttgga tcggacccgc cgaaatagtt
     2701 gctccgaccc ggaagttccc cgactccggt cacacttgca ttagcgttcg ttcccgtgaa
     2761 ggtcaagcgg aagtagccgc cgtcacgctg gccggcggtg cttgcttggt gcggcgccgg
     2821 tgcaaccatg gttgcgcctg agcgagtcaa gtataccgta tagcccaggc cgcggcccgt
     2881 gtagaggacg gacgaatcaa gctggccgtc attccgctcg aagaaaacag gtcgttgaaa
     2941 atgcggcaca ttggccccgt ggacggctcc agcaccaccg accgcaagca ggcccaaaaa
     3001 caagtttcga attgcgtggc ccaggtaccc gcaaaatcta catcgcgggt cgaggaaatc
     3061 cattagacac cccgcacgag tctgcttgga tccgcactca aggaacgaaa atgctaatga
     3121 atagtccatt acattcatgg taaatgccat cgggtgtaat gtggagaaca tagggcgaat
     3181 cgataacgaa agtaggccca cctcacttta attcctgggc aattccagcc agagctgccg
     3241 gggatggcac gaaactccgc gcttagtttc aacgaagaaa agaagacagg gcctcaaact
     3301 agctcgctcg gtacctgata aaccgggcgt tctcggaatc tggtttgcac ccaactcttg
     3361 acgcgatctc gactcgctcc tatgctccat attctgttat ggcaaaaaca cattatccgg
     3421 ttgacgctga tctagtgatg caggaacttt cgcgtgtaga cacgcatctc gcgcgaatca
     3481 ttctcaaggt cggctcgttt cccacgaaaa aacaaaaacc gaaaactcca tttgaaagcc
     3541 tgctgcgggc catcgtttat cagcagctcg cgggcgcggc ggctgcaacc attttcggac
     3601 gcgtcaaagc gctgggaacg gatggcatat gccccacgcc ggaagaaatt ctgcgcgcga
     3661 caaaaccgaa attgcgtcgc gcgggattgt cgcggcaaaa aattgccgcg gtaaaggatc
     3721 tggcgaaaaa aacaatcgac ggaaccgtgc ctccgctggc caagattcgg cgcatgaccg
     3781 aggaagaaat catcgagcgg ttgacgcagg tacacggaat cggcgaatgg tccgtgcata
     3841 tgtttctgat gtttcagttg gggcggcccg acgtcctgcc gattaaagat tatggaattc
     3901 tgaaagggtt tcagcatctc tacgggcttg cggatacccc aaagccaaag gcgatcattg
     3961 aacatgcgga gcggtggagg ccttaccgct cgattgcatg ctggtatttg tggcgcgcag
     4021 tcgaggaaag aaatacggac aaagttgccg cgaagaaaaa gaagaagatt taaatgtagc
     4081 gacaggcttc agccggtcag cctttgattt ctgcagcgtc agcaaaattc aaaagcagac
     4141 aggcgaaaga gtctgcgcga gaactgcaga atcgttgtgg gtcgtggctt tagccacgac
     4201 ataagtcctg caaaatgcgt gcggctttac agtttgcgga aaaagtagtt tattctgtca
     4261 ttccgagcga agcgaggaat ctctcttcga tttaagtata aggaaataaa gaaggaggga
     4321 ttcctcgctt cgctcggaat gacaaaatgt tgagtttttc cgcagcctct ttagccgctg
     4381 aagcttcaat tttgcagttc tcacacagat tctaaagcct gccccctaca caaaacacca
     4441 aacccaaccc aaaaactaat tcatcattgg ttgcgcgcag cttgcgaatt tgcgattggg
     4501 gcgttcgcgc tcgataccgt cgagggccgt ccacagcgct tccgccgcgc gagacattcc
     4561 atccagagct tccgccgcgt gacgatcatc ctgttccact aacgtcgaaa taatttttcg
     4621 ccacacgttg gaatggtgaa tatccgcttt ttcatgcaac gtgaaatagc tgcacgcgtg
     4681 gtcatcggct ccgtaatgct gcttcagtcc gttgcgcttt tcagcggcaa tttttgggac
     4741 ttgcgactcg taagcgaaga gcgctccaaa tgcggcagcc accggcgcat cttttgccaa
     4801 tgagcggaat gcattcacga gatgatttat ttcggggact cgcgcatttt catcgtgact
     4861 gccgcccttg cttcccatgc cttcagcaaa ccgcagccac aaatcggagt gcgccactcc
     4921 tgcacattct tcgtcgtagg cattggcgag cacatcgcgg cgcatctggc cttccggcat
     4981 ccgggaatga agcgcagaca ggtacgtggg aaattcggag acctggtggt aataattctc
     5041 ggcgtaaaat ttcaaatctt ccttcgtcaa ttcgcctgct gtccacgcct gataaaatgg
     5101 gtgcttcagc aaatcgtatt tcgcaatttc ctgctcagct ttttgccaga attcggaaac
     5161 ttccatcttg attccctccc gtgggggttg tgagttttaa ttttgttccc cgaaccgtgc
     5221 gcgagttaaa acaagcgagt taaaataaga caaacccgct cgtccaaaat tcggacggcc
     5281 tctacaaaat cgtacggcgc tattttctgc gcgccgtcag aaacaatgct gccagacctg
     5341 agaataacac gatgctttcg aggcgcacgg aatattgatg caaacggtcg aaggtcatgc
     5401 gcagggggtt atctgccgcg gtcgcatcga tcgagcccat ctgcgcacgc agcacgtcca
     5461 tgcgcgcgat cacgccgtac tgcgaggcca tcgtgcacag gatcatcaca aacactaaga
     5521 gcgcggccgg gcgcacaagc gccgcgacgg atttttccaa aacggccgtg gagagcaagt
     5581 agatgcatcc ggctacgatg cccatgatat gcaatcgcgc gagcgaataa cccacaacca
     5641 gtccagccag atctttggtg ggcatcacgg aaaaaatgcc ctgcgtgaaa acgacgaaaa
     5701 agataatgct gcccagccaa cagccaagcg agaggaattg aaagaatcgc agtgtcgccg
     5761 tcacttttct tgttccttct tgaggttcga gcagttacgc caaccccgga tcaccaaact
     5821 gcacggctat tccagaccgt cgccagagta ccacagcgga agcgcagctt gttcacactg
     5881 cgagtctcag ttttccatcg agctcaaaaa ccaatctgct tagccctttg catcacagtc
     5941 cattgctcgc ggtgcgctat caaaactttt taggcacagc aggaatggca agtattaaag
     6001 atttctagga aacaatggca acataggagt gctggagaat ggtgggcgat caaggacttg
     6061 aaccttggac ctctcgcgtg tgaagcgagc gctctaacca ctgagctaat cgcccggaaa
     6121 tcaagcttcg tttgtagcag aagcgatagg gattttcaac ccgatgacca ccatgacggc
     6181 aaatcttatc tcgcagttgc aggcaaacat tgcacgcgtg attcgcggca aggacgaagt
     6241 gattcgtctc tccctggtgg ccctgctggc caaaggccac ttgttgattg aggacgtgcc
     6301 cggagtgggc aagacaactc tcgcgcaggc gatcgccaat tccttccact gctcgttcca
     6361 gagaattcag ttcacctctg acctgctccc gtccgatgtg gtcggtacca gcgtgttcaa
     6421 ccagattgag cagcgtttcg atttcaagcc cggcccgatt ttcgccaata ttattctggc
     6481 ggatgaaatt aaccgcacca cacccaagac gcaatccgcg ctgctcgaag ccatgaacga
     6541 agcgcaagtg accgtcgaca accacaccta caaattgccg cacccattca tggtgctggc
     6601 aacgcagaat cccgtggaac atcatggaac gtatccgctg ccggaatcgc agctggaccg
     6661 gtttattttg cgcattggta tgggatatcc ctcgcgtgac agcgagcgcg aaatcatgca
     6721 gcagcatgcc agcgggcgca acgcggcggc ggaaatcact cctgtgatgg aagctgagga
     6781 agtggaagtc atgcagcacg ctgttgagaa agtgcgcgtg gatgacagcc tccgtgacta
     6841 cgccctggac atcgtcgaac gcacgcgaac ttccgagcag ctcacgctcg gcgttagtcc
     6901 acgcggtttg ctgatgctgc agcgcgcggc gcaggcgcga gcgttcctcg aagcgcgcga
     6961 tttctgcacg cccgatgatt tcaagcagtt ggttattcca gtcttcgccc accgcgtggt
     7021 agtcaactca cgctacgcgg caacgcagaa aaaatcgacg caatctgaaa tggttttgcg
     7081 cgaaatcatg gaaaccacgc ctgttcctct ctgaatccgg tgagtattta agatgagcgc
     7141 tgaatttgta gaacgccgcg ttggctctcc acttttgcgc aaagtggtgg gagtgtcgcg
     7201 tgcagcccag tgggcacgcc tgcgcgaaac gctggatcgt cccagttggc gcagctttgg
     7261 tatcgccatg ggcgcgctgg ccggagcact ttttctagcg ctgtattccg gagccgccgc
     7321 cgaagaaggc cacctgttta ttgccggagg ctcggcactc gcggctctgg tgcttgccgg
     7381 atgggttgct ctgacggttg ttccggcgct agcaaggcgc acgccgctcg gctggctcag
     7441 ctatcagatc gaatataagg tgacgcggga aggcatcgtg tatatcgccg gcattctggt
     7501 ggtggcgctc gcggcgctca acacgggtaa caatctgctg ttcatggttc tggcgtgttt
     7561 gatggccggg ctgctgattt ccggattgct atcgcgcgtg gtgctgggcg ggatcgatgt
     7621 gaacctggaa ttacccgagc acattttcgc gcggcgcacg attctggccg tcgcccaact
     7681 ttacaatgaa aaacaaatga cgccgtcttt ttccgtttcg cttgttagcg aaaccgcgcc
     7741 gcaggccagc aaaaatcccc tgacaaattc gctgccgcaa attctcgatc ggccggttta
     7801 ttttccgtac attccccacc gtcaaacggt gcggcagagc gtggagctta cgtttccgcg
     7861 gcgcggaatt tatcgccagg acgccctggg attgcgcacc aaatttccct ttggattcct
     7921 gcaaaaaacg cggcgcgcga attccaagat cgaagtcgtc gtctatccgc agattcagcc
     7981 caccgaggaa ttttatgaag tgctgccgct cgtcagcggc gaactggaaa gttaccagcg
     8041 cgggcgcgga aacgatttat atgcgattcg cgagtaccag tcgaatgaca gcgctcgaca
     8101 cgtcgactgg aaagcttccg ccaaaatcgg cgccctgcag gtaaaagaat ttgcgcggga
     8161 agacgagcgc cgcgtgatgc tcgtcctcga tccttacgtg gacccgcaag cgacggacgc
     8221 caaggctgcc gagcaatttg agcgcgccgt aaccctttgc gccggcctcg cctggcattt
     8281 ctacgaaatt aattccgtga tggagtttcg ctccgccggc tttggcactc cgcgcatgag
     8341 cgcaggcgaa gtgatttacg atatcctgcg ttatctcgcg agcgtaactc ccttgaggca
     8401 agaggccggc aagagttttc tcgacacgct cggcgatacg catgacattt ttaaaattgt
     8461 gctgaccaac cagccaagcg gctcgattcc ctcgtctctc tggaacaccg cctattttct
     8521 gttcgtttca tccctctaat tttaaatctg gaaaataaat tcaagtgggt gggcattaac
     8581 tggcataggc ttcagcctgt ggggtttagt cctcgctaga cttactctgc aaaagtgtta
     8641 gttcttgcat gcactaaacc ctacaggctg aagagtctgt gtgagaactg caaaattgga
     8701 gcttcagcgg ctaaagaggc tgcggaaaaa ctcaacattt tgtcattccg agcgaagcga
     8761 ggaatccctc cttctttatt ttcttatact taaatcgaag agagattcct cgcttcgctc
     8821 ggaatgacag aataaactac tt
//
LOCUS       DASZMB010000108         7591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4494352, whole genome shotgun sequence.
ACCESSION   DASZMB010000108 DASZMB010000000
VERSION     DASZMB010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7591
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4494352"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /gene="rnpB"
                     /locus_tag="VG272_04935"
     ncRNA           complement(<1..537)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VG272_04935"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(804..1520)
                     /locus_tag="VG272_04940"
     CDS             complement(804..1520)
                     /locus_tag="VG272_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWF13060.1"
                     /translation="MSKFTLSATITLAVGTMFIAGCATKNYVKKSITPVDQKVDQVDQ
                     ASQKRDSSQVADINKTNTALDETDKKLSATDEIAKTADGTAKGAMAKGNQNSKEINDL
                     RGVIANIDDYKPAGDATVVHFGINKDTLTKDEKAKLDAMATQLGSQQRYFITVEGFTD
                     QTGPASLNDQLSRARANQVISYLVGSHDVPVYRIHMVGLGSQKLVDEGKGRKAREASR
                     RVEVTLFTAKPLTMSAAGGN"
     gene            complement(1775..3877)
                     /gene="ligA"
                     /locus_tag="VG272_04945"
     CDS             complement(1775..3877)
                     /gene="ligA"
                     /locus_tag="VG272_04945"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HWF13061.1"
                     /translation="MAKGATINKKIEKLREELRRHEYLYYVQDEPEISDIKFDRMLAE
                     LKQLEAEHPELVTPDSPTQRIGGAPRKGFETRRHSPPMMSLDNTYSTEELDDFDRRVR
                     EISGREGIDYVTELKFDGLSISLVYEGGVLVRGVTRGDGTTGEEVTANIRTIRSIPLS
                     VDTEVLKKLNMPLDFEVRGEIIMPLRAFEALNEKQVEDGGKRFANPRNAAAGAVRMLD
                     PEITRSRRLDFYGYFLLANGRMPTRLHSEALEALHKLHFKVSADWRVCHSLEAVKKFI
                     DSWDGRRDKLPYEIDGIVIKVNDIGQQQELGFTSRAPRWAVAYKYPARQETTVVNDVL
                     FQVGRTGTLTPVAMLEPVQIGGVIVSRSTLHNMDEIGRLGLHIGDSVIIERAGEVIPH
                     IIKVVKEGKTRRTVVVPEHCPECHSRIHKDPDEVAYRCVNASCPAKRRESLLHYAGRH
                     AMNIDGLGEKIVDQLVDKGMVKDFADLYELDLKEVAKLDRMAEKSAQNLLEEITASKK
                     NSLARLIYAMGMRFVGERTGQLLAEHFSSLQKLADASVEQLEEVPEVGPKVARSIADF
                     FSEAANRKIIQRLEKEGLNMTEERVAPEDTRFAGMTFVFTGTLARRSRDESGAEVIRH
                     GGKITNSVSKLTSYVVVGADPGSKFEKAKSLGVAILNEDEFEDLLKGKRAVAVARSKA
                     QSGTEESKKKARQRKSSK"
     gene            4132..5094
                     /locus_tag="VG272_04950"
     CDS             4132..5094
                     /locus_tag="VG272_04950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HWF13062.1"
                     /translation="MSVPRALADSSGANHVPLNIRDAVLIVNPAAGGGKRVKQLEEAR
                     RIFRNAGIETELQFTTAPGDATPMARRAVDASRELVIVCGGDGTVNEVVNGLACSRVP
                     LAVLPAGTANVLAKELALPWNLPRAAKRIINAPLRRIALGLAIPEKSSGEPRYFLSLA
                     GAGADGALVAAVRPEVKLKAGILAYWQEGLRQLSKYDFPMFRTSMAGNAIDIDASLVV
                     VGRTKHYGGPFKITTEAGLLQPEFELAFVTTRSPWRYLAYLPLLWAGKLRGTRYVRFF
                     KTSSLQCASNSESQVQIQVDGEPAGRLPVEFRIVPDALTLAIPD"
     gene            5244..6251
                     /locus_tag="VG272_04955"
     CDS             5244..6251
                     /locus_tag="VG272_04955"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HWF13063.1"
                     /translation="MEPVTHALTSIALGRAGLNKIARAATPMLLVSGLAADVDWVTRL
                     GGAATFLHGHRTVTHSLVGTLAIAAIVAVTAWMLGQKYPKFAVGIYAALGISAIGAGA
                     HLFLDLLNDNGVKLFWPFNAKWYAWDLADSVDSWILFFLIVGLLIPELFRLVHDEIGS
                     RPKRNGRQRGAIFALVCVGFVIAGRIVSHERAIALLDAREYRAQTPLLAAAFPKSSNP
                     FLWSGVVETDNALFNLEVPVGLGRAFDPDLADVHFKPQPSATLKSAIACATAIEFLNF
                     ARFPLASVQPEGDGFQVRLRDMRFANELPGRRGIIAVIHLNAQSQVISEYLEFDSSPS
                     Q"
     gene            complement(6306..6914)
                     /locus_tag="VG272_04960"
     CDS             complement(6306..6914)
                     /locus_tag="VG272_04960"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HWF13064.1"
                     /translation="MELPQPKFHLPHWLQAIVATAGGFGLFLIAFLDSSVLSFPIIND
                     LLLIDLSVRNAARMPYYAAMATFGSVGGCLFLYYLARKGGEAMFHKHAGPRAKHIHAW
                     INRNGFLSILVTALLPPPTPFKIFVIAAGALEMPVQKFVLGLLAARAIRYFGEGFLAI
                     RYGDQAGLFLVTHKLEVAGIVLGIILGLYLLSQLAFRPPKET"
BASE COUNT         1659 a         2173 c         1888 g         1871 t
ORIGIN      
        1 aagccggatt ttgtacaacg catgggcttt gacgccgccg cgctgtggcg atcattcctc
       61 taggctcagg ttcgcacctg agctccagca gcctacccga gggtcctgac gagccgggct
      121 ggctctcccc tcctatttgg ccttgcacca cgcggggttt gccgtgcccc cgatgttgcc
      181 atcggggcgg tgggctctta ccccaccgtt tcacccttac caggcacaat cactttctgc
      241 acctcgcttg cgcgagttgc tttcaggccg tccgaagggt ttgccttggg atggccaccg
      301 agaagcaatg caccggcggt ttattttctg tggcactgtc cgtagccggt ttctcgcttc
      361 gtttgcgcga atcgaaaaag cagccccctg gcgttaccag gcgcgtcgcc ctttagtccc
      421 ggcgcagaga gcggaaactt ccgttcccac aatgctacgc gaagaccacg gtgtccggac
      481 tttcctcccg gcctcgcttc ttgcgaagaa aggcccagcg atcacccggc ttgcccgcca
      541 acgcaagtat atcatttttg ttgccgtggt aataagcgtc atggcgggac aattccgctc
      601 aatcgatccg ggaaatcaca gaaactgaag agtgacatcc tacttgtcag agatcctttg
      661 cgttggagat ttataagttt cgacaaaaac acacgactgg tcttgcaatc aaggagggat
      721 taaaaacagt gaagatatta accgtgagtg tcagcctggg ccaacactca cggcctcaaa
      781 acaggttgat tctgcggagc gagctagttt cccccggctg cggacatggt cagcggcttg
      841 gcggtaaaca aagtgacttc gacgcggcgg ctggcttcac gtgccttgcg ccccttgccc
      901 tcgtcgacca atttctgaga tccgagccct accatgtgaa ttcggtagac tggaacatca
      961 tggctgccca ctaggtagga aatcacttgg ttggcgcgcg cacggctcag ctgatcgttt
     1021 agagaggctg gtccagtttg atccgtgaat ccttcgacgg tgatgaaata gcgctgttgg
     1081 gaaccaagct gtgtggccat ggcatccaac ttcgcctttt catccttcgt cagagtgtcc
     1141 ttgttgatgc caaagtgcac gacagtagcg tccccggctg gtttgtaatc gtcaatgttc
     1201 gcgattacgc cgcggaggtc attaatttcc ttggaattct gattgccctt ggccatcgcg
     1261 cccttagccg tgccatcagc cgtctttgcg atttcatccg tggctgatag cttcttatcg
     1321 gtctcgtcga gcgccgtatt ggtcttattt atgtctgcga cttgcgacga gtcacgcttt
     1381 tgcgaagctt gatccacctg atcaactttc tgatcaactg gagtaatact cttcttcaca
     1441 taattcttag tcgcgcaacc cgcgatgaac atcgtaccga ctgcaagtgt aattgttgcc
     1501 gacaaagtaa acttactcat tggcgatctc cccctgcaaa tctcagtttt gggccccttg
     1561 agccgtagcc ggacttctcc gttctcccaa gactcaactt aactatcgca cgtagtcaac
     1621 caatttggga ataaatttca ctgtactctt aagtctcact catacaaaga aatacggagc
     1681 ttcaaaccgt tggcagagca aatgcccctt cgccttgcta ggtaaacttt acacgatccg
     1741 agtcattcag ggtacctttc tccttatctg cctatcactt ggagcttttt ctctgacgtg
     1801 cttttttctt actctcctca gtcccacttt gcgctttgga cctcgccact gccacggctc
     1861 gttttccctt cagcaaatct tcaaattcgt cttcattcaa aattgccacg ccaagcgact
     1921 tggctttttc aaatttcgag ccaggatccg cccccactac aacgtagctc gtcagtttgc
     1981 tgaccgaatt cgtgattttt ccgccatggc gaatcacttc cgctccactc tcatcgcgcg
     2041 agcgccgcgc cagcgttccg gtaaaaacaa atgtcattcc ggcgaaccga gtatcttccg
     2101 gagccactcg ttcttccgtc atgttcagcc cttccttttc caggcgctga attatttttc
     2161 gattggcggc ttccgagaaa aaatcggcaa tgctgcgggc aaccttcggc cctacttccg
     2221 gcacttcctc taattgttcc acggacgcat cggccagttt ttgcagcgat gaaaaatgtt
     2281 ccgccagcaa ctgtcctgtg cgctcgccca cgaaacgcat ccccattgcg taaataagcc
     2341 gagcaagact gtttttcttg ctcgctgtaa tttcttccaa aagattttgc gcggattttt
     2401 ccgccatgcg atcgagcttg gcgacttcct tcagatccaa ctcataaaga tccgcaaaat
     2461 ctttcaccat tcccttatca acaagctggt ccacaatttt ttcacccagg ccgtcgatat
     2521 tcatcgcgtg ccgtcccgca tagtgaagta gcgattccct gcgcttggcc gggcagctcg
     2581 catttacgca acgataggcg acctcatccg gatccttgtg aatccggcta tggcactccg
     2641 ggcaatgctc cggcacgaca actgtccggc gagtcttccc ttctttaacc accttgataa
     2701 tgtgcggaat gacctcgccc gcgcgctcga tgattaccga atcgccgata tgcaggccaa
     2761 ggcgcccaat ttcatccata ttgtgcaaag tagatcgtga aacaatgacg ccgccaattt
     2821 gcaccggctc aagcattgcc acaggtgtta gcgtgcctgt tcggcccact tgaaatagaa
     2881 catcgttcac cactgtagtt tcctgccgcg caggatattt gtacgccacg gcccaccggg
     2941 gcgcccttga cgtgaatccc agttcctgct gctgcccgat atcgttcact ttgattacga
     3001 tgccgtcaat ttcgtacggc aatttatcgc gccggccgtc ccacgaatcg atgaattttt
     3061 tcactgcttc tagcgagtga cacacgcgcc aatcggcgga aactttgaaa tgaagcttgt
     3121 gcaacgcttc cagcgcttcc gagtgaagtc gagtgggcat tcgcccgtta gccagcaaaa
     3181 agtaaccata aaaatccagg cggcgcgatc ttgtaatttc cggatcaagc atgcgcaccg
     3241 cgcctgccgc ggcattgcgg ggattcgcga agcgctttcc gccatcttcc acctgttttt
     3301 cgttcagcgc ttcgaaggct cgcaagggca tgatgatctc gccgcgcact tcaaaatcca
     3361 gcggcatatt cagcttcttg agcacttccg tatccacgga cagcgggatc gagcgaatcg
     3421 ttcggatatt tgcagtcact tcttcgcccg tcgtgccgtc gccgcgagtg acgccccgga
     3481 ccaatacgcc gccttcatac accagcgaaa tactcaagcc gtcgaatttg agttcggtga
     3541 catagtctat gccttcgcgt ccggaaattt cgcgcacacg ccggtcaaaa tcgtcgagtt
     3601 cttcggtcga atacgtgtta tccaggctca tcatgggcgg agagtgccgg cgcgtttcga
     3661 acccttttcg cggagctccg ccaatgcgct gcgtaggtga atccggggtg accagctcag
     3721 gatgctccgc ctcgagttgt ttgagttcag ccagcatgcg gtcaaacttg atgtccgaaa
     3781 tctccggctc gtcctgtacg taatacaaat attcgtgccg ccgcagttcc tcgcgcagct
     3841 tttcgatttt tttgttgatc gtcgcgccct tagccataca acctcagaaa gtcgatagaa
     3901 tcagccgttc agtatacaat gcgccccggc cttgaaacag tccgtacctt taccggagct
     3961 gtcctaattt gaagagaatt tgcgtggcgc ggttccttag aaggtcagga cttcagtccc
     4021 gacgacgagc ttggccgagt ggaaacttgg ccctcctaaa tttaagaaat tttcaaatga
     4081 ggacattacc ccttttccgg ccaatcacgc tatcttggtt attggtaagc catgtcagtt
     4141 cctcgagctc tcgccgattc gagtggagcc aatcacgtgc ctctcaatat tcgcgacgcg
     4201 gttctcatcg tcaatcccgc agccggtggc ggcaagcggg ttaagcagtt ggaagaggct
     4261 cgccgcattt tccgcaacgc cggaatcgaa accgagcttc agttcacaac ggctcctgga
     4321 gacgccaccc ctatggcccg tcgcgccgtc gacgcatctc gcgagctcgt aatcgtttgc
     4381 ggaggcgatg gcacagtcaa cgaagttgtg aatggacttg cttgcagccg ggttccgctt
     4441 gccgtcttgc ctgccggcac cgccaatgtg ctcgccaagg aactcgcgct gccctggaac
     4501 ctgcctcgcg ccgccaagcg catcattaat gcgcctctca ggcgcattgc ccttggcttg
     4561 gccatcccgg aaaaatccag cggggagcca cgttattttt tgagcctggc aggtgccggc
     4621 gccgatggag ccctggttgc cgccgtaagg cctgaagtca agctgaaagc cggcatcctc
     4681 gcgtactggc aggagggcct ccgccagcta tcgaaatatg atttccccat gtttcgaacg
     4741 tccatggcag gcaacgcgat cgatatagac gcttcactcg ttgtcgtagg ccgaacaaaa
     4801 cattatggcg gcccgttcaa gatcacgaca gaagctggcc tcctgcaacc ggaattcgaa
     4861 ctggccttcg tcaccacgcg cagtccttgg cgctacctcg cttacctgcc gttactttgg
     4921 gcagggaaac tacgcggcac gcgatacgta cgtttcttta aaaccagttc cctgcaatgc
     4981 gcatccaaca gcgaatcgca ggtccagata caagtggatg gcgagcccgc tgggcgcctt
     5041 cctgtagagt tccgcattgt tcctgacgct ctcaccctag ccatccccga ttaggtgtag
     5101 tatctccgcg gcatcagaac gcatggtttt tgtggcatag gcttcagcct gtgggggtta
     5161 gtccttgcaa ggccaaaact ccacaggctg aagtctatgc cacaaaaacc gatcccatac
     5221 attgccgcgg atgaatacac accatggaac ctgttaccca cgcgctcacc tccatagcgt
     5281 tgggccgcgc tggcctaaat aaaatcgcgc gcgcggcaac gcccatgctc ctggtctccg
     5341 gcttagccgc ggatgtggac tgggtcacaa ggctgggcgg agcggcaacg tttctacatg
     5401 gccaccgcac tgttacgcat tcgcttgtag gtacgctggc gatcgccgcg atcgtcgcgg
     5461 ttacagcctg gatgcttggt caaaaatatc caaaattcgc ggtgggaatt tatgctgcgc
     5521 tagggattag cgcaatcggc gccggagcac atttattttt ggatttgctg aacgacaatg
     5581 gcgtgaagtt gttctggcct ttcaacgcaa aatggtacgc atgggattta gcagattcag
     5641 tggactcatg gattcttttc ttcctgatcg tcggtctgct gattcccgaa ttattccgac
     5701 tggtacatga tgaaattggt tcgaggccaa agcgcaatgg gcgtcaacgc ggcgcgatat
     5761 tcgctttggt gtgtgtcgga ttcgtgatcg caggacgaat agtttcgcac gagcgtgcca
     5821 ttgcgctgct cgatgcgcgc gagtatcgag cgcaaacgcc gcttctagcc gcggcgtttc
     5881 caaaatcttc aaatccattt ctctggtccg gggttgtgga gacggataac gcgcttttca
     5941 atctggaagt gccagtgggg ttaggacgcg cgttcgatcc cgatctggcg gacgtgcatt
     6001 ttaaaccgca accttccgcc acactgaaaa gcgcgatcgc atgcgccacc gccatcgagt
     6061 tcctgaattt tgcgcgattc ccactcgcga gcgtccaacc cgaaggcgac ggttttcagg
     6121 ttcgcctacg cgatatgcgc tttgcgaacg aacttcccgg ccgccgcgga atcatcgccg
     6181 taattcacct gaacgcgcag agtcaggtaa tcagcgagta cctggaattt gacagctcgc
     6241 cgagccaata aattattaat cttcgcagga cgcaggcgcc acgaaaatcg acctctcacg
     6301 gagatttaag tttctttcgg tgggcggaat gcgagttggg acaacaggta aaggccaagg
     6361 ataatgccca gcacaatccc tgcgacttcc agtttatgcg tcaccaaaaa taatccggcc
     6421 tgatcaccat atcgaatggc caaaaaacct tcgccaaagt atcgaatggc gcgcgcggca
     6481 agcaaaccca gaacaaattt ctgcaccggc atttccagtg cgcccgccgc aatcacaaat
     6541 attttaaacg gagtaggagg tggcagtagc gcggtaacca gaatgctgag aaacccgttg
     6601 cggttgatcc atgcgtgaat atgcttcgcc cgcggcccgg cgtgcttgtg aaacatggcc
     6661 tccccgccct tgcgcgcaag gtagtaaaga aagaggcacc ctccgaccga cccaaaagtt
     6721 gccatcgccg cgtaatacgg catcctcgcc gcgttgcgca ccgaaaggtc gatcagcaat
     6781 aaatcattga tgatgggaaa tgagagcacc gaagaatcca gaaatgcaat caggaaaagg
     6841 ccaaatcctc cagccgtagc gacaatcgcc tggagccagt gaggcaaatg aaatttcggt
     6901 tgtggcagtt ccataaacga attttgcaag cgttcgattg tagcagagac atcctgcaaa
     6961 cgatctgaaa gcggaactcg ttgttatata atcgcgccca atgaaatctc aaaagaaccg
     7021 cattgtcctt aacggaaaca gacgtaacgc tacaattgct ttatgttcga ctggcttcgt
     7081 tgcgatacag agttttcggg ctgcattctc taatgcaacg cggaatcccc attggctgat
     7141 ttccttggaa cctatcgcac ttcccacatg ggcattggct gtcgttaacg ccagtttcta
     7201 tttatgcata gtctggaaaa tggcgatttt cttccgaaac gcccaacgcg aggagcgcct
     7261 agttataggc ggttggttcg gagttttttt tcttattccc atgcaacact tggtttccat
     7321 tcggggcgct aacgccatcc agtggctcaa ggcaactggc ctggccacgg cgttcgttgc
     7381 cgccgcatat atattggtaa aaagtcctgc gaataccgaa ggcgtcacaa gggcgacgaa
     7441 gcagagattt cttatttttt tatttctagt attagtttcg tttgtaatcg gagcgctact
     7501 ttacttcgtt ccgcgttaac ttcaattcgt gacgatatga ttcatgcttg acacaattgt
     7561 aaggcaatta gactattggc tttcaaaggc a
//
LOCUS       DASZMB010000109        19019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_389069, whole genome shotgun sequence.
ACCESSION   DASZMB010000109 DASZMB010000000
VERSION     DASZMB010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 19019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19019
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389069"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1268)
                     /locus_tag="VG272_04965"
     CDS             complement(<1..1268)
                     /locus_tag="VG272_04965"
                     /inference="COORDINATES: protein motif:HMM:TIGR04519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HWF13065.1"
                     /translation="MDSDRKRAAQPDVDISVLRKAANAGNTPQFWRGLDELADTPEFQ
                     NHKENEFPHGATDTEAKLDRRELMKVMAASAAFAGLTGCTKLPTQKIVPYVKQPEQII
                     PGKPLFYATAVTLGGVATGVLVESHMARPTKIEGNPDHPGSLGATDAFSQASVLGLYD
                     PDRTQAEIHDGRIGSYAEFQEELSSALGQEKGSGGGGVRLLTPTITSPTLGDQISTFL
                     KAYPSAKWIQYESVTRDNIREGAKMAFGEYANPVYHVDQADVIVALDSDLLTSGPGSI
                     RYAREFARKRRVEGTESKMNRLYSVESTPTNTGGMADHRLRLKASEVEGFARLLAREI
                     GLSAVAAPAVTPSIPTGWIPALVRDLKQHPGATLIVAGDQQPAIVHALAHAMNQALGN
                     IGKTVHFTAPLEVVAENQWDAMGQLAADIRT"
     gene            complement(1316..1966)
                     /locus_tag="VG272_04970"
     CDS             complement(1316..1966)
                     /locus_tag="VG272_04970"
                     /inference="COORDINATES: protein motif:HMM:NF014176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HWF13066.1"
                     /translation="MAQIFQHSTNTISRVSIYGAVILIAILGYAANVVNQTSYITEVH
                     NALPQPVPFSHKHHVAELGLDCRYCHTSVEVSSSAGMPPTQTCMTCHSQIWTGAAILE
                     PVRVSYRDSTPISWTRVNALPDFVYFNHSIHINKGVGCTTCHGPVGEMNITWRENSLY
                     MRWCLECHNAPEKYLRPRSEVFNAFYKAPSDQEALGNRLMTDYKVQKLTNCTTCHR"
     gene            2570..5743
                     /locus_tag="VG272_04975"
     CDS             2570..5743
                     /locus_tag="VG272_04975"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWF13067.1"
                     /translation="MWIVRIALRRPYTFIVLALIIVLMTPIVLQRTPTDVFPDIDIPV
                     VAVAWNYSGLSPQLMEDRIVSPYERFLTTVVDNVEHTESQTVAGRSVIKVFFQPGADV
                     HVGVTQITALSQTIIRQLPPGISPPLIITYSASSVPVLQLGMRGDGLSEQELFDFGAN
                     LVRNQLATVQGAAIPWPYGGKQRQVSVNLDIPALQAKGLSPTDVITAISAQNLVLPSG
                     TVKMGSTEFNVEMNGSPDTLAALNNLPIKTANGATIYVKDVAYVSDGFSPQINIARMD
                     GQRGVLMAIYKTGATSTLKVVSEVYARLPIIKNLVPPQTVITPLFDQSIFVRAAIQGV
                     VREGVVAACLTALMILLFLGSWRSTLIIGISIPLSILISITILSMLHETINLMTLGGL
                     ALAVGILVDDATVEIENINRNLAQGKQIIQAILDGAQQIAIPAFVSTLSICIVFVPMF
                     FLTGVAKFLFVPLAEAVVFAMLASYLLSRTLVPTMAMYLLKEHHGEEYATGNDVFSRV
                     QRGFTHGFDRMRDGYRKSLTFCIERAWLFVILFLVFCVVSLAFIPVLGRDFFPTVDAG
                     LIRLHMRARAGQRVEETAREADGVDNLIRSVIPPEDLGNILDNIGLFNSTVNTVYSNS
                     GVIGESDAEILIGLKPERKESTKHYIDELRGRLAKEFPGTQFFFQPADMISQILNFGV
                     PAPVDVELIGPNEKANFLIAEQITNRIQHIPGAVDVHVQQLRSFPAIFLNLDRTRVQS
                     VGLSPTDVANSVLLTLSSSSTVSPSFWVNPMNGYEYNVAVQAPQYKIDNMQSLDNIPI
                     SSAANPTPQILGNMVGMSVNAEPALLSQYDAQSMMNVYASVEGRDLGGVDADIQKVLA
                     DFKGKLPRGTQLFRRGQVATMTSSFAGLTAGVAVAIVLIYFLIVINFQSWIDPFIIIT
                     ALPGALAGIIWILLLTHTTLNVPSLTGTIMCMGVATANSILMVSFARERMNEGLGAAQ
                     SAVQAGYVRIRPVIMTAVAMIIGMVPLSLGLGEGGEQNAPLGRAVIGGLIVATFATLY
                     FVPCVFSLIHKNKRLEESVSAAELGIN"
     gene            5775..7013
                     /locus_tag="VG272_04980"
     CDS             5775..7013
                     /locus_tag="VG272_04980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWF13068.1"
                     /translation="MSENHVELQDESKFAGEPGGSGRGPGALLRWFLIVFVIFAILGI
                     YAVSQRMSEHKALAEQTEQTAVPSVSVIHGTPIDTDSEMVLPGSLKPNVESPIYARTN
                     GYLKKWYKDIGSHVQKGEILAEIDTPEIDQQLAQSRAELVTTQANLNLSKLTATRYQD
                     LIKTDSVSRQDLDNANGDFAAKQAMVQSADANVKRLEELESFKRVYAPFTGIITQRNV
                     DPGTLINAGNGGSATKEMFDLAQIDPMRVFVAVPQSYSPSIHTGLKACLTLTELAQRN
                     FCGQVVRTANSIDPGTRTLLTEVDVPNPAGTLLPGAYAEVHFDVKVVAQRLSLPINAI
                     LFRPDGSMAAVVGTDNRINLKKITIGRDFGNALEILDGVSPTDRIVINPPDALEQGEE
                     VNIAAQNSAPSPASGASSKQ"
     gene            complement(7093..8337)
                     /locus_tag="VG272_04985"
     CDS             complement(7093..8337)
                     /locus_tag="VG272_04985"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HWF13069.1"
                     /translation="MNILVLNAGSSSLKCQIIATDLERIRQNKDARLMRGEVERIGGE
                     AVVTVQKGDSPKQLLTASLKDMSAALDFLIRWAASPESGVSEIQSIADIHAVGHRVVH
                     GGESFSDSVLINGDVLADIEKCIDLAPLHNPINLRGIRATTDLLGPKVPQVAVFDTAF
                     HHSLAERSYLYAIPYHLYLRYRIRRYGFHGISHRYMAYRYRAIRNLQPEQTNIISLHL
                     GNGCSAAAIRGGKSVDTSMGLTPLEGLVMGTRSGDLDPAVLGFIAAKEGISIHEVETM
                     LNKHSGLLGISGLTHDMRVLQQQADEHNDRRAKLAIEMFCYRARKYIGAFLAAMGGAD
                     SIVFTGGIGENSPAVRAGICEGLEWAGLALDNEKNQKTVGVEGPITKVDSRLAVYVIP
                     TDEELLIARDTARCILGEKNPQ"
     gene            complement(8534..10936)
                     /locus_tag="VG272_04990"
     CDS             complement(8534..10936)
                     /locus_tag="VG272_04990"
                     /inference="COORDINATES: protein motif:HMM:NF003621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HWF13070.1"
                     /translation="MAVTYTPGSIKVFSSSNPLSDEEKSRMNAYWRAANYLSVGQIYL
                     LANPLLREPLKLEHIKPRLLGHWGTTPGLNFIYVHMNRLIKKYDLDAIYIAGPGHGGP
                     GMVANTYLEGTYTELYPNIQESEEGMRRLFKQFSFPGGIPSHAAPETPGSIHEGGELG
                     YSIAHACGAAFDNPGIFVCCVVGDGEAETGALAASWHSNKFMNPARDGAVLPILHLNG
                     YKIANPTILARMSDGELAQLFAGYGYDPYFVEGHDPDAMHQLMSGTLDTVVAEIRSIQ
                     GKARHEGVKDRPLWPMIIMRTPKGWTGPRVVDGKPVEGTWRAHQVPLAELATKPEHLR
                     ALEEWMRSYRPEELFDHDGNLIRELAELAPVGNRRMGANPNANGGLLMKDLAMPDFRD
                     YSVPVVKPGTQNAEATKELGKFLRDVMRQNNETKNFRVFGPDETASNRLDAIYGATDK
                     IWMEPLLPTDENLSTDGRVMEVLSEHMCQGWLEGYLLTGRHGFFSCYEAFIHIVDSMF
                     NQHAKWLKVTNDIPWRRPIASLNYLLTSHVWRQDHNGFTHQDPGFIDLVVNKKADVVR
                     VYLPPDTNTLLSVADHCLRSRQYVNVIVAGKQPALQYLDMQSAIQHCTAGIGIWDWAS
                     NDQDGEPDVVVASAGDIPTLEALAAVDLLRQHFTDLRIRFINVVDLMTLQPESEHPHG
                     LPDKEFDSMFTVDKPIIFAFHGYPWLVHRLTYRRTNHDNLHVRGYKEEGTTTTPFDMT
                     VMNDMDRFHLASDVVDRVPRLQRIGAHFKQMLRDKLIDHKEYIMQVGDDMPEIKNWKW
                     PY"
     gene            complement(11481..12584)
                     /locus_tag="VG272_04995"
     CDS             complement(11481..12584)
                     /locus_tag="VG272_04995"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF13071.1"
                     /translation="MNRETLTVENEILDPRTDGHSHSRRKVLRMLSAAGMGLAISPRS
                     FAQKPGSGPGIIDTHHHIYPPKHTANNLKRIVDDAPAVPSSLYTNWTPHYSLDQMDQN
                     GVSTAIASISSPGVWFGNNEEGRRNARSVNEYGAQLGKDFPGRFGMWGAIPLPDIDGS
                     LREIEYIFDVLKLDGIGLLSSYENGKLLGNVKYAPVMDELNRRKAVVFVHPTVTCCGD
                     PIPRVNITSIEFPTDTTRTITDLVYSGTLMRCPDIRFIFSHGGGTILMLLARLSGGAL
                     TPEERNATIPNGFEHELKKLYYDIASVAVSPTAMTAVFSVIPKERLLFGSDIPFWTIE
                     KIATAMNKFDISAADLRMIQRENALKLVPRFKS"
     gene            complement(13059..13301)
                     /locus_tag="VG272_05000"
     CDS             complement(13059..13301)
                     /locus_tag="VG272_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13072.1"
                     /translation="MKSFEVQFSFRDRKEETVSSTVIVDASSLPGGVAKATREFVKGL
                     DRKQRFDMNKSGLEIMAKPSGSAAETPEKSAAEAAG"
     gene            complement(13393..13674)
                     /locus_tag="VG272_05005"
     CDS             complement(13393..13674)
                     /locus_tag="VG272_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13073.1"
                     /translation="MTRKSTLASLVVAIGLSLSLSACKDTKTLQENEQLKAQVADLQK
                     QNGELGNNLETVTAARDALIKENDSLKAKLRAPKAKTAKKKVASRKRRK"
     gene            complement(13866..15329)
                     /locus_tag="VG272_05010"
     CDS             complement(13866..15329)
                     /locus_tag="VG272_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWF13074.1"
                     /translation="MKTSHPIRYKQFATGKWEAILSMVGCMLFAAGIHGQTKQAAHPS
                     DPLHQLNDSVEALVQRVSPTIVQIQVSGYASSEDVGQGQASVIVGRQKTIGSGVIVDP
                     DGYIVTNAHVVKGAQKIEVIVPPATVSDKSSDNAQNGRERIFDARVVGIANQLDLAVI
                     KIEAHTLPALSIRGTVQARQGEMVFAFGSPEGLRNSVTMGVVSAVARQPDPDSPLVYV
                     QTDTPINPGNSGGPLVNADGDLVGINTFILSSSGGNQGLGFAIPAGVLAYAYPQLVKY
                     GHIHQPEIGAILQSITPELSAGLHLARDFGVIVSDVTPGGPAEQAGLKVQDIIVRVDD
                     MRTENLPLFGQSLYMHGSGDHVKLEVLRGSTRMDLDVSLGERPHKVDSLVDSVDPAKN
                     LVPRLGIIGVELNVNLAHSLPELRIPSGVIVAAKTLGAGSGEVPLQTGDVIHGLNGTT
                     VTGLSDLKDGLAKLAPGDSVVLWIERYGQLIYVSFVI"
     gene            15446..16282
                     /locus_tag="VG272_05015"
     CDS             15446..16282
                     /locus_tag="VG272_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13075.1"
                     /translation="MKTRKLFPIAFLALAYVGLAGNAASQSAQQSAPQSQKQKSGDNS
                     GQQDMPGMEDGMPGMDMSHDMPGMKMGQSGSDQQTEHGAMNAMSHMHHHQMGPHMYMT
                     KLRTATPQDWAKADEIVTELRESIEKYKDYHVALADGFRIFMPNLPQAEYHFTNYYNG
                     YLESFSFDPTRPTSLLYKKTKNGYDLIGAMYTMPKRVSEEQLNERVPLGVAQWHLHTN
                     LCMPPKEQRGHADLTKFGLQGSILTKDSCTEAGGIFYPVIFGWMVHVYPYEDSREKIW
                     GQ"
     gene            16424..17194
                     /gene="tmk"
                     /locus_tag="VG272_05020"
     CDS             16424..17194
                     /gene="tmk"
                     /locus_tag="VG272_05020"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HWF13076.1"
                     /translation="MTSDSLPAVDQLPSNHVEIAEESSEKLPPSKKYPGALIVVEGID
                     GSGKSTQLYLLKRWLEINGYRLHFTEWNSSPLVKSATRRGKQRRLLTPTTFSLLHAAD
                     FADRCERQILPLLHSGYLVLADRYIYTAFARDVARHCSPRWLRNLYRFAPVPDITFYF
                     RAPLDVAVKRIMSGRPKLKYYEAGMDLNLSLDRAESFRIFQGRILAQYDEMVESDKFV
                     VMDGTLPVNSLQKQMRQMVKARIDLSRFAPKKSARRPL"
     gene            17191..17988
                     /gene="tmk"
                     /locus_tag="VG272_05025"
     CDS             17191..17988
                     /gene="tmk"
                     /locus_tag="VG272_05025"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HWF13077.1"
                     /translation="MNTAKFSKKKERTKKGLIPTGAIPRNYGLPLPGISDEEFGGSLI
                     VLEGMDGSGRSTQIALLTEWLESEGFAVETMGLRRSGLVAKDIDALLAENTITRMTLT
                     LMYATDFYDQLEHRIIPALRSDFVVLADRYFYTLIARAGARGIQRDYLNGLYEMAVRP
                     DLTFWLSVRPEIAFEREFRKSHTISYWESGRDLSLSDDLFQSFILYQAKIKKEFESLS
                     QRHGFLSLDGEASVISVNNELRKQIAAHLGIQRTHYRPSSALASLWR"
     gene            17979..>19019
                     /locus_tag="VG272_05030"
     CDS             17979..>19019
                     /locus_tag="VG272_05030"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13078.1"
                     /translation="MALRLRKPIHLAAIDAGSNAFRLMVARIHSVDDWEIVESVRAPV
                     RLGHSVFTAGQFDRKTLREATSAFREFRKILDAHDVTAYRAVTTSAAREARNRHVLIE
                     RIRHQAKIELEVISGEEEARLVRVAVLRALRALDPPRYILDLGGGSLEINVLRRGVLQ
                     EIIGMPLGTVRLMEAFHAQGAIDDKTAEDIRRHVMSVLRKTLPNRPNLPGACTVACGG
                     NAETLATLTLGPRVGGFGTLNSRLLQDRMERILKSNVEERMKSFSVRRDRAEVMGVAA
                     IVLAAVASWLRVRQMITPRVGVREGLLYELASVAMAIPALTEEERTHSEAMISGAMSY
                     AHKVRCDLKHSEQ"
BASE COUNT         4306 a         5158 c         5058 g         4497 t
ORIGIN      
        1 gtgcgaatat cggcagccag ctggcccatg gcatcccatt gattttccgc gacaacttca
       61 agaggtgcgg tgaaatgaac cgtcttgccg atgttgccca gcgcctgatt catggcatga
      121 gcgagtgcat gcacgatggc cggctgctgg tctcccgcca caatcagggt cgctccggga
      181 tgctgcttca gatcgcgaac gagcgccgga atccagccgg tcggaatgct tggagtcacc
      241 gccggggctg caaccgcact caaaccaatt tcacgggcaa gcaggcgcgc gaagccttcc
      301 acttcagaag ctttcaagcg caagcggtgg tcggccatgc cgcctgtgtt cgtcggcgtc
      361 gattcaaccg agtacaggcg attcattttc gattcggtac cctcgacgcg gcgcttcctc
      421 gcaaactcac gggcgtaacg gatgcttcct ggcccactgg tcagtaaatc cgaatcgagc
      481 gcaacgatga cgtccgcctg atccacgtgg taaaccgggt tcgcatattc gccgaaggcc
      541 atcttggcgc cctcgcggat attgtcgcgt gtaaccgatt cgtattggat ccacttagcg
      601 gaaggatatg cctttaaaaa tgtgctgatc tggtcgccga gcgtagggga agtaatcgtc
      661 ggcgtcagga gccttacacc acctccgccg cttccctttt cctgtccaag cgctgatgac
      721 aactcttcct gaaactctgc gtagcttccg atgcgcccat cgtggatttc cgcctgcgtg
      781 cgatccgggt cgtacagacc caagacggaa gcctgcgaaa aagcgtcggt cgcgcccaag
      841 ctccccggat gatcgggatt gccttcaatc tttgtgggcc gggccatgtg gctttcaacc
      901 aggacacctg tggctacgcc accaagcgta acggctgttg catagaacag gggcttgccg
      961 ggaataatct gctcaggctg cttcacgtaa ggaacaattt tttgtgtggg aagtttggtg
     1021 cagcctgtga gcccggcaaa tgcagccgag gccgccatga ctttcattaa ttcgcgacgg
     1081 tcgagtttcg cctcggtatc cgttgctccg tgcggaaatt cgttttcctt gtgattctga
     1141 aattccggcg tgtcggccaa ttcatccagg ccgcgccaaa attgaggagt gttgcctgcg
     1201 ttcgcggcct tgcgtaaaac agaaatgtct acgtcgggct gagcggctct ttttcgatcg
     1261 ctgtccatga attccctgat ccctcatctg gcgggctact aagagcccgc acgatctagc
     1321 ggtggcatgt cgtacagttc gttagcttct gcactttgta atcggtcatc aggcggttgc
     1381 ccaaggcttc ctggtcagac ggagccttat agaacgcgtt aaacacctcg gaccgggggc
     1441 gcaaatactt ttccggcgcg ttatggcact cgaggcacca gcgcatatag agactgtttt
     1501 cgcgccaggt gatgttcatt tcacctacag ggccgtggca cgtggtgcag ccgactccct
     1561 tgttaatatg aatgctgtga ttgaaatata caaagtccgg gagcgcattg acccgcgtcc
     1621 aggaaatcgg cgtcgaatcg cgatagctca cacgcacagg ctcgaggatc gccgcgccgg
     1681 tccatatctg cgaatggcag gtcatgcagg tctgggtggg gggcataccc gccgaggaag
     1741 aaacttccac tgaggtatgg caataacggc aatccaagcc aagttcagcc acatgatgct
     1801 tgtggctaaa aggcacaggc tggggtagcg cgttatgcac ctctgtgatg taggaggtct
     1861 ggttcacgac gttagcggca tatcccaaaa tcgcgatcag gataaccgcg ccgtaaatgc
     1921 tgacgcgaga aatggtattt gtgctgtgtt gaaaaatttg cgccatacgt gtcgcccctc
     1981 ggaacacacc ccgaggtgca tgccaaaaaa gtccttagcg cctctctatc gaccgcattt
     2041 ccggccttga tttaacttcc gggacggcgc ttgctaactg catggaaggg ggatacaagc
     2101 cggcagtggt gcgtacatta tattccgccg atctttacta cacgggtaaa tctaaaattc
     2161 catttataat ttgtttttaa tatccccacc cgcgggaaat actataacag caacacaaaa
     2221 ataatttcaa tcgttgaatt aaattaatcg cgacaaaaca aacaacgggt tgtgatgcgg
     2281 atcactgatt tcgcagtcct gcttggggtt cagccgaatt gtccggcatt aaactttgtg
     2341 aacttatttt ccggacacga aatgcaagac ggcatttcat tcaagcgttt gcaaaaaaac
     2401 tcaaccgaag tgagtcgaga gagcgctatc atccaagaag atatgtaacg gaaagtttga
     2461 ccggggcatc taattaggaa cagtttcata acgataaact ccggtgccgg agttcacctt
     2521 gtcggttcat tcgcggtaag attctatgca ctctcgaggt cattaaagaa tgtggattgt
     2581 ccgaatagcg ctgcggcggc cgtacacgtt tatcgtgctg gcgctaatca ttgtgctgat
     2641 gacgccaatc gtgttgcaac gcacgccgac cgatgtgttc cccgatatcg atattcccgt
     2701 ggtggcagtc gcatggaatt attcgggttt gtcgccccaa ttgatggaag atcgaatcgt
     2761 ttccccgtat gaacggtttc taactacggt agtagataac gtcgagcata cggaatcgca
     2821 aaccgtggcc ggacgctccg tcatcaaagt attttttcaa ccaggcgcgg atgttcacgt
     2881 aggggttacg caaatcaccg cgctctccca aaccattatt cggcaattgc caccgggcat
     2941 ttccccgccg ctgattatca cgtattcggc ttccagcgtg cccgtgctgc aactgggcat
     3001 gaggggggat ggattatccg agcaggaatt atttgatttc ggagccaacc ttgtacgcaa
     3061 tcaattggcg accgtccagg gcgccgcaat cccctggcct tatggaggaa agcagcggca
     3121 agtctccgtg aacctggata ttccggcctt gcaggcaaaa ggtttatcgc cgacggatgt
     3181 gatcaccgcg atctccgcgc aaaacctggt actgccttca ggcacggtca agatggggtc
     3241 gactgaattc aacgtggaaa tgaatggatc gcccgatacg cttgccgcgc tgaacaattt
     3301 gccgataaag accgctaatg gcgcaaccat ttatgtaaaa gatgtcgcct atgtgagcga
     3361 cggcttttcg ccacagatca atatcgcacg catggatgga cagcgtggag tattgatggc
     3421 tatctacaaa accggcgcaa cctcgacact taaagtcgtg tcggaagtgt acgcgagact
     3481 gcccatcatc aagaatctcg ttccgccaca aacagtgatt actccactgt tcgaccaatc
     3541 tattttcgta cgggccgcta ttcagggagt cgttcgagag ggtgtggttg ccgcctgcct
     3601 gaccgcgctg atgattctgc tgtttttggg cagctggcgc agcacactaa tcatcgggat
     3661 ttcaattcca ctttcgatcc tgatctccat cacgattttg agcatgcttc atgaaaccat
     3721 caacctcatg acgctgggcg gattggctct ggctgtcggc attctggtgg atgacgccac
     3781 ggttgaaatc gaaaacatca atcgaaatct tgcgcaagga aaacagatta ttcaagccat
     3841 cctcgatggc gcgcagcaaa ttgcaatccc tgcgttcgtg tccacgctgt cgatctgcat
     3901 tgtgtttgtg ccgatgtttt ttcttacggg agtcgcgaaa ttcctgttcg tgccgctggc
     3961 cgaagccgtg gtgttcgcga tgctcgcctc ttatctccta tcgcgaacgc ttgtgccaac
     4021 catggcgatg tatctgctaa aggaacatca tggcgaagag tacgccacgg gaaacgatgt
     4081 tttctcgcga gtgcagcggg gattcacgca tggattcgat cgcatgcgtg atggctatcg
     4141 caaatcgctt actttttgca tcgaacgcgc gtggctgttt gtcatcttgt ttttggtctt
     4201 ttgcgtggtt tcgctggctt tcattcctgt gctgggccgt gacttcttcc ccacggtgga
     4261 tgcggggctg attcgattgc acatgcgcgc ccgcgccggg cagcgcgtgg aagaaaccgc
     4321 tcgagaagcg gatggcgtgg acaatttaat ccgcagtgta attcctccgg aggacctcgg
     4381 aaatattctc gacaacatcg gcttgttcaa cagcaccgtg aatacggtct acagcaattc
     4441 aggcgtcatt ggcgaatccg atgcggaaat tctaattggt ctgaaacctg aacgcaaaga
     4501 gtcaacaaaa cattacatcg atgagttgcg tgggcgtctg gccaaggaat ttcccggcac
     4561 gcaattcttt tttcagcccg cggatatgat cagccagatt ttgaatttcg gagtgcctgc
     4621 cccagtcgac gtcgaactga tcgggccgaa tgaaaaggca aattttctga ttgcggagca
     4681 aattacaaat cgcattcagc acattccggg cgcggtagac gtgcacgtac aacagctgcg
     4741 atcttttccc gccattttcc tgaatctgga ccgtacgcgc gtgcaatccg tcggcctaag
     4801 cccgacagat gtggccaaca gcgtcctgct tacgctgagc tccagctcaa ctgtttcgcc
     4861 gtcgttctgg gtcaatccta tgaacggtta tgaatacaac gtggccgtgc aggcgccgca
     4921 atacaaaatc gataatatgc aatcgctcga taatattccc atctcttcag ctgcaaatcc
     4981 gacgccgcaa atattgggaa acatggtggg gatgagcgta aacgccgagc cggcgctgct
     5041 cagccaatac gacgcgcaat ccatgatgaa tgtttacgca tcggtggaag ggcgcgacct
     5101 gggtggcgtg gatgcggata tccagaaagt attggcggat tttaagggca agcttccgcg
     5161 cgggacgcaa ctattcaggc gcggtcaagt cgcgacgatg acgtcttcgt ttgccggact
     5221 tacggcaggc gtggccgtcg ccatcgtttt aatttatttt ttgatcgtga tcaattttca
     5281 atcgtggatc gatcctttta ttattattac cgcgctgcct ggggcgctcg ccggcatcat
     5341 atggattttg ctgctcacgc ataccacgct gaacgtgcca tccttgactg gcacgatcat
     5401 gtgcatgggc gtggccacgg cgaatagcat cctcatggtt tcctttgcgc gcgagcgaat
     5461 gaatgaggga ttgggcgccg cgcaatcagc cgtgcaggcc ggatatgtgc gtatccggcc
     5521 cgtgatcatg accgcggttg ccatgatcat cggcatggtt cccctttcgc tcggactggg
     5581 agaaggtgga gagcaaaacg ccccgctagg ccgggccgtc atcggtggat tgattgtggc
     5641 cacgttcgca actttatatt ttgtgccttg cgtattttct ttgattcaca aaaacaagcg
     5701 tctggaagaa agcgtcagtg ctgcggagct tggcattaat tagcgggctt atgagttcag
     5761 ggagcaaact gggaatgtct gaaaatcacg tcgagttgca ggacgaatca aaatttgccg
     5821 gcgagccggg cggatcaggc agaggtcccg gagcgctgct tcgctggttt ttgattgtat
     5881 ttgttatttt cgcgattctt gggatatatg ccgtctcgca gcgcatgagc gaacataagg
     5941 ctctcgcgga gcaaacggaa caaacggccg ttccatctgt ttcggtgatt cacggaactc
     6001 cgattgatac cgattctgaa atggtgctcc ccggctcgct caagcctaat gtcgagtctc
     6061 cgatttatgc caggacgaat ggctacctga aaaaatggta caaggatatt ggcagtcacg
     6121 tgcagaaggg cgaaattctt gccgaaatcg atacgccgga aattgaccag caactggccc
     6181 agtctcgcgc cgaactggta accacccagg cgaatctcaa cctttctaaa ttaacggcca
     6241 cacgctatca ggatttgatc aagacagatt cagtttcgcg ccaggatctc gacaacgcga
     6301 atggcgattt cgccgcaaag caggccatgg tgcagtcggc agacgccaat gtaaaacggc
     6361 tggaagaatt ggaatcgttc aagcgcgtgt atgctccgtt cacgggaatc atcacgcaga
     6421 gaaatgtcga tcccggaacg ctgatcaacg cgggaaatgg cgggtcggcc acgaaagaaa
     6481 tgttcgatct ggcgcagatt gatccgatgc gcgtgtttgt cgctgtgccg cagtcttact
     6541 cgccttccat tcacacgggg ctgaaggcct gcctcacgct gacggaactt gcgcagagga
     6601 atttctgcgg acaagtcgtg cgcaccgcta attccatcga tcctgggacg cgaactttgc
     6661 tcaccgaagt ggatgtcccg aatcctgctg gaacactttt gcccggggca tacgcggaag
     6721 tccatttcga tgtgaaggtc gttgcgcagc ggctttccct gccgattaat gcgattttgt
     6781 ttcgcccgga cggatcgatg gcagcggtcg tgggcacgga caaccgcatc aaccttaaaa
     6841 aaattacgat tggacgggat ttcggaaacg ctttggaaat cctcgatgga gtttctccga
     6901 cggaccgcat cgtgattaat cctccggacg cgttggagca gggcgaagag gtcaatattg
     6961 ccgcgcaaaa tagcgcgccc tcgcctgcat ccggcgcttc ttccaagcag tagcgatccc
     7021 actttttttg agggaactta aattttgcat ttgcgccggt tttactttcg caaattgtcc
     7081 tggggggaat atttactgcg gatttttttc gcccaggatg cagcgcgctg tatctctggc
     7141 gatcaatagt tcttcgtccg tagggattac gtaaacggcc aggcgcgaat ccactttcgt
     7201 gatgggcccc tcaacaccaa ccgttttttg attcttttca ttgtccagcg ctaatccggc
     7261 ccactcgagg ccttcgcaaa ttcctgcgcg taccgccggc gaattttcac cgatgcctcc
     7321 ggtgaacacg atggaatccg ctccgcccat tgcggcaaga aatgcgccga tatattttcg
     7381 cgcccggtag cagaacattt cgatggccag ctttgcacgg cggtcattgt gctcatccgc
     7441 ttgctgctgc aaaacgcgca tgtcgtgcgt gagtccggaa attcccagca agccggaatg
     7501 tttgttcagc atggtctcta cttcatgaat ggaaatccct tccttcgcgg caataaatcc
     7561 caatacagcc ggatcgagat cgcccgagcg cgtgcccatc accaggcctt cgagcggcgt
     7621 caagcccatc gaggtatcca cagatttacc gccgcgaatg gccgccgccg agcagccgtt
     7681 tccgagatgc aggctgatga tgttggtctg ctcgggctgc aaattgcgga tggcgcggta
     7741 acgatacgcc atgtaacgat gtgaaatgcc gtggaatccg taacgccgga ttcgatagcg
     7801 aagatatagg tgatacggaa ttgcgtagag ataagagcgc tcggcgagcg aatggtgaaa
     7861 tgccgtatcg aacaccgcga cttgcggaac tttaggaccg agcaaatcag ttgtcgcgcg
     7921 gatgccgcgc aggttaatgg gattgtgcag tggagcgagg tcgatgcact tttcgatgtc
     7981 cgccagaaca tccccgttaa tcaaaacgga gtcggaaaac gattcgcctc catgcacaac
     8041 gcgatgcccc accgcatgaa tgtcagcgat gctctgaatt tccgaaacgc cggattccgg
     8101 cgaggcggcc cagcgaatca ggaagtcgag cgccgcggac atatccttca gcgatgcggt
     8161 gagtaattgc ttggggctgt cgcccttttg cacggtgacg accgcttccc cgccaatgcg
     8221 ctcgacctcg ccgcgcatga gacgagcatc tttattctga cggatgcgtt caagatccgt
     8281 tgcgatgatt tggcacttga ggctcgaact gccggcattc agaacgagaa tgttcattcc
     8341 gattttttcc acacgctttt tttcctcgtg ctcagataaa taaggatcac aaggtatttc
     8401 acaaatgact cgccgcaggt actgggtagt gtttcagggg ctaaagcccg tgtttttcaa
     8461 ctctctgaac gtcgcggctg aagccgcaac ccacaaagac gctctatgag gccgcctcca
     8521 attgaaaccc agatcagtag ggccatttcc agttcttaat ttccggcatg tcatcaccaa
     8581 cttgcatgat gtattccttg tggtcaatca atttgtcgcg cagcatttgt ttgaagtgcg
     8641 ctccgatccg ctgcagtcgc ggcacgcggt caaccacatc cgaagcgaga tgaaatcggt
     8701 ccatgtcgtt catcaccgtc atgtcaaacg gcgttgttgt tgttccctct tccttgtagc
     8761 cacgcacgtg gaggttatcg tggttggtcc ggcgatacgt cagccggtga acgagccatg
     8821 ggtagccatg aaacgcaaaa ataattggct tgtccaccgt gaacatggaa tcaaactcct
     8881 tgtccggcaa tccgtgcgga tgttcactct ccggttgcag cgtcatcaaa tccaccacgt
     8941 tgatgaagcg aatgcgcagg tccgtgaaat gttgacgcag cagatccacc gcggcgagcg
     9001 cctcgagcgt aggaatatct cccgcactgg cgacgacgac gtccggctcg ccatcctgat
     9061 cgttgctcgc ccaatcccaa atgccaattc cggcggtgca gtgttgaatg gcggattgca
     9121 tgtcgagata ctgcaaagca ggctgcttcc ccgcgacgat cacgttcacg tactgacggc
     9181 tgcgcaaaca atgatcggcg acggagagca gtgtattcgt atcgggaggt aaatagacac
     9241 gcaccacatc cgctttttta ttgaccacaa gatctatgaa tcccggatcc tgatgcgtaa
     9301 aaccattgtg atcctgtctc caaacgtgcg aggtcagcag atagttgaga gatgcgatgg
     9361 gccgccgcca cggaatatcg ttggttactt tgagccattt ggcgtgctga ttgaacatgg
     9421 aatcgacaat gtggatgaat gcttcgtaac aggaaaaaaa tccgtggcgt ccggtcagca
     9481 aatatccttc cagccagccc tggcacatat gctcgctcaa aacttccatc acgcggccgt
     9541 ccgtagaaag attctcatcc gtgggaagca gcggctccat ccaaatttta tcagtggctc
     9601 cgtagatggc atcgagccgg ttggaggcag tttcatccgg accgaatacc cgaaagtttt
     9661 ttgtctcgtt gttctgtcgc atgacatcac gcaagaattt gccaagttct ttagtcgcct
     9721 ccgcattttg cgttcccggt ttcacgacgg gcacggaata atcgcggaag tccggcatgg
     9781 ccaggtcttt catcagcagg ccgccgttgg cgttgggatt tgctcccatc ctcctgttgc
     9841 ctacaggcgc cagctcggcc aattcgcgga tcaaatttcc atcgtgatcg aagagctcct
     9901 cgggacggta gcttctcatc cactcttcca atgcgcgcaa atgttccggt ttcgttgcga
     9961 gttcagcaag cggcacttgg tgcgcgcgcc aggttccttc cacgggtttc ccatccacca
    10021 cgcgaggccc cgtccagcct ttcggcgtgc gcatgataat catcggccat aacggacggt
    10081 ctttgactcc ttcgtgcctt gccttgccct ggatggaccg aatctccgca accacggtgt
    10141 cgagggttcc ggacatgagt tggtgcatcg cgtcaggatc gtggccctct acaaaatagg
    10201 gatcgtatcc atagcccgca aaaagctgcg ccagttcgcc gtctgacatt cgcgcgagaa
    10261 tcgttggatt ggcaatcttg taaccgttca aatggaggat gggcagcaca gcgccgtcac
    10321 gcgcaggatt catgaatttg tttgaatgcc agctggcggc gagagccccg gtttccgctt
    10381 ccccatcgcc aacgacgcaa caaacaaata tgccgggatt atcgaacgca gcgccgcacg
    10441 cgtgggcaat cgagtagccg agttctccac cctcgtgaat ggaacccggt gtttcgggag
    10501 ccgcatggct tggaatgccg cctggaaacg aaaactgctt gaagaggcgc cgcatgcctt
    10561 cttcggactc ctgaatattc ggataaagtt cggtatacgt cccttcgagg tatgtgtttg
    10621 ctaccatgcc cggaccacca tggcccggcc ctgcaatata gattgcatcc agatcatatt
    10681 ttttaatcag gcggttcatg tgcacataaa tgaagttcag tcctggcgtc gttccccagt
    10741 ggccgagcaa tcgcggtttg atatgttcga gctttaaggg ttcgcgcagc agcggatttg
    10801 cgagcaaata aatttgcccc acggagaggt agtttgcggc gcgccagtag gcgttcatcc
    10861 ttgacttctc ttcgtctgaa agtggattgc tcgatgaaaa aactttgatg gaaccgggcg
    10921 tgtaggtaac agccatttcg atctccttta taggccccgg ggcggcctcc tgagttccgg
    10981 actcgttgaa gtttatgcct cgacaaggag ggcggtttga ataacggatg aatatttagc
    11041 gggggtcgca atgcagtcag cggggactgc gtccaaataa ggagctggaa aagacgtcct
    11101 acgaaatctg gacggtttta tgattcgatg attgcctgcc ttcgcaatct gtttctcgtg
    11161 taagtcggtt acgtttttgg cgacttgtta ccgcggcatc tccttccgaa gccgcaaaac
    11221 aatctctcaa ataatctcct cgcatttatt tctgcgccac tgcggccagg ctaataaatt
    11281 ctacacttaa tttccgggaa tggcttttaa gtttcttggt gcgggctggg tgatctattt
    11341 ggagacgcct gaaataatat ggaaagaagg ctgcccgtgc aaaaagaatg gcggacgcta
    11401 caaaaacgaa tgcaacgcgt ttatagaggc ttcgctgtgc cgcgattttc gggacagaga
    11461 gcgcagtccg gctcggaggc tcaggacttg aaacgcggga ccagcttcag cgcgttctcg
    11521 cgctggatca tgcgtaagtc ggccgcggaa atatcgaatt tgttcatggc tgtggcgatt
    11581 ttttcgattg tccagaacgg gatgtccgaa ccaaacagaa gacgttcctt gggaatcacg
    11641 ctgaacacgg cggtcatggc cgtggggcta accgcgacac tggcaatatc gtaataaagt
    11701 ttttttaatt catgctcaaa tccattcgga attgtggcat tgcgttcttc cggcgtaagg
    11761 gctccacccg aaagccgtgc cagcagcatc aggatcgtcc cgccgccgtg cgaaaaaata
    11821 aatcgtatgt cgggacagcg catcagcgtt ccgctgtaga cgagatctgt aatcgtgcgc
    11881 gttgtgtccg tgggaaactc gatggacgtg atattcacgc gcggaatcgg atcgccgcag
    11941 caggtcaccg ttggatggac aaaaacaacg gccttccggc gattgagttc gtccatcacc
    12001 ggagcatact taacatttcc cagcaatttt ccgttttcat aactggaaag caagccgatc
    12061 ccatccagct tcaggacatc gaatatgtat tcgatttcgc gcaagcttcc gtcaatgtcc
    12121 ggcagcggaa ttgcgcccca cattccgaag cgcccgggaa aatccttccc tagctgcgct
    12181 ccatattcat tcacgctgcg cgcattcctg cgcccctctt cattatttcc gaaccaaacg
    12241 cccggactgg aaatggaagc gatcgccgtg gacacgccat tctgatccat ttgatcgagc
    12301 gaatagtgcg gcgtccaatt cgtatacaga gaacttggca cggccggagc gtcatctaca
    12361 attcgcttca ggttattcgc tgtgtgtttc ggcggataaa tgtggtggtg cgtatcgatg
    12421 atgccgggcc cgctacccgg cttttgcgcg aaggaacgcg gcgagattgc cagacccatg
    12481 cctgctgcgg acaacatgcg cagaacttta cggcgtgagt gcgaatgccc gtctgtgcga
    12541 gggtcgagaa tctcattttc caccgtcaat gtttctcgat tcaaaggatg ttcctttcca
    12601 cctcgaagaa atttcgtctg accgaagccg cacagattcg ggacattaac gcgaagcttc
    12661 tgatggaccg tacttccact gtgttctgaa gtcaagaata ttttggcggt gtttaagcat
    12721 ttgcttaaat tgtttattgc gtggcgggac cacggggttg cgtggcatag gcttgagagt
    12781 ctgggtgaga actgcggaat cgttgtgggc cgtagcttca gccacgacat aagtcctgca
    12841 aaatcagttc ggctttcgcc gctgaagttt caatattgga gttgttcact cagaccgttc
    12901 agcctgtggg gattagtgct agcgagatca aaacccgaga gggtgactgg tagaggacaa
    12961 aattccacag gctgaagcct acgccacgta attccaaatc tcagcaagcg cccactgagc
    13021 acatgacaca gctcaatggg cgtttgcaag gagatgattc agcctgcggc ttccgcggct
    13081 gacttctccg gcgtttccgc cgccgaaccc gacggcttcg ccataatttc cagaccgctc
    13141 ttgttcatgt caaagcgctg cttacggtcc aggcccttca caaactcgcg cgttgccttg
    13201 gcaacgccgc ccggcaggct ggacgcatcc acaattacgg tcgatgacac cgtttcttcc
    13261 ttacgatcgc gaaagctaaa ttgaacctcg aatgatttca tgatccactt cccccttttt
    13321 caaattttaa atttgtttga tccccgctct gcgaatcccg tgcagcagca aaaactagag
    13381 acatcttcca atctatttac gacgcttgcg gctggcaact ttctttttgg cggtttttgc
    13441 tttcggcgct ctcaacttcg ccttcagcga atcattttct ttgatcagcg catcgcgagc
    13501 cgcagtgact gtttcaaggt tatttcccag ttcgccattc tgtttttgca ggtccgcaac
    13561 ttgagccttt aattgttcat tttcttgcag ggttttcgta tccttgcacg cggacaggga
    13621 caaacttagc cctatcgcca caacaaggct cgccaaagta cttttgcgcg tcatgcgcca
    13681 tccttccatc gctagaattt gtactgcaac tggagctacg gtagcgggtg aggtgggaag
    13741 actccgccga caacccaaat agcgtactgg agatttccag cgggcgcaag ccggatcgct
    13801 atttattaca tttgtaatcg cggttgaggc ggaaaggaaa ttcgtggcaa taccttgaaa
    13861 ataaattaaa tcacaaagga cacatagatt aattgtccgt atcgctcgat ccataaaacc
    13921 acggaatcac cgggagcaag tttcgcgagg ccatctttca aatcgcttaa acccgtgacg
    13981 gtagtgccgt tcaaaccgtg aatcacgtct cctgtttgca atgggacttc tcccgatccc
    14041 gctcccaaag ttttggccgc aacaatcacg ccgctgggaa ttctcaattc aggtaacgag
    14101 tgggccagat taacattcaa ttccacgccg atgatgccaa gacgcggtac cagatttttt
    14161 gccggatcga ctgaatccac caaactatca actttgtgcg gccgctctcc caacgagaca
    14221 tcgagatcca tgcgagtcga accgcgaagc acttccaact tcacatgatc ccccgagccg
    14281 tgcatataaa ggctctggcc aaacaagggc aggttctctg tgcgcatatc gtccacgcgc
    14341 acgatgatgt cctgcacttt taatcctgcc tgttccgccg gcccaccagg ggttacatcc
    14401 gaaactatca cgccgaaatc gcgcgcgagg tgcagtccag cggaaagttc cggtgtaatg
    14461 gactgaagaa tggcgccgat ctccggctgg tgaatatgcc cgtattttac aagttgcgga
    14521 tacgcatagg cgagcacgcc ggcgggaatc gcaaatccca acccttgatt gccgcccgaa
    14581 ctcgacaaaa tgaaagtatt aattcccaca aggtcgccgt ccgcatttac gagtgggccg
    14641 ccgctattgc ccggattaat aggcgtatcc gtttgcacat aaacgagcgg gctgtccgga
    14701 tcgggctgcc gagcgacggc gcttacaact cccatcgtca cgctattgcg taaaccttcg
    14761 ggacttccaa atgcaaaaac catttcgcct tgtctggcct gcacggtgcc gcggatggat
    14821 aatgccggca atgtatgcgc ctcgatcttg atcacagcca gatcgagctg attggcgatg
    14881 cccacgacac gcgcatcaaa aatgcgctca cgaccatttt gcgcgttgtc agaagattta
    14941 tcggaaaccg tggcgggcgg cacaatgact tcaatcttct gcgcgccttt tacaacgtgg
    15001 gcattggtca caatgtagcc gtccgggtcg acaattactc ctgagcctat cgtcttctgc
    15061 ctccccacaa taacgcttgc ctgtccctga cctacgtctt cgctggacgc atatcccgaa
    15121 acttgaatct gaacgatggt gggcgaaaca cgctgcacca gcgcttccac ggaatcattc
    15181 agttggtgta aggggtcgga aggatgtgct gcttgcttgg tttgcccgtg aatgccagcg
    15241 gcgaaaagca tgcatccaac catggagaga atggcttccc attttcccgt ggcgaactgt
    15301 ttgtatcgaa taggatgtga ggtcttcatg gctcttctga ttagacgctt tttcctgggt
    15361 ccgggatacg ttcattttaa ggcaccccgt cgattgaaac ttttatgccg cgggcgcgta
    15421 gaataaggag tagaaggtac cagacatgaa gacacgaaaa ctgttcccca tcgcattcct
    15481 tgcgcttgct tatgttgggc tcgcggggaa cgccgccagc cagtctgcgc agcaatccgc
    15541 cccacaaagc cagaagcaaa aatccggcga caattccggc cagcaagata tgcccggcat
    15601 ggaagatgga atgcctggaa tggacatgag tcatgacatg cccggaatga aaatgggcca
    15661 gagcggcagc gaccagcaaa ccgagcacgg ggccatgaac gcgatgagcc acatgcacca
    15721 tcaccagatg ggcccgcaca tgtacatgac caaactgcgg actgcgacgc cgcaggattg
    15781 ggccaaggcc gacgaaattg tgacggagct gcgcgagtcc atcgaaaagt acaaggacta
    15841 ccacgtggcc ctcgctgacg ggttccgaat cttcatgcca aatcttccgc aggccgaata
    15901 tcactttacg aattactaca atggatacct cgaatctttc agcttcgatc ccacgcgccc
    15961 cacttccctg ctttataaga aaacgaaaaa cggctatgac ctgatcggcg cgatgtacac
    16021 catgcccaaa cgcgtttcgg aagagcaact gaacgaacgc gtgccactcg gcgtggcgca
    16081 gtggcacctg cacacgaacc tctgcatgcc tcccaaagag cagcgcggcc atgccgacct
    16141 cactaaattc ggcttgcagg gttctattct tacgaaagat tcctgcacgg aggcaggagg
    16201 aattttctat cccgtaattt ttggatggat ggtgcatgtc tatccttacg aggattcgcg
    16261 cgaaaaaata tggggccagt agcctctccc ttaaaacttg agttgcaagt ctcggaaggc
    16321 ccggcgcaac tctctcaaaa cggcccccca ttcatcttcc tctcatgttg ttgtcaccaa
    16381 atttttacat tgtgtgcgta taatggggcg acgggagacc tacgtgactt cggactcatt
    16441 gccagcggtc gatcaattgc cctctaatca tgtggaaatc gctgaagaat cgtccgaaaa
    16501 gcttccgcca tccaaaaaat atcccggtgc gctgattgtc gtggaaggca tcgacggctc
    16561 ggggaaaagc acgcaactat atttgttaaa gcggtggctg gagatcaatg gctatcgcct
    16621 ccatttcacg gaatggaatt catctcccct ggtgaagtcc gccacgcgcc gcggaaaaca
    16681 gcgcaggctc ctgactccca caacgttttc gctgctgcat gcagcggatt tcgccgaccg
    16741 ctgcgagcgc cagatcctgc cgctgcttca tagcggatac cttgtgcttg cggaccgcta
    16801 catctatacg gcatttgcgc gtgacgtcgc gcggcattgc tcccctcgct ggctgcgtaa
    16861 tctgtaccgg ttcgcgccgg ttccggacat cacattttat ttccgcgcgc cgctcgacgt
    16921 agccgtgaag cgaattatga gcggcaggcc gaaactgaaa tattatgaag cgggcatgga
    16981 cttgaatctt tcgctggacc gcgcggaaag tttccgaatt tttcagggcc gtatcctggc
    17041 gcagtatgat gaaatggtgg aatccgacaa gttcgtggta atggacggaa cgctgccagt
    17101 caattcgctg caaaagcaga tgcgccagat ggtcaaagcg cgtatcgacc tctcccgatt
    17161 cgcacccaag aaatccgcgc ggaggccatt gtgaataccg cgaaattcag caagaaaaaa
    17221 gaacgcacaa aaaaaggctt aattcccact ggcgctatac cgcgaaatta cggccttccg
    17281 ctgccgggta tcagcgatga ggagtttggc ggctcgctga tcgttctgga aggcatggac
    17341 ggatccggcc gctcgacaca aatagcgctg ctgacggaat ggctcgaatc ggaaggcttt
    17401 gctgtcgaaa cgatgggact gcgtcggtca ggccttgtgg cgaaagacat cgacgccttg
    17461 ctggccgaga acacgatcac gcgcatgacg ctcacgctca tgtatgccac tgatttctac
    17521 gatcagcttg agcaccgcat tattcccgcc ttgcgttccg attttgtggt tctggcggac
    17581 cgctactttt acaccttgat tgcacgcgcg ggcgcccgtg gtattcaacg cgactacctc
    17641 aatggccttt acgaaatggc ggttcgtccc gatctcacat tttggttgag cgtgcgcccg
    17701 gaaattgcgt tcgagcgcga atttcgcaaa tcgcacacca tcagttactg ggaatccggg
    17761 cgcgaccttt ccctatccga tgatttattt cagtccttca ttctctatca ggcaaaaatt
    17821 aagaaagaat tcgaatccct ctcgcagcgc cacggtttcc tttcgctgga cggcgaagcg
    17881 agcgtcattt ccgtcaacaa cgaactgcgc aaacaaatcg ccgcacacct cggcattcag
    17941 cgcacgcatt accggccatc ctccgcactg gcctccctgt ggcgctgaga cttcgcaaac
    18001 ccatacacct cgcggcgatt gacgccggct ccaacgcgtt tcgcctgatg gtcgcgcgta
    18061 ttcactccgt tgacgattgg gaaattgtgg agtcggttcg cgcgcccgtg cgtctcggcc
    18121 attcggtatt taccgccggc caattcgatc gcaagacgct gcgcgaagcc acttccgcct
    18181 tccgcgaatt ccgaaaaatc ctggacgcgc acgatgtcac agcctaccgc gccgtgacga
    18241 cgagcgccgc gcgcgaggcg cgaaatcgcc acgtattgat tgaacgtatt cgccaccaag
    18301 caaaaattga actggaagtg atttccggtg aagaagaggc gcgactggta agggttgcag
    18361 tgctccgcgc gctgcgcgcc ctggatccgc cgcgctatat cctcgacctc ggaggcggaa
    18421 gcctggagat aaatgttctg cgccgcggcg tcctgcagga aattatcgga atgccgctgg
    18481 gaactgttcg cctgatggaa gcatttcacg cgcaaggagc gattgacgac aagaccgccg
    18541 aagatattcg ccgccacgtt atgtccgttc tgcgaaagac gttgcccaac cgtcccaatc
    18601 ttccgggagc gtgcacagtt gcatgcggag gaaatgcgga aactcttgcg acgctcacct
    18661 taggccccag agtaggcggc ttcggaaccc tgaattctcg attgctgcaa gaccggatgg
    18721 agcggattct gaaatccaac gtggaagaaa gaatgaaatc gttttccgtc cggcgcgatc
    18781 gcgcggaggt aatgggggtt gccgccattg ttttggcggc cgtcgcttct tggttgcgcg
    18841 ttcgtcaaat gatcaccccg cgcgtgggag tgcgcgaagg acttctttac gaacttgctt
    18901 ccgtcgcaat ggcgattcct gcgcttacgg aagaggaacg aacacattcc gaagccatga
    18961 tctccggcgc catgtcctat gcgcacaaag ttcgttgcga cctgaagcat tcggagcag
//
LOCUS       DASZMB010000110         3931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1223367, whole genome shotgun sequence.
ACCESSION   DASZMB010000110 DASZMB010000000
VERSION     DASZMB010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223367"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..902)
                     /locus_tag="VG272_05035"
     CDS             complement(120..902)
                     /locus_tag="VG272_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13079.1"
                     /translation="MWNRIGTIALVGLATVSLSCVLHAQAAPQSGAAKSAGASATASA
                     PTSPRDFSGVWNMHVLPAQRKFLGSTYTAEPPDMTPWGNEKYMSYKPSNGPRTHSLTE
                     TDDPVLKLCLPPGTPRIYLQPFPFQIVMTPKEMIMIFEYDHTIRQIFLDGRPHPEDIT
                     PTYMGHSIGKWDGDTLVVDTVGFNERTWLDRDGHPHSDQLHIVERFHKTDGDNMVDDI
                     TIEDPKALVKPWKTQLNFQLKPGWDIMELACTDNVDFVNFEK"
     gene            complement(970..1356)
                     /locus_tag="VG272_05040"
     CDS             complement(970..1356)
                     /locus_tag="VG272_05040"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HWF13080.1"
                     /translation="MRNCRVSILALCIGLLALAPMAFAHHGTANYDTGRQITVKGTVT
                     GFEFVNPHVQIFWDAKDDAGAAQKWQGELTSPNRLSRVGWSKSTLKAGDAVTISGFPT
                     KSGSHEIWIQKVVTADGTELQTGGGN"
     gene            1633..2925
                     /locus_tag="VG272_05045"
     CDS             1633..2925
                     /locus_tag="VG272_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13081.1"
                     /translation="MRKVLLTVLTIGVGCVIGFSFGSNPWTVQGQGSGSIAAVAGQVG
                     GQDTFGAYDIDKNWPANISTVPGNDAWTWGAGQGIYAENPDRVFLLFRGELPNIKRPA
                     AKLIPEFGPSLIFPIGRLPWRDATISALPGAGGTGQDPDDGPKLWMEGKGVVGVDAKW
                     EHCLTIVDSKGNIVETFPQYDHLFKRPHYVTINPYDPEKHIWIVDDHMHAIYIFTHDM
                     KTLVKTMGTPTVKGADGTHFNRPTFLSWLPDSTLFVADGYNGTRVAKFDKDGKFLMDW
                     GMKGTPPNDTRPYYMNNVHGVALDPQTKHVFVNDRGNHRVQVFDENGKYLYEWSMGKD
                     PSDVHLLYIGADRMLWAYDRGTSKMLKYDLQGHFLYSWGTWGDFPGGFWGVHGFSVDQ
                     DGNFYTAEVDNGRAQKFIPRAGANPNYLVSKPLYSAWK"
     gene            3142..>3931
                     /locus_tag="VG272_05050"
     CDS             3142..>3931
                     /locus_tag="VG272_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWF13082.1"
                     /translation="MSELEGKVGEEIVAGNGLINRRTLLRGALLSAGVAAAGAGIADA
                     QDSVGADAPEWMKTPGRAFSGYGTPSKWQEKVQRIYTIAPGRAGTGVSRTPLHLLEGT
                     MTPNGLHFERHHNGVPEIDPNRHELFIHGMVKRPLRFSLDELMRYPMETRVHFVECSG
                     NSGIFSSPQAQQVTAGELNGLLSCSEWTGVKLSTLLADAGVNKEAKWVIAEGGDAAAM
                     SRSIPLEKCMDDAMIAMYQNGEAIRPEQGFPMRLLLPGFEGNMNV"
BASE COUNT          891 a          959 c         1185 g          896 t
ORIGIN      
        1 cttcgttatc gttaaataaa tgtttaaaaa aaaggcggca ggaatcggtg cgattcttgc
       61 cgccctttga tatcatttct ctcagcgcgt tttcaagccg tgccgctggc agaaaacggt
      121 tacttttcga agttcacgaa gtctacgttg tccgtgcagg ccaattccat gatgtcccat
      181 cctggtttca gctggaaatt caactgagtc ttccatggct tcaccaaggc tttgggatct
      241 tcgatggtga tgtcgtcgac catgttgtcg ccgtcggttt tatggaagcg ctcgacgatg
      301 tgcaactgat cgctgtgcgg gtggccgtcg cgatccagcc atgtccgttc gttgaagccg
      361 accgtatcca ctacgagagt gtcgccatcc catttgccga tggaatgtcc catgtacgtg
      421 ggcgtaatgt cttccggatg cgggcggccg tcgaggaaaa tctggcgtat ggtgtgatcg
      481 tattcaaaaa tcatgatcat ctctttggga gtcatcacga tttggaacgg gaacggctgc
      541 aaataaattc ttggagttcc aggaggcagg cagagcttta gaaccggatc atcggtttcc
      601 gtgagcgaat gggtccgcgg accgttggat ggtttgtagc tcatgtactt ctcgtttccc
      661 catggcgtca tgtccggggg ctccgcagtg tacgtgctgc cgaggaattt acgctgcgcg
      721 ggaagcacgt gcatgttcca gacgccggaa aaatcacgcg gcgatgtggg ggcggacgca
      781 gttgcagaag cgccagcaga cttagccgct ccggattgcg gcgcagcttg tgcgtgcaaa
      841 acgcaggaaa gcgaaacggt tgcgagcccc actaaagcga tcgtgccaat acgattccac
      901 attctcaaat tctcccttgg agcaagtgcg agttttgctc ggaatgattt ggccgtgatc
      961 tatagtcggc tagttcccgc cgcccgtttg caattccgtg ccatccgcgg ttacgacttt
     1021 ctgaatccag atttcgtgcg aaccgctctt ggtgggaaag ccagagatgg taacggcgtc
     1081 gccggccttc agcgtgctct tgctccagcc cacccggctc aagcgattgg ggctggtaag
     1141 ctcaccttgc catttctgcg cggcgcccgc atcgtctttg gcatcccaaa aaatttggac
     1201 gtgcggattc acgaattcaa aacccgtaac ggtgccttta actgtaattt gccggcccgt
     1261 atcatagttc gccgtgccat ggtgcgcgaa ggccattggc gcaagcgcaa gtagtccgat
     1321 gcatagagca agaatgctta cccgacaatt cctcatgttt tctccctaag aatttcaatc
     1381 cgcgaattct cgcgacattt ttgccggaga acaccctcgc gcccccgaca ctatatctat
     1441 aagacgaaat ataaggcaag aatgtttcat gagaattcta caattttaag cggacgatta
     1501 acagcattag gccagttaga gcaggatgtg attgacagca caatgccgcc gtcatataag
     1561 gctgatggca tgctgtggtc tgttgcattc caaatacaaa actgtttgta aaagcttggg
     1621 ggaaggggag acatgcgcaa agttctattg acggtgctca cgataggagt gggatgcgtg
     1681 atcggatttt cgtttgggag taacccgtgg acggttcagg ggcaaggcag cggcagtatt
     1741 gcggctgtgg ccggacaagt gggcgggcaa gacacgtttg gcgcttatga cattgacaaa
     1801 aactggcctg caaatatcag tacggtaccg ggaaatgacg cttggacttg gggagcaggg
     1861 cagggtattt atgcggagaa tccagaccgc gtgtttttgc tgtttcgcgg tgaattgcca
     1921 aatatcaagc ggccagccgc gaaattgatt ccggaatttg gacccagctt aattttcccg
     1981 atcgggcgtt tgccatggcg cgatgcaacg atttccgcat tacccggcgc aggtggaact
     2041 ggacaagatc cggatgatgg accgaagctg tggatggaag gcaaaggcgt ggttggagtg
     2101 gatgcgaagt gggaacactg cctgacgatc gtcgattcca agggaaacat tgttgaaacc
     2161 tttccgcagt acgatcacct cttcaagcga ccgcactatg tgacgatcaa tccgtacgat
     2221 ccggaaaagc acatctggat cgtggacgat cacatgcacg cgatttacat tttcacacat
     2281 gacatgaaga cgctcgtgaa gacgatgggc acgccaaccg tgaagggcgc ggacggcacg
     2341 cacttcaacc ggcccacgtt tctttcctgg ctgccggaca gcacgctgtt tgtggcggac
     2401 ggctacaacg gaacgcgcgt cgcgaagttc gacaaggacg gaaaattcct gatggattgg
     2461 ggcatgaagg gcacgccgcc gaatgacacg cgtccttact atatgaataa tgtacacggc
     2521 gttgcgctcg atccgcagac caagcacgtg ttcgtgaacg accgcggcaa tcaccgcgtg
     2581 caggtattcg atgaaaacgg aaaatacctg tacgaatgga gcatgggcaa ggatccgtcc
     2641 gatgtgcacc tgctatacat tggcgcggac agaatgttgt gggcctatga tcgcggcacg
     2701 tcgaagatgc tgaagtacga tctccaggga catttcctct actcgtgggg cacgtgggga
     2761 gatttcccgg gcggcttctg gggagtgcac ggattcagcg tggaccagga cggcaatttc
     2821 tatacggccg aagtggacaa cgggcgcgcg cagaaattta ttccgcgggc cggggcgaat
     2881 ccgaattatc tcgtcagcaa gccgctgtac tcggcctgga aataaatttt ttgtgcggga
     2941 gttgttcgtg gcgcgcttga aatttattta gactcaatct aaacccgcac cctttgctca
     3001 gaaaacgcaa agggtgcggc accccggtcg tgagaaatgc ataaatggaa gcttgaggcc
     3061 gcctcgatgt cacggtaagc tttccctgac attgaggcgg cttttttgtg gcgtatcgcg
     3121 agacaagttt tgggggcaaa cgtgagcgag cttgagggca aagtgggtga agaaattgtg
     3181 gcggggaatg gattgatcaa tcggcgcaca ctgttgcgtg gtgcgctctt gtccgccggg
     3241 gtcgccgctg cgggcgcggg gattgcagat gcgcaggatt ctgttggagc ggatgcgccg
     3301 gagtggatga agacgccggg gcgcgctttt tctgggtacg gcacaccctc gaagtggcag
     3361 gaaaaagtgc agcgcattta cacgatcgcg ccggggcgcg cggggactgg cgtttcgcgc
     3421 acgccgttgc atttgctgga aggaacgatg acgccgaatg gcttgcactt cgagcggcac
     3481 cacaatggag ttccagaaat tgatccgaac cggcacgaat tatttattca cggcatggtg
     3541 aagcgaccgc tgcggttttc gctcgacgaa ctgatgcgtt atccgatgga gacgcgggtt
     3601 cattttgtgg agtgttcggg aaacagtgga attttcagca gcccgcaagc gcagcaagtg
     3661 acggccgggg aattgaatgg attgctttcg tgctccgaat ggaccggcgt aaaactttcg
     3721 actttgctcg cggatgcggg cgtaaataag gaagcgaagt gggtgatcgc cgaaggcggc
     3781 gacgccgcgg cgatgagcag aagcattccg ctggaaaaat gtatggacga cgcgatgatc
     3841 gcgatgtacc agaacggcga agcgattcgg cccgagcagg gatttccgat gcgtctgctg
     3901 ctgccgggct tcgagggcaa catgaatgtg a
//
LOCUS       DASZMB010000111        21094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2309358, whole genome shotgun sequence.
ACCESSION   DASZMB010000111 DASZMB010000000
VERSION     DASZMB010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 21094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21094
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2309358"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VG272_05055"
     CDS             <1..309
                     /locus_tag="VG272_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13083.1"
                     /translation="FRINLKQCVWVAAMGLAFGISGGATRLVATPTPQEHHDEDYSKN
                     KNYQTGMRDGRDDQSRNRDHYKKRHFKKDDDRNAYESGYQAGHGEKHDDHEEHRDQKY
                     "
     gene            633..1133
                     /gene="coaD"
                     /locus_tag="VG272_05060"
     CDS             633..1133
                     /gene="coaD"
                     /locus_tag="VG272_05060"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HWF13084.1"
                     /translation="MQTGETMKNVLAIYPGSFDPVTNGHLDLIERGAKIFDRLVVSIL
                     RNFEKEPLFTVEERVEMLREVTRAWPNVEVDVFSGLLVDYARLRQANVLLRGIRAVSD
                     YEYELQMALMNRKLNPQVETVFMMPSVKYSYLSSRLVREIAQLGGSITDLVPPVVEER
                     LRAKIL"
     gene            1168..2352
                     /locus_tag="VG272_05065"
     CDS             1168..2352
                     /locus_tag="VG272_05065"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWF13085.1"
                     /translation="MQLAARVNRISISPTASVLAAAEKLRAQGVDLADFGPGEPDFPT
                     PEHIQQAAIRALKEGRTKYTPVGGIGPLRDAICKWHTAQFGSSYTVPETIVTVGGKHA
                     IFNTFCSLLEKGDEVLIPAPYWVSYPDMVLYTGATPVPVATDPSDGFRLRAEQVARAI
                     TPRTKLLIVNSPSNPSGAVVPEDEFVKIYEVCRAKNIWVMTDECYSHFVYGKEKPYSL
                     ASVPGTKPNIIVAGSLSKTFAMTGWRIGYALAPEPLISAMIKTQSQSTSNPTSIAQYA
                     ALEALTGPMDTVTAMLAEYSRRRDRIVSGLRAIPGVTCVEPGGAFYAYPSIAAHLGNG
                     IADSTALAKVLLEKQHLVCVPGDAFGTPGYIRLSYATSIDRIEEGLRRLEKFFAMAET
                     AR"
     gene            2349..3419
                     /locus_tag="VG272_05070"
     CDS             2349..3419
                     /locus_tag="VG272_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HWF13086.1"
                     /translation="MKSESNLMGRAPARLAPIFVPRIWGTHDLSPLFPEHSQEIEPIG
                     EVWLTGNECKFASGEFAGRALGEVWPSLPVEWTGTRLQGLPRMPLLVKFIFPEDKLSV
                     QVHPDDAYAQQHEAAAGGAGKTEMWYVVSARAGAGVRVGLDPAVTRESFQAAVARGTA
                     EECIGSVAVRAGDAVFVPAGITHTICPGVVLCEVQQQSDVTYRVFDYNRVGADGKPRA
                     LHVERALDVMRFGEQRGGLCDPVRVTHGAATETFYAACRYFATERWEFRERITTATSR
                     EHFDLLIFLAGEGRIEYEGGVETFGPAQVWLLPAALGDYHVTPNSPTTLLRAYVPDLN
                     NLIRRFSEEHIPKEKWARVVHL"
     gene            3416..4552
                     /locus_tag="VG272_05075"
     CDS             3416..4552
                     /locus_tag="VG272_05075"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HWF13087.1"
                     /translation="MKKTRSDEGLSAHAIILAGGRGTRFWPRSRTRTPKQLLNIVGSA
                     TMLEQTAARLGPLFPPSHQWVVTNTEQVAAVRKQMPRVPASHILAEPVGRNTAAAIGL
                     SAAHLLHSGMGKENDALMAVLPADHYIAKPAAYLKIVSAALRVARSAGSLVVLGIPPS
                     RPETGYGYIERSRAKTVSAEGVPVFTVQQFTEKPELPRAKKYVASGRYYWNAGMFFWR
                     VSTFLGNLEKFLPKTHDALMRLAGEIDTPRYERTLARIYPQLENISVDYAILEQVTHR
                     SAAPSVFVLPAEVGWSDIGSWAAVYELLARKAGENVSAGKFAALDAQGNFFWSPKKLV
                     AAVGIRDLIVVETEDALLLCPRDRAQEVGKIVKWLEAEQLSRLL"
     gene            4762..6171
                     /locus_tag="VG272_05080"
     CDS             4762..6171
                     /locus_tag="VG272_05080"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HWF13088.1"
                     /translation="MSTIKFGTDGWRGVIADDFTFENVRKVAFAIARYVVRAEKPGAG
                     VLIGYDTRYGSERFARTTAEVVSITGMPVWIAEKACPSPALSLLVRQRGAAGGIMITA
                     SHNPYKWNGVKFKASYGSSALPSIVEQVEKELATVLANGIPPLPPQTENIQSLDILSP
                     YLEAIEKIVDWDRIREANFRFVVDPMHGAARGLLREVMTRHGVACDEIRGTRDPLFGG
                     VNPEPIEPHIEALRKAVLAGKYDAGLCADGDGDRIGAMDRNGIFITPHQIFSILLWHL
                     AGTRGIPGDVAKTFSSTKMLDKIAGKYGRKIFETPIGFKYICELMLERDILLGGEESG
                     GIGTKLYLPERDATVMALLLVEVMAWHKKTLGELVAQLHEEFGEHHYGRVDLELKAGQ
                     KERAIEHFSGANVKRVLDWEVARRENLDGIKLYLEGIGWVMLRASGTEPMLRVYSETR
                     RAKDTQKILEHTCSVVRGL"
     gene            complement(6277..6711)
                     /gene="nusB"
                     /locus_tag="VG272_05085"
     CDS             complement(6277..6711)
                     /gene="nusB"
                     /locus_tag="VG272_05085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HWF13089.1"
                     /translation="MSLRRKSREYALQMLFQWDMGKQELSKIEDGFWKNAKAQKSTRE
                     FANQLFESAAARTGEIDPIISLHAQNWRIERMSAIDRAILRLALCEIRTTSTPAKVVI
                     NEALELAKKFSTEEASPFINGILDAAAKSSAEKPSSSPPAEN"
     gene            complement(6712..7245)
                     /gene="ribH"
                     /locus_tag="VG272_05090"
     CDS             complement(6712..7245)
                     /gene="ribH"
                     /locus_tag="VG272_05090"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HWF13090.1"
                     /translation="MEKNFHGKEIRGAASAVGLRFALVVSRFNSFITDRLLAGAVDAL
                     EAAGADPENIVVVRVPGSFEIPLTAKKLAAGGRVDAVIAIGCILRGETAHFDYVASEV
                     ARGVQLAQLDTGIPVIFCVLTCDTLEQAIDRAGLKSGNKGRDAGLAAIEMANLSRQFR
                     PAEVSRKAKTTSRGKHS"
     gene            complement(7273..8055)
                     /locus_tag="VG272_05095"
     CDS             complement(7273..8055)
                     /locus_tag="VG272_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HWF13091.1"
                     /translation="MAYENLLYEKRDRIGFVTFNRPKVLNALNHKTMEELRDILISAR
                     EDLDVRVLILTGAGEKSFVAGADISELAVQTPVSGKETAAFGQSVLNLLETLGKPSIA
                     AINGFALGGGCEVALVCSMRLASKSAKLGQPEVKLGIMPGYGGSQRLTRLCGKGVAYE
                     LCLTGEMISADEALRIGLVNHVYEPAELLPAAEALAKKIIANAPLSVKFTMEAIERGS
                     EMPQAEGLYLETALFGLCAATEDMREGTRAFLDKRAPQFQGK"
     gene            complement(8065..10632)
                     /locus_tag="VG272_05100"
     CDS             complement(8065..10632)
                     /locus_tag="VG272_05100"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWF13092.1"
                     /translation="MSVDIVKQLDRAKRLVEKRSIDEAIEAYQTVLVDFPNHLESIQS
                     LGDLYTMQNRPDRAAVYYGMLFDRFLAPREEPKALALYTRFLKSQQQPPQRVARYALL
                     LQKQNRAEEALEQFMSAALAFELSDKGEEALACFVRIAQLDSENRDRHVTVANLAERL
                     GNLTAAARGYLRAGQLTTDNDDEALEFFAKAHEIIPGDRSAALHYAQALMRKGDSTAA
                     AAILGPLSEVERDAPFLDTYADALIRSSQLDTARILLDSMQRQGIGSAEKFFLLTTEF
                     LRAGQEEKAVELLVATKKSMLAARNESEFAAAADKLVEAFPKSSRLAKFWADLYSELN
                     RETKFFEAEVHLFDLHLENNEIHEVCEAFEKLVEIDPYDSRNQERYDLLSGRADDDFL
                     KRVKSRLSNAATHNPDTAAPQPHSKHPHEPSPAILEAVQSRQTLEDLLVQAEIFIQYS
                     LQSKAVERLQRIVELFPGEDENNERLQSLFDAARWWPEKGVAPVIPAEPPKPAVEAPF
                     ARNAATSPFAPETLRDLSKISEINQNVFRQPSPRAMLSVSVNEVGNYLRVTRCLGAIG
                     PPGQPPQMASEFCSTGIQPSSSNQIVRLMSQLSVAAPDSLGGLPLDAATAPIVAEMGL
                     ETALGVQLMDPETQSPAGMMILGYSASHAWRPNETYFLQSVGDQMLMCVHHTRLRTLI
                     RTLGVADEKTGLLARSSYIDCLLHESQRARKQGMPLALALLQVDGGPDLLRQQGEGPF
                     DRYMEQLGESVHAIVRQTDLAIKYTSWALAIVLPDTPLPGARKFAEKLKAAVGGLRPP
                     WEAAPLTMSVAVAEAIAGKDYDNEDIVTDLINRVESGLEEARKRGGNEIIALELAKN"
     gene            11163..12971
                     /gene="lepA"
                     /locus_tag="VG272_05105"
     CDS             11163..12971
                     /gene="lepA"
                     /locus_tag="VG272_05105"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HWF13093.1"
                     /translation="MDPRFVRNFCIIAHIDHGKSTLADRLLELTGALSRREMSAQVLD
                     SMDLERERGITIKAKSIRLHYSAKDGNEYQLNLIDTPGHVDFSYEVSRALSACEGALL
                     VVDASQGVEAQTVANTFLAAHHNLTIIPVINKIDLPAAQPDQAKEQIENVLAIPAADA
                     LLISAKSGIGVESVLEAIVARIPPPTGSAEAPLQALIFDSWFDPFRGAVVLVRVMNGR
                     MTKHMKIRLCFNDQVYEVEALGALTPKPVVLEELVAGDVGFVIANIKQVADARMGDTV
                     TEAARPAPPLPGFEATKPMVFAGLFPVLATDYENLRDALGKLQLNDAAFFYEPENSVA
                     LGFGFRCGFLGLLHMEIAQERLEREFNINLITTAPSVRYRITTTAGDIVEVDSPTKFP
                     PPNEMAKIEEPVITAMIITAEDSVGPILQLCEEKRGVQKNFEYLSPQRVMLTYELPLN
                     EIVLDFYDRLKSASRGYASLDYHLAGYKEADLVKLDVLIGGEPVDALALVCHREFSYD
                     RGRDLVSRLRKLIPRQMFEVPIQAAIGSRVIARETVAAIRKNVIAKCYGGDISRKRKL
                     LEKQKEGKKRMKRVGRVDIPQEAFLAVLKVASTSDE"
     gene            complement(13171..14553)
                     /gene="xseA"
                     /locus_tag="VG272_05110"
     CDS             complement(13171..14553)
                     /gene="xseA"
                     /locus_tag="VG272_05110"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HWF13094.1"
                     /translation="MAEKVKQFELNLAPERKIWGVGELTSRISGVLSSQFTNLWVEGE
                     VSNCRPAQSGHIYFTLKDAKAQVKCVCFRTQAMRLKCRPEDGLKLIVRGSISVYEPRG
                     EYQIYVEHIEPSGIGALQLAFEQLKKRLAAEGLFDQSRKKPLPLLPARVGVISSPTGA
                     AVRDIIRILRRRFPNLHLILHPVRVQGDGAAEEIANALKYFNRKQTVDVILLARGGGS
                     IEDLWAFNEEVVARAMASCSIPIISGVGHETDTTIADFVADVRASTPSAAAEIVVKSR
                     QEFQRHLSELESKISQRMRYVLLEHRHHLKELTTHMGFRRLEDLLRQHRQQTDELTMR
                     LGDALRGRLEVLRHRHTIAATRISSFDLRARIRALGLRLMQRSAELGVRLERVLGGKI
                     QMLERLRVQLDERGPLRVLQRGYSICTDAAGNVVNSAEQVAVGAEVNVQLAFGRLGAE
                     VRTRDIPKPQ"
     gene            complement(14538..14771)
                     /locus_tag="VG272_05115"
     CDS             complement(14538..14771)
                     /locus_tag="VG272_05115"
                     /inference="COORDINATES: protein motif:HMM:NF022596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HWF13095.1"
                     /translation="MRELGRMMVFAGAVLAVLGALVWLAARPGGLPLRLGHLPGDIAY
                     QGKHGSFYFPVVTCILLSLVLTLVMWLVQWLKR"
     gene            complement(15042..15461)
                     /gene="ndk"
                     /locus_tag="VG272_05120"
     CDS             complement(15042..15461)
                     /gene="ndk"
                     /locus_tag="VG272_05120"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: protein motif:HMM:NF001908.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HWF13096.1"
                     /translation="MASERTLAIIKPDAFGRGLAAEIVGRINMAGFQIVAIKSMRLTK
                     KEAEGFYAVHAARPFFGELTEFMSSGKVVVLALEAEGAILKWRDTMGATDPAKAAPGT
                     IRRDLGTSIGNNCTHGSDAPETAAFELGYFFAGHELI"
     gene            complement(15573..16445)
                     /gene="sucD"
                     /locus_tag="VG272_05125"
     CDS             complement(15573..16445)
                     /gene="sucD"
                     /locus_tag="VG272_05125"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF004230.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HWF13097.1"
                     /translation="MSVLVNENTRVVVQGLTGREGSFHAGQMIEYGSKVVAGVTPGKG
                     GTKHESVPMFNTVADAVRETGADASIIFVPPPYAADAVLEAIDAGLPLVVCITEGIPT
                     LDMVRVAAALKLSKTRLIGPNCPGIISPGKCKMGIMPGRIHKQGNIGVVSRSGTLTYE
                     AVHQLTQLGIGQSTCIGIGGDPIIGTTHIDAIKLFNEDSETQAIVMIGEIGGDAEEKA
                     AAYIKDNVKKPVVGLIAGQTAPPGRRMGHAGAIISGGAGTAAEKYKAMAAAGILTIQS
                     PADIGVTLARAIKK"
     gene            complement(16504..16884)
                     /locus_tag="VG272_05130"
     CDS             complement(16504..16884)
                     /locus_tag="VG272_05130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HWF13098.1"
                     /translation="MPERAGVVRTYSDLLVYKQSYRLALDVSNVTKAFPKQEQFELGR
                     QIRNCSRSVAANIVEGWAKRNSAAEFKRHLIIAIGECAETKFWIDLATDEGFVEPKRA
                     GTLGSEYSKLGFMLHNLWKEWRKF"
     gene            complement(16926..18092)
                     /gene="sucC"
                     /locus_tag="VG272_05135"
     CDS             complement(16926..18092)
                     /gene="sucC"
                     /locus_tag="VG272_05135"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HWF13099.1"
                     /translation="MKIHEYQAKEIFRKFGVPTPRGEMVTTPDQARAVAERLGTKIVV
                     VKAQIHAGGRGKAGGVKLAKSPAEAADLAKSIMGMTLVTPQTGPAGRVVQRLLVEEGL
                     DIKRELYLSILVDRASAAPVIMASTAGGMEIEEVAHTNPELILRETILPATGLQPYHT
                     RKIAYGLGLSGDAAAAVGPFLQALYRAFLETDASLVEINPLIVTGDGKLVALDAKMAF
                     DDNALYRHKDLADLRDLNEEDPLEVEASTYKLNYIKLDGNVGCMVNGAGLAMSTMDII
                     KLSGGSPANFLDVGGGASAEQVKNAFKILISDKSVKGVFINIFGGILRCDVLANGVVA
                     AAKELNLKLPVVIRMEGTNVEKGQEILRDSGCNFTIAKDMKDGADKIVALAGKG"
     gene            18349..19368
                     /gene="trpD"
                     /locus_tag="VG272_05140"
     CDS             18349..19368
                     /gene="trpD"
                     /locus_tag="VG272_05140"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HWF13100.1"
                     /translation="MMRSLIQKVEAGADLSRQEAENAMEEILSGRANEETIVALLSSL
                     RAKGETVGELVGFARAMRRQVVSVFANGMRPDEMLVDTCGTGGDSSGTFNISTAAAFV
                     AAGASVHVAKHGNRSISSKCGSADVLEALGVSLDIPPERVGAAIHEIGIGFLYAPAMH
                     TAMKHAMPARRKLGRTAFNLLGPLTNPAGARAQVVGVFSDKIVEKVALTLSELDVERA
                     FVVHGDGGLDEISLAGDTAVGDVHRGVVSLYRITPEHFGLERAPLESISGGDAAYNAE
                     IIRAIFSGERGPRRDIVVANAAAAIVASGRATDFLEGAQLAAASIDSGEARKKLEALI
                     AFTHR"
     gene            complement(19452..20012)
                     /locus_tag="VG272_05145"
     CDS             complement(19452..20012)
                     /locus_tag="VG272_05145"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HWF13101.1"
                     /translation="MILLLDNYDSFTYNLAQFLGQLGEKLEVHRNDKLTLDQIAATKP
                     QRIVISPGPGTPQEAGICIDVIKRFAGEIPILGVCLGHQAIGHAFGGNVIRAPEIMHG
                     KTSPIHHDNKTIFEDLPQDFVATRYHSLIVEEKSFPADLEISAKTDGIIMGLRHKKMC
                     VEGVQFHPESVLTEVGMQLLKNFLSL"
     gene            complement(20226..20825)
                     /locus_tag="VG272_05150"
     CDS             complement(20226..20825)
                     /locus_tag="VG272_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13102.1"
                     /translation="MRAHSIRAVVFSTLLLGLFCVRTARAQQQEQPEQQDQPHQRGPS
                     TVEERARAVKVAHELENEPLAKDAKDNRDWVIQWIVDIPDITVTVCDDYFGTLSKPVR
                     GHVREIVNQMVISSAAYMIEHPDKLKDEQAIATAGLLGSLKTYEAILKQEPDARWPYI
                     DKIVMMRDQGKLDDFVSDTRRKCAQEEEEPDPDTIRAAR"
BASE COUNT         4661 a         5851 c         5758 g         4824 t
ORIGIN      
        1 ttccggataa atttgaagca gtgcgtatgg gtagctgcaa tgggcttggc attcgggatt
       61 tccgggggcg caacgcggct tgtggcgacc ccaactccac aggagcacca cgacgaagat
      121 tattcgaaaa acaagaatta tcaaaccggt atgcgggacg gacgcgatga ccaatcacgt
      181 aaccgcgatc attacaagaa gcgtcacttc aagaaagatg atgatcgcaa tgcatacgaa
      241 tccggatacc aggccggcca cggtgaaaag catgacgatc atgaagaaca tcgagaccaa
      301 aagtattgag cggatggatc cggcagtccg cggacgtttt ccgttcatcc tacaagagca
      361 aaattcttaa agatacgatt tgaagtttat tcagccgctc tttgaaacgt cagccagcac
      421 attgcatccg caaggagagc ataaaagact tcccgcgatg cgctggctga cctatccgct
      481 ccaaaccgcg caaggcggcc aacttcctca tttctaatag tttgcctgtt aaattcccgc
      541 acaatcaata gctttgtcct ttagccaggc acgcagcaat tcctatgcgt aatcttcgga
      601 aattttcgtt acaatagcga ggaaccgaaa gtatgcagac gggcgaaacc atgaaaaatg
      661 tcctggccat ttatcctgga tcgtttgatc ctgtgacgaa cgggcatctt gacctgatcg
      721 agcgtggcgc aaagatattt gaccgtctgg tggtcagcat tttgcgcaac ttcgagaagg
      781 agccgctgtt tacggtggaa gagcgcgtgg agatgttgcg cgaggtgacg cgtgcctggc
      841 ctaatgttga ggtcgatgtt ttcagcggat tgctcgtcga ctacgcgcgg ttgcgccagg
      901 ccaatgtttt gttgcgcggc atacgcgcgg tttccgatta cgagtacgaa ttgcagatgg
      961 ccctgatgaa tcgtaaactg aatccgcagg tcgaaacggt ttttatgatg ccctcggtta
     1021 aatatagcta tctgagttcc cggctggtgc gggaaatcgc gcagttgggc gggtcgatta
     1081 cggacctcgt gccgccggtg gtggaagagc gcctgcgcgc taaaattttg taatcttacg
     1141 aagttacctt tttaaggaga atcatccgtg caactcgctg cccgcgttaa tcgtatttcc
     1201 atttcgccga ccgcttccgt tcttgcggcg gccgaaaaac ttcgcgccca gggcgtcgac
     1261 ctcgccgact tcggaccggg cgagccggac ttcccgacgc cggaacatat tcagcaggca
     1321 gcgattcgcg ccttaaagga agggcgaacg aaatacacgc cggtaggcgg cattggaccg
     1381 ctgcgcgatg cgatttgcaa atggcatacg gcgcaattcg ggtcgtccta cactgtgccg
     1441 gagacgattg tcacggtcgg cggcaagcac gccattttta acacgttctg ctcgttgctc
     1501 gaaaaaggcg acgaagttct aattcccgca ccgtattggg ttagctaccc ggacatggtt
     1561 ttatacacag gcgcaacgcc tgtgccagtg gcgaccgatc catccgacgg cttccgcctg
     1621 cgtgccgagc aagttgctcg cgccatcacg ccgcgcacca agttgctgat cgtcaactcg
     1681 ccaagcaacc cgagcggcgc cgtagtgccg gaagatgaat tcgtaaaaat ctacgaagtc
     1741 tgcagggcga aaaatatttg ggtgatgacc gacgaatgct actcgcactt cgtgtacgga
     1801 aaagagaagc cgtattcgct ggcgagcgtt ccgggcacga aaccaaacat cattgttgcc
     1861 gggtcactgt caaaaacatt tgcgatgacc ggctggcgca ttgggtatgc ccttgcacct
     1921 gagccgctga tctcggcgat gatcaagacg caaagccaat ccacgtcgaa tccgacttcg
     1981 atcgcgcaat atgcggcgct cgaagcgctg actgggccga tggatacggt gaccgcaatg
     2041 cttgctgaat attcgcgtcg ccgcgaccgc atcgtttccg ggctgcgcgc aattcccggc
     2101 gtcacctgcg tggaaccggg aggcgcgttc tatgcttacc caagcatcgc cgcgcatctg
     2161 ggcaatggca ttgcggactc gactgcgctc gcgaaagtgc tcctcgagaa gcagcacctg
     2221 gtttgcgttc cgggagatgc gtttggcacg ccgggctata tccggctttc gtatgccact
     2281 tccattgacc gcatcgagga aggtttgcgg cgcctggaaa aattctttgc catggcggaa
     2341 acggccaggt gaagtcggaa tcaaacctga tggggcgggc gcctgcgcgg ctcgccccaa
     2401 tttttgtgcc tcgcatctgg ggcacgcacg acctctctcc gctgtttccc gaacactccc
     2461 aagaaattga gccgatcggc gaagtgtggc tgacgggcaa tgaatgcaag tttgcttccg
     2521 gcgaatttgc cgggcgcgcg ttgggcgaag tgtggccttc ccttccagtc gaatggactg
     2581 gaacacgctt gcagggactg cctcgcatgc cgctacttgt gaaatttatt ttccctgaag
     2641 acaaactttc cgtgcaggta catccggatg acgcgtacgc gcagcaacat gaggcggcag
     2701 cgggcggcgc cgggaaaacg gaaatgtggt atgtggtgag cgcacgcgcg ggggcaggcg
     2761 tgcgcgttgg acttgatcct gcggtgacac gcgaatcatt tcaagctgcc gttgcccggg
     2821 gcacggcgga agaatgcatt ggaagcgttg cagtgcgtgc gggcgacgct gtttttgttc
     2881 cagctgggat aacgcacacg atttgtcccg gcgtggtgct gtgtgaggtt cagcagcaat
     2941 cggacgttac ctatcgcgtc ttcgattaca accgagttgg tgctgacgga aagccgcgcg
     3001 cgctacacgt tgagcgagcg ctggacgtca tgcgatttgg cgagcagcga ggtggactgt
     3061 gcgatccagt gcgcgtcact cacggcgcgg ctactgaaac tttttatgcg gcttgccgat
     3121 actttgcgac ggagcgctgg gaatttcgcg agcgcatcac aaccgctact tcacgggaac
     3181 attttgactt gctcattttt cttgcgggtg aagggcgcat cgaatatgag ggtggcgtgg
     3241 aaactttcgg tcccgcacaa gtctggctgt tgcccgccgc gctcggcgac tatcacgtta
     3301 caccaaattc tcctaccacg cttttgcgcg cgtacgtgcc tgacttgaat aatttaatcc
     3361 gacgatttag cgaggaacac attcccaagg aaaaatgggc gcgcgtggtg catctatgaa
     3421 aaaaacacgc agtgatgagg gtctttccgc gcatgccatt attctcgccg gcgggcgagg
     3481 cacgcgcttc tggccgcgca gtcgcacgcg aacgccgaaa caattgctca atattgttgg
     3541 cagcgccacg atgctggagc agactgctgc gcgccttgga cctttgtttc cgccatccca
     3601 tcagtgggtg gtgaccaaca cggaacaagt cgcggccgtg cgcaagcaaa tgccacgcgt
     3661 gcccgcgtcg cacattctcg ccgagcctgt aggacgaaac acggccgctg cgatcggact
     3721 ctctgccgcg cacttgctgc acagcggcat gggcaaggaa aacgatgcac tgatggccgt
     3781 gcttcccgcg gaccattaca ttgcgaaacc tgcggcctat ttaaaaattg tgagcgccgc
     3841 gctgcgtgtc gcgcgctctg ccggatccct cgtcgtactg ggaattccac cctcgcggcc
     3901 agaaacagga tacggatata tcgaacgctc gcgcgctaaa acggtgagcg cggaaggggt
     3961 tcccgtattc acggtacaac agttcacgga aaaaccggaa ttaccgcgcg cgaaaaagta
     4021 tgtggcttcc ggacggtatt actggaatgc aggaatgttt ttctggcgcg tctccacatt
     4081 tctaggcaac ctcgagaaat ttttgcccaa gacgcatgac gcgctgatgc gcctagccgg
     4141 ggagatcgac acgccacgtt acgagcgcac gctcgctcgt atttatccgc agctcgaaaa
     4201 catttctgtc gactatgcaa ttctggaaca agtcacgcac cgctccgctg cgcccagcgt
     4261 tttcgtgctg ccggcggaag tgggctggag cgatatcggg tcctgggctg ccgtctacga
     4321 actgctcgcg cgtaaagctg gagaaaatgt ttccgccggt aaatttgcgg cgcttgacgc
     4381 gcaaggaaat tttttctgga gcccgaagaa actggttgcg gcagtcggga ttcgagatct
     4441 gattgttgtg gagaccgaag acgcgctgct gctctgccct cgcgaccgcg cgcaggaagt
     4501 cggcaaaatt gtgaagtggc tggaagcgga acagctttcc cgactgcttt agctccactt
     4561 tcatcaagta attaaacctg tttacgtgcc ataggcttca gcctgaaggg gttgagcgct
     4621 tgcaaggcca caaccccaca ggctgaagcc tatgccactt cccgcactca tctcccggac
     4681 gccccctctt ttgtgtgaat cgcaatgcag tcttacggag ctgcttctcg cccactcggg
     4741 cttcgcatat acttttgaac tatgagtacg atcaagtttg ggacggatgg ctggcgcggc
     4801 gttattgccg acgattttac gtttgagaat gtgcgcaagg tcgcgttcgc gatcgcgcgg
     4861 tacgtggtgc gcgcggaaaa acctggagcg ggcgtgctca tcggctatga cacacgctac
     4921 ggctcggaac gctttgcgcg cacgacggcc gaagtcgttt ccataactgg aatgcctgtg
     4981 tggatcgcgg aaaaagcttg cccatctcct gcgctttcac tgctggtaag gcagcgcggg
     5041 gcggcaggcg gcatcatgat tacggcgagc cacaatccgt acaaatggaa tggcgtgaaa
     5101 ttcaaggcga gctacggcag ttcggcgctg ccctcgattg tggagcaagt ggaaaaagag
     5161 ctggccacgg tgctggcgaa tggcatcccg ccgctgcccc cgcaaacgga gaacatacaa
     5221 tcgctggata ttctttctcc gtatctggaa gccattgaaa aaattgtgga ctgggaccgg
     5281 attcgcgagg cgaactttcg ttttgtggtt gatccaatgc atggcgcggc gcgcgggctt
     5341 ttgcgcgaag ttatgacccg ccacggcgtg gcatgcgatg aaattcgcgg cacacgcgat
     5401 ccgctgtttg gcggcgtgaa tccggagccg atcgaaccgc acatcgaggc gctgcgaaag
     5461 gcggtcctcg cgggaaaata tgatgcggga ctttgcgcgg acggagatgg cgaccgtatc
     5521 ggcgcgatgg atcgcaacgg catatttatt acaccgcatc agattttttc cattttgctg
     5581 tggcatctcg cgggcacccg cggtattccc ggcgatgtgg cgaaaacttt ttcttccaca
     5641 aaaatgctgg acaagatcgc tggaaaatac ggccgcaaaa tattcgagac gcccatcgga
     5701 tttaaataca tctgcgaatt gatgctggaa cgcgacatcc tgctgggggg agaagaatcc
     5761 ggcggcatag ggaccaaact ttatttgccg gagcgcgatg cgacagtcat ggctttgttg
     5821 ctggtggaag tgatggcctg gcacaagaaa actctcggcg aactcgtcgc gcagttgcac
     5881 gaggaattcg gcgaacatca ttacgggcgc gtggatctgg aactcaaggc agggcaaaaa
     5941 gagcgggcga ttgagcattt cagcggggcg aatgtgaagc gcgtgctcga ctgggaagtc
     6001 gcccgacgcg aaaatttaga tgggattaag ctctatcttg aggggatcgg ttgggtgatg
     6061 ttgcgtgcat cggggacgga gcccatgttg cgcgtgtatt cggaaacgcg aagggcgaag
     6121 gacacgcaga aaattctgga acatacgtgc tccgttgttc gcgggttgta ggggcggttt
     6181 gcgattctct gcggaacggc ctcgcgtccg tcctctcagg gcaaaagagg cgggctgcag
     6241 caggcggtca cgcagaatac gctacactgt ttggaattaa ttttccgccg gaggcgagga
     6301 cgacggtttt tcggctgaag attttgctgc ggcgtcgagg ataccgttga tgaacggaga
     6361 tgcttcttct gttgaaaact tttttgccaa ttctaaggcc tcgttgatta ctactttcgc
     6421 cggcgtgctc gtcgtgcgga tttcgcagag agccaggcga aggattgcgc ggtcgatcgc
     6481 cgacatgcgc tcgatgcgcc aattttgagc gtgcaaagaa atgattggat cgatttcccc
     6541 ggtgcgagcc gcggcacttt caaaaagctg attcgcaaat tcccgggtgg atttttgagc
     6601 cttcgcattt ttccaaaaac cgtcttcaat ttttgagagt tcctgttttc ccatgtccca
     6661 ttgaaaaagc atttgcagcg cgtactcgcg tgattttctg cgcagcgaca tttaggagtg
     6721 cttcccgcgg gaagttgtct tggccttacg cgaaacttcc gcaggacgaa actgcctgga
     6781 gagatttgcc atttcgattg cggcgagacc cgcatcgcgg cctttgtttc cgctcttgag
     6841 cccggcgcga tcgatggctt gctcgagcgt gtcacaggtg agcacgcaaa aaattactgg
     6901 aatgccagtg tcgagctggg cgagttgcac gccgcgcgcc acttcgctcg ccacataatc
     6961 gaaatgcgct gtttcgccac gcagaatgca accgatggcg atcacggcat ccacgcgtcc
     7021 gccggcagcc aacttttttg cggtcagcgg aatttcaaat gaaccgggaa cgcgaacgac
     7081 gacaatattt tccgggtcgg ctccggcagc ttcgagcgcg tcgactgctc cggccaacag
     7141 gcgatcggta atgaaactat tgaagcggct gacgaccagc gcgaaacgca agcccacggc
     7201 actggccgct ccgcgaattt cttttccgtg aaagtttttt tccatgtgat tgattatttt
     7261 ttcgcgtcgt tattactttc cttgaaattg gggtgcccgc ttgtccagaa acgcgcgcgt
     7321 tccttcccgc atgtcttccg ttgccgcaca caggccaaac agcgccgttt ccagatacag
     7381 gccctcagcc tggggcattt cactgccgcg ttcgatggct tccatcgtaa actttaccga
     7441 aagcggcgca ttggcgataa tttttttcgc gagtgcttcc gctgcgggca acaattccgc
     7501 cggctcgtat acgtgattca cgagaccaat ccgcagcgct tcgtccgctg aaatcatttc
     7561 tcccgtgagg catagttcgt aggcaacgcc cttgccgcac aagcgcgtaa gtcgctgcga
     7621 tccgccgtaa cccggcatga tgccaagttt gacttccggc tgtccgagct tcgcgctttt
     7681 gctggctaat cgcatcgaac acaccagtgc gacttcgcaa ccaccgccca gcgcaaatcc
     7741 attgatggcg gctatcgaag gttttcccag cgtctccagc agattcaaaa cgctttgccc
     7801 aaacgccgcc gtttccttgc cgctcaccgg agtttgcacg gcgagttccg aaatgtccgc
     7861 tcctgcgaca aaagattttt caccggcgcc ggtgagaatc aaaacgcgaa catccagatc
     7921 ttcacgcgca gaaatcaaaa tatcgcgcaa ctcttccatg gttttgtgat tcagagcgtt
     7981 caaaactttg ggacggttga acgtgacaaa cccgatacga tcccgctttt catagagcag
     8041 gttttcgtaa gccatctaca ttcctcaatt ctttgccaat tccagggcaa taatttcgtt
     8101 gccaccgcgc ttgcgagcct cttccaggcc cgattccacg cggttaatca aatccgtcac
     8161 gatatcttcg ttatcgtaat ccttgccggc gatcgcttcg gcgactgcca cactcatcgt
     8221 taatggcgcc gcctcccagg gaggccgcaa cccgccgaca gcggctttca gtttctccgc
     8281 gaatttccgt gcgcccggca gcggcgtgtc cggcaggaca atcgcgagag cccatgaagt
     8341 gtacttgatt gcaaggtcgg tttggcgaac gattgcatgg acagactccc ccaactgctc
     8401 catgtagcgg tcaaaaggac cttccccctg ctggcgcagc aaatcgggac cgccgtccac
     8461 ctgcaataga gccaaagcca agggcatgcc ttgcttgcgc gcgcgttgag attcgtgcag
     8521 caggcaatcg atataagaac ttctggcgag caaacccgtt ttttcatcgg ccacgccaag
     8581 cgttcgaatg agtgtgcgca ggcgagtatg gtgtacgcac atgagcattt gatcaccgac
     8641 cgattgcaga aaataagttt cgttcggcct ccaggcatga gaagcagagt aaccgagaat
     8701 catcatccca gcgggagatt gcgtctcggg atccatcagt tgcactccaa gcgccgtttc
     8761 caagcccatt tccgcgacaa tcggcgcagt ggccgcgtca agtggcagcc caccaagcga
     8821 atccggagca gcgactgaca attggctcat caaacgaacg atctggtttg aggaagatgg
     8881 ttggattccc gtggaacaaa actccgaggc catttgcgga ggctgccccg gcgggccgat
     8941 cgcacccagg catctcgtaa ctcgcaaata attgcccact tcgttcaccg aaaccgaaag
     9001 catggcgcgc ggcgaaggct gccgaaaaac gttttgattg atttccgaga ttttcgaaag
     9061 atcacggagg gtttccggcg cgaagggaga agtggcggcg ttgcgagcga acggcgcctc
     9121 cacggctggc tttggcggct ccgccgggat gacaggggct acaccttttt ccggccacca
     9181 gcgcgccgcg tcgaacaggc tttgcaggcg ttcattattt tcgtcttcgc ccgggaagag
     9241 ttccacgatg cgctgcaaac gctcgaccgc cttggattgc aaagaatatt gaataaatat
     9301 ttcagcttga acgagcaagt cttcgagtgt ctgcctggat tgaacggctt ccagaatggc
     9361 gggcgaaggt tcgtgaggat gcttgctgtg cggttgcgga gcagccgtgt ccggattgtg
     9421 cgtggccgca ttggatagcc gcgattttac gcgcttaagg aaatcatcat cggcacgccc
     9481 actcagcaga tcgtagcgct cctggttgcg ggagtcataa ggatcgattt cgacaagctt
     9541 ttcgaaagct tcacagacct cgtggatttc gttattttcg agatgcaaat caaacaggtg
     9601 aacttcggcc tcaaaaaatt ttgtctcgcg attgagctcg ctatataaat ccgcccagaa
     9661 cttggccagg cgcgatgact ttggaaacgc ttccacaagt ttatcggcgg cagcggcaaa
     9721 ttcggattca tttcgcgcgg caagcatgct ttttttcgtg gcgacaagaa gttcgacggc
     9781 tttctcttcc tgaccggctc taaggaattc cgtggtcagc aagaagaatt tttccgctga
     9841 accgatcccc tgtctctgca tgctgtccag caggattcgc gcagtatcga gttgactgga
     9901 acgaatcagc gcgtcggcat aggtgtccag aaaaggcgcg tctctttcca cttcgctgag
     9961 cgggccaaga atcgcggcgg cggccgtcga atcgcccttg cgcattaagg cctgcgcata
    10021 atgcagcgcc gcgctgcgat ctccgggaat gatctcgtgc gcttttgcaa aaaattcaag
    10081 cgcctcgtca tcgttatcgg tggtcagctg cccggctctt aaatatccgc gcgctgcagc
    10141 cgtcagattg cccaggcgct cagccaagtt cgctacggta acgtgccgat cccgattttc
    10201 agaatcgagc tgcgcaatgc gcacgaagca ggccagcgcc tcttcccctt tgtcgctcag
    10261 ttcaaatgca agcgccgcgg acataaattg ttcgagagct tcttccgcgc gattctgttt
    10321 ttgcaaaagc aaggcgtatc gtgcaacgcg ttgcggaggt tgttgctgcg actttaaaaa
    10381 acgggtgtac agagcgagcg cttttggttc ttcccggggg gccaaaaaac ggtcaaacag
    10441 catcccgtaa taaacggctg cccggtccgg gcggttctgc atggtgtaca aatcgcccaa
    10501 cgattgtatg gattcaagat gatttggaaa atcgaccagc acagtctgat aggcctcaat
    10561 ggcctcgtca atgcttcgct tttcgaccaa gcgcttggct cgatctaatt gtttaacgat
    10621 atccacactc atgaaagctc gggcgggttc ctcgatggaa atacgttcgc gaattcgcgg
    10681 atcgacacac gaacccgcgt aaacactata tactgctgcg ccttagcgcg ccaaaccgat
    10741 cgcgcattgg ctgctcaata cggcaattcc cttgtcactc ctgataaact tccagcaatc
    10801 cgattggata atagttgctg aatagattta gaaaagggag aaggacttag ctgttctccc
    10861 tttttttatt ttattgcaat tcaaatgtaa aaaacgtgaa agtctccaat ccaattcact
    10921 cggttaagtt ccctgacata agatcaatat tgtctttccc cccaaccttc ctttgatcat
    10981 gtgagcctgg catgacctgc cgcgactcca tttcctgcat gcgtcgactt gtggcaaact
    11041 aaattgttac ggcgcgattc gaccaaccgt ttaggcagcg acctgttaaa cttgcgtccg
    11101 cagaacacat caaataggag aattttaccg gtgcttgacc gtgcgcgccg gcaggctaac
    11161 tgatggaccc acgtttcgtc cgcaattttt gcatcatcgc gcatattgat catggcaagt
    11221 ccactctggc cgatcgcctg ctggaactga ctggcgcgct tagccggcgg gagatgagcg
    11281 cgcaggtgct cgactcgatg gacctggagc gggagcgcgg catcacgatc aaggccaaga
    11341 gcattcgtct gcattattcg gcgaaggacg gaaacgaata ccagctcaac ttgatcgaca
    11401 cgcctgggca cgttgatttt tcatacgagg tttcgcgcgc gctctccgcg tgcgaaggcg
    11461 cgcttctggt ggtggacgcg agccagggcg tggaagcgca gaccgtcgcc aacacgtttc
    11521 tcgcggcgca ccacaatctc acgatcattc cggtcatcaa caaaatcgat ctccccgcgg
    11581 cgcagccgga tcaggccaag gaacaaattg aaaacgtgct ggccattccc gctgctgacg
    11641 cgctcttgat cagcgcgaaa agcggtattg gagtcgagtc cgtgctggaa gctattgtgg
    11701 cccgcatccc gccgccgaca ggaagtgcgg aagcgccgct gcaagcgctg atctttgact
    11761 cgtggttcga tccgtttcgc ggcgctgtgg ttctggtccg cgtgatgaac gggcggatga
    11821 cgaagcacat gaagattcgt ctctgtttca acgatcaggt gtatgaggtt gaagcgctgg
    11881 gcgccctgac gcccaagccc gtggtcctcg aagaactggt tgcaggggat gtcggcttcg
    11941 tgatcgccaa tatcaagcaa gtggccgatg cgcgcatggg cgacacggtt acggaggccg
    12001 cgcggcccgc gccgccgctc ccaggatttg aagccacaaa accgatggta ttcgccggct
    12061 tgtttcctgt gctagccacg gattacgaaa atttgcgcga tgctcttgga aaattgcaac
    12121 tgaatgacgc cgcgtttttc tacgagccag aaaattccgt ggctctcgga ttcggtttcc
    12181 gctgcggctt tttgggcctg ctgcacatgg aaattgcgca ggaacgcctc gagcgggaat
    12241 tcaacatcaa tctcatcact acagcgccca gcgtgcgcta ccgcatcacg accacagccg
    12301 gcgatatcgt ggaagtggac agccccacaa aatttccgcc gcccaatgaa atggccaaaa
    12361 ttgaagagcc ggtaatcacc gcgatgatca tcacagcgga agattcggtg ggaccgattc
    12421 tgcaactgtg cgaagagaaa cgcggcgtgc agaaaaattt tgagtatctc tctccgcagc
    12481 gcgtgatgct tacctatgaa ttgccgctga atgaaatcgt gctcgacttt tacgatcggc
    12541 tgaaaagcgc ttcgcgcggc tacgcttcgc tcgactatca cctcgcgggc tacaaggaag
    12601 ctgatctcgt gaagctcgac gtgctgatcg gcggcgaacc ggtcgacgcg ctggcgctgg
    12661 tctgccaccg cgaattctcg tatgaccgcg ggcgcgatct ggtttcgcgc ctgcgcaaac
    12721 tgattcctcg ccagatgttt gaggtgccca tccaggcagc catcggcagc cgcgtgatcg
    12781 cacgcgaaac cgtcgctgcg attcgcaaga acgtgattgc caaatgctac ggcggagaca
    12841 tttctcgaaa gcgcaaattg ctggagaaac aaaaagaagg aaaaaagcgc atgaagcgcg
    12901 tgggccgcgt ggacatcccg caggaagcgt ttctggccgt gctgaaagtt gcaagtacgt
    12961 cagacgagta gctttcaatt ctgctttgta atcttttttc gcgctatgcc tttattttac
    13021 gcagcccttt tccataaaaa agaggaagcg tcgcatttcg tggcataggc ttcagcctgt
    13081 ggggttagtc cttacaagga ccaaaatcca caggctgaag cctatgccac gaaatgcgat
    13141 tctgaaaaca agcggctgat cacttaatat ttattggggt tttggaatat cgcgggtgcg
    13201 cacttctgcg cctaatcggc cgaaggctaa ctgcacgttc acttccgcgc ccacggcgac
    13261 ctgttcagcg gagttcacta cattccctgc tgcatccgtg cagatcgagt aaccgcgctg
    13321 tagaacacgc agtgggccgc gctcatcgag ctgtacgcgc agccgctcca acatctggat
    13381 cttgccgcca agcacccgct ccaaacgcac gccaagttcc gccgagcgct gcataagcct
    13441 cagaccgagc gcgcgaatcc tcgcccgcaa atcaaaagaa gagatgcgtg tcgcggcaat
    13501 cgtatgccgg tggcgcaaca cctccaggcg gcctcgcaga gcgtctccga gacgcatagt
    13561 cagctcatca gtctgctgcc gatgttgacg cagcaaatct tccagacggc ggaagcccat
    13621 gtgggtagtc agctctttga ggtgatggcg gtgctcgagc aaaacatatc gcatgcgctg
    13681 cgaaattttg ctttccagtt cggagagatg ccgctgaaat tcctggcgag atttcacgac
    13741 gatttcggcg gctgcggaag gtgtggatgc gcgcacgtcc gccacaaagt ccgcaatcgt
    13801 cgtatcggtt tcgtgcccca cgccgctgat gattggaatc gagcaggaag ccatcgcgcg
    13861 agcaaccact tcctcgttaa atgcccagag atcttcgatc gatccgccgc ctcgcgcgag
    13921 gagaatcacg tctactgttt gtttgcggtt gaaatatttc agcgcgttgg cgatttcttc
    13981 cgccgctccg tcgccctgca cgcgcacggg atgcaggatc aagtgcaagt ttggaaagcg
    14041 ccgccgcaga atgcggataa tatcgcgcac ggctgcgccg gtcggtgagc tgatgacgcc
    14101 tacgcgcgca ggcagcaggg gaagcggctt tttgcgagat tggtcgaaaa ggccttcggc
    14161 ggccagccgc ttcttaagtt gctcaaacgc aagttgcaat gcgccaatcc ctgacggctc
    14221 gatgtgctcc acgtaaatct gatattcgcc acgcggctca tatacgctga tcgatccgcg
    14281 gacaattagt tttaacccat cctcaggcct gcatttcagc ctcatcgcct gcgtgcgaaa
    14341 gcacacgcac tttacttgcg ctttggcatc cttaagcgtg aaataaatat gtccggattg
    14401 cgcgggacgg cagttggaaa cttcgccctc cacccacaaa ttcgtgaatt gcgaggagag
    14461 cacaccgctg atgcgcgatg tcagttcgcc cacaccccag atcttccgct ccggtgccag
    14521 attcaattcg aattgtttta ccttttcagc cattgcacca gccacatgac caacgtaagc
    14581 accaagctaa gcaggataca cgtcaccacc ggaaaataaa acgatccatg tttcccctgg
    14641 tacgcaatgt cgcccgggag atgcccaagc cgtaacggga gccctccggg gcgcgcggcg
    14701 agccacacca gtgcgccaag cactgccaag accgccccag caaagaccat catccttccg
    14761 agttcgcgca taggttccgc cacattcaat tctacccaag atttggtgat tcaagaatta
    14821 gaggaagaaa gtgcgggcat ggaacatctg gtctccaaaa tgcacgtaaa agcctgatta
    14881 aacgcgttcg taacctttgt ttgctagttc ttggttgatc gatatgcctg gcaaagaaaa
    14941 accccgcctc tgaagacccg ggctacaaac tttgtagccc gggtcttcag gggcggggcg
    15001 tttctgtcgg tgaaaacaaa cttccaatca gcaacgcaaa cctaaatcaa ttcatggccg
    15061 gcaaaaaaat atcccaactc aaacgctgcc gtttctggag cgtcggagcc gtgcgtgcaa
    15121 ttgtttccga tgctcgtgcc gaggtcgcga cgaatcgtgc cgggggcggc cttggcgggg
    15181 tcggtggcgc ccatcgtgtc gcgccatttc aaaatcgcgc cttcggcttc gagcgccagc
    15241 acgacaacct tgccggaact cataaattcc gtcagttcgc cgaaaaaagg acgcgcggca
    15301 tgaaccgcat aaaatccctc ggcttccttt ttcgttagcc gcatcgactt gatcgcaacg
    15361 atttgaaatc cagccatatt aattcgtcca acaatttcag ctgccagccc gcgcccgaag
    15421 gcgtccggct taatgattgc tagtgttcgt tcgctcgcca ttcaatattt tctccatttt
    15481 cgaattttgt agcacaggct tcagcctgtg ggtttagctt gtgcaaaaat caaaagccgc
    15541 aggctgaagc ctgtgctact ggacacgctt ctttacttct taatcgcgcg cgccaaagtt
    15601 acaccgatat ctgccgggct ttgaatcgtc aagatacccg ctgcggccat ggctttgtat
    15661 ttttcagcgg ccgtgcctgc gccaccgctg atgattgcgc ccgcgtggcc catgcgccgg
    15721 cctggaggcg cagtctgccc ggcgattaat ccaacgacgg gcttttttac gttgtcttta
    15781 atgtaggctg cggctttttc ttccgcgtcg cctccaattt cgccaatcat cacgatggcc
    15841 tgtgtctcgg aatcttcatt aaagagtttg atcgcatcga tgtgcgtcgt gccgatgatc
    15901 ggatcgcccc cgatgccgat acaagtggat tgtcctatgc cgagttgcgt taactgatgc
    15961 acggcttcgt aggtcaaggt tccgctgcgg ctcacaacgc cgatgtttcc ctgcttgtga
    16021 atacgccccg gcatgattcc cattttgcat tttcccggcg aaataattcc ggggcagttc
    16081 ggtccgatca gtcgcgtctt ggaaagcttt agcgcggctg ccacgcgaac catatcgagc
    16141 gtaggaattc cttcggtgat gcacacgacg agcggaagtc cggcatcgat ggcctcaaga
    16201 accgcgtccg ctgcgtacgg cggcggaacg aaaatgatgg aagcatccgc acccgtttcg
    16261 cgtaccgcat ccgccacagt gttgaacatc ggcacgctct catgcttcgt gccacccttg
    16321 cccggggtaa cgcctgcaac cactttgctg ccgtactcaa tcatctgccc tgcatggaag
    16381 ctgccttcgc gccccgtcaa gccctgcaca accactcgtg tattttcgtt taccaaaacg
    16441 ctcatgataa tgatgttgag gaggtaaagg atgatgagga agtaagggaa taggccctcg
    16501 tcatcagaat tttctccatt ccttccatag attatgaagc ataaaaccca gcttgctata
    16561 ttccgagcct aacgttccgg ctcgctttgg ttccacaaaa ccttcatccg ttgccaaatc
    16621 gatccaaaac tttgtctcgg cacattcgcc aatcgcaatg atgagatgcc gtttaaattc
    16681 cgccgcagaa tttcgcttag cccaaccttc cacaatgttc gccgcaacag atcggctgca
    16741 attgcgaatt tgtcgcccaa gctcaaactg ttcctgcttc ggaaatgcct ttgtcacatt
    16801 cgaaacatcg agcgcaagcc tataagactg cttgtaaacc agcaggtcag aatatgtgcg
    16861 aacaactccc gctcgctcag gcatttcctt aacttccttt gattcctgta tttcctatat
    16921 ttccgttacc cttttcctgc cagcgcgaca attttatcgg ctccatcttt catgtctttc
    16981 gcgattgtga aattgcaacc tgagtctcgc aaaatttcct ggcctttttc cacgttggtt
    17041 ccttccattc ggatgaccac aggcagctta agattcaatt ccttcgctgc ggcgacgacg
    17101 ccgttcgcga gcacgtcgca ccgcaaaatt ccgccaaaaa tgttgatgaa aacgcccttc
    17161 acgcttttat cgctgattag aatcttgaaa gcatttttca cttgctcagc cgatgcgcct
    17221 ccgccaacgt ccaggaagtt cgccggcgag ccgcccgaca acttgataat gtccatcgtg
    17281 ctcatagcca ggcccgcgcc gttcaccatg cagccgacgt tgccatcgag cttgatgtaa
    17341 ttcagtttgt aggtggacgc ttcgacttcg agcggatctt cctcattcaa atcgcgcaag
    17401 tcggcgagat ccttgtggcg gtaaagtgcg ttgtcatcaa acgccatctt ggcgtctaga
    17461 gcaacgagct tgccgtctcc ggtcacgatc agcggattga tttccacgag tgacgcatcc
    17521 gtttcaagaa acgcgcgata gagcgcttgc aaaaatggtc cgacggcagc ggccgcatcg
    17581 ccggaaagac ccaatccata tgcgattttg cgcgtgtggt agggttgcag gccggtggcc
    17641 ggaagaatcg tttcgcgcag aatcagttcg ggattcgtgt gcgccacttc ttcgatttcc
    17701 attccgcccg cggtgctcgc cataatcacg ggcgctgcgc tggcgcggtc cacaagaatg
    17761 ctcagataaa gttcacgctt aatgtccaag ccttcttcga ccagcaagcg ctgcactacg
    17821 cgtccagcgg ggcctgtttg gggagtgacc agcgtcatgc ccatgatgga tttcgccagg
    17881 tcagcggctt ccgctgggga cttcgccaac ttcacgccac ccgctttacc gcggcctccg
    17941 gcatgaatct gcgcctttac cacaacaatt tttgtgccca accgctcggc cacggcgcgc
    18001 gcctgatccg gagtggtcac catttctccg cgaggcgtcg gaacgccaaa ctttctgaaa
    18061 atttcctttg cctgatattc gtggatcttc atcgtccgtc catcagtgct agaatttagt
    18121 tacattgaga gcctaagagt ttaacgggaa cgcccgaagc gggcaagacg aaaggaacag
    18181 tgcgcgttcg caacaaatgc taattctaaa tcatgcagcg agaaattatc tcgcaccagt
    18241 aggacaggct tcagcctgtc ttcttttgaa tttggactgt gatctgccaa tgaaagtcaa
    18301 aacaaaacag gctgaagcct gttctaccgg atcgaggctt gcccattcat gatgcgttcg
    18361 cttattcaaa aagtggaggc tggcgcggat ctttcccgcc aggaagccga aaacgcgatg
    18421 gaagaaattc tttccgggcg cgcgaatgag gagaccatcg ttgctctgct ttcttccctg
    18481 cgagcaaaag gcgaaacggt cggagaactt gtaggctttg ctcgcgccat gcggcggcag
    18541 gttgtgtctg tatttgccaa cggaatgcgg ccggatgaaa tgctggtcga cacttgcggt
    18601 acgggcggcg actcttctgg aacatttaac atttcgacgg cggcggcatt tgttgcggcc
    18661 ggagccagcg ttcacgtcgc gaagcatggc aatcgttcga tctcctcaaa atgcggttcg
    18721 gcggatgtac tggaagcgct gggtgtttcg ctggatattc caccggagcg cgtcggcgcc
    18781 gcgattcacg agatcggaat tggctttcta tatgcgcccg ccatgcacac ggccatgaag
    18841 cacgcgatgc cggcgcggcg caaactcggc cgcacagcat tcaacctgct cggacctctt
    18901 acgaatccgg cgggcgcacg cgcgcaagtt gtcggcgtgt tttcggacaa aatcgtggaa
    18961 aaagtggcgc tcacgctcag cgaactcgat gtcgaacgcg catttgtggt tcacggcgac
    19021 ggcgggttgg acgaaatttc tctggcgggt gacaccgcgg taggcgatgt tcaccgcgga
    19081 gtggtgagcc tttatcgaat tacgcccgaa cattttggcc tggaacgcgc ccccctggaa
    19141 tcaatttccg gaggggacgc ggcatataat gcggaaatca ttcgcgcgat tttttccggc
    19201 gaacgcggac cgcgccgcga tattgtcgtc gcgaacgcag ccgccgcgat tgtcgcgtca
    19261 ggccgcgcca cagatttcct ggaaggcgcg caacttgccg ccgcgtccat cgattccggt
    19321 gaagcccgca agaagcttga agcactgatc gcatttacgc atcgatagcg catagtggcg
    19381 cgggcttcag cccgggggtt ttgtccacgc aaggaccaaa atccgcgggc tgaagcccgc
    19441 gctactaaaa tctatagcga aagaaaattc ttcaggagtt gcattccgac ctcggtgagc
    19501 acggactcgg gatgaaattg cacgccctcc acgcacattt ttttatggcg caggcccata
    19561 ataattccat cggtttttgc ggagatttcc agatcggcgg gaaatgattt ttcttccacg
    19621 atcaatgagt ggtagcgcgt ggccacaaaa tcctgcggca aatcctcgaa aatcgttttg
    19681 ttatcgtgat ggatggggct ggtcttgccg tgcatgattt ccggagcgcg aatcacattc
    19741 ccgccgaatg cgtgcccaat tgcttggtgg ccgaggcaaa cgccaaggat gggaatctct
    19801 cccgcgaaac gtttaatcac gtcgatgcag atacccgctt cttgcggcgt gcctggaccg
    19861 ggagaaatca cgatccgttg cggcttcgtg gcggcaattt gatcgagcgt taatttgtcg
    19921 ttgcgatgta cttccagctt ttcgcccaac tggccgagga actgcgcgag gttataggta
    19981 aacgaatcgt aattgtccag aagcagaatc attgtgagtg gctcatttca gtcttcgcag
    20041 tccggccgag aatttcaaaa attcggattt aagtgatttt gtagcccgcg ttttcaaagg
    20101 cggggtttaa acgtagttaa agcagaactt cgtaccagca tctaaactct cgcctctgat
    20161 ccctcgtttg cactcgggac aagcgaggcg agctacagta gaacagcgag ccacaacaga
    20221 acttattatc gcgcagcgcg aatcgtatcc gggtcgggct cttcttcttc ctgagcgcac
    20281 ttgcggcgcg tgtcagaaac aaaatcatcc agcttgccct gatcgcgcat catcacaatt
    20341 ttatcgatgt agggccagcg cgcgtcgggc tcctgcttca gtatcgcttc atacgttttt
    20401 agcgatccaa gcaaacccgc ggtggcaatt gcctgctcat ccttaagctt gtcggggtgc
    20461 tcaatcatgt aagcggcgct ggaaatgacc atttgattga caatttcgcg cacgtgtcct
    20521 cgtaccggtt ttgaaagcgt accgaaataa tcatcgcaaa cggttaccgt gatatccgga
    20581 atatccacga tccattgaat gacccaatcg cgattatctt tggcatcttt cgcgaggggt
    20641 tcattttcga gttcatgcgc aactttcaca gcgcgagcgc gctcctcgac cgtcgacggc
    20701 ccgcgctggt gcggctggtc ctgttgctca ggctgctcct gctgttgagc gcgtgccgtt
    20761 cgcacacaga acaacccaag caacaaagtt gaaaacacta cggctctaat cgagtgcgcc
    20821 ctcatgattg tgaactcccg ctgacaatgg ccatgcgagt ggcccgcatc ccctaaaccc
    20881 taatagtaca cgaatttgac ggggagacga attcggtagg acaggcttca gcctgtcttc
    20941 ttttgacttc tgtaactttc acggaaattc aaaaccagac agtctgaagc ctgtcctacc
    21001 aaaccaaatg cccgtgtaca atggtcatgc ggaaaaacca cgacgagcac ttcaaaagga
    21061 acctatggca aagtttgaaa aacatatttt tatt
//
LOCUS       DASZMB010000112         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_5327533, whole genome shotgun sequence.
ACCESSION   DASZMB010000112 DASZMB010000000
VERSION     DASZMB010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5327533"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..703)
                     /gene="pssA"
                     /locus_tag="VG272_05155"
     CDS             complement(<1..703)
                     /gene="pssA"
                     /locus_tag="VG272_05155"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HWF13103.1"
                     /translation="MSDFEQHELGFPGARRASLRFRRGIHLLPAFMTVGNMLCGFYAV
                     MATVKGGSTDYDNAAKALGLAILFDSFDGFVARATGTSSEFGKQFDSLADMVSFGLAP
                     AVLAFAWGVRGIAETNFLDIRKLHEIGWWVVLTFVICCAWRLARFNIQGMAPGTGSKY
                     FVGLPCPAAAGLVAATVHAIKTPILDWRWSIFWMVVVLVDAALMSSTVRYRSFKDISM
                     LARQKSFLVIVMALLI"
     gene            complement(700..1311)
                     /locus_tag="VG272_05160"
     CDS             complement(700..1311)
                     /locus_tag="VG272_05160"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HWF13104.1"
                     /translation="MVKDGYWFAAPPLLLGIIAIVFHWNWLGGILLFLGLFVVFFFRD
                     PERTPPADPALIVSPADGRVVGIVEESFGGKTGHRISVFLSVFNVHVNRSPVGGRITA
                     IEYRTGKFYAAMRGRASAENEQNAFHVLTDRGEVVFTQIAGWVARRIVVWKAIGDSLV
                     RGERIGMIRFGSRMDIWLPDNVEILVKSGQSVAGGTSVLARWK"
     gene            complement(1733..2104)
                     /gene="rimI"
                     /locus_tag="VG272_05165"
     CDS             complement(1733..2104)
                     /gene="rimI"
                     /locus_tag="VG272_05165"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HWF13105.1"
                     /translation="MWSMESLEESASRWIAWVAERDGRVVGFLAGRPAADEFEILNLA
                     VGTEFRRKGIASQLIQTAIDTARSARVANIFLEVRASNEAGIALYRSLDFQPCGQRKN
                     YYRDPIEDAVLLIFNNFETVS"
     gene            complement(2218..>2549)
                     /locus_tag="VG272_05170"
     CDS             complement(2218..>2549)
                     /locus_tag="VG272_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13106.1"
                     /translation="VYKRLPGHGTGLQLVTDESILSLDEFLEMIKEKASDSVVLVSPT
                     PEVLPFSRIEEVLPGHRVITVSPALAPAIGKLGLERAKRGQLVDALTLDANYVRRSDA
                     EAAWKDR"
BASE COUNT          631 a          739 c          660 g          519 t
ORIGIN      
        1 agatcaacag agccataaca atcaccaaga acgatttttg gcgcgccagc atagaaatat
       61 ctttaaaact gcggtagcga accgtgctgg acattaaagc ggcatcgaca agtaccacca
      121 ccatccagaa aatcgaccat cgccaatcca gaatgggagt tttgatcgcg tgcacagtgg
      181 ccgcaactaa acccgcagcg gcggggcacg gcaggccgac aaaatacttg gagccggtgc
      241 ccggagccat tccctgaatg ttgaatcgcg cgaggcgcca cgcgcagcaa atcacgaacg
      301 taagaaccac ccaccagccg atttcgtgca gcttgcggat atccaggaaa ttcgtctcgg
      361 caatgccgcg cactccccag gcaaaagcca gaacagcagg cgcaagacca aagctgacca
      421 tgtccgccaa ggaatcgaac tgtttgccga attcgctgct ggtgcccgta gcgcgcgcca
      481 caaaaccgtc gaaggaatcg aacagtattg ccagacccag cgccttcgcg gcgttgtcgt
      541 aatccgttga accgcccttg accgtggcca tgactgcgta gaaaccgcaa agcatgttgc
      601 ccacggtcat aaaagcaggc aatagatgaa tgccgcgacg aaacctcaag ctcgcgcggc
      661 gcgcgccggg aaatcccaat tcgtgttgtt cgaagtcgct catttccagc gcgccaggac
      721 acttgtgccg ccggccacac tctggccgga cttcaccaga atttccacgt tgtcaggaag
      781 ccaaatgtcc atgcgcgaac cgaagcgtat cataccaatg cgttctcctc gcactaggga
      841 atctccaatc gccttccaga cgacaatgcg gcgagccacc cagccggcga tttgcgtaaa
      901 aaccacttct ccgcgatcgg tcaagacgtg gaaagcgttc tgttcatttt cggcggaggc
      961 gcggccgcgc atcgctgcgt agaatttccc tgtgcggtat tcgatggcgg taatgcgccc
     1021 gcccacggga gaacggttta cgtgcacatt aaaaacggaa agaaacacac tgatgcggtg
     1081 acccgtcttg ccgccgaacg attcttccac aatccccacc acgcgtccat cggcgggaga
     1141 aacgatcaac gctggatcgg ccgggggcgt gcgttccggg tcgcgaaaaa agaaaactac
     1201 aaagagaccg agaaaaagca ggatgccgcc gagccagttc cagtgaaata cgatggcgat
     1261 gattccaagc agcagcggcg gtgcggcaaa ccaatagccg tccttaacca tgcacgtcct
     1321 tgtcaggagg aaaaatttat ggcactcccg tagcgtccat aaatattcta tctcaattta
     1381 ggcaaggact ggagtgttag cgcggggccc gcggtttcat tcgttgcgca atatgccgtt
     1441 ccatctggtc tcttactttt agctttagct ttttgagggt ggcttcttgc tgaaggtctt
     1501 ccaggttcag gtaagctgag tgcgaaatct gtataagctg tgcttcgtat tctgagtgca
     1561 gttcgttcaa cttctggaat tcctggtcat atgtcagaag ttgctcgcgt atctcacggg
     1621 acaaggccgg cataatgtgc ctcctgttaa gcggaaaatg tgggcgggta ttacataaca
     1681 tggcgaaact agcacaatgc aaatctcccg ccaagtaaaa atccaccaaa aattaagata
     1741 ctgtttcaaa attgttaaaa atcaacagga ccgcgtcctc tatcgggtca cgatagtaat
     1801 tcttgcgctg cccgcaaggc tgaaaatcca ggctcctgta taacgcgatt ccagcctcgt
     1861 tggatgcgcg gacttccaga aaaatgttcg ccactcttgc ggagcgtgcg gtatcgatcg
     1921 cagtctgaat caactggctg gcaattccct tccgccgaaa ctctgtgcca acggcaagat
     1981 tcaggatttc aaattcatcc gccgcgggcc ttccagctaa aaatcccacg acgcgcccgt
     2041 cacgttcggc cacccacgcg atccatcgcg aagcagattc ttccaggctt tccatggacc
     2101 acattgacgc ttcgggactt tccttcagta tcgactgagc ccccgatgca tcagaaggca
     2161 gcatccgccg aatggtgaca gacctcggat gccaggctct gggcacgggc ccgggcatca
     2221 tcgatccttc cacgcggctt ccgcgtcgga gcggcgcacg taattcgcat caagcgtgag
     2281 cgcgtcgacc aattgtccgc gctttgcacg ttccaggccc agttttccga tcgcgggagc
     2341 cagcgcgggc gacaccgtaa ttacacgatg gccaggcagg acttcctcaa tccgagaaaa
     2401 gggcaacact tcaggtgtcg gcgacacgag gacgacagag tcgctggcct tctccttaat
     2461 catctccaga aactcgtcca gcgaaagtat agattcgtcg gtgaccaatt gcagaccggt
     2521 tccgtgccct ggtaagcgct tgtacacag
//
LOCUS       DASZMB010000113        17205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3665768, whole genome shotgun sequence.
ACCESSION   DASZMB010000113 DASZMB010000000
VERSION     DASZMB010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 17205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17205
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3665768"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(708..1976)
                     /gene="murA"
                     /locus_tag="VG272_05175"
     CDS             complement(708..1976)
                     /gene="murA"
                     /locus_tag="VG272_05175"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF006873.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HWF13107.1"
                     /translation="MDKLLVEGGRPLEGSVHISGAKNAALPAMAAALLTRDRVTLDNI
                     PRVRDIITMGKLLAHLSAIVETPEIPPTAMIIQADTVNGAEAPYELVKTMRASILTLG
                     PLIARSGHARVSLPGGCAIGARPVDLHLKALEQMGAEIGMSHGYVEARAPRGGRLPGG
                     HVFFDKITVTGTENILMAASLAEGETIIENAAQEPEITDLADLLIKMGADIEGGGTST
                     IRVRGKKELHGASHTIIPDRIEAGTFLVAGAITNGHLELTGCAPEHLTSIISKLRETG
                     VEIRETAPGTLEVRGAKRLVAADVRTEEYPGFATDMQAQFMALATQAEGTSAITETIF
                     ENRFLHASEMNRMGANISIEGRRAVVHGPTTLSGTNVIASDLRASASLVLAALVAGGE
                     TLIDRVYHIDRGYERIEEKLAAVGARIRRV"
     gene            complement(1981..2898)
                     /gene="murQ"
                     /locus_tag="VG272_05180"
     CDS             complement(1981..2898)
                     /gene="murQ"
                     /locus_tag="VG272_05180"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HWF13108.1"
                     /translation="MQTLKATEQRNPRTRGIDAKSTIEILRAIHREDTSVAKAVSAAM
                     PAITRAVDAMANAIQNGGRVFYIGAGTSGRLAVLDASELPPTYGTPASMVQAIIAGGR
                     RALTNAVEGAEDNRAQGARDLNKCKLNPKDAVVGIAASGGTPYVIGALEFANKKGAVT
                     IGITSNRGTPITKAARISIVTPTGPEVITGSTRMKAGTAQKLVLNMLSTATMIRLGHV
                     YDNWMIGVALTNQKLKARGLRILMEASGADVAESTRALRQSGHNLAAALIMLKTGASA
                     QEAKRLLRDTKGHVHSAINRTDQVARNSH"
     gene            complement(3168..6074)
                     /locus_tag="VG272_05185"
     CDS             complement(3168..6074)
                     /locus_tag="VG272_05185"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HWF13109.1"
                     /translation="MRKLSRWLLLLALFSMATTSHTQKSPRASDAPKPAGLSPEGERW
                     VSQTIKSMSLDEKIGQLFAVWAYGSFISTESQDYKDLLRDVEEKHIGSFAIQTQGSPV
                     GIVRSQVYPTAVLVNTLQRHAKVPLLVAADFERGTSMRVEEGASFPHAMAIAATGRPE
                     DAYTMGKITALEGKAVGVPWIFAPDADVNSNPANPIINTRSFGEDPARVSEFVAAFVR
                     GVEENGGLATAKHFPGHGDTSTDSHLDLPTVTSDRAHLERVELAPFRAAIAAGASTIM
                     TGHLAVPALEPDPDVPATMSPKITTDLLRTQMGFDGLVVTDALDMGGVTVRYPPGEVA
                     VRSILAGADVLLVPPVLDAALQAIHEAVASGRIPMSRLDEAVTRVLRAKAKLGLNKSK
                     LVDLEALAHDFNRPEFENAALDIADRGITLLRDDQHILPLDSTKPSRILLVAVSGDND
                     PYPAGNLEGEIRGRADSLTTIRMDTHFVRADTVKLPSPDSYDVAIAAIFVRVADRKGS
                     IGLPDDQAAVVDRLLAAGKPVIVACFGSPYLAQRFPLAKTWIAAFSTVDVAQWAMGRA
                     LFGQTAVSGRLPVNIPGVATLGAGLDLPAQPMKLRVANAQLAAKLQPAFDVLNRAVAD
                     HAFPGAVLAVGYNDELIVHAFGRQSYDVNSPTVAPDTIYDAASLTKPVVTSTLFAMQA
                     EAGRIGLDLPVARYIPEWNSGPNPDWRKTVTVRNLLTHTSGLAAHKDYFLTLHSKSEF
                     IAAICKEPLEYAPGSKTIYSDLGFILLGEILERATGKTIDQLAQEQIFAPLQMTNTEF
                     NPSKNLLARIAPTENDGTFRKRLLQGEVHDENAFAMGGVAGHAGMFTTASDLAAFCQM
                     MLNGGIYAHHRMLTRATISMFTAPQTIAGNARTLGWMSPTPNSSSGKYFSPRSFGHLG
                     YTGTSIWIDPDRKLFVILLTNRVNPTRENDKIAVVRPAVHDAVIESLGLMPAAK"
     gene            6271..7575
                     /locus_tag="VG272_05190"
     CDS             6271..7575
                     /locus_tag="VG272_05190"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HWF13110.1"
                     /translation="MKPLSYYYYFQTVRAVSSHLPMPLPILEKFFYKKSFLSLLILSV
                     FICVNPWFQSSELSAQTMTGINVLETQKFAALAGKHVGLITNQTGVDRDGRSTIDLLA
                     HARGVKLVALFSPEHGIRGALDARVSSSTDEATGLPIYSLYGETDRPTDAMLAGLDAL
                     VFDIQDAGVRFYTYITTMGYAMEAAAAHHISFYVLDRPDPLGGDRIEGPMLDPDRTNF
                     VGYFPMPVRMGMTLGEMAQMFNVEKKIDGDLHIIRMKNWRRNMWFHDAGLRWVNPSPN
                     LRSEESGVLYPGLEILQAGGVSVGRGTETPFEIVGAPWIRGEELARYLNNQKIPGVRF
                     EPRKFTPDSGLFKGGACEGVHVLLVDRNALQSMRMGIELAAALAKLYPGKFEATKIIT
                     LVGNVETIKQLQEGRDPAAIVNSWTFDLESFRKMRAKYLLYP"
     gene            7682..8608
                     /locus_tag="VG272_05195"
     CDS             7682..8608
                     /locus_tag="VG272_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HWF13111.1"
                     /translation="MKICSLLPSATEILFALGLGDHVAGVSHECDFPWEAQSRPVLIK
                     SRISHTESAAAIDRQVREFLERGESLYSVEMELLREIEPDIIVTQDLCHVCAATPDDL
                     GAALSYLPNKPKIVTLNPHSLADVCADIRAIGEATASIEQADELIAEFQKSIADVEQS
                     VAGLDRPRVLCLEWLDPPYVAGHWVPEMVERAGGIDVLGQRSKPSFRVDWEKVMESQP
                     EVIVIMPCGYNLAAADAEFRSFPLPTWWGEVPAVKEGRVFIVDASGYFSRPGPRLAVG
                     LRTLASAIHAEKRDSGAECTAEGPLVRVASVR"
     gene            complement(8775..10256)
                     /locus_tag="VG272_05200"
     CDS             complement(8775..10256)
                     /locus_tag="VG272_05200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HWF13112.1"
                     /translation="MQPYHRLALNMRLTSLDSAIIVLYLAGITVFGIAFRRGQSTVRD
                     YFLGDRTAPWWALAFSIVATETSTLTIIGTPALAFAGNLTFLQFVFGYLVGRVAIVLL
                     FLPHYFRGEFYTAYQLMERRFGAKVKAVAASTFLVTRVLAEGVRVAAIALVVTAALGT
                     GQRTSILLIMSLVLIYTFEGGMKAVIWTDVVQFLLYFSGSLAAFFLLWHKIPGGWQTI
                     VQSAEPANKFRIFDFSFSFTNPAKLYTFWSGVIGGTFFTMASHGTDQTIVQRLLAAKS
                     ERESKLALLASGVIVLAQFALFLVLGAMLYAWHGAPPLVAGQSYDSIFPDFIVTAMPS
                     GVRGLILAAILAVAMSNASGSLNSLAASSVVDFQHLRGSEKLVEDPARLLRRSRWLTV
                     VWGIVLAALGTIRWGPLLEAGLTIAAITLGILLGLFLLAFLFPRATATGVLAGMFVGF
                     LAILYIHFRTPLLWTWYVPFSAAITFLSGALVSLATKTEEQRR"
     gene            10321..11265
                     /locus_tag="VG272_05205"
     CDS             10321..11265
                     /locus_tag="VG272_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HWF13113.1"
                     /translation="MSYSLGFDGGGTKTHCVLIDSSGNVIGEGHGGPANPLRCGYDVA
                     FASLRDAAAAALASSNIQPTDISGVHAGLAGAGRRNVARRVFVFLAQEFPSALAQVST
                     DCEIALEAAAGSGPGVVLIAGTGSIAYGRNVAGETARAGGHGPWIGDEGSAFEIGRRS
                     VSAVARVRDLDAPASVLPEMISAALKCPDWDELLLRIMKNPDDVFPKLFPVVVAAANS
                     EDSAAREIMFASAIGLSNLAMVVIRRLGMKDDVFPLVKCGGVFGHCQMLDSLLDSVLA
                     SGALRAKISRLEISPALGAARMAARLTESSSQAAIHGA"
     gene            11255..12046
                     /locus_tag="VG272_05210"
     CDS             11255..12046
                     /locus_tag="VG272_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13114.1"
                     /translation="MAHEEHVDAQAAVSAAREAIPALMASSDQSVLRSLLDNPALDET
                     HVCLLLERKDLSGLLLEEISKRRTWRAAYRVRLGLAAHPHTPRLIAMRLLRELHLMDV
                     VRISLLTTSSMELRRLAEERVLTQLAQLPLGQKITLARRGSTRIAAGLVLHGPEQVAR
                     VALNNSFLTESQLLKTLAKETLPARIVAAVASHDKWSKVINVRVALLRHPQISPNHVP
                     ALLPGLPRRDIEDLLGLSRLQENVREILQEELTRRENSRGINLKI"
     gene            12154..12492
                     /locus_tag="VG272_05215"
     CDS             12154..12492
                     /locus_tag="VG272_05215"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWF13115.1"
                     /translation="MKIQSLDETITKPERRYWQRRKFRGNIEIDWGSTVLNASVRDIG
                     PQGLFVELIPPLWLGATFHARLMLNPVLLLDCIVARVDPARGIAVTFNVSADSGKAQL
                     ESLLLSLPAV"
     gene            12489..13076
                     /locus_tag="VG272_05220"
     CDS             12489..13076
                     /locus_tag="VG272_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13116.1"
                     /translation="MTRCLDCGSDRIADQCPACGLTSAAAELVVRRRLVKRTAIFLVG
                     IIVFVAASQVFPALELDSIQIFGGIVFFCALALGYWLDYRARKHQEIEVMKRIYFGMI
                     PVPWIFAGLLFLNGKLDTSRPIRVPATVIGKVATGAFPRSRRLIVTSWRYGRRFEHIA
                     VDQNDFDRFQRGDDIVIQAQKGAAGIPWIYGVFRQ"
     gene            13636..14940
                     /locus_tag="VG272_05225"
     CDS             13636..14940
                     /locus_tag="VG272_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWF13117.1"
                     /translation="MPQAKGEATVNSKQGRIEIDARMEDLSPATQFGPEYLTYVLWAV
                     TPEGRPKNLGEVLLNGTNSKLDVTTELQTFGLIVTAEPYFAVAQPSDVVVMENTIRND
                     TQGTIEPIDAKYELLQKGLYLQNINPSEVRPFVMDHKTPLEFYEAENAVRIAKWTGAE
                     EYAADTLHKAEEGVLNAQEELNGKIGKKTVSQNSRDAAQNAEDARLITIQKMQDEVAA
                     RDKAAADQRDAEARARTQQAEADRMNAERAKSQADQAAAEAQAEAQRQAQAREGAEQA
                     TQQAEQEKTQLREQLRQQLNMILETRESARGLIVNMSDVLFDSGQHSLKPGAREKLAK
                     VSGIILAHPGLSLQIEGHTDSVGGDEFNQQLSEKRADTVRDYLVAQGISGDVISAEGL
                     GKADPVATNGTAAGRQQNRRVELVVSGEPIGAVGPSASDNSR"
     gene            complement(14993..16387)
                     /gene="hydA"
                     /locus_tag="VG272_05230"
     CDS             complement(14993..16387)
                     /gene="hydA"
                     /locus_tag="VG272_05230"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HWF13118.1"
                     /translation="MGFDTIIRNGTVVTATDTYAADIGITGGQISAIAKTLPIENVTK
                     LIDAAGCIVIPGGIDVHTHLDMPFGGTTSADDFETGTRAAAYGGTTTLIDFAIQYKGQ
                     TLRHAFDTWMKKAEGKAVADYAFHCIITDLGDTQMEEMGALVREGVSSFKLFMAYPGV
                     FMMDDATIFKAMLQAAKHGGMICMHAENGGAIDVLVQRALAEGKTAPKYHALTRPTTA
                     EGEATSRAIALAEMAGAPVYIVHLSCDDALEKVREARDRGLPAYAETCPQYLHLSLDN
                     MDVPGFEGAKYVFTPPLREKWNQEKLWQGLARDTLQVVSTDHCPFCMKEQKELGKNDF
                     TKIPNGGPGVEHRLSLIYSGGVHGGKFSANRFVQLVSTAPAKLFGLYPRKGTIAVGSD
                     ADVVIFDPNEEQIISAKTHHMRVDYSMFEGMRVKGVTKTVLSRGRVVIENGNFTGRPG
                     AGEFLRRKTYTGAA"
     gene            16726..>17205
                     /locus_tag="VG272_05235"
     CDS             16726..>17205
                     /locus_tag="VG272_05235"
                     /inference="COORDINATES: protein motif:HMM:NF026042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13119.1"
                     /translation="MSLRETAPKLSPEKYEENFADIAPQMNTRQAVIEAARCLYCFDA
                     PCMQACPTRIDVPGFIKRIMTGNVRGAARVILEANILGESCGRACPTEVLCEGACVMI
                     EKGEKAIEIGRLQRFAVNHVHDRAIRLFQAGAPNGRRVACIGSGPASLACAATLARKG
                     "
BASE COUNT         3838 a         4396 c         5015 g         3956 t
ORIGIN      
        1 tgcgatcgcg tttggacaat ccggggcgct cccacacatc gccgaacaga accttctcgg
       61 tcaaatcgac cagctttggt gcaacatcgc ctaatctttc tcttgccgta ggcagcgcct
      121 tgtcgtttgt catagattct ccagggggtc cttaacagcg attgttttgc aaccgtcaaa
      181 atgtaccact actggactcg ggcagaatac tgaacatctg agcggcttgt tatagtatcg
      241 ccgtgtgcaa attttcatgc tcgcatggca agcatgacgc cgatcttgtg aaacacagtc
      301 aggaagacat cttttttgtg atcgaagcag gcccgtatat gcgaaccaaa aaactctgcc
      361 tcaaggctgt gatgacaagc agggaaggca agagcgtgtg gagtggcgag tatgcatgct
      421 cgatatgtgg attgcgcttt caacccgacc cgaaaggtcc tgccaagctc actcttgatt
      481 tttcaatcca tagtgagcag catcccgccg cagttgaatc tggagagaat cctgattgag
      541 tgggcagagt aaggcccaat tcgtaaattc aggtcgggtc gaacccgcca ccttgttgag
      601 ttttgggatt tggcggagtg gttggataaa gtcgaacttc aatacgcgcg cgtttgagaa
      661 tcgatgcgcg cggaaagcgc ggatctttag gaaacagtcg ttttccctta gacgcggcga
      721 atgcgggcgc ccacagctgc gagtttttcc tcgatgcgct cgtagccacg atcgatgtga
      781 tacacgcgat cgatcagcgt ttcgcctccg gcgactagcg cggcgagaac gagagacgcg
      841 ctggcgcgca ggtccgacgc gatgacgttg gtgccggaga gtgtggtagg gccgtgaacg
      901 acggcgcggc gaccttcgat ggaaatgttt gcgcccatgc gattcatttc gctggcatgc
      961 agaaagcgat tctcgaaaat ggtttcggtg atcgcggaag ttccttcggc ctgcgtagcg
     1021 agcgccatga actgggcctg catgtccgtg gcgaagccgg gatactcttc cgtgcgcaca
     1081 tcggcggcga ccagtctttt cgcgccgcgc acttccagcg tgccgggggc ggtctcgcga
     1141 atctcaactc cggtttcgcg cagcttggat atgattgaag tcaggtgttc cggcgcgcaa
     1201 cccgtaagtt cgagatgacc gttcgtgatc gctccggcga cgagaaatgt gccggcttcg
     1261 atacgatctg gaatgatggt gtgcgaagcg ccgtgcaatt ctttctttcc gcgcacgcga
     1321 atcgtggagg ttcccccgcc ttcgatatcc gcgcccattt tgatcagcaa atccgcgagg
     1381 tcggtaattt ccggttcctg cgcggcgttt tcaattatgg tttcaccctc cgcgagagat
     1441 gccgccatca aaatattttc cgttccggtg acagtgatct tgtcgaagaa aacgtggccg
     1501 cccggcaggc gtccaccgcg tggagcgcgc gcttccacat aaccatgcga cattccaatt
     1561 tccgcgccca tctgttccag cgcctttaaa tgcaggtcca cggggcgcgc gccaatggcg
     1621 cagccgccgg gcagggaaac gcgcgcgtgt ccgcttcgtg cgatgagggg gcccagtgtg
     1681 aggatcgaag cgcgcatcgt ctttacaagt tcatagggcg cttccgcgcc gtttacggta
     1741 tcggcctgga taatcattgc cgtgggcggg atttccggcg tctcgacaat ggcgctcaaa
     1801 tgagcgagaa gcttgcccat ggtgataatg tcgcgcacgc gcggaatgtt atcgagcgtg
     1861 acacggtcgc gcgtcagcag cgccgcggcc atggcgggca gcgcggcatt ttttgcgccg
     1921 ctgatgtgca ctgagccttc cagtggcctg ccgccctcaa ctaaaagttt atccattagc
     1981 ttagtgggag ttccttgcga cctgatcggt tcggttaatt gcgctgtgga catgtccctt
     2041 ggtatcgcgc aagagccgct tggcctcttg tgcgcttgct cctgtcttta acattatgag
     2101 ggctgcggcg agattgtgtc cggattgacg caaggcgcgt gtcgattcgg ctacgtccgc
     2161 cccggatgcc tccatcagaa ttcgcaggcc cctcgctttt aacttctgat tcgtcagcgc
     2221 cacgccaatc atccagttgt cgtaaacgtg gccgaggcga atcatcgtgg ctgtagagag
     2281 catgttgagg accaacttct gcgccgtgcc agccttcatg cgtgtagaac cggtgatgac
     2341 ttccgggccg gtcggggtga cgatgctgat ccttgcagcc tttgtgatcg gggtaccgcg
     2401 attggacgta ataccgatgg tgacggcgcc ttttttgttt gcaaattcga gcgcgccgat
     2461 tacgtaaggc gttccgccgc ttgctgcgat tccgacgact gcatcttttg ggttgagctt
     2521 acatttattt aaatcacgcg caccctgcgc gcgattgtct tccgctcctt ccaccgcatt
     2581 cgtgagtgcg cggcggcctc cagcaatgat ggcctgcacc atgctcgcgg gcgtgccata
     2641 ggtcggcggg agttccgagg catcaaggac tgcgaggcgg ccgctggtgc ccgcgccaat
     2701 gtagaaaaca cgtccaccgt tttgaatcgc gttcgccatg gcatcaaccg cgcgcgtgat
     2761 cgcgggcatc gcggcggaca cggctttcgc cacggatgta tcttcgcgat gaatcgcgcg
     2821 caaaatctcg atcgtggatt ttgcgtcgat tccgcgcgtg cgcggatttc gctgctcagt
     2881 tgcttttagc gtttgcattt tttgtggcgc cggcatcttg ccggcgggtt tgattgtcgg
     2941 cgcactttca gcaaaccgcc ggcgagacgc cggtgctgcg aaattctatc ttcgcacgct
     3001 ggcgagatga ttgagcgtgg cttatcgaag tgaatcgctc attcagttag ggggagtggc
     3061 ataggcttca gcctatggag tttggtactt gcttttgtta aacgccgcag gctgaagccc
     3121 gcgccacttt aggtgcttgc gcaagggaaa gtttagttgg atatgactta ctttgcggcg
     3181 ggcatgagtc ccagagattc gatcaccgca tcgtgtacgg cggggcgtac gactgcgatc
     3241 ttgtcatttt cgcgcgttgg attgacgcga ttcgtcagca gaattacgaa gagcttgcgg
     3301 tccgggtcaa tccagatcga cgtgccggtg tagcctaaat ggccgaagct gcgcggcgaa
     3361 aaatatttgc cgcttgaaga atttggtgtc ggcgacatcc atccaagggt gcgggcgttc
     3421 ccagcgattg tttgcggcgc ggtgaacatc gaaatagtgg cgcgggtcag catgcggtga
     3481 tgggcgtaaa tgccgccgtt cagcatcatt tggcaaaacg ctgcaaggtc tgacgccgtg
     3541 gtgaacatgc ctgcgtggcc cgctacgcca cccatcgcaa atgcgttttc gtcgtggact
     3601 tcaccttgca gaaggcgttt gcgaaatgtt ccgtcatttt ccgtgggagc gattcgcgcg
     3661 agaagatttt tcgacggatt gaattcggtg ttcgtcattt ggagcggtgc gaatatttgt
     3721 tcctgggcga gttgatcgat tgtttttccg gtggcacgtt ccaggatttc tccgagcaga
     3781 atgaaaccga gatcggaata aattgttttc gagccgggcg cgtattcgag aggctctttg
     3841 caaatcgcgg cgatgaattc actcttggaa tggagagtta gaaaataatc tttgtgcgcg
     3901 gccagcccgg aagtatgcgt cagcagattg cggactgtga cagttttacg ccaatcggga
     3961 ttggggccgc tgttccattc gggaatataa cgagcgaccg gtaggtcaag accgatgcgc
     4021 cccgcttctg cttgcatagc gaaaagcgtg gaagtaacaa ccggtttggt caacgatgcg
     4081 gcatcgtaga ttgtatccgg agcgactgtt ggagaattta catcatacga ttggcgaccg
     4141 aatgcgtgaa cgatcaattc atcgttgtaa ccgacggcca gcacggctcc agggaatgcg
     4201 tggtcggcga cggcacgatt cagcacgtca aacgcaggtt gcaattttgc tgctaactgt
     4261 gcattcgcca cacgcaattt catgggctgc gcggggagat cgagccccgc gccaagtgtc
     4321 gcaactccag gaatatttac tggcaggcgc ccactgacgg cggtttggcc gaagagcgcg
     4381 cgacccatcg cccactgtgc cacgtctacc gtgctaaagg cggcgatcca tgttttggcc
     4441 aaggggaatc gctgcgccag ataagggctg ccaaagcacg caacgatcac gggcttgcct
     4501 gctgcaagca agcgatcgac gacggcggcc tggtcatcag gcaggccgat gctgcctttg
     4561 cgatcggcaa ctcgaacgaa aatcgcggca atcgcaacgt catacgaatc gggggagggc
     4621 aatttcacgg tatcggcgcg cacaaagtgc gtgtccatgc gaatggttgt cagcgagtca
     4681 gcccgcccgc gaatttcgcc ttctagattt cctgcgggat acggatcgtt atcgcctgaa
     4741 acggctacga gcaggattcg tgaaggtttt gttgagtcga gcggaagaat gtgctgatcg
     4801 tcgcgcagga gcgtgatgcc gcggtcggcg atgtccagag cagcattctc aaattcggga
     4861 cgattaaaat cgtgcgcaag ggcttcgaga tcgacaagtt tcgatttgtt caatcccagc
     4921 ttggcttttg cgcgcagaac acgcgtcacc gcttcgtcca ggcgcgacat tggaatgcgt
     4981 ccggaggcga cagcttcgtg aatggcttgc agcgcggcgt ccagcacagg cggaacaagc
     5041 agcacatcgg cacccgcgag gatggagcga accgcgactt caccgggagg ataacgaacc
     5101 gtgacgccgc ccatatcgag cgcgtcggtt acgactaagc catcgaaacc catttgcgtg
     5161 cgcagcaagt ctgtcgtgat tttaggagac atcgtcgctg gcacgtcggg atcaggctcg
     5221 agagcgggca cagcgagatg gccagtcatg atcgtgcttg cgccagcagc gatggcggcg
     5281 cgaaacggtg cgagttccac gcgctcgaga tgcgcgcgat cgcttgtgac tgttgggaga
     5341 tcgagatgcg aatcggtgct ggtatcgccg tggcctggaa aatgttttgc cgtggcgagg
     5401 ccgccatttt cttccacgcc gcgcacaaat gcggcgacga actcggagac acgcgcggga
     5461 tcttcgccga acgagcgcgt gttaataatg ggatttgcgg gattgctgtt gacgtcggcg
     5521 tcgggtgcga aaatccaggg gacaccgaca gcttttcctt cgagggccgt aattttcccc
     5581 atcgtgtagg cgtcttccgg ccggccggtg gcggcgatgg ccatggcgtg cggaaatgaa
     5641 gcgccttctt cgacgcgcat cgacgtgccg cgctcgaaat cagcggcaac gagtagcgga
     5701 actttcgcgt gtcgctgcag cgtgttgact agaacagcgg tcgggtaaac ctggctacgc
     5761 acgattccaa ccggcgagcc ttgcgtttgg atcgcgaacg agccgatatg cttttcctcg
     5821 acatcgcgga gaagatcctt gtagtcttgg ctttcagtgg aaatgaaaga accgtaggcc
     5881 catacagcga agagttgccc gattttttca tcgagcgaca tagacttgat cgtttgagaa
     5941 acccagcgct cgccttccgg cgataggccg gcgggttttg gcgcgtcaga agcgcgtgga
     6001 gatttttgtg tgtggctcgt ggtagccatc gagaaaagcg cgagcagaag aagccagcga
     6061 cttaattttc gcatcgcgcg gattccattt tgttcgcccg catgcagcat tcatatatag
     6121 cacccgagcg ggagcgttac aaacaagatt ggaaccacgg atgcacacag ataaacatgg
     6181 ataaatgccg gattaaatta aaatttacgg taaacaaaca agattgtgaa taaaaatagt
     6241 acggctagcc atagagcctg aaaaacgaaa atgaaacctt tgagctacta ttattatttc
     6301 caaactgtgc gggccgtttc atcgcatttg ccaatgcctc tgcccattct agagaaattc
     6361 ttttacaaga agtcttttct atctcttttg attttatccg tgttcatctg tgtgaatccg
     6421 tggttccaaa gttccgagtt gtccgcgcag accatgaccg ggattaatgt tcttgagaca
     6481 cagaaatttg cggcgcttgc cgggaagcac gttgggctga tcaccaatca aactggagtt
     6541 gatcgcgacg ggcgcagcac gattgatttg ctggcgcatg cgcggggcgt aaagcttgtt
     6601 gcactgttca gtcctgagca cgggattcgc ggcgccttgg atgcgcgcgt ttcttcctcg
     6661 acggacgagg cgaccggatt gcccatttac agtctatatg gtgaaacgga tcggcctacg
     6721 gacgcgatgc tcgcaggcct cgacgcgctc gtgttcgata ttcaggacgc aggtgtgcgc
     6781 ttttatacgt acatcacgac catgggctac gcgatggagg ctgctgccgc gcatcacatt
     6841 tcattttatg ttctcgatcg acctgaccca ctgggtggag atcgtatcga aggtccaatg
     6901 ctcgatccgg accgaacgaa tttcgtcggc tattttccca tgcccgtgcg catgggcatg
     6961 acgctgggcg aaatggcgca gatgtttaac gtcgaaaaaa aaatcgatgg tgaccttcac
     7021 attattcgca tgaaaaattg gcggcgtaac atgtggtttc acgacgccgg gctgcgctgg
     7081 gtcaacccgt ctcctaacct gcgttccgaa gagtctggcg ttttgtatcc cggcttggaa
     7141 attttgcagg caggcggggt gtccgtaggg cggggtacag aaacgccatt tgaaattgtc
     7201 ggagctccat ggattcgcgg ggaggaactt gcgcgctatt taaataatca aaaaattcct
     7261 ggcgtgcgct ttgagccgcg aaaatttacg cctgattccg gcctgtttaa aggaggagcg
     7321 tgcgaaggcg tccatgttct tctggttgat cgcaatgcgt tgcaatccat gcgcatgggg
     7381 atcgaactcg ccgcggcact ggccaaactt tatccgggca aatttgaagc tacgaaaata
     7441 attacactgg ttggaaacgt ggaaacgatt aagcagcttc aagagggccg cgatccagcg
     7501 gcgatcgtga atagctggac cttcgatctg gaatcattcc gcaagatgcg cgcgaaatat
     7561 ttgctatatc cgtgattcgt ttgcgcccaa gagaccatgg tggctgtcca aacaaatctg
     7621 atccgcattc ctccgcagag aatcgaagaa actgcttcag ttccgtgtta aattgcggag
     7681 catgaagatt tgttcgttgc tgccatcggc cacggagatt ttatttgcgc tgggccttgg
     7741 cgatcacgtt gccggggtca gccacgaatg cgattttcca tgggaggctc agtccaggcc
     7801 tgtcctgatt aagtcgcgca tctcgcatac ggaaagcgcg gcagcgattg accggcaagt
     7861 acgggaattt ctcgaacgcg gcgaaagcct ttacagcgtt gaaatggaat tgctgcgcga
     7921 aatcgagccc gatattatcg tcacgcagga tctttgccac gtatgcgccg cgacgccgga
     7981 cgatttaggt gccgcgctca gttatttgcc aaacaaacct aaaattgtga cgcttaatcc
     8041 tcattctctc gcggatgttt gcgccgatat tcgggcgatc ggcgaagcga ccgcatccat
     8101 cgaacaagcg gacgagctta tcgctgaatt tcagaaatcg atagctgacg tggaacagtc
     8161 cgttgctggt ttggaccgtc cccgtgttct ctgcctcgaa tggcttgacc caccgtacgt
     8221 agcagggcac tgggttccgg agatggtcga gcgcgccggg ggaattgatg ttttgggaca
     8281 gcgcagcaaa ccatcctttc gcgtggactg ggaaaaggtg atggaatcgc agccagaggt
     8341 aattgtcatc atgccgtgcg ggtataatct tgcggcagcc gacgcggaat ttcgtagttt
     8401 cccactgcct acgtggtggg gggaggtgcc tgcagtgaaa gaaggccgcg tttttattgt
     8461 agacgcttcc ggatattttt cgcggccggg acctcggctg gcggttgggc ttaggacgct
     8521 tgcttccgcg atccacgcgg agaagagaga tagcggtgcg gaatgtacgg ccgaagggcc
     8581 gctggttcgc gtggcttcgg tcaggtaatg gtgtttggat ttgaacttct cttcaagtgt
     8641 acaaaacccc gaattgttcc tgaaaagtta tgtcattggg gcagaaaacc gagcctctga
     8701 agaggcgtgg ctgcagaatt gggtgccgac tcttgtagcc caggtcttca ggcccgggat
     8761 ttttgtttgg acggctaccg tcgctgttct tcggttttcg tcgccaggct tactagcgca
     8821 ccagatagga aagtgattgc ggcgctgaac ggaacgtacc acgtccacaa cagcggtgtg
     8881 cggaagtgga tgtagaggat cgcgaggaat cctacaaaca ttcccgctag aaccccagtc
     8941 gcggttgctc tcggaaaaag aaatgccaat agaaatagac caagcaagat gcccaaggtt
     9001 atggcagcta tggtcaatcc cgcttccaac aggggacccc agcgtattgt gccgagcgcc
     9061 gctaaaacga ttccccacac aacagttaac catcgagaac gtcgcagcag gcgcgctgga
     9121 tcctctacca atttctctga ccctcgcaaa tgttgaaagt ccacgacgct tgacgcggcg
     9181 agagagttca gcgagccgct ggcattggac atcgcgacgg caaggatcgc ggcgagaatt
     9241 aatccgcgga ctccggacgg catcgcggtg acaatgaaat cggggaagat agaatcgtaa
     9301 ctttgtcccg caacaagggg gggcgccccg tgccaggcat agagcatggc ccccagcacc
     9361 agaaaaagtg caaattgcgc aagtacaatg acgccgctag caagcagcgc gagcttgctt
     9421 tcgcgctcgg atttggcggc aagcaggcgc tgcacgatcg tttgatccgt accgtggctg
     9481 gccatggtaa aaaatgtgcc gccgatcacg ccggaccaaa atgtgtaaag cttggcggga
     9541 tttgtgaagc tgaaagagaa atcgaaaatg cggaatttat ttgcaggttc ggcagactgt
     9601 acaatcgttt gccagcctcc aggaattttg tgccacaaga gaaaaaacgc tgcgagcgaa
     9661 ccggaaaaat aaagcaggaa ctgtacaaca tccgtccaga tgacagcctt cattccacct
     9721 tcaaatgtat agatcaacac gagagacatg atcagcagga tggaagtgcg ctgtcccgtc
     9781 ccgagcgcgg cagtgacaac cagcgcgatg gccgcgacgc gcactccttc ggcaagaaca
     9841 cgggtcacaa gaaatgtaga cgccgcaacc gcctttacct ttgcgccaaa ccttctttcc
     9901 atcaactgat aggccgtata aaattctccg cggaagtagt gggggagaaa cagcaataca
     9961 atcgcgacgc ggccaacaag gtaaccgaaa acgaattgaa ggaacgtgag atttccggca
    10021 aaggccagcg ctggcgtgcc gataatggtg agcgtggagg tttccgtcgc gacaatcgag
    10081 aaggccagcg cccaccacgg cgccgttcgg tcgcccagaa aataatcgcg cactgtactt
    10141 tgtccgcgcc ggaaagcgat gccgaaaaca gtgattccgg cgaggtaaag gacgataatt
    10201 gcggagtcca gagaggtcag gcgcatgttg agggcgaggc gatgataggg ttgcatgcgt
    10261 tgtacgtcaa gcgcgcatct tatcttttct ttgcttgaat ttttacagta gtgaggtgga
    10321 atgagctact ccttgggttt tgatggtggc gggacgaaga cacattgcgt tttgatcgat
    10381 tcgagtggaa acgtcatagg cgaaggccac ggggggcccg cgaatccact gcgatgcggg
    10441 tacgatgtcg cgttcgcatc ccttcgagac gccgcggcgg ctgcgcttgc ttcctccaac
    10501 attcaaccaa cggatatttc cggagttcac gcggggctcg caggagcggg tcgccgcaat
    10561 gtggctcgac gcgtctttgt atttctggcg caggaatttc cgtccgcact ggcacaggtt
    10621 tcaactgatt gtgaaattgc gttggaggcg gctgcggggt caggaccggg cgttgtgttg
    10681 atcgcaggca ccggatcgat agcgtacggg cgaaacgttg cgggagagac agcgagggca
    10741 ggcggacacg ggccatggat tggcgatgaa ggaagcgcgt ttgaaatcgg aaggcgatcc
    10801 gtttctgccg tggctcgcgt tcgagatctg gacgccccgg ccagtgtttt gccggaaatg
    10861 atttctgccg cactcaagtg ccccgactgg gacgaattgc ttttgcgcat catgaaaaat
    10921 ccggatgatg tttttccaaa attatttcca gtcgtggtcg ctgcggcaaa ttccgaagat
    10981 agcgcggcaa gggaaatcat gttcgcgtcg gcgatcggcc tgagtaacct ggcaatggta
    11041 gtgattcgcc gactgggaat gaaggacgat gtatttccgc ttgtgaaatg cggtggagtg
    11101 tttgggcatt gccagatgct ggattcgctg ctcgattcag tgcttgcgag cggcgcgtta
    11161 cgcgcgaaaa tttcgcgtct tgaaatttcc cctgcgcttg gcgctgcgcg tatggctgcg
    11221 cggttaacag aatcctcatc gcaagccgcg attcatggcg catgaagaac atgtcgatgc
    11281 tcaggccgcg gtcagcgctg cccgcgaagc aattcccgct ctcatggcgt cgagcgacca
    11341 aagcgtcctg cgctctctgc ttgataatcc cgcgttagat gaaacgcacg tgtgtctcct
    11401 gcttgagcga aaggacctct ccggattgtt gctggaggaa ataagcaagc gccggacatg
    11461 gcgcgcggcg tatcgcgttc gactgggtct cgccgcgcat ccgcatacac cgcgcctgat
    11521 cgctatgcgc ctgctcaggg agttgcatct gatggacgtg gtgcgcatca gtttgctgac
    11581 aacttcttcc atggaacttc gccgtctggc ggaggaaaga gtactgacgc aattggcgca
    11641 attgcccctg gggcagaaaa tcacgctggc gaggcgcggt tcgacgcgta ttgccgcagg
    11701 ccttgtgctg catgggccag agcaggttgc acgcgtcgct ctcaacaatt cgttcctcac
    11761 tgaatcacaa ttgctgaaaa cactggcgaa agaaacgctt ccagcgcgaa tcgttgcagc
    11821 ggtggcaagc cacgacaagt ggtccaaggt aattaacgtt agagttgcat tgttgcggca
    11881 tcctcaaatc tccccgaacc atgttcctgc gctcttgcct ggattgccgc gccgcgacat
    11941 cgaagattta ctggggcttt cgcgacttca ggaaaatgtg cgggaaatct tgcaagaaga
    12001 acttacgcgc agggaaaaca gtaggggaat taatttaaaa atataagaag aataagatct
    12061 aatgtgcccg gactatgaat gctgggtggt ggaagcgtaa acttgacaca ccgtggctcg
    12121 gaacatagca tcccttaagg ggcgtgacgt gaaatgaaaa tccagtcctt ggatgaaacg
    12181 atcaccaaac cggaacgtcg ctattggcaa cgcaggaaat tccggggaaa tatcgagatc
    12241 gattggggat cgacagttct gaacgcgagc gtgcgggaca tcggtccgca aggtttattc
    12301 gttgagttaa tacctccctt atggcttgga gcgacgtttc acgcacgtct gatgctgaat
    12361 cctgttcttt tgttggactg catagttgcc agggtggacc ccgccagggg catcgctgtt
    12421 accttcaacg tttccgcaga tagcggtaaa gcgcagctgg aatcgctgtt gctgagtttg
    12481 cccgccgtat gacgagatgc ctcgattgcg gttcggatcg cattgccgat cagtgtccgg
    12541 cgtgcggcct gacttccgct gccgcggagc tggtagtgcg ccgccgtctg gtgaaacgta
    12601 cggctatttt tctggttggc attattgttt ttgtcgccgc aagccaggtt tttcccgctc
    12661 tcgaacttga ttccattcaa attttcggcg gtatcgtatt tttctgcgct ctcgcgctcg
    12721 ggtattggct ggactaccga gcgcgcaagc atcaggaaat tgaagtcatg aaaaggattt
    12781 atttcggaat gattcctgta ccttggattt ttgccggcct cctgttttta aatggaaagc
    12841 tcgacacctc gcgcccgata cgtgtgcctg cgaccgtcat cggcaaggtt gcaaccggag
    12901 ccttcccgcg tagcaggcgc ctgatcgtta cttcatggag atatggacgc cgcttcgagc
    12961 acatcgcggt ggatcagaac gatttcgacc gctttcagag gggagacgac attgtgattc
    13021 aagcgcagaa aggcgctgcg ggaatcccct ggatttatgg cgtttttcgt caatagagaa
    13081 ccgcgaacgg aatcgtaccg cctcatccac aattcggaat ggcaataaat gaaaaaacgc
    13141 gtatcgtgcc gtttggaatt atttaattcc tcgcttcatt ttatgaaata catgaaacca
    13201 tttagtttca aacgtgtcgg tatctcgctt gggagtggta tttacgactc caatgtttcc
    13261 tgcattaata cagagccaac tatcttgata taggctgaat cccgtatcga tgattttcct
    13321 gccctgatgt ttgatctttc atatacaaat tctctcccag cgcctcatgc agatttatat
    13381 aattttgtga tagatggagc taggccaatg aaacatacta aatgtattag aacagcgaag
    13441 acattagcca caaccgtcct tctatgttca ctgagcatgc tcgctcaaga atccgggacg
    13501 aatcagcatg ttcaagtaga tcattctggg aacgggccag tattccgcgt aacggttgtg
    13561 gagcgaacca ccaaagcgat taattatcgt caccgtagcg gttctacccc ggtcgatttt
    13621 cgaggaactg aattgatgcc gcaggctaaa ggcgaagcga ctgtcaatag caagcaagga
    13681 cggattgaga tcgatgcaag aatggaagat ctttcgccgg caacacaatt cggccctgaa
    13741 tatctaacgt atgtattgtg ggctgttacg ccggaaggcc gcccaaaaaa tctgggtgaa
    13801 gtattgctta atggcacgaa cagcaaactt gatgttacga cggaactcca aacgtttgga
    13861 cttatcgtga cggctgaacc ctattttgct gtcgcccaac cgagcgacgt tgtcgtgatg
    13921 gaaaacacca tccggaatga cacgcaggga acgattgagc caattgatgc aaagtatgaa
    13981 cttctgcaga agggcctata tcttcaaaat attaatcctt cagaagttcg cccgttcgtg
    14041 atggatcaca agactccttt ggaattctac gaggctgaaa atgctgtgcg catcgcgaaa
    14101 tggacgggcg cggaagaata tgccgcggat accctgcaca aggcggaaga gggtgttcta
    14161 aatgcgcaag aagaactcaa tggtaaaatc gggaaaaaga cggtttccca aaattcgcga
    14221 gatgccgcgc aaaatgcaga agacgccagg ctaatcacga ttcaaaaaat gcaagatgaa
    14281 gtggcggccc gagataaagc agccgcggat caacgggacg cggaagccag ggcgcgtacc
    14341 cagcaagccg aagcggatcg gatgaacgcg gaacgagcta aatcacaagc ggatcaagct
    14401 gcggcagagg cccaggcgga agcgcagcgg caggcgcaag ctcgtgaagg agcagagcag
    14461 gcgacgcagc aggccgaaca ggaaaagacg caacttagag aacaacttcg gcaacagttg
    14521 aacatgattt tagagacgcg agaaagcgct cgtgggctga tcgtcaacat gtcagatgta
    14581 ttgttcgatt ccggtcaaca ttctttgaaa cctggagcac gagaaaagct ggccaaagtg
    14641 agcggcatta ttctggcgca tcctggtttg agtcttcaga ttgaaggcca cacagatagc
    14701 gtgggtggcg atgaattcaa tcagcaacta tccgagaaac gcgcagatac cgtacgcgac
    14761 tatctggttg cgcagggaat ttctggagat gtaatttcag ccgaaggatt gggcaaagcc
    14821 gatccagtgg caacgaacgg cacggctgcc gggcggcaac aaaaccgtcg cgttgagttg
    14881 gttgtctcgg gcgagccaat cggggcggtt ggaccttcgg cttccgacaa ttcgcgctag
    14941 tcagaaacaa attggtcgcg gaactgtggt taagaaaaac gtgcaccgtg ctttatgcgg
    15001 cgccggtgta cgtttttctg cgcagaaatt ctccggcgcc gggccggccg gtgaagtttc
    15061 cgttttcgat aacgacccga ccacgggaca gcacggtttt tgtcacgcct tttactcgca
    15121 taccctcgaa catcgaataa tccacgcgca tgtggtgtgt tttggcgctg atgatttgtt
    15181 cttcgttggg atcaaaaatc acgacgtccg cgtccgaacc gacggcgatc gtgcctttgc
    15241 ggggatacag gccaaaaagt tttgccggag cagtagacac aagttgcacg aagcggttcg
    15301 ctgaaaattt tccgccatgc acgccaccgg aataaatcaa gctgaggcga tgttcgacac
    15361 cgggcccgcc attgggaatt ttcgtgaaat catttttgcc cagttctttt tgttccttca
    15421 tacagaaagg gcaatgatcg gtggagacga cctgcagggt gtcgcgtgca aggccctgcc
    15481 aaagtttttc ctggttccat ttttcgcgca gtgggggagt gaacacatac ttcgcgcctt
    15541 caaagcccgg cacgtccata ttgtccaacg ataaatgcaa atattggggg cacgtttccg
    15601 catacgcggg caggccccgg tcgcgcgctt cacgcacttt ttcgagagcg tcatcgcagg
    15661 acaaatgcac gatgtacaca ggcgctccgg ccatttcagc gagagctatg gcccgcgaag
    15721 tcgcttcacc ttcggcggtc gtcggccgag tgagcgcgtg atacttgggg gcggtttttc
    15781 cttcggccaa ggcgcgttgc accagcacgt caatcgcgcc accattttcc gcgtgcatgc
    15841 agatcatgcc gccgtgcttc gcagcttgca gcatggcttt gaaaattgtg gcgtcatcca
    15901 tcatgaagac gccggggtag gccataaaaa gtttaaaaga tgaaacgcct tcgcggacca
    15961 acgcgcccat ctcttccatc tgcgtgtcgc ccaggtccgt gataatgcaa tggaatgcgt
    16021 aatcggccac ggcttttcct tcggcttttt tcatccaggt gtcgaaggcg tgccgcaacg
    16081 tttgcccctt atattgaatt gcaaaatcga tcagcgttgt ggttccgcca tatgcggcgg
    16141 cgcgcgtgcc ggtctcgaaa tcatcggcgc tggttgttcc gccgaatggc atatccagat
    16201 gcgtgtgcac atcgatcccg ccgggaatta cgatgcagcc agcagcgtca attaattttg
    16261 tgacgttttc aatgggaaga gttttggcga tggctgaaat ctgtccgccg gtgatgccga
    16321 tgtcggctgc gtacgtatcg gttgcagtta cgaccgtgcc gttgcggata atcgtgtcga
    16381 accccatcgc ttcccccaat gccgctggat tgaagacttc ggcatcttag gctatgcggt
    16441 gaaagcgtgg caagttgtgg agtggcgtta cgtggcacag gcttcagtct gtgggtttta
    16501 attcttgcaa acactaaatc cacaggctga agcctatgcc acgtaaaccc gcattacatg
    16561 ggcggcggca atgcggttta tgccgcaggc cggaaattcg ttcgcaattg ctgttatcgt
    16621 gcggtgctag aatgacggag taattctggc cctgatgccc ggtggaataa ttacgtcccc
    16681 tgggacgttt atttcagggg aagacatcga acttgaggtg tgcgcatgtc ccttcgcgaa
    16741 accgctccga aattatctcc tgaaaaatat gaagaaaatt ttgcggacat cgcgccgcag
    16801 atgaacacgc ggcaagccgt cattgaagcc gcgcggtgcc tctattgttt tgacgctccc
    16861 tgcatgcagg cctgccccac gcggatcgat gttcccggtt tcatcaaacg cattatgacg
    16921 gggaatgtgc gcggcgccgc gcgcgtgatc ctcgaagcaa atattcttgg cgaaagttgc
    16981 ggacgagcct gtcccacaga agttctttgt gaaggtgcat gcgtgatgat cgagaagggc
    17041 gaaaaagcga ttgagattgg ccgtctccaa cgttttgcag tgaaccacgt gcatgaccgc
    17101 gccattcgac tattccaggc gggcgcgccg aatggacgcc gcgtggcttg tatcggatcc
    17161 ggtcccgcct ctttggcatg cgccgcgact ctagcgcgaa agggc
//
LOCUS       DASZMB010000114        11150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_399047, whole genome shotgun sequence.
ACCESSION   DASZMB010000114 DASZMB010000000
VERSION     DASZMB010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399047"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1896
                     /locus_tag="VG272_05240"
     CDS             <1..1896
                     /locus_tag="VG272_05240"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWF13120.1"
                     /translation="IVAGDQQPATVHALAHAMNQALGNIDKTVHFTAPLEVVADIQWD
                     AMGQLAADIRAGNVTTLLILGSNPVYDAPADLDFKSIIPKVAFSVRLGLYEDETSARC
                     HWHIPAAHELESWGDARGYDGTVSVIQPLIAPLYGGKSELDFLALLNGQSSKVSHDLV
                     HDYWQAQRTSVPRFDAFWEKTLRDGVVADSALPVKQVSVKVGIGSEAPTAPAQGLEIV
                     FRPDHTIWDGRFANNGWLQELPKPLTLLTWDSVAMVSPKTAERLGVKTETAVELKYQG
                     RTIVAPVWVMPGHADESVTVFLGYGRTKAGRVGTATGFDAGWIRPLATPWIGTGLDVR
                     KTGDTWALASTQTQNTMEGREPIHIATLDEYQKNPKFAQADVQENPLSLYPEVKYEGN
                     AWGMAIDLNACTGCGGCVVACQSENNISVVGKKEVRIGRHMHWIRIDRYYEGDMDDPG
                     TYHEPVLCMHCEDAPCEVVCPVAATVHSSEGLNEMIYNRCVGTRYCSNNCPYKVRRFN
                     FYLYSDWDTPSMFGMRNPNVSVRSRGVMEKCSYCVQRINAVKIEAEKQDRPIKDGEIV
                     TACQQSCPSEAIVFGNINDPNSAVSKLKAKNRNYKLLEDLNTRPRTSYLAKLRNPNPE
                     IEKAAAV"
     gene            1908..3299
                     /gene="nrfD"
                     /locus_tag="VG272_05245"
     CDS             1908..3299
                     /gene="nrfD"
                     /locus_tag="VG272_05245"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HWF13121.1"
                     /translation="MADSPTNKLAAEEILNAPILGPGHDFASVTDKISAIVLTKRSPH
                     WWIAGFLISFALVSLLMVSVTVILVWGVGVWGITIPIGWGFAIINFVWWIGIGHAGTL
                     ISAILLLLRQQWRTSINRFAEAMTLFAVACAGLFPLFHMGRPWLGYWLFPYPDTMKLW
                     PNFRSPLVWDAFAVSTYATVSLLFWFIGLIPDLATLRDRAKNRPAKIIYGLLAMGWRG
                     SAVHWERHETASLLLAGLATPLVLSVHTIVSFDFAFGIVPGWHSTIFPPYFVAGAIYA
                     GFAMVLTLAIPIRKLFHLEDFITLRHLDNMGKVLLATGLIVGYGYMMETFTAFYSGDV
                     YERFMTLNRMRGPYAVIYWMLIACNIVTPQLLWFRKVRAKIGALFAIAMVVNLGMWLE
                     RFVIVVTSLHRDFLPSSWGMYYPTRWDFATFFGTIGLFLFLFFLFIRYLPLISIFEMR
                     TLVASEEEKKVAS"
     gene            3343..3882
                     /locus_tag="VG272_05250"
     CDS             3343..3882
                     /locus_tag="VG272_05250"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HWF13122.1"
                     /translation="MKQRETIYGLMAEFATPEQLLEAAHRTHDAGYRRIDAFAPFPIE
                     GLAHAVGFKGTRLPLVVLLAGILGGFSGFFLQYYAAVISYPINVGGRPLNSWPAFIPV
                     TFELTILFASVAAVFGMLAMNGLPTPYHPVFNVPRFALASRDRFFLCIKARDPMFDLE
                     ITKNFMQTLHPREVTEIEP"
     gene            3872..4483
                     /locus_tag="VG272_05255"
     CDS             3872..4483
                     /locus_tag="VG272_05255"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWF13123.1"
                     /translation="MSLSANSRKPFAGRWILAAASLLFVCAGCRLDMHVQPRYNPYDP
                     TDFFGDGQSARLPVEGTVPRGDLKLGPNELFYSGKLNGQSSEAFPYPVTQQIVERGRE
                     RYNVFCSPCHGFTGDGDGMIVQRGFRHPPSFHDDRLRSAPVGHFFDVITNGFGVMYPY
                     GYRVPVADRWAIIAYVRALQLSRQVPIADVPDAERAKLTSESK"
     gene            4480..5664
                     /locus_tag="VG272_05260"
     CDS             4480..5664
                     /locus_tag="VG272_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13124.1"
                     /translation="MIDTRESTLKLEGLPMRVGSAGAVVIAICVAAGVADKTEFFHAY
                     LVAFLFCLGITLGSMALLMVQHLTGGRWALVIRRILEASSRTLPFMAIAALPVIVGMK
                     TLYTWARPGQTDPAVIAKLGYLNPQFFVIRMIFYFAVWFTLTYFLNKWSRQEDTGGAD
                     LALWMRLEGLSGIGLVIYGFTVTFAGIDWAMSLEAHWYSTIYGLLFMVGQALTAMAFA
                     IIMLIWLSQHRPLSQVVRPAQFQDLGSFLLTFIMLWAYMEFSQFLIIWGGNLTDEIPW
                     YIRRMEGVWGHVAMLLIILCFAFPFFLLLFRHVKTRTRSLFIVSFIVLLMRLMDMYWM
                     ILPAFGGGSIRLTWMDVALPVGMSGIWFAYFIWQLQRMPLLPAHDPRMEEIIEQAAEQ
                     HG"
     gene            5657..6094
                     /locus_tag="VG272_05265"
     CDS             5657..6094
                     /locus_tag="VG272_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13125.1"
                     /translation="MDKQHNASATPGYETRDANTGAILNFLVILAVVLVASGLICWGM
                     FRIFSTHAVDRPATDSPFADTRQLPLGPQLQVNPREDWLKFRQGQEDSLHTYGWENRT
                     AGIVRVPIEEAMELLVKKGLPVQNAPQTQPAEEKKPAPEGSKK"
     gene            6094..6933
                     /locus_tag="VG272_05270"
     CDS             6094..6933
                     /locus_tag="VG272_05270"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HWF13126.1"
                     /translation="MRFRIHAALLLALSGLAAFPAAAQQSTTPAILKKVGIQQNLNSQ
                     IPPDLEFRDETGKTVRIGDYFGKKPIVLSLVYFDCPALCTEVLNGQLRTMNAISLNLG
                     SDFEAVTVSFEPKDTPALAKAKRDVYIGQYGRPGAAEHWHFLTGGQQSIDALTNITGF
                     QYAYDPAIKQYAHAAAIMVLTPEGRIDRYFYGVIYPARDFRLGLVEASEGKIGTITDH
                     ALLYCYQYDPMTGKYGVVIINVLRISGGLTVLALGIFMAIMFLRERKRSTGIPPAVDS
                     RAR"
     gene            6961..7920
                     /gene="coxB"
                     /locus_tag="VG272_05275"
     CDS             6961..7920
                     /gene="coxB"
                     /locus_tag="VG272_05275"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HWF13127.1"
                     /translation="MQSLPFQPEQASSIAKSVDQLYMFLTVVTLFFTTLIFSLIFYFM
                     IRYRRRSPDERPKPIEGNVPLEVLWTAVPTLIVALIFVWSSMLYFKNSEAPKGSMEIF
                     ITGKQWMWKVEHPEGQREINEVHVPLGRPVKLTMTSEDVIHDFFVPAFRVKKDVLPGR
                     YTSLWFTATKVGTFHLFCAQYCGAFHAGMMGSIIVQKPDEYERWLAGGVPGESMEQAG
                     EKVFQSSGCSTCHVADGSGLGPSLLGVYGRPVKLTTGETVTADDAYIHESILLPKAKI
                     VMGYTPIMPTFQGQLTEEQLNDVIAYIRALGKSQPKSKEEGKQ"
     gene            7917..9545
                     /gene="ctaD"
                     /locus_tag="VG272_05280"
     CDS             7917..9545
                     /gene="ctaD"
                     /locus_tag="VG272_05280"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HWF13128.1"
                     /translation="MSTATLEPRVHTPRNYLNADYGWKSWLFTLDHKRIALLYLISVT
                     FFFLIGGSFAVMMRIHLTEPQGALVQPETYNKLFTLHGVIMIFFFLIPSIPAVLGNFL
                     VPLMVGARDLAFPKLNLLSWYIYIVGGMFTLYSAIAGGVDTGWTFYTPYSSTYSNTQV
                     TAVAIGVFVTGFSSILTGLNFIVTIHKMRAPGLTWFRMPLFLWSMYATSLIMVLGTPV
                     LAITIFLLALEHLFHVGIFSPALGGDPLLFQHLFWFYSHPAVYIMILPAMGVVSEIIA
                     CFSRKRIFGYSIVAFSSVAIAVLGFLVWGHHMFVTGQSVYAGLIFSLLSFLVAIPSAI
                     KVFNWTATLYKGSISYEAPMLYALGFIGLFTMGGLTGLFLASLATDVHLNGTYFIVAH
                     FHYIMVGGAVMGYLGGIHFWWPKISGRKYPNMWAGIAAVIIFIGFNLTFFPQFILGYL
                     GMPRRYAFYPAEFQVLNVMSSAGATVLGVGYLIPLIYLIWSMRYGPIAEPNPWGATGL
                     EWKTPSPPPTENFAEIPVVTEEAYAYGPEEVSGD"
     gene            9538..10221
                     /locus_tag="VG272_05285"
     CDS             9538..10221
                     /locus_tag="VG272_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HWF13129.1"
                     /translation="MTDAESHAQQGMAHQFEGLEQQHEADMLGIWVFLITEIMFFGGM
                     FASYAIYRWLYFAAFDGGSHILDIHLGAANTIVLLGSSLTMALSVRSAQTGNRRATIM
                     FLISTMILGGIFLGVKAYEYDQKFVEHVVPALDWGPEGETLARLAPGGVQHAEIYFIF
                     YFAMTGLHAIHMIIGMGLLLWLVFKARENTFTPHYFAPVEVVGLYWHFVDIVWIFLFP
                     LLYLIGGRY"
     gene            10221..10550
                     /locus_tag="VG272_05290"
     CDS             10221..10550
                     /locus_tag="VG272_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HWF13130.1"
                     /translation="MSHPIVPQKTYILVFLCLIGLTLLTTGVAFIDLGPFNTVAALAI
                     AFSKMALVMLFFMGVRHSSGLIRIIVITGFFWLALLMALTLADYHTRSWNPVPQAWSS
                     AAPPTHP"
     gene            complement(10584..10784)
                     /locus_tag="VG272_05295"
     CDS             complement(10584..10784)
                     /locus_tag="VG272_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_267462186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HWF13131.1"
                     /translation="MDLAMATIICPTCGYASTETVPTDSCLFFYECRGCSAVLKPKSG
                     DCCVFCSYGDRGCMFRKPEKSK"
BASE COUNT         2302 a         3034 c         3029 g         2785 t
ORIGIN      
        1 attgtggcgg gagaccagca gccagccacc gtgcatgcgc tcgctcatgc catgaaccag
       61 gcgttgggca atatcgacaa gactgttcat ttcaccgcac cccttgaagt tgtcgcggac
      121 attcaatggg atgccatggg ccagctggct gccgatattc gcgccggcaa cgttaccact
      181 ttattgattt tgggcagcaa tccggtgtat gacgcgccag ccgacctgga tttcaaatcc
      241 atcatcccga aggtggcgtt cagtgtccgc ctcggcctct acgaggacga gacatcggcg
      301 cgttgccact ggcatattcc ggcggcacac gaactggaat cctggggcga tgcgcgcgga
      361 tatgacggca cggtttccgt aattcagccg ctgatcgctc cgctctatgg cggaaaatca
      421 gagctcgatt tcctggcgct cctgaacggc cagtcttcta aagtgagcca cgacctcgtt
      481 catgattatt ggcaggcgca acgcacgagc gtgccgcgct tcgacgcatt ttgggaaaag
      541 acgctgcgcg atggcgtggt cgcggactcg gctctccccg tgaagcaagt ctcggtgaaa
      601 gtaggaattg gctcggaagc gccgactgct ccggcgcaag gtctcgaaat tgttttccgg
      661 ccggatcaca ccatctggga cggacgcttt gcgaataacg gctggctcca ggaattaccc
      721 aagcctctca cgttgctgac atgggattcg gtggcgatgg tcagtccgaa gacggctgaa
      781 cgtcttggag taaagaccga aaccgccgtc gaactcaaat atcaggggcg cacgattgtc
      841 gcgcccgtat gggtgatgcc gggacacgcg gatgaaagcg ttaccgtctt cctcggctac
      901 ggccgaacga aagccggacg cgtagggacc gccacagggt ttgacgcagg ttggattcgc
      961 ccccttgcaa cgccatggat tggcaccggg ctcgatgttc ggaagacagg cgacacgtgg
     1021 gcgctggcct ccacgcaaac acaaaacacg atggaaggcc gcgaacctat tcacatcgcc
     1081 acgctggatg agtaccaaaa aaatccgaaa tttgctcagg ccgatgtgca ggaaaatccg
     1141 ctgtcgcttt atcccgaagt gaaatatgaa ggtaacgcat ggggcatggc catcgatttg
     1201 aacgcctgca caggttgcgg cggctgcgtc gtcgcttgcc agtcggaaaa taatatttca
     1261 gttgttggca agaaagaagt ccgaatcggg cgccacatgc actggatacg catcgaccgc
     1321 tactacgaag gcgatatgga cgatccgggc acgtaccacg agccggtcct ttgcatgcac
     1381 tgtgaagatg cgccatgcga agtggtttgc cccgtggccg ccactgtcca tagcagcgag
     1441 ggcctgaacg aaatgatcta caaccgctgc gtcggcacgc gctattgctc aaataattgc
     1501 ccatacaaag tgcgacgttt caacttttac ctttactcgg attgggatac gcccagcatg
     1561 ttcggaatgc gcaatcccaa cgtcagtgtg cgtagtcgcg gcgtgatgga aaagtgctcc
     1621 tactgcgtgc agcgcatcaa cgctgtaaag attgaagccg aaaagcagga tcgtcccatt
     1681 aaggacggcg agatcgttac tgcctgccag caatcctgcc cgtcggaagc catcgttttc
     1741 gggaacatca acgatccgaa cagcgccgtt tccaaattga aagccaagaa ccggaattac
     1801 aaattgctcg aagatttgaa tacgcggccg aggacctctt atctggcaaa gctgcgcaat
     1861 ccaaatccag agattgagaa ggcagcggca gtctagcgga atgacacatg gcagatagcc
     1921 caacaaacaa actggccgcg gaagagatcc tgaacgcgcc gattctcggc ccgggacacg
     1981 attttgcttc cgttacggac aaaatcagcg cgattgttct gaccaagcgc agcccacatt
     2041 ggtggattgc cggattcctg atttcatttg ccttggtctc ccttctgatg gtgtcggtca
     2101 ccgtcattct cgtctggggt gtgggcgtct ggggcatcac gattccgatt ggctggggtt
     2161 tcgcgattat taacttcgtc tggtggatcg gaatcggcca cgccggcacg ctgatttccg
     2221 cgattttgct cctcttgcgc cagcaatggc gtacgtccat taaccgtttc gccgaagcca
     2281 tgacgctatt cgcggtggcc tgcgcgggat tatttccact gttccacatg ggtcgtccgt
     2341 ggctcggtta ttggctgttc ccgtacccgg acacgatgaa gctgtggccc aacttccgaa
     2401 gtccgctggt ctgggacgcg ttcgcggttt cgacctacgc aacagtgtcg ctcctgttct
     2461 ggttcatcgg cttgattcct gatcttgcca cgctgcgcga ccgcgcaaaa aatcgcccgg
     2521 caaaaataat ttatggcttg ttggcgatgg gatggcgcgg ttcggcagtt cactgggaac
     2581 gacacgaaac ggcttcgctg cttctggcgg gtcttgcgac tcccctggtt ctatccgtac
     2641 acacaatcgt aagctttgac ttcgcgtttg gcatcgtgcc cggctggcac tcgactattt
     2701 tcccgccgta ctttgtcgcc ggcgcgatct acgccggttt tgccatggtg ctcacgcttg
     2761 ctattccgat acgaaaattg tttcaccttg aagacttcat caccctgcgc catctggaca
     2821 acatgggcaa ggtgctgctt gctaccggcc tgattgtcgg ctacggctac atgatggaaa
     2881 cgtttaccgc gttctacagc ggcgacgttt atgaacgctt catgaccttg aaccgcatgc
     2941 gcggaccgta tgcggtgatt tactggatgc taatcgcctg caacatcgtg acgccgcaac
     3001 tcctatggtt ccgaaaagtc cgcgcgaaaa tcggcgcgct gttcgccatt gccatggtcg
     3061 taaatctggg catgtggctg gaacggtttg tcattgtcgt caccagcttg catcgtgatt
     3121 tcctgccgtc atcttggggc atgtactacc cgacacggtg ggactttgcc acgtttttcg
     3181 gaacgatcgg acttttcctg ttcctgtttt tccttttcat ccgttacttg ccgctgattt
     3241 ctatttttga aatgcggaca cttgtggcga gcgaggaaga aaagaaagtg gccagctgat
     3301 gacgtccgct gaattttact ttttgcccat ggttaaggtg cgatgaagca acgcgaaacc
     3361 atttacggat tgatggcgga atttgccacg cctgagcaat tgctcgaagc cgctcaccgg
     3421 acgcatgacg ccggctaccg gcgcatcgat gccttcgccc catttccgat cgaaggcctc
     3481 gcgcatgccg tcggattcaa gggcacgcgg ctgccgcttg ttgttctgtt ggcaggaatc
     3541 ctgggcggat tctccggatt cttcctgcaa tattacgccg ctgtgatcag ctaccctatc
     3601 aacgtgggcg gcaggccact gaacagctgg cccgcgttca ttcccgtcac ctttgaactg
     3661 actattcttt ttgcctctgt ggctgctgta tttggcatgc tggcgatgaa tggactgccg
     3721 acaccttatc atccggtgtt caacgttccg cggttcgcgc tggcttcgcg cgatcgtttt
     3781 tttctgtgca tcaaggcgcg cgatcccatg ttcgatttgg aaatcaccaa aaattttatg
     3841 cagacgctcc acccgcgcga ggtaaccgaa attgagcctt agcgcaaatt ccagaaaacc
     3901 gtttgcgggc cgctggatac tggccgccgc gagccttctt tttgtgtgcg ccggctgccg
     3961 tctcgacatg catgtccagc cgcgttacaa cccgtacgat ccgactgatt tttttggaga
     4021 cggacaatcc gctcgcttgc cggtggaggg cacagtaccc cgcggcgacc tgaaactcgg
     4081 gcccaacgag ctcttttatt ccgggaaatt gaacggccag tcttcggaag cgtttccgta
     4141 tcctgtcact cagcaaatcg tggaacgcgg gcgcgaacgc tacaacgtgt tctgttcgcc
     4201 gtgccacgga tttacgggcg atggagacgg aatgatcgtg cagcgcggct tccgccatcc
     4261 gccatcgttc catgatgacc gtctgcgctc cgcccccgtt ggacattttt ttgacgtaat
     4321 cacgaacggt tttggggtga tgtatcccta cgggtatcgc gttccggtcg cggatcgctg
     4381 ggccatcatc gcctatgtgc gcgcgctgca actcagcagg caggttccta tcgcggacgt
     4441 gccggacgca gagcgggcca aattgacgag tgaatcgaaa tgatcgatac gagagaatcc
     4501 acgttgaaac ttgaaggtct gccgatgcgc gtgggctccg cgggcgctgt cgtcattgcg
     4561 atttgcgtcg ctgccggagt tgcggataaa acggaatttt tccacgcgta tctcgtcgca
     4621 ttcctcttct gcctcggcat cacgcttggc tcgatggccc tcctgatggt gcagcacttg
     4681 actggaggaa gatgggcgct cgtcattcgc cgcattctcg aagccagcag ccgcacgctg
     4741 ccgttcatgg ccattgccgc gcttccggtg atcgtgggca tgaagacgtt gtacacttgg
     4801 gcgcgccctg ggcaaaccga tccagccgtc atcgcgaaac tcggctatct caatcctcaa
     4861 tttttcgtga ttcgcatgat tttttatttt gccgtctggt tcacgctgac ctatttcctg
     4921 aacaaatggt ccaggcaaga agacactggc ggcgcggatc tcgcgctctg gatgaggctg
     4981 gaaggattga gcggaatcgg tttggttatc tatggattta ccgtaacatt tgcgggaatc
     5041 gattgggcga tgtcgctgga ggcgcactgg tattccacaa tttatggttt gctgtttatg
     5101 gtcgggcagg cgctcaccgc gatggccttc gctatcatca tgttgatctg gctctcgcag
     5161 cacaggccgc tttctcaagt cgtaaggccg gcgcagtttc aggacctcgg cagcttcctg
     5221 ctgacattta tcatgctctg ggcctacatg gaattttcgc aattcctgat catctggggc
     5281 ggcaacctca ccgatgaaat tccctggtac attcgcagga tggaaggcgt ttggggccac
     5341 gtcgcgatgc tcctcatcat tctgtgtttt gcgtttccat ttttcctgct gctgttccgt
     5401 catgtgaaaa caaggacacg ctccttgttc atcgtgtctt tcattgtcct cttaatgcgc
     5461 ctcatggata tgtactggat gattttgccg gcgtttggcg gaggcagcat ccgcctcacg
     5521 tggatggacg tggcgctccc ggtcggcatg tcagggatct ggttcgcgta ttttatctgg
     5581 caactgcagc gcatgccgct tttgcccgcg cacgatccgc gcatggaaga aattatcgag
     5641 caggcggctg aacaacatgg ataaacaaca caacgcttcc gcgacgccgg gttatgaaac
     5701 tcgtgacgcg aacacgggcg caattcttaa ttttctcgtg attctcgctg tggtgcttgt
     5761 agcctcagga ctgatttgct ggggcatgtt tagaatcttt tccacgcacg cggtcgaccg
     5821 gccggcaacc gattcacctt ttgccgatac ccgtcagctt cccctcgggc cgcagttgca
     5881 agtgaatccg cgcgaggatt ggctgaaatt ccgccaaggc caggaagatt ctctgcacac
     5941 ctacgggtgg gaaaaccgca ccgcgggaat tgtgcgcgtg ccgatcgaag aagccatgga
     6001 attgctcgtg aaaaagggat tgcccgtgca gaacgcgccg caaacccagc ccgccgagga
     6061 aaagaaaccg gcgccggaag ggagtaagaa ataatgagat ttagaatcca tgccgcgctg
     6121 cttttggcgt tgtccgggct tgccgctttt ccagcggctg cgcaacagag caccacgccc
     6181 gcgatcttga aaaaagtggg catccagcaa aatctgaatt cacagattcc gccggacctc
     6241 gaatttcgcg acgagactgg caagacggtg cgaatcggcg attattttgg aaaaaagccg
     6301 atcgtgcttt cgctcgttta tttcgactgc cccgcgctgt gcaccgaagt attgaacggg
     6361 cagctgcgca cgatgaatgc aatctcgctc aatctgggct cggactttga agccgtcacc
     6421 gtaagttttg agccgaaaga tactcccgct ctcgccaaag cgaagcgcga cgtttacatc
     6481 ggccagtatg gccgccccgg cgccgcggag cactggcatt ttctgaccgg cgggcagcaa
     6541 tcgattgacg cgctcacgaa cattaccggt tttcaatacg cctacgatcc cgcgattaaa
     6601 cagtacgcgc atgccgccgc catcatggtg ctgactcccg agggccgcat cgaccgctat
     6661 ttttacgggg tgatttatcc ggcgcgcgat tttcgcctgg gcctcgtgga agcgtccgaa
     6721 ggcaaaattg gaaccatcac cgaccacgct ttgctctatt gctaccagta cgatcccatg
     6781 actggaaaat acggcgtggt catcatcaat gtgcttcgta tttccggcgg attaacagtg
     6841 ctggcgttgg gaatttttat ggcaattatg tttcttcgcg aacgcaaacg ttcaaccgga
     6901 attccgccgg cagtcgattc aagggcgcgg tgaagataac gacgtgagga tacgatcttg
     6961 atgcagagtc tcccatttca gccggaacaa gcttcttcca ttgcgaagtc ggttgaccag
     7021 ctgtacatgt ttttgacggt cgtgacgtta ttttttacga ccctgatttt ctcgctcatt
     7081 ttttatttca tgatccgcta tcgccggcgc tcgccggacg aacgtcccaa gccgattgag
     7141 ggcaatgtcc ctctggaagt cctctggaca gccgtcccga cgctgattgt tgcgttgatt
     7201 tttgtctgga gctcgatgct ttatttcaag aattcagaag ctcccaaggg ctcgatggaa
     7261 attttcatca ccggcaagca gtggatgtgg aaggtggagc atcccgaggg gcagcgcgaa
     7321 attaacgaag ttcacgtgcc tcttggacgt cccgtgaagt tgacgatgac ttcggaagac
     7381 gtaatccacg atttcttcgt tcccgcattc cgcgtgaaga aagatgttct gcctgggcgc
     7441 tacacatcgc tctggttcac tgcaaccaag gtgggaacat ttcatttatt ttgtgcgcag
     7501 tattgcggcg cgttccatgc cggaatgatg ggctcgatca ttgtgcagaa accggacgaa
     7561 tatgagcgct ggcttgcggg cggagtgccg ggcgagtcga tggaacaggc cggcgaaaaa
     7621 gtatttcagt ccagcggctg ctccacttgc catgtggcgg acggttctgg ccttggtccg
     7681 tcgctcttgg gtgtttacgg acgcccggtc aaattgacta cgggcgaaac ggttacggcg
     7741 gacgacgcat acattcacga atcgatcctt ctgcccaagg ccaaaatcgt aatgggctac
     7801 actccgatca tgccgacgtt tcagggccaa ctgacggaag agcaactcaa cgatgtgatt
     7861 gcctatatcc gtgcgctcgg aaagtcgcag ccgaaatcaa aggaagaggg gaagcaatga
     7921 gtacggccac gctggaacca cgcgtgcaca ctccgcgcaa ttacctgaac gcggactacg
     7981 gctggaaatc ctggctattt acgctggacc ataagcgcat cgcgctgttg tatctgatat
     8041 cggtcacgtt ctttttcctg atcggcggtt cgtttgccgt gatgatgcgc atccatttga
     8101 ccgaaccgca gggcgcgctc gtgcaaccgg agacctataa caaattattc acgctgcacg
     8161 gcgtgatcat gattttcttt tttctgattc catcgattcc cgcggttctc ggaaatttcc
     8221 tggtgcccct gatggtaggc gcgcgcgatc tcgcctttcc gaagctcaac ttgctgagct
     8281 ggtacattta catcgtcggt ggcatgttta cgctgtactc ggcaattgcc ggcggcgtgg
     8341 acaccgggtg gacattttat acgccctaca gtagcacgta ctcgaatacg caagtcacgg
     8401 ccgtggcgat cggcgtgttc gtcaccggat tttcctcgat tctcacgggc ctgaatttca
     8461 tcgttacgat tcacaagatg cgcgctccgg gtctgacctg gttccgcatg ccgctcttcc
     8521 tttggtccat gtatgcgacc agcttgatta tggtactcgg tacgccagtg ctggccatca
     8581 cgattttcct tctcgcgctt gagcacctct ttcacgtcgg cattttcagc ccggcgcttg
     8641 gcggcgatcc acttttattt caacatttgt tctggttcta ctcgcacccg gctgtgtaca
     8701 tcatgatttt gccggccatg ggtgttgtca gcgagatcat cgcgtgcttc tcgcgcaaga
     8761 gaattttcgg ttactcgatt gtggcgtttt ccagcgtggc gatcgcggtc cttggatttc
     8821 tggtctgggg acatcacatg ttcgtcaccg gacaatcggt gtatgccggg ctgattttct
     8881 ccttgctcag tttcctcgtt gccattccat ccgccattaa ggtgttcaac tggaccgcga
     8941 ctctttacaa gggatcgatt tcctatgaag cgccgatgct ctacgccttg ggcttcattg
     9001 gcctcttcac aatgggcggt cttacgggat tgttcctcgc ttctctcgcg acggacgtgc
     9061 acttgaacgg aacctatttc atcgttgctc acttccatta catcatggtc ggcggcgcag
     9121 tgatgggtta cctcggcggg atccatttct ggtggcccaa aatttccggc aggaaatatc
     9181 cgaatatgtg ggcaggcatc gccgcggtta ttatttttat cggcttcaac ctgactttct
     9241 ttccacaatt tattctcggt tacctgggca tgccgcggcg gtacgcgttt tatcccgcgg
     9301 agtttcaagt gcttaacgtg atgtcttcag cgggcgcgac ggttttgggc gtcggctatc
     9361 taattccttt gatttacctg atctggtcga tgcgttacgg tccgattgcg gagccaaatc
     9421 cgtggggcgc cacagggctc gaatggaaga caccgtctcc tccgcccacg gaaaacttcg
     9481 cggaaattcc cgtggtcact gaggaggcat acgcatacgg tcctgaggag gtctccggtg
     9541 actgacgccg aatcgcacgc acaacaggga atggcgcatc agttcgaggg cctcgaacag
     9601 cagcatgaag ctgacatgct cggaatctgg gttttcctga ttacggaaat catgtttttt
     9661 ggcggcatgt ttgcctccta cgcgatttac cggtggctat atttcgccgc ttttgatggc
     9721 ggcagccaca ttttggacat tcaccttggg gcggccaata caatcgtcct gctcggaagc
     9781 agtttgacga tggcgctttc cgtgcgttcc gcgcaaacag ggaatcgccg cgccaccatc
     9841 atgttcctca tctcgacgat gatcctcggc ggaatattcc tcggcgtaaa agcctacgaa
     9901 tatgaccaga aatttgtcga gcacgtcgtt ccggccctcg actggggacc cgaaggtgaa
     9961 accttggcac gtctcgctcc aggcggcgtt cagcacgcgg aaatctattt cattttctat
    10021 ttcgcaatga cgggactgca cgcgattcac atgatcattg gcatgggcct gttgctctgg
    10081 ctggttttta aagcgcggga aaacaccttt acgccgcatt attttgcgcc ggtcgaggtt
    10141 gtgggcctct attggcactt cgtcgatatt gtgtggattt tcctgtttcc attgctttat
    10201 ttaataggcg ggcgctacta atgtcacatc cgattgtgcc gcaaaaaacc tatatccttg
    10261 ttttcctgtg cctgattggg cttacgctac tgactacggg tgttgccttc atcgatctcg
    10321 ggccttttaa taccgttgca gctctggcca tcgcgttttc gaaaatggcg cttgttatgc
    10381 tcttcttcat gggggtaagg cacagcagcg gcctcatccg gatcattgta attaccggat
    10441 tcttctggct ggcgttgtta atggccttga ctctggccga ttatcacacc cgctcctgga
    10501 atccggttcc gcaagcgtgg tcttcagctg cgcccccaac ccacccataa attccacaaa
    10561 ataaattaaa aattggaagt gtctcatttt gatttttccg gcttgcgaaa catgcatcca
    10621 cggtctccgt atgagcaaaa tacgcagcaa tctccggatt tcggcttgag cacggcacta
    10681 catccccgac actcgtaaaa gaacaagcaa gagtctgtcg gcaccgtctc tgttgaggca
    10741 tacccgcagg ttggacagat aatggttgcc atggctaagt ccatgctgag tctataatcc
    10801 cgcgattttc gttcagggtc aaaatcaaaa ggagtcacta ccaaaaattg gatttagaaa
    10861 cagaaaaccc gtccgttttc acggacgggt ttccaagagg ggagaatagt tccgtgccta
    10921 tgcgcggaag gcactagaac tctatgtcct cgcggaaagt ctagagtagg acaaaccgga
    10981 atgtcaagtg gagaaaagat tgaatctaaa tgactacaag ttatttataa tcaatagatt
    11041 acgtctggca gttaaagtaa gggatgaact tgtcgagcga ggttttcaac aggcgcttta
    11101 gataactagt agaagtcctt tgccttgatt ggcttgctgg aaattcccaa
//
LOCUS       DASZMB010000115         5960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4506054, whole genome shotgun sequence.
ACCESSION   DASZMB010000115 DASZMB010000000
VERSION     DASZMB010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4506054"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..822
                     /locus_tag="VG272_05300"
     CDS             94..822
                     /locus_tag="VG272_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWF13132.1"
                     /translation="MIRTAIVAVFLSLYTLVLGPPLILYTLLTRSANLMYWVGVKGVV
                     FITRVAGLRVQVEGIEHIPRGVCMFAANHTSNADAAAIVGAIPRRLAIFGRKSLFDIP
                     IVGLAFRLAKFVPVDRGNRDAALAAVKQGVEYIKSGSSFLVYPEGTRSPNGRLLRFKK
                     GTFVMAIEAGVPIVPVACSGAHRVMEKNSLVIHPGKVTVRFLEPIDASKYSVEQRDDL
                     AKRVHDLIAASLPEDQKPALQAVL"
     gene            complement(948..1991)
                     /gene="lpxD"
                     /locus_tag="VG272_05305"
     CDS             complement(948..1991)
                     /gene="lpxD"
                     /locus_tag="VG272_05305"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HWF13133.1"
                     /translation="MKLGELAARLGCTLEGDPQTEIHGVAGIADARPGEVTFLSNPKY
                     SRELATTLASAAFVGNEVGVKRNPGLPSLFVLRSGNPYFDFARAVELFHAPQTYLPGI
                     HATAVVAKSATIGEGAHIGPHCFVDEGVEIGRNAVLHSLVTIYRNAKIGDDFLAHSQV
                     VVRESCSIGNRVILQNGVVIGGDGFGFAKRADGRWHKIVQQAPVIIEDDVEIQANSCI
                     DRATVGETRIRRGAKIDDLVLVGHACEVGEDSILCGQVGLAGSTRVGNRCILAGQVGS
                     AGHLEIGDGATITSKTGVHSEVPPGAIHSGYPNLENKFWLKLMAALKRLPEMQKTLRH
                     VAEELDRIKTARK"
     gene            complement(2194..2676)
                     /locus_tag="VG272_05310"
     CDS             complement(2194..2676)
                     /locus_tag="VG272_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13134.1"
                     /translation="MRLRAPASTLKKVVVLLLDRTTYRGYLNPGNLGGADALDVLTPE
                     GAHRTLAVKDVRSVYFVRDFTDDFEPERKAFLSRPKLDGLWVRLKFRDNDSIEGVVPN
                     DLLSLLDRGVQITPPDLHGTTLKIFIPRTALSEMTVLGVVGVARRKSAAPPQPTLFSE
                     "
     gene            2887..4506
                     /gene="murJ"
                     /locus_tag="VG272_05315"
     CDS             2887..4506
                     /gene="murJ"
                     /locus_tag="VG272_05315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HWF13135.1"
                     /translation="MTHILTHKKQILKSASIISIITVVSRILGYVRDQRLTLLLGTTG
                     IADSFVLAYRIPNILRRLVGEGSMTASFIPVFTDYMRNRSKEETWAFANRLFWTFSVV
                     LAALTVLGVIFSPLIVRMFSLFGKNQVQVEAIYLNRLMFPYILFIGMAALAMGILNCF
                     HVFGMPAATPILLNISFIIFSTAVVWRYFSSPAAALAVGVLVGGIFQFFLQVPQLVRK
                     GMNFEFAVSFTDPGVRRVARLMVPGFIGIGIAQINLLVDTVFANAKIMPEGSLISLYV
                     ADRVTELVLGGYAIAVATAILPMMSHQASAGDYEAMKRTFGFSLRIVSFITIPAAVGL
                     VILREPIVQVLFQHGLFVAESTRLTARALLYYSMGLPAFAAVKLIVPAFYSTQDTRTP
                     VRVAGIAMLANILLNVIFLFYFFAKFKNGGPALASALAGYLNVGILFVIFRLRFGRLG
                     TREIFTSVGKIALSAAIMGIVCWSTLRYSQFDMIEHFLPRLLVFICLIASAALTYLGV
                     AWLFRCAELSEVYGIAFHRESAIAGRTGWTG"
     gene            4511..5410
                     /gene="xerD"
                     /locus_tag="VG272_05320"
     CDS             4511..5410
                     /gene="xerD"
                     /locus_tag="VG272_05320"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HWF13136.1"
                     /translation="MDTTIHIFLTYVRVEKGLANNTILAYGRDLRRFDAFLARRRKCR
                     VEEVDREDVVDFLASLYKEKLDSRSVARYLVSLRNYFKFALMENLVRDDPTENLESPK
                     TRQSLPTYLRVDEVDKLLAAPDVSKPLGLRDRAMLEILYSSGLRVSELLNLRISDLDT
                     RLGCLRCIGKGDKERLVPVGRKAIAAVEQYLAVARPRFARPSSPPPHNQVLFLTRNGR
                     RLNRVSIWKIMHDYGMRLGLRGRLTPHKLRHSFATHLLERGADLRSVQLMLGHADIST
                     TQIYTHVVEERLKQVYKAHHPRA"
     gene            5518..>5960
                     /locus_tag="VG272_05325"
     CDS             5518..>5960
                     /locus_tag="VG272_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13137.1"
                     /translation="MPDYMYQLESRLSPEQRAALVRIQELAVANESNLYLVGGAVRDL
                     ISGMPIRDLDFAVEGNPTKIVRELEKGGAEVTGEVEALRCAELVMAGDVEVSLSAAHE
                     DVYPRPGAKPEIRFSTIMEDLKSRDFSINAIAISLNANSKGLLLDP"
BASE COUNT         1247 a         1580 c         1682 g         1451 t
ORIGIN      
        1 cgcaatttat cagatcactg aaatttccga cgtgaatgag aagcagcgtc gaatacgaaa
       61 tggcatattg cacggagtca ccgatcccac tcgatgattc gcacagccat tgtcgcagtt
      121 tttctgagcc tgtacaccct cgtgctggga ccgccgctga tcttgtatac gctgctcacg
      181 cggtcggcga acctgatgta ctgggtggga gtgaagggcg tcgtgttcat cacgcgtgtc
      241 gcgggcttgc gcgttcaagt ggaaggtata gaacacattc ccaggggcgt atgtatgttt
      301 gctgccaacc acaccagcaa tgcggatgcg gccgcgattg tgggtgcgat tcctcggcgt
      361 ctggcgattt ttggcagaaa atcacttttt gatattccca tcgtcgggct ggcattccgg
      421 ctggcgaaat tcgttcccgt ggatcgcggc aatcgcgacg cggcgctcgc ggccgtcaag
      481 caaggcgtcg aatacattaa gagcggatct tcatttcttg tctatccgga gggaacccgc
      541 agcccgaacg ggcgacttct acgcttcaaa aaaggcacct ttgtcatggc catcgaagcc
      601 ggcgttccga tcgttcccgt agcttgctct ggggcgcacc gggtgatgga gaaaaattcg
      661 ctggtgattc atccgggaaa agtaacggtg cgatttttag aaccgatcga cgcctcaaag
      721 tactcggtgg aacagagaga cgacctcgcg aaacgcgttc acgatctgat tgccgcgagc
      781 ttgccagagg atcagaaacc agcactccag gccgtactct aatcttcaaa tccaatttga
      841 aatctcaaat ttgggattcg agatttgaaa ttttggattt gagatcagac tatggcttca
      901 tcaagccatc aatgtctcgt ggctttgtgt ttgtagttta aatcttgtta tttcctggca
      961 gttttgattc ggtcgagctc ctctgcaaca tgacgcaatg ttttttgcat ttccggcagc
     1021 cgcttcagtg cggccatcag ctttagccag aatttatttt ccagattcgg atatccggaa
     1081 tgaatcgccc ccggcgggac ttccgaatgt acgccggttt ttgaagtaat tgtcgcgccg
     1141 tcgccgattt ccaggtgccc agcggacccg acctggcccg ccaggatgca acgattccca
     1201 acgcgtgtag atcctgcaag ccccacctgg ccgcagagaa tggaatcttc gccaacctca
     1261 cacgcgtgac ccacaagcac cagatcgtcg atttttgcgc cccggcgaat gcgcgtctcg
     1321 ccgacggtgg cgcgatcgat gcaggaattg gcctgaattt cgacgtcatc ctcgatgatt
     1381 accggagcct gctgcacaat cttgtgccag cggccatcgg cgcgcttggc aaaaccgaaa
     1441 ccgtcccctc cgattacgac gccgttctgt aggatcacgc gattgccgat gctgcagctt
     1501 tctctcacga ccacctgcga gtgtgccaga aaatcgtctc caattttggc gttccggtaa
     1561 attgtcacca ggctgtgcag gacggcgttg cgcccgattt ccacgccttc gtcgacgaag
     1621 cagtgggggc cgatgtgcgc tccttctccg attgttgcgg attttgccac cacggcggtt
     1681 gcgtgaatgc ccggcaagta cgtttgcggc gcgtgaaata gttccaccgc gcgcgcaaaa
     1741 tcaaagtacg gattgccgga acgcaggaca aataacgaag gcaaccctgg attgcgttta
     1801 acacctacct catttcccac gaacgccgcc gacgccagag tcgtggccag ttcccgagag
     1861 tacttgggat tggaaaggaa ggtgacctca cccgggcggg cgtctgcaat gcccgcgact
     1921 ccatggattt cggtctgcgg gtcgccttcg agcgtgcatc ccaatcgcgc ggccagttcg
     1981 ccgagtttca tctgatgatc tccttgaagg tacagttcgg caggctaaaa gcatagccgc
     2041 gcagtggacc gtccactgta catctttcaa atctggtttc acaaatgtcg cgtggtaaaa
     2101 gtcagacata gcaatcgcaa atttgaatct caaattcaaa cctgttcgag ttcacagtgt
     2161 gctgaaaact gaaaagccca accggaagtc tgttcactcg gagaaaagtg tcggttgggg
     2221 aggcgcagcc gatttccggc gggcaactcc cacgacgccg agcacggtca tttccgacag
     2281 ggcagtacgc gggatgaaga ttttgagtgt cgtaccatgc agatcgggag gagtaatctg
     2341 gacgccgcga tccaacagcg acagcaagtc attgggcacc acgccctcga tgctatcatt
     2401 atcgcggaat tttaggcgca cccacaggcc gtcgagcttg ggtctggaaa gaaacgcctt
     2461 gcgctcgggt tcaaaatcat cggtgaagtc gcgcacgaaa tagacgctgc gcacgtcctt
     2521 gaccgcgagg gtgcggtgcg ctccttcggg agtgagcacg tccagagcat cggcgccgcc
     2581 cagattgccg ggattcagat aaccgcgata tgtcgtgcgg tccagcaaca gcaccacgac
     2641 tttcttgagc gtggaggctg gggcgcgtaa tctcatggaa acggaagtag gattcagttt
     2701 aagccgcacg catgcagtgt agcgtgtaag cgcgtcaact cattccgtct gcaggccttg
     2761 cgggttaatg tgatctatgc tatggtaagc agtcttttgt tgtcaagtgt ttatttatca
     2821 gttatttagc ctgaggttca gactgacgcc ggttcgaatg aggctttgac tgggggcctc
     2881 gaccgcttga cccacattct gacccacaaa aagcagattc tcaagtctgc ttccattatt
     2941 agcatcatca cggtagtcag tcggattctc ggatacgtcc gtgaccagcg gctaacgctg
     3001 ctacttggga ctacgggtat tgccgattca ttcgttctgg cctacaggat tcccaatatc
     3061 ttgcggcgcc tggtcggcga gggatcgatg acggcctcgt ttattcctgt gtttaccgac
     3121 tacatgcgga atcgctccaa ggaagagacc tgggcgtttg cgaatcgatt gttttggaca
     3181 ttttcggtgg tcctggcggc gctgacggta ttgggcgtaa ttttttcgcc tctgatcgtg
     3241 cggatgttct cgctgttcgg aaagaatcaa gtccaggtgg aagcgatcta tctgaatcgc
     3301 ttgatgtttc cgtacatcct tttcatcggg atggcggcgc tggcaatggg gattttgaac
     3361 tgctttcacg tgttcggaat gcccgctgca acacccattt tgctgaatat ctccttcatc
     3421 atcttctcga ctgcggtggt gtggagatat ttctcaagtc cggccgccgc gctcgccgtg
     3481 ggcgtgctgg tgggcggcat attccagttt tttctgcagg tgccgcaact tgttcgtaag
     3541 ggaatgaatt ttgaattcgc tgtttcgttt accgatcccg gagtacgccg cgtggcacgc
     3601 ctgatggtgc ccggctttat cggtatcggg atcgctcaga ttaacctgct ggtggacacc
     3661 gtctttgcca atgccaaaat catgccggaa ggaagcttaa tttccctgta cgtcgccgac
     3721 cgcgtgaccg aactggtgct gggaggatat gctatcgccg tagccactgc gattcttcca
     3781 atgatgtcgc accaggcttc ggcgggcgac tatgaagcca tgaagaggac attcgggttt
     3841 tccctgcgga tcgtatcgtt tatcaccatt ccggcggcag tgggcctggt cattctgcgt
     3901 gaaccgattg tgcaggtgct gtttcaacat ggtttgttcg ttgctgaatc cacgcggttg
     3961 accgcacggg ctttgctgta ttattccatg gggctgcccg cgtttgcggc cgttaaactg
     4021 attgtgccgg cgttctattc gacgcaggat acacggacgc cggtgcgcgt tgcgggaatc
     4081 gcgatgctgg caaacatcct gctgaatgtg atttttcttt tctatttctt cgcaaagttc
     4141 aagaatggtg gtcccgcgct ggccagcgct ctggccggat atctgaatgt cggtattctc
     4201 tttgtaattt ttcgtttgcg attcgggcga ttgggaaccc gggagatctt tacatcggtt
     4261 ggaaaaatcg cattgtccgc ggccattatg gggatcgtat gctggagcac gctgcgctac
     4321 tcacaatttg acatgattga acattttctt cctcgcctgc tggtttttat ttgcctgatt
     4381 gcgtcggccg ccctgacgta tctgggggtg gcatggctgt tccgttgcgc ggaactcagc
     4441 gaggtctacg gcatcgcttt ccacagggag agtgcaatag cgggccgcac gggatggact
     4501 ggctagcgtg atggatacca ccattcatat ttttctcacc tacgtgcgcg ttgaaaaggg
     4561 cctggcgaac aacacaattc tggcctacgg gcgggacctg aggagatttg acgcctttct
     4621 ggctaggcgg cgcaagtgcc gggttgaaga ggtggaccgc gaagatgttg tcgattttct
     4681 cgccagccta tacaaagaaa agctcgatag ccgatccgtg gcgcgttacc tggtatcgct
     4741 tcggaattat tttaaatttg ccttgatgga aaatctggtg cgcgatgacc cgacggagaa
     4801 tctggaatct cccaaaaccc gccaaagcct cccgacatac cttcgcgtgg acgaggtaga
     4861 taaacttttg gcggctccgg atgtttccaa gccgctgggt ttgcgtgacc gggccatgct
     4921 ggaaatactt tattcctcgg gcttgcgcgt ttccgaattg ttgaacctgc gcatctccga
     4981 cctggacacc aggctcggtt gcctgcggtg cattggaaaa ggcgataagg agcgattagt
     5041 gccggtcgga cgcaaagcca tcgcggcggt cgagcaatat ttggctgtgg caaggccgcg
     5101 ctttgcccgt cccagcagtc caccgccgca caatcaggta ttattcctga ctcgcaacgg
     5161 aaggagactt aaccgggtca gcatctggaa gatcatgcac gattacggaa tgcggctggg
     5221 tttgcgcgga cggctcacgc cacataaact gcgtcacagt tttgccacgc acctgctgga
     5281 gaggggcgcg gacctgcggt ccgttcagtt aatgctgggc cacgcggata tttccacgac
     5341 tcagatatac acgcacgtgg tcgaggagcg cttgaagcaa gtctacaagg cgcatcaccc
     5401 gcgtgcgtga aaggccgacc accaatccac ggaatgcatc tcgtgggtta aaaaaaatcg
     5461 accgggtccg acgacactgt aaagaacaac ggtgtttcgc ccgaggacgg aatacgcatg
     5521 cctgattata tgtatcaact ggagagccgg ctgtcccccg aacaacgcgc ggcgctggtt
     5581 cgcatccagg aacttgccgt ggcgaacgaa tccaacctgt atctggttgg cggcgcggta
     5641 cgcgatctga tttccggaat gccgattcgg gacctcgatt ttgctgtgga gggcaatccc
     5701 acgaaaatcg tccgggaact tgaaaaaggc ggggcggaag tgaccggcga agttgaagct
     5761 ctgcgttgcg cggaacttgt catggccgga gatgtcgaag tcagcctttc cgcggcgcac
     5821 gaggatgttt acccgcgtcc cggagccaag ccggaaatcc gtttttccac cattatggaa
     5881 gatctgaaga gccgtgattt ttccatcaat gccatcgcga tttctctgaa cgcgaattcc
     5941 aaaggccttt tgctcgatcc
//
LOCUS       DASZMB010000116        14759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1764254, whole genome shotgun sequence.
ACCESSION   DASZMB010000116 DASZMB010000000
VERSION     DASZMB010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 14759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14759
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1764254"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..109
                     /locus_tag="VG272_05330"
     tRNA            24..109
                     /locus_tag="VG272_05330"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:57..59,aa:Leu,seq:caa)
     gene            405..884
                     /locus_tag="VG272_05335"
     CDS             405..884
                     /locus_tag="VG272_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWF13138.1"
                     /translation="MRIARIAAMAVFLAAPIFAHAQMAAAKNDKAVQEVVDFRNRYIE
                     AEENRDVAFLDKILADDFFALNPQGKLLDKAGQIENLKRPDRTLKVLNPRETHVQFYA
                     NGAVAVLTEHVTVDGMDKGRAFGGEYRFLRVFAKQNGNWKVVLAQGAPMPDQAAPAK"
     gene            complement(995..1723)
                     /locus_tag="VG272_05340"
     CDS             complement(995..1723)
                     /locus_tag="VG272_05340"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HWF13139.1"
                     /translation="MATPHFRRAEGTETFSPHMCSIPEGYFQMGCDAGRDDEKPAHRI
                     WVDAFELAAFQTTNSQYARFLENTARAPPSLWSDPDFNNPEQPVVAVSWFEADAYCQW
                     LSGLTDKKFRLPTEAEWERAARGGVEAEVFPWGDCSPENLPNYANRWKSGPEPVGLYA
                     PNAYGLFNMGDNVHEWCADWYDASYYLVSPERNPQGPSSSSRRASRGGSWRHHIKVTR
                     TAARSSIPPQLKYADYGFRVAASL"
     gene            complement(1735..2544)
                     /locus_tag="VG272_05345"
     CDS             complement(1735..2544)
                     /locus_tag="VG272_05345"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HWF13140.1"
                     /translation="MSSSQSSPTPYDSATGEKLIIETPEQTSIEFPLAGIGSRFLAVL
                     IDTLIQIGTVIVILLMFGGLGYSVRGAFGSIPDYLGPWIAAVLIFFSFLLMFGYFILF
                     ESLWNGQTPGKRLTHIRVIKDSGQPISAIDAVARNLLRLVDQLPFAYGIGVLCAWISP
                     QSKRLGDYVAGTVVVHEKPLENVTLQWESPAQNSAHHYGANRLTPEEFALIETFLSRR
                     SALEAGVRYDAAYGIVRRIESKLTLPPEGKPPAEKLLEELSWERRSTAGYH"
     gene            2547..3512
                     /locus_tag="VG272_05350"
     CDS             2547..3512
                     /locus_tag="VG272_05350"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HWF13141.1"
                     /translation="MISTRWLANRKQHWRRLEQLVDRSGRRGVAALDHDELQELGLLY
                     RQTASDLATVREDITSRPFTSYLNQLLARAHNLIYMGKRPDMSGIIRFYRDTYPRIFR
                     ETFPLVFLSTLIFIAGGIAGAVVMLRDPGFAHQILGPGMVETIEKREMWTHSILAVKP
                     MAASGIMTNNIMVGFMMFAAGISAGLGTIWMLITNGLLMGVLAVATARAGMALQLWDF
                     VAAHGALELPAIFIAAGAGLEIARGLLFPGILPRKDSLRLAGSQAAKLLLGTIPLLVV
                     AGVIEAFISPVKIATPLKFMLSAVLFTALVLYLLSGRKKIAVQET"
     gene            complement(3578..4975)
                     /locus_tag="VG272_05355"
     CDS             complement(3578..4975)
                     /locus_tag="VG272_05355"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWF13142.1"
                     /translation="MSSEKTFIPPAVIAQAHPKGRLGISFGPRFFVALLIGLVWVVPV
                     AWSPKLVAGIFIWDGLFLLLWLQDLLRLPKPDQLELQRIWHAPPSLGVRSKVSVELRN
                     FGSRAVFARVTDETSRALRRDPPVIQLAATAGGTASADYEILPAQRGDLPIGKFFVRY
                     QSALRLAERWAVAETAASVRVLPNLEQAKQQTLYLIRTRQVAMERRRRRLRGLGREFE
                     SLREYRTGDEFRNISWTATARRSKLITRVFQVERSQTVWIVLDAGRLLREQIVRGENS
                     LRLSKLDYAVDAALSLAQVAMHSGDRVGFLAYGRTIQQNRNAGRGAAHLHSIVESLAL
                     VHTEPYEADHSRAARALLTEQHRRSLIVWVTDLAETAGTPDVVEYALQMTRRHLVLFA
                     AIGQPDLNERVARRPESEEEMFRYIAAIEIVQRRELLLRRLRQRGVLAMEIMPGALAA
                     SLVNQYLDIKDRSLI"
     gene            complement(4972..5919)
                     /locus_tag="VG272_05360"
     CDS             complement(4972..5919)
                     /locus_tag="VG272_05360"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWF13143.1"
                     /translation="MENLAHLTTHIRTELSKVIVGQQDIVDQLLIVLACGGHALLEGV
                     PGLAKTLTVKTLARICALGFQRIQCTADLMPADIIGTNVFNLATSAFTLHRGPIFTDL
                     LLVDEINRTTARTQAALLESMEERQATIDGARYELGPHFTVFATQNPVEFEGTYPLPE
                     AQLDRFLMKIRIDYPSAEAETEILKRFETLTDAHLIEQTPLEPIDPALLAAARAEIAQ
                     VRVEPALYQYLVSVIRRTRDWPALSLGASPRAAIGLFFVARALAGMDGRDFLIPDDIK
                     NAAPAVLRHRLMLKPEADLEGVTADSVIAEILSAVEVPK"
     gene            complement(5920..7134)
                     /locus_tag="VG272_05365"
     CDS             complement(5920..7134)
                     /locus_tag="VG272_05365"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HWF13144.1"
                     /translation="MPPALDSGDRKLLLIAGSILLLLIIATLALAPSPEEAEGQGVST
                     TYSATTNGAQAAYLLLRELGYQSERWERPPTELPSDASGKILILAGPSDFPDKKERDA
                     LLTFARSGGWIVYAGNFPFLFLESGAVQTPSITRMSGLPSETFRAIAPSPFTQSAETI
                     AIKASNRWVTTDGAQVPLYGEPEEPVVVTWRLEKGRVIWWAAPTPVTNSGITRAGNLP
                     FFLSCIQAVRPGANPDEITVLWDEYFHGYRGSLWDYFSKTPVPWAIFQLAVVALFVLL
                     AFGRRSGPIYAPAAVSRLSPLEFVDTLGDLYRRANAGSAAVRVAYKHFRTKLLRRLAL
                     ASSVSNAELDSAVRDRLGWKQPGLMDILQRADKSSRENVTSNAEALKIVQALEHYEVL
                     FGLKTRPNEENR"
     gene            complement(7119..8174)
                     /locus_tag="VG272_05370"
     CDS             complement(7119..8174)
                     /locus_tag="VG272_05370"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HWF13145.1"
                     /translation="MPPERAPFFSSLLLRGLLLLVAILCVLHIVLADAPPATNSTAQS
                     PSNALYDSQSFTKQLGQLKAGLETARQSTETLRAYRESLPSSWTVNAGNHQFNVPTDL
                     LVSQLVKAERQPEIRTIKVKQALDYLDALALETMSVSADTPASTDSARAKLNAILSRP
                     EYAHAMQESWWGKIRSRINEMLGDALMRIFSSVGSQKSLGYILLWIAVCAAAVFIAYT
                     IFRRWIRAAKGAEMALQSMAVPLRSWQEWVFAAREAAARADYRMAIHCAYWAGIARLQ
                     ELGALSPDRAKTPREYLAALAKSKIILPETLATRQTALHTLTSRLEKIWYGYQIATEA
                     DVRDSLTQLETLGCRLP"
     gene            complement(8164..9045)
                     /locus_tag="VG272_05375"
     CDS             complement(8164..9045)
                     /locus_tag="VG272_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13146.1"
                     /translation="MATLDLRLLSLGELLDRTFFLYRRHFLLFVGISAIPYSFFFVAN
                     LATVLLPALARQANSGQIHSTGILAVVAGSGLLRVLALLIGCIAFLFSVGATVHAVSE
                     IYAGRATTIRESMASIRGESGTIFGVMFLSGLLLLAGLIAFVFPMFYIMCRVSVAIPS
                     AVLENLGPADSIRRSFTLTRNFAGRAFMIYLLYFAMVFGIIAVFQFPFAMLLVMSAKQ
                     PQLIVVWTILMQLGNFIGSALVAPVSTISFALFYYDLRVRKEAFDLQMMMQAIGPDPT
                     PPPVTGGVPSMFGRDAS"
     gene            9352..9771
                     /locus_tag="VG272_05380"
     CDS             9352..9771
                     /locus_tag="VG272_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWF13147.1"
                     /translation="MPKEKPKVNIGEVIRSYRGQRGLSQGDIERRTGLLRCYLSRVEN
                     GHTVPSLETLAKIAEAMEIALADFFPGAETPRDRDTQKMLGELSEDEIRFLSEIKKYS
                     TTLSDGDKRLVLAMIRKMATVIPPPPPRKIAARRTAY"
     gene            complement(10163..10576)
                     /locus_tag="VG272_05385"
     CDS             complement(10163..10576)
                     /locus_tag="VG272_05385"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HWF13148.1"
                     /translation="MTHGAKTKMEPGLEGRYERIVLQEWTLAHMDPNWPAVFTTPAMI
                     GMMEMASSYAVKPALPPGSITVGVRIEVDHFKPVPVGATVTAVSRLVEIDGRRLIFEA
                     EARSGEDVIGRGRVFHSIVDHTRFLAGARKKHAPR"
     gene            complement(10573..11766)
                     /locus_tag="VG272_05390"
     CDS             complement(10573..11766)
                     /locus_tag="VG272_05390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWF13149.1"
                     /translation="MREIAIVGGGPAGAMCGERLARYGYAVTIYDEHLAWEKPCGGGL
                     THKAIQAYPFLLNGPHPKKLIRSVELISSKGHRALLELDQPIVIYSRTVLNGLLLDRA
                     EAAGCRVVRSRVNQMNTSEKRANLSIGDSRVEADFVIVAAGARNSLLPGTSPLAPSDL
                     EMTLGYFVPAQAEGISVKFLSQFEGYIWSFPRCDHLSVGICGSMAKHTSQELRRHLHT
                     FMDGENISREGARFYSHVLPSPQQQTLSSRRVVGKNWALVGDAAAWVDPITGEGLYYA
                     LRSGELLAESIIADKPSEYPARIRKAFSTDLEFATRIARRFYRGNFLGGAVATRMIQF
                     IERSPTFRGLMADLFAGSQDYCGLKRRLWTQCGTTLTEVVGSLMKSQPSLAKNASAAG
                     SENLR"
     gene            12608..13276
                     /locus_tag="VG272_05395"
     CDS             12608..13276
                     /locus_tag="VG272_05395"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF13150.1"
                     /translation="MNRQITQPDGSRNPSRKRCEGFSVVELMIVVVIVLIVAAISIPG
                     FLTMIHNAKLQGAASDFGGLLQKARIRAVQDDVYYATYFTSSGTLLEAYVDVKKLVGS
                     TALDATVDPMVSMSSEVSVVAAGSAPNTSALQGLFLPTGSTLTVNDGASSSSPVIFNS
                     RGLPCTSISVSGGTLCSSSSTYTTAYWLFFQDNITHAWVAVTISPAGRIQKWRADGSS
                     WVKD"
     gene            13325..13888
                     /locus_tag="VG272_05400"
     CDS             13325..13888
                     /locus_tag="VG272_05400"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF13151.1"
                     /translation="MKRRTDRGTTLIELMIAMLVLAIGLGATTIMLTGAMASDNKSSK
                     DMTATLLAQMVMEQISAQHVYANTTINVTDCAGNSWTMATAAGDVGIGSGANLNSDGT
                     IDFTQSYSTLLSSNYAMQYVDCSTAGGAQTTYEVRWNVMAVSSNSATRLITSGARVMP
                     SDPTKLGGRMFAFPVTLRGIGAPSAGQ"
     gene            13916..14590
                     /locus_tag="VG272_05405"
     CDS             13916..14590
                     /locus_tag="VG272_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF13152.1"
                     /translation="MSALRQRGFSLVELMISLAILTIVIGVVMDGIMTMQKRNAVEVS
                     KVDLTQQAREFMDQIVNDIHQAGYPGLNMFDPATMNATCTNVADVNLACGLVSVTTST
                     LQFEGDVDGTGVSEVYIELIQPQGGCPCTLRRGTVTKAQYQAGTTPLFYTEVDNVMNT
                     AVFTAYLADGTQVTLPASAGNGLKGISAIGITLYVKASQPDLQTGQYPTVTMVATAKI
                     SNIQSL"
BASE COUNT         3503 a         3925 c         4119 g         3212 t
ORIGIN      
        1 gtgtcgtgta gaatctagga cgcgccggag tggtgaaatg gcagacgcgc tggactcaaa
       61 atccagtggc cgcaaggccg tgtgggttcg actccctcct ccggcaccac ctttttcttg
      121 aaccattttt ctattcaatc ggagttgagc tgcaacggcg cgggccgcgt gcagggcggc
      181 gattttttgg cattcaacca tagagtgttc actgtatttg ttggaagcgt tccggtcaat
      241 cgagcttgtt attttgatcc ggctggattg ccgccaactc ttttaggctg tcgtctgcta
      301 aaatccgtgc aagccacgca tacggcatct gccgccatgt gagattaatc tatggagagg
      361 gtgcgggtcc gccgatgcgg tagccgcaag aaagagagca ggaaatgaga atcgcccgaa
      421 ttgcggcaat ggccgtattc ctggccgcgc cgatctttgc tcatgcacag atggcggcgg
      481 ccaagaatga taaggccgtg caagaagtag ttgatttcag gaatcgatac atcgaagcgg
      541 aagaaaatcg tgatgtcgca tttctggaca aaatcttggc ggacgatttt tttgcgctta
      601 acccacaagg aaaattgctg gacaaagctg gccagatcga aaatctgaag aggccggatc
      661 gcacgctgaa agttttaaat ccgcgcgaga cacacgtgca gttttatgcc aatggagccg
      721 ttgccgtttt gacggagcat gtgaccgtgg atggtatgga caagggccgc gcttttggcg
      781 gagagtatcg cttcctacga gtattcgcca agcaaaatgg caattggaaa gtggtactcg
      841 cgcaaggggc tccgatgcca gatcaagctg cgccggcaaa ataaattttt attcagcggg
      901 gttgtgtggc ataggcttca gcctgtgtgg gtttaagcct tgcaaggact aaaaccgcac
      961 aggctgaagc ctgtgccaca ttaacttgca atcgctataa gcttgcggca acgcgaaaac
     1021 cgtaatcagc gtatttgagt tgcggaggga tgctggaacg tgccgcagtc cgagtcacct
     1081 ttatgtgatg gcgccacgaa cctccgcgcg aagcccggcg actgctggag gatggccctt
     1141 ggggattgcg ctcgggcgaa actaaatagt aagaagcatc gtaccaatcg gcgcaccatt
     1201 cgtgaacatt gtcgcccata ttaaaaagtc cgtatgcgtt tggcgcgtac aatcccaccg
     1261 gctcagggcc tgacttccag cgattcgcat aattaggaag attttccggc gagcagtcgc
     1321 cccagggaaa cacttcggct tccacgccgc cgcgcgcggc acgttcccat tcggcttccg
     1381 tgggtagtct aaactttttg tccgtaaggc cgctaagcca ttggcaatac gcgtcggctt
     1441 caaaccatga cactgcgaca accggctgtt ctggattatt gaaatcggga tcgctccaga
     1501 gcgaaggcgg cgcgcgggcc gtgttttcca aaaaacgagc atattgcgaa ttcgttgttt
     1561 gaaaagctgc aagttcaaat gcgtcgaccc agatgcgatg cgcaggtttc tcgtcatcac
     1621 gtcccgcgtc gcagcccatc tggaaatatc cttcggggat ggagcacatg tgtggtgaaa
     1681 acgtttctgt gccttcggct cgacgaaaat gtggtgttgc catagggatt ggtctcagtg
     1741 gtatccggca gtagagcggc gttcccagga aagttcttca agaagttttt ccgcaggggg
     1801 ctttccttcc ggcggtaaag ttaatttcga ttcgatgcgc cgcacgatgc cgtacgcggc
     1861 gtcataacga acgcctgctt cgagcgcgct gcggcggctg agaaatgttt cgatcagcgc
     1921 aaactcctcg ggcgtgagcc gattcgcgcc atagtggtgc gcggaatttt gcgcgggaga
     1981 ttcccattgc aaggttacat tttccaaagg cttttcatgc accacgaccg tgccggccac
     2041 gtaatcgccc agacgtttac tttgcgggct gatccatgcg caaagcacac cgattccgta
     2101 tgcaaaaggc aattggtcga cgagtctcag aagattgcgc gccacagcat cgattgccga
     2161 gatgggctgg ccggaatctt tgatcacgcg aatgtgcgtg aggcgtttgc ctggtgtttg
     2221 cccattccac agcgattcga acaaaatgaa gtatccgaac atcaggagaa aactaaaaaa
     2281 aattaggacc gcggcgatcc agggaccaag atagtcggga atgcttccaa acgccccacg
     2341 cacgctgtaa cccaagccgc caaacatcag taagataacg atcacagtcc caatctgaat
     2401 tagtgtgtca atcagcacag cgaggaaccg gctgccgatt cccgcaagtg gaaattcaat
     2461 cgaggtctgt tccggcgttt caatgataag cttttcgcct gtggcggaat cgtacggggt
     2521 tgggctggat tggctcgagg acataaatga tttcgactcg atggctcgca aatcgcaaac
     2581 agcactggcg ccggctcgaa cagctggttg accgatcggg gcggcgcggc gttgcggccc
     2641 ttgaccatga cgagttgcag gaacttggcc tattataccg ccaaacagcc tccgaccttg
     2701 caacggttcg cgaggatatc acgagccgtc ctttcacgtc ttatctcaat cagctactgg
     2761 cgcgcgcgca caatttaatt tacatgggca agcgcccgga catgagcggc atcatccgct
     2821 tctaccgaga cacgtacccg cgaattttca gggaaacgtt tccgctcgtt tttttgtcga
     2881 cgctgatttt tatcgcggga ggaattgcgg gcgccgtcgt catgctgcgc gatccgggtt
     2941 tcgcgcacca aattctcggg ccggggatgg tcgaaacaat tgagaagcgt gagatgtgga
     3001 cccattccat cctggccgtg aagccgatgg cggcgtccgg aatcatgacc aacaatatta
     3061 tggtcggatt catgatgttc gccgcgggaa tttccgcagg tctgggcaca atctggatgc
     3121 tgatcacgaa tggcctgctg atgggtgtgc tcgccgtcgc cacggctcgc gcgggcatgg
     3181 cgctgcagct ttgggatttc gttgccgcgc acggcgcgtt ggagctgccc gccattttta
     3241 ttgccgcggg agcagggctg gaaatcgcgc gcggcctgtt gtttccggga atactcccgc
     3301 gcaaggattc gttacggctg gcagggagcc aggcggcaaa gctgttgtta gggacaattc
     3361 ctttgctggt ggtagctggc gtgattgaag catttatttc accggtaaag attgcaacgc
     3421 cattgaaatt tatgctttcg gctgtcttgt ttaccgcgct ggttttatat ttgctgagcg
     3481 gcaggaagaa aattgcagtt caagagactt aaagattgta acagtcttca caaatcacca
     3541 atcacgaatt acgaaccgcg ctttcttcca ccgccgctca aatcaggctg cgatccttaa
     3601 tatcgagata ctgatttacg agtgaagcgg ccagcgcgcc gggcatgatt tccattgcca
     3661 ggacgccgcg ctgcctcagc cgccgcagca aaagttcgcg gcgctgcacg atttcgatgg
     3721 ccgcgatgta gcgaaacatt tcttcttcgg actcgggacg gcgcgccacg cgttcgttca
     3781 agtccggctg gccgattgcc gcaaaaagaa cgagatgcct gcgggtcatc tgcagtgcgt
     3841 attccaccac gtccggagtg ccggcggttt ccgcgagatc ggtgacccaa acgatcaggc
     3901 tccggcggtg ttgctcggtg agcagcgcgc gggccgcgcg gctatgatcg gcctcgtagg
     3961 gttcggtatg cacaagcgca agcgattcaa cgatggagtg caggtgcgct gcgccgcgtc
     4021 ccgcattccg attctgctga attgttctgc cgtaggcgag gaagccgacg cgatcgccgg
     4081 agtgcatcgc gacctgcgcg agggagaggg cggcgtccac ggcataatcg agtttcgata
     4141 atcgcagcga attttctccg cgcacgattt gctcgcgcag caggcgcccc gcatcgagca
     4201 cgatccatac ggtctggctg cgttccactt gaaacacgcg cgtaatcagc ttggaacggc
     4261 gcgccgtggc tgtccaactg atattgcgaa actcatcgcc agtgcgatat tcgcgcaagc
     4321 tttcaaattc gcggccgagt ccgcgcagcc tcctgcgccg ccgctccatg gccacctggc
     4381 gcgtgcgaat gaggtacagc gtttgttgct tggcttgctc aagatttggg agaacgcgca
     4441 cgcttgccgc ggtttcggct acggcccagc gttctgccag gcgtaatgcg ctttgataac
     4501 gcacaaaaaa tttgccaatc ggaaggtcgc cgcgctgcgc aggcagaatt tcgtaatccg
     4561 cgctggccgt tccgcctgcg gtcgcggcaa gttgaataac aggtggatca cggcgaaggg
     4621 cgcgcgaagt ttcatcagtc acgcgtgcga agacggcgcg gcttccgaag ttgcgcaact
     4681 ccacagaaac tttcgagcgc actccgagcg aaggcggagc atgccaaatg cgctgcaatt
     4741 cgagttgatc cggtttcgga aggcgcaaca aatcctggag ccacagcagc aaaaacaatc
     4801 catcccagat aaatattccc gcgactaatt ttggcgacca cgccacggga accacccaga
     4861 caagcccgat cagcagcgct acaaaaaatc tggggccaaa gctaatcccg aggcgccctt
     4921 tcggatgcgc ctgcgcgata acggccggag gaatgaaagt tttttcggag ctcacttcgg
     4981 aacctcgacc gccgacaaaa tttcggcgat gacggaatcc gcagtgacgc cttcaagatc
     5041 ggcttccggc ttgagcatca acctgtgcct cagcacggcg ggcgctgcat tcttaatgtc
     5101 atccggaatc agaaagtcac ggccatccat tcctgcgagt gcgcgtgcca cgaaaaataa
     5161 tccgatcgcc gcgcgcggac ttgcgccaag cgaaagcgca ggccaatcac gtgttcggcg
     5221 aatgactgaa acgagatatt gatacaaagc aggctcaacg cgaacttgcg caatttcagc
     5281 gcgagccgcg gcgagaagcg caggatcaat cggttcgagc ggcgtctgct caatcaaatg
     5341 tgcatccgta agcgtctcaa aacgctttaa aatttccgtt tcggcttccg cgcttggata
     5401 atcgatgcga attttcatca gaaaccggtc gagttgcgcc tcgggcaggg gataggttcc
     5461 ttcaaattcc acgggatttt gtgttgcgaa taccgtgaag tgcggtccga gttcgtagcg
     5521 agcgccgtcg atggtagctt gccgctcttc catcgattcg agcagcgcgg cctgcgtgcg
     5581 cgccgtggtg cgattgattt catcgacgag caacaagtcc gtaaaaatgg gcccgcggtg
     5641 cagtgtaaac gcgctggtgg ccagattgaa aacgtttgta ccaataatgt ctgccggcat
     5701 cagatcggcc gtgcactgaa tgcgttgaaa tccgagcgcg caaattcttg ccagcgtttt
     5761 caccgtaaga gttttcgcca ggccaggcac gccttcgagc agcgcgtggc cgccgcaagc
     5821 cagtacgatc aaaagttggt cgacaatatc ctgctgcccg acaattacct tcgagagttc
     5881 agtacgaata tgcgttgtga ggtgtgcgag attttccact tagcgattct cctcgttggg
     5941 tcgcgtctta agtccgaata aaacttcata gtgctctagc gcctgcacaa ttttaagagc
     6001 ctctgcattg gatgtgacgt tttcgcggga agatttgtca gcgcgctgca aaatatccat
     6061 gagccccggt tgcttccatc cgagccgatc acgcaccgcc gaatccaatt ccgcgttgga
     6121 aacagaagaa gccaaggcca gccgacgaag taattttgtg cggaaatgct tgtacgccac
     6181 gcgaacggca gctgaaccgg cattggcgcg gcggtacaaa tctcccagcg tatccacaaa
     6241 ttccagcggc gaaagccgcg ataccgcagc aggagcataa atcggaccgc ttcttcgccc
     6301 aaatgccaac aagacaaaaa gcgctacaac ggccagctga aaaatcgccc atggcacagg
     6361 cgtcttgctg aaatagtccc acagcgaacc gcgatagcca tgaaaatatt cgtcccacaa
     6421 aacggttatt tcatcgggat ttgcaccggg gcgcaccgct tgaatgcagc tgagaaaaaa
     6481 aggtaaattt ccggcgcgcg tgattcccga attcgtcacg ggagtgggag cagcccacca
     6541 gatcactcgt cctttttcca gccgccatgt aaccaccacg ggctcttccg gctcgccata
     6601 gagcggaacc tgcgcgccat cggtggtcac ccagcggttg gacgctttta tggcaattgt
     6661 ttccgcgctc tgtgtaaatg ggcttggtgc aatcgctcga aaagtttcag acggcaaacc
     6721 ggacatccgg gtgatggatg gggtctgaac agcgccgctt tcaaggaaaa gaaacggaaa
     6781 atttccggcg taaacaatcc atcctcccga acgcgcgaac gtcaacagcg catcgcgctc
     6841 tttcttgtca gggaaatcag aaggccccgc gagaattaaa attttccctg aagcatcgga
     6901 tggcaattcg gtgggcggcc tttcccagcg ctcggattga tagccaagct ctcgaagcag
     6961 aaggtaggcc gcttgcgctc cgttggttgt tgcggaatag gtagtggaaa caccctgacc
     7021 ctccgcttct tccggtgacg gggccagcgc aagggtcgca atgatcagca ggagcaggat
     7081 acttcccgcg attagcaaaa gtttgcgatc tcctgaatct agggcaggcg gcatcccaga
     7141 gtctccaatt gagttaagga atcgcgaacg tcggcttcgg tggcgatctg atagccgtac
     7201 caaatttttt cgaggcgcga ggtgagtgtg tggagcgccg tctgccgtgt cgcgagcgtt
     7261 tcaggaagaa ttattttcga tttggcgaga gcggccaggt attcgcgcgg agttttagcg
     7321 cgatcgggag aaagagcgcc gagttcctga aggcgcgcga tgccggccca atacgcgcaa
     7381 tgaatggcca tgcgataatc cgcgcgcgcc gccgcttcgc gcgcggcaaa cacccactct
     7441 tgccaggatc gcaaaggaac ggccatggac tgcaaggcca tttcggcgcc cttggcggcg
     7501 cgaatccagc ggcgaaaaat cgtgtaggca ataaacacgg ccgccgcgca cacggcaatc
     7561 caaagcaaaa tgtatccaag agatttctgg ctgcctacgc tgctgaaaat tcgcatcagc
     7621 gcatcgccca gcatttcgtt aatgcgtgat cgaattttgc cccaccacga ttcctgcatc
     7681 gcgtgagcat attcagggcg ggacaaaatg gcattcaact tagctcgcgc agaatccgtc
     7741 gaggccggtg tgtcggcgga aacggacatg gtttccaaag ccagcgcatc aagataatcc
     7801 agcgcctgct tcacttttat cgtgcgaatc tcaggctgcc tctccgcttt caccaattgc
     7861 gaaacaagca aatccgtagg gacattaaac tggtgattgc ctgcattcac cgtccacgac
     7921 gagggaaggg attcacgata agcgcgcaat gtttccgtgg actgacgggc ggtttccaaa
     7981 ccagccttca gctgccctaa ttgtttagtg aaggattggg agtcataaag cgcgttggac
     8041 ggggattgcg cggttgaatt cgttgcaggc ggcgcgtcgg ccagaacgat atgcagcacg
     8101 caaaggattg cgacgaggag caaaagccct cgcagcaaaa gggaactgaa aaatggagcg
     8161 cgttcaggag gcatctcttc caaacatgga gggcacgccc ccggtaaccg gaggaggagt
     8221 gggatcgggg ccaatcgcct gcatcatcat ttgcaaatcg aacgcttcct tgcgaacgcg
     8281 caagtcataa taaaacagcg cgaaactgat ggtgctcacg ggagccacga gcgcgctgcc
     8341 gataaaattt cccagctgca tcagaatcgt ccagacaaca atgagttgcg gttgtttggc
     8401 gctcatgacc agcagcatag cgaacggaaa ttgaaacacg gcaataattc caaacaccat
     8461 cgcgaaataa agcaggtaaa tcataaacgc tcgtccggca aaattgcgag tcaatgtgaa
     8521 actccggcgg atcgaatcgg ccggtcccaa attttcaagc actgcggagg gaatcgcgac
     8581 gctcacgcga cacatgatat aaaacattgg aaagacaaag gcgatcagcc cggcgagcag
     8641 caggagtcca ctcagaaaca tcactccgaa aattgttcca gattctccac ggatgctggc
     8701 catggactcg cgaatggtgg tcgcgcgccc cgcgtaaatt tcggaaactg cgtgaacggt
     8761 tgcacccacc gaaaaaagga aagcgatgca accgatcaaa agcgccagaa cgcggagcag
     8821 gccactgccc gcgaccaccg ccaaaattcc ggtggaatga atctgtccgg agttcgcttg
     8881 gcgagcgagt gcgggtagaa gaacggttgc tagattggca acgaaaaaga acgaataagg
     8941 aatcgcggaa attcccacga acaacaggaa atgccgccga tatagaaaaa aagtgcgatc
     9001 gagcaattca ccaaggctca gtagtcggag gtcaagcgtg gccatggggc ctcctggcgg
     9061 acgggacgcg caaagtgtgt gtactctatc aagaattgac cgcgagtcaa acgcttactg
     9121 tgttcttgat ccggtattgc ggatttcgtt cgagtaagcg tgaggaacgt attgtcttgt
     9181 ctggattgta ttcccgttgc atgcgcatgt tcgctcggca caatttatgc cgtgtcatct
     9241 gccggaacgg attacgaatt actaattgcg attcacagta atcaaagaaa ttacttgcac
     9301 acggatgctc cactgcgtaa gataccgccg ggtcaagaag gtgtggaacc cgtgcctaaa
     9361 gagaaaccaa aagtaaatat cggcgaagtg atccgcagtt accgcggcca gcgaggactt
     9421 tcccagggcg atatcgagcg gcgcaccggt ttgttgcgct gctatctctc tcgcgtcgag
     9481 aatggccata ccgtcccgtc tctcgaaact cttgcgaaaa ttgccgaggc catggaaatt
     9541 gctctggctg attttttccc gggcgccgaa actccgcgcg accgcgatac gcaaaaaatg
     9601 ctcggcgaac tttccgaaga cgaaattcgc ttcctctcgg aaatcaagaa atacagcacc
     9661 acgctttccg atggcgataa gcgcctcgtt ctggccatga ttcgcaaaat ggccactgtg
     9721 attcctcctc ctccgccgcg caagattgca gcgcgccgca ccgcgtatta gcttttactc
     9781 ctaatctcgt aaacacaaaa ttatttcgct caaaatctta ttgtccgcga ccgcttccag
     9841 tagcgcgggc ttcggcctgc ggggtttggt ccatgcagaa attaaacctc atctgttcga
     9901 cattttttgt acggacgaaa ccccacaggc tgaatagttt gcggaaaaag aagtttattc
     9961 tgtcattccg agcgaagcga ggaatctctc ttcgatttaa gtataagaaa ataaagaagg
    10021 agggattcct cgcttcgctc ggaatgacaa aatgttgatt ttttccgcag cctctgaagc
    10081 ccgtgctact taattccaaa acagcgctct tgcatactgg attgttttct ggaggaaatt
    10141 cttgagggga tatttcaggc catcaacgcg gggcgtgctt tttccttgcg ccggccagga
    10201 atcgagtatg gtcgacgatt gagtggaaga cgcgccctct accaatcacg tcttctccgc
    10261 tgcgcgcttc cgcttcaaag ataaggcgcc gcccatcaat ttccaccagc ctggacacgg
    10321 cagtgaccgt ggctccgacg ggcacaggct tgaagtggtc cacttcgatg cgcacgccca
    10381 cagtgattga cccgggcggc agagcgggct ttacagcgta cgaactggcc atctccatca
    10441 ttccgatcat cgccggggtc gtaaacacgg cgggccaatt cgggtccatg tgagccagag
    10501 tccattcctg caagacaatt cgctcatacc ggccttccag gccaggctcc atttttgtct
    10561 tggcgccgtg ggtcatcgca aattctctga gccagcggcc gacgcgttct tagccaggga
    10621 cggctggctc ttcatcaggc tccctacaac ttccgttagc gtcgtgccgc attgtgtcca
    10681 caatctccgc ttcaggccgc aataatcctg cgatccggca aacaaatccg ccatcagccc
    10741 gcggaaagtc gggctgcgtt cgatgaattg aatcatgcgc gtggcgacgg ctccgccgag
    10801 aaaattgccg cgataaaatc gccgcgcgat tcgagtggcg aattccaggt cggtcgaaaa
    10861 tgccttgcga atgcgcgccg gatattcgga gggtttatcg gcgatgatgg attcggcaag
    10921 caattctccc gagcgcagcg cgtagtaaag tccttcgcca gtgatcgggt ccacccaggc
    10981 agcggcatct cctaccagcg cccaattttt tccgacaacg cgccgtgaag aaagcgtttg
    11041 ttgctgtggc gaaggcaaaa cgtggctata gaaccgcgca ccctcgcgcg aaatgttttc
    11101 gccgtccatg aatgtgtgca agtgccggcg taactcctgg ctcgtgtgct tcgccatgct
    11161 gccacagatt cccacggaaa gatggtcgca gcgggggaag gaccaaatgt aaccctcgaa
    11221 ttggctcaga aatttaacgc tgatcccttc ggcctgtgcc ggcacaaaat agccaagcgt
    11281 catttccaga tcgctgggtg cgaggggact tgtgcccgga agcagggaat tgcgagctcc
    11341 ggcagcgaca atcacaaaat ccgcttccac acgcgaatcg ccaatggata agtttgcgcg
    11401 cttttcggac gtattcatct gattgacgcg cgaacgaacc acgcggcagc ccgccgcttc
    11461 cgcgcgatca aggagaagtc cgttcaacac ggttcgtgaa taaatgacga tgggttgatc
    11521 gagttccagc agagcgcgat ggcccttact ggaaataagt tcaacgctgc gaatcaattt
    11581 tttgggatgc ggtccgttga gcaggaatgg ataggcctgg atcgccttat gcgtgagccc
    11641 gccgccgcac ggcttctccc acgccagatg ttcatcgtaa atcgttacgg catagccgta
    11701 gcgagccagg cgttccccac acatggcgcc ggctggtcct ccacccacga tcgcaatttc
    11761 acgcatgctg agtcctcgac gaaaagggta gagcccggca cagtacctgg cgctggagat
    11821 gaaaggctca agtctgcgaa ggcacatgct tcacagcaag aaacctcggc gggagtatag
    11881 cagaaaatct caatgaaaca gcaggtgcgt ggaggaagtg gttctttcgc gagatcatca
    11941 tagtctagaa atagactggc gctgttgaca tcctggatgc ttcagaaagt ttcgcccctt
    12001 ttgtgtagcc ggaattgtag ctcgcttctt cagagacgag ggtttagatg aaagtgtgac
    12061 caacatttcc tgttcagcgc gtgtgccggg gttccaggcc gcggacctac cgtcggtaac
    12121 cttatttcag tctttgagta gatcatcgtc taaacccccg cctctgaaga ggcgggctac
    12181 agcacgccgg cagcggaaac ggggatgtca acagagccaa atagactcga aactttagat
    12241 ttgctcaatc taagaaaagt tttattttat atggacatcc gaacagactt acatcatttc
    12301 gttgcacctg attgtccgag cgccattctc tgcaaaggaa gtgagaggcc gtcgaagaag
    12361 tgcgggcgca aggttgtttt aagaccagat cataggcttc atttttggta acttttctat
    12421 ccaactagag cgagtcgatc gattttcagc gatccgcttc cacattaaat acctgaatca
    12481 gttacggatg cgtttgacgt tcaaatggtc tcactcctgc actaagtatt gcgtcgcagt
    12541 ggatgaggat ttgtcttcca ttccaacgaa ttgacttggc attttccatg aagcgcggcc
    12601 caattttatg aatcgtcaaa tcacccagcc tgacggaagt cgcaacccat cgcgaaaacg
    12661 atgcgagggg ttttcggttg tcgagctaat gattgtggtt gttatcgtct tgattgtggc
    12721 ggcaataagc atacccggtt ttcttaccat gattcacaat gccaagctgc aaggcgctgc
    12781 aagcgacttt ggcggactgc tccaaaaggc ccggattcgc gcggtccaag acgatgtcta
    12841 ctatgcgact tatttcactt cgtcgggaac cttactagag gcttatgtgg atgttaaaaa
    12901 actagttggc tcaaccgcgc tagacgccac ggtcgatccg atggtctcga tgtccagcga
    12961 agtttcagtc gtcgctgcgg gcagcgcccc gaacacgagc gctctgcaag ggctgttctt
    13021 gccgacgggt tccacgctga cggtgaacga cggcgcgtca tctagctcgc cggtcatttt
    13081 caattcacgc ggtctgccct gcacaagtat atcggtttct ggtggaactc tttgcagcag
    13141 cagttccact tacacgacag cgtattggct cttttttcag gacaatatca ctcatgcttg
    13201 ggtagccgtc acgatcagtc ccgcaggccg gatccaaaaa tggcgagcgg atgggagcag
    13261 ctgggtcaag gattgaagaa aggctttgcc taggatgagc ttgataacga ttcgcgaggc
    13321 acacgtgaaa cgcagaaccg atcgaggaac gaccttaatc gaactcatga tagcgatgct
    13381 ggtgctggca atcggcctcg gggcgactac gatcatgctt accggggcga tggcctctga
    13441 caacaagagc tccaaggaca tgacggctac cctgcttgcg cagatggtga tggagcaaat
    13501 cagcgcccag cacgtctatg ccaacacgac aatcaatgta accgattgtg ccggcaatag
    13561 ctggacgatg gcaaccgctg ctggagacgt ggggatcgga agtggcgcga acctgaattc
    13621 cgatggaact atcgacttca cgcagtccta tagtacgttg ctcagcagta actacgcgat
    13681 gcaatacgtg gactgctcca ctgctggagg cgcgcaaacg acttatgaag ttcgctggaa
    13741 tgtgatggcc gtttcgtcaa attcggcgac ccggctgatt acatcgggcg cgcgtgtaat
    13801 gccttccgac cctacaaagt taggcggacg tatgtttgcc tttcccgtga cgttgcgggg
    13861 aatcggcgcg ccgtcggcgg gacaataacg aaaaatggaa acgaggtccg gagaaatgag
    13921 cgccttgcga caacgcggat tttccttggt tgagttgatg atttccctgg cgattctaac
    13981 catcgtgatt ggcgtcgtca tggatgggat catgacgatg cagaagagaa acgcggtgga
    14041 agtcagcaag gtcgatctca cgcagcaagc gcgggagttc atggatcaga tcgtcaacga
    14101 cattcaccag gccggctatc ccggcttgaa tatgttcgat ccggcgacaa tgaacgcgac
    14161 ttgcaccaat gtcgcggatg tcaatctcgc ttgcgggttg gttagtgtga cgacgagtac
    14221 cctccagttt gaaggcgacg tggatggcac gggagtgagc gaagtttaca ttgaattgat
    14281 ccagccccaa ggtggatgcc cttgtacact tcggcgcggg acggtcacga aagcgcagta
    14341 ccaggcggga accacgccgc tgttctatac cgaggtagac aacgtgatga acacagccgt
    14401 attcactgcg tacttggccg atggcactca agtcacgctt ccagccagcg ctgggaacgg
    14461 attgaagggc atctcggcta tcggaatcac gctttacgta aaagcatcgc aaccggacct
    14521 gcaaacggga caatatccga cggtcacgat ggttgccacg gcaaaaatca gcaatataca
    14581 aagcttatag aaaaacggaa aaggagaact tgatccgcaa ggcgatatga cgatgaaact
    14641 caatgcagat aacagaggcc gattttatga ctgggggaac cggtccattg aagtcgatat
    14701 ctgcgaagtg aatcgtcggt tgtcacaaca cctggaaatg tttatcaggg gaaaacaag
//
LOCUS       DASZMB010000117         2720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3670598, whole genome shotgun sequence.
ACCESSION   DASZMB010000117 DASZMB010000000
VERSION     DASZMB010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3670598"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VG272_05410"
     CDS             <1..677
                     /locus_tag="VG272_05410"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWF13153.1"
                     /translation="ETNLINVTGTLNVLMAAREAKVKRVVFACTCAVYGKTSTLPIAE
                     NAALDPVSPYGVSKQLGEAYGRVFQDLYGLEFVSLRYFNVFGPRQDPGSPYSGVLSLF
                     NENLLRGTAPTVYGDGGQSRDFVYVENVVQANLLAAEIKSSPFQAINVGTGNRHTLNR
                     TLTLLEQITGHPAQANYAPARDGDIRESQADIRLAKAALGYHSRVTFEEGLKRTWQWF
                     SVNERA"
     gene            complement(719..1408)
                     /locus_tag="VG272_05415"
     CDS             complement(719..1408)
                     /locus_tag="VG272_05415"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWF13154.1"
                     /translation="MGDPITRFSEVRALIFDLDGTLIDSKLDLALAVNATLTKLGREP
                     LPHDQIFSYVGRGAPALISRVLGPAASEQDCMLGLEFFVKYYSAHKLDNTTLYPGVRE
                     TLDALKGMPMAVYTNKPVRVSRSIIEGLGVSEHFRYVYGGNSFERKKPDPMGVELILR
                     QFGAAPAQTMFVGDSDVDVQTARNSGTWVCGVTYGFGSHRLTDYPPDLLVDNLLELVP
                     YLSSSPLERSR"
     gene            1540..>2720
                     /locus_tag="VG272_05420"
     CDS             1540..>2720
                     /locus_tag="VG272_05420"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HWF13155.1"
                     /translation="MADIFPFRAYRYSAAVVDPAKVLTQPYDKITPAMAEKYAAASPY
                     NLIPIEKGKTLPSDTPANNVYTRAEKTLEEWIHAKVIVQDPAPSLYAYFQEYTVPGTS
                     ERRTRKGFIAVGRIEDYSAGVVFRHEQTLSGPKADRMELLRHTQTHTGQLFMLYNDPA
                     ARIDALLDQAANSKKPEVELRDEYDVVHRLWPITDTQMLETIRREMADKKLVIADGHH
                     RYETAMAYRDERRARDGRVDPNAPYEKVMMSVFNTVGKGLTILPTHRIVANLPNFSFE
                     KIRAALSNAFDVTSYAFQGSADHAQVYQQFRGDLLKGQAQRAIGAYAGDGAFHIFVLK
                     KDANLEELLKGVSPAQRQLDVVLLHRLIIERGLNITQDAVKTEKNITYEREMDTALAE
                     V"
BASE COUNT          690 a          725 c          722 g          583 t
ORIGIN      
        1 tcgaaacaaa tctcatcaac gtaactggca cgttgaatgt tctgatggcg gcacgggaag
       61 caaaagtaaa acgcgtggta tttgcgtgca cttgcgcggt ctacggaaaa acctcaacgc
      121 tgcccattgc cgaaaatgcc gcactcgatc ccgtttcgcc ctacggtgtt tccaaacagt
      181 tgggcgaggc ctatggacgt gtgtttcagg atttatacgg ccttgaattt gtctcgctgc
      241 gctattttaa tgttttcggc ccacggcagg atccgggttc tccttattcc ggcgtgctct
      301 ccttattcaa cgaaaatctg ctgagaggaa cagcgcccac tgtttatggg gatggcgggc
      361 aatcacgcga tttcgtctac gtggaaaatg tagtgcaggc aaatttgctc gctgcggaaa
      421 taaaaagtag cccgttccag gcgattaacg tcggcaccgg aaaccgccac accctgaatc
      481 ggacattaac attactagaa caaattactg gccatcctgc tcaagcaaac tatgcgcccg
      541 cgcgcgatgg agatatccgg gaatcacagg cggacatccg cctggcgaaa gcggcgctcg
      601 gctatcattc acgtgtcacc tttgaagaag gattgaaacg cacgtggcag tggttctccg
      661 taaatgaacg cgcttagatc cccaagtaaa cgggcttcaa atctaattcg caattttttt
      721 aacgggaacg ttccagcgga ctgctggaca aataaggaac aagttccagg agattgtcaa
      781 caagtagatc gggcggataa tccgtgagcc ggtggctgcc gaatccgtag gttacaccgc
      841 aaacccatgt accggagttg cgcgcggttt gaacgtcgac atcggaatcg ccaacaaaca
      901 tggtttgagc aggagcggcg ccgaattgtc gcaatattaa ttccacgccc attgggtccg
      961 gcttcttgcg ttcaaaactg ttgccgccgt acacgtaccg aaaatgttcg gagacgccga
     1021 gtccctcgat aatgctgcgg cttactcgca ctggcttatt cgtataaacg gccatgggca
     1081 tgcccttcag cgcatcgagt gtttcgcgca cgccgggata taaagttgtg ttgtcgagtt
     1141 tgtgcgcgga ataatacttt acgaaaaatt ccaggccgag catgcaatct tgttcggaag
     1201 cagcagggcc caatactctt gaaatcaatg ccggtgcgcc gcgcccgacg taactgaata
     1261 tctgatcgtg tggcaacggc tcccgcccta gcttcgtgag agtcgcgttg acggccaagg
     1321 ccaaatccaa tttggagtcg atcagcgtgc cgtccagatc gaaaatcaag gcacgcactt
     1381 cggaaaagcg agtgataggg tcccccatga atcttctgcg gcctcccgcg aaatattaat
     1441 tgtaatatag tcagttgagt ttcatacgag caaagtctcc gtgtagtctt ctttgaaatg
     1501 cgcgcggcgg catgtttata caaactggag gaagtaagaa tggcggacat ttttccgttt
     1561 cgagcttata gatacagcgc ggcagtcgtc gatcccgcga aagtcctgac acagccctac
     1621 gacaagatca cgccggcgat ggctgagaaa tatgctgcgg caagcccata caatttaatt
     1681 cccatcgaaa aaggtaagac acttccgagc gacacgccgg cgaacaacgt atacacccgg
     1741 gccgagaaga cgcttgagga atggattcat gcgaaagtca tcgtgcagga ccccgcacct
     1801 tcgctctacg cgtatttcca ggaatacacc gtgcccggaa cgagcgagcg ccgcacccgc
     1861 aaaggtttta tcgctgtcgg gcgcatcgaa gactattcgg cgggggtcgt gttccgccat
     1921 gagcagacgt tatccggacc gaaagcggac cgcatggaat tgctgcgtca tacccagacg
     1981 cataccggcc agctgttcat gctttataac gacccggcgg cgcgcatcga cgcgcttctg
     2041 gatcaagcag ccaactcgaa aaagccggaa gtggagcttc gggatgaata tgacgtcgtg
     2101 cacaggttgt ggcccatcac cgacacgcaa atgctcgaaa ccatccggcg cgagatggcc
     2161 gacaaaaaac tagtgatcgc cgacggccac catcgctatg agacagccat ggcttatcgg
     2221 gatgagcgcc gcgcacgcga cggtcgcgtc gatcccaatg caccttacga aaaagtgatg
     2281 atgtcggtat tcaatacggt cggaaaaggg ctgacgatac ttccaaccca ccgcattgtc
     2341 gccaatctcc caaatttctc gttcgagaaa atccgcgcgg ccctttccaa cgcattcgac
     2401 gtgacctctt acgcatttca ggggagtgcg gaccatgccc aggtttacca gcagtttcga
     2461 ggcgatttgc taaaaggtca ggcacaaaga gccattggcg cgtatgcagg cgatggtgca
     2521 ttccacattt ttgtgctgaa aaaggatgcg aatctcgaag aacttctgaa gggcgtttca
     2581 cccgcacaga ggcaactgga cgtcgtgctc ctgcacaggc tgatcatcga gcgaggcttg
     2641 aacatcacgc aggacgccgt aaagaccgag aaaaacatca cttatgagcg cgaaatggac
     2701 acagctcttg ccgaagtaga
//
LOCUS       DASZMB010000118         3593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2317950, whole genome shotgun sequence.
ACCESSION   DASZMB010000118 DASZMB010000000
VERSION     DASZMB010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2317950"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VG272_05425"
     CDS             <1..300
                     /locus_tag="VG272_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13156.1"
                     /translation="NGGKMARFDPKSQTFKEFQLPGPDPSPYAMGFDAEGYLWYDSHN
                     MDTINRFDPRTGRVIEYPVPHSELAMREFFRDAQGRMWYGTAPNNKVGYFYLAGK"
     gene            426..2366
                     /locus_tag="VG272_05430"
     CDS             426..2366
                     /locus_tag="VG272_05430"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HWF13157.1"
                     /translation="MNSNLDLLSPLRRYWGYDAFRPLQERIVRSLITGRDTCVIMPTG
                     GGKSLCYQLPAAMSEDKTVIVISPLIALMEDQVAQLGQMGIPAALLNSSITAAQQSRV
                     IENARTGAYRLLYLSPERLAREDTVEWLRGVPILFFAIDEAHCISEWGHEFRPDYRLL
                     SRLRAHFPDHAIAAFTASATQRVRHDIIRQLALRDPDKYIASFHRPNLRYVVRACDAS
                     SQTELLLRAVRSYGNENLIVYAPTIAKVESTVDFLQENGIPAIAYHGQMDAADRRRNQ
                     ERWMTDEVRVMVGTIAFGLGINKAAVRAVIHLSLPKSIEQYYQEAGRGGRDGLAADCV
                     LLWQKKDVGLHAYFIGQINDPQEKKRAWQRYDEIKGFVESPACRHQRICEHFGENKKW
                     AACGACDICGYQPEWLLAPVEEAPRKRKRSKSRPSALTSATVERTWERPTPRPAAARL
                     QIAEEADPNLREFIREWRKEIAKEQNIPAYVVMHDTTLDAICAVRPTSIARLLLITGI
                     GERKAELYGSQILYALKQFQEGARASAPDKKITPSEETIQLIEEGKSLEEIAMIRARR
                     LSTIVSMVSDLVERGLVDFRQDWVAENRRKQIENAAADLGLEKVTPIKNALPEDFTYD
                     EIRLVVSKLRRENKEKSFVAAT"
     gene            complement(2387..>3593)
                     /locus_tag="VG272_05435"
     CDS             complement(2387..>3593)
                     /locus_tag="VG272_05435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF13158.1"
                     /translation="ATTINYLDRAILGVILPEIRDKFHFGLPIYGWIQFFFQLAYAGG
                     SLLGGRMLDRYGTRIGYGLAATVWSLAATLNAIAGSAIQFGFFRIVLGFGESANFPAC
                     SKAAAEWFPPEERATAMGIVNAGTNLANIFGPPLFILIAFTLGWQACFAIMGGLGFLW
                     LPFWFLSYRLPKQEDAAAILGAKFSISEVMKYKQAWGYGLAKFLTDPVWWFYLFWLPT
                     YLTDVRHFTPQQRGTALMIVYSISAVGALMGGVVSSFLIKRGWTVGKARKMTLLFCAV
                     VMPACTLGVVVQDARLAVLLFGLATAAHQAWMTNLFIMPADVFPSQAVGSANGFGVCL
                     GGLGGALFSGIIPGTVIPHIGYVPVLMGMSCFYIIAWIIIHRMMGDLEMVTLGTDKTG
                     AVSLAPSRA"
BASE COUNT          912 a         1019 c          931 g          731 t
ORIGIN      
        1 aatggcggca aaatggcgcg attcgatccg aaatctcaaa catttaagga atttcagctg
       61 ccgggtccgg acccaagccc gtatgcgatg ggcttcgatg cggagggcta tctttggtat
      121 gactcgcaca atatggatac gattaatcgc ttcgatcccc gaaccggaag agtgatcgag
      181 tatccagtcc cgcattccga gctcgcaatg cgcgagtttt tccgagacgc gcaaggacgc
      241 atgtggtacg gcactgcgcc aaataataaa gtgggctatt tttatctggc tgggaagtag
      301 ggttgaggag cataggcttc agcctgtgga atttaggcct tgcaaagtct aaaacccaca
      361 gtctgaagcc catgctaccc actcaaagaa tggacccaac aaccgactag ctctatactc
      421 tccatgtgaa ttccaatctc gatcttcttt cgcccctgcg ccgttactgg ggctacgacg
      481 cgtttcgccc gctgcaggag cgcatcgtgc gcagcctaat tacgggacgc gatacgtgcg
      541 tcatcatgcc gactggcggc gggaaatctc tttgctacca attgcccgcc gcgatgtccg
      601 aggataaaac cgtcatcgtc atttccccct tgatcgcgtt gatggaagat caagtcgcgc
      661 aattggggca aatgggaatt ccggcggcgc tgctcaatag ttcgatcacc gcggcgcagc
      721 aatctcgcgt gatcgagaac gcccgcacgg gagcataccg cctgctctat ctatcgccgg
      781 aaagactcgc gcgcgaagac accgttgaat ggctgcgcgg tgtgcccatt ttattttttg
      841 cgattgatga ggcccattgc atttccgaat ggggtcacga atttcgtccc gattaccgct
      901 tgctcagccg tctccgcgct cattttccgg atcacgccat agcagccttc accgcgagcg
      961 ccacgcagcg cgtgcggcac gacattattc gccagcttgc gctgcgcgac ccggataaat
     1021 atattgccag ctttcaccgc cccaacttgc gctacgtcgt gcgggcctgc gatgcgagtt
     1081 ctcaaacaga acttcttctg cgcgccgtgc gaagctacgg aaatgaaaat ctgatcgttt
     1141 acgcgccaac catcgcaaaa gtagaatcca cagtggattt tcttcaggaa aacggaattc
     1201 ccgcgattgc ctatcacggc cagatggacg ctgccgatcg caggcgcaat caggaacgct
     1261 ggatgacgga cgaagtccgc gtgatggtcg gcactatcgc gtttggcctg ggcatcaata
     1321 aagcagcagt gcgcgcggtg attcatctct ccttgcccaa atccatcgaa cagtattacc
     1381 aggaggcggg ccgcggcggg cgcgacggcc ttgccgcgga ttgcgtgctt ctatggcaga
     1441 aaaaagacgt aggcctgcac gcttacttta tcggccagat caacgatcct caggaaaaaa
     1501 aacgcgcctg gcagcgctac gacgaaatca agggcttcgt tgaatcaccc gcttgccgcc
     1561 accagcgcat ttgcgaacat ttcggcgaaa ataaaaaatg ggctgcatgc ggcgcgtgcg
     1621 atatttgtgg ctatcaacct gagtggctat tggcccccgt tgaggaagct cctcgcaaac
     1681 gaaagcgctc caaatcgcgt ccctccgcgc tgacatccgc aacggttgag cgaacctggg
     1741 aaagacccac gccccggccg gccgcggcgc gattgcaaat agcggaagag gccgatccga
     1801 atttgcgaga gtttatacgc gagtggcgaa aggaaattgc taaagagcaa aatattccag
     1861 cctatgtcgt gatgcatgac acgacgctcg atgctatttg tgcagtccgt cccacgtcaa
     1921 tcgctcgcct tttgctgata accggtattg gcgagcgcaa ggctgaattg tacgggagcc
     1981 aaattctata cgcgctgaaa cagtttcaag agggagcacg cgcttctgcc ccagataaaa
     2041 agatcacacc aagcgaagaa acaattcagt tgatcgaaga agggaagtcg ctggaagaaa
     2101 ttgcgatgat ccgcgcccgc cgactttcga cgattgtcag catggtttcg gatttagtgg
     2161 agcgcggcct ggtggatttt cgtcaggatt gggtcgctga gaatagacga aagcaaattg
     2221 aaaatgccgc cgccgacctc ggcctggaaa aagttacgcc aatcaagaac gcactgccgg
     2281 aagatttcac ttacgatgaa attcgcttgg tcgtctcaaa attgcgccga gagaataagg
     2341 agaaatcgtt tgttgcagca acgtaattta gggcgtcagc catggattaa gcgcgtgatg
     2401 gcgctaacga gaccgcccca gtcttgtcag ttccaagcgt aaccatttca agatcgccca
     2461 tcattcgatg aatgatgatc cacgcaataa tataaaagca actcatcccc atcagcaccg
     2521 gaacgtaccc gatgtgcggg atcacggttc cgggaataat tccggaaaat agcgcgccac
     2581 ccagccctcc caggcaaact ccaaaaccat tcgcgctgcc caccgcctgc gaaggaaaaa
     2641 catccgcggg catgatgaat aaattcgtca tccaggcctg atgagccgcg gtcgccaagc
     2701 caaacagcag cacagccaat cgcgcatcct gcactacgac gcccagcgtg caggctggca
     2761 tgaccaccgc gcaaaaaagc agagtcatct tgcgtgcttt gcccaccgtc cacccgcgct
     2821 tgatcagaaa actggaaacc acgccgccca tcaacgcgcc aaccgcggaa atgctgtaca
     2881 cgatcatcag cgccgtaccg cgctgttgcg gcgtaaagtg gcgcacatcc gtgagatatg
     2941 ttggcaacca gaataaatag aaccaccaga ctggatcggt caaaaatttc gcgaggccat
     3001 atccccaagc ctgtttatat ttcatcactt cgctgatgga aaattttgcg ccgagaatcg
     3061 ctgcggcatc ttcttgtttc ggcaatcggt acgacagaaa ccaaaaaggc agccacaaaa
     3121 atcccagccc gcccatgatc gcgaagcacg cctgccatcc cagcgtaaat gcaatcaata
     3181 tgaaaagcgg cggcccgaaa atatttgcca gatttgtgcc cgcgttcacg attcccattg
     3241 cagtggcccg ttcctcgggc ggaaaccatt ccgcagcggc cttgctgcat gccggaaaat
     3301 tagcggattc accgaagccc agaacaatgc gaaagaatcc aaattgaatc gcacttccag
     3361 cgattgcatt caacgtcgcg gcaagggacc acacggttgc cgccaagccg tacccaattc
     3421 gtgtgccata ccgatcgagc attctgccgc ccagcagcga tccgcccgcg taagcaagct
     3481 gaaagaaaaa ctgaatccag ccgtagattg gcaatccgaa atgaaatttg tcgcggatct
     3541 ccggaagaat cacgccgaga atcgcgcggt ccaaataatt aatggtagtg gca
//
LOCUS       DASZMB010000119         6791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3135761, whole genome shotgun sequence.
ACCESSION   DASZMB010000119 DASZMB010000000
VERSION     DASZMB010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6791
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3135761"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="VG272_05440"
     CDS             complement(<1..229)
                     /locus_tag="VG272_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HWF13159.1"
                     /translation="MPVDNILRGLKRFRKKVYPKHRELFHKLALQQRPTALFITCADS
                     RIDPCLLTQTKPGELFLCRVIGNIVPRYPESI"
     gene            1113..2720
                     /gene="rpoD"
                     /locus_tag="VG272_05445"
     CDS             1113..2720
                     /gene="rpoD"
                     /locus_tag="VG272_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HWF13160.1"
                     /translation="MPAVEPTPEPEDGEHHLSGFEQIGVAGEEDAAAGGAALAIPDAA
                     EGDHGAVAEEGELDLTPGMLDKNNDPVRLYLREMGTVPLLKRADEVAIAKRMERGHLM
                     VLKTISRSPIVLKELIAVGRDLRAGTLSIKKIIQFDQEELTEEETESKTQEILRIIAK
                     IEQLYALAHKQAAQLESTPKSNKKLYLRYRNRLARTRVEMSQHARSIDFHSLERKRLI
                     DKIHSTVERLQSLEREIVKLERRADASKGDHAAEARRELRNRRAELKEIEAASETGLV
                     DLKRMLQVVLRGEAEASQAKKEMVEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMK
                     GADKFEWRRGYKFSTYATWWIRQAVTRAIADQARTIRVPVHMIETINKQVRTSRQLVQ
                     ELGREPRAEEIAHRLGVPVEKVRKTKEIAQQPISLETPIGEEEDSHLGEFIEDKSSVS
                     PSDAAISISLQEQTESLLKTLTPREERVIKMRFGLEDGSEHTLEEVGQSFAVTRERIR
                     QIEAKALRKLRHPSRSRNLRSFLEDSI"
     gene            complement(2804..2995)
                     /locus_tag="VG272_05450"
     CDS             complement(2804..2995)
                     /locus_tag="VG272_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13161.1"
                     /translation="MNPGTQVIAPKKGDHVAIAQQEGVFEVADINSLMQTANLKLTDG
                     LGHITRNVPWTDLKPVTKK"
     gene            3174..3650
                     /gene="rpiB"
                     /locus_tag="VG272_05455"
     CDS             3174..3650
                     /gene="rpiB"
                     /locus_tag="VG272_05455"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HWF13162.1"
                     /translation="MSNPSSKPRMIVGSDHVGFRAKENIKKYLEGAGYTVEDVGTHSE
                     ESVDYPDFAKAVGERVAAANGALGVLVCGTGIGVSIAANKIEGIRAALAHDSLTAQRA
                     REHNDANVIALGGKVVGEDEAIAIVQEFLAAEFAGGRHQRRIDKISEMDQVRERQS"
     gene            3874..5187
                     /gene="glyA"
                     /locus_tag="VG272_05460"
     CDS             3874..5187
                     /gene="glyA"
                     /locus_tag="VG272_05460"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF000586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HWF13163.1"
                     /translation="MDTKTSEHMNRSLREVDPDIAEVLREEGRRQGTGLELIPSENFV
                     SEAVLEAMGSVLTNKYAEGYPGKRYYGGCEFVDRAEQLAIDRAKALFGAEHANVQSHS
                     GTQANVSVYMSALQLGDTVLGMNLAHGGHLTHGHPLNFSGKMYKFIPYGVSKETETID
                     YDELGRLAHEHKPKMIVAGASAYSRIIDFPRMAQIAKEVGALFFVDMAHIAGLVAGGV
                     HPSPVPHADFVSTTTHKTLRGPRGGLVLCKQQFAKELDRITFPGTQGGPLMHTIAAKA
                     VCLKEAAEPGFREYAKQIVANAQKLAAEISKAGFRIVSGGTDNHLFLIDLYSRGITGK
                     DAQPALDRAGITTNKNSIPFDPTPPMTAGGIRMGTPAITTRGMLEKDMPKIAAWIADV
                     LLHLGDAERERRIRSEVAEFAAQFPLYTKRWDIAPSFAGSSGSAR"
     gene            5200..6348
                     /locus_tag="VG272_05465"
     CDS             5200..6348
                     /locus_tag="VG272_05465"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HWF13164.1"
                     /translation="MRVAIDIRRAGDFGIGTYIRNIINQFARQSDETEYILIGQQSHL
                     KQFDPLPENFTLLECLANPGSFETHFYLPYKLHKLGVDLLHMPWFYAPAVVPFRLVIT
                     IHDLTDILTPPPGISAPFQAGRLYFARRALTRADRIIAVSHSSKRDLSRAFDVSEDKI
                     EVVYNALDERFVREPMPEGADRILERHAVIDPFVLYAGNIKPQKNLPRLIEAFAVAKS
                     ELRDHPKYANLKLLLIGDSAEEHSDLRRAVLRSRVQSDVRFLGFVPHPVLRVFYSRAS
                     AFLFPSLYEGFGLPPLEAMAHGAPVLTSSVSSLPEVFSQAALLVNPENIFEIARGIRQ
                     ILTEDVLRETLVRRGYELVQRYSWERSAAQVREIYRSVVDGEPARVTS"
BASE COUNT         1622 a         1818 c         1899 g         1452 t
ORIGIN      
        1 cgatcgattc cggataccga ggcacgatgt tcccgatcac acggcaaagg aacagctcgc
       61 ccggcttggt ctgcgtcagc aggcaagggt caattcggga atcggcgcag gtgatgaaca
      121 gagcggtggg cctttgctgc aaggccagct tgtgaaatag ttcacggtgc ttcggatata
      181 ccttcttccg gaagcgtttc agaccccgaa gtatgttgtc gacaggcatg ggaggccatt
      241 ctgcatcagt atggagctgg gcatctataa aaatctggga ccatctggaa ctcttttgtg
      301 gtaaggtatc gcgctcgcag gtcgatggga aaaggctcgg gagcgagcag gagtcggcta
      361 gatcgacagg tcgatccacc ttaggaatcg aacagtcacc cctgggccga gtggtgtcct
      421 aatttgaaaa gaattcgcgc ggcacggttc cttaggaagt cgggacttca gtcccgacga
      481 gaagcttggt ttgatcgggg ttttaacccc tgaagcttca ggggctgaag cccgcgacga
      541 aaggcaaccc aaatggccga gttgaaactc ggccctccta aattcgagta attttcaaat
      601 taggacacta cctgggccga tcttgcgtgc aagaaatgca aaatcgtgag atactaagtg
      661 ttccagttat gaattctaat ttcggctctt aaggggttca gcggccgtgg caggcttctg
      721 aaacatactt ttttcgttct aatccgtcga aagttattag ttgcagttgg cgtggtgcgg
      781 atcaaggatc cctggttctt atcgcgtctt agcccgctac acattattga aaagaaatga
      841 gataaaacta cgtcgattcg ccggggccgg tctgcgttac cgggggcctc gaatcgactc
      901 taatggatag agaatcaaat cggctcgggc gcgcatcaaa ctgtgacacc tgtcgatagt
      961 acttgtgcgg taagaagatc gaactgagtt aattcgcaag ggggataacg gcattgcctc
     1021 tgaataatca cgaatcgggt gatgaggtcc ggagcatcat cgggatcggt aaagagcgca
     1081 ggtacctgct acctgacgag gaagaaagtg ccttgcctgc cgtcgaaccc actccggagc
     1141 cagaagacgg agaacatcat ctctccggct tcgaacaaat tggagttgcg ggcgaggaag
     1201 atgctgccgc cggaggcgca gcactggcta ttcctgatgc cgccgaaggt gatcatggcg
     1261 cagtcgcgga agaaggtgaa cttgacctga ctccgggaat gctggacaag aataatgacc
     1321 cagtccgcct ttatctgagg gagatgggca ctgttcctct gctaaagagg gcggatgaag
     1381 tggccatcgc caagcgcatg gagcgcgggc atctgatggt cctgaaaaca atttccaggt
     1441 ctccaatcgt tctgaaggaa ttgattgcgg tcggccgtga cctgcgcgcc ggcacgcttt
     1501 ccatcaagaa aattattcaa ttcgatcagg aagaactgac cgaagaggaa accgaaagca
     1561 agacgcagga aattctgcgt atcatcgcga agatcgaaca actttacgct cttgcccaca
     1621 agcaggccgc gcaactcgag agcacgccga aatcgaacaa gaagctttac ctgcgctatc
     1681 gcaaccgtct tgcccgaacg cgcgtcgaga tgtcacagca tgcccgttcg attgatttcc
     1741 actcgctgga acgcaagcgc ctgatcgaca aaattcacag cacggtcgag cgtctgcaat
     1801 cgctggagcg tgaaatcgtc aagctcgagc gcagagcaga tgcttccaag ggcgatcatg
     1861 ccgccgaggc acgccgggaa ctgcgcaatc gccgggcaga gctgaaggaa attgaagcag
     1921 ccagcgaaac cggattagtg gacctgaagc gcatgcttca agtcgtgctt cgtggagaag
     1981 cggaagccag ccaagccaag aaagaaatgg tcgaagcgaa cctgcgcctt gtggtttcca
     2041 ttgccaagaa atataccaat cgcggattgc agtttctgga tctgattcag gaaggcaaca
     2101 tcggcctgat gaagggtgcg gacaagtttg aatggcgccg cggctataag ttttccacct
     2161 atgctacatg gtggattcgc caggccgtga cccgcgccat cgccgatcag gcccgcacga
     2221 ttcgcgtgcc cgtgcacatg atcgagacca tcaacaagca agtgcggacc agccgccaac
     2281 tcgtccaaga gttgggacgc gaaccgcgcg cggaagaaat cgcacatcgc ctgggcgtgc
     2341 ctgtcgaaaa ggtgcgcaag acaaaggaaa tcgcgcaaca accgatttcg ctcgaaactc
     2401 caatcggcga agaagaagat tcgcacctcg gcgaattcat cgaggacaaa tcttcggtgt
     2461 ctccgtccga cgcagctatc agcatcagcc tgcaggagca aacggaatcc ctgctgaaaa
     2521 ctctgacgcc gcgcgaagag cgagtgatca agatgcgatt cgggctggaa gatggttcgg
     2581 aacacacact ggaagaagtg ggacagtctt tcgctgtaac ccgcgagcgc attcgccaga
     2641 tcgaagcgaa ggcgctgcgc aagctgcgac acccctcccg atcgagaaat ctccgctcat
     2701 tcctcgaaga cagtatctaa gctcgtctgc cgaggctcaa gaacctaagt agctggaatg
     2761 cctgaggcat ttcggctgct tttttccgca gcctcaaatc ttgctacttc ttagtgacag
     2821 gtttcaagtc cgtccacggc acattgcgcg tgatgtgccc tagtccatcc gtcagcttaa
     2881 gatttgctgt ttgcatcagc gagttaatgt cagcaacttc aaacacacct tcctgttgcg
     2941 cgattgccac gtgatcgcct tttttcggag caatcacttg agttccggga ttcatttggt
     3001 cctcctggat tactgttcta actgtcagaa atcgggccgc tggggaggaa tatctactga
     3061 ggatattctt acgcctagtt gaactgaatt tccactacta tcattttccg ggttctgctt
     3121 tgcctggttg gaatatcaga gcggattccg atagaatcat cgaggataat tcgttgtcaa
     3181 acccctcctc caaaccgcgc atgatcgttg gctccgatca cgttggattt cgcgcaaaag
     3241 aaaacatcaa gaaatatctc gaaggtgcgg gctacacggt ggaggatgtg ggcacgcatt
     3301 cggaagaatc ggtcgactac cctgacttcg caaaggcagt gggcgagcga gtcgccgccg
     3361 cgaatggcgc gctcggcgtg ctggtatgcg gcacagggat cggcgtctcc attgcggcaa
     3421 ataaaataga aggcattcgc gcggcgctgg cgcatgattc tctcactgcg caacgcgcac
     3481 gcgaacacaa cgacgcgaat gtgatcgcgc tgggcggcaa agtagtgggc gaggacgaag
     3541 ccatcgccat cgtgcaggaa tttcttgccg ccgaatttgc cggcggacgc catcaacgtc
     3601 gtatagataa gatcagcgaa atggatcagg tacgagagcg ccaatcgtga ccggcgcgga
     3661 atttatttag tcttgattgc attcccggtg acataaacga aatcggacgt cgtaagcctt
     3721 gtagctcgcc tctctcgtcc tgagtgaaaa cgaaggatcg gagccggggg cttagatgac
     3781 gtaacatcta aacccccggc tctgaagagg cgggctacaa cggcgcgcta ccataaatat
     3841 ggaaattcag tcgtcgaggt cgaaacaaaa taaatggaca ccaaaacatc agaacacatg
     3901 aatcgctctt tgcgagaagt cgacccggat atcgcggaag ttttgcggga agaaggccgc
     3961 cgccaaggca ctggcctcga gctgatcccc tcggaaaatt ttgtatccga agctgttctc
     4021 gaagccatgg gctcggtcct aacaaacaaa tacgcagaag gctatccggg aaagcgttac
     4081 tacggcggct gcgaatttgt cgaccgcgcc gagcagctgg cgattgaccg cgcgaaagct
     4141 ttatttggcg cagagcacgc caacgtgcaa tcgcactccg gcacgcaggc aaacgtttcg
     4201 gtctacatgt ccgcattgca actgggcgat accgtcctcg gcatgaacct cgcgcacggt
     4261 ggccacctga cgcacgggca tccgctgaat ttttccggaa aaatgtacaa atttattcct
     4321 tacggcgtaa gcaaggaaac cgagaccatt gattacgacg aactcggccg gctcgcgcac
     4381 gagcacaagc cgaagatgat cgtcgcgggc gcgagtgcgt attcgcgcat catcgatttc
     4441 ccgcgcatgg cccaaattgc aaaagaagta ggcgcgctat tttttgtgga tatggcgcat
     4501 atcgcgggac tcgtcgcagg cggggtgcat ccaagtcccg tgccgcacgc cgattttgtt
     4561 tcgaccacta cgcacaaaac gctgcgcggg ccgcgcggcg gactcgtcct ttgcaagcag
     4621 caatttgcaa aagagctgga ccgcattact ttccccggca cacaaggcgg accgctgatg
     4681 cacaccatcg cggcgaaagc cgtgtgcttg aaggaagcgg ctgagcccgg cttccgcgaa
     4741 tacgcgaaac aaattgtggc caatgcgcaa aagcttgcgg ccgaaatctc gaaagcgggt
     4801 ttccgcattg tcagcggcgg cacggacaat cacctgttcc tgatcgattt atattcgcgc
     4861 ggaattaccg gcaaagacgc gcaaccggcg ctcgatcgcg cgggtatcac caccaataaa
     4921 aattcgattc ccttcgatcc gactccgccc atgacggcag gcggaattcg catgggcaca
     4981 ccggcgatta ccacgcgcgg aatgctcgaa aaagatatgc cgaaaatcgc ggcgtggatc
     5041 gcggacgtac ttttgcacct gggcgatgcc gaacgcgaac ggcgcatccg ctcagaagtc
     5101 gcggagtttg cggcgcagtt tcctctttac acaaagcgat gggacattgc tccgtccttc
     5161 gcggggagca gcggctccgc gcgctaagcg ccgccacgga tgcgtgtcgc gatcgatatc
     5221 cgacgagccg gggatttcgg aatcggcacg tacattcgca acatcattaa ccaatttgcc
     5281 cgccagagcg acgaaacaga atatattctg atcggccagc aatctcattt gaagcaattc
     5341 gatccgctgc ccgaaaattt tacgctgctt gaatgcctag ccaatccggg atccttcgag
     5401 acacactttt atctcccata taaattgcac aaattgggcg tcgatcttct gcacatgccc
     5461 tggttctacg caccggccgt ggttcctttt cgtctcgtca tcactatcca tgacctgacg
     5521 gatattctca ctcctccgcc gggaatttcc gcccccttcc aggctggccg tttatatttc
     5581 gctcggcgcg ccctgacccg cgcggaccgc atcatagctg tgtcgcattc ctcgaagcgc
     5641 gatctttcgc gggcgtttga cgtgtcggaa gataaaattg aagtcgtgta taacgcgctc
     5701 gacgaacgat ttgtgcgcga gccaatgccg gagggcgcag accgcatcct ggaacgccac
     5761 gccgtcatcg atccgtttgt gctgtatgcg gggaacatca agccgcagaa aaatctgccg
     5821 cgcttgatcg aagcattcgc tgtggccaaa tccgagctgc gcgaccatcc taaatatgca
     5881 aatttaaaat tgctgctgat tggcgattcg gccgaggaac attccgatct gcggcgcgcc
     5941 gtgcttcgtt cgcgcgtgca atccgatgtt cggtttcttg gattcgtgcc gcatcccgtg
     6001 ctgcgcgttt tttattcccg agcgagcgcg ttcctcttcc cttcgttgta cgagggattc
     6061 gggctgcctc cgttggaggc tatggcgcac ggcgcgcccg tgctgacttc cagcgtatcg
     6121 tcgctgcccg aagtattttc gcaggccgcg ctgctcgtaa atcctgaaaa tatttttgaa
     6181 attgcgcgcg gcatccggca gattctcact gaagacgtgc tgcgcgaaac gctggtgcgc
     6241 cgcggctacg aacttgtgca gagatattcg tgggaacggt cggcggcgca agtgcgggaa
     6301 atctaccgct ccgtggtcga cggagagcct gccagagtta cgtcataagt tcaaaagatt
     6361 tgacacggag ggcacagagg aaaaaaaaga gaaaacaaac ttccggttct gtcctaattt
     6421 gaaaagaatt cgcgtggcgc ggttccttag gaggtcggga cttcagtccc aacgatgagc
     6481 ttgggttggt cagggcttta acccctggag tttcaggggc tgaagcccgc gacgaaaggc
     6541 aatccaacgg ccgagttaaa actcgatcct cctaaattcg agaaattttc aaattaggac
     6601 actgctggac ttccagtcgt ttctgcttgt cgctagagcg tttttgattt tagtgcacac
     6661 atatcccgaa gaagcgaagt agtgtgtgca ctctgtcgag gagaccgtgt tgaggacttg
     6721 accgattttc tcccagaggg agtcgacgct gcgcgcggcg gcttgccgaa gcagcgcttt
     6781 gagtttggca a
//
LOCUS       DASZMB010000120         5744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_5054550, whole genome shotgun sequence.
ACCESSION   DASZMB010000120 DASZMB010000000
VERSION     DASZMB010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5054550"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1558)
                     /locus_tag="VG272_05470"
     CDS             complement(134..1558)
                     /locus_tag="VG272_05470"
                     /inference="COORDINATES: protein motif:HMM:NF014697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HWF13165.1"
                     /translation="MGSISERNLTQGASEDSAGVFARLGMGLADWSQKWFPDAFVFAL
                     VGLIIVFVAGLFGGVGVGNLVKYFGDGFWGLIPFTMQMAMIIIGGFVVATSPPVHAFI
                     RKLAKIPRTPRTAVAFVVFFSMMTSLLSWGLGLIFAGILVREIVRNVRGIDYRAIGAA
                     AFVGNGSVWALGLSSSAALVMATPGSIPAALLKISGAIPLSLTIYTWQSATTAAILII
                     SSVTVGYFSTPVKSAKDAESFGVADGLAVKELEKRSTPAEWLEYAPALTILISILGLA
                     YIAQVLAVKGPMAALDLNTYNLLFLMLGLLLHWRPRSFVRAVNDSVPATAGVLIQFPF
                     YGGIFGIITYSALAAKLAHFFVSFSSTNSYPIVVSVYSAVLGMFVPSGGSKWIIEAPY
                     VLQAAKDLHVNLGWVVQVYNTSEALPNLINPFWMLPLLGLLKVKARDLIGYGMLYFMV
                     NFVLVLFFMWFFARTFPYVAPVIP"
     gene            complement(1662..3155)
                     /locus_tag="VG272_05475"
     CDS             complement(1662..3155)
                     /locus_tag="VG272_05475"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HWF13166.1"
                     /translation="MNRRDLFKTSLAASALALTGSRVDAAPAAPAEFPKAPGLTKSVA
                     EFIINTKYEDIPQDVIDLGKKSILDGFGLALAGSASVMGPIARKYAETFGPSEMRSSV
                     IGTGMKAHPRLASFVNGVSIHADDYDDTQLSAAKDRIYGLLTHPTVSVLPPAFALCEL
                     GHRSGKDFMVAYHMGVEVETKINEAISPRHYDEGFHSTGTCGSFGSTAACSRLRGLDV
                     KQTIYALGIGATEGGGFRDNFGSMTKPFQAGHAAENGTVAADLAALGWTAAVDILEAP
                     LGFFQAAGGGFDPNAIVGRFGKPWTLASPGVSIKPYPSGSLSHPAMGEMMRMIRENNI
                     KPGDVEKVDVGANHAMTTSLLHHVPMTGLQGKFSMEFCMSILLLDRKAGLVQFQDDVV
                     QRPDVQEMIKRIHFYIDPVAENAGLDKMTSIMKITLKDGKVITGHAEFAKGSPANPMT
                     YDEVADKFRGCAEFVKWPTAKAEAVIAAVKSLENISDMSKLTPSLTS"
     gene            complement(3184..4290)
                     /locus_tag="VG272_05480"
     CDS             complement(3184..4290)
                     /locus_tag="VG272_05480"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF13167.1"
                     /translation="MMRVARKFVAAVSISMVGIVALALAAHVSFAQQSTANAAKSAQA
                     MEPIPALTPFVEEHAHFDETDPVGSVRSALTEMGRENAAMILFQISPDTFDHPGHYDS
                     EVILAEVKKHPGKLAITGGGGSLNSIIMQSVATGDAGPAVQKKFKDRADELLREGVVG
                     FGEMTAEHFSGYTPYQYAPMDHPLYLMLADIAAAHHVPIDLHMEAVPQDMALPAGLKS
                     PPNAPRLHANIAAFERFLSHNPNAKVIWAHLGSDFTGFRTVDLDRRLLAAHPNLYMEI
                     KTDPVAPGLNYPLAADGKIKPEWLKLFTDFPDRFVIGSDQHYPEPKGEDRRWQEVILL
                     FNQLPANVRKKIGTENVASIYGNAVATYLRAAKN"
     gene            4559..4960
                     /gene="msrB"
                     /locus_tag="VG272_05485"
     CDS             4559..4960
                     /gene="msrB"
                     /locus_tag="VG272_05485"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HWF13168.1"
                     /translation="MSEKIRKSEAEWRKILTPNQYYVTRQKGTEPPGTGEYEDTEEPG
                     VYVCICCGQPLFTSETKFHSGSGWPSFYAPVDDKKVEVERDISHGMTREEVLCSKCDA
                     HLGHLFPDGPQPTGQRYCINSASIKFVEKDD"
     gene            5083..>5744
                     /locus_tag="VG272_05490"
     CDS             5083..>5744
                     /locus_tag="VG272_05490"
                     /inference="COORDINATES: protein motif:HMM:NF014336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HWF13169.1"
                     /translation="MQTRAWGCEGHQIIALLAEKHLTPHALEMAKKILADSPIDPSLS
                     RYCKEGGTDPLADASTWPDDMRGLRPETAPWHYVDIPLGTTHRDVEKYCDPKEGCVTR
                     AIANELAVLRSANSNPKDRADALRFLIHFVGDLHQPLHAATNNDEGGNCVPVAFFDLT
                     PQLRNPQAESYVPNLHGVWDTNILERATTGKNVDQVASDLNASFGKKIGDWQKGRANI
                     DA"
BASE COUNT         1347 a         1600 c         1541 g         1256 t
ORIGIN      
        1 cagctcgctg aaacgtgtca catatttagt gctcggagta ataagtcctg caaaatgagt
       61 gcggctttag ccgctgaagc ttcaattttg cagttctcac acagactcta aagcccgcgc
      121 gactcgaaaa ccatcaaggt attacaggag caacatacgg aaacgtccgc gcgaagaacc
      181 acatgaagaa cagcaccaga acgaaattga ccatgaagta gagcatccca tatccaatca
      241 agtcgcgcgc cttcaccttt aataatccca gaagcggcaa catccagaac ggattgatca
      301 gattcggcaa cgcctctgac gtgttgtaaa cctgcacgac ccacccgaga ttcacatgca
      361 aatccttggc agcctgcaag acatacggcg cttcaatgat ccatttgctg ccgccggaag
      421 ggacgaacat tcccagcacg gctgaataaa cggaaacaac gatgggatac gaattcgtcg
      481 aggaaaagct tacaaaaaaa tgtgccaact ttgcggcgag cgcggaatac gtgatgatcc
      541 cgaaaattcc gccataaaat ggaaactgaa tcagcacgcc tgcggtcgcg ggaaccgaat
      601 cgttgacggc gcgtacaaac gagcgcggcc gccagtgcag caagagcccc agcatcagga
      661 acagcaggtt gtacgtattc aggtccagcg cggccattgg gcccttcaca gcgagaactt
      721 gcgcgatgta agcgaggcca agaattgaaa tcaggatcgt gagcgcgggc gcatattcca
      781 gccattccgc gggtgtgctg cgcttctcaa gttccttcac tgcgaggccg tccgcgacac
      841 cgaaagattc agcatccttc gccgatttta ccggagtcga aaaatagccc acagtcacgg
      901 acgaaataat aaggatcgct gcggtggtgg cactttgcca ggtgtagatc gtcaggctca
      961 gcgggatggc gccgctgatc ttgagcagcg ctgcgggaat cgagcccggc gtggccatca
     1021 cgagagcagc ggatgacgaa aggcctagcg cccacacgct gccattgccc acaaaagcgg
     1081 cggcgccaat cgcgcgataa tcaatgccgc gcacgttgcg cacgatttcc ctgaccagga
     1141 tgcccgcgaa gatcagaccg agtccccagc ttagcaggga cgtcatcatg gaaaagaaca
     1201 ccacgaacgc gacggccgtg cgaggagtcc gcggaatctt tgccagcttc ctaatgaatg
     1261 catgcaccgg gggcgatgtg gctacgacaa atcccccgat aatgatcatg gccatctgca
     1321 tggtgaacgg aatgagcccc caaaatccgt caccaaaata tttgacgaga ttgccgaccc
     1381 cgactcctcc gaataatccg gccacgaaaa caatgatcaa gccgaccagc gcgaatacaa
     1441 atgcatccgg aaaccacttc tgggaccaat cagccagccc catgcccaaa cgcgcgaaca
     1501 cacctgcaga atcctcgctg gcaccctgcg tcaaatttcg ttctgaaatc gaccccatgc
     1561 gtcctttcgg attttgtagc gccggcgttt cgccgccggt tttgcgctca gtagagatca
     1621 gaaaaaccgg acggcggaac accggcgcta caacttcgcg cttagctggt cagggacggc
     1681 gtgagtttgc tcatgtcgga aatgttttcc aacgatttta cggctgcgat gacggcttcc
     1741 gcttttgctg tgggccattt tacaaattcg gcgcagcctc ggaatttgtc agccacttca
     1801 tcatacgtca tgggattggc agggctaccc ttggcgaatt cggcgtgccc ggtgattact
     1861 tttccgtcct tcaaagtgat tttcataatg ctcgtcattt tgtcgaggcc cgcgttttcc
     1921 gccactggat cgatatagaa gtggatgcgc ttgatcatct cttgcacatc gggacgctgc
     1981 acaacgtcat cctggaattg caccaggccc gccttgcgat caagcagaag aatactcatg
     2041 caaaattcca tgctgaattt tccttgcagt ccggtcatgg gcacgtgatg caggagcgat
     2101 gtggtcatgg cgtggttcgc gccgacatcc actttttcca cgtcgccggg cttgatgttg
     2161 ttctcgcgaa tcattcgcat catttcaccc atggctggat gtgaaagcga gccggatgga
     2221 taaggtttga tcgagacgcc gggagaagcc agcgtccatg gctttccgaa gcggccaacg
     2281 atcgcgttcg gatcgaaccc gccccctgcc gcttgaaaga atccgagagg cgcttcgaga
     2341 atatcaaccg cagcggtcca accgagcgcc gcgaggtccg ccgcgacggt tccgttttcc
     2401 gcagcgtgcc cggcctgaaa cggcttggtc atggaaccga aattgtcgcg gaaaccgccg
     2461 ccttcggtgg ctccaattcc caacgcgtaa attgtttgct tcacgtcgag accgcgcagg
     2521 cgcgagcaag cagccgtgct gccaaaactg ccgcaagttc cagtggaatg gaaaccttcg
     2581 tcatagtgtc gcggtgaaat ggcttcgtta atcttggtct ccacttccac gcccatgtga
     2641 tatgccacca tgaaatcctt gccagagcgg tggcccagtt cgcaaagagc aaacgcggga
     2701 ggaagcacgc tgaccgtcgg atgcgtcagc agtccgtaga tgcgatcttt tgcagcggag
     2761 agttgcgtgt catcgtaatc gtccgcgtga atggaaactc cattgacgaa agatgcgagt
     2821 cgcggatgag ccttcattcc cgtgccgatc acgctggagc gcatttcgct cggtccaaat
     2881 gtttccgcat atttccgcgc gatcggaccc atcaccgacg ccgagcccgc aagcgccagg
     2941 ccgaatccgt caagaatcga tttcttaccc aggtcgatta cgtcctgcgg aatatcctca
     3001 tatttcgtgt tgatgatgaa ttcagcaacg gatttggtga ggccgggagc tttcggaaat
     3061 tccgcgggtg cggcgggagc agcatccaca cggctgcccg taagggccag agccgatgcg
     3121 gccaaagaag ttttgaaaag atcacggcga ttcatgagtc aggccctcgt ttcgattctg
     3181 atttcaattt tttgcggcgc gcaaatacgt cgccacggca tttccataaa ttgaggctac
     3241 attttccgtc ccaattttct ttcgcacatt ggcaggcaat tgattgaata acaggatcac
     3301 ttcctgccag cgacgatctt cacctttcgg ttccggatag tgctgatcac tgccaatcac
     3361 gaagcggtcc gggaagtcgg tgaaaagctt cagccactcc ggcttgattt ttccatccgc
     3421 cgcaagcggg tagttaagtc cgggcgcaac gggatcggtt tttatttcca tatatagatt
     3481 tggatgcgcc gcgagcaaac ggcggtccag gtccacggtg cggaatccag taaagtccga
     3541 acccagatgc gcccagatta cttttgcgtt tggattgtgc gacagaaatc tttcaaacgc
     3601 agcaatattg gcatgcaagc gaggagcgtt cggtggggac ttcaagcctg cgggcagcgc
     3661 catatcctgc gggacggctt ccatatgcag atcaatgggc acatggtgcg ctgcggcaat
     3721 atcggccagc atcaggtaga gtgggtgatc catgggagca tactggtacg gcgtgtagcc
     3781 gctgaaatgt tccgccgtca tttcgccaaa gcccacgaca ccttcgcgca atagttcgtc
     3841 ggcacgatct ttgaattttt tctgtaccgc tggccccgca tcaccagtgg ccacggattg
     3901 catgattatg gaattcagcg agccgccgcc acctgtgatg gccagtttgc cgggatgctt
     3961 tttcacctcg gcaaggatca cttcggagtc atagtggcct gggtgatcga aggtgtccgg
     4021 cgaaatctga aaaaggatca tcgccgcatt ctcacgtccc atttcggtta gcgcggaacg
     4081 gaccgaccct acggggtctg tttcgtcaaa gtgggcatgc tcttccacaa atggggttaa
     4141 ggcgggaata ggttccatgg cttgcgccga tttggcggca tttgccgtgg attgttgtgc
     4201 gaacgagacg tgtgcagcga gcgccagcgc cacaatcccg accatcgaga ttgacacagc
     4261 cgccacaaat ttccgcgcca ctcgcatcat tgcgaacctt ccattcactg cacgttttta
     4321 taattggttg cgaccaagtc tactttttgt tcgtaagatt ctgtctgctg catgtgtccc
     4381 aacgcaagcg atttcttcca gtttggctcg gtccgtgatc gttcaccagt agcgcgggct
     4441 tcagcctgcg gcggctagcc tttgcaatga ctagaacccg caggctggta tcctcgctac
     4501 ataagaccaa gttctctaat ctcaggaatt cgggttaaga tattctcgcg aggcccctat
     4561 gagcgaaaaa attcgcaaga gcgaagctga atggcgcaag attcttacac caaaccaata
     4621 ttacgtgacg cgccaaaaag gcaccgagcc gcccggcacg ggcgagtacg aagacacgga
     4681 agaacccggc gtgtatgttt gcatttgctg cgggcagccg ctgttcacgt ccgaaacaaa
     4741 attccattcc ggttcaggat ggccaagttt ctacgcgcct gtcgatgaca agaaagtgga
     4801 agtcgagcgc gacataagcc acgggatgac gcgcgaagaa gtgttgtgta gcaagtgcga
     4861 cgcgcacctc ggccatctgt ttcccgacgg accgcaacct acgggtcagc gctattgcat
     4921 caattccgcc tctattaaat ttgtcgagaa agatgactga tgccgcgtgc cgcggtgtct
     4981 tccattttgt ttttctctat atcctgtaaa tcctttcggc tacgtttcgc ccgtgccgga
     5041 atttttgcgt ctgtattatt tttaggcatt gtgagcttgc ctttgcagac ccgcgcctgg
     5101 ggttgcgaag gacaccaaat aattgcgctg ctcgccgaaa agcacctcac tccgcacgct
     5161 ctggaaatgg ccaaaaaaat tctggccgat agccccatcg atccaagcct cagccgctat
     5221 tgcaaggaag gcggcaccga tccgctggcc gatgcctcga catggcccga tgacatgcgc
     5281 ggcttgcgcc cggaaaccgc cccgtggcat tacgtcgata ttccgctggg tacaacccat
     5341 cgcgacgtcg agaaatactg cgatccaaaa gaaggctgcg tgacgcgcgc catcgccaat
     5401 gagttggctg tgttgcgttc cgccaattcc aatccgaagg atcgggcgga cgctttgcgc
     5461 tttctgattc acttcgttgg cgatttgcac cagcccttgc atgcggccac gaataacgat
     5521 gaaggcggca actgcgttcc ggtggcgttt ttcgacctca ctccccagct aagaaatccg
     5581 caggcggaaa gttatgtgcc gaacctgcat ggcgtgtggg atacaaatat tctggaaaga
     5641 gcgaccaccg gtaaaaacgt agatcaagtg gcatcagacc tgaacgcgtc tttcggtaag
     5701 aaaatcggtg actggcaaaa aggtcgtgcg aatatcgatg cgtg
//
LOCUS       DASZMB010000121         5580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_107990, whole genome shotgun sequence.
ACCESSION   DASZMB010000121 DASZMB010000000
VERSION     DASZMB010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107990"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1182
                     /locus_tag="VG272_05495"
     CDS             97..1182
                     /locus_tag="VG272_05495"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HWF13170.1"
                     /translation="MSPIRNISADSKPAPFSLGYRMPAEWEPHTATWLAWPHEKTDWP
                     GKFAPIPWVYADIVRHLSQVERVRILVKDVAMERAALHTLKSAGAYLSAVDFFQVPTN
                     RGWIRDFGPIFVRNAKGETAATHWHFNAWAKYDDWKNDDAANAKLARKLKWNMWSPTY
                     RGRRLVLEGGSIEVNGQGTMLTTEECLLSPVQARNPGISAKEIAAIFRDYLGVKNLLW
                     LKNGIAGDDTHGHVDDLARFADPTTVITVVENDQSDANYQPLQENLALLREMKDQDGR
                     PLRIETLPMPDPVYFDEQRLPASYANFYIANRLVLVPTFNDPHDATALIKIAALFPGR
                     KIVGISCLDLVLGLGTLHCMTQQEPDP"
     gene            1417..1692
                     /locus_tag="VG272_05500"
     CDS             1417..1692
                     /locus_tag="VG272_05500"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HWF13171.1"
                     /translation="MHIYSLYIPQSVITWIIVGLIAGWIAGTVSRGRGFGCLADIILG
                     MVGAILGGWIFLKLGILGGGLLYSIAAATVGAVLLVAIASIFSGGNK"
     gene            complement(1723..2235)
                     /locus_tag="VG272_05505"
     CDS             complement(1723..2235)
                     /locus_tag="VG272_05505"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HWF13172.1"
                     /translation="MGEKGASKQEVVVFQGKRIRYHSGLSFDEVLGKLRSRMGKIKLA
                     DVLDPSSSRADFEKKVKEHEGESGFMLFVEMDHGSWMRKFGVNRKVVRWIFGNPLVAI
                     TMLRHDYTAGLFVPPDLLLAEKDGGVGCTITYVLPSSLISVEENAPLRAAALALDAKV
                     KALVSSAATP"
     gene            2556..4220
                     /locus_tag="VG272_05510"
     CDS             2556..4220
                     /locus_tag="VG272_05510"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HWF13173.1"
                     /translation="MDKPSLLAIPLGGLGEFGMNMLVLRSGDDMILIDAGMMFPEAEL
                     LGVDIVIPDITYLKQNKQFLRAILLTHGHEDHIGALPYILRDLQVPIYGTRFTLALVH
                     KRLEEHGILDKTKLCEVIPGRMLEVGPFEVEFISVTHSTIDCVALAIRTPVGVVIHTG
                     DFKIDQTPTDGQPFDMHAFARYGAEGVLALFSDSTNVERPGYTPSERTVRPRIEELTR
                     AATRKVIVSCFASSIPRIQQIIDIAAAANRKISFIGRSMVDNVEIAHGMNRLRIPDGL
                     VVRPQDISSFPPEKMIVLVSGSQAEPMSSLSRVAVDNHRLLTLDERDTVILSSRIIPG
                     NEKAIFRMLDHMFRKRALVYYDNDGGTIHVSGHASQEEQKLLLNLVRPRYFIPIHGEY
                     RQLFRHAGLAHVVGAVSEEILLIEDGQPIEFTEERAYRREPVTAGRVCVDSGSLEEIE
                     EVVIRDRKHLSEDGVVVPIIAIDKHTGQIESQPEIVTRGFMPSDNVPELINGAREVLL
                     RTVEQSNDEEKTDWSVIKEKIRVDLRRYLKKQTSKRPLILPVILEL"
     gene            4293..4721
                     /locus_tag="VG272_05515"
     CDS             4293..4721
                     /locus_tag="VG272_05515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HWF13174.1"
                     /translation="MDQSCFPKTIAYGRREMKAYLQTRGSHCIVAEISGAQTHEGSAE
                     TIAGFILTERDAEFAHIITLDVLETFRRKSLGSLLLQAAEQEATSHGAGSMYLETAMT
                     NKAAIALWKKHGYVETGVIVNYYGRGQHAFEMRKRLDRKS"
     gene            4737..>5580
                     /locus_tag="VG272_05520"
     CDS             4737..>5580
                     /locus_tag="VG272_05520"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HWF13175.1"
                     /translation="MTLLHVVILAIVQGLAELLPVSSSAHVVVSEKLMGLDPTSPEMT
                     LLLVMLHTGTMFAVIVYFWKQWAKTYFQSAEAFKQIVIMLVVATALTGVIGEGLIKAI
                     EKFAFPGVEHAEVEQLFGRLDLIAPALAAVGIFILIAGIYEKRHPFVGNKDTGGLDMK
                     RASIIGIVQGLCLPFRGFSRSGATISAGLLADIQKTRAEAFSFALAVALTPAADGREV
                     LRLMKANHIAGGLNLASAMVPSLIGMVCAFAAGLLALKWLSKWLESDRWHLFGIYCLA
                     AAIAV"
BASE COUNT         1286 a         1628 c         1421 g         1245 t
ORIGIN      
        1 tgggtcgcgg cttcagccgc gacattccgt tcgcacgaat aagtgggctt tagcccctga
       61 ccggcatagt gtagcgctcc cgctgggatt agtgagatga gtcctatcag aaacatcagt
      121 gccgattcaa agcccgctcc cttctctctc gggtaccgca tgcccgccga gtgggagccg
      181 cacaccgcta catggctcgc atggccacac gaaaaaaccg actggcccgg aaaatttgcg
      241 cccattccct gggtgtatgc ggacatcgtc cgccacctct cgcaggtaga gcgcgtgcgc
      301 atcctcgtaa aagatgtggc aatggagcgc gcagcactgc acacgctgaa atccgcaggc
      361 gcctatctct cggccgtcga tttttttcag gtgcccacaa accgtggatg gattcgcgat
      421 ttcggcccca tcttcgtgcg caatgcgaaa ggcgaaaccg cagccactca ctggcacttc
      481 aatgcatggg cgaaatacga cgactggaaa aatgacgatg ctgcaaacgc aaaacttgcg
      541 cgcaaattaa aatggaacat gtggtcgcca acgtatcgcg gacgccgcct agtccttgaa
      601 gggggcagca ttgaagtaaa cggccaaggg acgatgctga ccacggaaga atgcctgctc
      661 agcccggtcc aggcacgcaa tcccggcatc tcggccaaag aaatagcagc aattttccgc
      721 gactacctcg gcgtaaaaaa tcttctgtgg ctgaaaaatg gaatcgccgg cgacgatacg
      781 cacggccacg tggacgatct cgcgcgcttc gccgatccaa ccaccgtcat cactgtggtg
      841 gaaaacgatc aatcggacgc gaattatcag ccgctgcagg aaaatctcgc cctactgcgc
      901 gaaatgaagg atcaggatgg tcgcccgctg cgcatcgaaa ctttgccgat gccggacccc
      961 gtgtatttcg acgagcagcg cctccctgcc agctacgcca atttttacat cgcaaaccga
     1021 ctcgtcctcg tcccgacatt caacgatccg catgacgcga ctgcgctgat aaaaatcgca
     1081 gccttatttc ccggccgcaa aattgtagga atatcatgcc tggatctcgt gttaggtttg
     1141 ggcacgctgc attgcatgac gcagcaggaa ccggaccctt aagtggcata ggcttcagag
     1201 tctgtgtgag aactgcagaa tctttgtggg tcgtggcttt agccacgaca taagttctgc
     1261 aaaatgagtg cggctttagc cgctgaagct tcaattttgc agttctcaca cagactcttc
     1321 agcctgtgag ggttaggcct tgcgagaact aactgccaca ggctaaagcc tatgccacat
     1381 aaaccgatcg cttttactta aatttcggag ttcgaaatgc acatatactc gctctacatc
     1441 ccgcaatccg taatcacctg gatcatcgtc ggcctcatcg ccggctggat cgccggaacc
     1501 gtttcgcgcg ggcgcggatt cggatgcctc gcagatatta ttttgggaat ggtgggagca
     1561 atcctcggcg gctggatttt tctaaagctc ggcattctcg gcggtggcct tctgtacagc
     1621 atcgcggcag ctactgtggg cgcggtctta ctcgtagcga tcgcaagtat attttcaggc
     1681 ggcaacaaat aagatttcaa aaaattacat gcaatcacgc aattacggcg tggcggccga
     1741 agaaacgagt gctttcacct tggcgtccag cgccagagcc gctgcccgaa gcggcgcatt
     1801 ttcctccaca gaaatcaggg aagatggaag aacgtaagtg atcgtgcagc ccacgccgcc
     1861 gtctttttcc gcaagcaata aatccggcgg cacaaagaga cccgcggtat agtcatgtcg
     1921 caacatggtg atggccacaa gtgggttgcc gaaaatccag cgcactacct tgcggttcac
     1981 tccaaacttc cgcatccagc tcccatgatc catttctaca aacagcatga aaccgctttc
     2041 gccctcgtgt tctttcacct tcttttcaaa atccgcgcga ctgcttgacg gatccaaaac
     2101 atcggcaagc tttatttttc ccatgcgaga ccgcagcttg cccagaactt cgtcaaaact
     2161 taacccgctg tggtagcgga tgcgcttccc ctgaaaaaca accacttcct gcttacttgc
     2221 acccttttcg cccatttctc ttttctcccg taccgcaccg tccatagcta gggtttcctg
     2281 gaagccatct ccaaagacca tcttcacaga tcatctctag cggccatctc atacttaatc
     2341 tttgtgggtc gcggattcag tcgcgacatt cagagactca aaaaacaaag gctttagcac
     2401 tgttgcactg tcgagtttgg aagacatgcc atttatgaca tcgctgttag tcgaatagat
     2461 aaactttgct ggcgatcttc gttccgcgtc gcggcaaatt cgcgtgcgcc tgtgtgataa
     2521 gatgctgagt cttaattata aggacgcgaa aaacattgga taaaccttct ctcttagcaa
     2581 ttcccctggg cggactgggc gagttcggca tgaatatgct cgttctccgt tcgggcgatg
     2641 acatgatttt aatcgacgcg ggcatgatgt tccccgaagc cgaactgctc ggcgtggaca
     2701 tcgtcattcc cgacattacg tacctcaagc agaacaagca gtttcttcgc gccattttgc
     2761 tgacgcacgg tcacgaagac cacatcggcg cgctgccata tatcctgcgc gacttgcaag
     2821 tacccattta tggcacgcgc ttcacgctgg cactcgtgca caagcgcctt gaagaacacg
     2881 gcattctcga caagaccaag ctttgcgaag tgatcccagg gcgcatgctg gaagtcggtc
     2941 cgtttgaagt cgagttcatc agcgtcacgc atagcacgat cgattgcgtc gcgctggcga
     3001 tccgcactcc ggtcggcgtt gtcatccaca cgggcgattt taaaattgat caaactccca
     3061 ccgatggcca gcctttcgac atgcatgctt tcgcgcgcta tggcgcggag ggtgtccttg
     3121 ctctgttcag cgatagcacc aacgtcgagc gccccggtta cacgccctcg gagcgcactg
     3181 ttcgcccgcg aatcgaggag ctcacgcgcg cggcgacacg aaaagtcatc gtctcttgct
     3241 tcgcctcatc gattccacgc attcagcaga tcattgacat cgccgccgcc gcaaatcgta
     3301 aaatttcttt catcggccgc agcatggttg acaacgtgga aatcgcgcac ggcatgaacc
     3361 gcctgcgcat tcccgatggg ctcgtcgtgc gcccccagga catcagttca tttccgcctg
     3421 aaaaaatgat cgtcctcgtc agcggcagcc aggcagaacc gatgtcctcg ctctctcgtg
     3481 tggccgttga caatcatcgc cttctcacgt tggatgagcg cgacacagta attctttcgt
     3541 cgcgcatcat cccgggcaac gaaaaagcca tcttccgcat gctggatcac atgttccgca
     3601 aacgtgccct cgtttactat gacaacgacg gcggcacgat tcacgtttcc ggtcacgcca
     3661 gccaggaaga gcagaaactg cttctgaatc tcgtgcgccc gcgctacttc attccgatac
     3721 acggagaata ccgacaactc ttccgccatg ccgggctcgc ccacgtggtc ggcgcagtct
     3781 ccgaagaaat tctgctgatc gaagacggcc agccaattga attcacggaa gagcgcgcgt
     3841 atcgccgcga gcctgtgacc gccggccgcg tttgcgtcga ttcaggctcg ctcgaggaaa
     3901 tcgaggaagt ggtaatccgc gaccgcaaac atttatcgga agacggcgtc gtcgtcccca
     3961 tcatcgcgat cgacaagcac acaggacaaa tcgagtcgca gcctgaaatt gtgacgcgcg
     4021 gctttatgcc ctccgacaac gttccggaat tgattaatgg cgcgcgtgaa gtccttctcc
     4081 gcaccgttga gcaatccaat gacgaagaaa aaaccgactg gagcgtcatc aaggaaaaaa
     4141 ttcgcgtgga cctgcggcgt tatttgaaga agcaaacttc caagcgcccg ctgattcttc
     4201 ccgttattct cgagttgtga gccaaagctg tgtcctttcc cggtttacac ctgcgaccat
     4261 accagccatc cgatttcgac accctcgtag cgatggatca atcgtgcttt ccaaaaacta
     4321 tcgcctacgg acgtcgtgag atgaaagcct acctgcagac aaggggatct cactgtattg
     4381 tcgcggaaat ttccggagcg caaactcacg aggggagcgc ggagaccatt gccggcttca
     4441 ttctcacgga acgcgatgct gaatttgccc atatcattac actggatgtt ctcgaaacct
     4501 ttcgccgcaa gtcgcttggt tccctgcttc tacaagctgc agagcaggaa gcaacctccc
     4561 atggcgccgg tagcatgtat ctggaaacgg caatgacgaa caaagctgca attgcattgt
     4621 ggaagaagca tggctatgtt gagactggcg taattgtaaa ttactatgga cgcggccagc
     4681 acgcttttga aatgcgaaaa cgattagatc ggaaatccta aatctaaggt cccctcatga
     4741 cacttctgca tgtagtgatc cttgccatcg tgcaaggcct ggcggaactc ttgccggtat
     4801 cgagttccgc acacgtcgtc gtctcggaaa agctcatggg cctggatcca acgtctcctg
     4861 aaatgacgct gctgctcgtc atgctgcaca ccggcaccat gttcgcggtg atcgtctact
     4921 tttggaagca gtgggcaaaa acatatttcc aaagcgcgga agcgttcaag cagatcgtga
     4981 tcatgctcgt cgtcgccaca gctctcacag gagtgatcgg cgaaggcctg atcaaagcga
     5041 ttgaaaaatt tgcgtttcct ggcgtggagc acgcggaagt cgagcagcta tttgggcggc
     5101 tggatttgat cgcccccgca cttgccgctg tcggcatatt cattttgatt gcaggaattt
     5161 acgaaaagcg ccatccattc gtaggcaata aagacactgg cggcctggat atgaaacggg
     5221 cttccatcat cggaatcgtg caagggttgt gcctgccctt ccgcggcttt tcgcgctccg
     5281 gtgcaaccat ttccgccggc ctactcgcag atatacagaa aacgcgtgcc gaagccttca
     5341 gcttcgccct agccgtggcg ctcactcccg cagccgacgg ccgcgaagtc cttcgcctta
     5401 tgaaagccaa ccacatcgcg ggtggcctga atctggcgtc agccatggtc cccagcctga
     5461 tcggcatggt gtgcgcattc gccgccggcc tgttggccct caaatggcta agcaagtggc
     5521 tggaaagcga ccgctggcac ctcttcggaa tttattgcct cgcagcggcc atagcggtcg
//
LOCUS       DASZMB010000122        20799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1503315, whole genome shotgun sequence.
ACCESSION   DASZMB010000122 DASZMB010000000
VERSION     DASZMB010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 20799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20799
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1503315"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..642)
                     /gene="mscL"
                     /locus_tag="VG272_05525"
     CDS             complement(205..642)
                     /gene="mscL"
                     /locus_tag="VG272_05525"
                     /inference="COORDINATES: protein motif:HMM:NF001843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HWF13176.1"
                     /translation="MLKEFKEFAMRGNVLDMAVGIIIGAAFGQIVTSFVQDVMMPPIG
                     RLLGHVDFSNLFINLTETHYPTISAAKAAGAATLNYGLFLNTIINFLIVAFAVFLLVR
                     QVNRLAPPPAPAPPVTRDCPFCLGAVPIKATKCMHCTSEIRPA"
     gene            complement(682..1062)
                     /locus_tag="VG272_05530"
     CDS             complement(682..1062)
                     /locus_tag="VG272_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13177.1"
                     /translation="MLTPANLFRMMTEFIFILLGGFLVWLGLSNRFLNPVNFDPRSPE
                     WLGLGAVLIYWGVRTWLKTSRAARTPERTVARVGGASLVIVGIAMLTFVFLTFRWVGL
                     ALAAVGGVLALRGLLAAVLSLRTD"
     gene            complement(1080..1604)
                     /locus_tag="VG272_05535"
     CDS             complement(1080..1604)
                     /locus_tag="VG272_05535"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWF13178.1"
                     /translation="MSRAPGTYAIFDTTQGTVVVKLFEKEAPQTVGNFVGLAEGTKEF
                     KDPKTGQKVKRPFYDGLIFHRVIPQFMIQGGCPLGTGTGDPGYRFGDEFNSSLKHDKP
                     GKLSMANAGPGTNGSQFFITVAPTPWLDNKHTIFGEVVEGMDIATKISELPRGAGDKP
                     KTPVELKSVKIERS"
     gene            1812..2729
                     /gene="trxB"
                     /locus_tag="VG272_05540"
     CDS             1812..2729
                     /gene="trxB"
                     /locus_tag="VG272_05540"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HWF13179.1"
                     /translation="MIIIGSGCAGMTAAIYAGRANLKPVVIDGREPGGQLALTTMVEN
                     FPGFPDGILGPDLIENMRKQAQRFGAEFKSGAITDVDLSKRPFKITAGKETYEAQALI
                     IAAGATARLMGLESERKLIGYGVSTCATCDGYFFRGKDIAVVGGGDSAMEEANFLSRF
                     ASKVYLVHRRNEFRASKIMVDRARANEKIQFVTPVIVEDISDVTKGGVENMKLRNAET
                     SELSTLAVEGVFIAIGHDPNTKVFRGKLDMDENGYLTTHHGSQTNVPGVFAAGDVQDH
                     HYKQAVTAAGSGCMAAIDAEKFLEAHKGA"
     gene            2769..3695
                     /locus_tag="VG272_05545"
     CDS             2769..3695
                     /locus_tag="VG272_05545"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HWF13180.1"
                     /translation="MLRALLLELAKSSRLRRWITSHGVTRRMAHRFVPGEELGAAIEA
                     VRACNQAGMTASLDHLGENVVTREDAERARASYTEALERVAADDVDSNVSLKLTHLGL
                     DLGGEFCAEQLRIILRRAQELGNFVRVDMEGSAYTDRTLQMVKQARVETAAVGTVIQA
                     YLYRSEEDIRELLAIGCRIRLVKGAYKEPSQIAFPRKKDVDANFIKLMKILLLSGIYH
                     ALATHDPKMIDAATRFAAEQNISKDKFEFQMLYGIRTDLQSQLVKQGYRVRVYIPFGR
                     DWFPYFMRRLAERPANLLFFAKNFFRGAAPAA"
     gene            3764..4768
                     /locus_tag="VG272_05550"
     CDS             3764..4768
                     /locus_tag="VG272_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HWF13181.1"
                     /translation="MIQNKFMRAMVLDQPGKPLTLRNLPVSQPGSHQVLLQVLTCAVC
                     RTDLHVVDGELPDPKLPLIIGHEIVARVVGLGENVSLLSVGQRVGVPWLGWTCGKCFY
                     CCTGSENLCVNARFTGYTLDGGYAECTVADERYCVPIPESYSDAEAAPLLCAGLIGYR
                     SLVRAGDAKRLGIYGFGAAAHIIAQVAVHQGRSVYAFTRAGDKEAQSFAKKLGAIWAG
                     ASEERPPDELDAAIIFAPVGALVPAALAAVRKGGKVVCGGIHMSDIPSFSYDLLWGER
                     SVSSVANLTRRDAAEFMQIAPKVPVRTTTENFKLEQANEALKQLRNGKVHGAAVLKCS
                     "
     gene            4943..5209
                     /locus_tag="VG272_05555"
     CDS             4943..5209
                     /locus_tag="VG272_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HWF13182.1"
                     /translation="MAYVIAEPCVGTKDTACVDVCPVDCIHPRKDEPKFESEPQLYIS
                     PVECIDCGACVPVCPVTAIFALEDLPEKWANFTPINADWYTNQG"
     gene            5491..6612
                     /locus_tag="VG272_05560"
     CDS             5491..6612
                     /locus_tag="VG272_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HWF13183.1"
                     /translation="MEVAEIPANRQEPRTKIKNNIVPAVATIAGTATAVPPHVLSRDL
                     VKNGIGPVFNLEGSKLDAIREIIDNSAIDQRYSVFPSDYLIEPRPLAQINAEYQEKSV
                     ELGLQVAEHALAQAGMVPTDVDLLITVSCTGVMIPSLDAHIATIMGFRPDVRRLPITE
                     LGCAAGAAGLARTWEYLTAFPDRTAMLIAVELPTLTFQRKDASQANLISAALFGDGAA
                     GVVVTGREAPGPRILASECFLFPDSLGAMGFDLRDTGFHIVLSKDVPEIIRARIKGLA
                     QGFLARHGLRQEDISAFLLHPGGQKLLSYMQEELGLKRSDTEVSWDILRRYGNLSSAS
                     VLFILNEWLVQKEMPSGSYGLLMAFGPGFTAEMLLLQWP"
     gene            6603..7220
                     /locus_tag="VG272_05565"
     CDS             6603..7220
                     /locus_tag="VG272_05565"
                     /inference="COORDINATES: protein motif:HMM:NF016062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HWF13184.1"
                     /translation="MAMTFAYISLLVIVGIGRLAELVISRRNQRRLEKQGVRKIHEPL
                     FRFLVLLHGSILVCAGLEVLFLHRPLIPSLAISMGIVFILSNVLRWWVIWSMAGHWNV
                     EIMDSARVGVVSSGPYRWVKHPNYVGVILEVFSLPMMHTAWITAILGTLGYMEIIRQR
                     LKLENGALMASPEYRLIMGNKPSFFPRIFSQGPKDPAPERTRAGL"
     gene            complement(7288..7656)
                     /locus_tag="VG272_05570"
     CDS             complement(7288..7656)
                     /locus_tag="VG272_05570"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HWF13185.1"
                     /translation="MDAHEKDRLYKEGLEAFNSARFYDAHEHWEEVWLKTPNPEKIFL
                     QGLIQVAAAFHHHSRANLLGTRNLLREGLLKLDRFPEIYGDLEIETLRVTVREWLADF
                     DAGEIPASRKIPRIDRSRKK"
     gene            7839..10010
                     /locus_tag="VG272_05575"
     CDS             7839..10010
                     /locus_tag="VG272_05575"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HWF13186.1"
                     /translation="MSKFNLVLLIHAHQPVGNFDDVMERTYQQSYLPFVECVARHPRV
                     RMGLHYSGSLIEWMAEMHPEYIQRVGALVARGQVEVVGGGFYEPILVAIPYEDRIDQI
                     RRQSDFVEKLFGKRPQGAWLAERVWEPQLPTALAEAGVDYTLVDDSHFLTAGREIPEL
                     FGYYVADDCGKMVKVIPGLQELRYLLPFGTVEDSIAFLRRSATDHPGSMASMGDDMEK
                     FGAWPHTYEHCFRDGWLDRFFSAIEANFEWLEMVPPGEALASHAPLGRVDLPTASYTE
                     MMEWVLPTSVRQKFHSLLEEFSGRPDVRLFLRGGFWRGFFSKYAEANLLHKKMLRVSS
                     KLRGDGKKSSRRGTKGAVLRSRAITHLLRAQCNDGYWHGIFGGLYAPHLRTALWRELV
                     RAETIADGLRHGAKPYQTMTRLDFDADGREEIEITAPEFAALINPSRGGTLEVLDFRP
                     SAVTLINSLQRRVEAYHSRLQNMSQSAGKVASIHDQTLAKEPGLEKYLKYDRWPRNTF
                     RLLLFAPGKTHADFEALRLEESAPFAGGDYHVDQASAEEVTLTKEALLRQVNAGADLK
                     SILAATKILQFEKQEHGFDVRCRLELALKDAEYMAEGADDAVNILVGLEIVINLLAPN
                     VPDRYIEFAGVRKPLEWSGVVEEKKLRIADEWQNVAVEVEAPGACHFWISPIQTVSES
                     EEGFERVYQGSQILGLWNVKLDPSKPWSAETVLHVSKAHNP"
     gene            complement(10024..10992)
                     /gene="egtD"
                     /locus_tag="VG272_05580"
     CDS             complement(10024..10992)
                     /gene="egtD"
                     /locus_tag="VG272_05580"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HWF13187.1"
                     /translation="MATAVAAQHSRSELAREVRAGLCRGGQKELPSKYLYDEIGSALF
                     EVITLLPEYGLTRADERILHDHAAEIVSRLTPQVLVAELGSGSGKKTRWILEALALRQ
                     RTTYCPIEISPTALARCESELGQIECISIVGFERPYLDGLLAASAHRQENDHLLVLFL
                     GSTIGNFDRDAGERFLSEVRRILFPGDALLLGTDLIKPMPQLFAAYDDALGVTAAFNM
                     NLLARINRELDANFNLTEFRHEARYSEGHQRVEMHLRSLRDQTVKIPGAGCSVSFLKD
                     ETIWTESSHKYGAEDIPAMAERNGFRCDSQWIDKQWPFAENLLFAV"
     gene            complement(11025..12356)
                     /locus_tag="VG272_05585"
     CDS             complement(11025..12356)
                     /locus_tag="VG272_05585"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HWF13188.1"
                     /translation="MTSTAAEQSSRADRLFARLQEARARSEELFGIVHPGALYDRPVA
                     ERHRIIFYLGHLEAFDWNLLRETLGLTSFGPDFDRLFAFGIDPVEGGLPMDQPGDWPT
                     VERVREYNQRLRDLLNAAIRDAGVKDPEMGRLDLGRLLDVAIEHRLMHSETLCYMLHQ
                     LPGNRKLAQHTPPAPFAPAHLQRSITIPEGTATLGLSRKEGGAFGWDNEFEENRVQVP
                     AFRIDAFNVTNRDFLQFLRDGGYERRDLWTDAGWSWISSQGHRAPAFWLPKGDTWGYR
                     AMFAEIPLPLDWPVYVSHDEASAYARWVGKELPSEAEWHRAALDTPEGTERAYPWGSD
                     APSTQHGNFDFQRWDPVSVGSYPAGNSAFGVADLVGNGWEWTRTPFEPFSGFQPFSFY
                     PGYSADFFDGKHFVMKGGSPRTAACMLRRSFRNWFQPHYPYVYAKFRCVER"
     gene            12515..13300
                     /locus_tag="VG272_05590"
     CDS             12515..13300
                     /locus_tag="VG272_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWF13189.1"
                     /translation="MTSPTDSLQDGYALTFQNASYRLPSGSTLISSLDLHVARGETLI
                     LLGRSGSGKTTTLKLMNRLLDATEGEILIAGKPIRTLDPIELRRGIGYVIQDAGLFPH
                     WTVERNVGLVPRLEGWPEEKIEARVQEMLSLVGLPPEEFAERRPSGLSGGQKQRVGVA
                     RALAADPPILLMDEPFGALDPITRADLQNEFRTLAARLQKTIVFVTHDVREALYLGTR
                     IAVLDQGRLVGVYTPTEFLKAKEPEIHAFAASLHSGAAGEGAE"
     gene            13297..13929
                     /locus_tag="VG272_05595"
     CDS             13297..13929
                     /locus_tag="VG272_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF13190.1"
                     /translation="MKWYEFLLHNRAEVFQRIIEHLGLVAASMAIALAIGLPVGIALV
                     RRAKLQRWVIGGANVLQTIPSLALFGFLIPVPLIGGVGASTAIVALFLYALLPILRNT
                     CIGIIEVDPAVVEAARGMGMTPRQVLWQVQLPLAAPVLLAGIRVATAMCIGIATVAAA
                     IGAGGLGVFIFRGLAMVNNQVILAGAIPAAGIAIAVDSGLGYFQKSVDKR"
     gene            13926..14858
                     /locus_tag="VG272_05600"
     CDS             13926..14858
                     /locus_tag="VG272_05600"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HWF13191.1"
                     /translation="MNRSPGWTTLKRRVPRFATCMLVLGVLASLFAASCKKQSGGETI
                     VVGSKNFTEQIILAELFAQQIEAHSALRVERKLNLGGTFICHDALVSGKIDLYPEYTG
                     TALIAVLKEPPQNNPSEVFRRVKEEYQSRFNVEEMPPLGFNNTFAMVIRGDDAEKLHL
                     RTVSDIAPYAPKWRAGFGYEFMERPDGYQGWIEAYGIHFANTPRILDLGLLYRALADK
                     KVDLVAGNSTDGVIASLHMVVLEDDRHYFPPYEAVPLVRRATLERHPEIRDAIGALAG
                     KISVDEMRTMNYAVDGEQRDPADVVRAFRKSKGL"
     gene            14998..15294
                     /locus_tag="VG272_05605"
     CDS             14998..15294
                     /locus_tag="VG272_05605"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWF13192.1"
                     /translation="MKENRIEPRFNMNLPIVAWWEDEAGKQRFSHGKLEDISNAGLRF
                     KSREPIPVGTKLLLRTPMGEYVGAVVRSSQDGEEFNLGIKKISERKPSGTHVKL"
     gene            15421..16494
                     /locus_tag="VG272_05610"
     CDS             15421..16494
                     /locus_tag="VG272_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWF13193.1"
                     /translation="MELVPSFDMTFDNRKLTNFAKALRGRRIGVAGDFMLDRYVWGSA
                     TRLSPEAPVPVVDFKDQTQVLGGAGNVARNLAALGAKVLPFGVVGEDEDGLAIRELFE
                     EDGTATKGVVADASRITTVKTRIVAKNQQIVRVDRERREPIHGALEDRLGRAILKALP
                     RLDALVISDYDKGVITDMLADRVLTECQRRGVPALVKPKESRLLFYRGASMIVCNAKE
                     AHFFISRKFEDDESVEQGGRALLAHFGCAAAVITRGREGLNVFEKDEKKSFHVSATSS
                     EISYDRVGKAGVERSAHGRQVFDVTGAGDTVLSVLALAIAGRIPIREAAVLANAGAGV
                     VVGKLGTSTITPEELFAAVRELR"
     gene            complement(16628..17080)
                     /locus_tag="VG272_05615"
     CDS             complement(16628..17080)
                     /locus_tag="VG272_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13194.1"
                     /translation="MGLLDAGAEEPKSKALRYVISGVVLVLLIAGAIWFLLRYSTEKH
                     TATRFMNAIVAGDTQQAYQIWHPHPGFSYPDFLSFWGPNGYYSPIKSYQIETAEVPPK
                     GGTGVVVVIELSEYGTFPKAEETIKSARNREVQLWIERSDQSMSFPPP"
     gene            complement(17103..18254)
                     /locus_tag="VG272_05620"
     CDS             complement(17103..18254)
                     /locus_tag="VG272_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF13195.1"
                     /translation="MKFQRISAIIERDMRKFFRSPALMMSSMIFPLMQLIVLGYAFGG
                     KIKGIKVAVVDEDRTVYSRDLRERFNAIVAGPETMKVENYNSVSDAIVDLRAGFVRAV
                     VYIPVDYSRRVNQGNRPQVAFIEDNTDNFVTSEILTRMQDMVKDINQGLNPFQPGQRL
                     TVPLPRLNPLVNLQVVELYPYIEYIKYLLSGSIAMSVFIVAMIGGGITFIDDKSRGLH
                     EGYLVTPITKTELILGLIFSGAIKGLMAGTTITIIGGLIAGIDHLWDPVRLLFLMSVL
                     VITSIAMISFMFLLMVRVSDPLVPRAIFGVLNTLLYFPSGAIYPVEGFPVWLRFISYI
                     DPFTYAVHALKALLLKNTGFSGIYSDVLILTGFSIVLVALSVALFKRQI"
     gene            complement(18272..19270)
                     /locus_tag="VG272_05625"
     CDS             complement(18272..19270)
                     /locus_tag="VG272_05625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWF13196.1"
                     /translation="MNNIETNAIEVTKLRKQFGDFVAVKEVSFAVHTGEIFGLLGPNG
                     AGKTTLIRMMTTLTPPTSGSANVGGHDVVRDANGVRHAIGVIPQALTSDPELTARENM
                     NIHAKLYGLATSARERLVPQLLESVGLTEFSDKLVGSFSGGMRRRLEIARGLVHSPKI
                     MFLDEPTTGLDPVSRTAVWEMITKLKESAGLTILLTTHYMDEADKLCDRIAIVDHGQL
                     VALDTPIKLKDSVPAVDIVEAEFENPPADWTATLEKLKGVASVAVNDDFTHISTTNGP
                     LTVRELMDTARERGVNVRRVSVQGTTLDDVFLYYTGRQLRDAASETMKRDMSHLYK"
     gene            complement(19486..20562)
                     /locus_tag="VG272_05630"
     CDS             complement(19486..20562)
                     /locus_tag="VG272_05630"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWF13197.1"
                     /translation="MKFSKFFVFVGVLFAVSVVVYLTTTPRGTSIPLTGIVTGNDVIV
                     SSQIAGRMIKLNVDEGSEVHKGDLIAEIDPAEWVTARDSAEANIHMLENKVISASGTR
                     SMTDDQTSASIRQAEATLTSVRSQLAQAQANLWRDQTTYKRTQSLFDAGVAAAQDRDL
                     ALAAMQSSEAAVKSFEDQVRAAEAQVAVANANRKQLDVQQSDVAATRAQLQQARADKA
                     TADVRVSYTKVFAPMDGIVSVRAARQGEVLQVGEPIVTVLDVDRLWVQADVEETYIDR
                     VGLNQKIKVQLPSGNVLEGTVIFKGVESDFATQRDVSRTKRDIKTFEIKVAVPNAGRT
                     LMSGMTATVLLPPLPSDQSWWRKL"
BASE COUNT         4679 a         5577 c         5760 g         4783 t
ORIGIN      
        1 tgcttcggca agccgccgcg cgcagcgtcg actccctctg ggagaaaatc ggtcaagtcc
       61 tcaacacggt ctcctcgaca gagtgcacac actacttcgc ttcttcggga tatgtgtgca
      121 cttaaatcaa aaacgctcta atacataggc atgctgctgc gtacatcgaa taactatcga
      181 gcgcgcgatg ccgcatcgca atcgttacgc cgggcgaatt tcagacgtgc agtgcatgca
      241 cttcgtcgcc tttatcggca ctgcgccaag gcagaagggg cagtcgcgag ttacgggtgg
      301 cgcgggggca ggcgggggag ccagacgatt tacctgacgc accagaagga atacggcaaa
      361 agccacgatc aggaaattaa tgatcgtatt caggaaaaga ccgtagttta aggtcgctgc
      421 gcccgccgct ttggccgccg aaatcgtggg gtaatgcgtc tcagtcaaat taataaaaag
      481 atttgaaaaa tcgacgtggc cgagaagcct gccgatcggc ggcatcatta catcctgcac
      541 gaaggaagtg acaatttgcc cgaacgccgc gccgatgatg ataccgaccg ccatatcgag
      601 tacatttccg cgcatcgcga attctttaaa ttctttaagc atgagtctct cctttttgag
      661 gtggagtgat ttcgatggga cctaatctgt tcggagagat aaaacggctg ccaggagccc
      721 ccgcagtgca agaacgccgc cgacagctgc caaggctagc cccacccagc gaaacgttag
      781 aaaaacaaac gtcagcatgg cgattcctac gatcacgagg gaagcaccgc ccacgcgtgc
      841 aacggtgcgc tccggcgttc gagccgcgcg tgatgttttc aaccacgtac gcactcccca
      901 gtaaataagg accgctccga gtccaagcca ctccggactg cgcggatcga aattaacggg
      961 attcaggaat cggttactca atcccagcca aacgaggaag ccgccgagca ggatgaagat
     1021 aaactcggtc atcatccgaa ataaattggc cggagtcagc atgctcgtct aaaaacgggc
     1081 taggatcgct caatctttac agattttagt tcgacaggcg ttttcggttt gtcgcccgcg
     1141 ccgcgcggca gttccgaaat tttcgtggcg atatccattc cttcgacgac ttcgccaaag
     1201 atcgtgtgtt tattatcgag ccagggcgtg ggagccacgg tgataaaaaa ttggctgcca
     1261 ttcgttcccg gtccggcatt ggccatggaa agctttcccg gcttgtcatg cttcaacgaa
     1321 gaattgaact catcgccgaa gcggtagccc gggtctccgg ttccagttcc gagcgggcat
     1381 ccaccttgaa tcatgaattg cggaatcacg cgatggaaaa taaggccatc gtaaaacgga
     1441 cgcttcactt tttgtccggt cttcggatct ttgaattcct tggtaccttc agccaggccg
     1501 acaaaattac caacggtttg cggcgcttct ttttcaaaaa gttttacgac aacggttccc
     1561 tgtgttgtgt cgaatattgc gtatgttcct ggtgcacggc tcaagatgac ctcccatgga
     1621 tgacaaaata tgctcgcatt attacccatt ccgggcgcgt agaccagcgc cgctcgcaat
     1681 aatatgcggc ctcgattttt atcgaagcgc gcgcgcgtgc cgtaatgcta aacttccgcc
     1741 ctgcgcgtgt tgcatcgtat caccatgatc gagaatctaa tggcaggcag ggagaagaac
     1801 acttgaataa cgtgatcatt attgggagcg gctgcgcggg aatgacggct gcgatttacg
     1861 ctgggcgtgc caatctgaaa cctgtcgtga tcgacgggag ggaacccggc ggccaactcg
     1921 cactgacgac gatggtcgag aattttcccg gatttcctga cggtatcctg ggcccggacc
     1981 tgattgaaaa catgcgcaag caggcgcagc gattcggcgc ggaatttaaa tccggcgcga
     2041 tcacggacgt cgatctaagc aagcggccat tcaagattac agcgggtaaa gagacttatg
     2101 aagctcaggc gctgattatc gccgccggcg cgacagcacg cctcatgggg ctggagtccg
     2161 agcgcaaatt gatcggttac ggcgtttcca cgtgcgccac gtgcgatggc tatttcttcc
     2221 gcggcaaaga tattgccgtg gtgggcggcg gcgattcggc catggaagaa gcgaattttc
     2281 tttcgcgatt tgccagcaag gtatatctgg tgcaccggcg caatgaattt cgcgcgtcta
     2341 aaatcatggt ggaccgcgcg cgcgccaatg aaaaaattca attcgtcact ccggttatcg
     2401 tcgaagatat ttcggacgtt accaagggcg gcgttgagaa tatgaaattg cgcaacgccg
     2461 aaaccagcga gttatcgacg cttgccgtcg aaggcgtgtt tatcgccatc ggccacgatc
     2521 ccaacacaaa agtgtttcgc ggcaagctgg acatggatga aaacggctac ctcaccacac
     2581 atcacggcag ccagacgaat gttcccggcg tctttgccgc gggcgatgtt caggaccatc
     2641 attacaagca ggcagtcact gcagcgggtt ccggttgcat ggccgcgatc gatgcggaga
     2701 aatttcttga ggcccataag ggcgcgtaag actgcacggc atttcacagc gttgcccgta
     2761 caacttccat gttacgcgcg ctgctcctcg aactcgcaaa aagttcccgg cttcgccgct
     2821 ggattacttc ccatggagta acgcgacgca tggcgcaccg gtttgtgccg ggcgaggaac
     2881 ttggcgctgc gatcgaagcg gtgcgcgcct gcaaccaggc gggaatgacc gcaagcctcg
     2941 atcaccttgg cgagaacgtg gtcacgcgcg aagacgcgga acgcgcgcga gcctcttaca
     3001 ccgaagcgct ggaacgcgtt gcggcggacg acgtggactc caatgtctca ctcaaactta
     3061 cgcatttagg actcgatctg ggtggcgaat tttgcgcgga gcaattgcgc atcattctcc
     3121 gccgtgcgca agaactcgga aattttgtgc gcgtggacat ggaagggtcc gcatacacgg
     3181 atcgaacgtt gcaaatggtc aaacaggccc gcgtggaaac cgccgccgtg gggaccgtca
     3241 tccaggcgta cctttaccgc agcgaagaag atatccgtga gttgctggcc atcggctgcc
     3301 ggattcgcct ggtgaaaggc gcgtacaagg agccatcgca aatcgcattt ccccgcaaaa
     3361 aagatgtcga tgccaatttt ataaaattaa tgaaaattct tttgctcagc ggaatttatc
     3421 acgcgctggc tacgcacgat ccgaagatga tcgatgccgc cacgcgtttt gctgcggagc
     3481 aaaatatttc caaggataaa tttgaatttc agatgctcta cggcattcgc acggatttac
     3541 aaagccagtt ggtaaagcag gggtatcgcg tgcgcgtcta tattccgttc gggcgggatt
     3601 ggtttcccta tttcatgcgc cggctcgcgg agcgtcccgc gaacctgctc tttttcgcca
     3661 agaatttctt tcgcggagcc gcgcctgcgg cttaagctgt ttatagaatg aaggccgcgc
     3721 cctctggagt tccttcattg tgagccataa ctgagccata acaatgatcc aaaataaatt
     3781 catgcgcgcg atggtgctcg atcaaccggg aaaacctcta acgcttcgca acctgccagt
     3841 ttcacagccg ggaagccatc aagttttgct ccaggtgctc acctgcgccg tctgtcgcac
     3901 cgatttgcat gtggtggacg gcgaattgcc cgatccgaaa cttcctctca tcattggcca
     3961 cgaaatcgtg gcccgcgtag tgggcctggg agaaaacgtg agcctgcttt ccgtgggcca
     4021 gcgtgttggc gtgccctggc tcggctggac ctgcggcaaa tgtttttatt gctgtacggg
     4081 aagcgaaaat ttatgcgtga atgcgcgctt taccggctac acactcgacg gcggctacgc
     4141 agaatgcact gttgcggacg agcgctactg cgtccctatt ccagaatcct attccgatgc
     4201 tgaagcagct ccactcttgt gcgcgggctt gattggttac cgttcgcttg tgcgggcagg
     4261 cgatgcgaag cggctgggta tttacgggtt tggcgccgct gcgcacatca ttgctcaggt
     4321 ggcggttcat cagggacgca gcgtctatgc cttcacgcgc gccggcgaca aagaagcgca
     4381 aagttttgct aaaaagttgg gcgcgatatg ggcgggagca tctgaggagc gtcctccaga
     4441 cgaattggat gccgcgatta ttttcgctcc ggtgggggcg ctggttcccg cagcgctcgc
     4501 cgctgtgcgc aaaggtggaa aggtcgtctg cggaggcatc cacatgagcg acattccgtc
     4561 gttctcctac gatctgttgt ggggagagcg atcggtaagt tcggtagcga acctaacgcg
     4621 cagggacgcc gctgagttca tgcagatagc cccgaaagtg ccggtacgca cgacgactga
     4681 gaattttaag cttgaacaag ccaacgaagc gctcaagcaa ttgcgaaacg gaaaagtgca
     4741 tggtgcggcc gtcctgaaat gctcctgatt ttttgagaaa cacggtcgct cattgcattc
     4801 cgccaatgcg tgctatactt ttttcgggga gaaactggct tgtgcagttg ccagtttggt
     4861 gatataaacc gatgctttcc aagcaaatgt taaaaggcat tatacttttt aagcgataga
     4921 tattccccga aggagagtcc cgatggcgta cgtaatcgct gagccctgtg tcggaacgaa
     4981 agacaccgca tgcgtcgacg tctgtccagt cgattgcatt catccgcgta aggatgaacc
     5041 taaatttgag tccgagccgc aactctacat tagccccgtg gaatgtattg actgcggagc
     5101 ctgcgtcccg gtttgcccgg tgacggcgat cttcgctctg gaagatctcc cggaaaagtg
     5161 ggctaacttt acgccgatca atgcggactg gtacacgaat caaggttgat ccggcccgtc
     5221 cgaaaacggc aaattttcga gctgggtttt cgtgcggcgt tcttttgttg cagaaggacg
     5281 ccgcattttt ttgccagcgg agttccgtcc tgcctgaatg ggtgaaggca ggattgcgtt
     5341 cataattcat attcttggct ccagcaacaa gaatttgcat cctacctgtg tagttacaaa
     5401 ctcgtcttcg ggtatatgat gcccccatag agttacccgg gatccggtta gaggagccaa
     5461 acattgtcat ccgcgcgaac gcctaaatcc ttggaagtcg cagaaattcc tgccaatcgc
     5521 caggaacccc gcacaaaaat taaaaataat atcgtacctg cggtagctac gattgccggg
     5581 accgccacag ctgtacctcc ccatgtgctt tcgcgcgacc tcgtgaaaaa cggaattggc
     5641 cccgtgttta atctggaagg gtcgaagctg gatgcgattc gtgaaatcat cgataattcg
     5701 gcaatcgatc agcgctatag cgtgtttccc tccgattacc tgattgagcc gcgccctctt
     5761 gcgcaaatta atgccgagta tcaggaaaaa tccgttgaat taggattgca ggtagccgag
     5821 cacgcattgg cacaggcagg catggttcct accgacgtgg atttgctgat caccgtgtcc
     5881 tgcacgggcg tcatgattcc ttcgcttgac gcacacatcg ccacgatcat gggatttcgc
     5941 cccgatgtgc ggcgccttcc gatcacggag ctcggctgcg cggccggcgc cgcgggcttg
     6001 gcacgaactt gggaatatct gacggcgttt ccggatcgca cggcgatgtt gatcgcggtc
     6061 gagcttccta cactcacgtt tcaacgtaaa gacgcctcac aagccaactt gatttccgcg
     6121 gctcttttcg gcgacggcgc cgcgggcgtg gtggttacgg gacgcgaagc acccggcccg
     6181 cgcatcctgg cgtcggaatg ttttctgttt cctgattcgc tcggggcgat gggattcgat
     6241 ctgcgcgaca ccggtttcca tatcgttcta tcgaaggatg tgccggaaat aattcgcgca
     6301 aggatcaagg gtttggcgca gggatttctt gcacggcatg gattgcgcca ggaagatatt
     6361 tccgcgtttt tgctgcatcc gggcgggcaa aagcttttgt cctacatgca ggaagaattg
     6421 ggattgaaac gctccgacac ggaagtttcg tgggatattt tgcggcggta cggcaacctt
     6481 tcgagcgcca gcgttttatt tattttgaac gagtggctgg tgcagaaaga aatgccgtcc
     6541 ggcagctacg gactcctgat ggcattcggt ccgggattca cggcggagat gctcctgctg
     6601 caatggccat gacatttgcc tatatctcgc ttcttgtcat cgtaggcatt ggccgcttgg
     6661 cggagttggt catttcgcgg cgcaaccaac gccggcttga aaagcagggc gttcgcaaaa
     6721 ttcacgaacc gctttttcga tttcttgtgc tcttgcacgg ctccattctt gtttgcgccg
     6781 ggctcgaagt ccttttcctt catcgtccac tcattccatc gctcgctatt tcgatgggaa
     6841 ttgtatttat tctgtccaat gtccttcgct ggtgggtgat ctggagcatg gcggggcact
     6901 ggaacgtgga aatcatggac tcagcccgcg tgggggtggt cagctcaggg ccatatcgct
     6961 gggtgaagca tcccaattat gttggtgtga tattggaagt gttttccctg cccatgatgc
     7021 acacggcctg gatcacagcg atcctcggga cgctcggcta tatggaaatt attcgccaaa
     7081 gattaaaact cgaaaatggc gcgctgatgg ccagtcccga ataccgtctg atcatgggca
     7141 acaagccaag tttttttccg cgcattttta gccaaggacc aaaagaccca gcgccagagc
     7201 gcacccgtgc aggactttag tagcacaggc ttcagcctgt ggggtttagt ccctgcaggg
     7261 ccaaaacccc accggctgta gcccgcgcta ctttttccgg ctcctgtcaa ttcgcggaat
     7321 tttcctgctc gccggaattt caccggcgtc gaaatcagcc agccattccc gcacagtcac
     7381 gcgcaaagtt tcaatttcca ggtctccgta gatttccgga aatcgatcca gcttcagaag
     7441 cccctcgcga agcaaattcc gcgtgcccaa taaatttgca cgggagtgat gatgaaacgc
     7501 ggccgcaacc tgaatcaatc cctggagaaa tattttctcg ggattgggcg tcttcagcca
     7561 cacttcttcc caatgctcgt gagcatcgta aaatcgcgct gagttaaacg cttcgagccc
     7621 ttccttgtac agccggtctt tctcgtgagc atccatgtgt attttgtgtg ctttaaaaaa
     7681 tttaataaat atgcgttcat cataccaaac gcttgcagca tccgttcacg ccggcactga
     7741 agttgtagtt ccaaatggag tgaatcgtga atatgaattt ccctctttgt tactgcccga
     7801 gttaatatgg ttagccttcg caaatcagag gttcttgcgt gtcgaaattc aatttagtgt
     7861 tgctcattca tgcgcaccag cccgtcggca attttgacga cgtgatggag cgcacttacc
     7921 agcaatcgta tctgccgttt gtggaatgtg tcgcgcggca tccacgcgtt cgcatggggc
     7981 tgcattactc ggggtcgctg atcgagtgga tggcggaaat gcatcccgaa tacatccagc
     8041 gcgtcggtgc gctcgtggct cgcggccagg tggaagtcgt tggcggcgga ttttatgagc
     8101 cgatcctcgt ggcgattccc tatgaagatc gtatagatca gatccggcgg caaagcgatt
     8161 ttgtggaaaa actttttggg aagcggccac agggcgcatg gcttgcggag cgcgtttggg
     8221 aaccgcaatt gcccacggca ctggcggaag ccggtgtgga ttacacgctg gtcgatgaca
     8281 gtcattttct gacggctggc cgcgaaattc cggagctctt cggctattac gttgccgacg
     8341 attgcggcaa aatggtgaag gttattcccg gcttgcagga gttgcgctac ctgcttccat
     8401 tcggcaccgt ggaggattcg atcgcattcc tccgccgatc cgccaccgat catccgggca
     8461 gcatggcttc catgggcgat gacatggaaa aattcggcgc ctggccgcat acctatgagc
     8521 attgctttcg cgatggctgg ctcgaccgct tcttcagcgc catcgaagcg aatttcgagt
     8581 ggttggagat ggtgcctcca ggagaggctt tggcgtcgca cgcgcccctc ggccgcgtcg
     8641 atctgccaac cgcgtcctac accgaaatga tggaatgggt tttgcccact tccgtaagac
     8701 aaaaatttca ttctctgctt gaagaatttt ccgggcgccc ggacgtgcgc ctgttcctgc
     8761 gcggcggttt ctggcgcggc tttttcagca aatacgcaga ggccaatcta ctgcacaaga
     8821 aaatgctgcg cgtttcctct aaattgcgag gcgatggaaa aaagagttcg cggcgtggca
     8881 cgaagggggc cgtccttcgc tcgcgggcca ttacgcatct cttgcgcgcg caatgcaacg
     8941 atggctattg gcacggaatc tttggcggcc tgtatgcgcc gcacctgagg acagcactct
     9001 ggcgtgagct cgtgcgcgcg gaaaccattg ccgacggcct gcgtcacgga gcgaagccct
     9061 atcagaccat gacgcggctt gattttgacg ctgacggcag ggaagaaatc gaaatcactg
     9121 cgcccgaatt tgcagccctg atcaacccat cgcgaggcgg cacgttggaa gttctcgatt
     9181 tccgacccag cgccgtcact ctgatcaatt cgctacaacg ccgcgtcgag gcctaccatt
     9241 cccgcttgca gaatatgtcg cagtctgccg gcaaagttgc ttccatccat gatcagacgc
     9301 tcgccaagga acccggcctc gaaaagtatt tgaagtatga ccgctggcca cgaaatactt
     9361 ttcggctttt gctgttcgcg ccgggaaaaa cacatgcgga ttttgaagcg cttcgtctgg
     9421 aagagagcgc gccctttgcg ggcggggatt accacgtcga tcaagccagc gccgaagagg
     9481 ttacgctgac caaggaagca ttgctgcgcc aggtcaatgc gggcgcggat ctcaaatcta
     9541 ttttggccgc gacaaaaatt ttgcaatttg aaaagcagga acatggcttc gatgttcgct
     9601 gcaggctgga gctcgcgctg aaagatgcgg agtacatggc ggaaggcgcg gacgatgccg
     9661 tgaacattct tgtgggattg gaaatcgtga tcaatcttct tgcgcccaat gttccggatc
     9721 gctatatcga atttgcaggc gttcgcaaac ctctggagtg gagcggcgtg gtcgaagaaa
     9781 agaaattacg gattgcggat gaatggcaaa acgttgctgt ggaagtggaa gctccgggcg
     9841 catgccattt ctggatttct cccattcaaa cggtttcaga atccgaagag ggtttcgagc
     9901 gcgtctacca gggctcgcaa attctcggcc tgtggaatgt gaagttggat ccgtcgaaac
     9961 cttggagcgc ggaaacagtt ttgcatgttt ccaaggcgca caatccctga cccagttctg
    10021 caatcaaacg gcaaataaaa ggttttccgc gaacggccac tgcttatcga tccactgtga
    10081 atcgcagcga aatccgttgc gttcagccat ggcagggata tcttccgcgc catatttgtg
    10141 tgagctttcc gtccagatcg tctcatcctt caaaaatgaa acgctgcatc ccgcgcctgg
    10201 aattttaacg gtctgatcgc gcagggaacg caaatgcatt tccacgcgct ggtgcccttc
    10261 actgtagcga gcctcgtgcc ggaactccgt gaggttgaaa ttcgcatcca gttcgcggtt
    10321 aatgcgcgcc agaaggttca tgttgaacgc ggctgtcact cccaaggcat cgtcgtaagc
    10381 agcgaataat tgcggcatcg gttttataag gtcagtcccc agcagcagtg catcgcccgg
    10441 aaacagaatg cgccgcactt cggaaagaaa gcgttcgccc gcatcgcgat cgaaatttcc
    10501 aatcgtgctt cctaaaaaca aaacgagcag gtgatcgttt tcctgacgat gcgcggaagc
    10561 agcaagcagg ccatcgagat aaggacgctc aaatcctaca atgcttatgc attcgatttg
    10621 cccaagttca ctctcgcagc gggccagcgc cgtgggtgaa atttcaatcg ggcaatacgt
    10681 tgtgcgctgc cgcagggcaa gcgcttccag aatccagcgc gtttttttgc cgctgccgct
    10741 tcccaattcc gccaccagga cctgcggtgt gagtcgcgaa actatttcag ccgcgtgatc
    10801 gtgcaaaatt cgttcgtcgg cccgtgttaa gccgtattcg ggcagcaggg tgatcacttc
    10861 aaaaagcgcc gagccgattt cgtcatacaa atatttggag ggaagttcct tttgtccacc
    10921 gcgacacaat ccggcgcgta cttctcgtgc aagttccgat cttgaatgtt gcgcggcgac
    10981 tgccgttgcc atgatgggtc ctcgattcta gtcttggcct aaaattaccg ttcaacacat
    11041 cggaatttgg catacacgta gggataatgt ggctggaacc aattgcggaa ggaccggcgc
    11101 agcatgcatg cggccgtgcg cggcgaaccg cccttcatca cgaaatgctt cccatcaaag
    11161 aaatccgcgg aatatccggg ataaaaagaa aatggctgaa aaccggaaaa cggctcaaag
    11221 ggcgtgcgcg tccattccca accgtttcca acgagatcgg caacgccgaa tgcgctgttg
    11281 cccgccggat acgatccaac ggaaacggga tcccatcgct gaaaatcaaa attgccatgc
    11341 tgggttgacg gagcgtccga gccccaggga taggcacgtt cggtgccttc cggtgtatcg
    11401 agcgctgctc gatgccactc tgcctcgctg ggcaattctt tgcctaccca gcgtgcataa
    11461 gcggaggctt cgtcatggct cacgtacaca ggccaatcga gaggcaacgg aatttcagcg
    11521 aacatcgcgc gataacccca cgtatcgccc tttggaagcc agaatgccgg ggcgcggtgc
    11581 ccttgggagg aaatccaact ccacccggca tccgtccaca gatcgcggcg ctcgtatccc
    11641 ccatcgcgca ggaactgcag gaaatcacga tttgtcacgt taaacgcatc gatgcggaac
    11701 gcaggtacct gaacgcgatt ttcctcaaac tcattgtccc aaccgaatgc gccgccctcc
    11761 ttgcgagaga ggccaagcgt agcggtaccc tcaggaattg taatgcttcg ttgcaagtgt
    11821 gctggagcga agggcgctgg cggagtatgc tgagcgagtt tgcggttccc cggcaactgg
    11881 tgcagcatgt agcagagggt ctcagaatgc atcagacgat gttcgatcgc cacgtcgagc
    11941 aagcgcccaa gatcgagacg ccccatctct ggatctttca cgccggcatc cctgattgca
    12001 gcgttcagca aatcgcgaag gcgctggttg tattccctca cccgctcaac cgtaggccaa
    12061 tccccgggct gatccatggg cagtcctccc tccaccggat cgatgccaaa cgcgaacagt
    12121 ctgtcaaagt cggggccgaa ggaagtgagg ccaagagttt cacgcaaaag attccagtca
    12181 aaggcttcga ggtgtcccag gtaaaaaata atccgatggc gttccgcaac ggggcggtca
    12241 taaagcgcgc ctggatgaac aattccgaag agttcttcgc tgcgggcccg tgcctcctga
    12301 agacgagcaa aaagccgatc ggcacgggaa gattgttcgg ctgcggtcga ggtcattgtt
    12361 caccagtgtg tttagatgta tttgccgcgg gctggattca ccggcgaagc agaacaccga
    12421 ctaccaccat gatcaggggc cgccaaattg gaagtcaata catgttctca gggagggcca
    12481 aggtgcccgt tttggggcac ggcggcgtaa aggcgtgact tcgccaaccg attcattaca
    12541 agacggatac gctcttacat ttcagaatgc aagttaccgg ttgccgtccg gtagcactct
    12601 tatttccagc ctggatctcc atgtcgcgcg gggcgaaacg cttatcttgc ttggcagaag
    12661 cggttcgggc aaaaccacta ccttaaaact aatgaaccgg ctcctggatg ctaccgaggg
    12721 cgagatcctg attgccggca agcctattcg taccctggac ccgattgagc tacgaagggg
    12781 aattggttac gtaattcagg acgcgggact cttcccgcat tggacggttg agcgaaacgt
    12841 cgggctggtg ccccggctgg aaggttggcc ggaggagaag atagaggctc gcgtccagga
    12901 aatgctttct ctggtaggcc tcccgcccga ggaattcgcc gaacgccgtc cctccggcct
    12961 ctcaggtggc cagaaacagc gcgtaggagt cgcccgggcc cttgccgctg atcctcccat
    13021 cctgctcatg gacgagccat tcggcgcgct cgatcccatt acccgcgcgg accttcaaaa
    13081 tgagtttcga acgctggcgg cgcgtctcca gaaaacgatc gtgttcgtca cgcacgatgt
    13141 tcgggaagct ctctatctgg gaacccgaat cgccgtcctc gatcaggggc gactcgtagg
    13201 cgtttatacg cctacggaat tccttaaagc caaagaaccg gagatccatg cgtttgccgc
    13261 gtctttgcat tcgggtgcgg caggtgaggg ggcggaatga aatggtatga gttcctgctg
    13321 cacaaccgcg cagaggtttt tcaacgaatt atcgagcatc ttgggctggt agccgcttcg
    13381 atggccattg cgcttgcgat aggcttgcct gtgggaattg cgctcgttcg gcgggcgaaa
    13441 ttgcagcggt gggtgatcgg aggggccaat gtattacaaa cgattcccag ccttgcgctg
    13501 tttgggttcc tgattccagt tccattgatc ggcggggtcg gggcaagtac ggccatcgtc
    13561 gccttgtttt tgtatgccct cctgcccatt ttgcgaaata cgtgcattgg gatcattgag
    13621 gtcgatccgg cagtcgtaga agctgcgcgt ggcatgggca tgactcctcg acaagtactc
    13681 tggcaagtgc agctgccact tgcggcgccc gtgctgctcg cggggattcg cgttgcgact
    13741 gcgatgtgca tcggaattgc taccgtggcg gccgcgattg gagcgggcgg cctgggagtt
    13801 tttatctttc gcgggctggc gatggtgaat aatcaggtga tcctcgcagg ggcaattccc
    13861 gcggcgggta tagcaattgc cgtggattcc ggattgggtt actttcagaa aagtgtcgac
    13921 aaaagatgaa tcgctcgccc ggctggacca cgctgaaacg cagagtaccg cgatttgcga
    13981 cttgcatgct cgttcttggc gtactggctt cgctttttgc cgcgagttgc aaaaaacaga
    14041 gcggcggcga aaccatcgtt gtaggctcaa aaaattttac ggagcagatt atccttgcgg
    14101 aattatttgc gcagcaaatt gaagcgcact ccgcgctgcg tgtggagcgg aaactgaacc
    14161 ttggagggac atttatttgt cacgatgcgc tggtttccgg aaaaattgat ttgtacccgg
    14221 agtacacggg cactgcgctg atagccgttt taaaagagcc gcctcaaaac aacccctcgg
    14281 aagttttccg ccgcgtgaag gaagagtatc agtccagatt taatgtcgag gaaatgccac
    14341 cgcttggttt caacaataca tttgcgatgg tcattcgcgg cgacgatgct gaaaaattgc
    14401 atctccgcac ggtttccgac attgccccct acgccccgaa atggcgcgcg ggattcggtt
    14461 acgaatttat ggagaggcca gacggatacc aaggctggat agaagcctat gggattcatt
    14521 ttgccaatac gcctcgcatc ctcgatcttg gattgctgta tcgcgcactc gcggataaga
    14581 aggtcgatct cgtggccggg aattccaccg atggagtgat cgcgtcctta catatggttg
    14641 tattggagga tgatcgccat tattttccgc cctatgaggc tgtgcccctt gtgcgccgtg
    14701 caacgctcga acggcatccc gaaattcgtg acgcgattgg cgcgcttgcc gggaaaattt
    14761 cagtggatga aatgcgaacg atgaattacg cggtcgatgg cgaacagcgc gatcccgccg
    14821 atgtcgttcg cgcgtttcgc aaatcgaagg gtttgtaacc gctgcatggc aaaagactta
    14881 aaaaaatcca ttatatagga ttttccgacc gatccgtttc catccgggct gttgacattc
    14941 caagtactta cgtttcctcg taaagagctt tccgggttta gccgatgtgt gtacaggatg
    15001 aaagaaaaca ggatcgaacc caggttcaat atgaacctgc cgattgtagc ttggtgggaa
    15061 gacgaggccg gcaaacaacg tttttctcac ggaaaattgg aagatatttc caatgccggt
    15121 ttgcgcttca aatctcggga gccaattcct gtcggaacca aactgcttct gcgcacgccc
    15181 atgggagaat acgtcggcgc tgtcgtacgt tcgagccaag atggggagga atttaatcta
    15241 ggcatcaaaa aaatctccga gcggaagcct tcaggtacac acgtcaagct ttagccgtta
    15301 ggcataaact catttatttg aattctaatg acggagttgc ccgcggcaat tcgtctctgc
    15361 tagagtgcgt gcgtatgcca acggctcaaa ttcaaaagtt caaataccga atttgggttt
    15421 gtggaattag taccttcctt tgacatgacc ttcgataatc gaaagctcac caatttcgcg
    15481 aaagctcttc gcggccggcg catcggcgtg gccggtgatt tcatgctgga ccgttacgtc
    15541 tggggttcag ccacgaggct ttcgcccgaa gctcccgtgc ccgtcgtcga ttttaaggat
    15601 caaacgcagg tactcggcgg tgcgggaaac gtagctcgaa atttagctgc gctgggcgcg
    15661 aaagtccttc ccttcggagt ggttggcgaa gatgaggatg gtcttgcgat tcgagaatta
    15721 tttgaagaag acggcacggc caccaagggt gtggtcgccg acgcatcccg cattaccacg
    15781 gtaaaaactc gcatcgtcgc caaaaatcag caaattgtgc gtgtggatcg cgagcgccgc
    15841 gaaccaattc atggcgcgct ggaagatcgc ctgggacgcg caatccttaa ggcgctgccg
    15901 cgcctcgacg cgctcgtcat ttccgattac gacaagggcg tgattacgga tatgctcgcg
    15961 gatcgcgtgc ttaccgagtg ccagcgtcgc ggcgtccccg cgctggtgaa gccaaaagag
    16021 tcgcggctac tgttttatcg tggcgcatcc atgatcgtgt gcaacgcaaa agaagcgcac
    16081 tttttcatca gccgcaaatt tgaggatgat gaatcggttg agcagggagg ccgggcactc
    16141 ctcgcgcatt tcggatgcgc cgctgcggtc atcacgcgtg ggcgcgaagg gctcaacgtg
    16201 tttgagaaag acgagaagaa aagttttcac gtctcagcca ccagcagcga aatcagctac
    16261 gatcgcgtcg gcaaagcggg agtcgaacgc tcggcgcacg gcaggcaggt tttcgatgtg
    16321 acgggagccg gtgacacagt gctatcggtt cttgcgcttg caattgctgg caggattcct
    16381 attcgcgagg cagccgtgct ggcaaatgct ggagcaggcg tcgtggtagg aaagcttgga
    16441 acttcgacga ttacgcccga ggaattattc gccgctgtgc gcgaacttcg ttgatcttta
    16501 attcgtaatt ttttaaatca cgatcgggag aggggtttac gtggcatagg cttcagcctg
    16561 tggaattaga tcttgcttgg actaaacccc acaggctgaa gcctatgcca cagacggcaa
    16621 aatcagttta tggcgggggg aaactcatcg attgatcgga gcgctcaatc cacagttgca
    16681 cctcgcgatt tcgcgcggat tttatggtct cctcagcttt cggaaatgta ccgtattcgc
    16741 tgagttcaat caccacaacg actcccgtcc cgcccttggg aggcacttcc gccgtttcaa
    16801 tttgataact tttgatggga ctgtaataac cgttcggccc ccagaaactc aggaaatccg
    16861 gataggagaa gccgggatgc gggtgccaga tttgataagc ctgttgcgta tcgcccgcga
    16921 cgatcgcgtt catgaagcgg gtagcggtat gcttttcggt cgagtatcta agtaaaaacc
    16981 aaatagcccc ggcaattaag agcaccagaa ccacaccact gatgacgtag cgcaaagcct
    17041 ttgatttagg ttcttcggcg cctgcgtcga gtaatcccat gaacatttct cctttaggca
    17101 tcctaaatct gcctcttgaa taaagcaacg gagagagcaa cgagcacaat cgagaatccg
    17161 gttaggatca gaacatccga atagatgccg gagaaacctg tgtttttcaa aagcagcgcc
    17221 ttcagcgcgt gaaccgcata ggtgaaggga tcgatgtacg aaatgaaccg caaccagaca
    17281 ggaaatccct cgactggata aatcgctccc gaaggaaagt agagaagggt attgagcacg
    17341 ccgaatattg cacgcggcac aagcggatcg ctgacgcgaa ccatcagcaa aaacataaag
    17401 ctgatcatgg caatcgacgt gatgaccaac acgctcatca gaaaaagcag tcgtactgga
    17461 tcccatagat gatcgatgcc ggcaatcaat ccgccaataa tcgtaatcgt cgttccggcc
    17521 atcagccctt taatcgcgcc ggaaaaaatc agacccaaaa ttaattctgt tttggtgatc
    17581 ggcgtgacta aatatccttc gtgcaacccg cgcgatttgt cgtcaatgaa cgtgatgcct
    17641 ccgccgatca tcgccacgat aaaaacggac atagcaatcg atcccgaaag caaatatttg
    17701 atgtactcga tgtacggata tagttcgaca acctgtaaat tcacgagagg attcaggcgt
    17761 ggcaatggta cggtcaagcg ctgccccggt tgaaacgggt tcagcccctg attgatgtcc
    17821 ttcaccatat cctgcatgcg cgtgagaatt tcggaggtga cgaaattgtc tgtgttgtct
    17881 tcgatgaaag cgacttgcgg ccgatttccc tggttcacgc gccgcgaata atccacagga
    17941 atatagacga cggcgcggac gaatccggcg cgcaaatcga caatggcatc cgagacggaa
    18001 ttgtaattct cgactttcat cgtctccggt cccgctacaa tcgcgttaaa gcgttcgcgc
    18061 aagtcgcgcg aataaaccgt gcggtcttca tccaccaccg cgaccttgat tcccttaatt
    18121 ttcccgccaa acgcgtatcc gagaacgatc agctgcatca gcggaaaaat catggagctc
    18181 atcatcaggg cagggctgcg gaaaaatttc cgcatgtcgc gttcgatgat ggcggagatg
    18241 cgctggaatt tcattggatt cgcaatgatc cctatttgta caaatggctc atgtcgcgct
    18301 tcatggtttc ggaagcggca tcgcgcagtt gtcttcccgt gtagtacagg aaaacgtcat
    18361 cgagcgtcgt gccttgcacc gaaacgcgtc gcacgttcac gccgcgctct cgcgccgtat
    18421 ccatcagttc gcgcacggtc agcgggccgt tggtggtcga gatgtgcgtg aagtcgtcgt
    18481 tcacagccac gctggccacc cctttaagct tttccagagt cgcggtccaa tccgccggcg
    18541 gattttcaaa ttccgcttcc acaatgtcga cggccggaac gctgtctttt aattttatcg
    18601 gagtatcgag cgcgacgagt tgtccatgat ccacaatcgc gattcgatcg cacagtttgt
    18661 cggcttcatc catgtaatgc gtggtcagca aaatggtgag gcccgcggat tctttcaact
    18721 tggtgatcat ttcccatacg gctgtgcgcg acacagggtc gaggccggtt gtgggctcat
    18781 cgagaaacat gattttgggc gaatgcacca ggccgcgcgc aatctccaaa cgccgccgca
    18841 taccgcctga aaagctgccc accagtttgt cgctgaattc cgtcaaccct accgattcta
    18901 aaagttgagg gactaagcgc tcgcgcgcgg aagtcgcgag cccgtagagc ttcgcgtgga
    18961 tattcatatt ttctcgcgct gtcaactccg gatcggacgt gagcgcctgc ggaatcactc
    19021 cgatggcgtg acgaacgccg ttcgcatcgc ggacgacgtc atgcccgccc acgtttgcgc
    19081 tgcccgaagt tggcggcgtc agcgtggtca tcatgcggat caacgtagtt tttccggcgc
    19141 cgtttggtcc gagcaggccg aagatttctc cggtgtgtac tgcgaagctc acttccttca
    19201 cggccacgaa atcgccaaat tgttttctca atttggtgac ttcgatcgca ttggtttcga
    19261 tgttattcat cgcgtttgat tgatggcttt cgtggcgcag gccttggcct gcggatttta
    19321 ggctttgcaa gcacgaaccc ccaggctgaa aagtctgcgc gagacctgca aaattgaagc
    19381 ttcagcggct aaagccgcac tcgttttgca ggacttatgt cgtggctaaa gccacgaccc
    19441 acaaagattc tgcagttctc acacagactc tgaagtccgc gccacctaaa gtttccgcca
    19501 ccaactttga tccgatggca ggggcggcaa gagcacagtt gcggtcatgc cggacatcag
    19561 cgtgcgcccc gcgttgggaa ccgccacttt aatttcaaaa gttttgatgt cgcgcttggt
    19621 gcggctcacg tcacgctgtg tcgcaaaatc actctcaacg cctttaaaaa ttaccgtgcc
    19681 ttcgagaaca tttccggaag ggagttgaac tttgattttc tgattgaggc cgacgcggtc
    19741 aatataagtt tcttcaacat cggcttgcac ccagagacgg tccacgtcga gcaccgttac
    19801 aatcggttcg cccacttgaa ggacttcgcc ctgccgcgcg gcacgcaccg aaacgatgcc
    19861 gtccatcggc gcaaaaactt ttgtgtagct cacgcgcaca tccgccgtgg ctttgtcggc
    19921 gcgcgcttgc tgcaactgcg cgcgcgttgc cgcaacgtcg ctttgctgaa cgtcgagctg
    19981 tttgcgattc gcattggcta cggcaacctg cgcctcagcc gcgcggacct gatcttcaaa
    20041 cgatttgaca gccgcttcgg aactttgcat cgctgcaagc gcgaggtcgc ggtcttgcgc
    20101 cgccgcgacg ccggcgtcaa acaaactttg cgtgcgttta taagtggtct gatcgcgcca
    20161 gagattggct tgtgcttgcg caagttgcga ccgcacggaa gtgagagtcg cctccgcctg
    20221 acgaatggac gcgctggtct ggtcatccgt catgctgcgc gttcccgacg cggaaattac
    20281 tttattttcc agcatgtgaa tgttggcttc cgcggaatcg cgcgccgtaa cccactccgc
    20341 cggatcgatt tccgcgatca ggtcgccctt atgcacttcc gagccttcat cgacattcag
    20401 ctttatcatg cgcccggcaa tttgcgagct gacgattacg tcgttgcccg taacgattcc
    20461 ggtgagcgga atactcgttc ctcttggcgt cgtagtgaga tacaccacga cggagacggc
    20521 gaacaacaca ccgacgaaga caaaaaattt ggagaatttc attcgatctc tgccccaggc
    20581 tcaagcagcg cgtgccgcga atctcgcttg tagctgacga gtaagtgtaa ggtacgaatt
    20641 tcgcaggctc aaggaatagc gggaagctct gtgttcatct gaatgctgat tggcttattt
    20701 ggcataagct tcagagtctg tgtaagaact gcaaaattga agcttcagcg gctaaagagg
    20761 ctgcggaaaa actcaacatt ttgtcattcc gagcgaagc
//
LOCUS       DASZMB010000123         3665 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4787723, whole genome shotgun sequence.
ACCESSION   DASZMB010000123 DASZMB010000000
VERSION     DASZMB010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4787723"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..272)
                     /locus_tag="VG272_05635"
     tRNA            complement(198..272)
                     /locus_tag="VG272_05635"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(238..240),aa:Lys,seq:ctt)
     gene            complement(340..1305)
                     /locus_tag="VG272_05640"
     CDS             complement(340..1305)
                     /locus_tag="VG272_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13198.1"
                     /translation="MANPVKVRLCFHDRCFDGAASAAVFYRFFRECFHSDAEFHFSGM
                     AHQARNPFPESLFDGDENAIVDFKYSSSPRVTWWFDHHQSAFLTPADAENFRQDRSGH
                     KFYRPEYKSCTKLLADVTSEKFGFDVKPLAELIRWGDIIDGAQYPTPESAVAVAEPAT
                     KLALVIESAPEEGLVSKIIPDLSTRPLAEMIELPIIAKHLGPLLEKHRQSIDLLKKRI
                     ENRGGVLYFDITDLPIDGYNKFIPYMLYPDARYTVSVLSSASRAKISLGSNPWNPDAA
                     EDNLASLAEQYGGGGHPRVAAISLDPGEVERAKQVAKDIAEKLRD"
     gene            complement(1562..2044)
                     /locus_tag="VG272_05645"
     CDS             complement(1562..2044)
                     /locus_tag="VG272_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HWF13199.1"
                     /translation="MAFCSKCGTEVGAGAAFCPKCGAAQSSGAAPAAGAVIEPVQASQ
                     SGLAENVAATLCYVLGWVTGIIFYLIDKRPFVRFHAAQSIVTFGGLHVLNIVLGIVFG
                     AGLMFRGGFGTFGIASAFYSVIGLLTFVLWILLMVKAYQHEKFEVPIAGGIAKGFSGT
                     "
     gene            2303..2386
                     /locus_tag="VG272_05650"
     tRNA            2303..2386
                     /locus_tag="VG272_05650"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2337..2339,aa:Leu,seq:tag)
     gene            2519..3511
                     /locus_tag="VG272_05655"
     CDS             2519..3511
                     /locus_tag="VG272_05655"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWF13200.1"
                     /translation="MSARAAQSAANANRDQRIGPLGIALENFDYPYPVHFMQFQMEGQ
                     VVRMAYMDIPPSALANGRAAVLLHGKNFGGYYWKNTIDALANAGYRVVVPDQIGWGKS
                     SKPDLHFSFSKLAANTASLLDSLGIQKIVLFGHSTGGMLAVRFARTYPQRVTALVLED
                     PVGLEDYRLKIPPQTDETLFQDELKNTDPEKIRAFFAHYFAHPDPDTYGPLAEVQIRV
                     AQSGEFPRWAKASANAYQMIYEQPVLYDYRLLQPPTLLVVGQEDHVAVLISYASDEVK
                     PTLGRVAELAQEVIRQVPHGTLIIVPNAGHIPHLEQPAQFHKAVLNFLAEHAGN"
BASE COUNT          855 a         1000 c         1010 g          800 t
ORIGIN      
        1 cgaatctttt tcgtctcgag gcaatagatc gtcaggccga tgagcgccgc agcctgaacc
       61 gcgagcatca ggatttggat gtgatggtca gtcattgcca atctcctgcg gaacactgac
      121 aagggcactg acacggaaat gcggggcgga acggtcaggc ggaggatcgt cgttgaagcc
      181 gcatgtttat tgtgatttgg tgaaccgggt aggactcgaa cctacaaccc gctgattaag
      241 agtcagctgc tctaccattg agctaccggt ccacgaactt ttcattttag catgacgggc
      301 aaaaattctg actgcctaac atgcgttcca cgaaaaaaat tagtcgcgta atttttcggc
      361 gatgtcttta gccacttgct tcgcgcgctc gacttcgccc ggatcgagcg aaatagcggc
      421 tacgcgcggg tggccgccgc cgccgtattg ctccgcgaga ctggcgaggt tgtcctcggc
      481 ggcgtccgga ttccatggat tcgagccgag agaaattttc gcgcgagacg ccgaagagag
      541 cacgctcacg gtgtaacgtg cgtcgggata cagcatgtac ggaataaatt tattgtagcc
      601 gtcgatgggc aggtcggtga tgtcgaaata caggacgccg ccgcgatttt cgatgcgctt
      661 ttttaagaga tcaatagact gacgatgctt ttcgagcaac gggccgagat gtttggcgat
      721 aatcggcaat tcgatcatct cggcgagggg acgcgtggac aggtctggga taatttttga
      781 gacgagccct tcttccgggg cggattcgat gacgagcgcg agctttgtgg ctggctccgc
      841 taccgcaacc gcggactcgg gcgtgggata ttgcgcgccg tcaattatgt cgccccagcg
      901 tatcagttcg gcaagcggct tcacatcgaa gccaaacttc tcgctcgtta cgtccgccaa
      961 gagcttggtg caagatttgt attcgggtcg gtaaaatttg tggccactgc ggtcttgccg
     1021 gaaattctcg gcatccgcgg gagtaaggaa cgcgctctgg tgatgatcga accaccacgt
     1081 cacgcgcggc gaactggagt acttgaaatc aacgatggcg ttttcatctc cgtcaaacag
     1141 cgattctgga aatggattcc gcgcctgatg cgccatcccg ctgaaatgaa actccgcgtc
     1201 cgaatggaaa cattcgcgga aaaagcgata aaaaacggcc gcggaagccg ctccatcgaa
     1261 gcatcggtca tgaaaacaca gtcggacttt taccggattc gccactcgat cgtaggcccc
     1321 atctcgcaaa tctacgtaat gtgaaagccc taaaagatgg ctgggacgcg cgtgaatgtc
     1381 aagacgaaaa ggaagcgctg cgatttcgag ggaatcggag atacctgctt cgtccggtgg
     1441 cctatgtgca atcgaacccc aagaatattg tggccgaagg tcgccggcgc atcggcaggc
     1501 ttgccgggtg cgccggccat atctctcgga cggcaaaggt agtatttaag gcgaaaacac
     1561 gttatgtgcc gctgaagcct tttgcgatgc cgccggcaat cggcacctca aacttttcat
     1621 gctggtaggc cttcaccatg agcaaaatcc agagcacgaa ggtgagtaaa ccgatcacgc
     1681 tgtaaaatgc cgacgcaatt ccgaaggtgc cgaagccgcc tcgaaacatg agccctgcac
     1741 caaaaacaat ccccagcacg atattcagga cgtggagtcc gccgaaagta acgatcgact
     1801 gcgccgcgtg gaatcgaacg aaggggcgct tgtcgatgag gtaaaaaatg attccggtga
     1861 cccaacccag cacatagcac agggttgcgg caacattttc ggccaagccc gattgtgaag
     1921 cttgcacagg ttcgatgact gccccagcag ctggtgctgc gccgctactt tgcgcagctc
     1981 cgcactttgg gcagaaagca gcccccgctc cgacttccgt accgcacttg ctgcagaacg
     2041 ccataggatt tcccccctcg gttgatcggc aagcaaaccg accatcatcg ggcgaaggct
     2101 atcactgctc cgctcgccac acaagctata cacaatttta atgcgagtca aaaacgagcc
     2161 ggaagcaagc tcacgtgggg atcgggcggc ccttcagact gcacgcgatt tgtgacgtct
     2221 tcaaaggtgc agccatctct gtgttagagc ctttgacccg ccaatcctca agcaccacca
     2281 aagtgttata ctggcgcgcc gcgcggaagt ggcggaattg gcagacgcgc cagccttagg
     2341 agctggtggc cgaaaggccg taggggttcg agtcccttct tccgcaagtt tcaattttga
     2401 gtagaggcaa tccggtgaat cctatcgcga gaaatctgct ttgaaaaata atcatacgaa
     2461 gtttttgtcg ctcgcagcct tttctgcaat cttgctgttc ggaagttcgt tgtaccccat
     2521 gtctgcgcga gctgctcaat ccgccgctaa cgcgaatcga gatcagagaa ttggcccgct
     2581 cggcatcgcc ctggaaaact ttgactaccc gtaccctgtt catttcatgc aattccagat
     2641 ggaaggtcaa gtcgtgcgca tggcctatat ggatattccc ccatccgcgc tcgccaatgg
     2701 acgagcagcc gtcttgttgc acggaaaaaa tttcggcgga tactattgga agaacacgat
     2761 tgacgcgctc gcgaatgccg gttaccgcgt cgttgtgccg gatcagattg gctggggcaa
     2821 atcatcaaag ccggatttac atttcagttt ttcaaaattg gccgcgaaca cggccagttt
     2881 gcttgattcg ctgggcattc aaaaaatcgt tttattcgga cactcgacgg gcggcatgct
     2941 cgccgtgagg tttgcgagaa cgtatccgca gcgtgtaacg gcgctggtgc tggaagaccc
     3001 ggttggcctg gaagattaca gattaaagat tccaccgcaa acggatgaaa cgctttttca
     3061 ggacgagcta aaaaatacgg accccgaaaa aatacgggcc ttcttcgccc attactttgc
     3121 tcatcccgat ccagacactt atggcccgct ggcggaagtg caaatccgcg tggcgcaaag
     3181 cggagagttc ccgcggtggg cgaaggcatc cgccaacgcc taccagatga tttatgagca
     3241 gcctgtcctc tatgactatc gattgctgca gccaccgaca ttgcttgtgg tgggccagga
     3301 agatcacgtc gcggtactca tcagttacgc gagcgatgaa gtgaagccaa cgctcggccg
     3361 tgtcgccgaa ctcgcgcagg aagtgatccg ccaggtccct cacggcactc tgatcattgt
     3421 tccgaacgcc gggcacattc cccaccttga gcagcccgcg cagtttcaca aagcggtgct
     3481 aaattttctg gctgagcacg ccggaaatta agacttcttt acctagcatc tatattccga
     3541 ccgcgtccgt tggcgttgcc tccaccgtgt cggcacggtt cgccgtgaac attcaccgtc
     3601 aacggcttgc ggtgccgcta cacggtgcga catgtaaagt tcgtcaagaa cgccgaattg
     3661 atttc
//
LOCUS       DASZMB010000124         9186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3676507, whole genome shotgun sequence.
ACCESSION   DASZMB010000124 DASZMB010000000
VERSION     DASZMB010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 9186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9186
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3676507"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            656..1267
                     /locus_tag="VG272_05660"
     CDS             656..1267
                     /locus_tag="VG272_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13201.1"
                     /translation="MRKLTILCVALFCLSLTASAQDSPAAFDTASPASEAVPAPAPAP
                     LLPSDRDPWQVGLGFQYLQFNVLGVRFHDFAYKVDGTRYLNNWFGVEGAVIAGFGHVE
                     GAPKVDAKSLFLGGGPHISVYNSRHLEPWVHALVGWEHLRFTQGATLGADSHAAFYAG
                     GGVDYKISATRLFWRVQGDYIGTNFGPKLSTNYAFGTGVVINF"
     gene            complement(1422..4277)
                     /gene="secA"
                     /locus_tag="VG272_05665"
     CDS             complement(1422..4277)
                     /gene="secA"
                     /locus_tag="VG272_05665"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HWF13202.1"
                     /translation="MRVETLIDQVVARFIGTKHERDLKVIQPHVNAINSLSPDFEKLT
                     DAEILEKIVQLKTQVQGRLGDISLEDPDFKERCKDALAPALVPAFALVREVGRRKLGM
                     RHFDVQLIGGIVLNEGKIAEMKTGEGKTLVATLPAVLNALTGRGVHIVTVNDYLARRD
                     AEWMSPIYRALGLTVGVIVHDLSDAERRAAYGADITYGTNNEFGFDYLRDNMKFDLAD
                     CVQRGHHFGIVDEVDSILIDEARTPLIISGPAEESTDKYYRIDKIIPKLIQDIDYIVE
                     EKHKTATLTEEGVSKTEKLLGLGNMYDPQHLETIHHVYQALRAHTLYKRDVEYVVKNG
                     EVIIIDEFTGREMPGRRWSDGLHQAVEAKEGVKIERENQTLATITFQNYFRMYKKLSG
                     MTGTAETEATEFNKIYKLDVSLIPTNRKLLREEFPDVVYRTAAEKYEAVVNGIMQEDN
                     SYAHGIRQIHESGQPVLVGTISIEKSEHLANLLKKAGIPHQVLNAKQHEREAKVVSQA
                     GRKGAVTVSTNMAGRGTDILLGGNPEAMTREYCLQEKLAIPYISAPAVIGGDGAESVT
                     APNMVLFQQEGKIFQVPADRWKPIYDQFAAQCKTEHDEVIALGGLHILGTERHEARRI
                     DNQLRGRAGRQGDPGSSRFFLSLEDDLLRVFGGERVKALMYRLGMTEGIPIESKLISR
                     RIENAQMAVEAQNFEARKHLLEYDDVMNKQRETIYSIRRSALEGKEQKDYALGIAEDI
                     GRELVENFCPANEHPEKWNVTQFTAEVLGQYGIDTKVAGIDPTKMNHAELSDTLVDRV
                     KQRYAEKEQLFGEKMMRWLERRIILDIVDTQWKDHLLTLDHLKEGIGLRGYGQKDPLV
                     EFKKEAFTLFEDMMGRIDTETVRYLFLVQPARPEDEAREIERRQRKQQQEMQLQAGAA
                     QAAEQPKPVRTGAKVGRNDPCPCGSGKKYKKCHGVAA"
     gene            5081..7393
                     /locus_tag="VG272_05670"
     CDS             5081..7393
                     /locus_tag="VG272_05670"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HWF13203.1"
                     /translation="MRTALKMVLPLIVSVAVVSVLFAWYQVRTERRILRSDLTRRSQI
                     LGESLQENVEPLVGRAPDKNLQRLVDRFTDREHLMGVAVYDAAGGTLVITSGIAPIFR
                     NWPDIVKDAAHKDQEEDVFVRGKSLDSLGNKNIPPAHIHAVPLHRNDELAGMLALFQD
                     TSYIDAQLSHTLRDSLLNALVQTILITGLALVLVRWTFMGPLKSTAHWLRTLRISGPN
                     GASVPPGLPQGEIFEQLHQEVRHLARDLNTARASAEAEAKLRESGASLWTGERLRVSL
                     RNKLHDTPLFVVSNREPYMHTRNDRDGSINVIVPASGLVTALEPVLIACNGTWVASGS
                     GNEDRGVVDENDRLRVPPDNPSYTLRRVWMTDEEDKGFYEGFSNEGLWPLCHIAHTRP
                     VFRPDDWIQYQRINRRFADVVLAEMEGTESPILLAQDYHFALLPRMVKEARPDARVAI
                     FWHIPWPNPEMIGICPWRRELVDGLLGADLIGFHIQTHCNNFLETVDHAVEALTEWDR
                     FAVNRLGHVTRVRPYPISVAFPETNTFDDEVLNVGTERAAICHELGIEASLLAVGVDR
                     VDYTKGILERFRAIERFLDQYPAFQRRFSLIQIGAPSRTDIVRYKNFLDEVTEEAERI
                     NAKFQAGRWKPIVLLKKHHSHEQIERYYRAASLCLVTSLHDGMNLVAKEFVASRNDNR
                     GVLVLSTFAGAAHELSDALLVNPYDIMQLAEAIHSAIEMPEAEQAKRMQWMRRVVKEH
                     NIYLWAANLLSDLTEIRIEGAERAEVRQTQ"
     gene            7390..8172
                     /gene="otsB"
                     /locus_tag="VG272_05675"
     CDS             7390..8172
                     /gene="otsB"
                     /locus_tag="VG272_05675"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HWF13204.1"
                     /translation="MTRSHRTTPHLFKKWKSIVRRIRGKKQIVIFLDFDGTLVRIAPL
                     PDAVRIQSETKEVLRRLGKLRNVELVIISGRRRADLQRFIGIRNIKYMGLYGWENKNN
                     KKLTYPERVALAQTLVDLLAELPAYPGAWIEAKNNSFSVHLKGLKVETKREVQQVVKK
                     RVGLLSETLQAMTNLRDMEVAPVSIGNKGMAVREVLRQTALRGALPIYFGDDLSDEPA
                     FAAAGKGVSILVGRRRTTRAKFFLRGPAEVTAALSRMEETIR"
     gene            complement(8358..9017)
                     /locus_tag="VG272_05680"
     CDS             complement(8358..9017)
                     /locus_tag="VG272_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13205.1"
                     /translation="MLKLTIRSSAVFVLLLFASVTQAKAQGVSAYFGVGSATDGAATS
                     AGCPSKTVFDEITTQCESAPTIGGAFGLIGADFLILPHLGVNAEYSFRFAQANYLPGA
                     GLNARPSFYDFNAVYQPTTGERRIVPVVEGGIGGSRLSLYFNQASCVTQVCQNSSQVV
                     ATSNHFQLHGAVGVKLYVKSDIFIKPQFDLHWVHNLDQQYGSDFVPQYTIAVGYTFGR
                     H"
BASE COUNT         2091 a         2371 c         2503 g         2221 t
ORIGIN      
        1 attctttgcc cgccgcgtaa tccaattcgc tgacgagtcg catgacccag cccactacag
       61 gcgagatagc agttatatcg gcgcataggc tgacctcaaa gtgtgccgag ggtggctgcc
      121 cgttcgatga actctttgga gtcatacctg gtcttaatcg cgcacggcgc agcggacaga
      181 ttgccgtcga aaaatcaact cagtatccat acgggttaca tcactgcctg actccatttt
      241 ggacccgagt acacctgctt acaagcgatc ggcgaagctc gcgttccgca catgtacccc
      301 gtcttaggca ttaggatcct tcgatgatag gcaggatttc gttgcgaatg tgcggatcta
      361 atttcgcgat tgccgaaatc aattgccata agccggaacg caatgaatga attgtccgaa
      421 ccgaatcgag agcaaagtgg ctgccaatca gttgtgccct ctgttgtcga atcagggact
      481 gtgacgaaaa tgttgtggct attgaggtat acgtccagga cacatacttt gtcattaaat
      541 caagtattga cttgcccgga accaaatcag gaattagcat gtacacgctc ttttacttag
      601 tataaaggta tgtcagctaa gtggttgaca ttggaatttg aggagggtta ccggtatgcg
      661 caagttaaca attctatgcg tggcgctatt ttgcttatcg ctaaccgcgt cagcacagga
      721 ttctccggcg gcgttcgata ccgccagccc tgcaagtgag gccgtgcccg cacccgctcc
      781 agcgccactg cttccgtctg accgcgatcc gtggcaagtc gggttgggat ttcaatactt
      841 gcagtttaat gttttgggcg tgcgctttca tgattttgca tacaaggtgg acggcacacg
      901 atatttgaat aactggtttg gtgtcgaggg agcagtaatc gctggatttg gccatgtgga
      961 aggcgctcca aaagttgatg ccaaatcgct tttcctcggc ggaggaccgc acatttccgt
     1021 ttataacagc cgacaccttg aaccttgggt ccacgcgctg gttggttggg agcacttgcg
     1081 atttactcag ggcgccacgc tgggcgcgga ttcccatgcg gcattttatg caggcggcgg
     1141 agtagattat aaaattagcg ccacgcgatt gttctggcga gtccaggggg actatatcgg
     1201 gacgaatttt ggcccgaaat tgtcaacaaa ttacgcgttt ggcactggtg tggttatcaa
     1261 cttctaattc taaaaccaag caaccatcat tttcttgaat ggccggtatt ttgcgagtac
     1321 gtttcgcttt ataccggctt ttttattttg tgataaacgg ggctgccgga gatctttccg
     1381 tgagaattcc cgatccgcgg aagtgtattg cgatggttcg gctaagcggc cacgccgtgg
     1441 cacttcttgt atttctttcc gcttccacac gggcatggat catttctgcc aactttagcg
     1501 cctgtacgga caggtttggg ctgctcggcg gcttgtgcgg ctccggcctg tagctgcatt
     1561 tcttgttgtt gctttcgttg gcggcgttca atttccctgg cctcatcttc agggcgagcc
     1621 ggctgcacga ggaagagata gcggacggtt tcggtgtcga tgcggcccat catgtcttcg
     1681 aaaagagtga atgcttcctt cttgaattcg acgagcggat ctttttgtcc gtagccgcga
     1741 aggccgatgc cttccttcaa gtgatccagc gtcagcaggt ggtctttcca ctgcgtgtcc
     1801 acgatatcca ggatgatgcg ccgctcgagc caacgcatca ttttctcgcc gaacaactgt
     1861 tccttttcgg cgtagcgttg cttcacgcga tcaacaaggg tatccgacaa ttccgcgtga
     1921 ttcatttttg tcggatcaat cccggcaacc ttggtatcga tgccgtattg gcccaggact
     1981 tccgcggtga attgcgtgac attccatttt tcgggatgct cattcgcggg acagaaattt
     2041 tcaaccagtt cgcggccaat gtcttccgca atgcccagcg cataatcctt ctgctctttt
     2101 ccttccagcg cggagcggcg aatcgaataa atcgtttcgc gctgcttgtt catgacgtcg
     2161 tcatattcaa gcaagtgttt gcgcgcttcg aagttttgcg cttcgacggc catttgcgcg
     2221 ttttcaatgc gacgtgaaat cagcttggac tcgatgggaa tgccctctgt cattccgagg
     2281 cggtacatca gggccttgac gcgctcgccg ccgaacacac gcagcaaatc gtcttccagt
     2341 gaaaggaaaa atctggaaga acccggatcg ccttgacggc cagcgcgccc acgcagctgg
     2401 ttatcgatgc gccgcgcttc gtgacgttcc gtgccaagaa tgtgcaggcc gccgagagcg
     2461 atgacctcat cgtgctcggt tttgcattgc gccgcaaatt gatcgtaaat cggtttccat
     2521 cgatccgcgg gaacctgaaa aattttgccc tcttgctgga acaaaaccat gtttggcgcg
     2581 gtgaccgatt ccgcgccgtc tccgccgatc acggcaggcg ctgaaatgta gggaattgcg
     2641 agcttttcct ggaggcaata ctcgcgcgtc attgcttcag gattcccgcc cagcaaaatg
     2701 tccgtaccgc ggccagccat gttggtagaa accgtgacgg cgcccttgcg tcccgcttgc
     2761 gagacaactt ttgcttcgcg ctcgtgttgc ttggcgttca aaacctgatg cggaatgccg
     2821 gcttttttca gcagatttgc cagatgctcg gatttttcga tggaaatggt gccgaccagt
     2881 accggctggc ccgattcgtg aatttgccgg ataccgtgcg catacgaatt gtcttcctgc
     2941 atgatgccat tgaccaccgc ttcatacttt tccgcggcgg tgcggtacac cacgtccgga
     3001 aattcctcac ggagcagttt gcggttcgtg ggaatcagcg aaacgtccag tttgtaaatc
     3061 ttattgaatt cggtggcttc ggtttccgct gttccggtca tgccggaaag tttcttgtac
     3121 atccggaaat aattctggaa agtgatcgtg gcgagagtct ggttttcgcg ctcgattttg
     3181 acgccttctt tggcttcaac cgcctggtgc aaaccatcgg accagcgccg gcccggcatt
     3241 tcgcgtcccg tgaattcatc gatgatgatg acttcgccgt ttttcaccac atattccaca
     3301 tcgcgcttgt acagcgtatg cgcacgcaag gcctgataca cgtggtgaat ggtttccaga
     3361 tgctgcggat cgtacatgtt gcccaggcca agcaactttt cggttttgct cacgccttct
     3421 tcggtcagcg ttgcggtctt gtgcttttct tcgacgatgt aatcgatgtc ctgaatcagt
     3481 ttcggaatga ttttatcgat gcggtaatac ttgtctgtgg attcttcggc cggaccggaa
     3541 atgatcagag gtgtgcgcgc ttcgtcgatc aggatcgagt caacttcgtc cacaattccg
     3601 aagtggtggc cgcgttgcac gcagtccgcg agatcgaatt tcatgttatc gcgcagatag
     3661 tcaaagccga attcgttgtt cgttccgtag gtgatatccg cgccgtaggc cgcgcgccgt
     3721 tccgcatcgc tcagatcatg aacaatcacg ccaacggtca atccgagtgc gcgataaatc
     3781 ggactcatcc attccgcgtc gcgtcgcgcc aaatagtcgt tcaccgtaac gatgtgtacg
     3841 ccgcgcccgg tgagcgcgtt cagaactgcg ggaagcgtgg ctacgagtgt ttttccttcg
     3901 cccgttttca tttcggcgat cttgccttcg ttcagaacga tgccgccaat cagctgcaca
     3961 tcgaagtggc gcatgccaag ttttcggcga cccacttcgc ggaccaaagc aaacgcgggg
     4021 actagggcgg gcgcgagagc atccttgcaa cgctctttaa agtccggatc ttccagagaa
     4081 atatcgccga gacgcccttg cacctgggtt ttcagttgaa ctattttttc cagaatttca
     4141 gcgtcggtca gcttctcaaa atccggcgag agcgaattga tcgcgttgac atgcggctga
     4201 atcaccttga ggtcgcgctc gtgcttggtg ccgataaacc gggcaacgac ctgatcgatc
     4261 agggtctcga ctcgcatcac aatccttcag tgttcttagg aataaagttc ctgctccaac
     4321 tgcgggcgaa tggcccggcg caagggatgc aggtgcgcgc agcatgaggc catcgtgcag
     4381 cgacactcaa aagttcattg taccctgtca ggcaatcgcc gtgcaaagag aggtgtgagc
     4441 attgcccctc agagataagg acttccttcg ttttgtggag cagaattcct gcaagacgca
     4501 agtcgctccg tctcacattt gccatgtata ggtagtatat tgattacatt gaacttctct
     4561 tggataatcg ctttcttcgg tcacccttcc ggagagcaaa ctatctatct ccccgcctac
     4621 tttatcaacg caatatctga cggcaaattt gggattttac ctacagcaat ccggggcaaa
     4681 aaataatgca tctaccttac ggacgacacg agcccgattc gtgcagtatg caggaccagt
     4741 gctaaaagtt ttcctataat catttacgga aagagagcaa tcggaaaaaa ttgcatagcc
     4801 gcgggaggcc aaaaccaagc gcacttccat gaaatatcca gtgcgacgcc cgcaagtcgt
     4861 tgagcccact aaggctgtag cgataattga aaatctttaa cgtttggcgc tcatagtgcc
     4921 tccatatgcc cctatccgga atcagcttat cattgtccat ggaggagcta acccaaaaaa
     4981 ctaacgaagc aaggaccctt cgaaggatgt tttttattca ggtcgcccgg aaccccgccc
     5041 ggtgcgacct attctcttga cagataatcg aggaatatcc atgcgcacgg ctctgaaaat
     5101 ggtcctgccc cttatcgtga gcgtagcagt ggtgtccgtg cttttcgcct ggtatcaggt
     5161 ccggaccgaa agacgcatct tgaggagtga tctgacccgc agatcgcaaa tcctggggga
     5221 aagtctgcag gaaaatgtgg agccattagt gggccgcgct cccgacaaga atttgcagcg
     5281 attggtggat cggtttacgg atcgagagca tttgatgggg gtcgcggtgt acgacgcggc
     5341 tggcggaaca ctggtcatca cgtctggaat tgcgccaatc ttcagaaatt ggccagacat
     5401 tgtaaaagac gcggctcata aggatcagga agaggatgta ttcgtacgcg gaaaaagttt
     5461 ggacagttta ggaaacaaga atattccgcc cgcacacatt cacgcagtgc cgctgcacag
     5521 gaatgacgaa ttggcgggca tgctggcgct ctttcaggac acgagctata tcgatgccca
     5581 actttcgcac acgcttcgcg actctttgtt aaacgcgctg gtccagacaa tcctgatcac
     5641 gggattggcg ctggttcttg tcagatggac cttcatggga cctctcaaga gcactgccca
     5701 ttggctgcgc acactgcgca tcagcggccc gaatggagca agcgttcctc ccggattgcc
     5761 gcagggagaa atttttgagc agctgcacca ggaggtcagg cacctcgcgc gcgatttgaa
     5821 cacggcgcgt gcctcggccg aagcagaggc gaagttgcgc gagtccggcg cttctctgtg
     5881 gacgggcgag cggctgcgag tgagcctgcg caataaactt cacgatacgc cgctatttgt
     5941 tgtctccaac cgcgagccat acatgcacac acgaaacgat cgagatggat cgatcaacgt
     6001 cattgttccg gcgagcggac tcgtgacggc tctcgagcct gtgctgattg cgtgcaacgg
     6061 gacatgggtg gccagcgggt caggaaacga agatcgtggc gttgtggatg aaaacgatcg
     6121 gttgcgtgtg ccgccggata atccgagtta cacgctgcgg cgcgtgtgga tgacggacga
     6181 agaagacaaa ggtttttacg aaggcttctc gaacgaagga ctttggcctc tttgccatat
     6241 cgctcatacg cgtccggtgt tccgacccga tgactggatc caatatcagc ggatcaacag
     6301 gcgatttgcg gatgtagtgc tggcggaaat ggagggcacc gaatcgccca ttcttctggc
     6361 acaggattat cacttcgcgc ttctgccacg aatggtcaag gaagcgcggc cggacgcgcg
     6421 cgtggcaatc ttctggcaca tcccgtggcc gaatccggaa atgattggca tctgcccgtg
     6481 gcgaagggaa ctggtcgatg gtttgctcgg cgcggacttg attggtttcc atattcaaac
     6541 gcactgcaat aattttcttg aaacagtcga ccacgccgta gaagcgttga cggagtggga
     6601 tcgcttcgct gtaaaccggc tgggacatgt gactcgcgtg cggccatatc ccatcagcgt
     6661 cgcgtttccg gaaaccaaca ctttcgatga tgaagtcctg aacgtgggga cggagcgggc
     6721 cgcaatttgt catgaattgg gaattgaagc ttcgttgctg gccgtgggcg tggatcgcgt
     6781 ggattacacc aaaggaattt tggagcggtt ccgggcgatt gaacgctttc tggatcagta
     6841 tcccgcgttc cagcgcagat tttccctcat ccaaattggg gcgccaagcc gcacggatat
     6901 cgttcgctac aagaattttc tggatgaggt caccgaggaa gcggaacgaa tcaatgcgaa
     6961 gtttcaagcg gggcggtgga aaccgattgt tcttctgaaa aaacaccact cgcatgaaca
     7021 aattgagcgc tactaccgcg cagcctcctt gtgcctcgtt acgtctctgc atgacggaat
     7081 gaatttggtg gccaaggaat ttgtggcctc gcgcaacgat aaccgcggcg ttctcgtcct
     7141 cagcacgttc gccggtgcag cccatgaact ttccgacgct ttgcttgtca atccgtatga
     7201 catcatgcaa ctggcagaag ccattcactc cgcgatcgaa atgcccgagg cggagcaagc
     7261 caagagaatg cagtggatgc gccgcgtggt gaaggaacac aatatttatc tatgggcggc
     7321 aaatttgctc agtgacctga cggaaatccg gatcgaaggt gctgaacgcg cggaggtgcg
     7381 gcagacgcaa tgacccggtc gcatcgcacc acaccacatt tgttcaaaaa atggaaaagc
     7441 attgtccgac gaattcgcgg aaagaaacaa atcgtgattt tcctcgattt tgacggaaca
     7501 ctcgtgcgca tcgccccatt gccggacgcg gttcgcatcc aaagcgaaac aaaagaagtg
     7561 ctgcggaggc tgggaaaact ccggaatgtg gaactggtga tcatcagcgg aaggcgacgt
     7621 gccgacctcc agcgatttat tggcattcgc aatattaaat acatgggcct gtatggttgg
     7681 gaaaataaaa acaataaaaa gctgacatat ccggagcgtg tggcgctggc acaaacgctt
     7741 gtggatttgc ttgcggaact tccagcctat cccggcgcgt ggatcgaagc gaagaacaat
     7801 agtttttcgg ttcacttaaa gggcctgaag gttgaaacca agcgtgaagt ccagcaagta
     7861 gtgaagaagc gcgtgggatt gttgtccgaa actttgcagg ctatgaccaa tctccgtgac
     7921 atggaagtgg cgcctgtttc cattggcaat aagggaatgg cggtgcgaga agttttacgc
     7981 caaaccgcgc tgcgcggagc gctgccgatt tattttggcg atgatttgtc ggatgagccg
     8041 gcctttgccg cggcagggaa gggggtttcg attttagtgg gcaggcggcg gacaacgcgg
     8101 gccaagtttt tcctgcgagg gcccgcggaa gtgacagcag cactttccag aatggaagaa
     8161 acgattcgat gacagtggtg gaaacgccat ttgatttttt gcagcgggga atttaaaacg
     8221 ctgagagatt catcgaaaaa gaagtgaagt cgcgaggcct cgcgattgtt gcagtgagtt
     8281 aaaaaaacat gcgcgccgtc tgatgaagtc aggcggcgcg caatctttcc acagtctctg
     8341 tagagtgcag cgattcttta gtgccgtccg aaggtgtaac ccacggcgat cgtgtactgc
     8401 ggcacaaagt cgcttccata ttgctgatcc aaattgtgca cccagtgcag atcgaattgc
     8461 ggcttgataa atatatctga cttgacgtac agtttcacgc ccaccgcacc gtgcaattgg
     8521 aagtgattgg aagtcgctac aacttgactc gaattctggc aaacctgcgt gacgcagctc
     8581 gcctggttga aataaagtga caacctcgat ccgccaattc cgccctccac cactggaaca
     8641 attcggcgct cgccggtcgt gggctgataa accgcattga agtcataaaa ggaaggtcgc
     8701 gcatttaagc cagcgccggg caaataatta gcctgagcaa agcggaagga gtactcggcg
     8761 ttaactccga gatgtggcaa gatcaggaaa tccgctccaa tcaggccgaa cgcgccgccg
     8821 attgtcggag cagattcgca ttgtgtggtg atttcatcga agactgtttt ggaaggacag
     8881 cccgcactcg tcgcagcgcc atcggtcgcg ctgcccactc caaaataggc gctgactccc
     8941 tgggctttgg cctgagtaac gcttgcgaac aagagaagaa cgaataccgc gctcgatcgg
     9001 atcgtgagct ttaacaatga aaatctcctt taattgaagg ttcgaatgcc taagaaactg
     9061 gtattgaaaa aatgcgcatc ctggccggcg taaaaccttc cccctcgcaa gccaaaatgc
     9121 gataggaagc ttcaacctta tcttcccatt acctagatgt acaacaccgg tttgttcagc
     9181 aattcc
//
LOCUS       DASZMB010000125        19903 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3428554, whole genome shotgun sequence.
ACCESSION   DASZMB010000125 DASZMB010000000
VERSION     DASZMB010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 19903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19903
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3428554"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1251)
                     /locus_tag="VG272_05685"
     CDS             complement(148..1251)
                     /locus_tag="VG272_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWF13206.1"
                     /translation="MIIPEELSARISASNLARFALALFVTLLALLIGRATNALASDTP
                     GYCMAFMAVALSTWLFGTGPATASLALSLLTVNYWLVPQTPPLRITPTAGWISFALFL
                     LAAILIIVIGETNLRKQERLRNAAGLLEEKVRERTAELDHANYSLRQLTGRLLNLQDE
                     ERRRIARELHDNAGQALSVLAMNLGAVAEDLGRLMKTAGTVADSAGMVRQMSDDIRTM
                     SYLLHPPLLDEMGLAPALKWYVEGFAERSKIAVDLQCSSEIGRLPREVETAIFRIVQE
                     CLINIHRHSGSPTAAIQIDSEDDFVRLEVRDNGTGIPPELRRQMETGGTIGVGLRGMR
                     ERASQLGGSLEISADGTRQGTCIMVRLPAAEAV"
     gene            1575..2702
                     /locus_tag="VG272_05690"
     CDS             1575..2702
                     /locus_tag="VG272_05690"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HWF13207.1"
                     /translation="MKTTIRNFVFLLLIIALFSPAILADTISLKNGDRLTGTIVKSDA
                     KTLVLKTEFAGDVTLQWAAIDTINSTQPLHVGLAGGQMVDGPVATKDGQIQVTTTSAG
                     VVSASKDSVQVIRSDAEQAAYDATLERLRHPHLGDFWSGTLDTGLSLTRGNSSTLSYT
                     LAGRATRLTDRDKITVYTTAVYGKTSVPPPAQVTAHQITGGVRADINLSPRWFVFAAT
                     DFNSNALQHLDLQNVVDGGAGRHLINTKNTQFNVYAGVGYNQEFFGAFLAANPTPPPA
                     TITVAAVTQRNAEANLGEEFDSKLGSRSTFSETFNYFPNISGPSGYRYTFNTVLSTAI
                     SKWLGWQFSLADNFISNPPDGIKGNDLLLSTGLRLVFGNPK"
     gene            complement(2750..3313)
                     /locus_tag="VG272_05695"
     CDS             complement(2750..3313)
                     /locus_tag="VG272_05695"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HWF13208.1"
                     /translation="MAAQKRNRVLLFGGLGIAIIFLGIFALRTYGQFQHSKYTSPPAK
                     VGATIGGKKITIEYYAPSMHGRKVMGGLVPFGEVWCTGANWATKITTEAPLQMGSLKL
                     PAGSYSIWTIPNANEWTLIINKRTGQFHLDYDSSTDFGRTKMNLKTLPAPVETFKINL
                     KDDGGNQGTLALDWETTEASIPLTVLP"
     gene            complement(3326..4960)
                     /locus_tag="VG272_05700"
     CDS             complement(3326..4960)
                     /locus_tag="VG272_05700"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HWF13209.1"
                     /translation="MYRIQKLLGLLGATIFLAALAHGATITGTVKGPDGAPFKGAFVS
                     AQNTKTKITMNVLSHKDGAYRVDDLPAGEYVLRIRAVGFQADPRTGVALTANQSASMD
                     WTLKTGPVRWRDLSLYQGEKLIPNIRGKEMVFGPLSNFRDAPCQICHGFQTRMAAISR
                     DSDGAKDRVDYMRTRMHFLLGSMVNDEQADKVASFISTAFGPESVLPKSPADLAEYKN
                     TVRTFDDDAMNIVYVEYDLPGPNRMPWSAAPDKEGNIWMPYYGGANRIGKLDPETGNV
                     KEYDVPNENAVAIHSAYPAADGSIWLAEFGLNRLGKFDPKTERLSEFADTYTPGKEGL
                     VAGGSKHTVRVGPDGVAWASGTRVSRYDPKTGKFMDFNEPTYGVVVDKDGNCWFSEYN
                     AVGKIGKIDGKTLEVKTWNPPGSNTVYSRRIQIDSDGMVWFSQSGAGQIGRFDPKTET
                     FKNYALPGPNASPYAMNLDRNHDVWYSSEYLDEVGRLNTTTGKVTEYPFPHSEITSRE
                     YFLDAQGRMWYATPSNNKVGYFYVAGQGTMARNGGQ"
     gene            5128..5613
                     /locus_tag="VG272_05705"
     CDS             5128..5613
                     /locus_tag="VG272_05705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HWF13210.1"
                     /translation="MKKNPKTIGVISDTHGLLRPQALRALEGSDLIIHAGDVGDPKIL
                     DALKTLAPVFAVRGNVDTEKWAQALPENEVIETGPATIYVLHDVHALDLNPFAAGFHV
                     VVSGHSHKPEQTERDGVLFLNPGSAGPRRFDLPVTVARLDLTKKPWEVTFINLLKIEI
                     A"
     gene            complement(6048..7277)
                     /locus_tag="VG272_05710"
     CDS             complement(6048..7277)
                     /locus_tag="VG272_05710"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWF13211.1"
                     /translation="MQNFTELQISNYIKERLAFAKFATPTPVQAAAIPVALDGKDVLA
                     TAQTGTGKTLAFLIPIFERLLAQKTKGIEALVLVPTRELAMQVVDQYNALRGKQLPPA
                     ALVVGGLPEAKQLAAIRAGARLIVATPGRLEDYLGRNLFNFKTLHILVLDEADRMLDM
                     GFLPAIRRIAAVLPKDRQTLCFSATLEASVANLVKDYMRSPVRLAFGNILKPAENVRV
                     QAFEVSGDRKHEVLQHLLGKETGRCLIFARTKRGTDKLARNLIREGYAAAVIHGDRSQ
                     SQRTAALTGFQQGRYRILVATDVAARGIHVQDIAHVINYDLPDIAENFIHRVGRTGRA
                     GKSGVASTLYGREQRSEMLHLERTLGIRMERMQADGTSIPESKPRAKAETPRFNTAPV
                     SGMVRLPGEVLQAQLEN"
     gene            complement(7601..8332)
                     /locus_tag="VG272_05715"
     CDS             complement(7601..8332)
                     /locus_tag="VG272_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13212.1"
                     /translation="MRHKALGRYVVLISILSIMGCNKSTTSTDSTNSPANGDAGSSAT
                     ASSSNPGTASNASGQKNSESSIGHIFAPKPIVISTGTSIEVTVDQLVSSKDSNSGDHF
                     DASLAEPVVVGDKVILPKGTRAVGTVTSAKSAGRFKGNAAISVTLTSVTLNGKKYDVN
                     VSEITQSGKGRGKRTAEGAGGGAIVGGLIGALAGGGKGAAIGAGAGAGAGTAGTALTG
                     DRDVTIRPETRLRFKLMEPLEVRKS"
     gene            8546..8872
                     /locus_tag="VG272_05720"
     CDS             8546..8872
                     /locus_tag="VG272_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13213.1"
                     /translation="MAIEKTITLMPAEGPAPMKRHSRAQMAIAFGIAAVSDVFSIWLT
                     FAPPFQWTLDIATAIALFLILGKRWALLPGLIAEAIPGMGVFPVWVLVVLSVIVYDGI
                     KKPVRA"
     gene            9070..10203
                     /locus_tag="VG272_05725"
     CDS             9070..10203
                     /locus_tag="VG272_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWF13214.1"
                     /translation="MNKFSNSFSRRNFLAGASSLGAFYAASKFALPLPALAAPLIDDP
                     RIGPQPIADKGFASVRKIGNGLYATIADRSKGLQARCNGGFLIGRDAAIMLEGFQTTL
                     GSAFQLETLRSVTQIPIRAAINTHWHFDHTLGNSVYGGTGIPIWAHADVPARIATYYP
                     KWQAQDLNTYLVPWQKRVADAKSEMQREHTKSDIEGLTGMFVPVSQTVLALPNHLLDP
                     AKMPMEIDLGGLTVVVEHYVGHTDTDLIFRVPDQNIVYTGDLLTGGQYPVNINGYPSK
                     WRTTLSKFATFDKNTLFVPGHGQIMGQEGVGEMRSCFDDIAEQAEKSYKAGVPVEDAA
                     ERYVVPEKFKNYRQFSWGFCITRTYEQFYAEWSGKPVKILNYS"
     gene            10662..11048
                     /locus_tag="VG272_05730"
     CDS             10662..11048
                     /locus_tag="VG272_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13215.1"
                     /translation="MIAVSDKYALEVASPTPIASARQHEEYLSVLDKLTNKQNATREE
                     EKYAEVLITLIETYEEEHHSIPDASPIEILRSLMDANDLRQKDMISIFGSESIVSEVL
                     HKKRGLNKTHIEKLSKRFRVSPALFF"
     gene            complement(11139..12638)
                     /locus_tag="VG272_05735"
     CDS             complement(11139..12638)
                     /locus_tag="VG272_05735"
                     /inference="COORDINATES: protein motif:HMM:NF005686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWF13216.1"
                     /translation="MSENEPTRREILLGTAAALLLKNAPEAHATADSICFMSVVEMAR
                     LIREKKLSAREALAAHLKQIERVNPNVNAIVTFVADMATDAAAKADEMQAHHEKLGPL
                     HGLPVAHKDLLETRGIRTTFGSPLYKDYVPKEDDIVVERMSRAGAITIGKTNVPEFGA
                     GSQTFNKIFGATRNPYDLTKTCGGSSGGAAVSLACGFVPVASGTDTGGSLRNPAAFCN
                     VVGFRPSVGRVPNPKAAFAWSTLSTSGCLGRSVADLAYVLSTIAGPDSRAPLSINEPG
                     EIFARPLERNFKGVRVAWFKDLGGLPFDPRVRAVIDGQRKTFESLGCIVGQAEPDFAP
                     AEIAFRILRALTSANTYGARLHDHPDAFKDTLKGEIEEGLHLTGMEVAQAETAHGQLW
                     RGFQAFLEKYEYFILPTTQLPPFDVDSPYPTEIAGVKFSNYIDWMKSCWYISATSNPA
                     ASVPAGFTTEGLPVGIQIVGRAKEDFSVLQLAHAFEQVTGFGKKRPSIV"
     gene            complement(12871..15072)
                     /locus_tag="VG272_05740"
     CDS             complement(12871..15072)
                     /locus_tag="VG272_05740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HWF13217.1"
                     /translation="MAEKPWSSTAPPAEFRPYVEASSAMTEFSWRAVILGSLFGILFG
                     AVSVYVGLRAGLTVSASIPISVLSISILRAFGKSTILENNIVQTTGSAGESVAAGVIF
                     TLPALIFLGFSLNTEYWRVFFLALIGGWLGVLLMIPLRRQLIVKEHGNLTFPEGTACA
                     DVLVAGERGGSFAGRVFWGLGLGGVYTFCMNTLGMWPGQPEYRPTWLPGASFRAAVTS
                     EYLGVGYIIGPRVSGILFAGGVISWLVLMPAIKFFGSLSPTVPLYPSTIPIPQMTPDQ
                     LWASYIRPMGAGAVAASGLITLIKTMPTIFSALTAGLNDMRKAREGAAESDRTERDLS
                     MRLVFIGSTVLLAMMWALLRFKPIPGAQTGAFANLIAAIFVIVFGFLFVTVASRISGL
                     IGNSSNPISGMTIATLMATCAVFLVIHWTANAYAVLALTIGGVVCIAAAIAGATSQDL
                     KTGYLVGATPYRQQIALVIGVLVSSFAIGGTLILMNIGLAQYKPMQIPLDINSLPTGV
                     ERQDNAFQHEGKTYVLLNAIGSAEIPDGKYLFDPVTNRIEVQWVQGIGSDQAAAPQAR
                     LMATVISGILNQRLPWRLVFLGVFLVIAVELLGVRSLPFAVGSYISIATTMAMFAGGL
                     LRWIVERGTEQKSEADSEVSPGSLYSSGLIAAGGVFGLLAIFLNLLQDPELSKRLPIW
                     LTHMFFLPWPAKIFAFGERFMPQASQSPWVGVVMFVLLASSLFIFARRKLE"
     gene            complement(15410..16018)
                     /locus_tag="VG272_05745"
     CDS             complement(15410..16018)
                     /locus_tag="VG272_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13218.1"
                     /translation="MPLPETISVRYTEEEAGYVTVRPLVRQTFSLDELLDMILSVAGK
                     DTTRVQQLLHSGTVVYHFYRYTWTGFDADESELAAALAKFPDTDPSRAFSPNLCTNAV
                     FDGPGVNPRHLLDLMKDAASSRRIFRRQSFWQRLMDIASEENLVYEKYSYSRRADLYR
                     LIIDSENVLKIAEAADQLARRNLRPALRVVNATACIFFVCPR"
     gene            complement(16137..16592)
                     /locus_tag="VG272_05750"
     CDS             complement(16137..16592)
                     /locus_tag="VG272_05750"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HWF13219.1"
                     /translation="MALMERIQKDLTAAMMSKDELRLSVLRMVKSALKYKEIEKVRPL
                     EDAEALQVLQTLMKQRRESVDQFLKGGRQDLVDKETKEIAILEDYLPAGASDADMNEA
                     IEAAILETGANSPKSMGAVIKAAKVRLEGKTVDGKTLSDRVRDRLSKMA"
     gene            complement(16868..17056)
                     /locus_tag="VG272_05755"
     CDS             complement(16868..17056)
                     /locus_tag="VG272_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13220.1"
                     /translation="MAMAKESNLDKPDPASEEEDKGTLVAIEEGIRDADAGRTTPIEE
                     VRKLMHTWITASSSRKER"
     gene            17180..18724
                     /gene="der"
                     /locus_tag="VG272_05760"
     CDS             17180..18724
                     /gene="der"
                     /locus_tag="VG272_05760"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HWF13221.1"
                     /translation="MTEKLPSVVIVGRPNVGKSTIFNRLTGTRRSIVTDEPGITRDRI
                     YGTANWQGKSFEVVDTGGIVPDDKAAIPREILHQAQVAIESALQLLLVVDARAGLTPL
                     DAELARLLRRTGKPLAILANKVDTQMQESLAAPFYELSKEVFPVSAEHGYGFDNLLDE
                     MTSRLGEAKPQEAESSASEKQINVAIIGRPNVGKSTLLNRLAGVERAIVSDIAGTTRD
                     AVDTVVEREGHTYRFVDTAGIRRRGKTNLVAEKMSVMMARRHLEQADVALLIVDASLG
                     VTANDANIAGYAEESGRSVIIVMNKWDLALEAAAEKAERELEHEGRKPHVAVARTRKH
                     TSVVRKKAVTGKVDPKQLAEDYEKIVRTRFKFLDYAPIVFVSAKTGDHVEKLFALINK
                     LADARRRRITTGELNRWVSEVDLDRGTSPAARRVKIYYVTQAGTSPPTFILFTNQSKR
                     LHFSYERFLENLLRKSFDFTGTPIRFLQRMKERRDWTKPTSRRTPEPKHPGMSHNRVK
                     RAPPAD"
     gene            18916..19419
                     /locus_tag="VG272_05765"
     CDS             18916..19419
                     /locus_tag="VG272_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWF13222.1"
                     /translation="MEEIEDNGNANAHRHVSDGGDLEPGIPGKNLFRIGEVSRLTATK
                     PFVLRYWETEFPMLQPVKSPKGHRLYRQEDIDTVFTIKRLLYNEGFTIAGARRHLRDQ
                     ALGTSGGVGTNGGAVSVAAEHETQGNESASQAAPALSPHLAVFNRQTLLDLRDSLRSL
                     LTALESE"
     gene            19548..19624
                     /locus_tag="VG272_05770"
     tRNA            19548..19624
                     /locus_tag="VG272_05770"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19582..19584,aa:Pro,seq:ggg)
BASE COUNT         4597 a         5455 c         5359 g         4492 t
ORIGIN      
        1 atagagatac gacgggatat ccaatggtcc ctgaccaaga gggagaagga taggcgacaa
       61 cacccaaaca agcattgcga accagccggg tatgtattgg gacagaactt cggctggccg
      121 attagatgac tttgtcgcgc gccgtgtcta aacagcttct gcggctggca accgtaccat
      181 gatgcaagtt ccctgccgtg ttccgtcggc gctgatttcg agactgccgc ccaattggct
      241 tgccctttcc cgcattcctc gaaggcccac tccaatcgtg ccgccggttt ccatctgtcg
      301 acgcaactcg ggtgggatcc ctgtgccatt gtctctcact tcgagtcgta caaaatcgtc
      361 ctccgaatcg atctgaatcg cggcggtcgg gctgcccgaa tgacgatgaa tattgatcaa
      421 acattcttgc acgattcgaa agatagctgt ctccacttct cggggaagcc gcccaatctc
      481 gctgctgcac tgcaagtcga ccgcaatctt acttcgttct gcgaatcctt cgacatacca
      541 cttgagggcg ggagcaagtc ccatttcatc aaggagcggc gggtgaagca ggtaggacat
      601 cgttcgaata tcgtcgctca tctgccggac catgccggca ctgtccgcca cggtgcccgc
      661 ggttttcata aggcgtccga ggtcttctgc gacggcaccg agattcatcg ccaggacgga
      721 gagtgcttgc ccagcgttgt cgtgcaattc ccgtgcgata cgccttcttt cctcgtcctg
      781 caaattcagc agacgcccgg tgagctgtcg caggctgtaa ttcgcgtgat cgagttcagc
      841 ggtccgctcc cgaacttttt cttcgagcag tcctgcggcg ttccgcaagc gttcctgttt
      901 gcggagattt gtctccccga tcacaatgat caaaatcgct gcaagcagga acaaagcaaa
      961 actgatccag cctgctgttg gtgtgattcg cagcggaggc gtctgaggga caagccagta
     1021 gtttaccgtc agcagtgaca acgcaaggct cgccgtggcg ggcccagttc cgaacaacca
     1081 ggtagataat gccacggcca tgaacgccat gcagtatccc ggggtgtcac tagccaaagc
     1141 gttcgtcgca cgaccgatga gcaaagctag gagtgttaca aagagtgcga gggcgaagcg
     1201 agccagattg ctggcggaaa ttcttgcgga aagctcttct ggaataatca tgccgttcct
     1261 tctcactcac ttacggaacg ggctggtgga ccgcagtcac aaattctaat tcgcgataga
     1321 actcagcgtc aatatgcaag tcacaagctc gcaagacaga cggatcaagg cgaacgccct
     1381 tttgagggtt ggagcattga aaatgcaatg gagcacggta tacattaatc tcagtcgggt
     1441 aaaggcatcc ggatttcccg gctcgccaag aagtcgtatg ttgagttaat cttttttttg
     1501 ttacaccgcc gcgcccacgg ccaaaatgcc tgccattttg ctgaaccaaa tcatagagaa
     1561 ggagtgttgt ttccttgaaa accacgatac gcaactttgt attcttgctg ctgatcatcg
     1621 ccctgttctc ccccgcgatt ctcgcagaca ccatttccct gaaaaacggc gaccgcctca
     1681 cgggcacgat tgtgaaatct gacgcgaaga cgctcgtact taaaacagag tttgccggcg
     1741 acgtaacact ccaatgggca gccatcgata caatcaattc cactcaacct ctgcatgtcg
     1801 ggctggcggg cggccaaatg gtcgatgggc cggttgccac gaaagatggg cagattcagg
     1861 tcactaccac atccgcgggt gtcgtgtctg cctccaaaga ttccgttcag gtgattcgat
     1921 ccgatgccga acaggccgca tacgatgcca cactcgaacg gctgaggcac ccgcacctcg
     1981 gcgacttctg gagtggaaca ttggatacgg gactgagcct tactcgaggg aattcgtcca
     2041 cgctttccta tacacttgcg ggacgggcca ctcgtttgac ggaccgcgac aagatcaccg
     2101 tctatacgac tgcggtgtat ggaaagacga gcgtaccgcc gccggctcag gttactgccc
     2161 accagataac tggtggcgtc cgcgccgata tcaacttgtc tcccagatgg tttgtttttg
     2221 ccgcgacgga tttcaactcc aacgcgctcc agcatctgga tctgcaaaac gtggtggatg
     2281 gaggcgcagg tcgccacctc attaacacca aaaacactca attcaacgtc tatgcaggtg
     2341 tggggtacaa tcaggaattt tttggagcgt ttctcgcggc gaatccgaca ccacccccgg
     2401 ctacaatcac cgttgccgcc gtcacacaaa gaaacgcgga agccaacctc ggcgaagagt
     2461 ttgattccaa gcttggcagc cgatccacgt tcagtgagac atttaattat tttccgaata
     2521 tcagcgggcc cagcggctac cggtacacat tcaatactgt actctccacc gcgatcagca
     2581 aatggctcgg ctggcaattt agtctggctg acaatttcat cagcaatccg ccggacggaa
     2641 tcaaagggaa tgacttgctg ctttccacag gcttgcggct tgtattcggg aacccaaaat
     2701 aatccgcggt cgtagtgcgt gatcgagtgg ctgcatcaag aggaaaaaat taaggtaaca
     2761 ccgtcagagg aatcgacgct tccgtcgttt cccaatcgag tgctagcgtt ccctgatttc
     2821 cgccgtcatc ctttaaatta attttgaagg tttcgacggg agcagggagc gtttttaaat
     2881 tcatcttcgt acggccaaaa tccgtggatg aatcgtagtc caagtggaat tgccccgtcc
     2941 gcttgttaat aattaatgtc cattcgttcg cgttcggaat cgtccagatg ctgtagcttc
     3001 cggcgggaag ttttaaactg cccatctgca agggcgcttc ggtcgtaatc ttcgtggccc
     3061 agtttgcgcc ggtgcaccag acttccccga acggaacgag tcctcccatg actttgcgcc
     3121 catgcatgga cggcgcgtaa tattcgatcg taattttctt gccgccgatg gtggccccaa
     3181 ccttggctgg agggctggtg tacttcgagt gctgaaattg gccgtaagtc ctcagcgcga
     3241 aaatgcccag gaaaataatt gcgattccta agccgccaaa taataaaact cggtttcttt
     3301 tttgagctgc catgctgtgc ttctcctact ggccgccgtt cctggccatc gtgccctgcc
     3361 cggccacata aaaatatccc actttgttgt tggatggcgt tgcataccac atgcgaccct
     3421 gcgcatcgag aaaatattcg cgcgaagtaa tttcggaatg tggaaacgga tattccgtga
     3481 ccttgcctgt ggtcgtattc aggcgtccca cttcatccag atattccgac gaataccaga
     3541 catcatggtt gcggtcgaga ttcatcgcgt acggactggc attcggtccc ggcaaagcat
     3601 aattcttgaa cgtttctgtc ttgggatcga atcgtccaat ttgtcccgcg ccggattgac
     3661 tgaaccagac cattccatcc gaatcgattt gaattctacg cgagtacacc gtattgcttc
     3721 cgggcggatt ccaggttttt acttcgagag tttttccgtc aatttttcca attttcccaa
     3781 ccgcgttgta ttccgagaac cagcagttgc cgtccttgtc caccactacg ccataagtcg
     3841 gctcgttgaa gtccatgaac ttccctgttt taggatcgta gcggctcacg cgtgtcccac
     3901 tggcccacgc tacaccgtcg ggaccgacgc ggacagtgtg cttgcttcct cccgcgacca
     3961 acccttcttt gcccggagtg taggtatcgg caaattctga aagtctctcc gtcttcggat
     4021 cgaattttcc cagtctgttg agaccgaatt cggcgagcca gatactgcca tccgcggcgg
     4081 gatacgcgga gtgaatggct accgcgtttt cattgggcac atcgtactct ttcacatttc
     4141 cggtttccgg atcgagcttt ccaatcctgt tggcgccgcc gtaatacggc atccagatgt
     4201 tgccttcttt gtcaggcgcc gcgctccacg gcatgcgatt cgggccgggc aaatcgtatt
     4261 cgacgtaaac aatgttcatc gcatcgtcgt cgaatgtgcg tactgtattt ttgtactccg
     4321 ccaaatccgc cggcgattta ggcagcacgg attcggggcc gaaagcagtc gagatgaacg
     4381 acgccacttt gtccgcctgc tcatcgttca ccatgcttcc cagcaaaaaa tgcattcgcg
     4441 tgcgcatgta atccacgcgg tctttcgctc cgtccgagtc gcgcgagatc gcggccatgc
     4501 gtgtttggaa tccatggcag atctggcagg gcgcatcgcg aaaattggac aagggaccga
     4561 ataccatttc tttgccacga atattcggaa ttaatttttc gccctgataa agagaaagat
     4621 cgcgccatcg cacgggccct gttttcaaag tccaatccat ggatgcggat tgatttgcgg
     4681 tcagcgcaac gcccgtgcgc ggatcagctt gaaacccgac agcacggata cgaagcacat
     4741 attcacctgc gggcaaatca tccactcgat aagcgccatc cttgtgcgac agcacattca
     4801 tcgtaatctt ggtcttggta ttttgcgccg acacaaacgc gcccttgaat ggcgcgccgt
     4861 ccggaccttt caccgtcccg gtgatcgtcg cgccgtgcgc cagagcagca agaaaaatcg
     4921 tggcacccaa caagcctaaa agtttttgga ttctatacat ggcggctcct tgccggcgta
     4981 acgcgcttca caaatcccaa atggagtcat gcaaaattcg gctcccaacc caggcagcct
     5041 ttattgaggg aatcgtactc cagaattggc cgcccaagcc agccttgttc gtggccattc
     5101 ccaggagcgc cctgtaaact tgcgcccatg aagaaaaatc caaaaacgat tggcgtaatc
     5161 tcagatacac atggcctgct gcgtccgcag gcgctgcgcg cactcgaagg ctcggatctg
     5221 atcattcatg cgggtgacgt aggcgatccg aaaattcttg atgcactaaa aacgctggct
     5281 ccagtttttg cagtccgggg aaatgtggac acagagaagt gggcgcaagc cttgcccgaa
     5341 aatgaagtta tcgaaacagg cccagcgacg atctacgttc tgcacgacgt tcacgcactc
     5401 gatctcaatc cattcgccgc gggatttcat gttgtggtga gcgggcattc gcacaagccg
     5461 gaacaaacgg aacgcgacgg tgtcctgttc cttaatccgg gaagcgccgg tccgagacga
     5521 ttcgatttac cggtgacagt cgcgcggctt gatctcacaa agaaaccctg ggaagttaca
     5581 tttatcaact tgctgaaaat tgaaatcgct tgactcgcat tttttcgtct ggacttcggc
     5641 ccgataaggg atgtcatatc gcgggaaagt tcggcgagtt ttcggtggcg ccggcggctt
     5701 tgcgcatcca catcacaatt caaaaccgcc ggcgagacgc cggcgctacc aggagcgcga
     5761 ttctattcgt actgagaact gcagcaatgg caatacgtcc ggaagttaag tatcagcgcg
     5821 cttcgttttt catttcaaac gtggcgatcc ggggcaaccc aacttgcgaa cgacgctcaa
     5881 cttgatcaat acgttccagc gacgcgaata gcgccccgga gtaggggcac gatatatcgt
     5941 gcccctactc tggcagggct ggcccaagca ttgaatatat ccgcatagaa taaaaaacgc
     6001 cggaagcctg cattaaattg caggcttccg gcgtgagttg tgtgcagtta gttttccagc
     6061 tgagcttgca gcacttcgcc gggaaggcga accatccctg aaacaggcgc ggtattaaag
     6121 cgcggtgttt cggctttggc tcgtggcttg ctttccggga tgctggtgcc gtccgcttgc
     6181 atccgctcca tgcgaatgcc gagcgtgcgc tccagatgaa gcatctcgga ccgctgttcg
     6241 cgcccataaa gtgtcgaagc gacgccgctc ttgcccgcgc ggccggtacg gccaacacgg
     6301 tggatgaagt tttccgcgat atccggcagg tcgtaattga tgacgtgtgc gatatcctgc
     6361 acgtgaatgc cgcgtgccgc tacatccgtg gccacaagga tgcgatagcg tccctgttga
     6421 aatccggtaa gagctgcggt tcgctgagat tgcgaccggt caccgtgaat cacagcggcg
     6481 gcgtatcctt cgcggatgag gttgcgggcc aacttgtccg ttccacgttt tgtgcgcgcg
     6541 aaaatcaggc atcggccggt ttcttttccg agcaaatgtt gaagaacttc atgcttgcgg
     6601 tcgccggaaa cttcaaaggc ttgaacacgg acattttccg ctggcttgag aatgtttccg
     6661 aatgcgagac gcaccgggct cctcatatag tccttcacga ggttcgcgac ggaagcttcg
     6721 agagtcgccg agaagcaaag cgtctggcga tctttcggca acacggctgc gatcctgcga
     6781 atggcgggaa gaaaacccat gtccagcatg cggtccgctt catccagcac aagaatgtgc
     6841 agcgtcttga agttgaacag gttgcggccc aggtaatctt ccaggcgtcc gggagttgca
     6901 acgatcaatc gtgctccagc gcgaatggct gcaagctgct tggcctcggg caatccgccc
     6961 acgaccagcg ccgctggcgg caactgcttc ccgcgcaatg cattgtattg atccacgacc
     7021 tgcatggcca gttcgcgcgt cggaacgagc acgagggctt caattccctt ggtcttctgc
     7081 gcgagcaagc gctcgaagat cgggatcagg aaggccagcg ttttgccggt gccggtctgc
     7141 gcggttgcaa gaacatcctt gccatccaat gcgactggga tcgccgcggc ttgcaccgga
     7201 gtcggcgtcg caaattttgc gaaagccaga cgctccttta tgtaattgga aatctgtaat
     7261 tctgtgaaat tctgcatgtt tctattttgt cttttctgct ggtggaagct gttgccgtac
     7321 acacgcacgc ttagtcagtg ggctctcaaa ggagcctgca cccgtgcggg cacacaactg
     7381 gcatggccag tcctagtttt aattacgctg aaaacgtacg gaggacataa gaggcggggt
     7441 tatctcgaat caatctctac cagaggaacc acaccaaaca accgatgtat taagcgcgaa
     7501 tgcactatac cacatctggc aaggaaatcg tgctaaatat ttacatggcg ggaaattttc
     7561 aatttcccgc catgcaagta ctgcaatcaa actggttagc ctagcttttc cggacttcca
     7621 gcggctccat taatttaaag cgcagcctcg tttccgggcg aatcgtaacg tcgcgatctc
     7681 cggtaagcgc cgttcccgcc gtgccggccc ctgcgcccgc tcctgcgcca attgccgctc
     7741 ccttgccgcc gcctgccagc gcaccgatca atcctccgac gattgcaccg ccacctgcgc
     7801 cttcggcagt ccgcttgccc cgacctttgc cgctttgggt gatttcagac acatttacat
     7861 cgtatttctt cccattcaga gtgactgaag tcagggtaac gctgatcgcc gcattcccct
     7921 taaaccgtcc tgcggattta gcactggtaa ccgttcctac ggcccgcgtc ccctttggca
     7981 aaatcacttt atcgcctacc accaccggct cggccagcga agcgtcaaaa tggtctccgg
     8041 aattgctatc cttggagctc acgagctgat cgaccgtaac ctcgatgcta gttcccgtgc
     8101 taatcacgat aggcttagga gcaaaaatat ggccgattga cgattctgaa ttcttttgcc
     8161 cgctggcatt acttgcggta cctgggtttg agctcgacgc tgttgcagaa ctgcccgcat
     8221 ctccgttagc aggcgaattc gtggaatccg tggatgttgt ggatttattg caacccatta
     8281 tagataaaat acttataagc acgacatagc gacctaaggc tttgtgcctc atgttgaaaa
     8341 gctccttttg ggcttgggtg attgcttcaa tactagcaag tcgcaattct ggggggtacc
     8401 tgatttttgc gtcgatgtgc ccaacttttt acgcgaaatc ggccgcttgc agatcatgaa
     8461 gagtcgtggc acgctttgct agtcgacggc atccaatgga ctgccccgaa aagagggaaa
     8521 ggccgccaga ctggggagga atgccatggc aattgaaaaa acaattactc ttatgcctgc
     8581 tgaaggccct gcgccaatga agcggcactc ccgcgcacag atggcgattg ctttcggtat
     8641 tgcggcagtc tccgatgttt tttcgatctg gcttactttt gctccaccat ttcaatggac
     8701 tctggacatc gccaccgcta tcgctctttt cttgatcctt ggcaagcgct gggcgctttt
     8761 acccggtttg atcgcagaag ccattcctgg catgggtgtg tttcctgtct gggtgctggt
     8821 tgtcctttcc gtcatcgtct acgacggcat aaaaaaacct gtgcgcgcct gattcgaaat
     8881 tttctgtcaa ttttctgcat gctccttctg gaagccgctg tttttcgtgg cataggcttc
     8941 agcctgtgga atttagccct cgcaagaacc atgcccacag gctgaagtct atgccactta
     9001 actccatata agttgattgc ccgccgggag cgaaaccagt atgctttctc aagatttttg
     9061 gagattccca tgaacaaatt ttcgaattca ttttcgcggc gaaattttct tgcgggcgcc
     9121 tcgtctcttg gcgcgttcta cgctgcctca aaatttgcgc tgccgctgcc tgcattggcc
     9181 gcgccgttga tcgacgatcc gcgcattggg ccgcagccca tcgccgacaa gggttttgcg
     9241 tccgtcagaa aaattggcaa tggcttgtac gcgacgattg ccgatcgctc aaaaggattg
     9301 caggcgcgct gcaacggcgg cttcctgatc ggccgcgacg cagccatcat gctcgaagga
     9361 tttcagacga cgctgggctc cgcatttcaa ctggagacgc tacgctcggt cacgcagatt
     9421 cccattcgcg cggcgatcaa cacgcactgg catttcgacc acacactggg caattccgtt
     9481 tacggcggca cgggaattcc catctgggcg catgccgacg tgcccgcgcg catcgctacg
     9541 tattacccca agtggcaggc gcaggattta aacacgtacc tcgtgccgtg gcaaaagcgc
     9601 gtcgccgacg ccaaatccga aatgcagcgt gagcatacca agagcgacat cgaaggcctg
     9661 actgggatgt tcgttcccgt gagccagacc gttctggcgc tgccgaacca tttgctcgat
     9721 cccgcaaaaa tgcccatgga aatcgatctc ggcggcctta cggttgtcgt cgagcactac
     9781 gtcggccaca cggataccga cctgattttc cgcgtgcccg atcaaaacat tgtttacaca
     9841 ggagatttgc tgaccggtgg ccaataccca gtgaacatca acggctatcc atccaagtgg
     9901 cgtaccacgc tctcgaagtt cgcaactttc gacaaaaata cgctcttcgt tcccggccac
     9961 ggccaaatca tgggccagga aggcgtcggc gaaatgcgct cctgcttcga cgacatcgct
    10021 gagcaagccg aaaaatcata caaggccggc gtccccgtgg aagatgccgc cgagcgctac
    10081 gtagtccccg aaaaattcaa aaactatcgc caattttcgt ggggcttctg catcacccgc
    10141 acctacgaac agttttatgc tgagtggtcc ggcaagccgg taaagatttt gaattattct
    10201 taaatcgccg cccgctgaac gaactattta tttcattcaa tgggcactcc actgagcttc
    10261 cgttgtccat cagcttctaa ttaaagtttg ggctcaaaga aactgtgctc atttctgcaa
    10321 atcaaagccc attttttacc ttgactgtaa cttcgcataa tgcgaagatt tctcatgcgc
    10381 gttgttagcc ggaaggcgat taaacaagcc tgcttcgacc acggcgaatg gagcgcatcc
    10441 ttgagcgctt ggtacaagat tgccaaaaac gccgattgga aaagtttcgc tgatgttcgt
    10501 agcagttgga aaaattcgga tgttgtcgga aattttgttg tattcgatat aagtcacaat
    10561 cgatgcaggc taatcacaac cattaagtac aggtggagaa tgatttatat ccgtcgcatt
    10621 ctaagccatg cggaatacga cgagaaaggc tggcaaaaag aatgatagct gtatccgaca
    10681 aatatgcgct cgaagtcgcc tcgccgactc caatcgcctc ggcacgtcag cacgaagaat
    10741 atctttcagt cctggataag ttgacgaaca aacagaacgc aacaagagaa gaagaaaaat
    10801 atgccgaagt attaatcacc ctgatcgaga cgtatgagga agaacaccac tctataccgg
    10861 acgcatcccc cattgaaatt cttcggtcat tgatggacgc caacgatctc cgtcaaaaag
    10921 atatgatctc catttttgga tcggaaagca tcgtatccga ggtattacac aagaagcgtg
    10981 gacttaacaa aacccacatc gagaaattga gcaaacgatt ccgcgtttca cccgcattgt
    11041 ttttttagaa tttcctttcc cacgatctcc attttcgcgg gtttccacca gctagattat
    11101 tgagcgatgg aattaatgat gaatttgtgt gcatcaaatt acacaatgct cgggcgcttc
    11161 ttgccgaagc cagtgacttg ttcaaatgcg tgcgccaatt gcaggacgct gaaatcttct
    11221 ttggcgcggc cgacgatttg gatgcctact ggcaggcctt ccgtcgtaaa accggcgggg
    11281 actgacgcag cgggattgct tgtggcggag atgtaccagc aggacttcat ccaatcgatg
    11341 tagtttgaaa atttcacgcc ggcaatttcg gtgggatagg gcgagtcaac atcgaagggc
    11401 gggagctgcg tggtgggcaa aataaaatat tcgtattttt cgaggaatgc ctgaaagccg
    11461 cgccagagtt ggccgtgagc ggtttcggct tgcgcgactt ccatgccggt cagatgcaaa
    11521 ccttcttcga tctcgccttt tagcgtgtcc ttgaatgcgt ccgggtggtc atgcaggcga
    11581 gcgccataag tgtttgcgga tgttagcgcg cgcaagatgc ggaaggcgat ttctgcggga
    11641 gcgaaatcag gctcggcctg cccaacgatg cagcccagcg actcgaatgt ttttctttgg
    11701 ccgtcaatca cggcgcgaac gcgcgggtcg aagggcaggc cgccaagatc cttgaaccag
    11761 gccacgcgca cgcctttgaa attgcgctcg agcgggcgcg cgaaaatttc tcccggctcg
    11821 ttgatcgaaa gtggcgcgcg cgaatccggc cctgcgattg tgctcagcac ataggcgaga
    11881 tcggccacgg agcggcccag gcatcccgat gtgctcagag tggaccacgc gaatgccgct
    11941 ttcggattcg gcacgcggcc caccgaaggg cggaagccga cgacgttaca gaacgccgcc
    12001 ggattgcgca gcgatcctcc tgtgtccgtg ccgctggcga caggaacgaa tccacaggcg
    12061 agcgaaacag ccgcgccgcc ggatgagcca ccgcacgttt tcgtcaagtc atacggattg
    12121 cgcgttgcgc cgaaaatttt attgaaagtc tgcgagcccg cgccgaattc cggcacattg
    12181 gtcttcccta tcgtgatcgc tcccgcgcgg ctcatgcgct cgaccacgat atcgtcttcc
    12241 ttcggaacat aatccttata aagcggagat ccaaatgtgg tacgaatacc acgcgtttcc
    12301 agcaaatctt tatgcgcgac aggaaggccg tgcagcggtc ccagtttttc gtgatgcgcc
    12361 tgcatttcgt cagctttcgc tgcggcgtca gtggccatgt ccgcaacgaa agtcacgatg
    12421 gcatttacat ttggattaac gcgctcgatt tgtttcaggt gcgcggcgag cgcttcgcgc
    12481 gcggagagtt ttttctcgcg gatgagccgc gccatttcca cgacgctcat gaaacagatg
    12541 gagtcggccg tcgcgtgcgc ttctggcgcg ttcttcaaaa gaagcgcagc agctgtgccc
    12601 aacaaaattt cacggcgagt gggttcgttt tcgctcatga gcgggagttt agcatggggt
    12661 ggatttatgt ggcataggct tcagcctgtg gaattttgcc cttgcaagga ccaaacccca
    12721 caggctgaag cgtctgcgtg agaactgtaa ctttggaact tcagcggcta aagccgaacc
    12781 gattttgcag gaagtatgtc gtggctaaag ccacgaccca caacgattca gcagctcacg
    12841 ttcagactct gaagcccgca ctactgaaac ctattccaat tttctgcgcg cgaaaataaa
    12901 tagggatgat gccagcagca cgaacatgac tacgcctacc cacggagact ggcttgcctg
    12961 cggcataaat ctttccccga aggcaaaaat tttcgccggc catggcaaga aaaacatgtg
    13021 agtcagccag attggcaggc gtttgctcaa ttcaggatcc tgaagcaaat ttagaaaaat
    13081 cgccagcagg ccaaaaacgc cgcccgcagc gatcaacccg cttgaataca gggaaccggg
    13141 gctgacttcg ctgtcagcct cgctcttttg ttcggtgccg cgctcgacga tccatcgcag
    13201 gagtcctccg gcgaacatgg ccatcgtggt ggcaatcgag atgtacgaac cgacggcaaa
    13261 cggaagagag cggacgccga gtagttccac ggcaatcacg agaaacactc caagaaaaac
    13321 aagccgccac ggcaatctct gattcaggat cccgctgatg accgtggcca tcaggcgagc
    13381 ctgcggtgca gcggcctggt cgctgccgat tccctgcacc cattgcacct cgatgcgatt
    13441 tgtgacggga tcgaaaagat attttccgtc gggaatctcc gccgatccaa ttgcgttgag
    13501 cagcacgtac gttttgcctt cgtgttggaa agcattgtcc tggcgctcga cgcctgttgg
    13561 caggctatta atgtcgaggg gaatctgcat cggcttatac tgcgcgaggc cgatgttcat
    13621 caggataagc gtgccgccaa tcgcgaacga ggaaaccagc acgccaatca caagagcgat
    13681 ttgctgacga tacggcgtcg cgccaacgag atagccagtt ttcaaatctt gagaagtcgc
    13741 gccggcgatt gccgccgcga tgcaaaccac tccgccaatc gtgagcgcga gtacggcata
    13801 cgcattcgcc gtccagtgga tcacaaggaa aacagcgcag gtggccatca atgtggcgat
    13861 ggtcatgccg ctgattggat ttgatgaatt gccgatcaac ccgctgattc gcgaggccac
    13921 agtcacgaaa agaaagccga aaacgatcac gaagattgcc gcgatcaggt tggcaaaggc
    13981 tccggtctgc gcgccgggga ttggcttgaa ccgaagcaaa gcccacatca tggccagcag
    14041 cacggtggat ccaatgaata cgagacgcat ggaaagatct cgttccgtgc gatctgattc
    14101 tgccgcgcct tcgcgggctt tccgcatgtc gttcaagccg gcagtgagcg ccgaaaaaat
    14161 tgtgggcatc gttttgatca aggtgatcaa tccggacgcg gccactgcgc ctgctcccat
    14221 gggacggata taacttgccc aaagctgatc gggcgtcatc tgcggaatag ggatcgtgct
    14281 gggataaagc ggaacggtgg gagaaagaga accaaaaaat ttaattgcgg gcatcaggac
    14341 cagccaagaa atgacgccgc cggcaaacaa aattcccgac acgcgcgggc cgataatata
    14401 acccacgccg agatattccg aggtgaccgc cgcgcgaaag gatgcgccgg gaagccaagt
    14461 cggacgatac tccggctggc cgggccacat gcccagcgtg ttcatgcaga aggtataaac
    14521 gccgccgagg ccgagtcccc agaaaacgcg cccggcaaac gatccgccgc gctcgcccgc
    14581 cacgagcacg tccgcgcacg ccgtgccttc cggaaaagta aggttgccgt gctccttgac
    14641 gatgagctgg cggcgcagcg ggatcatcag caagacgcca agccagccgc cgatcagcgc
    14701 gagaaaaaaa actcgccagt attcggtgtt caaggaaaac ccaaggaaaa taagtgcggg
    14761 cagcgtgaag atgactcccg ccgcaacgga ctcgcctgcc gagccggtgg tctgcacgat
    14821 gttgttttca agaattgtcg atttgccgaa cgcgcgcaga atggaaatcg aaagaaccga
    14881 aatggggatc gaagcggaga ccgtgaggcc ggcgcgcaaa cccacataca cggaaaccgc
    14941 gccaaacaag atgccaaaca aggagccgag aattaccgcg cgccaactga attccgtcat
    15001 ggcgcttgac gcttctacat acgggcggaa ctccgcgggc ggcgccgtgg aactccaggg
    15061 tttttctgcc aaacaacacc tccgcgatcg cgtcgctaat ttcgccggca tgcgcggcaa
    15121 agataatcgt cgtcgtgttc gtccggcatt atggcccaaa acgttttacg tacggggaaa
    15181 ttttgcagaa gcgttgcagg tttatgcggc gcgggcttca gcctgtgggg tttaggcctt
    15241 gcatggacta ccccccacag actgaagagt ctatgtgaga actgcagaat ctttgtgggt
    15301 cgtggcttta gccacgacat aaagttctgc aaaatgaaat gcggctttag ccgctgaagt
    15361 ttcaattttg cagttctcac acagactctg aagcctatgc cacataaacc tatcgcggac
    15421 aaacgaagaa gatgcaagcg gtggcattca ctacgcgcaa agctgggcgc agatttcttc
    15481 gggccagttg gtcggcagcc tccgcaattt tcagaacatt ttcggaatcg atgataaggc
    15541 ggtaaagatc cgcacggcgc gagtacgaat atttttcgta cacaagattt tcctcggagg
    15601 cgatatccat taggcgctgc caaaaacttt gccggcgaaa tattctgcgg ctggatgcag
    15661 cgtctttcat gaggtccagc aaatgccggg gatttacgcc ggggccgtcg aacaccgcat
    15721 tcgtgcaaag gttcggcgaa aatgcgcgtg aaggatcggt atccggaaat ttggcgagcg
    15781 ctgcggcaag ttcggattca tccgcgtcaa atccagtcca cgtgtagcgg taaaaatgat
    15841 aaacaaccgt tccggaatgc agaagttgct gaacgcgcgt cgtatctttt cccgcaacgc
    15901 tgagaatcat gtccagaagt tcgtccaggc taaacgtttg ccgcacgaga gggcgcacgg
    15961 ttacgtatcc ggcctcttcc tccgtgtagc gaacggaaat tgtttcgggc aatggcacgg
    16021 aaggaggata cgcgggagaa ggctaaaaag tcgatatcag aaaacaaata ttcaggaaaa
    16081 gaggtaacaa taaatcgcaa tttcggtttc gacttatttc ttgctctcac gaacagttag
    16141 gccatcttgg acaagcgatc tcttacgcga tcggacagtg ttttaccatc cactgtctta
    16201 ccttcgagac gcacctttgc agccttgatg acggcgccca tggatttcgg agaatttgcc
    16261 cccgtttcca ggattgccgc ttcgattgcc tcattcatgt ccgcatcgga agcgcctgcg
    16321 ggcaggtaat cttccagaat tgcgatttcc tttgtttcct tgtctaccaa atcctgtcgg
    16381 ccgcccttca gaaattgatc cacggactcg cgccgctgct tcatcaacgt ttgcagcacc
    16441 tgcagcgcct cggcatcttc cagaggacgc accttttcaa tttccttgta cttcagcgcg
    16501 gacttgacca tgcgcaaaac gcttagccgc aactcgtcct tggacatcat ggctgcggtc
    16561 aaatcttttt gaatgcgttc catcaatgcc atcgtgatat gcgcgaatca atattcgcga
    16621 ctctcccaat tgaaaaaatg aatcatttgc cgcggagaat ttttgctttg caaaatttac
    16681 tataacacag cgtcaccacc aaaaacgcag cggcgcatgg atactcgcag cgcgcccgct
    16741 caataaaaat ttcgtttttg tgcgaaagac taaggcaaat ccacatggtc gagcagggcg
    16801 ctaccaaagc gtctagctgc ctcgtcgtca tcctcggcaa tgtagccgat gatttcagcg
    16861 aggtcgttta gcgctctttg cgagaagaag aggcggtaat ccacgtgtgc atgagcttgc
    16921 gaacttcttc aatgggagtg gttcggccag cgtcagcgtc gcggatgcct tcctcaattg
    16981 ccacaagagt ccctttgtcc tcttcttcgc ttgcggggtc cggtttgtcg agattggatt
    17041 ccttcgccat agccatgcgc tcacaatagt acgccgcagg gtcggaggca acgcttctgt
    17101 ttccgcgctt actaggatag actaagagct cccgaacctt ccgctttggg ttccttgtgt
    17161 ctcctgtgac tggaaaataa tgactgaaaa attgccatct gtagtgattg ttggccggcc
    17221 caatgtcggc aaatccacga ttttcaaccg gttgacgggc acgcgacgct cgatcgtgac
    17281 tgacgagccg ggcattacgc gcgatcgcat ttacggcaca gcgaactggc aaggaaagtc
    17341 gttcgaagtg gtcgacacgg gcggaattgt gccggatgac aaggccgcga tcccgcgcga
    17401 gatcctgcac caggcgcaag tggcgatcga aagcgctttg cagctcttgt tggtggtgga
    17461 tgcgcgcgcc ggactgacgc cgctcgacgc tgagctggcg cgcctgctgc ggcgcacagg
    17521 gaagccgctg gcgatacttg cgaacaaagt tgacacgcag atgcaggaat cgttggccgc
    17581 gccgttctac gagctttcga aagaagtgtt tcccgtgtcg gctgagcatg gctacggttt
    17641 tgacaacctg cttgacgaaa tgacgtcacg actgggcgaa gccaagcccc aggaggcaga
    17701 gtcttccgcg tcagaaaagc aaatcaacgt ggccatcatt ggccgtccaa acgtgggcaa
    17761 atcgactttg ctcaaccggc tcgcgggcgt ggagcgggcc atcgtttcag acattgccgg
    17821 aactacgcgc gatgccgtgg atacggtggt cgagcgcgaa ggccatactt accgttttgt
    17881 ggacacggca ggaattcgcc gcaggggcaa aaccaatctg gttgccgaaa aaatgagcgt
    17941 gatgatggcg cggcggcatc tggaacaggc ggacgtggcg ctgctgattg tggatgcgag
    18001 ccttggagtt accgcgaacg atgcgaacat cgccggatac gccgaggaat ctgggcgctc
    18061 cgtcatcatc gtgatgaata agtgggacct ggcgctggaa gcggcggcgg aaaaagcgga
    18121 gcgcgaactg gaacatgagg gtaggaagcc gcatgtggcg gtcgcgcgca cgcgcaagca
    18181 cacttccgtg gtgcgcaaaa aagcggtgac gggaaaagtg gatcccaaac agcttgccga
    18241 agattacgaa aagattgtgc gcacgcgatt taaatttttg gattacgcgc cgattgtttt
    18301 tgtttccgcg aaaaccggag atcatgtcga aaaattattc gcgctgatta acaagctggc
    18361 cgatgcgcgg cgcaggcgta tcacgacggg cgagctaaac cgctgggttt cggaagtcga
    18421 tctcgatcgc ggaacttcgc cggctgcgcg cagagtgaaa atttactacg ttacgcaggc
    18481 agggacttcg ccaccgacgt ttattttatt caccaaccaa tccaagcggc tgcatttcag
    18541 ctacgagcgt ttcctcgaaa atttattgcg aaagagtttc gattttacag gtacgccgat
    18601 ccgctttttg cagcgcatga aggaaagacg cgattggaca aagcccacct ctcggcgcac
    18661 cccggaaccg aagcatcccg gcatgagcca taaccgggtg aagagggcgc cgcctgcgga
    18721 ttgaaaacgc agtttttgta gcgcccgcct tcaggcaggc accatggagt caaacaaatg
    18781 cccgcctgaa ggcgggcgct acaggaacca aatgcgaatt gtcatgcagt ttctgaagac
    18841 cgttttcact ggggcgttga cactggctgg aatcggatta ggattagggc tggagttacg
    18901 caaggacacg cggcaatgga agaaatcgaa gacaacggca acgccaacgc gcatcgtcac
    18961 gtcagcgatg gcggcgattt agaaccggga attccgggaa agaatctgtt ccggatcggg
    19021 gaagtcagtc gcctgaccgc tacaaaacct tttgtgctgc gttattggga gacggaattt
    19081 cccatgcttc agccggtaaa aagtccgaag gggcaccggc tctacaggca agaggacatc
    19141 gacacggtct tcacgatcaa acgcctgttg tacaacgaag gatttacgat tgcgggcgcg
    19201 cgacggcatt tgcgcgacca ggcattggga acaagcgggg gcgtcggcac gaatggcggc
    19261 gcggtatcag tggccgcgga gcacgaaacg cagggtaatg aatccgcatc gcaggcggcg
    19321 cctgccctat cgccgcacct cgctgtattt aaccgccaga ccctgctgga cttgcgagac
    19381 tcgctccgct cgttgttgac ggctctggaa tccgagtgat acgctgacac gttagaaatt
    19441 gttgtagccc gcctcttcag aggcgggggt ttaaatgtca cgtcatctaa accccgcctc
    19501 tgaaggggcg ggctacagta agagctttgc cttgcaatgc agacagtcgg gacgtagcgc
    19561 agcctggtag cgcgcacgct tggggtgcgt gaggtcgctg gttcaaatcc agtcgtcccg
    19621 accattttct cctcgcacga ttttgagtct gaccgccctg caacttcaca gcttacactt
    19681 taagatatga cccgctgtct tcggatgtcc gggtcttttt ctcttttggt gcctgtttac
    19741 cgctgtggta atgtgccgtg tcatggaaaa ggaatacatt ctgcaagaaa tcaagagaac
    19801 ggcagaagcc aatggtggcc tgccgctggg ctggcgcaaa ttccttaagg agacaggcat
    19861 cagggaaagc gattggctgg gaaaacattg ggcaagatgg agt
//
LOCUS       DASZMB010000126        12346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3677566, whole genome shotgun sequence.
ACCESSION   DASZMB010000126 DASZMB010000000
VERSION     DASZMB010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 12346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12346
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3677566"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /gene="gnd"
                     /locus_tag="VG272_05775"
     CDS             <1..759
                     /gene="gnd"
                     /locus_tag="VG272_05775"
                     /inference="COORDINATES: protein motif:HMM:NF007161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HWF13223.1"
                     /translation="LAGKLGKQKAIWIMVPSGAPVDQTIAELIPHLSKNDILIDGGNS
                     NYKDTQRRGVSLKEKGFQFVDVGTSGGIWGLKEGYSMMIGGDKSAVDYLRPIFETLAP
                     GKGLGWGHVGPTGAGHFVKMVHNGIEYGMMQAYAEGFAILKKKNEFALDMHQVSEIWR
                     TGSVVRSWLLDLLSIALKENAEMAGIAPYVSDSGEGRWTVAEAIDLDVPAPVITLSLL
                     QRLTSRDTDSYADKLLAAMRNQFGGHAIKAAAKK"
     gene            769..2316
                     /gene="zwf"
                     /locus_tag="VG272_05780"
     CDS             769..2316
                     /gene="zwf"
                     /locus_tag="VG272_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HWF13224.1"
                     /translation="MTADKSATIESAAEENHRSIRPADPCTIVIFGASGDLSRRKLVP
                     ALYGLAAQNCLARRFAIIGFARTAMTDEAFQNTAIESVKKFADESTLREAECKEFANA
                     LAYVSGEYDHPEAFEKLKKRLEELDSAHNLNGNRLFYLATPPEIYPTIIEQLEKAGLS
                     RNPNGKSWTRVIIEKPYGRDLASAHKLNETVRKAFDESQVYRIDHYLGKDTVQNLMVL
                     RFGNGIFEPLWNRNYVDHVQITTAETLGVEQRAAFYETAGATRDMIQSHVLQLTSLVA
                     MEPPAAFDATAVRNEKIKVLQSIRPFTPESIKSEIVRGQYGPGTSEKDGSKLAGYRDE
                     PRVAKDSGTETFVAAKLQIDNWRWAGVPFYLRTGKRLPSRVTEIAIQFRRAPHLVFRG
                     QDLSSNALVLNVQPDEGISISFHAKLPGQEMKLKTVTMDFSYQAAFGGGERSAYATLI
                     NDCMRGDATLFDRADGVEAAWALVDPILHYFESNKPKFPNYSAGTWGPHEADELLERD
                     GRHWRNS"
     gene            2291..3151
                     /gene="pgl"
                     /locus_tag="VG272_05785"
     CDS             2291..3151
                     /gene="pgl"
                     /locus_tag="VG272_05785"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HWF13225.1"
                     /translation="MADTGGIHNEGVPENPSPDVQVFPSPEEVARAAARMFVDYAWQS
                     IAKDGQFIVALSGGNTPRLMFEFLASDQFRAQVDWAKVHVFWGDERAVPPTNSESNYG
                     MARRELLIRVPIPEANVHRMEAEKASIGRAAHEYEEALRKHLPLDDRGFPQFHLIFLG
                     MGNDGHTASLFPGPRVTRQTSRWVSTPTVTKLNMRRMTLTLPVLDAAMRVVFLVVGAE
                     KAQMLHEVLEEKHDPPYPAQLVQPRHRGVKIFLVDKAAASKLTAAVTEKSVSQDRPAA
                     AGRDRSGRNK"
     gene            3148..4149
                     /gene="glk"
                     /locus_tag="VG272_05790"
     CDS             3148..4149
                     /gene="glk"
                     /locus_tag="VG272_05790"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HWF13226.1"
                     /translation="MILAGDVGGTKCNLALYDIHGVSAKKIFTRRYESRDFPTFEQVI
                     AKFLADSKAETHTSGDQTIEAAGFGVAGPVIDRRVKATNLPWIVEAAALSAQLKTRHV
                     VLLNDLEATGYSLSHLPASEISILNRGIPTPNLTQALLAAGTGLGETILFWDGSKYVV
                     SSTEGGHVDFAPRTEIEVELLRHMKKTEEFVSVELILSGRGFRTIHNFLDPSVEHPSF
                     EDPHADAAPEITRLGMAGQCPVCVKTLDLWVGMYGSEGGNLALKVLARGGVWVAGGIA
                     PKIQKKMEDGMFFKAFCAKEKFGELLAQIPIYMVLNEEAPLIGAQSQAMKAAGIQLP"
     gene            complement(4189..4776)
                     /locus_tag="VG272_05795"
     CDS             complement(4189..4776)
                     /locus_tag="VG272_05795"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWF13227.1"
                     /translation="MNAKTLVIFALLATFTSAQSARAQELLVQVDPTQSKVEYSLAST
                     MHTVHGTFAVKNGSVHYDPASGQIGGTIVVDATSGQSGNSSRDNRMHHEILETDKFPE
                     IVFSPTRLTGVVAADGISKVEVSGRFRLHGQEHDVTLPAEIKAEGKNLSITAHMDIPY
                     IQWGLKNPSNFLLHVSDTVKIEIQSTGHVVPTGNP"
     gene            complement(4815..5924)
                     /locus_tag="VG272_05800"
     CDS             complement(4815..5924)
                     /locus_tag="VG272_05800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWF13228.1"
                     /translation="MITRRELIVVGAGPAGIVAAIAAQKRGQQVIVLDARKPPIDKPC
                     GEGILPQGVAALRTLGISLPAGETFPFRGIRFADENHSARADFADETGLSVRRVKLHQ
                     LLTDYAMEAGVEFRWESRVTQIDERQVTTAKESFSYDWLIGADGHNSRIRKWAGLEPR
                     AASKRRFGFCSHYQLEPWSDVAEVHWAHRCQIFITPMRGQEVGVAVISRDPQLRFNKA
                     IQQFPFLANKLCGAAATTRELGDTTGLIVLPRVTNGRIALIGDASGTVDAVTGHGLSL
                     SFQQALSLAESMQERDLRQYEHAHKKIASLAITMTRLMMLMSESDWIRRRTLRLFQIT
                     PRLFTRMLAIHTGSVPLSTVGVAEIADFGWRFLRA"
     gene            complement(6041..6919)
                     /locus_tag="VG272_05805"
     CDS             complement(6041..6919)
                     /locus_tag="VG272_05805"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HWF13229.1"
                     /translation="MKTKHIARAFEVKDGSRFRLKDFDPAETLDLKSKEHAQDALERG
                     IARLSELQEKLYAQDRWAILMNLQAMDAAGKDSTIKHVMSGVNPQGCQVHSFKAPSAE
                     ELQHDFLWRTTRSLPERGGIGIFNRSYYEEVLAVRVHPEFLTKEKVPAELTAKNIWEK
                     RFEDINAFERYLSRNGYKVLKFFLYVSKREQKSRFLDRLDEDDKNWKFSISDVQERKH
                     WDKYMHAYEDMIRNTSTEHAPWYVVPADHKWFSRLVVAEVIVHTLQSLNLAFPKLDAA
                     KRKELKGAREALERTN"
     gene            complement(6960..7283)
                     /locus_tag="VG272_05810"
     CDS             complement(6960..7283)
                     /locus_tag="VG272_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWF13230.1"
                     /translation="MPLVKFISASGLTREVDVPVGMSVMQAALIHKIDGILGECGGNC
                     MCATCHAYVDPSFSNRIPSAKENEKFMLSIAAEGPQSNSRLTCQIKMTEELDGIAIRL
                     PKKQK"
     gene            7758..9191
                     /locus_tag="VG272_05815"
     CDS             7758..9191
                     /locus_tag="VG272_05815"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HWF13231.1"
                     /translation="MQNMNSIPNGNPKPAIRNKNANPKRQILLRGLFVLTCSLTAVYA
                     LQAQNQAHDPGPRTGTPAAGNPIAGLTSSQQSYFLDGQTRFQNVESVQNGKNNGLGPS
                     FNSNSCSSCHAQPSVGGTSPSVNPQIAVANAEGATNSVPEFITLNGPVREARFKFAVD
                     QNGRATNSPDGGVHGLFTIKGRSDAASCQMSQPNFEQMVQQNNVSFRIPTPTFGLGLI
                     ESISDDAIVANEVAQQSAGRSLGISGMANRSGNDGSITRFGWKAQNKSLVIFSGEAYN
                     VEMGITNELFPNERGYPGTPIPAGCMINPTPEDATNFDVSTTVAGPSDIVAFSNFMRF
                     LDQPQPSCTGTSCSQSIQNGRGLFIAAKCSVCHTPSMTTGPAYVAALSSVPVNLFSDL
                     LVHHMGNGLADGVSQGAAGPDQFRTAPLWGLGQRLFFLHDGRTPDLIQAIEDHASPGS
                     EANQVIRVFNQMSQSQKQDLLNFLRSL"
     gene            complement(9484..9807)
                     /locus_tag="VG272_05820"
     CDS             complement(9484..9807)
                     /locus_tag="VG272_05820"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWF13232.1"
                     /translation="MSETPYAVARTSPRFTFIADAEVIGCTGSARVSELSARGCYIDS
                     INPLPKDSQVRIRIRYGCSTCVFSGTVIYTHAGFGMGVSFAELTGEQRSTLDVWLDEL
                     ARKLV"
     gene            10079..10828
                     /locus_tag="VG272_05825"
     CDS             10079..10828
                     /locus_tag="VG272_05825"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWF13233.1"
                     /translation="MNSIASAFACAALFSFAAGAGAQSARPVQTFQTSKGPVKITPVY
                     HASLEIEAGGKVIIIDPAKPANFSGLPQADLVLITDIHGDHMDADLLKAESKAGTEVI
                     APAAVAKTVTTAKVIGNGENTTWGDWKIEAVPMYNTTEGRGPGPGKFFHDKGRGNGYV
                     LTYGGKRFYFSGDTEGIPEMRALKNIDVAFICMNLPYTMTPDEAADAVKAFHPKVAIP
                     YHYKGQDTAVFKKDLEGTGIDVRLLEWYPAA"
     gene            complement(10937..12271)
                     /locus_tag="VG272_05830"
     CDS             complement(10937..12271)
                     /locus_tag="VG272_05830"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HWF13234.1"
                     /translation="MMKMRQLIVATVVLAALAAGLYWSNHRKPAEDSAATTPSVSNAK
                     VISLNQDDISKLEVKKKDGDVVLDRVSAANWKITSPKPLVADQSSVSTILYNLAPMDG
                     ATLIDDHVTDLKQFGLTDPDAKVTATLKDGKSQTVLIGDETPTGDAAYVMVSGESKVY
                     SIPKNTKTNLDKSLSDLRDKRLLPVDFDKLRSVDISGGKLHLAFASQDGQWIVRSPAN
                     LRGDTSMMETIIEKLRTATMDPSAAEAETKKAASSFGSGTPVANIKATDASGTQELQV
                     RKAGDAYYAKTTAMDGAFKVPNELGEAVNKDAEDFREKRVFDFGADDPDKVEYHDGPK
                     AYSLTRSGEDWSSDGKKMDAVAIQEFLRTIRGLTATKFATSGFASPTISIVATSKDGK
                     HVEKVDIAKGGSGYLAKRADGPSLFVLDEKAVEDMQKAAGDLKPAEAPPVKK"
BASE COUNT         2819 a         3283 c         3424 g         2820 t
ORIGIN      
        1 ctcgcgggga agcttgggaa gcagaaagca atctggatca tggttccgtc gggcgcgcca
       61 gtggaccaga cgatcgccga gctgattccg cacctttcaa aaaatgacat tttgatcgac
      121 ggcggcaata gtaattacaa agatacgcag cgccgcggag tgagcctcaa agagaaggga
      181 tttcagtttg tggatgtcgg cacgagcggc gggatctggg gactgaaaga aggctactcg
      241 atgatgatcg gcggcgacaa atccgccgta gattatttgc gtccgatatt tgaaacgctc
      301 gcgcctggaa aaggtttggg ctggggacat gtgggtccta ccggtgcggg ccattttgta
      361 aaaatggttc acaacggaat tgaatatgga atgatgcagg cctacgccga aggtttcgca
      421 attctcaaaa agaaaaatga attcgcgctg gatatgcatc aggtgtctga gatttggcgc
      481 acgggcagcg tcgtgcgttc gtggctgctg gatttgctga gcatcgcgct gaaggaaaac
      541 gcggaaatgg cgggaatcgc tccatacgtg tcggactcgg gagaaggccg ctggactgtg
      601 gccgaggcga ttgatctgga tgttcccgcg cctgtgatta cgctatcttt gctgcagcga
      661 ctgacgtcac gcgatacgga ttcgtatgcc gacaagctgc tggctgcgat gcgcaaccag
      721 tttggcgggc atgcaattaa agccgccgca aaaaagtgag ggcccgacat gacagcggat
      781 aaaagcgcaa cgatcgagag tgccgcggag gaaaatcaca ggtccatcag gccggcggat
      841 ccgtgcacga ttgtgatttt cggcgcgtcg ggcgaccttt cgcgccggaa actcgtgccc
      901 gcgttgtacg gtcttgcggc gcagaattgc ctggcgcgaa gatttgcaat tatcgggttc
      961 gcacgaactg cgatgacgga tgaagctttt cagaatacag ccatcgaatc cgtaaaaaag
     1021 tttgcagacg aaagtacgct gcgagaagcg gagtgcaagg agtttgcgaa tgccctcgca
     1081 tatgtttccg gggaatacga tcatcccgaa gcgtttgaga aactaaagaa gcgtctcgag
     1141 gaattggaca gcgcccataa cctgaatgga aaccgcctgt tttatttagc gaccccgccg
     1201 gaaatttatc ccaccatcat tgaacaattg gaaaaagcag ggctctccag gaatccgaac
     1261 ggcaaatcgt ggacgcgggt cattatcgaa aagccatacg ggcgcgatct cgcatccgcg
     1321 cacaagctga atgaaaccgt tcgcaaggcg tttgacgagt cgcaggtcta ccggatcgat
     1381 cactatttag gaaaagatac ggtgcagaat ctgatggtgt tgcgcttcgg caacggaata
     1441 tttgagccgc tgtggaatcg caattatgtg gatcacgtgc aaatcacgac tgccgaaacg
     1501 cttggcgtgg aacagcgcgc ggcgttttat gaaaccgcgg gcgctacacg cgacatgatc
     1561 cagagccacg tgctgcaatt gacttcgctc gtggccatgg agccgccggc ggcgttcgat
     1621 gcgactgctg tgcggaatga aaagataaag gtcttgcaat ccatccggcc cttcaccccg
     1681 gagagcataa aaagcgagat tgtgcgcggg cagtacggtc cgggaacttc cgagaaagat
     1741 ggttccaagc tcgcgggtta ccgggacgag cctcgtgtgg caaaagattc gggaacggag
     1801 acattcgtcg cggcaaaatt gcagatcgac aactggcgct gggccggcgt gccattttat
     1861 ctgcgaacgg ggaaacgtct gccttcccgg gtcacggaaa ttgccataca attccggagg
     1921 gcgccgcatc tcgtgttccg gggccaggat ctgtcgtcca acgccctggt gctgaatgtt
     1981 cagcccgacg aaggaatttc gatttcattt cacgccaagt tgccggggca ggaaatgaaa
     2041 ctaaaaacgg tgactatgga ttttagttac caggcggcat tcggcggcgg cgagcgttcg
     2101 gcatacgcga cgctcatcaa cgattgcatg cgcggcgacg cgactttgtt cgaccgcgcc
     2161 gacggcgtgg aagcagcgtg ggcgctggtg gatccgatcc tgcattattt tgaatcgaac
     2221 aaacccaaat ttccaaacta ctcggcgggc acatggggcc ctcacgaagc cgatgaattg
     2281 ctggagcgag atggccgaca ctggcggaat tcataacgaa ggcgtgccgg agaatccgtc
     2341 tccggatgtg caggtgtttc caagtcctga ggaagttgca cgagccgcgg cgcgaatgtt
     2401 cgtcgactat gcctggcaat cgattgccaa ggatggtcaa tttatagtgg cgctgtccgg
     2461 cggaaatacg ccgcgcctga tgtttgaatt tctggcgtcg gatcaatttc gcgcgcaggt
     2521 ggactgggcc aaggtccacg tgttttgggg agatgagcgg gctgtgccgc ccacaaactc
     2581 ggaaagcaat tacggaatgg cgcgccgcga attgctgatc cgggtgccga tccctgaagc
     2641 caatgtgcac cgcatggaag cggaaaaagc cagcatcggc cgcgccgccc acgaatacga
     2701 agaagcgctc aggaagcatc ttccactgga cgaccgcggc tttccgcaat ttcacttaat
     2761 tttcctgggc atgggaaatg atgggcacac ggcctcgttg tttccggggc cgcgcgtcac
     2821 gcgccaaact tcacgctggg tcagcactcc cacggtaacc aaattaaaca tgcggcgcat
     2881 gacgttgact ctgcccgtgc tggatgcggc gatgcgcgtt gtctttttag tcgtgggcgc
     2941 ggaaaaggcg caaatgttac acgaagttct ggaggaaaaa cacgatccgc catacccggc
     3001 tcaacttgtc caaccgcgcc atcgcggcgt aaaaatattt ctggtagata aggcagcggc
     3061 gtcgaagctg accgccgccg tcaccgaaaa atcggtttcg caggacagac cggcagctgc
     3121 aggccgcgac aggtctggga gaaacaaatg attctggccg gggacgtcgg gggcaccaag
     3181 tgcaatctgg cgctttacga cattcatggt gtgtctgcta aaaaaatctt cacgcgtcga
     3241 tatgaaagcc gagatttccc cacctttgag caagtgattg cgaaattcct ggcggattca
     3301 aaagccgaaa ctcacacatc cggagaccag acaatagaag cggcgggttt cggcgtagcc
     3361 gggcccgtga ttgatcgcag agtaaaagcc accaacctgc cgtggattgt ggaagccgcc
     3421 gcgctttctg ctcagttaaa gacccggcat gtcgttttat tgaatgatct ggaagcgacg
     3481 ggatatagtt tgtcgcacct gccagcgtct gagatttcca ttctgaatcg agggattccc
     3541 actccgaatc tgacgcaagc tttgctcgcc gcgggcacgg gactcggcga gacaattctg
     3601 ttttgggatg gcagcaaata cgtcgtttcg agtaccgagg gcggccatgt ggattttgcg
     3661 ccgcgaacgg aaattgaagt cgaacttctg cgccacatga aaaagactga ggaatttgtg
     3721 agtgttgaat taattctgtc gggccgcggt tttcgcacga ttcacaattt tctggatccc
     3781 tctgtcgagc acccatcctt cgaagatcca cacgcggatg cggctccaga aatcacgcgt
     3841 cttggaatgg ctggacagtg cccggtgtgc gtaaaaactt tggatctgtg ggtgggcatg
     3901 tacgggtcag aggggggcaa cctcgcgctg aaggttctgg cgcgtggagg cgtttgggtt
     3961 gccggaggta ttgctccgaa aattcaaaaa aagatggaag acggaatgtt tttcaaagct
     4021 ttttgcgcca aggaaaaatt cggcgagctg ctggcgcaga ttccgattta tatggtgttg
     4081 aatgaagaag cgccgctgat cggggcgcag tcccaggcta tgaaagccgc gggaattcag
     4141 ctgccctgac gattattgct tgtcattgct tatcgctgct tcagtaaatc aaggatttcc
     4201 ggtcggtact acgtggccag tcgactgaat ttcgattttc actgtatcgc taacgtgtaa
     4261 aagaaagttg ctgggatttt tcaggcccca ctgaatatag ggaatatcca tgtgcgcggt
     4321 gatggaaaga ttctttccct ctgcttttat ttccgcggga agcgttacat cgtgctcctg
     4381 cccatgtaga cgaaagcggc ccgaaacttc cactttggaa atgccatccg cggccaccac
     4441 gcccgtaagc cttgtgggag agaaaacaat ttcaggaaat ttatcggttt ccagaatttc
     4501 atggtgcata cgattatcgc gcgagctgtt gccactctgc ccgctcgttg catccaccac
     4561 gatcgtgccg ccgatttgtc cgctcgccgg gtcataatga acggagccgt tcttaacggc
     4621 gaatgttccg tgcaccgtgt gcattgtgct ggcaagggaa tattcgacct tggattgagt
     4681 cggatcgact tgcaccaaca gttcctgcgc acgcgccgat tgcgctgacg tgaaagtcgc
     4741 cagcagtgcg aagatcacca gggtcttagc gttcataatc tctgtgattg ttcgagagga
     4801 tcgattcatg taggtcaggc ccgcaaaaat ctccagccga aatcggcgat ttcagctacg
     4861 cccacggtag aaagcggcac ggagcccgta tgaatcgcca gcattctggt gaagagcctt
     4921 ggggtgattt gaaacagtcg aagagttctg cgacgaatcc aatcgctctc gctcatcagc
     4981 atcattagcc gcgtcatggt gatggcaagc gaagcgattt ttttgtgggc gtgttcgtac
     5041 tgcctcaagt cgcgctcttg catgctttcc gccagcgaaa gagcctgctg aaaggaaagg
     5101 ctcagtccgt gcccggttac cgcgtcgacc gtgccggatg cgtctccgat gagggcaatg
     5161 cggccattcg tgactcgcgg caagactatc aagccggtcg tgtcgcccag ttcgcgcgtg
     5221 gtcgccgcgg ccccgcataa tttattcgca aggaaaggga attgttgaat ggctttattg
     5281 aagcgcagtt ggggatcgcg agagatcacc gcgacaccca cttcttgccc ccgcatgggc
     5341 gtgatgaata tctggcagcg atgtgcccaa tgaacttctg ctacgtcgga ccacggttca
     5401 agctgatagt gactgcaaaa tccgaatcgt cgcttgctcg cagcgcgagg ttccaagcca
     5461 gcccacttgc gaatgcgcga attgtgacca tccgcgccga tcaaccaatc gtacgaaaag
     5521 ctttcctttg ctgttgttac ttggcgctcg tcgatttgcg tgactcgcga ttcccagcga
     5581 aattcgactc cagcctccat ggcgtagtcg gtaagcaact ggtgcagctt cacgcgacgc
     5641 acagacaaac cggtttcatc cgcaaaatca gcgcgcgccg aatggttctc atctgcgaag
     5701 cgaattcctc gaaatggaaa ggtctctcca gccggaaggg aaatgcccag cgtccttaac
     5761 gcagctacgc cctgcggcaa aatgccctca ccgcatggct tgtcgatggg aggtttacgg
     5821 gcatccagca cgatcacttg ctgcccgcgc ttttgcgcag caatcgctgc aacgattcct
     5881 gcgggtccgg ctcctacaac gatgagttcg cgacgcgtaa tcacaattac gagtctgatg
     5941 ccgtccgttg gcgcggagtt gttctgaaag caaaaaaccg cgaaaaaatt ctgcaagcct
     6001 cgtggatgta acgagtgagc ggtgtatcac acgtgctgtg ttaatttgtg cgttcgagtg
     6061 cttcgcgagc ccccttcaat tctttgcgtt tcgctgcatc gagctttgga aatgcgaggt
     6121 tcaacgattg cagcgtatgg acgatcactt ccgcgacaac gaggcgcgaa aaccatttgt
     6181 ggtctgcggg cacaacgtac cagggcgcat gctcggtgct cgtgttacga atcatgtcct
     6241 cgtacgcgtg catatacttg tcccaatgtt ttcgctcttg aacatcactg atcgagaact
     6301 tccagttttt atcatcctca tccagtcgat cgaggaagcg cgacttctgc tcgcgcttcg
     6361 atacatagag aaaaaatttc agcaccttgt agccgttcct ggaaaggtat cgctcaaacg
     6421 cattgatgtc ttcgaatcgt ttctcccaga tgtttttggc cgtcagctcc gcgggaactt
     6481 tttctttggt taagaattcc ggatgcacgc gcactgccag aacttcctcg taatacgacc
     6541 tattaaagat tccgattccg ccccgttcgg gaagggatcg cgtggtccgc cacaaaaaat
     6601 cgtgctgcaa ttcttccgcg gatggcgctt taaatgaatg cacctggcag ccctgcggat
     6661 ttactcctga catcacgtgc ttgatcgtac tgtcctttcc agccgcgtcc atggcctgaa
     6721 gattcatcag aatcgcccaa cgatcctgtg catacagttt ttcctgaagt tccgacaggc
     6781 gggcgattcc gcgttccaaa gcgtcctgcg cgtgctcttt tgatttcagg tcaagcgttt
     6841 cagccggatc gaaatctttt aaacgaaaac gcgacccgtc ttttacctca aatgcgcgag
     6901 caatatgttt tgttttcatg attacgccgc acctttaccg tcaaagcata tttatagagt
     6961 tatttctgct tcttaggaag gcggatcgca attccatcca actcttccgt cattttaatc
     7021 tggcaggtca gcctgctgtt cgattgggga ccctccgccg cgatgcttag catgaatttt
     7081 tcattttctt tcgcggacgg aattcgattt gagaacgacg gatcaacata cgcgtggcag
     7141 gtggcgcaca tgcaattgcc gccgcactcg ccgagaatac cgtcgatctt gtgaatcaac
     7201 gctgcctgca ttacggacat gccaaccggc acatcaactt ctcgcgtaag cccacttgct
     7261 gaaataaatt tcactaaggg catcccgtgt cctctggtct gatctggatg gtaacgagca
     7321 tttgttagtc taattgggac aagcaaaccc tgtcgagcga tctcacccta acgcggattg
     7381 ctaattagag acgtcgcccg aaacacggtc cagttcgaag ggcaggcaat ttccagtcct
     7441 cactacctgt ccttacccat cagaccgatt ctgtacttcg gaacaggcgt atctcaatgt
     7501 gaattcaact accctttcac tccgttaccg gggcggggga aagctaaagg tttttcttcg
     7561 aacaaatcct ttcacacgaa tctctgtatt cgctcgtcgt cgacttccgt atttcggagc
     7621 gtattccggc gcagaaatat atttggcgtc agatgaattt gtgcggcgcg gaacgatgga
     7681 ggtgcaaaca gggctcttca gatcagcaac acaatttcgg ggttcgcccc agtccaaaaa
     7741 cctaaggggt aaatgaaatg caaaatatga acagtattcc gaatggcaac cccaagccgg
     7801 ccatcaggaa caagaatgca aatccaaaac gtcagattct tcttcgcggt cttttcgtcc
     7861 tgacctgttc ccttacagcg gtttatgcct tgcaggcgca gaaccaggct cacgacccag
     7921 gaccgcgcac cgggacgcct gcggcgggca atcctatcgc cgggctcact tcgtcgcagc
     7981 agtcgtattt tttagatgga caaactcggt ttcagaatgt ggaaagcgtt caaaacggaa
     8041 aaaataacgg cctgggccct tcgttcaact ccaactcctg ttcgagttgc cacgcccaac
     8101 cctcggtagg aggaacgagt cccagtgtga atccgcagat cgcagtggct aatgcggaag
     8161 gggcgacgaa ttcagttcca gagtttataa cgctgaacgg tcctgttcgg gaggcccgct
     8221 ttaagtttgc cgtagatcaa aatggacggg cgacaaacag cccggatggc ggcgtgcacg
     8281 gccttttcac gattaagggc cgctccgatg cggcaagttg tcagatgagc cagccgaatt
     8341 ttgaacagat ggtgcaacaa aataacgtct ccttccgcat tccgactccc acattcggat
     8401 tgggcctcat cgaaagtatc agcgacgatg cgattgttgc caacgaagtc gcgcagcaat
     8461 ctgctggaag atcgttggga atctccggaa tggctaaccg cagcggaaac gacggttcga
     8521 tcacccgctt cggatggaag gcacaaaaca aatcgctcgt gatcttctcg ggcgaggctt
     8581 acaacgtgga aatgggaatc accaatgagc ttttcccgaa cgaacgcggc tatcccggca
     8641 ccccgattcc cgcggggtgc atgatcaatc ccactccgga agacgcgacg aactttgacg
     8701 tgagcacaac ggttgcgggc ccgagcgata tcgtcgcgtt ctccaacttc atgcggtttc
     8761 tggatcagcc gcagccctcc tgcacgggca cgagctgttc acagtcgatc cagaatggcc
     8821 gcggcctgtt tatcgccgcg aaatgctctg tgtgccacac accaagcatg acgactggcc
     8881 ccgcctacgt tgccgcactc agtagcgtgc cggtaaacct gttctctgac ttgttggttc
     8941 atcatatggg caacggatta gcggacgggg tttcgcaagg agctgcaggc cccgatcagt
     9001 tccgcaccgc gccgctctgg ggactcggcc agcgcctgtt tttcctgcat gacggaagaa
     9061 cacccgatct gattcaggcg attgaggacc atgcaagccc cggctccgag gccaatcagg
     9121 tcatccgtgt gttcaaccag atgtcgcaga gccagaagca ggatttgctc aatttcctgc
     9181 gttcgctgta agcgcgggat gaatggcggc gtgcagggaa tcgcagaccc tgcatgccgc
     9241 cagaaatcta tgccttgtat caatgctccc gcctgaaatc atgaatccaa aataatttca
     9301 atcagaaaac ctttggcagg ccgcggttgc aaatggtagc gggcttcgta gaggccattc
     9361 cattccgtgt atttcggaac ggaacgggca tggcgctgcg agcgtggtcc aacgaatgtg
     9421 gatgctaaaa aaagttgttt cgaacaaggt atttgcccca gcggtagctg accccgagtc
     9481 tgcctagacg agcttgcgcg ccagttcatc cagccagaca tccagcgtag agcgttgctc
     9541 accagtcagt tcggcaaacg agacgcccat cccgaagcct gcgtgcgtgt agatcaccgt
     9601 tccagagaat acgcatgtgc tgcagccgta ccggattcgt atacgcactt gcgagtcctt
     9661 gggcagtggg ttaatcgaat cgatgtagca gccgcgcgcg ctcaactcag aaacacgcgc
     9721 gctcccggtg catccaatca cttccgcgtc ggcaataaac gtgaaacgtg gactcgttcg
     9781 agctacagcg tacggggttt cactcatggt gtcgtctggc tccgtgctgc gtgtgcagag
     9841 atactctgct tctattgtac gcagctgggg aggcttgggt gaaaacgcgc aaacgttatt
     9901 gctaaaaagt catctgtccc ggagaacagg gcggtacagg taagttaaca gcggagccgc
     9961 gcttataagc ccgaccggaa ggcgcgaaat acagcgtagg cgttgctgat tgaatcggtc
    10021 gcagcgcgcg tgctaagatt tcgccagttt agggtggaaa tccccacagg agtgacaaat
    10081 gaattcgatt gcttcagctt ttgcgtgtgc tgcgctgttt tctttcgcgg cgggtgccgg
    10141 tgcgcagtcg gcacggccgg tgcagacatt tcaaacatcg aagggaccgg tgaagattac
    10201 gccggtgtat cacgcgtcat tggaaattga agcgggcggt aaagtaatca tcatcgatcc
    10261 cgccaaaccg gcgaattttt ccggcttgcc gcaagcggac ttggttctca tcacggatat
    10321 tcatggcgat cacatggatg cggatttgct gaaggcggag agcaaggcgg gcacggaagt
    10381 tattgctccg gccgcggttg caaaaaccgt caccacagca aaagttattg gcaatggcga
    10441 gaatacaacc tggggcgact ggaagatcga agcggtcccc atgtacaaca ccaccgaggg
    10501 acgtggcccc ggacccggga agttctttca cgataagggc cgcggcaatg gttacgtgct
    10561 gacttatggc ggcaagcgct tctacttctc gggcgacacg gaaggcattc cggagatgcg
    10621 cgcgctcaag aacatcgacg tcgcgttcat ctgcatgaat ctgccctaca ccatgacacc
    10681 agacgaagcg gccgacgcgg taaaggcgtt tcatcccaaa gtcgccatcc cctatcacta
    10741 taagggacag gacacggccg tattcaagaa ggacctcgag ggcacaggta tcgatgttcg
    10801 cctgctcgaa tggtatccgg cagcctaaaa tctgtttgga attaagtaca caggcttcag
    10861 cctatggagt ttgaatctgc tcgaaacaaa ccccgcaggc tgaagccggt gctacgtgag
    10921 gcatctattt ttctggttat tttttcacag gaggtgcttc cgccggtttc aaatcgccgg
    10981 cagctttctg catgtcttcc acggctttct catccagcac aaaaagcgaa ggtccgtccg
    11041 cacgtttcgc cagatagccg cttccacctt tcgcaatgtc cactttttcc acatgcttgc
    11101 cgtctttgga cgttgcgaca attgaaatag ttggagaagc aaatccgctg gtggcaaatt
    11161 tggtagctgt caacccacga atggtgcgca gaaactcctg gattgccacg gcgtccatct
    11221 tctttccgtc cgacgaccaa tcttcgccgc tgcgcgtcag ggaataagct tttggtccgt
    11281 cgtggtactc cactttgtcc ggatcgtccg cgccgaaatc gaagacccgc ttctcacgga
    11341 agtcttcggc gtccttgttg acggcttcgc ccagctcgtt cggcacctta aaagcgccgt
    11401 ccatcgcggt cgtttttgcg tagtaggcgt cgcctgcttt gcgcacttgc aattcctgcg
    11461 tgcccgaagc atccgtggct ttgatgttcg cgaccggcgt acccgagcca aatgaggagg
    11521 cagctttctt cgtttccgct tccgcagcgc tcggatccat cgttgcggtg cgtaatttct
    11581 cgatgatcgt ctccatcatg gaggtgtcac cgcgcaaatt cgcagggctg cgcacgatcc
    11641 actggccgtc ctgcgaagca aaagccaaat gcaacttccc gccgctgatg tcgacgctcc
    11701 tcaatttatc gaagtcgaca ggcaagagtc gcttatcgcg cagatcactc agactcttgt
    11761 ccaaattcgt tttcgtgttc ttgggaatgg aataaacttt agactcgccc gaaaccatga
    11821 cataggctgc gtcgcccgtt ggagtttcat ctccgatcag gacggtttgc gattttccat
    11881 ccttcagtgt ggcggtaact tttgcgtccg ggtcggtcag gccaaactgt ttcaggtccg
    11941 ttacatgatc atcgatcagc gtcgcgccgt ccataggagc caagttgtac agaatcgttg
    12001 aaacggaact ctgatcggcg accagcggct tgggggaagt gattttccaa tttgcggcgg
    12061 aaacgcggtc gaggacgaca tcgccgtcct ttttcttcac ttccagtttg gaaatgtcgt
    12121 cctgattgag cgaaattacc tttgcgttgg aaacgctggg agtcgtggca gcggaatcct
    12181 cggcgggctt gcggtgattc gaccaataca gccccgcggc gagtgctgcc aacactacgg
    12241 tcgcaacgat gagttgtcgc atcttcatca taatttttat ctcacagtga atgggatgag
    12301 atcgaagctg ccgctatctg cgccgccacc atacggagag acctgc
//
LOCUS       DASZMB010000127        18593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3143357, whole genome shotgun sequence.
ACCESSION   DASZMB010000127 DASZMB010000000
VERSION     DASZMB010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 18593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18593
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3143357"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1560
                     /gene="glgB"
                     /locus_tag="VG272_05835"
     CDS             <1..1560
                     /gene="glgB"
                     /locus_tag="VG272_05835"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HWF13235.1"
                     /translation="PNTGSIVTRLDHHKWSDAGWMDWRSRNDWLSSPISVYEIHLGSW
                     RRNTQDTWRWLSYGELADQLIPYLKQLNYTHVELMPVMEHPFDGSWGYQTLGYFAATS
                     RYGTPADFMAFVDRFHQAGIGVLLDWTPAHFPSDGHGLGEFDGTHLYEHADPRQGRHP
                     DWGTLVFNYGRNEVQNYLISNALFWIDKYHIDGLRVDAVASMLYLDYSRKEGEWVPNE
                     FGGRENLAAIAFIKRLNEVIYARHPGVLTIAEESTSWPMVSRPTYLGGLGFSLKWNMG
                     WMNDTLSYFAVDPIYRKYHHSRMTFSMLYAFTENFALPISHDEVVHGKGSLINKMPGD
                     LWQQVANMRLFFAYMIAHPGKKLMFMGSEFGQRSEWNPDASLEWFLLEYDTHRGLQTL
                     TADLNSIYRNERSLHEVDFDWHGFEWIDCNDADSSVLSFIRRAKDPADFMIVVANFTP
                     VARETYRVGVPEPGFYREIMNTDAEKYAGTNMGNLGGVHAEAIPWNNHPYSIHMRMPA
                     LAVIYFKIQRW"
     gene            complement(1613..2404)
                     /locus_tag="VG272_05840"
     CDS             complement(1613..2404)
                     /locus_tag="VG272_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWF13236.1"
                     /translation="MPPAGSKFFAARILERREISPDLWAIRVDAGGEFPYRSGQYATL
                     GVVTPEKHHERPYSIVSAPHEKTLEFFFELVPHGQVTPRFHALRAGDEISLRKSAKGN
                     FTLDVSGQCTNHFLVSTVTGVAPYVSYVRSLYCDWKSGKFAGHHKLFILDGASRSWEL
                     GYSKEIENVAAEAPWLTYVSTVSRPWEDTNWHGETGRVDDLIRKYSDMWNLSPDDTKI
                     YLCGHPEMVENVRGIVRRRGWLETSIQAEAYFVPPRRDYSAEADE"
     gene            complement(2555..3253)
                     /locus_tag="VG272_05845"
     CDS             complement(2555..3253)
                     /locus_tag="VG272_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13237.1"
                     /translation="MKLKRLAVILFATLAIAIPAAAQMGMGMGGRIPTLSGVWRPVVG
                     TGASYEIAKPDGTKMPMEITIVGKEDVDGKPAYWMEMSMVDPRTSTPVYVKSLTTVNE
                     NNVVSSRMIMQMPGQDPMEMDGNMAAQARRMQQSTPSDINDKAEVVGTESVTVPAGTF
                     TCQHYRMKDGSGEAWVSDKVGPWGLVKGVNNGANKGETMVLTKVITDAKDHITGTPKK
                     FDPMQMMRNRQQGQ"
     gene            complement(3467..4585)
                     /locus_tag="VG272_05850"
     CDS             complement(3467..4585)
                     /locus_tag="VG272_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HWF13238.1"
                     /translation="MPEKQYKGFVIWDLLWSNLGVHPLRSALSVFALALQVFLVLLIV
                     GLTSGALSDWRTRAEGVGADIIVQPPNSSIFFAFSSAVMPETLGAQMSALPGVRVVAP
                     VLIAVDQKNLGVLYGIDYQQFAGLSDGFTFLSGGPFQAPDQTIVDDLAAGSRKLRVGQ
                     KVTLLGHEFTISGIVVHGKGARYFIPIKTAQDIVGAEGRVSMFFVRSTGNTDGTRSEI
                     VKLLPTFKIRSMAEFSTLMTSSNLPELKPFIRAFVILGVVISFLVVLLTMHTLVFERT
                     RDIGILRALGSSRMEVCGLILGETLVMVALGSIFGLICTYSIVAILHKTSPTLQIAIE
                     GGWILRAILLTIGGAIAGAAYPALRAAQSDPVDALAYE"
     gene            complement(4818..5240)
                     /locus_tag="VG272_05855"
     CDS             complement(4818..5240)
                     /locus_tag="VG272_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13239.1"
                     /translation="MNLGKNCVKASLRVLPFLFLILMVAANAQDKSGDTNGKADSGSK
                     DSRLTIVVTGGEDKKPIESASVYVRYVEGRKLGKDKKIEMNLKTNQSGICHVPVIPPG
                     KFLVQVIAEGWKTYGEYYDINQTEQTINVALVRPPKWY"
     gene            5575..6714
                     /locus_tag="VG272_05860"
     CDS             5575..6714
                     /locus_tag="VG272_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HWF13240.1"
                     /translation="MGFATDAIHVGQEPDPTTGAIVAPIYQTSTYIQEELGKTKGYDY
                     ARTNHPNRKALERTVAKLEGGSAAYVFTSGMAAIDSVFRLMRPGDHAIISEAVYGGVI
                     RLTTQFLIQWGLEFSFVDTSSPELVERAIKPNTKMIYVETPTNPTMCVTDIAAIAKIA
                     TDHSATMVVDNTFLSPYLQSPLALGAHIVVHSMTKYLNGHSDAVGGSVILTRAEDAEK
                     IYFLQRSTGAGLAPMDCFLVSRGIKTLAVRMKQHDANGRTVARFLEDHPRVKCVLYPG
                     LPSHPQHDVAVRQQRGFGAMISFDVVTMENARTVLNSLKVCSLAESLGGVETLISHPA
                     TMTHSSVPAEVRQRIGITDGLIRISVGIEDVEDLINDLDQALRQI"
     gene            complement(6940..7254)
                     /gene="cutA"
                     /locus_tag="VG272_05865"
     CDS             complement(6940..7254)
                     /gene="cutA"
                     /locus_tag="VG272_05865"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HWF13241.1"
                     /translation="MTDKLVVLVTCGSTAEAKRIACAVVEARLAACVNILPGTVSSIY
                     RWKGKVETARERLLLIKTSRKHLTKLQAAVERLHSYDVPEFLALPIATGSRKYLRWMD
                     EV"
     gene            7364..8785
                     /locus_tag="VG272_05870"
     CDS             7364..8785
                     /locus_tag="VG272_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13242.1"
                     /translation="MTATIKFGTSGWRAIIADEFTFANVRLATAAIAEHVRSRNPKAT
                     LIVGHDTRFFAEEFTHTAAEILREYGIRVLLCDDPTPTPVIAHEIIHRKVDGAINFTA
                     SHNPAEYQGLKFSGPDGGPALPEVTHDIEARAAKIGDRAPAKPNDDPFEKINPRSAYL
                     ERLTQLVNFKALGDAKLNMVTDALHGCGAGYLDEALSSHGVKVTALRTHRDVLFDGSG
                     PDVSEDNLQKTRAAVLANKAAAGLAADGDADRFGILDSDGTWMQPNHILGLLYDYIVE
                     TRGWKMPAARSVATSHLMDAVARLHGTTVIQTPVGFKYIGELIQEDKIAMGGEESAGM
                     TIRGHLPEKDGILACLLVAEMIAVRRTSLSEQLKSLFKRVGSEFWPQRTNLHLPDDVK
                     IRTVNRLKTDFKTFLGRPVKSLDRTDGLKMEFEDGSWVLLRLSGTEPLMRVYTEAATL
                     KESLQIASETEAWVNSSSQEAKA"
     gene            8782..9129
                     /locus_tag="VG272_05875"
     CDS             8782..9129
                     /locus_tag="VG272_05875"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HWF13243.1"
                     /translation="MMTLKEIREISPSEVIAYARKNTRQILALALFALFVHDIFGPHG
                     FIAMRRTQKEIDQIHEQIVKMNNENKALTDQVSSLKSDPKSIERIAREEMGLARPGEM
                     IFKIPDFAKPPAK"
     gene            complement(9303..10436)
                     /locus_tag="VG272_05880"
     CDS             complement(9303..10436)
                     /locus_tag="VG272_05880"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HWF13244.1"
                     /translation="MADTITKRIAYIDWLRGFACLVMFQTHCYDSWLGGAARDSGFFK
                     MSQLGGTLPAPMFLFLAGTSFALVTEKLRQKGVSADDVARTTILRGGEIFLLGLLFRL
                     QEFLLGQPWASWTDLLRVDILNVIGISLMMLGVACRIAAAGGATDASQLRRKTILTAI
                     VCAAAIAMLTPPLWTSWRPHSLAWWLESYINGVHTFDKPQPWLFPIFPWAAFAFAGLI
                     VGSFLLFDWARRNESRTLAIAGVSGVALMELGLWLDARPLRLYGTYDFWHTSPNFFLV
                     RAGIVLVILFLGYAWCKWGAGEWGFSPLIEMGKCSLLVYWVHIEFVYGGLSVVPRRAS
                     SIATATRGFLTIFVAMTLLAVARNRFPKWKPQMMALFRGAENG"
     gene            10507..11493
                     /locus_tag="VG272_05885"
     CDS             10507..11493
                     /locus_tag="VG272_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HWF13245.1"
                     /translation="MSGERQLSIWTALGLWATAVANASVYGMWHGYSGRTYVVTLCIL
                     AFFLAIQLLFAAGDFGERFARRTGTHGGVLIAIVPFLAYVIYLAGTNNFTWHRALLAA
                     AYSLTPVLIAISAGTGKPGTWQDYAAMLAIFFPLKMHFLNALWPYPGPPISYIATMLL
                     AISVALGTFLFVRQFDGVGYNIVWGRDAALTISKNFGLIAVLVIPLGIYLDFIQFDMH
                     QAHWKSLPWDAISIFLLTAWPEELLFRGLLQNSLSRTFSNETVGWFTASAIFGLAHIT
                     NNGVFPNWRYALLAAIAGIFYGRTWRKTNSIFTAAVVHGLVDTVWHLAFRTL"
     gene            complement(11594..12370)
                     /locus_tag="VG272_05890"
     CDS             complement(11594..12370)
                     /locus_tag="VG272_05890"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HWF13246.1"
                     /translation="MSDELVIAGRAFRSRLIVGTGKYRSHQEMGRAHEASGADMVTVA
                     VRRVNLTDKTKESLLDYIDREKIFILPNTAGCYNADEAVRTARLGREVGLSNWVKLEV
                     IGDQQTLFPDTEELVRATQILVKEGFVVLPYTNDDLVVARKLIDAGAAAVMPLGAPIG
                     SGMGIQNHANLRILRERITEVPMIVDAGVGTASDAAVAMELGADGVLMNTAIAEAQDA
                     VIMAEAMREAVSAGRKAFLAGRMPKKLYAWASSPLSGVVR"
     gene            complement(12600..14411)
                     /locus_tag="VG272_05895"
     CDS             complement(12600..14411)
                     /locus_tag="VG272_05895"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HWF13247.1"
                     /translation="MFCFNMVYPTLTEKLLKAVDQLPQSRAVSLRVNGQWEPLPSSEF
                     LARIAGLSNALDQLGVKAGDRVGLFAPNRPEWHISDFAILGLGAADVPIYFNESPDRI
                     VYILNHSGAEVVIVAGEMQSRRLLECRDRLTSVKHIICAAAPADLDDDILRFETLVTA
                     TGEALVAEYRQRSARVTPDDVATIIYTSGTTGEPKGVMLTHNNLSSNEETSVAPYEMS
                     PADVAVSFLPLSHVYERVTAYAYLFHGVQVSYVERMEDLPQALLEVHPTLAAAVPRVF
                     EKLYANIVQKGHEATGLKRIIFNWAMRVAHDSVPWKAYGKDASYILNYRWRFADRIVY
                     SKIREGIGGRFRAFISGGGPLSREIAEFFWAVGVPVYQGYGLTETSPVVSANYPGTNK
                     VGTVGKPIENVHVRIAEDGEILVQGPCVMKGYYERPDDTKAVMTADGWLSTGDIGQID
                     EQGYLYVTDRKKDLFKTAAGKFVAPQPIENLLKAIPLISNAVLVGDARRFIAALIVPN
                     FANVESAARTEGRIFASYEEIAADPWVHGLIEKEVARMNAKLAQYETIKKFALLDHDF
                     TFDGGQLTYTLKLKRRVIAERYNQIIESLYADAPRVS"
     gene            14706..16052
                     /gene="ftcD"
                     /locus_tag="VG272_05900"
     CDS             14706..16052
                     /gene="ftcD"
                     /locus_tag="VG272_05900"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HWF13248.1"
                     /translation="MALMNRLIECVPNFSEGRDLAKVDEIVRAMSGVAGAWILDRTSD
                     ADHNRSVVTLAGTPEAVAEAAIRGAGKAAERIDMTSHKGVHPRIGATDVVPFVPLENV
                     SMEECVTLARRAGQELWDRYRIPVYFYEAAATRPERVNLENVRKGQFEGLLKDTMRDP
                     DRSPDIGEPRLHPTAGITAVGARKVLIAYNIYLSTSNVSVAREIARVIRFSNGGLRSV
                     KAIGVEMKTRNLAQVSINLTDYEQTPLHHVFDAVREEAGRRGCSVVGSEIIGLIPRKA
                     LELSEGHDLQLENFSPERILENRLAAAAGILPLPEEIMASSKNALEPLAESLREAVQS
                     FSRRALALSQVARRDSQPNTNCDAEGEAESYLELARAAAEIYERLGQLESASVPPIQL
                     DWIRVKQAAQGAARSALKSAEAILPSLQDAAMLRRFQTNSAEIEARLSGKTVATEK"
     gene            16115..17119
                     /locus_tag="VG272_05905"
     CDS             16115..17119
                     /locus_tag="VG272_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13249.1"
                     /translation="MKTRKTIWIATLIAIMSVLPGVARAGSVGSDILTMFPKESGELA
                     YANLKEARAFPWFNQLKEQMLPAKFREFETFLSSAGIDPNTQVTELAWALVPSKSSTD
                     NAAGAVPTMDQIVGVALGQFNPASAEAYFTSKKLPIVKVRTFSLFAFGGGSGPNDLFF
                     FFIDSNTAAFGQRQQLEKMIGIRYGEGQGLYANTDFSALVDQANGSGTVWAVLSPAYT
                     RLAMRQLVPETAQFPAAAQLTSKLKAMTISIKGSSGVDAKFEAVCGSTDDANTFAALL
                     TAGLMYRKYQVGNSNPELGALLDSAQVTPAGDRLDVKLSLTDDQMQSLIKRNTFVVSM
                     "
     gene            complement(17200..17568)
                     /locus_tag="VG272_05910"
     CDS             complement(17200..17568)
                     /locus_tag="VG272_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13250.1"
                     /translation="MANTGARKSKKLEQEEEQNELNKVSRTSEEIALDHQLEEEEGSA
                     ERQRLSGGRTGRQGVNTETDLEAGEDQNDELRHSNEQIVQQDLNQGMDTGTHDSTRHG
                     VDWGKSYQAPAKPRKARKKS"
     gene            complement(17849..>18593)
                     /locus_tag="VG272_05915"
     CDS             complement(17849..>18593)
                     /locus_tag="VG272_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HWF13251.1"
                     /translation="LTGRGVRGRVEGHEVALGNQKLLEELHSSAGPLAAKAEAARADG
                     QTVMFVLIDSKIAGMIGVADPIKETTLEAIGKLHADGIRIVMLTGDSRTTADFVAQKL
                     QIDEVVAEVLPQEKADVVKRFQTDGRFVAMAGDGINDAPALAQAQVGIAMGTGTDVAM
                     ESAGVTLIKGDLRGIVRARALSRATMSNIKQNLFFAFVYNSAGVPIAAGILYPFFGLL
                     LSPMIAAAAMSFSSVSVISNALRLRRTRL"
BASE COUNT         4316 a         5049 c         4883 g         4345 t
ORIGIN      
        1 ccgaatacgg gatcgatcgt tacgcgcctc gatcatcaca aatggagcga tgcgggatgg
       61 atggactggc gatcgaggaa cgattggctg tcttctccga tttcggttta tgaaatacat
      121 ctcggctcgt ggcggcgcaa tacccaggat acctggcggt ggctttcgta tggcgagctt
      181 gcggatcagc tgattccata tttaaagcag ctgaattata cgcacgtgga actgatgccg
      241 gtgatggaac atcccttcga cggatcatgg ggatatcaga cgctgggata ttttgccgca
      301 accagtcgct acggcacgcc cgcggatttc atggctttcg tggacagatt tcatcaagct
      361 ggaattggcg ttttgctgga ttggacgccg gcgcacttcc ccagcgatgg ccatggcctc
      421 ggcgaattcg acggcacgca tttgtatgaa catgcggacc cgcgccaggg gcgccatccg
      481 gattggggaa cgcttgtttt taattacggg cggaatgaag ttcagaatta tctgatttcc
      541 aatgcgcttt tctggatcga taaatatcac attgacgggc tgcgtgtgga cgccgtggct
      601 tccatgctgt acctggatta ctcgcgcaaa gaaggcgaat gggttcccaa cgaattcggc
      661 gggcgggaaa atctcgcggc cattgcgttc atcaaaagat tgaacgaagt gatttacgcg
      721 cggcatccgg gcgtcctgac catcgcggaa gaatccacat cctggcccat ggtttcgcgc
      781 cccacttacc ttggcggatt gggattcagc ttgaaatgga atatgggctg gatgaacgac
      841 acgttaagtt acttcgccgt ggatccaatc tataggaagt atcatcatag ccggatgact
      901 ttttcgatgc tatacgcctt cactgaaaat tttgcgttgc ccatctcgca cgacgaagtt
      961 gttcacggca aagggtcctt gattaataaa atgcccggcg atctttggca gcaggtggcg
     1021 aacatgcgcc tgttttttgc ctacatgatt gcgcacccgg gcaagaaact tatgtttatg
     1081 ggcagtgaat ttggccaacg aagcgaatgg aatcctgacg cgagtctcga atggttcctg
     1141 ctggagtacg atacgcaccg cggtcttcag acgctgacgg cggatctgaa tagtatctac
     1201 cggaacgagc gttctcttca cgaggtggat ttcgactggc acggatttga atggatcgat
     1261 tgcaacgatg cggattccag cgtgctatca ttcatccgac gagcaaaaga tcccgccgat
     1321 ttcatgatcg tggttgctaa ctttacgccg gttgcgcggg aaacttaccg ggtgggcgtg
     1381 ccggagccgg ggttctatcg agagatcatg aacaccgacg ccgagaaata tgccggaaca
     1441 aatatgggta atctgggcgg cgtgcatgct gaggcgattc cctggaacaa tcatccttat
     1501 tccattcaca tgcgcatgcc tgcgcttgcg gtcatttatt ttaagattca gcggtggtag
     1561 attcggattt taaattattt ttttaggatg ccgcctgaag gcgggcgcta cactactcgt
     1621 cggcttcggc agagtaatcg cggcgcggcg ggacaaaata tgcttcggct tggatagagg
     1681 tttccagcca acctcggcgc ctcacaattc cacgcacatt ttcaaccatt tccgggtggc
     1741 cgcataggta gatcttcgta tcgtcgggcg ataaattcca catatcggaa tacttgcgaa
     1801 tcaggtcatc cacgcgacct gtttcgccgt gccaatttgt atcttcccac gggcggctga
     1861 ccgttgaaac gtaggttaac cagggagctt cggcagccac attttcgatt tctttggaat
     1921 atccaagttc ccaggaacgg cttgcgccat caagaatgaa taatttgtga tgccccgcaa
     1981 attttccgga cttccaatcg caataaagtg aacggacata acttacgtag ggggcgacac
     2041 ctgtgactgt ggaaaccaga aaatggtttg tgcattgccc ggaaacgtcc aacgtgaaat
     2101 tccctttcgc cgactttcgc aagctaattt catcgcccgc gcgcaacgcg tggaagcgcg
     2161 gcgtcacctg gccgtgaggg accagttcaa aaaagaattc gagagttttt tcatggggag
     2221 cggaaacgat tgaataaggc cgctcatgat gtttttcggg agtgacgacg ccgagagtgg
     2281 cgtactggcc cgatcgataa gggaattccc cgccggcgtc tactcggatg gcccaaaggt
     2341 ccggcgaaat ctcgcgacgt tccaatatgc gtgcagcaaa gaatttactt ccggctggag
     2401 gcatccaaac tcccgagtga acaatttagt aagaaaaaga aatggatcgc gggcgaactc
     2461 gcatccatcg aaaccagcgc tgtttatacc cctcaaacgg gcaatatggc gcacagcgct
     2521 ctgttacaaa tctgatttta taaaatgaag cgttttactg cccttgctgg cgattgcgca
     2581 tcatttgcat tggatcgaat ttcttcggcg taccggtgat gtgatctttg gcgtcggtaa
     2641 tcactttcgt taacaccatc gtctcgcctt tattcgctcc attgttaaca cccttcacca
     2701 gcccccaagg ccccactttg tccgagaccc atgcctcacc ggaaccgtct ttcatgcgat
     2761 agtgctggca cgtaaaagtg cctgcgggaa ccgttaccga ttctgttccg accacttctg
     2821 ctttatcgtt aatatcgctc ggagtagatt gttgcatgcg gcgcgcctgg gcggccatat
     2881 ttccatccat ttccatagga tcttgtccgg gcatctgcat gatcatgcga gaagagacta
     2941 cgttattttc gttcacggtt gtcaaagatt tgacgtacac cggtgtggac gtccgagggt
     3001 ccaccattga catttccatc caataggccg gctttccatc gacatcttct ttccccacaa
     3061 tcgtaatttc cattggcatt ttcgttccat caggtttcgc gatctcgtaa gaagcgccgg
     3121 tgcccactac ggggcgccag actccgctta gagtcggtat gcgtccaccc atgcccattc
     3181 ccatttgtgc cgcagccgga atcgcgattg caagcgtcgc gaacaaaatt accgccaatc
     3241 gtttcaattt cattttattt gcccctttcc atcattcggt tctcatgcga agaatagccg
     3301 gctggattcc gagtagggat gaatttgcat ttttggttga tcctgctctg agggtccgac
     3361 caatcttgta tcagaactgg ccagtcagta gcaaatagat tagtgattct aatttggaat
     3421 tcagttattg gaaatttact gggtgggaag ccagcgaaat gaggtcttac tcgtaggcga
     3481 gagcatccac aggatcactt tgcgcggctc gcagcgccgg gtatgcggcg cccgcgatgg
     3541 ctccgccgat agtcagaaga attgcgcgga gaatccagcc gccctcgatg gcgatctgaa
     3601 gtgtaggaga cgttttatgc aaaatagcca caatgctgta cgtacaaatc aaaccgaaaa
     3661 tcgagcccag ggcaaccatc acgagagtct ctcccaggat caatccgcaa acttccatgc
     3721 gggaggaccc cagcgcgcgt agaataccga tgtcgcgcgt gcgctcaaaa accagcgtgt
     3781 gcatcgtgag cagaacaacg aggaaactga tcaccacgcc aagaatgacg aaagcgcgaa
     3841 taaatggctt gagctccggc aagttggaag aagtcatcaa cgtgctgaac tccgccatgg
     3901 atcgaatttt gaaagtggga agcagtttta cgatttcgct tctcgtgccg tccgtattgc
     3961 cagtgctgcg aacgaaaaac atcgaaacgc ggccctccgc tcccacaata tcctgcgcgg
     4021 tcttgattgg aataaaatag cgcgcgcctt tgccgtgcac gacaatgccg gaaattgtga
     4081 attcgtggcc aaggagcgtt actttttgcc ccacacgcaa cttacgcgat ccggcagcaa
     4141 ggtcatcgac gatggtctgg tccggcgcct gaaaaggccc gccggaaaga aatgtgaaac
     4201 catcgcttag gccggcaaac tgctggtaat caatgccgta gagcactcca agattttttt
     4261 ggtcgactgc gatcagaacg ggcgccacga cccggacccc gggtaatgcg ctcatctgcg
     4321 cgcccagagt ttcaggcatg acggcgcttg aaaatgcaaa aaagatggat gaattcggag
     4381 gctgaacaat aatatccgcg ccgacgccct cggcgcgggt gcgccaatcc gaaagcgcgc
     4441 ccgaggtaag cccaacaatc agtaatacga ggaaaacttg tagtgcgagg gcaaatacgc
     4501 ttagcgcgga gcgcagcggg tgcacgccca gattactcca cagcaaatcc cagatcacaa
     4561 atcctttata ttgtttttca ggcatccgat ggttccgcga gaagttcccc ggccagtaat
     4621 ctaacatcac gcaacactgc ggtcaaagag acgcgaaccg tgggccgggg aaagttttca
     4681 acagttgtgt tgaaaacttt gtggaaaaga ggtatcgaat tcgcgtacct cgtgaaaaac
     4741 aatgaccttg atgcggattg cacaaagatg aagcatattt ttttcgccga caaaaaataa
     4801 tttgtagaaa tttcgcttca ataccattta ggcgggcgca caagagcgac gtttatggtt
     4861 tgttcggttt ggttgatgtc gtaatactct ccgtaggtct tccagccttc tgcgatcacc
     4921 tgcacgagaa attttccggg cggaatcacg ggaacatggc aaataccgga ctggttcgtc
     4981 ttcaaattca tttcgatttt cttgtcttta ccgagtttac gcccctcgac atagcggaca
     5041 tagacactgg cgctttcaat cggcttcttg tcctctccac cggttactac aatggtcagt
     5101 cgcgagtcct tcgacccgct gtccgcttta ccattcgtgt ctccggactt gtcctgagca
     5161 tttgccgcaa ccatgagaat tagaaacagg aatggcaata ctcgcagcga agcctttacg
     5221 caattttttc ccaagttcat ctttggcctc tgactttatt tcttagagga aacattgatg
     5281 gccccatttt tctctttttt atgggattac gcaagtagaa cgggcgaccg gaagcggatc
     5341 catcggaatt gctcgggcaa aaacgacaag cacgaaaaga agagcggcct accgacacaa
     5401 cagccaagca atccggtttt gtagaggctt ccccgtccgt cggtcttcgc ggcggagagg
     5461 ccgacttgac catataaaat gacatgcttc cgcttccctt tcgcccgtcg cattgactca
     5521 accccggtca tgagctactt tcgattcgcg aagaactcca aggagatttt tttcatggga
     5581 tttgcaaccg acgcgattca tgtggggcag gaacccgatc caacaaccgg cgcaatcgta
     5641 gcgcctattt accagacttc aacctatatc caggaagaat tgggaaaaac aaaaggctac
     5701 gactacgcgc ggacgaatca tccgaaccgc aaggcgctgg aacggaccgt ggcgaagctg
     5761 gagggtggca gcgcggcgta tgtctttact tccggaatgg ccgccattga ctccgtgttt
     5821 cggttgatgc gtcccggcga ccacgcaatt atttccgagg ccgtctacgg tggcgtgatt
     5881 cgcctcacaa cgcaatttct aatccaatgg gggctggagt tcagttttgt ggatacttct
     5941 tcgccggagc tcgtcgagcg cgcgataaag cccaatacca aaatgattta cgtggagacg
     6001 cccacaaatc caacgatgtg cgtcacggac attgcagcca tcgccaaaat tgccacagac
     6061 cacagcgcca ccatggtagt ggacaacacg ttcctcagcc cttatctgca atcgccgctg
     6121 gcgttgggcg cacatattgt tgtccattcg atgaccaaat atttaaatgg gcacagcgat
     6181 gccgtcggcg gatccgtcat cttgacgcgt gcggaagacg cggagaaaat ttatttcctg
     6241 cagcgttcga cgggagctgg gctagcgccc atggattgct ttctcgtgtc gcgcggaatc
     6301 aagacgctgg cagtgcgcat gaagcagcac gatgccaatg ggcgaacggt tgcccgattc
     6361 ctggaagacc acccgcgcgt aaaatgcgtt ctctaccccg gcttgccttc gcacccacag
     6421 cacgatgtgg ctgtgcggca gcagcgcgga tttggagcca tgatttcgtt tgacgtggtt
     6481 acgatggaga atgcgcgcac ggtcctcaac agtttaaagg tgtgttcgct tgcggagagt
     6541 cttggcggag tggaaacgct aatctcgcac cctgcgacca tgactcattc ttccgtgcct
     6601 gccgaagtgc ggcaacgaat tggaattaca gatggcctga ttcgcatctc cgtgggcatc
     6661 gaagatgtgg aagatttgat caacgatcta gatcaggcgc taaggcagat ttgagtagga
     6721 taggcttcac agtttgcgga aaaaatagtt tattctgtca ttccgagcga agcgaggaat
     6781 ctctcttcga tttaagtata agaaaataaa gaaggaagga ttcctcgctt cgctcggaat
     6841 gacaaaatgt tgagtttttc cgcagcctct tcagcctgtc cggtttaaat ttctgtaagg
     6901 agcaaaagtc aaaagaaaac aggctgaagc ctgttctact cacacctcat ccatccatcg
     6961 caggtatttg cgcgaaccgg tggcgatggg cagcgccaga aactccggca cgtcgtagga
     7021 gtggaggcgc tcgacagctg cttggagctt cgtaaggtgc ttcctcgacg ttttgatcag
     7081 caacaagcgc tcccgcgccg tttctacctt tcccttccag cgataaatcg agctgactgt
     7141 gccgggaaga atatttacgc acgcggccag ccgagcttcg acaacagcgc acgcgatgcg
     7201 tttcgcctcc gccgtgcttc cgcacgtgac caacaccacc aatttatcgg tcaagatttc
     7261 ctccgaaccc gttactcttt ctcgcaaacg tcatttccat ctttctcata tttgcacgaa
     7321 aggctacaat acctgacgga aggcgaaaat aattttctaa ccaatgacgg cgaccatcaa
     7381 atttggaact tcaggctggc gcgcaattat cgcggatgaa tttacattcg cgaatgttcg
     7441 ccttgcaacc gccgccattg cggaacacgt tcgcagccgc aatcccaagg caacattgat
     7501 cgtcggacat gacacccgct tctttgccga agaattcacg catacggcgg cggaaattct
     7561 tcgcgagtac ggcattcgcg tgctgttgtg cgacgatcca actcccactc ctgttatcgc
     7621 ccatgaaatt attcacagga aagtagatgg cgccatcaat ttcacggcga gccacaatcc
     7681 cgcggagtat caaggcctga aattttccgg gccggacggc ggtcccgcgc tcccggaagt
     7741 gacacacgat atcgaagcgc gcgccgcaaa gattggcgat cgggctcccg cgaaaccgaa
     7801 cgacgatcca tttgagaaaa ttaatccgcg cagcgcttac cttgagcggc tgacgcaact
     7861 tgtgaatttc aaagcgctgg gcgatgcgaa actgaacatg gtcacggacg cactgcatgg
     7921 atgtggagca ggttatctgg atgaagcgct ttcctcacac ggcgtgaaag tgacggcgct
     7981 gcgcacgcat cgggatgtgc tgttcgatgg ctcgggaccg gatgtatccg aggacaatct
     8041 gcaaaaaact cgagccgcag tgctcgcgaa taaagctgcc gcaggactcg cggccgatgg
     8101 cgatgccgat cgcttcggca ttctggattc agatggcaca tggatgcagc caaatcatat
     8161 cctcggctta ctgtacgatt acatcgtaga aacccgcgga tggaaaatgc ccgcagcgcg
     8221 tagcgttgcc acttcgcact tgatggacgc ggtggcgcgg ctgcacggaa ccaccgtcat
     8281 tcaaacgccc gtggggttta aatacatcgg cgagctgatt caagaagaca aaattgcgat
     8341 gggtggcgaa gaaagcgccg ggatgaccat acgcggccac cttcctgaaa aggacggcat
     8401 tctcgcgtgc cttctggtcg ctgaaatgat tgcagtgagg cgaacttcct tgtccgaaca
     8461 attaaagtct ctgtttaagc gggtggggtc ggagttctgg ccgcaacgca ccaatctgca
     8521 cttgccggac gacgtcaaaa ttagaaccgt aaacaggctg aagacggact tcaaaacatt
     8581 tttggggcgg cccgtgaaaa gcctggaccg caccgacggt ttgaaaatgg aatttgaaga
     8641 cggatcgtgg gtccttttgc ggctttccgg cacggaaccg ttgatgcgcg tttacaccga
     8701 agccgcgacg ttgaaagaat ctttacaaat tgcgagcgag acggaagcct gggtgaacag
     8761 ctcttcgcag gaagcaaagg catgatgacg ctaaaggaaa ttcgagaaat ttctccgagt
     8821 gaagtgattg cgtacgcgcg caaaaacacg cggcagattc tggcgctcgc gctgttcgcg
     8881 ctgtttgttc atgacatttt tggtccgcac ggattcatcg ccatgcgccg cacgcaaaag
     8941 gaaatcgatc agattcacga gcaaatcgtc aaaatgaata acgagaataa agcgttgacg
     9001 gaccaggtct cttctctgaa gtccgacccc aaatccatcg agcgcattgc ccgcgaggaa
     9061 atgggcctgg cacggcccgg tgaaatgatt tttaaaatcc ccgacttcgc gaagccgccc
     9121 gccaagtagc agaggcttca caaccttcga agagatcatc taagctgtca ttccgagcgg
     9181 agcgaggaat ctctcttttt tcctcataat caattagcca aatcgaagag agattcctcg
     9241 ctccgctcgg aatgacggaa gttgagatgt tccactgcct gttcagcgtg tgctgcttga
     9301 gttcagccat tctcagcgcc ccggaatagc gccatcattt gcggcttcca tttgggaaac
     9361 cggttacgcg ccacagcaag cagcgtcatg gccacaaaaa ttgtgaggaa cccgcgtgtt
     9421 gccgtcgcga tgctggaggc gcgccgtggc accaccgaaa gtccaccgta aacaaattcg
     9481 atgtgcaccc aatatacgag cagcgagcac ttgcccattt cgatcagcgg gctaaacccc
     9541 cactcccctg ctccccattt gcaccaggcg tagcccaaaa ataaaatgac aagcacaatt
     9601 ccagcgcgga ccaggaaaaa attgggactc gtgtgccaaa aatcgtaagt gccgtagaga
     9661 cgcagagggc gcgcgtccaa ccacaaccca agttccatga gcgcgactcc gctcacgcca
     9721 gcgatggcta atgtgcgcga ttcattcctt cgcgcccagt cgaacagcaa aaatgagccg
     9781 acgatcaagc ctgcgaaagc aaatgcggcc cacggaaaaa tcggaaacag ccatggctgc
     9841 ggcttatcaa acgtgtggac tccgttgatg taagactcca gccaccaggc gagcgagtgc
     9901 ggccgccaac tggtccacaa aggcggagta agcatggcga tcgctgccgc gcaaacgatt
     9961 gccgtcaaga tcgtttttcg ccgcaactgc gacgcgtcgg tcgcacctcc agcagccgca
    10021 atgcggcatg cgacgcccaa catcatgagg gaaataccga tcacattcag gatatcgacg
    10081 cgcaaaaggt cagtccatga cgcccacggc tggccgagca aaaattcctg taaacgaaaa
    10141 agcaacccaa gaagaaatat ttctccaccg cgcaggatcg tcgtgcgtgc cacgtcatcg
    10201 gctgacacgc ccttttggcg cagtttctcc gtgaccaacg cgaaggaggt gccggcaaga
    10261 aataaaaaca tcggcgccgg cagcgtgccg ccaagctggg acatcttgaa gaagccgctg
    10321 tcacgagccg caccgcccag ccaggaatcg taacaatgcg tttgaaacat cacgaggcat
    10381 gcaaagcccc ggagccagtc aatgtaagca attctttttg tgatcgtgtc agccatggat
    10441 aattaatttg cttgagtggg ctccttgcgg cccgtgcttt cggcgagtga gatacactat
    10501 cgtgccatgt ctggtgaacg ccagctttcc atttggactg cccttggttt atgggccact
    10561 gccgttgcaa acgcaagcgt gtatggcatg tggcacggct atagtggccg cacttacgtc
    10621 gtcacattat gcatcctggc attttttctg gcgatccaac ttttatttgc cgcaggtgat
    10681 ttcggcgaac ggtttgcgcg ccgcacaggg acgcacggcg gcgtcctgat tgctattgtg
    10741 ccttttcttg cgtacgtgat ctatctcgcg ggaacaaata attttacctg gcatcgtgcc
    10801 ttgcttgccg ccgcgtattc gctgacgcca gttttgatcg caatttccgc gggcacgggt
    10861 aagccgggca catggcagga ctacgccgcg atgctcgcga ttttcttccc attaaaaatg
    10921 cattttctca atgcgctgtg gccttatccc ggcccaccca tcagctacat cgccacaatg
    10981 ctgctggcga tcagcgtggc gctcggaaca tttcttttcg tgcgccaatt tgacggcgtg
    11041 ggctacaaca tcgtgtgggg acgagacgcc gcacttacga tatcgaaaaa cttcgggcta
    11101 attgctgtgc tagtgattcc gctgggaatc tatctggatt ttattcaatt cgatatgcat
    11161 caggcacact ggaaatcact gccgtgggac gcgatttcca tttttttact gaccgcgtgg
    11221 cctgaagagt tattgtttcg cgggctactg cagaattccc tgtcgcgtac attttcaaat
    11281 gagactgtgg gatggtttac cgcatccgcg attttcggcc tcgcacacat caccaacaac
    11341 ggcgtctttc caaattggcg ctatgctctg ctcgcggcaa ttgccggaat tttttatggg
    11401 cgcacatggc gcaaaacgaa cagcattttt acggcagccg ttgtccatgg cctggtcgat
    11461 accgtttggc atctggcatt ccgaactttg tagcagcttt aagtggcgcg ggcttcagcc
    11521 tgcggggttt cggccctgca gggtcaaaat tccgcaggct gaagcccgcg tcactaaata
    11581 caagatcgca tttttatcga actacgccgg acagcgggct cgacgcccag gcgtaaagtt
    11641 tcttcggcat gcgtcctgcc aggaacgctt tgcggcccgc ggacacagct tcgcgcatcg
    11701 cttccgccat gatcaccgcg tcttgcgctt ccgcgatcgc cgtgttcatc agcacgccat
    11761 ccgcgccgag ttccatcgca acagcggcat ctgatgctgt gccgactccg gcgtccacaa
    11821 tcatcggcac ttcggtaatg cgttcgcgca aaatccggag attggcgtgg ttctgaattc
    11881 ccatgccgga gccgatgggc gcgccgagcg gcatcacggc cgctgcgcca gcgtcaatca
    11941 acttgcgcgc gacaaccaga tcatcgttcg tgtaaggcaa cacgacgaat ccttctttca
    12001 ccaggatttg cgtcgcccga accaattctt cggtgtccgg gaataatgtc tgctgatcgc
    12061 caatcacttc cagctttacc caattggaga ggccgacttc gcgccccagc cgcgcggtgc
    12121 ggacggcttc gtcggcgttg tagcatcccg ctgtgttggg caaaataaaa atcttttcgc
    12181 ggtcgatata atcgagcagc gattccttcg tcttgtccgt caagttcact cgacggactg
    12241 ctacggtaac catatccgcg ccagacgcct cgtgcgcgcg ccccatctcc tgatggctgc
    12301 ggtattttcc tgtgccaacg atcaggcgcg aacggaatgc gcgccctgct atgaccaatt
    12361 catcgctcat ttgcttctcc tgaacatttt ggaacttggc ttattgtaac gctccgcgcg
    12421 actggtgcac aggcttcggt cagcggcgct tggttggcgc tggctcggcc taacaccaat
    12481 tgctactttg acaaacgcga caggctaacg gcgtatgtaa aacgtcgcac ttcaatctgt
    12541 agcgcccgtc ttcaggcggg catgagacta aacccacagt gcccgcctga aggcgggcgc
    12601 tacgagaccc gcggcgcgtc ggcgtataag ctttcgatga tctggttgta gcgctcggca
    12661 atcacgcggc gtttcagctt tagcgtgtac gtaagttgtc cgccatcgaa cgtaaaatcg
    12721 tggtccagaa gcgcgaattt cttgatcgtt tcatactgcg caagctttgc attcatccgc
    12781 gccacttctt tttcgatcaa tccatggacc catgggtcgg cagcgatttc ctcatacgag
    12841 gcaaaaatgc ggccctccgt gcgagcggcg gactccacat tcgcgaaatt tggcacgatc
    12901 aaggctgcaa taaatcttcg agcgtctccc accagtacgg cattcgagat cagagggatc
    12961 gctttcagta ggttttcaat tggttgcggc gcgacaaatt ttcctgccgc cgttttgaac
    13021 aaatcctttt tgcggtccgt gacatacaaa tatccctgct catcaatttg tccaatgtcg
    13081 cccgtcgata gccaaccatc ggctgtcatc acggcttttg tgtcgtcagg cctttcgtaa
    13141 taacccttca tcacgcatgg tccctgcacc agaatctcgc cgtcttccgc gatgcgcacg
    13201 tgtacatttt caatcggctt ccccaccgtg cccactttat ttgtacccgg atagtttgca
    13261 ctcacgacgg gtgaagtctc ggtcaggcca tacccttgat atacgggcac gcccacggcc
    13321 cagaaaaatt cagcgatttc acgggaaagc gggcctccac cggaaatgaa cgcgcggaag
    13381 cggccgccaa tcccttcgcg gattttcgag tacacaatcc ggtccgcgaa cctccagcga
    13441 taattcaaaa tgtaagaagc atccttgccg tacgctttcc agggtactga gtcatgggca
    13501 acccgcatgg cccagttgaa aataatacgt ttcaaccctg tggcctcatg gcctttttgt
    13561 acgatattgg cgtagagctt ttcaaaaacg cgcgggacgg cggcggccag cgtgggatga
    13621 acttccagaa gcgcctgagg taaatcctcc attcgctcga cgtaagagac ctgcactcca
    13681 tgaaacaaat atgcatacgc ggttacgcgc tcatacacat gcgagagcgg cagaaagctg
    13741 accgccacat ccgcggggct catttcatag ggcgcgacgg aagtttcctc attcgaactg
    13801 aggttgttgt gcgtgagcat cacgcccttg ggctcgcccg tggtgccgga ggtatagatg
    13861 atcgtggcaa catcgtcagg agtgactcgc gcggatcgct gacgatattc cgccactaat
    13921 gcctcacccg tggctgtcac cagtgtttcg aaacggagaa tatcgtcgtc caaatcagcg
    13981 ggcgcggcgg cgcagataat atgcttcacc gatgttagcc ggtcgcggca ttccaacaag
    14041 cggcgtgact gcatttcgcc ggcaacgatt acaacttcgg ctccggaatg attcaagata
    14101 tagacgatgc ggtcgggcga ttcattgaaa tagatcggga cgtcggccgc acccagcccg
    14161 aggatggcaa aatccgaaat gtgccattcc ggtcgattgg gtgcgaagag cccaacacga
    14221 tccccagctt tcacccctag ctggtccagc gcgttggaaa gtccggcaat gcgtgccaaa
    14281 aattcagagg aaggcaacgg ttcccattgt ccattcacgc gcagggatac cgctcggctc
    14341 tgcggcaatt gatccaccgc tttcagtagc ttctcggtaa gtgtggggta aaccatattg
    14401 aaacaaaaca ttaccgcacg attctctata cgcgcaatgg aacttcattt cattcttcaa
    14461 attttgaaat cagcttccgg cgcgggccat ggaatgacta ttcacgcttg attctcgaat
    14521 tcgactgccc aaacgtccag ttggcaagtt agcgggattg ctcatcttcg aattgaggag
    14581 cagtctgaat atctcgtatc atggaggtgc agcccagata tgcaaccgag aagtgttacc
    14641 cagcttggaa accgtaccgc gcaaggttag acccactgca cagggagttg atcctgcgta
    14701 aatcaatggc ccttatgaac cgtttgattg aatgtgtgcc gaatttttcc gaaggccgtg
    14761 acctagccaa ggtggatgag atcgttcgcg ccatgagcgg cgtcgccgga gcctggatcc
    14821 tcgatcgcac gagcgatgct gaccacaacc gctccgtcgt tacgctggca gggacgccgg
    14881 aagccgtggc cgaagctgcc attcgcggcg ctggaaaagc agctgaacga attgacatga
    14941 cgagccataa gggagtgcat ccgcgcattg gcgcgaccga tgtggttccc ttcgttccac
    15001 ttgaaaatgt ttccatggaa gaatgcgtta cgttggcacg ccgagccgga caagagcttt
    15061 gggaccgcta ccgcattccc gtttattttt atgaggctgc ggcgacccgg cctgaacgcg
    15121 tgaacctgga aaacgttcgc aagggacaat tcgaggggct actgaaagac acgatgcgcg
    15181 acccggatcg ttcaccggac attggcgaac cgcgcttgca tcctactgcc ggaattacag
    15241 cggtgggcgc gcgaaaagtt ttaatcgcat ataatatcta tctgagtacg agcaacgtat
    15301 cggttgcccg ggagatcgcg cgcgtgatcc gcttttccaa tggcggcttg cgcagtgtaa
    15361 aagccatagg cgttgaaatg aaaacgcgaa acctcgcgca agtttccatt aacctcacgg
    15421 attatgaaca gacaccgctg caccatgtgt tcgatgcggt cagggaagaa gccggacgcc
    15481 gcggctgttc ggtggttgga agcgaaatta ttggcttgat tccgcgcaag gcccttgaac
    15541 tttccgaggg ccatgatctg caactggaaa atttttctcc cgagcgaatt ttggaaaatc
    15601 gattggccgc agccgctgga attctgccat tgccggaaga aattatggca tcttccaaga
    15661 atgccctcga accgttggcc gaatccttgc gcgaagccgt tcaatcattt tcccggcgcg
    15721 ctttggcctt gtctcaagtt gcgcggcggg actcccagcc gaatacgaat tgcgatgcgg
    15781 aaggcgaagc cgaatcttat ttggaactgg ccagggctgc ggccgaaata tatgagcggt
    15841 tgggacaact cgaatctgcg tccgtgcctc caattcaact ggactggatc agagtaaagc
    15901 aagcggccca gggcgccgct cgtagtgcct tgaaaagcgc ggaggcgata ctcccctcgt
    15961 tacaagatgc ggccatgctg cgaagatttc agaccaattc agcggaaatt gaagcaagat
    16021 tatcaggcaa gactgtcgcc accgaaaaat gagttctctc agaaactaaa tagaagcttg
    16081 cgtgttagtt tgaataggtt ggaggaagaa gaaaatgaag acccggaaaa ctatttggat
    16141 tgccactctt attgccatta tgtccgttct gccgggggtg gcccgcgccg gcagtgtcgg
    16201 gtctgacatt ctgaccatgt tccctaagga atctggcgaa ctcgcctacg ccaatctgaa
    16261 ggaagcgcgc gcatttccct ggttcaacca gctgaaagaa caaatgttgc cggcgaagtt
    16321 ccgcgaattc gaaacttttc tctcttcggc aggtatcgac ccaaatacgc aggtaacaga
    16381 actagcatgg gcgctggtcc ccagtaaatc gtccaccgat aacgctgccg gggccgtacc
    16441 gaccatggac cagatcgtgg gagtcgcgct cggtcagttt aatccggcct cggcggaggc
    16501 ttactttact tcgaagaagc tgccgattgt gaaagttcgc accttctctc ttttcgcgtt
    16561 tggcggtggg agtggaccca atgacctctt cttcttcttt atcgattcga acacggccgc
    16621 atttggacag cgccagcagc ttgaaaaaat gattggcatt cgatacggcg aaggccaggg
    16681 actatatgcc aacactgatt tttcagcttt ggtcgatcag gcgaatggca gcggaacggt
    16741 ctgggctgtt ttgagccctg cctacacacg cctagccatg cgacaactcg ttccggaaac
    16801 cgcgcagttc cctgcagcgg cgcagcttac ttccaagctc aaagccatga cgatcagtat
    16861 taaaggcagc agcggcgtgg atgcgaagtt cgaagcagtt tgcggatcga cggatgatgc
    16921 gaatactttt gctgcacttc tgaccgctgg gctcatgtac cgcaaatatc aagttggaaa
    16981 ctcgaacccc gaactgggcg ccctgcttga tagcgcgcaa gtgacgcccg ccggggatcg
    17041 tcttgatgtg aagctttcgc ttacggatga ccaaatgcag tcgctcatta agcgaaatac
    17101 attcgtcgta tcgatgtagc aagcacgcaa aaagccctaa ttcattcagt tcaagttaca
    17161 gaggtgcaat aattctgcat ctctgtattt ttgtgctggc tagctcttct tccgggcctt
    17221 tcgaggtttc gccggagcct gatacgactt cccccagtcc acaccatgcc gagtagaatc
    17281 atgggtgccc gtgtccatac cctgattcaa atcctgttga acaatctgct cgttggaatg
    17341 ccgcaattca tcattttgat cttcgcctgc ttccaaatcc gtctccgtgt tgacaccctg
    17401 ccgtccagtc cttccgcccg agagtctttg tcgttccgcc gatccctctt cctcttccaa
    17461 ctgatgatcc aaggcgattt cttcgctcgt gcggcttacc ttattcagct cgttctgctc
    17521 ttcttcctgc tctaattttt tggattttct tgctcctgtg ttggccatct cgccctcctt
    17581 gaaaactagg gaatcctgcc cagcttacga atggaaatat caactcccaa aattatcgga
    17641 gcgggcacga acggggacaa tcaggtcttc catggagggc atgtaaggta tcgagcattc
    17701 caattcagaa ccatagaatg caagatcttt gcctacagta acttacagca atcgatttcg
    17761 cgagcttcaa ctcaagcgcc ctgattcctt ttcgctccgg atcttttcat ttttacgcgc
    17821 ctgtctgctt tctgttgacg acctcgaatc acagtcttgt gcgcctaagt ctcagtgcgt
    17881 tgctgatcac cgacaccgaa ctgaagctca ttgcggcagc tgcgatcatt gggctgagca
    17941 gcaggccgaa gaatggatac agtatccctg cggcgattgg gacgcctgcc gagttatata
    18001 caaacgcgaa gaataaattt tgtttgatgt tgctcatggt ggcgcggcta agagcgcggg
    18061 cgcgcacgat tccgcgcagg tcacctttga tcagcgtgac cccggcgctt tccatggcaa
    18121 cgtcggttcc cgtgcccatg gcgattccga cttgcgcctg ggccaaagca ggagcgtcat
    18181 tgatgccatc gccggccatg gcaacgaagc ggccgtccgt ttggaagcgt tttaccacgt
    18241 cggctttttc ctgcggtaaa acctctgcta cgacttcgtc gatttggagt ttctgggcta
    18301 cgaagtcggc cgtcgtgcgg ctgtcgccgg tgagcattac gatgcggatg ccgtcggcat
    18361 gaagtttgcc aattgcttct aaggtagtct ccttgatcgg atcggctacc ccgatcatgc
    18421 ccgcgatttt actgtcaata aggacaaaca ttacagtttg gccatccgcg cgtgctgctt
    18481 ctgcctttgc ggcgagcgga ccggcggaag agtgcaactc ttccagcagc ttttgatttc
    18541 ccaacgctac ctcgtggcct tcgacgcgtc cacgaactcc ccggcctgtg agc
//
LOCUS       DASZMB010000128        38899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_693154, whole genome shotgun sequence.
ACCESSION   DASZMB010000128 DASZMB010000000
VERSION     DASZMB010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 38899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38899
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693154"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /locus_tag="VG272_05920"
     CDS             complement(<1..1363)
                     /locus_tag="VG272_05920"
                     /inference="COORDINATES: protein motif:HMM:NF023169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HWF13252.1"
                     /translation="MRHKAAKIRPILRVFFASALVLCLFPLISQTIARADDNASSRRD
                     AANAQFDRAEKARQVLEARPESARTLKDYTALVIQYQRVYLTTSHAENVPASLNQVAE
                     LFRTMGDLFDAKYYQRSIKSFAFLLKEYPTSKYREDAQLAVAQIQQDDLHDAGAAQKS
                     YEDFLSAHPHSTHAAEVRASIDKLNAASAAAAKAAQPIPVSNTRSDRMSPKNISAEKV
                     PPTTDTKPGSPEESSNQNSSSPQVSRIRTWNADTYTRIVIDVGSKVKYQAARISGPDR
                     IYFDIEGAKINTALMRKPIDVESPFLKTVRVAQYQASVARVVLEINHVADFSVFLLPD
                     PYRLVVDVYGTSAAAEAAARETAPAAGPTAMDATTSKTDKMFAANTSAKPPVRLAEKT
                     AKSPFVPPPDAPVMQSPPKFNSATSAPSASASVSTSEATDTESSARKIPNTPMKAPQK
                     STAA"
     gene            complement(1372..2094)
                     /locus_tag="VG272_05925"
     CDS             complement(1372..2094)
                     /locus_tag="VG272_05925"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWF13253.1"
                     /translation="MRRLNKSVTIFLGALGLWAIPARGQIVSYTDVSGKRVFINAEPA
                     SVAHTSKAPLLKTAANGFTLRTAPPSFGVSSEVPAFDQANREKITEMIREVSARYHVD
                     PALVRAVIQTESNWNTNAVSRVGALGLMQLVPGTAQQLGVSNALDPKENLDGGVRYLH
                     SLLERYNSDLDKALAAYNAGPGAVDRAGGIPRYRETRNYVQKVTDSYYRPGSDRLPRA
                     FEQQRPIYRTVQSDGRVVFTNE"
     gene            complement(2210..5065)
                     /locus_tag="VG272_05930"
     CDS             complement(2210..5065)
                     /locus_tag="VG272_05930"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HWF13254.1"
                     /translation="MPSGNDIRTKFLEFFAERGHRIVRSSSLVPTNDPTLLFTNAGMN
                     QFKDVFLGLEQRDYTRATTSQKCVRAGGKHNDLENVGFTNRHHTFFEMLGNFSFGPVG
                     VVGDQFPYSKREAIAYAWELVTSPNWFNIPKEKLFITVFGGAEIFPGMELIEDSEARN
                     FWLEQKVALERIYAIPGLKENFWAMGDTGPCGPCSEIHYDMGAKASDQGHMDCKFPCD
                     CGRYVELWNLVFMEYNRQAGPDWAKVSDLPKHSIDTGAGLERLAAVLQGKISNFDTDL
                     FVPLIERAGELTGTRLGASQRSDASLRIIADHARATTFLISDGVIPSNEGRGYVLRKI
                     MRRGIRHGRLLGSAEPFMAEMSRTVTALMRDAYPDLNDSIEKVCKIILSEETRFAHTL
                     SEGLGRLEEDLELALLDVELPGPGNNLTRNEASRKYDYLTLGPRMAAIKLSGKKVVYK
                     GERAFKLYDTFGLPLDFIQDAVRDIGVEFDRTGFQQAMEAQRTRARASWKGGHKDVAN
                     PVYAKLAERFKTEPDFYFSTTAKNRRIEAIVTKDGLINELPAGAEGEIVLDRTVFYSE
                     SGGQVSDIGTLWNNEHTMQLGEVRGAYYPVSGLIAHRVIAKEKLAVGDLIAGEADAAR
                     REHIKRNHTGTHLMHAALRNILGTHVKQAGSLVEPARLRFDFSHFAAIDPADLRDIEQ
                     QVNEEILLNNVIQTNVTTIDDALASGALAFFGDRYPEQNVRVVTIPDANAPRGFYSKE
                     LCGGTHVTRTGDIGVFKIVVEQSAAAGVRRIEAITGAAALADYQRAQQTLRDLSARMA
                     TPDENLLAGIERMEQTQKQLEKQLETLKRKGALSKLDDLVARAHEIKGVKVISAQLEG
                     VDRETMRQVVDSLRQKLGSGVVVLGTIEDGKVALLAGITKDLIPKLHAGKIIQATAKE
                     VGGSGGGRPDLAEAGGKDTSALKSALDHVYQLIDQLL"
     gene            5758..6159
                     /locus_tag="VG272_05935"
     CDS             5758..6159
                     /locus_tag="VG272_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13255.1"
                     /translation="MDLKCANPICNSEYENGRGRLLRIQQTLSPEKPPSHWHGMKHYW
                     LCARCSDKFTIEIQKGTGVLLAEKLEEGAETQAAYTLLESETLPKQGVKPLLPRLTRS
                     RARHRKQKVDLTPTTASAADVLEIRKTERRG"
     gene            6165..6392
                     /locus_tag="VG272_05940"
     CDS             6165..6392
                     /locus_tag="VG272_05940"
                     /inference="COORDINATES: protein motif:HMM:NF022229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HWF13256.1"
                     /translation="MSEQIGTMAGAIWHALEANGEMTLSKLKKELNAGSPLFDWAIGW
                     LAREDKIMLTTEKRTTRVCLKGWHTQSMHAA"
     gene            6666..7502
                     /locus_tag="VG272_05945"
     CDS             6666..7502
                     /locus_tag="VG272_05945"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWF13257.1"
                     /translation="MNSRAAKCGVALCIFSLLFLNSLPIRAQEANASLSGAITAATGA
                     SVANAKVAVKNLSTGESRETQTDSNGHYTIPNLPPGDYEISVTAPEYSTNTLKVTLSA
                     AGPNNANVTLTGALSLSDLGFSPDQAQGTAADQARLNKRSHMLMLHQRIGLIAAIPMI
                     ASVATSFGAGGRGTSTTDRYLHLALGSATGDLYFTSAYFAIRAPSIPGTQRRGQIRVH
                     RALAWIHGPGMILTPILGAMAFDQKSKGEKVHGIASAHGAVAIVTAAAYGAAIASVSF
                     KF"
     gene            7535..8074
                     /locus_tag="VG272_05950"
     CDS             7535..8074
                     /locus_tag="VG272_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWF13258.1"
                     /translation="MIKKIICVFLFLALPGLAAADSQWVLVESTLTYHVSHPLHQTEG
                     VSHAAKGKGVCRGGQCDFLIAVPVKSFDSGDSNRDLHMLQVARGGEFPMITVRTHLPE
                     DASTLATIPVDLEVQFAGQTVPYKQIAFQHVVKGNDTFIAGTIPLTLTDFKIDPPSLL
                     AIPVKNEIPVRVEMKWRQM"
     gene            complement(8186..8710)
                     /locus_tag="VG272_05955"
     CDS             complement(8186..8710)
                     /locus_tag="VG272_05955"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HWF13259.1"
                     /translation="MRSSPRKFSSEDDLYIAALNSLARRAYSVYEMRTYLERRAEDKD
                     VVKVVLERLKHLNYLDDARYARQFVRLHTELRKQGAFRIARDLRARGVPDRHIEAALA
                     ERSPESNEAALVRERLKGRIKLLRGPLDERRVASLYRSLLRAGFAADTIRRELTALTK
                     QSVEEIPESSGGED"
     gene            complement(8817..9962)
                     /locus_tag="VG272_05960"
     CDS             complement(8817..9962)
                     /locus_tag="VG272_05960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HWF13260.1"
                     /translation="MALSIDDLLRRAVETKASDLHLKVGNHPCLRIDGSLTPLTDVPR
                     VTPEEMLSMAFSMMTNRQKQKFKETAELDMAYGVAGLGRFRVNVFQQRGNVGMVLRVI
                     PTKIRTIEELNLPHVVETICEEQRGPVLVTGTTGSGKSTTLAAMIDRINTTRSDHVIT
                     IEDPIEFLHRDKKSFINQREVEVDTANFSTALRAGLRQDPDVILVGEMRDLETIATAL
                     LAAETGHLVFSTLHTLDATETIQRIIAVFPPPEQKQIRLQMAATLKAVVSQRLVHTAN
                     ENGRVPAVEVMIATAYIRDCIINPDKTRLIHDAIAAGTSQYGMQTFDQSLFDLYSREL
                     VTYEEALLRASNPDDFKLRVQGIRSSSDSAREEMERTMTEFDQFGKR"
     gene            complement(10117..10455)
                     /locus_tag="VG272_05965"
     CDS             complement(10117..10455)
                     /locus_tag="VG272_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF13261.1"
                     /translation="MRMTLSQPPKKTRRQMLEEFVASKPTDAFARYGLAMECSNTGDA
                     AAADENFNALMTSHPDYVSAYFQYGQFLAKSGRTNEARAALTSGIAVAQRIGDDHARS
                     EMEAALAELG"
     gene            10556..11086
                     /locus_tag="VG272_05970"
     CDS             10556..11086
                     /locus_tag="VG272_05970"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HWF13262.1"
                     /translation="MIKKTSRNSTKEKKQSEKVLPGRLVSASRTEMTELVLPQDSNLR
                     GNILGGRVMHMIDIAGAIAAHRYCHRQVVTASVDHLDFLNPVRVGDLIVLEAQVNRAF
                     HTSVEVGVEVFSEDSAVGIRKHTTTAFLTFVALDEAGKPVPVPPVIAKSSEEKRRYKE
                     AGERRELRIKARKKNK"
     gene            complement(11229..12038)
                     /locus_tag="VG272_05975"
     CDS             complement(11229..12038)
                     /locus_tag="VG272_05975"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HWF13263.1"
                     /translation="MTCEQLALLYDEYALGVLDGEERAELEQHLARACPKCAPGVAEA
                     RGFIAQLAVTAPSVEPPSELRAKIMDAVNASGDAARATRRIEQKAVTPPRALFPAWAW
                     LAAAALALITGYTIRQMSRQTDQLAELRKQMKIATMQTQALQNQLEMDRMVASVMLSP
                     ESRQLKLESADKNMPVVHAYMHPHMGVAITADQMPSMPSARTLQLWFVPKSGKPVSAA
                     IFHPDSTGQIAFVAPVTISLNEIAALAVTDEPAGGSPQPTTPIAWIAKVSN"
     gene            complement(12038..12631)
                     /locus_tag="VG272_05980"
     CDS             complement(12038..12631)
                     /locus_tag="VG272_05980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF13264.1"
                     /translation="MLFAVVQPWPEGTQLTDAALMERILQGDSGALESLYDRYGRPVY
                     SLVLRIAQHPSSAEEIVQDVFLQLWRSAERFQIERGPLEPWLFTMARNRALDFLRLKR
                     EKQRRREDSDSDILPSAVVRSDPEGEIDRSRRAEKIRALMTSLPDAQRKAIELAFFEG
                     LSHSEIAASTGEALGTVKSWIRTGLLRLRQSLGEASS"
     gene            complement(12832..14250)
                     /gene="hemG"
                     /locus_tag="VG272_05985"
     CDS             complement(12832..14250)
                     /gene="hemG"
                     /locus_tag="VG272_05985"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HWF13265.1"
                     /translation="MSTSAQTAYPVLVIGGGISGLACAYHLQQAGIPVHAVEAGARAG
                     GVIATKGKDWFRFDIGPQSFLTTEPLLKLINALGLNDQLLHADPRAPRYILLHGKMVP
                     APMSPPSLFSTPLFSGGTKWRLLTEIMRRTQPPAEDESIADFVRRKFGPELLERLVTP
                     FVSGVYAGDPERLSMRASFPKIHEFESQYGSVLRGAIKSRPAKGTPRAELCSFRDGME
                     TLPRAIAARLVSSLHFETSVTAVRHGKANGKPWFEIDIARRDQHETLAASAVIFATPT
                     QVTSQILRNLSESFAPIFSQIEYAPVAVLSAGYRREQFQPPPDGFGFLVPRSEGLHAL
                     GTVFNSSLFPGRAPEGMVSLTSFSGGATDPKFCELSEREITETICGEVARVLGIKDQP
                     VTTNLQRYAHALPQYNLGHTQLVKSLETLAGSIPGLFLTGNYLSGPSIGSSVEQAFRT
                     ADAARIYLASIGEAGVGAVAHA"
     gene            complement(14273..15685)
                     /gene="hemN"
                     /locus_tag="VG272_05990"
     CDS             complement(14273..15685)
                     /gene="hemN"
                     /locus_tag="VG272_05990"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HWF13266.1"
                     /translation="MSDSNLHLRLNRERFEISEEFLEKYNRPGPRYTSYPTAPVWQDN
                     FGPDDLEQFYATANAARTPVSLYMHLPFCESLCLFCACNVVITKDHSVAPPYIETLER
                     EIEHVSRFVSRERPVVQFHWGGGTPTYLTPEQMEGLFRFTAERFTFAPDAEIGIEIDP
                     RVTTQAHLETLRRLGFNRLSMGIQDFHPEVQLAIHRVQPLEMTRSLIESARKLGFDSI
                     NVDLIYGLPLQTAERFAHTVEQVVAIAPDRVAMFSYAHVPWLRKQQGALATRLPEGME
                     KFHIFRAGLESFLNAGYQYIGMDHFARPNDELAVAQRERTLHRNFQGYTTKAGADLYG
                     MGVSAISGLSMCYAQNDRDVTHYRERVESRGIATMRGYRLTEDDLLRRTVINRVLCHG
                     VLINSEIEQEFGINFNETFAPEIERLAPFVSDGLIELENGVLRTTLLGRIFIRNIAMV
                     FDPYLEKQKLESRPLFSKTL"
     gene            complement(15741..16817)
                     /gene="hemE"
                     /locus_tag="VG272_05995"
     CDS             complement(15741..16817)
                     /gene="hemE"
                     /locus_tag="VG272_05995"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HWF13267.1"
                     /translation="MTEFAESVSQKTLFLRACRCEPVERVPVWIMRQAGRYLPEYRAI
                     RQKHNFLEVCKTPELAIEVSLQPHRILGVDAVIVFSDILIPVGAMGLPFELSDKGPVI
                     EPPLRTAAQIRELSSFDPERDTGFLMNAIRGLVRELGPTVPVLGFAAAPWTLACYMIE
                     GHHKDGFPAAKTLLYSEPELFRNLLERIAHATAVYLRAQIAAGASAVQLFDTWVGELN
                     VDQYREFALPATQLLIAEIDAGDTPVILYSKASNHLLTAAAESGANVLSVDWRVDLAT
                     VREQVGPGIALQGNVDPCMLLGPEAGVRKAARAAIESSRGVGHILNLGHGILQQTPVE
                     NACAFVQEGQSISVGDEVRAEDAI"
     gene            complement(16946..17863)
                     /locus_tag="VG272_06000"
     CDS             complement(16946..17863)
                     /locus_tag="VG272_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13268.1"
                     /translation="MEINTIPGVRPGWLLATLILQFLTFLSVVPGFGTLLSWSLHPYY
                     GTSLLPLAIHLSIYAGLPGVSLFGIWRIQRWGWILAVIADGGMCAQALFQNGVGLVRG
                     PDFLEVNVWKFAALAVLLQKPVRTQFFGPKSHLGKTIPWMVATRWLERTLWLDKVPWR
                     RILADVHNLADRVMCVVAYFLAAVTATIASAAFTVAFLGGQRPSGGAGILFVIIMYSP
                     FLSIPAFLFSLVLTIVVRILGPKRRWVWLVVGALVGPGLSFAYLGLKTPSQILALTPF
                     MGIFPAWTCYVLYPWAFSRPHKIGKTILR"
     gene            complement(18161..18814)
                     /locus_tag="VG272_06005"
     CDS             complement(18161..18814)
                     /locus_tag="VG272_06005"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HWF13269.1"
                     /translation="MSLKTKLLRLAAFVVVSALIFSAAPLSADILDDWSAVKPPPLPQ
                     LKSVTLDGATTALLILDMMKMNCGARPRCVATVPNVKKLHDAARAAGALVWYSYVGSD
                     GQATPADQIDPGVMARDGEWVRQNGPDKFIGSNLDEKLKARGIKTVIVCGTSFQGVGI
                     GTGGGSAQRGYKVIIPIDCLSSDDPYMEQYSAYHLYKGAPAGVTSQVTLTRSTMLKF"
     gene            19064..19876
                     /gene="ccsA"
                     /locus_tag="VG272_06010"
     CDS             19064..19876
                     /gene="ccsA"
                     /locus_tag="VG272_06010"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HWF13270.1"
                     /translation="MTSPYVIATLFLYAASLALYIWNLREPSASVGIGATTCLIGGLG
                     LHYLALLARSRLIHAVPYDDLFGSLSLFAWLLAATYLCLEAIHRRRVVGPFVLPIVLA
                     VFLLAHVPEAKLVKAPPAQGPLFAFHVTLNILAYSAFAISFVLSIIFLIQNRRLRDHK
                     LGDVGWRFPALDVLDRMTRSSVLIGCISLAAGMTAGMIWAHRLEGRYWDGDPKVIVTV
                     VILLAYVGYTFLSRTSAWRGARASALCIFNFMFVIFSYSIVNRYLSHFHRYF"
     gene            19925..21202
                     /gene="hemA"
                     /locus_tag="VG272_06015"
     CDS             19925..21202
                     /gene="hemA"
                     /locus_tag="VG272_06015"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HWF13271.1"
                     /translation="MEIVLIGLNHKTASVDLRERVAFTPDQAREAAEQLRSRGILEES
                     LVLSTCNRSELYGVPSESTANSAGALELFIASFHQLSPSDLDHSLYRHRDSQAVRHLF
                     RVSAGLDSMLLGEAEILGQVRDAYQIALDRGETGPVLNRMFQAALEVGKRVRNETELG
                     TRPVSVAFASVKLAERIFGKLNTHRALIVGAGTTSEQVVKHLCDRGIKQLRVINRTPE
                     HAVDLAARFDGEVVPWENLSAALEWPDLIVTSVSSTEPILSRAMINKAMAARGNRALL
                     MIDLGVPRNVAPDVADIYNTYLYNIDDLTEIVEQNKKVRVAEIPRAEAIIDEQVDKFL
                     HWQAGVAAGAVLGDLKSKLAAEREAFMKERFAAMPNLSDEDRAQVAAMMQEFTERMLI
                     TPAEKMRGIPELRRKLQNFEAIRDLFHLDREKP"
     gene            21199..22128
                     /gene="hemC"
                     /locus_tag="VG272_06020"
     CDS             21199..22128
                     /gene="hemC"
                     /locus_tag="VG272_06020"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HWF13272.1"
                     /translation="MKALRIGTRGSALALWQSRSIAHALADRTGVEPDLIIIKTSGDK
                     FQKLSFSQIGTKGVFIKELEDALIEERIDIAVHSMKDVPTELPEGLTIAAIGKREDVR
                     DALLSLNGGTLASLPQGARVGTSSLRRQSQLLAARPDLRMLELRGNVDTRIEKLKRGS
                     YDAIVLAKAGLDRLGLSANISEVLSYEISLPAAGQGAIGIEARSADAGTLRALAGLED
                     AETRAAVTAERAALAGLGGGCQVPIGAWGRMENGKLLLDVAVLSPDGTQRLVEKDAGA
                     RGEAEAIGRRVTQKLRERGATALLERENRGTPA"
     gene            22125..22934
                     /locus_tag="VG272_06025"
     CDS             22125..22934
                     /locus_tag="VG272_06025"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HWF13273.1"
                     /translation="MNVSSPLDGKKIVLTRAVGQTRELKARLEKMGAVVLLFPAVNFS
                     EPTDTKELDQAINSLNEFDWILFTSANAVRFFAGRCRKLEVEPSHDVRFRCAAVGPAT
                     ASAVAAEGFPVDHVAQEFIGSALARELSSLLQGGKVLLPRSERARPDLPAALRSVGAL
                     VTEIVAYHTGGVGTIDPVVMSAIREADVDVISFFSPSAIENMRVELGEELLSRLGAKA
                     KMAAVGPTTAAALRGAGLPVAIQAPLATAESMAVAIATYFSTNTGSKARAI"
     gene            22931..23908
                     /gene="hemB"
                     /locus_tag="VG272_06030"
     CDS             22931..23908
                     /gene="hemB"
                     /locus_tag="VG272_06030"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HWF13274.1"
                     /translation="MNFPVTRPRRLRRNEAIRSLARENRLSTQGFIYPMFVCPGKNVR
                     NPISSMPGVAQQSVDMLVEECREVEQLGIPGIILFGIPDKKDALGSEAYAADGIVQRA
                     IEGIRKANLNLLVMTDVCLCEYTDHGHCGIIENGEVKNDPTLELLAKESVSHARCGAD
                     IVAPSDMMDGRVGAIRKALDNAGFQDVAIMSYAAKYCSGFYGPFREAAESTPQFGDRR
                     GYQMDPANAREALREVELDLAEGADMVMVKPALAYLDIIQRVRETFSVPVGAYNVSGE
                     FAMVKAAAANGWIDEKRIAMEILTGIHRAGASIILTYHAKDAARWLKNS"
     gene            23936..25249
                     /gene="hemL"
                     /locus_tag="VG272_06035"
     CDS             23936..25249
                     /gene="hemL"
                     /locus_tag="VG272_06035"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HWF13275.1"
                     /translation="MSAPNPTKLRRPHSLEIFHRATDVLVGGVNSPVRAFRAVGGEPI
                     VVDRAAGARLWDADGNEYIDYVCSWGALILGHTHPKVVQAISDQARRGTSYGMPTELE
                     VELATRIRKALPSCEKVRFVSSGTEATMSAVRLARAATNRDLIVKFDGCYHGHSDSFL
                     SEAGSGLATLGIAACPGVPQALAELTLNAQYNDAAAVEKLFDLHPGKIGAVIVEPVAA
                     NMGVVAPKPEFLKALREITSRHGAVLIFDEVITGFRLCYGGAQIVFGITPDLTTLGKI
                     IGGGLPVAAYGGRRDLMDRVAPLGPVYQAGTLSGNPLAMSAGIASLDLLATPGFYEAL
                     DARAKRLGDGMVAALRETGTPATAVRVGSLLTVFFSREEITDYAAAKKCDTRRFADFF
                     RGMLDRGIFIAPSQYEALFVSAAHTDADIDRTIAAFRESLSAVAE"
     gene            25337..25879
                     /locus_tag="VG272_06040"
     CDS             25337..25879
                     /locus_tag="VG272_06040"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HWF13276.1"
                     /translation="MADELSELRENAEHGRENPSFVHVSVTMAILAVCVAIVSLMGHR
                     SHTEELMMQNRATDQWAYYQAKNIRLHNYQMSIDLLSMIEFKNKDQAAKVHDKYQSEV
                     ERYTKEQTEIEEKAKEDENESASAQHKADRYDLGEVFLEIALVISSLALLSGKRIFWV
                     LGLMSGLTGLGIAASVFLLH"
     gene            complement(25963..26121)
                     /locus_tag="VG272_06045"
     CDS             complement(25963..26121)
                     /locus_tag="VG272_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13277.1"
                     /translation="MDRATLDPGGTICFTGKKPAPEEERHRQLMQRLDRLTEEIATLR
                     LRPAPPSS"
     gene            complement(26140..26553)
                     /locus_tag="VG272_06050"
     CDS             complement(26140..26553)
                     /locus_tag="VG272_06050"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HWF13278.1"
                     /translation="MNSTLMNHMFVLAIPVAEKILRPIVVYVFLVLGLRLAGKRELAQ
                     LNPFDLVVLLTLSNTVQNAIIGDDNSVTGGIIGAATLLLLNYLVVRFLFRHEKIDRFV
                     EGDTSILIENGVILEDRLTKELLSVQDLQTAAHKQ"
     gene            26988..27374
                     /gene="hisI"
                     /locus_tag="VG272_06055"
     CDS             26988..27374
                     /gene="hisI"
                     /locus_tag="VG272_06055"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF000768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HWF13279.1"
                     /translation="MDLDFKKLDGLIPAVIQDATSGEVLMLGFMNSEAYAATQAIGEV
                     TFFSRSRNKMWRKGEQSGHVLRVREILVDCDEDTLLIRVDAVGPGVCHEGYRTCFFRQ
                     ITPSGEEKIVGQRTFDPNAVYGKDAK"
     gene            27374..28246
                     /gene="hisG"
                     /locus_tag="VG272_06060"
     CDS             27374..28246
                     /gene="hisG"
                     /locus_tag="VG272_06060"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HWF13280.1"
                     /translation="MGVLRLGIPKGSLQDATVQLFGKAGWRISINSRSYFPGIDDLEI
                     NCMLVRAQEMARYVETGALDAGITGRDWVFETGSKVTEIGEMMYSKQSLAPVRWVLAV
                     PEDGPIQSVKDLAGKVIATEVVNLTKQYLARNDVEARVEFSWGATEVKVPQLADAIVE
                     VTETGSSLRANHLRIVDTILESRTVFIANHSAAADPWKAEKISNIFLLLSGAIAAYTK
                     VGIMLNARKEQLAAVLAVLPALKNPTVSTLSDPGWVAVNTIIDEDVVRSIIPRLKAAG
                     AQGIVEYPLNKIVV"
     gene            28264..29517
                     /gene="hisD"
                     /locus_tag="VG272_06065"
     CDS             28264..29517
                     /gene="hisD"
                     /locus_tag="VG272_06065"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HWF13281.1"
                     /translation="MRILKLTDRVAEKFFAQRSSYDAAADKVAAEILADVRKNGDAAL
                     FRWALHLDKLSLTKKSLWMSRAEIRVASRGVSRELVRAIEHAAKNIRRVAEAQRPRSW
                     SITVEPGVRVGQRVTPLGTIGCYIPGGRFSLFSTLLMTAIPAQVAGVKRIVVACPKPS
                     PALFAAAEILGITEIARMGGAQAIGAFAYGTQSIPKVDKICGPGNRYVTAAKRIVSND
                     CAIDMPAGPTEVLILATGGSPLFIAADFLAQAEHDPDAIALLITPSARLAKSVAAEVD
                     KQLKDLPETNPAYKSLQRACAILVAPSLDAAIRFTNRVAPEHLSIPQANKSLIEKLSE
                     AGSIFLGPWSAQPIGDYASGTNHVLPTSGWAHTRGGLSVTDFVKCSSTQEISSAGLKR
                     LLPVVTAMARAEGLEAHARAVEVRE"
     gene            29514..30590
                     /gene="hisC"
                     /locus_tag="VG272_06070"
     CDS             29514..30590
                     /gene="hisC"
                     /locus_tag="VG272_06070"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HWF13282.1"
                     /translation="MKRTKPQSIAIRKAVMGLAPYNAPSEGRAKKLRLDFNENTVGCS
                     PVVIRALSRMTAKEMAVYPEYQETTKRLARFFGVRPAEMHLTNGIDDALHLIADTFIN
                     EGDSVLVVEPTFDMYRFFAELAGASVVALRYDDEMRFPVDAVIRVLQQPAKRCPRVLY
                     IANPNNPTGTLVQREELRRILQAAAHTLVLMDEAYFDFSGLTILSWIRRYSNLLVART
                     FSKSAGLAALRIGCLFGKPELLAAMRRACTPYPVNSAALVAAEAAIRDPRFLRNYTRE
                     VLASRAMLEKGLVRLGARIYPSSANFVLADFGPGARRLVRVLEKRGILVRGRKDFPRE
                     GFIRISAGTRADTRKVLRAMKGIL"
     gene            30587..31273
                     /locus_tag="VG272_06075"
     CDS             30587..31273
                     /locus_tag="VG272_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HWF13283.1"
                     /translation="MKLSPKLIIFDVDGVLLDVHGSFHKSIIDTVHFFTKKHVTTAGI
                     HDWKNRSGYNDDWRLTTDWVASLGPAVPYEEVKERFMKFYWGTKSKPGNVAKERWLIT
                     HKRLERWSRRAELAIFTGRTHRELSHTLVGRDAHGIFRRVVTMDDVKKGKPDPEGLRL
                     LLDGMPVNEALYLGDNIDDALSAQHAGVPFLGVLPHGSEAHNARAKRLRELGALKILH
                     SVRDLEKLWK"
     gene            31270..31857
                     /gene="hisB"
                     /locus_tag="VG272_06080"
     CDS             31270..31857
                     /gene="hisB"
                     /locus_tag="VG272_06080"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HWF13284.1"
                     /translation="MRKAKLRRTTKETDIRVSLNLDGQGRAKIATGIPFLDHMLTLVA
                     RHGAMDLELAARGDLEVDQHHTVEDTGIVLGEAVRKALGSKKGILRAGYFLMPMDETL
                     AAAAIDFSGRPHLVCKWKFSAKHVGGMQTELLEDFFGGFAQAAGANVHLSLLYGRSSH
                     HQVEAIFKAFARALRFAATRDKQLKKVLPSTKGLL"
     gene            31854..32468
                     /gene="hisH"
                     /locus_tag="VG272_06085"
     CDS             31854..32468
                     /gene="hisH"
                     /locus_tag="VG272_06085"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HWF13285.1"
                     /translation="MNIALVEYGAGNLPSVERALTRLGVESRRASTPEALADCDALIL
                     PGVGHFGALMRSLGDRGLVAPLREAVSRGTPLLGICLGLQAMFATSEEAPGDTGLALF
                     PQKVSALPPADRLPHMGWNQLRRARSSKLLEGVPGDAYFYFAHSYAALDAGEAGVAYC
                     DHCASFVAVLEKGNVFATQFHPEKSGPVGAKILSNFVQHAGGKR"
     gene            32465..33229
                     /gene="hisF"
                     /locus_tag="VG272_06090"
     CDS             32465..33229
                     /gene="hisF"
                     /locus_tag="VG272_06090"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HWF13286.1"
                     /translation="MSLARRIIPCLDTDGERVVKGVNFVNLRDAGDPAALAARYNIEG
                     ADELVVLDIAASRDHRPTFLETIRRVAAELAIPLTAGGGVANVDEGRAILRAGADKLT
                     VNTAAIRNPVLLTELAAEFGSQAVVLAIDAKRVGDRWEAFVRGGSVPTGIDAVAWARR
                     GVDCGAGEILITSMDKDGTRSGFDCPLTAAISRAVTVPVIASGGADTPETFLEVFGEG
                     RADAALAASIFHDSIQRLGELKHFLGERGISVRQTG"
     gene            33238..33924
                     /locus_tag="VG272_06095"
     CDS             33238..33924
                     /locus_tag="VG272_06095"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HWF13287.1"
                     /translation="MMIPCIDLQDGRAVQLVHGRKLKLAVDDVLGLLDRFGNHPLIHI
                     IDLDAAMRKGSNGRLVRGLCIAAKKRKMRVRVGGGIHTVAQAAKIAAWGAEKIIVGSA
                     AFKDGNVNDKFLSRLAGKIGKQRVVVALDTEGGKILVRGWQDKLRLRPDEVIPQLEKY
                     ASGFLCTYVDAEGTMRGTNLKWFRSLRKATARPITAAGGIRSRKEIQALERMKMDAAV
                     GMALYTGKLS"
     gene            complement(33980..34396)
                     /locus_tag="VG272_06100"
     CDS             complement(33980..34396)
                     /locus_tag="VG272_06100"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWF13288.1"
                     /translation="MSRIFEALRNAQFNRSDKSKSNSIEVKLGEISDRRRSKRWPMDI
                     SVYVYGHGSGKETFHEEAHTLNVNANGALLLLSVPVQKGQLLLLTNQHTEEEMDCRVV
                     YLGTRHSRTVETGVVFPRPNLEFWQIHTPSSDDSAA"
     gene            34695..35618
                     /locus_tag="VG272_06105"
     CDS             34695..35618
                     /locus_tag="VG272_06105"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HWF13289.1"
                     /translation="MPRSAKIAKESASTVRIGPAGWSYADWAGIVYPARKPFGFHEAA
                     YLSHFFDTIEINTSFYHPVRPEHCKNWIQLVSGNPRFLFTAKLWQKFTHEADASREDE
                     SVVRAGFDVLRDARKLGAVLLQFPFSFHKTEENTAYLSKLLKKFSDYPLVVEVRHATW
                     NDKAFYAMLHERGVGFCNIDQPVIGHSMKPTERTTARVGYIRLHGRRYDSWFSDDPAM
                     PPFERYNYLYSEEELEPWAARIKHVSESGESTFVITNNHFEGKGIVNALQLIHLLNQA
                     KVKIPEQLRQRYPELDRIATEPPVEPLLFPK"
     gene            35766..36086
                     /locus_tag="VG272_06110"
     CDS             35766..36086
                     /locus_tag="VG272_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13290.1"
                     /translation="MSGPLGGFGLLGPLGGVGGVGLFGPFGAGGLSGFAGGVGLLGPL
                     GGVGLFGPFGLPGFGSFGAGPFRSLGFLGLAGPLPGGFGPLGLFGGLFGLFGSLGVGF
                     PGCC"
     gene            36811..38115
                     /locus_tag="VG272_06115"
     CDS             36811..38115
                     /locus_tag="VG272_06115"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWF13291.1"
                     /translation="MGVMERLQTVRRMERPWWLAVIACTGAVVIGHFLRQLNIVFNPL
                     AWSSAEIISALLSFTIAASVLVRYYGTGNRVSLLLGLTFGVTGIIHLGAIFEVYDHFL
                     KHSEQVHVPVSWMIGQTLLGLLLLVACVINKWLPWPRDPGKNFIALSSIVAGVTCLVT
                     AIFLIFLSTPPIHPHSPTPRVWELFPAVIFLIAAVALHSVKESNSFAFDTALVWVAGL
                     NVISHLIASQSARLLDASAGVAELVNAVSYIVLLGATLLDNAHLFRQVRTLAISDSLT
                     GLANYRHLVDVLQGELERSGRTNRPFSVLLMDLDGLKKINDVHGHVIGSRSLCRVASI
                     LRLNCRSIDTAARYGGDEFALILPETTMMAAEQVADRVRTCLMADQEVPRLTISIGVA
                     TFPKCGLTVQELLEFADRALYARKEISKRGKVERRRNVPDSN"
     gene            complement(38200..38550)
                     /locus_tag="VG272_06120"
     CDS             complement(38200..38550)
                     /locus_tag="VG272_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13292.1"
                     /translation="MNSSDYLGTMDLPERRISPRIDLDIDLTVYCRLPDEDAFYGQAK
                     AISGNANGGVFLFSIPVVEGQDLLLINNVTSAEQICNVLKVNIRDFQTSEVVVGFSLP
                     NSDFWKKSATYQSL"
BASE COUNT         8512 a        10167 c        11256 g         8964 t
ORIGIN      
        1 ccgctgcagt tgatttttgc ggggccttca ttggggtatt tggtattttc cgcgcggacg
       61 attccgtgtc tgttgcttcg cttgtagata cactcgctga tgcggacggc gcagatgtgg
      121 ccgaattaaa ttttggagga ctctgcatga ccggagcatc cggcggcgga acgaatggcg
      181 atttcgcagt cttttccgcg agtcttacag gcggcttggc ggatgtgttt gcagcaaaca
      241 tcttatccgt ttttgaggtc gttgcgtcca tcgctgtggg cccggcggcg ggagcggttt
      301 cgcgcgcagc cgcttcggct gcggcagaag tgccgtacac atccacgacc aggcggtatg
      361 gatcgggcag caggaagacg gagaagtccg cgacgtgatt aatttccagc acgacgcgcg
      421 ctacgctggc ctgatactgc gcgacgcgca cggttttcag gaacgggctt tccacatcga
      481 tcggcttgcg catgagggcc gtattgattt tcgcgccctc aatgtcgaaa taaatgcggt
      541 ccgggccgga aatgcgcgcg gcttgatact tcactttcga tcccacgtcg atcacgattc
      601 gagtataggt gtccgcgttc catgtgcgaa tacgcgatac ttgcgggctt gaggaattct
      661 ggttcgagct ttcttccggc gatccgggtt tcgtgtccgt cgtcggcgga actttttcag
      721 cggaaatatt cttgggactc atccggtcag agcgggtatt tgaaacggga atcggctgcg
      781 ccgctttcgc cgcggctgca cttgcagcgt tcaacttgtc gatgctcgcg cgtacttccg
      841 cggcgtgcgt cgaatgcgga tgcgccgaca aaaaatcctc gtacgatttc tgcgctgccc
      901 cggcatcgtg caaatcatcc tgctgaatct gcgccacggc cagctgcgca tcctcgcggt
      961 atttgctggt tggatattct ttcaggagga acgcaaagga cttgatcgag cgctgataat
     1021 atttcgcgtc aaacaaatcg cccatcgtgc ggaaaagttc cgcaacttga ttcagcgagg
     1081 cgggaacatt ctccgcgtgc gaagtcgtta gatacacgcg ctggtactgg atgacaagcg
     1141 ccgtgtagtc cttcagcgtg cgcgcgcttt ccggacgcgc ttccagcacc tggcgcgcct
     1201 tttcagcgcg gtcgaattga gcattggcgg cgtcgcggcg ggatgacgca ttatcgtcag
     1261 ctcgcgcaat cgtttgtgaa atcaggggaa aaaggcacaa gacaagggcg gacgcgaaaa
     1321 aaacgcgcag gattggccgg attttagcgg ctttatgccg cactgtctgc ctcactcgtt
     1381 cgtaaaaacg acccgtccat ctgactgcac cgtgcggtaa ataggccgct gctgctcgaa
     1441 cgcacgggga agccgatcag atccgggacg gtagtaggaa tcggtaactt tctgaacgta
     1501 atttcgcgtc tcccggtacc gggggatgcc cccagcacga tccacggcgc ccggccctgc
     1561 gttgtatgca gccaaggcct tatcgagatc gctattatac cgctccagga gcgaatgtag
     1621 atagcgaact ccaccgtcaa ggttctcctt cgggtctagc gcgttgctta cgcccagctg
     1681 ctgcgctgtt cccggaacca gttgcataag cccaagcgcc ccgactcgag aaacggcatt
     1741 cgtgttccag ttcgattccg tctgaatcac ggcccgaacc agcgccggat caacatgata
     1801 acgcgctgaa acttctcgaa tcatttcggt gatcttctcc cgattagcct ggtcaaacgc
     1861 aggcacctca gaagagactc cgaaagaggg cggggctgtg cgcagggtaa acccgttggc
     1921 agccgtcttc aaaaggggcg ctttggatgt gtgagcaacg gatgccggtt ccgcattgat
     1981 aaagacccgt ttgccgctca cgtccgtata ggaaacgatc tggcctcggg cgggaatagc
     2041 ccataagcca agcgctccca agaaaatagt caccgatttg tttagccgac gcatacgaag
     2101 ttctgggaag gcagtgcgat tccgttccta ttgagacaga ccaatcaccg cgacagggca
     2161 acagggagag agtaattcgc cctggacgtt cctgactctg cgaagggaat tataacaact
     2221 ggtcgattag ctggtacaca tggtcgaggg cactttttag ggcagatgtg tcttttccgc
     2281 cagcctcggc cagatcaggg cggcccccgc ccgatccgcc gacttccttg gccgtggcct
     2341 gtatgatctt tcctgcgtgt aacttaggga tgagatcctt ggtaatacca gcgagcagcg
     2401 cgaccttgcc gtcttcaatc gtgccgagga cgacgacccc tgagccgagc ttctggcgga
     2461 ggctatctac gacctggcgc atggtttccc ggtctacacc ttcgagttgg gcggagatga
     2521 cttttacgcc ctttatttcg tgcgcccggg ccaccaaatc gtccagtttc gaaagggcgc
     2581 ctttgcgttt cagggtttcc agctgctttt cgagttgctt ttgggtctgc tccatgcgct
     2641 cgatgccggc gagcaagttt tcgtcgggcg tggccatgcg ggcggacaag tcgcgtaagg
     2701 tttgctgagc gcgctggtaa tcggcaagag cggctgcgcc agtgatggct tcgatgcggc
     2761 ggactcctgc ggcggcggat tgctcgacca cgattttgaa tacgccgatg tcaccggtgc
     2821 gggtgacgtg cgtgccgccg caaagctcct tgctgtagaa gcctcgcggc gcattagcat
     2881 cgggaatggt cacgacgcga acgttttgct cggggtagcg gtcaccgaaa aatgcgagag
     2941 cgccggaagc cagagcgtcg tcaatcgtgg tgacgttcgt ttggattacg ttattgagca
     3001 gaatttcttc gttcacttgc tgctcgatgt cgcgcagatc cgcgggatcg attgcggcga
     3061 agtgcgagaa atcgaagcgc aagcgtgctg gctcgacgag cgatcccgct tgttttacgt
     3121 gcgtgcccag aatattgcgc agcgctgcgt gcatcaagtg cgtgccggtg tggttgcgct
     3181 tgatgtgttc gcggcgcgcg gcgtccgctt cgccggcgat gaggtcgcct acggcaagct
     3241 tttcttttgc gatcacgcga tgggcgatca ggccggagac gggataataa gcgccacgta
     3301 cttcgcccag ttgcatcgtg tgctcgttat tccaaagcgt gccgatgtca gaaacttgtc
     3361 cgccggattc ggagtagaac acggtgcggt cgagaacgat ttctccttca gcgccggcgg
     3421 gtaattcgtt gatcaggcca tctttcgtga cgatggcttc gatgcggcga ttttttgcgg
     3481 tcgtgctgaa ataaaaatcc ggctcggttt taaatctttc ggcgagtttt gcgtagacag
     3541 gattggcgac gtctttgtgg ccgcctttcc acgaagctcg tgcgcgcgtg cgctgtgctt
     3601 ccattgcctg ctggaatcca gttcggtcga actcaactcc gatatcgcgc acggcgtcct
     3661 gtatgaaatc caacggaagg ccaaaggtgt catagagttt gaacgcacgc tcacctttgt
     3721 ataccacctt ctttccactt agctttattg cggccatcct gggaccgagg gtgaggtaat
     3781 cgtattttcg cgatgcttcg tttctggtta ggttatttcc aggtccaggc agttccacat
     3841 ctagtaaggc caattccagg tcttcttcca gccgacctaa cccttcgctc aaggtatgcg
     3901 cgaatcgtgt ttcctcgcta aggatgattt tacagacctt ctcgatggaa tcattcaggt
     3961 ccgggtaggc atctcgcatc aaggctgtga ccgtgcgtga catttcagcc atgaaaggtt
     4021 ctgcggagcc tagcaggcgt ccatggcgaa tcccgcggcg catgattttt cggagcacgt
     4081 agcctcggcc ctcgtttgat gggatgacgc cgtcggaaat aaggaatgtt gtagcccgag
     4141 cgtggtcggc aatgatgcga agtgaagcgt cgcttcgctg ggaagctcct aggcgtgtgc
     4201 cggtgagttc tcctgcgcgt tcgataagcg gtacaaacag atccgtatcg aagttgctga
     4261 tttttccttg caagacagct gcgaggcgtt cgaggcccgc tcccgtatcg atggaatgct
     4321 taggtagatc gcttactttc gcccagtcag gcccggcttg acggttgtac tccatgaaaa
     4381 cgagattcca gagttcgaca tagcgtccgc agtcgcaggg aaacttgcaa tccatgtggc
     4441 cctgatcaga agcttttgcc cccatatcgt aatgtatttc gctacacgga ccgcaggggc
     4501 cggtgtctcc catcgcccaa aaattctctt tcaaaccggg gatggcatag atgcgctcaa
     4561 gtgctacttt ctgctcaagc cagaaattac gtgcttctga gtcttcaatg agttccatgc
     4621 cagggaatat ctcggcacca ccaaaaactg tgatgaatag cttctctttg gggatattga
     4681 accagttagg ggatgtcacc aactcccatg cataagcgat tgcctcgcgc ttggaatatg
     4741 gaaactgatc tccaactact ccaaccggtc caaacgaaaa atttccgagc atttcaaaaa
     4801 aagtatggtg gcggttggtg aagcccacgt tttcgaggtc gttgtgcttg ccgccggcgc
     4861 gaacgcattt ttgggaggtg gtggcgcgcg tgtaatcgcg ctgctcgagg cccaggaaaa
     4921 cgtccttgaa ttggttcatt ccggcgttcg taaaaagcag cgtaggatca ttcgtcggga
     4981 cgagcgacga gctgcgtacg atgcgatggc cgcgctcggc gaagaattcc aggaactttg
     5041 tgcgaatgtc gttaccgctt ggcaaaggaa ccccagtatt tcgtgatttg tgactcgtga
     5101 ctcgtaattc gtgactagtg attggtgact agtgactggt gactcgtaga tgtaggagct
     5161 ggaatatgcg ctgcccacga tccatttttt ggcgcagtcc tgtcagaaaa acatatccaa
     5221 tccagcaaaa cattatggca gacaggggac acgctcgcaa cgcgggcccc cggtattgat
     5281 gatcactaaa cttaagaatg ttggtcctgc aaaaacaacg gaaagggctc taacgcagaa
     5341 aacacagaga aattcggaga gacacagaga gaaacgatat aaaggacaat ttcgtggatg
     5401 gatgagccga agagacagag acaaattgaa ttacttcaaa tgccacacta cggacacaag
     5461 atcggtttgc tgttttcatt gtctttcttt tctcaacgtt ttcctctgtg tcctctgtgt
     5521 taaagatttt aacgatttca aagttgagga ggccgcgccg cctcataaaa agcgctccaa
     5581 tttgactgcg gatcatagtt gtcacaatcc accgtaaggg aaatcaatcc gcgaatatgt
     5641 gcaacttgcg gatgcgatca atccatttat tgagtttgac ggttttacaa cactccgtta
     5701 tcttagcttt tcatgggctt aagggccttt tacgggaaat gcggctaggg aagttttatg
     5761 gatttaaaat gcgccaatcc gatttgcaat tctgaatatg aaaatgggcg tggtcgttta
     5821 ctacgcattc agcaaactct ttccccggaa aaaccgcctt cccattggca cggcatgaag
     5881 cactactggc tgtgcgcgcg gtgcagcgac aaattcacga ttgaaattca aaagggcacg
     5941 ggcgtgttgc tcgcggagaa gttggaggaa ggcgcggaga ctcaagctgc ctacaccttg
     6001 ctggaatctg aaacgcttcc caaacaaggc gtcaaaccgt tgctgcctcg cttaaccagg
     6061 tccagggccc gccacagaaa acagaaagtt gatttgacgc ccactaccgc gagcgcggcg
     6121 gacgtactcg aaatccgaaa aacggaaagg aggggataga ccccatgagt gaacagattg
     6181 gaaccatggc gggtgccatc tggcacgcgc tggaagcaaa tggtgaaatg acattgagca
     6241 agctgaaaaa ggaattaaac gccggatcgc cgctgtttga ctgggcgata ggctggctcg
     6301 cgcgtgaaga caaaatcatg ctgaccacgg aaaaacgaac cacgcgcgtc tgcctgaaag
     6361 gatggcacac gcaaagcatg cacgctgcgt agcctcaatc gccgaaaaat tctccatttt
     6421 gaacttccgc ccagtgaagc gccttcgtat gcgcaaattt ttaaatagcc cctgcaattt
     6481 tatatctcac cacaaagtgt gcatgccggg tttcgcctat ttgacttcac gaatgtttgg
     6541 atccgtgcac attgctttcg aggcatccca ataataagcg gctgcatctc tacgcgcata
     6601 tgtcgtaacc cgtgtcattg gtcttatttg actcatcgcc accgcatgaa gtggaggata
     6661 ttgatatgaa ttcaagagcc gcgaaatgtg gtgtcgcgct ctgcattttc agtttgctct
     6721 tcctgaattc acttcccatc agggcgcagg aagcgaatgc atcgttgtcc ggagcaatca
     6781 ccgccgcaac cggagcttcg gttgccaacg ccaaagttgc agtgaaaaat ttgtcgaccg
     6841 gcgaatcgag agaaacgcag accgattcga acggccatta caccattccg aacttgccac
     6901 cgggggatta cgaaatttcc gtgacggcgc cggaatacag cacgaatacg ttgaaggtta
     6961 cgctatccgc agcaggaccg aacaatgcaa acgtgacgct gacaggcgcg ctttcgcttt
     7021 cggatctcgg cttctcaccg gatcaggcac agggcaccgc ggcggatcag gcgcggctca
     7081 acaaacgctc gcacatgctg atgctgcacc agcgcatcgg cctgattgcc gctattccaa
     7141 tgattgcgtc tgtagcgact tcgttcggag ctggtggccg cggtaccagc acgaccgatc
     7201 gctatttgca tttggctctc ggctccgcga ccggagattt atatttcacc agcgcttact
     7261 tcgcgattcg tgctccctcc attccgggga cgcaacggcg cggacaaatt cgcgtgcaca
     7321 gggcgctcgc gtggattcat gggccgggaa tgattctcac gccgattctt ggagccatgg
     7381 cctttgacca aaaaagcaaa ggggaaaagg ttcatggcat tgccagcgcg catggggcgg
     7441 tggccatcgt aacggcggcg gcctatggcg ccgcgatcgc atccgtttca tttaaattct
     7501 aaaaaaagaa attgagttgg gccaaggatg aattgtgatt aagaaaatta tttgtgtgtt
     7561 tttatttctg gcgctgcctg gcctcgccgc tgcggacagt caatgggttc tggtggaatc
     7621 cacgctcacc taccacgtgt ctcatccgct gcaccagacg gagggagtga gtcacgcggc
     7681 gaagggaaaa ggcgtttgcc gcggtggaca atgcgatttc ctgattgcag ttccggtgaa
     7741 gtcgttcgat tccggcgata gcaatcgcga tctgcatatg ctgcaagtgg cgcgcggcgg
     7801 agaatttccg atgatcactg tgcgcacgca tttgcctgag gatgcgtcta ccctggcgac
     7861 aattcctgtg gacctggaag ttcagttcgc tgggcaaacc gtgccgtaca agcagattgc
     7921 atttcagcat gtagtcaagg gaaacgatac gttcattgcg gggacgattc cgctgacact
     7981 gacggatttt aaaattgatc cgccatcact cttggcgatt cctgtgaaaa atgaaattcc
     8041 ggttcgagtc gagatgaagt ggcggcagat gtagtttcgg tttaaatagc gagggcttca
     8101 gcctgcggat tttagtcctt gcatggtcga aaccccgctg gctgaagccc gagcacttga
     8161 aaacctgacc cattacacaa aactcctaat cttccccgcc tgaactttct ggaatttctt
     8221 ctaccgattg cttggtcagc gcggttaatt ctcggcgaat tgtgtcggct gcgaagccgg
     8281 cacggagcag gctgcgatag agcgatgcga cgcggcgctc atccagtgga ccacgcagca
     8341 atttgatgcg gcccttcagg cgctcgcgca cgagagcagc ttcattcgat tccggggagc
     8401 gctcggcgag ggctgcttcg atgtggcggt ccgggacgcc gcgcgcgcgc agatcccgcg
     8461 cgatgcggaa agcgccctgc tttctcaatt cggtgtgcag gcgcacgaac tggcgggcgt
     8521 agcgcgcatc atccagataa ttcaaatgtt ttaaccgctc cagcacgacc tttaccacgt
     8581 ccttatcttc cgcgcggcgt tcgaggtagg tcctcatttc gtaaaccgag taggcgcggc
     8641 gggcaaggct attgagcgcg gcgatgtaga gatcgtcctc ggaagaaaat tttcgcggtg
     8701 aagaacgcat gcgtgaagga tagagggaag ggtgcaggga agcaaggaga cggggacgga
     8761 ggcaggaggc aaggaagcag gggcaacgca actatgcatt cccaaacgaa gagcagctag
     8821 cgttttccga attggtcgaa ttcggtcatg gtgcgttcca tctcttcgcg agcggaatcg
     8881 gatgaggaac ggataccctg aacgcgcagc ttgaaatcgt ccgggttgga cgcacgcaag
     8941 agagcttctt cgtaggtgac cagttcgcgc gaatagagat cgaagagcga ctgatcgaat
     9001 gtctgcatgc cgtactggct ggtgccagcg gcgatggcgt catgaatcag gcgagtcttg
     9061 tccggattaa tgatgcaatc gcggatgtac gcggtggcga tcatgacttc gacggcggga
     9121 acgcggccat tttcgttggc agtatgcacg aggcgttggc tgaccacggc cttcagcgtc
     9181 gccgccatct gcaatcgaat ctgcttctgc tcggggggcg ggaagacggc gatgatacgc
     9241 tgaattgttt ccgtcgcgtc gagtgtatgc aaagtagaga acaccaggtg accggtttcg
     9301 gccgcgagca atgccgtggc aatcgtttcc aggtcgcgca tttcgccgac cagaatgacg
     9361 tcaggatcct gacgaagccc ggcgcgcagc gcggttgaaa aatttgccgt atcgacttcg
     9421 acttcgcgtt ggttgataaa actttttttg tcgcggtgca ggaattcgat aggatcttcg
     9481 atggtgatga cgtgatcgga gcgcgtggta ttgatgcggt cgatcatcgc agcaagcgtc
     9541 gtcgacttgc ccgaaccggt agttcccgtt acgagaacag gcccgcgctg ctcttcgcaa
     9601 atcgtttcga caacgtgcgg cagattcagt tcttcaatgg tgcgaatctt ggtgggaatg
     9661 acgcgcagca ccatgccgac gtttccgcgc tgctgaaaaa cgttgacacg gaagcggccc
     9721 agaccggcga caccgtaggc catgtccaat tccgccgttt ccttgaactt ttgcttctgg
     9781 cgattggtca tcatgctgaa ggccatggac aacatttctt cgggcgtaac gcgcgggacg
     9841 tcggtcagcg gcgtgagcga cccatcgatg cgcaggcagg gatgattgcc gactttgaga
     9901 tggagatcgg aagccttggt ttcaacggcg cggcgcagaa ggtcatcaat gctgagtgcc
     9961 atggttagcc tgtctccacg tggaaagtcg cagcatcgaa cgtagcattg ggtaaacggg
    10021 cgggcaagcc tgcaggatgg gagtgattct gcctagtaac ggtcaagata tatattgcag
    10081 tttgttatag ttagcaggct tgtgcgcggt tcagttttag cctaattcgg ctagtgcggc
    10141 ttccatttcc gatcgcgcgt gatcgtcgcc tatgcgctgg gctaccgcga tgccggaggt
    10201 cagagctgct ctcgcttcat ttgtgcgtcc ggacttagcg aggaactgtc cgtattggaa
    10261 ataagcggag acgtagtcgg gatgcgatgt cattagcgcg ttgaaatttt cgtcggcagc
    10321 ggcggcgtcg ccggtattcg agcactccat cgccaagccg taacgggcga aggcgtccgt
    10381 gggctttgag gcaacgaatt cttcgagcat ctggcgacgt gttttcttcg gtggttggga
    10441 caatgtcatg cgcatagtat attcgcgctg ctgcggaacg ccaaatcggt tgtgggcagt
    10501 cggctgcagc caccataaaa aaaggcgatt tcttactttc gggagcacaa gtccattgat
    10561 taaaaaaact tccagaaatt caacgaaaga gaaaaaacag tctgaaaaag tgcttcccgg
    10621 acggctggtc agcgcttcgc gcaccgaaat gacggaactg gtgctgccgc aggactcaaa
    10681 cttgcggggg aatattctgg gcgggcgagt catgcacatg atcgatattg ccggggccat
    10741 tgctgcgcac cggtattgcc accggcaggt ggtcacggcg tcggtggatc atctcgattt
    10801 cttgaatcct gtgcgcgtag gagatttaat tgtgctggaa gcgcaagtca atcgggcgtt
    10861 tcatacttcc gtggaagtgg gcgtcgaagt tttttctgaa gattccgccg tcggaattcg
    10921 caaacacacg acgacagcgt ttctgacttt tgtggcgctg gatgaagcgg ggaagccggt
    10981 tcccgttccg ccggtgattg cgaaatcgag cgaagagaag aggcgttaca aagaagctgg
    11041 agaacgccgc gaattgcgaa tcaaggcacg gaagaaaaat aaatagtttc ttttgtttct
    11101 ataggttttt tcttttcacg cctttctctt tttttcctcc gtgtcctctg tgtgaaagtt
    11161 tttgaatttt cacgcgaatc tcgaaccgca aaatccttta cctggaggac acagagagga
    11221 cgaagaattt aattacttac tttggcgatc cacgcgatag gcgtggtggg ttgagggctg
    11281 ccgccggcgg gctcatcggt tacggcgagc gcggcaattt cattcaggga aattgtgacc
    11341 ggagcgacga atgcaatctg cccggtgctg tcgggatgga agatcgcggc gctcacgggc
    11401 tttccggatt tcggcacaaa ccaaagttgc agggtgcgcg ccgaaggcat ggaaggcatc
    11461 tgatccgctg taattgccac tcccatgtgc gggtgcatat acgcatgcac gaccggcatg
    11521 tttttatccg cggactccag tttcaattgc ctggactcgg gagaaagcat cacggaggcg
    11581 accatgcggt ccatctccaa ttgattttgg agagcctggg tttgcatggt cgcaattttc
    11641 atttgcttgc gaagttcggc aagctggtcg gtttggcggc tcatctgccg aatcgtgtag
    11701 ccagtgatga gcgcgagcgc cgcggctgcg agccatgccc acgccgggaa caatgcgcgt
    11761 gggggagtaa ctgctttttg ctcgatacgt ctggtcgcgc gcgcggcgtc gcccgacgcg
    11821 ttgaccgcgt ccatgatttt ggctcgcagc tcggacggag gctcaacgct cggtgctgtg
    11881 accgcaagtt gagcaatgaa acctctcgct tcggctacgc caggggcgca cttcggacat
    11941 gctcgagcca agtgctgctc gagttccgcg cgctcttcac catccagcac accaagcgcg
    12001 tactcatcgt aaagcagtgc gagttgttcg caagtcatca agagcttgct tctccaagtg
    12061 actgccgaag gcgcaacagt ccagtgcgaa tccagctttt taccgtgccg agcgcttctc
    12121 cggtggaagc ggcaatttca ctgtgcgaaa ggccttcgaa gaacgccagt tctattgctt
    12181 tacgctgcgc atcgggaaga gaggtcataa gagcgcgaat tttttccgcg cgcctcgagc
    12241 ggtcaatctc gccttccggg tcagaacgca ccacggcaga aggcagaata tcggaatccg
    12301 aatcttcgcg gcgccgttgt ttttcgcgct tcagtcggag gaaatccagc gcgcggttac
    12361 gggccatggt aaatagccac ggttcgagag ggccgcgttc aatctggaaa cgctcggcgc
    12421 tgcgccacag ctgcagaaaa acatcctgta caatttcttc ggccgaagag gggtgctgag
    12481 cgatgcgcag aacaagagaa tatacgggcc tgccgtagcg gtcatagagt gactcgagcg
    12541 cgccggaatc gccctgcagg atacgctcca ttaacgcagc gtccgtgagc tgtgtgccct
    12601 caggccaggg ttggacgacc gcgaaaagca aaattcaaca tcctttcggc tcttctatca
    12661 atacgtagaa accagggaat tggatgaaag cacactccgc gcgattctgg caagcaactt
    12721 aactacgcga aatggtggtt ttagtaggat gggcctcagc ctgtcttctt ttgattttcg
    12781 ctgcgtccgc agacgtcaac gccggacagg gtgaagcctg tcctaccaaa atcacgcatg
    12841 cgcgacagcc cctaccccgg cttctccgat tgaggccaga taaattcgcg ccgcgtcggc
    12901 ggtacggaag gcttgttcta cgcttgagcc gatggatgga ccggacaaat aatttcccgt
    12961 aagaaatagt ccaggtatgg agcctgccag cgtttcgagc gattttacta attgcgtatg
    13021 tcccaaattg tattgtggca acgcgtgcgc gtaacgttgc agatttgtgg tcaccggttg
    13081 atctttgatt cccaggacgc gcgcgacttc gccgcaaata gtttccgtaa tttcccgctc
    13141 gctcaattca caaaatttgg gatcggtagc accgcctgaa aaactggtca gggaaaccat
    13201 tccctccggc gcgcgcccgg ggaaaagcga agagttgaag accgttccca gtgcatggag
    13261 tccttcggag cggggaacaa gaaaaccgaa accatcagga ggtggctgaa actgctcgcg
    13321 gcgatagccc gcagaaagga cagctaccgg cgcgtattcg atttgcgaaa aaatgggcgc
    13381 gaacgattcg gaaagattgc gcagaatttg cgaagtgact tgcgtgggcg tggcgaagat
    13441 tacagcactg gcggccaacg tttcgtgttg gtcgcggcgc gcaatatcga tttcaaacca
    13501 gggtttgccg ttcgctttgc catggcgaac cgcagtaacg ctcgtttcaa agtgcagcga
    13561 ggagaccagt cgtgcggcga tcgctcgcgg caaagtttcc atgccgtcac gaaatgagca
    13621 gagttcggcg cgtggagtgc cttttgccgg acgcgacttt atggcgccgc gaagtacgct
    13681 tccatattgc gattcaaatt cgtgaatctt gggaaacgag gcacgcatac tcagccgttc
    13741 cgggtctccc gcataaactc cggacacaaa tggcgttacc aatctctcca gtaattccgg
    13801 cccgaacttg cggcgaacga aatcggcgat ggattcatct tcggctggcg gctgcgtcct
    13861 gcgcatgatt tcggtgagca gccgccactt cgtgccgccg gaaaacaggg gcgtagaaaa
    13921 aagggaaggc ggagacatcg gcgccggaac catctttccg tgcaagagaa tgtagcgcgg
    13981 ggctcgcgga tcagcatgca acagctgatc gttcaggccg agcgcattga tcagcttgag
    14041 cagcggttcg gtggtgagaa aactttgcgg accgatgtca aagcggaacc aatcttttcc
    14101 tttggtggcg atgacgccgc cagcgcgcgc gccagcctca acggcgtgca ctggaattcc
    14161 agcttgctgc aaatgatagg cgcacgcaag gcccgatatt cctccgccaa tgacgagtac
    14221 gggatacgca gtctgcgcgg aagtggacac taggaaagcc tcgatcgttc gatcagagag
    14281 tttttgaaaa gagtgggcgg ctttcgagct tctgtttctc gagatacgga tcgaacacca
    14341 tggcgatatt gcgaatgaat atgcggccca gcagcgttgt gcgcagaacg ccgttttcaa
    14401 gttcgattaa accgtcggac acgaagggag cgagacgctc gatttccgga gcaaatgtct
    14461 cattaaaatt aattccaaat tcctgctcaa tttcactgtt gatcagcacg ccgtggcaca
    14521 agacgcgatt gatgacggtg cggcgcagta aatcgtcttc ggtgaggcgg tagccgcgca
    14581 tcgtcgcgat gccgcgactt tcgacgcgct cgcgatagtg ggtcacgtcg cgatcgtttt
    14641 gcgcgtaaca catgctcagt ccgctgatgg cgctgacgcc catgccgtag aggtcggcgc
    14701 cggctttcgt tgtgtagcct tgaaaatttc tgtgcagcgt tcgctcgcgt tgggcgacag
    14761 caagttcgtc attggggcgg gcgaaatgat ccatcccaat gtattggtac cccgcgttta
    14821 aaaaactttc gaggcctgcg cgaaaaatgt ggaatttttc catgccttcc ggaagccgcg
    14881 tggcgagagc gccttgctgc ttgcgaagcc acggcacatg tgcgtagctg aacatggcga
    14941 ctcggtccgg cgcgatggcc acaacttgtt ctaccgtgtg ggcgaaacgc tccgccgttt
    15001 gcaatggcag gccgtagatg aggtcaacat tgatgctgtc aaaacccagt tttcgagcgg
    15061 actcaatcag gctgcgagtc atttccagcg gctgcacgcg gtgaattgcc agctgcactt
    15121 caggatgaaa atcctgaatg cccatgctca ggcgattgaa gccaagccgt cggagcgttt
    15181 ccaaatgagc ttgcgtagtg acgcgaggat cgatctcgat tccgatttcg gcgtcgggtg
    15241 cgaacgtgaa tcgttccgcc gtgaagcgga aaaggccttc catttgttcg ggagtcagat
    15301 acgttggcgt gccaccgccc cagtgaaatt gcactacggg ccgctcgcgt gacacgaagc
    15361 ggctgacgtg ctcaatttcg cgctcgagcg tttcaatgta cggtggagcg acgctgtgat
    15421 ctttagtgat gaccacgttg caggcgcaga acaggcacag gctctcacag aacggaagat
    15481 gcatgtacaa cgaaaccgga gtgcgggcgg cgttggccgt tgcgtaaaat tgctcgaggt
    15541 catccgggcc gaaattatcc tgccacacgg gcgcagtggg atagctcgtg tagcgcggcc
    15601 cgggccggtt atatttctcg agaaactctt cggaaatttc gaagcgttcc cggttcagcc
    15661 ggagatgaag atttgaatcg ctcatagtgt aaacgggcga aaagtgccgt ctccgcccca
    15721 aaaaaattcc agcagcaact tcagattgcg tcttccgcgc gcacttcgtc cccaacagag
    15781 atggactgac cctcttgaac aaaggcgcac gcgttttcta ccggcgtttg ctgcaaaatt
    15841 ccgtgaccca aattgaggat gtggccgacg ccgcgggaac tttcgatggc ggcgcgcgcg
    15901 gccttgcgga ctcctgcttc cggtccaagc agcatacatg gatcgacgtt gccttgcagc
    15961 gcgatacccg gcccgacttg ttcgcgcacc gttgcaagat cgacgcgcca atccacgctc
    16021 aatacattcg cgccagattc ggcggctgca gtaagcaggt ggttcgacgc cttggagtag
    16081 agaatgacag gcgtatctcc cgcgtcaatt tctgcgatca gcagttgagt cgcgggcaat
    16141 gcaaattcgc gatattggtc gacgttcagt tcgccgaccc acgtgtcgaa caattgcacg
    16201 gctgaagcgc ccgccgcgat ttgcgcccgc aggtagactg ccgtagcgtg cgctatgcgt
    16261 tcaagcaaat tgcgaaacag ttcgggctcg gaatagagca gcgtttttgc agcgggaaag
    16321 ccatctttgt ggtggccctc aatcatgtag caggccagtg tccacggcgc tgcagcgaag
    16381 cccagaactg gcacggtagg tccaagctcg cgcacgaggc cgcgaatggc attcatcagg
    16441 aagcctgtgt cccgctcagg atcgaaggaa gatagctcac gaatctgagc cgccgttcta
    16501 agcggaggct cgatcacagg acctttgtcc gacaattcga acggcaggcc cattgccccg
    16561 acggggatta agatgtcgga gaagacgatg acggcgtcca cgcccagaat gcggtgaggc
    16621 tggagcgaaa cttctatcgc taattctggg gtcttgcaaa cttccaggaa attatgtttc
    16681 tggcggatgg cgcggtattc cggcaggtag cggccagcct ggcgcatgat ccagacaggg
    16741 acgcgctcta cgggttcgca acggcatgcg cgcaggaaga gcgttttctg gctcacggat
    16801 tcagcaaatt cggtcattgg cgtcaggtcg cgtattcatc ataacaaaag tccgtgttgc
    16861 ctgtatgctg cggatagctg catgttttcg tgaagcgatt acctgcggga atcgctcaaa
    16921 ctgtggggca agagaatcgg cacgatcacc taagaatagt cttgccgatt ttgtggggcc
    16981 gtgagaaggc ccagggatac aatacatagc aggtccaagc aggaaaaatt cccatgaatg
    17041 gagtcaacgc caatatctgc gagggtgttt ttagccccag ataggcgaag ctgagccccg
    17101 gcccgaccaa tgcgccaaca accaaccata cccatctccg cttcggacca aggatgcgaa
    17161 ccacgattgt aaggaccagt gaaaaaagaa atgcgggtat gctgaggaag ggactataca
    17221 tgataatgac gaaaagaatt cccgctcctc cgcttggtcg ctggcctccc aaaaaagcaa
    17281 ccgtgaacgc ggcgcttgcg attgtggccg ttacagctgc aaggaaatag gccaccacgc
    17341 acatcactcg atccgctaga ttgtgcacgt cggcaagaat gcggcgccac ggaaccttat
    17401 caagccaaag tgtcctctcc agccaccgtg tcgccaccat ccacggtatc gtcttgccca
    17461 gatgactttt cgggccaaag aactgagtgc gtacgggctt ctgcagtaaa accgcgaggg
    17521 cagcaaactt ccaaacattg acctccagga agtcgggtcc ccgaaccaga ccgacgccat
    17581 tttgaaagag ggcttgcgcg cacatgccgc catccgcaat gactgccagg atccatcccc
    17641 atcgctgtat gcgccaaatg ccaaatagcg aaactccggg tagtcctgcg tatatcgaca
    17701 aatgaattgc gagcggcagc aaactcgttc cgtaataagg atgcagcgac caggataaaa
    17761 gcgttccaaa tcccggcaca acactcaaaa acgtcaggaa ttgaagtatg agcgttgcca
    17821 acagccaacc cggccgcacg cccgggatcg tgttgatctc caagaatcca ctaacctcgt
    17881 ttccagtgtg caaaccccgt tgaacggatc gatttattca gtctgggcca ggtcgatttc
    17941 ggagtctagt ggcaattcag tgggccaaac gtaagttgca attggcgctc gcgccgctat
    18001 cgttttgggg caaacctgcc gctatgtatt tgggatttgc gagaggcgtc gcctgcggtc
    18061 gagattttgc cctgctgcgg tcccagcata atcactcact cgatggctac gcgcgtgagc
    18121 cgccttcaaa tgggcgggag ttatctggtt actgacgctg tcagaacttc agcatcgtgc
    18181 tacgcgtcag agtaacctgg ctggtcactc ctgccggagc gcctttgtac agatgatagg
    18241 ctgaatactg ctccatgtaa ggatcgtcgg aagacaggca atcgattgga ataatcacct
    18301 tatagccgcg ctgcgcggag ccaccgcctg tgccgattcc cacgccttga aatgaagttc
    18361 cgcagacaat cactgtttta attcctcgcg ccttcaattt ttcatccaaa ttcgagccga
    18421 taaatttgtc aggtccgttc tggcggaccc attcgccgtc acgcgccata acgcccggat
    18481 caatctgatc ggctggcgtg gcttggccgt cgctgccgac gtagctgtac caaaccaatg
    18541 ctccagcagc gcgggctgca tcatgcaatt tctttacatt ggggaccgta gccacgcagc
    18601 gcgggcgtgc accgcaattc attttcatca tatccagaat caacagagcg gtcgtcgccc
    18661 catcgagcgt aacggatttc agctgcggca atggcggagg ttttactgcc gaccaatcgt
    18721 cgaggatgtc agcagagagc ggcgcggccg agaagatgag tgctgatacg acgacaaagg
    18781 ctgcgaggcg caaaagtttt gttttgagtg acataattca cctcttcgaa agatttgtac
    18841 tctcgctgtt cataaacgaa aagcttaggc ctactttctg aggcatccta cgctttctgg
    18901 ttctatcaga caatgaattt gcaattgcac tacgcaagtg agaagtccat tcccacaatt
    18961 tttcctgtgc gctggtactt tcatgctcca gaattagcta taataccgag gcaacggtga
    19021 tttgcggcag gggggaacac ctgccagcga gtgcctgcac aaaatgacaa gtccttacgt
    19081 aattgcgacg ctatttttat atgcggccag cctggcgctc tatatctgga atttgcgcga
    19141 accctcggcc tctgtgggaa ttggcgcaac cacctgcctc ataggaggct tgggacttca
    19201 ttaccttgcg ctcctggcgc gctcgcggtt gattcacgcg gttccttatg acgatctttt
    19261 tggctcactt tccctttttg cgtggctact cgccgccacc tatctttgcc tcgaagccat
    19321 tcaccggcgc cgcgtggttg gcccgttcgt attgccgatt gtgctggccg tatttctgct
    19381 tgctcatgtg cctgaggcca agctggtaaa agcaccgcct gcgcaaggtc cgctgtttgc
    19441 atttcatgtg acgctgaaca ttctggccta ttccgcattt gcgatttctt ttgtgctcag
    19501 cattattttt ctaattcaga accgtcgctt gcgcgatcac aagctgggtg atgtgggatg
    19561 gcgctttccc gcgcttgacg ttctcgatcg catgacgcgc agcagcgttc tgatcggatg
    19621 catttcgctg gcggcgggta tgacggcggg aatgatctgg gcgcatcgct tggaagggcg
    19681 ctactgggat ggcgacccga aggtgatcgt tacggtagtg attttgttgg cctatgttgg
    19741 ctacacattt ttgtccagga cgtcagcttg gcggggggcg cgcgcgtcgg cgctctgcat
    19801 attcaatttc atgttcgtaa tttttagtta cagcatcgtg aatcgttatc tttctcattt
    19861 tcaccggtat ttttgatctg gataaagtgt agggatcgga gaaagctgtt caggcgattg
    19921 ctagatggaa attgttctca tcggattgaa ccataagacg gcttcggtcg atctgaggga
    19981 gcgcgtcgct ttcacgccgg accaagcgcg cgaggccgcg gagcaattgc gttcgcgcgg
    20041 aatcctcgaa gagtcgctgg tgctttccac ctgcaaccgc agcgagctgt atggagtgcc
    20101 gagcgaatcc actgcgaata gtgcgggtgc cctggaatta ttcatcgctt cgtttcatca
    20161 gctttcccct agcgatttgg accattcgct ttaccggcac agggatagcc aggccgtgcg
    20221 tcatttattc cgcgtttcgg ccggactcga ttccatgttg ctgggggaag cggaaatttt
    20281 gggccaggta cgggatgctt atcagatcgc gctggatcgc ggagaaactg ggcccgtgct
    20341 caaccgcatg tttcaagcgg ctctggaagt gggcaagcgc gttcgcaatg aaacagaact
    20401 tggcacgcga cctgtttccg tggctttcgc cagcgtgaaa ttggccgagc gtattttcgg
    20461 caagctgaac acgcatcgtg cgctcatcgt tggagcgggc acgaccagcg agcaggttgt
    20521 aaaacatctg tgcgaccgcg gcatcaagca gctgcgcgtc attaaccgca cgccggaaca
    20581 tgccgtggat cttgctgcac gattcgacgg cgaggttgtg ccgtgggaga acttgtcggc
    20641 ggcgctggaa tggccggact tgattgtgac atccgtatca tcgacggagc ccattttgtc
    20701 tcgggcaatg atcaataagg cgatggccgc acgcggaaat cgggcgctgc tcatgattga
    20761 tcttggagtt cctcgcaacg tcgcgccgga tgtcgcggat atttataata cgtatttgta
    20821 taacatcgac gacctgacag aaattgtgga acagaataag aaagtgcgcg ttgctgaaat
    20881 tccccgagcc gaagctatca tcgacgagca ggtggataaa tttctgcact ggcaggctgg
    20941 cgttgccgcc ggcgcggtgc tgggcgatct gaaaagtaaa ttggccgcgg agcgcgaagc
    21001 ctttatgaaa gagcggttcg cggcgatgcc caatctttcc gatgaggacc gcgcgcaagt
    21061 ggccgcgatg atgcaggaat ttacggagcg catgctcatt acccccgcgg aaaaaatgcg
    21121 cggcattcct gaattgcgcc gcaagcttca aaattttgaa gcgattcgag atttgtttca
    21181 tctcgaccgg gagaagccgt gaaggcattg cggattggca cgcgaggcag cgcgctggca
    21241 ctatggcaat cgcgaagtat tgcgcatgcg ctggccgatc gtacgggcgt ggagcctgat
    21301 ctcatcatca tcaaaacctc cggtgataaa tttcaaaaac tcagctttag ccagattgga
    21361 accaagggcg tcttcatcaa ggaactggaa gatgcgctga tcgaggaacg catcgatatc
    21421 gccgttcaca gcatgaaaga tgttccaaca gaattgcccg aaggtttgac gattgcagcg
    21481 attggcaaaa gggaagacgt gcgcgatgcg ctgctctctt tgaacggcgg aactctggct
    21541 tcgttgccac aaggtgcgcg agttggcacg agcagcttgc gccgtcagtc gcaattgctc
    21601 gcagcgcggc cggatttgcg catgctggaa ttgcgcggaa atgtggacac acgcatcgag
    21661 aagctgaagc gcggaagtta cgacgcaatc gttttggcaa aggcggggct tgaccggctc
    21721 ggcttgagcg caaatatcag cgaagtgttg tcttacgaaa taagtttgcc tgcagctggg
    21781 cagggcgcga tcggaattga agcgagatca gcggacgcgg gtactctgcg tgcattggct
    21841 gggctcgagg acgcggaaac tcgcgccgct gtgactgcgg aacgcgccgc gctcgcaggt
    21901 ctcggcggag gttgccaggt gccgattggc gcgtggggcc gaatggaaaa tgggaaattg
    21961 ttgctggacg tcgccgtact ttctcccgat ggaacacaac gcttggtaga gaaggacgcg
    22021 ggagcgcggg gtgaggccga ggccattggc aggcgcgtaa cccagaaatt acgcgagcgc
    22081 ggcgcaacgg cgttgctgga gcgcgaaaac cgtgggacgc cggcatgaat gtctcgagcc
    22141 cgctggatgg aaagaaaatt gttctgacgc gcgcagtggg gcagactcgt gaactgaaag
    22201 cgcggctcga aaaaatgggc gccgtcgttt tattatttcc cgccgtaaat ttttcggagc
    22261 ccacggatac aaaagagctg gatcaagcga taaactccct gaacgaattt gactggatat
    22321 tatttacgag tgcgaatgct gtgcgcttct tcgcggggcg ctgtcgcaaa ctcgaagtcg
    22381 agccgagcca tgacgtgagg tttcgctgcg cggccgtagg gcctgcaacg gcgagcgccg
    22441 ttgcggccga aggttttcca gtggaccatg tggcgcagga atttatcggc tccgcactgg
    22501 cgcgcgaact ttcgtctcta ttgcaaggcg ggaaagttct tttgccgcgc agcgagcggg
    22561 cacgtcccga tttacccgcc gcgttaagat ccgtcggcgc gctggtcacg gaaattgtgg
    22621 cgtatcatac cgggggcgtt ggcacgatcg atccggtcgt gatgagcgct attcgcgagg
    22681 ctgatgtgga cgtaatttcg tttttcagtc catccgcgat agaaaatatg cgcgttgagc
    22741 ttggcgagga gttgctgtct cgccttggtg cgaaagcaaa aatggcggcg gtcgggccta
    22801 caaccgcggc ggcgttgcga ggcgcgggcc tacctgtggc tattcaggct ccgcttgcaa
    22861 cggcggaatc catggctgta gcaatcgcaa cttatttctc cacgaataca ggatcaaagg
    22921 cgagggccat atgaattttc cggtaacacg cccgcggcgc ttgcggcgca acgaagcgat
    22981 acgttccctc gcgcgcgaaa atcggctatc tactcagggc ttcatttatc cgatgttcgt
    23041 ttgtcccggc aaaaacgtgc ggaatccgat ttcttcgatg cctggcgttg cgcagcagtc
    23101 ggtcgacatg cttgtcgagg aatgccgcga agtggagcag cttgggatac cgggaattat
    23161 tcttttcggc atccccgata agaaagacgc gctgggcagc gaagcctacg ctgcggacgg
    23221 aattgtgcag cgcgcaattg agggaattcg gaaggccaat ttaaatttgc tggtgatgac
    23281 cgacgtgtgc ctctgcgagt acacggatca cggccattgc ggaattatcg aaaacgggga
    23341 agtgaagaac gacccaacgc tggagttgct ggcgaaggaa tcggtttcgc acgctcggtg
    23401 tggtgcagac attgttgcgc cttcggacat gatggatgga cgcgtcggtg caattcgtaa
    23461 ggctctcgat aatgcgggat ttcaagatgt ggccatcatg agttacgcgg cgaaatactg
    23521 ttccggattt tatgggccat tccgcgaagc cgcggaatcc actccacaat tcggggatcg
    23581 gcgcggctat caaatggatc ctgccaacgc tcgcgaagcg cttcgcgaag tggaattgga
    23641 cttggcggaa ggcgcggaca tggtgatggt caagcccgcg ttggcttatc tcgacattat
    23701 tcaacgcgtg cgcgaaacat tcagtgtgcc ggtaggcgcg tacaatgtga gcggcgaatt
    23761 cgcgatggtc aaggcggctg cggcgaacgg ttggattgat gaaaaaagaa tcgcaatgga
    23821 aattttgacc ggcattcacc gcgcgggcgc gagcatcatt cttacgtatc atgcgaagga
    23881 cgcggcgcgt tggctaaaaa attcctaaca tccactcctc actcaatagg cggcaatgag
    23941 cgcacctaac ccaacaaaat tgcgacgtcc gcactctctg gaaatatttc accgtgccac
    24001 cgatgttttg gtcggcgggg tcaacagccc tgttcgcgcg tttcgcgccg tgggcggcga
    24061 accgattgtg gttgaccgcg ccgcgggcgc gcgcctttgg gatgcggatg gaaatgagta
    24121 catcgattac gtgtgctcgt ggggggcctt gatcttaggc cacacgcacc cgaaggtcgt
    24181 tcaggccatc tcggatcagg ctcgccgcgg cactagttac ggaatgccca ccgaacttga
    24241 agtcgaactc gcaacccgca ttcgcaaggc gcttccttcg tgcgaaaaag tgcgctttgt
    24301 aagttccggc accgaagcca cgatgagcgc agtgcgcctc gcgcgcgccg ctaccaatcg
    24361 agatctgata gttaaatttg acgggtgcta tcacggccac tccgatagtt ttctttccga
    24421 ggcaggctcc ggccttgcaa cgcttggaat cgcggcctgt cccggcgtgc cgcaagcgct
    24481 cgcggaattg acgctgaatg cgcagtataa cgacgctgcc gcggttgaaa aacttttcga
    24541 tttgcaccct ggcaaaattg gcgcggtgat tgtcgagccg gttgcggcaa acatgggcgt
    24601 cgtcgcgcca aagcctgaat ttctcaaagc tcttcgcgaa attacgtcgc ggcacggagc
    24661 tgtgctgatt ttcgatgagg tgattaccgg ttttcgcctt tgctacggag gcgcgcaaat
    24721 cgttttcgga attactccag atttgacgac gctcggcaaa attatcggcg ggggattgcc
    24781 agtagccgcc tatggtggcc gccgcgattt aatggaccgc gttgcgccgc ttggtcctgt
    24841 ctaccaggcg ggaacgctgt ccggcaatcc gttggcgatg agcgctggaa ttgcttcgct
    24901 tgacctgctg gccacgccgg gattttacga agcgcttgac gcgcgcgcga aacgtttggg
    24961 agacggcatg gttgcagcat tgcgcgaaac gggcactccg gctacggcgg ttcgtgtcgg
    25021 ttcgctgctc acggtttttt tctcgcgtga agagatcacg gattacgccg cggcgaaaaa
    25081 gtgcgatacg cgccgttttg ctgacttttt tcgcggcatg ctcgatcgcg gaattttcat
    25141 cgcgccttcg cagtatgaag cgttgtttgt ttccgccgcg catacggatg cggatatcga
    25201 tcgcacgatt gcggcatttc gcgagagcct gagcgcggtg gccgaataat gtagcgccgg
    25261 cattctgccg gcgggtttgc gctcggttaa tctgacttaa aaccgcgggc gctacaaaaa
    25321 caaggagcag gaattcatgg ctgacgaatt aagcgaactg cgcgagaacg cggaacacgg
    25381 gcgggaaaat ccatcgtttg ttcacgtttc ggtgacgatg gcgattctcg ctgtttgcgt
    25441 ggcgatcgtt tcactgatgg ggcatcggtc gcacaccgaa gaactgatga tgcagaatcg
    25501 cgcgaccgat cagtgggcct actaccaggc caaaaatatt cgactgcata actatcaaat
    25561 gagcatcgat ttactttcca tgattgaatt caagaataag gatcaagccg cgaaagttca
    25621 cgataaatat cagagtgaag ttgaacgcta taccaaggaa cagacggaga tcgaggagaa
    25681 agccaaagaa gacgaaaacg agtcggccag cgcgcaacat aaggcggacc ggtacgacct
    25741 gggcgaagtg tttcttgaaa ttgcgctcgt gatttcctcg cttgctctgc tttcaggcaa
    25801 gcgcatcttc tgggttttgg gactcatgtc cgggctcacg ggcttgggca tcgccgcctc
    25861 ggtttttctg ctgcattagc caccgcagag gttttgtggc tcaggccgga tgccggcgcc
    25921 acaaaacctc aaatcccgcg cggtgggtgt cgaaaatcaa catcaagaac tgggcggagc
    25981 ggggcgcagt cttagcgttg cgatttcttc cgtcagtcgg tccagccgct gcatcagttg
    26041 tctgtgacgc tcctcttccg gagccggctt tttgccggtg aaacaaattg tgccgcccgg
    26101 atcgagcgtt gcgcgatcca cttcctgcaa agttccgaat cactgtttgt gcgccgccgt
    26161 ttgaaggtcc tgaaccgaga gtaattcctt cgtgagcctg tcttccagaa tcacgccatt
    26221 ctcgatgagg atggatgtgt ccccttcaac aaagcggtcg attttttcgt ggcgaaatag
    26281 gaaacgaaca accaagtagt tcaagagcag gagcgttgcc gcgccgatga ttcctccggt
    26341 cacgctgttg tcgtctccga tgattgcgtt ttgcacggta ttggagagcg tcaggagaac
    26401 gaccaggtcg aaaggattca gctgcgccaa ttctcgtttt cccgcgaggc gcaatccaag
    26461 cacaagaaaa acataaacga cgatgggtcg caaaattttc tcggctacgg gaatggcgag
    26521 tacaaacatg tgattcatga gtgtggaatt caatttattt ctcctgatgc ctgaaaattg
    26581 atcggcgcgt tgaataataa agccggattg tttcggccaa attatcgcct cgcgcagggt
    26641 aacacgttcg ggaatttcgt tggggcttgg ctgggatttt gcacggccac agaaatgttg
    26701 gagtaggacc acaaaaagct gcccgccctc aaaacgggcg tgcgctacga aggccggttc
    26761 ctacgggcgt ctcttgacgc acagcgacgg ctcttgtata ttcttttgcg tcatgaggtt
    26821 ttccgcgaac gatcacggtc atcatcacaa tcacgtcgaa agaccgtgcc cgcggaggct
    26881 tgagtagcgc aacgaaattc gcactcatag acccgctggc gcggtaaacg ccagcgggtt
    26941 ttttatttta cggcaacttt tgtcaggtcg gggctttggg aggagtgatg gatctggatt
    27001 tcaagaaact ggatggttta attcctgcgg tgattcagga tgccacgtcg ggtgaagtgc
    27061 tgatgctggg cttcatgaac tccgaagcct atgccgcgac gcaggccatt ggggaagtaa
    27121 cgttttttag ccgctcgcgc aataaaatgt ggcgtaaggg cgaacagagc gggcatgtgc
    27181 tgcgcgtacg cgaaatactg gtggattgcg atgaggacac gctgttgatc cgagtcgacg
    27241 cggttggccc tggcgtttgc cacgaaggct accgcacttg ttttttccgg cagatcactc
    27301 cgtctggcga agagaaaatt gtcggacagc gtacgttcga tccgaacgca gtgtacggaa
    27361 aggatgcaaa ataatgggcg tactccggct gggcattccc aagggcagcc tgcaggatgc
    27421 gacggtgcag ttgtttggaa aagctggctg gcgcatttcg atcaactcac gcagctattt
    27481 tcccggcatc gacgatttgg aaattaattg catgctcgtg cgcgcgcaag aaatggcgcg
    27541 ttatgtagag accggagcgc tcgacgccgg aatcacaggg cgcgattggg tgtttgaaac
    27601 aggctcgaag gtcactgaga tcggcgagat gatgtattcc aaacaaagcc tggcgcctgt
    27661 gcgctgggtg ctcgccgtgc cggaagatgg tcctattcaa tccgtgaaag accttgcggg
    27721 aaaagtgatt gccacggaag tggtgaatct cacgaagcaa taccttgcac gcaatgatgt
    27781 agaggcgcgc gtcgagtttt cctggggcgc tacggaagtg aaggtgccgc agcttgccga
    27841 cgccatcgtt gaagtcacgg aaacgggctc ttcgctgcgc gcgaatcacc tgcgcattgt
    27901 cgatactatt cttgaatcgc gcaccgtgtt tatcgccaat cactctgccg cggccgatcc
    27961 gtggaaagct gaaaaaattt ccaatatttt tctgctgctt tcgggcgcga ttgccgcgta
    28021 cacgaaagtt ggcatcatgc tgaacgcgcg caaggagcaa ctcgctgctg tgctggcggt
    28081 gttgcccgcg ctcaagaatc caactgtgtc cactttgagc gatcctggct gggttgcggt
    28141 caacacgatc attgatgaag acgttgtccg ctcgattatt ccgcgattga aggcggcggg
    28201 cgcgcaagga atcgtcgaat atccgctgaa taagattgtg gtgtgaagaa aagttcaaat
    28261 cagatgcgaa tactcaaatt aacggaccga gtggcggaaa aatttttcgc gcagcgcagc
    28321 tcgtacgatg cggctgccga caaagttgcc gcggagattc ttgctgatgt tcgcaagaat
    28381 ggcgatgctg cgctatttcg ctgggcgttg cacctcgata agctttcgct cacgaagaaa
    28441 tctctgtgga tgtcgcgggc ggaaattcgt gtcgcgagcc gtggcgtgtc ccgcgaactc
    28501 gtgcgcgcca ttgagcacgc cgcaaaaaat atccgccgcg tggcggaggc gcagcgtccg
    28561 cgctcttgga gcattaccgt ggaaccggga gttcgcgtcg ggcagcgagt cacgccgctt
    28621 ggaacgatag gctgctacat tcccggcggg cgattctcac ttttttcaac gttgctgatg
    28681 acggcaatcc ccgcgcaggt cgccggggtg aaacgcatcg ttgtcgcctg tccgaagccg
    28741 agccctgcgc tattcgccgc tgctgaaatt cttggcatta cggaaattgc gcggatgggc
    28801 ggagcgcagg ccatcggcgc atttgcatac gggacgcaat caattccaaa ggtcgataaa
    28861 atttgcggcc ctggcaatcg ctatgtcacg gcggcaaagc gcatcgtttc caacgattgc
    28921 gccattgaca tgcctgctgg cccgaccgag gttctgatcc ttgccacagg tggatcgccg
    28981 cttttcatcg ctgcggattt tctggcgcag gcagaacacg atcctgacgc tatcgctttg
    29041 ctgatcactc catcggcgcg actcgccaaa tccgttgcgg cagaggtcga caagcaattg
    29101 aaagatttgc cggaaacgaa tcctgcatat aaatctttgc agcgtgcctg cgcgatcctg
    29161 gttgcgccat cgctcgacgc ggccattcga tttacgaatc gtgtcgcgcc tgaacatctg
    29221 agcatccctc aggcaaacaa atcattgatc gagaaattgt ccgaagcggg aagtatattt
    29281 ctcggcccct ggagcgcgca gccgattgga gattacgcca gcgggacaaa tcatgtgttg
    29341 ccaacctccg gttgggcgca tacacgcggc ggattgagcg tcaccgattt tgtaaaatgc
    29401 tcgagcacgc aagaaatttc gtccgctgga ctgaagcgcc tcctgcctgt cgtcacggcg
    29461 atggcgcgcg ccgaaggttt ggaagcgcat gcgcgcgccg tggaggtgcg cgaatgaagc
    29521 gcaccaagcc tcaatccatc gcgattcgca aggccgtcat gggcctcgca ccctacaacg
    29581 ctccatcgga ggggcgcgcg aaaaaacttc gcctcgactt taatgaaaat accgttggct
    29641 gctctcccgt tgtcattcgt gcactttctc gcatgacggc gaaagaaatg gcggtgtatc
    29701 ccgagtatca ggagacgacc aaacgcttgg ctcgtttttt tggcgtgcgc ccggcggaga
    29761 tgcacctcac caacggcatt gacgacgcac tgcatctgat cgctgatacg tttatcaacg
    29821 agggcgattc ggttttggtc gtcgagccca cgttcgacat gtatcgattt tttgcggagc
    29881 ttgcaggagc gagcgttgtc gcgttgcgct atgacgatga gatgcgtttt cccgtcgatg
    29941 ccgtaattcg tgttctgcaa cagcctgcaa aaagatgtcc gcgcgttttg tacattgcca
    30001 acccgaataa tccaacggga acacttgtgc agcgagaaga gttgcggcgc attttgcaag
    30061 cggctgcaca caccctggtg ctcatggacg aagcttattt cgacttttcg gggcttacga
    30121 ttctttcttg gattcgccgc tactctaatt tactggtggc gcgcacattt tcaaaatccg
    30181 cggggcttgc ggcgttgcgt attggctgtc tgttcgggaa gcctgaactt ttggcagcga
    30241 tgcgccgcgc atgcacgcca tatcctgtaa attcggctgc tttggttgcg gcggaagcag
    30301 cgattcgcga cccacgtttt ctgcgcaact acacgcgcga agttttggcg agccgcgcga
    30361 tgcttgaaaa gggattggtt cgcctcggcg cgcgcattta tccgagcagc gcaaattttg
    30421 tgcttgcgga ttttggcccg ggagcacgcc gactcgttcg cgtcctggaa aaaaggggaa
    30481 tcctcgtgcg tggccgcaaa gattttccgc gcgaaggttt tattcgcatc agcgccggaa
    30541 cgcgggccga tacgcgcaaa gtgctgcgag ccatgaaagg gatactgtga aattatcgcc
    30601 caagctgatt atttttgatg tggatggcgt tcttctggac gttcacggat cgtttcacaa
    30661 atcgatcatc gacacggtcc acttttttac gaagaagcat gtcacgaccg cggggattca
    30721 cgattggaag aaccgctctg gttataacga tgactggcga ttgactacgg attgggtcgc
    30781 gtcgctgggg ccggcggttc cctatgaaga ggtgaaagag cgctttatga aattttattg
    30841 gggcacgaaa tcgaaacccg gaaatgtggc caaggaacgc tggctgatta ctcacaagcg
    30901 cctggagcgc tggtctcggc gggccgagct tgcgattttt acgggacgca cacaccgtga
    30961 attaagccat accctggtgg gcagggatgc gcatggtatt tttcgccggg ttgtcaccat
    31021 ggacgacgta aaaaaaggga agccggaccc ggagggactc cgtttgctgc tagacggcat
    31081 gccagtgaat gaagcgctgt acctcggtga caacatcgat gatgcacttt ccgcgcagca
    31141 cgcaggcgtc ccgtttcttg gcgtattgcc gcacggcagc gaggcgcaca acgcgcgcgc
    31201 caagcgtctg cgagaactag gagcgttgaa aattctgcac agcgttcgcg atctggagaa
    31261 attgtggaaa tgagaaaagc caagcttcgc cgaaccacta aggaaacgga tattcgcgtc
    31321 agcctgaatc tcgatggaca aggccgcgcg aaaatcgcga caggcattcc gtttctcgat
    31381 cacatgctca ctctcgttgc gcgtcacgga gcgatggact tggaacttgc ggcgcgcggc
    31441 gatctggaag tcgatcagca tcacacagtg gaagacacgg gcattgtgct aggagaagcg
    31501 gtgcgtaagg cgctcgggtc gaaaaaagga atcttgcgcg ccggatattt tctgatgccg
    31561 atggacgaaa ctcttgcggc tgcggcaatc gatttcagcg gacgcccgca ccttgtctgc
    31621 aaatggaaat tttcggccaa gcatgtaggc ggcatgcaaa cagagttgct tgaagatttc
    31681 tttggcggct tcgcacaagc ggccggtgcc aacgtgcact tgagtcttct gtatggacgc
    31741 tcttcacacc atcaggtgga agcgatattt aaggcatttg cacgcgcgct gcgtttcgcg
    31801 gccacgcgcg ataaacaatt gaagaaagtt ctgcccagca cgaaaggttt gctatgaaca
    31861 tcgcactcgt ggaatacggc gccggaaatc ttccatcggt tgaacgggca cttacgcgtc
    31921 tcggtgtgga atcgcgtcgc gcgtcaacgc cggaagcact tgcggattgc gacgcactga
    31981 ttctcccagg cgtgggacat tttggtgcgc tgatgcgctc gttgggcgat cgtgggctgg
    32041 tggcaccatt gcgcgaagcc gtttcgcgcg gaactccgct gctgggaatt tgccttgggc
    32101 tgcaagcgat gttcgcgacc agcgaagaag cgcccggaga tacgggtctc gcgctgtttc
    32161 cgcaaaaagt ttccgccttg ccgcccgcgg atcgtctccc acacatggga tggaatcagt
    32221 tgcgccgcgc gcgctccagc aaattgctgg agggtgtgcc gggggacgct tatttttatt
    32281 tcgcgcattc ctatgccgcg ctcgatgcag gggaagcggg cgttgcttac tgcgatcatt
    32341 gtgcgtcgtt cgtagccgtg ctcgagaagg ggaacgtgtt tgccacgcaa tttcatccgg
    32401 aaaaatccgg gccggtagga gcgaaaatcc tttctaattt tgtgcagcat gcaggcggga
    32461 aacgatgagc cttgcgcgcc gtattattcc ctgcctcgat acggacggtg aacgcgtcgt
    32521 gaaaggcgtg aacttcgtta atttgcgcga tgccggcgat ccagccgcgc ttgccgcccg
    32581 ctacaacatt gaaggtgcgg atgaactcgt tgttctggac attgccgcgt cacgcgacca
    32641 ccgcccgacg tttctcgaaa cgattcgtcg cgtcgctgct gaattggcga tcccgctaac
    32701 agccggcgga ggcgttgcaa acgtggatga aggccgcgcg attctgcgcg ccggcgcaga
    32761 caaattgacc gtgaatactg ccgcgattcg caatcctgtc ttgctcacgg aacttgccgc
    32821 ggaattcggt tcgcaagccg tggtcctcgc aattgacgcc aaacgtgtcg gcgatcgttg
    32881 ggaagcgttc gtgcgcggcg gcagtgtgcc cacaggaatc gatgcagtcg cgtgggcgcg
    32941 ccggggagtg gattgcggcg cgggagaaat tctcatcacg tccatggaca aagacggcac
    33001 gcgctccggt ttcgactgcc cgttgaccgc cgcaatttcg cgcgcggtta cagttccggt
    33061 cattgcctca ggcggtgcgg acacgcccga aacttttctc gaagtatttg gcgagggccg
    33121 cgccgatgcg gcactcgccg cgtcaatttt tcatgacagc atccagagac ttggcgagct
    33181 taaacatttt cttggcgaac gtggcatctc ggttcgccag acgggctaat caaacacatg
    33241 atgattcctt gtatcgacct acaggatggc cgcgccgtcc agctcgtcca tggccgcaaa
    33301 ctgaaactcg cggtggacga cgtccttgga ttgttggacc gctttggcaa ccatccgcta
    33361 attcacatca ttgacctgga cgcagcaatg cggaagggaa gcaacggcag gctcgtgcgc
    33421 gggctgtgca tcgccgcaaa aaaacgcaag atgcgcgttc gcgtgggcgg cggaattcac
    33481 actgtcgcgc aagccgcaaa aattgcggcg tggggcgcgg aaaaaattat tgtaggcagc
    33541 gcggcgttta aagacggaaa cgtgaacgat aagtttctga gccgcctcgc cggcaaaatt
    33601 ggaaaacagc gcgtggtcgt ggcgctggac acggaaggcg gaaaaattct agtgcgcggt
    33661 tggcaggata aattgcgctt gcgccctgac gaggtcattc ctcaactgga aaagtatgct
    33721 tccggatttt tgtgcacgta tgtggatgcg gaaggcacta tgcgcggcac aaatctaaaa
    33781 tggttccgct cgctgcgaaa agcaacggcg cgccccatta ccgccgcggg gggcattcgc
    33841 agccgcaaag aaatccaagc cctggagcgc atgaagatgg acgccgctgt ggggatggcg
    33901 ctttatacgg gcaaactatc gtaacccgaa gctgttaaac tccgcgacag tcaagtcacg
    33961 attcccaaat cgcgcacggt cacgcagccg agtcgtccga ggatggcgta tgaatttgcc
    34021 agaactcaag atttggtctc ggaaacacaa cgcctgtttc aaccgtccgg ctatgtctcg
    34081 tccccagata gacgacgcgg caatccatct cttcttccgt atgctgattg gtgagcaata
    34141 gaagctgccc tttttgcacc ggaacgctca agagcaataa ggcgccgttg gcatttacgt
    34201 tcagggtgtg cgcttcttca tggaaagttt cttttccgga gccgtgaccg tacacgtaaa
    34261 cggaaatgtc catcggccaa cgcttgcttc tgcgccggtc agaaatttcc cccagtttca
    34321 cttctatgga gttcgattta gatttatcgg aacggttgaa ttgcgcgttt cttaaagctt
    34381 caaatattcg gctcatggtt cttcttatgg cccggctggg attaggcaat cgaaagtaaa
    34441 ttactccgaa cgcgacgcac aaaggtacgc caatcagtat cgcagttcga atgggtaacc
    34501 ccatcgggga tttcctgtat ggcgtaagtt tgtgcattgt cgccgatttt tgtagcgcgg
    34561 gcttcagacg gtggatttta gggccttgca attaccaaac ctcggcggct gaagtccacc
    34621 ccacaaaggg ctgcaaaata gcctcatcta tgagagattc gagtgagtac tgaaaggtta
    34681 tacgcgcggc gagaatgcct aggtctgcca aaatcgcgaa ggaatccgct agcactgttc
    34741 gcatcgggcc agccggatgg tcttacgccg actgggccgg aatcgtatat cccgcccgca
    34801 agccgttcgg atttcacgaa gccgcctacc tttcccattt tttcgacacg attgaaatta
    34861 atacttcgtt ctatcacccg gttcgccccg agcattgcaa aaattggatt caattggtct
    34921 ccggcaatcc gagatttttg tttacggcaa agctgtggca aaaattcacc catgaagccg
    34981 acgcgagcag ggaagacgag agcgtggttc gcgccggatt cgatgtgctc cgcgatgctc
    35041 ggaaattggg cgccgtgttg cttcagtttc cgttctcgtt tcacaagacc gaagaaaaca
    35101 ccgcatacct gagcaaattg ctgaaaaagt tttccgacta cccgctcgtg gtagaggtac
    35161 gccacgctac ctggaacgac aaagcttttt acgcgatgtt gcatgagcgt ggcgtaggct
    35221 tctgcaacat cgaccagccc gtgatcggtc actccatgaa gccaacggag cgcaccaccg
    35281 cgcgagtcgg ttatatccgc ctgcacgggc gccgctacga ttcctggttc agcgacgatc
    35341 cagctatgcc gcccttcgaa cgctacaatt atttatatag cgaagaagaa ctagaaccgt
    35401 gggctgcgcg catcaaacat gtctccgaaa gcggggagtc cactttcgtc atcactaata
    35461 atcatttcga aggaaaaggc atcgtaaacg ccctgcaact catccacctg ctcaatcaag
    35521 ccaaagtgaa aattccggaa caactccgcc agcgttatcc tgagcttgat cgcatcgcga
    35581 cggaaccgcc cgtcgagcct ctgctgtttc caaagtagtc gacaaagttt tacaacataa
    35641 aattcgtgaa cccgcctgag gtacgaaagg ctgggaactt agggccgctc cccaagattt
    35701 tgtcctgatg tgttccgcca ggactagccg gactctttgg cgctggcccg ctcggcggat
    35761 tcggattgtc cggtccgctg ggtggatttg gattgttggg cccactcggc ggagttggtg
    35821 gtgtaggact gtttggcccg ttcggcgcgg gcggattgtc gggattcgcc ggcggagtcg
    35881 gattattagg gccacttggc ggcgttggat tattcgggcc gtttggacta cccggattcg
    35941 gatcttttgg ggctggacct tttcggtcct tgggttttct cggtttagct gggccattgc
    36001 ctggaggatt cggcccgttg ggattgttcg gcggattgtt tggattattt gggtcgttgg
    36061 gagttggatt cccaggttgc tgttgattct gaacgctatc cgcatttggt tcggcggcac
    36121 tcacgaacgg agcgattgag aaagctccca tcgccagtag tgccgctgac cacccaagtt
    36181 tcttgatgtt cactgtcttg ccctccccca tttcaaccta cagagcatca cgagccggtc
    36241 caatcggggg ttatgtgtag gttaattcaa tggaaagcct taaccgtatg ttcggtgctg
    36301 gcctcgtttg aagatttctt gaatttaggc gactcgagtt tccgtcctgt cattgctttt
    36361 gcagcggccg ctcgttttat gagcgggcag cctttctgcc cgcgcaattc agaccgccca
    36421 tcatctctgc catttttcgt ctgcatatat attcacgttt gttcgcctat actgcaattt
    36481 ctcctcggtc ttgggacagg aaatgatggc tgccgatatt atttcagttc ctggcacgac
    36541 gctcgttgag tccaaggacg cagctcacgc aggagcacgg atcacccaga acgttctccg
    36601 tgaatgacat gccgtgccgc tacaaagagc gccttcgcac gatgcattcg atcccctgac
    36661 aatggtctac ggcgagctgt ttcctgccgg aattcgaaaa tccaccaaaa agaacagtta
    36721 ggaacaacct gtcctaattc tgacctaaag gacgttattt ctagggcaac cgcgtgtcgc
    36781 acaatggctg tggggagcat ctaaacatct atgggcgtaa tggaacggct tcaaaccgtg
    36841 aggcgtatgg aacgcccctg gtggctagcg gtcatagcct gcacgggagc ggttgtcatt
    36901 ggtcacttct tgcgtcagct caatatagtg ttcaatccgc tggcttggtc cagtgctgaa
    36961 atcatctctg ccttattgag ttttacgatt gccgccagcg tattggtccg atattacggc
    37021 acggggaacc gtgtttcgct tcttctgggc ttgactttcg gcgtaaccgg aatcattcac
    37081 ttaggcgcaa tttttgaagt ctacgatcat tttctgaaac attccgaaca agtccacgtt
    37141 cccgtatcct ggatgatcgg gcagacgttg cttgggcttc tcctgcttgt ggcttgcgtc
    37201 ataaataaat ggctcccgtg gccgcgcgac cctggaaaaa actttatagc attgtcatcc
    37261 atcgtggccg gggtcacttg tctggtaacg gcaatattcc tgatttttct gagcactcca
    37321 cccatccatc cgcatagccc caccccgcgc gtctgggaac ttttccccgc ggttattttt
    37381 ctaattgccg cggtcgctct gcatagcgtg aaggaaagta acagttttgc gttcgatacg
    37441 gcactggttt gggtggcggg attaaacgtg attagccacc tgatcgcgtc gcaatccgcg
    37501 cgtttgctgg acgcctcggc gggcgtagcg gaattggtga acgccgtcag ctatatcgtg
    37561 cttctgggcg cgacattgct cgataacgca catcttttcc gccaagtgcg taccttggcg
    37621 ataagcgaca gcctgaccgg cctcgcaaac tatcgtcacc tggtggacgt cctgcaaggg
    37681 gaactcgaac gctccgggcg aacgaacagg cctttctccg tcctgcttat ggatcttgac
    37741 ggccttaaaa aaattaacga cgtccatggt catgtaatcg gcagccgctc gctctgccgt
    37801 gtggcatcga ttctgcgtct gaattgcaga tccattgata cggcggctcg ttacggggga
    37861 gatgaattcg cgctcatact cccggaaacg accatgatgg ccgcggagca agttgcggat
    37921 cgtgtgcgga cttgcttgat ggcggatcag gaggttccgc gactgaccat aagtattggc
    37981 gtcgcgacgt tcccgaaatg cggacttacc gtccaagaat tgcttgaatt tgcagaccgg
    38041 gctctttatg cacgaaaaga aatatcaaag cggggaaaag tggaaaggcg caggaacgtc
    38101 cccgactcca attagatttt tagcagcaca ggctcgagtc tgtgggattt aaatggtgcg
    38161 cgacgaacca cgtaggttgg ccgcacaccg caaacaattt tacaagcttt gataagttgc
    38221 ggatttcttc caaaaatcag aattgggtaa agaaaaacca acaacaactt cactggtttg
    38281 aaaatctcga atgttgacct tcaagacatt gcatatctgc tctgcagaag tcacattatt
    38341 aatcagcagc aggtcttgcc cttccactac aggaattgaa aatagaaaaa caccgccatt
    38401 cgcgttacca ctaatggcct tggcctggcc ataaaacgcg tcttcatccg gcaatcggca
    38461 ataaacagtc aaatcgatat ccaaatcaat tcgcggactt atccggcgtt ccggtaaatc
    38521 cattgttccg aggtagtctg aagaattcaa tccactctgg tcccgcgatt cgcgcgcttt
    38581 cttgataccg tcgtatatgc ggctcataat tcgtatcctt gggattctga tttgatttga
    38641 attatccaga ctctgacaag caatgttcgc aatcgaaaca gcgtcgaaca ataggagtta
    38701 ggttctagtc aggcctggta cgtggggaca ggtacaatta tcccgcgaat tttactttgg
    38761 gctgttcgat gtaattgcca cgattggttg gctttaaagg gcttggcttg aaccaagcct
    38821 gccgtcgtga gaggattcgg tttcggctcc gcggcacaca gtatgatcgt cgcgtccatc
    38881 gaacctgcgg gaggatact
//
LOCUS       DASZMB010000129        16334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3144640, whole genome shotgun sequence.
ACCESSION   DASZMB010000129 DASZMB010000000
VERSION     DASZMB010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 16334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16334
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3144640"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..118)
                     /locus_tag="VG272_06125"
     tRNA            complement(33..118)
                     /locus_tag="VG272_06125"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(83..85),aa:Leu,seq:caa)
     gene            243..1538
                     /locus_tag="VG272_06130"
     CDS             243..1538
                     /locus_tag="VG272_06130"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF13293.1"
                     /translation="MSSTPKDSLAANSNASVTALAGANTGIPPARWRLGFWSLIFTQF
                     QGAFNDNALKFLVIYLIVERNFPSDIRDRFILLVGALFAIPFILFSLAGGYFADRYSK
                     REVTIATKLFEIAVGVFAIVSLTLGNLPMEAAAVFLISTQGAVFGPSKYGLLPELVPE
                     NKLSWANGVIEMGTFIASITAVMGAGYLADTFRGRQFWSGIILLCLTLAGLFFSYGIS
                     RVPAANPVRRFRANPLGDLCGQLRIIKQDRLLSWAIVGNTYLWFLAALLQFVIVVYGH
                     DVLRVSESQISYLQAAVGIGIGVGSLVAGYLSRGKIETRLVPLGALGMTIFGFLVSRN
                     GLGIWPVRADLCMLGFFGGFYAVPLNALIQHRPELAIRGGVIAAANLFSFVGVFLAAG
                     AYFGLSSLVHFNPREIFLAGAVMTFVATFHAIFLQPNKS"
     gene            complement(1642..2727)
                     /locus_tag="VG272_06135"
     CDS             complement(1642..2727)
                     /locus_tag="VG272_06135"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HWF13294.1"
                     /translation="MAQHKQLTWTDLRVGLFVLAGAILLTVVIFYVTGGSGWGPKYRL
                     RAYLPQVDGLTLGAPVRVDGVDVGNVEKITIAPAKSESPNAKQRNIEVVMRLQKTYQD
                     YVRSDSSAGLVTEGLLGNQYVDIDRGYVGRVLGDNDEIPGRQEKALKEMVERSADLIE
                     SLNSITKQANAVLVDVRNGKGSLGKFLVDEQAYQHLNRSLENVDNMVADIKAGKGTMG
                     KLITDDEMYNHVNSVAGRVDSVLEAVQNQQGTLGKLVYDPALHESIRQFLDNSNGFLA
                     DVRNGKGTLGKLATDDSLFASYKQAGENLASATAKMNSNENTIGKFFADPKFYDNFSG
                     LAGDSRLLIQDFRNNPKKFLHVKLSLF"
     gene            complement(2777..3595)
                     /locus_tag="VG272_06140"
     CDS             complement(2777..3595)
                     /locus_tag="VG272_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWF13295.1"
                     /translation="MSANASGAPVLSFEDVRIGFGTGNVLDGLTFSVASRETKILIGE
                     SGSGKTLILKLAAGLIQADDGTVVMLGHDLSQMSEKELLAFRKGIGFVFQEGALFDSL
                     TVAENVAYCLEEDGVSEEEIESRVRESLRFVEMEDTLDKFPSELSGGMRRRVSIARAL
                     VTRPQVVLYDSPTAGLDPVTAQTIITLILRLRDTQGVTSVLATHRLQDAFGLANYSFD
                     GESGHVVPVAHNGASATLAASTNFVVLRDGKAYFEGSPQKLVETRDSYLRRFLL"
     gene            complement(3714..4541)
                     /locus_tag="VG272_06145"
     CDS             complement(3714..4541)
                     /locus_tag="VG272_06145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF13296.1"
                     /translation="MATTDPNLLEPNDLPVSGVLTEKAKLWVLHVQEYTKLSIRAASD
                     IFTPPYYFSDILEQMDIIGVGSLPIVLLTGFFIGAVMVLQTAAQFERFGQTALTADVV
                     SLALVRELGPSITGLLVTGRNASGMASELGSMVVTEQVDAMRAMGTDPVRKLVTPRLV
                     ATVLMLPLLTAMADFLGLIGGYLVSLFTLRLDPVLFWTRAIDILEFSDLMQGLSKPVV
                     YGFILATVGCYQGLNVKGGTQGVGRATTHAVVAASVFILITDFFLTKLMLYLAGHVF"
     gene            complement(4763..5071)
                     /locus_tag="VG272_06150"
     CDS             complement(4763..5071)
                     /locus_tag="VG272_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13297.1"
                     /translation="MDHPDEIPQILQSHIRTMTETQIVESLRAFAAFRRNEREMLGWL
                     RSVSLMSTEDSTGEIDQLKSDLNLYRRPPVSPFRLWDHLEEALKKRLETLRKRPNNNS
                     "
     gene            5278..6450
                     /gene="aroC"
                     /locus_tag="VG272_06155"
     CDS             5278..6450
                     /gene="aroC"
                     /locus_tag="VG272_06155"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HWF13298.1"
                     /translation="MFRFVTAGESHGQALIAWISGMPAGVPIDFEFVQRELHRRQLGY
                     GRGGRQKIEKDRVEFLSGVRHGKTIGAPIALRVENRDWANWEKSLSVEQLPEGESKER
                     HLTAPRPGHADLAGSMKFNLHDARYILERASARETAARVAAGAIAKLLLRQLDCEVLS
                     HVISVGKARLTRAASWNEIQAICENLESPLRCVDAATEAQMKSEVDVVHRAGNTVGGI
                     FEVVAHNVVTGLGTHVQWDEKLDARLAQAVMSIQAVKGVEIGEGIAAATSYGSEVMDE
                     IGFDAATNRFTRASNRAGGLEGGITNGEDVIVRGYLKPISTLRTPLGTVDMLTKQPVS
                     AAYERSDVCVVPAGGVVGEAMVSIVLTQAMLEKFGGDSLVEMCRNFEGYRQQLKDF"
     gene            6469..6867
                     /locus_tag="VG272_06160"
     CDS             6469..6867
                     /locus_tag="VG272_06160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HWF13299.1"
                     /translation="MNTDSKPIPVSILLDGELTEKILGAAFKVQNKLGAGFLEKVYEN
                     SLSIELRKCGLTVECQKAFPVLYEGAVVGDYLADLVVSEKVVIECKAVTALDVVHEAQ
                     IINYLKASGLRVGLLINFARPKLQFRRLVV"
     gene            6999..7508
                     /gene="def"
                     /locus_tag="VG272_06165"
     CDS             6999..7508
                     /gene="def"
                     /locus_tag="VG272_06165"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWF13300.1"
                     /translation="MIYPITKYGAAVLENPTKAIEKFDDDFAKLCEDMFESMYVAQGV
                     GLAATQIGISKRLAVIDVSVGKNPEAKLVLANPQIIHVEGDQREEEGCLSLPSFRGNV
                     LRPAYVTVRAQNVKGETYEMRGEGLLARAFCHEIDHLDGILFIQHLSMLKRDLIKRRI
                     RKLRKTGDW"
     gene            7526..8482
                     /gene="fmt"
                     /locus_tag="VG272_06170"
     CDS             7526..8482
                     /gene="fmt"
                     /locus_tag="VG272_06170"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HWF13301.1"
                     /translation="MTSLRIVFCGTPEFAVPSLRRLAERPEFSIEAVITQPDRPRGRG
                     GNVTASLVKEAALELGLHVYQPESVKSESSQDFLKRIAPDAVVIIAYGQIIPARLLTI
                     PRLGWLNVHGSLLPKYRGAAPIHWAIANGEAVTGLTTMQINAGMDTGPTLLRREIAIG
                     ADETSPELAARMSAMGAELIVETLLQFDRGELAPSPQDETGVSYAPILKKEDGRINWQ
                     QPAQRIYNRMRGFTPWPGSYSTFRGQICQLWGQPETPGTTEAPSAQKAPGEIIAGAKE
                     IHVACAEGTFLRLESIQVEGRRKITAREFANGARLCDGDKFS"
     gene            complement(8829..9239)
                     /locus_tag="VG272_06175"
     CDS             complement(8829..9239)
                     /locus_tag="VG272_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWF13302.1"
                     /translation="MAIKVLDSWALIAFFENEPAAEQVEILLAKAEAGTHNLLLSAVN
                     WGEIYYNTMREVSQIAAERTVKEISGLAIEIVGVSDTNLDYVRQAAIYKATRRLSYAD
                     AFAAALAKIRNAELVTGDREFKQVEDEIDIAWLR"
     gene            complement(9241..9486)
                     /locus_tag="VG272_06180"
     CDS             complement(9241..9486)
                     /locus_tag="VG272_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HWF13303.1"
                     /translation="MKTTNKTDTIRFTVKGQVVIPIWLRQVFEIEEGTRALVYQEGDV
                     IILKPITPSHIKNLRGSLKGSGVLKSLMEDRKREREL"
     gene            9658..11064
                     /gene="rsmB"
                     /locus_tag="VG272_06185"
     CDS             9658..11064
                     /gene="rsmB"
                     /locus_tag="VG272_06185"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HWF13304.1"
                     /translation="MTISQARTIAFDVLLRVAKQNAYADDVLRGALNESVKTEDAGLA
                     TELALGVLRWQMLLDFLIDRYLKRPADVTDAEVRIALRLGFYQLYFLDRVPAHAAVHE
                     SVELVKRARKRSAVNLVNAILRKAAKEPRDGVSPPDVISSLLSPKIPLAAQLGIQYSH
                     PTWMIERWIRTYGVERARALLQANNCVPALSGYLLDSARSDDTKRLLQFAGCNIQPGR
                     LLRDAWLLEGGNPSACEAVRKGWIVLQDEASQAVARLVAVDPGQTVLDLCAAPGGKTL
                     LLAHAAGPQGRVIAADLHENRVRAMKERFEQSSVRNVETIVLNGAETLPNSKLFERTF
                     DRILVDVPCSGTGTLARHPEIRWKLRAKDISDLHVRQARLLRNALQHLSPGGRLVYST
                     CSLEPEENELVVREVLLAVGSDFQIADARIPIETSLQESVALESVVDAEGFFRTFPGE
                     HGTDGFFGAAIERRKPKS"
     gene            11285..12007
                     /locus_tag="VG272_06190"
     CDS             11285..12007
                     /locus_tag="VG272_06190"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HWF13305.1"
                     /translation="MSFRERIEWVFRMGLLVFILAAAAFLSAITAMRFAVQGREVNMP
                     NLIGKSSGEAQAILQGRGLQLKVVDRVYSDLPINTVARQSPPEGERMKSSQNAHVVLS
                     LGPQNVTTPSLTGESLRVARIQLLQVGLQLGEVTAFTSPAALSDTILQQTPKPGARAA
                     SPRVDLLVAQEAAPPAYVMPWLVGMPLPDADRLLSSSGLKLSKTTYVPSPQWPKGAVT
                     EQTPAQGTKITSESTIEMVVAQ"
     gene            12093..12776
                     /gene="rpe"
                     /locus_tag="VG272_06195"
     CDS             12093..12776
                     /gene="rpe"
                     /locus_tag="VG272_06195"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HWF13306.1"
                     /translation="MNYPVEIAPSILAADFSCLGDGIRAVQEGGAALLHVDVMDGHFV
                     PNISIGVPVVSSLHRATRLPLDVHLMIENPELYIQAFAEAGASMISVHQEATPHLDRT
                     LSMIREHGCKAGAVINPATPVSMLSEVLGKLDYVLVMSVNPGFGGQKFIPGALGKIRE
                     LKRIRESYNYAFRIEVDGGVVPGNVVDLVRAGAEILVAGTSIFHTPDAAEAVRNMKQV
                     ADNALAQKV"
     gene            12798..14045
                     /gene="bamD"
                     /locus_tag="VG272_06200"
     CDS             12798..14045
                     /gene="bamD"
                     /locus_tag="VG272_06200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HWF13307.1"
                     /translation="MKRKNLKQGQTICVIALGAFFLTGCLGKKATAPAAGTSAEPDKV
                     LYEKATDDLKHGRYATGRLALQTVINTYPDSEYLAKAKLAIGDSYFKEGGTAGLKQSI
                     VEYKDFITFFPFLEESAYAQLQVATAHFRQMEKPDRDHAEAVQAEAEYQTFLEKYPNS
                     TLRPQAEQQLRDVQEVLAEGNFRVADFYYLRAAYRAAGARLIELTTRYPLYSQADQAN
                     WMLGQIYEKTEHNDIAAKYYSRIVKDYPLSSLTGDAKNKLVKFGVPVPPADPTAMARM
                     QQEQNAPRPGLVKRSMGILKNNPDFSAAARSGDPTMTPASESGDETIGFSLQPSVPAT
                     TGSSVGSTAAVETITPGSANALPPPATAPADATPAASGSSSGATATDTSATQPATTTA
                     PPANSGQESTSKKKGGLRKLIPF"
     gene            14371..>16334
                     /locus_tag="VG272_06205"
     CDS             14371..>16334
                     /locus_tag="VG272_06205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF13308.1"
                     /translation="MAKILVADDNSNVQKTVALALADLGVEVVAVNNGEAAVKKLPDV
                     MPDLVLADIFMPVRNGYEVCEYVKKDSRFSRIPVVLLVGAFDPLDEREAQRVGADGIL
                     KKPFVPPDPLIAMVKTLLDRTLGDRMVTAAASKSAGSKQSRSGGVALADEHTSASSVS
                     EETREETTQPGLGRVSFGDSDRPVAFSQLLDSPTMELSATPTQLGSVADNDQILTSTR
                     DESLGAPIFWKTENPELELKSGVSDVDADASDEIPAIGWRLGNEALNSAAASNSKAPE
                     EPLELVRDEKDAIPSQVSTIVEPSPLRLQDAASQASLNVEASKAADLAANPLEWMASV
                     PPPKQEEVPENVHEWSAAVPEPTDLIQAKETEPLEPAIDLAAVEIPAAHVPPVPSLEA
                     VPATNSETTLSIPEQAISTVRDTNALSADIPTAANAQSVNDTVRSIPKQDWSDLAASI
                     DPKAMESAQEVIKTSAAHGEHQPAEQAVAIPAPWSSAPTDSKPLASSAEDTAHSMPQH
                     DWADLVSNLKPPSENSNSENAHSSSALVKESLASSEAQSQTTSPASVAPVSKPSNASL
                     ENTDHSLPKLDWADLAASLQTRPSESATPTLAGTSAASPVNGQAVSIPPMTTSPEVPA
                     NQEDNGISVSPADPDPALVEAVVQRVLDKMR"
BASE COUNT         3790 a         4466 c         4305 g         3773 t
ORIGIN      
        1 cgattgaata gaaaaatggt tcaagaaaaa ggtggtgccg gaggagggag tcgaacccac
       61 acggccttgc ggccactgga ttttgagtcc agcgcgtctg ccatttcacc actccggcgc
      121 gtcctagatt ctacacgaca cgcccctgtt gccaaagcct tgctcgatgt gctttccctg
      181 tttacacttc gcagcatgag aaagggttga tctgccagca tcacaagcct ttgagaaaac
      241 ccatgtctag cacaccgaaa gactcattgg ctgcaaactc aaatgcctcg gttaccgcac
      301 tagcgggcgc aaatacgggt atcccgcccg cacgctggcg gctcggcttc tggagcctga
      361 ttttcacgca gtttcaaggt gcgttcaacg acaacgccct gaagtttctc gtcatttatt
      421 taatcgtcga gcgcaacttc ccttctgata tacgtgaccg cttcattctg ctagtgggcg
      481 ctctgttcgc gatccccttc atattatttt ctttagcggg aggctatttt gcggaccgct
      541 acagcaaacg ggaagtcacc attgccacca aactttttga aattgccgta ggcgtgtttg
      601 ctattgtgtc ccttacgctt ggaaatttgc cgatggaagc cgccgccgtt tttctgatca
      661 gcacgcaagg cgccgtattc ggcccttcaa aatatggcct tctccccgaa ctggtcccgg
      721 aaaataagct ctcctgggcc aatggtgtca tcgagatggg cacttttatt gcttccatta
      781 ctgccgtaat gggcgctgga tatttggctg atacttttag aggccggcaa ttttggtccg
      841 gtattatttt actgtgcctc acgctggcgg gccttttttt cagctacgga atttcgcgcg
      901 taccggccgc gaatcccgtg cgccggttcc gcgccaatcc actgggcgat ctgtgcggcc
      961 aactccgcat catcaagcag gatcgcctcc tgagctgggc aattgtgggc aatacctacc
     1021 tctggttttt ggcggcgctg ctgcaatttg tgattgtcgt ttacggccac gatgtattgc
     1081 gcgttagcga atcgcagata agctatttgc aagctgcagt cggcattggc atcggagtcg
     1141 gaagcctcgt tgcgggctat ctttcgcgag gaaaaatcga aacgcgatta gttcccctcg
     1201 gtgcattggg aatgacgata ttcggtttcc tcgtttcgcg gaatgggctt ggcatctggc
     1261 ccgtgcgcgc cgacctttgt atgctcggtt ttttcggagg tttttatgcg gtccccctga
     1321 acgccctcat ccaacaccgc cctgaactcg caataagagg tggcgtaata gccgccgcaa
     1381 atttattttc atttgtaggc gtgttccttg ccgcaggcgc ttattttggc ctttcttcac
     1441 tggttcattt taatccgcgg gaaatttttc tggcgggagc ggtgatgacc ttcgtagcga
     1501 cattccatgc aattttcctg cagcccaaca aatcataaaa tagatctaag tggcgcgggc
     1561 ttcggcctgc ggaatttagt ccttgcaagg cccacccccc acagactgaa gtctatgcta
     1621 catacccccg ctatttggaa cctagaaaag cgacaatttc acgtgcagga attttttggg
     1681 attgttccga aaatcttgga tcaacaatct cgaatcgccc gcgagccctg aaaaattgtc
     1741 gtagaatttc ggatcggcga aaaatttccc aatcgtattt tcgttggaat tcattttcgc
     1801 ggtcgcgctg gccagatttt cgcccgcctg tttgtaactc gcaaacaagg aatcgtccgt
     1861 ggccagtttg ccgagagttc ccttcccgtt cctcacatcg gcaagaaacc cattgctgtt
     1921 atccaaaaac tgcctgatgc tttcgtggag ggctggatcg tagaccagct tgcccaaggt
     1981 cccctgctgg ttctgtacgg cttccaacac gctatccacg cggcctgcaa cggaattcac
     2041 gtgattatac atttcgtcgt cggtgatcag tttacccatc gtgcccttgc cggccttgat
     2101 gtccgccacc atgttgtcca cgttttcaag ggaacgattc aggtgttggt aagcctgctc
     2161 atccactaaa aacttgccca gcgatccttt gccattgcgc acgtccacca atacggcatt
     2221 tgcctgcttg gtgattgagt tcaggctctc gatcaggtcc gcgcttcgct cgaccatttc
     2281 tttcaatgct ttttcctgcc gccccggaat ctcatcgtta tctcccagca cgcgtcccac
     2341 gtagccacga tcgatatcca catattggtt gcccagcaac ccctcggtaa cgagcccggc
     2401 ggaagaatcc gagcggacat agtcttgata tgttttttga agtcgcataa ccacttcgat
     2461 gttccgttgt ttggcgttcg gactctcaga ttttgccggg gcaattgtta tcttttccac
     2521 gttgccaaca tcgactccat cgacgcgcac aggcgcaccc agagtcaatc catccacctg
     2581 aggaagatac gcgcgcaatc tatacttggg cccccagccg ctgcctccag tcacataaaa
     2641 tatgaccact gtaagtagta tggctccggc cagaacaaac aatcccactc gaagatcggt
     2701 ccacgtcaat tgtttgtgtt gtgccatcgg ttctcctccg agcaacatct aacggatgta
     2761 gatgccattt taactgctac aataaaaatc gtctcaaata cgaatcgcgc gtttccacca
     2821 atttctgcgg actcccctcg aaatacgctt ttccatcgcg caacacgacg aaattagtgg
     2881 atgccgccaa agtcgctgag gccccgttat gtgccacggg aacgacatgg ccggattccc
     2941 cgtcaaaact gtaattcgcc agcccaaatg cgtcctggag ccgatgtgtg gcgagcacgg
     3001 acgtaacacc ttgcgtatcg cgtaaacgca aaattaatgt gatgatcgtt tgtgcggtaa
     3061 ccgggtcaag ccctgctgtc ggagaatcgt acaggactac ctgaggccgc gtaacgaggg
     3121 ctcgcgcgat cgacacacgc ctcctcatcc cgcccgatag ctcggatgga aatttatcca
     3181 gcgtgtcttc catttcgaca aatcgcagag attcgcggac gcgagactca atttcttctt
     3241 ccgacacgcc gtcttcttcc aggcaataag ccacattttc ggccactgta agtgaatcga
     3301 atagagcgcc ttcctgaaat acaaatccga taccttttct aaaggcgagt aattcttttt
     3361 cagacatttg ggagagatcg tgaccgagca ttaccacggt tccatcatca gcctggatca
     3421 aaccggctgc caacttaagg atcaacgttt ttccagaacc gctttcccca atcaaaattt
     3481 tagtttcacg cgaggctacg gaaaaggtga gtccgtccag aacattgcct gtaccgaaac
     3541 caatgcggac atcttcgaag gaaagaacgg gggcaccgga tgcgttcgcg ctcattctga
     3601 cagtcgcgct catcctaaaa tgatgcgtcc acgccaggat gggatcgttg gcacgttcat
     3661 cttatggatt atagatggaa acctgcccgg tgagggaacg attgcaagtt tgcttaaaaa
     3721 acgtgtccag caagatatag catcaatttt gtcaggaaaa aatccgtaat caggatgaac
     3781 acggacgcgg caaccaccgc atgcgtagtc gcgcgtccta cgccctgcgt tccccctttc
     3841 acgtttaatc cctgataaca accaaccgtt gcaaggatga agccgtagac gactggtttg
     3901 ctgagcccct gcatcagatc ggaaaattcc agaatgtcga tcgcgcgagt ccaaaacaag
     3961 actggatcca ggcgaagcgt aaacagcgaa acgagatagc cgccgatcag gccaagaaaa
     4021 tcggccatcg ctgtcagcaa tggcaacatc aaaacggtcg ccaccaaacg cggcgtaacc
     4081 agttttcgaa ccggatccgt gcccatggcg cgcatcgcgt ccacttgctc ggtcaccacc
     4141 atcgatccca attcggaagc catcccggaa gcgttgcgcc cagtaaccag cagtcctgtg
     4201 atcgacggcc ctaattcgcg gaccaaggcc agcgaaacca cgtccgctgt aagcgccgtc
     4261 tgcccaaatc tttcaaattg cgcagctgtt tgcagcacca tgaccgcgcc aataaaaaaa
     4321 ccggtgagca aaacaatcgg cagcgaaccc acgccgataa tgtccatttg ttccaaaatg
     4381 tcagaaaaat agtaaggggg tgtaaatatg tctgaagctg cgcggatcga caacttcgtg
     4441 tattcctgca catgcaggac ccacagcttc gctttttccg ttaatacgcc cgatacggga
     4501 aggtcgtttg gctcaagaag atttgggtca gtggtcgcca tcagtcgccg ttggctgttc
     4561 cttgagcgca gttgggttcg aaaacagcct actggtcatt ggaaaggacg tcaagcatcg
     4621 tgagtacaag cgattaccaa ttgtaagaca ttcaatgctc ccgccgtgtc aacgcttagg
     4681 tactgcctta attaaatatt attcgaattc aggagggccg agctcggcta ttcgaactgc
     4741 atatcgtcgc gggcttcagc tgctaagagt tgttattagg ccttttcctg agcgtttcga
     4801 gccgcttttt cagcgcttcc tcgagatgat cccagaggcg gaaggggctg acgggaggtc
     4861 gccgataaag attcaaatct gatttgagct gatctatctc acccgtggaa tcttctgttg
     4921 acatgagaga aacagatctg agccagccca gcatctcgcg ttcattgcgt cgaaaagccg
     4981 cgaatgcccg aaggctttca acgatctgag tttcggtcat cgtccgaatg tgggattgca
     5041 aaatctgggg aatttcgtct ggatgatcca tacagagaat cttaccagcc ttggtcagtt
     5101 ctacggccga caacgagatc agctacatgc accccgcagc cacagggaca gagcaagatt
     5161 tggttcctca tagatacagc ttaggttggg gcggcataga aaagcaaaat gagtcactac
     5221 cctctcttat gatcgcttta cgcgccgtgc agggtccgat agaatgtcgt gccttccatg
     5281 tttcgctttg tcacagcagg tgagtctcac ggtcaggcgc tgattgcgtg gatcagcggc
     5341 atgccagcgg gtgtgcccat cgactttgaa ttcgtgcagc gcgagctgca ccgccgccaa
     5401 ttaggctacg gccggggtgg ccgccagaaa attgagaagg accgcgttga atttctttcg
     5461 ggcgtacggc atgggaaaac cattggtgcg ccgatcgctc tacgcgttga aaatcgcgat
     5521 tgggccaact gggaaaagtc cctctccgtc gaacaactgc cggaaggtga gagcaaagag
     5581 cgccacttga ccgcgccgag gccgggccat gcggatctcg ccggctccat gaaatttaat
     5641 ttgcacgatg cgcgatacat tttagagcga gcatccgcgc gggaaaccgc agcgcgcgtc
     5701 gcggccggcg cgattgcgaa gttgcttctg cgccaactcg attgcgaagt gctgagccat
     5761 gtcatttcag ttggcaaggc gcgcttgacg cgcgccgcct cctggaatga aattcaagcg
     5821 atctgcgaaa atctggaatc gcccttgcgc tgcgtcgatg ccgcgaccga agcgcagatg
     5881 aaaagtgaag tcgacgtcgt gcaccgtgcc ggaaatacgg taggcggaat ttttgaagta
     5941 gtggcgcata acgttgtcac cgggcttggc acgcacgtgc aatgggacga aaagctcgat
     6001 gcgcgcctcg cgcaagccgt gatgtccatc caggcggtaa aaggcgtgga aattggcgaa
     6061 ggaatcgcgg ccgccacctc ctacggctca gaagtgatgg acgaaattgg attcgacgcc
     6121 gccaccaatc gtttcacgcg tgcatccaac cgcgccggtg gcctggaagg cggcatcacc
     6181 aatggcgaag acgtaatcgt ccgtggatac ctgaaaccaa tttccacgct gcgcacgccg
     6241 ctcggcaccg tagatatgct cacgaagcaa cccgtttccg ccgcatacga acgctcagac
     6301 gtctgcgtag ttcccgctgg cggagtggta ggcgaagcca tggtttccat cgtcctgacg
     6361 caagccatgc tggaaaaatt tggaggcgac tcgctggtag agatgtgccg caattttgag
     6421 ggatacagac aacaactaaa agacttttga aaccacggat gcacacggat gaacacggat
     6481 tcaaaaccga tacctgtttc aatcttgctg gatggtgaac tcactgagaa aattctcggt
     6541 gccgccttta aggtgcagaa caaattgggt gcaggcttcc tggaaaaagt ttacgagaat
     6601 tccttgtcaa tagagcttag gaagtgcggc cttactgtag agtgccaaaa agcttttccc
     6661 gttctatacg aaggtgcagt tgtcggggat tatttagccg atttagtagt gtctgaaaag
     6721 gttgttattg aatgcaaagc cgttacagct ttagacgtag ttcacgaggc gcaaataata
     6781 aattatttaa aagcaagtgg gcttagggtg gggcttttaa ttaattttgc gcgccccaaa
     6841 cttcaatttc gaagattggt tgtttgaatg acgctgaaag atcgaaagtt gagcggcgtg
     6901 gggaataaat atcatttaag atatttggat ttatcagctt ttatctgtgt tcatccgtgt
     6961 gcatccgtgg ttcccaacga atcgagtgag cagctgagat gatctatccg attacaaagt
     7021 atggcgcggc ggtgctggaa aatccgacca aggcgattga aaaatttgat gacgacttcg
     7081 caaagctttg cgaggatatg ttcgagtcga tgtatgtggc gcagggcgtc ggcttggcgg
     7141 caacgcaaat tgggatatcg aagcgcctcg ctgtgattga cgtaagcgtt ggcaagaacc
     7201 ccgaggcgaa actcgtgctt gcgaatcccc agattattca cgtcgaggga gatcagcgcg
     7261 aagaagaagg ctgcctctcg cttccgagtt tccgcggaaa tgttttgcgc cccgcctatg
     7321 tgaccgtgcg cgcgcaaaat gtaaaaggag aaacttacga aatgcgtggc gagggattgc
     7381 tggcccgcgc gttttgccat gagatcgacc acctcgatgg aatccttttt attcaacatc
     7441 tcagcatgct gaagagagat ttaataaaac gccggatccg taaattgcgg aaaaccggcg
     7501 actggtagat tttttcatcg ctgccatgac ttccttacgc attgtttttt gcggtacgcc
     7561 ggaatttgcg gttccctctc tccggcggct tgcggagcgt cccgaatttt ccatcgaagc
     7621 ggtcatcact cagccggacc gcccgcgcgg tcgcggcgga aatgtcacgg cgtcactcgt
     7681 aaaagaagcg gcgctcgaac tggggctgca cgtctaccaa ccggaatccg tcaagtccga
     7741 atcttctcag gattttttaa agcggatcgc cccggatgct gtcgtgatta tcgcctacgg
     7801 ccaaattatt ccagccaggc tcctgacaat tccccgcctc ggctggctta atgtgcacgg
     7861 ctctctgctg ccgaagtatc gcggtgcagc gcccattcat tgggccatcg caaacggtga
     7921 agcagtgact ggcctcacca ccatgcaaat taacgcgggg atggataccg gcccaactct
     7981 tttgcgtcgt gagatcgcga ttggcgcgga cgaaacttcg cctgagttag ccgcgcgcat
     8041 gagcgcgatg ggcgcagaac tgattgtaga gacgctactg caattcgatc gcggagaact
     8101 cgcgccgtcg ccgcaggatg aaaccggagt ttcctacgcg cccatcctga aaaaagaaga
     8161 tggccgaatc aattggcagc agccggcgca gcgaatttac aaccgcatgc gcggcttcac
     8221 cccgtggccc ggctcatact caactttccg cggccaaata tgccagctgt ggggtcagcc
     8281 agaaacgccc ggaacaactg aggcgccaag tgcgcaaaaa gcgccagggg aaattatcgc
     8341 aggcgcaaag gaaatccatg tagcatgtgc ggaagggaca ttccttcgat tagagtccat
     8401 acaagtggaa ggccgcagaa aaattaccgc gcgcgaattt gccaacggcg cacggctatg
     8461 tgacggagac aaattttctt agaaattact tgataactag aatccagctg ctctgttgac
     8521 atccccggtt ccgctgccga tctgctgtag cctgcctctt cagaggcggg ttttttgacg
     8581 ctccagcatc taaaccctcg cctctgaaga ggcgagctac aattcaggct ccacaaaacg
     8641 ggcgaaactt tctgaagcat ccaggatatc aaccgagccc agccatctca tatagatttg
     8701 tgggtcgcgg cttcagccgc gacgttcaga ggcgtgaaaa tcgaaggctt tagcttctga
     8761 agtcctacct ggtacctggg cgagccattt acgagatggt ttccagcacg aatgcaatgc
     8821 cgtaaaacct accgcagcca tgcaatatcg atttcatctt cgacctgttt aaattctcga
     8881 tcccccgtca caagttccgc atttctaatc ttagcgagcg ctgcagcaaa tgcatctgca
     8941 taggataatc ttctcgttgc tttataaata gcggcctgcc gcacataatc cagattcgta
     9001 tcactgacgc ccacgatctc aatcgctaag ccggaaattt cctttacagt gcgctccgcc
     9061 gctatctgcg aaacttcacg cattgtgttg tagtagattt cgccccagtt gacggcggag
     9121 agtagcaggt tgtgcgtgcc agcctcggct ttcgcaagga gaatttcaac ctgctccgcg
     9181 gccggctcat tttcaaaaaa tgcgatcaaa gcccagctgt ccaacacttt gattgccata
     9241 ctatagttcc cgctcgcgct ttcgatcctc catcaacgat ttcagaactc ccgaaccctt
     9301 gagcgagccg cgcaaatttt taatgtgact gggcgtaatt ggcttcagga taatcacgtc
     9361 cccctcttga tagacgagcg cgcgcgtccc ttcctcaatt tcaaacacct gccgaagcca
     9421 gatggggata accacctgac ctttgacggt aaatcggatg gtatcggtct tatttgtagt
     9481 cttcatgatt aatcatccta ccacggttaa ataagtaagt catcatatcg attgtaagac
     9541 ataattgttc gagttatact tacacgaagt acgcttacca tacaatcgca ttttattgca
     9601 cctcattaaa tcaaaaacat atattcaccg ccgttgtatg cttccaagcg cacattcatg
     9661 accatttcac aagccagaac gattgcattc gatgtattgc tgcgcgtcgc gaaacaaaac
     9721 gcctatgcgg acgatgtgct gcgcggcgcg cttaatgaat ccgtgaagac cgaggatgcg
     9781 ggcctcgcca cggagcttgc acttggcgtc ctgcgctggc agatgctcct cgatttttta
     9841 atcgatcggt atttgaaaag accggcggac gtgacggatg cggaggtccg catcgcgctg
     9901 cgcctgggtt tttatcaact ttactttctc gaccgcgtgc ccgctcacgc cgctgtccac
     9961 gagtctgtgg aattggtgaa gcgcgcaagg aaacgctccg cggtcaatct ggtgaatgcc
    10021 atcctccgca aagccgcgaa ggagccgcgt gacggcgttt cgccacctga cgttatttcg
    10081 tctctcctgt ctccgaagat tccgcttgcc gcccagctgg gaattcaata ttcgcaccct
    10141 acatggatga ttgagcgctg gattcgaacg tacggagtgg agcgcgcccg cgctctgcta
    10201 caggcgaaca attgtgttcc ggcgctatca ggttatctgc tcgattcagc aagaagcgat
    10261 gacaccaaac ggttgcttca attcgctggc tgtaacatcc agcccgggcg gctgctgcgc
    10321 gacgcatggc ttttggaggg aggcaaccca tccgcgtgcg aagctgtccg caaaggatgg
    10381 atcgtgctgc aggatgaggc ttcacaggcc gtagcgcgtc ttgtggcggt cgatcccggg
    10441 caaactgtgc tggacctgtg cgccgcgccg ggcggcaaga cgctgcttct cgcgcacgcg
    10501 gccgggcctc agggtcgtgt gattgcggcg gacctgcacg agaatcgtgt ccgcgccatg
    10561 aaagagcgat tcgaacaatc cagcgttcga aatgtggaga cgattgtatt aaacggcgcg
    10621 gaaactctgc caaattcaaa actattcgaa cgcacatttg atcgcatact ggtcgatgtt
    10681 ccctgctccg gcacgggcac gcttgcacgg cacccggaaa ttcgatggaa gcttcgagcg
    10741 aaagacatca gcgatttgca tgtgaggcag gcgcggctgc ttcgaaatgc gttgcagcat
    10801 ttatctccgg gagggcggct ggtgtattcc acgtgctcgc ttgaaccgga ggagaatgaa
    10861 ctggttgtgc gtgaagtgct tctcgcggtt ggcagcgatt ttcaaatcgc ggatgctcga
    10921 attcccatcg aaactagctt gcaggaatcc gtcgcgctcg aaagcgttgt cgatgccgag
    10981 gggttctttc gcacttttcc tggtgagcac ggaactgacg gcttctttgg ggcggccatc
    11041 gaacggcgaa aacccaaaag ctaaatgctt aacacaaagg acacagagaa aagacataga
    11101 gggtcacaga gaaggctggg aacagtttag gtttagcgac acagtttgtg atttgattcc
    11161 ctttttctgt tccatcattt tttctctgtg gttctctggc gtttctctat gtcctctgtg
    11221 ttaaatcttt ttcgcgcctc tggcacgctt ccccctcgca tgctaaattg atcccggtga
    11281 acgaatgagc tttcgcgagc ggattgaatg ggtatttcgc atgggcctgc tggttttcat
    11341 cctggcagcg gcggcgtttt tgagcgccat tacggccatg cgtttcgcgg tgcaaggccg
    11401 cgaagtgaac atgccgaatc tcatcggaaa aagttctggg gaagcgcaag cgattctgca
    11461 aggccgcggc ctgcaattaa aagtggtcga tcgtgtctat agcgatttgc cgatcaacac
    11521 ggttgcgcgg caaagtcctc cggaaggcga gcgcatgaag agttcgcaga atgcgcacgt
    11581 cgtgctcagc ctcggcccgc aaaacgtaac cacaccatcg ctgacgggag aatctctccg
    11641 cgtcgcacgt attcagttgc tgcaagtagg tttgcaactc ggcgaggtca ctgccttcac
    11701 gtctcccgcg gcgttatccg atacgatcct gcagcaaacg ccgaagccag gcgcacgcgc
    11761 cgcaagccct cgcgtcgact tgctcgttgc gcaggaggct gcgccgcccg cttacgtcat
    11821 gccatggctc gtaggcatgc ccttgccgga cgcggacagg ttgcttagtt cctctggcct
    11881 gaaactttcc aagaccacat acgtcccatc cccgcagtgg ccgaaaggcg ctgtgaccga
    11941 gcagactcca gcccaaggaa cgaaaattac cagcgaatcg acaattgaaa tggtcgtagc
    12001 gcagtagagc ccgcagcgga agctggatat ttttttcgaa tcgtgcagtg caggcaaaag
    12061 cgcgaatctg gtaagatgtc cgcggagaaa ccgtgaacta tcccgttgaa atcgctccat
    12121 ccatacttgc tgcagatttt tcctgcctgg gagacggaat tcgcgcggtc caagagggcg
    12181 gcgcggcgct tctgcatgtg gatgtgatgg acggacattt cgtccccaat atttcgatcg
    12241 gagttcccgt cgtttcctcc ctgcataggg caacccggct gcccctcgac gtgcatctga
    12301 tgattgagaa tccggagctg tacattcagg cgtttgcgga ggcgggcgcc agcatgattt
    12361 ccgtgcacca ggaagccact ccacacctgg accggacgct cagcatgatt cgtgaacacg
    12421 ggtgcaaggc gggcgcggtg attaatcccg cgacaccggt ttccatgctt tccgaagttt
    12481 tgggtaagct ggattacgta ttagtgatgt ccgtcaatcc ggggttcggg ggacaaaaat
    12541 ttattcccgg ggctcttgga aagattcgcg agctaaaacg aattcgagag agctataatt
    12601 acgcgttccg gatcgaagtg gatgggggag tcgttccggg caatgttgtc gatttggttc
    12661 gagccggagc cgaaattctg gttgccggaa cctccatatt tcatactccc gatgccgccg
    12721 aagcggttcg caacatgaaa caggtggcag acaatgcgtt ggcacaaaaa gtttagggac
    12781 gaagttcagg gtaattgatg aaaagaaaaa atttgaaaca aggccagacc atttgcgtca
    12841 tagctctcgg cgcgtttttc cttaccgggt gcctcggcaa aaaggcaacc gctcccgcag
    12901 ccggcacttc cgcggagccg gacaaagttc tttacgagaa ggccaccgat gacttgaaac
    12961 acggccgcta tgcgaccggt cgcctggcct tgcagaccgt gattaacacg tatccggaca
    13021 gcgagtatct ggccaaggcg aaactggcta tcggcgattc gtactttaaa gaaggcggaa
    13081 cggcgggcct gaaacaatcc atcgtcgaat acaaagattt tattactttc ttccctttcc
    13141 tcgaggaatc ggcatacgcc cagttgcagg ttgccaccgc gcattttcgc cagatggaaa
    13201 agccggaccg cgaccatgcg gaagccgtgc aggcagaagc ggaataccag actttccttg
    13261 agaaatatcc aaatagcact ttgcgccctc aggcggaaca gcaattgcgc gatgtgcagg
    13321 aagttttagc ggaaggaaat ttcagggtag ccgattttta ttatctgcgc gccgcatacc
    13381 gtgccgcggg cgctcggttg atcgagttga ccacacgcta cccgctctac agccaggccg
    13441 atcaggccaa ttggatgctc ggccagattt atgagaagac cgaacacaac gacatcgccg
    13501 cgaaatatta cagccggatt gtgaaagatt acccgctctc ttccctcacg ggcgatgcta
    13561 aaaataaact ggtgaagttt ggcgttcctg ttccgccagc ggatccaacg gcaatggcgc
    13621 gaatgcagca agagcaaaac gccccgcgcc cgggactcgt gaagcgctcg atgggcattc
    13681 tgaaaaacaa ccctgatttt tcagccgccg cgcgcagtgg cgacccgacc atgacgcccg
    13741 caagcgaatc gggcgatgaa accatcggct ttagcctgca gcctagtgtg ccggccacta
    13801 cgggttccag cgtcggaagc acggcggcgg tggaaaccat tacaccggga agcgcgaacg
    13861 cgctgccccc gccagcgacg gctcctgcgg atgcgactcc tgctgcatcg ggctcttcct
    13921 ccggagcaac agcaactgat acgagcgcca cgcaaccggc aacgacgact gcgccaccag
    13981 caaattccgg ccaggagtcc accagtaaga aaaagggtgg cctgcgcaaa ctgattccct
    14041 tttaattaga atccatccgg atctattgac atctctggtt ccgctgccga tttgtaggcc
    14101 tccctcctca gaggcgggat ttttggcgct ccagcatcta aaccctcgcc tctaaagagg
    14161 cgagctacaa ttccggcgcc acaaattctc atactgaaaa tttcctacct gtcccctcgt
    14221 acaggctgtt tgcaggtcta gccgattccg acacacgagt tgtgtcgctg tattgactca
    14281 cccgaatccc gatgttacga atggtttagg gcattatctg ccttttcgcg ttatagcttt
    14341 ttgtatttgc ggtaatcggg aggagtgcac gtggcaaaga tcctggtagc cgacgataac
    14401 agcaacgtcc agaagacggt cgcgcttgct ttggccgatc tgggcgtgga agtagttgcg
    14461 gtcaacaacg gcgaagccgc ggtaaaaaaa ctccccgatg tgatgcccga tctcgttctg
    14521 gcggatattt ttatgcccgt tcgcaatgga tatgaggttt gcgaatacgt aaaaaaagat
    14581 tcgcgtttct cccgaattcc cgttgtgctg ctggtgggcg cgttcgatcc tctcgatgag
    14641 cgggaagcgc agcgcgtggg cgcggacgga attttaaaaa agccgttcgt tcccccggac
    14701 ccattgattg caatggtaaa aacacttctg gatcgcacgc ttggcgatcg gatggtaacc
    14761 gctgcggcct cgaagtccgc aggttccaag cagagcagat ccggtggcgt agcgcttgcc
    14821 gatgagcata cttctgcgtc ttccgtttcc gaagaaacac gggaagaaac aacacaaccc
    14881 ggacttggcc gcgtctcttt cggcgatagc gaccgccctg ttgcgttttc ccagctttta
    14941 gattccccca cgatggagct ttccgccacc ccaacacaat tgggaagtgt ggcggataat
    15001 gaccagattc tgaccagtac gcgagacgaa tctcttggcg cgcccatttt ctggaagacg
    15061 gaaaatcctg aactggaatt aaaatccggc gtttccgatg ttgatgcgga tgcatcggac
    15121 gaaattccag cgattggttg gaggctcggg aatgaagccc tgaactcggc cgctgcatcg
    15181 aattccaagg cgcctgagga accgttggag ctggtgcgcg atgagaagga tgcaattccc
    15241 agtcaggtgt caactattgt tgagccctct cctttgcgct tgcaggatgc ggcttcgcag
    15301 gcttccctga atgtggaagc gtccaaggct gcagacctcg ccgccaatcc gctcgaatgg
    15361 atggcatctg tccctccacc aaaacaggaa gaagtcccgg agaacgtgca tgagtggagc
    15421 gcggccgtcc cggagccaac ggacctgatt caggcaaaag agactgagcc cctggaaccc
    15481 gcaatagact tagccgccgt ggaaattccg gctgcccatg tgccgccggt cccttcgctt
    15541 gaggccgttc cagcgacgaa ttcggaaaca acgctttcaa ttccagaaca agccatctca
    15601 acggttcgag acacaaatgc tttgagtgcg gacataccta cggcggcaaa cgcccagagc
    15661 gtcaatgata ccgtgcgatc gattcccaag caggattggt ccgacctggc ggctagcatc
    15721 gacccaaagg caatggaatc tgctcaggaa gtgataaaga cttcagcagc tcatggcgaa
    15781 catcaaccag ccgagcaagc agttgcgata cctgctcctt ggtcttccgc gccgaccgat
    15841 tcgaagcctc tggcatcgag cgccgaagac acagcccact cgatgcctca gcacgattgg
    15901 gccgatctcg tgtcgaatct gaagccccca tcggaaaatt cgaattcgga gaatgcacat
    15961 tcctcttccg cgttagtgaa agagtcattg gcttcctccg aagcccagtc acagacaact
    16021 tctccggcat ctgtagcgcc cgtttcgaag ccatctaacg ccagtctgga aaatactgac
    16081 cattctttgc cgaagctgga ttgggccgat ctggctgcca gtcttcaaac gcggccttca
    16141 gaatccgcga cgccaactct tgccggcact tctgccgcgt cacccgtgaa cgggcaagct
    16201 gtatccattc cgccaatgac gacctcgccg gaagttccgg caaatcaaga ggacaatggg
    16261 ataagcgttt cgccggcaga tcccgatccc gcgctagttg aagccgttgt ccagcgcgtc
    16321 ttggacaaga tgcg
//
LOCUS       DASZMB010000130         5902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4795139, whole genome shotgun sequence.
ACCESSION   DASZMB010000130 DASZMB010000000
VERSION     DASZMB010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4795139"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            377..1177
                     /locus_tag="VG272_06210"
     CDS             377..1177
                     /locus_tag="VG272_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13309.1"
                     /translation="MAAVASDVKGAAHMKFFDIALAVCIFCIPVAAQERKDGAPQPAT
                     SVLVNGQPIRGKVLEIDGKHFVAVEDLAQSLRGTIAYGEGQLLLTFSKLPGPGPSPSS
                     QSSSAMASASIAPVPTITARPLETGGVKGTLTYFFDFHTGNRPDAGAKIWLVQGRAEI
                     PADQNFVAGNTSVGTAANPEQYRAVKYSSADEKGNFEMLDVPAGQYTLILQSAHTKWT
                     LNDKRDIFGRGNGHNARDSSGRVIFLSVTVKQGEVAEASKDFGPGIDK"
     gene            complement(1445..2455)
                     /locus_tag="VG272_06215"
     CDS             complement(1445..2455)
                     /locus_tag="VG272_06215"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF13310.1"
                     /translation="MPNDESKYVRPVDVHAHFFPQAYLDVLAEYGPKFGVEYRAAEEG
                     FYIKTEVRFQGPLPYRSVDLKLRIADMDAQGTAMQAMSLTGPMAHWGDEEVSHKLASA
                     YNDAASAAHVQYPTRLFGLLTLPMMFPDRAIDELNRASKLPGLRGVYMGTNINNRDLD
                     DPLFEPILARIEALDLPIFLHPLQVAGGPRIAPFSLVNLIGFPLDTTIAVCHLIFGGV
                     LDRHPNLRFVLPHGGGVLLMLLGRMDHGSIVRPEIQKLRLPRPPSKYLDRFMYDTIVH
                     SKSVMEFVISEVGADRIMVGSDYCLDMGYDQPLQVLDQVNMTPAQKRMILGENAARLL
                     KL"
     gene            3227..3721
                     /locus_tag="VG272_06220"
     CDS             3227..3721
                     /locus_tag="VG272_06220"
                     /inference="COORDINATES: protein motif:HMM:NF017758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HWF13311.1"
                     /translation="MKSLRELFDLELNYAYDCEQKLVEKGLPAMIEAATSPQLQAALK
                     QHLEETRLHVRRLEQVFGAIGTEPDTKDNEVLDELMNAAEDSVSNIEQSALRDAALIV
                     NGNMVEHLEIATYGTLAAFARNLGFQNVVSVLEQTLQEEKTADAKLTQLAETMNQQAK
                     RQSA"
     gene            3785..4174
                     /locus_tag="VG272_06225"
     CDS             3785..4174
                     /locus_tag="VG272_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13312.1"
                     /translation="MPTKRKTKQLRKKKPAPKSRARRSSARDPIRSGTLRSALDPTRT
                     REFQESGPETEAPETGASAGDLEGLETDDNEDFESVAELVSEGQDLEAEELESIEKAP
                     NADQGDLKPRKVPSGTKPGKFSDRNRL"
     gene            4427..4861
                     /locus_tag="VG272_06230"
     CDS             4427..4861
                     /locus_tag="VG272_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWF13313.1"
                     /translation="MKPVYGTAILATFLISLVMGLHAQPQAKYSAEQVKRGKYLVEEV
                     AKCPECHTPRDKNNQLDPQRWLQGAPIWIQPVFKTPGWADEAPSLAGLTSYTDDQATR
                     VLEKGERANGGGTVQPPMHIYRMHHEDAQAIIAYLRSVPNHP"
     gene            complement(4982..5779)
                     /locus_tag="VG272_06235"
     CDS             complement(4982..5779)
                     /locus_tag="VG272_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWF13314.1"
                     /translation="MTESNAPQLGHFHVVARGKGHALEFSPMRAPYGLEIIESCLTCP
                     HREDRLFCNLSPGAVQRLASITSPSSYPKGATLFVEGQAGRGVFILCAGRVKLYTSSI
                     DGKTLIARLSEPGEVLGLPSTVTGMPYELTAEVVEPTQANFVSANDFLNFLREYGEVA
                     LRVAQQLGQTYHTALAEMRTIGLSHSAEEKMARFVLDLTASQKEEKGAVKAKLTLTHE
                     EIAQMIGTSRETVTRVLSIFRKKNLVVIKGSTLTLKDRPGLQRLVED"
BASE COUNT         1441 a         1658 c         1564 g         1239 t
ORIGIN      
        1 ccccgtcacg ccgcccatct tccacttgtt cggcaaaacg tgcggccagg tcaggtccgc
       61 tttaaatttg ggaaccgcag ccgtctgcgc tgcaggtccc ttttcaaatg caaacgcgca
      121 cacgaatcca agcgcgagca cgcctgcaca aacccatttg caccttgcca tcggaaattc
      181 ctcctcgatc aaatgtattc ctcaccccag gagttcagtg cgcgcagtct atgacgcgaa
      241 ggcaggaagg tcaacctgcg atgtaagggt gaatcggtgg cccgggaagt gacaaggctc
      301 ccacgtatcg agccccgcga tggtctgcta ttattcgaaa catgttcgca atccgattga
      361 tagtgaagat gcagtcgtgg cagcggtagc gtcggacgtg aagggagcgg cccacatgaa
      421 atttttcgat attgctctcg cggtatgcat tttctgcatt ccagtcgcgg cacaggaaag
      481 aaaggacggc gccccccagc ccgcaacgtc ggttctcgtc aacggtcaac ccattcgagg
      541 taaggtgctg gagatcgatg gaaaacattt cgtggctgtg gaagatctgg cccaaagcct
      601 ccgcggaacg atcgcgtatg gcgagggcca acttttactc acattttcaa agctgccggg
      661 tcccgggcct tccccttcgt cccaatcttc ttccgcaatg gcgtccgcat cgatagctcc
      721 tgtgccgacc atcacggcac ggccactgga aacaggtggc gtaaaaggaa cgctcaccta
      781 ttttttcgat tttcacacgg gaaatagacc ggatgctgga gcgaaaatat ggctcgtgca
      841 agggcgtgcg gaaattccag cggatcaaaa ttttgttgct ggcaatacat ccgtcgggac
      901 ggccgcgaac ccggagcaat acagggctgt taaatattcc agcgcggacg aaaaaggaaa
      961 ttttgagatg cttgacgttc cggcaggcca atacacactg atcctgcagt cagcgcacac
     1021 caagtggacg ctgaacgata aaagggatat cttcggacga ggcaacggac acaacgcgag
     1081 agacagcagc ggacgagtaa tatttctaag tgtgaccgtt aaacaaggtg aggtcgccga
     1141 ggcctcgaaa gactttggtc ctggtatcga taagtgaagt gctcgtgtcg cggtcagcgt
     1201 ggcatcagcg cggatgagac tcagcttcgc gcaaaatttg caagataatc cggcccctca
     1261 acaaactgca aatgtccgcc gtcgcttcga ctccgcgggt ctagcaggtt gcggaaaaag
     1321 tgctttttct gtcattccga gcgaagcgag gaatctctct tcgagttaaa cgtaagagag
     1381 taaacgaagg aaagattcct cgcttcgctc ggaatcattg aattttggtt ttttccgagg
     1441 cctgctaaag cttaagcaat cgcgcagcgt tctcaccgag aatcattctc ttctgcgcgg
     1501 gagtcatatt cacctgatcg agcacctgca ggggctggtc atagcccatg tccaggcagt
     1561 agtcgctacc gaccatgatg cggtcggcgc ccacttcgct gatcacaaat tccatcacgg
     1621 attttgagtg gacgatggtg tcgtacataa agcgatcaag gtatttgctc ggcgggcgag
     1681 gcaatcgcag cttttggatt tccggacgca cgattgagcc gtggtccatg cggccgagaa
     1741 gcatcagcaa aacgccgccg ccatgcggca gcacaaaccg cagattggga tgccggtcaa
     1801 gcacgcctcc aaaaattaaa tggcacacgg cgattgtggt atcgagcgga aagccaatca
     1861 gattgacgag cgaaaatggc gcgatgcgcg gccctccggc cacctgcaac ggatgcagaa
     1921 agatcggaag atcgagagcc tcgatgcgcg caagaatggg ctcgaatagc ggatcgtcaa
     1981 ggtcgcggtt gttgatattc gttcccatgt acacgccgcg cagtcccggc aatttgctcg
     2041 cgcgattcag ctcgtcgatg gcgcgatctg gaaacatcat gggcagcgtc agcaaaccga
     2101 aaaggcgcgt gggatattgc acgtgcgcgg cactcgccgc gtcgttgtac gcgctggcta
     2161 gtttgtgcga aacctcttcg tctccccaat gcgccatcgg gcccgtgagg gacattgcct
     2221 gcatcgcggt tccctgcgca tccatgtctg caattcgcag tttcaaatcc acagagcggt
     2281 aagggagcgg tccctgaaag cgcacctcgg tcttgatata aaagccttcc tccgcggcgc
     2341 gatattccac gccgaatttc ggcccgtatt cggccaacac atcgaggtag gcctgcggaa
     2401 aaaaatgcgc atgcacatct acaggccgca cgtattttga ttcgtcatta ggcatcgtcc
     2461 gagttctctc ccatcagaac accaaaattt ataatgaaaa ataaatcgcc cacgaaggtc
     2521 aaagcggggt cgtacatgtg attcaaggtt ctgtagtgcg cgccttcggg cgagcattgt
     2581 ggcacaccct acggcaaaca atcgaacact atcaagtgcg cctcttcggt caccagatcc
     2641 gaaagcatcg aagtttccca tcccgcagcc ttatctttct taatttatcc ctgcgcgatg
     2701 tcgtacccat ggaatagcga gaatagcaac acgcgttcat ttcctcgctc ctcacgacga
     2761 aattccggat cgcactcatt ccggtgaccg ttcctgtgtg cgggaaatgt aattcgccgc
     2821 cgcgccctac tcaagcggcg atcttcctga atcgcaattg acgagcgcca gccgatgcgc
     2881 gatgcgatga tatttcaatc tcgcgaaata cgcacatctt tcctcgtaac cgccatcctg
     2941 taggcaaact ctaagggtct tacacaaata gaatgctgga ttcgcagtat tctcgcgcgt
     3001 cgagccactg aactaaggtt ggcgaggcta aagagtgcct gtgcgcacgc ggcacggact
     3061 gtagcgcccg ccttcacgcg ggcaccgggt tgcaatctga tgtccggtag tgggcgctac
     3121 acgaaccaaa tccacgtaat tgcacgggcg ttaacgggaa tcccttgaaa cgtccccgag
     3181 ccgaagtcaa acccccgctt agaaaaggag tcccatgttt ggaaaagtga aatcgttacg
     3241 agaactcttc gatcttgagc tcaattatgc gtatgactgc gagcaaaaac ttgtcgaaaa
     3301 agggctaccc gcgatgatcg aggctgcgac gtctcctcaa ctgcaggcag cccttaaaca
     3361 gcacctggag gaaacgcgcc ttcacgtccg ccgcctggag caggtgttcg gcgccattgg
     3421 caccgaaccc gacacgaaag ataacgaagt actggatgaa ttgatgaatg cggcagagga
     3481 ttccgtttcc aacattgagc aatcggccct gcgtgatgcc gcgctcatcg tgaacggaaa
     3541 catggtggag caccttgaaa ttgccacgta cggcacgctc gccgcttttg cccggaacct
     3601 cggctttcaa aacgtagtca gcgtactgga acagaccctg caggaagaga agacagctga
     3661 tgcgaaactg acgcaacttg ccgaaaccat gaaccagcag gcaaagcgac agtctgcctg
     3721 acgcccagat caatccccta tttaagaagt aggatatacg ctcgacgcgc gccgaggaga
     3781 ttcaatgcca acgaagcgaa aaaccaagca actccgcaag aagaaacccg cacccaagag
     3841 ccgcgcccgc agatcgtcag cgagagatcc gattcggagc ggaacgctca gaagcgctct
     3901 cgatcccact cgtacgaggg aatttcagga aagcggaccg gagactgaag cacccgaaac
     3961 aggcgcttcc gcgggagatc ttgaaggcct ggaaaccgac gacaacgaag attttgaaag
     4021 cgttgcagaa ctggtgagcg aaggccagga tttagaggcc gaagaactcg aaagcataga
     4081 aaaagcgccc aacgcggatc aaggcgactt gaaaccccgc aaggtcccaa gcggcaccaa
     4141 acccggaaaa ttctcggacc gcaaccgcct ctgaaagatg accggagtgt atcgaaacac
     4201 agattcgcga acggtgctgc ggtttctgcc gagcgccgtt cgcactttcg ctcgtcgttg
     4261 gagaatcgaa aaactgaagc gagcccgtag cgcctgctcc catttgctcg ccatacagcg
     4321 ttttatcgcg gttcttagtg caaaactcgc atagttattt cgccctcctg cctgttacag
     4381 ctagaagagt ccgtgcttca ctctcaaggc tcgcgcgcgg gggatcatga aacctgtcta
     4441 tggaaccgcg attctggcaa cgtttttaat ttcgctcgtc atgggcctcc acgcccaacc
     4501 gcaagcaaaa tattccgcag agcaggtaaa acgcggaaaa tatctggtgg aggaagtggc
     4561 gaagtgcccc gagtgccaca cgccgcgcga caaaaataac caattggatc cccaacggtg
     4621 gctgcagggc gcgccaatct ggattcaacc tgtgttcaaa acgccgggct gggcggacga
     4681 agcaccctca ctcgccgggc taacaagcta cacggacgat caagctacgc gagtgttgga
     4741 aaaaggcgag cgagcaaacg gcggcggcac ggtgcaaccg ccgatgcaca tctatcgtat
     4801 gcatcatgag gatgcgcaag ccatcatcgc atatctcagg tccgtgccga atcatccctg
     4861 aacgccgaaa tgattttccg caccgacggc acccacctct gctggaagaa gcgggctagt
     4921 cgggcagccg tgacctggta tgtgcgcggc ctgcaagaac ggagcggcct tgcgttattc
     4981 tttaatcttc caccaatcgt tgcagccccg gtctatcttt tagcgttagc gtcgatccct
     5041 tgatgaccac cagatttttt ttcctgaaaa tcgacaacac gcgggtgaca gtttctctcg
     5101 aggttccaat catttgcgct atttcttcat gcgtcagcgt gagcttcgct ttcaccgcgc
     5161 ctttttcttc tttttgactg gcggtcagat ccagaacgaa gcgggccatt ttctcttccg
     5221 cggaatgcga aagcccgatg gtgcgcattt ccgcaagcgc cgtgtgatac gtctgtccta
     5281 gttgctgcgc cacgcgcaga gccacctcgc cgtattcgcg caaaaaattc aaaaaatcgt
     5341 tcgcggagac aaaattggcc tgggttggct caacaacctc ggccgttagt tcgtacggca
     5401 ttccggtgac cgtcgacggc aaccccagca cttctcctgg ctccgaaagt ctggcaatca
     5461 acgttttccc gtcgattgaa gacgtataga gtttcacccg tccggcgcat aggatgaaaa
     5521 cgcctcgccc agcctgccct tccacaaata atgttgcgcc cttggggtag gacgaaggtg
     5581 aagtgataga cgcgaggcgc tgcactgcgc ctggcgaaag attacaaaac aatcgatctt
     5641 cgcggtgagg gcaggtaagg caactctcaa taatttcaag gccgtacggg gctcgcatag
     5701 ggctgaattc taacgcatgg cccttccccc gcgccaccac atgaaaatga cctaattgcg
     5761 gagcgttgct ttccgtcatg attttcactc ttttgctgcc gttttacgaa ttgtctcccg
     5821 gctaatgaat ctgaaacggc tgcatcttct ttttgaattc tccgatcaac gccgcagaat
     5881 cgttattctg gtctgtcagt gc
//
LOCUS       DASZMB010000131         3805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3434591, whole genome shotgun sequence.
ACCESSION   DASZMB010000131 DASZMB010000000
VERSION     DASZMB010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3805
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3434591"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1019)
                     /locus_tag="VG272_06240"
     CDS             complement(117..1019)
                     /locus_tag="VG272_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HWF13315.1"
                     /translation="MPYATEENLTDLALKQWEKCDSPRLRQIMQSLVKHLHEFAREVE
                     LKPDEWLTAVEWLAKTGQLSSEKRQEFILFSDVLGISMLVDAMNHRFPDGATPSTVMG
                     PFHIEDSPEMKMGANVAEGLSGEPTFLVGTVRDLNGKPIDGASIDIWQADADGLYESQ
                     LGSAEPVLRAIFRTGADGKYAIRTIAPPGYSIPMDGTVGDLLRHTDISHFRPAHIHFL
                     IAAPGYETLITHLFKKDAKYIDSDVVFGVKEPLIVEFEKYAPGKTPTGEILTEPFVVV
                     RYDFTLSKAKVRSESEPALRKVAG"
     gene            1339..2337
                     /gene="galE"
                     /locus_tag="VG272_06245"
     CDS             1339..2337
                     /gene="galE"
                     /locus_tag="VG272_06245"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HWF13316.1"
                     /translation="MAILVTGGSGYIGSVTVECLLAKGEEVVVLDDLAHGHRAALPAN
                     IPFYQGKIGDGDLVSRIAREHKLDSCIHFAAMIEVGESVVDPAKYFENNVAQGIAFAG
                     HLLSAGVKCLVFSSTAATYGDPEEIPITEQSRKWPRNPYGWSKLFMERLFDSYDTAYG
                     LKFVALRYFNAAGATETRGEDHQPESHLIPNVLKAALGHQKAIRVYGNNYPTPDGTPI
                     RDYIHVVDLANAHIRALEYLRAGGSSDYFNLGTGRGYSVLEVIECARQVTGRDIPVQI
                     ESPRAGDPARLIADPTKAKSVLGWEAVASDLRSIVLSAWDWRTRHPGGYGESRPAH"
     gene            2826..3281
                     /locus_tag="VG272_06250"
     CDS             2826..3281
                     /locus_tag="VG272_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13317.1"
                     /translation="MGDSTLKFTPGLREPEIKSTKLGQSPRSIGARQDSVLDERIFHS
                     MLTMERRRAERSRKPYVLMLIDANLDNGASEDILKEAVNVILAVKRETDMVGWYRVGA
                     ILGVIFTEVSVEGKLPVTETLRTKIATALIKNMGVDRSSRIAISMHTEG"
     gene            complement(3436..>3805)
                     /locus_tag="VG272_06255"
     CDS             complement(3436..>3805)
                     /locus_tag="VG272_06255"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HWF13318.1"
                     /translation="ENTSAMTAISNDYGYDHVFSRQLEALAVPGDVAVAISTSGNSPN
                     VVAAILSAQKIGLHTIGLTGASGGKMRELVDTLIAVPSNETPRIQECHILVGHALCDA
                     VEQLLASVPAASGTHARAKA"
BASE COUNT          852 a         1038 c          997 g          918 t
ORIGIN      
        1 gcgggttttc cgccactgaa aactcctccc tcaacgacct tcgtgtagcg cgcgcgttca
       61 ggcgggcatc ctgcacgccg gcacatgcag cctgaaggct ggcgctacaa aacgcgctac
      121 cccgcaacct ttctcagcgc cggctcggat tccgatctca ccttagcttt gctgagcgta
      181 aagtcgtatc tcactaccac aaacggctcc gtcaaaattt ctcctgttgg agttttgccc
      241 ggcgcgtact tttcaaattc cacgatcagc ggttccttca cgccaaaaac cacatcggag
      301 tcgatatatt tcgcatcttt tttaaaaaga tgcgtgatga gcgtttcata gcctggagcg
      361 gcaatcagaa aatgaatgtg cgcgggccgg aaatgtgaaa tatccgtatg ccgcagtaaa
      421 tcgcccaccg tgccgtccat cggaatggaa taaccgggtg gcgcaatcgt gcgaatggcg
      481 tattttccgt ctgctcccgt gcgaaagatc gcgcggagca ccggctccgc cgagcccaac
      541 tgcgattcgt acaggccatc cgcatccgct tgccagatat cgatcgaagc accatcgatc
      601 ggttttccat ttaagtcgcg caccgtcccg acaagaaatg tgggctcgcc ggaaagccct
      661 tcggccacgt tcgcgcccat cttcatctcc ggagaatcct caatgtggaa aggacccatg
      721 accgtggacg gcgtggcgcc atccgggaag cggtgattca ttgcgtcgac gagcatgcta
      781 atccccagca cgtccgaaaa tagaataaat tcctggcgct tttctgagct gagctgccca
      841 gtcttcgcca gccactcgac cgccgtcagc cactcgtcgg gcttaagttc cacttcacgc
      901 gcaaactcat gcagatgttt gacgagcgat tgcatgatct ggcgaagtcg cggagaatcg
      961 cacttttccc attgtttcag ggccagatca gtcaagtttt cttcggtggc atacggcatg
     1021 gcaattccct cccggcaagg atgttggggc gatcttctcg ctgagaagcc ctttttgtcc
     1081 acagctttct gcgtcgctca cacagcggtc ttctcgcgat gtggcgccgc aaatattcga
     1141 ccgcaccagc agcatcgaac tgatgtccaa tcattaaaga tagcgcacct tttcatctac
     1201 acgtactcat gatgaaaaat ccgaagtcaa ccgagtgcaa tgagataaac tcgttttgtt
     1261 atcgtgggca tccagcgaaa tgtttcagcc aattttcgtg cgcaagttaa agatgaaccg
     1321 aggaggaaat gaggactcat ggcaatcctt gtaacaggtg gaagtggcta catcggcagc
     1381 gtcacggtgg agtgcctgct cgccaaggga gaagaggttg tcgtgctgga cgatctggcg
     1441 cacgggcacc gcgccgctct gcccgcgaac attccttttt atcaggggaa aattggcgac
     1501 ggagatttgg tgtcgcgcat tgcccgcgaa cacaaattgg actcctgcat tcattttgcc
     1561 gccatgattg aggtcggcga gtccgtcgtg gatcccgcaa aatatttcga gaataatgtc
     1621 gcgcagggca tcgccttcgc cgggcacttg cttagcgccg gcgtcaaatg tttggtattt
     1681 tcttccaccg cggcgacgta tggcgatcct gaggaaattc cgatcaccga gcagagccga
     1741 aagtggccca gaaatcccta cggctggtcc aaacttttca tggaacgcct cttcgattcc
     1801 tatgacaccg cgtatggatt gaaatttgtg gccttgcgtt acttcaacgc cgctggcgct
     1861 accgaaactc gtggagaaga tcaccaaccg gaatcgcacc tgatacccaa tgttctgaaa
     1921 gccgccctcg gacatcagaa agctatacgc gtttacggta ataactatcc gacgccggac
     1981 ggaaccccca tccgcgatta tattcacgta gtcgatctgg caaatgcaca catccgcgca
     2041 ctggaatatc tccgcgctgg cgggtcgtca gattatttta atttaggaac cggaaggggc
     2101 tattccgttt tggaggttat tgaatgcgcc cggcaagtga ctggccggga tattcctgtg
     2161 cagatcgaat ctccgcgggc gggcgatcct gcgaggctga tcgccgaccc cacgaaagca
     2221 aaatccgttc tgggctggga agcggtggct tccgatctgc gatcgatcgt tttgtccgcc
     2281 tgggattggc gtacgcggca ccccggcggt tacggggaaa gccgtcccgc tcactaaagt
     2341 gcagtagttt ttttataatt cgcggcggct acgtgagtgt cttgtgtgga cgctgactat
     2401 ccctctggtc ttgtcatacc aaactcgatt tcattttggt atagccgagg cgaaacagga
     2461 aatcgttttc acttttccat ccgacgcgaa gcggaggcgt tcggaaactg gaacgctaaa
     2521 gtctcagacg atacgctaag cggtttttta tcagtaaaat gggcgccccc tcctaaagcg
     2581 aatttcaaac gaacgtcagt agcgttctgg cagaccgatt gcagacaatg caatgggttt
     2641 ggtttactat ctctaggctt tcggcaaccc gatgcttttt gagactggct ctttttgaat
     2701 aatcgatttt ggcgtttgac tgaaattcgc acgcctttgc tttgttgtag ggtgttttgc
     2761 ttcggagcgt tcttgcaggc tctgacggca ctacctccgg atgaacgagt cacgcgggag
     2821 aagaaatggg cgacagcact ttaaaattta cgccaggatt gcgcgagcct gagatcaagt
     2881 ccacaaaatt gggtcaaagc ccccgatcta ttggtgcccg tcaagactca gttctggatg
     2941 aaagaatttt tcattccatg ctgacgatgg aacgccggcg agccgagcgc tcccgcaaac
     3001 cttatgtctt gatgctcatc gatgcgaatc tggacaacgg cgcctcggaa gacattttaa
     3061 aagaagcggt caacgtgatt cttgccgtca agcgcgaaac cgatatggtg ggttggtacc
     3121 gcgtcggcgc aatcctggga gtcattttca cggaagtgag cgtcgaagga aagcttcctg
     3181 tcacggaaac gctgcgcacc aagatcgcca ccgcgttgat caaaaatatg ggcgtggatc
     3241 gttcatcgcg gatcgcgatt tcaatgcata ccgaaggata agaaaacgtg gagcggcttt
     3301 ttccatcatc tgcccatacc tcgtaatcca caacttgtta gtctgccgga gccaccgcga
     3361 agtccagtcc ctacattcct tagagtcatt tttttagttt tttgcgttta ttcgattttg
     3421 aagtgattcc gaaattcagg ccttcgcacg ggcatgcgtc ccggacgccg ctggcacgct
     3481 cgcgagcaat tgttcgacgg cgtcgcacaa tgcgtgcccc accagaatgt ggcactcctg
     3541 aattcgcggc gtttcgttcg atggcaccgc gatcagcgtg tccaccaatt cgcgcatttt
     3601 tccgccggat gcccctgtta agccgatggt gtgcaggcca attttctgtg ctgaaagaat
     3661 tgccgccacc acattgggag agttgcccga cgtgctgatt gctactgcta cgtctccggg
     3721 aaccgccaac gcctcaagct gacgtgaaaa tacgtggtca tagccgtagt cattcgagat
     3781 ggctgtcatc gccgaagtgt tttca
//
LOCUS       DASZMB010000132         5432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3686059, whole genome shotgun sequence.
ACCESSION   DASZMB010000132 DASZMB010000000
VERSION     DASZMB010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3686059"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VG272_06260"
     CDS             complement(<1..723)
                     /locus_tag="VG272_06260"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWF13319.1"
                     /translation="MPTNPAPQITNTDPKTGLPRVVIATTAMLSFISFWRAAAIVLND
                     LGSSAFYAGAIAEQAIGKAAPWFILAIMLFAYAVRSMYIESCSMFVRGGVYRVVKEAM
                     GSTLAKFSVSALMFDYILTGPISGVSAGQYLTGFLNELFARFHVSLILPINSTSAVFA
                     IVVTVFFWWENIKGIPESSGKALRIMYVTTVMVVVMIGWCILTVIMRGAHLPPLPSKA
                     TMTFAPEALGWLRFSTLPHTVAI"
     gene            1327..1884
                     /locus_tag="VG272_06265"
     CDS             1327..1884
                     /locus_tag="VG272_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13320.1"
                     /translation="MNILISTLSLAILTALGYTAFRGRRQKQRSAETDLHYGFRPCIG
                     FTRLDGMASLALLLENRFVKPVWAEEIEIYLADLVADTQTTEATLRGIQKIRQMVAPK
                     DTLPISLVEVIYRAAGEPQRKHSSLLTPVLRYRVGEAWFEKRLEVCRIEMMGLTAVKV
                     RRERNAAPANAAPEKIKETATVGAK"
     gene            complement(1942..3891)
                     /locus_tag="VG272_06270"
     CDS             complement(1942..3891)
                     /locus_tag="VG272_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HWF13321.1"
                     /translation="MLSGALLFLCPTDRSISRAESAPDWLVTAGKADLAHFGDGSAAV
                     ILGEWTDFTVDATGKFVMTERRAMRVLNRRSADRFLDAEGQENNAGKVTSIQTWAIAP
                     SGKLTASSKKDIVTVAGFSAFEEFTDVRVKKVSTPGAEDGSLVGFEVVTEGHIPIGGE
                     KFQMEDELPIRQRELHVSVPSGSLHWFVNHPDKVEVVNQSANGALFRTENRSAIIDEP
                     NAPPFTSVAAMVVINYDPKGTGALQSWEQAGLYYHTLFDPGEKPENEIATQVESLAGK
                     QADKVSKIDALYTYVSRQIRYVAIEIGVGGYQPHLPADVYKNKYGDCKDKATLLISML
                     GKIGLRAYPALVGTRGDVEADPKTPTLATFDHMIVALPVPTELRPEIEKLASYDSKNQ
                     ILWMDPTSETDPLGQLPEMDQGVFALIAYPDRGDLQRIPQSPPEQNGFDYTATVHLKT
                     DGTGTADVEAKYLGVSNSRRHMYYRGRSQDEILKTFEGMVARYVSQSAFQKATITGVE
                     DSHQQIVEKFSFSGNFSVASAGDSWFFQPQFLSGLAVPEVGPRPRQLPLDIGTPFHVR
                     CNYREELPPGMRLESVPDKLSVKTEFGELTITYSMNGNVLLASENLSFSQGLISPEKY
                     MEFRDFVNASIRAERQRLRVASSPQ"
     gene            complement(3911..>5432)
                     /locus_tag="VG272_06275"
     CDS             complement(3911..>5432)
                     /locus_tag="VG272_06275"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HWF13322.1"
                     /translation="TFPSVAPGAIIEYELEETVDSLFPPSWIFDTLGIGTMNSSLQVT
                     VGPRLGMAQFPLDNIYAKISVAESSTVRGYQINYNVKNLRPIQDEPYAPPFRDQATMV
                     LFTPTELGFGGDVYPLIKRWDDIADEMKRELGNMEKSEKDARSKAKELADKVSDPRKK
                     AQAIYSYLQQNVKSSNLAGVGLGRTADEILSSKRGDPDEINALYILMLHEAKVDADMV
                     LVATQNWWSLVKAFPNYNQFSRIVTRLNFKDGTGFADPADAAAPFGELPWFDRGVLGL
                     AIKGNKIQDALLPAGTVDENVSTATSTIEVGKDWSLEGDEETDMKGAEAIDFRSMLGN
                     EEPEKLERTLTDIFAYGRSDAEVSKIEHPEFRDSAQPIVLKAHVKERATNELERGGLL
                     LNPWVGDTFERPMFTASVRHSAVRFNFAEKRTSTSIWKLAPEIKVEQLPKDVKIENEL
                     GAFSHSCTQSGATVTCTRAYALKNTLLKTNIEYLNAKKFFEEIAKQDQEVMVLRSE"
BASE COUNT         1234 a         1593 c         1325 g         1280 t
ORIGIN      
        1 gatcgcgacc gtgtgcggca gcgtgctgaa acgaagccag cctagcgcct ctggtgcaaa
       61 cgtcatggtc gctttggacg gcagcggcgg cagatgcgcc ccacgcatga tcaccgtcaa
      121 aatgcaccag ccaatcatga ccaccaccat caccgtagtg acatacatga tccgcaatgc
      181 ttttccgctc gattccggaa tccctttgat gttctcccac cagaaaaaaa cggtcaccac
      241 aatcgcaaat accgcggatg tcgaattgat gggaaggatc aggcttacgt ggaagcgtgc
      301 gaacaactca ttcaaaaatc ccgttagata ctggcccgcg gagacgccgc tgatgggccc
      361 tgtcaaaatg taatcgaaca tcaacgccga cacggaaaac ttcgccagcg tgctgcccat
      421 ggcctctttg actaccctgt acacaccgcc gcgcacaaac atgctgcagc tctcgatgta
      481 catcgaccgt acggcgtatg cgaacaacat gatcgccaga atgaaccatg gcgctgcctt
      541 cccgatagct tgctccgcga tggcccccgc gtaaaacgca gacgaaccca aatcattcag
      601 aacgatcgcc gccgctctcc aaaaagaaat aaacgaaagc atagccgtgg tggcaatgac
      661 gacccgagga agtccagttt tcggatcggt attcgttatt tgtggtgccg gattagttgg
      721 catatctttg ctgggttaag aaccttggcg acacacacgg tgcgcgggcg ctttctcctt
      781 ttcaaaaaag caaacggctt ggccgatctc tgcacacata tcggcggagt gcccacgctt
      841 cctcacgcag aactctgcgg gatgctccga ggatcgttct ttatggttat cggccaacct
      901 gcaccccctg caacaattga ccgcctaaat aaatacatta aatttcataa ggaactcata
      961 aagaggctcg cattttttcg gcggaaaact gccccaatca ccctatctag ttgctaatcg
     1021 ccctgttatg aagtggttcc gcatgctgct ccattgtttc cgagataggg tttttgtccc
     1081 acgaatcacg cgtcgatcag gagtgcggcc cggcccgtac ttcctattta cattattgaa
     1141 tataacggct atatatacat tgcgtaacaa tatgagcctc gcggacgaca gagctcagtt
     1201 taatttcaat tgatggttac gcgcagggaa acagcatcgt aaggcgatat tcgtcgcatg
     1261 tttaaaaatg ttggcctgct ggtccagaat ggtttcggtt aaaattttca ggtccttctt
     1321 attccaatga atattcttat ttcgacttta tccttggcta ttctgaccgc gcttggttac
     1381 accgctttcc gtgggcggcg acaaaaacaa cgctcggcgg agacagatct tcattacgga
     1441 tttcgtccct gcatcggttt tacgcggctt gacggtatgg catcgcttgc ccttttgctg
     1501 gaaaatcgat tcgtcaaacc tgtctgggcg gaagaaatcg aaatttacct cgcggacctc
     1561 gtggcggaca ctcaaaccac cgaagctact ctgcgcggaa ttcaaaaaat tcggcagatg
     1621 gtcgcgccca aggacactct acccatcagc ctggtggaag tgatctacag agccgccggc
     1681 gagcctcagc gcaaacactc aagcctgctg actcccgtgt tgcgctatcg cgtgggcgaa
     1741 gcctggtttg aaaaacgcct cgaggtttgc agaattgaaa tgatgggcct taccgcggtt
     1801 aaagtccggc gcgagcgcaa tgcagctcca gcaaacgctg ctcctgaaaa aattaaggag
     1861 actgctacgg tcggcgctaa gtgaaaatca ctgcgcaaga ccgtttccct gcctgctgcc
     1921 aaaactcaag atcaggcaaa gtcactgcgg ggaacttgcc acccgaagac gctgccgctc
     1981 cgcgcggatc gacgcgttta caaaatcgcg aaactccatg tacttttccg gtgaaataag
     2041 tccctgcgaa aaggaaagat tctccgacgc cagcagcacg ttgccattca tagaatatgt
     2101 gatggtgagc tcgccaaact cagtcttcac cgacagtttg tccggaacac tctcgagtcg
     2161 catccccgga ggcaattcct cccggtaatt acatctcacg tgaaacggcg tcccgatatc
     2221 caacggcaac tgtcggggtc tcgggccaac ttccggcacg gccagcccgc tcaaaaactg
     2281 cggctgaaaa aaccagctgt ctcccgcgga agcgacagaa aaatttcccg agaaagaaaa
     2341 cttctcgacg atttgctgat ggctgtcctc gactcccgtg atcgtagcct tctggaacgc
     2401 ggactgactc acgtagcgcg cgaccattcc ctcaaaagtt ttgaggattt catcctgtga
     2461 gcgcccgcga taatacatat gccggcgcga attggatacc ccaagatatt tcgcctcaac
     2521 gtccgcggtt cccgttccgt ccgttttcaa atgcactgtc gccgtgtaat cgaaaccatt
     2581 ttgctccgga ggagattgag gaatccgctg caggtcacct cgatccggat acgcgatgag
     2641 cgcgaacacg ccctgatcca tttccggaag ctggcccagc ggatcggtct ctgaagttgg
     2701 atccatccac aaaatttgat tctttgaatc gtacgaggcc agtttctcaa tctcgggccg
     2761 caattcggtg ggcaccggca gggccacgat catgtgatcg aacgtggcaa gcgtgggagt
     2821 tttgggatcg gcctccacat ctccacgcgt gccaaccagc gccggataag cgcgcaaccc
     2881 aattttgccc agcatggaaa tcagcagcgt cgccttgtct ttgcaatctc catatttatt
     2941 tttgtacacg tcggcaggca ggtgaggctg gtagcctccc accccaattt caatcgccac
     3001 gtaccgaatc tgacgcgaga cgtaagtgta cagcgcatca atcttggaaa ccttgtcggc
     3061 ctgttttcct gcgagcgact cgacctgcgt ggcaatctcg ttttcgggtt tttcgccggg
     3121 atcgaacaac gtgtggtagt acaggcccgc ttgctcccag gattgcaatg cgcccgttcc
     3181 ttttggatcg tagttgatca ctaccatcgc ggcgacggaa gtaaacggag gcgcattcgg
     3241 ctcgtcaata atcgcgctgc gattttccgt gcgaaataat gctccgttcg ccgactgatt
     3301 caccacttcc actttgtccg gatgattcac gaaccagtgc aacgaacctg aagggaccga
     3361 tacgtgcaat tctcgttggc ggatcggaag ctcgtcctcc atctgaaact tctctccgcc
     3421 aatcggaatg tggccttccg tcactacctc aaatccaacg agcgacccgt cctccgctcc
     3481 aggcgtgctg acctttttta cccgcacgtc ggtaaactct tcaaacgcgc taaatccagc
     3541 tacggtcaca atatcttttt tcgaggacgc agtgagcttg ccgctgggcg cgatcgccca
     3601 ggtctgtatg gacgtcacct ttcctgcatt attttcctga ccctccgcat ccagaaagcg
     3661 gtccgcggaa cggcggttca gcacgcgcat cgcgcggcgc tccgtcatta caaatttccc
     3721 ggtcgcgtcc accgtgaaat ccgtccactc gcccaaaatt acggcggcac ttccgtcgcc
     3781 aaaatgggcc agatccgcct tgcccgccgt cacaagccag tccggagcgg actccgcgcg
     3841 actgatcgac ctgtccgtcg ggcacaaaaa tagcagagcg cctgacagca aacatgctgc
     3901 gacggaacga ctattcagag cgcaggacca tgacttcctg gtcctgcttg gcgatttctt
     3961 caaagaattt cttggcattt aggtactcga tattcgtttt tagaagagta ttttttagtg
     4021 cgtacgcacg cgtgcacgtc accgtggctc cgctctgcgt gcaagaatgc gaaaacgcgc
     4081 ccaactcgtt ttcaattttc acatccttcg gcaactgctc cactttgatt tccggcgcaa
     4141 gcttccagat ggaggtactc gtccgttttt cagcaaaatt gaaacgcacc gcggagtgcc
     4201 gcacgctcgc cgtgaacatt ggccgctcaa acgtatcccc cacccacgga tttagaagca
     4261 ggccgccgcg ttcgagctcg tttgtcgctc gttctttcac gtgtgctttc agcacaatcg
     4321 gctgcgccga atcgcgaaac tcaggatgct cgatcttgct tacttccgcg tcggaccttc
     4381 cataggcgaa aatgtcagtc agtgttcgct ccagtttttc cggttcttca ttgcccagca
     4441 tcgagcggaa atcgatcgct tcggcgcctt tcatgtccgt ttcttcatca ccttccagtg
     4501 accagtcttt gccgacctca atggtgctcg tcgcggtgga aacattttcg tccaccgtcc
     4561 cagcaggcag cagcgcgtcc tgtattttat ttcccttgat ggcaagcccc agcacacctc
     4621 ggtcaaacca gggcagttct ccgaacggcg cagcggcgtc cgccggatcg gcgaatccgg
     4681 tcccatcctt gaaattcagc cgcgtgacga ttctcgaaaa ttggttgtaa ttgggaaacg
     4741 ctttcaccag cgaccaccag ttttgagtgg ccaccagcac catgtccgca tccacctttg
     4801 cttcatgcag cattagtata taaagagcgt tgatctcatc cggatcgccg cgtttcgacg
     4861 aaagaatttc gtccgcggtc cggcccaagc ccactccagc caagttgctc gacttgacgt
     4921 tctgttgcaa gtaagaataa atagcctgcg cttttttccg tgggtcggaa actttatcgg
     4981 ctagttcctt cgccttgctc ctggcatctt tctcgctttt ttccatgttc cccagttcgc
     5041 gcttcatttc atcggctatg tcatcccatc tttttataag cggatacacg tcgccgccaa
     5101 agcccagctc ggtgggagtg aagagcacca tggtcgcctg atctcgaaaa ggcggagcat
     5161 aaggctcatc ttgaatggga cgcaaatttt tcacgttgta gttgatctga tagcccctca
     5221 ccgtggacga ttccgcaacg ctgatcttgg cgtagatgtt gtccagcgga aattgagcca
     5281 tgccgagccg cggacccacc gtcacctgca gcgaggaatt catcgttccg attcccaggg
     5341 tgtcaaaaat ccacgacggc ggaaacaaac tatcgaccgt ttcttcgagc tcgtactcga
     5401 taatcgctcc gggcgcgacc gagggaaacg ta
//
LOCUS       DASZMB010000133         4586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4521151, whole genome shotgun sequence.
ACCESSION   DASZMB010000133 DASZMB010000000
VERSION     DASZMB010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4521151"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VG272_06280"
     CDS             <1..485
                     /locus_tag="VG272_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13323.1"
                     /translation="ATPAAVGSSKLGVSQHNRVPLGPLASSLAVLSLSHLEGFADYKK
                     HRTRCTFFGTIKFSLIRADRLDVSEKRIEQRIPLMARVDILWMDNELTPWIAPAILED
                     RSRGGVSLRMKNAVQVGAHVTVKSGMLQYSGIVMNCRRDNGTYITGIKLDPREEPEDK
                     "
     gene            complement(571..2157)
                     /locus_tag="VG272_06285"
     CDS             complement(571..2157)
                     /locus_tag="VG272_06285"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HWF13324.1"
                     /translation="MRIQIKFLAALVLAIAISSVGYGATISGTVNGADGAPFQGAFVE
                     AQNSKTKITVIVLSNSQGKYRIPDLTAGDYRVQIKSAGYRAEPRNPVTLSQDQKASLD
                     FSLQKGPVRWNEISQNQAAQLWPASKGKDLLFGHCNICHQFQSRIASVGRDLDGWKDR
                     VAFMRQAMHFSIFLGPNFSDQDAEVVATYLDSLFGPDATLPKSPADMPGYQKTVRPVG
                     NDALNIVYVEYDMPAPSRMPFSAAPDKHGFLWIPDFGLANKITRLDPKTGEMKDFSIP
                     FGGTGGVHSAVPAPDGSVWLAEQGSNRIGRWDPATEKIAEFQDAYLPGKEGIEDGGSK
                     HTVRVEPSGKVWTSGVPLTRFDPETNKFERFVEGVYAYDVKLDKNGDAWFTDPLVNKI
                     GRVNGKTMKVSQWTSPTANSHPRRMEIGSDGMIYSGEYNGGKMARFDPKTETFKEFPL
                     PGPDASPYAMGFDADGYLWYDSHNMDTINRFDPRTGKVIEYPFPHSELCMREFFRDAQ
                     GHMWYGTAPNNKVGYFYLAK"
     gene            2396..4186
                     /locus_tag="VG272_06290"
     CDS             2396..4186
                     /locus_tag="VG272_06290"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWF13325.1"
                     /translation="MKKDRPDRSRFSRRDFIKTTATGAGAAAITGFGIEAAKAEGRPA
                     KWDKEADVVIIGAGATGLPAAIQAAEGGATVILVEANSDVGGHAILSGGNVALGGGTS
                     RQKKYGVVDSPDLLFSDLTDWSVVEPNGFPDYRYNDKEIIRAFADESAKTYEWLVEHG
                     VTFVEKAPDTAGGHACGNSAPRESHSAPMAYTLVQNGGPTPPEKAATTSSGVGLIRPL
                     EASARKLGVQILLQHKMTGIVRENPTSGRVLGISAITQGKTVNIRARKGVIIGSGGHS
                     GNVNFRRIFDPRLTEEYQVAGEPYSFQDGSGEIAAMAVGASLWGAFNQTGEFGQNITK
                     AGWIGCQYGYINLTWQPDSPIFAKARASGLFVKDYQDAILVNIAGLRFYDETQGQFPA
                     NNYNSIKSYVPRSYLNAANLKYEPSNFINAALAGTGDSTPGGGPIWAVFDADAVKREA
                     WKVTPPFVDTKEGYFFSADTLPELAEKIVNKFQKKPMPPNALQETVTRYNSFVDAGKD
                     TDFGKPAPKYKIQTAPFYAAWATPVIHDTRSGLRINAKCQVMDMNGEVISGLYCGGES
                     AGGFSLHGLARCAVQGRIAGRNAASETAKA"
BASE COUNT         1073 a         1268 c         1273 g          972 t
ORIGIN      
        1 gcgcaactcc cgctgcagtt ggctcatcaa aactaggcgt cagtcaacac aatcgggtcc
       61 cccttggccc actagcttcg tcgcttgccg ttttaagtct ttcgcatctt gaaggtttcg
      121 ctgattacaa aaagcaccgg acgcgatgca cgttcttcgg aacaataaag ttctctctaa
      181 ttcgtgccga taggcttgac gtgagcgaaa aaagaatcga acaacgtatc cccctgatgg
      241 cgcgggtcga tattctgtgg atggataatg aactgacccc gtggatcgcg ccggccattt
      301 tggaggatcg gtcgcgcggc ggagtgagtt tacggatgaa aaatgccgtc caagtaggtg
      361 cgcacgtcac ggtgaaatcg ggaatgttgc agtattccgg aatagtaatg aattgccgcc
      421 gcgataacgg cacctatatc accggcatca aactggatcc ccgcgaagag cccgaagata
      481 aataggtttt aagtagcaca ggtttcagcg ggtggaattt agtcttcgca agggcaaaac
      541 cccacaggct ggagcctgtg ctacctaaat ctacttcgcc agataaaaat agccgacttt
      601 attgttggga gccgttccgt accacatgtg tccctgcgcg tcgcggaaaa attcgcgcat
      661 gcatagctcg gaatgtggaa acggatactc gatcactttt cctgtgcgcg gatcgaagcg
      721 attgatcgta tccatgttgt gcgagtcata ccaaagatag ccgtccgcgt cgaagcccat
      781 cgcgtagggg ctcgcgtccg gaccgggcag cggaaactct ttaaacgttt ctgtctttgg
      841 atcgaatcga gccattttgc cgccattgta ttcgcccgag taaatcattc catccgagcc
      901 aatttccata cggcgcggat gcgaattcgc ggtgggcgaa gtccattgcg aaaccttcat
      961 cgttttccca ttgactctcc cgattttatt gaccagcgga tcggtgaacc acgcgtcgcc
     1021 gtttttatcg agcttcacgt cgtaagcgta cacaccttcc acgaagcgct caaatttatt
     1081 cgtttcagga tcgaagcgtg tcagcgggac gccgctcgtc cacacttttc cgctaggctc
     1141 gacgcgcacg gtgtgcttcg agccgccgtc ttcaattccc tctttacctg gtaaatatgc
     1201 atcctgaaat tccgcaattt tttccgtcgc cggatcccac cggccgatgc ggttggaacc
     1261 ttgctcagca agccacacgg agccatccgg cgcgggcaca gccgaatgga cgccgccggt
     1321 cccgccaaac ggaatggaaa aatccttcat ttcgccggtt tttggatcga ggcgcgtaat
     1381 tttgttggcg aggccgaaat cggggatcca cagaaaaccg tgtttatcgg gagccgcgct
     1441 gaacggcatg cggctcggcg cgggcatgtc gtactcgaca taaacaatgt tgagcgcatc
     1501 gttgccgacg ggacgcacag tcttttggta gccaggcatg tccgcgggag acttgggcag
     1561 ggtggcatcg gggccgaaca agctgtcaag gtacgtcgct acaacctccg cgtcctgatc
     1621 gctaaagttc ggtcccagaa aaatgctgaa gtgcattgcc tgccgcatga atgcgacgcg
     1681 gtctttccag ccatcgaggt cgcgacccac ggaagcgatg cggctctgaa attgatggca
     1741 gatattgcag tggccaaaca acaagtcttt gcccttggac gcgggccaga gttgcgccgc
     1801 ctgattctgc gaaatttcat tccagcgcac cgggcctttt tgcaaagaaa aatcgagcga
     1861 agctttttga tcctgcgaaa gtgtgactgg gttgcggggt tccgcgcgat agcctgctga
     1921 cttaatctgt acgcgatagt cccccgcagt tagatcggga atgcgatatt ttccctggct
     1981 gttggaaagc acgatgaccg taattttcgt tttggaattc tgggcctcga cgaacgcgcc
     2041 ctgaaatggc gcgccgtcag ccccgttcac cgttccggaa attgtcgcgc cgtagccaac
     2101 cgaactgatc gcaatggcca agaccaaagc cgccagaaat ttaatttgta tgcgcacaag
     2161 tgtctccggt gttggaagtg gcccgggctt cagcctgcgg ggtttagtcc tcgcaaggcc
     2221 ccgaattccg cgggctgaag cccgcgccac ttaaaaccaa tcctagcttt tttcctaatt
     2281 gcaggcacta caattcctcg cgtcacagag ttcgctgcgc ggttgacacg tttgctcaaa
     2341 cggcatatct tcatgcgcaa ggcactgaga acaaaggcac attggatcac aaaaaatgaa
     2401 aaaagacagg ccagaccgaa gtcgtttttc acgccgcgat ttcatcaaga cgaccgccac
     2461 aggcgcgggt gcagcggcca tcacgggatt cggcatcgaa gctgccaagg ccgaaggccg
     2521 cccagcgaag tgggacaagg aagcagacgt cgtgattatc ggcgctggtg cgacagggct
     2581 gcctgccgcc attcaagcgg ccgaaggcgg cgccaccgtc attcttgttg aagcgaactc
     2641 ggatgtcggc ggacacgcga tactcagcgg cgggaacgtg gcgctgggcg gtggcactag
     2701 ccgccagaaa aaatacggtg tcgtcgactc gcctgatctt ttattttcgg acctcacgga
     2761 ttggtccgtc gtcgagccga atgggtttcc ggactaccgt tataacgaca aggaaattat
     2821 tcgggcgttt gccgacgaaa gcgccaagac gtacgagtgg ctcgtcgagc acggcgtcac
     2881 gttcgtcgaa aaagcgccgg atacggctgg cgggcacgcc tgcggcaatt cggcgccgcg
     2941 cgaaagtcat tccgctccca tggcctacac gcttgtgcaa aatggcggcc ccacgcctcc
     3001 cgaaaaagcg gcgaccacgt cttcgggcgt gggccttatc cgaccgctgg aggccagcgc
     3061 gcgtaaattg ggcgtacaaa ttcttttgca acacaaaatg acgggcatcg ttcgtgaaaa
     3121 tccaacgtca ggaagagtgt tgggcatttc cgcaatcacg cagggaaaga ccgtaaatat
     3181 tcgcgcgcgc aagggcgtga tcatcggctc cggcgggcac agtggcaacg taaatttccg
     3241 ccgcattttt gatccgcgcc tcaccgaaga ataccaagtg gctggcgagc catactcctt
     3301 tcaggacggc agcggcgaaa tcgccgcgat ggcggtcggt gcatcgttgt ggggcgcatt
     3361 caatcagacg ggcgaattcg ggcagaatat taccaaggcc ggatggattg gctgccaata
     3421 tggctacatc aatttgacat ggcagccgga cagcccgatc tttgcgaagg cacgcgcttc
     3481 gggccttttc gtaaaagatt accaggacgc gattctcgtc aacatcgcgg gactgcggtt
     3541 ctacgacgaa actcagggac aattccctgc caacaattac aactccatta aatcgtacgt
     3601 gccgcgcagc tacctgaatg ccgcgaatct gaagtacgag ccatccaact tcatcaacgc
     3661 agctctcgcc ggaaccggag attcaacccc aggtgggggg ccgatctggg ccgtctttga
     3721 tgcggacgcg gtgaagcgcg aagcttggaa agtgacaccg ccgtttgtgg acaccaagga
     3781 aggctacttt ttttccgccg atacactccc tgaactggcc gagaaaattg taaacaagtt
     3841 ccagaaaaaa ccgatgccgc cgaacgcgct gcaagaaacc gtcacacgct acaactcttt
     3901 cgtcgatgcc ggcaaggaca ccgatttcgg caaacccgcg ccgaaataca aaattcaaac
     3961 ggctcccttt tacgcggcgt gggccactcc ggtgattcat gacacacgct caggactccg
     4021 catcaacgca aagtgccagg tgatggacat gaacggcgag gtcatttccg gcctatactg
     4081 cggtggagag tctgccggcg gattcagcct gcatggcctg gcccgatgcg cggtgcaggg
     4141 ccgaattgct ggcagaaatg cggcctccga aactgcgaag gcataagacg acatcgtgat
     4201 gtgcgtcgat cgaaacaccc ctcattccag tgacgcatta tccagctagc gcctaatcct
     4261 tttgccgcaa aaacgcatga aagtaaaaat taccccatcg aacgaagctt cgaaaagctc
     4321 ctttatgggg aggatgaacg ttgctgaagg cggtattttg gtctgagtcg tgatcgaaat
     4381 attttttgcg attgcttcgg tcctcccgca ggacgcgcgt tcacaatccg attgagtcgg
     4441 cgagcgagga aaaggaggaa tagtaggatg cgcagagatg ttcgcaatag gaaaacgcgc
     4501 cttccgagaa gggtgatgag cgacgcagca agcaggagga ccgcatgaca cactctctcc
     4561 ccgacggact cacatcgatt ctcagc
//
LOCUS       DASZMB010000134         4519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3984226, whole genome shotgun sequence.
ACCESSION   DASZMB010000134 DASZMB010000000
VERSION     DASZMB010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3984226"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VG272_06295"
     CDS             <1..211
                     /locus_tag="VG272_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13326.1"
                     /translation="FVGKFGDWVKLKNSWNKRLKRDGLTYFRATEYYSLRGEFAKYRD
                     PINDGSMRKGVGQVLEPYTSSVLLR"
     gene            579..2372
                     /locus_tag="VG272_06300"
     CDS             579..2372
                     /locus_tag="VG272_06300"
                     /inference="COORDINATES: protein motif:HMM:NF015530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13327.1"
                     /translation="MARKPANIPDMLAFLRVRRALHPIVFVCCGLIALILFPLEYFTV
                     SAQDHSASRTSTHEKSNTNAKNLVIAPDLSQEVAKFKPVKMPFDMTHLSERERQMMAK
                     LVEACQNLESIYWRQSDPDGLQLYHSLAGSTAPTDAAALRRFLRINGSRYDLIKNNTP
                     FVGTAPWPPGRNIFPWGITREEFDRYVAAHPEQKEALYNPFTVVRRKENKLETIPYHV
                     AYKQWVDPAAKALREAATFSDDPAFANFLRMRADALQSDKYFESDIAWVSLVNPKFDI
                     IFAPYETYLDDLLGVKTSYGAAILVRNETESRKLEVFQKYVPDIQDSLPLAAEDRPSK
                     RGLFSPMEVMDTPYRAGDLRHGYQAVADNLPNDPRIHEQKGSKKIFFKNFMDARVNYV
                     ILPIAKRLLREDEAALASAEGYLASTMMHEISHGLGPAFARTASGRRDVREAIGPTYS
                     ALEEAKADIVGLYGLKWLVDRGDLPKEKLNGYYASEVAGIFRTVRFGVAEAHGRAEIM
                     EFNFYSERGAISRDPASGRYAIDFARMPDAVAALAKELLEQEATGDRARVEAWFAKYG
                     TIPPELSKALEAVSDVPVDIDPISIFPEPMR"
     gene            2407..2844
                     /locus_tag="VG272_06305"
     CDS             2407..2844
                     /locus_tag="VG272_06305"
                     /inference="COORDINATES: protein motif:HMM:NF040124.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6526 family protein"
                     /protein_id="HWF13328.1"
                     /translation="MANEKTQNFQNHTRQVPVFAAGQIILMVGFVGRIVALRHGVSFQ
                     SVMDVLMIAALIVLFVNGRNSTVTVQNRLIRLEMRLHLARLLPADLQPRIGEFTVDQL
                     IALRFASDAELPALARKVLDDKMNDRKPIKQLVKDWQADWLRA"
     gene            complement(2975..4081)
                     /locus_tag="VG272_06310"
     CDS             complement(2975..4081)
                     /locus_tag="VG272_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWF13329.1"
                     /translation="MTIPHRILCTVAGIFTLTAAAAFLVPSAGAQQSANGTWRFLASG
                     DSRNCGDVVMPGIAETARKNQVAFYWHLGDLRWTTNFDEDIVHQPEHLTKPMTVTEYE
                     SIEWPDYIQSQITPFGSIPFFLGIGNHETVPPYKTREEFLLQFADWLNSPVLRAQRLK
                     DDPSDYRMKTYFHWIDRGVAFYYLDSATADQFDAAQLRWFERVLAKDLVDPAVTTIVT
                     GMHKALPESISAGHSMNESPTGTESGRRVYADLLRARDEAHKHVYVLASHSHYYMDGI
                     FNTEYWKQHGGVLPGWIVGTAGAVRYALPPNSSDAREALVNVYGSLLGTVQPDGEINF
                     EFQKLEESDVPAQITARYGKDFVHWCFAENTQAK"
     gene            complement(4270..>4519)
                     /locus_tag="VG272_06315"
     CDS             complement(4270..>4519)
                     /locus_tag="VG272_06315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13330.1"
                     /translation="WFSPYLMDAPGETPMRIIHESYTPDGLWNLVYDEPPDWKQVQSD
                     YDYVWAYDVPKFSASLATIAERIYTSGPLEVYRIRKSP"
BASE COUNT         1050 a         1173 c         1270 g         1026 t
ORIGIN      
        1 cttcgttgga aagtttggcg attgggttaa actgaaaaat agttggaata aacgtttgaa
       61 aagagatggg ctaacgtatt ttcgtgcaac agagtactac agtttgcgcg gggaatttgc
      121 aaaatatcgt gatccaatca atgatggatc aatgcgtaaa ggtgtcggtc aagtactgga
      181 gccgtacaca tctagcgttc ttttgcgatg aggcaccgaa ttcaaaacga atttatgacg
      241 tttatttagc ctataaatcc gtaaacccag aatcaggcgc tctaatagag tcttttgttc
      301 atcgagatga taaggtattt gcaccgttgc aagttgccga tttaatagcg catttaggcc
      361 gcgaactcta tgggactaaa aaagaacctg atagaatccg gaaagcgata ggatggatca
      421 aggtatggga cgaggcttat atgcttgcac ttttcgagca tgagaaaatg cgacgaaacc
      481 tcaagtagtg cccgcctgtc accttagaag cctagcacag tttggttagc catgtatatt
      541 attgcccttg caaggcctaa acccacaagc tgaagcctat ggcacgcaaa cctgctaata
      601 ttccggacat gcttgcgttt ctacgcgtcc gtcgtgcact tcatccaatt gtttttgtgt
      661 gttgtgggct gatcgcatta attttatttc ccctcgaata cttcaccgtt agtgcgcagg
      721 atcattccgc gtctcgcaca agcacgcacg agaaatctaa cacaaacgcg aaaaatcttg
      781 tgatcgcgcc ggacctttcg caggaagtgg caaaattcaa gccagtgaaa atgccgttcg
      841 atatgacgca cctgagcgaa cgcgagcggc agatgatggc gaagctggtg gaggcgtgcc
      901 agaatctgga atcaatttac tggcggcaaa gtgatcctga tggtttgcag ctttatcatt
      961 cgctcgccgg cagcacagcg ccgacggacg ctgctgcgct gcgccgattt ttgcgcatca
     1021 acggcagccg ctatgacctg atcaaaaata atacgccatt cgttggcacc gcgccgtggc
     1081 cgccggggcg caacattttt ccatggggca tcacgcgcga agaattcgac cgctacgttg
     1141 ccgcgcatcc cgagcagaag gaagcgctgt acaatccgtt cacggtggtt cgccgcaaag
     1201 aaaacaagct cgaaacgatt ccctatcacg tggcgtacaa gcagtgggtc gatcccgcgg
     1261 cgaaggcgct gcgcgaggcc gcaacttttt cagacgatcc ggcattcgca aattttctgc
     1321 gcatgcgcgc ggacgccttg cagtccgaca aatattttga gagtgacatt gcctgggtca
     1381 gcctcgtcaa tccgaaattc gatattattt ttgcgcctta tgaaacctat ctggatgatt
     1441 tgctcggcgt gaaaacttct tacggcgcgg ccatcctcgt gcgcaatgaa accgagagcc
     1501 gcaaactgga ggtattccag aaatatgtgc cggacattca ggacagtttg ccgctcgcag
     1561 cggaagatcg cccctcgaaa cgagggcttt tttctccgat ggaagtgatg gacacgccgt
     1621 atcgcgcggg tgatttgcgc cacggctatc aggcggtggc cgacaatctg ccgaatgatc
     1681 cgcgcattca cgagcaaaaa ggctcgaaga aaattttctt caagaatttc atggacgcac
     1741 gcgtaaatta cgtcatcctg ccgattgcaa aacgcctatt gcgagaggat gaagcggcgc
     1801 tcgcttccgc cgaaggctat cttgcgtcca ccatgatgca tgaaatttcg cacggcctcg
     1861 gccccgcgtt cgcgcgaacc gcaagcgggc ggcgcgacgt tcgtgaagcg attgggccaa
     1921 catattccgc gctggaagaa gcgaaagctg atattgtggg tctatacgga ctgaaatggc
     1981 tcgtcgatcg cggagatttg ccaaaagaga aattgaacgg ttactacgca tcggaagtgg
     2041 ccggaatttt tcgcacggta cgttttggcg tggcagaggc ccacggacgc gcggaaatta
     2101 tggagttcaa tttttactca gaacgcggcg ccatttctcg tgatcccgct tcgggccgct
     2161 atgcgattga cttcgcacgc atgccggatg cggtcgcggc gctcgcaaag gaattgctcg
     2221 agcaggaagc gaccggagac cgcgctcgcg tcgaagcgtg gttcgcaaag tacggcacga
     2281 ttccgccgga actatccaag gcgctggaag ccgtgtccga tgttccggtg gatatcgatc
     2341 ccatttccat ttttcccgaa cccatgcgct aaacattcca attcgcaaac gaaaggaata
     2401 gaaaaaatgg caaacgaaaa gacgcagaac tttcagaatc acacacggca agtgccggtg
     2461 ttcgcggccg ggcaaatcat tcttatggtg ggcttcgtcg ggcggatcgt tgcactgcgg
     2521 cacggtgtat cgttccagtc cgtgatggac gtgctgatga ttgcggcact gatcgtgctg
     2581 tttgtaaatg gtcgaaattc cacggtgacg gtgcagaacc ggctgatccg ccttgaaatg
     2641 cgcttgcacc tcgcgagatt gctgcccgcg gatttgcagc cgcgcattgg cgaattcacg
     2701 gtcgatcagc tgatcgcgct gcgatttgca agcgatgcgg aattgcccgc gctggcgcgc
     2761 aaagtgctcg atgacaaaat gaacgatcgc aagccgatca agcagttggt gaaggactgg
     2821 caggcggatt ggctgagggc gtagattttt gtggcatagc tttttgtggc gcgggcttca
     2881 acctgcggaa tttgggcctt gcacattttc aacccagagg ggttgagtcc atgcaaggcc
     2941 caacccgtgc aggcgctaaa gcccgcgcca ctttttactt cgcctgcgta ttttcggcga
     3001 agcaccagtg gacgaaatct ttgccgtaac gcgcggtgat ttgcgcggga acatcgctct
     3061 cttccaactt ttgaaactcg aaattgattt cgccgtccgg ctgcaccgtt cccaacagcg
     3121 aaccgtagac gttcaccagc gcctcacgtg catccgatga atttggcggc aacgcgtagc
     3181 gcacggctcc ggccgtgcca acgatccagc caggcagaac gccgccgtgc tgcttccagt
     3241 actccgtgtt gaaaattccg tccatgtaat agtgcgaatg gctggcgagc acgtagacat
     3301 gtttgtgcgc ttcgtcccgc gcgcgcagca aatccgcgta aacgcggcgg ccgctttcgg
     3361 ttcctgtcgg cgattcgttc atgctgtgac cggcggagat gctttcgggc agcgccttgt
     3421 gcattccagt aacgatggtg gtgaccgcag gatcgacgag gtctttcgcc agcacgcgct
     3481 caaaccagcg caattgcgca gcgtcaaact gatcggcggt ggcgctatcg aggtaataga
     3541 atgcgacgcc gcgatcgatc cagtgaaaat aagttttcat tcggtagtcg gagggatcgt
     3601 ccttcaatcg ctgcgcacgc aacaccggcg aattgagcca atccgcgaat tgcagcagaa
     3661 actcttcgcg cgttttgtac ggcggaacgg tctcgtgatt tccgattccc aaaaaaaatg
     3721 ggatggagcc aaagggcgtg atctgacttt gaatgtagtc aggccattcg atactctcgt
     3781 attcggtgac cgtcatcggc ttagtgaggt gctcgggctg gtgcacgatg tcttcgtcga
     3841 aattggtggt ccagcgcaaa tcgcctaaat gccagtaaaa tgcgacctga ttctttcggg
     3901 cggtctctgc gatgccgggc attacgacgt caccgcaatt gcgcgagtcg ccggaagcga
     3961 ggaagcgcca cgtgccgttc gcggattgct gtgcgccagc actgggcacc aggaatgccg
     4021 cagccgctgt gagcgtgaaa atgccggcga ccgtgcacaa aattctatga gggattgtca
     4081 ttttcaatgt aagctccgcg caccaatgtt agcagaccgt ctttccgcca tgtgtgaaca
     4141 attcttaaca ttggatacgg atttcagtgg cgcgggtctc agcctctggg gttggggcct
     4201 tgcaagaact gaaccccttg gattgaaagc gcgccaggac caaattccgc agcccgcgcc
     4261 acagcaaagc tacggcgatt tgcgaatgcg gtaaacttcc agcgggccgg aagtgtaaat
     4321 gcgctcggcg atcgtggcca gtgaagccga aaattttggc acgtcgtagg cccacacgta
     4381 atcgtaatcg ctctgcacct gcttccaatc gggcggttcg tcgtagacga ggttccataa
     4441 gccgtctggc gtgtaggatt cgtgaatgat gcgcatgggc gtttcgccgg gcgcgtccat
     4501 caggtacgga gaaaaccag
//
LOCUS       DASZMB010000135         6041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2882946, whole genome shotgun sequence.
ACCESSION   DASZMB010000135 DASZMB010000000
VERSION     DASZMB010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2882946"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(297..488)
                     /locus_tag="VG272_06320"
     CDS             complement(297..488)
                     /locus_tag="VG272_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13331.1"
                     /translation="MTSVFGNLTHFGALVFFAAIVSVTFAFLSKRTNVERLKYSLLAF
                     LAFLVVAIGIGWLMYPFSQ"
     gene            complement(485..892)
                     /locus_tag="VG272_06325"
     CDS             complement(485..892)
                     /locus_tag="VG272_06325"
                     /inference="COORDINATES: protein motif:HMM:NF042755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6677 family protein"
                     /protein_id="HWF13332.1"
                     /translation="MDESVIMETGKGSQAMGIAAGVAGWIIPGLGHLILRRWTKAAIY
                     FLCVGALACAGLAMRGGVFGSGAEDLFDRLGFFADLGSGIFYFLAHQFQPAGPDVAHA
                     TGDYGTRLFAAAGMLNLLTVLEAYDIGHGGETK"
     gene            complement(915..2084)
                     /locus_tag="VG272_06330"
     CDS             complement(915..2084)
                     /locus_tag="VG272_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HWF13333.1"
                     /translation="MPNIEAIQAALKEQGIDGWLFYDILHRDPIAYRVLGLDHALAKR
                     RWFYMIPAKGSPRKLVHRIEAATLDSLPGDKMLYAAAEELVKHLNKLVGRTKKVAMQY
                     SPNNSIPAISLVDAGTVELIRSLGCKVVSSADLIQQFEATWTEQQLESHRVAGRAVDS
                     ITQGAFAEAGRRISAGEEFSEYDLQQWIVAQFRARGVASDSPPIVAVGPHSGDPHYEP
                     SPQNSAIIRRNDFLLLDIWAKTIAPGSVYYDITWVGYLGDRIPEKFVKIFSVVREARD
                     AAVHFVQKNMSAGRPVEGWQVDRAARGIIHKAGYAKYFVHRTGHNIGQEVHGAGANMD
                     GLETRDIRRIIPHTCFSVEPGIYLPEFGVRSEVDVYVGEKSAEVTGPAQTEILKL"
     gene            complement(2127..2414)
                     /locus_tag="VG272_06335"
     CDS             complement(2127..2414)
                     /locus_tag="VG272_06335"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HWF13334.1"
                     /translation="MSLDILEKNGSVSFAVRVQPRASCNEFAGVYQNGLKIRLAAPPV
                     DDRANEALRKFLASRLNVPMAAVRIAAGERSRTKRIEITGVTAATIRALAI"
     gene            complement(2411..3166)
                     /locus_tag="VG272_06340"
     CDS             complement(2411..3166)
                     /locus_tag="VG272_06340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HWF13335.1"
                     /translation="MGSASQEILKNFTEVHQRIDAAASRAGRRAEEITLIAVTKTHPV
                     SKILDLYSAGARHFGENRVQERESKLAGTAQMDATWHMIGHLQKNKVARVPGMFQSVD
                     SVDSLALAEKVDRAAGECLKIKNEWGDLQQNAFKNRLRVLIEIKLDPEPAKSGASPEE
                     LPRLVESILRMPHLNLQGLMSVPPYTDTPNDARPYFRRLRELRDQLRKEIGNQILPVL
                     SMGMSHDFEVAIEEGATEVRVGTALFGKREYGE"
     gene            3555..4244
                     /locus_tag="VG272_06345"
     CDS             3555..4244
                     /locus_tag="VG272_06345"
                     /inference="COORDINATES: protein motif:HMM:NF014299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13336.1"
                     /translation="MATRGKIIALEGIDGSGKGTQLDLLAGELKARKLPTYRISFPRY
                     GSFYGKLVGRYLNGEFGTLEAVDPHLSALLYAGDRLEAKPEIEAALAVGTIVLADRYI
                     GSNLAHQTARVAPEMQEEFIRWLKHLEYVLYGLPAEDLVIYLRVPFEEAHRLVGLKAT
                     RDYTRLKRDIQEADIKHLEQTGIIYDRLASESNWVRVDCENESSRMLRSPEEIHRLVM
                     QWVESQILERW"
     gene            4285..5415
                     /locus_tag="VG272_06350"
     CDS             4285..5415
                     /locus_tag="VG272_06350"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13337.1"
                     /translation="MGFSEFIGNQRVVTALRGMLATDRVAHAMLFSGPRGVGKFTLAR
                     MFAQAANCERLQDDFCGECEHCKHIALLANTAPLITQGLTQRGERPDAATVERVPLLL
                     ETHPDVWAIVPDPVRLKDPVTRPMLHMGQLRAVQRAAYFKPRGRRRVFIIDGAETMRW
                     DLSNIFLKILEEPPESSTLILLAPSPYQLLGTIRSRCLQFSFSPLAMPEVEAILSSHT
                     KMTPEERRLAAQYAEGCPGLAMGMDLDAVARLQRDALTILERSVELRGVSDLFASTAA
                     LAKSQEIAFENVLEVFYSLLTDLLDLSSTSTMKVLRNPALRRELETLSKKIDVAWINK
                     AVEGFDVLHARTRRNVNRSLGLDAVALSLTRPAPGIKKSFSR"
     gene            5529..5876
                     /locus_tag="VG272_06355"
     CDS             5529..5876
                     /locus_tag="VG272_06355"
                     /inference="COORDINATES: protein motif:HMM:NF028518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HWF13338.1"
                     /translation="MNRKLFRPTLTEMKEPHPSSARAHSSTNGPQPHKKPAPPEQTHA
                     ENFYWIKQMQGRTPVVIVMTDGENLQGVVEWYDKDCIKLTRTGSPNLLIYKQFIKYIY
                     KDGPDAALGGANG"
BASE COUNT         1401 a         1592 c         1674 g         1374 t
ORIGIN      
        1 ccggtgctac acggcaaatc ggcgggcggt gggcgtttat tcagaagatc acggagtcat
       61 tcaaatcgag agccttattt agagcagcaa cgttagcgct cggccgacca tgcaccgcta
      121 ggacgctcgc cgtgctattc attgcttggt tgcactgaat ggaaatgcct tcttgtgttt
      181 cgcaggtctt ggcacgttgg gctcaacaag acattagaat ctggcaagaa taatcacaga
      241 tagccatggc actgagatat cagtctttgc tgccgttccg gatgaccggc gacgtcctat
      301 tgcgaaaaag ggtacatcaa ccatccgatc ccgatggcga caaccaggaa tgcgagaaac
      361 gcgaggaggg aatacttcag gcgctcgacg ttcgtgcgtt tggaaaggaa tgcaaaggtg
      421 acggaaacaa tggcggcgaa aaatactaac gcgccaaaat gcgtgagatt tccgaacacg
      481 cttgtcactt cgtttctccg ccatgtccga tatcataagc ttccagaaca gtcagcaaat
      541 taagcatgcc tgcggcggcg aacaagcgag tgccgtagtc ccctgtggca tgggcgacgt
      601 cgggaccggc aggctgaaac tgatgggcca gaaaataaaa tattcccgaa cccagatcag
      661 caaaaaatcc taggcgatcg aataaatctt cggcgccgga gccaaaaact ccgccgcgca
      721 ttgcgagacc cgcgcaagcc agggcgccca cgcatagaaa atagattgcg gctttcgtcc
      781 agcggcgaag tatcaggtga ccgaggcccg gaatgatcca gccagcgacc cccgccgcga
      841 tgcccattgc ctggctgccc ttcccagttt ccattattac tgattcgtcc acgttcatgt
      901 cctcgcgttt agccttacag tttcagaatt tccgtctgcg caggtcctgt aacttccgcg
      961 cttttttcgc caacgtacac atccacttcg ctgcgcacac caaattcggg caaataaatt
     1021 cccggttcga ccgaaaaaca agtatggggg attatgcgac gaatgtcccg cgtttccaag
     1081 ccatccatat ttgcgccggc accgtgtact tcttgtccaa tgttgtggcc cgtgcgatgg
     1141 acaaagtatt ttgcgtaccc tgctttgtga ataattcccc gtgcggcacg atcgacctgc
     1201 cagccttcga ccggccgacc cgcagacata tttttttgaa cgaaatgtac ggcggcatca
     1261 cgcgcttctc gaacaacgga aaaaatcttg acgaacttct ccggaattct gtcaccaaga
     1321 tacccgaccc aggtaatgtc atagtaaacg ctgcctgggg ctatagtttt tgcccaaatg
     1381 tccagaagca gaaaatcgtt tcggcggatg atcgcggaat tttgcggcga aggttcgtaa
     1441 tgaggatcac cagaatgcgg tccaacggcg acaatcgggg gagaatcgga agccacgccg
     1501 cgcgcgcgga attgcgcgac gatccactgc tggaggtcgt attcggaaaa ttcttcgccc
     1561 gcagaaatcc ttcgcccagc ttcggcaaag gcgccttgcg tgatcgagtc gacggcgcgg
     1621 ccggccacac gatgcgattc cagctgctgt tcggtccatg tggcttcaaa ttgctgaatc
     1681 agatccgccg aactgacaac cttgcacccg agggagcgga tcagttccac agtgcctgcg
     1741 tctactaggg aaatagctgg gattgagtta tttggcgagt actgcatggc cactttttta
     1801 gtgcggccga ccagtttgtt tagatgtttc accaactcct ctgctgcggc atagagcatt
     1861 ttatcgccag gcagcgaatc gagcgtcgcc gcttctatcc ggtgcaccag ttttcgaggc
     1921 gagccttttg cgggaatcat gtaaaaccag cgacgtttgg ctaacgcgtg atccagaccc
     1981 agaacgcgat aggcgatagg atcgcgatga agaatatcgt agaagagcca tccgtcgatt
     2041 ccctgttcct tgagcgcggc ttggatggct tcgatattgg gcataaaaat tcggcggatc
     2101 gcttccgttg tgggaactta gccgttttag atcgcgaggg cgcgaatcgt tgcggcggtg
     2161 actccggtga tttctatgcg cttcgtgcga ctgcgctcgc cagccgcgat tctaacagcc
     2221 gccatgggca cattcaaacg cgaggccaga aattttcgta aggcttcgtt cgcgcgatcg
     2281 tcgaccggcg gcgcggccag ccggattttg aggccgttct gatatacgcc ggcgaattca
     2341 ttgcaacttg cccgcggttg cacgcgaact gcgaagctga ccgatccgtt tttctcaaga
     2401 atgtccaggc tcattcgccg tactctcttt tgccaaaaag cgcagtgccc acgcggactt
     2461 ccgtggcgcc ctcttcaata gcaacttcaa aatcatgcga catgcccatg gagaggaccg
     2521 gcagaatctg gttgccgatt tcctttcgga gctgatcgcg aagttcgcgt aagcgtcgga
     2581 aatacggcct cgcgtcgttc ggagtatcgg tgtacggcgg cactgacatt aaaccctgca
     2641 aatttaaatg cggcatgcgc aaaatggatt cgactagccg cggcagttct tcaggcgacg
     2701 cgccggattt ggcgggttcg gggtcgagct tgatttcaat caatacgcgg aggcgatttt
     2761 taaatgcgtt ctgttgtaaa tcgccccact catttttaat ttttaaacat tctccggcgg
     2821 cgcgatcaac cttctcggcc aacgctaggc tatcgacaga gtccacgctc tggaacatgc
     2881 cggggacccg cgcaacttta tttttttgaa gatgcccgat catgtgccaa gtcgcgtcca
     2941 tctgcgcggt gccggcgagt ttcgattcgc gttcctgtac gcggttttct ccgaaatggc
     3001 gcgccccggc gctgtacagg tctaaaattt ttgaaacggg gtgagttttg gtgacggcga
     3061 ttaacgtgat ttcttcagct cgacgaccgg cgcgggacgc ggcagcgtcg atgcgttgat
     3121 ggacttcggt aaagttcttc agaatttctt gtgaagcgga acccatgcag cgaattctag
     3181 catggtcgtc ttgagcacac tttgtcgcgg actaaatcac gcgtaagagc ctggaccacg
     3241 ccctcacatc gaagcctcag cagttgcgca gggtgcggaa aaaactgtca ttccgaagct
     3301 tgtgattcgc cgagaaattt ccgcttcatt ttggactgaa catcgaagag aaatttatcg
     3361 atatgtttag attaacgatt aatagtcgag tagcacaggc acgtttgcct gtgtgcattt
     3421 tgcaagaccc gcacaggcaa gaatgcctgt gcgactcagt cgatcagtcg aacggaatgg
     3481 agatgtgggt aaggtcgtga accgcaggct tccttgcgag gactgtggca tcacgctaga
     3541 ctgacacatc gaatatggcg actcgcggaa aaataattgc gctcgaagga atcgacggat
     3601 cgggtaaagg cactcagctg gatttgctgg ccggcgagct caaggcgcgg aaacttccta
     3661 cctatcgcat cagctttccc cgctacggat ctttttacgg gaagctcgtg ggccgttatc
     3721 tgaacggaga attcgggacg ctggaagcgg tcgacccgca tttatcggcg ctgctttatg
     3781 ctggagatcg actggaagca aagccggaga ttgaggctgc gctcgcagta ggaacaatcg
     3841 tgcttgcgga ccgctacatt ggctccaatt tggcacatca gacggcacga gtcgctcccg
     3901 aaatgcagga ggagttcata cgctggctga agcatttgga atatgtgctg tacggtttgc
     3961 ccgcggaaga tttagtaatt tacttgcgcg tcccgtttga agaggcacac cgattggtgg
     4021 gattgaaggc tacgcgcgat tacaccaggt tgaaacgcga cattcaagag gctgacatca
     4081 agcacttgga gcaaaccggg ataatctatg accggctggc gagcgagtcg aattgggttc
     4141 gcgtggactg tgagaacgaa agctcgcgta tgttgcgctc gcccgaagaa atacaccggt
     4201 tggtcatgca atgggtggaa tcacaaattt tggaacgatg gtagatcgat acgaatagat
     4261 tgagtaggga aaggcagata ggtcgtgggc ttttcagaat tcatcggcaa tcaaagggtg
     4321 gtgacagcgc tgcgcggcat gctggcgacg gaccgcgtgg cgcatgcgat gcttttttca
     4381 ggtccgaggg gcgttggaaa attcacgctc gcgcgcatgt tcgcgcaggc ggccaattgc
     4441 gagcgcctcc aggacgattt ttgcggtgaa tgcgagcact gcaaacatat cgcgttgctc
     4501 gcgaatacag ctccgctcat cacccaaggc ctcacgcagc gcggcgagcg gcctgacgcc
     4561 gcaacggtag aacgcgtccc gctgttatta gaaactcatc ccgatgtttg ggcgattgtg
     4621 ccggacccag tccggttgaa ggatccggtc acgcgaccga tgctgcacat gggacaactc
     4681 cgcgccgtgc agcgtgcggc gtacttcaag ccgcggggac ggcggcgggt tttcattatc
     4741 gatggcgcgg aaaccatgcg ctgggacctg tccaatattt ttctgaaaat tctcgaagag
     4801 cctccggaat cctccacgct gattctgctc gcgccaagcc cgtaccaact tttgggcacg
     4861 atccgctcgc gctgcctgca attttctttt tcgccgctgg cgatgccgga agtggaagca
     4921 attttgtcca gtcatacgaa aatgacgcca gaagagcgca ggctcgccgc gcaatatgcc
     4981 gaaggctgcc cgggcctcgc gatgggaatg gacctggacg ctgtcgctag gttgcaacgc
     5041 gacgcgctga cgattctcga gcgctccgtt gaattgcgcg gcgtttcgga tttgtttgca
     5101 agcactgcgg cgctggcgaa aagccaggag atcgcgtttg aaaatgtgct tgaagtgttc
     5161 tatagtctcc tcacggacct gctagacctg tccagcactt ccacgatgaa ggttttacgt
     5221 aacccagcat tacgccggga actcgaaaca ctcagcaaga agatagatgt ggcgtggatc
     5281 aataaggccg tcgaagggtt tgacgtactt cacgcccgga cacgaaggaa cgtaaaccgg
     5341 tcactgggat tggatgcggt agccttatct ctgacccgcc ccgcgccggg tataaaaaaa
     5401 tcattttccc ggtgatagcg accgtaccct tacccggtgg tcctgcatct tagctggtga
     5461 gtagtctcac ctagtcagtg gaattatata aattgttatt gcaattataa attcaaggag
     5521 ttttaaccgt gaatcgcaag ctatttcgtc ctacacttac ggaaatgaaa gagccacacc
     5581 ccagctccgc acgtgctcat tcgtccacca acgggccgca acctcacaag aagccggctc
     5641 ctccggaaca gacgcacgcc gagaactttt actggatcaa gcagatgcag ggccgcacgc
     5701 cggtggtaat cgtaatgacg gacggcgaaa atttgcaggg cgtggtcgag tggtatgaca
     5761 aggactgtat taagctgacg cggaccggaa gcccaaacct gctgatttat aagcaattca
     5821 taaagtatat ctacaaagat ggtccggatg cggcgctcgg cggggccaac ggctaattaa
     5881 tttcttctat gtagcgctcg tcttcaggcg aacatgcggg taattcccaa atgcccgcct
     5941 gaaggcgggc gctacaaaaa cctaccgatt ttgatctctt acagagacgc atgcgcctta
     6001 tttcttcggc gtcgacactg agaatgtcat cagagcttta c
//
LOCUS       DASZMB010000136         3879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_423002, whole genome shotgun sequence.
ACCESSION   DASZMB010000136 DASZMB010000000
VERSION     DASZMB010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423002"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..689)
                     /locus_tag="VG272_06360"
     CDS             complement(21..689)
                     /locus_tag="VG272_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF13339.1"
                     /translation="MAKTTKAQIRVLMADDHAIFREGLRKLLDGDDEITIVGEASNGN
                     DCIKMLAKLKPDILLLDLRMPDKDGLAVLEEVNFDQLPTRVVVLTAAEDDRDVVRAMR
                     LGARGVVLKQSATDLLVRSIHKVHSGEIWLDNHMTAEVMKAFSKSSDGGPRREKPLLS
                     DREKEIVQLVAQGYRNKEIGEKLFISEQTVKNHLHNIFDKLGVSDRLELALYAIHHRL
                     IDQA"
     gene            complement(691..2361)
                     /locus_tag="VG272_06365"
     CDS             complement(691..2361)
                     /locus_tag="VG272_06365"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWF13340.1"
                     /translation="MQPHIAERRHLERQIAYARPIFMVLALVDLLDRPPAERGLHAVW
                     FVTVYLIVSLALAVVQNLRWINEFVVPVQVDLAALAVFLVLTRSVVAFWFVYLFVALA
                     AGIRWGARRSVMLAGIVTLALLIRTAMNGPLAWEQMLSWIALATGTFGAGAGMSFLGS
                     RQRRHATEQEFLALVTGKIEVQRGMAESLRLVLGELARAFNCEEAILVFRDTDIERIF
                     VWKYRDGDETRLTPENLPVNRSDGFLLDQPDASVCWNELEGAGEGFGWSRKDGRRLKD
                     LPRLPGPMRQELGWRSVLGVTLDLGGQPVGRLLLGNARARFIPQDLHWMERIVRHLGQ
                     PLQNLFLLRHIRAHAIEGERSRISREIHDGILQTLLSVDIQLDVLRRKVPTDPEQAVG
                     ALSSLQQTVRNETDELRRMVTDMRPLRVQSADLVDLMRGFGERFRNESGLALDLLIDA
                     AELQIPDRICRDLFQIYRESLHNVKKHAHASHVVVKLWQNEGEVVLVVDDNGQGFSFA
                     GRFTGDELDRLRLGPISIKERTRSVGGVLTVESTPGHGARLSIQVPLG"
     gene            2522..3466
                     /locus_tag="VG272_06370"
     CDS             2522..3466
                     /locus_tag="VG272_06370"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF13341.1"
                     /translation="MLRSKTIRGAGRLEHRYTRNEVERMTGVSRRDLEYWTRLRLLLP
                     RARWGERFYNFSDLVALETIKRLAAQKVPASRIKRAITTLQTELGSTKTPLSMLRISS
                     NGKQVVVHPPLEARPFEPLTGQFVLDFETSVIAKKIHAMASRSAEEWFEFGMLCDSDS
                     GSVSEAARAYQKAVEAAPEWVEARINLGTAMYQLGRMEEAYEHFLMAVGFEPDNALAE
                     FNLGCVLEQLAKTDEAIIHLSRAVELAPALADGHLNLALAYERTGRVDGALRHLSLYI
                     RYEPNGPWAEFARSRIEKYRPPSPTASAGKLTTFRKLN"
     gene            3656..>3879
                     /locus_tag="VG272_06375"
     CDS             3656..>3879
                     /locus_tag="VG272_06375"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWF13342.1"
                     /translation="MIIGDQLRSIREEKKLSQGEIEKRTGVLRCYISRVENGHTVPAV
                     ETLEKFARALEVPLYQLFYDSDKPPALPNIL"
BASE COUNT          907 a         1057 c         1075 g          840 t
ORIGIN      
        1 tgaaaaattg tgcggaaaaa tcaggcctgg tcgatgaggc ggtggtggat ggcgtagagg
       61 gccaattcca ggcgatcgga aacgcctagc ttatcgaaga tattgtgcag gtggttcttc
      121 acagtctgct cgctaatgaa aagtttttcg ccgatttcct tgttgcggta accctgcgca
      181 acgagttgca cgatttcttt ttcgcggtcg ctgagcagcg gtttttcgcg ccgcgggccg
      241 ccgtcggacg acttggaaaa cgctttcatg acttcggccg tcatgtgatt gtccagccag
      301 atttcgccgc tgtgcacttt gtgaatgctg cgcaccagca aatctgtggc ggattgcttc
      361 agcactacgc cgcgcgcgcc gaggcgcatt gcgcgcacca cgtcgcgatc atcttcagca
      421 gcggtgagaa ccacgacgcg agtcggcagc tgatcgaagt tcacttcttc caaaaccgcg
      481 agtccatcct tgtccggcat gcgcagatcc agcagcagaa tatcgggctt cagcttggcc
      541 agcattttga tgcaatcgtt gccgttggag gcttcgccaa caatggtgat ttcatcgtcg
      601 ccgtcgagca atttgcgcag accttcacga aagatggcgt gatcatccgc catcaggaca
      661 cgtatttgag ctttggttgt tttggccata tcagccgaga ggaacctgaa tagacaaacg
      721 agcgccatga ccgggcgtgg actctacggt cagaaccccg cccacactgc gcgttcgctc
      781 cttaatggaa atcggcccca accgaaggcg gtctaattca tctccggtaa agcgaccggc
      841 aaaactgaaa ccttgtccgt tatcgtctac aacaagaaca acttcgcctt cattctgcca
      901 tagttttacc acgacgtggg aagcatgcgc atgttttttc acattgtgta acgattctct
      961 atatatctga aagaggtcac ggcagatgcg gtccgggatc tgcagttccg ccgcatctat
     1021 aagtaaatca agggctaagc cggattcgtt acgaaacctc tctccgaagc ctcgcatcag
     1081 atccaccaaa tccgcgcttt gaacgcgcag cgggcgcata tccgtgacca tgcggcgcaa
     1141 ctcatccgtt tcatttcgaa ccgtttgctg caaggaagat aatgcaccga ccgcttgctc
     1201 aggatcggtt ggcaccttac gacgtaaaac gtcaagctgg atatccacgc tcagcagggt
     1261 ctgcaagatg ccgtcgtgga tttcgcgcga gatgcggctg cgctctccct caattgcatg
     1321 cgcgcgaatg tggcgaagca ggaacaaatt ctgcaacggt tggccgaggt gacggacaat
     1381 tcgctccatc caatgcaagt cctgcgggat gaaacgggcg cgcgcatttc ctagcaggag
     1441 acgtccaacc ggctggccgc caaggtccag agtaacaccc agaacagaac gccagcccaa
     1501 ttcctggcgc atcgggcctg gcaaacgggg caaatctttg aggcgccggc catccttgcg
     1561 gctccagccg aagccttccc ctgctccttc caattcgttc cagcatacgc tggcgtccgg
     1621 ctggtccagg aggaagccgt cgctgcgatt tacgggcaga ttttcgggag taaggcgagt
     1681 ctcgtcgcca tcgcggtatt tccaaacaaa gatacgctcg atatccgtat cgcggaaaac
     1741 gaggatcgct tcttcacagt taaacgcacg cgcaagttcg ccaagtacca aacgcaggga
     1801 ttccgccatg ccgcgctgta cttcgatttt tccggttacc aatgccagga attcttgctc
     1861 tgttgcgtgg cggcgctggc ggctgcccag gaacgacatg ccagcgccgg cgccaaatgt
     1921 tcccgtggcc aacgcaatcc aggagagcat ttgctcccat gcgagcggcc cgttcatcgc
     1981 cgtccgaatc agcagcgcga gcgtcacgat tccggcgagc attacggagc gccgagctcc
     2041 ccaacggatt ccggcagcaa gcgcgacaaa aaggtagacg aaccagaagg ccacgaccga
     2101 ccgggtaaga accagaaaca cggccagcgc ggccaaatct acttgaacag ggacgacaaa
     2161 ctcgttaatc caacggagat tctgtacgac ggcgagcgcc aaggagacaa tcagataaac
     2221 ggtgacgaac cataccgcat gtagcccacg ctcggcaggc gggcggtcca gcagatcgac
     2281 cagagccagc accatgaaaa tgggcctggc gtaagcgatc tggcgctcca ggtggcgtcg
     2341 ttcggcgatg tgaggctgca taatgggaag aggtatatcg cggacaggat ttagtctaac
     2401 aagactggca cggcgtgcaa gacgcgcgtt cgcatcctgc gcgtagctgc aaacagtttt
     2461 ccgcggtttt ccaccgttat ttctgaaaaa attccctcaa tttgggggct tccgggcgat
     2521 aatgttgcgc agtaaaacca tccgtggagc tggccgcttg gaacaccgct acacccgcaa
     2581 tgaagtggaa cgcatgacgg gagtgtctcg ccgcgatttg gagtattgga cgcggctgcg
     2641 cctattgctg ccacgagcgc gctggggcga acggttttac aatttttcag acctggtggc
     2701 gctggaaacc atcaagcgac tggccgcgca aaaagttcct gcaagccgca tcaaacgcgc
     2761 gattacaacc ctgcagacgg aattagggtc gacgaaaacg ccgctctcca tgttgcgcat
     2821 ttccagcaac ggtaagcaag tggtggtgca tccgcctttg gaggcgcgtc catttgagcc
     2881 gctgacaggg caatttgttt tagacttcga gacatccgtt atcgccaaga agattcacgc
     2941 catggcgtcg cgctcagcag aagaatggtt tgaatttgga atgttgtgcg attccgactc
     3001 cggctcagtg agcgaagccg cgcgcgcgta ccagaaagcc gtggaagcgg cacccgaatg
     3061 ggtggaagcg cgcatcaacc tcggaacagc aatgtatcag ctcgggcgca tggaagaagc
     3121 gtacgagcat tttttaatgg ccgtgggatt tgaaccggac aacgcgctgg cggaattcaa
     3181 tctcggctgt gtgctggaac aattggcaaa aacggatgaa gcaattatcc atctttctcg
     3241 cgcggtggaa ctggctccgg ccctggcaga cggccacttg aatcttgcgt tggcgtatga
     3301 gaggaccgga cgggtggacg gtgcgttacg ccacttgtct ttgtacattc gttatgaacc
     3361 aaatgggcca tgggcagagt tcgctcgctc tagaatagaa aagtataggc cgccgagccc
     3421 gacagcttct gcggggaaat tgaccacttt tcgtaaattg aattagagga tgcctcttcg
     3481 ccgtgtcagg ctttacccag cttttggcac atttgtatgc cgctaagacc tgagcgcgca
     3541 tcgaaggaat ttgatatagt atttctatag ggtactgttc gtcttgaagc actaatccgt
     3601 agaacttttt tatgtggttt gatttttttc agcaaaattc gaaaggacaa acataatgat
     3661 tatcggggac caattgcggt cgatccgcga ggaaaaaaag ctctcgcaag gtgaaattga
     3721 aaaacgtacg ggcgtgcttc ggtgctacat ctcccgcgtg gaaaacggac atacggttcc
     3781 cgcggtggaa acactcgaaa agttcgcacg cgctttggaa gtgcctcttt atcagctttt
     3841 ttatgacagc gacaaaccgc ccgcgctgcc caacatcct
//
LOCUS       DASZMB010000137        10396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4267875, whole genome shotgun sequence.
ACCESSION   DASZMB010000137 DASZMB010000000
VERSION     DASZMB010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 10396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10396
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4267875"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..2004)
                     /locus_tag="VG272_06380"
     CDS             complement(130..2004)
                     /locus_tag="VG272_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWF13343.1"
                     /translation="MTTLTKAGRYDLREELGRGAMGVVFHGFDPVIGRAVAVKTLRLS
                     EAGTGLSREELVGRFQTEARAAGLLAHPNIVVIFDAGEEDGLFYITMEFVEGRSLQSV
                     IDARQPFPVPRVMKLMEQVCSALDFAHQHNVVHRDIKPANLMLTADDVVKITDFGTAK
                     ILQFGTAQTAHVMGTPSYMSPEQVKGKPVDGRSDIFSLGVILYELMTGEKPFPGQNIT
                     TVIYKIINEEPISPRSLDSSIHPGLSAVITRALAKEPADRFQSCHELMSALKNYHELS
                     GTESTGRMAPVSSKSPVGLNRTPIQPLRPTAITPPLRSAAIQPISKSNETEIPALPFS
                     PIEMEKESPKNRGGWLLTLILLGIIGYAGYRVYPPALDLWQRTHEEIEKPAVPPQTET
                     APTAPASDNTGTKSDALFSDAPLTSAPITSPAPADDPSPLNAMQPDATTTAPVKTPDI
                     AAPAALNTDKAIAQPKAAPAVAPSAAHLFELKLRSELSDSALNHKLNTRATANALTLS
                     GSLSIAEHRELMTHLHTVPPGVRVIDDVDLTGDAKAAPTAASAGWIWVRSSPPAARIL
                     VDGRETGMRTPSKLELQSGEHVVQLVREGFGTAHRKIAVASGQTLQFTETLPIEQNPQ
                     "
     gene            2379..4307
                     /locus_tag="VG272_06385"
     CDS             2379..4307
                     /locus_tag="VG272_06385"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWF13344.1"
                     /translation="MTKSPKPVLDRRNFLKGAAAGAAALAAVPASVAAQPEAKRTVPP
                     PMSPAAETEVPTGAEVLTADRTGSDYMVDVIKSLGFEYVCANPGSSFRGIHESIINYG
                     GNKAPEFITCCHEESSIAMAHGYYKVEGKPLAVLAHGTVGVQHAAMAIYNAYCDRVPI
                     YLILGNIIDETKRAPGVEWFHSVQDAAAMVRDFTKWDDLPISLEHFGESAVRAYKVAM
                     TPPTLPVVIVADGELQENPVPTDAKIRIPKLTNEMPPVGDSGSVNETARLLVAAENPV
                     IIADRTARTPAGLACLVDLAEALQCAVIDQAGRMNFPTRHPLNQSDRSRAVIAEADVI
                     LGLDVADLYGITHSFRDQIERTSKSGTKPTAKVITISSDDLFIKANYQDFQRYNEADI
                     AMAADSQATLPSLTEAVKRLTTDDRKRAFQDRGVRLKASHQQALERSRDAAAFAWDAS
                     PISTARMCMELWGQIQDEDWSLVGATTFVSKWPQRLWNFDKHYQYIGGAGGYGIGYNA
                     PAATGAALANRKHGRLTVNIQCDGDLMFAPGILWTAAHHKIPVLHVMHNNRAYHQEVM
                     QVQIMADRHSRGIANCGTGTTITGPDISYAKLAESMGMYGEGPISDPKDLGPALKRAI
                     EVVKRGEPALVDVVTQPR"
     gene            4343..4720
                     /locus_tag="VG272_06390"
     CDS             4343..4720
                     /locus_tag="VG272_06390"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWF13345.1"
                     /translation="MKRYCGLFIFMLAATLFAIPTARAQQSSDAPPAGNAQNGKKLFN
                     TVGCYECHGRQGQGAAQTGAARIGPPMLSFDGFLGLVRKPINIMPPYSAKAISDQDLA
                     DIYAYLKSIPMPPKGKDIPLLNK"
     gene            4803..5243
                     /locus_tag="VG272_06395"
     CDS             4803..5243
                     /locus_tag="VG272_06395"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWF13346.1"
                     /translation="MLRTRNDGVLTLIRIVAGGVMLPHGMQKMFGWFGGAGFAGTIHG
                     FGQRGFPAPLVFLVIMAEFFGSIGLIVGFLSRIAALGIAIDMTVAAITVTSKFGMFIN
                     WAGKQKGEGMEYQLLMIGMTLAIVIAGGGALSVDRAVAGGNSRS"
     gene            5360..6265
                     /locus_tag="VG272_06400"
     CDS             5360..6265
                     /locus_tag="VG272_06400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HWF13347.1"
                     /translation="MTTDLSRRSIDFRSVWNLGGLTPWKLTRRVFSDAIADDLAERAS
                     GLAFDLLLAFFPLLVFLLAIFGLFASRSIELRIDLLSYFADFLPPMAFQLLSGTIDEL
                     AASASRGKLTIGILGVLWFASGGVSSMISGLHWAYRVGDDRSWLTVRAVAIGLTLIIA
                     VLIVSALSLVLVGGYLMTWAVQELHPTSVMVSIWKVLQWPIAWLFVVFSYVLINSYGP
                     NADRRRWYWITPGSAFGALLWLAASVGFRIYLNFYNNYPAIYGSVGAVIILVVWLYVA
                     GLTFLIGGEIDANIERAIDETQGKS"
     gene            complement(6366..7361)
                     /locus_tag="VG272_06405"
     CDS             complement(6366..7361)
                     /locus_tag="VG272_06405"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWF13348.1"
                     /translation="MRSWRTRARELRGVVRGVMSTRHPLLVHIIPTRRCNLSCTYCNE
                     YDDFSKPVDTAEMLRRIDRLGELGTSVVTISGGEPLLHPDLDVIIARMRKNGILSGMI
                     TNGYLLTADRIERLNRAGLEYLQISIDNAMPDEVSMKSLKVLDQKLKLLAEHAVFQVN
                     INSVLGSGVKNPEDALMVARRAQELGFTSTVGILHDGQGQLKPLSGRAIEIFEEIMAM
                     GKRGFSRINGFQHNIAKGKTNDWRCRSGSRYLYICEDGLVHWCSQQRGYPGIPLEKYS
                     FEDLRREYFTKKHCAPLCTVSCVQQIAVVDNWRGPQTLKPVATPPSSPESLVQLR"
     gene            7549..8127
                     /locus_tag="VG272_06410"
     CDS             7549..8127
                     /locus_tag="VG272_06410"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HWF13349.1"
                     /translation="MSKGRLEAFSDGVIAVIITIMVLELQAPHGATLSDLKPKIPAFL
                     SYVLSFIFIGIYWNNHHHLLHAITRVNGRVLWANLHLLFWLSLIPFATDWMGVNQWAT
                     VPVAVYGCLLFMAAAAFTILAYELIALEGKDSTLARALGRDYKGKTSLLLYALAIPLA
                     YLNPRISTAIYVLVAFIWLIPDRRIEKVVAER"
     gene            8345..9673
                     /locus_tag="VG272_06415"
     CDS             8345..9673
                     /locus_tag="VG272_06415"
                     /inference="COORDINATES: protein motif:HMM:NF024765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HWF13350.1"
                     /translation="MKLKWISCIVCFLGIIFCHFIPLLVAAQNQSTQTTTAQDPAPQE
                     SAPRELWSFLKEGKISFSTRWRFESFERDGAPFTGIAEAPTLRIALGYESPEYHGFTA
                     FAQGESVIVTGAANYSIPLRPSQNHPNLPSILDPKSLEMNQAYVKWERHISDKNFSLT
                     AGRQELTLNDGRFISYSNWRQVHGSFDAVRLEAELPRNFSVTYAFINRFYREDGYDAL
                     DGEEPMHTDMVNLKWNKADKLNASVYALLLNFRGPAQFATSTQTYGARVTGPYQFTPD
                     WSLLYVAEFAKQKNYGTNPNKEDENYYLGEIGPGWRDFSVKAGYAFLGGRSATDLLTT
                     PLAPPRNGWTDLFFTNPSITGGHGLEAAYVNASGPIRFLDGAIATFIYYDYHSDYPHI
                     HYGSELDSSLAYKIKRISNRWEIGWRFGRYWSDHLYSNSLRTSVYTIFTL"
     gene            complement(9776..>10396)
                     /locus_tag="VG272_06420"
     CDS             complement(9776..>10396)
                     /locus_tag="VG272_06420"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:NF018201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0061798 - GTP 3',8'-cyclase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HWF13351.1"
                     /translation="RDMHAEVLAGIAAARRLGFPKLKLDTVVLRGVNEDELANLIEYG
                     RGVEAEVRFIEYMDVGGATEWSMEKVFTRAEMLDVLSKRYGTITALNENSSAPAERFQ
                     LPDGTVFGIIASTTTPFCKTCDRSRLTADGLWYLCLYAQAGIDLRKILREGATKEELA
                     ARISSIWQARTDRGAELRAATGQRGVLIGIEQLRKDPHLEMHTRGG"
BASE COUNT         2319 a         2739 c         2819 g         2519 t
ORIGIN      
        1 caaatatgcc aatgaattaa cctacgaaaa atagtgggat agaatcgtta caaccgagga
       61 agattgagtt ccacttagtt ggctttggaa atgccctttt ggaagtcttg aaacgaaagc
      121 tcctggacgc tattggggat tctgttcaat tgggagtgtt tccgtaaatt gcaatgtttg
      181 acccgatgca accgctattt tcctgtgggc ggttccaaat ccctcgcgaa ccaattgaac
      241 gacatgctca ccggattgca attccaattt tgagggagtt cgcattcctg tctcccttcc
      301 gtccaccaga attctggctg cgggagggga agaccgaacc caaatccaac ctgccgaagc
      361 tgccgtgggc gcagccttgg catcgccggt caggtcaaca tcgtcgataa cgcgaactcc
      421 cggaggcacg gtatgaagat gggtcatcaa ttcgcggtgt tcggcaatgc tcaacgagcc
      481 tgacagagtc agggcattcg cggtggcgcg cgtattcaat ttatggttta acgccgaatc
      541 ggacaattct gagcgcaatt tcaattcaaa cagatgtgcc gccgaagggg ccacagcggg
      601 tgcagctttt ggttgagcga tagccttatc agtgttcagg gcagcgggcg cggcgatatc
      661 gggagtcttc acgggcgccg ttgtcgtggc gtctggctgc atggcattca acggcgaagg
      721 atcatctgcc ggtgcgggag atgtaatggg cgcggaagtc agcggcgcat cgctaaatag
      781 agcgtctgat ttcgtgccgg tattatcaga ggcgggtgcc gttggcgcag tctccgtctg
      841 gggtggaacc gcgggcttct ctatctcttc atgagtccgc tgccacaaat ccagggcggg
      901 agggtagacg cgataacccg catacccaat aattccaagc aaaatgagag ttagcagcca
      961 tccgccgcga tttttgggag attccttctc catctcaatg ggcgagaacg gcagggcagg
     1021 aatttcagtt tcgttcgatt tcgaaattgg ctgaattgca gcagagcgca atgggggggt
     1081 aatcgcggtg ggacgtagtg gctggatcgg cgttcgattc aagcccacag gtgatttcga
     1141 actgacgggt gccatccgcc ctgtcgattc ggtcccggat aattcatggt aattcttcaa
     1201 agcgctcatt aactcatggc agctctgaaa gcggtccgca ggttctttcg ccaacgcacg
     1261 tgtaatcacc gcggaaaggc cgggatgaat ggacgaatcg agcgatctcg gtgagatggg
     1321 ttcttcattg ataattttat aaataacggt ggtgatgttt tgaccgggaa atggtttctc
     1381 tcccgtcatc agttcgtaga gaatcacgcc aagggaaaaa atgtcggacc ggccatcaac
     1441 cggcttgcct tttacttgct caggagacat gtaggaaggc gtgcccatca cgtgagccgt
     1501 ctgcgccgtt ccaaactgaa gaattttggc cgtgccgaag tcggtaattt ttacaacatc
     1561 atcggcggtc agcatcaggt tcgcgggttt aatgtcgcgg tgtactacgt tgtgctggtg
     1621 cgcgaaatct agggcggagc aaacctgctc catcagtttc atcacacggg gaacagggaa
     1681 tggttgccga gcgtcgatca ccgattgcag acttcgcccc tcgacaaact ccatcgtgat
     1741 gtagaacagc ccgtcttctt caccggcatc gaagattaca acaatgtttg gatgggcaag
     1801 cagcccggcg gcgcgtgctt cggtctggaa gcgcccaacc aattcttcgc gcgagagccc
     1861 cgtacccgcc tctgacaatc gcaaggtctt aacggccaca gctctgccaa tcactggatc
     1921 gaaaccgtga aacaccacgc ccattgcgcc gcggccaagt tcctcgcgga ggtcgtaacg
     1981 tcctgctttg gttagggtcg tcactttttt agacgcgctg gcgtagcgga ggctgctcca
     2041 tgacctagtc agctcaatct gacattaact tgcaggcgcg gtcttatcaa gatcactttc
     2101 tggtatgcgc tagcgccatg gctaaattgc catgggggga accgccagca cgccgcactc
     2161 cttcaccttc gaacataact gtaggtcaag ggagcgtcaa cgccgctgag gtacagtgtt
     2221 ccggaatagc aatggtgtag cccttttgtt agaaccgctc acggatgctc ggagtttcgg
     2281 tcgcaattca atcttgaaac ccactgcttg cggtatggca tctgtatcaa ttcctgtgat
     2341 ataaagttaa aactgctcaa atatcgaggc gcaaacctat gacgaaatca cctaaaccgg
     2401 tcctggaccg ccgtaatttc ctgaagggag ctgctgcagg ggctgcggcc ttagcggccg
     2461 tgcccgcatc tgttgcggcg caacctgaag cgaagcggac agttccaccg cccatgtctc
     2521 cagcggctga gactgaagtg ccaaccggcg ccgaagtctt gacggcggat cgcactggtt
     2581 ccgattacat ggtggatgtg atcaagtcgc ttggttttga gtacgtatgc gcgaacccag
     2641 ggtccagctt ccggggtatt catgagtcta ttattaacta tggaggcaac aaagcacccg
     2701 agtttattac ctgctgccat gaagagtctt ccatcgccat ggcccacggc tactacaagg
     2761 tggagggtaa gccgctggcg gtgctcgcgc acggaacggt tggcgtacag cacgccgcga
     2821 tggccattta caacgcctac tgcgatcgcg ttcccattta tttgattctc ggaaatatta
     2881 tcgacgagac aaagcgcgct cccggcgtcg aatggttcca cagcgtgcag gatgccgcgg
     2941 ccatggttcg cgattttacc aagtgggacg atcttcccat ttcactggag cattttggcg
     3001 aatccgcggt tcgtgcctat aaagtcgcga tgacgccgcc gaccttgcct gtcgttatcg
     3061 ttgcggacgg cgaattgcag gagaatcccg ttccaaccga cgcaaaaatt cgcataccca
     3121 aactgacgaa tgaaatgccg cccgtcgggg attcaggctc agtgaacgaa accgcgcgtc
     3181 tcctggttgc tgccgagaat cctgtcatca ttgctgatcg cacggcgcga acacctgctg
     3241 gtctcgcgtg tttggttgat ctcgcggaag ccttgcagtg cgcggtgatc gatcaggcgg
     3301 ggcgcatgaa ttttcccacg cgccatccgc tgaaccaatc tgacagatct cgcgccgtca
     3361 ttgccgaagc ggacgtaatt ctcggattgg atgttgctga cctgtacggt atcacgcact
     3421 cctttcgcga ccagatcgaa cgcacgtcga aatccggaac aaagccaacc gcgaaagtca
     3481 ttacgatcag ttcggacgat cttttcatca aggcgaacta ccaggatttc cagcgataca
     3541 acgaagctga tattgccatg gctgccgatt cccaagccac gctgccttcg ctgacggaag
     3601 cggtgaagcg cctgacgaca gatgatcgca agcgtgcatt tcaagaccgc ggtgtgcgcc
     3661 tgaaagcctc gcaccagcag gcgcttgagc gttctcgcga cgcggcggct tttgcctggg
     3721 acgcaagccc gatcagcacg gcacgcatgt gcatggagct ttggggacag attcaggacg
     3781 aggattggtc tctcgtcggc gccactacct ttgtcagcaa gtggccgcaa cgcttgtgga
     3841 attttgacaa gcactatcaa tacatcggcg gggcaggcgg gtacggtatt ggctacaacg
     3901 ccccggcagc cacgggagca gcgctggcga atcgtaaaca cggacggctg acggtcaaca
     3961 ttcagtgcga cggcgacctg atgtttgcac ccggtatttt atggaccgcc gcgcatcaca
     4021 aaattcccgt gttgcacgtc atgcataaca accgcgccta ccatcaggaa gtgatgcaag
     4081 tgcaaattat ggcggatcgc cacagccgcg gaatcgccaa ttgtggaacc ggaacgacca
     4141 ttaccggtcc ggatatttct tacgccaagc ttgccgaaag catgggcatg tacggcgaag
     4201 gcccgatctc ggatcccaag gacctcggcc ctgcactcaa acgcgccatc gaagtggtaa
     4261 aacgcggcga gccggccctg gtggacgtgg tcacgcaacc tcgataataa aaaatgatta
     4321 tttcgaaaag gagatttgcg tgatgaaacg gtactgtgga ctttttattt ttatgcttgc
     4381 cgcaacgctg tttgcaattc caacggcccg agcccagcag tcttccgatg ctcctcctgc
     4441 aggcaacgcg cagaacggaa aaaagctgtt taatacggtg ggatgttacg agtgtcacgg
     4501 gcggcaaggg cagggagcag cccagaccgg cgcggcgcgc attggcccac cgatgttatc
     4561 gttcgatggc ttcctgggct tagttcgaaa gcccataaat ataatgcctc catattccgc
     4621 caaagcgatt tcggatcagg atttggccga tatttatgcc tacctgaaat ctattccgat
     4681 gccgccgaag ggcaaggata ttcctcttct caataaataa acacactcag agatatctta
     4741 aatttcagct gatccgattg tttaagtgaa aggaacgccg ggaggcgatt acgtttagaa
     4801 aattgctgcg aaccaggaac gacggcgtgc tcacgttgat tcgcatcgtc gcgggtggcg
     4861 tgatgctccc tcatggcatg caaaaaatgt tcggatggtt cggcggcgcc ggttttgccg
     4921 gcacaatcca cggctttggc caaaggggat ttcctgctcc gctggtattt cttgtgatca
     4981 tggcggaatt ttttggttcc attggattga tcgtcggctt cctcagccgg attgcggccc
     5041 tcggcattgc cattgacatg acggttgccg caattaccgt gactagtaag ttcgggatgt
     5101 tcatcaattg ggccggaaag caaaaaggcg aggggatgga ataccagctt ttgatgattg
     5161 gaatgacgct ggctatcgtc atcgcaggcg gcggcgcgct ctcggtagac cgcgcagtgg
     5221 ccggaggaaa tagcaggagt taatttccgc attgccccct gtttgtttat aaatcgcaat
     5281 ttgcaatgag tgatcaccca gcccccggtt ttcgtcagca gaaacgcaac cgaaactctc
     5341 tcattcgagt tctgccgata tgaccactga ccttagccgt cgcagcattg atttcaggtc
     5401 ggtgtggaat ctgggcgggc tgactccctg gaaactcacc agacgtgtgt tcagcgacgc
     5461 gatagcggat gatttggccg agcgcgcttc cgggcttgct tttgatttgc ttctggcctt
     5521 tttcccacta ttagtttttt tgctcgcaat cttcggcttg tttgcttctc gaagcattga
     5581 gttacggatt gatttgctca gctacttcgc tgattttctt ccgcccatgg cttttcagct
     5641 tttgagcggc acgattgatg agcttgcggc aagtgcaagc aggggaaaac tgaccatcgg
     5701 catcctgggc gttttgtggt tcgcgtccgg aggagtttct tcgatgattt ccggcctgca
     5761 ttgggcgtat cgcgtgggag atgaccgttc gtggctaacg gtcagggccg tcgcgatcgg
     5821 actgacgctg attatcgccg ttttaattgt ttcagcttta tctctcgtac ttgtgggtgg
     5881 ctacctaatg acctgggcag tacaagaact tcacccgact tctgtcatgg ttagtatctg
     5941 gaaagttctg caatggccga ttgcctggtt gttcgtcgtt ttctcttatg tcctgattaa
     6001 ttcgtatggg ccaaacgctg atcgaaggcg gtggtattgg atcacgccgg gttccgcgtt
     6061 cggcgcgctt ttgtggctgg cggcgtccgt aggatttcgc atctacttga atttttataa
     6121 taattacccg gcaatttacg ggtcggtggg cgccgtgatt attctcgtcg tctggctcta
     6181 tgttgccgga ttaacattcc tgattggtgg ggagatcgac gccaatatag agcgcgccat
     6241 tgacgaaact caaggaaaat cgtagttggg aattctggaa gaaaaataaa aagaaataga
     6301 aacattaaaa taattttatc aaattaaata taggtcgcgg ttttcaccag ccacgaatca
     6361 ctcttctatc gcaattgaac gagtgactcc ggcgaagacg gcggcgttgc cacgggcttc
     6421 agcgtctgcg gcccgcgcca gttgtcgacc acggcgattt gctgcacaca cgaaacggtg
     6481 cagagcggcg cgcaatgttt tttcgtgaaa tattcgcggc gcaaatcttc aaatgaatat
     6541 ttttcgagag gaatgccggg atacccgcgc tgctgcgagc accagtgcac gagtccatcc
     6601 tcgcagatgt acagatagcg ggagccggag cggcagcgcc agtcatttgt cttgcccttg
     6661 gcgatgttgt gctgaaatcc gttaatgcga gaaaatccgc gcttgcccat ggccataatt
     6721 tcttcaaaaa tttcgatggc gcgcccgcta agcggtttca gttggccctg gccgtcgtgc
     6781 aaaattccca ccgtgctggt aaatccgagt tcctgcgccc ggcgcgccac catcagggca
     6841 tcttcgggat ttttcacgcc gctgcccaac acggaattga tgttcacctg aaacacggca
     6901 tgctcggcca gcaactttaa tttctgatcc agcaccttaa ggcttttcat cgacacttca
     6961 tcgggcatcg cgttatcgat gctgatttgc aggtattcga gccctgcgcg gttgagccgc
     7021 tcgatgcggt ccgcggttaa caggtaaccg ttggtaatca ttccggaaag aatgccgttc
     7081 ttgcgcatgc gcgcgatgat tacatcgagg tccggatgca gcagcggctc gcccccactg
     7141 atcgtgacaa ccgacgtgcc cagttcgccg aggcgatcga tgcgccgcag catttccgct
     7201 gtgtccacag gcttagaaaa atcgtcatat tcgttgcaat acgtgcacga cagattgcag
     7261 cgccgggtcg gaataatgtg cacgagcagc gggtgccgcg tggacatgac tccccgcacg
     7321 acgccgcgca gttcgcgcgc gcgcgtgcgc caggatcgca atcgcctccg caactttatg
     7381 ctaaatggca tcgctaaact atacagcgaa tcgcaaaagt tttgaactcg gtctgtaatc
     7441 tccagcaatc aatgcattac aggtgatggg tgtggagagt gacccggcgt ccgcttgtgc
     7501 gcaacttggc ctgcggctac catgtgtcct cgcacggaga gagtggaaat gagcaagggg
     7561 cgcctcgagg catttagcga cggagtgatc gcggtgatca tcacgatcat ggtgctggag
     7621 ttgcaagcgc cgcacggcgc caccctgtcc gaccttaagc cgaaaattcc cgcgtttctg
     7681 agctacgtgc tgagttttat tttcatcggc atttattgga acaatcatca tcatttgctg
     7741 cacgcgataa ccagagtgaa tggccgcgtc ctctgggcga atcttcattt gctattttgg
     7801 ctgtcgctga ttccgtttgc cacggattgg atgggcgtga atcagtgggc cactgtgccg
     7861 gtggcggttt acggctgcct gttgttcatg gccgccgcgg ctttcaccat tcttgcttac
     7921 gaactgatcg ctctcgaagg aaaggattcc acactggcgc gtgcgctggg gcgcgattac
     7981 aaaggcaaaa cttctttatt actttacgca ttggccatac cgctcgccta tttaaatccc
     8041 cgaatttcca ctgcgattta tgtcctcgtc gcattcattt ggctcattcc cgaccgccga
     8101 atcgaaaaag tggttgcgga acgatgattc gcgtcgctac agattagaaa tttcaattta
     8161 aatgccgtct caatcagatc caaatccaaa tgtaatgaag ttcgggttga tgtacgatgg
     8221 tcagcacggg tgaacgtaaa atgcaaatct ctgctcaggt ctattgcgat gtgtgtttaa
     8281 tcgaggattt aaatcgtgat tctgatttcg attcttcatg cacctcaagc aaggccagtc
     8341 aaaaatgaaa ttgaaatgga tttcctgcat cgtttgtttt cttggaataa tattttgtca
     8401 ttttattcct ttgctggttg cggcgcagaa tcagtccacg caaaccacta ccgcgcaaga
     8461 tccggctccg caggaatccg ctccccgcga gctgtggtct tttctcaaag aagggaaaat
     8521 atcatttagc acccggtggc gatttgaatc cttcgagcgg gacggggcgc cgttcaccgg
     8581 aatcgcggag gcccccacgc tgcggattgc gttgggttac gaatctcctg agtatcatgg
     8641 cttcaccgct tttgcgcagg gagaatccgt gatcgtcacg ggtgccgcca actacagcat
     8701 tcctttgcgg ccctcgcaaa atcaccccaa tcttccttcg atcctcgatc cgaaaagcct
     8761 ggaaatgaat caggcgtacg taaaatggga gcgccatatt tcggataaaa atttttcgct
     8821 gacggcaggc cgccaggagc ttacgctgaa cgacgggcgc tttattagtt attccaactg
     8881 gcgccaggtc cacggatcgt ttgacgcggt ccgtttggaa gccgaacttc cacggaattt
     8941 ttcagttacc tacgcattta tcaatcgctt ctaccgtgaa gatggttacg atgctcttga
     9001 cggcgaagag ccgatgcaca cggacatggt gaatttgaag tggaataaag ccgacaaatt
     9061 gaatgcctcc gtgtatgccc tgctgcttaa ttttcgaggt cctgctcaat ttgcaacgtc
     9121 cacacaaacg tatggagcgc gcgtgaccgg tccataccag ttcaccccgg actggagcct
     9181 tttgtacgtc gcggaatttg ccaagcaaaa aaactatggc acgaatccaa acaaggagga
     9241 cgaaaattat tatctgggcg agatcggtcc tggctggcgt gatttcagcg tgaaggcagg
     9301 ctatgccttc ttgggaggac gcagcgctac cgatttgtta accactccgc ttgcacctcc
     9361 tcgcaacggt tggacggatt tgtttttcac taaccccagt attactggcg gccacggatt
     9421 ggaagccgcc tacgtgaatg ccagcggacc aatacgattt cttgacggcg ccattgctac
     9481 ttttatttat tacgactatc actccgacta tccccacatc cactacggca gtgaactgga
     9541 ttcttcgctg gcatacaaaa tcaaaagaat cagcaatcga tgggaaatag gctggagatt
     9601 tgggcgttac tggtcagatc acttgtattc aaattcgctg cgcacttcgg tctataccat
     9661 tttcactttg taaacgcggg cacatcaagt agcacaggct tcagcctgtg gaatttagtc
     9721 cttgcaaggc caaaactcca caggctgaag cctatgctac taaatacaat tcttcctacc
     9781 cgccgcgagt atgcatttcc aggtgcggat ctttccgaag ctgttcgatc ccaatcagca
     9841 cgccgcgctg gcccgttgcg gcgcgcagtt cggcgccgcg atcggtgcgc gcctgccaaa
     9901 tggaagaaat tcgagcggca agttcttcct tggtggctcc ttcgcgcaga attttgcgga
     9961 gatcgattcc agcttgcgcg tagaggcaca aataccagag accgtcggcg gtcaagcggc
    10021 tgcggtcgca tgtcttacag aatggcgtcg tggtggaggc gatgatgccg aacacggtgc
    10081 cgtccggcag ctggaaccgc tccgctgggg cggatgaatt ttcgttgagc gcagtaatcg
    10141 tcccgtagcg tttgctgagc acgtcgagca tttcagcgcg cgtaaaaact ttttccatcg
    10201 accattctgt cgcgccgccc acgtccatat actcaatgaa tcgaacttcg gcctcaacgc
    10261 cgcgtccgta ctcgatcaaa ttagctagct catcttcatt cacgccgcgc agaacaaccg
    10321 tatccaattt cagtttggga aagccaagtc gccgtgcagc cgcaatccca gcaagcacct
    10381 cggcgtgcat gtcgcg
//
LOCUS       DASZMB010000138         4723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1783275, whole genome shotgun sequence.
ACCESSION   DASZMB010000138 DASZMB010000000
VERSION     DASZMB010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1783275"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="VG272_06425"
     CDS             complement(<1..922)
                     /locus_tag="VG272_06425"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HWF13352.1"
                     /translation="MKLVNPANRRKHTVIIVGSGLAGASAAASLGQQGYNVHCFCFQD
                     SPRRAHSIAAQGGINAAKNYRNDGDSIHRLFYDTVKGGDFRAREANVFRLAQISVNII
                     DQCVAQGVPFAREYGGLLDTRSFGGVQVSRTFYARGQTGQQLLLGAYQGLEEQIATGA
                     VKMHTRSEMLDVVVVNGRARGIVTRNLLTGEIETFAADCVVLATGGYGNVFYLSTNAK
                     GCNTTAIWRAHRRGAFFGNPCFTQIHPTCIPVSNENQSKLTLMSESLRNDGRVWVPKK
                     SGDTRPPSQIPEDERDYYLERRYPSFGNLVP"
     gene            complement(1044..1763)
                     /locus_tag="VG272_06430"
     CDS             complement(1044..1763)
                     /locus_tag="VG272_06430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HWF13353.1"
                     /translation="MTDTTADTKAAPNRLILLWNSVIGKKVVMAVTGAVLVLFVISHM
                     VGNLKIFSGPDEINAYSRFLRTVGYPELGYGQLLWLVRGVLLLSVFLHIVAATQLTIM
                     NRQARPVGYGTKKNVETSWGALTMRWGGVLLAIFIVFHLFHLTGGVVGFQPGQFEHLA
                     VYQNVVAAFQVVPISLFYIVAMFALCLHLDHGIWSGLQTLGWVNLTNTKMLRAISRVI
                     ALIIFVGFISVPISVLAGWVR"
     gene            2119..2652
                     /locus_tag="VG272_06435"
     CDS             2119..2652
                     /locus_tag="VG272_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13354.1"
                     /translation="MVDQQVADLDALVEELEKSATAPDSEALLKLANILAKPFDVKTD
                     EVAVFKLVPKYKSLKFVIPEKLTPVGTIPLTSTTALAARTARDRKGELANNFGTSRHA
                     NVFEAVPLGRAPSEMIQKIISAPILDGKAIHGVVQICRKAGTLAGAGPDFTQKDLRAL
                     AALSPMLDRFLRLCKID"
     gene            3033..3590
                     /locus_tag="VG272_06440"
     CDS             3033..3590
                     /locus_tag="VG272_06440"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF013979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HWF13355.1"
                     /translation="MAFVAKKIYLTKGVGKHRERLSSFELALRSAGIAACNIVRVSSI
                     FPPNCKLISRAEGLKHLKPGQVAFTVISENSTREAHRLIAAGIGLALPADKSMYGYLS
                     EHHSFGETEEVAGEYTEELAAEMLATTLNVEFDPDLSWDEKKEVYRISNKIVRTMNIT
                     QSAVGDKRGLWTTVLAAAIMIGEDD"
     gene            3726..4139
                     /locus_tag="VG272_06445"
     CDS             3726..4139
                     /locus_tag="VG272_06445"
                     /inference="COORDINATES: protein motif:HMM:NF025471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4279 domain-containing protein"
                     /protein_id="HWF13356.1"
                     /translation="MHTYTVALRISGADLDLAEVTSALKLKPTQTRVIGQKRDSSDFQ
                     WPESMWEYEARPSESKFEWNTLEAGLQAVVLTFASREKQLRIYKQRFKVILWCGHFSS
                     SFGGGPTLSPQILISLADLGVELKLEAYISDEPTP"
     gene            4314..>4723
                     /locus_tag="VG272_06450"
     CDS             4314..>4723
                     /locus_tag="VG272_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HWF13357.1"
                     /translation="MTQKFKVGLIQMTCSLDPAENLAKVEARVREAAERGAQIVCTQE
                     LFRSQYFCREENAELFDLAEPIPGPSSEALAKLARDLKIVIVGSLFERRAAGIYHNTA
                     VILDADGSLLGKYRKMHIPDDPLYYEKFYFTPGDL"
BASE COUNT         1108 a         1365 c         1203 g         1047 t
ORIGIN      
        1 gtggcacgag gtttccaaaa ctcggatagc gccgctcgag ataatagtca cgctcatcct
       61 ccggaatctg cgacggcgga cgtgtatcgc cgcttttctt cgggacccaa acgcgtccgt
      121 cattgcgcag cgattcggac ataagcgtga gtttggattg attttcattg ctcacgggaa
      181 tgcacgtcgg atgaatctgt gtgaagcagg gattgccgaa aaatgcgccg cgccggtgcg
      241 cgcgccagat ggccgtggtg ttgcagccct tcgcgttcgt agaaagataa aagacgttgc
      301 cgtagccgcc cgtcgcaagc acgacgcaat ccgccgcgaa cgtttcgatc tcgccagtca
      361 gaagattgcg cgtcacgatg ccgcgagcgc ggccgttaac gaccacgacg tcgagcatct
      421 cgctgcgcgt gtgcattttt actgcgccgg tggcgatctg ctcttccagg ccctggtacg
      481 ctccgaggag aagctgctgc cccgtctgtc cacgcgcgta aaacgtacgc gatacctgca
      541 cgccgccgaa cgaacgtgtg tccagcaacc cgccgtactc tcgcgcgaac ggaacgcctt
      601 gtgcgacaca ctggtcgata atgtttacgc tgatttgcgc gaggcgaaac acgttggctt
      661 cgcgcgcgcg aaaatcgccg ccctttaccg tgtcgtaaaa caggcggtga atgctgtcgc
      721 catcgttgcg atagtttttc gccgcgttga tgccgccctg cgcggcgatc gagtgcgcgc
      781 ggcgcgggct gtcctgaaag cagaagcagt gtacgttgta gccctgctgg ccaagcgacg
      841 cggccgccga cgcgccggca agtcccgagc ccacgataat caccgtgtgc ttgcggcggt
      901 tcgccggatt tacaagcttc atctcaaagc gggatttttc ccacttctgt tcaattggtc
      961 cggacggtat atgtgaatcg agcatttctc tccagtgtgg cggtgtcgca cgtgcagggg
     1021 cacgacgtgt cgtgcctcta cttttagcgc acccagcccg caagcaccga tatcggcacc
     1081 gagataaatc caacaaaaat aatcagagca atcactcggg aaatggcacg cagcatcttg
     1141 gtattcgtca gattcaccca gccaagagtc tgcaaaccgc tccatatccc atgatcgaga
     1201 tgcaggcaca gcgcgaacat cgccacgatg taaaacagcg aaatgggcac aacttgaaaa
     1261 gcagcgacga cgttttggta aacggccaaa tgttcaaact gtcccggctg aaaaccaacc
     1321 acgccacctg tcaaatgaaa caggtggaac acgatgaaaa tcgccagcag cacgccgccc
     1381 cagcgcatgg tcaacgcgcc ccacgaagtt tccacatttt tcttcgtgcc gtagcccacc
     1441 gggcgcgcct ggcggttcat gatcgtgagt tgcgtggccg caacaatatg caagaacacg
     1501 ctcagcagca gcacgccgcg cacaagccac aagagttgcc cgtaacccaa ctccggatat
     1561 cccaccgtgc gtaggaagcg cgaatacgcg ttaatctcat ccggtcccga gaatattttt
     1621 agattgccaa ccatgtggga gattacaaaa aggaccagca ccgccccggt cacggccatc
     1681 acgacttttt tgccgatgac gctgttccag agaaggataa ggcgattcgg ggccgctttt
     1741 gtatccgccg tcgtatctgt cattgctgaa ctcctgaatt tatttccgtg gctttctttg
     1801 cgcctcacgc gttgcggcgc accctgctca tcgcgacaca ccctgcgcat ccctgcccgt
     1861 cagccgtcca taatttctat ggcagtttct gtaacttctt gaagggattg gcccgtggga
     1921 aaattaggcg atgtcgaaaa tccacttcga tcgctgacat tccggctccg atgaacttga
     1981 aaactcagta taggtaattc ctgctccatt ttcaatggcg atgatattgg attggccaca
     2041 cggctcacaa cgccccgaat tgttttagtc acgttttcgg tataatcgcg ttggccccct
     2101 ctactggaga aaccgttcat ggtggatcaa caagttgcgg atttggacgc gctcgtcgaa
     2161 gaacttgaaa agagcgccac agcgcccgat tccgaagcct tgctcaaact ggcgaatatt
     2221 ctggccaaac cttttgacgt gaaaacggac gaggttgccg tcttcaaact cgttcccaag
     2281 tataagagtt taaaatttgt gattcccgaa aagctgactc ccgtgggaac gattcccctc
     2341 accagcacaa cggccctagc tgcgcgcact gcgcgggacc ggaagggcga acttgccaac
     2401 aattttggca cgtcccgcca tgcgaatgtg tttgaagctg ttcccctggg acgagccccg
     2461 agcgaaatga ttcagaaaat tataagcgcg cccatcctgg acggcaaagc cattcacgga
     2521 gttgtgcaga tttgccgaaa ggccggcacg ctggcgggcg caggccctga ttttacgcag
     2581 aaagatcttc gcgcactcgc cgccctttcg cccatgctgg atcgcttcct cagactctgc
     2641 aaaattgatt aatgggctca cgtggcgcgg ggtttcacag gttgcggcaa aagaaaaaac
     2701 cgtcattccg ggacatgcaa tttgccgagg aatctctctt tctctgggac tgccatcgga
     2761 atgacgatca atcctcaagt agcacaggca ctcttgcctg tgtgcatttt gcaaaaccca
     2821 cacaggcaag agtgcctgtg ctactcaaac atcgcccgaa ccatttttcc gaatgtctct
     2881 attcttcaca agttgcctgc aatcttgcgc ttcgccaggg aaagtaattg acacacgtgc
     2941 ctgttcttgt tacttttccg cacgcactaa aaatgagcaa aaatttctca gaagtcactt
     3001 tgtcgtagga ggcagcgcac tcccgcaccc tcatggcgtt cgtcgcgaaa aagatttacc
     3061 tgacaaaagg tgttggcaaa catcgagaac ggctttccag ctttgaattg gcactgcgca
     3121 gcgcgggtat cgccgcgtgc aacatcgtgc gcgtctcgtc catcttcccc ccgaattgca
     3181 agctgatctc gcgcgccgag ggcttgaaac acttgaagcc cgggcaagtc gcgttcacgg
     3241 taatcagcga aaattccacg cgcgaggcgc accgcctgat cgccgcgggt atcggcctgg
     3301 cactccccgc cgataaatcg atgtacggct atctgtccga gcaccactcc ttcggcgaaa
     3361 ccgaagaagt cgccggcgaa tacaccgaag agctcgccgc cgaaatgctc gcgaccactt
     3421 tgaacgtgga attcgacccc gatctatcct gggacgagaa aaaagaagtt taccgaatct
     3481 ccaacaaaat cgtccgcacc atgaacatca cccagtccgc cgtaggcgac aagcgcggcc
     3541 tatggaccac agtcctagcc gccgccatca tgatcggcga agacgactaa tactctggct
     3601 ctctgacgtt ccattccact atcacgtatt gacagatttt ttcgcctgtt gcgcctcggt
     3661 tccaaatgtc ttgtggtgcg ttcgaccaca atctagcgcc tgatgctaaa atctcattct
     3721 tgcccatgca cacatacacg gtggcattaa gaataagcgg tgccgatctc gatttggctg
     3781 aagttactag cgcgttaaaa ctcaagccaa cccaaacaag agttataggg caaaaacgag
     3841 attcgtccga cttccaatgg cctgaatcca tgtgggagta tgaagcgaga ccgtcggaaa
     3901 gtaaattcga gtggaatacc cttgaggctg ggctacaggc agtagttctg acgtttgcgt
     3961 cacgtgaaaa acaattgcga atttataaac aacgattcaa ggtgatttta tggtgcggcc
     4021 acttttcgag cagcttcggc ggcggaccga ccctgtcacc gcaaatatta atatccctcg
     4081 ctgatctcgg tgttgagctt aagcttgaag cttacatttc agatgaacca acaccgtagt
     4141 aatccgtaca tgccgggtgc cgcacccttt gtgattttca aagggtgcgg ggttagatga
     4201 cagtgccatc gtcgcgcaca aacttggcta gactttcgtg aattttagcg gctcctacca
     4261 aatctcaata cctcgcttct tccaatcgaa acagttaatg ctatgattcc tgcgtgacgc
     4321 aaaaatttaa agttggactc attcagatga cctgctccct cgatccggcg gagaatctcg
     4381 ccaaggtcga agcgcgcgtt cgcgaagcgg ccgagcgcgg cgcgcaaatt gtctgcactc
     4441 aggaactgtt ccgctcgcaa tacttctgcc gtgaagaaaa cgctgagcta ttcgacctgg
     4501 ccgaaccgat tcccggccca tcctccgaag cgctggccaa actcgcgcgc gatttgaaaa
     4561 ttgtaatcgt cggttccctc ttcgagcgcc gcgcggcggg catttatcac aacacggccg
     4621 tgattctgga tgcggacggg tcgctcctcg gaaaatatcg caagatgcac atcccggacg
     4681 atccgctcta ctacgaaaaa ttttatttta ctccgggcga cct
//
LOCUS       DASZMB010000139         4491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1519014, whole genome shotgun sequence.
ACCESSION   DASZMB010000139 DASZMB010000000
VERSION     DASZMB010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519014"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VG272_06455"
     CDS             <1..273
                     /locus_tag="VG272_06455"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWF13358.1"
                     /translation="NILIFPEGDLTPDGKLQTFRAGIGLLAGDLKIPVVPMRIDGAYE
                     IREVRSLFNRPGSVRVYIGKPVEFPVGSDAQQITRTLQKIVAELGQ"
     gene            complement(391..2223)
                     /gene="typA"
                     /locus_tag="VG272_06460"
     CDS             complement(391..2223)
                     /gene="typA"
                     /locus_tag="VG272_06460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HWF13359.1"
                     /translation="MPQIRNIAIIAHVDHGKTTLVDAMLHQSGVFRDNQVVMKRVMDS
                     NDLERERGITILAKNTALFYKDTKINIVDTPGHSDFGGEVERALKMVDGVLLLVDASE
                     GPLPQTRYVLQKALHAKLLPIIVLNKIDRSDARIAAVLDEIYDLFIDLEATEDQLDFP
                     VLYTNAKLGVAHRKLDDGSENLQPLFETIVSAIPEPKGDPNGVLQAQVMNLDYSDFLG
                     RIAIGRVFNGTIRRGSDVGIAKRNGEIVPTRITKLYTFRGLERDEAEEVPCGDLAAIA
                     GVEGIQIGESITSVENPAPLEPLVIDEPTLAMNFTINTGPLSGREGQWVTSRDLRARL
                     KKELLTNVSLRITDSEATDIFRVFARGELQLAILIETMRREGYELMVGKPEIVIREEN
                     GKKLEPLERLVVDCPEAFVGVVMESLGRRRGELGKMVNHGSGWVRMEFTVPSRGLIGL
                     RGQLLTDTRGTVQLHTLFEGWTEYGGEMASRPTGALVADRAGVTTAFALWNLQERGEL
                     FVGPGEEVYEGMIVGENARDADMDVNVTKEKKQTNMRASSADEAIRLIPHRQLSLEQA
                     IEFISDDELVEVTPKSIRLRKKILDSKKRPKRWQQIRADAEAKL"
     gene            2686..3162
                     /locus_tag="VG272_06465"
     CDS             2686..3162
                     /locus_tag="VG272_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13360.1"
                     /translation="MRKLIYLCIAVALSVPLISFAAGGDLAVVVNKANATSNLTKSEL
                     RKLVLGEQESWPGGMKVTVTLRAQGNPERIAVLKVVCRMSEDEYIQHSMHSDFVGTST
                     NAPRIVASAAAVRQMVSSVPGAIGFLPLSEVNDTVKVVSVEGISAGEPDYKIKVGQ"
     gene            3165..4004
                     /locus_tag="VG272_06470"
     CDS             3165..4004
                     /locus_tag="VG272_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HWF13361.1"
                     /translation="MKGTIRNKLTAGFSGVLILMAIVAMIGGYAVFSLRHDANDATRV
                     GARLNSLALEIQIHDLEASRKVNNYLAQTGTADGDKKRKMYLEEAKFEINEIQTLASK
                     AVEIAPTSASRVKFTKIREASAQFGDAIEKVVTATDTESIAAKDAKSAYEDAADELHE
                     SAEDGEVVGHDASQASLEAIDRTSSRSVALVVGISVLGLILGITVSYKLARAILIPVD
                     HLKEVAENVSLGNLDMSVHRYSDDEIGDLADSFSRMVTAVKFFRMEAQLGQSEEAAHE
                     VKH"
     gene            4057..>4491
                     /locus_tag="VG272_06475"
     CDS             4057..>4491
                     /locus_tag="VG272_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13362.1"
                     /translation="MNVLRTVFFKKTGNGLILGAVTMMFIVLLHGTPAKAQNKDGQPQ
                     IAETIAAKLTEAEAVFQRTEAQIASRNMGEPAPENTRQSEATDSASGTASGIAPMAAM
                     PLQNGQTGASAPAPATQQEPVPAGPPPSEEESHEHMMEIPHGP"
BASE COUNT         1071 a         1145 c         1241 g         1034 t
ORIGIN      
        1 aatattctta ttttccccga aggcgatttg acgccggacg gcaaattgca aacattccgc
       61 gccggaattg ggttgcttgc gggcgatctg aaaattcctg ttgtgccgat gcggattgat
      121 ggcgcatatg aaatccgcga ggtgaggagc cttttcaatc ggccgggaag tgtgcgcgtt
      181 tacattggca agcctgtgga gttcccggtg gggtctgacg cgcaacagat tacgcggacg
      241 ctgcagaaaa tcgtagcgga attggggcaa tgaacattgg ggaattaagt tgttagagag
      301 acgatgtcag aagaccgtgg cataggcttc cgcctgtgcg agtttagttc ttgcaaggac
      361 taaccccgca caggctgaag cctatgccac ttaaagcttt gcttcggcgt cagcgcggat
      421 ctgctgccag cgcttgggcc gttttttgga gtcgagaatt ttcttgcgta ggcggatgga
      481 tttcggcgtg acttcgacca gttcatcgtc ggagataaat tcgatggctt gttcaaggct
      541 cagttggcga tgagggatca ggcggatggc ttcgtccgcg ctggatgcgc gcatgttcgt
      601 ttgcttcttt tctttcgtga cgttgacgtc catgtcagcg tcgcgcgcat tttcgccaac
      661 gatcatgcct tcgtaaactt cttcgcccgg gccgacaaac aattctccgc gctcctgcaa
      721 attccacaaa gcgaaagcag tggtaacgcc agcacggtcc gcgacgagcg cacctgtcgg
      781 gcgtgaggcc atttcgccgc cgtattccgt ccagccctca aacaatgtgt gcagttggac
      841 tgttccgcgc gtatccgtca gcaattgtcc gcgcaaaccg atcaagccgc gcgaagggac
      901 ggtaaattcc atgcgtaccc atccggaacc gtgattaacc attttgccaa gttcaccacg
      961 gcgacgtccg agagattcca tcacgacgcc gacaaacgct tccgggcaat cgacgacgag
     1021 tcgttcgaga ggctcgagtt ttttcccgtt ttcttcgcgg atcacaattt ccggtttgcc
     1081 aaccatcaat tcgtagcctt cgcgacgcat ggtttcgatc aatatggcca gctgcaattc
     1141 gccgcgcgca aacactcgaa aaatgtcggt cgcttcggaa tccgtaatgc gcaaagaaac
     1201 gttggtgagt aattcttttt tcaatcgagc gcgcaaatca cgcgatgtga cccactggcc
     1261 ctcgcggccg gagagcgggc cggtgttaat cgtgaaattc atggccaaag taggttcgtc
     1321 aatgaccagc ggctcgagcg gggcgggatt ctccacactg gtgatgcttt cgccaatttg
     1381 aattccttca acgccggcga tggcggcgag atcgccacag ggaacttcct cggcttcgtc
     1441 gcgttccagg ccgcgaaatg tatacaactt ggtgatacgc gtggggacaa tttcgccgtt
     1501 gcgcttagcg atgcctacat cggagccgcg gcgaatggtg ccgttaaaca cacgtccaat
     1561 cgcgatgcgt cccaggaaat cgctgtaatc caggttcatg acctgcgctt gcagcacgcc
     1621 gttcggatcg cccttgggct ccggaattgc agacacgatc gtctcaaata gcggctgcaa
     1681 attttcggag ccgtcatcca attttctgtg ggcgactccc agctttgcat ttgtgtagag
     1741 aaccgggaag tccagttggt cttcagtggc ttcgaggtcg atgaataggt cgtaaatttc
     1801 gtcgagcacg gcagctatac gcgcgtcgct gcggtcgatt ttattcaaca cgataatagg
     1861 taaaagtttc gcatgcagcg ctttctgcaa aacgtagcgc gtctggggca gcggaccttc
     1921 gctcgcgtcc acgaggagca atacgccatc caccattttc agggcgcgct cgacttctcc
     1981 gccgaaatcg ctgtggcccg gcgtgtccac gatattaatt tttgtgtctt tgtaaaacag
     2041 cgcagtattt ttggccagaa tggtgattcc gcgctcgcgt tcgagatcgt ttgagtccat
     2101 cacgcgcttc ataacgacct ggttgtcgcg gaagacgccg ctttggtgaa gcatggcatc
     2161 caccagcgta gttttgccgt ggtcgacgtg cgcgatgatg gcgatattgc gaatctgtgg
     2221 catggaatcc taactttctc ttcgattgtc gtttcggcgc ccggtgcggt cctgtagcaa
     2281 acagttccgc cggaataagc aatgaaaaca aaaagagctg caagtgcgga cttgcagcct
     2341 ccacgttcgt ttccaagagg aattacaagt gccttcgacc aaggtagagt accacattaa
     2401 ctctctctga gtcaccgctg aagacgcggc cttggggctt cttttcccct tgtggtagcc
     2461 cccaagtgcc tttttgggtc atgtatcaag ttcgtgagaa tcgttgccga tagcttattg
     2521 aaggacaaat aagaccccct ttcttaacca tcagtaatac taatttttaa aaaatttatc
     2581 ggttgagatt ttgcggattg gaatcgaagt gctctaaaga cctgacagat tatgtcgaga
     2641 ttaaaggaaa gcaaagtatt taactggaag caaaagggcg gtgagatgcg aaagctaatt
     2701 tatctgtgta tcgctgtagc actatccgtg ccactcattt cattcgctgc gggcggcgat
     2761 ttggccgtcg tggttaacaa agcaaatgcg acaagtaatt tgacgaaatc ggaattgcga
     2821 aaactcgttc tgggagaaca ggaatcatgg ccaggaggga tgaaggtaac tgtcacgctg
     2881 cgtgcgcagg gtaaccctga gcgcattgcc gttctaaaag tggtttgccg gatgagcgaa
     2941 gacgaataca tccagcacag catgcattct gacttcgtgg gcacgtcgac gaacgcaccc
     3001 cgaatcgttg cttccgcagc tgccgtgcgg caaatggtgt ccagcgttcc tggggcaatt
     3061 gggttcctgc ctctcagcga agtgaatgac acggtgaagg tggtgagcgt ggaaggcatt
     3121 tctgccgggg aaccggacta caaaataaag gtgggtcaat aaccatgaag ggaaccattc
     3181 gcaataaatt gaccgccgga ttttccggtg tgctgatcct gatggcgatc gttgccatga
     3241 tcggcggata cgctgtattt agcctgcgcc acgatgcgaa cgatgccacg cgtgtgggag
     3301 cgcggctaaa ttcactggcg ctcgaaattc agatccacga cctggaagcg tcacgcaagg
     3361 tgaacaacta cctcgctcaa accggaaccg cagacgggga taagaaacga aagatgtatt
     3421 tggaagaggc caaatttgaa attaacgaaa tacaaacatt ggcctcgaaa gccgtagaaa
     3481 ttgcgcccac cagcgcaagc cgcgtcaagt tcacgaaaat cagggaagcg tccgcgcaat
     3541 tcggcgatgc gatcgagaaa gttgtgactg ccacagatac tgaaagcata gccgccaagg
     3601 atgccaagtc cgcatacgaa gatgccgccg atgagttgca cgaaagcgcg gaagacggcg
     3661 aagtggtggg gcacgatgcc agccaggcct ctctcgaagc gatcgaccgg acgagttctc
     3721 gatccgtcgc cttagtggtt ggaatttctg tcctcggatt aattcttggc atcacggtca
     3781 gctacaagct agctcgcgcc attcttattc ctgtcgacca tttaaaagaa gtagccgaaa
     3841 acgtaagcct cggaaatttg gacatgtccg tccaccgata ttcggatgac gaaattggag
     3901 accttgccga ttccttttcg cgcatggtta ccgccgtgaa attttttcga atggaagctc
     3961 agctcggtca atccgaggag gccgcccacg aagttaaaca ctgagcggga tcactcagca
     4021 gcaaatccag tggagatgtt cgagggggag acgcgtatga atgttttgcg cactgttttt
     4081 tttaagaaaa ccgggaacgg attaatcctt ggggcggtga ccatgatgtt catcgtgtta
     4141 ctgcatggaa ctccggcaaa agcacagaac aaagatggac agcctcaaat cgcggaaacg
     4201 attgccgcga aattgacgga ggcggaagct gtgtttcagc gcacagaagc gcaaatagcc
     4261 tctcgaaata tgggtgaacc ggctcctgaa aataccagac agagtgaggc aacagactcc
     4321 gcatcgggca ccgcatcggg catcgcgcca atggctgcca tgccgcttca aaatggacaa
     4381 actggagcga gtgctcctgc gcctgcaacg caacaggagc cagtccccgc tggacctcca
     4441 ccatccgagg aggaatcgca cgagcatatg atggaaattc ctcacggacc c
//
LOCUS       DASZMB010000140         2839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4273732, whole genome shotgun sequence.
ACCESSION   DASZMB010000140 DASZMB010000000
VERSION     DASZMB010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4273732"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VG272_06480"
     CDS             <1..607
                     /locus_tag="VG272_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13363.1"
                     /translation="ALPIWSQAASSGGPMRVESTFFVEESELRKPITIVAYGDMRFTD
                     PATLPPENVTARRALVGRIAQEKPDAVLLNGDLTMHGGDLGDYATYHTETKRASRASH
                     ARFVVIAGHIHNYERFERDGVVYLVSGGGGAPQQTIDREPDDLFRANSYPNFHYLKFT
                     LSGATLDATMYRLANTNSAVWEAKDTFRVRARTGQSHKSLN"
     gene            941..1270
                     /gene="cyaY"
                     /locus_tag="VG272_06485"
     CDS             941..1270
                     /gene="cyaY"
                     /locus_tag="VG272_06485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HWF13364.1"
                     /translation="MDEKEFQKRCDIAFESLRRRLQEAGDAHGFEVEGGSGKLEILFE
                     DDDETRFVISPNTPVRQIWISALTTSFKLSWSDAAGTFVLEKTGETLMQLMGRILTEQ
                     LGSPVSV"
     gene            1276..1920
                     /locus_tag="VG272_06490"
     CDS             1276..1920
                     /locus_tag="VG272_06490"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HWF13365.1"
                     /translation="MDLRPLRSILQERPRRSLRNLSSAALLAVFALTALFFAEPLQAA
                     PNPQAGSVLQPQAYVSLQPVPRDHAFEIAVIAKITPGFHVNAHQPSEDYLIPTKITAD
                     LPPGILVVETSYPRGVMRGFSFTKEQLRVYEGSFTVRMKLRAQNSARIGKQKIGLTIG
                     YQACNQDSCLPPTKVSTTADLEVADSDTPAHPANADIFSSKSEPAPAVKFPPHR"
     gene            complement(1975..>2839)
                     /gene="msrP"
                     /locus_tag="VG272_06495"
     CDS             complement(1975..>2839)
                     /gene="msrP"
                     /locus_tag="VG272_06495"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003767.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HWF13366.1"
                     /translation="TGVGLRFVKPTMPLLAPDMGYGPSRLEGVIPGRYSTKEQQTPYF
                     FVTHYNNFYEFGSEKDQPVENARNLRTSPWTVTVNGEVSRPRTFSMDEIVKLVPLEER
                     IYRHRCVEAWSIVVPWIGYSFSALVKLVQPTSRAKYVAFESYFDLERMPEAPYAGIDF
                     PYVEGLRLDEALHPLTLLCVGMYGEELPKQDGAPVRLVIPWKYGIKSIKSLVKISFVQ
                     EQPRATWNAHNPHEYGFYANVNPQVDHPRWSQEKERRLGELGKRPTLMFNGYADQVAK
                     LYSGMDLRKYF"
BASE COUNT          633 a          795 c          749 g          662 t
ORIGIN      
        1 ggccttacct atttggtccc aagcggcttc ctcgggcggt ccgatgcgcg tcgaatcgac
       61 tttcttcgtg gaggaaagcg aattgcggaa acccattacg atcgtagcct atggcgacat
      121 gcgctttacc gaccctgcca ctctgcctcc cgaaaacgtg actgccaggc gcgcattagt
      181 gggccgtatt gctcaagaaa aaccggatgc cgttctactc aacggtgatc tgaccatgca
      241 cggaggggat ctcggcgact acgccactta ccatacagag acaaaaaggg cgagccgtgc
      301 gagccacgca aggtttgtcg tcatcgccgg tcacatccac aattatgagc gttttgaacg
      361 cgatggcgta gtttatctgg tttctggcgg cggaggggct ccgcagcaaa caatcgatcg
      421 tgaaccggac gatctgtttc gggccaattc ctacccaaat tttcactatc tgaaattcac
      481 attaagcggc gccacgttgg atgcgacgat gtatcgtctg gccaatacga attctgcggt
      541 gtgggaagcg aaggacacct tcagggtaag ggcaagaacg ggccaatcgc acaaaagtct
      601 aaattaatag cagaaattcg gccgattata agcggacgac tagattagac tcagcgtcag
      661 gaaaaatttg cggcaggctg gacagaactc gcttttcttc atcgatacgc cgcaccgccc
      721 gcagcgtttt tgcgaagtgc gaagcgattt tcgcgcacta gcggcaacaa catttttcat
      781 agcgtctcac gaccgacatt attatgggcg atggaagctg gccatagggc aaatgctgta
      841 attgccaggg gacaatgatt tatctcgttc aatcttccga tttccacacg ctcttcgatg
      901 ccttgcccgg ctcaacgctt cgcggtactc tgttgttacc atggatgaaa aagaatttca
      961 gaaacgttgc gatattgcgt ttgaatcttt gcgccgccgc ctgcaggaag cgggcgatgc
     1021 gcacggattt gaagtggagg gcgggagcgg caagctggaa attctgttcg aggacgacga
     1081 cgaaacgcgc ttcgtcatca gtccgaacac ccccgttcgt cagatatgga tttccgcgtt
     1141 gactaccagt ttcaagcttt cgtggtcgga cgcagcggga acctttgttt tggaaaaaac
     1201 cggcgagacc ctgatgcagc taatgggccg cattttaacg gaacaactcg gcagtccggt
     1261 gagcgtgtga tgcatatgga ccttcgaccc cttcgctcga tcttgcaaga acgcccgcgg
     1321 cgctcgctgc ggaatctcag ttcagcggcg ctgcttgcgg ttttcgcgtt gacggctctt
     1381 ttcttcgctg agccgctcca agccgcaccc aatcctcagg ccggcagcgt gctgcagccg
     1441 caggcttatg tttcgctgca accggttccg cgggatcatg catttgagat tgctgtgatc
     1501 gcaaaaatca ctcccggatt ccacgtcaat gcgcatcaac cttccgagga ttatctgatt
     1561 cccactaaga taaccgccga cctgcctcct ggaatcctgg tcgtggaaac atcatatccg
     1621 cgcggagtca tgcgcggttt cagctttacc aaggagcaac tccgcgttta cgaagggtct
     1681 ttcaccgtcc gcatgaagtt gcgcgcgcaa aacagcgcgc ggatcggcaa gcagaagatc
     1741 ggcctcacga tcggatatca ggcttgcaat caggactcgt gcctgccgcc cacaaaggtt
     1801 tccacgacgg ccgatttgga agttgcagat tcggacacgc ccgcgcatcc ggcaaatgcc
     1861 gacatatttt cttctaagtc tgagcctgcg ccagccgtga aattcccccc gcatcgttaa
     1921 ctttggcatt aattcctgtg aaacccagcg ggccggcacg cggcggtttt ctgatcaaaa
     1981 atattttcgt aggtccatgc cggaatacaa cttcgccacc tgatcggcat atccgttgaa
     2041 catcagcgtg ggccgctttc ccagctcgcc caggcgtcgc tctttttcct gactccaacg
     2101 tggatgatcc acttgaggat tcacgttcgc ataaaatccg tattcgtgag gattgtgtgc
     2161 gttccaggtt gcgcgcggtt gctcttgcac gaagctgatc tttaccaaag atttgatact
     2221 tttgattcca tatttccagg gaatcaccag gcgaaccggc gctccgtcct gtttgggaag
     2281 ttcttcgccg tacattccca cgcagagcag cgtcagagga tgcaaggcct cgtccaggcg
     2341 caaaccctcc acgtaaggga aatcgattcc agcatagggc gcttcgggca ttctttccaa
     2401 gtcaaagtag ctctcaaaag cgacatactt agccctggat gttggttgaa cgagtttcac
     2461 tagcgcgcta aacgagtatc cgatccacgg cacgacgatc gaccacgcct ccacgcagcg
     2521 gtggcggtaa atacgctctt ccagcggcac cagcttcacg atttcgtcca tgctgaaagt
     2581 gcgcggcctg ctcacttctc cgttgacggt gaccgtccat ggcgaagtcc gaaggttccg
     2641 cgcattttcc accggctggt ctttctcaga accgaattcg taaaaattgt tatagtgcgt
     2701 cacaaaaaag tagggcgtct gttgctcttt ggtgctgtag cggccgggaa tcacgccttc
     2761 gagcctggac gggccatatc ccatgtctgg cgctagaagg ggcatcgtag gtttcacaaa
     2821 acgcagtccc acgcccgtg
//
LOCUS       DASZMB010000141         6342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_5082969, whole genome shotgun sequence.
ACCESSION   DASZMB010000141 DASZMB010000000
VERSION     DASZMB010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6342
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5082969"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VG272_06500"
     CDS             <1..252
                     /locus_tag="VG272_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13367.1"
                     /translation="NLKNVSYTDSFEGDKMNPVTEPQLKPNDSKNLQDCKSYAFAFAS
                     NGKAELRIIGEYTREDLEDLKTHLEVTMRGLLRSKGNDA"
     gene            complement(298..483)
                     /locus_tag="VG272_06505"
     CDS             complement(298..483)
                     /locus_tag="VG272_06505"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HWF13368.1"
                     /translation="MTKRMTSNVRVRSLLTDLENDVMQAVWEGGTCSVEAVHLIVSRN
                     RNLKETTVRTLLGSGVI"
     gene            complement(643..2247)
                     /locus_tag="VG272_06510"
     CDS             complement(643..2247)
                     /locus_tag="VG272_06510"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: protein motif:HMM:NF004831.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HWF13369.1"
                     /translation="MTLNAVWPVVVVGAGPNGLTAANLLARYGIDVLLVERNEITVQE
                     PRAVSIDDESLRTMQSIGVVDEVVSRVVLGYGSDYFTARGSCFLRVRPTAREYGYPRR
                     NAFRQPVFEQQLRDYFRTHSSRDTRSESWFSTELIDFHQTEDAVHLVLRRADGSLAEV
                     STPYLVACDGGRSFVREKLGIKLSGSTFRERWLILDLEETRDSSRDTKVFCDPVRPCL
                     SLPGPDRTRRFEFMLQEGEMEPEVTSPEFIRKLLLPHGEENTPLRRSRVYTFHARMAD
                     RWRDRRIFLVGDAAHLTPPFAGQGMNSGIRDAHNLAWKLASVIQGRLGPGLLDTYEME
                     RRKHAWEMILLALRMGKVMMPRSTWSAFGLQAAFRLLGLVPPARNYFAEMKYKPKPHF
                     EEGFLVREKKHSAASLVGRLFPQPRVRYAGRECLLDDFLGNGFALLSLPQTHPRLFEN
                     LRADLWPPLNLQRVAIRESGDIEPSPNGVTCVYEIHGEYSRALTHMPPGLLLIRPDRY
                     VAAFLSADEIDAAVSSVDDMIAQTWK"
     gene            complement(2611..3573)
                     /locus_tag="VG272_06515"
     CDS             complement(2611..3573)
                     /locus_tag="VG272_06515"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWF13370.1"
                     /translation="MADTKDKVTLQDIQTTSERLKNWGRWGADDEIGTLNNISAQDII
                     KAAGLIRKGKVFSLALNYDSNGPQNGLWGNRFNPIHTMLATGTDAVAGRQESIKIRYA
                     DDMVSMPLQCGTQWDALGHVFNGEKMWNGYDARLVDSNGAAKNGIEKVKDKMVGRGVL
                     LDIARHKGVDSLEDGYGISIADLESCAKAQGVEVRKGDFVIFRTGHMERCLKEATWGG
                     YAGGDAPGLRFETADWIRSTDMAAICADTWGCEVRPNETTIASQPWHWVVIPMIGISM
                     GEIFYLKDLAVDCAQDHVYEFFFCAPPLPITKAVGSPVNPIAIK"
     gene            3740..4666
                     /locus_tag="VG272_06520"
     CDS             3740..4666
                     /locus_tag="VG272_06520"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWF13371.1"
                     /translation="MLGQWKGKNMDSKPVGMIGLGIMGSAMSVNLIRAGFKVAGYDVV
                     PRARQVHRKAGGAVARSCQEIAKKCGIIVTSLPSSDALLETAAQLAKSARTNRIVVET
                     STLPIPVKEEARKILASRGTILLDCTLSGTGAQARVKDLIVYASGDRKACNRVAPVME
                     GFARAHYYVGAFGAGCKMKFVANLLVAIHNVAAAEAMVLAMKSGLDPEMVLKVVADGA
                     GGSRMLQVRGPMMAKGDYSDATMKVQTWQKDMTIIGDYARKIDCPTPLFSASAPFYTA
                     AMAMGMGAEDTGAVCAVLERMANNPRETSGKK"
     gene            complement(4759..6135)
                     /locus_tag="VG272_06525"
     CDS             complement(4759..6135)
                     /locus_tag="VG272_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF13372.1"
                     /translation="MLQFLEQQEELTSNQRKIIAAAVLGDMLEFFDYFLIGYVVAFIA
                     GPWKLTFGESSVILLSSGFGAMFGAVFYGWLADKIGRRKVFLMTVINFSIATGILTFT
                     PDNAWVFLSIFRFLIGFGVGGLYCVDLPLVQEFVPASKRGLVGGLVTAAVPIGVLLGA
                     ALGAYATPYVGWRGLFAIGMVPGLLTLLIRAWVPESPRWLIQMGRMDEARKSLAWALE
                     TDPEKLPLGAAEGQKQPMSKLSDIFKYPRSLAVSWITNLGAQTGYYGLTLWVPTLMVQ
                     LLGVSPARASFLLIFVNFAGLFGRVGLSYLSDSIGRKGAGAIASFGAAILTVSAAIYH
                     SAFVGTFSVFWIILIVNNAFADGGFAIVGPYASEVWPAALRTTGMGSAYGFGGLGKVI
                     GPLGLALIVGSSNIITPKASVDALFPAFSYLAAWFAIAGLAYIFIGKETKGQSLEKIE
                     RDLESSRS"
BASE COUNT         1545 a         1748 c         1709 g         1340 t
ORIGIN      
        1 aacctgaaaa acgtatccta tactgattcg tttgagggag ataaaatgaa ccctgtgacg
       61 gaaccgcaac tcaagcccaa cgattcgaag aacttacaag attgcaagtc ttatgctttt
      121 gcttttgcta gtaacggtaa agcggagtta cgaattatag gtgaatacac tcgcgaagat
      181 ttagaggact tgaaaactca tcttgaggtt actatgcgcg gattattgcg ctcaaagggt
      241 aacgacgcat gagaaactgg cggccctctc tagcgaggcc gccaccaact tctcaaatca
      301 aatgacacca ctacccagca acgtgcgaac ggttgtttct ttgagattgc gattgcggga
      361 cacgatcaga tggacggctt ccacactgca cgttccgcct tcccatacgg cctgcattac
      421 atcgttttct aggtccgtga ggagagaacg aacacgaaca ttggatgtca tgcgctttgt
      481 catggtacat tctgtaccac ttctcaaaat cgtcgtcaag aactttttgg attttgacag
      541 gagagccggc gatggatagg accgcgaggc tacgttcatc ggaaattaga gacgtttatg
      601 aagccaagtc ttgatacctc gtcttatcgg aatatgcaaa aactacttcc acgtctgggc
      661 aatcatgtcg tctacactgc tcactgctgc atctatctca tccgccgaaa gaaacgccgc
      721 tacatagcgg tcggggcgaa tgagcagcag accgggtggc atgtgtgtca gcgcgcgcga
      781 atattctcca tggatttcgt atacacaggt gactccgttc ggcgaaggtt cgatatcgcc
      841 ggactcgcgg attgcgacgc gctgcagatt gagcggtggc cacagatcgg cgcgcagatt
      901 ttcaaacaaa cgcggatgag tctgcggcag agagagcagc gcaaatccat ttcccagaaa
      961 atcatcgagc aggcattccc tgccggcgta gcgaacccgc ggctgcggaa atagcctgcc
     1021 aacaagggat gccgcggaat gctttttttc gcggaccagg aacccttctt caaagtgcgg
     1081 cttcggtttg tatttcattt ccgcaaaata attacgcgcc ggcgggacaa gaccgagcaa
     1141 ccgaaacgcg gcctgcaaac cgaacgcgct ccatgtgctg cggggcatca tcactttgcc
     1201 catgcgcagc gccagcaaaa tcatttccca ggcatgcttg cgccgctcca tttcgtaagt
     1261 gtccagcaaa cccgggccga ggcggccttg aatcacggac gcgagtttcc aggcgagatt
     1321 gtgcgcgtcg cggataccgc tattcattcc ctgcccggca aacggcggtg tgaggtgcgc
     1381 ggcatcgccg acgagaaaaa tgcggcggtc cctccacctg tccgccatgc gcgcgtgaaa
     1441 cgtatacaca cggctgcgtc gcagcggcgt gttctcttcg ccgtgtggca ataacaattt
     1501 tcggatgaac tcgggagacg ttacctcagg ctccatctcg ccttcctgca gcatgaattc
     1561 aaaacggcgc gtgcggtccg gtccgggaag cgagaggcac gggcgcaccg gatcgcaaaa
     1621 aacttttgtg tcacgcgagg aatcacgcgt ttcttccaga tcgaggatga gccagcgttc
     1681 ccgaaacgtg gaaccgctga gtttgatccc aagtttttcg cggacgaagc tgcggcctcc
     1741 gtcgcacgcg acgagataag gcgtggaaac ctcggcgagg cttccatccg cgcgacgcaa
     1801 gacgagatgc accgcatcct cggtttggtg gaagtcaatt aattccgtgc tgaaccacga
     1861 ttcggaccga gtgtccctcg acgaatgcgt acggaaataa tcgcgaagct gctgttcaaa
     1921 aacaggctgg cgaaatgcgt tgcggcgcgg gtagccatat tccctggcgg ttggacgcac
     1981 gcgcagaaaa caagatccac gcgcagtgaa ataatcggat ccatacccaa gcacgacgcg
     2041 cgacacgact tcatcgacga cgccgatcga ttgcatggtg cgcaacgatt catcgtcaat
     2101 ggaaacggcg cgcggctctt gaaccgtaat ctcgttgcgc tcgacgagga gcacatcgat
     2161 gccgtagcga gccaaaagat tcgcggcagt gagcccattg ggccctgctc ctacgacaac
     2221 aacaggccac accgcattta atgtcatatg ctaacgcgca agaatctgga agccatccca
     2281 taaatggctc gccgcagata cttaatagga cttcaggggc taaagcccct gattttcatg
     2341 tccctaaatg tcgcggctaa agccgcgacc cacaaagacc gaacgtcaag actggcccac
     2401 aaagagtgac tctcaaaaaa ctgacctacg acttaagcgt gaagactatt catgagatga
     2461 gataagttag acaggttcta gaaaccttct cgtaaatgat ctttgtgggt cacggcttca
     2521 gccgtgacat tcaagaattt gaaaagcaag ggctttagcc cctgatggcc gatcgagata
     2581 tcttacgtcc atttatgaga cggtttcctg tcacttgatc gcgatggggt tcacaggcga
     2641 tcctactgct tttgtgattg gcaggggcgg cgcgcagaaa aagaattcgt atacatgatc
     2701 ttgcgcgcaa tccacggcca aatctttgag ataaaaaatt tcgcccatgc tgatgccgat
     2761 catggggatg actacccagt gccacggctg gctcgcgata gtcgtctcgt ttggccggac
     2821 ttcgcagccc cacgtatccg cgcagatcgc ggccatgtcc gtgctgcgaa tccagtcggc
     2881 ggtttcaaac cgcagcccag gcgcatcgcc acccgcgtaa cctccccatg ttgcttcctt
     2941 caggcagcgc tccatgtgtc cggtgcgaaa aattacaaaa tcgcccttgc ggacttccac
     3001 gccttgcgct ttggcgcagc tttcgagatc ggcgatgctg atgccgtaac catcttcgag
     3061 cgaatcgaca cccttgtgcc gcgcgatgtc caggagcaca ccgcgtccga ccattttgtc
     3121 tttcactttc tcgatgccgt tcttcgccgc gccgttggaa tcgacaagcc gcgcgtcgta
     3181 gccgttccac attttttcgc cgttgaatac gtggcctagc gcgtcccatt gcgtgccgca
     3241 ctgcagcggc atggaaacca tgtcatcggc gtagcgaatc ttgatggatt cttgccgccc
     3301 ggctaccgcg tcggttcccg tggcgagcat cgtgtgaatg ggattaaagc gattgcccca
     3361 gaggccgttc tgcggtccat tgctgtcata attgagcgcc agcgaaaata cttttccttt
     3421 acggatgagg cccgctgctt taatgatgtc ttgcgcggaa atattattca gtgttccgat
     3481 ttcgtcgtca gcaccccagc gcccccaatt cttcaatcgt tccgacgtcg tctgtatgtc
     3541 ctgaagcgta actttatcct tcgtgtcggc catgagcctc aaccttctgt atgtaaaatt
     3601 aaaattgcag cacaaattta tagattataa tcacacggaa ttccacttct ttgctgcttt
     3661 tccttacttt cacaaaaaat ttgcctaact cttttcgact ggccagctct gtctgctaga
     3721 gtttatcttc acatttactt tgctgggaca atggaaaggg aaaaacatgg attctaaacc
     3781 tgtgggcatg attgggctgg gaattatggg atcggcgatg tcggtcaatc tcattcgtgc
     3841 aggtttcaag gttgctggct acgatgttgt gccgcgtgcg cgtcaagtac accggaaagc
     3901 cggtggagcg gtcgcccgca gttgccagga aatagcgaaa aagtgcggaa tcatcgtgac
     3961 ctcgctgcca tcttccgatg ctctgctcga aactgccgca cagcttgcaa agtccgcgcg
     4021 cacgaatcga atcgtcgttg agaccagcac gctgccaatc ccggtgaaag aagaagcgcg
     4081 aaaaatcctc gcatcgcgtg gcacgatttt gctggattgc acgctgagcg ggacaggcgc
     4141 gcaagcgcgg gtgaaagact tgatcgtgta cgccagcgga gaccgcaagg cgtgcaatcg
     4201 cgtcgctccg gtcatggagg gttttgcgcg cgcgcattac tacgtcggcg cttttggcgc
     4261 gggctgcaaa atgaagtttg tcgcaaatct tctggtggcg attcacaacg tcgcggcggc
     4321 tgaagcgatg gtcctggcca tgaagtcggg actcgatccg gagatggtat taaaagttgt
     4381 ggcggatggc gcgggaggct cgcgaatgct tcaagtacgc ggcccgatga tggccaaagg
     4441 ggattactcc gatgcgacga tgaaagtgca aacctggcag aaagatatga ccatcattgg
     4501 agactacgcg cgcaagatcg attgccccac gcctctgttt tcggccagcg cccctttcta
     4561 cacagcagcc atggcgatgg gaatgggcgc ggaagataca ggagcggtgt gcgccgtact
     4621 cgagcggatg gccaataacc cgcgcgagac cagcgggaaa aaatagcggg ggtaggttgg
     4681 attgtcggaa cagaaaaacc cgccaaaccc gctgtagccc gggtcttcag acccgggggt
     4741 ttagatcaca ccttgccgct aactgcgtga actttcgagg tcccgctcaa tcttctccag
     4801 cgactggcct ttcgtttcct tgccgatgaa aatataagcg aggccagcga tggcaaacca
     4861 tgccgccaga tagctgaacg ccgggaacaa tgcatccacc gatgccttcg gcgtgatgat
     4921 gttcgaagat cccacgatca gcgccaaccc cagcggaccg atcactttcc caagaccgcc
     4981 gaaaccatag gctgaaccca ttccggtggt tcgtagtgcc gcgggccaaa cttctgacgc
     5041 gtacgggccc acgatcgcaa agcctccgtc cgcgaaagca ttgtttacaa tcaaaatgat
     5101 ccaaaacacg gagaatgtcc ctacgaaggc actgtggtag atcgcagccg aaacggtcag
     5161 gattgccgcg ccaaagctgg caatggctcc ggcacccttg cggccaatgg aatccgacag
     5221 gtaagagagc cccacccgcc cgaatagacc tgcaaaattg acaaagatca gcaggaatga
     5281 cgcccgggcc gggctaacgc ccaaaagctg gaccataagc gtcggaaccc acagagtaag
     5341 cccgtaatag cctgtctgcg cccccaaatt ggtgatccag gagactgcca ggctccgcgg
     5401 gtacttaaag atgtccgaca gctttgacat gggttgcttc tgcccctcag cggcacccaa
     5461 gggaagcttc tcagggtccg tttccaacgc ccatgccaag gatttgcggg cttcgtccat
     5521 acggcccatt tggatcagcc agcggggcga ttccggcacc caggcgcgga tcagcaacgt
     5581 cagcagcccg ggaaccatgc caattgcgaa taaacccctc caaccaacat agggggtggc
     5641 ataagcgccc aacgcggctc ccaagaggac accaataggc acggctgcgg tgaccaggcc
     5701 gccgaccaag ccccgtttag aggccggcac gaactcctga accaggggaa ggtcaacgca
     5761 atacaagcct ccgacaccga atccgataag gaagcgaaaa atggagagga aaacccaagc
     5821 attgtcgggt gtaaaggtca gaatgcccgt ggcgatcgag aagttgatca ccgtcatgag
     5881 gaaaacttta cggcggccaa tcttgtccgc gagccagcca taaaatacgg ctccgaacat
     5941 cgcgccaaat cctgaagaca aaaggattac ggacgattcg ccgaatgtaa gcttccatgg
     6001 accggcgatg aacgcgacta cgtaaccgat gagaaaatag tcgaagaatt caagcatgtc
     6061 gcccagtacg gcggcggcaa taattttacg ctgattgctg gttagctctt cttgctgctc
     6121 aagaaattgg agcatatctg gttttggccc ggcgaatcat acaccatagg ggagagagag
     6181 gcacagccct gagaaggata gtgtcttagc tccataatta tgtcacccct ttatggctac
     6241 gtgaaaatgt caccccatga gacaggggac atttgcattg agccaaaaag aattacaacg
     6301 agtagcagtc atcacatctt gcgtgaaggg ggacctggcg tg
//
LOCUS       DASZMB010000142         3705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3991435, whole genome shotgun sequence.
ACCESSION   DASZMB010000142 DASZMB010000000
VERSION     DASZMB010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3991435"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(357..1307)
                     /locus_tag="VG272_06530"
     CDS             complement(357..1307)
                     /locus_tag="VG272_06530"
                     /inference="COORDINATES: protein motif:HMM:NF040043.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HWF13373.1"
                     /translation="MTSMTPGGGEGAAGAKVTPVTDSAWKCLQGAYDMQVHVAPDVIE
                     RRIDDLDLAKEFLGRGLKGFILKSHYFPTAERAKVVTKAVPGIAAYGAITLNHSVGGL
                     NPVALELAGRSGCKIVWFPTVDANNETAGRLDGPNVKLPFWAKIQRELAATGIAPPPL
                     TVLDLDGKTSPVTLRCIEIIAKHNMILATGHLGRVEIFELVKSARAMGTQKVLVTHAE
                     FPSQNLTADEQKHLADMGAFIEHCFTTMHTGKAPWEAVMEAIRVVGPERCVLSTDLGQ
                     TINPPVAEGFAMFTQKMLDAGFSVDQIRHMTVTNPSALLA"
     gene            complement(1542..2252)
                     /locus_tag="VG272_06535"
     CDS             complement(1542..2252)
                     /locus_tag="VG272_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13374.1"
                     /translation="MNKRRTLSYFFLVGSIFLFAGILFAQSKSNRALIVNGKQQGSVV
                     QLNGHAYIDVETVAQFMNGSVTLETNRVVLNFPEPQPVEAVATPAPETQQPAAQPVPA
                     GLLTRNFASLAISELAEMREWRGAVGTILTYGVPVVGTWPQDYHDRVQANLDQVSVAA
                     STPGDEDAMRLLQTEFSNVARWTNDVVSARQSLNATNSVRPDALQSDPMLAKIANCSR
                     FLSSMLVSGTFADDASCH"
     gene            complement(2549..3532)
                     /locus_tag="VG272_06540"
     CDS             complement(2549..3532)
                     /locus_tag="VG272_06540"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWF13375.1"
                     /translation="MADSGASTRKLKMADIHAAAERLKNWGKWGPDDQIGTLNYVQPE
                     DIVAATRLVQRGKVISLALNFDSNGPQGGKSKYPAMGRFNPIHLMLRTGTDAYSGVLD
                     SRKIRGSDDIIMMPTQAGTQWDGLGHIFYGDHMWNGYDCRTVTSAGAQKCGIEKTKDR
                     MVGRGVLFDIPRMLGMQYLPDTFAITNEMLDKAEKHFGVEVRRGDYLLVRTGQMEAML
                     SAKSWDGYSGGDAPGLAFETLDWLHKKQVAGIATDTWGAEVRPNESDDANQPWHWISI
                     PIMGLTVGEIFDLAELAKECADDKKYEFLFVAPALPVTGAVASPVNPLAIK"
BASE COUNT          790 a         1078 c          962 g          875 t
ORIGIN      
        1 ttcttggctt cattgaaaat ttctccgtcg tcgattgaat ttcgccagcg tccaacctga
       61 aagctaattc ccaaatcatt tttgcgggtc gcggcttcac aggttgcgga aaaacaaaaa
      121 cgtgtcaatc cgaggcctgc agcttgacga ggaatctctt tcttttcggc ttaacatcga
      181 acagagattc tcactacgtt cggaatgacc accttcgtcg agcagcacag ccgctcttgg
      241 cagagtgcag tttgcaaaac ccgcacgtgc aagagtgcct gtgctactca accatcagcc
      301 caaacccttt tttccgcggc ccgtcgagcc gcgaccgtca gaggacatca aagaactcac
      361 gcaagcagcg cggacggatt cgtgaccgtc atatgccgga tttggtcgac gctgaaaccc
      421 gcatcgagca ttttctgcgt gaacatcgcg aatccctcgg caacaggcgg attaatcgtc
      481 tggcctaaat ccgtcgaaag cacgcagcgc tccggcccaa caacgcgaat cgcttccatc
      541 accgcttccc acggagcttt tccggtgtgc atggtcgtaa agcagtgctc gatgaacgcg
      601 cccatgtcgg cgagatgctt ttgttcgtcc gccgtcaaat tttgcgatgg aaattcggcg
      661 tgcgtcacca aaactttttg cgtccccatt gcccgcgcgc tcttcactag ctcaaaaatt
      721 tccacgcgcc cgaggtgccc ggtggccagg atcatattgt gcttggcgat gatttcgatg
      781 cagcgcagtg ttacgggaga agtttttccg tcgaggtcca gcacggtaag tggcggcggc
      841 gcgattcctg tagcggcgag ctcacgctga atttttgccc aaaacggaag cttcacgttc
      901 ggaccatcga gccgccccgc agtttcgttg tttgcatcca ctgtcggaaa ccatacaatt
      961 ttgcagccag atcgccccgc aagctccagc gccacgggat tcaatccgcc cacagagtgg
     1021 tttagcgtaa ttgctccgta cgcggcgatg cccggcacgg ctttcgttac gactttcgcg
     1081 cgttcggccg ttgggaaata atgcgacttc agaataaatc ctttgagccc gcgccccaga
     1141 aattccttgg ccaaatccag atcgtcaatg cgtcgctcaa tcacgtccgg cgcaacatgc
     1201 acctgcatat cgtacgcgcc ctgcaaacat ttccatgcgg agtccgtgac cggcgtaact
     1261 ttcgctccag ccgcgccttc tccgccgcca ggcgtcattg aagtcatggg ttggtccttt
     1321 ccattcaata agttctgttt cgttcatgaa attgcgtgca aatttaagcg aatgcctaag
     1381 aattattcat gggagtcgca gcgacagaat ataccccatc gtcgacacgt ttcactatag
     1441 gggtttgagt cgcacaggct tcagcctgtg gggtttatgc catgcaaggt caaagttccg
     1501 cagattttga agcctgtact acttaaaccg gcgccgaaga attaatggca gctggcatcg
     1561 tccgcgaacg ttccgctaac gagcatcgag ctgagaaacc tgctgcaatt ggcaattttg
     1621 gcaagcatgg ggtcgctctg gagcgcatcg gggcgcacgg aattggtggc gttcagcgat
     1681 tggcgcgccg acaccacatc attcgtccaa cgcgccacat ttgaaaactc ggtttgcagg
     1741 agacgcatcg catcctcatc gccgggagtg gacgccgcga cgctcacctg atcgagattg
     1801 gcctgcacgc gatcgtgata atcctgcggc cacgttccta ccactggcac tccatacgtg
     1861 agaatcgtgc ccactgcgcc gcgccattcg cgcatctccg cgagttcgct gatggccaga
     1921 ctcgcaaaat ttctggtgag caatcccgct ggaaccggtt gtgccgcggg ctgctgagtt
     1981 tccggcgcag gcgtcgccac agcctcaacg ggctgaggct ctggaaaatt aagcactacg
     2041 cgattcgtct ccagcgttac ggaaccattc atgaattgcg ccacggtctc cacgtcgatg
     2101 taggcgtggc cattcagctg taccacgctg ccctgctgtt tcccatttac gatcaaagcg
     2161 cgattgcttt tactttgcgc aaataaaatt ccggcgaaca ggaaaatcga tccgacgagg
     2221 aaaaaataag agagtgtcct gcgcttgttc atgatgaccc cgcttccatg cctatcgggc
     2281 ttcggacaac cgccaataca taggttacag cgattgcgag tgggcttcta gggggagttc
     2341 aacttagagg cggtgggaaa aatgcggagg aaaagcggaa ttccacgatg aaatggtcta
     2401 ctcgtccgtt ccactgtaaa ggctttaaca cagagaacac cgagaaaaac aagagaagga
     2461 ggagagatgc ttcgatcgca ctttcagcct cccagccttt ctcgtttttt tctgtgtccg
     2521 ctgtgttaag tctttgcggg gttttatttc acttgatcgc cagcggattc accggcgacg
     2581 caaccgcgcc cgtaaccggc aatgccggcg ccacaaaaag aaattcgtac ttcttgtcgt
     2641 ccgcgcattc tttcgccagc tccgcgaggt cgaagatttc gcctacggtg agccccatga
     2701 tggggatgga aatccaatgc cacggctggt tcgcgtcgtc cgattcgttg ggccgaactt
     2761 ccgcgcccca cgtatccgtg gcaatgcccg cgacttgctt cttgtgcagc cagtccaggg
     2821 tttcaaacgc gaggcccggg gcgtcgccac ccgagtagcc gtcccacgac tttgcggaaa
     2881 gcatcgcttc catctgtccg gtgcgaacca gcagataatc gccgcgccgc acttccactc
     2941 caaaatgttt ttccgccttg tccagcattt cgtttgtgat ggcaaatgtg tccgggagat
     3001 attgcattcc gagcatgcgc ggaatatcga agagcacgcc gcggccgacc atgcgatcct
     3061 tggttttctc gatgccgcat ttctgcgcgc ccgccgacgt caccgtgcgg caatcatatc
     3121 cattccacat atgatctccg taaaaaatgt ggcccagccc atcccactgc gtgccggcct
     3181 gtgttggcat catgatgatg tcgtcggacc cgcgaatttt gcggctgtcc agcacgccgg
     3241 aatacgcgtc cgttccggtg cgcaacatca aatggatcgg attgaatcgg cccatggccg
     3301 gatatttcga cttgccgccc tgaggtccgt tgctgtcaaa attcagcgcc agggaaatca
     3361 ctttgccgcg ttgcacgagc cgcgtcgctg cgacgatgtc ttccggctgc acatagttca
     3421 gcgttccgat ttgatcgtct gggccccatt tgccccagtt tttcagccgc tccgccgccg
     3481 catgtatatc ggccatcttc aatttgcgcg ttgatgctcc cgagtctgcc atccgtcctc
     3541 caatgtcgtt gattagtagg gataggaata cacgaatcta taatttcagc ccgtcattgt
     3601 actggaatta aaagcgaggt gtgtgcggat ttatgtggca taggcttcag cctgtgcggt
     3661 tttagtcctt gcaagaccta actgcgcaca ggctgaagcc tatgc
//
LOCUS       DASZMB010000143        14556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_132815, whole genome shotgun sequence.
ACCESSION   DASZMB010000143 DASZMB010000000
VERSION     DASZMB010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 14556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14556
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132815"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /locus_tag="VG272_06545"
     CDS             complement(<1..774)
                     /locus_tag="VG272_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HWF13376.1"
                     /translation="MDITVDTRAIAKIEADALVTYAFDQEKPTEGILAQLDAATGGAL
                     TKLATSGELTGKMLETTLLYYPAGLAAQRLLVVGAGKKAKFGTAELRRLAGAAVRALK
                     SRQVKNVAFLAREDDRTAAAAQAIVEGMILANFDSDKYKTDKKPGNEIISAALAGWEE
                     AARADADLGLSRGRIIAESQNFARELGNEPSNKLTPRMLADRAAAMAREAGLSVEVFD
                     ENKIAELKMGALLSVAQGSAEPPRMIIMTYIPATVKEGAP"
     gene            complement(1101..1592)
                     /gene="smpB"
                     /locus_tag="VG272_06550"
     CDS             complement(1101..1592)
                     /gene="smpB"
                     /locus_tag="VG272_06550"
                     /inference="COORDINATES: protein motif:HMM:NF003843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HWF13377.1"
                     /translation="MPKPPKKQEKKVEEVLVAQNRAASYNYHLLERLEAGMVLLGTEV
                     KSLRESKANLRDAYGLIRKGEVWLVNFHIGEYLPGGPFNHGPLRTRKLLLRKEQIAKL
                     ERKLDDKGLTLIPLRIYFRDGIAKCEIALAQGKKKWDRRQTEKDKESKREVGEAMRKF
                     SRR"
     gene            1702..3966
                     /locus_tag="VG272_06555"
     CDS             1702..3966
                     /locus_tag="VG272_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HWF13378.1"
                     /translation="MIYRKLSGLVLISTAVLLSGCPKSHQDNNAGAKAEAVKDYDTAL
                     DYYNKALQASPNNTEYKLKAARARFEASQWHVDQGRRFRQDSNLELALAEFRKAQMID
                     PSSAVAQQEVKATTDAINAKNGITQDNAPSGRPASEPKLMTGPPQIQPMSRAPINMRA
                     TNDSKAVFDAIGKLAGLTVIFDPDFTSRRISVELTNVTLEQALNITALQSKAFWRPET
                     ANIIFVAPDQPQKRKDYEEEVVKTFYLKNTVLPQELTEIVTSIRQLLDLRRVQQINAQ
                     NAIVIRDTPDRVMLAGKIIDDIDKAKPEVVIQVAVMEARRDRMRNIGITPPTSVSATF
                     ANPGTTSTTSSSTSTTTTNPAATISQLVSDPHIFSNGYSVTVPGASASFLMTDTSTQI
                     LDDPEIRVVDGQTAKLRIGDRIPVATGSFQAGIGVGGTGTGGINPLVNTQFQYLDVGV
                     NVDVTPRIHPDHEISMKINVVVSSQTGSTNIGGINQPIISQRSIEHDIRLKDGEVNVL
                     GGLMEQTDTDSINGIPGLAKIPFFKNFFSGNVVDHEKDEILIVLIPHIVRLPSISAEN
                     LRSISSGTDTNAEVRLEDAVLLPDVVKPQAAIVPQAAPAAPPATTNTSAAQNATLRFD
                     PATAALKVGETTTIGVAVQNVQDLYSIPMLLQFNPAVISVEDVRQGGFLSGGTQEIAI
                     VERVDKERGQAIISATRMPNTPGVSGSGTIVGVVVKAIAPGNSQLSIVQVNAKDSQQR
                     PIQVVTGEATVKVQ"
     gene            3985..4653
                     /locus_tag="VG272_06560"
     CDS             3985..4653
                     /locus_tag="VG272_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13379.1"
                     /translation="MRQPNSVPRTARQKDAGLTLLELILTIGILLVLSTAAIPMFRVT
                     IQHRKETELRNDLREMRNSIDRYKDDADKNLIRTEVGSQNYPPDLQTLVDGVEVSNAG
                     GGAGGISASALAGASGTSQFGSAGTSQFSTPQFGTGAAGGTSQQSSGLASGLGQALST
                     APSKVRYLRKIPVDPMTGKADWGLRAVQDDPDSDSWGGHNVFDVYSQSQAISSDEKTK
                     YSDW"
     gene            4661..5116
                     /locus_tag="VG272_06565"
     CDS             4661..5116
                     /locus_tag="VG272_06565"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HWF13380.1"
                     /translation="MNSSHISACDCKNLQAPGSPARRHLLTAGFTLLEAMIVVTIILI
                     LAGMAAVRYEKSVLRAREATLKQDLFIMRNAIQQYTLDKEAGPSSLDDLVPKYISGIP
                     TDPITRSKNWHTDSDPVLLDPQQTSPGITDVHSASDQSSPIENTPYNTW"
     gene            complement(5177..6310)
                     /locus_tag="VG272_06570"
     CDS             complement(5177..6310)
                     /locus_tag="VG272_06570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWF13381.1"
                     /translation="MPREVSHPQWGKPPWSIQFQPSRPAMPREADFAVVGAGFSGLSA
                     ATWLRRFAPDKTVVVFESASIGAGASGHTGGMVLAETAAGDLPGLGDVLGGFSSILKD
                     LDIECDLALPGVFEIARQGGLPGSPISWSDSGIVRVVNNVPGGMVDPGKMVSGLACAA
                     EKSGAQLFENLPVEEIEFADIPCLHFSGGELRARKILFTTNSMSLEMSELAHRGQPKF
                     TLAAATEPLTAGQWSGLGLSSYKPFYTVDFPYLWGRMLATGGCVFGCGLVSVENWREI
                     ENLNINAGQSAELISRLESRVRALHPVMKNVRFANRWGGPILIANEWRPVFARHPRSA
                     HAVVLGAFSGHGVAQSVYLGRWAAEVLCDRKILPSWNARKESA"
     gene            complement(6418..9018)
                     /locus_tag="VG272_06575"
     CDS             complement(6418..9018)
                     /locus_tag="VG272_06575"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWF13382.1"
                     /translation="MSILRSRPNVFRWIVLGALFLSVSLCEARGFAREPDTDSPPQAT
                     TASISGRVSVASSQGVSNNLSSITVILTGPLPATTVQNQVSDAEGRFEFSRLVQGNYK
                     LDVTVEGFKPWSSAVTLTTGQALVQDIGLQLNLVEENVEVKGEATEIETESATPNTTV
                     SEQQLETLPLRTGKFTEALSVSPSVIKTQEGRLNFNGQAESQGMLLVDSAENVDPVAG
                     SFAIPVPVDAIQSIHVFNTPDSVAYGGFSAGLTRIDLKPPSPSWNYKILDFLPSFRGK
                     NDHLVGIANITPRVEVGGPLIKDKLNFSQDVSYEFRRDPIRGLTWPFNETIVYAFDSF
                     TNLQWILSPKHLVDINLNLFQSTNQFANIDALIPQSASVNFRRRGGSLGITDAYQFDS
                     GMVLNTVFRYTNFYTDDHGQGPEQMTINPEGWGGNYFNTSSRNADQVEVLPVLQLPMK
                     SWFGQHEIRFGVDFLYRTFTGNSLSRNINLLNQGATLNQDGTTAEQISFTGPTALRGS
                     DTETSEYVEDHWALTKALALTLGIRLSSQTIGLDAAFAPRAGIAYSIPRWNTVLRGGA
                     GLIYSHVPLLAQDFADNQTRQLAFLTGPDAGQNFTLQNVYLPGGSLANITSPEQPGNS
                     PRTFTWNIESETAIRRNVTLRVGYYETHTADLFLVNPILPSSPTGNAYLALENTGSAH
                     YRQASATARYSPNEKMEANASYSWSRARGDLNTLSDTFIPFQVPVIRANEYGVQPSDI
                     PNRLLVWGYVHLPFWSLVLSPVADMHSGFAYSNVDVFQNYVGAPNSLRFPTYFSLDVK
                     VYRDFPIRIPFKERPTGKIRKIRIGVYSLDVTNRQNPHDVYNNIASPIFGQFAGFQRR
                     FTGLALGLGE"
     gene            9461..10474
                     /locus_tag="VG272_06580"
     CDS             9461..10474
                     /locus_tag="VG272_06580"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF13383.1"
                     /translation="MKIDIFCHIVPQNYYDRMLKLPETGTTIKRRTANIPAMVSLDLR
                     FRMMDRFGDYCQVVSMAAPPIEAMGDPKLSPELAQLANDGLAELVVKHPDRFPGFVAA
                     LPMNNVGAAVEEIDRAVLKLGAAGVQIYSNVNGRPLDDPEFLPIFEKLAERNVPMWLH
                     PSRSSAFADYATEKKSKFELWWVFGWPYETSVAMARILFAGYFDRFPNLKIISHHLGA
                     MIPYFSGRTGPGLDQLGARTEEEDLALVGRKLQKRPQEYFKMFYADTATFGSLPALRC
                     GLEFFGADQVLFASDSPFDPEKGPGYIRETIRCVDGVEMSDADRYKIYEGNARKLMRL
                     KLA"
     gene            complement(10646..11983)
                     /locus_tag="VG272_06585"
     CDS             complement(10646..11983)
                     /locus_tag="VG272_06585"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF13384.1"
                     /translation="MSTALSKFRRLSPLDWVICGVACIGFAFDTYELLVLTLTVQPAL
                     SEFLSAKAGSAQLNHWIGLMFYVPAIAGGIFGLLGGYLIDRFGRRRVLFWSILLFCFS
                     ALATAYSSSAMQLLVYRCITFVGVSVEFVAATAWLAELFPERDQRETILGFTQVFAST
                     GGILMSGASYLSLSIGRLLPAIHGGHEAWRYTILWGILPAIPLLIVRPFLPESPIWQK
                     KREEGTLQRPSILELFHPEFRKTALLSCFMMACAYAASFGMLQHFARIIPGAPEVKLL
                     AHADQQKIVSALQGVQEVGSLFGRILMAGLAIFILSRKRLLHIFQIPGLILIPLVVLL
                     PAMRDTAMPSWGIFVLGTVSVAQFSFWGNYLPTLYPTHLRGTGESFAANVGGRMLGTS
                     AALITTMIVAYMPGGSVTRQLGYAAGIVGFLAYAVGFIGSFWLPEPPEKRITE"
     gene            12310..13212
                     /locus_tag="VG272_06590"
     CDS             12310..13212
                     /locus_tag="VG272_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13385.1"
                     /translation="MADQPHQIQNRRQPATILLLVSYLVFFAMPQKAAAQGSAGNKPP
                     VENKMPQDTIDSSAQETKLGPPTVPVEQIIKQFAAKEAEFKIERDNFTYTQTFVIQTL
                     DDSNRVDGEYRITSDIVFDERGKRVEKVTNAPTPTLERIMLSKEDLSDLENLYPFVLT
                     SDDIGKYDVNYVDHVQLDELTTYVFDVAPKTLEKNQRYFQGRIWVDDKDFQIVKTFGK
                     PVYAKSRSTENQAFPRFETFRENIEGKYWFPTYTRANEMLRFKGGDDVHIKLTVRYEN
                     YKRFGVTVRIGAPVKPAEPNQEKK"
     gene            complement(13231..>14556)
                     /locus_tag="VG272_06595"
     CDS             complement(13231..>14556)
                     /locus_tag="VG272_06595"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HWF13386.1"
                     /translation="SKADFTLRIAPVTVELTPTRIISTIGYNGTSPGPVLRMKEGKPV
                     TVDVINETDVPELVHWHGLFLPGEVDGVEEENTPMVPPRGRRRYQFTPRPAGTRWYHT
                     HVMAGSDLHRGAYTGQFGFLMIESANNPGNYDQEVFLALREWEPFFNPEEEDDDEDPS
                     NAGPQPERPKTLDTNPSGLEVGYQMFSINDKAMGAGEPIRVKPGQRVLIHLLNASASM
                     NRRIALPGHRFQIVSLDGNAVPTSQSVDVIDIGPGERVDAIVEMNQPGIWVLGTTTDD
                     ARMGGMGVIVEYANQHKQPQWTAPVKAVWDYTIFGRAGTQPAPDSTIDMIFQKIPSGA
                     GLFNSWTVNGKEYPHDQEFVLKQGSRYRLVFRNRSEDAHPLHMHRHSFELVEINGKAT
                     AAIIKDTVVVPGYGRVSVDMVADQPGLTLFHCHIQQHMDYGFKALFRYA"
BASE COUNT         3521 a         3952 c         3885 g         3198 t
ORIGIN      
        1 tggcgcgcct tccttaactg tggcggggat gtatgtcatg atgatcatgc gcggcggctc
       61 agcgcttccc tgcgcaacgg acaaaagcgc ccccattttc aattccgcta ttttattttc
      121 gtcgaaaacc tccacggaca atcctgcttc gcgcgccatg gctgcggctc ggtcggccag
      181 catgcgcggg gtcaacttat ttgagggctc attgcccagt tcgcgcgcga aattctgcga
      241 ttcggcaatg atgcgccctc gcgagaggcc gaggtcggca tcggcgcgcg cagcttcttc
      301 ccaaccagcg agtgcagcag aaataatttc gttccccggc ttcttgtcgg tcttgtactt
      361 gtcggagtca aaattggcga ggatcatgcc ctcgacaatg gcttgcgcgg ccgctgcggt
      421 gcgatcgtct tcgcgcgcga ggaacgcaac gttcttcacc tggcgcgatt tcaacgcgcg
      481 caccgccgcg cctgcaagtc tgcgaagctc ggccgtaccg aacttggcct tcttccctgc
      541 tcctacaacc agcaaacgtt gggctgcgag ccccgcagga taataaagca gcgtggtctc
      601 gagcattttt ccggtcagct caccggatgt ggcaagcttg gtcagcgcac cgcccgtcgc
      661 tgcgtcaagt tgcgcaagaa tgccttctgt gggtttttcc tgatcgaacg catatgtgac
      721 cagcgcatcc gcttcgattt tggcaatcgc acgagtatcg acagtaatgt ccataaatcc
      781 ccttttgtgc tgaaatcaaa actcattctc aaaaatcgcg cgttaaaatc aaattttgaa
      841 atattccgcg cccatctcgc gccgtctagc gcccgccttc aggcgggcat tgggctttag
      901 ccggatgcct cctgaaagcg agcgctgcag gaagcacgaa aaattcaaat gcttgaaaca
      961 catccgtaac ctgaatcgcg tccattactt cttgccggcc agtaaacatt tctgcgcctt
     1021 tcgggttcag tcgtgcgcag ttcgggcaat tctcttcatg tctcgaagca ggcttatcga
     1081 tacggcttga tggcccgccg ctaccggcgg ctgaacttgc gcatggcctc gccgacctct
     1141 cgcttggatt ctttatcttt ttccgtctgg cggcggtccc atttcttttt gccctgcgcg
     1201 agcgcgattt cgcatttggc gatgccgtcg cgaaaataaa tgcgcaacgg gatgagcgtc
     1261 aagcctttat cgtccagctt gcgttccagc ttcgcgattt gttccttgcg aaggagaagt
     1321 tttcgcgttc gcagcgggcc atggttaaat ggaccgccgg gaagatattc gccgatatga
     1381 aaattgacca gccaaacttc gcccttgcgg atcaggccat aggcgtcgcg caggttggcc
     1441 ttactttcgc gcaaggattt cacctcggtg ccaagcaaca ccatgcccgc ttccagcctc
     1501 tcaagaaggt ggtaattgta ggaggcggcc cggttttggg ctaccagaac ctcctcgacc
     1561 tttttttcct gttttttggg tggtttcggc acgggaagct atcttagctg cgcgcagtta
     1621 ctagaagcaa gttcgcacct tgttaccctt ttctgcgaat gctagactga ttcgacggaa
     1681 atatgcccta gtggagagtg gatgatttac aggaaactgt ctggactagt gctaatttca
     1741 acagccgtgc tcctatctgg ttgccccaaa agccaccaag ataacaatgc aggggcaaaa
     1801 gccgaggcgg taaaggatta cgacaccgcg ctcgactatt acaacaaagc tctacaagcc
     1861 tctcccaaca atacggaata caaattgaag gctgccagag ccaggtttga ggcttcccag
     1921 tggcatgtgg atcaagggcg gcgattccgg caggattcca acctggagct ggcgctggcg
     1981 gagtttcgca aggcgcagat gatcgatccg tcgagcgcgg tggcgcaaca ggaagtgaaa
     2041 gccacaacgg acgcgatcaa cgccaagaat ggaatcacgc aggacaatgc accgtccggc
     2101 agaccagcgt cagaaccgaa gttgatgaca gggccgccac aaatacagcc gatgtcccgc
     2161 gcgcccatca acatgcgggc cacgaatgac tccaaggcgg tattcgacgc gattggaaaa
     2221 cttgcggggc ttacggtaat cttcgatccg gatttcactt cgcggcgcat ttccgtcgaa
     2281 ctgacaaatg tgacgctgga gcaggcgctg aacattaccg cgctgcagag caaggcgttc
     2341 tggcgcccgg aaacggccaa cattattttt gtcgcgccgg accagccgca aaagcgtaag
     2401 gattatgagg aagaagtcgt aaaaactttt tatctgaaga atacggtttt gccgcaggag
     2461 cttacggaaa ttgtgaccag cattcgccag ttgctcgatt tgcggcgcgt gcagcagatc
     2521 aatgcgcaaa acgcgattgt gattcgtgac acgccggatc gcgtgatgct cgccggaaaa
     2581 attatcgacg acatcgataa ggccaaaccg gaagtggtga ttcaggtagc cgtgatggaa
     2641 gcgcgacgcg accgcatgcg caatatcggt attacgccgc cgaccagcgt ttccgccacg
     2701 tttgcgaatc caggcactac ttccacgacg tcgtcctcga cttcgacaac taccacgaat
     2761 cccgcggcga cgatcagcca actggtttcc gatccgcata ttttctcgaa tggctacagc
     2821 gttacggtgc caggcgcttc cgcatccttc ttgatgacag acacgtcgac gcaaattctg
     2881 gacgatcctg aaattcgcgt cgtggatgga cagaccgcga agctgcgcat cggcgatcga
     2941 atcccagttg cgacaggaag cttccaggcg ggtatcggcg tcggcggaac aggaaccgga
     3001 ggcattaatc cgctggtgaa cacgcaattt caatatctgg acgttggcgt aaacgtggac
     3061 gtcacgccgc gcattcaccc ggaccacgaa atcagcatga agataaacgt ggtggtttcg
     3121 tcgcaaacgg gctccacaaa tattggcgga atcaaccagc cgatcatcag ccagcgcagc
     3181 atcgagcatg acattcgcct gaaagacggt gaggtcaacg tgcttggcgg attgatggag
     3241 cagacggata cggattcaat taacggcatc ccgggcctgg cgaaaattcc atttttcaaa
     3301 aatttctttt caggaaatgt ggttgaccac gaaaaggatg aaattctgat cgtgttgatt
     3361 ccgcacatcg tgcggctgcc ttccatctca gctgagaacc tgcggagcat ttcctccgga
     3421 acggatacga acgccgaagt gcggctggaa gatgcggtac tgttgcctga cgttgttaag
     3481 ccgcaggccg caattgttcc gcaggccgcg cccgctgcgc ctcccgcgac gacgaatact
     3541 tccgctgcgc aaaatgcaac tctgcgattt gatcccgcca ccgcggcttt gaaagtgggc
     3601 gagacgacaa cgattggtgt cgccgtgcag aatgtgcagg atctctactc gattccgatg
     3661 ctattgcaat ttaatccagc ggtaatttcc gttgaagacg tccgccaagg cggattcctt
     3721 tccggcggaa cgcaggaaat tgcgatcgtg gaacgagtgg acaaggagcg cggccaggcg
     3781 atcatctcgg caacacgaat gccgaataca ccgggagtga gcggttcagg cacgattgtc
     3841 ggcgtggtgg tgaaggccat tgctcccggc aattcgcagc tttccatcgt gcaagtgaat
     3901 gcgaaagact cccagcagcg gcccatccag gtggtcacgg gtgaggccac agtgaaagtt
     3961 caataagatc gaggtcagtt ttccttgcgt caaccaaact cagttccgcg cacggcccga
     4021 cagaaggatg cgggactgac tctgctcgaa cttattttga caattggaat tctgctggtt
     4081 ctctccaccg ccgcgattcc catgtttcgc gtgacgatcc agcaccggaa agaaactgag
     4141 ctgcgcaacg atttgcgcga gatgcgaaac tcgattgacc gttacaagga tgacgccgat
     4201 aaaaatttaa ttcgcacgga agtcgggagc cagaactatc ctcccgattt gcaaacgttg
     4261 gtcgatggcg tagaggtcag caatgccggt ggcggcgctg gaggaataag cgcatccgcc
     4321 ctcgccggcg cgagcggcac gagccagttt ggctccgcgg gaacttctca gttcagcact
     4381 ccgcaatttg ggacgggcgc ggcgggtgga acatctcaac agtcctcggg attggcctcg
     4441 ggattgggcc aggctttgtc taccgctccc agcaaggtgc gctacttgcg gaaaattcca
     4501 gtcgatccga tgacaggcaa agccgattgg ggtttgcgcg ccgtacagga cgatccggat
     4561 tcggacagtt ggggcgggca caacgtgttc gacgtgtatt cgcaatccca ggcgatttcg
     4621 tccgacgaaa aaacgaaata ttcagactgg taattcttga atgaattctt cccacatttc
     4681 ggcttgcgac tgcaaaaatt tacaagctcc aggttcgcct gcgcgccgtc atttgctgac
     4741 ggcaggattt acgttattgg aagcgatgat cgtcgtcacg atcatcttga tcctggcggg
     4801 tatggcggcg gtgcgatatg agaaatcggt cctgcgcgcg agagaagcca cgttgaagca
     4861 ggatttattc atcatgcgga atgccattca gcaatatacg ttggacaagg aagctggacc
     4921 gtcctcgctc gacgatcttg ttccgaaata tatttctgga attccgaccg acccgatcac
     4981 acgcagcaag aattggcata cggattccga tcctgtttta ctcgatccgc aacagacttc
     5041 tcccggaatt acggatgtgc attccgcatc cgatcagagt tcaccgatcg aaaatacgcc
     5101 ttacaacacc tggtaagccg aattttctac tgtttgttaa aaaactgaaa ttctggtggg
     5161 aattaaattt tgggcgctaa gcggattctt tgcgcgcatt ccagcttggc agaattttac
     5221 ggtcgcagag aacttcggcg gcccagcggc ccagatacac cgattgcgcg acaccgtgtc
     5281 cgctgaaagc tcccagcacg actgcatgtg cgctgcgagg atgccgcgca aatacaggcc
     5341 gccactcatt cgcgatcaaa atgggccctc cccagcggtt ggcgaagcgc acattcttca
     5401 tcaccggatg cagcgcgcgc actcgcgatt cgagccgtga aatcagttcc gccgattgcc
     5461 ccgcgttgat attcaagttt tcgatttcgc gccaattttc caccgacacg aggccgcagc
     5521 caaaaacgca accgcccgtc gcaagcatcc tgccccacag atatggaaaa tccacggtgt
     5581 agaacggttt ataagaagag agtcccagtc cggaccattg tcccgcagtt aaaggttctg
     5641 ttgcggcagc cagcgtaaat tttggctgcc cgcgatgcgc aagttcgctc atctccagcg
     5701 acattgagtt tgtcgtaaac aaaattttgc gcgcgcgcag ttccccaccg gaaaaatgca
     5761 ggcacggaat atctgcaaat tcgatttcct cgacggggag gttctcaaaa agctgcgcac
     5821 cacttttttc cgccgcacat gccaagccgc ttaccatttt tccgggatcg accattccgc
     5881 caggaacatt gttcaccacg cggacgatgc ctgagtcgga ccacgaaatg ggcgagccag
     5941 gcaatccgcc ttgccgtgca atttcaaaaa cgccaggcag cgccaaatcg cattcaatgt
     6001 ccaaatcttt caggatggaa ctaaatccgc cgagaacatc tccaagacca ggcagatctc
     6061 cggcagccgt ttcggctagc accattccac cggtatgccc acttgcacct gcgccgatcg
     6121 aagcagattc gaacaccacg acggtcttgt ctggggcaaa ccggcgcagc caggtagcgg
     6181 cggagagtcc cgaaaaacct gcaccaacga cggcaaaatc agcttcgcga ggcatggccg
     6241 gacggctagg ttgaaattgg atggaccagg gtggtttacc ccattgagga tgagaaactt
     6301 ctcgtggcat gccgaatctc ctgacctgat caggatagcc cagttcgatt ggcgtggctc
     6361 acccggtatc cattggggaa tccagtagat gccggtaagg aaagatttcc agaagattta
     6421 ttcgcccagc ccgagagcaa ggccggtgaa tcgccgctgg aagcccgcga attgtccaaa
     6481 aatgggagaa gcaatgttgt tgtacacgtc atgcggattt tggcgattgg taacatctag
     6541 cgaataaacg ccgatgcgaa tttttcgtat ctttccagtg ggccgttcct taaacgggat
     6601 tcgaattgga aaatccctat acactttaac gtcgagagaa aaataagttg ggaatcgcag
     6661 actgttgggt gcgcccacat agttttgaaa cacatcgacg ttcgaatacg caaatcctga
     6721 atgcatgtcc gcaaccgggc tcaacaccaa actccaaaac ggcaggtgga catagcccca
     6781 gaccagcaaa cgattgggga tgtccgaagg ctgaactcca tattcgttgg ctcgaataac
     6841 tggaacctgg aatggaataa aagtgtccga gagcgtgttg aggtcgccgc gcgcacggct
     6901 ccaggaatac gaagcgttgg cttccatttt ctcattcggg ctgtaacgtg cagtggcgct
     6961 ggcctgccgg tagtgcgcgg agccagtatt ttccaacgcc agataggcgt tcccggtggg
     7021 actggagggc aaaatcggat tcaccaggaa aaggtcggcg gtatgggttt cgtagtaacc
     7081 gacacgcaga gtaacgtttc tgcggatcgc ggtttcggat tcaatattcc acgtaaaagt
     7141 tcgcggactg ttgccaggtt gttccggact cgtaatattc gcgagagatc cgccgggaag
     7201 atagacgttc tgcaaagtga aattctgacc cgcatcgggt ccagttagaa aggcaagttg
     7261 ccgcgtttga ttatcggcga agtcctgagc aagcagcgga acatggctgt agattaatcc
     7321 tgcgccaccg cgaagcaccg tgttccaacg cggaatggaa taggcgatgc ccgcgcgtgg
     7381 agcaaatgcg gcatcgagcc caatcgtttg actgctgagg cgaatgccta gcgtcagggc
     7441 aagagctttt gtcaaagccc aatgatcttc cacatattcg gacgtttcgg tatccgatcc
     7501 ccgcaacgca gtgggccctg taaaggagat ctgctcagcc gtcgtgccgt cctgattcag
     7561 cgtggcgccc tggttcagaa gattaatgtt cctggagagg ctgtttcccg tgaaggtcct
     7621 atagaggaaa tcaacgccga atcgaatttc atgttggccg aaccacgatt tcatggggag
     7681 ttgaagaacc ggcagaactt cgacctgatc cgcattccgc gaggacgtat tgaaataatt
     7741 tccgccccaa ccctcgggat tgatggtcat ttgttccggg ccctgcccat gatcatcggt
     7801 ataaaaattg gtgtagcgaa aaaccgtgtt caagaccatc cccgaatcaa actggtaggc
     7861 gtcggtaatc cccagcgaac ctccacgacg acgaaaattc accgaagcac tttgcggaat
     7921 cagcgcgtcg atatttgcaa attgattcgt ggactgaaaa agattcagat taatatcaac
     7981 caaatgtttt ggcgaaagaa tccactgcaa attggtgaag gaatcgaagg catacacaat
     8041 tgtttcgttg aatggccagg tgagtccgcg gatcggatct ctgcgaaatt cgtagctcac
     8101 gtcctgtgag aaattcagtt tgtccttgat cagcggtccg ccgacttcca cacgcggagt
     8161 tatgttcgcg atgccgacga gatgatcgtt tttcccgcgg aaagacggca gaaaatcgag
     8221 aatcttgtaa ttccaggacg gcgagggagg ctttaaatcg atacgcgtca gcccagcgga
     8281 aaatccgccg taggccacgc tatccggtgt gttgaacacg tgaatcgact ggatggcatc
     8341 gaccggcact ggaatggcga agcttccggc cacggggtcc acattttcgg cggagtccac
     8401 gagcagcatg ccctggcttt ctgcctgccc gttgaaattt aatctgcctt cttgcgtttt
     8461 gatcacgctc gggctgacgg aaagcgcctc cgtaaatttg cccgtgcgaa gcggcagagt
     8521 ttccagttgc tgctcgctca ccgtcgtatt cggagtcgcg ctctccgtct cgatttccgt
     8581 ggcttcgccc ttgacctcta cgttttcttc gaccagattc aattgcaggc cgatgtcctg
     8641 aaccaaagct tggccggtcg taagcgtcac agccgaggac catggtttga agccctcgac
     8701 ggtcacgtcc aacttgtaat ttccctggac caaacggctg aattcgaagc gtccctcggc
     8761 gtctgaaacc tggttttgca cggtggtagc gggcagcggg cccgtgagga tgacggtgat
     8821 tgaggataag ttgttgctta cgccctgcga ggacgcaacg cttaccctgc cagaaatgga
     8881 tgcggtggta gcctgcggag gcgaatccgt gtcaggttca cgcgcaaatc cgcgggcctc
     8941 gcacaatgag acggagagga ataatgctcc cagaacgatc caacgaaata cgtttggcct
     9001 gcttcgcagg atactcaact acaactcctc ctgatccata tcctttactt gcccgtaatt
     9061 ccccgctgtc ccaatattag atggaaatgt caggcggatg gcacatgaaa aaatccgcgg
     9121 acaaacagta caaccgccat ccatgtttag ccgcaggaat tacaattgac cacactcggc
     9181 ttgctgcgat tggcccagga ttttcaaact tattccaacc ctaacatctt cgcctgtttt
     9241 gagtcatcaa ccaaacatta ggttgtaagc gattcctcaa agaattacca acctgaattc
     9301 agcacgaatt tcagtgcgag ttcgttgaca ccccacgctg ggcttggtat gtttcctgct
     9361 caattcataa agtaaaaacc atcagataaa cgatttgcag agacgattct aattgtttgc
     9421 cttggcgtcg catgattcaa atttgagaaa gcaggacgaa atgaaaatcg acatcttctg
     9481 ccatattgtt ccacaaaatt attacgaccg catgctgaag cttcctgaaa ccggcacaac
     9541 catcaagcga cgcacggcca atattccagc gatggtcagc ctcgatctgc ggttccgcat
     9601 gatggatcgc tttggcgact actgccaggt ggtgagcatg gccgcgccgc caatcgaggc
     9661 catgggcgat ccgaaacttt ctccagagct tgcgcaacta gcgaacgatg gcctggccga
     9721 actggtcgta aagcatcccg accgctttcc gggattcgtg gccgctctgc ccatgaacaa
     9781 tgttggcgct gcggtcgagg aaatcgatcg cgcggtgttg aagttgggag ccgccggcgt
     9841 gcaaatttat tccaacgtaa atgggcggcc tctggacgat ccggaattcc tgcccatatt
     9901 tgaaaaactt gcagagcgca atgtaccgat gtggctgcac ccgagcaggt cgtccgcctt
     9961 cgccgattac gcgacggaaa agaaatccaa gtttgaactt tggtgggtat tcggatggcc
    10021 gtacgaaacc tccgtggcca tggcacgcat tctatttgcc ggatattttg accgcttccc
    10081 caaccttaaa attatttcac accacctggg cgcaatgatt ccctactttt caggacgcac
    10141 tgggcctggg ctcgatcagt tgggagcgcg caccgaagaa gaggatttgg cgcttgtcgg
    10201 gcgaaaattg caaaagcggc cgcaggaata tttcaaaatg ttttatgcgg atacagcaac
    10261 atttggttcg ctgccggcgc tgcgttgtgg gctggaattc tttggcgccg atcaagttct
    10321 gttcgcgtcc gattctccgt tcgatccgga aaaggggccc ggttacatcc gcgaaacgat
    10381 tcgctgcgtg gacggcgtcg agatgtcaga tgcggatcgg tacaagattt acgagggcaa
    10441 tgcgcggaaa ctcatgcggc tgaagctggc gtagggcatt ttgcctggac gattttgcac
    10501 ggaattatca ccgtcccggc ggagaatcta aacccgcacc tctgaagagg cgggctacaa
    10561 aagctgctgt agcccgggtc ttcagacccg gggtttagag caaacatgat cgcaagctgc
    10621 gcgctaaaca ctgctaacct ctgtcctact ccgtgattcg tttttccggc ggctcgggca
    10681 gccaaaaact gccgataaaa cctacggcgt aagcgaggaa cccgacaatc cccgctgcat
    10741 agccgagttg ccgagtaaca ctcccgccag gcatatacgc aacgatcatt gtggtaatca
    10801 gcgcagcgga cgttccaagc attcgcccgc ctacgttcgc ggcaaagctt tcccctgtgc
    10861 cgcgcagatg cgttggatac agcgtcggca aataattccc ccaaaaactg aattgcgcta
    10921 ccgacacggt accaagcacg aaaattcccc atgaaggcat cgccgtatcc cgcatggcgg
    10981 gaagcagcac gaccaaggga atcaaaatta atcctggaat ctgaaaaatg tgcagcaggc
    11041 gctttcggct gagtataaat atggcgagcc ccgccatcag gattcgccca aacaggcttc
    11101 ccacttcctg aacaccctgc aaggcgctca cgattttctg ctgatccgcg tgagccaaca
    11161 atttcacttc aggcgcaccc ggaataatgc gcgcaaaatg ctgcaacatt ccgaacgatg
    11221 ctgcgtacgc gcaagccatc atgaagcagc ttagaagagc agtcttgcga aattccggat
    11281 ggaataattc caggatgctt ggacgttgca gcgtgccctc ttcccttttc ttctgccaga
    11341 tcggcgactc cggcaaaaac gggcggacga tgagcaatgg aatggccggc agaattcccc
    11401 acagaatcgt atagcgccat gcttcgtgtc caccgtgaat cgctgggagc aaacggccaa
    11461 tggaaaggct caaataactt gcgccgctca tcagtatccc gccagtagac gcaaacacct
    11521 gcgtaaaacc gagaatcgtt tcgcgttgat ctctttccgg aaacagttcg gcaagccaag
    11581 ccgtggcggc cacgaattca actgagacac caacaaaggt aatacaacga taaacgagca
    11641 gttgcatcgc ggacgacgaa taagcagtag ccagcgcgga aaagcagaag agcaaaatgc
    11701 tccagaacaa aacgcggcga cggccaaagc gatcgatcaa atatccgccc agcaagccga
    11761 aaatacctcc ggcaatggct ggaacataga acatcaaacc gatccagtga ttgagttgcg
    11821 cgctgccggc tttggcggat aagaattcgc tcagggcggg ttgcacggtg agcgtaagga
    11881 ccagcaattc atacgtgtca aacgcgaaac cgatgcaagc cacgccgcag atgacccaat
    11941 ccagagggct aagccgccga aatttgctga gtgccgtgga caagatcgcc tgctttccgc
    12001 agcctttacg gtatttggtt cacataaatt tcctgcgtga aaattggatg gccgcgggaa
    12061 cgtaacagac atccaagttt actgtcaatc tgacattcat ttccgtttgg aacgagaaac
    12121 atcgcttcgg ccaaaagagg taaactaaac gtgttcgcgc cgctcggctt gtgattttta
    12181 ctgcatgaca agagcgggct ggagcgcgct tcgcggggca acctctacat gcccaaaggg
    12241 ttcaaaccct caagaagaca ttcaacgatc atgcgaaggc gaacgaaaaa agattcggga
    12301 gagcacgata tggcggatca accgcaccaa attcaaaacc ggcggcagcc agcaaccatt
    12361 ttattgctgg tctcctattt agtttttttt gcgatgcctc agaaagccgc ggcgcagggt
    12421 tcggctggga acaagcctcc cgttgaaaat aaaatgccgc aggacacgat cgactcgagc
    12481 gcgcaggaaa ccaagctggg acctccgact gtgcccgtcg agcaaatcat caagcaattt
    12541 gcggcaaaag aggccgagtt caagatcgag cgcgacaact tcacctacac gcaaaccttt
    12601 gtcatccaga ctctggacga cagcaaccgc gtggatggcg agtaccggat tacgagtgac
    12661 attgtgtttg atgagcgcgg gaaacgggtc gagaaggtga ccaatgctcc cactccgaca
    12721 ctggaacgga ttatgctctc caaggaagat ctaagcgacc tcgaaaatct atatccattt
    12781 gttttaacta gcgatgacat cggtaagtat gacgtcaatt atgtcgacca tgtgcagctc
    12841 gacgaactaa cgacttatgt gtttgatgtg gcgccgaaaa ctctggaaaa gaatcagcgc
    12901 tatttccaag gacggatctg ggtggatgat aaagatttcc agatcgtcaa aacatttgga
    12961 aaacccgtct atgccaagag caggagcacg gaaaaccagg cgtttccccg ctttgaaacc
    13021 ttccgtgaaa acatcgaagg caagtactgg ttccctactt atacgagagc gaatgaaatg
    13081 ctccgcttca agggtggcga tgacgtgcat attaaactga cggtgcgtta tgaaaactat
    13141 aagagattcg gcgtgacggt cagaatcggc gcgcctgtga agcctgcgga accgaaccag
    13201 gaaaaaaaat aagaaggttg cggcattcgt tcatgcgtaa cggaacagcg ctttgaaacc
    13261 gtagtccata tgctgctgaa tgtgacagtg gaaaagcgtc agccccggct gatccgcgac
    13321 catatccacg gaaacgcggc catagccagg caccacgacc gtgtctttta ttatcgccgc
    13381 ggttgccttg ccgttgattt ccaccaattc aaacgagtgc cgatgcatgt gcaacggatg
    13441 cgcatcctcg ctgcgattgc gaaataccag ccgataacga ctgccctgct tcagcacaaa
    13501 ttcctggtca tgaggatatt ctttcccgtt caccgtccag ctgttgaata atcccgcgcc
    13561 actgggaatt ttctgaaaaa tcatgtcgat agtcgaatcg ggcgcgggct gcgttcccgc
    13621 gcgaccaaaa attgtgtaat cccaaacggc tttcacaggc gcagtccatt gcggctgttt
    13681 gtgctgattc gcatattcaa cgatcacgcc catgccgccc atccgcgcat catccgtcgt
    13741 cgtgcccagc acccaaattc ccggctgatt catttccacg atggcatcca cacgctcacc
    13801 cgggccgatg tcaattacat cgacggattg cgacgtggga acggcgttgc catcaagcga
    13861 gacaatttgg aagcgatgtc ccggcagcgc gatgcggcga ttcatgctcg cgctggcatt
    13921 taataaatga atcagcacgc gttgaccggg cttcacgcgg atcggctcgc ctgctcccat
    13981 ggccttgtca ttgatggaaa acatctggta tcccacttcg agaccggaag gatttgtgtc
    14041 tagtgttttg gggcgctcgg gctgcggacc agcattactc gggtcctcgt catcgtcctc
    14101 ttcttcggga ttaaaaaacg gctcccattc cctgagcgct agaaaaactt cctgatcgta
    14161 attacccgga ttattggcgg actctatcat cagaaaaccg aattggcccg tataagcgcc
    14221 gcggtgcagg tccgatccgg ccatgacgtg cgtgtgatac cagcgagtcc ccgcggggcg
    14281 cggcgtaaat tggtagcgtc ggcggccgcg cggcgggacc atgggcgtat tctcttcctc
    14341 tacgccatcc acttcccccg gcaaaaataa tccgtgccaa tggacaagct cagggacatc
    14401 cgtttcattg atcacatcga ccgtgacggg cttcccttcc ttcattcgaa gtacagggcc
    14461 gggagaagtc ccgttgtagc cgatcgtgct gataatacgg gtaggagtga gttctacggt
    14521 gactggcgcg atacgcagcg tgaaatcggc cttaga
//
LOCUS       DASZMB010000144         8377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4814383, whole genome shotgun sequence.
ACCESSION   DASZMB010000144 DASZMB010000000
VERSION     DASZMB010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8377
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4814383"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..2369)
                     /locus_tag="VG272_06600"
     CDS             complement(174..2369)
                     /locus_tag="VG272_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWF13387.1"
                     /translation="MATTAMLTFISFWRAAAIVLNDLASSAYYAGGESESFIGKSAPW
                     FILGVMLFSYCVRAVYVESCSMFVRGGVYRVVKEALGGTLAKFSVSALMFDYVLTGPI
                     SGVSAGQYLAGLLNELSAYFHLGFTLSENTTAACFAVLVTIFFWWENIKGVHESSDKA
                     LKIMQITTVMVAILIVWCVYTIWTRGAQLPPFPTPANIQLDKRSLGWLSGHEIVRMIP
                     MIAVFVGIGHSVLAMSGEESLAQVYREIEAPKLPNLKKAGLIIFIYSLLFTSLVSFFA
                     VMIIPDNVRGKYLENLIGGLSMSLAGPFPLRLAFHVFVVAVGTLILAGAVNTAIVGSN
                     GVLNRVSEDGVLADWFRQPHRKYGTSNRIINIVVIFQIITIIASRGDVTFLANLYAFG
                     VIWSFTMQGIAVLVIRYTHPGDREYRVPLNFHLFGKEIPLGLALITVFLFLIAIVNLF
                     TKPSATMAGLIFSFIMFGVFTVSEKITQKNHGGAHVEMDQFNVTAQEELSRQSVGVRP
                     GSVLVPISNYHALYHLEGVLDRIRPERRDIVVMHVRLLNRTASGSSELEAEQLFGSIE
                     QFLFTKALSLAEKRGKPIRLAVVSANDLWGGILNAAVKLEASTIVLGRSGKVTVAEEA
                     REIGLAWEQLPDPRPKFNLEIFAPGGTREFFLLGPHAPNLTSNEVRLVHRLWLRFSDL
                     VSPEELHHHDVVHFALDEVIKELEEGKEADVAARLREHISKNKAGQTKE"
     gene            complement(2630..3988)
                     /locus_tag="VG272_06605"
     CDS             complement(2630..3988)
                     /locus_tag="VG272_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13388.1"
                     /translation="MQSHQASYAQKRRSTRIDQNVNVIVQGVGALREPYQEQVSTLSI
                     SCHGCTYQSKHEVIQGETVYLDVITSSEGSVACSTKGRVKWAQKVGVKERIFQIAVEL
                     EIAGNIWGIPTPPADWFPPQLPPINEAAAPTPTLKVVARKEQQAIALTTVEASSRAAG
                     VEKREPLAPSIPALAQLMMGLGEQIQTVASEAATATLFKEKSRLIEEFRTQFREEASK
                     GMQSAIATAKELIVRQASKEMSEAHQTIAQNTHAQWAKKIEQELEAAQHHILKQEKET
                     TTRLDGLAAATIERVQRSMEITRTDLVDRFVTRLRDQVEPLLVEAKNSLQKLEASEAA
                     IKKESEIIYSGFGNQLEQSAQSSLSKAQQDLEKNTFAVAARTNETLQKLYQDFEKAAQ
                     SSAESLLASSGNQITEILQGKVNEISQSFSAGLENHTRNYLESVGKSIAEIPNKIPNP
                     SR"
     gene            complement(4128..4556)
                     /locus_tag="VG272_06610"
     CDS             complement(4128..4556)
                     /locus_tag="VG272_06610"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HWF13389.1"
                     /translation="MPIPIDARRKPAKVKVHVSSGAGVDITWADEHVSHYDFAYLREQ
                     CPCAMCNDERQKKAAGQEKDAQLEVENPGAPAAQSLSSPLLPMFKPKIKANAAHAVGN
                     YALRIDFNDGHATGIFSFDYLRTICPCPECALRFRTDPDA"
     gene            complement(4569..5246)
                     /locus_tag="VG272_06615"
     CDS             complement(4569..5246)
                     /locus_tag="VG272_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF13390.1"
                     /translation="MAPVVQVEQLDKRFGDVRALDHISFEVEPGEWIAIMGPSGSGKT
                     TLINILGGLDHPTGGRVVVDGMEIGNLGERELTRYRADKIGFVFQQFHLVPYLTALEN
                     VMLAQYFHSITDEKEAAEALKRVGLGDRLDHVPAALSGGEQQRVAIARALINQPKVIL
                     ADEPTGNLDEANEGVVLKLLHELHEAGHTILVVTHSQAIGNLADRRVELEHGRLARIV
                     SPSLAKD"
     gene            complement(5246..6379)
                     /locus_tag="VG272_06620"
     CDS             complement(5246..6379)
                     /locus_tag="VG272_06620"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF13391.1"
                     /translation="MPSRSMFLRILWKLLGASRGRLAVALIALTSGAAVCSALLNVNL
                     DAERKLTQEFRTLGANVVIAPPAGGSEAALADAAVLDRIATLHLARIAATSPYLYVAA
                     NSGSQPVILAGTWFDQVSKMNFWWKLEGSAVTSRDDHEQCLVGVTAARQLGLSPGSSV
                     QLRSGERNLSLTVAGIVTTGGAEDNQILTNLDSVQTFAGLPGRVSLVQLSVSGTPAEI
                     VEVTRKLAESLPELDVRPVRQIAAAEGTILGKIRGMIFWTITLILILSMLGVLASMAA
                     LAMERRRDVGLMKALGGTVPRIMRLFLAEAGALGAIGGLAGFLLGVVLARWIGERIFG
                     VVISARFEVLPITILLTMGVALAGSFPLRLLGRVRPAEILRGE"
     gene            complement(6383..7690)
                     /locus_tag="VG272_06625"
     CDS             complement(6383..7690)
                     /locus_tag="VG272_06625"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF13392.1"
                     /translation="MFFRIVSDSFTRKPRRKILTAAALALGMAVATATLEVALDVGDR
                     LAREFRSLGANLLVTPASDTLPLEIGGVDYRPVDAGAYLPAGDLGKLKTIFWRNNVMG
                     FAPFLDVPVVIASPDLKNDSQKVSQKDSHEELIGTWYSHSVSVPDGSTFTTGISVTDP
                     WWHVDGRWFSDGAEEGVAGSSLAQRLNLRAGSKIKIDAGSRTHALTITGIVSTGGRED
                     NSILAPLEVAQDLAGRSGEYRRLLVSALTKPADSFSERDPAGMTPVEYDRWYCSPYIS
                     SISHQIQQQLPGVDVRAIRQVAEGEGRILTRVSGLMWMVTLAALFAAALAVGATSATT
                     VLERRSEIGLMKALGASSRAVSSFFIAEQLFLALVGGILGYALGILLARALGIGIFGV
                     APVLRWILLPIVLALAAVVALLGSLLPLGRASRVDPAPVLRGE"
     gene            complement(7880..>8377)
                     /locus_tag="VG272_06630"
     CDS             complement(7880..>8377)
                     /locus_tag="VG272_06630"
                     /inference="COORDINATES: protein motif:HMM:NF021587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2318 domain-containing protein"
                     /protein_id="HWF13393.1"
                     /translation="AADRRRLEWERRKQRRWALASAFTCTAVILILAAEFAYTRVTAT
                     TEARSIQAENGAVRIPMSEISDANLHIYSVDASGTSVRFMVIRKPNGSWGTALDACLI
                     CGTAGYRQEGSNVICRNCASAIYIPTIGEAGGCNPVGVPSHAENGALVIDVSTLAEAR
                     SRVSK"
BASE COUNT         1932 a         2474 c         2196 g         1775 t
ORIGIN      
        1 atccccacgt tccagcgccc gcaacacgcg ttcccgtaaa tcctgcgaat acgcttgcat
       61 ccgctcctcc tacgaaaggc aggagcatta tcgcacagat gttcatactt aaattagaaa
      121 tgctctagaa aatcagaagt gttaggaaag aaaaaggctg gaaaactgca acactactct
      181 ttcgtctgcc cggctttatt cttgctgata tgctcccgca ggcgcgctgc gacgtccgct
      241 tccttgcctt cttccaattc cttaatcact tcatccagtg caaagtggac gacgtcgtgg
      301 tgatgaagtt cttccgggga tactagatcg ctgaagcgca gccagagtct gtgcacgaga
      361 cgcacttcgt tggaagtaag attcggcgcg tgaggaccga gcaggaagaa ttcgcgcgtt
      421 ccccccggcg cgaagatttc gagattaaat ttcgggcgcg gatccgggag ttgttcccac
      481 gcgagcccga tttcccgtgc ctcttccgca actgtaacct tccccgatcg tccgagaaca
      541 attgtagagg cctcaagctt gacagccgca ttgagtatgc cgccccaaag atcgtttgcg
      601 gaaaccacag ccagacgaat cggtttcccg cgcttttccg ccagcgacag cgcctttgta
      661 aacaggaact gctcgatgct gccgaaaagt tgctcggctt caagttcgct cgagcctgag
      721 gctgtccggt tgagcaggcg cacatgcatc acaacgatgt cgcgccgctc cgggcggatg
      781 cgatccaaaa ctccctccaa atggtagaga gcatgataat tgctgatcgg aaccaaaacg
      841 cttcccggcc ggacgccgac cgattgcctc gacagttctt cttgagcggt cacgttgaat
      901 tgatccattt caacgtgtgc tccgccgtgg tttttttgcg tgattttttc agatactgta
      961 aacacaccaa acatgatgaa agaaaaaatc agtccagcca tagtagcgct tggtttcgta
     1021 aaaaggttca caatggcgat caaaaataaa aagaccgtga tcaaggccag gcccagcgga
     1081 atttccttgc cgaacaaatg aaaattcaac ggcacgcggt attcgcgatc gccgggatgc
     1141 gtgtaccgta ttacgagcac cgcaatgccc tgcatcgtga aactccagat cacgccgaac
     1201 gcgtacaggt tggcgagaaa cgtcacgtcg ccacgactgg caatgatcgt tatgatctga
     1261 aagattacaa cgatgttgat gatgcggttg gacgtcccat attttcggtg cggttgccgg
     1321 aaccagtccg ccagcacgcc gtcctcggaa acacggttga gaacgccatt cgatcccacg
     1381 attgcggtat tcacggcccc ggcaaggatc agcgtcccga cggccacgac aaaaacatga
     1441 aacgcgaggc gcagcgggaa cggacccgcc aggctcatgg acaatccgcc gatcagattc
     1501 tccaggtact tgccccgcac gttgtccgga ataatcatca cggcaaaaaa cgatacgagt
     1561 gaagtaaaca gcaggctata aataaaaatg atcaagccgg ccttttttag gttcggcagc
     1621 ttgggcgctt cgatctcacg atacacctgc gcgagtgact cttccccgct catcgccaga
     1681 accgagtgac cgatgcccac aaacacggca atcatcggaa tcatgcggac aatttcatgt
     1741 ccggaaagcc accccaggga ccgcttatcc agctgaatgt tggcaggcgt gggaaacgga
     1801 ggaagttgcg cgccgcgagt ccagatcgtg tacacgcacc atacgatcag tatggccacc
     1861 attaccgtgg tgatctgcat gattttcagc gctttgtcgc tggattcgtg aacgcccttg
     1921 atgttttccc accagaaaaa aatcgtcacg agaacagcga aacatgccgc cgtcgtattc
     1981 tccgaaagcg tgaagcccag atgaaagtaa gcagacaact cattgagcaa cccggccaga
     2041 tattgccccg cggatacacc gctgatcgga ccagtcaaaa catagtcaaa catcagcgcg
     2101 gagacggaaa atttcgcgag agtgccgcca agcgcttcct tcaccacgcg gtaaacgccg
     2161 ccgcgcacga acatgctgca gctttcaacg tataccgccc gaacacagta ggagaacaac
     2221 atcacaccga ggatgaacca tggcgcggac tttccgatga agctttcgga ctctccaccc
     2281 gcgtaatagg cggacgaggc aaggtcattc aacacgatgg cagcggcacg ccaaaaggaa
     2341 atgaaagtga gcatggccgt ggtggccaca atgacgcgag gtacggttat aagcggtgaa
     2401 ggttcagtat tttggccggc aggcgtggat ggcataagtc gtaaggattc tataaaggat
     2461 tgattatggc atctttatat gagggtgtcc acggcaagcg gccaaaacac agctgtaatc
     2521 gaaggagttt tttgaatgga gttcatccaa ttttgaaatg cggatgccaa gctgcagcct
     2581 tcacaagcta cgcgagtcga aaaaaatcga gcgataacct gtgcgcgaat tagcgcgaag
     2641 gattcggtat cttgtttgga atctcggcaa ttgatttgcc gactgactcc agataattgc
     2701 gtgtatggtt ttcgagcccc gcggaaaacg attgtgaaat ctcgttcact ttcccctgca
     2761 aaatttcggt gatctgattc ccactggaag caaggagcga ctccgcgctg ctctgcgccg
     2821 ctttctcgaa gtcctgatac agcttctgca gcgtttcgtt tgtcctggcg gccactgcaa
     2881 acgtgttttt ttccaaatcc tgctgtgcct tagacaggct cgactgggcg ctttgttcca
     2941 gctgattgcc gaaaccggaa taaattattt ccgattcctt tttgattgca gcctcggaag
     3001 cttccagttt ttgcagggaa ttcttggctt ccaccagtaa cggttccacc tgatcccgca
     3061 ggcgcgtcac aaatcgatcc actagatcgg tgcgcgtaat ttccatgctt cgttgcacgc
     3121 gctcaatcgt cgcggccgcc aaaccgtcca gacgcgtggt tgtctctttt tcctgcttca
     3181 agatgtgatg ttgggcagct tccagttcct gctcaatctt ttttgcccat tgggcatgcg
     3241 tattttgcgc aatggtttga tgcgcctcgc tcatttcctt cgaggcctgc cggacaatca
     3301 gttccttggc cgtcgcgatc gcggattgca tgcccttgga agcttcttcc ctgaactggg
     3361 tgcgaaactc ttcgatcagg cggctctttt ccttgaacag cgtggcggtg gccgcttcgg
     3421 atgccaccgt ctgaatctgc tcgccgaggc ccatcatcaa ttgcgcaagc gccggaatgg
     3481 aaggcgcaag gggctctctt ttttccacgc cagcagcccg actactcgct tccaccgttg
     3541 ttagggcaat tgcttgctgc tcttttcgcg ctactacctt taacgtaggt gtgggcgcag
     3601 ccgcttcgtt gattggcgga agctgaggcg ggaaccaatc cgcagggggc gtcgggatgc
     3661 cccagatgtt ccctgcgatt tccagttcca ccgcaatctg gaagatgcgt tccttaactc
     3721 cgactttttg tgcccatttc acgcgcccct tggtcgagca cgccaccgag ccttccgaag
     3781 aggttatgac atccaaataa accgtttcac cctggatgac ctcatgcttg gactggtaag
     3841 tgcagccgtg gcaactaatg gacagggtcg aaacctgctc ctgataaggt tcgcgaagcg
     3901 ctcctacacc ctgcacgatg acgtttacgt tctgatcgat tcgagtgctg cggcgctttt
     3961 gcgcgtatga ggcctggtga gattgcacga aatcctccgt agggctggaa taaaaatgat
     4021 acgccggaat ccacgcggag gtctgcgctg ctggagagcc ctgttgctga ggagttactg
     4081 tccctgattg ctgtctctga ttgaacattg ccgccaagga atggacgtta tgcgtcgggg
     4141 tcagtccgga atcggagcgc gcattcgggg cacgggcaaa tcgttctgag gtaatcgaag
     4201 ctaaaaatgc ctgttgcgtg gccgtcgttg aaatcaatcc gcagggcgta attccccacc
     4261 gcgtgcgccg catttgcctt gattttaggt ttaaacatgg gcagcagggg cgaacttagt
     4321 gactgggctg ccggagcgcc cggattttca acttctaatt gcgcgtcttt ttcctggccg
     4381 gcggcttttt tctgccgctc gtcattgcac atcgcgcagg gacactgctc gcgcaggtac
     4441 gcaaaatcgt agtggcttac gtgttcatcg gcccaggtga tgtccacacc tgctccggat
     4501 gaaacgtgga cttttacctt ggccggtttt cttcgagcat cgataggaat aggcatgagt
     4561 atcgttcgtt agtccttcgc cagcgaggga gaaacaatcc gggctagtcg gccgtgttcg
     4621 agttccaccc ggcggtccgc aagattcccg atggcctgcg aatgggtgac gaccagaatc
     4681 gtgtgcccgg cctcgtgcaa ctcatgcagc agtttcagca cgacgccctc attcgcttcg
     4741 tccagattgc ccgtcggctc gtcagccaga ataactttcg gctggttgat cagcgctcgc
     4801 gcgatggcta cgcgctgttg ctccccgccc gaaagcgctg cgggaacatg atccagtcga
     4861 tcgccaagcc cgacccgctt aagcgcctct gcagcctctt tttcatccgt gatgctgtga
     4921 aaatattgcg ccagcatcac gttttcgagc gccgtgagat atggcacgag atgaaattgc
     4981 tgaaacacaa aaccaatttt atcggcgcga taacgcgtca actcgcgttc gcccagatta
     5041 ccgatttcca taccgtcgac aaccacgcgg ccaccggtcg gatggtccag cccgccaaga
     5101 atattaatga gcgtcgtctt ccccgaaccc gaaggcccca taatagcgat ccactcgccg
     5161 ggttcgacct caaagctgat gtggtcgagc gcgcgcacgt cgccgaagcg cttgtcgagc
     5221 tgctcgactt gtacgaccgg tgccatcact ctccccgcaa aatttccgct gggcgcactc
     5281 gccccaggag acgcagcgga aacgatccag ctagcgccac gcccatagtc agtaaaattg
     5341 taatgggcag aacttcgaac cgtgccgaaa tcacgacgcc aaaaatccgc tcgccaatcc
     5401 atcgcgcaag gaccacgccg agcaaaaaac cagccagccc gccaatcgcg ccaagcgctc
     5461 ctgcttcggc aagaaacaat cgcatgatac gcggcactgt gccacccaag gctttcatca
     5521 ggcccacatc gcgccgccgc tccattgcca gcgcggccat cgacgccagc actccgagca
     5581 tggaaagaat cagaatcagc gtaatggtcc agaaaatcat accgcgaatc ttacccagaa
     5641 tcgtcccttc cgcagcggca atctggcgca cgggccggac atccagttcc ggcaagctct
     5701 ccgcgagctt gcgcgtgact tccacaattt ccgccggcgt gcccgaaacg ctcaattgca
     5761 cgaggctcac gcggcccggc aaacccgcaa aagtctgcac ggaatccaga ttggtgagga
     5821 tttgattgtc ttcggcgcca cccgtcgtca caattcccgc gaccgttagt gaaagattgc
     5881 gttcgcccga gcgcaactga acggagcttc ccggggagag ccccaactgg cgcgcggcgg
     5941 taactcctac cagacactgc tcgtgatcgt cacgcgaagt gaccgcgctt ccctccagct
     6001 tccaccagaa gttcatcttg gaaacttggt cgaaccaagt gcccgctaaa atgacgggtt
     6061 gcgatcctga attcgccgcc acatacaggt agggcgaggt ggccgcgatt cgtgcgagat
     6121 gcagtgtggc gatgcgatcc aggacagcag catcggcaag agcggcttca ctaccgcccg
     6181 ccggaggagc gatcacgacg tttgcgccca gtgtgcgaaa ttcctgcgtc agctttcgtt
     6241 ccgcatccaa atttacattg agcaaagccg aacaaacagc agcgccactc gtgagcgcga
     6301 tgagagccac ggcaaggcga ccgcggctgg ctccgagcaa cttccacaaa attcgcagga
     6361 acatcgaacg gctgggcact actcactctc cccgcaaaac aggagcggga tcgacgcgcg
     6421 aggcgcgccc aagcggcagg aggctcccaa gcagagcgac gaccgctgcg agggccaaaa
     6481 caatgggcag caaaatccag cgaagcaccg gagcaacgcc aaaaattcca atgccaagcg
     6541 cgcgcgccag caaaattcct agcgcgtatc ccaggatacc tccgaccagc gcaagaaata
     6601 attgctccgc gataaaaaac gaactgaccg cgcggctact cgcgccaagc gccttcatca
     6661 acccaatttc cgaacgccgc tcaagaacag ttgtcgcgga ggttgcccca accgcaagcg
     6721 ccgccgcgaa taatgcggca agcgtcacca tccacatcag cccgctgact cgcgtaagaa
     6781 ttcgcccttc gccctccgca acttgccgga tcgctcgcac atccacgccg ggaagctgtt
     6841 gttgaatctg atgcgagatg gacgaaatat acgggctgca ataccagcgg tcgtactcga
     6901 cgggcgtcat gccggccgga tcgcgctctg aaaatgagtc cgcgggtttc gtcaaagcgc
     6961 tcacgagcag ccgcctgtac tcgcccgagc ggcctgccaa atcctgcgcg acttccagag
     7021 gagcgagaat cgagttatct tcgcggcctc cggtcgaaac aattcccgtg atcgtaagcg
     7081 cgtgtgtgcg gctgcccgca tcgatcttga ttttgcttcc cgcgcgcaaa tttaaacgct
     7141 gcgcgagcga tgaccccgcg acgccttcct ccgcgccatc ggaaaaccag cggccatcca
     7201 catgccacca cggatcggtc acagagattc cagtcgtgaa tgtgctgccg tcaggaacag
     7261 aaacagagtg gctataccac gtcccaatta attcttcgtg cgaatctttc tgtgaaactt
     7321 tttgcgaatc attctttaaa tctggcgacg cgatcacgac aggaacatcc agaaatgggg
     7381 cgaatcccat cacgttgttg cgccaaaaaa ttgttttcag tttgcctaaa tcaccggcag
     7441 gcaaataggc gcccgcatcg acaggcctgt aatcgactcc accaatttcc agaggcagtg
     7501 tgtccgaggc cggagtgacc agcagattcg cgcccagcga acggaattcc cgcgcgaggc
     7561 ggtctccgac gtccaaggcg acttccagtg tggcagtcgc aacggccata cccagcgcaa
     7621 gagctgccgc cgttagaatt tttcggcgcg gctttcgcgt gaatgagtcg ctgacaattc
     7681 gaaaaaacat ggtcggctgt gccttgcgag aatcggttgc cctgatctac agaaaacccc
     7741 cgcctctgac ccttcgcgtt ctctcagggc aagagaggcg ggctacagct gcttttgtag
     7801 cccgggtctt cagacccggg gcttttctgc cgcgtgggca acatttctaa tgcaaagaca
     7861 atttctcatc tataaaattc tacttcgaaa cgcggctcct ggcttcggcc agcgtcgaaa
     7921 cgtcaatcac cagcgcgccg ttctccgcgt gcgacggaac ccccacagga ttgcatccac
     7981 ccgcttctcc aatggtagga atgtaaatgg cagacgcgca attgcggcaa atcacattcg
     8041 aaccttcctg acgatatcca gccgtgccgc aaatcaggca ggcatccagc gccgttcccc
     8101 agcttccatt aggctttcga atgaccataa aacgcacgct ggtaccgctc gcgtccacgc
     8161 tgtaaatgtg aagattcgca tcgctgattt ccgacatggg aattcgaacg gccccatttt
     8221 ccgcctggat cgaacgagct tcggttgtag cggtcacgcg cgtatacgcg aattcagcgg
     8281 ctaaaatcag gataactgcc gtgcaggtaa atgccgaggc cagcgcccag cgacgctgct
     8341 tacgccgctc ccattcaagg cgccgtcgat ccgccgc
//
LOCUS       DASZMB010000145         4011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3446470, whole genome shotgun sequence.
ACCESSION   DASZMB010000145 DASZMB010000000
VERSION     DASZMB010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3446470"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..1333)
                     /locus_tag="VG272_06635"
     CDS             complement(191..1333)
                     /locus_tag="VG272_06635"
                     /inference="COORDINATES: protein motif:HMM:NF027716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HWF13394.1"
                     /translation="MISLDVFRGAAIAAMILVNDPGSWSHIYPPLEHAEWNGWTPTDL
                     IFPFFLFIVGVSLTLSFAARIARGISRRALVLHVVRRSAVIFLIGLFLNGFPDFDFSS
                     IRIMGVLQRIALCYLVAGLLYLFTFQTEPRADGSTYVQANIRVMAIVALSLVIGYWAL
                     MTFIPVPGYGAGHLGKDDNLGAYVDRSLMGGHLWSESKTWDPEGILSTLPAIASLLVG
                     ILAGEWLRSDRDAARKTLGLAAAGLALLIVGRLMHPFFPINKNLWTSSFVLFTGGFAM
                     LALAACYWIIDVRGFRAWAKPFLVFGMNAILAYALAALVSEVSTDFEFTNSRGRETTG
                     HGWIYQRFFVAHFNPLNASLGFAIFFVLIIFVLVWPFYRWKIFLRI"
     gene            complement(1768..2301)
                     /locus_tag="VG272_06640"
     CDS             complement(1768..2301)
                     /locus_tag="VG272_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13395.1"
                     /translation="MPATISASSGLTSVDDSVTADEVTAAETTILAGEPVAALPEAPM
                     PVEPVQMNAPIAFLNPAKPMKVSVEQLIAENRRKQMAWKGLAFAASGAATFDAWSTQH
                     AITKEGAVELNPMLKPFAGNASLYAAIQVGPALMDFAGKKMMYSRYSWVRHMWWVPQG
                     ASFVSSIFCGAHNLSFH"
     gene            complement(2603..2755)
                     /locus_tag="VG272_06645"
     CDS             complement(2603..2755)
                     /locus_tag="VG272_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13396.1"
                     /translation="MHRTKRGRILEFWVMLFLPYRPYRCGKCRQRFYGPKHLPKPDVL
                     DDLDSQ"
     gene            complement(2904..3365)
                     /locus_tag="VG272_06650"
     CDS             complement(2904..3365)
                     /locus_tag="VG272_06650"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF13397.1"
                     /translation="MVQVETFQEFRAGLSLLRGGDANKALPHLKTAFEGEPANPFYIS
                     YMGVAIAATEQKWAEAEELCRSAIRMSRRQAQLYLNLAEVYVGADRKQDAADTLVRGL
                     RYAPHDRRLKMGLDRLAIRRPPVLRFLPRTHSVNRGLGRLRHQALQVLATF"
     gene            complement(3717..>4011)
                     /locus_tag="VG272_06655"
     CDS             complement(3717..>4011)
                     /locus_tag="VG272_06655"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HWF13398.1"
                     /translation="NIVAHVRMDGAWIGSIDISMKKAYTSRAFDITTKDLAAHSQSGG
                     QFFGIHASNDGKIMIFAGGIPLKKDGKVVGAVGVSGGSGDQDHAVAEAGAKAY"
BASE COUNT         1034 a         1058 c         1075 g          844 t
ORIGIN      
        1 gttacggcaa ggttaaaaga aaaaggccgc ctttgagcgt agtgtaccaa attgacctta
       61 accggcaggg gcaaaaagcc gctcgcccga aatgcggtcg gccgctacaa aaacaaaatc
      121 gttcaagcag tggggtttcg tagagagcac agtgcgccgc aaattgcgat gttgctcgat
      181 ctcaacttac tcaaattcgc agaaaaattt tccagcggta aaatggccaa acgagtacaa
      241 aaattattaa aacaaagaat atcgcgaagc ccaacgaagc gttcagcgga ttgaaatgcg
      301 caacgaaaaa tcgttggtaa atccacccat gtccagtggt ttcacgtcca cgggaattgg
      361 tgaattcaaa atccgtgctt acttcggaga cgagggcagc gagggcatag gccagaattg
      421 cgttcatgcc aaacacaagg aatggctttg cccatgcgcg gaaaccgcgc acatcgataa
      481 tccaatagca tgcggccagc gcgagcatag cgaacccgcc ggtgaaaagc acaaacgagc
      541 tcgtccacaa atttttgttg attgggaaaa atggatgcat gagccgcccc acaatcagta
      601 gcgccaaacc tgcggcagcc aggcccagcg ttttgcgcgc agcgtcacga tccgatcgca
      661 gccactcgcc cgcaagtatt cccaccagca aagaagcgat tgccgggagc gtggaaagaa
      721 ttccctcggg atcccacgtt ttggattccg accacaaatg tccgcccatc agcgagcgat
      781 cgacgtacgc gccgagatta tcgtccttgc ccaggtgccc cgcgccatag cccggcactg
      841 gaatgaacgt catcagcgcc caatatccaa taacgagact taatgcaaca atcgccatga
      901 cgcgaatatt tgcctgcaca tatgtggacc cgtccgcacg tggttccgtt tgaaatgtaa
      961 ataaatagag caatcccgcg acgagatagc agagcgcgat gcgctgcaag actcccatga
     1021 ttcggatcga gctgaaatcg aagtcaggaa atccattcaa aaataatcca ataagaaaaa
     1081 tcaccgcgct acggcgcact acgtgaagca caagagcacg gcgagaaatt ccacgcgcga
     1141 tgcgcgccgc aaatgacaag gtcagcgaaa cgccgacaat aaaaagaaag aaaggaaaga
     1201 tcaggtcggt gggagtccag ccgttccact cggcgtgttc cagcggagga taaatgtggc
     1261 tccaggagcc ggggtcgttc accagaatca tcgcggcaat cgcggcgccg cgaaaaacat
     1321 ccagcgaaat catgcgctca gggcggcgtg aattcggaat ctccgtttga ccggacgcga
     1381 cagctgcact gttctccgcc gagcgaatgg tgaactcctc caatggccgg atagtatagc
     1441 agcagaggaa atgtcacaat ggctcgcggc gatttgaaaa gaaaagttga tggagaaagg
     1501 aaactcaggg gctgagcttc cttaattctc tgtcagtgcg acaggggcta aagcccttgt
     1561 ttttcaagtc tctgaatgtc gcggcttgaa gccgcgaccc acaagacgat ttatgaaatg
     1621 acatggctct gtccatatta ccggttccgc tgccgatctg ctgtagcccg cctcttcaga
     1681 ggcggggttt ttggcgctcc agcatctaaa cccccgcctc tgatccctcg tttttactcg
     1741 ggataagcgg ggcgagctac aattccccta gtgaaaactc aggttgtgcg cgccgcagaa
     1801 aatcgagctg acgaagctgg cgccttgcgg aacccaccac atatggcgaa cccaggaata
     1861 gcggctatac atcatttttt tgcccgcaaa atccataagc gctggaccca cttgaatggc
     1921 cgcatacaga gaagcattgc ccgcgaacgg cttcagcatc ggatttaatt ccactgcacc
     1981 ctccttcgta atcgcatgct gcgtggacca tgcgtcaaag gttgccgcgc cgctggcagc
     2041 aaaagccaag cctttccaag ccatttgttt gcggcgattt tcggcaatta attgctcgac
     2101 agaaactttc atgggctttg ccggattcag gaacgcgatt ggtgcattca tttgtaccgg
     2161 ctccactggc ataggagcct caggcagggc ggcgacaggc tcgcctgcga gtatcgtcgt
     2221 ttcagcggcg gtcacttcat cagcggtcac tgaatcatca acgctcgtca agccggatga
     2281 cgcggaaatg gttgcaggca tatgcaatgc tgccacttca gtggacgtag aaatcttggg
     2341 agcaatctgc ggatgaagca aaagaaaggc aaggggtaga aacattgtaa tgatcctcag
     2401 tacgctgcaa aaactccggt gattcatttc ttgcagcgtg aggctagcat agaagcgtgg
     2461 ggtggtcccc gccatggaac ggcggtcctg aagaagtgta acgatggttt ttgccaagcc
     2521 ggaactatag ttctaggact attgggggat ggagaaaatg ccactcggcc cggtggggtc
     2581 gaagtcgcgt catttcaatt tgttactggc tgtccagatc gtcgagaaca tccggcttgg
     2641 gcaggtgctt cgggccataa aaacgctgcc ggcacttgcc gcaacgatag gggcggtagg
     2701 gcaagaaaag catgacccaa aattccagga tgcgcccgcg tttggttcga tgcacgtggt
     2761 tactaccaca atgagggcaa tttctatctt ccgtgtaaac gaagaggcca agtttgcgct
     2821 tcattaatgg agaataataa cgccgggcga ctggctaagc aatcgcccgg gtcatttatt
     2881 ctatggtaaa gtaatctaat tgttcagaag gtggccagaa cctgcaaggc ttgatgtcga
     2941 agtcttccca gaccgcgatt caccgaatgc gtgcgcggta gaaaacggag tacgggtggt
     3001 cgccgaatgg ctaaccggtc aagtcccatt ttcaatcgtc gatcgtgagg agcgtagcga
     3061 agtccgcgca ctagggtatc cgccgcatcc tgcttgcggt cagcgcctac atagacttcg
     3121 gcgagattta aatacagctg agcctgccgg cggctcatgc gaatggccga gcggcaaagt
     3181 tcttccgctt ccgcccactt ttgttcggtg gccgcaatgg ctacacccat ataagagatg
     3241 tagaagggat tggcgggttc tccctcgaat gccgttttga gatgggggag ggctttgttc
     3301 gcatcgccgc cgcgcaaaag cgacaggccg gctctaaact cctgaaaagt ctcaacctgg
     3361 accatataga tttcctccac tgggctactg cgaagtgccg ctgttcgcca caccgcgctg
     3421 cgggtaattc cggaaacgtt gcaaggaaga ttaaaagcgg ggagcctatc gggcaactga
     3481 tcgcatggac agaagaagta cagtccaaac gctttaccaa cacttgagaa aacggtcagc
     3541 acatgttcac agaacaagca acggacctta gatgccgatg ttgcaaccat tttgagccag
     3601 taagctggtc caactctggg gtttaaatcg aaagccccgc cctcaactga tagaggacgg
     3661 ggcatgcggt tactgactgc catatttaat tccactgcaa gtcaggccgc ttaaaattaa
     3721 tacgcttttg cgccggcttc agccaccgcg tggtcctgat cgccggagcc gccgcttaca
     3781 cctacggctc ctactacttt gccgtctttc ttcagaggaa taccgcccgc gaagatcatg
     3841 atttttccgt cgttcgaagc gtgaattccg aagaactgtc cgcccgattg cgaatgcgcg
     3901 gcgaggtcct tggttgtaat gtcaaatgcg cgcgacgtgt aggccttctt catcgagatg
     3961 tcgatgcttc cgatccatgc gccatccatg cgcacgtgag caacgatatt t
//
LOCUS       DASZMB010000146        11045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1527555, whole genome shotgun sequence.
ACCESSION   DASZMB010000146 DASZMB010000000
VERSION     DASZMB010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 11045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11045
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527555"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /gene="arsB"
                     /locus_tag="VG272_06660"
     CDS             <1..1028
                     /gene="arsB"
                     /locus_tag="VG272_06660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HWF13399.1"
                     /translation="TSLPFLDRFLTLWIFLAMAIGVALGHFIPQTATLVQDFQIGTTN
                     IPIAIGLIIMMYLPLAKVRYEKLGEVFRNRRVLGLSLLQNWVIGPILMFFLAVTFLCG
                     EPAYMRGLILIGLARCIAMVIVWNELAAGDTDYAAGLVAFNSVFQVLFYSVYAWAFIT
                     VLPKWFGLEGSVVHVGIGQIAQSVFFYLGVPFLAGIITRLLFLRAKGEEWYRTQFISR
                     ISPVTLVALLFTILVMFSLKGDLIVRLPLDVLRVALPLLIYFALMFLVSFWMGRKLGA
                     DYSKTATLSFTAASNNFELAIAVAVAVFGLNSGEAFVGVIGPLVEVPALLGLVNVSLW
                     FRRRYFA"
     gene            1100..2044
                     /locus_tag="VG272_06665"
     CDS             1100..2044
                     /locus_tag="VG272_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF13400.1"
                     /translation="MPESASQSALRVRQLRKAYKDVLAVDGLNLEVHAGECFGLLGPN
                     GAGKTTTVEICEGLTDPDAGDVEVLGMRWSSDAAQLRQRLGIQLQDTQLSEKLTVHET
                     VRLFRSFFQQGADATDVIARVQLEEKQNSRVGDLSGGQKQRLALACTLVGDPDFLFLD
                     EPTTGLDPQARRQLWELIEEFKESGRTILLTTHYMDEAERLCNRVAIVDHGKEIALGS
                     PRELIASTCAEHVVEFTAGSASHALDVSALRRIEGVREVRTQKDAVLLQVSELHTAVP
                     ALLAELTRQNVPLTEFRTHSATLEDVFVALTGRHLRDE"
     gene            2037..3128
                     /locus_tag="VG272_06670"
     CDS             2037..3128
                     /locus_tag="VG272_06670"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF13401.1"
                     /translation="MSENTISEVRAATEKKSPRANSFADHPLVQLTLVRFLEFIREPE
                     ALFWVFAFPVLLAAGLGIAFRNRPADVLKIAVVSPGLAQSLQREKLLEIQQLDESVAQ
                     EALRTGKVALLALPGSAGTVEYRYDDTNPEGRAARLLADRAIQRAAGRADPVATSDQL
                     MREPGSRYIDFLIPGLLGMNLMGSAMWSIGFAIVDARRKKLLKRLVASPMPRPYYLIS
                     FLLSRILMLVLEVGVLVGFGALFFHVPVRGSLLTLATLCVLSSLSFCALGLLLASRVK
                     TIEAASGLMNVVMMPMWIASGVFFSSQKFPGLMQPFIKALPLTAVIDALRSNMLQGAN
                     LSQMMPQVGTILFWLVICFALALKIFRWR"
     gene            complement(3340..4677)
                     /locus_tag="VG272_06675"
     CDS             complement(3340..4677)
                     /locus_tag="VG272_06675"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HWF13402.1"
                     /translation="MSTVQKQAEAFDARHTIKRARKAAHVLATLSGERRNEALIAAAN
                     AIEARTAEIQAANDKDCAAAQKAVELGELSRAMFARLRTTERGIADMSARVRDVARLP
                     DPLHRRLSTMELDDDLVLHKETCPLGVVGVVFESRPDVVPQISSLALKSGNAVCLKGG
                     AEAAETNAVLVSIWRDALSAFSDIPVDSVVLIHTRQDVAEILKMHEDIDLIVPRGSNE
                     FVTYVMQNSLIPVLGHGEGICHVYVHSDADVQKAIDVSYDSKVQYPAVCNAMETLLVH
                     QEIAPLFLPLMIEEFQNAGVEVRGCARTLALTKARGIIPATEEDWKKEYSDLIVSVKV
                     VDGVNQAIDHINKYGSRHTESIVTESHDVAARFMEFVDAANVFQNASTRFSDGFRYGL
                     GAEVGISTGKLHARGPVGLDGLTIYKYKLFGKGQTVASYNKGQRTFKHRLIPG"
     gene            complement(4674..5864)
                     /gene="proB"
                     /locus_tag="VG272_06680"
     CDS             complement(4674..5864)
                     /gene="proB"
                     /locus_tag="VG272_06680"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HWF13403.1"
                     /translation="MENQTSETEQQKRLSASRRAVIKLGTGIVTDSDGHFNIAQLEPV
                     ARGIAQLKKDGRQIVLVSSGAVGLGRGRLGLHRDRLNDMVMRQACAAVGQSLLMHEYE
                     KLFQQFGVHIAQVLLTEGDFVDRTRYSNLRETMEALLKLGVLPIVNENDTVSTAELDY
                     LNIRAGERIFSDNDRLAALVMSHVEADALVLLTDVDGLMQLGPSGAIDADAKIIPLVE
                     EINSELKALALGPSEGGRGGMLTKLEAAEIAMQAGGVAVITNGTKPDALMKVFNGEPA
                     GTVFVSKTRMAGKRRWIAYAAGVRGRVIVNAGAREALTGGKASLLASGVTGVEGEFDA
                     AEVVSIVDGEGVEFARGITDYSRGDAQGLIGNGSRVTDVPGQTSKRASVLVKRDNIVL
                     LDRK"
     gene            complement(5941..6540)
                     /gene="recR"
                     /locus_tag="VG272_06685"
     CDS             complement(5941..6540)
                     /gene="recR"
                     /locus_tag="VG272_06685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HWF13404.1"
                     /translation="MPDFAEPVERLIDQLKHLPGIGAKTAQRLAFHILRTDSENALAL
                     ADAIRDAKMNMRVCTVCNNITDVDPCLYCTSSTRNRKTICVVEEPHNIMAIEKTRQYN
                     GMYHVLGGSLAPLRGVGPDQLHLKNLIERLKGGVVEEIIIATNPNAEGEATAMYLSKL
                     LKPLGVRVTRIGVGIPVGADLEYADEVTMMKAMEGRRDL"
     gene            complement(6631..6957)
                     /locus_tag="VG272_06690"
     CDS             complement(6631..6957)
                     /locus_tag="VG272_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HWF13405.1"
                     /translation="MEVKALQQILTQFRQMQEGLQKQVETVHVEASAGGGMVTVKMNG
                     QKQLTDVHIDSEIFASKDQELLQDLVRSAVNEASRRIDEELTSQMKNLTGSIPGMAGL
                     KIPGLF"
     gene            complement(6961..8802)
                     /gene="dnaX"
                     /locus_tag="VG272_06695"
     CDS             complement(6961..8802)
                     /gene="dnaX"
                     /locus_tag="VG272_06695"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HWF13406.1"
                     /translation="MSYQVIARKWRPQTFADVVGQQHVTRTLSNAIQSGRVAHAYIFS
                     GARGVGKTTTARILAKALNCAKGPAAEPCNECGSCREISQGNSLDVIEIDAASNRGID
                     QIRELREMVRYAPAGGRYKVVILDEAHALTGEASNALLKTLEEPPDKVIFVMATTEPE
                     NLADTIRSRSQHFHFRALSFAEISDALNDICAKEGLTAESGAIAVMARAAEGSVRDGL
                     SLLEQARAYCGTTITDAQVRELLGVVPGEILDELTHAIEERSAERVLSLVHRLLAEGQ
                     NLQHFCREAIGHFRNLLVARVCGADSELIAAPVDERPRLKEAAEFFSEEDLTRFFQIL
                     LHTDDDVRRKPDPRLHLEMGLLRMVNAARLAPLEELLADLDNGTQANSVPTRSSSALP
                     QTPAPARSSSAPASSVSATWQSPAISQGAATATAPAKTQAARVETTVPFTAKREEINF
                     SPKPIDATPAPPPAVTTDTVARPDAFGSGPLEEKINSIKGAIVAQEKFLGELVVHASH
                     WEIGGGEVRLFFPTESRALAEMLQARDPMERLRNIASRILGQPVRVCVKLEDVPVTGT
                     PSSGSGGSNGSSGTRELRAKFEQDPIVRAMMERFGGQISDVKRRGEE"
     gene            complement(8964..9062)
                     /gene="ffs"
                     /locus_tag="VG272_06700"
     ncRNA           complement(8964..9062)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VG272_06700"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(9275..10639)
                     /locus_tag="VG272_06705"
     CDS             complement(9275..10639)
                     /locus_tag="VG272_06705"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWF13407.1"
                     /translation="MTVCVLDDEPAMVEMLQESLRCLGFSSIGTSDPHYALELIEQSR
                     VRVVMSDIKMPGMDGLKFLEQAIQRDPGVYVMLMTGYYSIDNAIAAVKRGAYDYVPKP
                     VDRMRLKKSLDELSESFHKRKRIRSLEQQLLSDLEFHGIVGRSTAMVEVFEMVRKVAR
                     HYTNILLIGATGTGKELIAKSIHQMSPVSQQRFAVCNCSAMVDTLLESQLFGHVRGAF
                     TGATDTRPGLFEYANGGTVFLDEVGETSLAMQAKLLRVIQNREIQRVGSPEVRHVDVR
                     LIAATNRDLRAEVLAGRFREDLFYRLSTIQIRVPNLADRLDDIPLLIQFFLKKYNEAY
                     AKQIKGLTRRAQTVLYQHAWPGNVRELENVISCACLTSANDFVDVDDLPENLQKPSAS
                     SGRGGDAWRPVPLEEVRRQHIRRVLDACEGNRVRAAQMLGIGRTSLYRFLKRAQKKSA
                     TSGA"
BASE COUNT         2300 a         3019 c         3070 g         2656 t
ORIGIN      
        1 gcacaagcct gccattcctt gatcgttttc taaccttgtg gatttttttg gcaatggcca
       61 tcggagttgc gctgggccat tttattccac aaacggcgac acttgtacaa gattttcaga
      121 tagggacaac gaacattccc atcgccatcg gccttatcat tatgatgtac ctgcctttgg
      181 caaaagtacg gtatgaaaaa cttggtgaag ttttccgcaa tcgccgcgtt cttgggcttt
      241 cgcttctaca aaattgggtt attggaccaa tcctgatgtt tttcctcgcg gttacctttc
      301 tctgcgggga accagcctat atgcgtggcc tcatcttgat tggactggcc cgctgcatcg
      361 caatggtgat cgtgtggaac gaattagccg ccggcgacac cgactacgca gcaggtttag
      421 tcgcattcaa cagtgtcttc caagttctct tctacagcgt ttatgcgtgg gcgtttatca
      481 ctgtgcttcc gaagtggttt ggcctggagg gaagcgttgt ccatgtggga atcggccaga
      541 tcgcgcaaag tgtttttttc tatttgggag ttccgtttct tgcggggatt atcacgcgcc
      601 tgctgttttt gcgcgccaag ggagaggaat ggtatcgaac ccagtttatt tctcgcatca
      661 gcccagtgac cttggtggct cttctgttta cgattctcgt gatgttcagc ctcaaaggtg
      721 acttgatcgt tcgcctgcct ttggacgtgc ttcgcgttgc tctgccactg ctcatctatt
      781 tcgcgctcat gttcctcgtc agtttctgga tggggcgcaa gcttggcgcg gattactcga
      841 aaaccgcgac gctatccttt accgctgcga gtaacaattt cgaactggca atcgctgtag
      901 cggtggctgt tttcggcctc aattcaggag aggcatttgt tggagtcatc ggtcctctcg
      961 tcgaggtacc cgcgttgctt ggacttgtga atgtgtcgct gtggtttcgg cgacggtatt
     1021 tcgcataaaa ctgtcactca gcagaacacg tttcgaatcc tgccttgaat gtcctgccat
     1081 ccaacgcgat actgggcgca tgcctgaaag tgcgtctcaa tccgctcttc gcgttagaca
     1141 actccgcaaa gcttacaagg atgtgcttgc ggtcgatggg ctgaaccttg aagtgcatgc
     1201 gggggagtgt ttcggcctgc ttgggccgaa cggggcgggg aagacaacta cagttgaaat
     1261 ttgcgaaggc ctgacggatc cggatgcggg agacgtcgaa gtgcttggaa tgcggtggag
     1321 ttcggatgcg gcgcaacttc gccagcgcct cggtattcaa ttgcaggaca cgcagctttc
     1381 cgaaaaactt acggtgcatg aaacggtgcg cctgttccgc agcttttttc agcagggtgc
     1441 ggatgctact gacgtgattg cgcgggtgca gctcgaagaa aaacaaaatt cccgcgtggg
     1501 ggacttatcc ggcggacaga agcagagatt ggcgctggcc tgtacgctgg tcggcgatcc
     1561 cgattttctt tttctcgatg agcctacgac tggccttgac cctcaagctc gccgacagct
     1621 ttgggaattg attgaagaat ttaaagagtc cgggcgcacg attctgctga ccacgcatta
     1681 catggatgag gctgagcgcc tttgtaaccg cgtggcgatt gtcgatcacg ggaaagaaat
     1741 cgcgctgggc tcgccacgcg aattgattgc ctccacttgc gccgagcatg tggttgaatt
     1801 tacggcgggg agtgcttccc acgcgctcga tgtatccgcc ttgcgacgca tcgaaggcgt
     1861 tcgcgaagtt cgcacgcaga aggatgccgt gcttttgcag gtgagcgaac ttcacactgc
     1921 cgttcccgct ttgctcgcgg agttgacacg ccagaatgtt ccgctcactg agtttcggac
     1981 tcactcggcc acgctggaag atgtattcgt ggctttgacg ggacggcatt tgcgcgatga
     2041 gtgaaaacac aatttccgaa gttcgcgccg ctacggagaa gaaatctccg cgcgcaaatt
     2101 cgttcgcgga tcatccgctt gtgcaactca cccttgtgcg cttcctcgaa ttcatccgcg
     2161 aaccggaggc tctattctgg gtgtttgcat ttcctgtttt gctggccgca ggactgggta
     2221 ttgcgtttcg aaacaggcct gccgacgtgc ttaaaattgc ggtggtctct cccgggctcg
     2281 cgcaatcttt gcagcgagaa aaacttctcg aaatacaaca gctcgatgag agtgtcgccc
     2341 aagaggcctt gcgcacgggc aaagtggcgc tgctcgcatt gcccggatcg gctggcacgg
     2401 ttgaataccg gtatgacgat acgaatccgg aaggtcgcgc ggcgcgcctg cttgcggatc
     2461 gcgcgatcca gagagcggca gggcgggcag atcccgttgc taccagcgac cagttaatgc
     2521 gcgaacccgg ttcacgttac atcgacttcc tgatccccgg tctcctggga atgaatttga
     2581 tgggcagcgc gatgtggtcg attggttttg cgatcgtcga tgcgcgacga aaaaaactct
     2641 tgaagcgtct ggtggcgagc cccatgccgc gcccctacta tttaatttcg ttcctccttt
     2701 cgcgaatttt gatgctggtg ttggaagtcg gagttcttgt cggtttcggt gcgctgtttt
     2761 ttcacgtgcc cgttcgcggg tcgttgctca cgcttgccac tttgtgcgtg ttgagctcgc
     2821 tttcattttg cgcactggga cttttgctgg cttcacgcgt aaaaacgatt gaggcggctt
     2881 ccggcctgat gaacgtggtg atgatgccca tgtggatcgc gtccggcgtg ttcttttctt
     2941 cgcagaaatt tcccgggttg atgcagcctt tcatcaaagc gcttccatta accgcagtga
     3001 tcgatgcgct gcgctcgaac atgctgcaag gcgcgaactt atcgcagatg atgccgcaag
     3061 tagggaccat tctcttctgg ctggtgattt gttttgcact ggcactaaag atctttcggt
     3121 ggaggtaggg ttttatgtgg cataggcttc agagtctgtg tgagaactac aaaattgaag
     3181 cttcagcggc taaagccgca ctcattttgc aggacttatg tcgtggctaa agccacgacc
     3241 cacaaagatc ctgcagttct cacacagact cttcagcctg tggattttag tccttacttg
     3301 aactaatccc cacaggctga agcctatgcc acgtaagtcc taacccggaa tcagacgatg
     3361 cttgaaggtg cgctggccct tgttgtagct ggcgacggtc tggcctttgc caaacagttt
     3421 gtatttgtaa atggtgaggc cgtcgaggcc cacagggccg cgcgcatgca attttcccgt
     3481 actgattccg acttccgcgc cgaggccgta gcggaaacca tcagaaaatc gcgtggaagc
     3541 attttgaaaa acgttggccg catcgacgaa ttccataaag cgggcggcga catcgtggct
     3601 ttcggtgacg atggattccg tgtgccgtga gccgtatttg ttgatgtggt cgattgcctg
     3661 attcactccg tccacaactt tgacggaaac gataaggtcg gaatattcct ttttccaatc
     3721 ttcttccgtg gcgggaataa ttccgcgcgc ttttgtcaac gctaaagtcc gcgcgcagcc
     3781 gcgtacttcg acgccggcgt tttgaaattc ctcaatcatc agcggcagaa acagcggcgc
     3841 aatttcctga tgcaccagca aagtttccat ggcattgcac acggcagggt actgaacctt
     3901 ggaatcgtag gacacgtcga ttgctttttg aacatccgcg tcgctgtgca cgtaaacgtg
     3961 gcagatgcct tcaccatggc cgagaacggg gattaggctg ttttgcatga cgtaagttac
     4021 aaattcgttc gatccgcgcg gcacgatcag atcgatgtcc tcgtgcattt tcagaatctc
     4081 cgctacatcc tggcgggtgt ggatcagcac cacggagtcg acaggaatgt cggagaaggc
     4141 actcagcgcg tcacgccaga tcgagacgag caccgcgttg gtttccgccg cttccgcgcc
     4201 gcccttgagg caaaccgcgt ttcctgattt cagcgccagc gaagaaattt gtggcacaac
     4261 atccgggcga gactcaaaca cgacgcccac aactcctagc gggcaagttt ccttgtgcag
     4321 gacgagatcg tcgtccaact ccattgtgga aaggcgccgg tgcaggggat cgggcagcct
     4381 ggcaacatcg cgcactcgcg cagacatatc ggcaatgcct cgttccgtgg ttcgcaaacg
     4441 cgcgaacatc gcgcgggaaa gttcgccgag ctcgacggcc ttttgggccg cggcgcagtc
     4501 cttgtcgttc gcagcctgaa tttccgctgt gcgcgcttca atcgcattgg ccgcagcgat
     4561 cagggcttcg tttcggcgtt caccggaaag cgtcgcgaga acatgcgcag ccttgcgcgc
     4621 acgcttgatc gtgtgccgag cgtcgaaagc ttccgcttgt ttttgtacgg tgctcatttg
     4681 cgatccagta gaacgatatt atcgcgcttt acgaggacac ttgcacgctt gcttgtttgg
     4741 ccggggacat cggttacgcg cgaaccgttt ccgatcaatc cttgcgcatc gccgcgcgaa
     4801 taatccgtga tgccgcgcgc gaattccact ccctcgccgt ccacgatgct cacaacttct
     4861 gccgcgtcaa attctccttc gacgcccgtg acacctgagg ccagcaagct cgccttgcca
     4921 ccagtgagcg cttcgcgcgc gccggcattg acgatcacgc gcccgcgaac tcccgcagca
     4981 tacgcaatcc agcgccgctt gcccgccatg cgcgttttgg aaacaaacac ggtgcccgcc
     5041 ggctcaccat taaaaacttt catcagcgca tccggcttgg tgccattggt gatgacggcg
     5101 acgccgccag cctgcatcgc aatttccgcc gcttctagtt tcgttagcat gccgccgcgc
     5161 cctccttcgg aggggccaag agcgagcgct ttcaattcgg aattgatctc ttccactagg
     5221 ggaataattt ttgcatccgc atcgatagcg ccagacggcc cgagctgcat caatccatct
     5281 acgtccgtaa gcaggaccag cgcgtcggcc tcgacgtggc tcatgacgag ggcggcaagg
     5341 cgatcgttgt cactgaagat gcgctcgccc gcgcggatgt tcagataatc cagctctgct
     5401 gtcgagacgg tatcgttttc attcacgatg ggcagcacgc caagtttcaa gagcgcttcc
     5461 atcgtttcgc gcaaattgga gtagcgggtg cggtcgacga agtcgccttc tgtgagcagc
     5521 acttgcgcaa tgtgtacgcc gaattgttga aacagttttt cgtattcatg catcagcaaa
     5581 ctttgtccca ccgccgcgca agcctgacgc attaccatgt cattcaagcg gtcgcgatgc
     5641 agaccaagac ggccccgccc cagtccgacc gcgcccgaag acacgagcac aatttggcgg
     5701 ccatcttttt tcagttgggc gatgccgcgt gccacgggct caagctgcgc gatgttaaaa
     5761 tgcccgtcgc tgtctgtcac gatacccgta cctagcttga tgaccgcgcg gcgcgatgca
     5821 ctcaggcgct tctgctgttc cgtctctgaa gtctgatttt ccacggggtc ttactttatc
     5881 atcgagcagg gcatgttcaa agatggtgtg ggttcggcgt ttggtttacg tggcagaggc
     5941 ctacaaatcg cggcggcctt ccatagcttt catcatggtg acttcgtcgg cgtattcgag
     6001 gtcggcgcca acgggaatgc cgacgccgat gcgtgtgacg cgcacgccga gaggtttgag
     6061 aagcttagaa agatacatgg ccgtggcttc gccctcggcg ttgggatttg ttgcgataat
     6121 aatttcctcc accacgccgc ccttgaggcg ttcgatcaaa tttttcagat gcagctgatc
     6181 agggccaacg ccacgcagcg gcgcaagcga tccgccgagc acgtgataca tgccgttgta
     6241 ctggcgcgtt ttttcgatgg ccatgatatt gtgcggttct tcgacgacgc aaatcgtttt
     6301 gcggttgcgc gtcgaactcg tgcaatagag gcatggatca acatccgtga tgttgttgca
     6361 cacggtgcag acgcgcatgt tcatcttggc atcgcggatg gcatcggcca gcgctagcgc
     6421 gttttccgaa tcggtgcgca gaatatgaaa cgccaggcgc tgcgcagtct tcgcgccgat
     6481 gccaggcaaa tgcttcagct gatcgatcag acgttctaca ggctcggcga aatcaggcat
     6541 gggaattttt ccgtgacgaa gtgaggcggg gcacagattc cgaattcact cgtgatgtag
     6601 gcttcaaaaa aaattctcaa agggcgcggc ttaaaacaac ccaggaattt tcagtccggc
     6661 cattcctgga attgatccgg tcagattttt catctggctg gtcagttctt catcgatgcg
     6721 gcggctggct tcattcacgg ccgagcgcac caaatcctgc aacagttcct gatctttact
     6781 cgcaaaaatt tccgaatcga tgtgaacatc ggtgagttgc ttctggccat tcatcttgac
     6841 ggtgaccata ccgccgcccg ccgaagcttc cacgtgcacg gtttcgacct gcttttgcaa
     6901 gccctcctgc atttgacgaa actgcgtgag aatctgctgt agcgccttca cttccatggt
     6961 ctactcctcg ccccggcgtt tcacgtcgga aatctggccg ccgaatcgtt ccatcattgc
     7021 gcgcacgatc ggatcctgct cgaactttgc gcgcaactca cgtgtgccgc ttgaaccgtt
     7081 tgatcctcct gaaccgcttg aaggagttcc ggttaccggc acgtcttcaa gtttaacaca
     7141 gacacgcacg ggttggccga ggatgcggct ggcaatgttg cgcaaacgtt ccataggatc
     7201 acgggcctgc aacatttccg cgagtgcgcg gctttccgtc gggaaaaata atcgcacttc
     7261 accgccgcct atttcccagt gcgatgcgtg tacgacgagt tcgcccaaaa atttttcctg
     7321 cgctacgatt gcgcccttga tggaattaat tttttcttcc aaaggtccag agccaaaggc
     7381 gtcaggccgc gcaaccgtgt cggttgtcac agcaggtggg ggagcaggtg tcgcgtcaat
     7441 tggtttcggc gagaaattta tttcctcgcg cttggcggtg aagggaactg tcgtttcgac
     7501 gcgtgcggcc tgtgttttcg caggcgctgt agcggttgcc gctccctgag atattgccgg
     7561 gctttgccat gtggcagaga ccgaggaagc cggcgcagag ctcgaacgcg cgggagccgg
     7621 ggtttgcggc agcgcgctcg aagagcgagt tggcaccgag ttcgcctgcg tgccgttatc
     7681 aagatcggca agaagttctt cgagcggcgc gaggcgcgcg gcattcacca tgcgcaacag
     7741 gcccatctcc agatgcaaac gcggatcagg tttcctgcgc acatcatcat ccgtgtgcag
     7801 caggatttga aaaaagcgag tgagatcttc ttcgctaaaa aattcggctg cttccttcag
     7861 acgaggccgc tcatcgacgg gcgcggctat caattccgaa tcggcgccgc aaactcgcgc
     7921 gacaagcaga ttacgaaaat ggccaatcgc ttcgcggcaa aaatgttgga gattttgtcc
     7981 ttcggccagc agtctgtgca cgagcgagag aacacgttcg gcagagcgtt cttcgatcgc
     8041 gtgcgtcaat tcatccagaa tttctccagg cacaacgccg agcagttcgc gaacctgcgc
     8101 atcggtgatt gtcgtgccgc aatatgcgcg cgcctgctcc agtagcgaga ggccgtcgcg
     8161 tacgcttcct tccgcggcac gagccatcac ggcgatggcg ccggactcag cggttagtcc
     8221 ttccttcgcg caaatatcgt tcagcgcatc ggaaatttcg gcgaacgaaa gtgctcggaa
     8281 gtgaaaatgt tgcgaccgcg aacgaatcgt gtccgcgaga ttttccggtt ccgtggtggc
     8341 catcacgaaa ataactttgt cgggaggttc ttcgagcgtt ttcagaagcg cgttcgaggc
     8401 ttccccagta agagcgtggg cctcatcgag gatgacgact ttgtagcggc cgccggcggg
     8461 cgcgtagcgc accatctcgc gcaattcgcg aatctggtcg atgccgcgat tggaggcagc
     8521 gtcaatttcg ataacatcga gagaatttcc ctggctgatt tcacggcagg agccgcattc
     8581 gttgcagggt tccgcggcag ggcccttcgc gcaattcagc gctttggcca gaatacgcgc
     8641 cgtagttgtt ttgcccacgc cgcgcgcgcc ggaaaaaata tatgcatgag caacgcgtcc
     8701 ggattgaatt gcattggaga gagtgcgcgt gacatgctgc tgcccaacca cgtcggcaaa
     8761 cgtttgaggc cgccactttc gcgcgataac ctggtagctc attccgtatg aacccagccg
     8821 ttataaaaat tcatccccgt attgtaactc gcctcttcag aggtgagggt ttagatgttc
     8881 tattgcctaa acccccgcct ctgaagaggc gggctacagc aactgtatcg ccgaagaatg
     8941 attccgatac atttagatga gaggaacttc gggtactccg cggcacacga agcgatcccg
     9001 gtaccgttgc tcccttccgg gcctggcggg gttcgcggga cttcgtcgca cagagcccga
     9061 agttccacgc atcggcagtc gctccactcg gaagcggctg ccagagcagt ctagcacagc
     9121 aacgcgaatg aaaaaagaaa gcaggttgca ctgaacaaaa tgcgaggctt gactgaggag
     9181 atttaatttc gtggcgtgcc aaaattgaaa gctgtccggg tttgaaccaa acgaattcaa
     9241 acccgccagc cgcaataagt atatagattt tttgctaggc tcccgaagtc gccgattttt
     9301 tctgggcgcg tttcaggaat cggtacaaac tcgtgcggcc aatgcccaac atttgcgcgg
     9361 cgcgaacgcg gttgccttca caggcatcga gcacccgacg gatatgctgg cgtcgaactt
     9421 cctccagcgg gacaggcctc cacgcgtctc cgccgcggcc cgaggacgca ctgggctttt
     9481 gcaaattctc cggcaaatcg tcgacatcga cgaaatcgtt tgcggacgtt aagcacgcgc
     9541 aggaaattac gttttcgagt tcacgcacgt ttccgggcca tgcatgttga tagagcaccg
     9601 tttgtgcgcg ccgcgtgagg cctttgatct gcttagcgta agcctcattg tattttttaa
     9661 gaaagaattg gatgagcaaa gggatgtcgt ccaggcgatc ggctaaattg ggtacgcgga
     9721 tctgtatggt gctgagccgg tagaataaat cttcacggaa tcggccagcc aaaacttccg
     9781 ctcgcaaatc gcgattggtg gcagcgatga gccgcacgtc cacgtgacgc acttccggag
     9841 aaccgacacg ctggatttcg cggttttgaa taacacgtaa caatttggct tgcatggcca
     9901 gcgatgtttc tcccacttcg tccaggaaaa ccgttccgcc attggcgtat tcaaacaggc
     9961 ccgggcgagt gtccgtggcg cccgtaaatg cgccccgcac atgcccaaac agctggcttt
    10021 cgagcagggt atcgaccatc gcggagcaat tgcagacggc aaaccgctgc tgcgatacag
    10081 gactcatctg gtgaatcgac ttggcgatga gttccttgcc tgtgccggtg gcgccgatca
    10141 gcaaaatgtt ggtgtaatgc cgcgccacct tgcgaaccat ttcaaagact tccaccatgg
    10201 ctgtacttcg gcccacaatt ccgtggaact cgaggtcgct caacagctgt tgttcgaggc
    10261 tgcgtatccg tttgcgtttg tgaaaagact cagaaagttc gtcgagagat ttcttaaggc
    10321 gcatgcggtc aaccggttta ggaacgtagt cgtaggcgcc gcgtttaacc gcagcgatgg
    10381 cgttgtcaat ggagtagtaa cccgtcatca gcatcacgta aacgccggga tcgcgctgaa
    10441 tggcctgctc aagaaattta aggccatcca tccctggcat ttttatgtct gacatcacca
    10501 cgcgcacgcg gctctgctcg atcaattcaa gcgcgtagtg cgggtcactc gtgccaatgg
    10561 aactgaagcc gaggcatcgc agtgattctt gtagcatttc caccatggcc ggttcatcgt
    10621 ccaagacaca tactgtcatg gcgtcttccg aactgcctcg aataaactgc cctgcatgag
    10681 atggtgccgt caaagtatcc atgatgaatc attggcacgg gtagtgccaa aaccatcgac
    10741 aaaataatgg agaaaggcaa atccttagtt aagtgttgag tgcgatcgag ggttgctgta
    10801 agtcttctgt gaacaacttg ttgagcggag aagtacggga ggtctgttta tgcaggcgaa
    10861 atggcgacac gatattctgc ccaatcttcg aagattgagt ttgaaacgtt gtcccgaatt
    10921 ggaatgaatc aatacgcatc tgtgccaaat ttgaattaag ccctacttaa caccagctta
    10981 attcggaact cccccatcca tctcgctcaa gtaggcgaaa aactcgaatt cttcgctggg
    11041 atacc
//
LOCUS       DASZMB010000147         5253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3995982, whole genome shotgun sequence.
ACCESSION   DASZMB010000147 DASZMB010000000
VERSION     DASZMB010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3995982"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            466..1416
                     /locus_tag="VG272_06710"
     CDS             466..1416
                     /locus_tag="VG272_06710"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HWF13408.1"
                     /translation="MKITILDDYFDTIRTLPCFEKLKKHEVQIWNDHVQDVDKLAERL
                     KDTEVLVLIRERTKIQAPLLARLPNLKLISQRSVYPHIDIDACTKLGVVVSSSQHAGT
                     PCYAAAELTWGLILAAVRQIPQQMASLQAGNWQIGVGDTLRGKTLGIYGYGRIGSTVA
                     SYGKAFGMKVLAWSGKDALERARQDGYVAADSKEVFFEESDVLSLHLRLYNATRGIVT
                     AADLARMKPTSLLVNTSRAPLIEPGALVNALQKGRPGMAAVDVFEEEPLRDANHPLLT
                     MKNVVCTRILDMSRAMNMTSSSLKFSTRSRLSGWARQLML"
     gene            1578..1946
                     /locus_tag="VG272_06715"
     CDS             1578..1946
                     /locus_tag="VG272_06715"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HWF13409.1"
                     /translation="MSAARRNAISAVRPAPEAPGESARFSATQAKNEFARALELALQG
                     GSVVITKHDAPKAILISMDEFNKLTRAAEYNLNTLSAEFDTMLDRMQTPKARSAMEAA
                     FSASPKELGKAAVSAARKRG"
     gene            1939..2589
                     /locus_tag="VG272_06720"
     CDS             1939..2589
                     /locus_tag="VG272_06720"
                     /inference="COORDINATES: protein motif:HMM:NF018154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HWF13410.1"
                     /translation="MAKTAQNQCIYVLAGTNGAGKSSIGGAMFRQHGAEYFNPDEAAR
                     RILSENPAISQEKANSAAWNEGKRLLERAIDKRYIFAMETTLGGHTIAALLEKAALAG
                     IEVRVWYVGLESPELHIARVHTRVQKGGHDIPEAKIRERYARSIWNLIQLFPKLTEVF
                     VYDNSEEADPHSGRAPNPKLILHARKGKTVATCDLKQTPNWAKPILMAALHAFPNR"
     gene            complement(2593..3861)
                     /locus_tag="VG272_06725"
     CDS             complement(2593..3861)
                     /locus_tag="VG272_06725"
                     /inference="COORDINATES: protein motif:HMM:NF017019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HWF13411.1"
                     /translation="MIRHSSNSAHVLPFVSHAFLLAAFLLAMAAAPASAQVKPKIRAI
                     TAFIRLDPTQYKQQITETLGMLRKSEARFDLAGYQVQTIRITTQPFTEYMHGKSKQEI
                     LAFFHGLDDVAKRENILIGIGPALMSDSDDPGQAQILAEILSGTSQIRGTIVVAGNDG
                     IHWKSVHAAAEVMKYLEDHTDHSVGNFYFAAIANVPAYSPFYPASYHQGNGHQFAIAL
                     ESANVVAAAMATQRDAEATRQAIIAELGMHASVIEDIAGKIDSESGWTYLGIDLSPAP
                     HKEVSIASAMTGFTGGHFGTSGTMTAAATITSALRDISVKKVGYSGLMMPVMEDTRLS
                     QLWSEGAISIDQLLAYSAVCGTGLDTIPLPGDTSTQQLERIIGDMASLSVKWTKPLSA
                     RLLPVAGAKPGDRTAFDDPNLINTIIQPLP"
     gene            complement(3899..5071)
                     /locus_tag="VG272_06730"
     CDS             complement(3899..5071)
                     /locus_tag="VG272_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWF13412.1"
                     /translation="MPAFPGVDFLDFDSLLNDEELLARKTARQFVDEHVIPIIERHNR
                     ESTFPLHLVPKLGEMGFFGANLEGYGCAGMSNVAYGLVTQELERGDSGLRSFVSVQSA
                     LVMYPIFSYGSPGQKDKWLPRLQSGKAVGCFGLTEPQFGSNPGGMLTRAVRRGDRYVL
                     NGEKMWITNGSLADVALVWAKCEDEKIRGFLIDKGTPGFKAWDVHGKLSLRASITSGL
                     AMTDCEIPAENVLPGVAGMKGPLSCLTQARYGIGWGAIGAAMACYDTALQYAKTRKQF
                     QNKPIASHQLVQDKLVFMITEISKAQLLALHIGRRKDQGKVEPSHISMLKMNNVWMAM
                     ETARKARDILGANGIVDDYCVMRHMNNLESVYTYEGTHDIHRLVIGERITGIPAYF"
BASE COUNT         1228 a         1468 c         1416 g         1141 t
ORIGIN      
        1 ggtcagcctt gtggccgtcg cccagcccca tcgtctttgc cgattcaacg gccgcttgtt
       61 cgacaacgcg caaaaattct agactcagtt cgatttccat cgaagctccc gcctatattt
      121 atttttagat atggcgcgca gacggacgca aactcttgcg cggcacggtc aaaacataac
      181 ctatcctaaa ctaagcgggc cgtgtgaacc tagtggaaca acttttattt tgtgcttaaa
      241 atttcgagga catgttgggg aggaaacaca aaccgcagga ccgagccgta aaggaggagt
      301 caagtgaagt ggatacgtta acaaccctcg gttgattgac aatctgaatc aggctcaact
      361 caacttcggt catttagtcg ttgggaatca cactcgttga gatatattca ggttaggttt
      421 tggaaaaatt cttgatgcta aaaatgatcc gattgcgagt ccaggatgaa gatcactatt
      481 cttgacgatt actttgacac aatccgaacg cttccttgct ttgagaaact taaaaaacac
      541 gaggtccaga tctggaacga tcacgtgcag gatgttgaca agctggccga gcgcctgaaa
      601 gacacggaag tgctcgtgct catccgcgaa cggacgaaga tccaggcacc cttgctcgcg
      661 cgcctgccca atctgaagct tatcagccag cgcagtgttt atccgcacat cgatattgat
      721 gcctgcacga agcttggcgt ggttgtctcg tccagccagc atgccggcac gccgtgctac
      781 gcggcagcgg aattgacgtg gggcctcatt cttgccgccg ttcgccagat cccgcagcaa
      841 atggcttcgt tgcaagcagg gaattggcaa attggcgtgg gcgatacgct gcgcggtaaa
      901 acgctgggga tttatggtta cggacgaatc ggcagcaccg tcgcgagtta cggcaaggct
      961 ttcggcatga aagtcctcgc ctggtcgggc aaagatgcgc tcgaacgcgc gcggcaggat
     1021 ggctacgtag cggcagacag caaggaggta tttttcgaag agagtgacgt gctctccctc
     1081 cacctgcgcc tttacaacgc aacccgggga atcgtaaccg cagcggattt agcacgcatg
     1141 aaaccgacat cgcttcttgt gaataccagc cgcgcgccgc tcatcgaacc cggcgcgctg
     1201 gtaaacgctt tgcaaaaggg ccggccagga atggccgcgg tggacgtctt cgaggaagaa
     1261 ccgctgcgag acgcgaatca tccgctgctc acgatgaaga atgtcgtgtg cacccgcata
     1321 ttggatatgt cacgcgcgat gaatatgacc tccagttctc tgaaattttc gaccaggtca
     1381 cggctttctg gatgggcacg ccaattaatg ttgtaaatcc cgcagttttc ggtccttcaa
     1441 agaaatagat tccagtggca acagtagtaa tgtggcgctg ggcttcaagt ccgggagcca
     1501 tttttacgcg cgtcccttca ttttttcata ttgaccagaa tagctaaaat agctaaaatg
     1561 gctattagga gaaacctatg agtgctgcgc gtagaaatgc gatttccgct gtgcgtcctg
     1621 ctccagaggc tcccggcgag tctgcgcgtt tttcggctac gcaggccaag aatgaatttg
     1681 cccgcgccct cgaattggcc ctgcagggcg gcagcgtcgt catcaccaag cacgacgcgc
     1741 cgaaagccat cctaatctcc atggatgagt ttaataagct cacgcgcgcc gccgaataca
     1801 acctcaatac tctcagcgcc gaatttgaca ccatgctcga tcgcatgcag acgccaaagg
     1861 cacgctccgc gatggaagct gcattctccg cgtcgcccaa ggaacttggc aaagccgcag
     1921 taagcgctgc gcgaaagcgt ggctaagaca gctcagaatc agtgcatcta cgttctagcc
     1981 ggcacgaatg gcgcgggcaa aagcagcatt ggcggggcaa tgttccgcca gcatggcgcc
     2041 gaatatttca atccggacga agctgcacgc cgcatccttt cagaaaatcc cgccatctct
     2101 caggaaaaag cgaacagcgc ggcatggaac gaagggaagc gcctactcga acgcgcaatc
     2161 gataagcgat acatctttgc gatggagaca acgcttgggg ggcacacgat tgcggctctt
     2221 ctcgaaaaag cagcacttgc agggatcgaa gtacgtgtct ggtacgtggg tctcgaaagc
     2281 ccggagctgc acatcgcgcg ggtgcacacc cgcgtgcaga aaggcggaca cgatattccc
     2341 gaggccaaaa ttcgcgaacg ctacgcacgc agcatctgga atttaattca gctgtttcca
     2401 aaattaactg aggtttttgt ctacgataac agcgaagaag ccgatcccca ctcaggccgc
     2461 gcacccaatc ccaaactaat tctccatgcg cgaaaaggga agaccgtggc gacgtgcgat
     2521 ttgaagcaga ctccgaattg ggccaagcca atcctgatgg ccgcgctgca cgccttccct
     2581 aaccgataaa aatcagggca gcggttggat aatggtgttg atcaggttcg gatcgtcgaa
     2641 cgccgtgcga tcgccgggtt ttgcgccagc cacgggcagt aaacgcgcgc tcagcggctt
     2701 ggtccatttt acggagaggc tggccatgtc gccgatgatg cgctcaagct gctgcgttga
     2761 ggtgtcgccg ggcagcggaa tggtatcgag ccccgtgccg cagaccgcgg aataggcgag
     2821 caattgatcg atggagatcg cgccctcgct ccacaattga gaaagtcgcg tgtcttccat
     2881 caccggcatc atcagccccg agtagcccac cttctttacg gaaatatcgc gaagcgcgga
     2941 agtaatcgtt gcggccgcag tcatcgtgcc gctggtgccg aaatggccgc ccgtgaaacc
     3001 cgtcatggcc gatgcaatgg aaacttcctt atgcggtgcc ggggaaaggt caatgccgag
     3061 gtaagtccag cccgattcgc tatcaatctt gccggcaata tcctcgatca cgctcgcgtg
     3121 cattcctaac tcggcaataa ttgcctggcg cgtggcttcc gcatcgcgct gagtggccat
     3181 cgcggcggca accacattgg ccgattccag cgcgatagcg aattgatgtc cgtttccctg
     3241 atggtaggac gccggataaa acggcgaata agccgggaca ttcgcaatgg ccgcgaagta
     3301 aaaattgccg acgctgtggt ccgtatgatc ttccagatat ttcatgactt cggcggccgc
     3361 gtgcacggat ttccaatgga ttccgtcatt tccggccacg acgatcgtcc cgcgaatctg
     3421 cgaggttccg ctcaaaattt ccgccaggat ttgcgcctgc ccgggatcgt cactatcaga
     3481 catcagagcg gggcctatcc cgatcaaaat gttttcgcgc ttcgcgacat cgtccaggcc
     3541 gtgaaaaaac gccagaattt cctgtttgga ttttccatgc atgtattccg taaagggctg
     3601 cgtggtgatg cgaatcgtct ggacctggta tcccgccaga tcgaagcggg cctccgattt
     3661 acgcaacatg cccaaagtct ccgtgatctg ctgtttatat tgtgttgggt ccagccggat
     3721 gaacgcggtg atggcgcgaa ttttcggctt cacctgcgcg gatgcgggcg cggcggccat
     3781 cgcaagcaga aatgccgcaa gcaagaatgc atgagaaaca aatggaagga cgtgtgccga
     3841 attgctggaa tgacgaatca tcaagttctc ctcaaataag caagcccgcg atcactgctc
     3901 agaaatacgc gggaatgccc gtaatgcgct cgccgatcac caaccggtga atgtcatgcg
     3961 taccttcgta cgtataaacc gactcgagat tattcatgtg ccgcatgaca cagtaatcgt
     4021 ccacaattcc gttcgcgccc agaatatcgc gcgctttgcg tgccgtttcc atggccatcc
     4081 acacgttatt cattttaagc atggaaatat gcgacggctc taccttgccc tgatcttttc
     4141 gtctgccaat atgcagcgcc agcagctgcg ctttggaaat ttccgtgatc atgaagacaa
     4201 gcttatcttg caccaactgg tgcgatgcga ttggcttatt ctgaaattgt ttgcgtgtct
     4261 tcgcgtattg caaagctgtg tcatagcaag ccatcgccgc gccaatcgct ccccagccga
     4321 ttccgtagcg cgcttgcgtc aggcaactga gcgggccctt catgccggcg acgccgggga
     4381 ggacattctc ggcgggaatt tcgcaatcgg tcatcgccag cccggatgtg atggaagcgc
     4441 gcagcgaaag ttttccgtga acgtcccagg ctttaaaacc aggcgtccct ttatctatca
     4501 gaaagccgcg aatcttttcg tcctcgcatt ttgcccacac gagggcaacg tcggcgagcg
     4561 atccattcgt gatccacatt ttttcgccgt tcaatacgta acgatcgccg cgccggacgg
     4621 ctcgcgtcaa catgccgcct gggttggacc caaattgcgg ctcggtgagg ccaaagcatc
     4681 ccacagcctt tccgctttgc aggcgcggca accacttatc tttttgtccc ggcgatccgt
     4741 agctaaagat cggatacatg acgagcgcgc tctgcaccga aacgaagctg cgaagtccgg
     4801 aatcgccacg ctccaattcc tgcgttacca ggccgtaggc aacgttggac attcccgcgc
     4861 agccgtagcc ttcgaggttc gcgccgaaaa atcccatttc gcccagtttc ggcacgagat
     4921 gcaaggggaa cgtcgactca cgattatgcc tttcaataat ggggatgacg tgttcgtcca
     4981 cgaactgccg tgcggtcttg cgtgcgagca actcttcgtc attcaggagc gaatcgaaat
     5041 ccagaaaatc cacaccggga aacgcgggca ttcaattctc cttatccaaa ttcgactgat
     5101 cgggcgaaaa aaaattgcct ggcgaagcaa agctctttgt atccatatat agaattgacc
     5161 gggcacggcg cgctcattct aacgcgccca gtttagcccc agcaagcgag tgctgcggta
     5221 agcttggtgt cgccggcggg tcgccggcga cac
//
LOCUS       DASZMB010000148         7127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_438883, whole genome shotgun sequence.
ACCESSION   DASZMB010000148 DASZMB010000000
VERSION     DASZMB010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7127
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438883"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /gene="tsf"
                     /locus_tag="VG272_06735"
     CDS             <1..417
                     /gene="tsf"
                     /locus_tag="VG272_06735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HWF13413.1"
                     /translation="EINCESDFVARTEDFQRLCHDVAMHIAALDTRFVRREDVTQEIL
                     DREREIYKAQARATGKPEAVAEKIVNGKMEKFYEENCLYEQHFIKDESMTVGEMVSQM
                     IHKLGENISIRRYTRFKVGESAATSTEEPSAAVSAS"
     gene            536..1294
                     /gene="pyrH"
                     /locus_tag="VG272_06740"
     CDS             536..1294
                     /gene="pyrH"
                     /locus_tag="VG272_06740"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HWF13414.1"
                     /translation="MSKDDRTPNHGTSTPAPIYKRIVLKLSGESFQGSQGFGIDAETV
                     HAIAREVKEVNDLGIQMAIIVGGGNIFRGTRQKSLSIDRATGDYMGMLATVINGLALQ
                     DALEKQGVVTRLLSAIDMAQVAEPFIRRRAMRHLEKGRVVIFAAGTGNPYFSTDTAAA
                     LRAMEIKAHVILKATRVDGIYDADPEKVPGAKMFERITYLDVIQKGLAVMDSTAISLC
                     MDNRMPIIIFNMNTAGNIRRAVMGEKVGSTVTAE"
     gene            1312..1887
                     /gene="frr"
                     /locus_tag="VG272_06745"
     CDS             1312..1887
                     /gene="frr"
                     /locus_tag="VG272_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HWF13415.1"
                     /translation="MSVISNTKEAMAQARARMEKAVEDFRKELAGVRTGRASVALLDH
                     IRVDYHGTSMPVNQLGALSVPEPMSIVISPWDPGAVPLIDKAIRTSDLGLNPTNDGKV
                     VRVPIPPLTEDRRKDIVKHLHKVLENHRTAVRNIRRDIKEAVDKLEKEKKISQDEQKR
                     TLDELEKITHSETKKIEDLCAVKEKEILEVK"
     gene            complement(1902..2309)
                     /locus_tag="VG272_06750"
     CDS             complement(1902..2309)
                     /locus_tag="VG272_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWF13416.1"
                     /translation="MNLRYMLDTDICIYISKRKPPEVKARFDCLKPGQVVMSVITYGE
                     LYYGASKSNQRTKAFAELERTVRGIPIENLTSAVSEAYGTIRAALEKQGRVIGNNDLW
                     IAAHAMALDVTLATNNEREFLRVVGLSVENWTK"
     gene            complement(2306..2536)
                     /gene="vapB"
                     /locus_tag="VG272_06755"
     CDS             complement(2306..2536)
                     /gene="vapB"
                     /locus_tag="VG272_06755"
                     /inference="COORDINATES: protein motif:HMM:NF040493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HWF13417.1"
                     /translation="MSKARIFKSGNSQAVRLPKEFRFKVKEVDISRRGDEIILSEPKC
                     SLADAFDALASMPEDFMAEGRKDDPPQKRKNL"
     gene            3253..3636
                     /gene="rpsL"
                     /locus_tag="VG272_06760"
     CDS             3253..3636
                     /gene="rpsL"
                     /locus_tag="VG272_06760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HWF13418.1"
                     /translation="MPTFAQLVRSGRDQIRTKTKSPALQGCPEKRGVCIRVYTQTPKK
                     PNSALRKVARARLTNSIEVTTYIPGIGHNLQEHSIVLVRGGRVKDLPGVRYHIIRGTL
                     DAAGVENRKQGRSKYGAKRPKAAQK"
     gene            3688..4158
                     /gene="rpsG"
                     /locus_tag="VG272_06765"
     CDS             3688..4158
                     /gene="rpsG"
                     /locus_tag="VG272_06765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HWF13419.1"
                     /translation="MPRKGFVPRREVTTDSVYSSDLVQKFINSMMQDGKRSTAQRIFY
                     GAMDQIGQKTNGEALATFKKAIENVKPVLEVKTRRVGGANYQVPVEVNPFRRQSLAIR
                     WLLLYARTRSGKTMTDKLAEELLDAANARGGAMKKKEDVHRMAEANKAFAHYRW"
     gene            4293..6551
                     /gene="fusA"
                     /locus_tag="VG272_06770"
     CDS             4293..6551
                     /gene="fusA"
                     /locus_tag="VG272_06770"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HWF13420.1"
                     /translation="MARKAQLAETRNIGIMAHIDAGKTTSTERILFYTGVTYKIGSVD
                     EGTATMDWMEQERERGITITSAATTASWKRNAKEFLINIIDTPGHVDFTVEVERSLRV
                     LDGAVAIFDAVAGVQPQSETVWRQADKYHVPRLAFINKMDRTGADFEHALQTMRQRLA
                     AHPVPIQCPIGKEDLFIGVIDFLEEKAIIWLEETLGAKFEVMDVAKLWDPEFLAGRPD
                     VVAALKFCALDEAYYKERREKSVEYIAEHDDTLVEKYLNGEKITADELRASIRRSTMA
                     LKLVPVLAGSAFKNKGIQPLLDAVVDYLPSPLDVPPVEGTTANDEVELRRASDDEPFA
                     ALAFKIMSDPFVGHVTFIRVYSGVLKSGSYVYNATKQTRERISRLVRMHANKREEIDA
                     IYAGDIAACVGLKSVTTGDTLCDEQKTIVLETIEFPAPVISVAIEPKTKADQDKLGNA
                     MQRLAQEDPTFRVHSDEDTGQTIISGMGELHLEIIVDRMMREFGVQATVGRPQVAYRE
                     TIRKRAEAEGKYIRQTGGRGQYGHCIIEVLPLPSSAHENMHEMSTDELAILAKEISGG
                     GGKPRFDKEHRMLFIDKTVGGSIPKEFIAPIDAGIREAMENGILAGFEMVDIAVLLID
                     GSYHDVDSSEMAFKIAGSMAFKEACSKAKPVLLEPIMKVEVVTPEEYMPQVFGDLNAR
                     RCQIQGTESRGGANVIRVEVPLASMFGYATDVRSRTQGRASFTMHFSHYAEAPAAVAE
                     EVIAKSTGRATR"
     gene            6627..>7127
                     /locus_tag="VG272_06775"
     CDS             6627..>7127
                     /locus_tag="VG272_06775"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWF13421.1"
                     /translation="MAKEKFERNKPHLNIGTIGHIDHGKTTLTAAITKVLSKHNPKVA
                     FRAFDSIDNAPEERERGITIAVSHVEYETANRHYAHIDCPGHADYVKNMITGAAQMDG
                     AILVVAATDGPMPQTREHILLARQVGVPSIVVFMNKCDSVEDPELLELVELEVRELLK
                     LYQFPGD"
BASE COUNT         1736 a         1929 c         2006 g         1456 t
ORIGIN      
        1 gaaatcaatt gcgaatccga tttcgtcgcg cgcaccgagg atttccagcg cctgtgccac
       61 gacgtggcca tgcacatcgc cgcgctcgat actcgatttg tccgccgcga ggatgtcacg
      121 caggaaattc tggatcgcga acgcgaaatt tacaaagcgc aggcccgcgc caccggcaag
      181 cctgaagccg tcgccgaaaa aatcgtgaac gggaaaatgg aaaagttcta cgaggaaaat
      241 tgcctctacg agcagcactt catcaaggat gaatccatga ccgtcggcga aatggtcagc
      301 cagatgattc acaagctggg cgaaaacatt tccatccgcc gctacacgcg tttcaaagtg
      361 ggcgagagcg cagctacttc cacagaagag ccatcggccg ctgtttcagc gagctagata
      421 gttcgtgatc gaaaaacggt cgcggaattt gtcagcttat tgaacagcgg cactgaaaat
      481 tcgaatgcgg gccgtgcacg ccggataaat ctttgttaga atgcccgccg agagcatgag
      541 caaagacgac cgaacaccca accacggtac ttccactcct gcgcctattt acaagcgcat
      601 cgttttaaaa ctttccggag aatcatttca gggttctcaa ggttttggca ttgatgccga
      661 aaccgtgcac gcgattgccc gcgaagtgaa agaagtaaac gacctgggta ttcagatggc
      721 gatcatcgtg ggcggaggaa atatattccg cggcacgcgc cagaaatcgc tttcgattga
      781 tcgcgccacg ggcgattaca tgggcatgct ggccacggtg attaacggcc ttgccttgca
      841 ggacgcgctg gaaaagcagg gagtcgttac tcgcctgctc agcgccattg acatggctca
      901 ggtggccgag ccatttatcc gccgccgcgc catgcgccat ctcgaaaaag gccgcgtcgt
      961 aatttttgcc gcgggcacag gcaatccata tttttcgacg gacacggccg ctgccctgcg
     1021 cgccatggaa attaaggcgc acgtgattct gaaggccacg cgagtcgacg gaatttacga
     1081 tgccgatccg gaaaaagttc ccggggcgaa aatgtttgaa cgtattacct acctggacgt
     1141 aatccaaaaa gggctcgccg taatggattc caccgccatc tccctgtgca tggacaatcg
     1201 catgccgatc atcattttta acatgaatac ggcgggaaac attcgccgcg cggtgatggg
     1261 agaaaaagtg ggatccacgg tgaccgcgga ataaacgggg ggctcgaaaa aatgagcgtg
     1321 atttccaaca cgaaagaagc gatggcgcag gctcgagcgc gcatggaaaa agccgtcgag
     1381 gattttcgca aggagcttgc cggcgtgcgc accggccgcg ccagcgtggc tctgctcgat
     1441 cacatccgcg tcgattacca cggcacgtcg atgccggtga accagctggg cgcgctttcc
     1501 gtgcccgagc cgatgtccat cgttatttcg ccgtgggacc ccggcgccgt gccgctgatc
     1561 gacaaagcca ttcgcacttc ggacctgggc ctgaatccca cgaatgacgg taaggtcgtg
     1621 cgcgtgccga ttcccccgct gaccgaagac cgccgcaaag acatcgtcaa acacctgcac
     1681 aaagttctgg aaaatcaccg caccgccgtg cgcaacattc gccgcgacat caaggaagcc
     1741 gtcgataaac tggaaaaaga gaaaaagatc agccaggacg aacaaaagcg caccctcgat
     1801 gagctagaga aaatcacaca ctccgaaacc aaaaaaatcg aagacctctg cgccgtaaaa
     1861 gaaaaagaaa ttttagaagt gaaataaagc gggagggcgg attatttcgt ccagttttcc
     1921 acggaaagcc caacgacgcg aaggaactcg cgttcgttgt ttgtcgcgag ggtcacgtcc
     1981 aaggccatcg cgtgcgccgc gatccataag tcgttgtttc cgatgactcg tccctgcttt
     2041 tcaagcgccg cgcgaatggt cccgtacgcc tcggacactg ctgacgtaag attttctatg
     2101 ggaatgccgc gtaccgtcct ctcgagttcc gcgaaggcct tggttctctg attgcttttg
     2161 ctcgcgccgt aatacaactc tccgtaagtg atcacggaca taaccacctg gcccggtttc
     2221 agacagtcga agcgcgcctt gacttccgga ggtttgcgtt tggaaatgta aatgcaaatg
     2281 tccgtatcca gcatgtaccg caggttcata aattcttacg cttctgagga ggatcgtctt
     2341 ttcgaccttc cgccatgaaa tcctccggca tggaggcgag cgcgtcgaat gcgtccgcca
     2401 gactgcattt cggttcactc agaatgatct cgtcccctcg gcgcgaaatg tcgacttcct
     2461 tcaccttgaa gcgaaattcc tttggtaacc gcaccgcttg gctgttgccg gatttaaaga
     2521 ttcgcgcttt gctcatgcca tcacctctcc catacagtat atacaataaa tatatacgtt
     2581 tacaatcgtt aagatcggct gaagaacaca ggagcttcct accttgcctg ctgccactcg
     2641 tttgcgggtt ctacgactgc gggtttaaat tagcctatgt caactcaccg gctcacctgc
     2701 gatttttatt ttccattatc gattcaaaga ccgcacggtc gagacccgca tggtacaaat
     2761 tgagaacacg ctgatagaag tctaccttcg tccgttgtcg tcctttaatt tcaggctcat
     2821 ttcaggctct tgaggctcaa tatccgaatt ttaaatacat tgaaaaagat aaataaattt
     2881 gccgcaaata tttccaacag ccatcctagg atttttgttt gacataagtg tctgcattgc
     2941 ctatactgtc ggcttcgtca gggattgttt tgccctgtag tgaaagggat agtcttcaag
     3001 ccccggaaca aagaacgaag gatcggggta agaagagcgg ccggaatggg acgccagtcc
     3061 cgcaaagccg agtaaccctc tccgcttcga ccagaaagta gaatctgctc actggtatta
     3121 gtgcggggat tgctgttggt tgctccggat tcgccactat actctttcct gaatgaaacc
     3181 cacgacaccc cgtctggtca gaaatggcca tatggaaagt gaagtgaaat ctagctcgtt
     3241 tgagggagag ccgtgccgac gtttgcacaa ctggtacgat ccggtcgcga tcagatccgg
     3301 accaaaacga aatcacccgc gttgcagggc tgccctgaaa agcgtggcgt ttgcattcgc
     3361 gtatacaccc agacgcccaa gaagcccaat tccgcgctgc gtaaagttgc gcgcgcgcgt
     3421 ctgacgaaca gcatcgaagt caccacttat attcccggga tcggccacaa cctgcaggag
     3481 cactcgatcg tgctcgtgcg cggcggccgc gtgaaggatc ttcccggtgt gcgctatcac
     3541 attattcgcg gaacgcttga cgccgccggc gttgagaacc gcaagcaagg gcgctcgaag
     3601 tatggcgcca agcggcctaa ggctgcccag aagtagcggg tgcgggctcg gtaagagccc
     3661 gaaagaagaa gtcaggagag tttgcgaatg ccccgtaaag gttttgttcc acggcgcgaa
     3721 gtgacgaccg actctgtata cagcagcgat ctggtgcaga agtttatcaa ttcgatgatg
     3781 caggacggca agcgctccac ggcgcagcgc attttctacg gcgcgatgga tcagattggt
     3841 cagaagacca atggcgaagc tctagccacg ttcaagaagg ccattgagaa cgtcaagccg
     3901 gtgctcgagg tgaagacgcg gcgcgtaggc ggagcgaact atcaggttcc agtcgaagtg
     3961 aaccctttcc gcaggcagtc gctggccatt cgctggctgc tgctctatgc gcgtacccgg
     4021 tcgggcaaga ccatgaccga caagctggcc gaagagcttc tggacgcggc caatgcccgc
     4081 ggcggcgcga tgaaaaagaa ggaagacgtt catcgcatgg ccgaggccaa taaggccttt
     4141 gcgcactatc gctggtaatt cagccagcgt aggggaggca cgacatgtcg tgcccctata
     4201 actttcagtc ggccggtgag ttttaaaagg caaggtaaca ggggattggg tgggttttca
     4261 gattcaaggg agaaagtcgc ggtaatccag agatggcacg caaagcacaa ctcgcggaaa
     4321 cacgcaacat cggcatcatg gcgcacatcg acgccggcaa gaccacttcg accgagcgca
     4381 tcctgttcta tacgggcgtg acgtacaaga ttggttccgt cgacgaaggc acggccacaa
     4441 tggactggat ggagcaggag cgcgagcgcg gcatcaccat cacttcggcg gcgaccaccg
     4501 cttcctggaa gcgcaatgct aaagagtttc tgattaacat tatcgatacg cctggccacg
     4561 tcgattttac cgtggaagtc gagcgctccc tgcgcgtgct cgacggcgct gtcgcgattt
     4621 ttgacgcggt tgcgggcgta cagccgcaat cggaaaccgt ctggcgtcag gctgacaagt
     4681 accatgttcc gcgattggcg ttcatcaaca agatggaccg tacgggcgcg gatttcgagc
     4741 acgcgctgca aaccatgcgc cagcgtctcg ccgctcatcc tgtaccgatt caatgcccca
     4801 ttggcaagga agacctcttc atcggcgtta tcgattttct cgaagaaaaa gcaatcatct
     4861 ggctggaaga aaccctcggc gcaaaatttg aagtgatgga cgttgcgaaa ctctgggatc
     4921 ccgagtttct ggccggacgt cctgatgttg tcgcggcgct gaagttctgc gcgctggacg
     4981 aagcgtatta caaggaacgc cgcgaaaagt ccgtagaata cattgccgag cacgatgaca
     5041 cgcttgttga gaagtatctg aacggtgaga aaattaccgc cgacgagcta cgcgcttcga
     5101 ttcgccgctc gaccatggca ttgaaactgg ttcccgttct ggcgggttcc gcgttcaaga
     5161 acaagggtat tcagccgctg ctggatgcgg tagtggacta tctgccatcg cctcttgatg
     5221 tgccacccgt ggaaggtacg accgcgaacg atgaagtgga attgcgccgc gcgagcgatg
     5281 acgaaccctt cgccgccctg gcatttaaaa ttatgtccga cccgtttgtc ggccatgtca
     5341 cctttattcg agtttattca ggcgtgttga agtcgggaag ctacgtttat aacgcgacga
     5401 agcaaacgcg cgaacgcatc agtcgccttg tgcgcatgca tgccaacaag cgcgaagaaa
     5461 ttgacgccat ttatgccggc gatatcgccg cctgcgtcgg cctgaaaagc gtaactaccg
     5521 gcgacacgct ttgcgatgaa cagaaaacca tagtcctcga aactatcgaa tttcccgccc
     5581 cggttatttc cgtcgccatc gagccaaaaa ccaaagccga ccaggacaag ttgggcaacg
     5641 ccatgcagcg gctcgcgcag gaagatccga cgttccgcgt gcatagcgac gaagataccg
     5701 ggcagaccat tatttccggc atgggcgagt tgcatcttga aattatcgtc gaccgcatga
     5761 tgcgcgagtt tggcgtgcaa gcgacagttg gacgcccaca ggtggcgtac cgtgaaacga
     5821 ttcgcaagcg cgccgaagcc gagggcaaat acatccgcca gaccggcggc cgcggtcagt
     5881 acggccactg cattatcgaa gttcttccgc tgccttcctc cgcgcacgaa aacatgcacg
     5941 aaatgtcgac cgatgagctg gccatccttg caaaagaaat tagcggagga ggcggcaagc
     6001 cgcgctttga caaggaacac cgcatgttgt ttatcgacaa gacggtgggc ggctcgattc
     6061 ccaaagaatt tatcgctccg atcgatgcgg gaattcgcga agccatggaa aacggcatcc
     6121 tggccggttt tgaaatggtg gacattgcag tgctgctgat cgatggcagc taccacgatg
     6181 tggacagctc ggaaatggcg tttaagattg ccggctccat ggccttcaaa gaagcctgca
     6241 gcaaagccaa gccagtgctg ctcgaaccga tcatgaaggt ggaagtcgtc actcccgaag
     6301 aatatatgcc gcaagtgttt ggcgacttga acgcgcgacg ctgccagatt cagggcacgg
     6361 aaagccgcgg cggcgcgaac gtgattcgcg tggaagttcc gctggcctcg atgttcggat
     6421 acgccacgga cgtgcgcagc cgcacacagg gccgcgccag tttcaccatg cacttctcgc
     6481 attacgcgga agcgccagca gcggtggctg aggaagtgat tgcgaagtcg acaggtaggg
     6541 cgactcgcta agtttttgta gaatttttgc atgagactag atacttgcag ctaaacaaac
     6601 gttcacggtg aggagctgac agagcgatgg cgaaggaaaa atttgagcgc aataagccgc
     6661 acctgaatat tgggacgatc gggcacatcg atcacggcaa aacgacattg acggcagcga
     6721 tcacgaaggt gctgtccaag cacaacccga aggtggcgtt ccgcgcattc gattcgattg
     6781 acaacgcgcc ggaagagcgc gagcgcggaa ttacgattgc cgtgtcgcat gtggagtacg
     6841 agacggcgaa ccggcactac gcgcacatcg attgcccggg gcacgcggac tacgtgaaga
     6901 acatgatcac gggcgcggcg cagatggacg gagcgatcct ggtggtggcg gcgacggacg
     6961 gaccgatgcc gcaaacgcgg gagcacattc tcttggcgcg gcaagtgggc gtgccgtcga
     7021 tcgtggtgtt catgaacaag tgcgactcgg tggaagatcc cgaattgctc gaactggtgg
     7081 aactggaagt acgcgaactg ttgaagttgt atcagtttcc gggagac
//
LOCUS       DASZMB010000149         8409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_5370671, whole genome shotgun sequence.
ACCESSION   DASZMB010000149 DASZMB010000000
VERSION     DASZMB010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8409
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5370671"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1051)
                     /locus_tag="VG272_06780"
     CDS             complement(47..1051)
                     /locus_tag="VG272_06780"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HWF13422.1"
                     /translation="MEPVTHALTSLALSRAGLNKLTRAATPILLVSGVAADVDWVTRL
                     GTAATFLRGHRTATHSIIGTLAIAFTVAVAAWILGKKSRTFTVRLSAALLISAIGATA
                     HLLLDLLNAYGVKLLWPFSEKFYAWDIADSIDSWMLFFLIAGLFVPELFRLVLDEIGS
                     KQKHHGGQRGAIFALVCVGLVIAGRAFLHQRAVALLDSREYRGQTPLLVAAFPKPSDP
                     FLWSGVVETDNAVFNLEVPVGFGRMFDPDQADVHYKPQPVAALKSAVACHTAVKFLNF
                     ARFPLASVQPEGDGFQVRLRDMRFATELPGRRGIIAVVDLNAQSQVIGEHLEYELANN
                     "
     gene            complement(1173..2090)
                     /locus_tag="VG272_06785"
     CDS             complement(1173..2090)
                     /locus_tag="VG272_06785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HWF13423.1"
                     /translation="MTSKVRNAVLIVNPTAGGGRRMSQLEEARRIFRAAGIETDLQLT
                     TAPGEATVLARSAVEESRDLVIVCGGDGTVNEVVNGLACSQVPLAVLPAGTANVLAKE
                     LELPWNLPRAAKRIINAPYKRIALGLAIPEKSSGEPRYFLSLAGAGADGALVSAVRPE
                     IKLKAGILAYWQEGLRQLSKYDFPMFRSSMAGNAINIDASLVVVGRTKHYGGPFKITT
                     EAGLLQPEFELAFVTTRSAWRYIAYLPLLWTGKLRGARHVQFFKTSSLQCASNSESHV
                     QIQVDGEPAGRLPVEFRIVPDALTLAIPD"
     gene            2240..4342
                     /gene="ligA"
                     /locus_tag="VG272_06790"
     CDS             2240..4342
                     /gene="ligA"
                     /locus_tag="VG272_06790"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HWF13424.1"
                     /translation="MAKGANVNKQIEKLREELRRHEYLYFVQDEPEISDIKFDRMMAE
                     LKQLEVEHPELVTPDSPTQRVGGAPRKGFETRRHSPPMMSLDNTYSTEELEDFDRRVR
                     DISGRETISYVTEHKFDGLSISLVYENGVLARGVTRGDGTTGEEVTANVRTIRSIPLR
                     VDPAELKQLHMPLDFEVRGEIVMPLRAFELLNEKQEEEGGKRFSNPRSAAAGAVRVLD
                     PEITRTRRLDFYGYLLLANGRVPTRLHSESLEAMQKLHFKVSPDWRVCPSLEVVKKFI
                     DSWEARRDKLPYEIDGIVIKVNDTGQQQELGFTAKAPRWAVAYKYPARQETTVVREVL
                     FNVGRTGTLTPVAMLEPVQVGGVIVSRSTLHNMDEIARLGLYAGDTVIVERAGEVIPH
                     IIKVVKEGNPRREVVVPEFCPECHSRIHKDPEEVAYRCVNVSCPAKRKQSILHFAGRH
                     AMNIDGLGEKIVDQLVDKAMVKDFADLYDLELEPVAKLDRMAEKSAQNLLDEIAASKK
                     NSLARLIYALGIRFVGERTGQLLAEHFSSLQKLADATAEKLEEVGEVGPKVALSIADF
                     FSEPANRELIQRLKDADLNMTEKRAALEDTRFAGMTFVFTGALARRSRDEGGAEVSRH
                     GGKVTNSVSKLTSYVVVGAEPGSKFEKAKSLGVSVLDEQAFEDLLQGKLKVDGPKGSD
                     RNGMPQNKPKGRKKKIAK"
     gene            4601..5317
                     /locus_tag="VG272_06795"
     CDS             4601..5317
                     /locus_tag="VG272_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HWF13425.1"
                     /translation="MSKLTLSATITLAIGTMFIAGCATKNYVKKSITPIDQKVDQVDQ
                     ASQKRDSTQVADINKTNQTVDEDEKKLSATDEVAKSADGTAKGAMAKSNQNTKDINDL
                     RGVIANIDDYKPAGEATVVHFAVNKDTLTKDEKAKLDAMASQLASQQRYFITVEGFTD
                     QTGTAAINDNLSRARASSVISYLVGSHDVPVYRIHMVGLGSQKLIDEGKGRKGREASR
                     RVEVTLFTAKPLTMGAAGGN"
     gene            5576..6127
                     /gene="rnpB"
                     /locus_tag="VG272_06800"
     ncRNA           5576..6127
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VG272_06800"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(6224..7564)
                     /locus_tag="VG272_06805"
     CDS             complement(6224..7564)
                     /locus_tag="VG272_06805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWF13426.1"
                     /translation="MPDLILRKGVSHLMRALPKTDEVSSLLRSPTLAVVTAYALRMFL
                     MWLSHQKAGEHLLPVTGLEAGMLALSLASGKGFFGPFPGYAAPTAWLAPGYPAIWALA
                     IRLTHIYSGAIVLLAQTINCAFSAMTCWPIFSIGKKLFGEKVGLASCWLWVFLPFSIL
                     MSLEWTWDQCVTAFLLALIVDFTFRVRDSMSALRWAGYGLLGAAAALVNPAASALLPF
                     LFAWLIWARWKVSMKSPMLYAQAALMFFLAVLPWSIRNYHALHGWVFVKSNFGDALYV
                     GNHPASFAVDPQPMINSEERARLISEGEPQFNKNEEHLAVRYIESHPGEFVKKTGGRV
                     IDTWTAVDNSFTDRWVSSLHLVGAEIFFCVVFSLLSFAGLVLALRANSIDPIPLAICL
                     ILFPAPYYISLAEMRYRHPLDPILTVLTAYVVSRIWQHVSERTAVENLQTSAAA"
     gene            complement(8209..>8409)
                     /locus_tag="VG272_06810"
     CDS             complement(8209..>8409)
                     /locus_tag="VG272_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13427.1"
                     /translation="WSFHTGGRIASAAVSYSVNGRQFIAVGSGGGSETEGRLAKLYPE
                     SAPHIAQPASTLFVFALPEKEK"
BASE COUNT         2050 a         2221 c         2432 g         1706 t
ORIGIN      
        1 cgaaccccgc acaggctgaa gcctatgcca caacaggctc cgcaatttaa ttgtttgcaa
       61 gctcatattc caaatgctcg ccaatcacct ggctttgtgc atttaagtcc accacggcga
      121 taattccgcg gcgtccgggg agttccgtgg caaagcgcat gtcgcgcagg cgcacctgaa
      181 acccatcgcc ttcgggctga acactcgcca acggaaatcg cgcaaaattc aaaaacttca
      241 cggccgtgtg gcaggcgacc gcgcttttca gagcggcaac tggttgcggt ttatagtgca
      301 cgtctgcttg atccggatcg aacatccgcc cgaatcccac cggaacctcc aggttaaaga
      361 ccgcgttatc cgtctccaca actcccgacc ataaaaatgg atccgacggc ttcggaaacg
      421 ccgcaaccag aagcggagtt tgcccccggt attcgcgcga atcgagcagc gcgacggcac
      481 gctggtgtaa gaacgcgcgc ccggcaatca ctaagcccac gcacactaga gcgaatatcg
      541 cgccgcgctg gcctccgtgg tgcttttgct ttgagccaat ttcatcgagc accaagcgaa
      601 acaattcggg aacgaaaagc ccagcgatca gaaagaaaag catccacgag tcaatcgagt
      661 cagcgatgtc ccacgcgtaa aatttctcgc tgaaaggcca gagcagtttc acgccgtatg
      721 cgttcagcaa atccagcaac aaatgcgcgg tcgcgccgat cgcggaaatc aacaatgccg
      781 cggagagcct cactgtaaat gttcgagact ttttgcccag tatccaggcc gcgaccgcga
      841 ctgtgaaggc aatcgccaac gttccaataa tggaatgggt cgctgtccga tgcccgcgca
      901 ggaatgttgc cgctgtgccg agccgagtga cccaatccac atcggcagcg acgccggaga
      961 cgaggagaat cggcgttgct gcgcgcgtga gtttattgag gcctgcgcgg ctcaaggcga
     1021 gggacgttaa cgcgtgtgtg acaggttcca tttcgcctag tttttcttcg ccctcggtag
     1081 gacaggcttc agcctgtctg cttttgatct tttttccaat ttacagaagg cgaaagaaga
     1141 caggctgaag cctgtcctac tgacggcttc acctaatccg ggatggctag ggtgagagca
     1201 tcgggcacga tgcggaactc gacggggagg cggccggcag gctcgccatc aacttggatc
     1261 tggacgtgcg actcggagtt cgaagcgcat tgcagcgagc tggttttgaa aaattgaacg
     1321 tggcgtgcgc cgcgcaattt tccagtccac agcagaggca gataggcgat atatcgccaa
     1381 gcgctgcgag tagttacgaa ggcgagttcg aactccggct gcaacaggcc tgcttcggtc
     1441 gtgatcttga acgggccgcc gtaatgtttc gtgcgcccga cgaccacgag cgaggcgtcg
     1501 atattgatcg cgttgcctgc catggaactt cgaaacatgg ggaagtcgta tttggaaagt
     1561 tgccgcaagc cctcttgcca gtacgccaga ataccggcct tcaacttaat ttcggggcgc
     1621 accgcggaca cgagcgcgcc atcggcgcct gcgccagcca aactcagaaa ataacgtggt
     1681 tcgccgcttg atttttcagg gatcgctagt cccagcgcta tgcgcttgta gggcgcattt
     1741 atgatgcgct tggcggcgcg cggcaaattc cagggaagct cgagttcctt ggccagcaca
     1801 ttcgccgtgc cggccggcaa aacggccagt gggacctggc tgcaagccag tccatttaca
     1861 acttcgttca ccgtgccgtc gcctccgcaa acgatcacaa ggtcccgcga ctcttcgacg
     1921 gcgctgcggg caaggaccgt ggcctcgcca ggggcagttg tcagttgcaa atcggtttcg
     1981 attccggccg cgcgaaaaat gcgccgcgct tcttcgagct gactcatccg gcgaccgccg
     2041 ccggccgtgg ggttcacgat taagaccgca ttacggactt tggatgtcaa ttgaatggct
     2101 ccaatcgctc ccaccagaat ttttctagtc tgacaactta ataagataac gcctttggtc
     2161 cgggaaaggt acaggctgtt ttggcgctgg ggctgcattg tatactgaag ggctgattct
     2221 gtcggctttc tgagggtgca tggccaaggg cgcgaacgta aataaacaaa tcgaaaagct
     2281 gcgcgaggaa ttgcggcggc acgagtattt gtacttcgtg caggacgagc cggaaatctc
     2341 ggacatcaag tttgaccgca tgatggccga gctcaagcag cttgaagtgg agcatcccga
     2401 gctggtcacg ccagattctc cgacgcagcg cgtgggcggc gctccgcgaa aagggtttga
     2461 gacgcgcagg cactctccgc ccatgatgag cctggacaac acgtactcga ccgaggaact
     2521 cgaggatttc gaccggcgtg tgcgtgatat ttccggacgc gaaacgatta gctatgtcac
     2581 cgaacacaaa ttcgacggcc tgagcatttc gctggtctat gaaaatggcg tgctggcgcg
     2641 cggcgtcacg cgcggcgacg ggacgaccgg agaagaagtg accgccaatg tgcgtacgat
     2701 tcgttcaatt cccttgcgag tcgatcccgc ggagctcaaa cagctgcaca tgccgctgga
     2761 ttttgaggtg cgcggcgaga tcgtgatgcc gcttcgcgcg tttgaactgc taaacgaaaa
     2821 gcaggaagag gagggtggca agcgtttttc caatccgcgc agcgctgcgg ctggcgccgt
     2881 acgcgttctc gatccggaga ttacacgcac aagacgcctc gatttctatg gttatctttt
     2941 gctggcgaac ggcagggttc ccacgcgcct gcattccgaa tcgctggaag ccatgcagaa
     3001 gctacatttt aaggtctcgc cggactggcg cgtgtgcccg tcgctggaag tggtaaagaa
     3061 atttatcgac agctgggaag ctcgccgtga caaattgccg tacgaaatcg acggcatcgt
     3121 aatcaaggtg aacgatacgg ggcagcagca ggaactcggc ttcacggcca aggcgccgcg
     3181 ctgggcggtc gcctataaat atccggcgcg gcaggaaacc acggtagtgc gagaagtatt
     3241 gtttaacgtg gggcgtacgg ggacattgac gccggtggcg atgcttgagc cagtacaggt
     3301 gggcggagtg atcgtctcgc gctccacgct gcacaacatg gacgaaattg cgcggcttgg
     3361 gttgtatgcg ggtgacacgg tgattgtgga gcgcgcgggc gaagtgattc cgcacatcat
     3421 caaggtagtg aaggaaggca atccgcgccg cgaagtggtg gttccggaat tttgcccgga
     3481 atgccacagc cgcatccaca aggatcccga ggaggtcgcc taccgctgcg tgaatgtcag
     3541 ctgcccggcg aagcgaaagc aatccattct gcacttcgcg ggacgccatg cgatgaatat
     3601 cgacgggctc ggcgaaaaaa ttgttgacca gcttgtggac aaagcgatgg tcaaggattt
     3661 cgcggacctc tatgacttgg aactggagcc cgtagcgaag ctcgatcgaa tggccgaaaa
     3721 gtccgcgcaa aatctactgg acgaaattgc agccagcaag aaaaacagtc tcgcccggct
     3781 aatctatgcc ctcgggatac gcttcgttgg agagcgcacg gggcagttgc tggcggaaca
     3841 tttttcttcg ctgcaaaaat tggctgacgc gacagcggaa aagcttgagg aagttggtga
     3901 agtcggtcca aaggttgctc tgagcatcgc cgattttttc tcagaacctg ccaaccggga
     3961 actcattcag cgcttaaaag atgcggatct gaacatgacg gaaaaacgcg cagctctcga
     4021 ggatacgcgt ttcgccggaa tgacattcgt ttttactgga gcattagccc gccgttcgcg
     4081 cgacgaaggc ggcgcagagg tttctcgcca cggcggaaag gttacgaact cggtcagcaa
     4141 attgacgagt tacgtggttg ttggcgcgga accgggatcg aaatttgaga aggcgaagtc
     4201 gcttggcgtc tccgttttag acgagcaagc gtttgaagat ttattacagg gaaagctaaa
     4261 agtggacggt cccaagggtt cggaccggaa tgggatgccc caaaataagc cgaaagggcg
     4321 taagaaaaag attgccaagt gataggcaga taaggggaaa ggtaccccgg aatggcataa
     4381 accgtgtaaa gattacctgc tataagggac agcagcactt gcttcgccac cagccgacct
     4441 ctccgtattt tcttgtatga atgggactta agagtacagt gaaatttatt cccaaattgg
     4501 ttgactacgt gcgatagtaa acttgagtct tgggagaacg gagaattccg gctatggctc
     4561 aaggggccca aaactgagat tcgtaagggg agaatgcaca atgagtaagt taactttgtc
     4621 ggcaacaatt acacttgcaa tcggtaccat gttcatcgcg ggttgcgcga ccaagaatta
     4681 cgtgaagaag agcattactc caattgatca aaaagttgat caggtggatc aggcttcaca
     4741 gaaacgggat tccacacagg tcgcagacat caataagacg aatcagactg ttgacgaaga
     4801 tgagaagaag ctgtccgcca cggacgaagt tgcgaagtcg gcagacggta cggccaaagg
     4861 cgcaatggcc aagtccaacc agaacacaaa agatattaat gacctccgcg gcgtgatcgc
     4921 gaacatcgac gattacaaac ccgcaggcga ggccaccgtt gtgcattttg cagtgaacaa
     4981 ggacactttg accaaggatg aaaaggcgaa gctggacgct atggcttcgc agttggcgtc
     5041 tcagcagcgc tatttcatca cggttgaagg gttcacggac caaactggaa ctgccgcgat
     5101 caacgataat ctgagccggg ctcgtgccag cagcgtgatc tcgtatctgg ttggaagcca
     5161 tgacgttccg gtttaccgca ttcacatggt cggcctgggt tcacagaaac tgatcgacga
     5221 gggaaagggc cgcaagggac gtgaggccag ccgccgcgtc gaggtgactc tgtttaccgc
     5281 caagccgcta acgatgggcg cagccggggg caactagctc gtttcgcaag tttaccttct
     5341 ttgagagtgt gggtgccggc cacgggctgg cacccacacc tagagaccaa gttgtgcccg
     5401 cacttaaatc cctttttttg caacctgtgt tcttcccttc aatttctaaa ctcctaatat
     5461 gggtgtgagc ccggtcgcag gttgggactt cttcgggcct aaacgtttat tttgcgggat
     5521 gccttttcac catgcctgcc cggcgctcct tataaaaatg atatacttgc cttggcgggc
     5581 aagccgggtg atcgctgggc caaacttcgc aagaaggaag gccgggagga aagtccggac
     5641 accgtggtct tcgtgcagtt agcggtaacg ctcactgtgc cgagactaaa gggcgacgcg
     5701 cctggtaacg ccagggggct cgtttttcgc gccgaacgcg caagcgattt gatgcgggaa
     5761 accggctacg gacagtgcca cagaaaataa accgccggtg catttgcttc tcggtggcta
     5821 tccgaaggaa aacctttgga cgacctgaaa gcaactcgcg caagcgagct gcagaaagcg
     5881 attgtggctg gtaagggtga aacggtgggg taagagccca ccgcctcgat ggcaacatcg
     5941 agggcacggc aaaccccgcg tggtgcaagg ccaaatagga ggggagagcc agcccggctc
     6001 gtcaggaccc tcgggtaggc tgctggagct caggtgcgaa cctgagccta gaggaatgat
     6061 cgccacagcg cagaggctgc aaagtctacg cgctggacaa aatccggctt acaggcttgc
     6121 tcgccatttt ggtttctcgt attgtgtggg aattttcgcc tgcctgccgc ggctttcaga
     6181 cttcttgatt cgcaccatgt gacgtgcaat gacgactaaa ccgtcaggcg gcggcgctgg
     6241 tttgtaaatt ttcgacggcc gtgcgttccg acacgtgctg ccaaattcgc gaaaccacat
     6301 aagcggtcaa gacggtaagg atcggatcga ggggatgcct gtagcgcatt tcggcgagcg
     6361 aaatgtaata aggcgcgggg aataagataa ggcagatggc cagaggaatc gggtcgatcg
     6421 aatttgcgcg caaggctaaa actagacccg cgaacgagag cagggaaaag acgacgcaaa
     6481 agaaaatttc cgcgccgaca aggtgaagcg atgagaccca ccggtcagtg aaggaattgt
     6541 ccaccgccgt ccacgtatcg atgacgcggc ccccggtttt tttgacaaat tcgccggggt
     6601 gcgattcgat gtatcgcacg gccaaatgtt cctcgttctt gttgaattga ggctcgcctt
     6661 cagagatcag ccgggcgcgc tcttcggaat tgatcatggg ttgtggatcg acggcaaaag
     6721 aagccgggtg atttcccacg tacaatgcat ctccaaaatt cgatttgacg aagacccacc
     6781 catgcagggc gtggtaatta cgaatcgacc agggcaggac agccagaaaa aacatcagtg
     6841 ccgcctgcgc atagagcatg ggcgatttca tgctaacttt ccaccgagcc cagattaacc
     6901 atgcgaaaag gaatggcaaa agagcggacg ccgcaggatt caccagagcc gctgccgctc
     6961 caaggagtcc gtaacctgcc cagcgcagcg ccgacatcga gtcgcgaacc cgaaacgtga
     7021 agtctacaat tagcgcaagc aggaacgcgg ttacgcactg gtcccaggtc cattccagcg
     7081 acattagaat tgaaaacgga aggaataccc acaaccaaca agaagccaag ccgacctttt
     7141 ctccgaacag tttttttccg atggaaaaaa tcggccagca cgtcattgcc gaaaacgcgc
     7201 aattgatggt ttgggcgagc aggacgattg ctccggaata gatgtgcgtc aatcgaatgg
     7261 cgagcgccca gatcgcaggg tatccgggag cgagccacgc ggtgggggca gcgtatcccg
     7321 gaaacggccc gaaaaatcct ttgcctgatg caagcgacaa cgccagcatt cccgcctcga
     7381 ggccggtaac cggcagcaga tgctcgcccg ctttttgatg cgagagccac atcaaaaaca
     7441 tgcgtagcgc atacgccgtc acgacggcta gcgtcggaga gcgaagcagt gacgaaacct
     7501 cgtccgtttt cggtaaggca cgcatcaggt gcgaaacacc ctttcgcaaa attagatcag
     7561 gcatttagcg tacctttgcg gcgaccgcat ttccactggc tcgcgagatg ggtttgcctc
     7621 ttcatcaagg ttagcaaatg gacacatccg cgaaggcgcg tcccgccggg ccggaaccaa
     7681 tcggcaaact ttcacatcca gaaatttagc ctcggcaaga aaatgctggt tggttctaat
     7741 tgtggtataa attcatcgag caatacgaat cctaaaaggc aaggaacatg ctagaacgtt
     7801 tcatccccgt ccgacagtgc ccccgctgtg gcagttttaa tgtccatcgt cacaggggtg
     7861 ggagccaaat taaacgcatt gtcatcaggc tggtcctgat acgcaaatac tcctgcatgt
     7921 attgcaacag cctttactac ggcttcctgt ttagcaaaag gctgcctgaa aaagagcggg
     7981 caagcaggga agcgaagctt atgacgcgca cgtccaagtc ctgaaataca aaaatttctc
     8041 atacgctgcc gcgaagtaac agcttggatt tataaccaga gcagagattc aggctggaaa
     8101 ctacctgaag ctcgatactt gcgttcatct cgctgggttt tccgttttga aaagtatcgt
     8161 aactgttggc ttggcattct gctcaaaaag tttcgggatg cgtaaaaatt atttttcctt
     8221 ttcgggcaag gcaaacacaa acagtgtcga cgcaggttgc gcgatgtgcg gcgcggattc
     8281 gggatacaac ttggccaggc ggccttcggt ctccgaaccg ccgccggacc ctaccgcgat
     8341 aaactgccgg ccgttgaccg aatagctgac cgcggcggaa gcgattcgtc cgcctgtatg
     8401 gaatgacca
//
LOCUS       DASZMB010000150         7084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_139907, whole genome shotgun sequence.
ACCESSION   DASZMB010000150 DASZMB010000000
VERSION     DASZMB010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139907"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..236)
                     /locus_tag="VG272_06815"
     CDS             complement(42..236)
                     /locus_tag="VG272_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13428.1"
                     /translation="MTARLIERRIRWSGRLIAAGLIVQMLTLLATHPLAFICFALVGC
                     PLVGAGILIFLYSLVEHEAV"
     gene            complement(233..874)
                     /locus_tag="VG272_06820"
     CDS             complement(233..874)
                     /locus_tag="VG272_06820"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HWF13429.1"
                     /translation="MGGTTTILIVFTAMVALLGLLTSMRPEFTAARGGKIMAFVALCI
                     LPVVSIWAGFHEQVERSTSTQFCLSCHVMDKFGKSLTVDDKSFIPAAHFQNNFVPRDH
                     ACFTCHTDYTMFGDYKAKWRGVHHVMVQYFGTIPKPEDIKLYTPYNNRECLHCHAGAR
                     SYEEASSHHKTPDMLAKAASNELSCTSSGCHDIVHNVGDLGDATFWTPKAEPK"
     gene            complement(1155..1514)
                     /locus_tag="VG272_06825"
     CDS             complement(1155..1514)
                     /locus_tag="VG272_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWF13430.1"
                     /translation="MTRTMGYSKLLIDTSLACIFAMSLGSALRASPAPQKSSQDVYLD
                     KCSVCHAKDGSGNTAKGRKVKAKDLRSAEIQKLTDAELIKDVGQGKGKDMDGFEKELG
                     ADMVKQLVVYCRQLAKP"
     gene            complement(1565..3586)
                     /locus_tag="VG272_06830"
     CDS             complement(1565..3586)
                     /locus_tag="VG272_06830"
                     /inference="COORDINATES: protein motif:HMM:NF023282.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MtrB/PioB family outer membrane beta-barrel
                     protein"
                     /protein_id="HWF13431.1"
                     /translation="MNPFSRSALLAVALLVITPFAYAQDGTEPVPMAVGDFKLSGSAT
                     AGYRFTDVKGYVPQYQEMFDLRKGLRLLDFNLYGDSEGTKNAFADDFSVQLSGLGGDP
                     FPSAQFTISKKKVYDFRVDWRQSYYFWNQNDNVVLPIAAVTTGISKGLTSNQDWATVR
                     KFGSADLTLYATNNLRFHFDYYRPSDQGTTFTTRAPDFFGSPGFWGTYVRANPYVLNA
                     PLTDNTNRITGGLDYSLKSWNFHYSVGYQTFTENIGFNNVTTPELSINPIASSVAEPL
                     ANFSMSQFRRLTTPISEFSFIGKPLPKLEWRGGYIFYRYAGPVTFNQAFNGIAPSSGG
                     PLAPYAISESANAKVTEPNHIINQGLTYHLFSWWSLDADYRYTRFTSDSTGNFQSLFN
                     ATATAGSTNVIWRDGLSELAFSMDFTPIRSLIIRPGVQFMKSDVESLTNGVAVPSLTL
                     RTNSVRPEISFGYEPSKIFSVRGDFHSTDNGSSYTAITPSSQVATHFVVRVHPLEKLT
                     IEDEISVANNKLLVSNFQNNIRSNAITVSYSLGERFSIFGGFSYDSYIAQGNILYARG
                     TAPLADTLRDQELNRVWSGGIEAKPTKRIGLRLSGNFDRSSGVGAISGEPPAYGPSVF
                     PLVTGTAFYNFPVVGQLGVDLQRTYYEEQIVTVNNFSANLLTIRWTKSF"
     gene            complement(3597..4619)
                     /locus_tag="VG272_06835"
     CDS             complement(3597..4619)
                     /locus_tag="VG272_06835"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HWF13432.1"
                     /translation="MALATCLASVTFAQKRPESVQSPSVASRAPEFSAPAAEFSGPEA
                     CKDCHKNETIQYEKTGHSKIVFPGKDYIHGCETCHGPGKAHGDAVQAAHGDDAEVAKA
                     LVEHPIFAFKGKAEENAARCLTCHTSSKQQELFQHSEHAGHGVSCGECHSAHLVDEVK
                     DLSKGDLSYPQSYFFNLPKLADENRWRHNSLLKLSEPALCFTCHQTVQAQFAQPEHHR
                     VPEGLMKCSDRHNPHGSGNVASLTSSGSETCVKCHVEKRGPFVFEHPAVKIEGCVTCH
                     NPHGSPNRMLLVRREGRQLCLQCHTGFHGQPQVPHSRLGFQTSGECIRCHVTIHGSSL
                     DPNFLR"
     gene            5710..>7084
                     /locus_tag="VG272_06840"
     CDS             5710..>7084
                     /locus_tag="VG272_06840"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWF13433.1"
                     /translation="MKKPSRRELLKLGGATAFTALGASVLDGCGSIASNIPSVTSGCT
                     QLSDIQHVVIFIQENRSFDHYFGSFHGARGFSDQSLAFNQPYPGNMNAAPVGKLLPYH
                     LDTNTINAACTHDITHDWVSQHQSWDNGAMDGFVTSRVAANANDAILTMGYYTRTDLP
                     FYYAAADAFTLCDNYFCSVMGPTDPNRLYTMAASIDPDGRNGGPLIETLVGNRSSFYG
                     RLTYTTMPEQLQARGISWKVYSSPDENLLGGILSDNVLPYFKNFQDPTSALYKSAFGP
                     QFPTDFLADIVSNNLPQVSWLIGSTVASDHPPAPSVFGESELALITSALMANPAVWAK
                     TVLFLTFDENGGFFDHVPPVTAPPGTPGEYITAPTVPDVSAIGVPPTSGPIGLGFRVP
                     MLVISPYTRGGFVASDVFDHTSILRFLETRFGAEVPNLSAWRRATVGDLTSAFNFKSP
                     DTSVPNLP"
BASE COUNT         1579 a         1918 c         1922 g         1665 t
ORIGIN      
        1 gctttcttaa tgtctaactc cactgcgggc gaggaactcc atcacacagc ctcatgttct
       61 acgagcgaat agagaaaaat caaaattccg gcgccgacga gagggcatcc taccagcgca
      121 aagcaaataa acgccagcgg atgcgttgcg agcagcgtca gcatctgcac aatcaatccc
      181 gcggctatca gccgaccgct ccagcgaatg cgccgttcga tcagtcgcgc ggtcatttcg
      241 gctccgcctt cggcgtccag aacgtggcat cgccgaggtc accaacgttg tgcacaatgt
      301 cgtggcagcc gctggacgtg caggagagct cattgctggc cgccttggcc agcatatccg
      361 gggttttgtg gtgcgaagag gcttcttcgt aagagcgcgc accggcgtga cagtgcaggc
      421 actcgcgatt gttgtatggc gtgtagagct ttatgtcttc cggcttggga attgttccga
      481 agtattgaac catcacatgg tgcacaccgc gccacttcgc cttgtagtct ccgaacatgg
      541 tgtagtccgt gtgacaggtg aagcaggcat ggtcacgcgg cacgaaatta ttttgaaagt
      601 gagcggcagg aataaaactt ttgtcatcca ccgtcaggct ttttccaaat ttgtccatca
      661 cgtggcagga gaggcagaac tgcgtggaag tggagcgttc cacctgttca tggaagcctg
      721 cccagatcga gaccacaggc aaaatgcaca gcgccacgaa ggccataatt ttcccgccgc
      781 gcgctgcggt aaattccggc cgcatagatg tgagaagccc aagcagcgcc accatggccg
      841 tgaagacaat cagaatggtc gttgttccgc ccatcaaatt aaatcagctc cttagatcga
      901 tctcttgaag caaactcgtg cggcgactcg gttcagggaa attgttgccg gtcgcgtgtt
      961 acgcgcgggt taacgggccg cagcaagagt gtccttattc tgtcattccg agcgaagtga
     1021 ggaatctttc ttcgatttac gtgcaaagaa ataaaagaag agagattcct cgcttcgctc
     1081 ggaatgacag agctttaatt ttccgccggc ggttaagtcg ccgggcactc cactgaaaaa
     1141 acattgcggg actattaagg cttagctaat tgccggcagt acacaaccag ttgtttcacc
     1201 atgtccgcgc ctaattcctt ttcgaatccg tccatgtctt tgcccttgcc ctggccgaca
     1261 tctttaatta attcggcgtc agtcaacttc tgaatctccg cggaacgcaa atccttggcc
     1321 ttcaccttgc gcccttttgc tgtgttgccc gagccatcct tggcatggca gacggaacat
     1381 ttgtccagat agacgtcctg agagcttttt tgaggcgcag gagaagctcg caacgcgctt
     1441 ccaaggctca ttgcaaaaat gcaggcgagg ctcgtgtcga tcaaaagttt ggaatacccc
     1501 attgtcctcg tcattcgttc tccttcgatt cgcagaatgt ttattccgtg agaactatac
     1561 aaacttagaa tgacttggtc catcggatag tcagcaaatt agcgctgaaa ttattcaccg
     1621 tcacaatttg ttcctcgtaa taggtgcgct gcaggtccac gccaagctgg cccacgaccg
     1681 ggaaattgta gaaggccgtg ccggtaacga gcggaaacac cgatggcccg taagcgggag
     1741 gttctccact gattgcgccg acgccgcttg accggtcgaa atttcctgaa agccgcaagc
     1801 caatccgctt ggtcggcttc gcttcgatgc cgccggacca cacgcggttc agctcctggt
     1861 cgcgcaaagt atcggccagc ggcgctgtgc cgcgcgcgta gagaatattt ccctgtgcga
     1921 tgtaactgtc ataactgaat ccgccgaaga tggagaatcg ctcgcccaga gagtaggaaa
     1981 ccgtgatggc gttcgagcgg atattgtttt gaaagttgga gacgagcagc ttgttgttgg
     2041 cgacgctgat ttcgtcctcg atcgtaagct tttcgagcgg atgtacgcgc acgacgaagt
     2101 gcgtggccac ctgcgagctg ggtgttatgg ccgtatacga ggaaccgttg tccgtgctat
     2161 ggaaatcgcc ccgcacgctg aaaatttttg aaggctcgta gccaaaactg atttccggac
     2221 gcaccgaatt cgtgcgcagc gtcagcgagg gaacggccac cccgttggtc aaggattcga
     2281 cgtcagactt catgaactgc acgccaggcc gaataatcag ggaacgtatg ggggtgaagt
     2341 ccatgctgaa agcgagctcg ctcagcccgt cacgccaaat cacgttggtc gatcctgcgg
     2401 tggccgtggc gttgaacaag ctctgaaagt tccctgtgga gtcgctcgtg aagcgcgtgt
     2461 agcgatagtc agcgtccagg ctccaccacg aaaacagatg ataggtcagg ccctggttga
     2521 tgatgtgatt cggctccgtg acctttgcat tcgccgactc gctgatcgcg tacggagcaa
     2581 gtggaccgcc gctgctgggc gcaattccgt tgaatgcctg gttgaatgtg accggcccgg
     2641 cgtagcgata aaaaatgtat ccgccgcgcc attccagttt gggcaacggc ttcccgatga
     2701 acgaaaattc actgatcggc gtggtcaacc ggcggaattg cgacatggaa aaattcgcga
     2761 gcggctccgc gacggagctg gcgatcgggt tgatactgag ctccggcgta gtcacgttat
     2821 tgaaaccgat attttccgtg aaggtctggt atccgacgga ataatggaaa ttccagcttt
     2881 tcaagctgta atcgaggccg ccggtgatgc ggttggtgtt atccgtaagc ggcgcgttga
     2941 gaacgtaagg attggcgcgt acgtaagttc cccaaaaccc tggtgagcca aaaaaatccg
     3001 gcgctctcgt ggtgaacgtt gtcccttggt cagagggacg gtaataatcg aaatggaagc
     3061 gcaggttatt cgtggcgtaa agggtaaggt ccgcggagcc gaacttgcgc accgtcgccc
     3121 aatcctgatt ggaagtcagg cctttggaaa tccccgtggt caccgccgcg atgggcaaaa
     3181 ccacattgtc gttctggttc cagaagtagt agctttgccg ccagtcgacg cgaaaatcgt
     3241 aaactttctt tttggagatc gtgaactgtg ccgacggaaa cggatcgccg cccagcccgc
     3301 tcagttgcac ggaaaaatcg tccgcgaagg catttttggt gccttcggaa tccccgtaaa
     3361 gattgaaatc gaggagccgg agaccttttc gcagatcgaa catctcctga tactgcggga
     3421 cgtatccctt tacgtcggtg aagcggtatc ccgccgtggc cgacccggac aacttgaagt
     3481 cgccgacggc catgggaacg ggttcggtgc catcctgagc gtaggcgaaa ggcgtaatca
     3541 ccagcaaagc gactgctagc agtgcggatc gagagaacgg attcatgaag tcaccctcat
     3601 cgcaagaaat tcggatccag gctcgatccg tgaatggtca catggcagcg aatgcattcg
     3661 ccgctggtct gaaatcccag ccgactgtgc ggaacttgcg gttgaccgtg gaacccggtg
     3721 tgacattgca ggcaaagctg ccggccctcg cggcgaacta acagcatgcg attgggactt
     3781 ccgtgcggat tgtggcaggt cacgcagccc tcgattttta ccgcgggatg ttcgaaaaca
     3841 aacgggccgc gcttctccac atggcactta acacaggttt ccgatccgga actggtcagg
     3901 cttgctacgt ttcccgagcc atgcgggttg tggcggtccg aacacttcat caaaccttcc
     3961 ggaacgcggt gatgttctgg ctgcgcaaat tgcgcttgga ccgtctgatg gcaggtgaag
     4021 cagagagcag gctcggataa tttaagaagg ctgttatgcc gccagcggtt ttcgtccgcc
     4081 agcttgggca ggttgaaaaa ataactttgc ggatagctca gatcaccctt gctcaagtcc
     4141 tttacttcgt cgaccaggtg cgcggagtgg cactcgccgc aggaaactcc gtgcccggcg
     4201 tgttcagaat gctgaaatag ttcctgctgc ttgctgctgg tgtggcaggt gaggcaacgc
     4261 gcggcgtttt cttccgcttt gcctttaaag gcaaaaatgg gatgttcgac aagtgctttg
     4321 gcaacttccg cgtcgtctcc atgcgcagcc tgcaccgcat cgccatgcgc ttttccggga
     4381 ccgtggcacg tctcgcagcc gtgaatgtaa tccttgcccg ggaaaacgat ctttgagtga
     4441 cccgtcttct catattgaat ggtttcgttc ttgtgacaat ctttgcaggc ctcggggcct
     4501 gaaaattccg cggcgggagc ggagaattcc ggagcgcgac tcgccacgga tgggctttgc
     4561 acggattcag gccgcttttg cgcaaaggtt acactcgcga ggcaagtggc cagagccatt
     4621 gccaggagtg acgctcgaag aaaaataggt gttcgattta tctttcgcct agacggcatg
     4681 ggtgtcattg gtcgaccgcc aatctttctg aaaaggatta gaaaatcgta atccgtgcac
     4741 ataccgctta aaggaaacat aatttttgaa gtgcgtgagc ccgctccacg cggcctcacc
     4801 agacccaaca gcaattccgg acaatcaatg ggatcgccgg acatgttgtt gtaacctagc
     4861 cactgcgctt accgtaattc ggcaggctca tcattattgg tgtgcggaaa ccttatctgt
     4921 gacccatgtc aaaaccccgc ttgcgatccg gacgcaaagt tgtgaatcac cttcatatac
     4981 gcggaaaata acgagcacga tatgccacgc gataaataat aaagtttaag cagaccacag
     5041 ttccttgccc gaacgacgcg atcttcgctg tcggttccat tctgcaatta cctcggcagt
     5101 aacacgcgca ggcttgagtt aaattgtcaa taataatgac aggctactct agcatcaagg
     5161 ctacatgttt gcttcattca tattttctat gtgtaacgcc tgcgatacgg catagcaatg
     5221 tcacatgcac gagacatgcc gttcttcgga tcttgtccga atccgtttcc ccgcccttgg
     5281 cgggtgcctc agtttcagtt ctgcgcaagg atatttggtc ccgattcgcg gcggcttcag
     5341 tcctttatgg atcgtggaaa acgtcgccat cctaatttgt gaggtcgttg ttcaagcgcg
     5401 cacttaattt cccgaattgg ggaaaaatgc attttttgat tcccggttcg gcaaaccgga
     5461 ttgaagcaac ggttcagaaa ctacgaggcg tgcccatagg ttgaccggag taagccaccg
     5521 ccagtcacct ggtcattgaa tatgcaattt ctccggcggc gtaacgatgg caccctcaag
     5581 gtgtcgccgg agtggatgaa ttgtcccacg ctcagcgggt atacagtatc cccccgcgat
     5641 ttctaggatg gctcgtgcat tagaatagac accccagcga gcggttgcgg aaagggagtc
     5701 atggcatcct tgaagaagcc ttctcgcaga gaactcttga agttaggagg cgctaccgca
     5761 tttacagctc ttggcgcctc ggtgttggat ggttgcggca gcattgcatc caatattcct
     5821 tccgttactt ccggctgcac gcaactgtcc gacattcaac acgtcgtaat ttttatccag
     5881 gaaaatcgtt ctttcgatca ttatttcggc agcttccacg gcgcgcgcgg attttccgac
     5941 cagagcctgg ccttcaatca gccgtatccc ggaaatatga acgcggctcc tgtcgggaaa
     6001 ttattgccgt accacctgga tacgaacacc atcaacgcgg cctgcacaca cgacatcacc
     6061 catgactggg tatctcaaca tcagagttgg gataacggcg cgatggacgg cttcgttacg
     6121 tcgcgcgtgg ccgccaatgc caacgacgcg atcctgacga tgggttatta cacgcggacg
     6181 gaccttccct tttattacgc cgcagccgac gcgttcacgc tttgtgacaa ctatttttgt
     6241 tcggtgatgg gcccaacgga tccgaatcgc ctttacacca tggccgcctc gatcgatccg
     6301 gacggaagaa atggcggccc gctgattgaa actctggttg gaaatcgctc ctcgttttat
     6361 ggccgcctta cgtatacgac catgccggaa caactgcaag cacgcgggat ctcctggaag
     6421 gtgtattcct cgccagatga aaatttgcta ggcggcattc tctccgacaa tgttttgccc
     6481 tatttcaaaa atttccagga tcccacatcc gcgctgtata aaagcgcctt tggaccgcaa
     6541 tttccgacag attttttggc ggacattgtt tccaacaatt tgcctcaggt ttcgtggttg
     6601 atcggttcca cggtggcttc ggatcatccg cccgctccgt cggtgttcgg cgaaagtgaa
     6661 ttggcactca tcacttccgc gctgatggcg aatccggcag tctgggccaa aaccgtgctc
     6721 ttcctgactt ttgacgaaaa cggcggattt tttgatcacg tgccgccagt cacagcgccg
     6781 cccggaacgc ccggcgaata catcaccgct cctaccgttc cggatgtaag cgccatcgga
     6841 gtgccgccga cttccggtcc cattggcttg ggatttcgcg ttcccatgtt ggtcatctcg
     6901 ccctacactc ggggcggatt cgttgcttcg gatgttttcg atcacacatc tatattgaga
     6961 ttcttggaga cgaggttcgg cgcggaagtt cccaacctga gtgcgtggcg ccgcgcaaca
     7021 gtcggcgatc tcaccagcgc attcaatttc aaatccccgg acacttccgt tcccaatctt
     7081 ccga
//
LOCUS       DASZMB010000151         5718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_5372736, whole genome shotgun sequence.
ACCESSION   DASZMB010000151 DASZMB010000000
VERSION     DASZMB010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5372736"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="VG272_06845"
     CDS             complement(<1..1050)
                     /locus_tag="VG272_06845"
                     /inference="COORDINATES: protein motif:HMM:NF014346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWF13434.1"
                     /translation="MNKKFSVREVSGLLGILALSMVALGAGASSLYAQAPAAAPPAPA
                     PSAADMKGKTAEQYYKKIEVLKGIPADQVHPAMEYITTALGVGCGYCHVVGHFDQDDK
                     REKHVARSMIQMTMALNSTVFDGKREITCYTCHRGAARAAATMLLANDKMPTEKSATD
                     VFPTLAIKNFSSIDANMSPSKAPATIVTGPAPAPAASKPAAAPLPSVADVFNKYEEAL
                     GGKAAVAKITALNFIGTVDMLVPPPPAPPGTPPAPAAMGTVPAEHDVKGMKGVISVTF
                     PGRPTVSTGYDGNIAWHNTPIREDTGDEKLMLVELGEKFPGLEFREGHTNVQVDAMEK
                     VGDRDTYRVVGTRKDG"
     gene            1399..3123
                     /locus_tag="VG272_06850"
     CDS             1399..3123
                     /locus_tag="VG272_06850"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HWF13435.1"
                     /translation="MRIKELSRQHVHSVALRCGMVAFACMAFGQVSAWADCTTDSVNA
                     LHVANMSIASATLVPAAPPNPEFCDVKGTVTTTGEGAGDGSANFEVMLPARWNQKFIF
                     HGVGGLAGTMTSSANPADQQLFLPRGYATAITDTGHLVANPTWEYTAPGAPDMPKVID
                     YYYRAVHDVTLAAKMLVKSYYHAETIRRSYFDGCSNGGKMGMMEAGRYPADYDGIISG
                     SPWLDPVGTSLWSVKNIKALLDAYIPVAKFQAVSAAILKQCDKLDGVADGLIQNPAKC
                     AFQPDALVPEALTQKQADALKMIMNPVRDEQGKIIFPGASVGNLGQDNITVRGATNYL
                     DAPPADPGAAKPWGDGTLPANWSLAQNILMKLGYYDAGKDMNNEIESNGVVKTDALNL
                     VYTRMADDIPNDPSKLSAYFSKGGKLLIYHGFDDLIISPYSSIWFYEDLAQENGGYPK
                     LQQSARLFMVPGMQHCVLGAGPNSFDTLTAMEQWVEKGIAPDAIPATHLANNVPDRTM
                     PLCKFPEQAHYKGSGDPKSVGNWSCPQNDKSLLTVGPNGIQAGANAAPGARVRPVANT
                     TAKSGNGI"
     gene            complement(3295..4002)
                     /locus_tag="VG272_06855"
     CDS             complement(3295..4002)
                     /locus_tag="VG272_06855"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWF13436.1"
                     /translation="MAEQKRNHARILCRPLAALLTIGALASVFVQSAANAAPSPADKR
                     KPGLYWTLDTDKGKISCKLFETEAPVTVRTMVGLAIGKISYVHPETKQTEQRKFFDGL
                     IFDRVIPGAMIQGGDLLGTGVGHPEGPGFPYKSEIVPSLKFDTPGRLAMANSGPDTND
                     THFFITESPFPSFDGKYTIWGQCENVDVVKAIARAPRDPGDTPTTPVHIQHVTIERVG
                     PAPADAPESTPAGPSSK"
     gene            complement(4350..5420)
                     /locus_tag="VG272_06860"
     CDS             complement(4350..5420)
                     /locus_tag="VG272_06860"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HWF13437.1"
                     /translation="MSRAERFLLSIVVALPLLGVPTATTHAQASYAPPNDLPNPYKTI
                     SDWAKLPNGRKWGATSGVAIGPDGNIWTYERCGGNGCANSDIPPILEFDPSGKMLKNF
                     GAGLFVLPHGIFVDKKGNVWVTDGVGALGTPDDVGKHGKGHQVFKFSPDGKVLMVLGK
                     AGQPGNTHDTFNRPSAVLVAPGGDIFVADGHSSPPESHINARIVKFSKDGKFIKEWGM
                     LGTGHGEFNTPHALALDSKGRLFVADRANNRIQIFTQDGKYLNEWKQFGRPSGIFIAK
                     DDTMYVVDSESRETPGYGNNPGCKRGMRIGSAKTGIVTAFIPDPDPKQSSSAAEGVAV
                     DSHGTIYGAEVGPMDLKKYVKN"
BASE COUNT         1188 a         1471 c         1707 g         1352 t
ORIGIN      
        1 gccgtccttg cgagtgccca ccacgcgata cgtatcgcga tcgccgactt tttccatggc
       61 atcgacttgc acgttcgtgt ggccctcgcg gaattcgagg ccgggaaatt tttctcccaa
      121 ctcgaccagc atgagtttct catctccggt gtcttctcgg atcggcgtat tgtgccaggc
      181 aatgtttccg tcatagccgg ttgatactgt tggcctgccg gggaatgtca cggagatgac
      241 gcccttcatg cctttcacgt cgtgttcggc gggaactgtt cccatggctg cgggcgcagg
      301 cggcgttccc ggaggcgcgg gtggaggagg aacgagcatg tcgacggtgc cgatgaaatt
      361 gagagcggta attttcgcga ctgctgcttt gccgccgaga gcctcttcat atttgttgaa
      421 gacgtccgca acggaaggaa gcggcgcggc ggctggcttc gaggcggccg gtgccggcgc
      481 gggcccggtc acgatcgtcg caggcgcttt gcttggcgac atgttcgcgt cgatgcttga
      541 gaaatttttg atggcgagcg ttggaaaaac gtctgtagcg cttttttcag tgggcatttt
      601 gtcgttcgcg aggagcatgg tcgcggcggc tctggcagcg ccgcgatggc acgtgtagca
      661 ggtgatttcg cgcttgccat cgaacacggt actgttgagg gccatcgtca tctgaatcat
      721 gcttcgcgcc acgtgctttt cgcgcttgtc atcctggtca aagtggccga cgacgtggca
      781 atagccgcaa ccgacgccga gagccgtggt gatgtattcc atcgcgggat gaacctggtc
      841 ggcgggaatt cccttgagga cttcaatttt tttgtaatat tgctcggcgg tttttccctt
      901 catgtcggcc gcactcggcg ctggcgctgg aggagcggcg gccggagcct gcgcgtaaag
      961 gctcgacgca cctgcgccga gcgccaccat ggacaacgct agaatcccca ggagcccgga
     1021 aacttcgcgc acagaaaatt tcttgttcat actttttttg ttgtcctctc gctcgttttt
     1081 atttctggaa cggattcgca tcccacccgg ccaatcctac gcggttgcct gctcggaaaa
     1141 attttgccgt tcgcgcgctg ctggtcgttg gcggtggatt gaatgcgcgg cagcgcttga
     1201 atgtggggct aatcctaagg catggtgagg ctgaaaagca atgatggaac aatgtgcacg
     1261 cggagatcga aaattatgcc gtgtgtttga agcacaaaaa ggagtggtga cattgcggcg
     1321 gtttgggatt aacctcacac caagctgacg accctgacgg tcacaacgtt tcatttggca
     1381 caccatgtgg agggaaccat gagaattaag gagctatctc gtcaacacgt gcattctgtg
     1441 gcgttgcgct gtgggatggt tgctttcgct tgcatggcgt tcgggcaagt ttcggcgtgg
     1501 gccgattgca caaccgattc cgtgaacgcg ctgcacgttg cgaatatgag catcgcgtcc
     1561 gcaactctcg tgcctgcggc gccgcccaat ccggaatttt gcgacgtgaa gggaacggtc
     1621 accacgacgg gcgagggcgc aggagacggc tctgcaaatt ttgaggtgat gcttccggcg
     1681 cgctggaatc aaaaatttat ttttcacggc gtgggcggac tcgccgggac gatgacttcg
     1741 tctgctaatc ctgcggacca gcaactgttc cttccgcgag ggtatgcgac cgcaatcacg
     1801 gacacggggc acctcgttgc caatcccacg tgggaataca cggcaccggg agcgccggac
     1861 atgcccaaag tgatcgatta ttattaccgc gcggttcatg acgtgacgct ggccgcaaaa
     1921 atgctagtga aaagctatta tcatgcggaa acgatccgac gctcctattt cgatggctgc
     1981 tcgaatggcg gaaagatggg gatgatggaa gcgggccgat atcctgcgga ctatgacgga
     2041 attatttcgg gatcgccgtg gctcgatccg gtgggcactt cgctgtggag cgtgaaaaac
     2101 ataaaagcgt tgctcgacgc gtatattcca gtggcgaaat ttcaggcggt gagcgctgcg
     2161 attttaaagc agtgcgacaa gctggatggc gtggcggatg gcctgattca gaatcctgca
     2221 aagtgtgcgt ttcaacccga tgcgcttgtg cctgaggcac tgacgcagaa acaggcggac
     2281 gcactcaaga tgatcatgaa cccggtgcgc gacgagcaag gcaaaattat cttcccagga
     2341 gcatctgtgg ggaatctcgg acaggacaac atcactgtcc gcggcgcaac gaattacctg
     2401 gatgcgccgc cggccgatcc gggcgccgcg aaaccatggg gagacggcac tttgcccgcg
     2461 aactggtccc tggctcaaaa cattttgatg aagctgggtt actatgacgc gggtaaggat
     2521 atgaataacg agatcgagag caacggcgta gtgaaaaccg acgcgctgaa tctcgtgtac
     2581 acgcgtatgg cggacgatat tccgaacgat ccgtcgaagc tcagcgcata tttttccaag
     2641 ggcgggaagc tgctgattta ccacggattc gacgacctta ttatttctcc atacagcagc
     2701 atctggtttt acgaggatct ggcgcaggaa aatggcggct acccgaagct gcaacagagt
     2761 gctcgtctat ttatggttcc cggaatgcag cattgtgtgc ttggggcggg gccaaattcg
     2821 tttgacactt tgaccgcgat ggagcagtgg gtagaaaagg gtatcgcgcc cgatgcgatt
     2881 ccggcgacgc acctcgccaa taacgttcct gaccgcacga tgccgctgtg caagtttccg
     2941 gagcaggctc actacaaagg gagtggtgat ccgaaaagcg tgggcaactg gtcgtgcccg
     3001 caaaatgaca agtcgttgct gacggtgggg cctaatggga ttcaggcggg ggcaaatgct
     3061 gcgccggggg cccgcgtcag acccgttgca aacactactg ccaaaagtgg aaatggaatt
     3121 tagtaggacg tgcttcagcc tgtccgcctt tgattttgct tgcgaaccgt cgttcgttag
     3181 cacccgttgt tacgtgcacg tattgcggca tcgaaacccc ggatgcccac ttcaagacgg
     3241 gcgctacatc atctaaacct acacattggt tacgaggcga ttcgaagtaa tgcgttactt
     3301 ggaagatgga ccggccggcg tggactcggg cgcatcggca ggtgctgggc ccactcgctc
     3361 gatagtgacg tgctgaatgt ggaccggagt ggtcggcgtg tcgccaggat cgcgcggcgc
     3421 tcgcgcaatc gcttttacga cgtccacatt ttcgcactga ccccagatcg tgtattttcc
     3481 atcgaacgag ggaaaagggg attcggtaat aaaaaagtgc gtgtcatttg tgtcgggtcc
     3541 gctgttggcc atggctaagc gtcccggcgt gtcaaatttc aaggacggca cgatttcact
     3601 tttgtacggg aaacccgggc cctcggggtg gccgacgccg gtgcccagaa gatcgccgcc
     3661 ttgaatcatc gcaccgggaa tcacacgatc gaagatgagg ccgtcaaaaa acttcctttg
     3721 ttcggtttgt ttggtctctg gatgtacgta ggagattttg ccaatcgcga ggcccaccat
     3781 ggtgcgcacg gtgacagggg cttctgtctc gaagagtttg caggaaattt ttcctttgtc
     3841 ggtgtcgagc gtccagtaca ggccgggttt gcgcttgtcg gcaggtgatg gcgctgcatt
     3901 cgccgcggat tggacgaata cggatgcgag cgctcctatg gtcagaaggg cagctaaggg
     3961 ccggcataat attcgcgcgt gatttctttt ttgttctgcc actttgctcc gctcctggag
     4021 ttgattgctc taatatacag aaagctcaac agaggacact gagaaatgtg ggatccgctg
     4081 agcgttgggc taaaagttgc gcgcttcttg atcggcgtaa gattggcacg tcagcggcta
     4141 aacaggccgc ggcaaaatgg ttcgcgcgaa tgtgttaagt agcacaggct ctgttgcctg
     4201 tgaggggttt cgcaaagtgc acacagccac gaatggctgt gctgctcgac gattgatcgt
     4261 cattctgagc gtagtgagaa atttctcttc catgtaaagt ctaaaagaga gggcgaccta
     4321 caatcaatcc gcgcgacgtt tttgaggatt taatttttta cgtacttttt cagatccatc
     4381 gggccgactt cagcgccata gatagttccg tggctgtcca cggcgacgcc ttccgcggcg
     4441 ctgctggatt gtttcggatc tgggtccgga ataaatgcgg tgacgatgcc ggttttcgcg
     4501 ctgccgattc gcatgcctcg tttgcagcca gggttgtttc cgtatcccgg cgtttctctg
     4561 gactccgaat ccacgacata catcgtgtcg tccttggcga taaaaattcc gctggggcgt
     4621 ccgaattgct tccattcatt gaggtatttt ccgtcctgag tgaaaatctg gatgcgattg
     4681 ttggcgcgat ccgcaacaaa caaacggcct ttggagtcta gtgcgagggc gtgcggcgtg
     4741 ttgaattctc cgtggcccgt gccgagcatt ccccattctt taatgaattt tccgtctttt
     4801 gaaaatttta cgatgcgggc gtttatgtgc gattcaggcg gtgaactgtg gccgtcggcc
     4861 acaaaaatat ctccgccggg agcaacgaga acagccgacg gacgattaaa ggtgtcgtgc
     4921 gtattaccgg gttggccggc ttttcccagc accatcagga cctttccgtc cggactgaat
     4981 ttgaaaacct gatggccttt tccgtgtttg ccgacgtcat caggagtgcc cagcgcgcct
     5041 acgccatccg ttacccaaac gtttcctttt ttatccacaa aaattccgtg cggaagcaca
     5101 aacaatcctg cgccgaaatt tttcagcatt tttccggagg ggtcgaattc cagaatggga
     5161 gggatgtctg aatttgcgca gccgttgccg ccgcagcgtt cgtaggtcca aatgtttccg
     5221 tcggggccga tggctactcc ggaggtcgcg ccccatttcc ggccgttggg cagtttcgcc
     5281 cagtcggaga ttgtcttgta agggtttggc aaatcgttgg gcggtgcgta gctagcctga
     5341 gcgtgagtgg ttgcggttgg gacccctagc agcggtaagg ccacgactat ggagagtaag
     5401 aatcgttctg cgcgactcat gaattctctt ttcgtgtcgt acggtgaaat gaaaaagaag
     5461 ctctccggcc tgaagaacga ccggccgcta cgaaattcta ctttctctcg atatttacgg
     5521 cgtaatagtc ggataccagc agtgcgagcg cgacgacggt gcctttcaaa aattgagatt
     5581 ccaatgtcaa gcccatcctc gctccacaaa gtttacacgc gtgcctgacg aaatcacgat
     5641 tccgcgccca ggaatatcta ctgagcggga agcggacgcg gcacatgctg cgtcattagc
     5701 acgagcgggc cgccggaa
//
LOCUS       DASZMB010000152         3918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_5094537, whole genome shotgun sequence.
ACCESSION   DASZMB010000152 DASZMB010000000
VERSION     DASZMB010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5094537"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VG272_06865"
     CDS             <1..361
                     /locus_tag="VG272_06865"
                     /inference="COORDINATES: protein motif:HMM:NF025109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HWF13438.1"
                     /translation="THYIEEAEKLCDRVAIVDHGQVIALGSPRELKQRSGAVTRITVR
                     MAKPETNGALGKLEGVTECRAEADDYLLYSTRVPRTIVALVKHLEEQNNELGSLEISA
                     PTLEDIFLELTGRRLRD"
     gene            358..1260
                     /locus_tag="VG272_06870"
     CDS             358..1260
                     /locus_tag="VG272_06870"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF13439.1"
                     /translation="MSQVLGHTWALTVSRMRLAMRNRAFLFFSLIMPIAFLFIYAGIF
                     GRGKSQAVPYLLAEVLALTVMGSFWGLSVQLVTFREQGILRRFRVTPVGASAMLASSL
                     VSNYFLTLPTIAVEFFLSRSVFHMAGLGNLLSIFVLVTIGTVTFASLGLIVASVTNTM
                     QETQIINQIIWFVFLFISGATIPFPMLPGIVQGVAVFLPATYLVSGLQRAMIDHTNLL
                     NLGMYLASLGGCALIAFVVSAQLFRWEPESKAPRQAKLWAAAAVIPFLLLGVWETVNG
                     DLRNHAEQNLQTIRQPYSIERIGH"
     gene            1181..2401
                     /locus_tag="VG272_06875"
     CDS             1181..2401
                     /locus_tag="VG272_06875"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HWF13440.1"
                     /translation="MAICATTPSKTCKPSANRIPSNASAIKKPRGGFVAPGATKSITI
                     RTNQIGLAASQFAPSREADAANRYAIIWLVTCITAQFLHSIAAWRTTMPMKPTEKIWH
                     NGKLIKWDDAKIHVLSHVVSYASAVFEGLRCYETKSGPAIFRVREHMQRMINSGKIYR
                     LDLPYTAEELSTAAAEVVRVNKLNSCYIRPIALRGYGDIGVGGMAVPIEVYMACWEWG
                     RYLGPEAIENGVDVCVSSWNRMAPNTLPSMAKAAANYMNSQLIHMEAHANGYSEGIAL
                     DTNGFVSEGSGENIFVAMDGVLYTPPAGSSVLPGITRSSVITLCEEMKIQVREQMIPR
                     EMLYIADEVFFTGTAAEISPLRSIDRIKVGKGSRGPITKALQEEFFAILDGSKADRHN
                     WLTPVNVPLGAATR"
     gene            2794..3495
                     /locus_tag="VG272_06880"
     CDS             2794..3495
                     /locus_tag="VG272_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HWF13441.1"
                     /translation="MYSKLQKIVAEFIGTFALVFFGVGAITADQFLRSGSTGQPGLGL
                     LGIALAQGLAFGIMVTALGHISGGHFNPAITIGVWVTRKISTFDGIVYCVAQAGGGIA
                     AAYLLRTLPFEAWGSVQLGTPDLAIGMTRATGIIFEAVMTFFLVFVFFATAVDGQGHL
                     QRNAGFAIGLTVTIAVLLGGPFTGAALNPARVLGPAIASNHWTNHGVYWIGPLGGGIV
                     AGWLYDTLFLTKKEE"
     gene            3598..>3918
                     /locus_tag="VG272_06885"
     CDS             3598..>3918
                     /locus_tag="VG272_06885"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HWF13442.1"
                     /translation="MNPARIPLFPLDVVLFPGQAMPLHIFEPRYREMTRHCIQTQTPF
                     GILRASGVNLAQTGCSALIVKVLKEYEDGRSDILTAGQSAFRLIRMHEEKAYMEADIL
                     FLEED"
BASE COUNT          840 a         1095 c         1054 g          929 t
ORIGIN      
        1 cacgcattac attgaggaag cggaaaaact ctgcgatcga gtagccattg tggatcatgg
       61 ccaggtaatc gcccttggct caccacgcga attgaagcag cgatccggcg cggttacgcg
      121 catcacggtg cgcatggcca agcccgaaac aaatggcgcg ctgggcaagc tcgagggcgt
      181 aaccgaatgc cgcgccgaag ccgacgatta tctgctgtac tcaactcgcg tgccgcgcac
      241 catcgtggcc ttggtaaagc atctggaaga acaaaataat gagttgggta gtctcgaaat
      301 ttccgcaccc acgctcgaag atattttcct cgagttgacg gggagaaggt tacgcgattg
      361 agtcaagtct tgggtcacac ctgggcactt acagtaagcc gcatgcgcct cgccatgcgc
      421 aatcgagcgt tcttgttttt cagcttgatc atgcccatcg catttttatt catttacgcc
      481 ggcattttcg ggcgcggcaa atctcaggca gttccctatc tgctggcgga agttttggcg
      541 ctaacggtga tgggcagctt ctggggactt agcgtgcaac tcgtgacatt tcgcgagcaa
      601 ggcattctgc gcaggtttcg cgtaactcct gtaggcgcga gcgcgatgct cgcttcgagc
      661 ttggtttcca attacttttt gaccctgccc acgatcgccg ttgagttttt cttgtcgcga
      721 tcggtattcc acatggcggg attgggcaat cttctctcga tttttgtttt ggtaactatc
      781 ggtaccgtga ccttcgcttc gctcggtctg atcgtcgcca gcgttacgaa cacgatgcag
      841 gaaacgcaga tcatcaacca gattatctgg tttgtgttcc ttttcatttc aggcgcaacg
      901 attccctttc ccatgctgcc tggcatcgtg caaggtgtgg cggtctttct tccggctacg
      961 tatctcgtaa gcgggctgca acgcgccatg atcgaccata cgaatctcct gaacctcgga
     1021 atgtatttgg cttcgctcgg gggatgcgcc ctgatcgctt tcgtggtctc cgcgcagctt
     1081 ttccgctggg agccagaatc caaagcgcca cgccaggcca aactttgggc tgcagccgcc
     1141 gtcattcctt tcctgctgct cggcgtctgg gaaacggtga atggcgattt gcgcaaccac
     1201 gccgagcaaa acctgcaaac catccgccaa ccgtattcca tcgaacgcat cggccattaa
     1261 aaaaccgcga ggcggttttg tggcgcccgg cgctacaaaa tcaatcacga ttcgcaccaa
     1321 ccaaattggc cttgccgcat cgcaattcgc gccatcacgc gaagccgatg ccgcgaaccg
     1381 ctatgctata atttggctgg taacctgcat tacagcacaa ttcctgcatt cgattgcagc
     1441 ctggagaact acgatgccga tgaagccgac ggaaaagata tggcacaacg gaaagctgat
     1501 caaatgggat gacgcgaaaa ttcacgtact ttcccacgtc gtcagctatg cctcggcggt
     1561 cttcgaaggt ttgcgctgct acgaaacgaa aagcggtccg gcaatcttcc gcgtgcgcga
     1621 acacatgcag cgcatgatca attccgggaa gatttaccga ctcgacttgc cctacacggc
     1681 ggaagaactt tctactgctg ctgctgaggt agtccgcgtg aacaaactga attcctgcta
     1741 catccgcccc atcgcgctgc gcggatatgg agacatcggc gtgggtggca tggctgtccc
     1801 catcgaagtt tacatggcgt gctgggaatg gggacgttac ctcggtccgg aagcaatcga
     1861 aaacggagtg gacgtctgcg tgagctcctg gaaccgcatg gcgcccaaca cgctgcccag
     1921 catggccaaa gccgccgcga actacatgaa ttcgcaactc attcacatgg aagcgcacgc
     1981 caacgggtat tccgagggca tcgcactcga tacgaatggt ttcgtgagcg aaggttccgg
     2041 cgaaaatatt ttcgtcgcga tggatggcgt gctgtacacg ccgcccgcgg gttccagcgt
     2101 gcttccgggt atcacccgca gctcggtcat cacgctatgc gaagaaatga agattcaggt
     2161 tcgcgagcag atgattcccc gcgaaatgct gtacattgcc gacgaagttt tcttcaccgg
     2221 gacggccgcg gaaatcagcc ccctgcgatc cattgaccgg atcaaggtgg gcaagggatc
     2281 gcgcggtccg atcaccaaag ccctgcagga agaattcttc gcgatcttgg acggaagcaa
     2341 agcggatcgt cacaactggc tcacgcccgt gaacgtgcca ttgggcgccg ctactcgctg
     2401 acgaatatat tttttcacca gccccattca gacaaacatc cggacataat taaattgtcc
     2461 ggatgtttta tttcctgagt catttgcaca tcgtttttgt attgatttga attgcacacg
     2521 cacaaaagct gcccgccaaa aatcgggcgg cctctacaaa agcaacggca gaccggtttt
     2581 gtagaggcgt ctccgtcccg ttcacagaga acgttctcgg tgagccgcgt tcttcgggac
     2641 cgatagggcg acttctcact caaattaagg cgctaacttg tacatcgaaa aagattgatg
     2701 ctttgactcg cccgcttccg ttctcttaca atcttcctcc ccatcgaaaa agagggtggc
     2761 cgtcctctgg cgggtccgcc gggagaaata ttcatgtatt cgaagctcca gaaaattgtc
     2821 gctgaattta tcggcacctt cgccctggta ttttttggcg tgggcgcaat taccgccgat
     2881 caatttttgc gctccggctc gaccggccag ccgggcttgg gtttgctagg catcgcgctg
     2941 gcgcaaggcc tggcattcgg aatcatggtg accgcgcttg gccacatttc cggagggcat
     3001 tttaatcctg ccataacgat tggtgtttgg gtcacccgca aaatctccac gttcgatggg
     3061 atcgtctatt gcgtcgcaca ggctggtgga ggcatagcgg cggcttacct cttacggact
     3121 cttcctttcg aggcttgggg ttccgtgcaa ctgggaacgc cggacctagc cattggcatg
     3181 acgagggcca ccggaattat ttttgaagcc gtgatgactt ttttcctggt gttcgtattt
     3241 ttcgctactg ccgtggacgg gcagggtcac ctccaaagga atgcaggctt cgcgatcggg
     3301 ctgaccgtga cgattgcagt cttgttggga ggccctttta caggcgctgc gctgaacccc
     3361 gctcgcgttt tgggccccgc gattgcctcc aatcactgga cgaatcacgg cgtctactgg
     3421 attggccctc tcgggggagg cattgtggcc ggctggttgt atgacacctt gttcctcacg
     3481 aagaaagagg agtaagtttc cgcttccgtc agtgagcgca aagcgggtct ccaaataaat
     3541 tatagaattc cgcggggaat tttttgagcc ttctgctatg atttcccgcg gagatcaatg
     3601 aatcccgcgc gcattccact ctttccgctc gacgttgtgc tcttccccgg ccaggcgatg
     3661 cccctgcata tttttgagcc gcgctaccgg gaaatgacgc gccattgcat tcagacacaa
     3721 actccattcg gcattcttcg cgctagcggg gtgaatctcg cgcagacggg ctgctcggca
     3781 ctgatcgtaa aagtgctcaa ggaatatgaa gatggccgct cggacattct aaccgccggc
     3841 caaagcgcgt tccgcttaat ccgcatgcac gaagaaaaag cttacatgga agccgacatc
     3901 ctatttcttg aggaagat
//
LOCUS       DASZMB010000153        21661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2625155, whole genome shotgun sequence.
ACCESSION   DASZMB010000153 DASZMB010000000
VERSION     DASZMB010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 21661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21661
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2625155"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VG272_06890"
     CDS             <1..678
                     /locus_tag="VG272_06890"
                     /inference="COORDINATES: protein motif:HMM:NF044477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWF13443.1"
                     /translation="GLLYELASVAMAIPALTEEERTHSEAMISGAMSYAHKVRCDLKH
                     SEQVRSLALQLFDQLWPYHQLGADSRGILASAALLHDCGVLINRKGHHRHGEYLIHNA
                     KIPGLLGWQKNMTAALVRYHNKKSEPTAKHSVFASLSREQRRKTRLLAGMLRLAECLE
                     SDHRQVISKLTIEGGPRDVRAHVEMRDSSRLNLVGIQRKARLLERELKLRLSFQRVMV
                     VEKKRVA"
     gene            678..1178
                     /locus_tag="VG272_06895"
     CDS             678..1178
                     /locus_tag="VG272_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWF13444.1"
                     /translation="MMHLYLIRHGIAVDREDPNCPPDTERPLTPKGMKRSRAAALGLR
                     ALDVKPDVVLTSPWLRAAQTAEIFCETIGYPAKKIISTDALKGTSAPHDLMRELQSVK
                     ADVVFCFGHEPHLHQVIGYILHTHAKITELKKAGIAVLDLARFAPPHGRLLALYPPST
                     LRLLAK"
     gene            complement(1193..1789)
                     /locus_tag="VG272_06900"
     CDS             complement(1193..1789)
                     /locus_tag="VG272_06900"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HWF13445.1"
                     /translation="MPDRIRAGAEEPPRPTCHWARSGILAHYHDVEWGVPVHDDRLLF
                     EFLILEGAQAGLSWETVLRKREHYREVFDGFNASRIAGYDARKVRALLGDAGIIRNRL
                     KISSTISNAQAFLEVQSKIGSFDSYIWQFTSGKTLRPKRRVPKDIPAQTVESDAMSKA
                     LKKLGFRFVGSTICYAFMQATGMVNDHLQTCFRWKEII"
     gene            complement(1796..2131)
                     /gene="trxA"
                     /locus_tag="VG272_06905"
     CDS             complement(1796..2131)
                     /gene="trxA"
                     /locus_tag="VG272_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HWF13446.1"
                     /translation="MADNVNSVTDSTFQSEVIEASKTQPVMVDFWAEWCRPCLMLAPT
                     VAEVATAYAGKIKVVKLNVDENTNSAGQFNIRGIPTLLIFKGGQVAEQVVGAVPRDQI
                     EKAVQRHIV"
     gene            complement(2121..3656)
                     /gene="glgA"
                     /locus_tag="VG272_06910"
     CDS             complement(2121..3656)
                     /gene="glgA"
                     /locus_tag="VG272_06910"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HWF13447.1"
                     /translation="MKILFVASEGLPFSKTGGLADVIEALPKSLVALGHEVSVLLPRY
                     RKTKVVGVSEPSMTVPMGDGLRFPAIVEGGAIHGVYFYFVDDPEYFDREQLYGIAGKD
                     YPDNAERFAEFCGAAIEFCKHVWMPDVIHCHDWQSALVPVLLRTRYVEDRALAELSVV
                     FTIHNLGYHGAFPKEVMDKIGLPERLFTIDGMEFFGQVNFLKGGLIYSDYLTTVSRKY
                     AEEIKTVEYGQGLDGVVRNRADRLTGILNGVDYSAWSPERDKVIASRYSAKDLSGKLA
                     CKKDLLQAFGLPQEAVSKPVIGIVSRFAAQKGFDLIEKIAPELLAEELVITALGAGEP
                     KYEKLFRELAKAFPSKFNVKVAYDNIVAHKIESGADVFLMPSRYEPCGLNQIYSLKYG
                     TVPVVRATGGLDDTIDQFDITTGRGTGFKFQAYDSSALLECIRQAISVYRNEPPVWRK
                     IQSNGMAQDFSWQASAKEYVKVYEAAWNEGIQKGISSSNVLRAKSAKMTPVLPIGKGS
                     HGR"
     gene            3951..4427
                     /locus_tag="VG272_06915"
     CDS             3951..4427
                     /locus_tag="VG272_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWF13448.1"
                     /translation="MATVKTIPDGYHSVTPYLYVKGAVAAIDFYKEVFGATELMRALG
                     PNGKIMHAELKFGDSIVMLADEEPTRGILSPLTVGGFSVGFHLYVKNSDAIMKKALEN
                     GAKELHPMKNQFYGDRSGSLLDPFGHMWSVATHVEDVSPEELKKRMASAMSQSAGA"
     gene            4647..5420
                     /gene="rsmA"
                     /locus_tag="VG272_06920"
     CDS             4647..5420
                     /gene="rsmA"
                     /locus_tag="VG272_06920"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HWF13449.1"
                     /translation="MSPERLGQHFLADANWQERIVDDIHIDNGRWVEIGAGHGEMTTR
                     LAQRASKLYAVEVDPSLARRLRQVTAPFKNVEIVHSDVMAVDFEKLTSGERFSVYGNL
                     PYYITSPILHRLFEHADRIAAIHIVIQYEVAVRIMARPGRRDYGYLSVASQWYGKPEI
                     AFRIPPGAFNPPPKVDSALVSFHMPGERAKIRVSNEHAFLEFVKECFAQKRKTLRNNL
                     RARLGTRTEEILEAANLPSDARAEQLTISQFAVLFPLAL"
     gene            complement(5497..6246)
                     /locus_tag="VG272_06925"
     CDS             complement(5497..6246)
                     /locus_tag="VG272_06925"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HWF13450.1"
                     /translation="MESKSIWTIAHRGASGHAPENTMVAFRRAVELGARFIETDLQLS
                     RDARVIAMHDFTLDRTTTGKGDVHQLPLNQIRVLDAGAWFGDRGAKSYAGEKVPTLEE
                     ILDFAKQNDVIFYLEIKAGQQWGIEHVVVATLRDYNAGARVVILSFDPTALDTVHRLD
                     STMMTGFLCEHPASDLVERTVRAGARQLVARGDLITSEVIEKAHHAGLQVVAWTINEV
                     EQMQRLIAIGVDGIITDYPDRLLGVLQEPGK"
     gene            complement(6644..7108)
                     /gene="nrdR"
                     /locus_tag="VG272_06930"
     CDS             complement(6644..7108)
                     /gene="nrdR"
                     /locus_tag="VG272_06930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HWF13451.1"
                     /translation="MKCPFCSVMEDKVIDSREGRAGDLIRRRRECLKCGRRYTTYERI
                     DEIPYMVIKKDGRRERFDRQKILQGLLKSCEKRPVATAKLEALVDEVERFVHESPERE
                     RKTSEVGELLMNRIKKLDKVAYVRFASVYLDFKDVKEFMSELKGLLKDRSKK"
     gene            complement(7108..8262)
                     /locus_tag="VG272_06935"
     CDS             complement(7108..8262)
                     /locus_tag="VG272_06935"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HWF13452.1"
                     /translation="MRGRLRRILIALIILAGLVVVAYQSRHKVHLADFTWNKFAASVG
                     QANVLLLLLCLIGIYGCYAIRAVRWQRFCKYVGPTTFLNTYSGTLMGFAAIFVLGRAG
                     EPVRPLLLARKERLPVASLFGIFFLERFCDFAAAAALACLALLVFPSRLSDAGADMNW
                     VEGARHGGWLLFGAMTALIILLVVYRMHGAAILDRILIRWRDAGGFRSRFTSAITGIS
                     EGLQAIRTTSDLIAAIFYTLAHWVLATLIYLGVAKSFGASFLHSEMNFSGAMLLLAVT
                     LVGSVLQLPGVGGGAQIASFIGLTKIFGVEQEPAAAMAVVLWIITFAGGTLAGIPLLI
                     HEGMSVGELRQLARAEAEAEESGKHIGVTQQNGSSPSSSAAKQKPLGEKP"
     gene            complement(8510..9802)
                     /gene="aroA"
                     /locus_tag="VG272_06940"
     CDS             complement(8510..9802)
                     /gene="aroA"
                     /locus_tag="VG272_06940"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HWF13453.1"
                     /translation="MSKQIIRPARTISGSLEVPGDKSISHRYAILASLAEGPSEITHF
                     SGAADCRSTLDCFSQLGVKIDIEGDRVRIAGTGLDGLRKTRRALDAGNSGSTMRMLAG
                     ILAGQDFKSTIDGDASLRRRPMRRIMDPLTQMGARIHSREGGFPPLKIDGTKLVAIEY
                     SLPVPSAQVKSAVLLAGMFAEGLTSVTEPVRTRDHTELALAEFGARLDHEGHTIRIHG
                     RPRLKGRSLAVPGDLSSAVFFLAAALVLPESNLVIHNVGLNPTRSAVLDVLGSMGAPI
                     SLVSIRSEHGELVGDVSIRHESLKGGSIEGDVIAQLIDELPAIAVLGPYTEQGIEIRN
                     AAELRVKESDRIAVLAENLRRMGAQVEELPDGLRVAGWSAGRLHGAEIEPHGDHRMAM
                     AFAVAALGAEGETVIRDAECAGVSYPEFFSTLERMVER"
     gene            9988..10617
                     /locus_tag="VG272_06945"
     CDS             9988..10617
                     /locus_tag="VG272_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13454.1"
                     /translation="MKYFIGLALAALLAIAPAFAQAPKGWKMHMDHSTDASDPDAAGA
                     NKFTVSGSGFHAVNPMAAVYWNPENTMTGNYTLKGTFKLIKSSGHDEYYGLVFGGSGL
                     EGAAQSYIYFMITDDGTYLIKRRDGSSTQGISQKTPNAAVKKPDASGTATNNLEVRVK
                     ADKIDFAINGTVVTSLPKTGAAAKTDGIYGIRINHQLDVQIDGFAASKM"
     gene            10730..11272
                     /locus_tag="VG272_06950"
     CDS             10730..11272
                     /locus_tag="VG272_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF13455.1"
                     /translation="MHRTKTEIVDRPLTLCSKWPMPSDREKEAKQRFQDAFEAQMAGE
                     LDRAVTLYLRSLALFPTAEAHTFLGWTYHFQGKIEQAIAECKRAIKIDPDFGNPYNDI
                     GVYLIDLDRHDEAIPWLEQAIIAKRYEPRHFPYYNLGRIYLAKGLLNRACELFQQSLN
                     VEPRYSLARVALENARRMVN"
     gene            11477..12052
                     /locus_tag="VG272_06955"
     CDS             11477..12052
                     /locus_tag="VG272_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13456.1"
                     /translation="MKMLLVAMIFFQAMQGAPLVDNEFVRVIKNSAPCAPAAPSCGDR
                     IVVALGPIEINGQKLERGEVKGFKAGERYSPPKDGNFLEVSIKPTHPKVMSPGAGTPA
                     APHNKVLYDGKDITVFEEKMQPDEYDPPHSHNVRLAIFLNDTMVEQWTEGKDETRELI
                     PDVVTWRPAVVHASKDVGKVPIHNILIEFKP"
     gene            12063..13262
                     /locus_tag="VG272_06960"
     CDS             12063..13262
                     /locus_tag="VG272_06960"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HWF13457.1"
                     /translation="MKRSTNRILTTHVGSLIRPLRLLELVRTRESGNAADVHAYEQCL
                     KESVAEVVKQQAHAGIDIVNDGEFGKSTSWALYALKRVSGFEMRPVKPGANPFARGAD
                     RERFKEFYAELESGNDRTWSNVTKQEAVCVAPVKYTSLAELQDDIDNLKAALQGAKVE
                     EAFLPVAAPSSAIPDRKNEYYKNEEELVVALAEALRTEYRAIVDAGFLLQVDDARAAV
                     TYDRMVPPASFEDYYKWVGWHVEVLNHALEGIPEDRIRYHVCWGSWPGPHTTDVPLRK
                     IVNLILKVRAGAYLIEAANPRHEHEWRVWKDVKLPEGKILAPGVVSHGTNVVEHPELV
                     AERITRFANLVGRENVIASTDCGFAQEQFNRRVHPSIMWAKLEAMAEGARIASDELWG
                     RAAAERA"
     gene            13406..13846
                     /locus_tag="VG272_06965"
     CDS             13406..13846
                     /locus_tag="VG272_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWF13458.1"
                     /translation="MKILIAIDGSEFSQAALRSVIARPWNAGTEVKVLHVVEPPSLLI
                     GREMGPYDPEFETVWKAVREQAKDLVEKATEKLRAAGFQVSTQLVEGNPKSQIIDAAN
                     EWHADMIILGSHGWSGLNRFLMGSVSEGVVRHAHCSVEIIRTAS"
     gene            complement(13888..15621)
                     /locus_tag="VG272_06970"
     CDS             complement(13888..15621)
                     /locus_tag="VG272_06970"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HWF13459.1"
                     /translation="MVKHIFSRRDRLRTDFAGAASTTVPAGGLASSTLLRYALFLFCA
                     LAINIIPTAAADCANLTSLQLKDTTISSAAVIPASGMIPEYCKVLGNIHNLPQSTILF
                     EVSLPMTKWNGKYFVAGGGGYNGVIPKLTQALAEGYAAAGSDTGHEAKDVNWALNNLD
                     AQNNYAYLATHVVTSIGKQILRAFYSQHERRAYFVGCSNGGKMALAEIQRYPDDFDAA
                     ISGDPVIDRTKLMMQYAWNAQALAPAPIPPAKIPVIEKATMSACHESGGAINGLLMTP
                     ARCKFEPKMMVCPSGDGPNCLTAGQARALQKILEGPVSSTGQELHPGFVPGHEDDYQV
                     FLTGTGTDAAKPASTWALSDIFMRQFVFGANFDLVKQFKFDASLAALVPLAVAQDNAN
                     PDLSEFKAHGGKLLMYHGWADHSITPLRSIEYYEDVIETMGKEYAEVQGEPNAYAVMD
                     FMRLFMVPGMHHCGNGPGPNVFGGANRGLPPQLDAQHDIVMALDRWVEDGVAPEKIIA
                     SHLTNGAVDRTLALCPYPQTPIFNGSGDANVAENYHCEDRSFRWAIAGVQNSKLKKDQ
                     TTPPRTVARPN"
     gene            15813..16283
                     /locus_tag="VG272_06975"
     CDS             15813..16283
                     /locus_tag="VG272_06975"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWF13460.1"
                     /translation="MYRIVKGDLSDPRVIELLQTHVTRARAETARGSAHALDVTALRT
                     PDISLWTIWDDESLLGAGALKRLSADHGEVKSMYTVESARGKGVGSAMLRHIIATARA
                     SGMSRLSLETGSWDYFQPAREFYKRHGFQECPPFGDYLLDSNSVFMSMDLGEAE"
     gene            16470..17243
                     /locus_tag="VG272_06980"
     CDS             16470..17243
                     /locus_tag="VG272_06980"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWF13461.1"
                     /translation="MRIARHMPFVIAAMAMMLLASAAQAATKSQWIDYKQGNTALSGF
                     LVYDDAVPGRRPGVLMIHDRSGFSAGTLADAEMISKLGYVVFAEDIFGKGVVPKTVPE
                     MMDTIAIYDKDRALMRARALAGLEVLKSQPMVDPAKLASVGYCFGGTTGMELIETGAP
                     ILGFVSVHGAFRDFTPEGAKNIKGRVLILHGAEDPVAPIEELNAVISQLRGAKVDFEV
                     HLYSGAAHAFTRPQNPSEVRADDEYKVAMTRFLKDNLAH"
     gene            complement(17245..17769)
                     /locus_tag="VG272_06985"
     CDS             complement(17245..17769)
                     /locus_tag="VG272_06985"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HWF13462.1"
                     /translation="MRSLIRIGRISLLAALICAHGPHAQSVRTIFFVRHADKVSDDTD
                     APLSDAGNRRAKCLANMLAEAQIDQIFVSDLQRTQQTAAPLAEKLHQNTTVIPLSTPD
                     RLVEAIRLGTASNVLVVWHDMTLPKIMQALGAPAVAPIGHTEYDRFFILTLTDNKANP
                     QPRFTALRYCDSAE"
     gene            complement(17921..20794)
                     /locus_tag="VG272_06990"
     CDS             complement(17921..20794)
                     /locus_tag="VG272_06990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWF13463.1"
                     /translation="MRRGIYFLLLSSFLFHVSGLRPVSAQDGKGSISGTVKDSSGGAL
                     KGALVELDPTGAKTVSDEQGQFRITDVAAGQYTVTVSYVGLANFTKAVQVIAGQGAST
                     DAVLNVAAVSDQMVVTADRVQGEAESINVERTADNIIQVLPAEVIRSLPNANMADALG
                     RLPSVTLERDEGEGKYVQVRGLEPRLTNTMIDGMDVPSPESGVRQIKFDSIPADIVES
                     VEINKTLLPNMDGDGIGGSVNLITKSATDQPTINLSGMGGFMPILGGRGQVETTGTIG
                     QRFGSQKRFGALIGGSYDWTGRGIDDVEPTPDIATLPGGGTERFFDTADMREYRYYRS
                     RWGLAGSLDYRLTEGSNLYAHGLFSNFKNYGDRFDYGINDNTPGVTTLNGNGGTPSFG
                     TQIRRPDISVGSVLIGGRHDFNTILVSWNLSAGRASLINQGDGLASFGSTLPGSSCQF
                     SPSLTTNFYEPQFTPVCFTEAFNTSAYALNQITINHGLTAQLNLQFDGEMAKRYHIGS
                     HPATFEFGGKFRNAHKFDDAYSITTTPNSGVSIPVSQFPNDLTNNNYYGGAYKLGPNP
                     AFQDILNFANANPSEFTIASSQGVDPSNYDLVEKVSAGYIMNTVDLTSKLRVVGGIRF
                     EGTNLSTVSFDQVANTLSFKANGSYLSILPSAAIRYAVTPSTNFRLSYSRGLSRPDPQ
                     DIAQAVTYTTIGSPGIQQNTVNLGNPNLKAETGDSIDFLVEHYLNSFGMISVGYFYKN
                     LTNPIVQNQFFLNNFSPNPVIPPQTFLATQPINAGSAWLNGVEFAYLQHLSFLPGPFK
                     GLGISANYSYVTSRASGLPGRSDHPLLLRDAPNTWNISPTYDMGRFSVRVGLSYNQAN
                     ISSYAYQDGSLLADGVTHSTPTPGGINGPLSDQYFYTHLQVDAQGSIRLAHGFTFVMY
                     GLNLTNEVFGFYQGSTQYQVQREFYEPTVSAGFRWSPEREKRP"
     gene            21398..>21661
                     /locus_tag="VG272_06995"
     CDS             21398..>21661
                     /locus_tag="VG272_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13464.1"
                     /translation="MKKRLRITWFLAACLCMPLVSSAQSPPPRAVVSVRELSIPPRAV
                     HEFQKGIDRLKKEDANGSLPHFERAVADFGDYYEAYFEIGIAEL"
BASE COUNT         5204 a         5942 c         5685 g         4830 t
ORIGIN      
        1 ggacttcttt acgaacttgc ttccgtcgca atggcgattc ctgcgcttac ggaagaggaa
       61 cgaacacatt ccgaagccat gatctccggc gccatgtcct atgcgcacaa agttcgttgc
      121 gacctgaagc attcggagca ggttcgcagc ctggctcttc aactttttga ccagttgtgg
      181 ccgtatcacc aattgggggc ggattcccgg ggtattcttg catcggccgc cttgcttcat
      241 gattgtggag tgctcatcaa tcgcaagggc catcatcgcc atggcgaata tctgattcac
      301 aatgcaaaaa ttccggggct cctcggatgg cagaaaaaca tgacggccgc cctcgtgcgc
      361 tatcacaata aaaaatcgga acccactgcc aagcattccg tcttcgcatc actaagccgc
      421 gaacaaagaa gaaagacgcg ccttctggca ggaatgctac gcctcgccga atgcctcgaa
      481 agcgatcacc gccaggttat ttccaaactt acgattgaag gcggcccgcg cgatgttcgc
      541 gcccacgtgg agatgcggga ttcttcgcgg ctgaatttgg tggggattca aagaaaagcg
      601 cgactgctcg aacgggagct gaaattgcgt ctatcgtttc agcgggttat ggttgttgag
      661 aagaagaggg tcgcataatg atgcacttat atttgattcg tcacggcatc gccgtggacc
      721 gggaagatcc taactgcccg ccggatacgg agcgcccact gactcccaag ggaatgaaac
      781 gcagccgcgc cgcggccttg ggtttgcgcg ctctggatgt aaaaccggac gtcgtgctca
      841 caagtccgtg gctcagagcc gcacaaaccg ccgaaatttt ctgcgaaacg attggctatc
      901 ccgcaaagaa aattatttcc acggacgcat taaaaggcac aagcgcgccg cacgatctaa
      961 tgcgcgaatt gcagtcggtg aaagccgacg tggtattttg cttcggccac gagccgcatc
     1021 tgcatcaggt gatcggctat atccttcaca cgcacgcaaa aatcacggag cttaaaaagg
     1081 cgggcatcgc agttttggat ttggcgcgct tcgctccccc tcatggaagg ttgctggctc
     1141 tttacccccc aagcacgctg cgactgctgg cgaaatagca ccagtggcaa attcaaataa
     1201 tctctttcca ccgaaaacaa gtctgaagat gatcgttcac catgcccgta gcctgcataa
     1261 aggcgtagca aatcgtggaa cccacaaaac ggaaacccaa ttttttaaga gccttgctca
     1321 tggcgtcgga ttccacggtt tgcgcgggaa tatccttggg gacgcgccgc ttcgggcgca
     1381 aagtttttcc gctggtgaat tgccaaatat aggagtcaaa gctgccaatc ttgctttgca
     1441 cttcgagaaa tgcctgcgcg ttagaaatcg tagaggaaat ttttaaacgg tttcggataa
     1501 ttcccgcatc gccgagcaag gcgcgaactt tacgtgcgtc atagcccgca atcctggatg
     1561 cgttaaatcc atcgaacact tccctgtaat gctcacgttt tcgcaggacc gtttcccaac
     1621 tcagcccggc ctgcgcgccc tcaagaatta aaaattcaaa gagcagccga tcgtcgtgca
     1681 caggcacacc ccattcaaca tcatggtagt gcgccaaaat tccgctgcgc gcccaatgac
     1741 acgtcggccg aggcggttct tccgccccgg ctctgattcg atctggcatg gtgaattaaa
     1801 cgatgtggcg ctgtacagct ttttcgatct ggtctcgggg aacagctccg acgacttgtt
     1861 cggccacttg cccgcccttg aaaatcaaga gggtcgggat accacgaata ttgaactggc
     1921 cggccgaatt cgtattctcg tccacgttca acttcacaac tttaattttt ccggcatacg
     1981 cggtggcgac ttcggccacg gtcggcgcca gcatcaagca aggcctgcac cattctgccc
     2041 agaagtcgac cattaccggt tgcgtcttgc tcgcttcaat cacttctgac tgaaacgtgc
     2101 tatcggttac actatttaca ttatctgcca tgagaaccct ttcctatggg aagtaccggc
     2161 gtcattttag ccgattttgc ccgcaacaca ttagatgaac ttatgccttt ttggattcct
     2221 tcgttccagg ccgcctcata cacctttaca tactccttcg cggacgcttg ccatgagaaa
     2281 tcctgagcca tgccattcga ctgaatcttg cgccagacgg gaggttcgtt ccgatagacg
     2341 gaaattgcct ggcgaataca ttccaataat gcactactat catacgcctg aaacttaaat
     2401 cctgtgccgc gccccgtcgt gatatcgaac tggtcaatcg tatcatccag cccgcccgtc
     2461 gcccgaacca ccggaaccgt tccgtacttc aaactgtaaa tctgattcaa tccgcatggt
     2521 tcgtagcgcg aaggcataag aaacacgtcc gccccagatt caatcttgtg cgctacgata
     2581 ttgtcgtaag ccactttcac gttgaatttg gacggaaaag ccttcgccaa ttcacggaat
     2641 agcttctcgt atttcggttc gcccgctccc agtgcggtaa taaccagttc ctccgcaagc
     2701 aactcgggcg cgattttctc aatcagatca aaaccttttt gcgcggcaaa tcgcgagacg
     2761 atcccgatga ccggtttgct gaccgcttcc tgcggcaggc caaatgcttg cagcagatct
     2821 tttttgcagg cgagctttcc ggaaagatcc ttcgcggaat atcgcgaggc aatgaccttg
     2881 tcgcgctccg gactccacgc cgagtaatcg acgccattta gaattcccgt caagcggtcc
     2941 gcgcgattac gcacaacgcc gtcgagtcct tgtccgtact ccacggtttt tatttcttcc
     3001 gcatatttcc tgctgacggt cgttagataa tccgaataaa tcagcccgcc cttcaggaaa
     3061 ttaacctgtc caaaaaattc catgccatcg atcgtgaaca agcgttccgg tagcccaatt
     3121 ttgtccatta cttctttcgg gaatgctccg tgatatccca aattgtgaat ggtgaaaaca
     3181 acggacaatt ccgccagcgc tcggtcttcc acatagcggg tccgcaacaa tacgggaacc
     3241 agcgccgatt gccagtcatg acaatgaata acgtccggca tccacacgtg cttgcagaac
     3301 tcgatcgccg ctccgcagaa ttcagcaaag cgctcggcat tatccgggta atcttttccc
     3361 gcaatgccat acaattgctc gcgatcgaaa tactcgggat cgtccacaaa ataaaaatag
     3421 acgccgtgaa tcgctccgcc ttcaacaatc gccggaaagc gcaaaccgtc gcccatcgga
     3481 accgtcatgc tgggttcgct gacacccacc accttcgtct tccgataacg cggaagaaga
     3541 acgctgactt catggccgag cgcgaccaaa gatttaggca aggcctcgat gacatccgcc
     3601 aggccgcccg tctttgaaaa tggaagtccc tctgatgcca caaaaagtat tttcataccg
     3661 atccgaggta tccaacctac tgaaaagcct cctcatgcgt caaggacggc caggtatttt
     3721 caattcgtgt caggttccgc gatgaaattc ctggggaaac gtatcgaaaa tcaactgtct
     3781 accccatcgg agtttacgaa cgaaaaggac tgaaagcgaa atcttccaca gccatgcagc
     3841 gctcttcgat ttttggatgt agaatgaatt tgcaataccc agccaaactc ggcggtgcct
     3901 tgaacaacct gaggtccgct agtttattcc catacaagaa aggcgaaatc atggcgacag
     3961 taaaaacaat tcccgacgga tatcattctg ttacacccta cctgtacgta aaaggcgcag
     4021 ttgccgcgat tgatttttat aaagaggttt ttggggcgac tgaattgatg agggcgcttg
     4081 gccccaacgg aaagatcatg catgcggaat taaaattcgg cgattcgatc gtaatgcttg
     4141 cggatgaaga accgaccaga ggcatcctga gtcctctcac ggtcggagga ttttccgttg
     4201 gctttcacct gtacgtgaaa aattcggatg caatcatgaa aaaagcattg gagaatggag
     4261 cgaaagaact tcatcccatg aaaaaccaat tttatggcga tcgttctgga tcattgctcg
     4321 atccatttgg gcacatgtgg tcggttgcga cccacgtgga ggacgtctcg cctgaagaat
     4381 tgaagaagcg aatggcgtca gcaatgagcc aatcggcggg cgcctaaacg cagcgaggaa
     4441 tccctcagcc tttagtagcg cgggcttcag cctcgcgaat tttgtccttg caaggacgag
     4501 ttcgcgcaga ccagatgaag cccgcgccac aaagcaaaga gaaatccccc cactctgcct
     4561 ttagaatgaa ttcatacccg ggcaattatt tccctatcca cgatccgtgc tgtgcatgcg
     4621 cttctctctt gctacactct tgtcccatgt cacctgaaag gcttggacaa cattttcttg
     4681 ccgacgcgaa ttggcaggaa cgaattgtgg atgacattca catcgataac ggaaggtggg
     4741 tggaaatcgg cgcgggccat ggtgagatga cgacacggct cgcgcaacgt gcgagcaaat
     4801 tgtacgccgt cgaagtcgat ccttcgcttg cccgacgttt gcgccaagta accgcgccat
     4861 tcaaaaacgt ggaaattgtc cacagcgacg tcatggccgt ggattttgaa aaattgacga
     4921 gcggtgagcg gttttccgtc tacggaaatt tgccctatta catcacgtcg ccgattctgc
     4981 atcgcctgtt cgagcacgcc gatcgtatcg cggccattca catcgtaatt cagtacgagg
     5041 ttgcagtaag aatcatggcg cggcccggac gccgcgatta tggctacttg tcagtagcgt
     5101 cgcagtggta tggaaagccg gagatcgcgt ttcgcattcc tcccggagcc ttcaacccgc
     5161 caccgaaagt cgattccgca ttggtcagtt tccacatgcc cggcgagcgc gcgaaaatca
     5221 gagtctcgaa cgaacatgcc tttctggaat ttgtaaaaga gtgtttcgcg caaaaaagaa
     5281 aaacattgcg aaacaatttg cgagcgaggc tgggaaccag aacagaagaa atccttgaag
     5341 cagcgaactt accctcagat gcacgagcgg aacagctgac aatctctcaa ttcgcagtct
     5401 tatttccatt ggccctataa aatgagcgtc caagtctctg ggtttctcct cttttccctg
     5461 tgtcctctgt cttaaatatt ttcaagcttt tgaattttat ttccccggtt cctgcaacac
     5521 gcccagcaat cgatccggat aatccgtaat gattccatcc acgccaatcg caatcaggcg
     5581 ctgcatctgc tcgacttcat taatcgtcca cgcaaccact tgcaatcccg cgtggtgagc
     5641 cttctcaatc acttcggatg tgatcagatc tccgcgagcc acaagctgcc gcgctccagc
     5701 ccgtaccgtg cgctccacca aatcgctcgc gggatgctcg cataaaaatc cggtcatcat
     5761 tgtggagtcc agccggtgaa ccgtgtccag cgcggtggga tcgaacgaca gaatcaccac
     5821 gcgagcgccc gcattgtaat cccgcaaagt cgcaaccacg acgtgctcaa ttccccactg
     5881 ttggcccgcc ttaatttcga gataaaaaat aacgtcgttt tgcttcgcga aatccagaat
     5941 ttcctcaagc gtgggcactt tctccccggc ataggacttt gctccgcgat cgccgaacca
     6001 cgcgcctgca tcgagcaccc ggatctgatt gagcggcaac tgatgaacat ctcccttgcc
     6061 agtggtcgtg cgatcgagcg tgaagtcatg catggcaatc acgcgcgcat ctcgtgagag
     6121 ttgcaagtcg gtctcaataa aacgcgcgcc taattccacg gcccggcgga acgccaccat
     6181 tgtattttca ggcgcatgcc cggaagcgcc gcgatgcgcg atggtccaga tggatttgct
     6241 ttccatttcc tctttaccct gagcatgaag tgcgaagggc ttatcgttgc caaaaactca
     6301 aagtttaacc accgttttca tcgtaccaga atgcgctgca ccgactcata acgttccgaa
     6361 ccatatgtgc tcaggattct ttttgacgaa tttcggcatc gaaactaatt gcaaaggaaa
     6421 gatcgaggga cgaccgcaag gccgcggaaa agagggcttc catgggaaac cgtgaaagtc
     6481 ttctaaggta tttgtttgcg attgttttga tcggaagctt gagccttgtg tagggcgcgc
     6541 ttcgagcaat ggccgggagc acgacaatgt atacccactg gatattcaag acaattaccg
     6601 aatagcacta ggcgtcttgg tattgatttg tttggggaat gggctacttc ttcgaccggt
     6661 ctttcaacaa acccttcaac tcggacatga attccttcac gtccttgaaa tccagataga
     6721 cggatgcgaa tcggacgtag gcaactttat ccagcttctt gatgcggttc atcagcaact
     6781 cgccgacttc gctcgtcttg cgttcgcgct cgggcgattc gtgcacgaag cgctccacct
     6841 cgtccaccag cgcttccagc ttcgccgtgg ccacaggccg tttttcacac gatttgagca
     6901 gtccctgcaa aattttttga cgatcaaatc gttcgcggcg gccatctttc ttgatgacca
     6961 tgtacggaat ttcgtcgatc cgctcgtagg tcgtgtagcg tcgcccgcac ttcaggcatt
     7021 cgcggcggcg gcgaatcagg tcgcctgcgc gaccttcgcg agagtcaatg accttgtctt
     7081 ccattaccga acaaaaagga catttcacta cggcttttct cccagcggtt tttgtttcgc
     7141 cgcgctcgaa gaagggctgg agccattctg ctgtgtcacc ccaatatgtt tgcccgattc
     7201 ttccgcttcc gcttcggccc ttgccaactg ccgcaattca ccgacggaca ttccttcgtg
     7261 aatcagtagc ggaatgccgg ccagtgtacc cccggcaaat gtaatgatcc agaggactac
     7321 ggccatggcc gcggcaggtt cctgctccac gccaaaaatt ttagtcagtc cgataaaact
     7381 ggcgatttgc gctcccccgc caactcccgg aagctgcaag actgatccaa ccaacgtgac
     7441 cgccagcaga agcatcgcgc cggaaaaatt catttccgag tgaaggaagg aagcaccaaa
     7501 cgatttcgcg acgccaagat aaatcagagt cgcaagcacc caatgcgcca gcgtataaaa
     7561 aatggccgca atcagatcgg aagtcgtgcg aatcgcctgc aacccttcgc tgattcccgt
     7621 aatggccgaa gtgaagcggc tgcggaagcc cccagcgtcg cgccagcgta tcaggatgcg
     7681 atcgagaatc gctgctccgt gcatgcgata aacgacaagt aggataatca gagccgtcat
     7741 tgcgccaaac agcaaccaac cgccgtgccg cgcgccttcc acccaattca tatccgcgcc
     7801 cgcgtcggac aaccgactcg ggaagaccag aagcgcgaga caagccaagg cggcagcggc
     7861 tgcgaaatcg caaaaccgtt ccaaaaagaa aattccgaat aagctggcca ccgggagccg
     7921 ttcttttcgc gcgagcagca atggccggac gggttctccg gcgcgcccga ggacaaagat
     7981 cgccgcgaag cccattagtg ttccggagta ggtattcagg aaagtcgttg gccctacgta
     8041 tttacagaac cgttgccagc gaacggccct gattgcatag cacccataaa ttccgatgag
     8101 gcacagcagt aatagaagaa catttgcctg acccacactg gcagcaaatt tgttccacgt
     8161 aaaatcggct aaatgcactt tatgacgcga ttggtaggca actaccacca gtcccgccaa
     8221 aatgatgagg gcaatcagta tccgacgaag tcttccacgc atccgcacgc gaggttaacc
     8281 gctgcggtaa ctaggtgtca atacgagggc cagccagcac gatccgaata gttagtatgt
     8341 cctttactac caggtgaatg gagaattgta aatgtttggc gcaatactga aaaacctatg
     8401 tgcctttcgt ggcgcaggcg tccctccggc ggtttttgtt atcggatcga gggccgaaaa
     8461 gctgccggcg ggacgtcgcg ccacgaaaac agagctctaa acatttcggc tatcgctcaa
     8521 ccatgcgttc caaagtactg aaaaattccg gatacgatac tccggcgcat tcggcatctc
     8581 gaatcacggt ttcgccctcg gcgcccagcg ctgccacggc aaacgccatg gccatgcgat
     8641 gatcgccatg cggctcgatc tccgctccgt gcaaccgccc agcggaccac cccgccacgc
     8701 gcaagccgtc aggaagttcc tccacctgcg cgcccatgcg gcgaagattt tccgccagca
     8761 cggcgatgcg gtcgctttcc ttcacgcgaa gctcggctgc atttcgaatc tcaattccct
     8821 gctcggtgta cgggcccaga acagctatcg ccgggagttc atcgatcaat tgcgcgatta
     8881 cgtcgccttc aatcgagccg cctttcagcg attcatgccg gatcgatacg tcgcctacaa
     8941 gctcgccgtg ctcgctacgg atcgaaacca ggctgatcgg cgcgcccatc gaccctagaa
     9001 cgtcgagaac agccgatcgt gtgggattca gccccacatt gtgaatcacg aggttggatt
     9061 cgggcagcac caaggccgca gccaaaaaga acactgctga cgagaggtcg ccagggaccg
     9121 ctagcgagcg tcctttcaaa cgcgggcgtc catgaattcg aatggtgtgg ccctcgtggt
     9181 caagtcgcgc gccgaattcg gcgagcgcca attcagtatg gtcgcgcgtt cgaaccggct
     9241 ccgtcactga cgtaagaccc tcagcgaaca ttcccgccaa gagaaccgcg gatttcacct
     9301 gtgcgctcgg aacgggcaga gaatattcaa ttgcaacaag tttcgtgccg tcaatcttga
     9361 gtggaggaaa gccgccttcg cgggagtgaa ttcgcgcgcc catctgggtg agcgggtcca
     9421 tgatgcggcg catgggacgc cgccgcaggg aggcgtctcc gtcaatcgtc gacttaaagt
     9481 cctgccccgc aagtatcccc gcgagcatgc gcatcgtcga gcctgaattg cccgcgtcca
     9541 gagcgcggcg cgtttttcgc agcccatcca agcccgtgcc tgcgatgcgg acccgatcgc
     9601 cctcaatatc gattttgact ccgagctgcg aaaaacagtc gagcgtgctg cggcaatcgg
     9661 ccgcacccga aaaatgggtg atctcgctag ggccttcagc aagagacgcg agtatcgcgt
     9721 atcgatggga aatggatttg tcgccgggaa cttcaagtga gccgctaatt gtgcgagcag
     9781 gacgaatgat ttgtttggac acgccagaat tctagcatgc acataaaaca agatgtagcg
     9841 ctcgccttcg gacgggcaag tttttagctg cgggagcgtc accataaacc acgcgaaccg
     9901 cgccatgtcc caggaaaaaa ccggattgcc aacaaggcta gttaacgggg tatgatctag
     9961 caaatgcact tctgaggagg catgaaaatg aaatatttta ttgggttagc actggccgcg
    10021 ctgctcgcca tcgcgccggc attcgcacag gcgcctaaag gatggaagat gcacatggat
    10081 cacagcacgg atgcgtctga tccggatgca gccggcgcca ataaattcac cgtcagtgga
    10141 tcggggttcc atgctgtaaa tcccatggcc gccgtttact ggaatcccga aaacacgatg
    10201 acgggaaatt acacgcttaa agggacattc aagctgatca agtccagtgg ccacgatgag
    10261 tattacggtc ttgtcttcgg cggcagcggc ctcgaaggtg ccgctcaaag ctacatctat
    10321 ttcatgatca cggacgacgg cacgtacctc atcaagcgcc gcgacggaag ctccacgcag
    10381 ggaatttcgc aaaaaacgcc gaatgccgct gtaaaaaagc cggacgcgag cggcacagcc
    10441 accaataacc tggaagtccg cgtgaaagca gacaaaatcg atttcgccat caacggcacg
    10501 gtcgtaactt cgctcccaaa aaccggagcc gccgcaaaaa ccgatggcat ctacggaatc
    10561 cgaatcaatc accaactcga cgttcagatc gacgggttcg cagcttcgaa gatgtaattt
    10621 gtaagggcag acatttttga gtgccttgcg aacatgtctg ggaaatctat ttgtgccgta
    10681 caggcaaaaa ggcgcccgcc cgaaaagaag agcgggcgct acaaaagcaa tgcatcgcac
    10741 taaaacggag atcgttgaca ggccactcac gctatgttct aaatggccca tgccgtcgga
    10801 tcgtgaaaaa gaagcaaaac aacgtttcca ggatgcgttt gaggcgcaga tggcaggaga
    10861 actggatcgc gctgtcacac tttatcttag atcgctcgcg ctatttccta cagccgaggc
    10921 tcacactttc ctcggctgga cgtaccactt ccagggaaaa attgagcaag ctattgcgga
    10981 atgcaagcgc gctattaaaa tcgatccgga tttcggcaat ccgtacaacg acatcggcgt
    11041 ctacctgatc gacctcgacc gccacgacga agctattccg tggctcgagc aggccatcat
    11101 agcgaagcgc tatgaaccgc gtcacttccc ttattacaat ctcggacgca tctatctcgc
    11161 caaaggcctt ctcaaccgtg cctgcgaact tttccagcaa tcgctgaatg tcgaaccaag
    11221 gtattcgctt gcccgcgtcg cactcgaaaa tgcccgtcgc atggtgaatt agaaacgatc
    11281 tggcactgtt gacatccccg gttccgctgc cgatctgcag taggccgcct cttcagaggc
    11341 ggggattttt ggcgctccag catctaaatc ctcgcctctg aagaggcgag ctacaattcc
    11401 ggcgccacaa agcccttagt tttgctatac actacgcgca gaaattctgc tgccgcaacg
    11461 gaaaggcata tcaaaaatga aaatgctgct tgtggcgatg atattttttc aggccatgca
    11521 gggcgctccg ttggtcgaca atgaattcgt ccgcgtaatt aaaaatagcg cgccatgcgc
    11581 ccctgctgcg ccttcctgcg gagatcgtat cgtggtggct ctcggcccca tcgaaataaa
    11641 tggacaaaag ctcgaacgcg gcgaagtcaa aggattcaaa gccggcgagc gttattctcc
    11701 tccgaaagat ggaaattttc tcgaagtgtc catcaaaccg acgcatccga aagtaatgtc
    11761 cccaggcgcc gggacacccg cagcaccgca caataaagtt ttgtacgacg gaaaagacat
    11821 caccgtattt gaagaaaaaa tgcaaccaga cgagtacgat ccgccgcaca gccacaatgt
    11881 acgcctggct attttcctca atgacacgat ggtcgaacaa tggaccgaag gaaaagatga
    11941 aacgcgcgaa ctgattcctg acgtcgtaac ctggcggcca gcggtggtgc atgcttccaa
    12001 ggacgtaggc aaagttccga tacataatat tttgatcgaa ttcaaaccat agggcggatc
    12061 gaatgaaacg cagcacaaat cgaattctta caactcatgt gggcagcctg attcgcccgc
    12121 tccgtcttct cgaacttgtg cgcacgcggg aaagcggcaa tgcggccgat gtgcatgcgt
    12181 atgagcaatg cttaaaagaa tccgtagccg aagttgtaaa acagcaggcc catgcgggca
    12241 tcgacatcgt caatgacggc gaatttggca agtccaccag ttgggcgctt tacgctttga
    12301 agcgcgtcag cggcttcgag atgcgccccg tcaaacccgg cgcaaatcca ttcgcgcgcg
    12361 gcgcggatag agagcgcttc aaggaatttt acgcggaact cgagagcggc aacgatcgca
    12421 catggtccaa cgtaacaaag caagaggccg tctgcgtcgc tcccgttaaa tacactagcc
    12481 tcgccgaact tcaggatgac attgacaatc tgaaagccgc gctgcaaggc gcaaaagtcg
    12541 aagaagcatt tcttcccgta gccgcgccct ccagtgcgat tcccgatcgc aagaacgaat
    12601 attataagaa cgaagaagaa ctcgtggtcg cgctggccga agcgctgaga acggaatatc
    12661 gcgcgatcgt ggacgccgga ttcctgctgc aagtggacga tgcccgcgcc gcggtcacct
    12721 atgaccggat ggttccgccc gcaagttttg aagattatta caaatgggtg ggctggcacg
    12781 tcgaagtttt gaatcacgcg ctcgaaggca tccccgaaga tcgcattcgc taccacgttt
    12841 gctggggaag ctggccggga ccgcacacga cggatgttcc gctgcgaaaa attgtcaatt
    12901 taattttaaa agtccgcgcg ggggcgtatc tgatcgaggc cgccaatcct cgccacgagc
    12961 atgaatggcg cgtctggaaa gacgtgaagc tgcccgaagg aaaaattctg gcaccgggtg
    13021 tcgtcagtca cggcacgaat gtggtcgaac acccggaact cgtcgccgag cgcatcacgc
    13081 gcttcgccaa cctcgtgggc cgcgaaaatg tcatcgccag cacggactgc ggtttcgcgc
    13141 aggagcagtt caaccgccgc gttcatccca gcatcatgtg ggccaagctg gaggcaatgg
    13201 ccgaaggtgc gcgcatcgcc agcgatgaac tatggggacg tgcagcagca gagcgcgcgt
    13261 gatgagctct ccggtgagca tgacataagt cacgtacgcg ggggaccccc tgggaataca
    13321 catatagatg gagccggaag agcgcgggac ttccttcgga tcatgccaag gttccgctga
    13381 accggcacga ggagacggga aacaaatgaa aatcctgatt gcaattgacg gttctgaatt
    13441 ctctcaagcg gctttgcgca gcgtgattgc aaggccatgg aacgcgggca cggaagtaaa
    13501 agtcttgcac gtggtcgagc cgccttccct tttgattggg cgcgaaatgg gcccgtatga
    13561 ccctgagttt gaaacggttt ggaaggcggt gagggaacag gccaaggatc tggttgaaaa
    13621 ggctacggaa aaactgcgcg ctgctggatt ccaggtttcg acccagttgg tggaaggcaa
    13681 tccaaaatcg caaattatcg acgcggctaa tgaatggcac gcagacatga ttattctcgg
    13741 atcgcacggg tggtccgggt tgaaccggtt tctgatgggc agtgtttcgg agggcgtagt
    13801 gcggcacgca cactgctccg tggaaattat tcggactgca tcgtaaaggt actgcaaaag
    13861 atgttgcagg gacgcaggac catgaaatca attcggacga gctaccgtgc gtggcggcgt
    13921 tgtctgatct ttctttagct ttgaattttg cacccctgcg attgcccagc gaaaacttct
    13981 atcctcgcaa tggtaatttt cggcgacgtt cgcatcaccc gagccattga aaattggcgt
    14041 ttgcgggtac gggcacaacg ccagagtgcg atccactgcg ccattggtta aatgagacgc
    14101 gataattttt tcgggcgcga cgccgtcttc cacccagcga tcgagggcca ttacaatatc
    14161 atgctgcgcg tcgagttgcg gcggaagccc ccggttcgcg ccgccgaaaa cgttcgggcc
    14221 gggaccgttg ccgcaatgat gcatccccgg aaccatgaac aatctcataa aatccatcac
    14281 tgcgtaggca ttcggctcgc cctgcacctc cgcatactct tttcccatcg tttcgattac
    14341 gtcttcgtaa tattcgattg agcgcagcgg cgtaatcgaa tggtccgccc agccatgata
    14401 cataagcaat ttcccgccat gcgctttgaa ctcagagagg tcgggattgg cattatcctg
    14461 cgcgaccgca agcggcacga gagccgcaag cgacgcgtca aatttgaatt gctttaccag
    14521 atcaaagtta gcgccgaaca caaactgcct catgaaaata tcggaaagcg cccacgtcga
    14581 tgccggcttc gcagcgtccg ttcccgtgcc cgtcagaaaa acctggtaat cgtcttcgtg
    14641 cccaggaaca aacccgggat ggagttcctg gccggtcgaa cttaccggac cttctaaaat
    14701 cttttgaagc gcccgcgcct ggcccgccgt caggcagttg gggccatcgc ccgacgggca
    14761 gaccatcatt ttcggttcga acttgcagcg ggcgggagtc atgagcaggc cgttaattgc
    14821 gccgccgctt tcgtggcacg cgctcatcgt agctttttcg atcaccggaa ttttggcggg
    14881 cggaatcgga gcgggagcca aagcctgcgc gttccacgcg tactgcatca tcaatttcgt
    14941 ccggtcaatc accggatcgc cggaaatggc tgcatcgaaa tcatcgggat atctttgaat
    15001 ttcagcgagg gccatctttc caccgttcga gcaacctaca aaatatgcgc gccgctcatg
    15061 ttgactgtaa aaggcgcgta gaatttgttt tccgatcgat gtaaccacgt gcgtggcgag
    15121 ataggcataa ttattttgcg cgtccagatt attcagcgcc cagtttacgt ctttcgcctc
    15181 gtgcccggtg tccgaaccgg ccgccgcata gccctcggcc agggcttgcg tgagtttggg
    15241 aatcacgccg ttatatccgc cgccgcccgc gacaaaatat tttccattcc atttcgtcat
    15301 gggcaatgaa acttcaaaaa gaatcgtgga ctgcggtagg ttatgaatgt ttccaagaac
    15361 cttgcaatat tccgggatca tccccgatgc cggaataacg gctgcggagg aaatcgtcgt
    15421 gtctttcaat tggaggctgg tgagattggc gcaatcggct gcagcagttg ggatgatgtt
    15481 gatcgcaagc gcacaaaata gaaataatgc gtagcgtaat agcgtggacg aggcaagccc
    15541 gcccgcaggt accgtggtag acgcggcacc cgcaaaatcc gtgcgcaacc gatcgcggcg
    15601 cgagaatata tgtttgacca taattttctc cttacgcaaa cgcgaacggc agaggtcttg
    15661 tacccagcac gcggtttact caaatttcta tctgaccttg aatcgagcga taatttatac
    15721 ccggctgctt ccgtctgcaa tgcaacagaa tcggcagcac cggccgagtt agaattgcgg
    15781 ggcctgtgta aaataatcga gagaagccat caatgtatcg aattgtcaaa ggcgacttga
    15841 gcgacccgcg tgtaatcgaa ctcctgcaaa cgcatgtgac acgggcgagg gcggaaacag
    15901 cacgcggcag cgcacacgcc ttggatgtaa ccgcactgcg aacgccggat atcagtcttt
    15961 ggacaatttg ggacgatgag tcgcttttag gcgctggcgc gctcaagcgg ctctctgccg
    16021 accatggtga ggtcaaatcg atgtacaccg tagagtccgc gcgcggaaaa ggggtcggaa
    16081 gcgcgatgct gcggcatatc attgcgaccg cgcgagcaag cggcatgtcg cgcctcagcc
    16141 ttgaaacggg cagttgggat tattttcaac ccgcacggga attttataag cggcacggtt
    16201 ttcaggaatg cccgccgttc ggcgattacc tactcgactc caatagtgtc tttatgtcga
    16261 tggacctggg agaggccgaa tgaacagcat gaaacttttt ctttttggaa ccagccagaa
    16321 gcttctcagt cccaggcatg aaatttttaa atttttctgc gatggcttta acttggagag
    16381 gatcatgatt cccactgaaa catttcttgc agcgcgccat aaaagtgcga caatgcagcc
    16441 gttccactca cacaataagg gaggcgccca tgagaatcgc acgccacatg cctttcgtca
    16501 ttgccgctat ggccatgatg ctactggcaa gcgctgcaca agcggccacg aaatcgcaat
    16561 ggattgacta caagcagggc aacaccgctc tgtccggctt cctcgtgtat gacgatgctg
    16621 tgccaggtcg ccgccccggc gtgttgatga tccatgaccg ttcgggtttc tcggccggca
    16681 ctctcgcgga cgccgagatg atttccaaac ttggctacgt ggtttttgcc gaggacattt
    16741 tcggcaaggg cgtcgtcccc aagaccgttc ccgaaatgat ggacacgatt gcgatctacg
    16801 ataaagaccg cgctctgatg cgcgcgcgag ctttggccgg gcttgaagtc ctgaaatcgc
    16861 agccaatggt cgatcccgcg aagcttgctt cggtcggcta ttgctttggc ggcacaacag
    16921 gaatggaact cattgaaacg ggtgcgccga ttctcggatt cgtctccgtg cacggagcgt
    16981 ttcgcgattt cacgccggaa ggcgccaaaa acatcaaggg ccgggtcctg attctgcacg
    17041 gtgcggaaga tccggttgcg cctatcgaag aactcaacgc cgtcatttcg caattgcgcg
    17101 gagccaaggt tgattttgag gttcacctct acagcggcgc cgcacatgcg ttcacgaggc
    17161 cgcaaaaccc atcagaagtg cgcgcagacg atgaatacaa agtggcaatg acgcgcttcc
    17221 tgaaagataa cctggcgcac taaattactc cgcggaatcg caatatcgca gagcggtgaa
    17281 tctgggttgc ggatttgcct tgttgtccgt cagggtgaga ataaaaaaac gatcgtattc
    17341 ggtgtgtccg ataggagcta cggcaggcgc gccaagtgct tgcattattt tcggcagcgt
    17401 catgtcatgc cacacgacca gaacattgga agccgtgccc aagcgtattg cttcgactaa
    17461 tcggtccggt gtgctcaatg ggattacggt tgtattctga tgcaattttt ccgcgagggg
    17521 tgcagctgtt tgctgcgtgc gctgcaggtc cgaaacaaaa atttgatcga tctgcgcctc
    17581 ggcaagcata ttcgcaaggc atttcgcgcg acgatttccc gcatcgctga gcggtgcatc
    17641 cgtatcatcg gaaaccttat ccgcgtgacg cacaaaaaaa atagtccgca cgctctgagc
    17701 gtgcggaccg tgagcgcaaa tcaacgcggc aagaagagaa attcgcccga ttcttatgag
    17761 cgatctcatg gagaacctcc ctccctggtt ctctctttcg atatggacat caagaaccat
    17821 tcaccgaaaa tgctcgcgtg ttcccgtacg taacctgtag cacgaaggaa tttgcctatt
    17881 gatccggctc atcggaccga gaggcattcc taaagcggcc ctatggccgc ttttcgcgtt
    17941 caggagacca ccggaagccc gctgaaacgg tgggttcgta gaactcccgc tgaacctggt
    18001 attgagtgct gccctggtag aagccgaaga cctcattggt caggttgagg ccatacatga
    18061 caaaggtaaa tccatgagcg agtcgaatgc tgccctgtgc atccacttga aggtgagtgt
    18121 agaagtattg atcgctcaat ggcccgttga tgccgccggg tgtgggagtg ctatgcgtca
    18181 cgccatccgc caacagcgat ccgtcctggt atgcgtagga cgaaatattg gcttgattgt
    18241 aggagaggcc aacgcgcacg gaaaatcttc ccatgtcgta cgtcgggctg atattccagg
    18301 tgttcggcgc gtcgcgaagc agcaaggggt gatcggagcg tcccggcaat ccactggcgc
    18361 gggaggtcac gtagctgtaa ttcgccgaga ttccaagccc cttgaacgga cctgggagaa
    18421 atgacagatg ctggagatat gcgaattcga ctccgttgag ccatgcactc ccggcgttaa
    18481 tcggctgagt ggcaaggaat gtctgcggcg gaatcaccgg atttggcgaa aaattgttaa
    18541 ggaagaactg attctgcacg attgggttgg tcagattctt gtaaaaataa cccaccgaaa
    18601 tcatgccaaa ggagttgagg taatgttcga ccaggaagtc aatgctatcg ccagtttccg
    18661 ctttcagatt tggatttccc agattcacag tgttttgctg aattcccggg ctcccaatcg
    18721 tggtgtatgt caccgcttgg gcaatgtcct gggggtcggg acgtgaaaga ccgcgggagt
    18781 aggagagacg gaagttggtg ctaggggtaa ccgcataccg aatcgctgcg ctgggcagaa
    18841 tggaaaggta agacccgttg gccttgaagg agagcgtgtt ggcaacttgg tcaaagctga
    18901 ccgtcgaaag attcgttcct tcaaagcgaa tgccgccgac cacacgcaac ttgctggtta
    18961 aatctaccgt attcattatg tatcctgcgg aaactttttc cacgagatca tagttcgacg
    19021 ggtcgacacc ctggctgcta gctatagtaa attcactggg gttggcattg gcgaagttaa
    19081 gaatgtcttg gaacgcggga ttcggaccta gtttgtaggc gccgccgtag taattgttgt
    19141 ttgtcaggtc atttggaaac tgggaaaccg gaatggagac gccagaattc ggggtcgtcg
    19201 taatcgagta ggcatcgtca aatttgtggg cgttgcgaaa cttgccgcca aattcaaacg
    19261 tggccggatg agaaccgatg tggtagcgct tcgccatctc cccatcgaat tgcagattga
    19321 gctgcgcggt aagtccgtga ttaattgtga tttgattcaa ggcatatgct gaagtattga
    19381 acgcttcagt gaagcataca ggcgtaaatt gcggctcata gaaattcgtt gtgagcgaag
    19441 gggaaaattg gcaggagctg ccgggcagcg tggaaccaaa gctggccaag ccatccccct
    19501 ggttaatcag acttgcacgg cccgcagaga gattccacga aaccaaaatg gtattgaagt
    19561 cgtgtcttcc accgattaaa acactgccga cggaaatatc tggccgccga atctgggtgc
    19621 cgaagcttgg cgttcccccg ttaccgttaa gcgtggtaac tcccggcgtg ttgtcgttaa
    19681 tgccgtagtc aaagcgatcg ccgtagttct tgaaatttga aaatagtccg tgcgcataga
    19741 gattggatcc ttccgtaagc ctgtagtcca agctcccggc cagtccccac cggctgcgat
    19801 agtagcgata ttcacgcatg tctgcggtat cgaaaaagcg ctcggtgcct ccgccgggaa
    19861 gagtggcgat gtccggtgtg ggctccacat catcaatccc gcggcctgtc caatcgtagc
    19921 tcccgccgat caatgccccg aagcgtttct ggctgccaaa tcgctgacca atggtgccgg
    19981 tcgtttcaac ctgtccgcgc cctcccagga ttggcataaa tccgcccatg ccgctgaggt
    20041 tgatcgtggg ctgatcggta gccgatttgg ttatgaggtt cacggagccg ccgattccgt
    20101 ctccatccat gttcggcaga agcgttttgt tgatttccac ggactcgacg atgtcggcgg
    20161 ggatggaatc aaatttgatc tggcgaactc ctgactcagg cgacgggaca tccattccat
    20221 cgatcatggt gttggtcagg cgcggttcga gaccgcgaac ctggacgtac ttgccttcgc
    20281 cttcgtcgcg ttccagcgtg acgctcggta accggcccaa ggcgtccgcc atgttggcat
    20341 tgggcaagct gcggatgacc tccgcaggca aaacttggat gatattatcg gcagtgcgct
    20401 cgacattgat tgattccgcc tcgccttgca ctcgatcggc agtaaccacc atttggtcgc
    20461 tcacggcggc gacattcagt actgcgtccg tgctcgctcc ctgacctgca atgacctgca
    20521 cggctttcgt gaaattggca agtccgacgt aggaaacggt gacggtatat tggcccgccg
    20581 cgacgtctgt gattctaaat tgaccttgct catctgaaac agttttcgct cccgtcggat
    20641 cgagttccac gagcgcgcct ttgagcgcgc ccccgctgga atctttgacg gttccgctga
    20701 tggagccctt tccgtcctga gctgatacag ggcgcaatcc ggacacatga aataaaaaac
    20761 tagatagtaa gaggaaataa ataccccttc gcatgattca atccctccat ttgtagttat
    20821 tgctagccgt ttccacagga tccgaaattt acaagaccgc aactcggtaa caagcttccc
    20881 cggatgctta gcgctttatt tttccaaagt aacgagctgg ttgaggaact tgctgggccc
    20941 cgccccaagc tccgaatcaa aggggtcaaa aacgagcttt aaagtagtgt gaaaatatga
    21001 acgatgtgtt acgagcacgt aaaaaccaag tgaaggaatt cttacttcgg tggaaaaaaa
    21061 caaacggcaa agggaggata ttccagagct gattataatt ttccccgctc gctcagtcgc
    21121 gagtcggcac taatctcagg gtaattaatc tctttcgcga ttgttacaat tcgtgatgga
    21181 ctttttttta ctcaagatct tcccctctcc cactttttct ctctgtttta gttttatttc
    21241 cctcccctgc ttcgaaactg gaaatattta tgtgcctgca agcaagggtc taccgcccgg
    21301 gggagttgtc gctttgttac aaccacattg ctattagatg aatattgtgt gaataattgc
    21361 ccttgaggct gcggtttggc aagttcggct ggtcgtcatg aaaaagaggc ttcggattac
    21421 ctggtttctc gccgcatgcc tgtgcatgcc gctggtttcg agcgcacaat cgcccccgcc
    21481 gcgcgccgtg gtttcggtca gagagttgag cattcccccc agagcggtcc acgaattcca
    21541 gaaaggtatc gaccgcctga aaaaagagga tgcaaacggg agtctgcctc acttcgaacg
    21601 ggcagtcgcc gatttcggcg attactacga ggcttacttc gaaattggaa ttgcggagct
    21661 g
//
LOCUS       DASZMB010000154         6509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2363361, whole genome shotgun sequence.
ACCESSION   DASZMB010000154 DASZMB010000000
VERSION     DASZMB010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6509
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2363361"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(258..330)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            881..1282
                     /gene="queF"
                     /locus_tag="VG272_07000"
     CDS             881..1282
                     /gene="queF"
                     /locus_tag="VG272_07000"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HWF13465.1"
                     /translation="MADYTSEHARAGISAKLPQLDTWPNQFPGYRIVTRYPEYTSVCP
                     KTGLPDYGTITIEYEPKKACLELKALKMYLLAYRDLGIFYENAVNRMLRDIVDAIHPV
                     WCEVRGEFTPRGGLTTTVEARWPRNTRSRKS"
     gene            1431..2291
                     /locus_tag="VG272_07005"
     CDS             1431..2291
                     /locus_tag="VG272_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13466.1"
                     /translation="MSISPNVPEPGNHEQNFYEAAPTPRWIMVVFAVAFLLIGYFVYA
                     GNTAKSKLENDLAQANSKEAILSVQIDQTNSRVADLKGQLEVTTQKLGFTQAELAQAR
                     ALAQNIRESQKESDEKLTTQIGQVKQDTDAKIGQVSTDLTGAKGDITATKQDLEATKE
                     KLTSTVGDLGVQSGLIARNQEELDQLKRSGERNIFELHLAKSKTPTHVGPIQVTLTKT
                     DPKRFKYTLMVVADDKTIEKKDKTADEPVQFYIKGATRPYEIVVFDVTKDKVNGYLST
                     PKDVGSARAQ"
     gene            complement(2448..4904)
                     /locus_tag="VG272_07010"
     CDS             complement(2448..4904)
                     /locus_tag="VG272_07010"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWF13467.1"
                     /translation="MATEPMTLPVGLPAEKLRWRCDPATIPFATTAEAEPVKELLGQE
                     RAFRALKMGVELNAPGFNIFVCGLAGTSRGGTITHLIDQIHPPSKASLDRCYVNNFKS
                     PDRPRLIALPRGQANGFKKDMQSGIEFLRRRIPQVFEGEPFQRQKGRIVDRFTVREKE
                     LMDDFTHRIAREQFALGHMQVGAVALPEIFPVLEGQMVPIEDIPKMVHEGKLETIVAE
                     DLERKYEQFRQEFTVVYRKTLTLSRELASELSYLEQEAASVLVDGFIEELKEKYLGAT
                     VAEYLEEVRHQILDNLDPFKEREGEEEPGGVPPPDGGPRSASTERDPFRVYGVNVLLA
                     HTDEDPSSVIFETTPTYTNLFGTIQRSYDSRGGWNSDFMDLRPGSILRADGGFLVMYA
                     LDALTEAGVWRTLKRMLNHAKLEIQPLEMFFPFGSSALKPEPIDIDVKLILIGDRHMY
                     ELLYNFEEDFRKIFKVRVEFDEEMVMNDAVIRHYGDRLRQLSEREHIGPFDRTAVASM
                     IEYGVRLAGRRNKVTARFPELADLGREAAYIARQEGVELVNGAHVRRAREARIERHNL
                     TETKIREMIEEGTLMIETGGTRVGQINGLSVLEIGGYAFGKPVRITASASLGKTGLIN
                     VERESNLSGSFHDKGMHIISGYLRTKFAQDKPLSLAASICFEQSYSGVDGDSASSTEI
                     YALLSALSGLPIRQDIAVTGSVSQQGDIQPIGGVNQKIEGFFDVCRAKTGLTGHQGVM
                     IPSENIEDLMLRDDVIEAVAEGKFHILPVTSLEEGIEILTGVKAGRRDAKGVFEPNTV
                     YAQVDRRLHEMATTMKDFQS"
     gene            complement(5054..6118)
                     /locus_tag="VG272_07015"
     CDS             complement(5054..6118)
                     /locus_tag="VG272_07015"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWF13468.1"
                     /translation="MAENTDYRFNNFLFYGVILCLTYLVFRVFEPFLIPLGWAAVFAV
                     LFHSLNKRFERKWGSTQAAGLITLGVTLILIIPVLLIAVMFVREGISAAGDIQRAMGT
                     GHYGWFGKAWAWIGSKIAAQGIRLDLRGLVREEASNAGGYMAAKLGGVIRNIVVFLFE
                     LLVTLFALFYFIKDGDAILTGLRNFLPFEETMKERMLAEARELIFASVTTSVVIAVVQ
                     GLICGVAFAIVGLGSPVFWGVVMAFLSLLPVVGAWPVWVPATIWLFSSGHAGRAVILV
                     IICGAVGGMVDNVLRPVLLGGRASLNGLLVFISVLGGIAVFGVLGVILGPIVVATAVG
                     ILDVYSGVDEADRVPNVPAV"
     gene            6276..6434
                     /locus_tag="VG272_07020"
     CDS             6276..6434
                     /locus_tag="VG272_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13469.1"
                     /translation="MNDTAACRHLRTKLIAKDSDAEYVECLDCGAIIESSEMPEPSAG
                     FNESLSDA"
BASE COUNT         1677 a         1819 c         1606 g         1407 t
ORIGIN      
        1 cccattcgat atgtgatgta ggcccccagc cagaatcgtg caatcgaccc gaggaatccc
       61 cccagcccca ccatccagta ttttgtcatc cacgtcctcg ccagactttt ctttgcgaca
      121 tcctcacgga cttcccgcaa attgcaagaa gtttagacgc gcaaatatgc aacgagaaaa
      181 atatcggcac atcctgcgct tcatgaatgc tgacttgatt gactggttca ttgccgggac
      241 atgactccgc gacaaggaac ttgtcaggag tcatcatctg tccggccaaa ggacagcggt
      301 tataaggcga actccatcgc ctttggcaat catacggttt aagtaatgga agtcaaccgc
      361 gtgctttcgg aatgttccgg ttgaattaca tctttattga tgaacagcga ttgactcttg
      421 ttttagtgcc ggcggctgtt ttgtcggtat gatccggcgg taaatccgaa aagagaaaaa
      481 acgattccgg cagggcgcgc agcgataggc aggagcgcga aacaatcgat agacccagga
      541 aagcatgccc attccatgct cacttgaaat acgctgcacg tccaaattgc cgcacatgcc
      601 gcaatgaacg taccagacat atccaatcgg agcggcccat gcataaaacc gcgaatcgca
      661 gaccctgcac ctccaagggc gcagtcttac cacaccaacg gaatagtctt ttacgctgcg
      721 cctgcgggac cggcgcggac tgtcactgcg gcaaacggga caaagcaagc tcttatgacc
      781 cctgcaattc tgcgaaaaga ataatagaag gcttattcaa ttagcaagtt cgatctgtgg
      841 aaggttgcgc atgcatgtac aatgacatga gagaggcaaa atggccgatt acacttccga
      901 acatgcgcgg gcaggaatta gcgcaaaact tccgcaactg gatacatggc ccaatcaatt
      961 tccgggatac cgaatcgtaa cgcgctatcc cgaatatact tcggtatgcc ccaaaaccgg
     1021 attaccggat tacggaacga taacgattga gtatgaaccg aaaaaagcgt gtctcgaact
     1081 caaggccctg aaaatgtatc ttctggcgta ccgcgacctc ggtatttttt atgaaaatgc
     1141 cgtcaatcga atgctgcggg atatcgtgga tgcgattcat cccgtgtggt gcgaagtgcg
     1201 cggcgagttc acgccgcgag gcggattgac caccacggtc gaagcacgtt ggccaagaaa
     1261 cactcgatcc cgcaaatcgt agacaaccaa tatcggctca gtaataatct gacgtgcgta
     1321 ggtgatggtt acgtaataac attcactttg acgttttttg atgcggtagg ttttaaagct
     1381 cgcgagaata ctttggacgc gcgcgtaaat ccagccgcga aggagcgaag atgagcattt
     1441 ccccgaatgt tcccgagccg ggaaaccatg aacagaattt ctatgaagcg gcgcccacac
     1501 cgcgttggat catggtcgtg ttcgccgtcg cttttttact gatcggttat ttcgtgtatg
     1561 ccggaaatac cgcgaagtcg aaactggaga atgacctcgc ccaggccaac tcaaaggaag
     1621 cgattctctc ggtgcagatc gatcaaacca attcccgcgt ggcggatctg aaaggccagc
     1681 tggaagtgac cacgcaaaag cttggcttta cgcaagccga attggcgcag gcacgcgcgc
     1741 ttgcccagaa tatacgtgaa tcccagaaag aatccgacga aaaactcacg acgcaaatcg
     1801 ggcaagtgaa gcaggacacc gatgcaaaga ttggacaagt cagcacggac ctgaccggcg
     1861 caaagggcga tatcacagcg acgaagcagg acctcgaagc aaccaaggaa aaactcacca
     1921 gcacggtggg agatttggga gtgcaatccg gattgatcgc gcgcaatcag gaagaactcg
     1981 atcaactgaa acggtccggc gaacgcaata tatttgagtt gcatcttgca aagtcaaaga
     2041 cgcccaccca cgttggcccc attcaggtca ctctgaccaa aaccgatcca aaaagattca
     2101 agtacacgct gatggtcgtt gccgatgaca agaccattga gaaaaaagat aagaccgccg
     2161 acgagcccgt gcagttttat attaaaggcg ccacgcgacc ttacgaaatc gtcgtcttcg
     2221 atgtaaccaa agacaaagtg aacggatact tgtcgacgcc aaaagacgtg ggtagcgcac
     2281 gcgcgcaata agcgccaatc gctgcacagg cagctgaaaa gaaaaataaa tctgtcattc
     2341 cgagcgaagc gaggaatctc tctttcctct ggttaaaatc gaagagagat tcctcccttc
     2401 gctcggaatg acaggaatag tttttgattt tcacagcgag gatttaccta cgactggaag
     2461 tccttcatcg tcgtagccat ttcatggagc cgccggtcga cctgcgcgta aaccgtgttg
     2521 ggttcaaaca cgccctttgc atcgcggcgg ccggccttca cacccgtcaa gatttcaatt
     2581 ccctcttcga gcgaagtaac cggaagaata tgaaatttgc cttcagcgac ggcttcgatc
     2641 acgtcatctc gcagcatcaa gtcttcaata ttttctgaag gaatcatcac gccctgatgt
     2701 ccggtcagtc cggttttcgc ccggcacaca tcgaagaatc cttcaatctt ctgattcact
     2761 ccgccgatgg gttgaatatc gccctgctgg ctgacggaac cggttacggc aatgtcctga
     2821 cgaatgggca aaccggaaag cgcggaaagc agcgcgtaaa tttcggtgga gctggcgctg
     2881 tctccatcga cgccgctgta ggattgctca aaacaaatgc tggccgcgag cgaaagcggt
     2941 ttatcctgcg cgaatttcgt gcgcaggtat ccggaaatga tatgcatccc tttgtcatgg
     3001 aagctgccgc tgagatttga ttcccgctct acattgatca atcccgtttt tccgagcgaa
     3061 gcggatgccg tgatccgcac aggcttgccg aacgcatagc cgccgatttc cagaacgctc
     3121 aggccgttga tctggccaac gcgcgtgccg ccggtttcaa tcatcagcgt gccttcttcg
     3181 atcatttctc gaatcttcgt ttcggtgagg ttgtgccgct cgattcgcgc ttcacgcgcg
     3241 cgccggacat gcgcgccatt caccagttca actccctctt gccgcgcaat gtaagccgct
     3301 tcgcgcccca agtccgcaag ctccgggaat cgcgccgtca ctttattgcg gcgtccagcc
     3361 aggcgcacgc cgtactcgat catgctggcc acagccgtac gatcgaatgg accaatgtgt
     3421 tcccgctcgg aaagctggcg caagcggtcg ccataatgac ggatcacggc gtcgttcatc
     3481 accatctctt cgtcaaactc cacgcgcact ttgaaaattt tgcggaagtc ttcctcaaaa
     3541 ttgtagagga gttcatacat gtgccggtcg ccaatcaaaa tcaacttcac gtcaatgtcg
     3601 atcggttccg gtttgagcgc tgaggatcca aacggaaaaa acatttccag cggctgaatc
     3661 tcaagcttcg cgtgattcag catgcgcttc aacgtgcgcc acactcccgc ctcggtcagc
     3721 gcgtccagcg cgtacatcac cagaaaaccg ccatccgcgc gaaggataga acccggacgc
     3781 aagtccatga aatccgaatt ccagccgccg cgcgaatcgt aactgcgttg aattgtgcca
     3841 aacaaatttg tgtacgtcgg cgtcgtttca aaaattaccg acgatggatc ttcatccgta
     3901 tgcgcgagaa gaacgttaac gccataaaca cgaaatggat cgcgctcggt ggatgcggat
     3961 cgcggcccgc catccggtgg cggcactccg ccgggctctt cttcgccctc gcgctcctta
     4021 aatggatcga gattgtccag aatttggtgg cgaacttctt ccagatattc cgcgacggtc
     4081 gcgcctaaat atttttcctt tagttcctcg atgaagccat cgaccaacac cgatgcggct
     4141 tcttgctcga ggtagcttag ctcgctggcc aattccctgg aaagagtcag tgtcttccgg
     4201 tagacgaccg tgaattcctg gcggaactgt tcgtatttcc gctctaaatc ctcggccacg
     4261 atggtttcca gcttaccctc gtgcaccatc ttcggaatat cttcgatcgg caccatctgc
     4321 ccttcgagga caggaaaaat ttccggcagt gcgacggcgc ccacctgcat gtgtccgagc
     4381 gcaaattgct cgcgcgcgat gcggtgggtg aaatcatcca tcaattcttt ttcgcgaacc
     4441 gtgaagcggt ccacaatgcg ccctttttgt cgctggaagg gctcgccctc aaatacttgc
     4501 ggaattcgcc ggcgcagaaa ttcgatgccg ctctgcatgt ctttcttgaa tccgttggcc
     4561 tgtccgcgcg gcagcgcaat aagccttgga cgatcggggc ttttaaaatt attgacgtaa
     4621 caacgatcga gcgaagcttt tgagggaggg tgaatctggt cgatcagatg cgtgatcgtg
     4681 ccgccgcgac ttgttcctgc caggccgcac acaaaaatat tgaatccggg agcgttcaac
     4741 tcaacgccca ttttcagcgc acggaacgcg cgctcctggc caagcaattc tttcaccggt
     4801 tcggcttccg ccgtggtagc aaaaggaatg gtagcaggat cgcacctcca ccgaagtttt
     4861 tcggcaggca aaccaacggg caatgtcatg ggttcagtag ccattttaga ttcttttcgg
     4921 tctcaggcgg gttactctag cacaggcaag acggcgggcg cacactcacc ggactcaaaa
     4981 tttccacagc ctgcaaataa tctctaacgt atatttcagg aataaagatg tgcctcaaaa
     5041 aagttggggg gtattaaacc gcaggcacat ttggcactcg atcggcttca tcaaccccgg
     5101 agtacacatc cagaatccca actgcggttg ccacaacaat aggcccgagg ataactccca
     5161 atacaccaaa cacagcgatc ccgccgagaa cgctgatgaa aactaacaaa ccattcagcg
     5221 aagcgcgtcc ccccagcaag actgggcgca aaacgttatc caccatccct cccaccgctc
     5281 cacagataat caccaatatg accgcgcggc ccgcgtgtcc cgaagagaac agccaaatcg
     5341 ttgccggaac ccagaccggc catgctccta caaccggcag cagcgacaag aatgccataa
     5401 cgactcccca gaagacgggc gaccccaatc ccacgatggc aaatgcgacg ccgcaaatca
     5461 gtccttgcac aaccgcaatc accacgctgg tcgtgacgct ggcaaaaatc aattcacgcg
     5521 cttccgccag catgcgttcc ttcatggttt cctcgaacgg caagaaattg cgaagcccgg
     5581 tgagaattgc atcgccgtcc ttaatgaaat agaacaatgc gaataacgtg acgagtaatt
     5641 caaataggaa tacgacaata ttacggatta cgcctccaag tttcgcggcc atatatccac
     5701 ctgcattgct ggcttcttca cgcacaagcc ctcgcaggtc caagcgtatt ccttgtgccg
     5761 caattttgct gcctatccat gcccatgctt tgccaaacca tccgtagtgt ccggttccca
     5821 tggctctctg aatatcccct gccgcggaga ttccttcgcg aacaaacatc accgcaatta
     5881 acagcacagg aatgattaga atcaaggtca cgcccaatgt gatcagccca gccgcttgcg
     5941 tgctgcccca ctttcgttca aagcgcttat tcaaggagtg aaaaagcacg gcgaaaaccg
     6001 ctgcccagcc gagcgggata aggaatggct caaatacccg gaagaccagg taagtgaggc
     6061 acaaaataac gccgtagaat aaaaaattat tgaaacgata atcagtgttt tcagccacgg
     6121 cgtttccggt tctccctcaa tcgtacagac gaagccattt gaacgctcat cttataacca
     6181 agctggaaaa attggtgaac ttcatgaagg tttaggccga gccgggcggc agaagagcgt
     6241 gctatgattt cggtagatcg tttcgaggta atacgatgaa cgatactgct gcttgccgcc
     6301 atctccgcac gaaactcatt gccaaggatt ccgacgcgga atatgtcgag tgcctcgatt
     6361 gcggggctat catcgaatcc agcgaaatgc cggagccttc cgcggggttt aacgaatcgc
     6421 tttccgacgc ttaggtgccg cgcttacacg cgattcggaa aagctctaac acagaggacg
     6481 ctgggaaatt cttgaaaagg cgagaaccc
//
LOCUS       DASZMB010000155         5402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2363518, whole genome shotgun sequence.
ACCESSION   DASZMB010000155 DASZMB010000000
VERSION     DASZMB010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5402
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2363518"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..2838
                     /locus_tag="VG272_07025"
     CDS             211..2838
                     /locus_tag="VG272_07025"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWF13470.1"
                     /translation="MKTLDNSKIHREDIPIVSAAPLTTKKQESPLAEVIPVRPAFSNA
                     ETAVVFFLRQFRVLLGAARLYQRNHPRLMEILASTEQQLRIALAARTPLVLAIEPNGI
                     LLPGQESGASELLHDQRGELKSLAEELLRSGICSLLFTLPINVGELDHLAHEISLVPR
                     SSSPGDTASRKLWDNWIKSRGVVGIRINIPTERRDSLLLASLMSVVLAYENSTQHSSH
                     SKAAHSMPEASFEQIANTLRVLAKLIPPSDPEMQPSAGDIARRFHSVVSASESASVSL
                     IVYGVSNVRPREGEALGPYLERLTDELVLAFVEQEFNAGRATLPAISPLFVRLDKERG
                     QADNTGATRFGGSQHDELRVAQLCEKFWNTQPERVKATALRSRDAWCIPASVVARFLE
                     PLVETANKKESDAATREGREVLFSYCRCLESEENRARRTVAAGLAEISAQMEKLWPHP
                     SIAELGPEVVQALLKEASPGIAGLLSAAVEKLARTSLLKHEYGEFERILEPLEAAPKD
                     DEHAHISTLVGHILNDEQWLYLVDEALSGQPLSPVIPRLLKRCPDRLIDRLGLLLTAA
                     NGLDALPAMVRLIHGTGEPVLGALETRLYEARRQRVATAIHLLASADPKRLAAALPRA
                     LASWEWSLQDLAATELMKWTNPPVVAATAHAFLCTLAEAHPMVVPCMIDHLGMAHETA
                     AIPHLIQIAEGNHLILRDIFFRIKAIEALGRMRVPEAAITLLKIVRLRNGLAHSEPAA
                     LRSAAEEALALLENKASSTRARVAESTRAKPYVAHSRPRRYLRARVQPPMQAVISGTR
                     NGSGRVRVIALGGAYLEIEQPLAVGDSLQLEIRNGLRKIQASAVVRNVTAMGAGVEFV
                     HIKPRDRELLRRLITQLLK"
     gene            complement(3005..4381)
                     /gene="rmuC"
                     /locus_tag="VG272_07030"
     CDS             complement(3005..4381)
                     /gene="rmuC"
                     /locus_tag="VG272_07030"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HWF13471.1"
                     /translation="MTGTVILIAIACLGAGFAAAWAWASTSGGEVRLELEKRAAGLDG
                     TVVELKKQNDALQQDSRTLQKRIEEEQKLRATAEKDAESQRDNLAEQRRLLDDAQIKL
                     REAFQSLAGEALQSSSNQFIELAKSKFDSLQKEAEGDLDQRKIAIQAMVDPLQTALKD
                     LRSEVSRVESARQEAYGSLRSEVQLLATTNRELREETGSLVNSLKQPQIKGKWGELTL
                     RRVVELAGMSPHCDFLEQQSVDTEDGRLRPDLVIYLPGGTQIVVDVKVPLHAFFKAVS
                     AQTDADYREAMVQHASLVRDHIKGLSSKEYWKQFEPTPEFVVLFVPGESFFSAALEQD
                     RTLIEDAIDKRVVLASPTTLIALLRAIAYGWKQHLVAENAERIKDLGKELYDRVVKFA
                     EYLSEIAKGLERANKAYNNAVASFDSRLIPSARKFREMGVGSADVPQIEPVETFPRPL
                     LSTPEPEE"
     gene            complement(4591..>5402)
                     /locus_tag="VG272_07035"
     CDS             complement(4591..>5402)
                     /locus_tag="VG272_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13472.1"
                     /translation="QQPTKPHAAATPRQIILPPKVIAGAPAMLAVLDSRGRLMPKVEV
                     DISDGQKVTTDATGRALFNANNQPGSLIAKIEGPGITSATTVVSAENATSRDPARAVN
                     VSTYPRVMTQHDRFVLEGTGFRSEADSNHVSLNGDPCLVIASSPVSLVVLPGPRGLIG
                     DAQLKVAAGGSDAGLFPVSVVALEFSGPSNAVDAGSTGKLILRARGTTLPLTVEVRNG
                     SPRVIRLAQGNVQRLKTSGGDENIAPIEVNFVTGGNYFVSARLIGADIRRN"
BASE COUNT         1161 a         1506 c         1483 g         1252 t
ORIGIN      
        1 attggcatat ttggctctct aatgcccttt atttcagcag gctgaaggtg cattctgacg
       61 gcatatggcg tgctctcttt taggtacacc ccgtgtccct cgtcgcaccg ggagcaagac
      121 gatgtaccgc caaagtggct ctgaattttt tcgggaaccg acaagaaggc caagcgcggt
      181 tagaggttca aacaaggaac catctttcga atgaagaccc tcgacaattc gaagattcat
      241 cgcgaggata ttccaatcgt aagcgcggcg cctcttacta cgaagaagca ggaatcgcca
      301 ctggctgaag tcattcctgt tcgtccggca ttttccaatg ctgaaacagc cgtggttttc
      361 ttcctgcggc aattccgcgt cctcctaggc gcggcgcgtt tgtatcaacg caatcatcct
      421 cgacttatgg aaatcctggc atctaccgag cagcagctac gcattgcact tgccgctcgg
      481 actcctctag tgcttgccat tgagcccaat gggatcctgc taccgggcca ggaaagcggc
      541 gcgtcagaat tgctgcatga tcagcgcggc gaattgaaat ctctggcgga agaacttctt
      601 cgcagcggaa tttgctcctt gttgtttacg cttcccatca acgttggtga attagatcat
      661 ttagcgcatg aaatcagcct ggttccacgc agctcctcgc cgggcgatac ggcatcgcga
      721 aaattgtggg acaactggat caagagtcgt ggcgtggtcg gcatacgcat taacattccg
      781 acggagcggc gcgattccct gctcctggca agcctcatgt cggttgtgct ggcgtatgag
      841 aattccacgc agcattcttc tcattccaaa gccgcgcatt ccatgccgga agcaagcttc
      901 gaacaaattg caaacacgtt gcgcgtcctg gcaaaactga ttccaccaag tgatccggag
      961 atgcaaccct cggccggaga tattgctcgc cgttttcatt ccgttgtgtc cgcaagcgag
     1021 tccgcttccg tttccttaat cgtgtacgga gtttcgaacg tgcgacctcg cgaaggggaa
     1081 gcgcttggtc cttatctgga aagactgacg gacgaacttg ttctggcatt tgtggagcag
     1141 gagtttaatg cgggccgcgc caccctgcct gcgatcagtc cattgttcgt tcgtctggat
     1201 aaggaacgcg gccaggccga caataccgga gcaaccagat ttggcggttc gcaacatgat
     1261 gaactacgcg tggcgcagtt atgcgaaaaa ttctggaata cccagccgga gcgcgtcaaa
     1321 gcgactgcgt taagaagtcg cgacgcatgg tgcataccag ccagcgtggt ggctcgtttc
     1381 ctcgaacccc tggttgagac cgccaacaaa aaggaatctg atgcggccac acgcgaaggc
     1441 cgcgaagtac tgttttcgta ttgccgctgt ctggagtcag aggaaaatcg agcgcgacgc
     1501 accgtggccg cgggactcgc ggaaatttcc gcgcagatgg aaaaattgtg gcctcatcca
     1561 tccattgcgg aattggggcc ggaagttgtt caagcgttat tgaaagaagc ctctccagga
     1621 attgcaggtc tactttccgc agcggtggag aagttggctc gaacttccct gctgaaacat
     1681 gaatatggtg aattcgaaag aatccttgag ccgctcgagg ctgcgccaaa ggacgacgag
     1741 cacgcgcata tttcgacgct tgtcggacac attctcaacg atgagcaatg gctgtatctg
     1801 gtggatgaag ctctttccgg ccagccgctc agtccggtaa ttccacgctt gctgaagcga
     1861 tgtccggacc gcttgattga caggctggga ctgctactta cggctgcgaa tggattggat
     1921 gctcttcccg ccatggtgcg cttgattcac gggacgggag agcccgtgct cggcgcactg
     1981 gaaacaagat tgtacgaagc gcggcggcaa cgggttgcga ccgcgattca tttgctcgct
     2041 tccgccgatc cgaagcgcct cgcggccgct ttgccacggg cgctggcgag ttgggaatgg
     2101 agtttgcagg accttgccgc cacggaactc atgaaatgga cgaacccgcc tgtggtcgca
     2161 gcgacggccc acgcatttct ttgcacactc gcggaagccc acccgatggt tgtgccgtgc
     2221 atgatcgacc atctcggaat ggctcatgaa acagcggcca ttccacacct gattcaaatt
     2281 gccgagggaa atcatttgat ccttcgggat attttttttc ggatcaaggc gatcgaggcg
     2341 ttgggccgaa tgcgcgtacc ggaagccgcc ataaccctgc taaaaattgt aagattgcgg
     2401 aatggcttgg cccacagcga accagcagcg ctgcgatccg cagctgaaga agcgctggcc
     2461 ttgctggaaa ataaagcgtc ctccacgcgt gcccgcgtgg ctgaaagtac acgcgcgaaa
     2521 ccttatgtgg cgcactcgcg tccccggcgt tacctgcgtg cgcgtgtgca gccgcccatg
     2581 caagcggtga tttcaggaac acgcaacgga tcgggacgag ttcgcgttat cgccctcggc
     2641 ggggcatatc tggaaatcga gcagccgctg gcagtgggag actcgctcca gctcgaaatt
     2701 cgtaacggac ttcgcaagat tcaagcctct gccgtggtgc gtaatgtcac ggctatggga
     2761 gcgggcgttg aattcgtgca cattaagccc cgcgaccgcg aacttttgcg gcgcctcatc
     2821 acgcaactgc tcaagtaaat ttccccactc gatttttcaa atcgcatttc ttgagcgcgg
     2881 ctttatgtgg ctcgggcttc accctgcggg ggtcctgtag cgccagcgtc tcaccggcgg
     2941 ttttaagtta tcgcaatgcg acgcaaaccc gccggcggga tgccggcgct acagaccacc
     3001 tgccttattc ttctggttcg ggtgtggaaa gcaagggacg aggaaatgtt tcgacgggtt
     3061 caatctgtgg aacatccgcg ctgcctacgc ccatttctct aaattttcgg gcggaaggaa
     3121 tcaggcggct atcgaatgag gcaacggcat tgttatacgc tttattggcg cgttccaagc
     3181 cttttgctat ttctgaaaga tattcagcga atttcacaac gcggtcatac aattctttgc
     3241 ctagatcctt gatgcgctcc gcattctccg caacgagatg ctgtttccag ccataggcga
     3301 tcgctcgcag aagcgcgatg agcgttgtcg gactggcgag gacgacgcgt ttatcgatgg
     3361 cgtcctcgat caacgtacgg tcctgttcga gcgcggcgct gaaaaaagat tctcccggga
     3421 cgaataaaac cacgaattca ggagtcggct caaactgttt ccagtattcc ttgctggaca
     3481 aacctttgat gtgatcgcga accaagctgg catgctgaac catcgcttcg cggtaatccg
     3541 cgtccgtttg tgcgctgacg gctttgaaaa atgcatgcag cgggactttt acgtccacca
     3601 cgatttgggt gccgcccgga agataaatca cgaggtcggg acgcagccgg ccgtcttcgg
     3661 tgtccaccga ttgttgttcc aggaaatcgc agtgcggaga cattccggcc aattccacca
     3721 cgcgcctgag agtcagctcg ccccactttc cttttatttg cggttgcttg agcgaattga
     3781 ccaagcttcc cgtctcctcg cgcagttctc tatttgtggt ggccaggagt tgcacttcgc
     3841 tgcgcaggct gccgtaggct tcttgccttg cggattccac acgagacact tcggaacgta
     3901 agtccttcag cgccgtttgc agaggatcca ccatggcttg aatagcgatc ttgcgctggt
     3961 cgaggtcgcc ttcggcttcc ttttgcaagg aatcgaattt ggattttgcg agttcgataa
     4021 attgattgct gctggactgt agggcttctc cggcgagcga ttgaaatgct tcgcgaagct
     4081 taatttgcgc gtcgtccagc aaacgtctct gctcggcaag gttatcccgc tgcgactccg
     4141 catctttctc agccgtggcg cgcagcttct gctcttcttc gattcttttc tgcagagttc
     4201 gcgagtcctg ctgcaaggcg tcattctgtt ttttgagttc aacaacggtg ccatcgaggc
     4261 cggcggcgcg cttttcgagt tccagccgta cctcgccacc cgaagtactt gcccacgccc
     4321 aggcagctgc aaaacctgca ccgagacatg cgatagcaat cagtattacc gttcctgtca
     4381 tgaaggcgcc ttgagcgaag gatttgacct aactatatag ctgagaaaga aattctatca
     4441 cgccgaaagt agcacaggct tccgcccgcg gggtatcgta gtgcgcgcac aagaatccat
     4501 gcgctgaagc ctgtgctacc aaaaccctct gcctgaaaac gtttgccatg acgggtcaac
     4561 tacaagccaa gatttaaatt tacgcggcgt ttaatttcgg cgaatatccg ccccaatcag
     4621 cctggccgac acgaaatagt ttccgccggt cacaaaattc acctcgatcg gcgcaatatt
     4681 ctcgtcgcca ccggatgttt tgaggcgctg aacatttccc tgcgcaagtc gaatcactcg
     4741 cggcgaacca ttgcgaactt ccacggtaag cggcagagtt gtgccgcgcg ctcgcagaat
     4801 cagcttgccc gtggaacccg cgtctaccgc attgctcggg ccgctgaatt caagcgccac
     4861 cacagagact ggaaacagcc ctgcatccga tccaccggca gcgactttta attgggcatc
     4921 tccaatcaga ccgcgcgggc cgggcaaaac gaccagcgaa accggcgagg atgcaatcac
     4981 caggcacggg tctccattca gcgagacgtg attggaatcc gcttcgcttc ggaagccagt
     5041 gccttccaat acaaatctgt cgtgctgggt catcacccgt ggataggtac tgacattgac
     5101 cgctctagca ggatcacgtg acgtcgcatt ctcagccgag acaacggttg tggcggaggt
     5161 aattcccgga ccttcgattt tggcaatcag cgatccgggc tgattattcg cattaaacag
     5221 cgcgcgaccg gttgcgtccg tcgtcacttt ttgcccgtct gaaatatcca cttctacttt
     5281 cggcatcaat cggccgcgag aatcgagtac ggcaagcatc gctggggcgc cggcaattac
     5341 tttagggggc agaataattt ggcgtggagt agcggctgca tgcggttttg tcggctgttg
     5401 ag
//
LOCUS       DASZMB010000156        38824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2627672, whole genome shotgun sequence.
ACCESSION   DASZMB010000156 DASZMB010000000
VERSION     DASZMB010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 38824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38824
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2627672"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            473..1141
                     /locus_tag="VG272_07040"
     CDS             473..1141
                     /locus_tag="VG272_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13473.1"
                     /translation="MKKTFRAPAIVVIFLLSAIGMRTTAASAGQTRSATSGSWADSVR
                     NAPHFLVRLDQELNTGKNRNHEKFQVQTIEPLVASNGHIFPVGTKILGHISRIEPGGL
                     TGRARVWLTFDDVETARGTLPIVAEVSSVPGEFSVQTGKSKEGEITARTGKGAEVAEA
                     TAVGAAKGSATGVTSHNGKEAAIGAAAGGMAAFLASSGIGQEIDLPKGTKLELVLDRP
                     LYMN"
     gene            complement(1167..1595)
                     /locus_tag="VG272_07045"
     CDS             complement(1167..1595)
                     /locus_tag="VG272_07045"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HWF13474.1"
                     /translation="MKPFIVVHGIAADPVTHRVYVVDRSNHRIQVFDENGKFVDQWAD
                     DKYSSINFLYMSADRKLWAADDRNSKILEYDLEGHLLYSWGTLADWPGGLFNTHGMSV
                     DQEGNFYVAEVGNGRAQKFRPRPGANPALLVGKPVYAAWK"
     gene            1739..2317
                     /locus_tag="VG272_07050"
     CDS             1739..2317
                     /locus_tag="VG272_07050"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HWF13475.1"
                     /translation="MRIKLMQSLIAVSILSVTITAGVAAQQQATPPPPSKFKLMSSAY
                     TEGSMIPTQYSCADPNAASPALSWTNPPSAATSFAVIFHDTDAAPMKGSMDVTHWIFW
                     NVPGTSSSVAAGVKPDSSPNGIVQGVNIRKVNGFQPPCPPPGATPHHYIFEIYALDSK
                     LDLAAGSARTDLLKAMDGHVVGKATYVGIFGR"
     gene            2611..4020
                     /locus_tag="VG272_07055"
     CDS             2611..4020
                     /locus_tag="VG272_07055"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWF13476.1"
                     /translation="MADATRFETTKLSPDRVLIVEDEENARLGYEALLRRWGYEVLGV
                     GSAEDALAKFSEFAPAVLIADVELPGMNGLDLLARLGDELQNVPAIIITGKGSEERIV
                     QAIEAGAFWYIEKPLKGSVLHVLLERALGRVRDRKQVAALTRQLRESGKLGDLVGQSR
                     TMQEVMHLVEQVAPSSASILITGETGSGKEILARTIHRLSPRADRPFIAINCSAIPES
                     LMESEIFGHERGAFTGAAERRLGCFELADGGTLLLDEIGEMPAPTQAKLLRVLEDHKV
                     RRLGSKVETPVDVRVLAATNKEPEVAVAKGELRQDLFFRLNVFHIQLPPLRDHKEDIP
                     LLVEHILRDINTKHGRHVRGVNPEVLDQFAGYSWPGNVRELRNVLERAAIVTDRDLIG
                     RASLPVDFGHAPAASASDLASLRFPLGTTVEAAERELILQTLSSTNNKTRSAELLGIS
                     LKTLHNKLKEYEAAKTPSK"
     gene            4035..5966
                     /locus_tag="VG272_07060"
     CDS             4035..5966
                     /locus_tag="VG272_07060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF13477.1"
                     /translation="MQLRLKTKITLATAALVLAVVGVNSTLYVMNLSRQVIRQADERA
                     QLVSKQVFFQAQVALADAAKAGEAPDSNSSEDLREYAQKAFDESASLASSIDAQLGYS
                     PLIYEISISDVNGKVLVSSDKSMRGNPEPIRAGIEQLVSNGFVKQLRVLYSLLYGPPL
                     RAYEVTYPFQLGPPGQQIPFGAVRVAVQTGLLRASIIPALRSAALLAIISVGVSVLLA
                     GIVSSISLAPLKRITAQLDRIAKGEFDQKPLERGDEFGQVSTKISQIGMQLRGVREIF
                     SNLRENIDQVLGGLDDGLLLFSVDGRAVMVSPAVERFLSTPADQLLGRRAEDIFPPDH
                     DVRSAIKMQNGEFEPVASAEAVLRHIDGPPRRVGVSVEVIGEGDMRMGTLVKFKDLES
                     RERIGTQLQVSERLANLGRITAGVAHEVKNPLNSMRIWLENLKASLPDVEGLPLQAVR
                     VLDSEIDRLDSVVKRFLDFTRPPDMHQEEANLKGTLEEVLAVEKPELDKANIKLEAKL
                     ASNVPNVLVDKLLLKQALVNLILNAIEAMPKGGQLSVSLNRRDEMAEIEIRDTGRGIA
                     PEHRQRIFQLFFTTRAGGSGIGLASTFRTVQLHNGSIEFESEVGRGTTFRIDLPLAHQ
                     MEPALSRPRDSGAAVAGRL"
     gene            5966..6520
                     /locus_tag="VG272_07065"
     CDS             5966..6520
                     /locus_tag="VG272_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13478.1"
                     /translation="MRKFKLLALLCSLSAVMAGCASKHIVRASPPSVSVPPPDETAPM
                     PAPTTPPPGPAVTEEPPPEVESPMPPPVPGKRPAAPRRPAPTESADSTPAKPAAPQIS
                     PQLSPRDLESTKKNTTANIMTAEQNLQLSNGRQLSAAQKDLAEKIKGFLSQSHDAIRS
                     DDWVRAQNLAEKARVLSTDLVKSF"
     gene            6610..7758
                     /gene="thiO"
                     /locus_tag="VG272_07070"
     CDS             6610..7758
                     /gene="thiO"
                     /locus_tag="VG272_07070"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HWF13479.1"
                     /translation="MKTYDIVIVGGGIMGGSIAFELAKQKLNVAVLDRQELMHEASWA
                     AAGMLSPAPDCPAAIPAVPLGRASLAMYSQFVDAVERASGMSTGYRTGGAVMVISSGD
                     AQIELDKLVALHRRLGLACDPLPLEKARIMEPALGKDALATALLPDECSIEPRPLSAA
                     VLGAATATGVTLLPGTEVVSLALDGKKCIGVKTSAGETIHASQVVLAAGCWSSQILNA
                     VPYAPTIPLRGQMIALRHSGTPIRNVLRSERGYIVPRGRMSPQTLILGSTIENAGYEK
                     CVTSGGLEKILAAANALVPELAKAEVIETWSGLRPGTPDQLPILGPADIGGLVFATGH
                     YRNGILLAPITAKLVGEWITEKKTSMEWETFSPLRFLRPELNLSTHIS"
     gene            7856..8329
                     /locus_tag="VG272_07075"
     CDS             7856..8329
                     /locus_tag="VG272_07075"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWF13480.1"
                     /translation="MQPRIDYGKYAHGALNAMLSLEKYISECGLDPKLIYLMKVRASQ
                     INGCAYCIDMHSIDARAQGESEQRLYALDAWRETPFFSDRERAALEWAEAITLVSETH
                     VPDEVFEKVKKYFSEKEIVDLTIIATTINMWNRVAISSRAVPGQYKPAKAAAAKP"
     gene            complement(8679..9512)
                     /locus_tag="VG272_07080"
     CDS             complement(8679..9512)
                     /locus_tag="VG272_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13481.1"
                     /translation="MRINWLLPFVIMSAVSSGAVYGAQMPAGPGQAAPEQLPPAQAAT
                     AQTVSTPTAAASTAKGSPERYVVPSGTRLPLILHNAVTTRNAKPGDPVYLETLFPIVI
                     DNRILIPAGSYVQGEIQEARRPGKVKGTGEIRMRLNSMILPNGYVVDFNAVPTNAGTG
                     GNEATDSEGKIHGDTDKGSDVGTIAKSTGIGAGVGGIATQSAKGAGIGAGVGAAAGLA
                     AVLLTRGPELELPRGTTVDVVLDRTVYLDASRVNFTEPGRPSDLPGPPSREPTRSRSP
                     F"
     gene            9732..10538
                     /locus_tag="VG272_07085"
     CDS             9732..10538
                     /locus_tag="VG272_07085"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWF13482.1"
                     /translation="MKKLILFLCFAALIPARASWAQVAGANQVGTSMGHVHFLVPDVE
                     AADKFWVAMGGVQEKLGTNDVFKFPGVLILVRKGDGTMPSVGSVVGHIGFHVPDTTAA
                     LARWKAAGLKTEVGANPGQGFVWTPDNLLRVEILEDKTQTVPIAFHHVHFYIADATGS
                     NVLEMQSWYAKMFGAKPGKRGLFDAADIPGANLTFTKSDTPTVPTKGRMLDHIGFEIV
                     GLEAFCKKAEANGVKFDVPYTKRPDLGIALAFMTDPWGTYIELNEGLDKL"
     gene            10756..10956
                     /locus_tag="VG272_07090"
     CDS             10756..10956
                     /locus_tag="VG272_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13483.1"
                     /translation="MAKLALVYGMRLLPADELGELGDATITLKDGRTTRVTMHLIDGS
                     KEQIEETLRQSMEAFFEIYPEI"
     gene            complement(11215..11538)
                     /locus_tag="VG272_07095"
     CDS             complement(11215..11538)
                     /locus_tag="VG272_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13484.1"
                     /translation="MDERQFYFEKQETKTIPLTCPACRKEDNYPIRWIVRTKKNELPR
                     GAGEEDKKRFALARSYMVRVDELVACRKCRKRFELTGQSVVLVTSNAVAPGSPDFDPE
                     NFGNR"
     gene            complement(11575..12333)
                     /locus_tag="VG272_07100"
     CDS             complement(11575..12333)
                     /locus_tag="VG272_07100"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWF13485.1"
                     /translation="MNHLPTIVSCSVAAAAGIAAFGAVVPSSELFGPTLRHTESSRKI
                     ALTFDDGPNPAITPQLLDLFERYSVRGTFFLIGKFARACPALVKEIYVRGHLIGNHTD
                     THANLIWETREGIRSELAHCQDAIASVTGTEPPRWMRPPYGYRSPLLRREIRRAGIQG
                     VVMWSKICWDWKPQPPQRLIKRLASVARPGRSQGDIVVMHDGDHRFLNGDRQHVVTAL
                     EHWLPRWRDAGMEFVTMESSSAVEMADSTRPPRA"
     gene            12528..16151
                     /locus_tag="VG272_07105"
     CDS             12528..16151
                     /locus_tag="VG272_07105"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWF13486.1"
                     /translation="MPGKKIAMLFLGVLALLACVCPPLAAQDNPLSTGELADSQLSGI
                     VHAADGTAIPGSTLRVTQTSTGKAWVTWSDEDGKFEFPALPEGHFRIEASQLGFAPVT
                     QELDLVSGTKTPADLKMNVGQLSAITAPQAGTTETATTNAPPKDEGAKPAASSTPNAG
                     SEPNHAQNPPATSANNSTPSSPGGKAGNVAGSQRSGGNGQGGGRRSFQQLGLNGKNQS
                     SLETAPDDQNAPEAGGQLGQAASADAVQMTGTVAMGQIAPAAGQQAGENGPDTRTGMD
                     GDNNSIPGQSSPGGGGGPGGVGGGPQGGFGRGMRGGGGPRGPRGGPQGVAALWGAQRV
                     MRQRINRVHYSFYDTYGASPFNARPYSLYQANPPKISSWTESAGFNMGGPLKIPHIYD
                     GTDKTFFYINFGGTWSRTPVDQFATVPTVAERNGDFSADNVQLYNPLSNLNGPRGLMT
                     NTGCYQATASSPLPPAGTCIPTNLISSQAIQLLGYIPLPNIPVTNFAGQTFNYHLQTN
                     VPGISNRLNVNVTHQISAKLSLQVNYNFSDATSHSLNDFPEIEGDVFTRGQSAMIALT
                     QNWTKTFMHTSQFYFSRNRSLGLNGFSNVTDISSQIGLNGISPSASDFGLPSINFTNF
                     SGLSDPTFYLSRSQTYRYVDSLRWMKAKHTITVGGEVRKVDINRDTDPAPNGQFSFTG
                     LMTSQLSAAGTPVNSPANCQTSTASGPCNGNDFADFLLGYPANTKVQYGDTATYFRNW
                     GFIGYASDDFHAFPKFTVTYGVRYEAFTPPTEVNGHIANLAVSPDFTQVQCVTPVATG
                     NCIAGPTASLFHGHYNNWAPRIGIAWQPPGKWFSGQHQLTFRAGFSMFYVEAYLNTLS
                     AEMANQPPFATASTLTQRNFTTAPLSFQTNLSTALPDSVTNTVGVNPNYQVPYAMVWN
                     ASVEYNLASNAFLEVMYTATRGIHLDELLGFSSVTSGTTNAAGFTYDTTGAFSNFHAL
                     QVRVQKRMSHGIMVMGRYTYSKSLDDASTIGGGGTTVIQNNADPRGDYGLSSFNMTHQ
                     FLGQYVYQFPFGDRRRFAVKGWQKHVFGSWYTTAIFNAHSGTPYTVRVFSKNQACQNV
                     PGTNSERADQTGIESLSNPSTLEWFNTSAFSIPTVSCFGDAPRNSVIGPGAFSINTAL
                     TKLIPFGRDGLRRLDLTWSVANVLNHPNYTGLSTVLGSRTFGQITSVGSMRGMTFSTR
                     INF"
     gene            16151..18424
                     /locus_tag="VG272_07110"
     CDS             16151..18424
                     /locus_tag="VG272_07110"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWF13487.1"
                     /translation="MTARWIFAKRMIAAAAATSLLAAPLGAQSSPAQAQTSQPQPQPQ
                     IQQIPVNPRQGPTPPPPANKTAPDGKATIRSTVSLVEIDVQVTGRDGKPVKGLKQDQF
                     SITEDGKPQKISTFEYNDIEQIETAGSVDTAPVTVPLGTDSTPEEIKTVVRDHRMIVL
                     FFDLTSLQAEDLLRSTRAAQKYLQEQMTPADLVGVVAFGNTLKVVTNFTNDRDLLKQS
                     IDALVPGHEAALAALADAATAANGETAVTEDTGAAFTADDTEFNIFNTDRKLAAVESL
                     CEVLENIPGKKSIVQFTSGVTQTGEENRSELIAATNAANRSNVSIYSVDSRGLLTATP
                     GGDASAGASGGNALFTGATVISQSQSRQDSRETLATLAGDTGGRSFFDVGDFGKVFQS
                     VQNDTSGYYLVGYYSTNAATDGAWRRIHVKVDQLPAGSRIRAREGYYAAKNFGIFTTE
                     DRERQLENAFQSGNPEVELPVAVETADFRLNANQIFVPIAAKLAPSALQWAQKRGSHE
                     TSFDFAAEVRDAKSNRVVGALRDTVSVKIDAEHFQDMQQRALLYQGGIILAPGEYKLK
                     FLARENESGKIGTFEQKLSLAAPQPDRLQLSSLLLSSQVQALQNMAQIKTQALAQDAK
                     MKSSPLDVGGERIIPSVTRVFTGEQTLYIFFQAYVPQKADSNSIRAGLVFFRNGLRLS
                     DTPMISATEFDDKSRTASFRVSIPLADLATGRYTVQAVVVDAGTTYTAFARNYFALRA
                     APKPAASAIVPPVPGNN"
     gene            18637..20052
                     /locus_tag="VG272_07115"
     CDS             18637..20052
                     /locus_tag="VG272_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13488.1"
                     /translation="MRHVASIFILILWSSVFMYAQEDHVRGVRSGPALPEVIDLNVSA
                     GTPLPVLLDNEVRIKKAGQEVHGKIAEPVYVYDKLVVPTGSDVLGKVTRIGGVPALKR
                     TYAALNSDFSPVRDVEITFNRLILSDGSEIPLHTRVSLAPQGVLNFTTATEPKNGSKS
                     NKQNAAVKLASEKVSEKKQEVNREWNLAKEQITAPGKIHRLKRILISELPVHPQYIEA
                     GTRFNAELIDPLHFGTESKSAEELRMIGTTPAPGSIVHARLETPLSSETAQKSESIEA
                     VLTAPLITDNQLVLPEGTLLKGSVLQVRPARRLHRNGQLRIIFREIVPPNSAQQKIEG
                     SLEGVEASNSADLSLDSEGGAQATTPKTRYLTTGLTLALAAASLAPDRDAGSNGLSVL
                     EVGRQGATGASGFRVVGFTIGLLVHSRPLAAGFGAYGAARSIYSNFLSRGQEVVYPKD
                     SVMAIGFGSRVATTQKTSPPH"
     gene            20329..21537
                     /locus_tag="VG272_07120"
     CDS             20329..21537
                     /locus_tag="VG272_07120"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWF13489.1"
                     /translation="MDSVLAVLLHLKSMFRLSNLLLIFPVGTAFLIVLLMARRAWRVY
                     REQRYDAVSFKIHGQWRNIVRGEIPPHEWRNNSLKIEILQSIVIGEINGVTDKDRAGL
                     QKFLRESGLLSRCIERVLNESGWAKRHAMLDLGSMRVPEGIDSLVETLDDWQLDTRIT
                     AVRALGLTGLPEAAGPILEAHMVGGLKVPTAPIADALMRCFNDRPEALLPYLRRSMGE
                     SRVLLARVAGELSTPQMADEIIILAEDPEPEIRASAARALAALPLPLAIPVLAKLIRD
                     DAWFVRLRATSSLNQILHPRVIPILLEAIRDPHRLVRIRAASALAKFEHETMDILQSV
                     VDSRDKYALHAMISALDLGGGFGNVLALLADPEQHNEAAASLLDALRKGAAGLWTMKP
                     ADPAVEAVFP"
     gene            complement(21748..22977)
                     /gene="pobA"
                     /locus_tag="VG272_07125"
     CDS             complement(21748..22977)
                     /gene="pobA"
                     /locus_tag="VG272_07125"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HWF13490.1"
                     /translation="MSKLRVVPIHIAYDEGETVRTQIGIVGAGPAGLVLAHLLHLQGI
                     ESVVIESRSRKYIEERIRAGLLEQGTVDLLIETGVGKRLQREALTHHGIELRFNRRGH
                     RIDFNDLTGGKTVTIYAQQEVIKDLVAARLEAGLPLIFEASDVSVHNFDGANPSIRFR
                     VGGEEQELHCDYIAGCDGFHGICRPSIPAGVLTEFERIYPFGWLGILAQAPPSSPELV
                     YNYHERGFALLTMRTTEVSRLYLQVPHDEDISAWSDDRIWSELRARTAADGNWKLNEG
                     AIFQKGITGMRSYVVEPMQYGKMFLAGDAAHIVPPTGAKGLNLAVADVRIMAQAFSEF
                     YKTGKTEPLQRYSEIVLRRVWKAQRFSWWMTSMLHRFDTDNAFDGRRQIAELDYVTSS
                     RAAMTTLAENYVGLPWD"
     gene            complement(23045..24091)
                     /locus_tag="VG272_07130"
     CDS             complement(23045..24091)
                     /locus_tag="VG272_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13491.1"
                     /translation="MVAFELAQLHEKAGTISEAVRWFTTAAERFRRPQWKQKAEEGLV
                     RLGAPVPVAPAIPTGVAVSETTSETVAQLEPIISEFSEEDSNETEQEMTEGTQETVFE
                     QPTGEGAVAPAVQPPEVGPDRRRRRRGRRGGRGRHKGVRTAAPSTLASASAAAAVPAA
                     PPAPPSMFDRVAAESRPFPVARPSVSADIAESRVREAEPVRSYETTHAGPILSARVRS
                     GDPALSSRLAQLESQLRRLLACSIRSLEQADDAPAGPGVFLITDTDQTTYYYAEACQT
                     LRVGIGNMLRSGRGTREGSNLKEKLAEHLGINEARVGKYLKEHCGVRWLQLDEGAPLL
                     AHFAVAVLRPISND"
     gene            24251..25552
                     /locus_tag="VG272_07135"
     CDS             24251..25552
                     /locus_tag="VG272_07135"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HWF13492.1"
                     /translation="MGVCCIWLPVAGTARYHRALTLSPATLSEFTRLAAYAIFLLSYL
                     VFAIGKFPGMKIDRPGAAIIGAVLMVAFRAVQPADSLHFIDFGTIVLLFSMMLLVAYL
                     HLDGFFDWVSEWIAARISPRHLLPAIIFLSGTFSAFFVNDIICLIMVPFTLKVSRRMG
                     LPPLAYLLAVATASNIGSVATITGNPQNMLIGSYSGMRYSRFLFHLGPVAFFGLLVDW
                     LVLWFIYTRHETPEARPVDVAVSASGSHGTLMKAGIVILLVLAGFLAGLPPAQVAAVG
                     AAIMLMTRRHDPRQVYDEVDWGLLVFFVGLFLIVGGAENVGLTERIVEVGRRWNLQHA
                     YTLTIVTAGLSNIVSNVPAVMLLKSVVPRFANPQQGWLVLAMASTLAGNLTITGSVAN
                     IIVVERAKPEVKITFGQYLRAGVPITLITLAFGWLWLMWAS"
     gene            complement(25728..26552)
                     /locus_tag="VG272_07140"
     CDS             complement(25728..26552)
                     /locus_tag="VG272_07140"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HWF13493.1"
                     /translation="MLRLETETGWWLVTHPDHARLAGAFAERWGNDLFLPPEPREHVM
                     RGIARHDDGWATRDAAPQITRQGKPSAFSTELVGKYSAFEEIDLADYLAVRDRAVRLI
                     AAEDPYAATLISMHTYSLLHDHADRSTIAPSQLPLLDRFLEDQRELQHSLRQQIAANP
                     KLKPEDKSGDAILDHFHLLQANDNLSLLTCVDFKNPANLLHGLPTRDGRRVPVEVRSV
                     GVRRFILKPYPFAERTITFQFPARHANGKLFSTAKELQDAFHSAQQEMLSVMVSAD"
     gene            complement(26593..27384)
                     /locus_tag="VG272_07145"
     CDS             complement(26593..27384)
                     /locus_tag="VG272_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF13494.1"
                     /translation="MTQRTITILNAVAVFVAFLLLWQLILWVFRVPRFMLPSPYAVAL
                     SVFARFPSLMSSLAITAEESAGGLIASIIVGVLVALVFAQSPWVRKMLYPYTLLLQTV
                     PIIAIAPLILMWMGAGMLAVTLIAFIISLAPIIANTTQGLISVDENLVHLFLMHNASS
                     RQLLFKLRLPNAIPHLFVGMRIASGIAVIGAITGELFAGSGRVGEGGLGYSILYASSQ
                     LQTEYLFALVLAATVLGFGFLFLIMFLEWYFLHKWHESARAAEIE"
     gene            complement(27381..28154)
                     /locus_tag="VG272_07150"
     CDS             complement(27381..28154)
                     /locus_tag="VG272_07150"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF13495.1"
                     /translation="MNANVGVPEIEIAGVTKRYGDARAVLDSINLVVEKQEFVSIIGP
                     SGCGKSTILKLIAGLSPPTSGTIRVDGMTPQNARETVSFVFQDATLLPWRTVNENIAL
                     GMELERVPKDRREKETASLLDLVGLRQVSQSYPRELSGGMKMRVSIARALAKHPRVML
                     LDEPFAALDEMTRDRLNEEILRLRTEQQWTAVFVTHSVPEAVFLSDRIVVLAPNPGRV
                     YGDFRVDLPMPRTSDVRGSKEFDALASRVTHTLRETILQ"
     gene            complement(28151..29866)
                     /locus_tag="VG272_07155"
     CDS             complement(28151..29866)
                     /locus_tag="VG272_07155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWF13496.1"
                     /translation="MSPPKWVDCGEGVRLERGVCARMRDGLELISDHYYPPQSGALPT
                     LLMRQPYGRDIASTVVYAHPIWFARHGYNVVIQDIRGRGNSEGEFYPFRNEGPDGADT
                     ISWLRTRPESNGRVGMYGFSYQGMTQLLAAAERPEGLQCISPGMTACDLYNGWFYQGG
                     VLRLASTLGWGLQLLKEDARRKKLREPSDRLEQAWAQLASQTSVLSIRAHPALRGEGL
                     PQYVLDWFDHDTPDDYWAALDVSRHPEKICIPALHISGWYDTFLRGSVDGFLALSKSA
                     GSEFARQHQYLLAGPWVHIPWGDRIGSADFGPEALIDTDAILLRWFDHWLKDSGSFDK
                     DPRIRHFVLGENRWRDAEKFPSDAEHILFLRSEGRANSRKGNGTLSVSSRSANEPCDI
                     FVHDPEVPVTAPGGPAAASGQFDQAALEMGNNLLVYSTGPLEKSLRIFGKPRIVLYCA
                     TSLKNADVTAKLVRVRPNDAAEFICIGVARSTHLFAETNYEADKIHRWEFSLEPTSCR
                     FEKGDRIRLEIAGSAFPLLDRNPGSDVPSCAATSWDWRRSTHSIFHTREYASAFYLPV
                     TEEPQ"
     gene            complement(29863..30657)
                     /locus_tag="VG272_07160"
     CDS             complement(29863..30657)
                     /locus_tag="VG272_07160"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HWF13497.1"
                     /translation="MKTWIPDGRNFAYLNWKQVDALPRDTTLLVLPTAAIEQHGHHLP
                     IATDTLINNLLLGHALRNLPAELPVYALPPVHYGKSNEHIGFPGTLSVSSSTFMAVLR
                     DLASSLSKAGFRKLVLYNTHGGNTALIDVMARDLRAEFGLQMFAMHGSGGISFEGLNP
                     QERAYGFHAGEVETSFLLASVPELVDQSAYTSNYIADVTKPALLLPEDAPATFAWLTK
                     DIAASGVMGDPRPATSEKGARWLEQAATRLAAALTEMYHYTDLSTS"
     gene            complement(30654..32030)
                     /locus_tag="VG272_07165"
     CDS             complement(30654..32030)
                     /locus_tag="VG272_07165"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWF13498.1"
                     /translation="MEPKHISELRLLITDPARVILNPQIVEQLSKDFYWYSPVLRKQL
                     DGKIGEVVVQPRSAEEIQKLLRYCYSNHIPITARGAGTGNYGQAVPLEGGVVLDLSRM
                     DHIEQIQTDGVAICQPGVRLGDLENEARKSGWELRCYPSTIIKASVGGFVSGGSGGIG
                     TVAHGGLRDFQTVRALEVVTMEPEPRVVLHEGEAIHEILHAWGTNGIITHIWLALTPA
                     VEWSQCIAAFDTFSAAFDFAEKIATASAWIKRLVTVFEWPIPSFFTPIRQYAPAGKTL
                     IFFMIASSQLGELEIAARDTGGSVTQSGIYPGLNARPLLSDYTWNHTTLWAMRSDDAY
                     TYLQCGFNPDTVRDQMKQLRERFGQEILFHMEFMKNGEGRVVPGAIPVVYFKSEERLN
                     DMIDFCRKIGVFVANPHVNNVEGGGRYRSDNVQLLAKLRYDPKGLLNPGKMVTFHSES
                     TKAGAITK"
     gene            complement(32047..32595)
                     /locus_tag="VG272_07170"
     CDS             complement(32047..32595)
                     /locus_tag="VG272_07170"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HWF13499.1"
                     /translation="MAFTEILRSRGAAVDEERMREIYRFTARSLITTRPVPFGALIVN
                     TRTGERLMRATNAVFRESDPSAHAETRTVRLACKKLKRPSLAGYTMYSTCEPCAMCMA
                     NALWAGLDRVVFGATIDDANRFCLQIHIPAKEVSSRSDMPCIVDGPVLRELCNSLFTH
                     PNMRKAFRTWSSSKTKSAKSQI"
     gene            32730..33713
                     /locus_tag="VG272_07175"
     CDS             32730..33713
                     /locus_tag="VG272_07175"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWF13500.1"
                     /translation="MAKRNFTILCAVTFVGLFISGCHHPAADNGLTKVVLQADWYPQP
                     EHGGFFTALTKGFYKDEGLDVTILPGGPYITVEKQVAAGAAQFGMGASDKVLEASAEG
                     QSLIAVAATLQHDPQGIMVRKDSPIHSFADLNGHSVAIKVGESAWFEYLTKRYQLNNV
                     RVVPAMMNVANFVADPQYIQQAFATSEPFFAHQAGIETRVLLVSDAGFNPYRVMYTTK
                     DFVQRHPEIVAKFVHASVKGWKEYLNDPVPANALIAKLNPALNPEWMQFTWQQLKDGH
                     FVAGEDPTGAQIGQMDPKRWATMYEQLVDLKMIAKPIDPTTAYTLQFLGPQ"
     gene            complement(33795..34886)
                     /locus_tag="VG272_07180"
     CDS             complement(33795..34886)
                     /locus_tag="VG272_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13501.1"
                     /translation="MKFSTIGRLAAGLSLAFVGACFLSGSYAQPKPADSGYHLLKTIS
                     LPAAPGGGEYYDYISVDADARRVYVSHGTEVVVLNADDYSVVGKIEGLSRSHGIALAK
                     DLGKGFITDGDSREGATVQEAVVFDLKTLKITGRVKTGQLDTDAIIYEPVTKHIFTFN
                     GDSHNTVVIDAVKESVITTIDLVGKVEFPAVDGKGLVFDNNPEKNDIVVIDAKTNTIK
                     ARYPTAPEGNPTSLAIDQKTRRLFSGGRGPQFMIMMNADTGKVIQSYPISAGVDANAF
                     DPGTKLVFVATREGMMHIYHEDSPEKLSEVEAVKTEYGAKTLQVDSKTHKVFLSTSDF
                     NPPAAPTEKQPHPLATPKAGNFRVLVFGK"
     gene            complement(35362..>38824)
                     /locus_tag="VG272_07185"
     CDS             complement(35362..>38824)
                     /locus_tag="VG272_07185"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HWF13502.1"
                     /translation="AQLPLDADLRDLHADIHFNPVTSEYRGTIGYSDGRIMAETLKPI
                     SHRLQLNFALDRSGLAADPITIATEQSTLNLHAKLTNYDSPKIDGTYDGVVSTIELAR
                     YTESKSIPAGKVAMTGNLHYENVPNKSVMDTAYVDGRLNSEEFVLDIGQMRTNPKSIR
                     AEFKLQDGNLSVKNAEADVLGGHVSADYDLSRISGNSSSRLRAIVRDVSLQAMDRTAA
                     IRNGEAAKISGRVNGTAEATWTSSVQNAMARVHFDIRNPRPPTVGDRTIPLNGLIDVA
                     YDGARNTASFGQSYLQTGTTKLSISGILSNRSQLNVQANTTDLHEVTALVSAIQSGSS
                     PSANAAASSLPTLGGAARFSGQVSGDVKQPRIKGDLSANDLQVRNAHWRNFHATLDAA
                     PSSITLQNGSLIDPQNGQINFNGHADLTSWSFTPTSPLTLQLNARQISISELQQFLQA
                     QYPVTGTVAANISIHGSEQNPQGEGTLQITRASAWNEPIQNLNANFNGDGNSIHSEVQ
                     LTIPAGNLTAAMIYQPKTQSYEINLNTTGMKLERLESVQARNIGVAGDLTLSAQGRGR
                     LMNPEFAANLQIPKLAIRDQTVSDVNAQLSLADQHATFTLNSKADQGSIQAKGNVGLT
                     GDYPATASLDIRAVPVALVLARYLNKTQQIQGQTEIHINMEGPLKNPNAITAQLEIPT
                     LDVNYQQAKLGLVRPLRATYRNGIVNIDQAELKGTGTDLNVKGIIPVKASVPLNVSAN
                     GALDLSVLQAFTTGMKSSGNINLNMSARGDLSNPAMQGKVEIANAAVSTETLPVSIEG
                     MNGEIQINGNRLEVAQLSGAAGGGSVAVRGFMTYGKQSNFNLGLDAKNVRIRYPEGIR
                     SVLTGNLSLAGNPASSQLTGRVLIDRLSFTQQFDLATLLGQLSNDTPATTSSPFETNM
                     KLNVAVATAQDINLASSKVSMGGAANLTLSGTMASPVVLGRANLTSGEMFFMGKRYEI
                     ENGTIEFANPVRTVPVVNLYVKTTVQQYNITLNFVGPVDRLRTNYTSDPPLPPADIIN
                     LVAFGKTTEQAATSPSTPATLGAESVLAQGAAGQLSGRLEKLAGISQLTIDPLAGNSQ
                     SNPGAQVAIQQRVTGKILLTFSTDVTSTENQAIQVQYQVKKGLSVSVLRDQYGGYAAD
                     MRIHKTF"
BASE COUNT         8982 a        10403 c        10564 g         8875 t
ORIGIN      
        1 aaatttttga gaatgcgggc gtaatcagcg tgaaaattaa aattaataaa ccaccacaac
       61 gcgctgcgaa tctcagcatg aacaaaaccc cctgaaatta ttttttggat ctggacgttc
      121 tgtaaatcat cgaagcctga aataataatt tcatctattt tttcagtgcc aagctaccga
      181 ggacctggaa cacaacccat atatcggcct gccgaggacc gatattataa tcagaccaaa
      241 aacggcacaa tacacgattt cctcctgttc atattctagt aagggattcg aagatgcctc
      301 attcagaaat gagcatcctt ttttaattca tagaccaccc tccggacttt tctgtcatcc
      361 gatttgatga cgtgaacagg cagacccccg ttgataaagt tgataaaaat gatccgcctg
      421 caaattcttt gagcgaatgg cgggaagaaa caaagagcgg agaattcaag aaatgaaaaa
      481 aacttttcga gcgcctgcga ttgtggttat ttttctttta agcgccattg gaatgcgtac
      541 aacggccgca tcggcggggc aaacccggag cgccacgtca ggttcatggg ccgattccgt
      601 gcgcaatgct cctcattttc tggttcgtct cgaccaggaa ttaaatacag gaaaaaacag
      661 aaatcacgaa aaatttcagg tacaaacgat tgaacctctc gtggcttcca atggacacat
      721 attcccggtt ggaaccaaaa tattggggca catcagccgg atagagcccg gaggattaac
      781 cgggcgcgcg cgagtatggc tgactttcga tgatgttgag acggcgagag ggacgctccc
      841 aattgttgcg gaagtttcaa gcgttcctgg ggaattcagc gtgcagacgg gaaaaagcaa
      901 ggagggtgaa atcacagcgc ggacaggcaa aggagccgag gtggcggaag ctacagcggt
      961 tggagccgcg aagggcagcg ctaccggcgt gacttcgcac aacggcaaag aagccgccat
     1021 aggtgccgct gcaggtggta tggccgcatt tctggcgtct tcgggaattg gccaggaaat
     1081 tgatttgcca aagggtacga agctcgagct ggttcttgat cggcccttat atatgaatta
     1141 gtcgtgcgtc agtgatatgt ttgccattat ttccacgccg cgtacacagg ttttccaacc
     1201 agcagcgcag gatttgctcc cgggcgcggg cggaatttct gggcgcggcc attgccgact
     1261 tcggcgacat aaaaatttcc ttcctgatcg acgctcatgc cgtgcgtgtt gaagaggccg
     1321 ccgggccaat cggccagcgt gccccacgag tagagcaaat gcccttcgag gtcgtattcc
     1381 aaaatttttg agtttctgtc gtcagcggcc cacaattttc tatcggcgga catgtacaga
     1441 aaattgatgg acgaatattt gtcgtcggcc cattgatcca cgaatttccc gttttcatcg
     1501 aatacctgaa tgcgatggtt gctccggtcg acgacgtaca cgcggtgcgt gacgggatcg
     1561 gcggcaattc catggacgac aatgaacggt ttcatgaatt acaaatttac cctgcccgcg
     1621 agaaaccgcg aaactgacag tcagcaaaaa ttcaagtacg aaaaaatgcg ctaaccgcca
     1681 aattttagaa attcagggta tagtatggct tcaagcccaa gctcaaggag gaaatacgat
     1741 gcgaatcaaa ttaatgcaat cgctgatcgc tgtgtcaatt ttgtctgtga caattacggc
     1801 gggtgtggcc gcgcaacaac aagcaacacc gcctccgcca tccaaattta aattgatgtc
     1861 gtctgcgtac accgaagggt ccatgatccc aacgcaatac tcgtgcgcgg atccaaacgc
     1921 agcatcgccg gcgctttcgt ggacgaatcc tccgagcgca gcgacaagtt tcgcggttat
     1981 tttccatgat acggacgctg ccccgatgaa gggctcgatg gacgtaacgc actggatttt
     2041 ctggaacgtg cctggcacat cctcttccgt ggcagcagga gtgaagcccg actcttcacc
     2101 gaacggaatc gtgcaaggcg tgaatatccg aaaggtaaat gggttccagc cgccatgccc
     2161 tcctccagga gcaacaccgc atcattatat ttttgaaatt tacgcgctgg attcgaagct
     2221 tgatttggcg gcaggatcgg cccgcaccga tctgctgaag gcgatggacg gccacgtcgt
     2281 tggtaaggct acttacgtcg gcatatttgg ccgttaaatc gccgcgccct tcagggaata
     2341 agcgacgagc atttttcatg tccgtcgcat tccaaacaaa cgcaaatagc cgtcagagct
     2401 acaaacttgt gggctctgac ggccgacctc ttttcataac gttacttttt ctcagcaacg
     2461 atggactgat tcatattgca gcttcgtcac cagcaggcac gaaaaacctg ggaaggtaat
     2521 cctgcatctc agcatggtat ttcacgggcg gtactgatag attcgaaggg aggcgccggc
     2581 cctgcgtccc tgggcccgca cgcaaaccga atggctgatg caactcgatt tgaaactaca
     2641 aaattgtctc cagaccgcgt cctgattgtc gaggacgagg aaaatgcccg tctgggttac
     2701 gaagctctcc tgcgccgctg gggttatgaa gtgctcggcg tgggcagtgc tgaggatgct
     2761 ctcgcgaaat tttccgagtt cgcgcccgca gtactgattg ccgacgtgga gctgcccggc
     2821 atgaacggcc ttgatctttt ggcgcggctc ggcgacgaac tgcaaaacgt tccggccatt
     2881 atcatcaccg gcaaagggag cgaagaacgc atcgtgcagg cgattgaagc cggagcgttc
     2941 tggtacatcg aaaagccgct aaaaggctct gtattgcacg tactgctgga gagggcgctg
     3001 ggacgcgtcc gtgaccgcaa gcaggttgca gcgctcacga ggcagttgcg cgaatccgga
     3061 aaactgggcg atctcgttgg acaatcgaga acgatgcagg aagtaatgca cctggtggag
     3121 caggtggcgc cgtcgtccgc ttcaatcctg atcaccggcg aaaccggatc cggcaaagaa
     3181 attcttgcgc gcacgattca ccggctttcg ccgcgagcag accgtccgtt catcgcgatc
     3241 aattgttcgg ccattcccga gtccctgatg gaaagcgaaa tttttggcca cgagcgcggc
     3301 gcatttacgg gtgcggccga gcggcggctc ggatgtttcg agcttgccga tggcggcaca
     3361 ctgctgctgg acgagatcgg cgaaatgccc gcgccgactc aggcgaaatt actgcgcgtg
     3421 ctcgaagatc ataaagtgcg ccgcttgggc agcaaagtgg aaacgcctgt cgatgttcgc
     3481 gtactggccg caacaaacaa agaacccgaa gtggcagtgg ccaagggaga attgagacag
     3541 gaccttttct ttcgcttgaa tgtatttcac attcagctgc cgccgctgcg cgatcacaag
     3601 gaagatattc cgctgctggt agaacatatt ttgcgtgaca ttaataccaa gcacggccgc
     3661 catgttcgcg gcgtaaatcc cgaagtgctc gatcagtttg cgggttattc ctggccgggt
     3721 aatgtgcgcg aactgcgcaa cgtgctagaa cgagccgcga ttgtcactga ccgcgatctg
     3781 attggccgcg cgagcctgcc ggtggatttt gggcacgcgc ctgccgcaag cgcctccgat
     3841 ctggcatcgt tgcgattccc gctgggcact accgtggagg cagccgaacg tgagctgatc
     3901 ctgcaaaccc tctcatctac taataacaaa acacgctccg ccgaattgct aggaatcagc
     3961 ctgaaaaccc tgcacaataa gctcaaggaa tatgaggctg cgaaaactcc ctcgaagtga
     4021 gggacagggc acctatgcaa ctgcgcctga agacaaagat tacgctggca acagcggcgc
     4081 tcgtgctcgc cgtagtaggc gtgaattcga ccctctatgt catgaacctg tcgcgccagg
     4141 tgattcgcca ggcggacgag cgcgcgcaac tggtttccaa acaggttttc tttcaggcgc
     4201 aagtcgctct tgccgatgcc gcaaaagcgg gagaagctcc cgactccaat agctctgaag
     4261 atctgcgcga atatgcgcag aaggcattcg atgaaagcgc gtcattggcc tcttcgatcg
     4321 atgcgcagct cggctactct cccctgatct atgaaatttc catatccgat gtgaacggta
     4381 aagtgcttgt ttcgagcgat aaatccatgc gcggaaatcc ggagccgata cgcgcaggca
     4441 tcgaacaact ggtttccaat ggatttgtaa agcaactcag ggttctttac agcctgcttt
     4501 atggaccgcc cctacgcgct tatgaagtta cctatccgtt ccagttaggg ccgccgggcc
     4561 agcaaattcc attcggagcc gtccgcgttg ccgtgcaaac cggattgctg cgcgcaagta
     4621 ttatcccggc gttgcggtcg gcggccttgc tcgccattat ttccgttggc gtgtccgtgt
     4681 tgctggcggg aattgtcagc agcatttcgc tcgctccact gaaacgtatt accgcccaac
     4741 tggaccgcat cgccaaaggt gaattcgatc agaagccact ggagcgcggt gacgaattcg
     4801 ggcaggtcag tacgaaaatc agccagatcg gaatgcaact gcgcggagtc cgcgaaattt
     4861 tcagcaactt gcgggagaac atcgatcagg tgctcggcgg cctcgatgac ggcttgctgc
     4921 ttttttcagt ggatgggcgc gcggtgatgg tgagtcccgc ggtggaaaga ttcctctcca
     4981 ccccggcgga tcaacttctg ggccggcgtg cggaagatat ttttcctcct gaccacgatg
     5041 tgcggtcagc gatcaaaatg cagaatggcg agttcgaacc ggtggcttcg gcggaagcgg
     5101 tgttgcgcca catcgacggc ccaccgcgcc gggttggcgt gagcgtggaa gtgatcggcg
     5161 aaggcgatat gcggatgggc acgcttgtaa agttcaagga tctggaatcc agagagcgaa
     5221 ttggcacgca gcttcaggtg tccgaacgcc tcgcgaatct cggcagaatc actgcgggcg
     5281 tggcccacga agtaaaaaat ccgctaaatt cgatgcgcat ctggctggaa aatctgaaag
     5341 caagtttgcc ggatgttgag ggattgccgc tacaggctgt ccgggttttg gatagcgaga
     5401 ttgatcgttt ggacagcgtt gtgaaacgtt ttctcgattt tacgcgtcca cctgatatgc
     5461 atcaggaaga agctaacttg aagggcaccc tggaagaggt cctggcggtg gaaaagcctg
     5521 agctggacaa ggcaaacatc aagctggaag caaaattggc atctaatgtt ccgaacgtgc
     5581 tggtcgataa attacttttg aaacaagcgc tcgttaattt aattttgaat gccatcgaag
     5641 ccatgcccaa aggcggtcag ctttctgttt cgctgaaccg ccgtgatgaa atggcggaaa
     5701 ttgaaattcg ggacactggc cgcggcattg cgccggaaca tcggcagcgc attttccagt
     5761 tatttttcac gacgagagcg ggaggcagcg ggatcggttt ggcgagcaca ttcagaaccg
     5821 ttcaactgca caacggttcc atagaatttg agtctgaagt aggacgaggc acaacatttc
     5881 ggatcgattt gccgctggct caccaaatgg agcctgctct ttcgcggcca cgcgattccg
     5941 gtgcggctgt tgcggggaga ctctagtgcg aaaatttaaa cttctcgcgt tgctttgttc
     6001 gctctctgct gtgatggcgg ggtgtgcgag caaacatatt gtgcgcgcgt cgccaccttc
     6061 cgtgagcgtg ccgccgccgg atgaaaccgc gcccatgcct gcgccaacta caccgccgcc
     6121 tggtcccgca gtcacagagg aacctccgcc ggaagtggaa tctccgatgc cgccgccagt
     6181 tccgggcaag cgtccagccg cgcctcggcg ccccgcgcca acggaatcgg cggactcgac
     6241 tccggccaag cccgcagcgc cgcagatttc gccgcaactt tctccgagag atctggaatc
     6301 aacaaaaaag aatacgacgg ccaatatcat gaccgccgaa cagaatttgc agctttccaa
     6361 tggaaggcag ctcagcgcgg cacaaaaaga tttggcggaa aaaattaagg gatttctaag
     6421 ccagtctcac gatgcgattc gttccgatga ctgggtccgc gcgcagaacc tcgctgaaaa
     6481 agcgcgcgtt ctatccacgg acctcgtaaa gtccttttaa ctcccctttc aatattaacc
     6541 acgaatgacc gcccgcctca gtcgcatgtt gaaagtgccg ctcaccttta ttgctgataa
     6601 gatacccgcg tgaaaactta tgacattgtg attgtcggcg gcgggatcat gggaggatcc
     6661 attgccttcg aactggccaa acagaaactg aatgttgctg ttttggaccg ccaggaatta
     6721 atgcacgaag cttcctgggc ggcagccgga atgttgtctc ccgcgccaga ttgcccggcg
     6781 gcaattcccg ctgttccgct ggggcgcgcg agcctggcaa tgtattcaca attcgtggat
     6841 gcggtagagc gcgcgtccgg gatgagtacg ggatatcgca cgggcggcgc ggtgatggtc
     6901 ataagcagtg gggatgcgca aattgaatta gataagcttg tggccttgca ccgtaggttg
     6961 ggcctcgcct gcgatccgtt gccgctcgaa aaagcaagaa tcatggagcc ggccctcggg
     7021 aaagacgcac tggccacggc gcttcttccg gacgaatgtt cgatcgagcc gcgcccttta
     7081 tctgctgccg ttcttggcgc tgcgaccgcg actggggtca cgttgcttcc cggcacagaa
     7141 gtggtttcgc tggctctcga cggaaagaaa tgtattggag tgaaaacctc ggccggtgaa
     7201 acaattcacg cgtcacaagt ggtgctggct gcgggctgct ggtcatcgca aatattgaat
     7261 gccgtacctt acgcaccgac aattccgctg cgcggccaga tgattgccct gcggcattcc
     7321 ggcacgccta ttcgaaatgt gctgcgctcc gagcgcggtt acattgtacc gcgtggcagg
     7381 atgagccccc aaactttgat actgggtagt acgattgaga atgcgggata tgaaaagtgc
     7441 gtgacttccg gcggtcttga aaagattctc gcggcggcaa atgcattggt tcccgaattg
     7501 gcgaaagcgg aagtcatcga aacctggagc ggcttgcgtc ccggcacgcc ggatcaatta
     7561 cccatccttg gaccagcgga tatcggtgga ttagtcttcg ctaccgggca ttaccgcaac
     7621 ggaattcttc tcgctcccat caccgctaaa ctggtcggcg aatggatcac tgaaaagaag
     7681 accagcatgg aatgggaaac attcagtccg cttcgctttt tgcgccccga attaaatctc
     7741 tccacccata tttcttagcc attgcaccat ctccatcctt agttgcatct attttaaaag
     7801 aagcattgat tcccgattta ttgcgatatt cgcattcggg gaagggagat tttgcatgca
     7861 accacgaatt gattacggaa aatatgcgca tggagcgcta aatgcaatgt tgagcctgga
     7921 aaaatatatt tcagaatgtg ggctggatcc taaattaatt tacttgatga aggtgcgggc
     7981 ttcgcaaatc aatgggtgcg cctactgcat cgatatgcat tcgatcgatg cgcgagcgca
     8041 gggagaaagc gaacagcgtc tatatgcgct cgatgcctgg cgcgaaacgc catttttcag
     8101 cgatcgtgag cgggcagcgc ttgagtgggc ggaggctatc acgttggtat cggaaactca
     8161 tgttcccgac gaagtctttg agaaagtaaa aaaatatttc agcgaaaaag aaattgtgga
     8221 cctgacaatt attgcgacca caattaacat gtggaaccgt gttgcgatct cctcgagagc
     8281 cgtgcccgga caatacaagc cggccaaagc agcggctgcg aaaccataat tttttagtat
     8341 cgactttttt aggtcgattt ttttaaatag aagcgatctg gctctgttga catcatggat
     8401 gcttcagaaa gtttcgtccg ttttgtggca ccagaattgt agctcgcctc ttcagaggcg
     8461 agggtttaga tgctggagcg ccaaaaaccc ccgcctctga agtggcaggc tacagcagat
     8521 cggcagcgga accaggaatt tcaatagagc caggtcaact taataaatag cctttgtggg
     8581 tcgcggtttc agccgcgatg ttcagggacc tgaaaatcaa gggccttagc cctgaagtcc
     8641 tatcaagtgt ccgcggcgag ccatttaaga gacgactact agaatggtga ccggctgcgt
     8701 gtcggctcac ggctcggcgg acctgggaga tcggaaggac ggcccggctc cgtaaaattc
     8761 acacggctcg catcgagata tacggttctg tcgaggacca cgtccacggt ggtgccgcgc
     8821 ggcaattcca gttcagggcc acgcgtgaga agaacggcgg cgagtccggc agcggcaccg
     8881 acgcccgctc ctatcccagc acctttcgcg ctttgcgtgg cgatgccgcc aacacctgcg
     8941 ccaattcctg tggacttggc aatcgtgccg acatcacttc ccttgtccgt atcgccgtga
     9001 atcttacctt cgctgtcggt tgcttcattg ccgccggtgc ctgcgttggt aggcactgcg
     9061 ttgaaatcga cgacatagcc atttggaaga atcatcgaat tcagccgcat gcgaatttct
     9121 cccgtgcctt tcactttccc gggtcgccgg gcttcctgaa tctcaccctg cacatacgat
     9181 ccagcgggaa tcagtattct attatcgatc acgatgggga aaagcgtttc cagataaacc
     9241 gggtcgcccg gtttggcgtt tctcgtggtc accgcgttat gaaggatcaa tggcaaacgt
     9301 gtgccggatg gaaccacata tctttccggg ctgcccttcg cagttgaggc tgcggcagtg
     9361 ggtgtggaaa ctgtttgcgc tgtagccgcc tgagctggtg gcaattgctc gggtgcggct
     9421 tgtccggggc cagcgggcat ttgtgcgcca tacacagcgc cgctcgacac cgcggacata
     9481 atcacaaaag gaagtaacca gttaattctc attcagattc tccgatgcga cttacgatgc
     9541 acgttcccga ccataggatg ccataacttt cattttcgga cgggaaggct tttcttgaca
     9601 tgcgttgtca ggtcacatac tgtcttgcga aacgggttgc aagccttaag tttcaaaaaa
     9661 gcggcggata tttcattcgc gaaggttgtt caacattttg agaaaactgt tggcatataa
     9721 ggagtaattc gatgaaaaag ctgattttgt ttctttgttt cgcggcattg attccggctc
     9781 gcgcgtcatg ggctcaggtc gccggcgcca atcaagtggg cacatccatg ggccacgtgc
     9841 acttcctggt gccggacgtg gaagccgccg ataagttttg ggtggccatg ggaggcgtgc
     9901 aggagaagtt gggcacgaac gatgtgttca aattcccggg cgtcctgatt ctggtccgca
     9961 aaggcgatgg cacgatgcca tccgtgggat cggtagttgg ccacatcgga tttcatgttc
    10021 cggacaccac agccgcgctg gcccgatgga aggccgctgg cttgaaaacg gaagtcggcg
    10081 ccaatcccgg ccagggattc gtgtggaccc cggataatct tttgcgtgtt gagattttgg
    10141 aagacaagac acaaaccgtc cccattgctt ttcatcacgt tcacttctac atcgcggacg
    10201 ccactggttc gaacgtgctg gagatgcaat cgtggtacgc caaaatgttt ggcgcgaagc
    10261 cgggaaaacg cggtctattc gacgcggcgg atattccggg cgcgaattta acgtttacga
    10321 aatcggatac tccgacggtg ccaacgaaag gccgcatgct cgatcacatt ggatttgaaa
    10381 tcgttggcct tgaggcattt tgcaaaaagg ccgaagccaa tggagtgaag ttcgatgtgc
    10441 cttacaccaa gcgtccggac ctgggcatcg ccttggcgtt catgaccgat ccgtggggga
    10501 cgtacattga gctgaacgaa ggtttggata aattgtaaat cgccaacggt acgggctggc
    10561 tccagcgaag caagagccgc cctaaatgtt cagagtaatt aatttcaaat cgccgcttca
    10621 tcgttttttc cgaacgatga agcggcgaag ttttttcagg gtattctttc gctcttgaaa
    10681 tttcggcggc attttatacg ggaatttgac gcagtgcgtg cgccagctta agcttatttt
    10741 ttacctggag gcactatggc gaagctggct ttggtatatg ggatgcgcct gctccctgcg
    10801 gatgaactag gcgaactcgg cgatgcgacg atcacgctga aagatgggcg cacgacgcgc
    10861 gtgaccatgc acctgatcga cggaagcaaa gagcagatcg aagaaactct tcgccaatcc
    10921 atggaagcat tcttcgagat ttacccggag atttaaagta gaacaggctt cagcccgttt
    10981 tcttttgaat tttttacgaa tgcaaaacgc agttttcaat tcagaaaaaa taaaaggttt
    11041 ttagactgaa cagtccgagc gagaaccgca gaatcgttgt gggttgtggg tcttaggttg
    11101 gggtcttggg ttgtgggtcg tgactttagc cacgacatag tccagcaaaa tgagcccggc
    11161 ttttgccgct gattttccaa tgttgcagtt atcatgcaga ctctgaatcc tctcctacct
    11221 gtttccgaaa ttttccggat cgaaatcggg agatcccggc gcgacagcat tcgacgtaac
    11281 cagcactacg ctctgccccg tcagttcaaa tcgcttcctg catttgcggc acgcaaccag
    11341 ctcatccacg cgaaccatat acgagcgcgc aagcgcgaac cgctttttat cttcctcacc
    11401 ggccccgcgc ggcaattcat ttttctttgt gcgtacaatc cagcgaatcg gatagttatc
    11461 ttccttgcgg catgccgggc acgtaagggg aatggttttg gtttcctgct tttcaaaata
    11521 aaactgtcgc tcatccatat gatccctgct ctctcccagc cggctttcca cggatcacgc
    11581 gcgcggcggc cgcgtcgaat ctgccatttc aactgcggac gacgattcca ttgtaacaaa
    11641 ttccatgccc gcatcgcgcc agcgcggcag ccagtgttca agcgcggtga cgacgtgttg
    11701 gcggtcaccg ttaagaaagc gatgatcgcc atcatgcatc accacaatat cgccttgcga
    11761 tcggcccggg cgcgcaacac tcgcaagcct ttttatgagg cgctgcggcg gctgcggctt
    11821 ccagtcccag caaatttttg accacatgac cacgccttga attcctgctc gacgaatttc
    11881 tctgcgaagc agggggctgc ggtaaccata aggaggccgc atccaccgcg gcggctctgt
    11941 cccggttacc gaagcaatcg cgtcctggca atgcgccagc tcactgcgaa ttccctcccg
    12001 cgtttcccaa ataagattgg cgtgcgtatc cgtgtgattg ccaatcaggt ggccccggac
    12061 gtaaatttcc tttaccagcg caggacatgc gcgcgcaaat tttccgatca ggaaaaatgt
    12121 tccgcgcacg gagtagcgtt cgaacaggtc aagcagttgc ggagtgattg ccggattcgg
    12181 cccatcatcg aacgtgagcg caatctttcg cgaggattcc gtgtgccgca gcgtcggccc
    12241 aaataattcg gatgagggaa cgactgcgcc aaatgcggca attcccgcag cggctgccac
    12301 gctacatgac acaatcgtcg gcaaatgatt caatgggtct ccaagccgct attttctcac
    12361 gggatagccc accatgccac agaagggtaa ccatttgcag gataatgccg tctaacctgg
    12421 aacgtaataa aatcaatctg gaaactgttt cttgcagggg ttgcgataat tggaactggt
    12481 tgagccataa agcggtatgt tcatggtagg tcgaagagga gtgaaagttg ccgggcaaaa
    12541 aaatcgcaat gctgttcctg ggtgtgttgg cattgcttgc atgcgtttgc cctcccttgg
    12601 ctgcgcagga caacccgctt tctaccgggg aacttgcgga ttcgcaactc tcggggattg
    12661 tgcatgcggc tgacggcacg gccattcctg gctcgacgtt gcgcgttaca cagacctcta
    12721 cgggaaaagc gtgggtaaca tggtcagacg aagacgggaa atttgagttc cccgccctgc
    12781 ccgagggcca ctttcgcatc gaagcttcac agcttggttt cgcgcccgtc actcaagagc
    12841 ttgacctcgt atccggcacc aagacacctg ccgatttgaa aatgaatgtg gggcaattgt
    12901 cggctattac agcgccgcag gcgggaacca cggaaacagc aaccacgaat gctccgccca
    12961 aagacgaggg cgcgaagcca gcagcatctt caacgccgaa tgctggcagt gaaccaaacc
    13021 atgctcaaaa tccgcctgcg acttcggcga ataattccac gccttcttcc ccaggtggga
    13081 aggcgggcaa tgtcgctgga tcgcaacgca gcggtggaaa tggacaagga ggcggaaggc
    13141 gctcgtttca gcaactgggc ctgaacggca aaaatcaaag ctccctggaa accgcgcccg
    13201 acgatcaaaa cgctccggag gcaggtgggc aattggggca agcagcttcg gcagatgctg
    13261 ttcaaatgac gggaaccgtg gcgatgggac agattgcgcc ggcggcagga cagcaagccg
    13321 gagagaacgg gccggatact cgcacaggaa tggacggcga taataacagc ataccaggac
    13381 aatcttcgcc gggcggcggt ggcgggccag gtggagttgg cggcggacca caaggagggt
    13441 tcggcagggg gatgagaggt ggaggcggtc cgcgcggccc gcgcggcggc ccacagggag
    13501 ttgccgcatt gtggggcgcg caacgcgtga tgcgtcaacg catcaaccgc gttcattaca
    13561 gtttttatga cacctacggt gcgtcccctt tcaacgcgcg tccctactcg ctgtatcagg
    13621 cgaatccacc caaaatttcc agttggacgg agtccgccgg gttcaatatg ggcggaccgc
    13681 tgaagattcc gcacatttac gatggcacgg ataaaacatt tttctatatt aattttggtg
    13741 gaacatggtc gcgcactccg gtggatcaat tcgcgactgt gcccacggtc gcggagagaa
    13801 acggcgactt cagcgcggat aacgtgcagc tttataatcc gctgtcgaac ctgaatggcc
    13861 cgcgaggatt gatgaccaat acagggtgct atcaggctac ggcatcttct cctttgcctc
    13921 ccgcgggaac atgcattcca acgaacttga tcagttctca agcgatccaa ttgctcggct
    13981 acattcccct ccccaacatt cctgtgacaa actttgccgg gcaaactttt aattatcatt
    14041 tacagacgaa tgttcccggc atcagcaatc ggctgaatgt aaacgtcacg caccagatct
    14101 ccgcaaagct cagcctgcag gtaaattaca atttcagcga cgccacctcg cattccttaa
    14161 acgatttccc ggaaattgaa ggagatgtgt ttacccgcgg ccagagcgcg atgatcgcgc
    14221 taacgcaaaa ttggaccaag acgttcatgc acacgtccca attttatttt tcgcgcaatc
    14281 gctcacttgg cctcaacgga ttttccaatg ttacggatat cagctcgcaa atcggactca
    14341 acgggatctc gccttccgcg tctgacttcg gcctgccctc catcaacttt acaaatttct
    14401 cggggttgag cgatcccacc ttttatctga gtcgcagcca gacgtatcgc tatgtggaca
    14461 gccttcgctg gatgaaagcg aagcacacga tcacagttgg cggtgaagtc aggaaggtgg
    14521 acattaaccg cgacactgac ccggcaccca acggacaatt ctcttttacc ggcttgatga
    14581 ccagccaact gtctgcagcc gggacgcccg tgaactctcc agccaattgc cagacatcta
    14641 ccgcctcagg gccttgcaat ggaaacgatt tcgcggattt tctgctcgga tatccggcga
    14701 atacaaaggt gcaatacggc gacactgcta cgtactttcg caattggggt ttcattggat
    14761 atgccagcga tgatttccac gcgtttccaa aatttacggt gacgtatggc gtgcggtacg
    14821 aagcattcac tcctcccacc gaagtcaacg ggcacatcgc caacctcgcg gtgagcccgg
    14881 atttcacgca ggtgcaatgc gtcactccgg ttgcaactgg aaattgtatt gccggaccca
    14941 ctgcgtcgct gtttcatggc cattataata attgggcgcc ccgcattggc atcgcctggc
    15001 agccgccggg aaaatggttt tccggacagc accaattgac ctttcgcgcc ggcttcagca
    15061 tgttttacgt cgaagcctat ttgaatacat tgtccgccga aatggccaat cagccaccgt
    15121 tcgccactgc cagcacactc acgcagcgga atttcaccac tgctccattg agttttcaga
    15181 ccaatctctc caccgcattg ccggacagtg tgacgaacac ggttggcgta aatccgaact
    15241 atcaagttcc ctacgcgatg gtttggaacg cgagcgttga atataatttg gcgtccaacg
    15301 catttctcga agtgatgtat acagccacgc gcggcattca tctcgatgaa cttctcggat
    15361 tcagttcggt gacaagcgga acgacgaatg ccgcggggtt cacctacgat acgaccggtg
    15421 cgttctccaa tttccacgcg ttgcaagtgc gtgttcagaa acgtatgtcg cacggcatta
    15481 tggtcatggg gcgctacacg tattcaaaat cgcttgacga cgccagcacc atcgggggag
    15541 gcggcacaac ggtcattcaa aataacgccg atccgcgcgg cgattacggg ctgtcatcct
    15601 tcaacatgac tcatcagttc ctcggccagt atgtgtatca gtttcccttt ggagatcgcc
    15661 ggagattcgc tgtgaagggc tggcagaaac atgtgttcgg ctcatggtat accaccgcta
    15721 tatttaacgc tcatagcgga actccttaca cggttcgagt tttttcgaaa aatcaggcat
    15781 gccagaatgt gccggggaca aactccgagc gtgcggatca gacaggaatt gaatcgctgt
    15841 ccaatccgag cactctggaa tggtttaata ccagcgcgtt cagcattcct acggtctctt
    15901 gctttggcga tgcgccgcgc aattcagtga tcggcccggg tgctttctca attaacacgg
    15961 cgctgacgaa attgattccc tttggacgcg acggcttgcg gcggctggac ctcacctgga
    16021 gtgtggcgaa tgtcctcaat catccgaatt acacggggct gtcaaccgtt cttggatcca
    16081 ggaccttcgg acaaatcacg agcgttggtt ccatgcgggg aatgacattc tcgacgagga
    16141 ttaatttcta atgactgcgc gatggatctt cgctaaacgc atgattgctg cggcggcggc
    16201 tacatcgctg cttgcggcgc cgctcggagc gcagagttcg ccggcacaag cacagacttc
    16261 ccaaccacag ccgcagccac aaattcaaca aatccctgtc aatccgcgcc agggcccgac
    16321 tcctccgcct cctgcgaaca aaaccgctcc ggacggcaag gcgacgatcc gatcgacggt
    16381 cagtctcgtg gaaatcgatg tgcaagtaac gggccgcgac ggcaaaccgg taaaagggtt
    16441 gaagcaggac cagttcagca ttacggaaga cggcaagccg caaaaaatat cgacgttcga
    16501 gtataacgac atcgaacaga ttgagaccgc aggcagcgtc gatactgcgc cggtcacggt
    16561 gccgctcgga acggacagca cgccggaaga aattaagacc gttgtgcgcg atcaccgaat
    16621 gatcgtgctg ttctttgacc ttacttctct tcaagcggaa gacttgctgc ggtccacgcg
    16681 cgcagcccag aaatatttgc aggagcagat gacgcctgcg gatttagtgg gcgtcgtcgc
    16741 atttggcaac accctcaagg tcgtaacaaa ttttacgaac gaccgcgatc tgctgaagca
    16801 atccatcgat gcgcttgtcc ctgggcacga agccgcgctt gcagcattgg ccgatgcggc
    16861 cacggccgca aacggcgaaa ccgccgtcac cgaagatacc ggcgcggcgt tcaccgccga
    16921 tgatacggaa ttcaatattt tcaatacgga ccggaagctt gccgccgtcg aatccttgtg
    16981 cgaagtcctg gaaaacatcc cgggcaaaaa atcaattgtg caattcacga gcggcgttac
    17041 gcagacgggc gaggaaaatc gctccgaact gattgccgct acgaacgccg cgaaccgctc
    17101 gaacgtttcc atttattccg tcgactcgcg tggtctgctg acggcaactc ccggaggcga
    17161 tgccagtgcg ggcgcgtcgg gcggaaacgc gctttttacc ggggcgactg ttatttcgca
    17221 aagccaatcg cggcaggatt cgcgcgaaac tctggcgacg ctcgcgggag ataccggcgg
    17281 gcgatccttt tttgacgttg gcgatttcgg caaagtgttt cagagcgtgc aaaacgacac
    17341 gtccggctac tacctcgtgg gctactacag tacgaatgcc gcgacggacg gcgcgtggcg
    17401 ccgcattcat gtgaaggtcg atcaacttcc cgcaggctcg cgcatacgcg cccgtgaggg
    17461 gtattacgcg gcaaagaatt tcggcatctt caccacggaa gaccgggaac gccaactcga
    17521 aaatgcgttt cagtccggta atcccgaggt ggagttgccg gtggcggtgg aaacggcgga
    17581 tttccgcttg aatgcgaacc agatttttgt gcccatcgct gccaagcttg cgcccagcgc
    17641 tttgcaatgg gcgcagaaac gaggcagcca cgaaacttct tttgatttcg cggcggaagt
    17701 gcgcgatgcc aaatcgaatc gcgtggtggg cgctctgcgc gacacggtca gcgtgaagat
    17761 cgatgcggaa cattttcagg acatgcagca gcgtgccctt ctctaccagg gcggtatcat
    17821 tcttgcgcca ggcgaataca aacttaaatt tttggcgcgc gaaaatgaat ccgggaagat
    17881 cggcacattc gagcaaaaac tttcgcttgc ggcgccgcaa ccggatcgtc tgcaattaag
    17941 ttcgctgctg ctttccagcc aggtacaagc cttgcagaac atggcgcaga ttaaaacgca
    18001 ggcgctggca caggacgcca aaatgaaatc gtcgccgttg gacgttggcg gcgagcgcat
    18061 tattccaagt gtcacgcgcg tttttaccgg cgaacaaacg ctctacattt ttttccaggc
    18121 ttatgtgccg caaaaagcgg actcaaattc gattcgcgcg ggcctcgtat ttttccgcaa
    18181 cgggctgcgg ctatccgata cgcctatgat cagcgcaacg gaattcgatg acaaaagtcg
    18241 taccgcatca ttccgcgtga gcattccgct cgcggatttg gcaacgggac ggtacaccgt
    18301 gcaggctgtc gtggtcgatg cgggaacgac gtatactgcg tttgcccgca attacttcgc
    18361 gctccgcgcg gctcccaaac ctgctgcctc tgcgatcgtt ccgcccgtgc ccggtaataa
    18421 ctgattcaga ttttgccgcg acccatcgtt cctgtttccc acctacaagg ttccacggac
    18481 cgtgcatggc ttaaaaaacc tgcgatgcaa cattggccca taacaaagca tctctaagta
    18541 agtagtcgat tccacatttt tgccggaggt attgctggca ggtgtaccga aattcggaaa
    18601 gttcgcgggt cacaatttgg gcaagaagaa atttctatgc gccatgtcgc ctccattttc
    18661 attctgattt tatggtcctc cgtttttatg tatgcgcagg aggatcacgt gcgaggcgtg
    18721 aggtcaggcc ccgcgctacc tgaagtcatt gatctcaatg tgtcggcagg cactccgcta
    18781 cctgtgctcc tggataacga agtccgcatt aaaaaagcgg ggcaggaggt ccacggaaaa
    18841 atcgcggaac ccgtatacgt atatgacaaa ctggttgtgc ccaccggaag cgacgtcctt
    18901 ggaaaagtaa cccggattgg cggcgtaccg gctttgaagc gtacatacgc agcgctaaac
    18961 tcagattttt ctcccgtccg cgacgtggaa attacattca accgattaat tttgtctgac
    19021 gggagtgaga ttccgctgca tacgcgagtc tcacttgcgc ctcaaggagt tctgaatttc
    19081 actaccgcga cagaacccaa aaatggaagc aagagcaaca aacagaatgc cgccgttaaa
    19141 ctggcatctg aaaaagtgag cgagaaaaaa caggaagtga atcgcgaatg gaatctggca
    19201 aaggaacaga tcaccgcgcc gggaaaaatt caccggttga aaagaatatt gatctcggaa
    19261 ctgcccgtcc acccgcagta catcgaggca gggacacgct tcaacgcgga gcttatcgat
    19321 cccttgcatt tcggaacaga aagtaaatcg gcggaagaat tgcgaatgat tgggactacc
    19381 ccagcgcctg gaagcatcgt tcatgcaagg cttgaaacgc cgctaagctc cgagaccgcg
    19441 caaaaaagtg aatcgataga agccgtactg accgcgccgc tcatcaccga caatcaactc
    19501 gttcttccgg agggaacgct gctgaaagga agtgttttgc aggttcgacc ggcgcgcaga
    19561 ttgcaccgca acgggcagct gcgaattatt ttccgtgaaa tcgtgccgcc caattccgca
    19621 cagcagaaaa tcgaaggcag cctcgaaggg gtcgaagcga gcaacagcgc agacttaagt
    19681 ttggattccg aaggcggcgc tcaggcaacc actccgaaga cgagatatct gactacagga
    19741 cttaccttgg cgctcgccgc cgcatcttta gctccggacc gggatgccgg aagcaatgga
    19801 ctttccgttc tcgaagttgg caggcaggga gcgactggcg cgtcaggatt ccgggttgtt
    19861 ggatttacga ttggattgct tgttcattca aggcctctcg cagccggttt tggcgcctac
    19921 ggcgctgcga gatcgatcta cagtaatttt ctttcgcgcg ggcaagaagt cgtgtatccc
    19981 aaggattcgg tcatggccat tggatttggg tcgcgcgttg caacaaccca aaaaacctct
    20041 cctccgcact aaatccggcg acacagttac actcccccca aaatggattg aattggcaac
    20101 gttcatgctc ctactcctct tgaggaactg aaaaagagga aagctgcaaa tgtttgaacg
    20161 gatcgttttt attcaaaatt cgcgctcaat tcttttccat gcgactgatt ctttctaatt
    20221 tttggatttg atgcgcaacc ttcgaacaaa aatcaaacaa aaccgtgagt tcgtctgcgg
    20281 gattgagttc cgttttggtc ttcagaattt ccaatttggg agtgaataat ggattcggtc
    20341 ctggccgtct tactacatct gaaatcgatg ttccgcctgt ctaacttact tctcatcttt
    20401 ccggtcggaa cagcgtttct tattgtgctg ctcatggcga ggcgtgcgtg gagagtgtat
    20461 cgcgaacagc gctatgacgc cgtttcgttc aagatccatg gtcagtggcg aaatatcgtc
    20521 cgtggcgaaa taccgccaca cgagtggcgg aacaactccc taaaaattga aattctgcaa
    20581 tcgattgtaa tcggtgaaat aaacggtgtg acggacaagg accgcgccgg attgcaaaag
    20641 ttcttgaggg agagcggctt gctcagccgc tgcatcgaac gggtactcaa tgaaagtgga
    20701 tgggcgaaac gccacgcgat gctggatttg ggctcgatgc gcgttccgga gggaatcgat
    20761 tccctcgtgg aaacattgga tgactggcaa ctggacacgc ggatcactgc cgttagagcc
    20821 ttgggcctaa ccggcttgcc cgaggcagcg ggaccgattc tggaagctca tatggttggc
    20881 ggcctcaaag ttcctaccgc gccgattgcg gacgcgctga tgcgttgctt caacgatcgt
    20941 ccggaagcct tactgcccta tttgcgccgc tcgatgggag aatcacgtgt attgctggcg
    21001 cgagtcgccg gagaattgtc cacgccgcaa atggcggacg aaataataat tttggctgag
    21061 gatccggaac cagaaattcg agccagtgcc gcgagagcct tggccgcttt gcctctcccc
    21121 ctggcgattc ccgtgcttgc caagctgatt cgcgatgatg cctggttcgt tcggctgcgt
    21181 gctacgtcgt ctctcaatca gattcttcac cctcgcgtga ttcctattct tctcgaagcg
    21241 attcgcgacc cgcaccggct ggtccgaatt cgagccgcct cggcccttgc caagttcgaa
    21301 cacgaaacca tggatatcct gcagagcgtc gtggattcgc gcgacaaata cgcgctgcac
    21361 gccatgattt ccgctctcga ccttggcggc ggatttggaa atgttttggc tctgcttgcg
    21421 gacccggaac aacacaacga agcggctgct agtttgctcg acgcgctgcg taaaggggcg
    21481 gctggtcttt ggactatgaa gcccgccgat cccgcggtag aggctgtctt tccctagatg
    21541 aaatgtggcg cgggttttag agtctgtgtg agaactgcaa aattaaagct tcagcggcta
    21601 aagccgcgct cattttgcag gacttatgtc gtggctaaag ccacgaccca caaagattct
    21661 gcagttctcc cacagacgct ttagcctgcg ggtttgagtc cttgtttgga ccaacttcga
    21721 caggctgaag tccgcgctac taaattccta atcccaaggc aatccgacat aattttctgc
    21781 taaggtcgtc atcgcggcgc gcgaacttgt tacgtaatcc agttccgcga tctggcggcg
    21841 cccgtcgaag gcgttatcgg tatcaaaacg gtggagcatg gacgtcatcc accacgagaa
    21901 acgctgcgct ttccacacac ggcgaagaac aatctcagaa taccgctgca acggctcggt
    21961 ttttccagtc ttataaaatt ccgaaaaggc ctgcgccata atgcgcacgt cggccacggc
    22021 aagattaagg cccttggcgc cggttggcgg gacgatgtgc gcggcgtcgc cggccagaaa
    22081 cattttgccg tactgcatcg gttcgactac ataacttctc attcccgtaa tccccttctg
    22141 aaaaattgct ccctcgttga gtttccagtt gccgtccgct gcagttcgtg cgcgcagttc
    22201 gctccagatg cgatcgtcgg accatgcact gatgtcttca tcgtgcggaa cttgaagata
    22261 cagacggcta acttctgtcg tacgcatggt cagcaaagcg aagccacgtt cgtggtagtt
    22321 gtaaaccaat tcaggtgacg aaggtggggc ttgcgcgaga atacccaacc agccgaacgg
    22381 atagatgcgc tcgaattcag tcaatacgcc agcaggaatg ctaggtctgc agatgccatg
    22441 gaaaccgtcg caacctgcaa tgtaatcgca gtgaagttcc tgttcctcgc cgccgacgcg
    22501 aaagcggatg gatggatttg cgccatcgaa attatgaacg ctgacgtcgc tcgcctcaaa
    22561 aattaacgga aggcccgctt cgaggcgcgc ggctacgaga tctttaatta cttcttgctg
    22621 cgcgtagatc gtgacggttt tgccgccggt caaatcgtta aagtcaatgc ggtggccgcg
    22681 tcggttaaat cgcaattcaa ttccgtgatg cgtgagagct tcgcgttgaa gccgcttccc
    22741 cacgccggtt tcaatcagca gatcgactgt gccctgttcc aggagccctg cgcggattcg
    22801 ctcttcaata tattttctgc tacgcgattc gatgacgacg gattcgattc cttgcaggtg
    22861 gagcagatga gcgagcacca atcccgcagg ccccgcccca acaataccaa tttgcgtgcg
    22921 cacggtttcc ccttcgtcgt aggcgatgtg tatcggaact acgcgcagtt tagacacacc
    22981 aggcacacct tgtccacgaa acgaatgggc aagaaatttt tttgcaaaaa tttaagggaa
    23041 aagattaatc gttggagatc ggccgaagga ccgcgacggc gaaatgcgcc agtagtggcg
    23101 cgccctcgtc caattgaagc cagcgtacac cgcaatgctc cttcaagtat ttgccgacac
    23161 gcgcttcgtt gatgccgaga tgttccgcga gtttttcttt caggttggaa ccttcacgcg
    23221 taccgcgccc gctgcgcagc atgtttccga tgccaacgcg caacgtctgg caagcttcgg
    23281 cgtaatagta agtggtctga tccgtgtccg taatcaggaa aacgcccggc cctgcaggcg
    23341 catcgtcggc ctgttccagc gaacggatcg agcaggccag caaacggcgg agttgggatt
    23401 ccaattgcgc caagcgtgaa gaaagcgcgg gatccccgga cctgacacgc gcggaaagta
    23461 tgggaccggc atgagtagtc tcgtaactgc gcaccggctc cgcctcgcga actcgagact
    23521 cggcaatatc agccgacacc gaaggccgag cgacgggaaa cggccgtgac tccgcggcca
    23581 cacgatcaaa catggagggc ggagctggcg gggcagccgg aactgccgct gccgccgacg
    23641 cggaagccaa cgtcgatggg gctgcggtgc gcacgccttt gtgacgcccg cggccaccgc
    23701 ggcgtccgcg acgcctgcga cggcgatctg ggccgacttc cggaggctgc actgcgggcg
    23761 cgaccgcgcc ttcccctgtg ggctgttcga aaacagtttc ttgagttcct tcggtcattt
    23821 cctgctcggt ttcgttcgaa tcttcttccg aaaattctga aataattggt tccaactgcg
    23881 ccaccgtttc actagtagtt tcgctgacag ccactcctgt aggaatggca ggcgccaccg
    23941 gtacaggcgc gcccaggcga actaaacctt cttcagcttt ttgtttccat tgtgggcgac
    24001 ggaaacgttc ggcagccgtt gtaaaccatc gcactgcctc agaaattgtt ccagccttct
    24061 catgaagctg ggcaagttcg aacgctacca tagcgtcatg ggttcgttca tataatgcgg
    24121 acagttggcc gaggggatcc gagctggtcc ttgccttgcg gatacgggcc tgaatttgtc
    24181 gccaatcgct cactgatcaa cattgtaaca gtcaagcgga cgctgagcca tagcaactga
    24241 tagaaatgct atgggagtgt gctgcatctg gttgccggtt gcgggaactg cgcgatatca
    24301 tcgagccttg accctctcgc ccgccacact gagcgaattc acccggctcg cagcctacgc
    24361 gattttcctg ttgagctatt tggtatttgc aatcggaaaa ttcccgggaa tgaaaattga
    24421 ccgcccggga gctgcgatca tcggggccgt gttgatggtt gcctttcgcg ccgtccagcc
    24481 tgcggactct ctgcacttca tcgatttcgg gacaattgtg ctgttatttt cgatgatgtt
    24541 gctggttgcc tatcttcacc tcgatggatt tttcgattgg gtctcggaat ggatcgcggc
    24601 gcgaattagt ccccgacatt tattgccggc aatcattttt ttgagtggga cgttttcagc
    24661 gttttttgtt aacgacatca tttgcctgat tatggttccg tttacgctga aggtttccag
    24721 gcggatggga ttgccacctc tcgcgtatct cctcgccgta gctacagctt ccaacatcgg
    24781 cagcgtcgcc accatcacag gcaatcccca aaacatgcta attggttcgt attccggaat
    24841 gcgctacagc aggtttttat ttcaccttgg cccggttgca ttcttcgggc tgttagtcga
    24901 ttggctagtc ttgtggttca tttacacacg tcacgaaact cctgaggcgc ggcctgtcga
    24961 tgttgccgtt tctgcatccg gctcgcatgg cactttgatg aaagctggaa tcgtaatttt
    25021 gcttgtcctg gcgggatttc ttgcgggatt gccgcccgca caagttgcgg cagtcggcgc
    25081 agccatcatg ttgatgacgc gaagacacga cccgaggcaa gtatacgatg aagtggattg
    25141 gggcttgctt gtcttcttcg taggtttgtt cctgattgtg ggtggcgcgg aaaatgtggg
    25201 tttgacggaa cgcatcgtag aagtgggcag gcgatggaat ttgcagcatg cgtatacact
    25261 aacgatcgtc acggcgggac tgagcaatat tgtgagtaac gtgcccgctg tgatgttgct
    25321 gaaatcggtg gtgccgcgat ttgcgaatcc gcagcaaggc tggctcgttc tggcgatggc
    25381 cagcacgctg gcaggcaatc tgacgattac cggttcagtg gcgaatatta tcgttgtgga
    25441 gcgcgccaag cctgaagtga agattacgtt tgggcaatat ttgcgcgcgg gcgttccaat
    25501 taccctgatt acgctggcgt ttggatggct gtggctgatg tgggcaagtt gaaccgcctg
    25561 ttcaggaaat aagaagccat ccccataact ggcatgtctt ccaaactcgg cagcgcgaca
    25621 gggttaagcc catgtttttt taaagttcct gaatgtcgcg gctgaagccg cgactcacaa
    25681 aggccattta tgagatggct tgaagtgttc tcagcctgca atcttattta atcggcgctg
    25741 accataaccg aaagcatttc ctgctgcgca gaatgaaagg catcttgcaa ttccttggcg
    25801 gttgaaaaca attttccatt ggcatgccgc gcgggaaatt gaaaagtaat ggtgcgctcc
    25861 gcgaacggat acggcttcaa aataaagcgg cggactccca ccgagcgaac ttcgacaggg
    25921 acgcgcctgc catcgcgcgt cggcaaaccg tgtaataaat tggccggatt tttaaagtcc
    25981 acgcaggtga gcagcgagag attgtcattg gcttgcagca gatgaaagtg atcgagaatc
    26041 gcatcgccgc ttttatcttc cggcttcaac tttggattcg cagcgatttg ttggcgcaag
    26101 gaatgttgca gctcccgctg atcctcgagg aatcgatcca gcagaggcaa ttgcgacggc
    26161 gcaatcgttg agcggtctgc atggtcgtgc agcaagctat aggtgtgcat ggaaataaga
    26221 gtcgcggcgt aaggatcttc agcggcgatc aagcggactg cgcgatcacg caccgccaga
    26281 taatccgcaa gatcgatttc ctcgaatgcc gaatatttgc cgacaagttc cgttgaaaat
    26341 gcgctgggct tcccctgccg agtaatttgc ggcgctgcat cgcgtgttgc ccaaccgtca
    26401 tcgtgacgcg cgataccgcg cataacgtgt tcgcgcggct cgggaggcag aaataaatca
    26461 ttgccccatc gctcggcaaa cgctcccgcc aaacgagcat gatcgggatg cgtgacaagc
    26521 caccatccag tttcggtttc gagtcgcagc aattgttatc tcccccacga ttttattctt
    26581 cgctttacgt tttcattcta tttcagcggc gcgagccgat tcgtgccact tgtgcagaaa
    26641 ataccattcc agaaacatga ttagaaaaag aaaaccgaag cccagaacgg tcgcggccag
    26701 caccagggca aataaatatt cagtctggag ttgcgatgaa gcgtaaagaa tggaatatcc
    26761 aagcccgccc tctcccacgc ggccggaacc tgcaaacaat tctccggtaa tcgcgccaat
    26821 caccgcaatt ccactggcga ttcgcatccc cacgaaaaga tggggtatgg cgtttgggag
    26881 gcgcaatttg aaaagaagct gccggctgga cgcattgtgc atcaggaata ggtgtacaag
    26941 attttcatcc acgctgatca ggccctgcgt ggtgttcgca ataatcggcg cgagtgaaat
    27001 gataaatgcg atgagagtca cggcaagcat gcccgcgccc atccacatca ggatgagagg
    27061 cgcaatggca atgatgggca cggtttgcag cagtaacgta tagggataga gcatttttct
    27121 tacccaaggc gattgcgcaa agacgagcgc cacaagcact cccacaatga tgctcgctat
    27181 cagcccgccg gcagattctt ccgctgtaat ggccagcgaa ctcatcagag agggaaagcg
    27241 ggcaaaaacg gagagcgcca ccgcgtaggg cgaaggcaac atgaagcgcg gcacgcggaa
    27301 cacccacagg atgagctgcc acaaaaggag aaacgccacg aagacagcga ccgcgtttaa
    27361 aatcgtgatg gttctttgcg tcattgcagg attgtctccc gcaaagtgtg cgtaacgcgc
    27421 gaggccaacg catcgaattc tttggaaccg cggacatcgg aagtgcgcgg cattggcaag
    27481 tccacgcgga agtcgccgta cacgcgcccc ggattcggag caagcaccac aatgcggtcg
    27541 gaaagaaata cagcttccgg gacggaatgc gtcacgaaaa ccgcggtcca ttgctgctcg
    27601 gtgcgcaggc gcaggatttc ttcgttcagg cgatcgcgcg tcatttcatc gagcgcggca
    27661 aatggctcat ccagaagcat cacacgagga tgctttgcca aagcgcgcgc gatcgatacg
    27721 cgcattttca ttccaccgga caattcgcgc gggtaggatt gcgatacttg cctgagtccg
    27781 acaaggtcta ataaagaagc ggtttctttt tcacgccggt ccttcggcac gcgctcaagt
    27841 tccattccga gagcgatatt ttcattcacc gtgcgccaag gcagcagcgt cgcgtcctga
    27901 aaaacaaagg agactgtttc ccgggcattc tgcggcgtca ttccgtccac gcgtatcgtt
    27961 ccgcttgtag gcggggaaag cccggcgatc aatttgagga ttgtggattt tccgcaaccg
    28021 gacgggccga tgatactgac gaattcctgc ttttcaacaa ccaggttgat cgaatctaaa
    28081 acggcgcgag catctccgta tcgcttcgta acaccggcta tctcgatttc ggggacgcca
    28141 acgttcgcgt tcattgcggt tcctcagtta caggcaaata aaaagcagaa gcgtattccc
    28201 tggtatgaaa aatggaatgt gtcgagcgcc gccaatccca tgaggtagct gcgcaggaag
    28261 gaacatcgct gccgggattt ctgtccaaaa gcggaaacgc gctgccggcg atttcaagac
    28321 ggatgcgatc gcccttttca aagcggcagg aagtcggctc caaagaaaac tcccaacgat
    28381 gaattttgtc cgcctcatag ttcgtttcag caaaaagatg cgtggatcgc gcaactccaa
    28441 tgcaaataaa ttcagccgcg tcattgggcc tcacgcgcac tagcttggca gtgacatccg
    28501 cattcttcag ggaagtggcg cagtaaagaa caatccgcgg tttaccaaaa atccgcaatg
    28561 atttttcaag cggtccggtt gaataaacga gaagattgtt tcccatttca agcgcggcct
    28621 gatcgaattg gccgcttgct gccgccggac ctccaggcgc ggtgacgggc acttccggat
    28681 cgtgcacaaa aatatcgcac ggctcattcg ccgaccggct cgaaacagag agcgtgccat
    28741 ttccttttcg cgaattggct ctcccttcgc tacgtaaaaa taagatgtgt tccgcatctg
    28801 aaggaaactt ctccgcgtct ctccagcgat tttcgccgag cacgaagtgg cgtatgcgcg
    28861 gatctttgtc aaacgagccg gaatctttta gccaatgatc gaaccatcgc agcagaatcg
    28921 cgtccgtatc gatgagcgcc tctgggccaa agtccgcaga gccaatccgg tcgccccacg
    28981 gaatatgcac ccagggccca gcgagcaggt attgatgctg ccgcgcaaat tcgctgcccg
    29041 cagacttgct taatgccaga aaaccatcga cactgccgcg aagaaacgtg tcataccagc
    29101 cggaaatgtg cagcgcggga atgcagattt tttcaggatg acgactcaca tcgagcgcgg
    29161 cccagtaatc gtcaggcgta tcgtggtcga accaatcgag cacgtactgc ggcaaacctt
    29221 cgccgcgcag cgcaggatgc gcgcgaattg acaatacgct tgtctgcgat gcgagttggg
    29281 cccaggcctg ttcgagccta tcgctcggtt cgcgcagctt tttccgccgc gcatcttctt
    29341 tcagcagctg taatccccag cccaacgtgg aagcaagacg cagcaccccg ccctgataaa
    29401 accatccgtt atagagatca caagccgtca tgccgggaga aatacactgc aagccttcgg
    29461 gtcgctcggc tgcggccagt aattgagtca tgccctgata agaaaaaccg tacatgccga
    29521 cgcggccatt cgattcaggc cgcgttcgca gccatgaaat cgtatcggcg ccgtcagggc
    29581 cttcatttcg aaagggataa aactcgcctt cggaattgcc gcgcccgcga atatcctgga
    29641 tgacgacgtt gtatccgtgg cgggcaaacc aaattggatg tgcgtacaca acagtggagg
    29701 caatgtcgcg tccgtagggt tgacgcatca agagcgtagg aagcgcaccg gactgaggag
    29761 gatagtagtg atcggaaatg agttccaggc catcgcgcat acgggcgcac acgccacgtt
    29821 cgaggcggac gccttcgccg caatcaaccc atttcggtgg gctcatgacg tggacaggtc
    29881 cgtgtagtgg tacatttcgg tgagcgcagc ggccaaacgc gtggccgcct gttccaacca
    29941 gcgagcacct ttttccgaag tggccgggcg tggatcgccc attacgccgc tcgcagcgat
    30001 atccttggtg agccaggcaa aagttgcggg agcgtcctcc ggcagcaaca acgcgggttt
    30061 cgtaacatcc gcaatatagt tggatgtgta tgcggattgg tccacgagtt ccggcacgct
    30121 cgcgagaaga aaggaagttt cgacttcgcc ggcgtgaaat ccgtatgcgc gttcctgagg
    30181 attgagccct tcgaacgaaa ttccaccgga cccgtgcata gcgaacattt gcaggccaaa
    30241 ttccgcgcgc agatcgcggg ccattacatc gatcagcgca gtgttgccgc cgtgcgtgtt
    30301 gtagagaacg agctttcgaa agcccgcctt tgacaaactc gaggcaagat cgcgcaatac
    30361 cgccatgaat gtggaagagc ttaccgagag cgttcccgga aaaccaatgt gctcattact
    30421 cttgccgtag tggacaggcg gcaacgcgta cactggcagc tccgcgggca ggttgcgcag
    30481 cgcgtgcccg agcagcaaat tgttgatcag cgtatcggtc gcaatcggaa ggtggtgccc
    30541 gtgctgctca atcgccgccg tcggaagcac aagtagagtg gtgtcacgcg ggagcgcgtc
    30601 aacctgtttc caattcaagt aagcgaaatt tcgcccatcc ggtatccagg ttttcatttc
    30661 gttatcgctc cagccttcgt ggattccgaa tgaaaggtga ccatcttccc cggattgagc
    30721 aaacctttgg gatcgtagcg caactttgcg agtagttgca cgttgtccga gcgataacgg
    30781 ccgcctccct ccacattgtt tacgtgcggg ttggcgacga acactccaat tttgcggcag
    30841 aaatcgatca tgtcgttcag gcgctcctcc gatttgaaat acacgacggg gattgcgccg
    30901 ggaaccacgc ggccctcgcc atttttcata aactccatgt ggaacagaat ttcctggccg
    30961 aagcgttcgc gcaattgctt catctgatcg cgcacagtat cgggattaaa gccgcattgc
    31021 aagtaggtgt aggcgtcatc cgacctcatg gcccaaagag tagtgtgatt ccaggtataa
    31081 tccgaaagca gcggccgcgc attcaggccc ggataaattc cgctttgcgt gacgcttcct
    31141 cctgtatctc tcgcggcgat ttccaattca cctagctggc tggacgcaat catgaaaaaa
    31201 ataagcgtct tgcctgccgg cgcatattgt cgaatcgggg tgaagaaaga tggaattggc
    31261 cactcgaata ctgtcaccag ccgttttatc cacgcagagg ctgtggcaat cttctcggca
    31321 aaatcgaaag cggcgctgaa cgtgtcaaac gccgcgatgc actgcgacca ttccactgca
    31381 ggagtcagtg ccagccaaat gtgcgtgatg atgccgttgg tgccccaggc atgcaaaatt
    31441 tcgtgaatgg cttcgccttc gtgcaaaacg acgcggggct caggctccat ggtcacaact
    31501 tcaagtgcgc gcacagtctg aaaatcgcga aggccgccgt gcgcaaccgt gccgattcct
    31561 ccagaaccgc cgctgacaaa tccgccgacg gacgccttga taatggtcga aggatagcag
    31621 cgcaattccc agccggactt gcgcgcttca ttttcgagat cgcccaggcg gactccgggc
    31681 tggcagatag ctacaccgtc tgtctgaatt tgctcgatgt gatccatgcg gcttaagtcg
    31741 aggacaacac cgccttcgag cggaacagcc tgaccgtagt tacctgttcc cgcgccgcgc
    31801 gccgtaatcg gaatatggtt ggagtagcag tagcgcaaaa gtttttgaat ttcttcagcg
    31861 ctccgaggct gcacgacaac ttctccaatt tttccgtcga gttgtttgcg taataccggc
    31921 gaataccagt agaaatcctt ggaaagctgc tcgacgatct gcggatttaa aatgactcgc
    31981 gcgggatccg taataagtaa acgaagctca gagatatgtt tcggctccat cgatttcggc
    32041 cttcaatcaa atttgagact tggcagactt tgttttgctt gagctccaag tccggaacgc
    32101 ctttcgcata ttcggatgcg taaaaagcga attgcacaat tcacgcagca ctggcccatc
    32161 gacgatgcag ggcatatccg agcggctcga cacttctttc gcgggaatat gaatttgcag
    32221 acaaaagcga ttggcgtcgt caatcgtcgc tccaaacacg acgcgatcaa gcccggccca
    32281 caacgcattg gccatgcaca tcgcgcatgg ttcgcaggtg gagtacatcg tatagcccgc
    32341 aagagatggc cgtttcagtt tcttgcaggc gagccggacc gtgcgtgttt cggcgtgggc
    32401 gctcggatcg ctttcgcgga aaacggcgtt ggtggcgcgc atcaggcgtt caccagtgcg
    32461 cgtgtttacg atgagggcgc cgaacggtac cgggcgagtg gttatcagcg agcgcgccgt
    32521 gaaacgatat atctcacgca tgcgctcttc atccacggcc gcgccgcgcg atcttaaaat
    32581 ctctgtgaaa gccatgccgc aatttaccac aatgcgagca tggcctgatg gggacgtcta
    32641 ttcaattttt gcataggaac gattagaccg tttttagcta tttgcaccgt gatattctcc
    32701 acacagtgaa taacgatgga ctgattacca tggccaaacg aaatttcacc atcttgtgcg
    32761 ccgttacctt cgttgggctt ttcatatccg gatgccatca tcccgctgcg gataacggcc
    32821 tgaccaaagt agtgctgcaa gcagactggt atccgcaacc cgagcacgga ggctttttca
    32881 ccgcactaac caagggtttc tataaggacg aggggctgga cgtaaccatt ctcccaggcg
    32941 gtccatacat cacggtggag aagcaggttg ccgctggtgc ggcgcaattt ggaatggggg
    33001 cgtcggacaa agtcctggaa gcgagtgccg aaggacaatc tctcatcgcg gttgcggcta
    33061 cgcttcagca tgacccgcaa ggaattatgg tcaggaaaga ttcgcccatc cattccttcg
    33121 ccgatttaaa tggacattcc gtcgcgataa aagtgggcga atcggcatgg tttgaatatc
    33181 tgaccaagcg ctatcaactg aataacgttc gtgtggttcc ggcgatgatg aatgtggcca
    33241 attttgttgc ggacccgcag tatatccagc aggccttcgc gacgtcggaa ccgtttttcg
    33301 cgcatcaagc cggcattgag acgcgagtcc ttctggtcag cgatgcggga ttcaatcctt
    33361 accgcgtgat gtacacgaca aaggattttg ttcagcggca tcctgaaatt gtcgcgaagt
    33421 ttgttcacgc ttccgtgaag ggatggaagg aatatctcaa tgacccggtt ccagccaacg
    33481 ctctgattgc caaattaaat ccggctctca atccggagtg gatgcaattt acgtggcaac
    33541 aattgaagga tggtcatttt gtggcgggag aggatccaac cggagcacag atcggccaaa
    33601 tggatcccaa acgctgggct acgatgtatg aacagctcgt tgatttgaaa atgattgcaa
    33661 aaccgatcga tccaacgacg gcatacacct tgcaatttct cgggcctcag tagaaatcat
    33721 cagcagaaac aacagatgcg cggcgtcagt ttatgacgag ccgcgcttct gtcttaccta
    33781 ttaagtctac gtgcttattt tccgaaaacg agcactctga agtttccggc cttgggcgtt
    33841 gccagcggat gcggctgctt ttccgttggc gcggccgggg gattgaaatc cgaagtggac
    33901 agaaaaactt tgtgcgtctt tgaatccacc tgcaaagtct tggcgccgta ttccgtcttc
    33961 acggcttcca cttcgctcaa cttttcgggc gaatcttcgt gataaatgtg catcatgcct
    34021 tcacgcgtcg cgacgaatac cagctttgtg ccaggatcaa acgcgttggc atccactccc
    34081 gccgaaatcg gataggactg gatcaccttg cccgtatcgg cgttcatcat gatcatgaat
    34141 tgcggcccgc gcccgccgga gaaaagccgc cgcgtctttt ggtcaatcgc cagggaggtc
    34201 ggatttcctt ccggtgcggt cggatagcgt gccttgatcg tattcgtttt ggcgtcgatg
    34261 acaacgatat cgttcttttc gggattattg tcaaacacca ggcccttgcc gtcgacggcc
    34321 gggaattcca ctttgccaac cagatcgatc gtcgtgatca cggattcttt cactgcgtcg
    34381 atgaccactg tattgtggct gtccccgtta aaagtaaaaa tgtgcttggt cacgggttcg
    34441 tagatgattg catccgtgtc cagctgaccg gtcttcactc tgccggttat tttcagagtc
    34501 ttcagatcga aaactacagc ttcctgaacc gtggcgcctt ccctcgagtc accgtcagtg
    34561 atgaaccctt tgcccaaatc cttggcgagg gcaattccgt ggctgcgtga caacccttcg
    34621 attttgccca ctaccgaata gtcatcagca ttcagcacca caacttccgt gccgtgggaa
    34681 acgtagacgc ggcgcgcatc cgcatcaacc gaaatatagt cgtaatactc gccgccgccc
    34741 ggcgccgcag gcaacgaaat ggtcttcagc aaatggtagc cggaatccgc gggtttcggt
    34801 tgtgcgtagg agcccgacaa gaaacatgct cccacaaacg caaggcttag acccgccgcg
    34861 aggcggccaa tcgtagagaa cttcatgtaa acgctccttt ttatttggtc taccaaaaga
    34921 ttcgaaaaac gaaagtaact acgccgcacg ttctgacgtc tagcgtcaga atagtcactg
    34981 accggcgttg gcgcgatttt ttagactcct cattatcgta gcgggactcg gttgcataca
    35041 tgtcattttg cgccacataa actctttcgg gtccgaaccg caaaactgca gcatcgaccc
    35101 tagagacatg gtgagcacca gcctacacga tgatcctgaa ttattcctga atgaatcgtt
    35161 cgggaaaaag gaaatgtaac gaaagtgtta agttccggga agcgaaggca atggagcgct
    35221 ttcccgcaat tgtcatgcgc gggtttggat gcggaaagca aattcgcaat gccgcgcaaa
    35281 atattcatgg ggccttgcga atttggaata tttgagagaa ccaacctctg ctgacgttcg
    35341 aaatcccgcg gcaattacgc ctcaaaatgt cttgtgaatt cgcatgtccg cggcataccc
    35401 cccatattgg tcgcgcaaca cggaaacaga caaacctttc ttcacctgat attgcacctg
    35461 gatggcttga ttttccgttg aggttacatc ggtgctgaag gtgagcagaa ttttcccggt
    35521 gaccctctgt tgaatggcaa cttgcgcgcc aggattcgac tggctgttcc ctgccagcgg
    35581 atcaatagtc agttgcgata ttcccgccag cttttccagc ctgccgctca gttggccggc
    35641 ggcgccttgc gccagcacgg attccgcgcc gagcgttgcg ggcgtgctcg ggctggttgc
    35701 ggcctgctcg gttgttttgc cgaacgccac caggttaatg atgtcggcag gcggcaacgg
    35761 aggatcggat gtgtagttgg tcctcaatcg gtccacagga ccaacgaagt tcagagtgat
    35821 gttgtactgc tgcacggtgg ttttcacata caaattcacg acgggcacag tccgcaccgg
    35881 gttggcaaat tcaatcgtgc cattttcaat ttcgtagcgc ttccccatga aaaacatttc
    35941 gccgcttgtc agattcgcgc gccccaatac aacgggagaa gccatcgttc cggacagagt
    36001 taagttggca gcgccgccca tgctcacttt actgctggcg agattaatat cctgggccgt
    36061 tgcaacggct acgttcaatt tcatattggt ttcaaaaggc gaagaagttg tggccggagt
    36121 atcgttggaa agctggccaa gaagcgtggc caaatcgaat tgctgcgtga aggaaagtcg
    36181 atcgatcaac acgcgaccgg tcagttgtga actcgcggga ttgccggcga gcgaaagatt
    36241 gccggtcaat acggaacgaa ttccctccgg atatcggatg cgcacatttt ttgcatcaag
    36301 gcccagatta aaattggatt gcttgccata tgtcataaag ccgcgcacgg ctacggagcc
    36361 tccacccgcg gctcctgaca actgtgcaac ttccagccga ttcccgttga tttgaatttc
    36421 tccgttcatc ccttcaatgc tcaccggtaa tgtttcggtg gaaaccgcgg cattcgcaat
    36481 ttcaaccttg ccctgcatgg caggattgga aaggtcgcca cgtgcggaca tatttaagtt
    36541 gatgtttcct gaacttttca ttccagttgt gaacgcttgc agaacggaca gatcgagcgc
    36601 tccgtttgct gaaacattca gcggcaccga cgctttcacc ggaattatgc cttttacatt
    36661 taaatccgtt ccagttccct ttagttccgc ttgatcgatg ttcacgatgc cgtttcgata
    36721 tgtggcgcga agcgggcgga caaggccaag cttcgcttgc tgatagttca catctaatgt
    36781 tggaatttca agctgcgcgg taatcgcatt gggatttttc agcggacctt ccatatttat
    36841 atgaatttcc gtttgtccct gaatttgctg cgttttattt aaataacgcg cgagaaccag
    36901 tgcgacaggg acggctcgaa tatcgaggga cgcggtggcc ggataatcac cggtcagtcc
    36961 aacatttcct tttgcctgaa tcgatccctg atccgccttg gaattcaacg tgaacgttgc
    37021 atgctgatcg gcaagactta actgcgcgtt aacatccgag accgtttgat ccctgattgc
    37081 aagttttgga atttgcaagt tcgctgcgaa ttctggattc attaaccttc cgcgaccttg
    37141 cgcggacaaa gttaaatcac cagcaacgcc gatgttgcgg gcctggacgg attcgagccg
    37201 ctccaatttc attcctgtag tatttagatt gatttcatac gattgcgttt taggctggta
    37261 gatcatggct gccgtcaaat tcccggcagg aatggtcaat tgaacttcgg aatgaatgga
    37321 attgccgtca ccgttgaaat tggcgttcaa attctgaatg ggttcgttcc aagcgcttgc
    37381 cctggtaatc tgcaacgtgc cttctccttg cggattttgc tctgagccat gaatagaaat
    37441 atttgccgca acggttcccg tcaccggata ttgcgcttgc aggaattgct gcaattctga
    37501 aatcgaaatc tgtctggcgt ttagctgcaa cgtaagcgga ctggtcggcg tgaaagacca
    37561 cgaggtcaaa tctgcgtgcc cgttgaaatt gatctgtcca ttttgtggat caatcagcga
    37621 tccattttga agagtgatgc tggacggggc ggcatccaga gtggcgtgaa aatttcgcca
    37681 gtgggcattc cgcacttgca gatcatttgc ggacaggtct cccttgattc gcggttgctt
    37741 aacgtctccc gatacctgcc cactaaagcg cgcagctcct ccaagggtgg gcaaggaact
    37801 tgccgcggca tttgcactag gggaactccc gctctgaatt gcagatacga gcgcagtcac
    37861 ttcatgcagg tctgtcgtat ttgcctgaac attcagctgc gaccggttgc ttaaaatgcc
    37921 cgaaatcgaa agtttcgtag tacctgtttg cagataagat tgcccgaaag aagcggtgtt
    37981 tcgcgcaccg tcataagcca catcaatcag gccgttcaat ggaatggtcc gatcccccac
    38041 agtgggtgga cgaggattac gaatatcaaa atgcacgcgc gccattgcgt tttgaacgct
    38101 ggaggtccaa gtcgcttccg ccgtcccgtt gacgcggccg ctgatttttg cagcttcacc
    38161 gtttcggatc gctgcagtcc tgtccatcgc ttgaagcgaa acgtcacgga caatggcacg
    38221 gagacgcgag ctcgaattgc cgctgatgcg tgataaatcg taatccgcgc tcacatgccc
    38281 gccgagcacg tcagcctccg catttttcac gctcaaattg ccgtcctgca atttaaattc
    38341 agcgcggatg gattttggat tcgtgcgcat ttgtccgatg tccaacacaa attcctcact
    38401 gttcaatcgc ccatccacgt acgcggtatc catcaccgac ttattcggga cattttcata
    38461 atgcaaattc cctgtcattg cgaccttacc tgctggaatc gatttgcttt ccgtgtatcg
    38521 agcaagctca atggtagaaa ccacgccgtc gtacgtgccg tcgattttcg gggaatcgta
    38581 attcgtaagc tttgcatgga ggttcagagt cgattgttcc gttgcaatcg taattgggtc
    38641 tgcggcgagg ccggaacggt ccagcgcgaa gtttaattga aggcgatgcg aaattggttt
    38701 tagtgtttcg gccatgatgc gtccatcgct gtacccgatc gtgccgcgat attcgctggt
    38761 taccgggttg aaatgaatgt ccgcgtgcaa atcgcggagg tcggcatcga gcggcaattg
    38821 cgcg
//
LOCUS       DASZMB010000157        15861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2627848, whole genome shotgun sequence.
ACCESSION   DASZMB010000157 DASZMB010000000
VERSION     DASZMB010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 15861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15861
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2627848"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VG272_07190"
     CDS             <1..829
                     /locus_tag="VG272_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13503.1"
                     /translation="ISSLFGAPAQSSQMKLETSAAFDHYVQITETKMKKDLDSNQFLV
                     VDQLPEPKRNEAYEQLRRGEVFIQETHTADGHDPIHIPGGLIHHWAGVVYVPKATIKE
                     VNSVLQDYENEPRIYKPEIRRARLIKQDGDESKVYLQFFSKSMVTVVLDAYFDVTFKM
                     IDKTRYESISHSTRITEISNPDGPDPRERQDGEDHGYLWRLNSYWRLQEKDGGVYLQN
                     ESITLTRSIPILLAWIANPLIKSLPRDVLAHMLNDTQIAVAKKAKNPMPAPKQITKN"
     gene            841..1692
                     /locus_tag="VG272_07195"
     CDS             841..1692
                     /locus_tag="VG272_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HWF13504.1"
                     /translation="MKQARTAKTGSTRRAFISKSGLLLAGTVLATSNAMGVEASSADF
                     SAAAPIPQQDVAALRADLVTANHILYDQGVVDGYGHVSARHPANPNHFLISHWLAPDM
                     VTDADILELDFDCKPVGGEKARLYSEVFIHGEIYRARPNVKSIVHTHAPAVILMGISG
                     ETLMPMYQMTGYMGTGVPLFDIRKAAGMTNMLISDVARAKALVQALADKPAVLMRGHG
                     GVVTGSSLAQAVGRSVYMRVNAELQVQVLGKKMTYLAPEEAQLASDNDYPKDWEMWKR
                     KVAGQRT"
     gene            complement(1773..3440)
                     /locus_tag="VG272_07200"
     CDS             complement(1773..3440)
                     /locus_tag="VG272_07200"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HWF13505.1"
                     /translation="MKKILNSAFLAFLFTFAVSSYASPGGDISGTVKADDGAPFRAAF
                     VRAQNVKTKMIMMVLSNNQGQFRTDSLPAGTYEVWATSTGFRSEPSRLTDIAVEDGKT
                     TNANFTMKKGAVQWSQLTKYQAGILLPDGKGKDVVLQECFNCHAMSRIGMMGRDREGW
                     LEAIEHMRQVGVAEISPNIAAQVSEYLGSVFGPDAETPSSPAQLPQYQKVKQEHDYFS
                     DDALTIVYVDYQLTGEPKDRPGSSKQDKDGNMWMEMGGGLSKLNPETGELHTWRLPDA
                     TRPFIHEILPTSDGYVWLTIEKQNALARFDTRTEKFEVFRQPYGGPPPVEMEPNAPWP
                     NLRYTQGDQTSDPRRHTAVADLQGNIWSSGRPLSKFDVETKKYTFFPEVPDTYGIAVD
                     KEGNVWFAEFNSKDHHSIGKVDVKTNKVTKWTPPNSGTRPRRLKIDSQGMIWFGDYFE
                     GNLTRFDPKTQQFTEFKLPGPMPTPYGIGVDHNDQLWYASMYTDVIGKLDPKTGKVIE
                     YPTPYGERGTRDMFEDAQGRIWYGAQPYGKVGYVHLRKAMEKAVAQR"
     gene            complement(3576..4721)
                     /locus_tag="VG272_07205"
     CDS             complement(3576..4721)
                     /locus_tag="VG272_07205"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HWF13506.1"
                     /translation="MAASFVITGILLDGLLVKLPRSATDKISSERVGVVHIHTRASDG
                     SGTVADVMTAAEQAHLSFVAITDHNIAMTQSDLDEDPPDLPIISGEELGTTAGHFLAL
                     GIPASWKEPKTKDDRELLAAAGAVGAFRVLAHPFHPHTPWTDWQTTDFDGMEIWNEDA
                     TWRQNNFFDLINALIMYGENNQLALVRLARTPEQNFAKWDELLQQRPLAGICAADTHA
                     RVRLAFGKDWKFPQYAPSLEVAREHLLMSPDSGGGNPALASASELIDALRHGHSYCGL
                     DSLYPSDGFSLRIVGANVSGGPGDILHSSENERLKISVPAGSSLPLIKVFRDGHEIDE
                     QETRSIDEPVSGPGRYRAEVFLRQPGFSGWRRWTLWIFTNPVYVTAP"
     gene            5029..5676
                     /locus_tag="VG272_07210"
     CDS             5029..5676
                     /locus_tag="VG272_07210"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HWF13507.1"
                     /translation="MISFFRMRKRAGLLSLLLTLFFAVFVAAAHAQSPDPAETADRLP
                     VRGTEIEIWTGGGADPIGANGVTQGNSQWTAGLRYGWILTDAHGPKFLRGRFEYAVDA
                     LPVVMVFQPGGLAYGFGFDPWIMKWNLEPHHRISPYIELGGGGVISTREIPRGESRFN
                     FTPTGALGVNILRGKYHWSVDFRYLHFSDAEITSFNPGTDTFGVRVAWGVYTPSH"
     gene            5901..6272
                     /locus_tag="VG272_07215"
     CDS             5901..6272
                     /locus_tag="VG272_07215"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWF13508.1"
                     /translation="MNIRMAVIVVSLFACLGATCVGEMRAQNKASATAPPAKLAEDAR
                     RGEGLFLQRCSICHLPRKLKFGSPPVIGPSLSGQFKDVGPEQMKVLRGFILKGGPDMP
                     GFQYGLEPNEVDDLIAYLKTL"
     gene            6303..8096
                     /locus_tag="VG272_07220"
     CDS             6303..8096
                     /locus_tag="VG272_07220"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HWF13509.1"
                     /translation="MPRKMAFAVVFAAVVSLGLLVGAQRAFSQFATSVETAVPNASLL
                     GFVHSSDGKNLEGIGVSARKNGETFTTTVYTDESGAFWFPAMATGQYKLWAQAVGFET
                     YKTDVTFSDVPVRNVSIRLAPLADFHKQLSGTEWAESLPEDTPDDRRMKTVFINNCSG
                     CHQVSFLLQNRFDAAGWAAVITLMEKMQSIGYAPADIAPNVVIHSYKPELASFLGRVR
                     GPANASLNWKLLPRPTGDAAKIVITEYDLARPDMPGWTMEHNGTDWSEGTPSRWDGRA
                     AHDVAVDRGGFVWFADDATPGRTLAKLDPSTGKITDYMLADQNNAAESSHALVFDKAG
                     NIWFANGTEGSPTKFDPETGKFFKYPRPKDFPFSGDFITMDTKGNVWSPHREGAYKLE
                     PLTGKYTNFAMGPGKANYDIAADNEEKVWISQPGGNDMEMIDSRSGKIERLDLSPVIS
                     RDYEVTAKDRELAAGLELTPNTATPLDKGPRRSAADRDGDFIWVCEFFADRLAKIDAR
                     TKKITEYPLPHRFSQPYSATVAKDHTVWITMLNSDRIAKFDPATEKFTEYVLPTRGTE
                     IRHIQVDGSTTPPTVWLPYDRTNKIARIQFR"
     gene            complement(8200..9162)
                     /locus_tag="VG272_07225"
     CDS             complement(8200..9162)
                     /locus_tag="VG272_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HWF13510.1"
                     /translation="MKAIVVHEFGGPEVLKLEEFPTPKAGPGQAVVRIHAGSVNPFDT
                     YMRNGTYAIKPPLPYIPGGEGAGVVHEVGAGVTKFKVGDRVYVGHPVTGTYAEYSLAL
                     ESQLHGLPDNISFNQGAGIYVACGTAYHALHHHAHTHASETLLVHGASGGVGIAAVQM
                     ARAMGLTVIGTAGTEKGLELVRREGAQYAFNHRAARYTDEIMTATNGRGVDVVIEMLA
                     NVNLSADLKMLARFGRVIVIGSRGEVTITPRDLMARASSVRGFVLWSISEADEADVHA
                     GLFAGLENGTIRPVVGKELPLGEAARAHKEILETGALGKTILIP"
     gene            9386..10420
                     /locus_tag="VG272_07230"
     CDS             9386..10420
                     /locus_tag="VG272_07230"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF13511.1"
                     /translation="MIIDCHGHYTSEPAAFIEFRKQQIDAFNKSSAAPALPQITDDQI
                     REKLEPAQLKFQRERGTDLTIFSPRASGMGHHIGDAKLSASWTQICNDMIHRIVNLYP
                     KNFIGVCQLPQSTGVRPETCIPELERCVKELGFVGCNLNPDPSGGSWKEPPLTDKWWY
                     PLYEKMVELEVPAMVHVTATCNPAFHTTGSHYINGDTTAFMQFLTSDLFKVFPTLKFI
                     IPHGGGAVPYHWGRYRGIAQDMKLPPLTELLLRNVYFDACVYHQPGIDCMTKVIPSDN
                     ILFASEMVGAVKGIDPETGNYFDDTRRYIDNSTILNASDKKKIYESNALKVYPRLAKQ
                     MQKQGIAALL"
     gene            10657..11856
                     /locus_tag="VG272_07235"
     CDS             10657..11856
                     /locus_tag="VG272_07235"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13512.1"
                     /translation="MGKIVAAAGVVHAPQLFVRPPSEDPAQLDADIAAMRLVGKDLDE
                     TKPDVAIVIGNDHMETFFLSSVPTFAIMSGERARAEFARKSYDLPVHQGLAEDLLDKL
                     VNDDFDMAYSQDAVLGHAFAAMYEWVIEGRNIPVVPIFVNAYLPPLPTARRCAQLGKA
                     IAKVIAARPEKVAVIASGGMSHFPGTWKYPAPDFDFDYWAIAQMEKGNHEALLNLTSQ
                     QLDVVGNTEMLSWMIMFGMIGDQPGELITYQPTWHHGHAVMRFLPDKRTGKLSDEGKK
                     GPAEYHFKGSEGYEFYKHPSPSAYKLNKILYDLRFKPSLRVRLINDIPGVASEYGLPL
                     DEAKVLETLQDEDIEKFRNGKVHPLVQAGAHPLGMWMSVIVFHAELRRLRAAAAKSDT
                     ETASATQ"
     gene            11993..13015
                     /locus_tag="VG272_07240"
     CDS             11993..13015
                     /locus_tag="VG272_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HWF13513.1"
                     /translation="MPKMRALQIPKAKEPFQLVEREIPAPATGAVRIKVQACGICHSD
                     EYAKEGSRPGITYPRVPGHEIAGVIDAIGDGVQGWEVGQRVGVGWHGGHCGYCNSCRR
                     GDFVTCSKGRQITGLTCDGGYTEYMLISSAALAAIPDQISATDAGPLLCAGVTTFNSL
                     RNSGARPGDLVAILGLGGLGHLGVQFAHKMGFETVAIARGKDKEAFAKKLGAQHYVDS
                     ESQNVPAELEQLGGARVILSTVTSGKAVNAALNGMGINGKTILVGNPDQPLEITGRLL
                     IAGRRSISGWPSGSPIDSQDTLKFCALTGIRPMTEIYPLERAPEAYDRMLSGKARFRV
                     VLSTGL"
     gene            13114..15108
                     /locus_tag="VG272_07245"
     CDS             13114..15108
                     /locus_tag="VG272_07245"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HWF13514.1"
                     /translation="MPQLHRMDRNSEQPYTLLVNLDRDLLTPLRRYWGYDAFRPLQER
                     IVRSLVDGRDTCVIMPTGGGKSLCYQLPAAMSENRTVIVISPLIALMEDQVAQLGQMG
                     IPAALLNSTIKPADQSRIIEKARAGGYRLLYLSPERLAREDTIEWLRGVPIMFFAIDE
                     AHCISEWGHEFRPDYRLLSRLRAHFPDRAIAAFTASATQRVRHDIIRQLELRNPDKYI
                     ASFHRPNLRYVVRACDAHSQTELLLRAMRSYGDENLIVYAPTIAKVESTVDFLQENGI
                     PAVAYHGQMDAADRRRNQERWMTDEVRVMVGTIAFGLGINKAAVRAVIHLSLPKSIEQ
                     YYQEAGRGGRDGQPADCVLLWQKRDVGLHAYFIGQMNDPQEKQRAWQRYDEIKGFVES
                     AHCRHQRICEHFGENKKWVACGACDICSYQPEWLIAPVQEAPRKSKRPRSRSTALTSA
                     PIARTWEKPAPRPAAERAPNAVEVDPNLREYMREWRKDIAKEQNVPAYVVMHDVTLDA
                     ICAVRPTSIARLLLITGIGERKAELYGSQILNALKQFEGGARATAAEKKITPSEETIQ
                     LIEGGKSLEEIAVIRGRRISTIVSMVSDLVERGLVEFHTEWVPENRRNQIESVAADLG
                     LDRVTPIKNELPEDFTYDEIRLVVSKLRRDSDSKPLGATT"
     gene            complement(15262..>15861)
                     /locus_tag="VG272_07250"
     CDS             complement(15262..>15861)
                     /locus_tag="VG272_07250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF13515.1"
                     /translation="KFLTDPVWWFYLFWLPTYLTDMRHFTPQQRGMALMIVYSISALG
                     ALMGGVVSSFLMKRGWTVGKARKMTLLFCAVVMPACTLGVVVQDARLAVLLFGLATAA
                     HQAWMTNLFIMPADVFPSRAVGSANGFGVCLGGLGGALFSGIIPGTVIPHVGYVPVLM
                     GMSCFYIVAWVIIHRMMGELEMVSLGTGNDPVASLELAS"
BASE COUNT         3745 a         4509 c         4210 g         3397 t
ORIGIN      
        1 catttcatct ctgttcggag ctccggctca atcatctcaa atgaagctgg aaacttcagc
       61 cgcctttgac cactacgtcc agatcaccga aacgaaaatg aagaaagact tggacagcaa
      121 tcagttcctg gtagtcgatc agttgcctga accgaagcgc aacgaggcct acgaacaact
      181 ccgtcgggga gaagtattta tacaggaaac gcatacggcg gacgggcatg atccaatcca
      241 cattccggga gggctgattc atcactgggc gggagtagtg tatgtgccga aggcgacgat
      301 aaaggaagta aattcggtgc tgcaggatta tgaaaatgag ccgagaattt ataagccgga
      361 gattcgtcga gcaaggttaa tcaagcaaga tggagacgaa tcgaaagtct acctccagtt
      421 tttcagcaag tcgatggtga ccgtcgtgct ggacgcgtat tttgacgtga ccttcaaaat
      481 gatcgacaag acgcgatacg aaagcatttc acattcgacg cgcattaccg aaatttcgaa
      541 tccggacgga ccggatccgc gcgagcgcca ggatggcgaa gatcacggat atttgtggcg
      601 cttgaacagc tactggcgat tgcaggagaa agacggtggc gtctacctgc agaacgaatc
      661 gatcaccctg acgcgctcca ttccgattct gctggcatgg atcgcaaacc cattgattaa
      721 gagcctgcct cgtgatgtac ttgcgcacat gctgaatgac acacagatcg ctgtggccaa
      781 gaaagcaaaa aatcccatgc cggctcctaa gcaaatcact aaaaattaga ggagaatgga
      841 atgaaacaag ccaggacggc taagaccgga agcacgaggc gtgcgtttat ctctaagagc
      901 ggactattgc tcgcgggtac ggtattggcc acttctaacg ccatgggagt tgaagcgagc
      961 agcgctgatt tttcggcggc cgcgcccatt ccccagcagg atgtcgccgc actacgcgcg
     1021 gaccttgtta ccgcgaacca tattttgtat gaccagggag ttgtggacgg ctatggccac
     1081 gtcagtgcgc ggcaccccgc aaacccaaat cattttctga tctcccactg gctggcgccg
     1141 gacatggtga cagatgcgga tattctggaa ctggatttcg attgcaaacc cgtgggtgga
     1201 gagaaagcgc gcttatacag cgaagtgttt atccacggtg aaatttatcg cgctcgcccc
     1261 aatgtgaaat cgattgtgca cacgcacgca ccggccgtaa ttctgatggg tatttccggc
     1321 gaaaccttga tgccgatgta ccagatgacc gggtacatgg gaacgggcgt cccgctcttt
     1381 gacattcgaa aagcagcggg gatgaccaat atgctgatct cagacgtcgc gcgcgcaaag
     1441 gcactcgttc aggcccttgc ggacaagccg gccgtattga tgcgcggcca tggcggcgta
     1501 gtgacaggga gcagtctggc gcaggccgtt gggcgaagcg tatacatgcg ggtaaacgca
     1561 gaactgcagg tgcaagtcct cgggaaaaaa atgacctacc ttgcgccgga ggaagcgcag
     1621 ctcgccagcg ataacgacta cccgaaggat tgggaaatgt ggaaacggaa agtcgcgggc
     1681 cagcgaacat agaaattcga ggggaattgt ccgggattta tcgaaacatc tagcaagcga
     1741 atcactgctt aaacaatagg ccggcgcgca atttatctct gggcgacggc cttctccatt
     1801 gctttccgca gatgcacata tcccactttt ccatagggct gcgcgccata ccagattcgt
     1861 ccctgcgcgt cttcaaacat gtcgcgcgtc ccgcgttctc cgtacggggt cggatactca
     1921 atcactttgc cggttttcgg atcgagtttg ccgatcacgt ccgtatacat cgacgcgtac
     1981 catagctgat cgttgtgatc cacgccgatg ccgtaaggcg tgggcatggg cccgggcaat
     2041 ttgaattccg taaactgctg cgtcttagga tcgaaacgcg tgaggtttcc ctcgaaataa
     2101 tccccgaacc aaatcattcc ctgcgaatcg atttttaagc ggcgcggtcg cgtgccggaa
     2161 tttggcggcg tccatttcgt taccttgttt gttttgacgt ctacttttcc aatgctgtga
     2221 tggtctttgg aattgaattc cgcgaaccag acatttcctt ccttgtcgac agcgatgcca
     2281 tacgtgtccg gcacttccgg aaaaaatgta tattttttgg tttccacgtc gaatttgctg
     2341 agcgggcgac cggaactcca aatgttgcct tgtaaatcag cgacggcggt atgccggcgc
     2401 gggtcgctgg tctgatctcc ctgtgtgtaa cgcaaattgg gccatggcgc attcggctcc
     2461 atctcgacgg gcggaggtcc gccatatggc tggcgaaaca cttcaaactt ttccgtgcgc
     2521 gtatcgaacc gggcgagggc attttgcttt tcgatggtga gccagacgta accatccgac
     2581 gtgggcaaaa tttcgtgaat gaaagggcgc gtcgcgtccg gcaatcgcca cgtgtgaagc
     2641 tcacccgtct ccggattcaa tttcgaaagg ccgccgccca tttccatcca catgtttccg
     2701 tctttgtcct gcttgctgct tcccggacgg tctttgggtt ctccggtcaa ttgatagtcg
     2761 acgtacacaa tggtcagcgc atcatcggaa aaatagtcgt gctcctgttt tactttctga
     2821 tactgcggca attgagcagg ggaagacggc gtttcggcgt cgggcccaaa caccgatccc
     2881 agatattcgg acacctgagc cgcgatgttg ggagaaattt ccgcgactcc gacctggcgc
     2941 atgtgctcga tcgcctccag ccatccttca cggtcgcgtc ccatcatgcc gatgcggctc
     3001 atggcgtggc aattaaagca ttcctgaagc accacatctt ttcccttgcc gtctggcagt
     3061 agaatgcccg cctgatattt cgtcagctgg gaccactgta ccgcgccttt cttcatcgtg
     3121 aagtttgcgt tagtcgtttt tccatcctcc accgcgatgt ctgttaaccg ggagggttcg
     3181 cttcggaatc cagtcgacgt cgcccatact tcatacgttc cggcgggcag gctgtcggta
     3241 cgaaattgcc cctgattgtt gctcagtacc atcatgatca ttttcgtttt tacattttgt
     3301 gcgcgtacga atgccgcccg aaagggagcg ccgtcgtctg cttttacggt gcccgaaatg
     3361 tcgccgccgg gagaagcgta actggaaact gcaaacgtaa acaggaaggc taagaatgcg
     3421 gaattaagaa tttttttcat tgcagtctcc accatttgga tcgggcagcc gcgccatatc
     3481 ttggcgagag tatatcgcag ttcgggttac actgacccgg aatcccgact ggaacatcgc
     3541 agttgtaaag gaatcttcta agttgaggaa ccacttcagg gagcggtaac gtaaaccgga
     3601 tttgtgaaga tccacagcgt ccatcgccgc catccgctga agcccggctg gcgcaggaaa
     3661 acttctgccc gataacgccc gggtccggac acgggttcgt cgatcgaccg ggtctcctgt
     3721 tcgtcaattt catggccgtc gcgaaacacc ttgatcaaag gcagacttga accggcggga
     3781 accgaaattt tcagcctctc attttctgaa gaatgcagaa tgtctccggg gcctccggag
     3841 acattcgcgc ccacaatacg caacgaaaag ccgtcggacg gatataggga gtctagtccg
     3901 caataggaat ggccatgtct gagagcgtca atcagctccg aagcactggc aagagcggga
     3961 ttgccgccac ccgaatccgg cgacatcagc aaatgttcgc gggctacttc cagtgatggc
     4021 gcgtactggg ggaatttcca gtcttttcca aatgccaatc gcacacgcgc gtgcgtatcc
     4081 gcggcgcaga tcccggcaag aggccgctgc tgcaaaagct catcccattt cgcaaagttt
     4141 tgttccggag tgcgcgccag gcgaacaaga gccagttgat tgttctcacc gtacatgatc
     4201 agcgcgttga tcaagtcaaa aaaattattc tgccgccaag tcgcgtcctc attccagatt
     4261 tccatgccgt cgaaatcggt cgtttgccag tcggtccagg gcgtgtgcgg gtgaaaagga
     4321 tgggcgagaa cgcgaaacgc tcctactgcg cccgccgcgg caagcaattc gcggtcatcc
     4381 ttcgtcttcg gttccttcca cgatgcggga atccccagcg ccagaaaatg accggccgta
     4441 gtgccgagct cctcacccga aataatcggc aggtccggtg gatcttcgtc cagatcgctc
     4501 tgggtcatgg cgatgttgtg atccgtgatg gccacaaagg ataaatgcgc ttgttccgcg
     4561 gcagtcatca cgtccgccac agtaccgctg ccgtcggatg cgcgcgtgtg aatgtgcacc
     4621 acgccgaccc gctccgagga gattttatct gtagcactgc gcggaagctt gaccagtagc
     4681 ccgtcgagga gaattcccgt gatcacaaaa gacgcggcca tgaagagaat aaaccataaa
     4741 ttaaacgtac ttcttggtac agatgtggag atgagcgcct cctttgccgg ctgaggaacg
     4801 ggcggctgaa aaatgcacct cgagtgggag tgacaactaa aatgccagct agagtgtgag
     4861 caacgacagc gaggctgtca agaagcaatc tatgttgttt tgaaagagat acataagcat
     4921 gagtgattta acgcaccaga gaattatggc attcgcagtg cgcaccgcgc tcgtgaatga
     4981 atttaattct atgttagagt aaaacgcgaa tcgctaaagg agaaccgatt gatttccttc
     5041 tttcggatga gaaagcgcgc gggattgctg tccttgcttc tcactctatt cttcgctgtg
     5101 ttcgtcgcgg ctgcgcacgc acaatccccc gaccctgcgg aaaccgcgga tcgccttccg
     5161 gtgcgtggca cggaaatcga aatctggacc ggtggcggcg cggacccgat tggcgcaaac
     5221 ggcgtcacgc agggcaatag tcagtggacc gctggcctgc ggtacggctg gattttgact
     5281 gacgcacatg gccccaagtt tctccgcggc aggtttgaat acgccgtcga cgcccttccg
     5341 gtggtgatgg tatttcagcc gggcggactc gcctatgggt ttggatttga tccgtggatc
     5401 atgaaatgga acctggagcc gcaccaccga atttcgccct acatcgaact gggcggaggc
     5461 ggggtaatca gcacgcgcga aattcccagg ggcgaatcca gattcaactt cacgccaacc
     5521 ggcgcgctgg gcgtcaatat tcttcgaggc aaataccatt ggagcgtcga cttccgttat
     5581 ctccatttct ctgacgcgga gatcacctcc tttaaccccg gcaccgacac atttggagtg
     5641 cgagtcgcct ggggtgttta cactcccagc cattagcgtt cgttccggag cgcagtcatc
     5701 tttcattcgt gaatcaatat ttcttgggta tgaaacataa atccgacttc atgcgtcatg
     5761 atgggaagaa gaaacggaaa agttccgtga tattcggcgt atcttatttc ttgacagtag
     5821 ctgcggcggg cagtacacaa gaaccattca ggaattgaat tttctcgcgt tgagccgagt
     5881 gacgggagtt cacactcaca ttgaatatca ggatggcagt gattgtggtt tcgctatttg
     5941 cgtgcctcgg cgcgacttgc gttggtgaga tgcgagcgca gaataaagcg tcggcaaccg
     6001 cgccgccggc taagttggcg gaagacgcga ggcgcggaga gggcttgttt ctgcagcgct
     6061 gctccatttg ccatctgccc agaaaactta aatttggatc gccgccagtc atcgggccca
     6121 gcctgagcgg gcaatttaaa gacgtcggtc cggagcagat gaaagttttg cgcggattta
     6181 ttttgaaggg cggcccggac atgccgggct tccaatatgg tttggaaccg aatgaagtgg
     6241 atgatctgat tgcttatttg aaaacgctat agctggtttt ttttgatcca gggaggctat
     6301 tcgtgccgcg aaaaatggct ttcgcagttg tgttcgccgc tgtggtctca cttggattgc
     6361 ttgtgggcgc gcaacgcgct ttttctcagt ttgctacctc ggtagaaacc gctgttccta
     6421 atgcgtcttt gctgggattc gtacattcat cggacggtaa aaatctggaa ggaattggag
     6481 tctcagcgcg caaaaatggc gagactttca caactaccgt atacaccgat gaatccgggg
     6541 ctttttggtt tcccgcgatg gcgaccggtc agtacaaact atgggcgcag gctgtcggct
     6601 tcgaaactta taaaacggat gtgacgtttt cggatgtacc ggtaaggaat gtgagcatca
     6661 ggctggcgcc gctcgccgac ttccacaagc aactttccgg caccgagtgg gccgagagtc
     6721 taccggaaga tacgcccgat gaccgccgga tgaaaaccgt tttcatcaac aactgcagcg
     6781 gctgccatca agtcagcttc ctgttgcaga atcgttttga cgcggcgggc tgggcagccg
     6841 tgattacttt aatggaaaaa atgcagtcca tcggttacgc gcctgcagat atcgctccca
     6901 atgttgtgat tcattcgtac aagccggaac tggcctcgtt tttggggcgc gttcgaggcc
     6961 cggcgaatgc ttcactgaat tggaagctgc tgcctcgccc cacgggagac gccgccaaaa
     7021 tcgtaatcac tgaatacgat ttagcgcggc cagatatgcc cggctggacg atggagcaca
     7081 acgggacgga ctggtcggag ggaacgcctt cacgttggga tgggcgagcc gctcacgatg
     7141 tggcggtcga cagaggtggc ttcgtatggt ttgcggatga cgcgacgccc ggccggactt
     7201 tggccaagct ggacccgtcc accgggaaaa ttacggacta catgctggcc gatcagaaca
     7261 acgcggcgga aagttcgcac gcgctcgtct ttgataaggc gggcaacatc tggtttgcca
     7321 acggtacaga aggaagtcct actaaattcg atcccgagac cggcaaattt ttcaaatatc
     7381 cgcgtcccaa agattttcca ttttccggcg atttcatcac catggataca aaagggaacg
     7441 tgtggtcacc gcaccgcgaa ggcgcgtaca aactcgaacc cctgaccggg aagtatacaa
     7501 atttcgcgat gggtccgggc aaagcgaatt acgacatcgc cgcagacaac gaagaaaaag
     7561 tgtggatttc gcagcctggc ggcaatgaca tggaaatgat cgattcacgg agcggaaaaa
     7621 tcgagcggct ggatttgagc ccggtcattt cgcgggatta tgaagtgact gcgaaggatc
     7681 gcgaacttgc cgcgggcctc gaactgacgc cgaacaccgc gaccccgctc gacaagggac
     7741 cccgccgatc tgccgccgac cgcgacggcg atttcatttg ggtgtgcgaa ttttttgcgg
     7801 accgcctggc caaaatcgac gcgcgcacaa aaaaaatcac ggagtacccg ctgccgcatc
     7861 gcttcagcca accgtactcg gcaaccgtcg ccaaggatca caccgtgtgg atcacgatgc
     7921 tcaactccga ccgcattgcg aagttcgatc ccgcgacaga aaaattcacg gaatatgttc
     7981 tccctacgcg tggcactgaa atccgccaca ttcaggtgga cggcagcaca accccgccga
     8041 cagtctggct accatacgac cgcaccaaca aaatcgccag aatccaattc cgataggatg
     8101 aagtagcata ggcttcagtc tgtggaattt agcccttgca aggactaaat tccacaggct
     8161 aaagcctatg ccacacctag ttcctaaaat caaaaattat cacggaatta aaatggtttt
     8221 gcccagcgcc ccagtttcga gaatttcttt atgagcgcgt gcggcctctc ccagcggcaa
     8281 ttccttcccg accaccggcc gaatcgttcc attctccagt cccgcaaaca gcccggcatg
     8341 tacgtcggct tcgtcagcct cagaaatgct ccacagcaca aatccgcgta cggaagatgc
     8401 ccgtgccatc agatcgcgcg gcgtaatcgt cacctcgccg cggctgccga taacgatcac
     8461 gcgtccaaat cgtgccagca ttttcaaatc agccgagaga ttcacgttcg cgagcatctc
     8521 aatcacgacg tctaccccgc gtccgttcgt ggccgtcatg atttcgtccg tgtatcttgc
     8581 tgcgcgatga ttgaacgcat actgcgcgcc ttcacgccgt acaagttcca atcccttttc
     8641 ggttcccgcg gtcccgatca ctgtcaggcc catcgcacgg gccatctgca ccgccgcaat
     8701 tcccacgccg ccgctcgccc catgcaccag gagcgtttcc gaagcgtgtg tgtgcgcgtg
     8761 atgatggagt gcatgatacg ccgtgccgca ggccacataa attcccgcgc cttgattgaa
     8821 ggagatgtta tcgggcagcc catgcagctg cgattccagc gcgagcgaat attccgcata
     8881 cgtgccggtc accggatggc ccacatacac tcgatcgccg accttaaact ttgtgacgcc
     8941 ggcacccact tcgtgcacca cgcccgcgcc ttcgccgccc ggaatatagg gcaggggcgg
     9001 cttgatggcg tacgtgccgt tgcgcatgta cgtgtcgaac gggttcacgc tgccggcatg
     9061 aatgcgtacc accgcttgtc caggcccagc cttcggtgta gggaattctt ccagcttcaa
     9121 tacttccggc ccgccgaact cgtgcacgac aattgctttc atttttttct cccgagcaga
     9181 aactgtgttt ctagtatcga attctggcga ctcaatctct caatgtcaat gcgtcacccg
     9241 cagcgcaggc ttcagacttt ggcactcaga ttttgcggta cgaaattcaa aagccacagg
     9301 cggaagcctg tgctactcaa cccaaacgca atttgtgcta cattacgcgc tctaacgaag
     9361 tattcaatat ttctgcggag aaccaatgat tatcgactgc cacggccatt acacgtccga
     9421 accagctgcg tttatcgaat ttcgcaagca gcagattgac gcgttcaata agtcgtccgc
     9481 cgctcccgcc ctgccgcaaa tcacggacga tcaaatccgc gaaaagctcg aacccgcgca
     9541 attaaagttt caacgtgagc gcggcacaga cctgacaatt ttttctccgc gcgcctccgg
     9601 catgggccac cacataggcg atgcgaaatt aagtgcgtcc tggacgcaaa tctgcaacga
     9661 catgattcac cgtattgtga atctctaccc aaagaatttc attggcgtct gccagttgcc
     9721 gcaatccacc ggcgtgcggc cagagacgtg catccccgaa ctggagcgct gcgtaaaaga
     9781 attgggattt gtcggctgca atctgaatcc cgatccgtcg ggcggttcgt ggaaggaacc
     9841 gccgctcacc gacaagtggt ggtatccgct atacgaaaaa atggtggaac tcgaagtgcc
     9901 cgccatggtg cacgtcacag ccacgtgcaa ccctgcattt cacaccaccg gttcccatta
     9961 cattaacggc gatacgactg ccttcatgca attccttacg tccgatttat ttaaagtttt
    10021 tccgaccctg aaatttataa tacctcacgg cggcggcgcc gtcccttatc actggggccg
    10081 ctaccgcggt atcgcgcaag acatgaagct tccgccgctt acggaactgt tgctccgcaa
    10141 cgtgtacttc gacgcgtgcg tctatcatca acccggaatc gactgcatga ccaaagtgat
    10201 tccctcggac aatattttgt tcgcgtccga aatggtcggc gccgtgaaag gcatcgatcc
    10261 agaaaccgga aattatttcg acgacacgcg gcgttacatc gacaactcca ccatactaaa
    10321 cgcttccgac aagaagaaaa tatacgagtc taacgcattg aaagtatatc cgcgtctcgc
    10381 aaaacaaatg cagaagcagg gaattgccgc gctgctgtag taagcctgaa ggcgttcccc
    10441 tccgcgccaa ggcacgccaa ctaaccttga caggttccct gaaagttaac cggatgcttg
    10501 aaaacacccg agcccacgga cactttgtct tgacgaaggg aaatgcattt cgcacaatca
    10561 actcatcgaa ttagctcctg tgcggctcgc gcaatgttca cgcctagagc gcaggcaaga
    10621 cagacacgca tcttcggatc aaaagaggta gcatccatgg gaaaaattgt agcggcagcc
    10681 ggagtcgtcc acgcgccaca actgtttgta agacccccca gcgaagaccc cgcgcaactt
    10741 gacgctgaca tcgccgccat gcgcctcgtc ggaaaagatc tcgacgaaac aaagcccgac
    10801 gtcgcgatcg tcatcggcaa cgaccacatg gagacatttt ttctttcctc ggttccaacc
    10861 ttcgccatta tgtccggcga acgcgcgcgc gctgaattcg cgcgaaaatc ttacgatctc
    10921 ccggtccatc aaggcctcgc cgaagatttg ctggacaagt tggtgaatga cgattttgat
    10981 atggcctatt cgcaggacgc cgtgctcggc cacgcctttg ccgcgatgta cgagtgggtg
    11041 atcgaaggcc gaaatatccc cgtcgtcccg atctttgtga acgcctacct gccgccactg
    11101 ccgactgcaa ggcgttgcgc gcaacttgga aaagcgattg ccaaggtcat cgccgcgcgt
    11161 ccggaaaaag ttgccgtaat cgccagcgga gggatgtctc atttccccgg aacatggaaa
    11221 tatccagcgc ccgatttcga tttcgattat tgggccatcg cgcaaatgga aaaaggaaat
    11281 cacgaagctt tactaaatct cacatcgcag cagcttgacg ttgtgggaaa cacggaaatg
    11341 ctttcgtgga tgatcatgtt cggaatgatc ggcgatcagc ccggcgaact cattacctat
    11401 cagcctacat ggcaccacgg ccacgctgtg atgcgcttcc tgcccgacaa gcgaaccgga
    11461 aaactttccg acgaaggaaa gaaaggcccg gccgagtacc atttcaaagg ttccgagggc
    11521 tacgaattct acaagcaccc ttcaccatcc gcctacaaac tgaataaaat tttgtatgac
    11581 ttgcgcttca agcccagcct gcgcgttcgc ctgattaacg acattccggg cgtcgcctcc
    11641 gaatacggtt tgccgctcga cgaagctaaa gttctggaaa cgttgcagga tgaggacatc
    11701 gaaaaattcc gcaacggaaa agttcacccg ctggtgcaag ccggcgcgca cccgctcgga
    11761 atgtggatga gcgtaatcgt atttcacgcg gagttgcgca gattgcgcgc cgcagccgca
    11821 aaaagtgaca cagaaacagc cagtgccacg caatagtttt tagtagcacg cgcgtcagcc
    11881 tgtggctttt ggttttggga gagctagaat taaaatccac aggctggagc ctgtgctact
    11941 cactccaaac tcaaaatttc agttcacagt ttcacccggt ttcggaggat tcatgcccaa
    12001 gatgcgcgcg ttgcaaatcc ccaaagccaa ggaaccgttc cagctggttg agcgggaaat
    12061 ccctgccccg gcaaccggcg cagtgcgcat caaagtccag gcgtgcggca tttgtcacag
    12121 cgatgaatac gcgaaagaag gctcgcgccc cggcatcacc tacccgcgtg ttcccggaca
    12181 cgaaattgcc ggtgtgattg atgcgattgg agacggtgtt caaggctggg aagtcggaca
    12241 gcgcgtcggc gtcggctggc acggcggaca ttgcggctac tgcaattcct gccgccgcgg
    12301 cgatttcgtc acgtgcagca aaggccgcca gatcaccggc ctcacttgcg atggcggcta
    12361 caccgaatac atgctcatct cgagcgccgc actcgccgca attccggacc aaatttccgc
    12421 cacggacgct ggcccgttgc tgtgcgccgg cgttaccact ttcaattcgc tgcgaaacag
    12481 cggcgcgcgc ccgggagacc tcgtggcaat cctcggtctc ggcggcctag ggcatctcgg
    12541 cgtgcaattc gctcacaaga tgggttttga aaccgtagcc attgcacgcg gaaaagataa
    12601 agaagcattc gcgaaaaaac tcggcgctca acattatgtc gacagcgaat ctcaaaatgt
    12661 gccggcggaa ttggaacagc tgggcggcgc aagagtgatc ctctccaccg taaccagcgg
    12721 caaagccgtg aacgcagcct taaatggaat gggaattaac gggaaaacaa ttctcgtggg
    12781 caatcccgat cagcccctgg aaatcacagg ccgccttctc atcgcaggac gccgctcgat
    12841 cagcggttgg ccctccggct ctcccatcga ttcgcaggac actttaaaat tctgcgcgct
    12901 cactggcatt cgccccatga cagaaattta tccgctcgaa cgcgcccccg aagcctacga
    12961 ccgcatgctg agcggcaaag cccgcttccg agtcgtcctg agcactggcc tgtaagtagc
    13021 agaggctccg gcctgtggaa tttagacgaa gcaagaatta aacctctaga agtttaattc
    13081 tgtgcaggac ccagtcccca caggctaaag cctatgccac aacttcaccg aatggaccgc
    13141 aactctgaac agccctatac tctccttgtg aatctcgacc gcgacctcct cactcccctg
    13201 cgccgctact ggggctacga cgccttccgc cctctccagg aacgcatcgt tcgcagtctc
    13261 gtcgacggcc gtgacacctg cgtcatcatg cccactggcg gcggaaaatc tttgtgctat
    13321 cagcttcccg cagcgatgtc cgagaacaga accgtcatcg tcatatctcc cttaatcgcg
    13381 ctgatggaag atcaagtcgc gcagttggga caaatgggaa ttccggcagc gttgctgaac
    13441 agcacgatca agcctgcaga ccaatcgcga atcatcgaaa aagcgcgcgc gggcggctat
    13501 cgacttttat acctttcgcc agaaaggctc gcgcgcgaag atacgatcga atggctgcgc
    13561 ggcgtgccga ttatgttttt cgcgattgac gaagcccatt gcatttcgga atggggccat
    13621 gaattccgcc cagattatcg cctgctcagc cgtctgcgcg cgcattttcc ggatcgcgcc
    13681 atcgccgctt ttacggcgag cgccacgcag cgcgttcgcc acgacattat tcgccaactc
    13741 gaactgcgca atcccgacaa atacatcgcc agctttcatc gtccgaattt acgttacgtc
    13801 gtgcgcgcct gcgacgcaca ttcccaaaca gaacttctgt tgcgcgcgat gcgcagctac
    13861 ggagacgaga atctgattgt ctacgcgccc acaattgcca aagtggaatc cactgtggat
    13921 tttcttcagg aaaatggaat tcccgccgtc gcctaccacg gccagatgga cgccgccgac
    13981 cgccgtcgca accaggaacg ctggatgacg gacgaagtgc gcgtgatggt cggcaccatc
    14041 gcgtttggtt tgggaatcaa caaagccgcc gtacgagcgg tgattcacct ttcgcttccc
    14101 aaatccatcg agcagtatta ccaagaagct ggccgcggcg gccgtgatgg ccaacccgcg
    14161 gattgcgtgc tcctttggca aaaaagggat gtcggcctgc atgcctattt tatcggccag
    14221 atgaatgatc ctcaggaaaa gcagcgcgcc tggcaacgct acgacgagat taaaggcttc
    14281 gttgaatccg cgcattgccg acaccaacga atctgcgagc attttggcga aaataaaaaa
    14341 tgggttgcgt gcggcgcctg cgatatttgc agctaccagc cggaatggtt aatcgctccg
    14401 gtacaggagg ctccgcgcaa atcgaaacgt ccaaggtcgc gttccaccgc gctcacatcc
    14461 gcgcccattg cgcgcacatg ggaaaagccc gcgcccaggc cggctgccga gcgcgcaccg
    14521 aacgccgtgg aagttgatcc gaatctgcgg gagtacatgc gtgagtggcg aaaggacatt
    14581 gccaaagagc agaatgttcc cgcctacgtc gtcatgcacg acgtcacgct cgatgcaatc
    14641 tgtgccgtgc gccccacgag cattgcgcgt ctcctcttaa ttaccggaat tggcgagcgc
    14701 aaagccgagt tatacggaag ccagatcctc aacgcgctga agcaattcga aggcggagcg
    14761 cgcgccactg ccgcggaaaa gaaaatcact ccgagcgaag agacaattca gctgatcgag
    14821 ggagggaagt cgctggagga aattgccgtg attcgcggac gaaggatttc caccattgtc
    14881 agcatggttt cagacctcgt cgagcgcggt ctcgtggaat ttcacacgga atgggttccg
    14941 gaaaatcgcc gcaatcaaat tgaatccgtc gccgccgatc tcggcttaga cagagtcacg
    15001 cccatcaaga acgaactccc cgaagatttt acctacgatg agattcgcct ggtagtttca
    15061 aagctccgcc gcgacagcga cagcaaaccc ttgggggcaa caacatagca tcttccagcg
    15121 cggaaaaatt gttgggcaat gttcggatag ggcagccttg cttgtgcgag ttttgcaagg
    15181 ttcacatagc caagagtggc tgtgctacgc gaatcggctt tagcttccga ccggtactag
    15241 ctaaaagtgc atccgctgta cctatgaagc gagttccaac gaagccacag gatcattgcc
    15301 cgttcccagc gaaaccattt ccagctcccc catcatgcga tggataatta cccaagcaac
    15361 aatgtaaaag caactcatcc ccatcagcac aggcacgtag ccgacatgcg gaatcaccgt
    15421 ccccggaata attcctgaaa acagcgcgcc gcccaatcct ccaaggcaaa ctccaaatcc
    15481 gttcgcgctg cccaccgccc gcgaagggaa aacgtccgca ggcatgatga ataaatttgt
    15541 catccaggct tgatgcgcgg ctgtcgccag gccaaagagc aacaccgcca accttgcatc
    15601 ctgcaccacc acgcccagcg tgcacgcagg catcaccacc gcacaaaaca gcagcgtcat
    15661 tttccgcgct ttgcccaccg tccacccgcg cttcatcaga aagctcgaaa caacgccgcc
    15721 catcaacgcg cctaacgcgg atatgctgta cacgatcatc agcgccatgc cccgttgttg
    15781 cggcgtgaag tggcgcatgt ctgttaggta cgtcggcaac cagaaaaggt aaaaccacca
    15841 cacaggatcg gtcaaaaatt t
//
LOCUS       DASZMB010000158         2701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_730858, whole genome shotgun sequence.
ACCESSION   DASZMB010000158 DASZMB010000000
VERSION     DASZMB010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_730858"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..1725
                     /locus_tag="VG272_07255"
     CDS             34..1725
                     /locus_tag="VG272_07255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF13516.1"
                     /translation="MAEKSSVNKIKSAAKLIKKLRTRVHELEETFLAIKRGRVDAVVV
                     NGVEGDQVFTLQGAEHPYRVLVETMNEGAATLDEDGTVLYANASFGALLGVPTDAVIG
                     NSLQTYLEPTEQIKLNTLIGESLKDESHGEVILATSDGKRRLVRMSLSPIHEFGVRTI
                     CVIATDMTELSGANEALKASEDILRNLSGRLLRLQDEERRRISRDLHDVTGQKLAVLS
                     MNLSSALKNKAATKNEEMNRLLTESIGLSNDVNKEIRTLSYLLHPPLLDELGLSSAIE
                     WYAQGFENRTGIRTTVDIPTGFMRLAPDAEVALFRVVQESLVNVHRYSGSDTAFVRAN
                     GDEAEVRLQIGDAGIGIGKQLNTASLTSTVLGVGIQGMKERLRQLSGRLEITSSPGRG
                     TLVTAILPVCTRQAKISTDYVESETGNAHDAVMEVVAEPNGWRKRILIADDHDVLRRG
                     IRTMIESDPGLEVCGEAVDGKDALEKTLSQAPDLVILDINMPVMNGLDVLRQIVRHRP
                     KIKVLAFSVHDSKQIVEEILAAGAHGYLSKATAGQHLVHEVRALLNESVRSVTAN"
     gene            1933..2322
                     /locus_tag="VG272_07260"
     CDS             1933..2322
                     /locus_tag="VG272_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF13517.1"
                     /translation="MPRSILIVDDSQAVRHLVRSYLESQLDQVACTEAVNGRDAIERV
                     RNAKPDVIVLDVSMPIMNGLAAAPFLHDMAPLAPIILFTLHKDVVSEKQAVAVGIRAV
                     VSKMDEVEVLLKSILKLTAIVRSTMPS"
     gene            complement(2415..>2701)
                     /locus_tag="VG272_07265"
     CDS             complement(2415..>2701)
                     /locus_tag="VG272_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HWF13518.1"
                     /translation="STYSLRVQGNSMNPLIYDGYILVVDSSQTNRAELNNKIVIAWNK
                     DFGLTVSRFRRYDHTEVLQPENREYDSVTIDRTNKWKIIGRVLWWIGRAP"
BASE COUNT          633 a          712 c          744 g          612 t
ORIGIN      
        1 agttctcctt ggactggacc tgaaggaata ggaatggctg agaaatcttc cgtcaacaaa
       61 attaaatccg ccgcaaagct gataaagaaa ttgcggacac gtgtgcacga actcgaggaa
      121 actttccttg cgatcaagcg cggccgggtg gacgcggttg tcgtaaacgg tgttgagggc
      181 gaccaggttt ttactctgca gggcgccgag catccctacc gcgtgctcgt cgagaccatg
      241 aacgaaggag cggcgacgct cgacgaagat ggcacagtgc tttacgccaa tgccagtttc
      301 ggcgcgcttc tcggggttcc taccgacgcc gtcatcggga attcgctgca gacgtatctc
      361 gagccgacgg aacaaatcaa actcaatacc ctgattggcg agtcgctgaa ggacgaatcg
      421 cacggcgagg tgatcctcgc aacgtccgac ggcaagcggc gtctggttcg catgtcccta
      481 agcccgatcc atgaatttgg cgttcgcacg atctgcgtga tcgccacgga catgaccgaa
      541 ctgtccggcg cgaacgaagc cttaaaagcc agcgaagata ttttgagaaa tctttcgggc
      601 cggctgctgc ggcttcagga tgaggaacgc cgcaggattt cccgcgatct gcacgacgta
      661 accgggcaaa aattggcggt tctctcgatg aatctttcga gcgcgctaaa gaacaaagct
      721 gcgacgaaaa atgaagaaat gaaccggctg ctgacggaat ccatcggact ttccaacgat
      781 gtgaataagg aaatccgtac gctgtcctac cttctgcatc cgccgctgct cgacgaactc
      841 gggctctcgt cggcgattga atggtacgcg cagggctttg aaaatcgcac ggggatccgc
      901 accaccgtgg atattcccac cgggttcatg cggctggcgc cggatgccga agttgcctta
      961 ttccgtgtag tccaggaaag tctggtcaac gtgcatcgct attccggaag cgacacggct
     1021 ttcgtacgtg cgaacggcga cgaggcagaa gtgcggctgc aaattggcga cgcgggaatt
     1081 ggcattggca aacaactgaa tacggcttca ctgacctcca ctgttctggg agtcggaatt
     1141 caaggcatga aggagcgcct tcgccagctt tccggacggc tggaaattac gtccagtcct
     1201 ggccggggaa cgttggtcac cgcgatactt ccggtgtgca ctcgccaggc aaaaatttcg
     1261 acggattatg tggaatccga aaccggaaac gcccacgacg ccgtcatgga agtcgtcgcc
     1321 gagccgaacg gctggcgcaa gcgcatcctg atcgccgacg accatgatgt gcttcgcagg
     1381 ggaatcagga cgatgatcga aagcgatccg ggcctcgaag tgtgcggcga ggcagtggac
     1441 ggaaaagacg cgctcgaaaa aacgctctct caggccccgg atctcgtgat tctcgatatc
     1501 aatatgcccg tcatgaacgg ccttgatgtg ttgcggcaaa ttgttcggca ccggcccaaa
     1561 ataaaagtcc tcgcattttc tgtacatgac tcaaaacaga tcgtggagga aattcttgcg
     1621 gccggcgctc atggttacct ttccaaggcg acggcagggc agcatctcgt ccatgaagtc
     1681 cgcgctttac tgaacgaatc ggtgcgttcc gtcaccgcaa actagggaac ggcgatagct
     1741 gtctcacgct gctggagaag cactttgccc gccatccgtg cctcgcaggc ccgctcccga
     1801 agttttattt tttggaaaat accgcatatt ttctactgtc taggggtatg tgggtattgt
     1861 tttcgtcttc taagagagta cgcttgcgta cataggaata agtcagcatt ggctgcatcc
     1921 cacggagcgc caatgccaag atcaatttta attgtggatg atagtcaagc cgttcggcat
     1981 ttagttcgtt cgtatctgga atcgcaactg gaccaggtgg cgtgtacgga agcggtgaat
     2041 ggacgggatg ccattgagcg tgtccgcaac gccaaaccgg acgtaatcgt tctagacgtc
     2101 agtatgccca ttatgaacgg tttggctgcg gctcccttct tgcacgatat ggcgccgctc
     2161 gcccccatca ttctattcac gctgcacaag gatgtcgtct cggaaaaaca ggcagtagcg
     2221 gtgggaattc gtgcagtggt ttcaaaaatg gatgaggtgg aagtattgct gaaatctatt
     2281 ttaaagctca cggcgatcgt tcgctcgacg atgccctcgt aacttgcctc gagctttgtt
     2341 tccgttgccg ggtccagctg ggcattaaat cttcgcgcac gaattcgagc ttcagaaatt
     2401 ccgcgcgccg atcactacgg cgctctccca atccaccaca aaactctgcc gataatcttc
     2461 catttatttg tgcggtcgat cgtgaccgag tcatattccc gattttcggg ctgaagcact
     2521 tccgtatggt catatcggcg gaatcgtgaa accgttaatc cgaaatcttt gttccaggca
     2581 atcacgatct tattattaag ctcggcacga tttgtttgcg atgaatccac tactaaaata
     2641 tagccgtcgt agatcaaggg attcatcgaa ttgccttgca cacgcaagct ataggtggat
     2701 g
//
LOCUS       DASZMB010000159         2394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2909353, whole genome shotgun sequence.
ACCESSION   DASZMB010000159 DASZMB010000000
VERSION     DASZMB010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2909353"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..1517)
                     /locus_tag="VG272_07270"
     CDS             complement(216..1517)
                     /locus_tag="VG272_07270"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWF13519.1"
                     /translation="MTRILLTTVDQDFGLYDYYRENAPERFRWRFQMPRRISFGLRFL
                     RQNLPEINIVEYPTRTEYLAALKKGWDVVGFSFYLEESNHVEGMAEEARKAGVKSLWA
                     GNYGALTPSLQSRFDNVFSGYSEDAVAQALGKTIDVVRHPPLVSEFRLPGGWPMPIGI
                     LFTTRGCSFPCTFCQTTAFAPRPKAIPLESIEEALRFYVAHGIHYVLILDENFGNIPQ
                     QAEAVIEMLARYKVNWLVQSRVDLFLRNFDMWIAKGMEGALFGIESFHQEVLKQIHKG
                     EKVEAVMELAQRLNRAGKYAHGYYIIGFPSETPESIAADLKTLASMELDVTQITIVTP
                     HPRTQLWNELDSTYGIFEKNLSKYDTKHLVWNHPQCPPGSLESLLKQGFEVCYGKDWL
                     RRTSGKFLKSRLRRGDLGNLFLSPLHARWAEPRRLPYLEAR"
     gene            complement(1751..>2394)
                     /locus_tag="VG272_07275"
     CDS             complement(1751..>2394)
                     /locus_tag="VG272_07275"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HWF13520.1"
                     /translation="EGVQVESQNYEHAGKSYVLVNALGSQEVPDGKYLYDPASKQIEI
                     QWIQGIGSDAAPAPQARLMATVINGILTQRLPWRLVLLGVFLVVTIELLGVRSLAFAV
                     GSYLSIATTMAIFAGGAVRWLAERNKPRTAEAESEVSPGALYSSGLIAAGGVVGLLAI
                     IIKLMEIRGWATPDKFNVGTRWIGGLATNGWLGVVMFLLLASSLFVFARKKLE"
BASE COUNT          559 a          690 c          621 g          524 t
ORIGIN      
        1 aaaatcttcg aaatccatgc gcgcaaatct atcactattt cacatcttgt caaggaattt
       61 aaatccatga aaagtataat ttcctcaaac tgatgaagcg agctgattcc gatcatctgt
      121 atcagtcaaa ttactgatag agcagtagtt acgagacatt ctacatcata acagagggaa
      181 ctccggggag ggcgtcggaa ttttcacgag agggactaac gggcttccaa atacggaagc
      241 cgccgcggct cggcccagcg ggcgtgcaac ggactcaaaa aaaggttccc cagatcgccc
      301 ctgcgcaacc ggctcttcag gaattttccg cttgtccgcc gcagccaatc tttgccgtag
      361 caaacttcaa acccctgctt caacaaagat tccaagcttc ccggcgggca ttgcggatgg
      421 ttccacacca gatgcttggt gtcatatttc gataagtttt tttcgaagat gccgtatgta
      481 gagtcgagct cgttccacaa ttgcgtacgc gggtgcggcg tcacaatcgt aatttgcgtg
      541 acgtcgagtt ccatggacgc cagagtcttc agatccgctg cgatggactc cggcgtttcg
      601 gaagggaatc caatgatgta gtaaccgtgc gcgtattttc cggcgcgatt cagacgctgg
      661 gcgagttcca tgaccgcttc caccttttcg cccttgtgaa tctgcttgag gacctcctgg
      721 tgaaaactct cgataccaaa cagcgcgcct tccatgccct tcgcgatcca catatcgaaa
      781 ttgcgaagaa acaaatcgac gcgggattgc accagccagt tgactttata tctcgcgagc
      841 atttcgatta ccgcttccgc ctgctgcgga atgttgccga agttttcgtc cagaatcagc
      901 acgtagtgaa tgccgtgcgc cacgtaaaag cgcagagcct cttcgatgga ctcaagggga
      961 atggctttcg gcctgggcgc aaacgccgtc gtctggcaga acgtgcatgg gaaactgcag
     1021 ccccgtgttg tgaacaaaat tcctatgggc atcggccagc cgcccggaag ccggaattcc
     1081 gaaacgagag gtgggtgcct gaccacatca atcgtcttgc cgagcgcttg tgctaccgca
     1141 tcttcactgt atccgctgaa cacattgtca aatctggact gcaaggaggg agtcagcgcg
     1201 ccgtaatttc ccgcccatag ggatttcact cccgcctttc gggcttcctc agccatgccc
     1261 tcaacgtgat tgctttcctc aaggtaaaac gaaaagccca caacgtccca gccctttttt
     1321 aacgccgcaa ggtactcggt tcgcgtcgga tattcgacga tatttatttc aggaagattc
     1381 tgccgcagga accgcaagcc aaaggaaatg cgccgcggca tttgaaagcg ccagcggaag
     1441 cgttcgggcg cattctcacg gtaatagtcg tagagtccaa aatcctgatc gaccgtggtc
     1501 agtaaaattc gagtcatcgc aatacctcaa cgagggatta gggatgtgcg ggttggctgg
     1561 ccaggatacc atgcccggta agtctaacac gtgactacac agatcgtttc ttcaagccgt
     1621 ccggccgggt cgcgaacgcg ttttaagttt atgcgcgccg gaaatcgtgt ttgcgaagat
     1681 cggtaatgtt gcagtagcag gtgcacgtca cgtcacgcac gatgtcaaca gagccagatt
     1741 ggtctaaaac ctattccaat ttcttgcgcg cgaacacgaa cagcgaggaa gccagcagca
     1801 ggaacatcac gacgcccagc caaccattcg ttgccaggcc gccgatccag cgcgtgccga
     1861 cgttgaattt atcgggtgtg gcccacccgc ggatttccat cagcttgatg atgattgcga
     1921 gcaggcccac gacgccgccc gccgcgatta atccgctcga atatagagcg cccggactga
     1981 cttcgctctc tgcctcggcg gtgcgaggtt tgttgcgctc ggccagccag cgcaccgcgc
     2041 caccggcaaa aatcgccatc gtcgtcgcga tggaaagata ggagcctacc gcaaacgcca
     2101 gcgagcgcac gcccagcagc tcgatggtca ccaccagaaa gacgccgagc aacaccaatc
     2161 tccagggcag ccgctgcgtc aggatgccgt tgatcactgt ggccatcagg cgcgcctgcg
     2221 gggccggcgc ggcatcactg ccgataccct gtatccattg aatttcaatc tgcttcgacg
     2281 cgggatcgta aaggtatttt ccgtcaggca cttcctgcga accaagcgcg ttcaccagca
     2341 cgtaggattt gcccgcatgc tcataatttt gcgactcgac ctgcactcct tccg
//
LOCUS       DASZMB010000160         5236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3184400, whole genome shotgun sequence.
ACCESSION   DASZMB010000160 DASZMB010000000
VERSION     DASZMB010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3184400"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VG272_07280"
     CDS             complement(<1..356)
                     /locus_tag="VG272_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF13521.1"
                     /translation="MRNDTQKGFSLIELLIVVAIILIIAAIAIPNLLRSKMAANEASA
                     VASLRTYNTAIVSYSTTYGTDPATDLSALGPATTPSSTAADLVDNLLGTASPTKSGYT
                     FSYTAGTASNGIISQYS"
     gene            857..2176
                     /locus_tag="VG272_07285"
     CDS             857..2176
                     /locus_tag="VG272_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13522.1"
                     /translation="MMDFTKRLFSSALFITISAFIMRMAVLIYSWHATLIPARYFLPY
                     GYELGHVAKAIASGQGFSSPLRFVDTGPTVWFTPIYPYLVAGIFKIWGIYTNASKLII
                     MTLNCAFATLTVVPIYCSAQRTFGKAVAVGASWTWVFLPTALFFPITWIWDTAMAALF
                     LSLIFWATLVVREKTTVLPWAGYGALWATGVLINPSMLSLLPFLAGWALWEARRGEAQ
                     WPKFAAAMLFIFALGMVPWTVRNYRVFGKIIMLRSNFGLELWLGNNPNVPDLWSPWLH
                     PNDSRDEALKYKNMGEIAYMEEKQQEAFAFMRTHPADTMNFIYRRFIENWLAISDSPT
                     AFWGNTPFYIRAFFVLNVILSLFTFVGVLFAYRFRIPEAFPYAMVPLIFPIIFYITHA
                     SLRYRFPMDPIMMILATYGVASVVRLLRGKSAHLSDSVSPETSLPTA"
     gene            complement(2313..3704)
                     /locus_tag="VG272_07290"
     CDS             complement(2313..3704)
                     /locus_tag="VG272_07290"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWF13523.1"
                     /translation="MAHILVVDDERSICELLEITFRKEGHRVEVALNGEAARRKLESS
                     IFDIVISDIRMPDTTGVELLKFCREISPSSYFMLITGVPTMETAIAAINAGADRYVIK
                     DHDLVDQLRRAVRQVEETLKLKHEAGYLRRELRRLTGQDNIIGHSPKMRAIFDMITTV
                     APQTSRVLITGETGTGKELVARAIHENSARSKEPFITINCGAFPETLLESELFGYLKG
                     AFTGATENRQGLFRGAHGGTIFLDEIGNMSLPMQVKLFRVLQEGKVRPVGSNEEFEID
                     VRVIAATNKDLDKAIASGEFREDLFYRLSVIPIHLPPLRERREDIPLMARSFLERFRK
                     VMDKPIEGITPATMTLLEAYDWPGNVRELENTLERCVALETGTMISTAVLPEKIQRGV
                     THDNHNHASTSNGHFNIPEGGIDLEKSVQEMEKAYILSALQACDGIGTHAADLLKMTY
                     RSFRHYSKKYNIR"
     gene            complement(3733..>5236)
                     /locus_tag="VG272_07295"
     CDS             complement(3733..>5236)
                     /locus_tag="VG272_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF13524.1"
                     /translation="LQVSTRYFIPLMILWYTMAIVYSILTRWMPDAKWHAGVQLIFDL
                     LIVTGLIYLTGTQDSYFIWLYPLAIIVASILFSRKVTFTVAGFSFVLIGGLAELIFYN
                     IIPRMQIAVPSSRQLQTWIFSNLFYFMAVAYLSSLLAQTLRRHSEELEVKRGQLQDLQ
                     AFNQDIIHSMRGGLLTTDLEGRILLLNRTGEEITGCQFRNVKRSNVQKLWPSFWLPGD
                     VSEDGKLPQRREVDLMTPDGQQRFIGISVSPLRTGERRTSGYVFNFQDLTDLKRLEQE
                     VATKERMAALGRLSAAIAHEIRQPLTAMAGAVKELGRLVPLQEDEQHLVGIVSRESER
                     LNKIITEFLNYSREKTYEFSEEDVSTILDETLTLLERNPQVDGKYKIERAFTEKEVRV
                     RVDRNRIKQVFWNLSDNAMRAMPDGGILTVSLEAAPFWVRIRFRDTGMGLDPRQRQKI
                     FEPLQSNFVGGTGLGLAIVYQIVQAHSGRVSVFSEKDKGAEFTIDLPRVA"
BASE COUNT         1154 a         1368 c         1434 g         1280 t
ORIGIN      
        1 gagtattgcg agatgatgcc gttggacgcc gttcctgccg tgtagctgaa ggtgtagccg
       61 ctcttggtcg ggctggcggt gccgagcaag ttgtcaacca agtccgcagc cgtagatgac
      121 ggtgtagtcg cgggcccaag ggccgacaag tccgtggctg ggtccgttcc gtacgtggtc
      181 gagtaagaaa cgatggcagt gttatacgtg cgcagcgagg cgacagcaga cgcttcgttg
      241 gcagccatct tcgaacgcag caagttcggg atcgcgatag ccgcgatgat caagatgatc
      301 gcgacgacga tcagcaattc gataagtgag aaacccttct gtgtgtcgtt acgcattcag
      361 ttacgctcct tgagaatgtc tttcgtgaag tcgatttagt cccacaccaa aaacatttgg
      421 ctaaatgcaa aataattgcc aaatcacaaa acgcaaaata gggatgctaa caatgagtac
      481 aaattattga tatggaagta tttaccggag ccgacgccta gtctatttga ttggatttcg
      541 ccgtcgatac gttccgtttc atcagtggtt tgacagaatt cgtcaaatcg cttggacgcg
      601 tagtgacctt gccgccaaaa ttcgtcagta ggttcgcagg attgcgggtg tcgcagcggt
      661 ggccggcgct aaaccaatcg acactcgatt gagaataacg gcgcgcaggt tggttactag
      721 aaaggcaaag gctgtgcacg gatcaagtag gagtcagttg ccagtttagc cagtaaaacc
      781 tagacttaga tttctggtaa gagtattggc caaatcggat ctacggcttg aataatcgcc
      841 tcataccctg actcgaatga tggacttcac aaaacggctg tttagttccg cgctgttcat
      901 caccatctcc gcgtttatca tgcgcatggc ggtgctgatc tattcgtggc atgcaacgct
      961 tataccggcg cgctactttc tgccttacgg atatgagttg gggcacgtgg caaaggccat
     1021 cgcctcggga caagggtttt cttctcctct gcgatttgtg gataccggac cgacggtgtg
     1081 gttcactccg atttatcctt atttggtcgc aggcattttc aaaatttggg gaatttatac
     1141 gaacgcgtca aagctcatta tcatgacgct gaattgcgcg tttgccacgc tgacggtggt
     1201 tcctatttat tgcagcgcgc aaagaacctt cggtaaagcc gtagctgttg gggcatcgtg
     1261 gacttgggta tttttgccca cggccctgtt ttttcccatc acgtggatct gggatacagc
     1321 gatggcggcg ctctttctga gcctgatttt ctgggcaacc ctggtcgtac gcgagaaaac
     1381 aactgtcttg ccgtgggcag gctacggcgc gctgtgggcc acaggagtct taataaatcc
     1441 gtcgatgctc tcgctgctgc catttctggc gggctgggca ctttgggagg cgcggcgagg
     1501 cgaggcgcaa tggcccaaat ttgcggccgc catgctgttc atttttgcgc tcgggatggt
     1561 gccatggacc gtccgaaatt accgcgtgtt cggcaaaata atcatgcttc gctctaattt
     1621 cggacttgag ttatggctgg gcaacaatcc aaacgtgccg gatttgtgga gtccctggct
     1681 gcatccaaat gacagcaggg atgaagcttt gaaatacaaa aacatgggcg agatcgccta
     1741 catggaggaa aaacagcaag aggcgtttgc ctttatgcgc acgcatccgg cggacacgat
     1801 gaatttcatt taccgtagat ttatcgagaa ctggctggcg atttccgaca gccccacggc
     1861 cttctggggt aacacccctt tttacatccg ggcatttttt gtattgaacg tgattctgtc
     1921 cttattcaca tttgtgggcg tcctgttcgc ctaccgattt cgaattccgg aagcgtttcc
     1981 ctatgcgatg gtgccgctca ttttcccgat tatcttttat ataacgcacg cgtctctgcg
     2041 ctatcggttt ccgatggatc cgatcatgat gatcctcgcc acgtacggcg tggcaagcgt
     2101 ggttcgcttg ctgcgaggaa aatctgctca tttatcggac tccgtgtcgc ccgagacttc
     2161 cctgccgacg gcatgaccca acaataccga ggcggcagca ggacgggctt cagcttgtct
     2221 gcttttgaat ttcgcaagcg tgaaggaatt caaaagaaaa caggctgaag cctgttctac
     2281 ttgcgggcaa ttcatggtga gaggcggcga ggttaccgga tattgtattt tttggagtaa
     2341 tggcggaaag accggtacgt catttttagc aaatctgctg cgtgggtgcc gataccatcg
     2401 cacgcctgca atgcggaaag aatgtaggcc ttttccattt cctggacgga cttttcgagg
     2461 tcgatgccgc cttccggaat attgaaatgg ccgttgctgg tggaggcgtg gttatggttg
     2521 tcgtgcgtaa cgcctcgctg aatcttctca ggcagcacgg ccgtcgagat catggttccc
     2581 gtttcgaggg cgacacaccg ctcgagcgtg ttttcaagtt cgcgaacgtt cccgggccag
     2641 tcgtaggctt caaggagcgt catggtagcg ggcgtaatgc cctcgatcgg cttgtccatg
     2701 accttgcgga agcgctccag gaacgatcgc gccatcagcg gaatgtcttc gcggcgttcc
     2761 cgcagcggcg gcaaatgaat gggaatgacg ctcaagcgat aaaataaatc ctcgcgaaac
     2821 tctccggatg cgatggcctt gtccaggtcc ttgtttgtcg ctgcgatcac gcgcacatcg
     2881 atttcaaact cttcattgga gccgacggga cggactttgc cttcctgcag aacgcgaaat
     2941 aatttcacct gcatgggcag gctcatgttg ccaatttcat ccaggaaaat tgttccgccg
     3001 tgggcgccgc gaaatagtcc ctggcgattt tccgtcgctc cagtgaacgc gcctttcaag
     3061 tagccgaaca attcagactc gagaagcgtt tctggaaacg ctccgcaatt gatggtgatg
     3121 aatggttcct tgctgcgagc gctattttcg tggatggcgc gggctactag ttctttaccc
     3181 gtgccggttt cgcccgtaat caggacgcga ctggtttgcg gcgcgacggt ggtaatcatg
     3241 tcaaaaatcg cgcgcatctt cgggctgtgg ccgatgatgt tgtcttgacc ggtgagccgc
     3301 cgcaattccc tgcgcagata acccgcttcg tgcttcagct tgagcgtttc ttccacctgg
     3361 cgaaccgcac ggcgcagttg gtcgaccagg tcgtgatcct tgatgacata gcggtccgcg
     3421 ccagcattaa tcgccgcgat ggccgtttcc atggtcggca cgccggtgat cagcatgaaa
     3481 taactggagg gcgaaatttc gcggcaaaat ttcagcaatt ccacgcccgt ggtgtccggc
     3541 atgcggatgt ctgaaatgac gatgtcaaaa atactggact cgagcttgcg gcgggcggct
     3601 tctccgttca acgccacttc cacacgatgc ccctctttgc gaaaagtaat ttccagcaat
     3661 tcgcaaatag accgttcgtc atcgaccact agaatatgag ccattcaaac gcttccttcc
     3721 aaaacctgcg cgtcaagcga cgcgtggtaa atcgattgtg aactccgcgc ctttatcttt
     3781 ttcggaaaaa acgctgacac gcccgctgtg cgcctggacg atctggtaca cgatggctag
     3841 tcccaacccc gtgccgccca caaaattgga ctgcaaaggc tcaaatattt tttggcgctg
     3901 ccgcgggtcg agacccatgc ccgtatcgcg aaaacggatg cgcacccaga agggcgctgc
     3961 ttccagcgaa acggtaagaa ttccgccgtc gggcatggcg cgcatggcgt tgtcagacag
     4021 attccagaac acctgcttga tgcggttgcg atcgacgcgc acacgcactt ctttttcagt
     4081 aaacgcgcgc tcgatcttgt atttcccgtc gacttgtgga tttcgctcca gcagggtgag
     4141 cgtttcatcc agaatggtgg agacgtcctc ttccgaaaat tcgtatgttt tttcgcgtga
     4201 ataattcaga aattcggtga tgattttgtt caaacgctcc gattccctgc tcacgattcc
     4261 caccagatgc tgttcgtctt cctgtaaggg aacgagccgg cccaattcct ttacggctcc
     4321 ggccatggcc gtcagcggct ggcgaatttc atgtgctatg gccgccgaca atcgtccgag
     4381 agccgccata cgctctttgg tcgcgacttc ctgttccagg cgcttcaggt ccgtgaggtc
     4441 ctgaaaatta aaaacgtaac cggaagtgcg gcgttcgccc gttcgcaagg gggaaacgga
     4501 gatgccaatg aagcgctgct gcccatcggg cgtcatcagg tccacttcgc ggcgctgcgg
     4561 aagtttcccg tcttccgaca catcgcccgg aagccagaac gagggccaca atttttgaac
     4621 gttgctgcgc ttgacattgc ggaattggca gccggtaatt tcttcacccg tgcgattcaa
     4681 gagcagaatc ctgccttcca gatccgtggt caacaggccg ccgcgcatgg agtggataat
     4741 gtcttgattg aacgcctgca agtcttgcag ctgcccgcgc ttcacttcca actcttcact
     4801 atggcgccgc agcgtctgtg caagaaggct ggaaagatat gccacggcca tgaaataaaa
     4861 taaattgctg aagatccagg tctgcagctg gcggctgctg ggcaccgcaa tttgcatgcg
     4921 agggatgatg ttgtagaaaa taagctcggc gagcccgcca atcaaaacaa aactgaagcc
     4981 tgcgactgtg aatgtaactt ttcgcgaaaa caggatgctg gcgacgatga tggccagcgg
     5041 gtagagccag ataaaatagc tgtcctgcgt gccggtcaaa taaatcaacc ctgtgactat
     5101 cagcagatca aaaattaatt gaacgccggc gtgccacttg gcgtcgggca tccaccgcgt
     5161 cagaatcgaa tacacgattg ccatcgtgta ccacaggatc atcaggggga tgaaatagcg
     5221 cgtcgacacc tgcagc
//
LOCUS       DASZMB010000161        12752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1287108, whole genome shotgun sequence.
ACCESSION   DASZMB010000161 DASZMB010000000
VERSION     DASZMB010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 12752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12752
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287108"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="VG272_07300"
     CDS             <1..430
                     /locus_tag="VG272_07300"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWF13525.1"
                     /translation="GGIGQLHAAGAALEKLEVINQPVASIAKQEEIIYVLGQEDQPIR
                     LEHHSPILHLIQQIRDETHRFAVGFHRQRRSKRTIQTGLEDIPGVGPRTAQKLLRNFG
                     SITQLRSASMEQIAKVVPKGIAERVVAHLAGNKTTTVHSA"
     gene            490..783
                     /locus_tag="VG272_07305"
     CDS             490..783
                     /locus_tag="VG272_07305"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HWF13526.1"
                     /translation="MADNAGSKVSYFLVGLGIGALVGILFAPKSGEETREYLSQKADE
                     GREYAQRKAQELRERAEDLVERGKSVASRQKDNLSAAVDAGRDAYMREKSKAQ"
     gene            803..1318
                     /locus_tag="VG272_07310"
     CDS             803..1318
                     /locus_tag="VG272_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HWF13527.1"
                     /translation="MESWMPLFVAVIAIAVVLQSIFLIAMFIQMRRTAARVEQVVTDI
                     SGKVAPLISSVQVLVDDISPRITGIVADASEITRLARGEVQKVDRIVSEALERLRMQL
                     IHVDHILTGAIETVEQAGSQIRQTVWGPVVKATAMLRGVQAGIDFLRNVRGRRDGGDI
                     PADQQDEGMFI"
     gene            complement(1394..2515)
                     /gene="mnmA"
                     /locus_tag="VG272_07315"
     CDS             complement(1394..2515)
                     /gene="mnmA"
                     /locus_tag="VG272_07315"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HWF13528.1"
                     /translation="MELTTNQKPMTAVAMSGGVDSSAVAAILKDQGCEVVGLTMQLWN
                     QRRLPELDRDGSHGQRCCSIDDVYDAKRVAQHLGIPHYVVNYEQQFEETVVQPFVEEY
                     LAGRTPVPCTMCNNHVKFDPLLVTSRQIGAERMATGHYARIRHNEGTGRYELLRAADA
                     SKDQTFFLFGLKQEQMSRTDFPLGELDKTAVRDIARRAALPVAEKPDSQEICFVPSGS
                     YVKFISAYLGERGETLSEDEGDLVTTSGEVIGRHHGVHNYTIGQRKGLGVAAGKPLYV
                     VELDRTANRVIVGDDSDLLTNICEVRDVNWISFASLEEPMRATVKIRHRHAPAAATVD
                     PIDETSARIIFDEPQRAVTPGQAAVCYSNDVVLGGGWIK"
     gene            complement(2564..3736)
                     /locus_tag="VG272_07320"
     CDS             complement(2564..3736)
                     /locus_tag="VG272_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HWF13529.1"
                     /translation="MKRIYFDHNATTPVAPEVLAAMMPHLTEEYGNASSLHAFGQNAR
                     EAVEQARVSVAALLNARPVEIMFTSGGTESINHAILGAVAAVPGNSKHVITSAIEHVA
                     GLDPCKALAKSGVDLTILPVDREGLISPNEVLRAVRPETVLINLMLANNETGTIEPIA
                     EIGKIAAEKGIVLHTDAVQAAGKIPIDVETLGVDLLSISAHKFNGPKGIGALYIRKGT
                     PLSPLMYGGHSERDRRPGTEDVAAIAGMGRAANLAHRSVSQESERIRELRDRLEHGLL
                     DRVPQSCVNGARAPRVPNTSNLTFPFIEGEAMVIALDLKGIACSTGAACSSGAVEPSH
                     VLMALGLAPEDARATLRLSLGRQNTPEEIDFALEIIPSVIERLRRLSPTYKNPAIA"
     gene            4058..4849
                     /locus_tag="VG272_07325"
     CDS             4058..4849
                     /locus_tag="VG272_07325"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HWF13530.1"
                     /translation="MPDPIITLTTDFGTSDHLVGTVKGVILNINPACRIVDINHSVVP
                     FDLLDGALSIANAYSFFPARTIHVVIVDPGVGTDRRPILVTAGNQFFVAPDNGVLSMI
                     YERESCTVRHITAEHYFLNPISPTFHGRDIFAPTAAWLSKTFQTEAFGEAILDFVRFT
                     TPKAKANGQGVKGVVLRVDVFGNLMTNLTAENIPAGALQGGPIRLAVNGKKISQFAPT
                     FASGTPGEPIAVVGSTGFVEIAVNRGNAARTLGVGRGAEVILEND"
     gene            complement(5039..5914)
                     /locus_tag="VG272_07330"
     CDS             complement(5039..5914)
                     /locus_tag="VG272_07330"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF13531.1"
                     /translation="MKPTSQLRQFLRTSGLGRWGFAVTALLIILAVFAPWIAPANPSA
                     QSLPNRLLSPSAHHWMGTDELGRDVLSRTLYGARVSLLVGISVVLGAGIVGLALGSIS
                     GYFGGAFDRFINIVLINSFLSFPGILLAIAFAAFLGPGLDKVIMALVITGWAGYARLA
                     RAQVLKVREMEFILAARSLGASHTRILLKHLLPNTLQPVLIQATIGMAGAILAESTLS
                     FLGLGVVAPTPSWGAMLNDARNHLFDAPHLVIFPALGIMAAVLAFNLLGDALRDWLDP
                     RTRAFLITMDLPPEV"
     gene            complement(6090..7010)
                     /gene="nikB"
                     /locus_tag="VG272_07335"
     CDS             complement(6090..7010)
                     /gene="nikB"
                     /locus_tag="VG272_07335"
                     /inference="COORDINATES: protein motif:HMM:NF045470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease"
                     /protein_id="HWF13532.1"
                     /translation="MIRHFLQRLLYTLPALWLILTMVFFMIHIVPGDPVLQMLGQDAR
                     VEDLAQLRHTLGLDQPLGVQYVRYFEALARGDWGRSMRYSAPVRPIVLQRFPATLELS
                     LAALAICIAIAIPAGVFSARRRGSQADRATSFFTLLGLSVPNFALGPILILIFSIEMG
                     WLPVSGRGGIVHLILPAATLGAALAAILTRMVRGSMIEELSSDYVRTARAKGITETSV
                     LFRHAFPNALIPVITIVGLQFGSLLAGTIVTETIFSWPGIGRLAVQAIAARDYPLLQG
                     CILIIALSYVGVNLLTDLIYAMVDPRVRLQ"
     gene            complement(7007..8413)
                     /locus_tag="VG272_07340"
     CDS             complement(7007..8413)
                     /locus_tag="VG272_07340"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWF13533.1"
                     /translation="MIGLRLLGLFALVAVNGFFAAVEFSLVAVRHSRVRQLVDQGRFR
                     AKIVLDLIGKLDRVVSGVQVGITLTSLGLGALGESTLASILSPFVANIPGAHRVVLIH
                     AVSLAVAFLFLTILHVVLGELVPKSMSLQRAERVALLVAPPFQWYLEMFRPAISLLDG
                     ISRWVLSGLGFSGSTSHTQVHSAEELQIQIQQARERGLVGRDEEKFILGAIELDQMQV
                     RELMVPRPDVHFVTADASLDDVMRIFVTTQRSRLPVFEGAGEQVLGYIHIKDIVWVLL
                     DRERRAEEGWTAAEFSVRSMLHEIAIVPESKPVSEMLLEFRTHHGWMAMVVDEFGTIL
                     GLLTLEDILEQLIGEIHDEFDVVERPIVVGQGADVAMIFDASLGLRDLESQYNIVLPE
                     DRSYATVGGFVLARLGFIPRGGESFDFTGYRFTVVEMDRRRVARVKIQRLNVAAERSG
                     SAPLPEAKPATTKVLKQK"
     gene            8625..8906
                     /locus_tag="VG272_07345"
     CDS             8625..8906
                     /locus_tag="VG272_07345"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HWF13534.1"
                     /translation="MTTVNPASSKKPEPGKKPDFENSLARLEEIVGKLENANLSLDEA
                     MKLFEEGVQLSRDCQKYLGQAEGKVEILLKKAGGEPIAEPFDPEAEEES"
     gene            8946..9827
                     /locus_tag="VG272_07350"
     CDS             8946..9827
                     /locus_tag="VG272_07350"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF045485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HWF13535.1"
                     /translation="MTLPTFFEEDRAAVDAALDWLMPGENTQPASIHKAMRYSVQAGG
                     KRIRPILCLESARIFSESVASVLPVACALEFIHTYSLIHDDLPALDNDDLRRGKPTCH
                     KKFNEATAILAGDALLTLAFETLADAPIEPVRRVAILSHVAASAGTVNGMVGGQVADI
                     EAEGRSIQPEELEYIHRSKTAALIRASVVAGAISGGGEEKDVEHLRRFGETIGWAFQV
                     VDDILDVEESSASLGKTAGKDVAQKKATYPSIYGLEKSRQFAKELEERAMAEIEPYGE
                     RASRLRELADMIVHRRA"
     gene            10091..10843
                     /locus_tag="VG272_07355"
     CDS             10091..10843
                     /locus_tag="VG272_07355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HWF13536.1"
                     /translation="MPPKQKIAKKRLDRLVVERGLAESREKAQAMILAGEVRVNGARS
                     DKAGAQIGEDVRLEVSGASAKYASRGGFKLEGALEDFSVDVSGKICLDVGASTGGFTD
                     CLLQHGAKRVYAVDVTPQQMAWRLVQDSRVTQIKANARNLRPAEIPGPVDFVTMDVSF
                     ISVAKVLPAVVAAAGLDAEYMILVKPQFELDRGDVGSGGIVRDAALHDRAIERVRQAA
                     LAAGLKVKGIRASHLTGAEGNQEFFLCAGKGE"
     gene            11047..11910
                     /locus_tag="VG272_07360"
     CDS             11047..11910
                     /locus_tag="VG272_07360"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HWF13537.1"
                     /translation="MIKTVGIISRPRREDVALVVPPLVNWLQAHGADVVCDSETSDCI
                     QPLAAQTRKREDIPGSTDLLIVLGGDGTLLSAARLAAERKVPVLAVNLGGLGFLTTVP
                     QDEIYSILEELFSGLHRVSERVMLDAEIVRGGTVIRRQIALNDAVLNKAALARIMDLE
                     LRVDGEYVTTYKSDGLIISTPTGSTAYSLAAGGPIVYPSVEAFVVSPICPHTLTNRPI
                     VIPDSATIEIDFKAEDDAVFLTLDGQIGIELIRGDHIRVRKATNKLMLVRPAKKTYYQ
                     ILRSKLKWGER"
     gene            11966..12583
                     /locus_tag="VG272_07365"
     CDS             11966..12583
                     /locus_tag="VG272_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13538.1"
                     /translation="MPTNLFRPIGLRELELLWDSGMKEFPQRLENQAIFYPVVNLEYA
                     RQIARDWNTPDAKSGYSGFVTKFEVSSTYLSKFELHTAGSAAHREYWIPAKELSVFNK
                     AINGQISVEEGFFGSEFVGYREEENGFKNFNAVEQFSALLKMADQDDFSSQVSAHRKV
                     IFMNCLFWLNTDISNLPCTKEQRDTFLTALVNAWEADSIGVPLPI"
BASE COUNT         2979 a         3428 c         3532 g         2813 t
ORIGIN      
        1 tggcggtatc ggccagctcc atgccgcggg cgcggcgctc gaaaaactgg aagtcatcaa
       61 ccagcctgtg gcgagcatcg cgaagcagga agaaattatt tacgtcctgg gacaggaaga
      121 tcaaccgatc cggctcgagc accactcccc tattcttcat ttaatccagc aaatacgtga
      181 tgagacgcac cgcttcgccg tgggttttca ccgccagcgg cgatccaaac gcacgattca
      241 aaccgggctg gaagacattc caggagtggg cccgcgcact gcccaaaaat tgctacggaa
      301 tttcggaagc atcacgcaac tgcgctccgc aagcatggag caaattgcca aggttgttcc
      361 taagggcatt gctgaacgcg tagttgcgca cctcgctggt aacaagacca cgacagttca
      421 cagcgcgtag ttccattgat tgcttctctc aaccccgtgt gctactttcc ataaggaaag
      481 gagtatgtca tggctgataa tgctggatca aaagttagct attttctcgt aggtctcgga
      541 atcggggcgc ttgttggaat tttgtttgcg ccgaaatccg gtgaagaaac acgcgagtac
      601 ctgtcacaaa aggccgatga aggccgtgaa tatgctcaac gcaaagccca agagcttcgc
      661 gaacgcgcgg aagacctggt tgagcgcggc aaatccgtcg cttcacgcca gaaggataat
      721 ttgtccgcgg ccgtagatgc aggtcgcgac gcctacatgc gggaaaaatc gaaagcacag
      781 taatcacgac gaggataagc gaatggaaag ctggatgccc ttgttcgttg ctgtcattgc
      841 gattgccgtt gtcttgcagt cgattttctt gattgcaatg tttattcaaa tgcgccgtac
      901 ggctgcgcgc gtcgagcaag tcgtgacgga tatcagcggc aaggtagccc cgctcatttc
      961 gtcggtacag gttttggtgg acgacatttc cccgcgcatt acgggcatcg tggcagacgc
     1021 ttccgagatc actcgcctgg cgcgtggcga agtgcagaag gtcgaccgca tcgtgtccga
     1081 ggctctcgag cgcctgcgca tgcaactcat ccatgtggac catattctga caggcgcgat
     1141 tgaaaccgtg gaacaagccg gatcgcaaat tcgccagacc gtttgggggc ctgtggttaa
     1201 ggcgacagcg atgcttcgcg gagttcaggc cggcattgat tttctgagga atgtgcgagg
     1261 acggcgcgat gggggggata ttccagccga tcagcaggat gagggaatgt ttatttagtt
     1321 gggttttacc gcatctggac gttgcgaaaa aatccgcctc tgaaggggcg gaccacagtt
     1381 tccgctacaa aatctacttg atccaacctc cacctagaac aacatcattt gaatagcaaa
     1441 ccgcggcttg tcccggagtg acggctcttt gtggttcgtc aaaaatgatg cgcgcggaag
     1501 tttcgtcaat aggatcgacc gttgctgcgg caggtgcatg gcgatggcgg atttttactg
     1561 ttgcacgcat gggttcttcc agacttgcga acgaaatcca attcacatcg cgtacttcgc
     1621 aaatatttgt cagcagatct gagtcatcgc ccacgatgac gcggttggcg gtgcgatcca
     1681 gttccacgac gtaaagaggt ttcccagcgg caacgcccag ccctttgcgc tgccctattg
     1741 tgtaattgtg gacaccgtga tggcggccga tgacttcgcc gctcgtggta accagatcgc
     1801 cttcgtcctc cgaaagcgtt tcgccgcgct cgcctaaata ggcgctgatg aacttgacat
     1861 agcttccaga tggtacaaaa caaatttcct ggctatcggg cttttcggcc accggtagcg
     1921 cggcacggcg cgcgatatca cgcacggccg tcttatccaa ttcgccgagc ggaaaatcgg
     1981 tgcgcgacat ttgttcctgc ttcagtccaa aaagaaaaaa tgtttgatcc ttggaagcgt
     2041 ccgcggcgcg cagcaattcg tagcggcctg tcccttcgtt gtggcggatt cgcgcgtaat
     2101 ggcctgtggc cattcgctcc gccccaatct ggcgcgaggt gaccagcagc ggatcgaact
     2161 ttacgtggtt attgcacatg gtgcacggca ccggagtgcg gcctgcgaga tattcctcga
     2221 cgaacggctg caccacggtt tcctcaaatt gctgttcgta attcaccacg tagtggggaa
     2281 tgccgagatg ctgggctacg cgcttggcgt cgtacacatc gtcaatcgag cagcagcgct
     2341 ggccgtgcga gccatcgcga tcgagttccg gaaggcggcg ctggttccac agctgcatgg
     2401 tcaggccaac cacttcgcag ccttggtcct tgaggatcgc ggcgacagcc gaactgtcca
     2461 caccgccgct catcgcgaca gccgtcatgg gcttctgatt tgtagttaat tccatttgta
     2521 ttggggtcta atgccgaacc cgcagtccca ttatagcctt aaatcaggcg attgcaggat
     2581 tcttataggt gggagaaagc cgtcgtagcc tttcaataac cgaagggata atttcgagcg
     2641 cgaagtcaat ttcttccggc gtattctggc gtccgaggct caggcgcagc gtggcgcgcg
     2701 cgtcctctgg agcaagtccc agcgccatca atacatgcga aggttctaca gcccccgaag
     2761 agcaagccgc gccggtagag cacgcgattc ccttcaggtc gagcgcgatg accatcgctt
     2821 cgccctcgat aaaggggaaa gtcaggttgg aagtgttggg tacccgcggt gcgcgcgctc
     2881 cgtttacaca ggattgcggc acccgatcta gcaagccgtg ctctaagcga tcccgtagct
     2941 cgcggatgcg ttcactttcc tgagagacac tcctatgcgc gaggttcgct gcccggccca
     3001 tgcccgcgat tgctgcgaca tcctcggtcc ctggccgcct gtcacgctcc gagtggccgc
     3061 cgtacattaa aggtgagagg ggcgttccct ttcgaatgta tagcgcgcct attccctttg
     3121 gcccattaaa tttgtgggcg gaaatggaaa gcagatccac gccaagcgtt tccacatcga
     3181 tggggatttt ccctgcggct tgcacggcgt ccgtgtgaag cacgattccc ttttcggcgg
     3241 caattttgcc gatttcagcg attggctcga tcgttccggt ctcgttattt gcgagcatga
     3301 ggttgatgag cacggtttca ggacgaacgg cgcgtaaaac ttcgttggga cttataagtc
     3361 cttcgcggtc cacgggcaaa atggtgagat ccacgccact ttttgcgagc gccttgcaag
     3421 gatcgaggcc tgcaacatgc tcaatcgcag aggtaataac gtgcttcgag tttcccggca
     3481 ccgcggcaac cgcgcctaga atcgcatggt taatggattc ggttccgcca ctcgtaaaca
     3541 tgatctccac ggggcgtgca ttgagaagcg cggcaaccga cacccgggcc tgctcgactg
     3601 cttcgcgcgc attctggccg aaagcatgga gcgagctcgc attgccgtat tcctcagtga
     3661 gatgcggcat catagcggcc aacacttcgg gcgctacggg ggtcgtcgcg ttatggtcaa
     3721 agtaaatgcg cttcatgtcg tgctttctgt tattcgaaac caaatgtacg attggcactg
     3781 aatgcagaat atcgttcgaa caaggagcgg tcaaggtggc gttccgcggc ataggcttca
     3841 gcctgtggcc tttggccctt ttgagcgctt caactcatag gggttcgtct ttgcagggac
     3901 gaacccacgg agctgaagcc tatgccactc cactgcaata gccagtcggg cacatcgcct
     3961 tgactggtgg aagggccttc gataggttga taaaggagta aaactcgacg cgctcgcggt
     4021 tcattttcgc gcgcgaaacg gaaaaacgga gacccacttg cccgatccca tcatcacgct
     4081 cacgaccgat tttggcacga gcgaccatct tgtcggtacg gtgaagggcg taatcctgaa
     4141 tattaatcct gcctgtcgaa tcgtggatat taaccatagc gtggttccct tcgatcttct
     4201 cgatggggcg ctgtcgattg ccaatgccta ctcttttttt ccggcccgta ccattcatgt
     4261 ggtcatcgtg gatcccgggg tcggaacgga ccgccgcccc attctggtga ccgcgggtaa
     4321 tcagtttttt gtggcaccgg ataatggtgt tctctcgatg atctatgagc gggaatcctg
     4381 cacggttcgc catatcacag cggaacatta tttcctaaac ccaatcagcc cgacatttca
     4441 tgggagagat atctttgcgc cgaccgcagc atggctttcc aaaacttttc agacggaagc
     4501 attcggagaa gctattttag attttgtgcg attcaccacg ccgaaagcga aagccaacgg
     4561 acaaggagta aaaggcgttg tgttgcgcgt cgacgtgttt gggaacctga tgacgaattt
     4621 gacggcggag aatattcccg cgggcgctct tcagggtggg ccaatccggc ttgccgtgaa
     4681 cggcaaaaaa atcagtcaat tcgcgccaac atttgcgtcg gggaccccag gtgagccgat
     4741 tgccgtggtt ggatcgacgg gcttcgtgga aattgcggtg aaccggggaa atgccgcacg
     4801 tactttgggt gttggacgcg gcgcggaagt tatcctcgaa aacgattgag ctcgattttt
     4861 ctcgacagcc gattctgaag cgctttaagt agcacgggct tcagagtctg tgtgagaact
     4921 gcagaatctt tgtgggtcgt ggcttcagcc acgacataag tcctacaaaa taagtgcggc
     4981 tttagccgct gaagcttcaa ttttgcagtt ctcacacaga ctctgaagcc cgcgctactt
     5041 aaacttcggg cggcaaatcc atcgtgatca ggaaagcgcg tgtcctggga tccagccaat
     5101 cgcgcagggc atctcccaac aaattaaagg ccaggactgc cgccatgatt ccgagcgcgg
     5161 gaaatatgac gagatgtgga gcgtcaaata aatgattcct cgcgtcgttc aacatggctc
     5221 cccaacttgg cgtgggcgcg acaacgccta gtccgagaaa actcaaagtg gactccgcca
     5281 ggattgcacc cgccataccg atggttgctt gaattaagac tggctgcaga gtgttgggca
     5341 agagatgctt gagcaagata cgtgtgtgcg atgctcccag tgaacgagcc gccagaataa
     5401 attccatctc gcgcactttc agtacttgcg cgcgggccag gcgcgcgtat cccgcccatc
     5461 ctgtgatgac caaagccatg atgactttgt ccaagcccgg gcctaagaat gccgcgaatg
     5521 ctatcgctaa caaaattccc ggaaaagata aaaaggaatt gatcagcaca atgttaatga
     5581 agcggtcaaa ggcgccaccg aaataaccag aaatcgagcc aagcgccagg cccacgattc
     5641 ctgctcccag tactacggag atgcccacga ggagcgagac gcgcgcgcca tatagagtgc
     5701 gcgacaggac gtcacggccc aactcatccg ttcccatcca atgatgcgcg ctgggcgaaa
     5761 gcaaccggtt ggggaggctt tgcgccgagg ggtttgcggg agcgatccac ggggcgaaga
     5821 cggcaagaat gattagaagc gcagttactg caaagcccca gcggcccagg ccgctggtgc
     5881 ggaggaattg ccgaagttgt gatgtgggtt tcatatttat ttcttcagca ttttcatcta
     5941 cttcttttgt tatttgccaa taccacaggc cttcagcctg tgggcgttgg tccatgctgg
     6001 gtccaaccgt tcgcactgaa gtttgtagcc actaaaccct cgcctctgaa gaggtgagct
     6061 acacaagcca gccgcaaaaa ctcggcgggt cattgcaggc gcaccctcgg atccaccatg
     6121 gcatagatca aatccgtcag cagatttacg ccgacatagg acaaggcaat gatgaggatg
     6181 cagccttgca ataacggata atcgcgcgcc gcgatagctt gcacggccag tcggccgatc
     6241 ccaggccacg agaaaattgt ttcggttacg atggtcccgg ccagtagcga accaaattgc
     6301 aatccaacga tcgtgatgac cgggatcaga gcgttgggaa aggcgtggcg gaacaagaca
     6361 gaggtttcgg tgatgccttt tgctcgcgcc gttcgcacat agtcgctcga cagttcctcg
     6421 atcatcgaac cgcgaaccat acgcgtaaga atcgctgcga gcgctgcgcc gagagttgcg
     6481 gcgggtaaaa ttaagtggac gattcctccg cgtcctgaca cgggcaacca tcccatttca
     6541 attgaaaaaa ttaaaattaa aattgggccc agcgcgaagt tgggaacgga aaggcccagc
     6601 agagtgaaaa atgaagtggc gcgatccgcc tggctgccgc ggcgccgcgc ggaaaaaact
     6661 ccggccggaa tagcaatcgc gatacatatg gcgagagccg caagcgacaa ttccagagtt
     6721 gcgggaaacc gctgcaacac aatggggcga actggcgccg aatagcgcat cgagcggccc
     6781 caatcgccgc gcgccaatgc ttcaaaatat cggacatatt gcacgccaag cggctgatcg
     6841 agccccagcg tgtgccgtag ttgtgcgaga tcctcaacac gcgcatcctg gccgagcatt
     6901 tgcaatacgg gatcgcccgg cacgatgtga atcataaaaa aaaccatcgt gaggatcagc
     6961 cagagagccg gcaacgtgta gagaaggcgc tgaaggaaat ggcggatcat ttttgtttga
     7021 gcaccttcgt cgtggcaggc tttgcttcag gcaacggcgc tgagccactg cgttccgcgg
     7081 ccacgtttag ccgctgaatt tttacgcgcg ccactctgcg acgatccatt tccacgacgg
     7141 tgaaacggta tccagtgaaa tcaaaacttt ctccgccccg cgggatgaag ccgaggcggg
     7201 caagaacaaa accgcccacc gtcgcatacg agcgatcttc cggcagaaca atgttgtact
     7261 gcgattcgag atcgcgcagg ccaagcgaag cgtcgaaaat catggcgacg tccgccccct
     7321 gaccgacaac gatggggcgc tcgacgacgt caaattcatc gtgaatctcg ccgatcagct
     7381 gttccaaaat atcttcgagc gtgagcaatc caagtatcgt tccgaactca tcaaccacca
     7441 tagccatcca gccatgatga gtccgaaatt ctaggagcat ttcactgact ggcttgctct
     7501 ccggcacgat ggcaatttcg tggagcatgc tgcgcacact gaactcagcg gccgtccagc
     7561 cttcctccgc gcggcgttcg cgatcaagca gcacccacac aatatctttt atgtgaatgt
     7621 agccgaggac ctgctcgccc gctccctcaa atacgggaag ccgcgaccgc tgcgtcgtga
     7681 cgaagatgcg catgacatca tcgagcgaag catcagccgt cacgaagtga acatcggggc
     7741 gcggcaccat caattcccgc acttgcatct gatccagctc aattgcgccc aggatgaatt
     7801 tctcctcatc ccttcccacc aggccgcgtt cgcgcgcctg ctgaatctga atttgcaatt
     7861 cttccgcgga gtggacttgc gtgtggctcg tactcccgga aaatcccaag ccgctcagca
     7921 cccaacggct aatcccatcg agcagactaa ttgccgggcg aaacatttcc agataccact
     7981 gaaaaggcgg agcgaccaac agggcaacgc gctccgctcg ctgcaggctc atgctttttg
     8041 gcacaagttc gcccagcacc acatgcaata tcgtcagaaa aagaaaagca acggcaagag
     8101 aaaccgcgtg aatcaggaca actctgtgtg cgccgggaat atttgcgacg aacgggctca
     8161 agatggaagc gagtgtggat tcgccgagcg cgccgagccc caggctggtg agcgttattc
     8221 ccacctggac gccggagact acgcgatcca gcttgccgat taagtccaaa acaattttcg
     8281 cccgaaatcg accctgatcg acaagttgac gaacgcgcga atggcgcacc gcaaccagtg
     8341 aaaactcgac tgccgcgaaa aatccattca cggccaccag agcgaacaat ccgaggagtc
     8401 gcaacccaat caagccggca agttcctccg aaggctcatt ctagcagccc cttcctccca
     8461 tctgaaagga ccgagcacgc gcatgatacg caagagtggc tgtcgaatcg atcccagaaa
     8521 tcattccgca gcataaagcg gttgtttcgg ttctcgggcg tgcattagaa tgttgtcttc
     8581 gtttgccaac ttttttgatc gctgctttac tgaaatcgtg aactatgaca accgtgaatc
     8641 ccgcatcgag taaaaaaccg gagccgggca agaagccgga ctttgagaat tcccttgcgc
     8701 gcctcgaaga aattgtgggc aagctcgaga atgcgaattt gtctctcgac gaagccatga
     8761 agctgtttga agaaggtgtg caactttcgc gcgactgcca gaagtatctg gggcaagcgg
     8821 aaggcaaagt ggaaatcctg ctcaagaaag cgggcggcga acctattgcc gagcccttcg
     8881 atcccgaagc cgaggaagaa tcctaatccg caccggcaaa tgagcggcca agccgcattc
     8941 ctctgatgac ccttcccaca tttttcgaag aagaccgggc cgcggttgac gctgcccttg
     9001 actggctgat gcccggtgaa aacacgcaac ctgcttccat ccacaaagcg atgcgttaca
     9061 gcgtgcaggc cggagggaaa cgcattcggc ctatcctgtg cctcgaatcg gcgagaattt
     9121 tttccgagag tgtggcttcc gttttgcctg ttgcgtgtgc tcttgaattt attcatacgt
     9181 actcgctgat tcatgacgat ttgcctgcac tcgataatga tgatttgcgg cgcggaaaac
     9241 cgacttgcca caaaaaattt aatgaggcca cggctatcct cgccggcgat gcattgctga
     9301 ccctggcatt tgaaacgctt gctgatgcgc cgatagaacc ggtgcgccgc gttgccattc
     9361 tttcccacgt tgccgcatcg gcgggcacgg tgaacgggat ggttggcggc caggttgctg
     9421 acatagaggc ggagggccgc tctatccagc cagaggaact cgaatatatt caccgctcaa
     9481 aaaccgctgc gttaatccgc gcctctgttg ttgctggagc aattagcggc ggcggtgaag
     9541 aaaaagacgt cgaacattta aggcgttttg gcgaaaccat tggttgggcg tttcaagtgg
     9601 tcgacgatat tttggacgtc gaagaatcgt ccgcgtcgct cggaaaaacg gccggcaaag
     9661 atgtagccca gaaaaaagcg acataccctt cgatctatgg cttggagaaa tctcgtcaat
     9721 tcgccaagga gttggaagag cgcgccatgg ctgagatcga accgtacgga gaacgagcgt
     9781 cacgcctgcg ggaattagca gacatgatcg ttcaccgtcg cgcgtaattg cgacgaagcg
     9841 ggtttctgcg cggcattgta gcctcacctc ttcagaggca gggggcttga cgtggtcttg
     9901 ctcgattctt ggaattgagg cggcgaattt cgcgtagcag cgtttaacgc tggaaacgtg
     9961 cgctgaccag ttcattttag cgttacactt tcatgtagac cctcggctct gatccttcgt
    10021 tttcactcag gacaagagag gcgagctaca atcgtcccgc gatcatcgcc ggcgatttcg
    10081 attaaacgca gtgccgccaa aacaaaaaat cgccaagaaa aggctcgatc ggctggttgt
    10141 cgaacgcggc ctcgccgaat cccgcgaaaa agcgcaggcg atgattctgg ctggagaagt
    10201 gcgcgtgaat ggagcgcgaa gcgacaaggc cggcgcgcaa attggcgaag acgtacgttt
    10261 agaagtcagc ggagcgtccg caaaatatgc gagccgcgga ggtttcaagt tagagggtgc
    10321 gctcgaagat ttcagcgtcg atgtaagcgg caaaatctgt ctggatgtgg gcgcatccac
    10381 gggaggcttt accgattgtc tcttgcaaca tggcgcgaaa cgcgtttacg cggttgacgt
    10441 gacgccgcaa cagatggcgt ggcggctcgt gcaggattct cgtgttaccc aaattaaggc
    10501 taatgcgcgc aatcttcggc cagcagaaat tccgggccca gtggatttcg ttaccatgga
    10561 tgtttctttt atttccgtcg cgaaagtgct tcctgctgtt gtcgcggcgg ccggccttga
    10621 cgctgaatac atgattttgg ttaagccgca atttgaactc gatcgcggag atgttggcag
    10681 cggaggaatt gtgcgggacg cggcgctcca cgaccgcgca attgagcgcg ttcggcaggc
    10741 tgctctggct gcgggattga aggtcaaggg cattcgcgct agccacctca caggcgccga
    10801 gggcaaccag gaattttttc tatgtgcggg gaaaggcgag taggttttcg tggcaaaggt
    10861 ttcagcctgt ggggtgcgtc cttgcaagga ctaaattcca ccggctgaag cctttgccac
    10921 gaaaatctgt ctcatctgca ccggggattt ctcgctttga caatcttcgt tgaaatcagg
    10981 gaaatagaaa tgcgcgacag atgctaaagt aagtgttcgc tttggcagcg tttcggcaca
    11041 aataaaatga tcaagaccgt cggaatcatc tcacgtccac ggcgcgaaga tgtcgcgctc
    11101 gtcgttccac ctcttgttaa ttggctgcag gcccacggcg cggacgtggt ctgcgattcg
    11161 gaaacgagcg attgcatcca gccgctcgcc gcgcagactc gcaagcggga agatatcccc
    11221 ggctccacgg atttgttgat cgtgcttggg ggcgatggaa ctttgctgtc cgcagcgcgc
    11281 ctggcggccg aacgcaaagt tccagtcctc gcggttaatc tgggcggcct cggttttctt
    11341 acaactgttc cgcaggacga aatttattcc attctggagg agcttttctc cgggctgcat
    11401 cgcgtaagtg aacgcgtcat gctggacgcg gaaatagttc gagggggaac cgtcattcgg
    11461 cgtcaaattg cgttgaacga cgcagtgctg aataaggcgg ctcttgcgcg cattatggat
    11521 ttggaactgc gcgtggacgg cgaatacgtg accacataca aatcggatgg cttgattatt
    11581 tcaacgccta ccggatcgac cgcctattcg ctcgcagctg gcgggccgat cgtttatcca
    11641 agcgtcgaag cctttgtggt atctcccatt tgcccgcaca cactgacgaa ccgccccatt
    11701 gtgattcctg attcagcaac gatcgagatt gatttcaaag cggaagacga tgctgtgttc
    11761 ctgacactcg atggtcaaat cggaatcgaa ctcattcgag gggatcacat ccgtgtgcga
    11821 aaagccacga acaaattaat gttggtgcgc ccagcaaaaa aaacctacta tcaaattcta
    11881 cgcagtaaat taaagtgggg cgaacgataa aatcgccctt cggcgttatt ttgcacgccg
    11941 aactgcggca accatcgctg acattatgcc tacaaatctt ttccggccaa tcggccttcg
    12001 cgaactcgag ctactttggg attcaggaat gaaagaattc ccgcaacgtt tggaaaatca
    12061 agcaattttc tatcccgttg tgaatttgga atacgcccgg caaatcgcgc gggactggaa
    12121 tacgccggat gccaagtcgg gctactccgg ttttgtcaca aagtttgaag tgtcttccac
    12181 atatctttcc aagtttgaat tgcacacagc cggatcggca gcgcatcgcg aatattggat
    12241 tcctgcgaaa gaactcagtg tatttaacaa agcgatcaat ggccaaattt ccgtcgaaga
    12301 aggattcttt ggatcggaat ttgtcggcta ccgggaagaa gagaatggtt ttaaaaattt
    12361 caacgctgtc gaacaatttt ccgctttgct aaaaatggcc gatcaggatg atttcagttc
    12421 gcaggtttcg gcacaccgca aagtgatttt catgaattgc ttgttttggc tgaatacgga
    12481 tatttccaat cttccatgta cgaaggaaca aagagacacg ttccttactg cactggtgaa
    12541 cgcctgggaa gcggattcca tcggcgtacc tcttccgatc tgatttttga ataattagag
    12601 atcgtctcgt aaatgacctg gacttcaagc ccggtcgccg gtcagggggc tcaagcactg
    12661 tttttcttcg catttcgatt tcgcggctga agcctcgatc cacaggtatc agggcttggc
    12721 cctgttgaca tcacaaaaaa ggctagcgaa ac
//
LOCUS       DASZMB010000162         7484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2913902, whole genome shotgun sequence.
ACCESSION   DASZMB010000162 DASZMB010000000
VERSION     DASZMB010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7484
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2913902"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..1041
                     /locus_tag="VG272_07370"
     CDS             205..1041
                     /locus_tag="VG272_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13539.1"
                     /translation="MIAIFSYSKKKSFIPPLVIPILALFASFVMLSDTFYFIGIQAQT
                     NGNMAAEFKRTSEELSSARLGGGSENKVQLEKALAYLDSAAILTLNSASSPDLEAANR
                     RLATLVSQTSPVGENYRLLKIGGSPAVYAMAINFGLGGPAAVRIYTGGTGHYSLVAQI
                     DHYAQKDFLDSDIELVSVSPADMVFVTVSGRTDDLSTGAFTAWQFNGHAVTALWNSDL
                     LQQSSYEVEGNGFRIAYCSQVDDDRPSQCLKMSRDLFRLDAGQWKRVETAELSSPKPT
                     SK"
     gene            1157..2572
                     /locus_tag="VG272_07375"
     CDS             1157..2572
                     /locus_tag="VG272_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13540.1"
                     /translation="MDRVKRFLVCMVTMLFLAAGAGAQNAAPPASLQPPASAQRQEFL
                     QAADEVMAEMSKLVSLPILSPLKKSMRSREEIRAYLLQKMKEDKDADKRYADQKTMEK
                     FGLLPKDYPLDQVLVKVLTEQIAGLYDPDSQEFFIADWTSPADQRSVMSHELTHALQD
                     QHFHIDKWTDAAKPNDDAELARDAVLEGSAMAAMLDYELRGKGTIRDLGDFDPALFMG
                     DVDSSPELSKAPKVLQDELLFPYLAGITFTQHILKAGNGWADFYKVFENPPASTQQIM
                     HPDLYLQGVMPAKVELPATKGLISVDWKKLDENGMGEFGIQEILKQFLPKERSVATAA
                     SWGGDRYAIFENQKNKRTMLEFRVHLANDADAARFFGAYSEVLDAKYSSRSNLMRRPN
                     FFSFDTPEDGVFLRCMNSDCFVLEGGTRTMFDHMTMEMGWPAGPVVPMNPGDPRLKET
                     AFPIYPALIADAGQPMQPMAQ"
     gene            complement(2514..3170)
                     /locus_tag="VG272_07380"
     CDS             complement(2514..3170)
                     /locus_tag="VG272_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13541.1"
                     /translation="MFLGHFGVAFAAKKASPKTSLGALVFAAQFADLLWPLLLLAGIE
                     KVTIVPGLLPASPFDFTSYPISHSLVAQLGWGALLSLIYFALKRNGRSALLLGALVPT
                     HWFLDFIAHRPDMPIYPGGAKFGLGMWQSVPWTISVEYVLFAAGIALYLNATRAKDGK
                     GKWAFWSLVGLLGVLYTASLFGPPPPSVRALALSALAIWLTVPWAAWADRHRQSEPGR
                     "
     gene            complement(3198..4598)
                     /locus_tag="VG272_07385"
     CDS             complement(3198..4598)
                     /locus_tag="VG272_07385"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HWF13542.1"
                     /translation="MKKDDKFARIAALRPARRSEHPTPLVLEPVEGEDRLAKLLGARI
                     NRNHYGEHLSLLQWYSTPEICTPDARSLSLLLPQVSATQDFLSAAIDPDKWLFLDTET
                     TGLAGGTGTYAFMVGIAWWDAGGLQVEQFFLRDLDEEHSLLLELSERMEQRPVLVTFN
                     GKSFDWPLLETRYRMTRAVPAFSPFLHLDLLHPARQLWRLQLGSVRLKDLERHVLGSD
                     SRGLDWSRLDDIDSSLIPQMYFDYLRGGSAEPLVGIFRHNQMDLRGLAALAGKVLGLL
                     DSGNGIENAARAGTHHPIEVLGLSRIMRKRGHSARARELYETALRFALPRPVERLAQR
                     ELAQIAKRELDYTRAISLWGKLRENPAPRKRSEFPPYAQDAQRALEAAIEAAEQLAIH
                     YEHRAKQPQRALDLIHVAMSELRVARREGTIPKDRAEKIESRLSRRLIRLERRCKNES
                     LLDCTVPRNRALDTIA"
     gene            complement(4822..7221)
                     /locus_tag="VG272_07390"
     CDS             complement(4822..7221)
                     /locus_tag="VG272_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWF13543.1"
                     /translation="MAQSLAVRPLKDAVTLARVREIVEKFAERDRNGEMITAVHHIPA
                     RDAKYAPMPEWVRPELAAAYSEKGVKDLYSHQAMAAASVKEGRNVVVITPTASGKTLC
                     YNLPVLNAVLENPDTRALYLFPTKALAQDQLAELHDLSARLDNRFGVFTYDGDTPADA
                     RKAIRERGHIVLSNPDMLHTGILPHHTRWQRLFENLQYVVLDELHTYRGVFGSHLTNV
                     LRRLRRIARFYGSNPQFICSSATIANPGELASRLAETDFEVIEENGAPAGEKYFVFYN
                     PPVVNRFLGIRRSYINESSRVAQEFLKRNLQTIVFANSRLYTEVLLTYLQQANPVQPG
                     QPPAIRGYRGGYLPGERREIEHGLREGRIRGVVSTNALELGIDVGSLDATIMAGYAGT
                     IASTWQRAGRAGRRSGSSCAVLVASSAPLDQFIVQHPDYFFGRSPEHAFVQPDNLEIL
                     INHLKCAAFELPIATNETFGSADVPDLCNRLAEAGFLHRTGEHWHWTQEAYPADTVSL
                     RSVTSDNFVIVNITDAAEVIGEVDFSSALTTVHPKAIYLHQGQQFHVEALNFEERKAY
                     VKRVDVEYYTDAIRYTQVRVLECAASNRVETGGADAHATRSHGDVLVRSQVIGFKKIK
                     FFTNENVGDGKLELPENEMHTTAFWLTLERPLIESLPFSVSDRQSGMFGLLHALESIA
                     TLLLMCDARDLGTSIGERPPSPGSDIEWEEAESADAIAALAEKEYFEPNLYLYDAYPG
                     GIGFSEPLYRAHAILLAKTRELIAACPCEKGCPSCVGPVGERGERSKEAALAILTRLG
                     A"
BASE COUNT         1729 a         1980 c         2076 g         1699 t
ORIGIN      
        1 caaacgtcag gacattgcct ggaacctggt agagaaatat atttctcgca aactccaaaa
       61 ccgtacagaa atgctgaact atttcacgca aatattgctt acaagaataa aatagaagtt
      121 ggagagaaga ggtccacaac aggatgcccc ctcagtgatt ctgacggtcg cgaagagtat
      181 aattgtaata gagtcgaggt ctaaatgatc gctatttttt cttatagtaa gaagaaaagt
      241 tttattcccc cgctcgtaat acccatcctc gcacttttcg caagttttgt catgcttagt
      301 gacacatttt atttcatcgg catccaggca cagaccaatg ggaatatggc agcggaattc
      361 aagaggactt cggaagaact ctcttcggcc aggttgggcg gcgggagcga aaacaaggtt
      421 caattggaga aggcccttgc ctatctggat tccgcagcca ttttgacgtt aaattccgcg
      481 tcaagtccgg acctcgaagc tgcgaaccgt cggctggcga cgctcgtgtc ccagacctca
      541 ccagtgggag agaattatcg tctgctgaaa atcggcggtt cgcctgccgt atatgcaatg
      601 gcgatcaatt ttggcttggg tggtccggct gccgtgcgga tttatacagg aggaacaggt
      661 cattactcgt tagtggcgca aattgatcat tatgcccaaa aggattttct ggatagcgac
      721 attgaactgg tttctgtttc tcccgccgac atggtgtttg taacggtttc ggggcgaacg
      781 gatgatttat cgactggggc atttacggcc tggcaattca acgggcacgc agtgaccgca
      841 ttatggaatt ctgatttgtt acagcagtcc agctatgagg ttgaggggaa tgggtttcgc
      901 attgcgtatt gctcacaagt ggatgatgac cgtccgtcac aatgcctgaa aatgtcgcgc
      961 gatctctttc gccttgacgc ggggcaatgg aaacgagtcg aaacggctga attgtcctcg
     1021 ccgaaaccta catcgaaata agtaatcttg ccttcgtggc gcttgagcgg aatcgaactt
     1081 ggactttccg cagcatccgt ttatctgcat tccacatcca aatgttaagc ttgatgaggg
     1141 tctcggacgg gaagggatgg accgcgtgaa acgatttctt gtctgcatgg ttactatgct
     1201 ctttctggct gccggggccg gggcgcaaaa cgccgcaccg ccggcgtcgt tgcagcctcc
     1261 cgcatccgct cagaggcagg aatttttaca ggcggctgat gaagtgatgg cggaaatgag
     1321 caaacttgtt tctctgccga ttctttctcc cttgaaaaaa agcatgcgtt cgcgtgagga
     1381 aattcgcgcg tatcttttgc agaaaatgaa ggaagacaaa gacgctgaca agcgatatgc
     1441 cgatcagaag accatggaaa aatttggcct tctgcccaag gactatccgc tcgatcaggt
     1501 cttggtcaaa gttttaactg aacagattgc cggcctgtac gatcccgata gccaggaatt
     1561 cttcatcgcg gactggactt cacctgctga ccagcgttcg gtaatgtcgc atgaactgac
     1621 gcacgccttg caggatcagc attttcacat cgataagtgg acagacgcag ccaagccaaa
     1681 tgatgacgcg gagcttgcac gcgacgctgt gctcgaaggt tccgcaatgg ccgcgatgtt
     1741 ggactatgaa ctgaggggga aaggaaccat tcgggaccta ggcgatttcg atccagcctt
     1801 gttcatgggc gatgtggata gcagtccgga actttccaaa gcaccaaaag ttttgcagga
     1861 tgaattattg tttccgtatc tggcaggaat cacgtttacg caacatattc taaaggccgg
     1921 aaatggatgg gccgatttct acaaagtgtt cgagaaccct ccggcttcca ctcagcagat
     1981 catgcatccg gatttgtacc tgcagggagt tatgcccgcc aaggttgagc tgcctgcgac
     2041 caaaggattg atttccgttg actggaaaaa gctggatgaa aacggcatgg gcgaatttgg
     2101 cattcaggaa atcctgaaac aatttttgcc caaagagcgc tccgttgcca ctgccgcttc
     2161 ctggggtgga gatcgctacg cgatcttcga gaaccagaaa aacaagagga ccatgctgga
     2221 gttccgcgtt cacctggcga atgatgccga tgcggcacgt tttttcgggg cgtatagcga
     2281 agttctggat gcgaagtatt ccagccgaag caacttgatg cggcgtccta atttcttttc
     2341 cttcgatacg ccggaagatg gcgtcttcct tcgctgcatg aattcggatt gtttcgtttt
     2401 ggagggcggc acgcgaacga tgttcgacca catgaccatg gaaatgggtt ggccagcggg
     2461 gcccgtagtc cccatgaatc caggcgatcc gcgtttgaaa gaaacggcgt ttcctatcta
     2521 cccggctctg attgccgatg ccggtcagcc catgcagccc atggcacagt aagccagatg
     2581 gccaatgcgc tcaacgcaag cgctcgcacg ctgggaggcg gagggccgaa caaggacgct
     2641 gtgtagagaa cgccaagcaa accaacgagc gaccaaaaag cccactttcc tttaccgtct
     2701 ttggctcgag tcgcgttgag ataaagtgca atgcccgccg caaacaacac atactccacg
     2761 gaaatcgtcc aaggcacgga ttgccacatc cccagaccga attttgcgcc gccgggatag
     2821 atcggcatgt cgggccggtg cgcaataaaa tccagaaacc aatgcgtagg gaccaaagct
     2881 cccaggagca acgcgctcct gccatttcgc tttaaagcaa aataaatgag gctcagcaac
     2941 gctccccaac cgagttgcgc gaccaggctg tgcgaaattg gatatgacgt gaaatcgaat
     3001 ggcgaggccg gcagaaggcc cggcacgatg gttacttttt cgatgccggc gaggagcagc
     3061 aacggccaca gcaggtccgc gaattgcgcg gcaaacacca gcgcacccaa agaagtttta
     3121 ggcgatgctt ttttcgcggc aaacgccact ccaaaatggc cgaggaacaa attgtcctcc
     3181 tttatgtact cgagtagcta ggcgattgta tcaagggcgc ggttccgggg tacggtgcaa
     3241 tccaataatg attcgttttt gcagcggcgc tccaatcgaa taaggcggcg ggaaaggcgc
     3301 gattcaattt tttcagcccg atctttagga attgttcctt cgcggcgggc aacgcgcagt
     3361 tcgctcatgg ccacgtgtat taaatccagc gcgcgctgcg gttgtttggc tcgatgctcg
     3421 taatgaatcg ccaattgttc ggcggcttcg atagccgcct ccagtgcccg ctgcgcgtct
     3481 tgtgcgtaag gcggaaattc agaacgtttt ctaggggcgg gattttctcg cagttttccc
     3541 caaagggaaa tagcacgcgt gtagtcgagt tcgcgtttcg cgatttgcgc aagttcgcgt
     3601 tgtgcgagcc gctcgacagg acgcggcagg gcgaagcgca acgcggtttc atacaactcg
     3661 cgagcgcgcg cggaatggcc acgcttgcgc atgattcgcg agaggcccag cacctcaatg
     3721 ggatggtgcg tgccggcgcg tgcggcattt tcaattccat ttccgctatc gagcaggcct
     3781 aggacttttc ctgccagcgc agcgagtccc cgcaagtcca tctgattgtg acgaaaaatt
     3841 cccaccagcg gctctgcgga tcctccgcgg agataatcga aatacatttg cgggatgagc
     3901 gaggaatcga tgtcatcgag tcgagaccaa tcaaggccgc gactgtcgct gcccaagaca
     3961 tgacgctcaa gatccttgag gcgcacggag ccaagctgaa gccgccacaa ttggcgcgcc
     4021 gggtgcaaca agtcgagatg cagaaatggg gagaaagccg gaacggctcg cgtcatccga
     4081 tagcgcgttt cgagcagcgg ccagtcaaaa ctttttccgt taaatgtcac gaggacggga
     4141 cgctgctcca tgcgctcgga aagttccagc agaagcgaat gttcctcatc gagatcgcgc
     4201 aggaaaaact gctcgacttg cagcccgccc gcatcccacc acgcgatgcc caccatgaat
     4261 gcgtaggtac cggtgcctcc cgcgaggcct gtggtttccg tgtcgaggaa cagccacttg
     4321 tcaggatcaa tggcggcact taaaaaatct tgcgtcgccg aaacttgagg aagaagcagg
     4381 gaaagagagc gcgcatcggg cgtgcaaatc tctggagtgg aataccactg caacaatgat
     4441 aaatgttcgc cgtaatgatt gcgattgatg cgcgcgccaa gaagttttgc gagtcggtct
     4501 tctccttcga ctggttccaa aaccaatgga gttgggtgtt cgctcctgcg tgctgggcgc
     4561 aacgccgcaa tccgcgcgaa tttgtcatct ttcttcatac ttgccacgga tccacagaga
     4621 cacggagaaa tgctgggaac cacggattca cacggatcaa cacggataat gaaaaaacaa
     4681 aatgcaaata agtgaaaaat agataacgac gaaagaaatt cagatctctc ccattttttg
     4741 aattgtcttt catccgcgtc aatctgtgtc catccatggt ttcatttctc tcagtgtctc
     4801 ggtgtctcgg tgactgaatt ttcatgcgcc aaggcgagta aggattgcca gggcagcttc
     4861 cttggagcgc tctcctcgct cacctacagg gccaacacat gacgggcatc ccttttcgca
     4921 agggcatgct gcgatcagtt cgcgtgtctt tgccagtagg atagcgtgtg cgcggtacag
     4981 cggctcggaa aatccgattc cgccgggata agcgtcgtac aaatataaat tgggctcgaa
     5041 gtattctttt tcggcgagag cggcaatcgc atcggcgctc tccgcttctt cccactcgat
     5101 atccgagccg ggcgagggcg ggcgttcacc gatcgaagtc ccgaggtcgc gcgcgtcgca
     5161 catcaatagc aatgtcgcga tggattccaa tgcgtgcagc aatccgaaca tgccgctctg
     5221 gcggtcgctc acggaaaagg gaagcgattc gatcagcggc cgctccagag tcagccagaa
     5281 agccgtggtg tgcatttcat tttcgggcag ttcgagtttt ccatcgccta cattttcatt
     5341 ggtgaagaat ttaattttct tgaagccgat cacctgtgaa cgaaccagca cgtcgccgtg
     5401 cgagcgagtg gcgtgcgcgt cggcgccacc cgtttcgacg cgattgctgg ccgcacattc
     5461 caagacgcgc acctgtgtgt agcgaatggc gtcagtgtaa tattccacgt caacgcgctt
     5521 cacgtatgct tttcgctcct caaagttcag cgcttccaca tggaattgct ggccctgatg
     5581 cagataaatt gccttggggt ggactgtggt gagtgcgctc gaaaaatcca cttcgccaat
     5641 cacttctgcc gcgtccgtaa tattcacgat cacaaaattg tccgaagtga ctgagcgcag
     5701 gctcacggtg tccgcgggat acgcttcctg cgtccagtgc cagtgttcgc cggtgcgatg
     5761 caaaaaacct gcctcggcga ggcgattgca caggtcagga acatccgcgc tgccaaatgt
     5821 ttcgttcgtc gcgatgggca attcaaacgc ggcgcatttc aggtgattga tcaggatttc
     5881 caaattgtcc ggctgcacaa aagcgtgctc aggcgaccgt ccgaaaaaat agtccggatg
     5941 ctgcacgatg aattggtcaa gtggggcgga ggaagccacg agcacggcgc aagaactgcc
     6001 gctgcgtcgt cccgcgcggc cagcgcgctg ccatgtggag gcaatggttc cggcgtatcc
     6061 ggccatgatc gtcgcgtcga gcgaaccgac atcgattccc agttccagcg cgttggttga
     6121 aaccacgccg cgaatgcggc cttcacgcag gccgtgctcg atttcgcggc gctcgccggg
     6181 aagatagcct ccacgatagc cgcgaatcgc gggcggctgg cctggctgca caggattggc
     6241 ttgctgcaaa taggtgagca gcacttcggt gtacaggcgc gaatttgcga aaacgatggt
     6301 ctgcaaattt cgtttcaaaa attcctgtgc gacgcgcgac gattcgttga tatagctgcg
     6361 ccgaattccc aggaaacggt tcacgaccgg cggattataa aagacgaaat atttttctcc
     6421 ggcgggcgcg ccgttttctt cgatcacctc gaaatcagtt tccgcgaggc gagaggcgag
     6481 ttcgccggga ttcgcgatcg tcgccgatga gcaaatgaat tgcggattgg agccgtagaa
     6541 gcgcgcaatg cgccgcaacc ggcgcagcac attcgtcaga tggcttccga atacgccgcg
     6601 atacgtgtgc agttcatcca gcaccacata ctgcaaattt tcaaacaggc gctgccaccg
     6661 cgtgtgatgg ggaagaattc ctgtgtgcag catgtccgga ttcgagagca cgatgtgccc
     6721 gcgctcgcga atcgctttgc gcgcatcggc gggagtgtcg ccgtcataag tgaaaactcc
     6781 gaagcgattg tcgaggcgcg cggataaatc gtgcaattcg gcaagctgat cttgcgcaag
     6841 ggctttggtg gggaagaggt agagcgcgcg ggtatccgga ttttcgagca ccgcattcag
     6901 caccggtaag ttgtagcaaa gcgtttttcc ggatgccgtc ggcgtaatca ccactacgtt
     6961 gcgcccttct ttcacggacg ccgcagccat ggcttgatgg ctgtaaagat cctttacccc
     7021 tttttccgaa tatgcggcgg cgagctcggg gcgcacccat tccggcatgg gcgcatactt
     7081 cgcatcgcgc gcgggaatgt ggtgtacggc ggtgatcatc tcaccattcc ggtcgcgctc
     7141 cgcgaatttc tcgacgatct cgcggacgcg cgcaagcgtt accgcgtctt tcaagggccg
     7201 tacggccaaa gattgagcca caactcgtct cctgtatgct tcgcctttta ttcgctacgc
     7261 taacatgccg ccgcggacag tgcaacggtc tttctctcaa taatttgcaa tggcgttcga
     7321 atttttaaac tttttttcgg cctgactatc cgtcaaaggt ctgagttaat ttcccgcgcg
     7381 cgttttccct gtcacaacgg gcgtatcccg gccctgtcct tcttcagtcc cattgaccag
     7441 tcgaaccatt tattccgcgt gacctagtct gcaaccaggg cgga
//
LOCUS       DASZMB010000163        10321 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_457351, whole genome shotgun sequence.
ACCESSION   DASZMB010000163 DASZMB010000000
VERSION     DASZMB010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 10321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10321
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457351"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /gene="fabV"
                     /locus_tag="VG272_07395"
     CDS             complement(<1..1059)
                     /gene="fabV"
                     /locus_tag="VG272_07395"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF010177.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabV"
                     /protein_id="HWF13544.1"
                     /translation="MRFYATLRYYLLLMPLQIIKRRSRGFICVNAHPEGCRRNVERWV
                     AGVKGEMPAGQNAPKNVLVIGASTGYGLASRIAAAWGYGAKTLGVFFERPPDGDKTAT
                     AGHYNTVAFHSLAKSDDLFAASVNGDAFSDEIKRATMEVIRKQMAPIDMVIYSLASPK
                     RTDPRTGKTHSSVLKPVGQSFTNRTIELDSEKVVDITLQSANDEEVADTVAVMGGDDW
                     RLWIEMLLEEKLLAPGARTLAYSYIGPEITWPIYRDGTIGQAKKDLERAAKDLDSTLA
                     EQLGGHAWVSVNKAVVTQASAAIPVVPLYLSLLSSVMKKKNLEEGPLEQMRRMFTDFL
                     CTGSAPLLDDLRRLRLDDR"
     gene            1196..1852
                     /locus_tag="VG272_07400"
     CDS             1196..1852
                     /locus_tag="VG272_07400"
                     /inference="COORDINATES: protein motif:HMM:NF016240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HWF13545.1"
                     /translation="MDILLNDIETRVLGALVEKELTTPEYYPLSLNALVNACNQKSNR
                     DPAMNLDEDSVREAIRTLEKKGLAGPADNMVSRVTKYEHRLQEAYNFTRHETAILAEL
                     LLRGPQTPSELRTRADRMHKFDDLGIAQSTLQRLMKREPPIVKVLPRQPGTKEARYAH
                     LLSGDVESPPQESAAGVAATSSAAGGDRIARLENQIEVVQSEIAELKQQFAAFRKQFE
                     "
     gene            complement(2236..3339)
                     /locus_tag="VG272_07405"
     CDS             complement(2236..3339)
                     /locus_tag="VG272_07405"
                     /inference="COORDINATES: protein motif:HMM:NF005071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWF13546.1"
                     /translation="MKMRFAIWKVTLLVAVLSMVATCASAQQNTTVAGLQPKEGDYVT
                     HDFRFSDGQILPDVKLHYMTLGTPQHDASGKITNAVLILHGTNRAGRVWLTPTFAGVL
                     FGPGQLLDASKYYLILPDQLGAGLGKSTKPSDGLRGKFPHYDYSDMIKASQILLAEGL
                     RVNHLHLLVGASMGCMEGFMWAEASPDDMDAMLLLSCLPVEVASRNRMVRKIMVDDIR
                     LDPGWNNGNYTEQPYGLRAALGHLLVVGSAPTRWQKEYPTAKAADKYVNDYIEQNMKT
                     TDANDFMYQYDASRNFDASKGLDKITSKVLLINPQEDFWNPAELGIAEEEIKKVKNGK
                     FVLLSFSESYGHYTFFQAAAWQKYFAAFMKELK"
     gene            complement(3461..5926)
                     /locus_tag="VG272_07410"
     CDS             complement(3461..5926)
                     /locus_tag="VG272_07410"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HWF13547.1"
                     /translation="MPEAVEGKNVETESVFEGYRRWGYLAADLDPLGFLAPIVHPELI
                     VEGDAAAQARKFYCRTIGVEFQHIDDTERRRWIAERVESEPAQPDSEFILERLIRADF
                     FEQVLQARYLGSKRFSLEGVTAVIPLLDRVLSAAAESGAEQVMLGMSHRGRLNVMVHI
                     AGQKAADIFAGFEDVDPRSVLGSGDVKYHVGATGEYRTHEGRRLSVHLASNPSHLEAV
                     DPVVVGRARAKQTRAGLPSGIKKVLPIVLHGDAAFAGQGVWAETLNLADVDAYSVGGT
                     IHVIVNNLMGFTTQPLELHSSRFASSIAKRQAVPIFHVNGEDPDAVVRVGQMALDYRY
                     KFGGDVVIDVIGYRRHGHSEVDDPTITQPLLYAEIKDHPQLWQIYAKQIAVDAAATVT
                     AIRAELENAHKAASEMQKKPRLRKLPAYWSPYKRGRYDSAYEVDTGVAPAELAKITDL
                     LTSYPPDFAIHPKVKKLLDQRAEMGHGRRAVDYGMAEALAFGTLLLSGTPVRFTGQDT
                     RRGTFNQRHSALVDTRNEAQYMALQHLSPDQAKCEIDNSTLSEAAVLGFEYGFSRDFP
                     EALVLWEAQFGDFANSAQIIIDQFISAAEDKWDLPSGVGLLLPHGYEGQGPEHSSARI
                     ERYLQLAAEDNIQVCQPSSAAQYFQLLRRQALRKWRKPMVVFTPKSMLRHPDASSPIE
                     DFQRPRFEWVVPDREVNSAERVLICTGKIGHELRGERKRRGDTNTAILFLDQLYPFPK
                     NELRAEMARHPNAREIVWVQEEPANMGALSYVLPRIRTYFPDVPVRSVKRSASASPAT
                     GSAKAHELEQKTVLTFAFTTGPN"
     gene            complement(6173..6463)
                     /locus_tag="VG272_07415"
     CDS             complement(6173..6463)
                     /locus_tag="VG272_07415"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HWF13548.1"
                     /translation="MAATKLAEAEVAVELRKLDGWSVEKGNLHRVFEFKDFSEAFGFM
                     SRVALAAEKMDHHPDWSNVWNKVTVDLSTHSAGGLTKNDFDLAGKIQQIYGS"
     gene            6929..7942
                     /locus_tag="VG272_07420"
     CDS             6929..7942
                     /locus_tag="VG272_07420"
                     /inference="COORDINATES: protein motif:HMM:NF040257.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13549.1"
                     /translation="MKKKIRAVQYGVGPIGAAIVRLMREKEAIEIIGAIDSDPAKAGR
                     DLGDVVGGTDAPWGVSISSDAETMLAKSPDVVIHSTSSYLPDVMDQLLACLAAECCVV
                     STCEELSYPFRKHPELSAKLDAEAKTWGVALIGTGINPGFVMDKLLITLSAASQTTGS
                     VRAVRIVDASKRRLPLQKKVGAGMTELEFRAQVAAGIIKHHGLPESVAMVSDALGLGV
                     DDISETIEPVIAREAVTTPFLEVAPGQVAGVHQIARGYAGSKEKIFMELQMFVGATNP
                     GDTVEITGNPNLTLTIPGGTHGDIATASVVVNCIPAILDATAGLRTSRDLPMCFLPPN
                     AKP"
     gene            complement(7951..8820)
                     /locus_tag="VG272_07425"
     CDS             complement(7951..8820)
                     /locus_tag="VG272_07425"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWF13550.1"
                     /translation="MKPSVGLIGLGLMGRPMGANLLKAGHDLTVWNRTASRADELVSQ
                     GAKRAGSPRELAAASEVVITIVSDPPALESVLWGDSGVFAGLRAGSVIIESSTVSPDL
                     EKRAAATAALHGAEFLEAPVTGGTWGAEKGELVFMVGGEEKTLKRVEPVLGVMGKKWF
                     HLGPIGAGQTVKVAMNLLLDLEVEAFAEALALVTRAGVPGQSLLEVMQSSMGRSPVID
                     LKGANMLKGEYKPSFPLRLMHKDLGLALDLANQLGVPLPAAAAAREVLSAVKGAVTED
                     VDFSALATFWKKK"
     gene            complement(8923..9228)
                     /locus_tag="VG272_07430"
     CDS             complement(8923..9228)
                     /locus_tag="VG272_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13551.1"
                     /translation="MARYSDLLGRRIVVTYRAGDIILPATGNLAADSGKSIFLEESYK
                     QRGNLKTFRWEIPYMCIVELHECPEVPKPSASLIEPRETSENATRVPGTLELKNRPI"
     gene            complement(9355..9726)
                     /locus_tag="VG272_07435"
     CDS             complement(9355..9726)
                     /locus_tag="VG272_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13552.1"
                     /translation="MAVRICPHCKTEISASISAARSNDMECPKCEARLEVVSGSRTIS
                     SLCGLAAGTIAWRLSRGSTSDLGGVLPTLYAFLAFGIVSPLVLMFTANLRNAPALPVP
                     VPAQAGSHDAHGAAGHGSDHH"
BASE COUNT         2383 a         3003 c         2622 g         2313 t
ORIGIN      
        1 gcgatcatcg aggcgcaacc gccgcaaatc gtccagcaaa ggcgcgcttc ccgtgcaaag
       61 gaaatccgta aacatgcgcc gcatttgttc gagcggccct tcttccagat ttttcttttt
      121 catcaccgag gaaagcaaac tcaaatacag cggcacaaca ggaatcgccg ccgatgcctg
      181 ggtgaccacg gccttgttca cggaaaccca tgcgtgcccg cccaattgtt ccgcgagtgt
      241 gctatccaaa tccttagcag cgcgctccaa atctttcttt gcttgcccga tggtgccatc
      301 gcgatagatc ggccacgtaa tttccggacc gatgtacgaa taggccagcg tgcgcgcgcc
      361 cggcgccagc aatttttcct cgagcagcat ttcaatccac agccgccaat catctcctcc
      421 cattacggca accgtgtccg caacttcctc gtcatttgcg gactgtagcg tgatgtccac
      481 cactttttcc gaatccagct cgatcgtgcg gttcgtaaat gattgcccca ctggcttcag
      541 aaccgagctg tgcgttttgc ctgtccgcgg atcggtgcgt ttcggcgacg cgagcgagta
      601 aataaccata tcgatggggg ccatttgctt gcgaattact tccatcgtgg cgcgtttgat
      661 ttcgtccgaa aacgcgtctc cgttgacgct cgcagcaaaa aggtcgtcgc tcttcgccag
      721 tgaatggaat gcaaccgtgt tgtagtgacc cgcggtcgcg gttttgtcgc catctggcgg
      781 tcgctcaaaa aatacgccca gcgttttcgc tccatagccc caggccgccg cgatgcgcga
      841 agcgaggccg tagccggtgg acgcgccgat aaccagcaca ttcttcggag cattttgtcc
      901 ggcaggcatt tcccctttga ctccggcaac ccaacgttcc acatttcgac ggcacccctc
      961 ggggtgagca ttcacacaga tgaaaccgcg cgagcggcgt ttgataattt gcagtggcat
     1021 aagtaaaaga taataccgta gcgtggcata aaaccgcatt tgctttcgta gcgcacggcc
     1081 ggttttcagg gcgggcagcc ttggcgcgca tgcagtgcaa aacaatatgc catcggaaat
     1141 cgcctcgcgt tgagcagttg ggaaccccag tgcaatacgt tatgattttc tcctcatgga
     1201 cattctgcta aacgacattg aaacgcgggt gctcggcgcg ctcgttgaaa aagagctgac
     1261 tacgccggaa tattatccgc tctcgctcaa tgcgctggtc aacgcgtgca atcaaaaatc
     1321 aaatcgcgat ccggcgatga atctggacga agattccgtg cgcgaagcga ttcgtactct
     1381 ggaaaagaaa gggctggcgg gaccagctga caacatggtc agccgcgtga ccaaatatga
     1441 gcatcgcctg caggaagctt acaacttcac gcggcacgaa acagccatcc tcgctgaact
     1501 gctgctgcga ggcccgcaaa cgcccagcga attgcgaacc cgcgccgatc gcatgcacaa
     1561 gttcgacgat ttgggcatcg cgcagtccac cttgcagcgc ctgatgaagc gggagccacc
     1621 gattgtcaaa gtgcttccga ggcagcccgg cacaaaagaa gcgcgttacg cacacctgct
     1681 cagcggtgat gtggaatcgc cgccgcagga atcagcagcg ggagtggcgg caacgagcag
     1741 cgccgcgggt ggcgatcgta tcgcgcgact ggaaaatcaa attgaagtgg tacagtcaga
     1801 aatcgcggag ttgaagcagc aattcgccgc attccgcaaa cagttcgaat gatttcggta
     1861 ggacaggctt tagagcctgt gtgagaactg tagaatcttt gtgggtcgtg gctttagcca
     1921 cgacataagt cctgcaaaat gaatgcggct ttacagtttg cggaaaaagt agtttattct
     1981 gtcattccga gcgaagcgag gaatctctct tcgacttaag tataagaaaa caaagaagga
     2041 gggattccgc gcttcgctcg gaatgacaaa atgttgagtt tttccgcagc ctgtttagcc
     2101 gctgaagctt caattttaca gttctcacgc agactcttca gcctgtcttc tttttcatcc
     2161 agcactacat tctttttttg acggcaggct ctttgcgccc tcaaacaaat caaaccagac
     2221 agactgaagc ctgtcctact ttaattcctt cataaacgcg gcaaaatatt tctgccaggc
     2281 agccgcctga aaaaatgtgt agtgcccata cgattcggaa aacgacagca aaacaaattt
     2341 cccgtttttc actttcttaa tttcttcttc cgcgattcct agctccgccg gattccagaa
     2401 atcttcctgc ggattaatca gcaaaacttt ggaggtaatt ttgtcgaggc ctttcgatgc
     2461 atcgaaattc cgcgacgcat catactggta catgaaatcg ttcgcatcgg ttgtcttcat
     2521 gttctgctcg atgtaatcgt tcacatattt gtcggccgcc ttggcggtcg gatattcctt
     2581 ctgccagcgc gttggcgcgc ttcccaccac aagcaggtga cccaaggcgg cgcgcaatcc
     2641 gtatggttgc tccgtatagt taccgttgtt ccatccggga tcgaggcgaa tgtcgtccac
     2701 cattattttt cggaccatgc gattgcggct ggcgacttcc accggcaagc aagacagcag
     2761 cagcatcgcg tccatatcgt cagggctcgc ttccgcccac ataaaacctt ccatgcagcc
     2821 catcgaagca ccgacgagca aatgcagatg gttcactcgc aaaccttccg cgagtaaaat
     2881 ttgcgaggcc tttatcatgt ccgaataatc gtagtgcgga aattttccgc gcaatccatc
     2941 gcttggtttt gtcgatttgc cgagacctgc gccaagttga tcgggaagaa tcagataata
     3001 tttcgacgcg tcaagcagct ggccggggcc aaagagaact ccggcaaagg ttggagtaag
     3061 ccaaacgcgg cccgcgcgat ttgttccgtg cagaattaaa acagcgttgg tgattttacc
     3121 gctggcatca tgctgaggtg tgccgagcgt catatagtgc agcttcacat ccggaagtat
     3181 ctgcccatcc gaaaatctga aatcatgcgt aacgtaatcg ccttctttgg gctgtaaacc
     3241 ggcaaccgtt gtattttgct gagcggaggc gcatgtggcg accatagaca aaacggccac
     3301 caacaatgtg actttccaga ttgcaaatcg cattttcatt ttctcctaaa aaattacaca
     3361 ccttcgaaac tattcgcgca tgaatatagc accggcgtct caccggcacg gtttagtgca
     3421 agcaaggact aaaaccgcac aggctgaagc ctatgccacg tcagttcgga cccgtagtaa
     3481 acgcaaacgt cagcacagtt ttctgttcta gctcgtgcgc cttcgctgac ccggtcgcgg
     3541 ggcttgcgct ggctgaacgt ttcaccgagc gcaccggaac atccggaaaa taagtccgaa
     3601 tgcgcggcaa tacatacgac agcgcgccca tattcgccgg ctcttcctgc acccatacaa
     3661 tttctcgcgc attcggatgg cgtgccattt ccgcgcgcaa ttcatttttc ggaaatggat
     3721 aaagctggtc cagaaaaaga attgccgtgt tcgtatcacc gcggcgtttt cgttccccgc
     3781 gcaattcatg gccgattttc ccggtgcaaa tcaatacgcg ttcagcggaa ttaacttcgc
     3841 ggtccggcac gacccattca aatcgaggcc gctgaaaatc ctcaatcggc gaagacgcat
     3901 caggatgccg cagcatgctt ttcggcgtaa acacaaccat tggcttgcgc catttccgca
     3961 gcgcctgccg ccgcaacaat tgaaaatact gcgccgcgct cgacggctgg caaacttgaa
     4021 tattgtcctc ggccgctaat tgaagatacc tctcaatcct tgcgctggaa tgttccggac
     4081 cttgcccttc ataaccatgc ggcaagagaa gtcccacgcc cgaaggcaag tcccacttgt
     4141 cctccgccgc gctgatgaac tggtcaataa taatttgcgc gctgttcgcg aaatcgccga
     4201 actgcgcttc ccacagcacc agggcttcag gaaagtcgcg gctgaatccg tattcaaaac
     4261 cgagaacggc ggcttcggaa agcgttgaat tatcaatttc gcattttgcc tggtcaggag
     4321 ataaatgttg gagtgccata tactgcgcct cgttgcgtgt gtcgacgagc gcggaatgcc
     4381 tttgattgaa tgttccgcgc cgcgtgtctt gtcctgtgaa ccgcacggga gtccccgaca
     4441 gcagcagcgt tccgaacgca agcgcttccg ccatgccgta atccacggcg cgccggccat
     4501 gccccatctc ggcgcgctgg tccagcagct ttttcacttt tggatgaatc gcgaaatccg
     4561 gcggatagct ggtcagcaaa tccgtaattt ttgcgagttc ggcaggcgca actcccgtgt
     4621 ccacttcgta ggcactatcg tagcggccgc gcttgtaagg agaccaatac gcaggcagct
     4681 tgcgcaggcg cggctttttt tgcatttcgc tggcggcctt gtgcgcgttt tccaattcag
     4741 ctcgtatcgc cgtgaccgtc gcggccgcat ccaccgcgat ttgtttcgcg taaatctgcc
     4801 agagctgcgg gtgatcttta atttccgcgt atagaagcgg ctgcgtaatc gtcggatcgt
     4861 ccacttcact gtgaccgtgc cgtcgatacc caatgacgtc aatcaccacg tctccgccaa
     4921 acttatatcg ataatcgagc gccatctgcc ccacgcgaac gaccgcgtcc ggatcttcac
     4981 cattcacatg aaaaatgggc acagcctggc gcttggcgat cgaagaagca aagcgcgagg
     5041 aatgcaattc cagaggctgc gtggtgaatc ccatcaaatt atttacgatc acgtggatcg
     5101 tgccacctac gctatacgca tcgacgtcgg caagattcag cgtctccgcc caaactccct
     5161 gcccggcaaa agccgcgtct ccgtgcaaaa caattggcaa tacttttttt atcccgctcg
     5221 gcaggcccgc gcgtgtttgc ttggcgcgtg cacggcctac gaccacggga tcgacggcct
     5281 caaggtgact gggattggat gcgagatgaa cgctgagtct gcgcccttcg tgcgtgcgat
     5341 attctcccgt tgcgccgacg tgatacttca cgtcgccgct gcccaacacg ctgcgcgggt
     5401 ctacatcctc gaagccagcg aaaatatccg cggccttttg tccagcgatg tgcaccatta
     5461 cgttcaggcg cccgcggtgg ctcattccga gcatcacttg ctccgcgcct gattccgccg
     5521 cggcggaaag cacgcgatcg agcaacggaa tcacggccgt tacgccttcc agcgagaatc
     5581 ttttgcttcc cagataacgc gcctgaagca cctgctcaaa aaaatccgcg cgaatcagcc
     5641 tttccagaat aaattcactg tcgggctgcg ccggttcact ttccacgcgc tcggcaatcc
     5701 agcgccggcg ttccgtatcg tcaatgtgct ggaattcaac gccaatcgtc ctgcaataaa
     5761 atttacgcgc ctgtgcggcc gcgtcacctt ccacaatcaa ttcaggatga acaatcggag
     5821 caagaaaacc gagtgggtcc agatcagccg cgaggtatcc ccagcgacgg tagccctcaa
     5881 acacactctc cgtctcgaca ttttttcctt cgacagcttc cggcattttt ccagcttccc
     5941 caatcctctt ataaggacat aaacgaacaa tttattctat caaacgcgag gtgcgggaag
     6001 cgttccggaa ataacttcga cgggcttttt tgcaggaaat aatttttagc tgaacaaaat
     6061 gatactgggt cagtttgaaa tttagggtga cttgaacttg tttcaatttt cggcctaaat
     6121 tcccggtctc atttccggtg gtgcgctgcg ggcgaagaga aaattacggc cgctaagacc
     6181 cataaatctg ctgtattttc ccggcaagat cgaaatcatt tttcgtcagc ccgccggcgc
     6241 tgtgcgtgga taaatccacc gtaaccttgt tccagacgtt ggaccagtcg ggatgatgat
     6301 ccattttttc ggcggcaagg gccacgcgtg acatgaatcc gaatgcctct gaaaaatcct
     6361 tgaactcaaa cacgcgatga agatttccct tctccacgct ccagccatct agttttcgca
     6421 gttccaccgc tacttctgcc tctgcaagtt tcgtcgccgc catgaagcct cctgtctcac
     6481 ttgttctcct gccagactct gtctcataaa taatctttgt gggtcgcagc ttcagccgcg
     6541 acattcaaaa gctcaaaatg aatgggctaa agagtctgtg tgagaacaat agagtcattg
     6601 tgggtcgtgg ctttagccac gacataaggt ctgcaaaatg agtgcggctt tagccgctga
     6661 agttccaatt ttgcagttct cacacagact ctttagcccc tggcagtccg tcgaattgga
     6721 acgcgcccat ttatgagact acttctagta tccgcccgcc gacgcccgca aaacaagcta
     6781 cctgcttttt tgcctaaaaa tagggactga atcccgttga acgcgccgcg cgtttgcttt
     6841 atggtaatcg ctccaatttg tagggtaacc agtcgccgcc ccggacgccg acgatcaaaa
     6901 attcccagtt cagcgcaacg gagcagtcat gaaaaagaaa attcgcgcgg tgcagtacgg
     6961 agtcggtccg atcggtgcag ccatcgtgcg cttgatgcgg gagaaagaag caattgaaat
     7021 tattggagcg atcgattcgg atccggccaa ggctggtcga gacttgggcg atgtggttgg
     7081 cggaacagat gccccttggg gcgtttcaat ctcgagtgac gccgaaacca tgctcgcgaa
     7141 atccccggat gtggtcattc attcgacttc atcctatctg cccgacgtga tggatcaatt
     7201 gctggcgtgt ctggcagcgg aatgttgcgt cgtctcaact tgcgaggaac tttcgtatcc
     7261 gtttcgcaag catccggaac tttcagccaa gctcgatgcc gaggcgaaaa cctggggcgt
     7321 cgcgctaatc ggcactggaa ttaatcccgg cttcgtgatg gacaaactgc tgatcacgct
     7381 gagtgccgct tcccagacca caggatccgt gcgcgccgtg cgcatcgtgg acgcgtccaa
     7441 gcgccgcctg ccgctgcaga aaaaagtggg agcgggaatg accgaactgg aattccgcgc
     7501 gcaggtggcc gccggaatca ttaaacatca tggcttgcct gagtccgtgg ccatggtttc
     7561 ggatgcgctc gggcttggcg tggatgatat ttcagaaacg atcgagccgg tgatcgcgcg
     7621 cgaagccgta acgactccgt ttctggaagt cgcgcccggc caggtcgccg gcgtgcacca
     7681 gatcgccaga ggctacgcag gcagcaagga aaaaattttc atggaattgc aaatgtttgt
     7741 cggcgcaacc aatcccggcg acacggtcga gatcaccggc aatcccaacc tgacgcttac
     7801 cattcccggc ggcacgcacg gcgacatcgc caccgcttcc gtggtcgtaa attgcatccc
     7861 ggcaatacta gacgcaaccg caggcctgcg cacatcgcgc gatctcccaa tgtgctttct
     7921 gccgcccaac gcgaaaccgt aaccggaaaa ttattttttc ttccagaatg tagcgagcgc
     7981 cgagaaatcc acatcttcgg tcacggcccc cttcacggcg ctcaacactt cgcgagcggc
     8041 cgcggccgca ggcagaggca ctcccagttg attagcaaga tcgagcgcca agcccaaatc
     8101 cttgtgcata agccgcagag gaaagctggg cttgtattcg cccttcaaca tattcgcgcc
     8161 cttcaaatcg atcacgggcg agcggcccat gctcgattgc atcacctcga gcaggctttg
     8221 tcccggcact cctgcgcgcg taaccagcgc gagcgcctcg gcaaacgcct ccacttccag
     8281 atcgagcaac aaattcatcg cgaccttcac cgtttgcccc gccccgatcg gtcccagatg
     8341 aaaccatttc ttgcccatta cgccaagcac gggttcaacg cgcttcaggg ttttttcttc
     8401 gccgcccacc atgaacacga gctcgccttt ttccgcgccc caggttccgc ccgtcaccgg
     8461 cgcttccaaa aattcagcgc catgcagcgc agccgtcgcg gcggctcgct tctcgagatc
     8521 cgggctgacc gtgctcgatt cgattatcac gcttcccgcg cgcaatcctg caaacacgcc
     8581 gctatctccc cacaataccg actccaacgc cggtggatcg ctgacaattg ttatcaccac
     8641 ttcggatgcc gcagccaact cgcgcggcga accggctcgc ttcgcgccct gtgacacaag
     8701 ttcgtcggcg cgggaagccg tgcggttcca taccgtaagg tcgtggccgg cctttaacaa
     8761 attggcgccc ataggacggc ccatcaatcc caatccaatc aatccaaccg acggcttcat
     8821 gatgaaaatc ctcccgagca aataaatttc tggaaacgtg tgaaagcaaa agtttacagg
     8881 agatcggtcc aaatccggat gaaaatcgac ggaggaaaaa acttagattg ggcgattctt
     8941 gagttcgaga gtcccgggaa cccgtgtagc attttcggaa gtttcccgcg gctcaataag
     9001 ggaggcggag ggcttgggaa cttcggggca ttcatgtagc tccacaatgc acatatatgg
     9061 aatttcccaa cgaaaggttt tgaggttgcc gcgctgctta taactttctt cgaggaagat
     9121 ggacttgcct gagtcggcag ccagattgcc cgttgcgggc aatataatat ctccggcgcg
     9181 gtacgtaacc acgatgcggc ggcctaacag atctgaatac cgtgccatgc tgaaagtatg
     9241 gcgagggcaa ggaagcggga caatagtacg atggtgtcaa atgaattagt acttggctct
     9301 atgcccagca attgccatta gatagccatg ccccaaaagc gaacctagcc acgcctaatg
     9361 atgatcgctg ccgtgtccag cagcaccgtg cgcgtcatgg ctacctgctt gcgcaggaac
     9421 gggaaccgga agcgccgggg cattccgcaa attggcagtg aacatcagca ccagtggcga
     9481 gacgatgcca aaagcaagga acgcgtaaag agttggaaga acgccgccga ggtcactggt
     9541 cgaacccctt gacaggcgcc aggcaatcgt ccccgccgcc aggccgcaca gactggaaat
     9601 ggtgcgcgat ccggaaacca cctccagccg cgcttcgcac ttcgggcatt ccatatcatt
     9661 cgaacgcgcc gcggaaatcg acgctgagat ttccgttttg caatgcggac agatgcgaac
     9721 agccacgtct cacctccacg atggagcacc tacaataatc gaaagcaagc ctggaagaaa
     9781 agacattaaa cggcagtgcc tcaatccgcg tggcaaagcg ccctctacaa aacccgtaac
     9841 gctttgccgt tgtttttgta gcgcacgccc ttcttttcgg gcggacagct tttccgctac
     9901 cgcacttcaa tggcaagcct cgacgaataa tcgatggaat attccgcctt tgtatttgtg
     9961 cgaattgcac tacggacaaa ttggaatttc aggatgcgta tctttcccgt cgctcggagc
    10021 gctatttaca catgcggcat ggccccgcga tcgcgaattt cacttcccga atccatggct
    10081 acaatactgt cgtctccggc taattcggga gccgccgaga aaactttcac gatcatggga
    10141 gcgaataatg aaaataaaaa tcctgaccat ttttggggtg acggttcttg cactggcgct
    10201 ttgcttttcc gtgagcacga ctcaggcatc gccgaagtcc accgcaacag cggcagtctc
    10261 agcaacatcg ccgcaaccgg cagccgcccc cgcgcccttg cctgaacctc atcccgaaat
    10321 c
//
LOCUS       DASZMB010000164         4010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_156721, whole genome shotgun sequence.
ACCESSION   DASZMB010000164 DASZMB010000000
VERSION     DASZMB010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156721"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..705)
                     /locus_tag="VG272_07440"
     CDS             complement(85..705)
                     /locus_tag="VG272_07440"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HWF13553.1"
                     /translation="MSSDDPIRAECKAQGLRTKRNNLLAAFLSGSILVFISLHFFPHT
                     IAGIAAGFLIGFFYANGFEYCLHRFLLHSGTGIFCQQHMVHHNTLQSPDAARYVNFSS
                     NPWGVVALFFANAVPFLFLQWAFHSAWTAGVFASFALYYIAFEEIHWRSHIGGWLPRW
                     VRPAARHHLMHHAHDSGRFNVFLPIFDWLAGLFQPRARSTATEARR"
     gene            924..1307
                     /locus_tag="VG272_07445"
     CDS             924..1307
                     /locus_tag="VG272_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWF13554.1"
                     /translation="MRHFRWAGLLCLAVFPVAAGAQQASADKQLTDAQKLGHRVFEQR
                     CGICHEQARAGFQTYAPLLYKDLVAGSEDAIKEIIRNGSAKMPGFKLGLQPAEIDAIV
                     EYLKTVPKPAKTTQPAAGGDMGPLD"
     gene            1326..3230
                     /locus_tag="VG272_07450"
     CDS             1326..3230
                     /locus_tag="VG272_07450"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HWF13555.1"
                     /translation="MKLFQSRSIVKFAGMAVALLALGAVLCSQAVAAPDGALLTGSVK
                     AADGTRLSGATVSAKLDDSTITTSVFTDEEGSYYFPSMGNGRYKVWAQADGFTIARSS
                     VDLGATKHEDFVLTPTKDVAKQLTGDQMLASLPDQTPDDRRLKRIFRNDCTSCHQPNY
                     ILQNQFDEKGWTAILEVMKRVNVAGGYAGANSPIAPQIDNHEKELAAYLARARGPEPS
                     SIANYKLRPRPTGDAARAIFTEYDVAKFAPNDAPKVYALNNGSDWSLGTPSSFFGNHG
                     VHDAQADFEGNIWFSINVPNAYASLGRVDAKTGETKFFRVDGIHGMAATGHGAYRDTQ
                     GHLWFNISAGEEKGPGRMIEVDPSAQTLQVYTPPNTMEGPTTVAGTVDVDGKGKVWAS
                     TFHGASRFDPVTKQFTEFKSPTFKNADGIGNTYGLTADGDGNGWWAEMNLDMVAKTDM
                     ETGKVVEIKIPPVPGIREMFSKEEAQAYDAGGSTWNSATPWAEGPRRMGADKQGHVVW
                     VCDWWGGSLAKIDTQTLKVTMIPLPRPDVQQPYQAAVDKDHNVWINLMNSDEVIKFDP
                     KTSKWTEYPFPTLGAEARYISLLEHNGKMEIILPYSRARRVARMTVRSKEEIQALKKQ
                     AQQQEQARAQ"
     gene            3530..3949
                     /locus_tag="VG272_07455"
     CDS             3530..3949
                     /locus_tag="VG272_07455"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HWF13556.1"
                     /translation="MAPFSAVRPVDGTVPANSSDKTETSSRGKVRSRKWIFVAGIVFF
                     SSLAVAQNSPQVTGVDPALGKVNDTVTVSGSNVGKDAVSAVYLSDDKNDYKGTIVDQS
                     SDKITMKVPQVKAGSYNISLQVGDKLFIKPVKFKVQE"
BASE COUNT          980 a         1039 c         1155 g          836 t
ORIGIN      
        1 tttcctatcc gcatagacag tcaaaacctg ccgccaagat gcctgcgcta taaaagccaa
       61 cccgccgcct cgcggtttcc tgatttatct tcttgcttcg gtcgctgttg atcttgcgcg
      121 cggctgaaat aatcctgcta gccaatcgaa aatgggaaga aagacgttga agcggcctga
      181 atcgtgcgcg tgatgcatca ggtgatgcct ggctgcgggg cgcacccatc gtggcaacca
      241 gccgccaatg tgcgagcgcc agtgaatttc ttcaaaggcg atgtagtaga gggcgaagct
      301 ggcaaaaacg cccgcggtcc atgcgctgtg aaacgcccac tgcaaaaata aaaacggaac
      361 ggcgttggcg aagaaaagcg cgacgactcc ccacggattg ctggaaaagt tgacgtagcg
      421 tgcggcatcc ggcgactgca gcgtgttgtg gtgcaccatg tgctgctggc agaagatgcc
      481 cgtgccggag tgcagcagga aacggtgcag gcagtattca aatccattgg cgtagaaaaa
      541 accgatcagg aaaccggcgg cgatcccggc aatcgtgtgc gggaaaaaat gcagagaaat
      601 gaaaaccagg atcgagccgg acagaaatgc agcgagcagg ttatttcgtt tggttctcag
      661 gccctgggct ttgcattcgg cgcggatcgg atcgtcggaa ctcatgactt gttaaagcta
      721 acgcgttgcg tgatttgccg caagcagcgg cgtttgttta agtagcacag gcttcagcct
      781 gtggggcttc gagcttgcaa ggactgaact ttttagtgat ttagctctcg ctccgactaa
      841 accccacagg ctgaagcctg tgctacttaa gccctgtttt ttggcctggg actcatgata
      901 gagtctgcga aggagacctt cctgtgagac attttcgatg ggctggcctg ctttgccttg
      961 cggtttttcc ggttgctgcg ggcgcgcagc aagcgagcgc ggataagcag ctgacggatg
     1021 ctcagaaatt ggggcatcgt gtgtttgagc agcgctgcgg catttgccat gagcaggcgc
     1081 gcgcgggatt tcaaacctat gcgccgctcc tgtacaaaga tctggtcgcc ggaagcgaag
     1141 acgccatcaa ggaaattatt cgtaacggat ccgccaaaat gccggggttt aaactgggct
     1201 tgcagccggc ggaaattgac gcgattgtcg aatatctgaa aaccgttccg aagcccgcga
     1261 agaccacgca gcccgccgcg ggcggcgaca tgggcccact cgactaagaa aaaggagagc
     1321 ctgcaatgaa actttttcaa tctcgctcga tcgtaaaatt cgcgggcatg gccgttgcgt
     1381 tgctcgcgct tggcgccgtg ctttgttcgc aggcggttgc agctccggac ggagcacttc
     1441 tgacgggctc cgtaaaagcc gctgatggaa cgcgcttgag cggagcgacc gtttcggcga
     1501 aattggacga ctcgacgatc acgacatccg tcttcacgga tgaagaaggg tcctactact
     1561 ttccgtcgat ggggaacggg cgctacaagg tgtgggcgca ggccgatggt tttacgattg
     1621 cgcgcagcag cgtggacctc ggagccacca agcacgaaga tttcgttttg acgccgacaa
     1681 aagacgtggc gaaacagctg accggcgatc agatgctcgc ttcccttccg gatcaaacgc
     1741 cggacgatcg ccgactgaag cgcattttcc gcaatgactg cactagctgc caccagccga
     1801 attatatttt gcaaaaccag ttcgatgaaa aagggtggac cgcgattctg gaagtgatga
     1861 agcgcgtaaa tgttgccggc ggctatgcgg gtgcgaacag ccccattgcg ccgcagatcg
     1921 ataaccacga aaaagaactc gccgcatatc tcgcgcgcgc tcgcggaccg gaacccagtt
     1981 ccatcgcgaa ttataaattg cgacctcgtc ccacggggga tgcggcgcga gccattttta
     2041 cggaatacga tgtagcgaag tttgcgccga acgatgctcc gaaagtgtac gcgttgaata
     2101 acggcagcga ctggtcgctg ggcacgcctt cgtctttctt cggcaatcac ggcgtgcatg
     2161 acgcgcaggc agattttgaa ggcaacatct ggttttcgat caatgtcccg aatgcttacg
     2221 cgagcctcgg ccgcgtcgat gcaaaaactg gagaaacaaa attctttcgt gtggacggta
     2281 tccacggaat ggccgccacc ggtcacggcg cgtacagaga cacgcagggc catctctggt
     2341 tcaacatttc ggcgggcgaa gaaaaagggc cgggacgcat gatcgaagtc gatccgtcgg
     2401 cgcagactct gcaggtttac actccgccca acacgatgga agggccgacc acggtagcag
     2461 gaaccgtgga cgtagacggc aaagggaaag tctgggcttc cacgtttcat ggagcttcgc
     2521 gcttcgatcc cgtgaccaaa caattcacgg aattcaaatc gccgacgttt aaaaatgcgg
     2581 acggcattgg caacacctac ggactgaccg cggacggcga cggcaacggc tggtgggcgg
     2641 aaatgaatct ggacatggtc gcgaaaaccg acatggaaac cgggaaggtt gtcgaaatta
     2701 aaattcctcc ggtccccgga atccgcgaaa tgttttccaa ggaagaagcg caagcgtacg
     2761 acgcgggcgg atccacctgg aattccgcga ctccgtgggc cgaaggcccg cgccgcatgg
     2821 gagccgataa gcagggacac gtcgtgtggg tctgcgactg gtggggcggc agccttgcca
     2881 agatcgatac gcagactttg aaggtgacga tgattccgtt gccccggccg gatgtgcaac
     2941 agccatacca ggcggcggtg gataaggatc acaacgtgtg gatcaatctg atgaactcgg
     3001 acgaagtgat caagttcgat cccaagactt ccaaatggac cgagtatccg ttccccactc
     3061 tcggcgcgga agctcgctac atttctttgc tcgagcacaa cggcaaaatg gaaattattc
     3121 tgccgtactc gcgggcgcgc agggtggcgc gaatgacagt gcgcagcaag gaagaaattc
     3181 aggcgctgaa aaagcaggcg cagcagcagg aacaggcgcg cgcgcaataa aagcggtaaa
     3241 agaagatttc aaattttaaa ttttgaaatt gagatttcaa atttcagatc tcgaaattca
     3301 acattgaaaa ctcaatttga gattccgatc tgaatttgag atttgaaatt tggcgctgaa
     3361 ttgcactaca ttatgaccgc cttatttctg cgcagctacc gctcccagtt tggctacttt
     3421 ctcacttccc tgtcgcacac tcattcatat tgacagcgcc tgagagcggg tgtaccgtcc
     3481 agcgcaagca gttcattttc aaatcgaaca ttcttgtgcg aggtgagtga tggctccttt
     3541 ttctgctgtc aggcccgtag acggcacagt tcctgccaac tccagcgata agactgaaac
     3601 aagctcccgc ggaaaagttc gttccagaaa atggattttt gtggctggaa ttgttttctt
     3661 ttcgtcgcta gcggtcgcac agaattctcc gcaggtgacg ggcgtcgacc cagcgctggg
     3721 aaaagtaaac gataccgtta cagtttccgg gagcaatgtg gggaaagacg ccgtatcggc
     3781 cgtgtatctt tctgacgaca aaaatgatta caagggcacc atcgtcgacc agagcagcga
     3841 caagatcacg atgaaggtcc cgcaggtgaa ggccggaagc tacaacattt cgctacaagt
     3901 gggcgacaag cttttcatca agcccgtgaa attcaaggtg caagagtagc gctagcatcg
     3961 ccactctgac gcggacgtaa ttgtctttca gtttctcaat tcacgaaagg
//
LOCUS       DASZMB010000165        22385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4839569, whole genome shotgun sequence.
ACCESSION   DASZMB010000165 DASZMB010000000
VERSION     DASZMB010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 22385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22385
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4839569"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(439..4779)
                     /locus_tag="VG272_07460"
     CDS             complement(439..4779)
                     /locus_tag="VG272_07460"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWF13557.1"
                     /translation="MNSQSLSWAHPIVQEWFAARFGAATEPQEQGWPHILAKKTTLIS
                     APTGSGKTLAAFLTCIDRLVRKAIAGELKDTTQVLYVSPLKALGNDIQKNLEQPLGEI
                     LQLAGARGFLMPEIRTAVRTGDTLMKDRRLMLKRPPHILVTTPESLYILLTAQKSREI
                     LKTVETVIVDEIHAVADDKRGTHLALSLERLEALTERPPVRVGLSATQKPIELVAHFL
                     AGTGRPDPVIVQVGHQRTIDLAVEVPRSELSAVTTHEMWDEIYDRLAQLIEQHRSTLI
                     FVNTRRLAERVAHHLAERLGKEAVATHHGSLARKIRLEAERKLKAGEVKALVATASLE
                     LGIDIGTVDLVCQLGTPRAISAAWQRIGRSGHWHGAIPKGRIFATTRNELIECAALVR
                     AMRQGDLDRISIPENSLDILAQQIVATCASEDWSEDDLFALVRRSHAYRNLDRSEFDE
                     VITMLCEGINARRGRYGAYLHRDRVNGKVRARRGSRLAAITSGGAIPDNALYAVIAEP
                     EGAHVGTLDEDFAVESLRGDIVLLGNNSWRIRRVESSRVLVEDAHGAAPNVPFWRGEA
                     PARTLELSQHVAEVRQKISTLTEGIPYRPPLRDFDSAAQAFRWLEDECGLDRSGAEQA
                     VEYIVAGKSVLTAVPTQQTIIAERFFDESGGMQLVIHAPFGGRINKAWGLALRKRFCR
                     SFNLELQAAATEEGLNIALSDQHSFPLADVFRFLHPASVEKVLEQAVLASPLFTTRWR
                     WDASRSLALLRFQGGRKVPPQIQRMRADDLLAAVFPDAAACQENIQGEMKIPDHPLIR
                     EVMKDALTEAMDVEGLKDLLHKILDGSIRCLAIDTPVPSQFSHEILNANPFAYLDDAP
                     LEERRARAVEMRRVLPETVASDMGRLDPAAIAEVRRDAWPDVRDAEELHDALDTLIAL
                     PIPAAAAQFDSENNSVNADAELHPLGRAVAQSIPEWQKYFVELASQCRAGKADVSGRT
                     YWVAAERSKLVATIFPGIIIDPLLAQVDQTESSKEEALYALLSGWLQHSGPVHARDLA
                     HLLGLAESETNNSLLRVEGSGAILRGQFTEARSSDIEWCERRLLSRIHRLTIGKLRKE
                     IEPVTAAQFMRWLARWQHVAPGTLTLGERGLLDVLRQLQGYEAPANAWESEILSRRVA
                     NYDPATLDNFCLKGIVGWGRLSPHPAMLHDRAERTRRIVPTSIAPITFFVRDAAEWMA
                     CRHHSAESETQKGLSRGAKEVLDLLAARGALFFADIVRGTKKLKSEVEADLWELVAAG
                     MITADGFDNLRALVDPKRRAGQGRGRTVRPRDSGGRWSLLFMDKVDDRNPAIESTCWM
                     LLNRYGIVFRELLTRESIELPWREILIALRRLEDRGEIRGGRFVNGFLGEQFALPLAA
                     DSLRASRSQPPTNEAIRIAAADPLNLVGIILPGERIPANSGRSFDLRDGVVVEQAPSA
                     ISALLSARAG"
     gene            complement(5007..5873)
                     /locus_tag="VG272_07465"
     CDS             complement(5007..5873)
                     /locus_tag="VG272_07465"
                     /inference="COORDINATES: protein motif:HMM:NF004824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HWF13558.1"
                     /translation="MALQKSDVWFITGCSTGFGRELAKFVLDRGYRAVITARDTGKIQ
                     DLKVGHEGRVLALKLDVTDRTEVEDAVRRAESAFGSIDVLVNNAGYGYVGAVEESDEA
                     EVRDLVETNFFGLARMIHVVLPGMRERRHGNIVNISSIGGLVGFPGVGYYNATKFAVE
                     GLSESLAKEVAPLGIKVLIVEPGPFRTDWAGRSLKKAGKQMPEYAETAGAFRERITGR
                     AGKQIGDPVRAAMAIVKALESDHTPLHLTLGQIALETARTKIEKLSAELDAWEDTSLS
                     ADFPESHAASAG"
     gene            6176..7138
                     /locus_tag="VG272_07470"
     CDS             6176..7138
                     /locus_tag="VG272_07470"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HWF13559.1"
                     /translation="MKAIVVHQFGGPEVLKLEEFPTPKAGPGQAVVRIHAAGVNPFDT
                     YMRNGTYAIKPALPYIPGGEGAGVVHEVGEGVTKFKAGDRVYVGHPVTGTYAEYSLAL
                     ESQLHSLPERTTYSQGAGIYVACGTAYHALHHHAQTHASETLLVHGASGGVGIAAVQI
                     ARAMGLTVIGTAGTDKGLELVRREGARHAFNHRTAGYTDEIMKATGGRGVDVVIEMLA
                     NVNLSADLKLLARFGRVIVIGSRGDVTITPRDLMARASAVRGFVLWSISEAHEADVHA
                     GLFAGLENGTIRPVVGKELGLGEAQRAHKEILETGALGKTILVP"
     gene            complement(7373..9148)
                     /locus_tag="VG272_07475"
     CDS             complement(7373..9148)
                     /locus_tag="VG272_07475"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HWF13560.1"
                     /translation="MPRKIATFFFVLSIAALTFMGLAQRNFAQSAPPSAVLSGTVEST
                     DGKPLGGIGVSARGANNTFTTTVYTDDAGRYLFPPMENGQYKIWAQAVGFEIFKGDAT
                     LSDGTEKQMEIKLATLTDFHKQLSGTEWAESLPEDTPDDRRMKTVFINNCSGCHQVSF
                     LLQNRFDAAGWAAVITLMEKMQSIGYAPSDIAPNAVIHSYKPELAAFLGRVRGPSNAT
                     LYWKLLPRPTGDAAKIVITEYDLSRPDIPGWTMEHNGTDWSEGTPSRWDGRAAHDVAV
                     DKGGFVWFADDATPGRTLAKLDPSTGKITDYVLADQNNAAESSHALVFDKAGNIWFAN
                     GTEGSPTKFDPETGKFFRYPRPKDFPFSGDFITMDPKGNVWSPHRQGAYKLDPVTGKY
                     TNFALGPGKANYDIAADDEEKVWISQPGGNDMEMVDSRTGKIERLDLNPVISQEYEVT
                     PKDRELAASLDLTPNTATPLDKGPRRSSADRDGDFIWVCEFFADRLAKIDARTKKVTE
                     YSLPHRFSQPYSATVAKDHTVWITMLNSDRIAKFDPATEKFTEYVLPTRGTEIRHIQV
                     DSSTNPPTVWLPYDRTNKIARIQFR"
     gene            complement(9177..9551)
                     /locus_tag="VG272_07480"
     CDS             complement(9177..9551)
                     /locus_tag="VG272_07480"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWF13561.1"
                     /translation="MKITSMMVVVLALSCASVSGFTVLRAQNKPSAVVPGAAKLSAEA
                     TRGEGLFLQRCSICHLPRKLKFGSPPVIGPSLTGQFKSATPDQMKVLRGFILKGGPDM
                     PGFQYGLEPNEVEDLIAFLKTL"
     gene            9834..10439
                     /locus_tag="VG272_07485"
     CDS             9834..10439
                     /locus_tag="VG272_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13562.1"
                     /translation="MNRILIATLLFVGMAGNISAQNIPKWDLYGGYSEYLAGSGIAEQ
                     GQLSSNGVQVDVDRVFTSYFKVSSQFNAQFGDHIVNIAPLTSPGAQHVNSKELLLLFG
                     PEATYRRLKRFDIFAHYLIGLSYGRDNEYPKIPTATYTTWAYALGTGVDLKLGSKVSA
                     RLLGFDWITTHFPINRPEAQDNWRLTSGFVFHFGESSNAEK"
     gene            complement(10461..11108)
                     /locus_tag="VG272_07490"
     CDS             complement(10461..11108)
                     /locus_tag="VG272_07490"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HWF13563.1"
                     /translation="MISFPRMSKYECILFLFLFSWMISGPPAAHAQASNSPETADRLP
                     VRGTEIEVWTGGGADPIGSNGVTQGNSQWTAGLRYGWILTDAHGPGFLRGRFEYALDA
                     MPVVMVFQPGGHAYGFGFDPWIMKWNLEPHHRISPYIELGGGGVISTREIPMGESRFN
                     FMPTGALGVNILRGKYHWSIDFRYLHFSDAQITSFNPGTDTFGLRIGWGVFTPSH"
     gene            11525..12562
                     /locus_tag="VG272_07495"
     CDS             11525..12562
                     /locus_tag="VG272_07495"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HWF13564.1"
                     /translation="MHTRASDGSGTVADVMSAAEKAHLTFVAITDHNVAMTQTELNED
                     PPDLPIISGEELGTNAGHFLALGIPASWVEPKTKDDRELLAAARSVGAFRVLAHPFHP
                     HTPWTDWGTTDFDGIEIWNEDATWRQNNFFDLVNALIIYGENNQLALVRLARTPDENF
                     AKWDELLQQRPVAGICAADTHARIRLAFGKDWKFPQYVPSLEVAREHVLISPDAGGGN
                     PALASASEIVDTLRNGHSYCGLDSLYPADGFSFRISNANGSGGPGDVLRSTGSARLQI
                     SVPAGSSLPLIKIFRDGREIDEQETRTIDEPVSGSGRYRTEVFLRQPGFTGWRRWTLW
                     VFTNPIYVTTR"
     gene            12706..14373
                     /locus_tag="VG272_07500"
     CDS             12706..14373
                     /locus_tag="VG272_07500"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HWF13565.1"
                     /translation="MNKTFSSVFLAFLFTFAISSYASPGGDISGTVKAEDGSPFRAAF
                     VRAQNVKTKMIMMVLTNNQGQFRTDNLPAGAYEVWATSTGYRSDPSRRTDITVQDGKT
                     TSATFTMKKGSVQWSQLTKYQAGILLPDLKGKDVVLQECFNCHAMSRIGMMGRDREGW
                     LDAIEHMRQVGVADITPKVAEQVSEYLGTVFGPDAETPSSPAQLPQYQKVKQEHDYFS
                     DDALNIVYVDYQLTGEPKDRPGSSKQDKDGNMWMEMGGGLSKLNPETGELHTWRLPDA
                     TRPFIHEILPTSDGYVWLTIEKQNALARFDTRTEKFEVYRQPYDGPPPAEMEPNAPWP
                     NLRYTAGGQTSDPRRHTAVADLQGNIWSSGRPLSKFDIETKKYTFFPEVPDTYGIAVD
                     KEGNVWFAEFNSRDHRSIGKVDVKTNKVTKWTPPNSETRPRRLKIDSQGMIWFGDYFG
                     GNLTRFDPKTQQFKEFKLPGPMPTPYGISVDHNDELWYASMYTDVIGKLDPRTGKVIE
                     YPTPYGERGTRDMFEDAQGRIWYGAQPYGKVGYVRLRKETEKAVAQK"
     gene            complement(14446..15297)
                     /locus_tag="VG272_07505"
     CDS             complement(14446..15297)
                     /locus_tag="VG272_07505"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HWF13566.1"
                     /translation="MKQIKTAKTETTRRAFISKSGLLLAGTVLASSSAVEVKAKSENF
                     TPPIPDPQQDVVTLRADLATANHILYDQGVVDGYGHVSVRHPGNPNHFLISRWLAPDM
                     VTEADILEVDFDCKPVGSDKPRLYSEVFIHGEIYRARPDVKSIVHTHAPAVILMGISG
                     ETLMPMYQMTGYMGAGVPLFDIRQAVGMTNMLISDPARAKALVQKLADKPAVLMRGHG
                     GVVTGASLAQAVGRSVYMRVNAELQVQVLGKKMTYLAPEEAQLTSNNDYPKDWEMWKR
                     KVAGQRG"
     gene            complement(15326..16213)
                     /locus_tag="VG272_07510"
     CDS             complement(15326..16213)
                     /locus_tag="VG272_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13567.1"
                     /translation="MKCSKARDHANGFGPLFPKIVLLLSIFPWLAGISARATELKPET
                     VAAFNHYVEITESKMQTDLDGNRFLVIDRLPEAQCKHAYEQLHRGEIYIQEMHTVDGH
                     NSIHIPNGLIHHWAGVIFIPKGKLKDVNAVLHDYANEPRIYEPEIRRAKLIEQNGNTS
                     KVYLQFFSKSIITVVLDGYFEVTLKSIDDTRSESISHSTRIAEVANPGEPDEHVRAEG
                     SDHGYMWRLNSYWRIEEKDGGVYVQNESITLTRTVPIMLAWIVNPLIKSLPRDVLAHM
                     LNDTQDAVLKRSKVSTVAP"
     gene            complement(16394..17023)
                     /locus_tag="VG272_07515"
     CDS             complement(16394..17023)
                     /locus_tag="VG272_07515"
                     /inference="COORDINATES: protein motif:HMM:NF017778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13568.1"
                     /translation="METSQRSAIISPRIERLRDFIISFSALMERTHEEPEILRNGSRL
                     LKELVGVDDWLPDRYAQASESGYQQYLLHADSQERFSVVSFVWGPAQKSPIHDHTVWG
                     IVGILRGAEFEQSYSRVSDGRFVQHGAPERLAQGEVTAVSPSVGDFHQVSNALADQPS
                     VSIHVYGANIGAVQRSMYELDGTQKTFRSGYSNAEMPNLWDRSKDRTVS"
     gene            17580..18428
                     /locus_tag="VG272_07520"
     CDS             17580..18428
                     /locus_tag="VG272_07520"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF13569.1"
                     /translation="MTIDIHPHVISTDIQRYSNAPVGGHKSDWSRERPVSVEQMIVAM
                     DQAGIAKSALVQASTCYGHDNSYIADAVAAHPTRFTGVFSVDVLAPDAPEKIRYWVGR
                     NLTGMRLFTAGSTMPNQADWVDDPRSFPAWECASELGIPVCVQMTVKAIPQLVRMLER
                     FPKVRAILDHLAKPTLSDGPPYAAAAAVFELANYKNLYLKLTPRTAAEAQNGKATLAT
                     FFPLLVSKFGASRIAWGSNYPASEGTLSELLTVCQSALSVLSAEDHDWIFAKTALTLY
                     PALAGK"
     gene            18438..19328
                     /locus_tag="VG272_07525"
     CDS             18438..19328
                     /locus_tag="VG272_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13570.1"
                     /translation="MLETPHPLTLQTLLTSHANTSALRSGQLQSPLVNFVFADIKPAN
                     RGFKPLVREQKFDLGELAIVTFLQAKTYGNPYVLMPAVVVARGQHHTIFFNPQRGHLN
                     PGDLNGRRVGVRAYTQTTGAWVRGFLQEDYGVDVRTIQWITFEDPHVAEYADPSWVQR
                     APEGKQLLQMLIDGEIDAAIFGSENPEGTPLKPLIPNAAEVAARWAQKHGGAPINHMM
                     VIKESITQTRPDVVREVYRLLKESAAAAPAKETSALRFGVESVRKSLETIIQYAEGQN
                     LIPRRFSVDELFNDVTRSLD"
     gene            19337..20674
                     /locus_tag="VG272_07530"
     CDS             19337..20674
                     /locus_tag="VG272_07530"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HWF13571.1"
                     /translation="MQPPATKLDETDASKAHVVPRARAWYKHLSIQILFAMILGVIVG
                     HTWPQHAEAWKPLGDLFIKLVRMLVAPIIFCTVVHGIASVGEAKKVGRIAIKSLIYFE
                     IVTTIALVIALLLVNFWAPGANMHVDLKTLADIGVKAPAKPITFGQFLLNLVPSTAVG
                     AFAEGDVIPVLFFSLMFAFGLLALGAKGKPLIETIHSVTQVLFKMIGFAMYLAPIGAF
                     GAIAFTVGKFGLVSLLALGKLVLEFYVCCLLFVAIVLTPIAYVAGVNILRLIRYFAAE
                     LMIVVGTSSGESVFPSVMAKLRGLGIEESTVALVLPTGYSFNHDGTCLYWATVSVFLA
                     QAVGIHLSIGQQLALLATMLFTSKGGAGVAGSAIVILATTLSATNVIPVASIGLILGV
                     HSLLSSAFVPVNVLGNSLATIVIARTERAVDLQTLQSQLKSGPEETTQVTSAF"
     gene            complement(20806..21792)
                     /locus_tag="VG272_07535"
     CDS             complement(20806..21792)
                     /locus_tag="VG272_07535"
                     /inference="COORDINATES: protein motif:HMM:NF028132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13572.1"
                     /translation="MGNLLRFRQYHREVFTTDRFGFRNLPSEDADEMTSAILVGDSFA
                     VGASNSDGDTLPEQLTSRLSNGRVYNGGNAGPHWKMVRELIKRLHMRGGLVIWQVSER
                     QKIPASLQAEGPYNPGVAPSAFGERRSILQRFSVWADGLLAYSPLRVFLSGGYRRLEN
                     GVLFPNPSENLVMVGHLHNGDSMLFLRTEVDNFYQPKYDSPTYLSEINSLVHCTGNDL
                     LVLMVPDKFGVYYPLLRDGLQLPPEGKSHLDKLEEDLRRSGVPVLNLMGPLRSQAAES
                     LQKREYNYRLDDTHWNPAGIQTAATGILRAWSNPPGNINNASISHNSCPAVQ"
     gene            complement(22035..>22385)
                     /locus_tag="VG272_07540"
     CDS             complement(22035..>22385)
                     /locus_tag="VG272_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13573.1"
                     /translation="RYSFRRIVFVPAWLRRMFTFFLVVIGWVLFRATSFTMAAGWLRK
                     MFVWNPESGDLGAWTLVLPILIAGGLAHCCPNSFELSHRWNWASALALTALFAACLVA
                     IYGGKATPFLYFQF"
BASE COUNT         5259 a         6008 c         5940 g         5178 t
ORIGIN      
        1 cgaatccaga ggggtttctg ttcgtaacaa gaaacagaag acccccttcg tcaaacaaag
       61 tcgtggagtt tgggctttgg ccagtgctcg atctcttggc catcccccgc tgtgggctcc
      121 atgcattccg acacactcac acgagcttgc tactcgacac gggcgcaact ccgaaggtcg
      181 ttcaggaaca gttacggcac gcagatcccc gcgtcactct tggggtctat gcccacgttc
      241 tcggtgatgc tcaccgcgat gcagtcgaga aagtagcctc ggttgtgttc caaagtgttc
      301 caaaggcgag agaagaaacg aagtatattc agtagcttcc aggcaatcgg cgttgggttc
      361 gattcccatc gcccgctcca gaaagcctca aaattccttc cagggtccaa ccaccgacgt
      421 ttagagggtt gaatgcaatt atccggcgcg ggcgctgagc agggctgaga tggcgcttgg
      481 agcctgttcc acaaccacgc catcgcgcaa atcgaaggag cggcccgaat tcgcagggat
      541 tcgctcgccc ggcagaatga tgccgaccag atttagagga tcggcggctg cgatgcgaat
      601 cgcttcgttc gttggtggtt gagaccgcga cgctcgcagt gaatctgccg caagtggaag
      661 cgcgaattgc tctccgagga aaccgtttac aaatctgccg ccacgaattt cgccgcgatc
      721 ttcgaggcgg cgcagcgcga tgagaatctc gcgccaggga agttctatcg attcgcgcgt
      781 cagcagttcg cgaaatacaa tgccgtagcg attcagaagc atccagcagg tggactcgat
      841 tgcgggattg cggtcgtcca ctttatccat gaacaggagc gaccagcgtc cgccgctgtc
      901 gcgcgggcgg acggttcgtc ctcttccctg gcccgcgcgg cgcttgggat cgactagcgc
      961 gcgcagatta tcaaaaccat ccgcggtaat cattccggcg gcaaccagtt cccagagatc
     1021 ggcctcgact tcggatttta atttttttgt tccgcgcacg atatcggcaa agaataaagc
     1081 tccacgcgct gcgagcaaat ccaaaacttc tttggcgccg cggctcaaac ctttttgagt
     1141 ctccgattca gcgctgtgat gacggcaggc catccattcg gcggcgtcgc gcacaaaaaa
     1201 agtaatgggc gcgatgcttg tggggacgat gcgacgcgtg cgctcggcac gatcgtgaag
     1261 catggcgggg tgcggcgaga gccggcccca gccgacgata cctttcaggc aaaaattatc
     1321 cagggtcgcc ggatcgtaat tggcaacacg gcggctgaga atttcgcttt cccaggcgtt
     1381 ggcgggggct tcgtaaccct gcaattggcg caacacatcg agcaacccgc gttctccgag
     1441 cgtcagcgtt ccgggagcaa cgtgctgcca gcgcgctagc caccgcatga attgcgccgc
     1501 ggttaccggt tcgatttcct tgcgaagttt tccgattgtg aggcggtgaa tgcgtgagag
     1561 caaacgtcgc tcgcaccatt cgatgtcgct agagcgtgcc tccgtaaatt ggccacgaag
     1621 gatcgctccc gatccttcga cgcgcagaag cgaattattg gtctcggatt ccgcgagtcc
     1681 cagcagatgc gcgagatccc tcgcgtgcac tgggcctgaa tgttgcagcc aacccgacaa
     1741 gagggcgtac aacgcttctt cctttgacga ttcggtttgg tccacttgcg ccagcaacgg
     1801 atcaataata attccgggga aaattgttgc gactagtttt gaacgttcag ccgcaaccca
     1861 gtatgtgcgg ccggagacat ctgcttttcc cgcgcggcat tgtgacgcaa gctcgacaaa
     1921 atatttttgc cattcaggaa tggattgagc aacggcgcgc cccagcgggt gaagttcggc
     1981 atctgcgttc accgaattat tttctgaatc aaactgagcg gcggcagcgg gaataggcag
     2041 ggcgattaac gtgtcgagcg cgtcatgcag ttcttcggcg tcgcgcacat ccggccatgc
     2101 atccctgcga acttccgcga tagccgctgg atcgaggcgc cccatatccg acgcgacggt
     2161 ttcaggaagc acgcgacgca tttccacggc gcgcgcgcgg cgctcctcaa gcggggcgtc
     2221 atccagatac gcaaacggat tggcattgag aatttcgtga gaaaactgtg aagggactgg
     2281 cgtgtcgatc gcgaggcaac gaatgcttcc atcgagaatt ttgtgcaata aatccttcaa
     2341 accttccacg tccatcgctt ccgtgagcgc atctttcatc acttcgcgaa tgagcggatg
     2401 atcgggaatt ttcatttcgc cttgaatatt ttcctggcat gccgctgcat ccggaaaaac
     2461 ggcggccagc aaatcatctg ctcgcatgcg ctgaatttgc ggaggcactt ttcgtccgcc
     2521 ttgaaagcga agcagcgcga gggagcgcga agcgtcccaa cgccagcgcg tggtgaagag
     2581 cggcgaagcg agcacggcct gctccagaac tttttctacg gaggcgggat gcagaaatcg
     2641 aaaaacatcc gcaaggggaa agctatgttg atcggacaaa gcaatgttca ggccctcttc
     2701 cgtggccgcg gcttgcaact caagattgaa ggagcggcaa aaacgcttgc gcagcgccag
     2761 gccccaggcc ttattaattc gtccaccgaa tggggcgtga atcaccagct gcattccgcc
     2821 gctttcatca aaaaagcgtt cggcgatgat cgtctgctgc gtgggaacag cggtgagcac
     2881 ggatttgccc gcaacaatat attccacggc ttgctccgct ccggagcggt ccagaccaca
     2941 ttcgtcttca agccagcgaa atgcttgagc ggcggaatcg aaatctctca gtggcggacg
     3001 atacggaatt ccttccgtca gggtggaaat tttttgccgc acttcggcta cgtgctgcga
     3061 aagttcgagc gtgcgtgcag gagcctcgcc acgccagaac ggaacgtttg gagccgcgcc
     3121 atgtgcgtct tcgacgagga cgcggctcga ctcaacgcgc cggatgcgcc aggaattatt
     3181 tccgagaagc acgatgtcgc cgcgcaaact ttccacggca aaatcttcgt cgagcgtacc
     3241 aacgtgagcg ccttccggct cggcgatgac ggcgtaaagc gcgttatctg gaattgcgcc
     3301 gccgctggta atcgcagcga ggcgacttcc gcgccgcgcc cggacctttc cattgacgcg
     3361 atcccgatgc aaatacgcgc cgtaacggcc acgtcgcgca ttaatcccct cgcaaagcat
     3421 cgtaatgact tcatcgaatt cactgcgatc cagattgcga tacgcgtggg agcgccgcac
     3481 caaagcaaag aggtcgtctt cactccagtc ttcggacgca cacgtggcca cgatctgctg
     3541 cgccaaaata tccagagaat tttccggaat gctgatgcga tcgagatcgc cttgccgcat
     3601 ggcgcgcacg agtgccgcgc attcgatcag ttcgttgcga gtggtggcaa atatacggcc
     3661 cttcggaatt gcgccatgcc agtggcctga tctgccaatg cgttgccaag ccgcggaaat
     3721 ggcgcgcggc gttccgagct ggcaaacgag atcaactgtt ccaatgtcga ttcccagttc
     3781 gagtgaagca gtggctacaa gcgcttttac ttctcccgct tttaattttc gctctgcctc
     3841 aagccgtatc tttcgtgcca ggctgccgtg atgcgtggcc acagcctctt ttcccaatcg
     3901 ctcagccagg tggtgcgcca ctctttcggc gaggcggcgc gtgttcacaa aaataagcgt
     3961 tgaacgatgc tgttcgatca gttgcgccag acgatcgtaa atttcgtccc acatttcgtg
     4021 cgtggttacg gcgctcaact cgctgcgcgg aacttccacg gcgagatcga tcgtgcgctg
     4081 atgtcccact tgcacgatca caggatccgg ccggccggtt cctgcaagaa aatgagcgac
     4141 caactcgatt ggtttttgtg tggcggataa acctacgcgc actggaggac gttcggtcag
     4201 cgcctccaaa cgttccagag ataaggccaa gtgcgttccg cgcttgtcgt cggcaacggc
     4261 atgaatctcg tcgacaatca cggtctccac ggtcttcaga atttcccggc ttttttgtgc
     4321 agtcaacaaa atgtaaagag actccggcgt ggtcactaga atgtgcggcg ggcgctttag
     4381 catcaggcgg cggtctttca tcagggtgtc gccggtccgc acagccgtgc gtatttccgg
     4441 cataagaaaa ccacgcgcgc cggcaagctg gagaatttca ccgagaggtt gttccaggtt
     4501 tttttgaatg tcattcccta aagcttttag aggagagacg tacaggactt gcgtcgtgtc
     4561 tttgagttcg cctgcaatcg cctttcgcac caaccggtcg atgcatgtga gaaaagcggc
     4621 caaagttttt ccagatcccg tgggcgcgga gatcagcgta gtttttttag cgaggatgtg
     4681 cggccagccc tgttcctgtg gctcagttgc ggcgccaaaa cgcgccgcga accattcttg
     4741 aacgatggga tgggcccagc tgagggattg cgaattcata gatgctcgtc gaccttatag
     4801 acgctcagtg acacggacgg ctggagaaca aaaataaatt atacaccttc ggacaagacc
     4861 aggcagacgc gccaacagct catcatcaaa gcaaattatt tttgatttgc catgcgggac
     4921 ttgagacggc aagaaatctg cgctgatgaa ataaatgcgt cgcccaaacg cagatgttgc
     4981 tcgtgtgttt ttatcaggaa aagcttctat ccggccgatg cggcgtggct ctccggaaaa
     5041 tccgcgctga gcgaagtatc ctcccaggca tccagctccg cgctcaattt ttctatttta
     5101 gtgcgggcgg tttccagtgc gatttgtccg agcgtcagat gcagcggcgt gtgatcggac
     5161 tcgagcgcct tcacaattgc catcgccgcg cgaacgggat cgccaatctg tttgccggct
     5221 ctgcccgtga tgcgctcgcg gaaagcgccg gcggtttccg catattccgg catttgcttg
     5281 ccggcttttt tcagcgagcg gccagcccaa tccgtgcgga acgggcccgg ctccacgatt
     5341 aaaactttta tgccgagtgg cgcgacttct ttcgccagcg attcggagag cccttccacg
     5401 gcaaatttcg tggcgttgta atagcccact cccggaaatc ctacaaggcc gccgatggac
     5461 gaaatattta cgatattgcc gtgccgccgt tcccgcatac ccggcagcac cacatgaatc
     5521 atgcgcgcga gaccaaaaaa atttgtttcc accagatccc gcacctcggc ctcatcgctt
     5581 tcctcgactg cgcccacgta accataaccc gcgttgttca ccaggacatc gatgctgcca
     5641 aaggcagact ctgcgcgcct cacggcatct tccacttctg ttctatcggt gacgtctagt
     5701 ttcaaggcca gtacgcggcc ctcatgcccc actttcaaat cctgaatttt gcccgtatcg
     5761 cgggcggtaa tcacggcgcg ataaccgcga tcgagcacga atttggcaag ctcacggcca
     5821 aagccggtgg aacatcctgt aataaaccaa acgtctgatt tttgcagtgc catggctata
     5881 tctcctgcgg gggcgctggc acatcccggt gtcatgggtc cgcgcatcca gaatatacac
     5941 ccgatagcga taaatttgcg agccgcatcg cacctgaaat acgaggattg gtggataggg
     6001 cttcaaagtc tgtgtgagaa ctgcaacatt ggaacctcag cggctaaata ccggactcat
     6061 tttgcaggtc ttttgtcgtc actgaagccg gtgctaccgg tagcgcattg acattgattg
     6121 ctcgagtggc cagaatttgg gacgggacat aattcaagtg acggagaaaa ccaagatgaa
     6181 agcgatcgtc gtgcaccagt tcgggggtcc agaagtccta aagctcgaag aatttcccac
     6241 gccaaaggcg ggccctggac aagcggtggt acgcattcat gcggcgggcg tgaacccgtt
     6301 cgatacttac atgcgaaatg gcacctatgc tatcaagccg gcgctgccct acatcccggg
     6361 cggagaggga gccggcgtgg tgcacgaagt tggcgaaggc gttacgaaat tcaaagcggg
     6421 tgatcgcgtt tatgtcgggc atccggtcac tggcacgtat gcggaatact cactcgcgct
     6481 cgaatcgcag ttgcacagcc tgcccgagcg cactacttac agccagggcg cgggaattta
     6541 cgtggcgtgc ggcaccgcgt atcacgcgct ccaccatcac gcgcagacgc acgcgtcgga
     6601 aaccctcctg gtgcacggcg cgagtggcgg cgtgggaatc gccgctgtac aaatcgcacg
     6661 cgcgatgggc ttgacggtga tcggaaccgc gggaacggat aaaggattag aactcgtgcg
     6721 acgagaaggc gcgcgtcatg cgttcaatca ccggactgcg ggatacacgg acgaaataat
     6781 gaaggccaca ggtgggcgcg gagtggatgt cgtcattgag atgctggcaa atgtaaacct
     6841 ttccgcggac ttgaaactgc ttgcgcgatt tggacgtgtc attgtcatcg gcagccgggg
     6901 tgatgtgacg attacgccgc gtgatctgat ggcgcgtgct tcggccgtgc gtggcttcgt
     6961 tctctggtct atttcggagg cccacgaagc ggatgtccat gcggggctct tcgcagggct
     7021 ggaaaatgga accattcggc cggtggtcgg taaagagtta ggactgggag aggcacagcg
     7081 cgcgcataaa gaaattctcg agaccggtgc gctgggaaaa acgattctgg ttccgtagaa
     7141 ctgtgccagg aaaagaaatg agagatggag agcaggcttc agaatctgtg tgagaactgc
     7201 aagatgggag cctcagcggc caaagccgga ctcatttgca gaacttatgt cgtggctaaa
     7261 gtcacgaccc acaacaattc tgcaattctc actcagactc ttcagcttgt ttgatttttg
     7321 cagatgatga aaaaatcaaa agagaacagg ctgaagcctg ttctacttag acctacctga
     7381 attgaattct ggcaattttg ttggtgcgat cgtaggggag ccagacggtt ggcggattcg
     7441 tgctgctgtc gacctggatg tggcgaattt ccgtgccgcg agtgggaaga acgtactccg
     7501 tgaatttttc agtcgctgga tcgaacttgg cgattctgtc cgagttgagc atggtgatcc
     7561 atacggtgtg atccttggcg acggttgcgg aatacggctg gctgaagcgg tgaggcaatg
     7621 aatattccgt caccttttta gttcgagcat cgattttggc aagccgatcc gcaaaaaatt
     7681 cgcacaccca gatgaaatcg ccatcgcggt cagcggaaga tcggcgcggt cctttgtcga
     7741 gcggcgttgc ggtattcgga gtaaggtcca ggctagcggc aagttcacgg tctttgggtg
     7801 taacttcgta ctcctgcgaa atgaccggat tcaaatccaa ccgttcgatt tttccggtgc
     7861 gcgaatccac catctccata tcgttcccgc cgggttgcga aatccagact ttttcttcgt
     7921 catcggcggc aatatcgtaa ttcgctttgc caggaccgag cgcaaaattc gtatatttcc
     7981 ctgtcaccgg gtcaagcttg taggcgccct gccggtgagg cgaccacaca ttccccttcg
     8041 gatccatggt tatgaaatcg ccggaaaatg gaaaatcttt tgggcgagga tatctgaaaa
     8101 attttccggt ctccggatcg aattttgtcg ggcttccttc tgtgccattc gcaaaccaga
     8161 tattccctgc cttatcgaag accagggcgt gggaactttc cgctgcgttg ttctgatcgg
     8221 ccagcacgta gtccgtgatc ttcccggtag atggatcaag ctttgctaaa gtgcgtcctg
     8281 gcgtcgcatc gtcggcaaac cagacgaagc cgcctttgtc gacggccaca tcgtgtgcgg
     8341 ctcgcccgtc ccaccgcgaa ggcgttccct ccgaccaatc cgtcccgttg tgctccatgg
     8401 tccagcccgg aatatccggg cgcgacaaat cgtattcggt aattacaatt ttggcagcat
     8461 ccccagtggg acgcggaagg agcttccaat acagcgtggc gttcgatggg ccgcgaacgc
     8521 gacccagaaa agccgcgagc tccggcttgt aagaatgaat gacagcattg ggagcgatat
     8581 cggacggcgc gtatccgatc gactgcattt tttccattaa agtaatcacg gctgcccagc
     8641 ccgccgcgtc aaaacgattc tgcaacagga agctgacttg atggcatcca ctgcaattat
     8701 tgataaatac ggttttcatc cggcggtcat cgggcgtatc ttcgggcaaa ctctcagccc
     8761 actcggtgcc ggaaagttgt ttatgaaaat cagtaagagt tgccagcttg atttccattt
     8821 gcttctcagt tccatccgaa agagtggcgt cacctttaaa aatttcgaag ccgaccgcct
     8881 gcgcccaaat tttgtactgg ccgttttcca tcggcggaaa caaatagcgc cccgcgtcgt
     8941 ccgtatagac cgtggttgta aaagtgttgt tcgcaccgcg cgcggaaacg ccgattccgc
     9001 cgagtggttt tccatctgta gactcaacag ttcccgaaag gactgccgaa ggtggcgcag
     9061 attgcgcaaa gttcctctgc gcaagaccca tgaatgtaag ggcggcaatg gataacacaa
     9121 aaaagaaggt tgcaatcttt cgcggcaaaa gctcctccaa ttaaaaaaac cagcctctat
     9181 agagtcttca agaatgcaat caaatcttcc acttcattcg gctccaaacc atattggaat
     9241 ccgggcatat ccggtccgcc cttcaaaata aagcctcgta ggactttcat ctgatcggga
     9301 gttgctgact taaattgccc ggtgaggctc gggccaatca ccggcggaga tccaaattta
     9361 agttttctgg gcaagtggca aatagagcag cgctgaagaa acaatccttc tccccgcgtc
     9421 gcctcggcgc tcaacttggc agcaccgggg acgactgcgg agggtttatt ctgcgcgcgt
     9481 aatacggtga aaccacttac ggaagcacag gaaagagcca gaaccacaac catcatcgat
     9541 gttattttca aaataagttt taatctcccg tcaaaactct ggaacttgta ccgcacacaa
     9601 tcggatggcc ccttctgtac tgccacgcgg cgctactgtc aagaaataag atacgctaat
     9661 ccgcaatgaa ttttgcatta ctttgcaatc cggatgcatg gatggaacga caaaaaggcg
     9721 ggacgtatca tccaatctct agcaatctcg cgcgccttct gaaaattttt ggagggcatg
     9781 ggaagtgttt tttggatttt gcaataaatc caatccaagg gagcccggga caattgaaca
     9841 ggatcctgat cgctacgttg ctttttgtgg ggatggcggg aaatatttcc gcgcagaaca
     9901 ttcccaaatg ggacctctac ggcggatact cggaatatct cgcaggttca ggaattgctg
     9961 aacagggaca actgtcaagt aatggtgtgc aggttgatgt ggatcgagtt tttacaagct
    10021 atttcaaagt ttcaagccag tttaatgccc aatttggcga tcatattgta aatattgctc
    10081 ctcttacttc tcctggcgcc cagcacgtta acagtaaaga gttactcctc ttattcgggc
    10141 cagaagcaac gtaccgccgc ctgaaacgat ttgatatttt cgctcattat ttaattggcc
    10201 tttcctacgg acgggacaac gaatacccca aaattcccac ggctacctac acgacatggg
    10261 cctatgctct tggcactggc gtggacctga aactcggcag caaagtttcc gcaaggctgc
    10321 tgggattcga ttggatcact acacacttcc ctattaaccg tccggaggcg caggataatt
    10381 ggcgactcac cagcggattt gtatttcatt tcggagaatc atcaaacgcg gaaaaataat
    10441 agtgcgcgtg gatgcagtat ctaatggctg ggagtgaata caccccagcc gattcgcagc
    10501 ccaaaagtat ccgtgccggg attaaagctg gtaatttgtg catcggagaa atggagatat
    10561 cggaaatcga tgctccagtg atattttcct cgcaggatat tcactcccag cgccccagtg
    10621 ggcatgaaat taaatctgga ttcgcccatg ggaatttctc tggtactgat cacgccaccg
    10681 ccacccaatt cgatgtaagg tgaaattcga tggtgcggtt cgagattcca tttcatgatc
    10741 cacggatcga acccgaaacc gtaggcatgt ccaccaggct gaaagaccat aacgactggc
    10801 atggcgtcca gcgcatattc gaatctgccg cggagaaatc ctgggccgtg cgcgtcggtg
    10861 agaatccagc catagcgcag accggcggtc cactgactat tgccttgggt cacgccgttc
    10921 gagccaatag gatcagcgcc gccacctgtc cagacctcaa tttcagtgcc gcgcacgggg
    10981 agtcgatcgg cggtttccgg ggaatttgaa gcttgggcgt gagcggctgg cgggcctgaa
    11041 atcatccatg aaaataaaaa gagaaacaaa atacattcgt acttactcat ccgagggaag
    11101 gaaatcaatc gtttctcctt atcctgctca caactcactc taacatataa ttaaatctcc
    11161 tcaacggagc caggctcctt gaagttgagt ttgattttca aatttgttaa atcgtgactg
    11221 cttatgtatc tgtttgagga caagatcgat tgctgcttga cagcctcccg ataggtgttc
    11281 acacttttac tgggcatctc tactgttacg tccaaaagat ggatttttcc gcccggcttt
    11341 tcctcagccg acaaaggagg cacacatctt cggtccggta ccaaaaagta aattaaaact
    11401 atggttaact ttcttcttcg ctgcaacctt gattgtcgcg ggaattctgg ccgatggtct
    11461 gctcgtcagg cttccgcgta tttcaacgga taaagtggcc tcagaacgag ttggggtact
    11521 ccatgtgcat acgagggcgt ccgacggaag cggaactgtg gccgatgtga tgtcagctgc
    11581 cgaaaaagcg catctcacat tcgttgcaat caccgatcat aacgttgcca tgacccaaac
    11641 cgaactgaat gaggatcccc ctgatctgcc catcatttcg ggagaagaac taggcacgaa
    11701 tgcgggacat ttcctggcgc tgggcattcc cgcgtcatgg gtggaaccga aaacaaagga
    11761 cgatcgcgaa ctacttgcag cggcaagatc cgtgggagcc tttcgggttc ttgcacatcc
    11821 ctttcatccg catacaccat ggaccgactg ggggacgacc gactttgacg ggatagaaat
    11881 ctggaatgag gatgcgacct ggcgccaaaa taatttcttc gatcttgtaa acgcattgat
    11941 tatttacggg gagaataatc aactagcctt ggtcaggctt gctcggaccc cagacgaaaa
    12001 ttttgcgaaa tgggatgaac ttctgcagca gcggccagtc gcgggaattt gcgccgcgga
    12061 cacgcacgcg cgcatccgcc tggcatttgg gaaagactgg aaatttcctc aatatgtgcc
    12121 atcgctggaa gttgcccgag agcatgtgct gatttcgccg gatgcgggtg gcggcaatcc
    12181 agcacttgca agcgcctccg aaattgtcga tacactccgg aatggccatt cctattgcgg
    12241 tctggactcg ctctaccctg cggacggctt ttcattccgc atttcgaacg ccaatggttc
    12301 cggcggcccg ggcgacgtat tgcgttcaac gggaagcgcg cgactgcaaa tttcagttcc
    12361 tgccggatca agcctgccgc tgatcaaaat tttccgcgac ggccgtgaaa tagatgagca
    12421 ggagactcgg acaatcgacg aaccagtttc cgggtccggg cgataccgga ccgaagtttt
    12481 cctgcgtcaa cccggattta ccgggtggcg ccgctggact ctctgggtat tcacaaatcc
    12541 aatttacgtt acgactcgct gaagcagacc ttcaataatt aataccattt gcaatttttc
    12601 gatgcgcgaa tccacaagcc ggattttgga tgtgtgcgtc ctggattgct gtatacttcc
    12661 tccaaggtgc ggcgcggctg tttgatccag agcgtggaga ttgcgatgaa taagacgttt
    12721 agctccgttt ttttggcctt cctttttact tttgcgattt ctagttacgc ttctcccggt
    12781 ggcgatatct cgggaactgt aaaagcagag gatggttctc cctttcgagc ggcgttcgtg
    12841 cgcgcgcaaa acgtaaaaac caaaatgatc atgatggtcc tgacaaataa tcaggggcaa
    12901 ttccggacgg ataatcttcc ggcgggggcc tacgaagtgt gggcaacatc caccgggtac
    12961 cggagcgatc cttcccgccg aacggatatc actgtgcagg atggaaagac gacttccgct
    13021 acttttacga tgaagaaagg ttctgtccag tggtcgcagc tgacaaaata tcaagcggga
    13081 atcctgctcc ctgacctgaa gggaaaagat gtggtgctgc aggaatgctt caattgccac
    13141 gcaatgagcc gcatcggcat gatggggcgc gaccgcgaag gctggctgga tgcgattgaa
    13201 cacatgcgcc aagtcggcgt cgctgacatc actccgaaag ttgcggaaca agtttcggaa
    13261 tacttgggaa cggttttcgg gccggacgcc gaaacacctt cctcccccgc acagttgccg
    13321 cagtatcaaa aggtgaagca agagcacgat tatttttcag acgatgcgct gaatattgtt
    13381 tatgtcgact atcaattgac gggagagcct aaggaccggc caggaagcag caagcaggat
    13441 aaagacggaa acatgtggat ggaaatgggc gggggtctct caaagctgaa tccggagaca
    13501 ggcgaacttc acacatggag attaccggac gcaacgcggc cttttattca tgagattttg
    13561 cccacatcgg acggttatgt gtggctcacc atcgaaaagc aaaatgctct ggcaagattc
    13621 gatacgcgaa cggaaaagtt tgaggtgtat cgccagccat acgacggtcc accgcccgct
    13681 gagatggagc cgaatgcgcc gtggccgaat ctgcgctaca ccgcgggagg gcaaaccagc
    13741 gatccacgca ggcacaccgc cgtcgccgac ctgcagggaa atatctggag ttcggggcgg
    13801 ccgctcagca aattcgatat tgaaaccaaa aaatatacat tcttcccgga agttccggat
    13861 acctacggaa tcgctgtcga taaggaagga aatgtctggt tcgccgagtt caattccagg
    13921 gaccatcgca gcattggcaa agtggacgtt aaaacaaaca aggttacaaa atggacgccg
    13981 ccgaattccg aaacgaggcc gcgccgcctg aaaatcgatt cgcagggaat gatctggttc
    14041 ggcgactact tcggcggaaa cctcacgcgg ttcgatccca agacgcagca gtttaaagaa
    14101 tttaaattgc ccggtcctat gccaacgcct tacggaatca gcgtcgatca caatgacgag
    14161 ctttggtacg cgtccatgta tacggatgtg attggcaagc tggatcccag gaccgggaaa
    14221 gtcatcgagt atccgacgcc ctacggtgaa cggggaacga gagatatgtt cgaagatgcg
    14281 cagggacgaa tctggtatgg cgcgcaacct tatggaaaag ttggctatgt ccgcttgcga
    14341 aaagaaacgg aaaaggccgt agcccagaaa taaaatcggg tcacggcctt ttattttttt
    14401 cagtgaagag cgcggacaat tccatctcgg aattctgaaa ttgttttatc cccgctgccc
    14461 ggctactttc cgcttccaca tttcccaatc cttcgggtaa tcgttattgc tggtcagttg
    14521 tgcttcttcc ggagcaaggt acgtcatttt cttgccgaga acttgaactt gaagttccgc
    14581 attcacgcgc atgtacacac tgcgccccac tgcctgggca agacttgccc ctgtaacaac
    14641 cccgccgtgc ccgcgcataa ggaccgctgg cttatccgcc agcttctgga ccaacgcttt
    14701 tgcccgcgcg ggatcggaga tcagcatgtt ggtcatgccc actgcctggc gaatatcgaa
    14761 cagcgggacg cccgcaccca tgtatcctgt catttgatac atcggcataa gtgtttcacc
    14821 tgaaattccc atcagaatta cagcgggagc gtgcgtatgc acaatcgatt tcacatcagg
    14881 gcgcgctctg taaatttccc cgtgaataaa gacttcgctg tacaaacgcg gcttatcgct
    14941 gcccacaggc ttgcaatcaa agtcgacttc caggatgtca gcctctgtta ccatgtccgg
    15001 cgccaaccag cgcgaaatca gaaaatgatt tggattccca ggatgccgca cgctgacgtg
    15061 cccatagcca tccaccacgc cctggtcgta caaaatatga tttgcagtgg caaggtccgc
    15121 gcggagggtg acgacatcct gttgcggatc aggaatcggc ggagtgaagt tttcgctttt
    15181 ggctttcact tccactgcac tcgaagaggc caatactgtt ccggcgagca acaggccgct
    15241 cttggagata aaagcccgtc tggtggtttc agttttcgcc gtcttgattt gtttcatccc
    15301 attctccttt gatttgcctt tttccctacg gagccactgt ggaaaccttt gatctcttga
    15361 gcactgcatc ctgtgtatcg ttcagcatgt gcgcgagtac atcacgagga aggcttttaa
    15421 tcaatggatt tacaatccac gccagcataa tgggaacggt tcgagtcagc gtgatcgatt
    15481 cgttttggac gtagacacct ccgtcttttt cctcaatcct ccagtagctg ttgaggcgcc
    15541 acatgtagcc gtggtcgctg ccttccgcgc gcacgtgttc gtccggctcg cctggattcg
    15601 caacttcggc aatgcgcgtg gagtgagata tgctttcgct gcgtgtgtcg tcgatcgatt
    15661 tcaaggtcac ttcaaaatac ccatccagca caaccgttat aatcgattta ctgaaaaact
    15721 gaagatagac cttcgatgta tttccatttt gctcgatcag tttcgcgcgg cgaatttccg
    15781 gttcatagat gcgcggttca ttcgcataat cgtgcagaac tgcatttaca tccttgagct
    15841 tgcctttggg tatgaaaatc actccagccc aatgatgtat gagcccattc ggaatatgaa
    15901 ttgagttatg accatccacg gtatgcattt cctggatata aatttcaccg cgatggagct
    15961 gttcataggc atgcttacat tgagcttcag gcaatcggtc gatgaccaga aatcgattgc
    16021 cgtccagatc agtctgcatt ttggattcag tgatttctac gtaatggttg aatgcggcaa
    16081 ccgtttccgg cttcagttcc gtagcgcggg cagaaattcc agccagccac ggaaaaattg
    16141 aaagcaggag aacgattttt gggaacaggg ggccaaaacc atttgcgtga tcgcgtgctt
    16201 ttgagcactt caaaagatac cccccgtttt taaaaaaccg aatgggtcca catgtaaagc
    16261 cgttatttga tgactcgccg acagtgcgca tttgtctgga tatatttatc gttgagaatg
    16321 cgcggcaaat taatacagcg aatgatgtaa ttatgttggg acaaaaaagc gtgggcttga
    16381 gtttcatcta aattcaagaa actgttcggt ctttcgaacg atcccacaaa tttggcatct
    16441 ccgcgttgga ataccctgat cggaatgtct tttgtgtccc atccagttca tacatggacc
    16501 tttggacggc tccgatattg gccccataaa cgtgaatgct taccgagggt tgatccgcca
    16561 aggcgtttga aacttgatgg aaatccccga ccgacggaga aacggctgtt acttcaccct
    16621 gagcaagacg ctccggggcc ccatgctgca cgaaacgccc atcgcttacg cgggaataac
    16681 tttgttcaaa ttcagcaccg cgcaggattc ctacaattcc ccatacggta tggtcatgga
    16741 ttggtgattt ctgcgccggc ccccaaacaa aactaactac tgaaaaccgt tcttgggaat
    16801 ccgcgtgcag caaatattgc tggtatccgg attcagaagc ttgcgcgtac cggtcgggaa
    16861 gccagtcgtc tactccaact aattccttga gaagccgcga tccattgcga agaatctcag
    16921 gttcctcatg cgttctttcc atcaaggcgc taaaggaaat aatgaagtcg cgcaggcgct
    16981 cgatgcgcgg tgaaataatt gcgctccgtt ggctcgtttc catttccccg gtcctcaggc
    17041 tccgcagata gaatcaagag tgccttttga agtcgatgga ccttactgta tcatttgcgg
    17101 ctcgcaattt ggagcaagag cggcaatcga agccgtcgta cattgctgcg ttcccggatt
    17161 cccttcccat gcattggatc gcaacgccag tcgccaacta catcctctgc atttaaccaa
    17221 aattaactaa gagtgccgaa atcgcggatc gatagacctg attattggaa aggatcgacc
    17281 aaagaaaatt acgcgtggcc aggctcaatg cgctcgcacg tcttcaagat ttgattcatc
    17341 agaaacccat tgtcagattg acgcgcaacg ttggattcgt ctaacctacc cttccgatgg
    17401 catgcaagtc cgaggggcaa tgaaagtgca tttttttcgc ccgccatcaa aagcattccg
    17461 gcaattggta gcacggataa cgcagcaaag agtccaacgc ctacgatttc tttcgtcccg
    17521 catcgtgaga gatgggtgtg ccggcagaga tttgaaggag acgacagcct aatcgcacca
    17581 tgaccatcga catccatcct cacgtaatca gcaccgatat ccagcgctat tcgaatgctc
    17641 cggtcggcgg ccataaatcc gactggtcgc gagaaagacc cgtaagcgtg gaacagatga
    17701 ttgtcgctat ggatcaagcc gggattgcga aatcggcttt agtgcaggct tccacttgtt
    17761 atggtcacga caactcgtat attgccgacg ctgtagccgc gcacccaact cgtttcaccg
    17821 gcgttttttc cgtggatgtt ctcgcgcctg acgctccaga gaaaattcga tattgggtag
    17881 gccgcaacct gacaggaatg cgtttattca cagcagggag cacaatgccg aatcaggcgg
    17941 actgggtgga tgatccgcga tctttccctg cgtgggagtg cgcgagcgaa ctcggaattc
    18001 ccgtctgcgt gcaaatgacg gtgaaggcga ttcctcagct tgtccgcatg ctggagcgct
    18061 ttccaaaagt tcgcgccatc ctggaccacc tggcaaaacc aacgctttcg gacggacctc
    18121 cttacgccgc tgcggcggct gtttttgagc tggcgaatta caagaactta tatttgaaac
    18181 tgaccccgcg caccgctgcg gaggcacaaa acgggaaagc tactctcgca actttcttcc
    18241 ctttgctcgt atccaaattc ggagcttcgc gaatcgcttg gggatcgaac tatccggcat
    18301 cggaagggac gctctcggaa ttactgacgg tttgccaatc ggcgttgtcc gtgctttcag
    18361 cagaagatca cgattggatc ttcgccaaaa cggcgctgac actttatccc gcgctggctg
    18421 gaaagtagaa ggagtcgatg ctcgaaactc cgcacccgct gacgcttcaa actctgttga
    18481 cgtcacacgc gaataccagc gctttgagaa gcggacagct gcaatcgccg cttgtgaatt
    18541 ttgtttttgc ggatatcaag ccagcgaaca gaggattcaa acctctggtg agggagcaga
    18601 aatttgattt gggcgaactc gccatcgtca cgtttttgca agcgaagaca tacggcaatc
    18661 cgtatgttct gatgcccgcc gtagtcgtcg ctcgcggtca gcatcacact atttttttta
    18721 acccgcagcg cggccatctc aatcctggag atctcaacgg ccgcagagtg ggtgtccggg
    18781 cctacacgca aacgactggc gcgtgggttc gaggtttttt gcaagaagat tatggcgtcg
    18841 acgttcggac gattcagtgg attacgtttg aagatccgca tgttgccgag tacgccgatc
    18901 cgtcgtgggt acagcgggcg ccggaaggca agcaactctt gcaaatgttg atcgatggcg
    18961 aaatcgacgc ggcaattttc ggaagcgaaa atccggaggg cacgccgctc aagccgctga
    19021 ttccgaatgc cgcggaagtt gcagcgcgct gggcccagaa gcacggcggc gccccgatca
    19081 accacatgat ggtaatcaaa gaatcgatta cgcaaactcg cccggacgtg gtgcgcgagg
    19141 tttatcgctt gctgaaagaa agtgcggccg ccgcaccggc aaaagaaacg tccgcgctgc
    19201 gatttggcgt tgagtccgtg cgcaaatcac ttgaaacgat cattcaatat gctgaagggc
    19261 agaatttgat cccgcggcgc ttttcggttg acgaattatt taatgatgtg acgcgctcgc
    19321 tcgactagat gccagcatgc aaccgccagc cacaaagcta gacgaaaccg acgcaagcaa
    19381 agcgcatgtg gttcctcgcg cgcgtgcatg gtacaaacat ctctccatcc aaatcttgtt
    19441 tgcgatgatc ctgggtgtga tcgtcggcca cacttggcct caacacgccg aagcatggaa
    19501 gccgctaggc gatctattca ttaaactcgt gcgcatgctc gttgcgccga tcattttctg
    19561 cacggttgtc cacggaatcg ccagtgttgg cgaagcaaag aaggtcggcc gtatcgccat
    19621 taaatcgcta atttattttg aaatcgtgac gaccatcgcg ctcgtcatcg ccttgcttct
    19681 cgtcaatttc tgggcgcccg gcgccaatat gcatgtggat ttgaagaccc tcgccgatat
    19741 cggcgtgaag gccccggcaa aacccatcac cttcggtcag tttttattga accttgtgcc
    19801 gtctactgcc gtgggggcct ttgccgaggg cgacgttatt cctgtgctgt ttttctcact
    19861 gatgttcgct ttcggattgc tagccctcgg cgcaaaaggc aaacctctca tcgaaacgat
    19921 tcactccgtc acgcaggtct tatttaaaat gattggtttc gcaatgtacc tcgctcccat
    19981 cggcgcgttc ggagcaatcg catttacggt tggcaaattt ggcctggtct ctctcctggc
    20041 tctcggcaaa ttggtcttag agttctacgt atgctgcttg ctttttgtag cgatcgtgct
    20101 cacaccaatc gcatacgtag ccggcgtaaa tattctccgc ttgattcgtt atttcgcagc
    20161 cgaattgatg attgtcgtcg gcaccagttc tggtgaatcc gttttcccca gcgtaatggc
    20221 caagctgcgc ggcctcggca tcgaagaatc caccgtcgca ttagtgctgc caaccggcta
    20281 ctcgttcaac catgacggaa cgtgcctcta ctgggccaca gtctccgtat ttcttgcgca
    20341 agcagtcgga attcatctat cgatcggtca acagttggcg ctactggcga caatgctgtt
    20401 tacttccaag ggcggcgcgg gcgtggccgg ctcagccata gtcattctcg ccaccactct
    20461 ctccgccaca aacgtaattc ccgtcgccag cattggactc atcctcggag ttcatagcct
    20521 tttgtcgtct gctttcgtgc cggtaaacgt attaggcaac tcgctggcaa caatcgtaat
    20581 cgctcgcacc gagcgcgcag ttgatttgca aactctgcaa tcgcaactaa aaagcggccc
    20641 ggaagaaacg acgcaagtga catcagcatt ttagaattcg actagtcgtg aaaaggcttg
    20701 cgttgaaccc catccggacc aaaaccaaac aatgcggatt gcaagccgac ctattcttag
    20761 cccaggtgca gttgggagcg tccgccaagc atcgatttag aaatgttatt gaacagcggg
    20821 gcaagaattg tgactgatcg aagcgttgtt gatgtttccg gggggattgc tccaggctcg
    20881 aagtatcccg gtcgctgcgg tctggatacc agcggggttc caatgggtgt catccaaccg
    20941 gtagttatac tctctttttt gcaaactttc tgctgcctgg gacctcaagg gacccataag
    21001 attgaggacg gggacgcccg atcgacgaag gtcctcttca agcttgtcta gatgggattt
    21061 accttcgggg ggtaattgta acccgtctcg aagcaacgga tagtagacac cgaatttgtc
    21121 aggcaccatt aatacgagaa ggtcgttgcc ggtgcagtgg acgagcgagt taatctccga
    21181 taggtacgtc gggctgtcgt atttcggttg ataaaagttg tccacctcgg ttctcagaaa
    21241 aagcatgcta tccccgttgt gcaagtggcc aaccatgacg aggttctccg atggatttgg
    21301 gaacaaaacc ccattctcta atctccggta accaccactg agaaaaactc tcaaaggtga
    21361 gtaggctagc aggccgtccg cccagacgga aaatctttgc aaaatactac gcctttcgcc
    21421 gaaggccgac ggagcgactc caggattgta tggcccctct gcttgcaacg atgcaggtat
    21481 cttctgacgt tcggacactt gccaaatcac gagaccacca cgcatatgca agcgcttaat
    21541 caactccctg accattttcc agtgaggccc cgcgtttccg ccgttataca cacgcccgtt
    21601 cgaaaggcgg ctcgtcaact gctcaggtag agtatcaccg tccgagttgc tggctccgac
    21661 agcaaatgaa tcaccgacta agatcgcgga agtcatctcg tcagcgtctt cacttggcaa
    21721 atttcgaaaa ccgaatctat ccgtagtaaa gacttctctg tggtattgac ggaaacgaag
    21781 aagatttccc atattcgcaa ggtctccgga agcacgattg ttttcataat ggaaattcgg
    21841 cgcgaatgat cctgccgcgg ttgcatgcag agtcgcggcc tcccacgcgt gaaaataaac
    21901 gcgccccgga ggaacgaatc gaagccctat atccgcgatt aaagttgcaa ggatgagtac
    21961 tgggatcaaa aatcgaagtc ccaacaattt ttgtcctggt ccgcaacggt tttgctggga
    22021 agtttccatg cgcgtcaaaa ttggaagtac agaaatgggg tagccttgcc cccatagata
    22081 gcgacgagac atgcggcgaa cagtgctgtt aacgcgagag ccgaagccca gttccagcgg
    22141 tgagataact caaaagaatt cgggcagcaa tgtgccagac caccggctat caaaatcggg
    22201 agcaccaaag tccatgctcc caaatcgccc gactccgggt tccacacaaa catttttctc
    22261 aaccatccgg ccgccatcgt gaaactcgtc gcacgaaata acacccaccc aattacaaca
    22321 aggaagaacg tgaacattct gcgcagccac gccggcacaa agacaattcg acggaaactg
    22381 taacg
//
LOCUS       DASZMB010000166        15014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3720510, whole genome shotgun sequence.
ACCESSION   DASZMB010000166 DASZMB010000000
VERSION     DASZMB010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 15014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15014
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3720510"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1400
                     /locus_tag="VG272_07545"
     CDS             138..1400
                     /locus_tag="VG272_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWF13574.1"
                     /translation="MRRLSNGIIGIIFALVVCGPTGFAAQQQTPQQPQTQTQQQPTAS
                     DDSKQPQAQDQSAPSSSDDDATMKGVKPGSDKDVNSIGNRGVGNGVNLYSLQREIALG
                     KQAAMEVEKTARMINDPIVTEYVNRVGQNLVRNSDAKVPFTIKVIDSDEINAFALPGG
                     FFYVNSGLILRADEEAELAGVMGHEIAHVAARHGTKTATKGEIMQWATIPLILLGPGG
                     WAGYGLYEGLNLAIPMSYLKFTRDAEREADFLGLQYMYKAGYDPNAFVSFFEKIEAEE
                     RRRPGSIPKVFSTHPPTPDRVQKAQEEIATILPARDEYVVTTSEFDTVKARLRRIENK
                     NKLDDKKNSADKPTLRTRTEQTPQDKQDKTGSQSPQSGGDDSDPDRPVLKKRTDDGSG
                     SGSGSGSGSGSGSGSSTGTGSGSGSGTN"
     gene            complement(1563..2174)
                     /locus_tag="VG272_07550"
                     /pseudo
     CDS             complement(1563..2174)
                     /locus_tag="VG272_07550"
                     /inference="COORDINATES: protein motif:HMM:NF016271.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF507 family protein"
     gene            2526..3353
                     /locus_tag="VG272_07555"
     CDS             2526..3353
                     /locus_tag="VG272_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWF13575.1"
                     /translation="MLTRPIPSTGEQLPVIGIGTYKGFDVGSRRDARAKVGEVLRTLY
                     SHGGSVVDSSPMYGRAEEVAGDLITEQNAREKSFIATKVWTEGRSAGIGQMKRSMKLL
                     RVDQIDLMQIHNLVDWRTHLATLRTWKEEGRIRYLGVTHYAASACADLEKIMRAEPLD
                     FVQLNYSLDERQADQRLLPLAEERGIAVLANRPFGQGSLHKALHGKPLPPWARESGCE
                     TWPEILLKFTLAQKAVTCAIPGTGNPQHMAENCRAGDGPLLDASLVKKISRLLGFGA"
     gene            complement(3443..4561)
                     /locus_tag="VG272_07560"
     CDS             complement(3443..4561)
                     /locus_tag="VG272_07560"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF13576.1"
                     /translation="MRNRRDFIKSVVGASAGVVFTSCSICDAMAAGRLAQAGSGVKHK
                     PVMVGNRRVKTVDIHCHVSVPEVTDLLKGTKIERRLGAGLAQGGYDDAGLTPARIQTM
                     DEMGIDVQAVSINSFWYSADRELVTKLIDLQNQKLSELCGTHPDRFVALASVALQFPE
                     LAAQQLEVAMKKYGLRGVGIGGNCEGKELSSPEFDPFWAKAEELQALIFIHPQDSANA
                     TGVRDRVQGNGILSNVIGNPLETTICLSHLILEGTLDKFPNLKICAAHGGGFLPSYDG
                     RIDQGCRVFPKTCNKTIKMKPSEYLKKIYVDTIVFNQEALRHLIAVVGAGQIGLGTDY
                     PFPWDFAPVDYVMSQPGLSDADRIAILGGTMSKLLKIS"
     gene            4998..6038
                     /locus_tag="VG272_07565"
     CDS             4998..6038
                     /locus_tag="VG272_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWF13577.1"
                     /translation="MSLPTRPLGSSGLQITPIGFGAWAIGGGGWAFSWGPQDDNESIA
                     AMRHALELGVNWIDTAAVYGLGHSEEVVGKLLRDLPSSQRPLIFTKCGLTWDENNPMQ
                     QAKRILKPETIRRECEASLRQLGVERIDLYQFHWPDEIGTPIEDSWGEMVKLVEQGKV
                     RAAGVSNFTVDLLEKCEAIRHVDSLQPLFSMISRATAEQLLPWCKNHNTGSICYSPMQ
                     SGILTNGFTTERVSKMASDDWRRRGGEFQEPKLSRNLALRDALKPIAARHDVSVSAVA
                     VAWTLCSPGLTGAIVGTRTPAQVDGWIKAATLQLTQEDLTEIAEVIARTQAGSGPAQP
                     PLTAASKSGSSL"
     gene            complement(6213..10343)
                     /locus_tag="VG272_07570"
     CDS             complement(6213..10343)
                     /locus_tag="VG272_07570"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HWF13578.1"
                     /translation="MKMRTVRSLLLAGLFLAMGIMLMPSAQAQQTLGGITGTVTDTSG
                     AVISGATITLVGNETKLNRTQTSSTTGSYLFVNLPIGTYTLTFTQQGFQTQNIPSIAV
                     QANRTATVNAELKIGNVSESITVEETPLLNMVDTTNGYVMDKLQIDAVPLPTGSFTGL
                     AILSPGVNAELSAGTGANAGLGNAPVWANGQRDTSNTFLLNGVDARSLFNGKSTSQVS
                     SARVVNNTGVSGASSLSAIPIQSSASVYLAIGESIPSPAPETIQEVRVNTSMYDAQQG
                     STSGAHIDMSTASGTNNIHGILYGHFGNNALNADPFFYNADPNIPANEKNPGLHRYTA
                     GGAIGVPIKKDKLFFYGSYQHTRASDDEIGISRAFVPPDFGQALSGVSPTGVPYNPGC
                     SASNPRSAACLANVAILDTTFASPLNTSFAGPVMTTPNFTGVPCGSGNSDTCGINPIA
                     YTLFNYKLPNGQYLIPTANPNSVIRNSGNSALITAFPEDAEIPGTAVFKADQAVANLD
                     WNPNSTHSFSAKYYFQHDPTVAPFAYSMFAGFAQHLDAGSHVIALSHTQVVKSNLSVT
                     ETFGFIREKAYSTEDQPLTAAQLSSACETLTAQSPTDCTMNTLGTTFFPGISMVFAGS
                     TATPYPYSMNIGAGAASQGAFTGVFQNRFNPSANAIWTLGRHTITFGGSYAYTQLNTK
                     DQRNQIGMIASQDFTNFITGNIIPNYDYNISALLVGNPNRYWRANETGEYFQDKFQWK
                     SNIAITAGVRFDWDGGLTEKNGNLLNFDPSKYSYDPTTDAYAGNGLIVAGNNPKAGTP
                     GVSKTTLTGRQWAIDPRLGIAWSPKMFNSKVVVRAGWGMYYDRGELFAYLSPGLSQNI
                     TGGGPFGVNQQQPFVATQSCNIPADNCSATLEAPWGLTPVQPPGNATGIVLPNACALA
                     NNNSTNPFAPPTYSCTANTANPTPFYLGAYARNNKLPYTMNSTLDIQWQPRNDLAIDI
                     GYVNALGRHEIIPVPFNQSRIASPSNPLCGVAATCANPSGSPFAQSYTYGMTLQNNAA
                     DCNIFGNFCPANLPNNQPYLTNPEGGNVDLRVPYIGFGAESELYTAEGISAYNALQAH
                     VEKRLSHGLQLGVSYTYSRSFDEQSALGLFYNGNNPLNLKDGYGPSDFDRTHVINIDY
                     HYELPKFYNANSWEGKVVDGWAVQGVVTIQSGQPYSVIDYSGAVGSAFYSIFDGITNP
                     IDPLAPGCTAKSALTGAVGVTPGSPALKADCFALPLLAPGALNGAIPSNDPFETNFTT
                     GQRNIFRQSWQRRADISILKSTQITERYTLKYTFDVFNLTNTPSFDIPIDNVTQNLNF
                     SAYPISGLCTSPAQTTGCSNASNFGNPFFNAPGGLGQVTKTIGSARQVQMSLSLTF"
     gene            complement(10609..12000)
                     /locus_tag="VG272_07575"
     CDS             complement(10609..12000)
                     /locus_tag="VG272_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWF13579.1"
                     /translation="MATNRTESVPVKTGDYGLRGEALSPMETLAQSVSTIAPTSTPAA
                     TIPLVAALAGNGTWLAYLLAMSAILLVAWCISRFARYSASPGSLYTYAAMILPPWLSA
                     IVAWSLLLAYIATGSSVIGGFYHFSNILLTDFLGHGVSGAALALVVTGASVWIAWRDV
                     KISARMMLWIEALSLLLIVVVVVLVLFRHGTRLDTSQVHLTAMTGSGLRLGLVLALFS
                     FVGFESATTLGAEARNPLTTIPRVVIESAVIAGLFFIVSAYAEVLGAHVAGQDLGTSD
                     APMHILANVAGIPVLGLLIDIGAAVSLFAGTLGCITAAARVLLLMSHHGLAHGAFRTT
                     HVRNETPTFALIATGIAAVLPVAILAFYGSSGLDVYGWMGSLATYGFIVAYALVCISL
                     PRYLRDHGVFRQGAQIIPWLAFVAMLAAMAGNLYPVPEGPYGKLPYIYLAYLSAGMLW
                     FFWQARKEKTGNA"
     gene            12254..13549
                     /locus_tag="VG272_07580"
     CDS             12254..13549
                     /locus_tag="VG272_07580"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HWF13580.1"
                     /translation="MLERIFHLAENQTTVRRELLGGLTTFMAMAYVVVVNPRILGEAG
                     MPVEGVLFATCISAAIATLVMGLWANYPIALAPGMSLNAYFTYSIVLGRGVPWQTALG
                     VVFLSGSLFLLLTLTNIREHIVNDIPDSLKHGTAAGIGFFIAFVGLRNAKIIVASAAT
                     FVTLGKSSDPQVRLAALGLIIIAILMSRKVGGAILIGIVAIALAGIPLGLTTVPSHIF
                     SWPHPGGTFLKLDFRSAFKIGLGDLVFVFFFVDLFDNVGTLVGVCEQGGFLRDGKFPR
                     ASRALLADAFGTMAGALTGTSTVTSYIESAAGVAAGARTGLGNMLIAALFVAAIFCAP
                     LVAAVPTYATAPALILVGALMCASLAKVDWDDFTEAFPAFITTVATPLTFSIATGLSL
                     GLLSFTFIKIGTGKIKQISPLIWVLSVLFLLRFAFLGAE"
     gene            14032..>15014
                     /locus_tag="VG272_07585"
     CDS             14032..>15014
                     /locus_tag="VG272_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13581.1"
                     /translation="MPTQIVEDEAVPQSITTEIPAFGRKAIQYFISFFLCFIVLAVFI
                     PLSPLMPAQGLDTSWMFAMNQGVAHGFVFGKDIVFTFGPYSSIYTELYDPATDKLMVF
                     GSFFLGICYCFQLLLLAKHKSNYGLLFYGIFLACLMDSRDALLFSYPLILSLVVYRVT
                     LPHEHAWKLHLENSLSYGVALLFAPLGLLPLIKGSLLPMCGMTAFLCFAIFWLNDKKI
                     LAWATVGIPAISCALFWRISGQSLFALPGFFWNMRQIISGYNEAMSFPGNSGECVLYI
                     LAAVLIIFAIASTIRAPRISTSFLSISYAFFLFLAFKSGFIRHDPWHNIIAG"
BASE COUNT         3473 a         4014 c         4017 g         3510 t
ORIGIN      
        1 aattgcttga acgggcaggt tttccacgcc agacggggaa acctatttac tcgcgcccac
       61 atctcatcgc gcagggtgta ctcttgaggg ataagcgcgt aatgtatact acaggtgttg
      121 gacctacagg aggagccatg agacgcctct cgaatggcat catcggaatt atctttgcgc
      181 tggtggtttg cgggccgacc ggcttcgccg cgcaacaaca aacgccgcaa caaccgcaga
      241 cgcagactca acagcagcct actgcttcgg acgatagcaa acaaccgcag gcgcaggacc
      301 agagcgctcc ctccagttcc gatgacgacg ccaccatgaa gggcgttaag cccggctccg
      361 ataaggacgt gaattccatt ggcaaccggg gcgtgggcaa tggcgtgaac ctctattccc
      421 tgcagcgcga aatcgcgctc gggaaacagg cggccatgga agtcgaaaag accgcccgca
      481 tgattaacga cccgatcgtc accgagtacg taaatcgcgt gggccaaaac ctggtgcgca
      541 attccgacgc gaaagttcct ttcacaatta aagtgatcga ctcggacgaa attaacgcgt
      601 tcgccctgcc tggcggattc ttctacgtga atagcggctt gattctgcgc gccgatgaag
      661 aggctgagct ggcaggcgtc atgggccacg agatcgccca cgtcgccgca cgccacggaa
      721 cgaaaacggc caccaagggc gagatcatgc agtgggctac gattccgctg atcctcctgg
      781 ggccgggagg ctgggccgga tacggtttgt acgaaggctt gaacctcgcg atcccgatga
      841 gctacttgaa atttacgcgc gatgcggagc gcgaagcgga ttttcttggc ctccagtaca
      901 tgtacaaagc cggttatgat ccgaatgcct tcgtcagctt ctttgaaaaa attgaggcgg
      961 aggaacggcg gcgtcccggc agcatcccaa aagttttctc gactcacccg cccacaccgg
     1021 accgcgtgca aaaggcgcag gaagaaatcg ccaccattct tccggcgcgt gacgaatacg
     1081 tagtgacaac gtcggaattc gatacggtta aggctcggct gcggagaatc gaaaacaaga
     1141 acaagttgga cgataagaaa aactccgcgg acaaacctac tctgcgaact cggaccgaac
     1201 aaacacctca ggataagcaa gataaaactg gttcgcagtc cccacaatcc ggaggagatg
     1261 attccgatcc ggatcgcccg gtgctgaaga agcgcacgga tgatggcagc ggcagcggat
     1321 ccgggagcgg ctctggcagt ggatcaggtt ctggttccag cacgggcaca gggtccggct
     1381 ctggatcggg caccaattaa tcctcaattt atttcttaaa atgaaaaaaa cgtccggcat
     1441 gccggacgtt ttttccccgt gtagcgcccg ccttcaggcg ggcaccaagc aacagtacaa
     1501 aaccaatgct cgcctgaagg cgagcgctac aaaaaacttg aatcaaatgt atggctgcgg
     1561 ggttaggaat tactcggcag gtgaacgatt cccatgcggc gcatttcatc cgcgtaatat
     1621 ttgcggtaaa gcacgtccca ctcttcactt gcttccaata tttctttctt ctgcgagctg
     1681 atctttttgc ggacttctgc gtccaccgcc tcttcttcct tcaataaatc ctgaagaatt
     1741 tgcagggttt tctggcgaat ggtgtctcgg tcttcgatga attcaacgtc gtcgcaagca
     1801 accagaatgt cggtgatcac atgggaaagg cgaattgatt tttcgcgggt gagtcgcatc
     1861 agtgggttat tgaatttccc tttcctcgtg gatcaacgca agatcagctt tctttgccgg
     1921 gccagttcgc ccttcacttt tttgtacatt tcctggtact ggacgccggt gcgacgcatt
     1981 tcttcggcat gcttggtgag aatttcgcga acttcttcgt tcaggcggtc ttcgacgctg
     2041 atctcttcct gcatagcaac tcgcacgcgt tgtgtgacgg aagccgcatc cggcgcttca
     2101 attactttgg ccgcaagcag gcgcttcacc acttcccctg ccatgtatcc gacgtaatcg
     2161 cgcgaaagca gcatcgttta tgtcctctga agaattcgga gaagaagttt actgtcccat
     2221 gagaagaagt gtcaaggaag gttcgccggc gatggcgggg gatttgagtt gcacaaacag
     2281 aaaagcgccc gcccaaaagg cggctggccg ctacaaaacc aaattcgagg tcccgcggtt
     2341 ttcgaagcgg cctctccgtc ccatgctctt cgggacggtt caccgtgagc gttcaccgtg
     2401 aatggagagg gcaggttagg ccttagaccg gaccttttcc ggttaactcg tttacgtcgt
     2461 gcattttgca atcgctgcta attgtaaaaa atatcggacc tgcctatcct atattgtctg
     2521 ttcctatgct cactcgacca atcccatcca ccggcgaaca actccccgtc atcggcatcg
     2581 gcacctacaa aggttttgat gtcggctcca ggcgcgacgc acgcgccaaa gtcggcgaag
     2641 tcctgcggac tctttactcg cacggcggca gcgtcgtaga cagttctccc atgtacggcc
     2701 gggccgagga agtcgcggga gatttgatta cggagcaaaa cgcacgcgaa aaaagtttta
     2761 ttgccacgaa agtttggacg gaaggccgca gcgcaggcat cggtcagatg aagcggtcaa
     2821 tgaaattatt gcgggtcgac cagatcgact taatgcaaat ccataatctg gtcgattggc
     2881 gaacgcatct ggcaacgctt cgcacgtgga aagaagaggg ccgcatccgc tatctgggcg
     2941 tcacgcacta cgccgcgagc gcctgtgcag acctggagaa aatcatgcgc gccgaaccgc
     3001 tcgatttcgt gcagctgaat tattcgctgg acgaacgcca agcggatcaa cgacttttac
     3061 ctctcgccga ggaacgtgga atcgcggttc tcgcgaatcg cccatttggc cagggaagct
     3121 tgcataaagc cctccacgga aaaccgcttc cgccgtgggc gagagagagc gggtgcgaaa
     3181 cgtggcctga aatcctgttg aaattcacgc tggctcaaaa agcagtcacg tgcgcgatcc
     3241 ccggaactgg aaatcctcag cacatggcgg aaaattgccg ggcaggcgat ggccccctac
     3301 tggatgcatc tttagttaaa aaaattagta gattattggg attcggggct taagtaggac
     3361 aggcttcagc ctgtcttctt ttgacttttg ctgtaaaaga gaaagtcaaa accggacagg
     3421 ctgaagccta tcctacttga atctacgata ttttcagcaa cttgctcatc gttccaccaa
     3481 ggatggcgat ccgatccgcg tcgctcaacc cgggctgcga catcacataa tcgacgggcg
     3541 caaaatccca cggaaacgga tagtccgtcc ccaagccgat ctgccccgcg ccgaccacgg
     3601 cgatcaagtg ccgcaacgct tcctgattga aaacaatcgt atccacgtaa attttcttca
     3661 gatattccga cggcttcatc ttgatggtct tgttgcacgt ttttggaaat acacggcatc
     3721 cctgatcgat tcgtccgtca tagctgggca aaaatccgcc gccatgcgcc gcgcaaattt
     3781 tcagattcgg aaatttgtcg agcgttcctt ccagaatcag atgggaaaga cagatcgtgg
     3841 tttccagcgg attcccgatg acattgctca aaatgccatt tccttgcacg cggtcgcgaa
     3901 cgcccgtagc gttggcgcta tcctgcggat gaatgaagat gagcgcttgc agttcctcgg
     3961 ccttggccca gaaaggatcg aattcagggg acgacaattc cttgccctcg cagttcccgc
     4021 caatgccgac gccgcgcagc ccatattttt tcatggccac ttcgagctgt tgcgcggcca
     4081 actctggaaa ttgcaacgcc accgaagcca aggcgacaaa acggtccgga tgcgtgccgc
     4141 acaattctga taatttctga ttttgcaagt cgatcagctt ggttacgagt tccctgtccg
     4201 cagaatacca gaaggagtta atgctgacag cctggacgtc gatgcccatt tcgtccatgg
     4261 tttgaattcg cgcgggagtc agccccgcgt catcgtatcc accctgcgca agtccagcgc
     4321 caaggcgacg ttcaattttc gtgcccttca aaaggtcggt aacctccggc acgctgacgt
     4381 ggcaatgaat atccacggtt ttcacacggc gattccctac catcaccggt ttgtgcttca
     4441 caccgctgcc agcctgcgcc aaccgccccg cagccatcgc atcgcaaatc gagcagctag
     4501 taaacacaac tccagcggac gcgcccacaa cgctcttgat gaaatccctg cgatttcgca
     4561 tattttgttt ctccttacgc agcccgcaaa atttagcgat gctctttccg ctttcttcgc
     4621 gtttctcagc gccctctgtg ttaaatcttt tcaaatctca acacagagca cacaaagaaa
     4681 aacggaggcc cacaaagtga actggaatcg gatcgattct gaccggaagt ctatcggcac
     4741 ccgcaccgca tgtcgataac aaaatgagag aacaactttt gcggaacgat tgaccgctga
     4801 cgattgaaat gctcgttgcc ttacgtagtg gccggccgat ttttgggcgg gcactctgcg
     4861 caaatggcag gattgcccgg cccaccgcgc ctcacccgtt ccgctggaac acgcaggaaa
     4921 tcgcgtttac actggaagcg agaaattcac gcagggtttc tgatattttt atttttgcaa
     4981 aatctggagg cgcaaacatg agcttgccga cacgtccact aggttccagc ggtttgcaaa
     5041 tcactcccat aggttttggc gcatgggcca tcggcggcgg aggatgggcc ttcagctggg
     5101 ggccgcagga cgataatgaa tccatcgcgg cgatgcgcca tgcgctggaa ttgggcgtca
     5161 actggattga taccgccgcc gtttatgggc tgggccactc ggaagaagtt gtcggaaaac
     5221 ttttgcgcga tttgccttcc tcgcagcgcc cgctcatctt caccaagtgc gggctcacct
     5281 gggacgaaaa taatcccatg cagcaggcca aacgcattct caagcccgaa acgatacgcc
     5341 gcgaatgcga agcatcgctg cgacagctgg gcgttgagcg catcgatctc taccaatttc
     5401 actggccgga cgaaatcggc acgccaattg aagattcctg gggcgagatg gtgaagctcg
     5461 tggagcaagg aaaagtgcgc gccgccggcg tatcgaattt cacagtggat ttgcttgaaa
     5521 aatgcgaagc aattcgtcat gtcgattccc tgcaaccgct tttctccatg atcagccgcg
     5581 ccactgcgga acaattgctg ccgtggtgca aaaatcacaa cactggctcg atctgctata
     5641 gccccatgca atccggaatt ttgacgaacg gatttacgac ggagcgcgtc tcgaaaatgg
     5701 cgagcgatga ttggcgtcgt cgcggcggcg aattccagga accgaaactg agccgcaacc
     5761 tcgcgctgcg cgatgcgctg aagccgatcg ccgcacgcca cgacgtttcc gtctccgcag
     5821 ttgcggtggc gtggacgctt tgctcgccgg gcttgaccgg cgccatcgtc ggcacacgca
     5881 caccggcgca agtcgacggg tggatcaaag ccgccacctt gcagctcaca caggaagacc
     5941 tcacggaaat tgctgaagtg atcgcccgaa cgcaggctgg ttcgggtccg gctcaaccac
     6001 cgctaaccgc tgcatcgaaa agcggaagct ctttataaat ttcgtctata gataggttcc
     6061 cgtggcatag gcttcagtct gtggaagttg ggccttgcaa ggactaaacc ccacaggctg
     6121 aagcttttgc cacgggaacc cataccgtta aaaataaagc ggcggcatcc agtttggatg
     6181 ccgccgcttc tgattttaga cgccagccta aattagaacg tcaggctgag cgacatttga
     6241 acttgccgcg cactgccaat cgtctttgta acttggccga gaccacccgg tgcgttgaag
     6301 aaagggttgc caaaattgct ggcgttggag cagccagtgg tttgggctgg agatgtgcat
     6361 aatcctgaaa ttggataggc gctgaagttc aggttttgcg ttacgttgtc aatcgggatg
     6421 tcaaagctcg gtgtgttcgt caggttgaaa acgtcaaaag tatatttcaa cgtataacgc
     6481 tcggtgattt gcgttgactt caaaatagaa atgtcagcgc gacgttgcca cgactggcgg
     6541 aaaatgttcc gctgccccgt agtgaaattc gtttcgaagg gatcattgct cggaatcgcg
     6601 ccattcaatg cgccgggtgc gagcaacggc aacgcgaagc aatctgcctt cagtgccgga
     6661 gatccgggtg tgacaccgac cgccccggtc aacgcgctct tcgccgtgca gcctggcgcg
     6721 agcggatcga ttgggttcgt aattccatcg aagatgctat agaacgcgct cccgacggcg
     6781 cctgagtaat caattacgct atagggttgc ccgctctgaa tggttacaac tccctggact
     6841 gcccatccat ctaccacttt gccttcccag gaattggcgt tatagaactt aggaagttcg
     6901 taatggtaat cgatattgat gacgtgtgtc cggtcaaagt cggaaggacc atatccgtcc
     6961 ttcaagttga gcggattgtt tccgttgtag aacagaccga gggcgctctg ctcgtcaaac
     7021 gagcgcgagt acgtgtaaga gacgccgagc tgaagtccat ggctgagcct cttctcaacg
     7081 tgcgcttgca gggcattgta ggccgagatg ccttcagctg tgtaaagctc ggactcagca
     7141 ccgaacccga tatagggaac gcgcaagtcc acgttgccgc cttccgggtt tgtcaaataa
     7201 ggctgattgt tgggcaaatt cgcggggcag aagtttccga aaatattgca gtccgccgca
     7261 ttattttgga gcgtcattcc atatgtgtag gactgggcaa atggcgatcc cgatggattc
     7321 gcacatgttg cagcgacacc gcacagcggg ttcgatggag atgcaattct cgactggttg
     7381 aacggcactg gaataatttc atgccggccc agagcgttta catacccaat gtcgatggcc
     7441 aggtcatttc gtggctgcca ctggatatcc agcgtgctgt tcatcgtata ggggagcttg
     7501 ttattccgcg cataggctcc caaataaaac ggcgttgggt tcgccgtgtt tgcagtgcag
     7561 gaatacgttg gaggagcaaa tgggtttgtc gaattgttgt tagccaatgc gcatgcattc
     7621 ggcagaacga tgccggtcgc attgccggga ggctgcacgg gagtcagccc ccaaggcgct
     7681 tctagcgtgg ctgagcagtt gtctgcggga atgttgcaag actgcgtggc tacgaagggc
     7741 tgctgttggt taacgccgaa cgggccgcca ccggtgatgt tctgagaaag gccaggagac
     7801 aggtaagcaa aaagttcgcc gcggtcgtag tacatgcccc aaccggcgcg gactacaact
     7861 ttgctgttaa acatcttggg gctccacgca attccaaggc gtggatcgat tgcccattgc
     7921 cgccccgtca gtgtcgtctt acttacacct ggagttcccg ctttcgggtt gttccctgcg
     7981 acgatcaatc cgttcccggc ataagcgtct gtcgtcgggt cataggagta tttcgaagga
     8041 tcgaaattga ggaggttgcc gttcttttcc gtcagtccgc catcccaatc gaagcgaacg
     8101 cctgcagtga tggcaatgtt gctcttccac tgaaatttat cctggaaata ttcgccggtt
     8161 tcattcgcgc gccagtaacg gttaggattt ccaaccagca aagcactgat gttgtagtca
     8221 tagttgggaa tgatgttacc ggtaataaaa tttgtaaaat cctgcgaagc aatcataccg
     8281 atctggtttc gctgatcttt cgtgttgagc tgtgtatagg cgtagctgcc accgaacgtg
     8341 atggtatgcc ggcccagcgt ccagatggca ttcgctgacg ggttgaacct gttctgaaaa
     8401 actccggtaa acgcgccttg cgaagcggcg cccgcaccga tgttcatcga atagggatac
     8461 ggggtggcgg tagaccccgc gaataccatg ctgattccgg ggaaaaatgt agtgcccaga
     8521 gtgttcattg tgcaatccgt aggcgattgc gccgtaaggg tttcgcatgc tgacgacaat
     8581 tgggcggccg taagcggctg gtcctcagta ctgtaggctt tctctcgaat aaaaccgaac
     8641 gtttctgtaa cgcttaggtt ggatttcaca acctgcgtat ggctcaaggc gatcacatgg
     8701 cttccggcgt ccaagtgctg tgcgaatcca gcgaacatcg agtacgcgaa gggcgcaacc
     8761 gtaggatcgt gctgaaagta gtacttcgct gaaaaggagt gcgttgaatt ggggttccaa
     8821 tccaggttcg ctactgcttg gtccgccttg aataccgcgg tcccgggaat ttcagcatct
     8881 tccggaaacg cagtaatcag tgcgctattg ccactattcc ttataaccga gttcgggttg
     8941 gcagtgggaa tcaaatattg gccgttcggc agcttgtaat taaagagagt gtaagcaatt
     9001 ggattgatcc cacaagtatc agaattgcct gaaccgcaag gaacgccggt aaaatttggg
     9061 gtcgtcatca ccggaccagc gaaactcgta tttagcggcg atgcgaaggt agtatccaaa
     9121 atggcgacat tggccaaaca tgcagctgac cgagggttgg atgcggaaca accgggattg
     9181 tatgggactc ccgttggcga cacaccagac agcgcttggc caaaatccgg cggcacgaac
     9241 gcgcgggaaa taccgatttc atcgtccgat gcgcgcgtgt gctggtagct gccgtagaag
     9301 aacagcttgt ccttcttgat gggaacccca atagctccac cagcggtgta gcggtgcaat
     9361 ccgggattct tttcgttggc gggaatgttc ggatcggcgt tgtagaaaaa cgggtcggcg
     9421 ttcagagcgt tgtttccgaa atggccgtag agaattccat gaatattgtt ggtgccggaa
     9481 gcggtgctca tgtcaatgtg cgcgccgctt gttgagccct gctgcgcgtc atacatcgaa
     9541 gtgttcacgc gaacttcctg aatggtttca ggagcgggcg aaggaatgga ttcgccgatc
     9601 gcaagataga cggacgcgct gctctgaatg ggaatcgcgc ttaagctgga agcgccactg
     9661 acgccggtgt tgttcacgac gcgcgcggaa gatacttggc tggtgctttt gccattaaag
     9721 aggctacgtg catccacgcc attcaagagg aacgtgttgc tggtgtcgcg ctggccgttg
     9781 gcccagactg gcgcgttgcc taaacccgcg ttcgctccgg ttccggccga aagttccgcg
     9841 ttcacgccgg gcgacaaaat cgcgagaccg gtaaacgaac cggttggcag aggaacggca
     9901 tcgatctgca gtttatccat cacgtacccg ttcgtggtgt ccaccatgtt caagagcggc
     9961 gtctcttcca cggtaatgga ttcacttacg tttccgattt tcagttcggc attcacggtc
    10021 gccgtgcgat tcgcttgcac agcgatggag gggatgttct gcgtttgaaa accttgctgg
    10081 gtgaacgtaa gcgtataagt cccgatgggc aggttcacaa acaggtaaga gcctgttgtg
    10141 ctggaggttt gcgtacgatt gagtttggtt tcgttgccga cgagagtaat agttgcccct
    10201 gagatgaccg caccggatgt atcggtgacg gttccagtaa ttcctccgag cgtctgctgg
    10261 gcttgcgcag acggcatcaa cataatcccc attgcgagga ataaacctgc aagcaataga
    10321 gaacgaacag tgcgcatttt catacttaat cccctctcca aagtcaccaa cagaaattat
    10381 ttaaaaaata attttcgagg aactttaaaa cgaacacttc ccccttgtta ccactacaaa
    10441 cactcaacaa taattgaatg gaacttacgc taatgcagcc cgcttgaaaa gcgcaaactt
    10501 tcaatcttcg gtatcactta tttgtatctg gtatttgttt tcgcctgggc ctgtaactca
    10561 gagcaaacat tgcagcggga taatctgacg gcagaatata tcgtggggct aggcgtttcc
    10621 agttttttct tttcgcgcct gccagaaaaa ccagagcatc ccggcggaca ggtaggccag
    10681 gtaaatgtaa ggtaattttc cgtagggtcc ttccggcacc ggataaagat tcccagccat
    10741 ggcggcaagc atggcaacaa atgccagcca cggaatgatt tgcgcgccct ggcgaaagac
    10801 gccgtgatcg cgcaaatatc tcggaagcga aatgcagacg agcgcatacg ccacaatgaa
    10861 accgtaggtt gcgagggaac ccatccagcc gtacacatcc aagccgctcg atccgtaaaa
    10921 ggccagaatc gcaaccggca ggactgccgc aattcctgta gcaatgagcg cgaaggtagg
    10981 cgtctcgttg cggacatgcg ttgtgcgaaa agcgccgtgc gcaagtccgt gatgcgacat
    11041 gagaagaaga acgcgcgccg cagccgtgat gcaaccaaga gtgccggcaa acaagctgac
    11101 cgccgcgccg atatcgatta ataatcccag cactggaatg cccgcgacgt tcgccaaaat
    11161 atgcatgggc gcgtcgctcg tgccgaggtc ttgccccgcg acgtgtgcgc ccaaaacttc
    11221 cgcgtacgcc gatacgataa aaaataatcc tgcgatcaca gcactctcaa tgaccacgcg
    11281 aggaattgtt gtgagaggat tgcgagcttc ggcaccaagc gttgttgcgc tctcaaaccc
    11341 gacgaaactg aaaagcgcga gaaccaaacc taatcggagg ccgctgcccg tcatggccgt
    11401 taaatgcacc tgacttgtat ccagacgcgt cccatggcgg aacagaacga gcaccaccac
    11461 gacaacaatc aacagcagag agagcgcttc gatccacaac atcatgcgcg cagaaatttt
    11521 tacgtcgcgc cacgcgatcc acacggaggc gcctgtaacg accagcgcga gggctgcacc
    11581 tgaaacgccg tgtcccagga aatcggtcag caaaatattc gaaaaatgat aaaagccgcc
    11641 aatcacgctc gagcccgtcg cgatgtacgc aaggagcagg ctccaggcga cgatggcgct
    11701 cagccacggc ggcaaaatca tcgccgcata cgtatagagc gaaccgggcg acgcggagta
    11761 tcgcgcaaaa cggctgatgc accacgccac caataaaatt gcgctcatcg ccagaagata
    11821 tgcgagccag gttccgttcc cggccagcgc tgccaccagt ggaatcgtgg ccgctggagt
    11881 ggaggtgggc gcaatcgtgg aaacggactg cgcgagcgtt tccatcggcg aaagcgcttc
    11941 gccgcgcaat ccataatctc cagtctttac gggaaccgat tctgtacggt tagtggccat
    12001 caggtctcat gtttctcggc gtgcgcttag cccggtcgga catgccgtac catacctgtc
    12061 cgcctgggtt ggtgcaaggt tctacaggtg ttttgacgat ttgaaaaacg aatcggatgg
    12121 cgcagcaagt gcgattcgat attcgtcatg ggaaaagtta ccctgtgtgt ccggttttta
    12181 tttaccacag gttcttcgtt gacgcagcgg ggcgccccgg cctatagtgg cgcacttttc
    12241 aaagaggaaa gcattgctcg agcgaatttt tcatctcgcg gaaaatcaga ccacggtgcg
    12301 ccgcgaactt cttggcggcc tcactacctt catggcaatg gcctacgtgg tagtggtgaa
    12361 cccgcgtatc ctcggcgaag cgggcatgcc agtggaaggc gtcctgtttg ccacttgcat
    12421 ttcggcggca atcgccactc tcgtgatggg cctgtgggct aattatccga tcgcgctcgc
    12481 gcccggaatg tcgctgaatg cgtacttcac gtattcgatt gttctgggcc gcggcgtgcc
    12541 atggcaaact gcgctcggcg ttgtatttct ttccggttcg ctatttctac ttctcactct
    12601 gactaacatc cgcgagcata tcgtcaacga tattcccgat tcactgaagc acggaaccgc
    12661 agccggcata ggctttttca tcgcgttcgt agggctgcgg aatgcaaaaa ttattgtagc
    12721 gagcgcggct acgttcgtta cccttggaaa atcttccgat cctcaggtgc gcctggctgc
    12781 gcttggacta atcattatcg ccatcctgat gtcgcggaaa gttggcggcg ctattttgat
    12841 tggcatcgtc gccattgcac tggccggaat cccgctgggt ctgacgacag taccttcaca
    12901 tattttttca tggccgcatc cgggcggaac attcctgaaa ctcgattttc gctcggcgtt
    12961 caaaatcggc cttggcgatc tggttttcgt ttttttcttt gtcgatttat ttgacaacgt
    13021 gggaacgctg gtcggagttt gcgagcaggg cggatttttg cgcgacggaa aattcccccg
    13081 cgcgagtcgc gcactactcg ccgacgcatt cggcacaatg gccggagcgc tgactggaac
    13141 gtccacagtc acaagttata ttgagagcgc tgctggagtc gcggccggtg cgcgaacggg
    13201 actaggcaac atgttgatcg cagcgctttt tgtggcggca attttctgcg cgccgctcgt
    13261 cgccgccgtg cctacgtatg ccactgcccc agctttaatt ttggtcggcg cgctcatgtg
    13321 cgcatctctg gcaaaagtag attgggacga tttcacggaa gcgttccccg cgtttatcac
    13381 caccgtagcc acgccgctga ccttcagcat cgccacaggc ctcagcctgg gcttgctttc
    13441 cttcactttc atcaaaattg gcacaggaaa aataaagcag atcagtccgc taatttgggt
    13501 gctatccgta ctattcttgc tacgtttcgc tttcctcggc gccgaataaa cgaatagcgt
    13561 ggtctcaagt ggcgtgggct tcagagtctg tgtgagagct gcagcaatgg aacctcagcg
    13621 gctattgatg actgcataac taatgtccgg gcactcctgc ccaaaaggcc cctaaaagag
    13681 ccaaaaactg acggtatgca taggacatta gaaatgcagc cctcaataaa accgaactga
    13741 ttttgcagga cttatgtcgt ggctaaagcc tcgacccaca acaattctgc agttttcaca
    13801 cagactcttc agcctgcgga gtttagtcct tgcaagaccc cgaggctgaa gcccgcgtca
    13861 ttagtttcga ccccacttcc ttagctcaat aacgcactgt caaaaaattc tcggtatcgc
    13921 tccccttgaa tagaaacaat caagaagaaa acacgccttc gttacatctc ggaatagtaa
    13981 aattaaaatt atcattgcgg agtacgcatt taaatttata cgcactgtcc tatgcctacc
    14041 caaatagtcg aggatgaggc tgtcccgcag tcgataacga ctgaaattcc tgcatttggc
    14101 cgcaaagcca ttcaatattt catcagtttt tttttgtgct tcattgtact ggctgtgttc
    14161 atccctctta gtccgttgat gcccgcgcag ggcctggata catcttggat gtttgccatg
    14221 aaccaggggg tcgcgcatgg atttgtgttt ggcaaagata ttgtattcac atttggccct
    14281 tattcttcca tctatacaga gctttacgat ccagcgaccg ataaactgat ggtgtttggc
    14341 agtttttttc tggggatatg ctactgtttt cagttgttgc ttttagcaaa acacaaaagc
    14401 aattacgggt tgttgtttta tggaatattt ctcgcatgcc ttatggattc gcgagacgcg
    14461 ctcttatttt cttatccatt aattttgtcg ctcgttgtct accgtgtgac gttgccgcac
    14521 gaacacgctt ggaaattgca tcttgaaaac tctctctctt atggcgttgc cttgctcttc
    14581 gcaccgcttg gattacttcc gcttatcaaa ggttcccttc tgccaatgtg cggaatgacc
    14641 gcgttccttt gctttgcgat tttttggtta aacgacaaga agattctggc ctgggctaca
    14701 gtgggcattc ccgcaatttc gtgtgcgttg ttctggcgca tatccgggca atcgctattc
    14761 gccttgcccg gttttttttg gaatatgcgg caaattattt ccggttataa cgaagcaatg
    14821 tcgttccccg gcaactcggg cgaatgtgta ctatatattc tggctgccgt tttaattatt
    14881 tttgcgatcg cctcgacaat tcgcgcgcca aggatttcca catcgtttct atccatttca
    14941 tatgcgtttt tcttgtttct cgcatttaag tccggattta tcaggcatga tccatggcat
    15001 aacatcatag ccgg
//
LOCUS       DASZMB010000167        13739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2376343, whole genome shotgun sequence.
ACCESSION   DASZMB010000167 DASZMB010000000
VERSION     DASZMB010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 13739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13739
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2376343"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..564)
                     /locus_tag="VG272_07590"
     CDS             complement(178..564)
                     /locus_tag="VG272_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13582.1"
                     /translation="MRSSCLLRRSSPFSEGAFSYCERLCASYYSSLAFKIVFGELRKQ
                     GAELLTYQATFMVLKAALKDSHPGFAPLADATLTHARVSPAVLENVRRLFDEPLEKFL
                     QQVAQAETEEEVEKLLLAMPTNNWVN"
     gene            563..958
                     /locus_tag="VG272_07595"
     CDS             563..958
                     /locus_tag="VG272_07595"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HWF13583.1"
                     /translation="MTHSLPDGLTSILSNHSVDQTLANLQNVLHAKNIAVFAIVDHSG
                     EAEKAGMAMPPTKLVLFGSPKAGTPLMLASPSVAIDLPLKILISEDHEKKIWVSYNSL
                     KFLQDRHSLPEQLMQNLAAVEAVAAAITQ"
     gene            complement(982..2784)
                     /locus_tag="VG272_07600"
     CDS             complement(982..2784)
                     /locus_tag="VG272_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13584.1"
                     /translation="MKLKVCGAPIRCQGKRLPVPGGVLVALLACVLAIFVPPVQAQLK
                     TKAEVGVNVGANCSDSGPCYTNAPVIGDLPPIGKPVVFNDEKCLPWNLSATQAGTSHV
                     TALKVPSGARGEFQKACAASQKKKLEESEQHLRRAIDKFKDYSVAWVMLGVVLDERHK
                     DQDARDACSHVTTADPKYFPAYLCLAEFSARNQEWDKLLDLANTALSISSENDGYAYY
                     YRAMANFYLNRVDDAKKDALKAAEVDLDHNYLPLYFLLAQIYAAQGDRAAAAAQLRVA
                     LKNHNDPAQEDVAKRYLAKLEAIDTTPIFQNAAASGGAARTISLTPDDSDIASMSDVR
                     TVDDNWIPEDIDHAVPPVDTASACSLPSVLDGAGKKIVELVHNVDRFTATEVLMHQPV
                     DHLGHLGPAVTTQFSYLVSYTEEAKGPLHVDEFRNGSLSIDPFPNHIATVGTPSLVLI
                     FHPRYAGNFKMECEGLGQWRGQPAWQIRFEQRTDRPNLTYSFSVDRATYAVNLRGRAW
                     ILANSYQVARLETDLIHPIPKIRLRLNHQSVEYRPVKSPSGKMQLWLPSSTELYMDFQ
                     GRRFYRKHSFTDFKIFSVDSQYRVAERKKIDPEE"
     gene            3253..4431
                     /locus_tag="VG272_07605"
     CDS             3253..4431
                     /locus_tag="VG272_07605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWF13585.1"
                     /translation="MQDIRAANVTMRILRSVAELETVREFWSSTPGTRDSDIDVFFPE
                     GQRGNSDALLPRVLVLYRDDKPVAMLIGKIVRRESVFRVGYFQLGKIVADVLTIPYGG
                     MRGEGSKENCEVFVRAIQACLKKGEADVALFQYVNANSAMFRCVKREPNFFSRDHFTP
                     LRPHRMRKLSPHVDKLYAGATQGGKQLRRVSNKLMAEFSGEIKVDRLEDLADMDRTLA
                     VIEDIAKKTWQRKISNIGFNLEDASLMHLLKVEARGGHLRVYTLYLRGTPCAFWIGAV
                     YRRTFISDFLGYDPDFANYSPGTYLLSQMMEDFCSQGVEEIDFGFSDEEYKRRFGNVV
                     WEDASVHVFAPNWRGLRLSFLRMITAVPHEAARAFLKRTDLYQKAKKLWRKIGRRKKD
                     "
     gene            4746..4819
                     /locus_tag="VG272_07610"
     tRNA            4746..4819
                     /locus_tag="VG272_07610"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4778..4780,aa:Cys,seq:gca)
     gene            complement(4887..5177)
                     /locus_tag="VG272_07615"
     CDS             complement(4887..5177)
                     /locus_tag="VG272_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13586.1"
                     /translation="MVGILVMGDNHLIVRGPFPDRETALALAQHWRLIQIGRTTPVHL
                     QQWSICTREHRENLEWAISVPGDGEISAAVRELLDELSARGISIFDSRSATW"
     gene            complement(5184..7073)
                     /locus_tag="VG272_07620"
     CDS             complement(5184..7073)
                     /locus_tag="VG272_07620"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HWF13587.1"
                     /translation="MTRFLSRALKDSQLAGLNHETQRLFAYHQATKYTYQSVRASAHF
                     LDWRNQPNPFRTYDGAQLVALPRDPDFPDMGTFVAMGALDEAATTAEKSDPAERPAVA
                     LDATCLSRLLWHSMAVSAWKKIPGSGSRYSLRVNPSSGNLHPTETYLVLRGMEDLRDG
                     LYHYRADMHALELRGEGARTQQFAQALEFPHAADASLIVGLTSIFWREAWKYRERAYR
                     YCCHDLGHAMMSILLSARTLGLSGGAIGHFSDSRMTNLLGLTGSDEAPMAFLVFPTGK
                     IPTGSAPSDSATAEKFVGEPNELSAEEAPYKLLLDMHASTVSPDPAGALPHACLSNAN
                     ATDRLPKEFENAREGTGDSSFGMIARRRRSALDFDPRTSPMPRADLQQLLEFATRDWR
                     ADWRGNFEGNTFSLPATGADFVTLYLFVHRVHDCESGVYIWDGRRRALQQLHQGNVQR
                     VAAFLSLEQTLAGNSCFTVSMVADLDKAAITFGNRGYRYVYFEAGAIGQRIYVGAEAL
                     GWNATGIGAFYDDDVHRYLGFLAEIDGPVSNVDQAQGSTLVMLDSPASRHAMEQKHEA
                     KEVASAIAAANVQALPTGRRSPKDYLMTAQMGAALPTDSSKLPRQVIYHFAVGRAIPD
                     PRIED"
     gene            7575..7991
                     /locus_tag="VG272_07625"
     CDS             7575..7991
                     /locus_tag="VG272_07625"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWF13588.1"
                     /translation="MIKNSIKFPILILALATVASFALIQAAPAPQQPTVSRKILLKED
                     AAVPGYEQAMVAVEIPVGGREGRHTHPGSAMIYVLEGTLTLDYEGKATGTYNPGDSIY
                     VEPGKIHEGINNGKAPIKVVATFLLKKGEPMTSPAK"
     gene            complement(8215..9108)
                     /locus_tag="VG272_07630"
     CDS             complement(8215..9108)
                     /locus_tag="VG272_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWF13589.1"
                     /translation="MNSLSKPTRRGLVKWGLGAAGLPLISSVLAATAQGSAKPEHANR
                     RVGQTLALASGVSVYYKEEWLGAPWLDSDPVVFLHGNLETNEIWYGWVPQMAQHYRLF
                     RPDLPGFGGSTAPANFEFTLSGFAKFVADFLDALGIQSAHIVGAKTGGGIAMQFAATY
                     PQRTRSVVVASGPFVALGPKVEQNSQQMRLGTAATAEEMAYFDKMRDDMRPETKKQME
                     ILLGMLNVDDTLGRITSPTLIITSDQSALQTVDTVLRYKSKIANSRLLVLSSDAYHVA
                     VANADECVANVLTFYRQSVKR"
     gene            complement(9278..11746)
                     /locus_tag="VG272_07635"
     CDS             complement(9278..11746)
                     /locus_tag="VG272_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13590.1"
                     /translation="MLVGTSIYRLNFKLLSYCLALLIVIAGISGTSFARTDNVFDLTG
                     PQIEVNVTHAGKRLGIADVPNLMPGDRLWIHVNFPDNQAVHYLLVVAFLRGSTNPPPE
                     DWFTRAETWNKQMIAEGIVVTVPKDAQQALMFLAPETGGDFSSLRSAVRSKPGVFVRA
                     TQDLNQASLDRTRSNRYLAEIKNAAELDPTALHDRSVLLSRTLGMKLDEKCFDRPAEE
                     QSTCLTQNTDQLVLDDGHTESMVAALTSGPATDLMGALSATSMAGGGFYSAYVGAVVD
                     LARILSNLHTAAYQYIPALAVPKGGELNLRLNNPPSFRTPKSVLVIGLPAVEAAQLPP
                     LRAVNAEQILCLEKLPLVLPVDGAPLVFSTDIAHDFALRIKKKDGAVIELPAAQDPTR
                     GGFVVDARALSGAEFNTDVSATLHGNWGFDPFEGPAFHLRNAHQTKWTVMPADQGALV
                     VGRVDTVHLQSDCAVCVEKISVQDDQHRDVETSWKTVKSDQLEIQVTLKDQRVGPLKM
                     AVKQFGANASDELVLNSYAEAAELDHFKINAGDKQGVLTGTRLDEVNSFELKGVRFVP
                     TKLSRADKKDELQLAVATPAPHATPFQPDEKLVAHIDLKDGRILDLQTTVDKPRPAVT
                     LISKNVQPATRSSVHLKNQDELPQDARLSFFLKTEVPESFPRTEKIEVSSADDSFHVV
                     LSLGDGNLILQNSETVLAILDPLKSFGPSAFGPLRFRPIDNDRENGDWQPLVTLVRIP
                     TLKELRCPESPDKQCKLIGSSLFLIDSVASDPQFNHAISVPVGFPDAPLSVPRPNGTL
                     LYIKLRDAAGTVDSLVLPVLPDDE"
     gene            complement(12111..12311)
                     /locus_tag="VG272_07640"
     CDS             complement(12111..12311)
                     /locus_tag="VG272_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13591.1"
                     /translation="MRCRDTAAVLPLLATERVRRAIQRCAAVTRDLQDGKLTAEVTGI
                     ESLRRATAQLSKDLAELFVADR"
     gene            12478..>13739
                     /locus_tag="VG272_07645"
     CDS             12478..>13739
                     /locus_tag="VG272_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HWF13592.1"
                     /translation="MKPLNLALSLVFSLAFSISGNAQQTEPLRTVRTIELPAAVKGSF
                     DHLAADVAHNRLFVAAEDYQAVLVVDLANGTVAHEIKGIARPHAILYREDVNRIYVTD
                     GADGSVKVFDGATYQALSTVSLAKDADSIGYDVSRQWLYVDNGGKSEGKKFSMLSVID
                     TASANKLADIKIEGDTLEAMAVDVFRLRMYINNKAKNEIEVVDRWKGAVIASWPVTMC
                     KENVAMGLDEQRQRLFVGCRDGKISVFDTNTGKELQALDASKDMDDVVYNADTKRIYS
                     VGGGAVSVLEQTDADHYHAVGSVPTARGAKTAILVPALSRYFVAEPQGNSKNAAILEM
                     EPVGVPAARAAEPEVSLSVEAPAAEQLILKTMSAHPYLRKMGIHAIPPGATASVIIAN
                     AYASRIGVKSSDGDLEAVKGDNIYCVKR"
BASE COUNT         3175 a         3804 c         3699 g         3061 t
ORIGIN      
        1 agacggcgca tttgggcatt tgggacaagt tttttgcggc atccgttccc ccattgaagt
       61 cgaagtctag taaatgtgtc tgtttgtaac gagtaccgca taagctacta cgcggactgc
      121 tataaatgcg agggtaaaat catatgactg atacaaaagc gacaggcgca atggacgcta
      181 gttcacccag ttgtttgttg gcattgctaa gagaagtttt tcaacctctt cctcagtctc
      241 agcttgagcg acttgttgaa gaaatttttc cagaggttcg tcaaataagc gccgcacgtt
      301 ctccagaaca gcgggtgata ctctggcgtg agttagcgtt gcgtctgcaa gcggagcaaa
      361 gccgggatga gaatctttca gcgcagcttt aagaaccatg aacgttgcct gataggtaag
      421 taattcagcc ccttgctttc tgagttctcc gaatactatt ttgaacgcaa gcgaggaata
      481 gtaggatgcg cagagacgtt cgcaatagga aaacgcgcct tccgagaagg gtgatgagcg
      541 acgcagcaag caggaggacc gcatgacaca ctctctcccc gacggactca catcgattct
      601 cagcaatcat tccgtcgacc agacgctggc caatcttcaa aacgttttgc acgcaaagaa
      661 catcgctgtg ttcgccattg tcgaccacag tggcgaagcg gaaaaggcgg gcatggcgat
      721 gccccccacg aaactcgtcc tcttcggcag cccaaaagca ggaacgcccc tcatgctggc
      781 ttcgcccagc gtggcgattg acctgccact caagattctg atttccgagg atcacgagaa
      841 aaaaatatgg gtgtcgtata acagcctgaa gtttctgcag gaccggcatt cgctgccgga
      901 acaactgatg caaaacctgg ccgcggttga ggctgtcgcc gcggccatca ctcagtagag
      961 cgaaagtcgc tgattcacac tctattcttc cggatcgatt tttttgcgct cggccacgcg
     1021 atattgcgaa tccacggaaa atattttgaa gtccgtgaag gaatgttttc tgtagaaacg
     1081 ccgcccttga aaatccatat agagttccgt gcttgaggga agccacaatt gcatttttcc
     1141 cgaaggcgat ttcacggggc gatactccac gctctgatgg ttcaggcgaa gccgaatctt
     1201 ggggattgga tggataagat ccgtttccag ccgcgccacc tggtaggagt tcgcaaggat
     1261 ccaggcgcgt ccgcgcaagt tcacggcata cgtagcgcga tcgacagaaa acgaataggt
     1321 aaggttcggg cgatccgtgc gctgctcaaa gcgaatttgc cacgcgggct gcccacgcca
     1381 ctgtcccagt ccctcgcact ccattttgaa atttccggcg taacgcgggt gaaaaattag
     1441 aaccaggctc ggcgtaccga ccgtggcgat gtggttcgga aacggatcga tcgacaggct
     1501 cccattacga aactcgtcga catggagcgg ccccttggcc tcttccgtgt acgaaaccag
     1561 gtaactgaat tgagtggtga cggcgggacc caaatgcccg aggtgatcga cgggttgatg
     1621 catcaaaact tcagtcgctg tgaaccgatc aacgttgtga acaagttcta cgattttttt
     1681 tcccgctccg tcgagcacag agggcagcga gcatgctgat gccgtgtcga caggcggcac
     1741 cgcgtggtca atgtcctcag gaatccaatt gtcatccacc gtccggacgt cagacatgga
     1801 ggcaatatcc gagtcatcgg gagtgagcga aatggtccgc gcagccccac cggaggctgc
     1861 cgcattctgg aaaatggggg tcgtatcgat cgcctcaagt ttggccaggt aacgtttcgc
     1921 cacatcttcc tgtgcaggat cgttgtgatt tttcagagcg acacgcaatt gcgccgccgc
     1981 agcggccctg tcaccttgcg cggcataaat ttgcgctagc aggaagtaaa gcggcaaata
     2041 gttgtggtcc agatctactt ctgccgcttt taaggcatcc ttcttagcgt cgtcaacacg
     2101 gttcagataa aagttggcca tcgccctgta gtaatacgcg tacccatcat tctcggaact
     2161 gatgctcaga gccgtattcg ccaaatcgag tagcttgtcc cactcctggt tccgcgctga
     2221 aaactcagcc aggcagagat aggcaggaaa atatttcgga tcagccgtcg ttacatgcga
     2281 acaggcatcg cgtgcatcct ggtctttgtg gcgctcatcg agaaccacgc ctaacatgac
     2341 ccatgcgacg gagtaatcct taaacttatc aatggcgcga cgcaggtgct gctcggattc
     2401 ctcaagcttt ttcttttgag aagcagcgca tgccttttga aactcgcccc gagccccgga
     2461 tgggactttc agcgccgtaa catgcgacgt tcccgcttgc gttgccgaaa ggttccaagg
     2521 caggcatttc tcgtcattga agacaacggg cttgccaatc gggggtaaat cgccgatcac
     2581 cggagcattc gtatagcaag gtcccgagtc gctgcagttt gcgccaacat ttactccgac
     2641 ttctgccttg gtctttaatt gcgcctgaac agggggaaca aatatggcca gcacacacgc
     2701 gagcaacgcc acgagtacgc caccgggaac tggcaaacgt ttcccctggc aacgtatcgg
     2761 cgcaccacat acttttagct tcatgactcc gccttaagac ttaactcgag atcgccgccg
     2821 gcgatcagtt tccctattga tagggtaatg ctctgatgcg agttagtgga aatactgcga
     2881 acagtttatt tacagctttt cgagcccctc gaatttcggt taccggtccc gagatcgaag
     2941 tgtgtgtggc gattttcgcg cggaagggcg cacgtgtcaa tcgcggtgcg tggctctatg
     3001 ctaagccgtt aaaagtgtac gggggtgact cgtccgcgat gttttgatgg cttggctaag
     3061 cgcctgaccg gcagacgccg cgctttttgg agccctgagc ccaaattaat ttccgtttgg
     3121 gacaataccg ggcgatcaaa ttttgcatcg cgttacccat agaagccaca aatcgcgccc
     3181 aatattgcgc gcggtatgtg atatgaattc agcggcggcg atccgaccga cacgatttga
     3241 gcgagcgtca ctatgcagga cattcgtgca gcaaacgtga cgatgagaat tctgcgcagt
     3301 gtcgcggaac tcgaaaccgt gcgtgagttc tggtcttcta ctcctggtac tcgcgattcg
     3361 gacatcgacg tcttttttcc ggaaggtcaa cggggaaact ctgacgcgct cctcccgcgt
     3421 gttctggttc tctaccggga cgacaaacct gtagctatgc tgattggaaa aattgtccga
     3481 cgcgagtccg tattccgtgt cggatatttt caacttggta aaattgtggc cgacgttctg
     3541 accatacctt acggcggcat gcggggagaa ggatcgaagg aaaattgcga agtatttgtg
     3601 cgcgcgattc aggcttgtct gaagaaggga gaggctgacg tcgcgctatt tcagtatgta
     3661 aacgcaaact cagccatgtt ccgctgtgtt aagcgcgaac ccaacttttt ttctcgcgat
     3721 cattttacgc cgctcaggcc acaccgaatg cgaaagcttt cgccgcacgt cgataagctt
     3781 tacgccgggg cgactcaggg aggcaagcag cttcgccggg tttcaaataa gttgatggcc
     3841 gaattttccg gagagatcaa ggtggatcgg ctcgaggatt tggcagatat ggatcgcacg
     3901 ttggccgtga ttgaagacat agcgaagaag acttggcagc gaaaaatcag caacatcgga
     3961 ttcaacctcg aggatgcttc cctgatgcat ctactaaaag tagaggcacg agggggccat
     4021 cttcgtgtgt acacgctcta tctgcgggga acgccatgcg cgttttggat cggcgcggtc
     4081 taccggcgca cctttatcag cgatttcctg gggtacgatc ccgatttcgc caactattcg
     4141 ccgggcacgt acctgctttc acagatgatg gaggattttt gctcccaagg cgtcgaggaa
     4201 atcgactttg gtttcagtga tgaggagtat aaaagacgtt ttggcaatgt cgtgtgggag
     4261 gacgccagcg tacacgtgtt tgctcccaat tggagggggc tcaggttaag ctttttgaga
     4321 atgattacgg ctgttcccca tgaggctgcg cgggctttcc tgaaaagaac cgatctttat
     4381 cagaaagcga aaaaactatg gcgaaaaata gggaggagaa aaaaagacta gacacccgcc
     4441 acgtctcgca ggattccctc agccttgagt gaaccgccca tcgtgcgaag cggcctggga
     4501 aggaaaatga tcgcctgacc ccgagaagcg tctccgaagt tgaagaactc cgcgacacat
     4561 gaaccaattc acgttcacaa cttttctaaa gattccaaga cctctccctt aaccgcgaaa
     4621 tgctttttta gggctactat aaaacgcgct tcaatggcgt atcaaaggca cagaatcatc
     4681 gacacccgac ggaatgagag aatatgctta gagaggcggc aagagtgaac tccatcgggc
     4741 ctcggggcgc ggtagccaag tggtaaggta aaggtctgca aaaccttaat cggtggttcg
     4801 attccacctc gcgcctccat tttttacacc tggttaatct atgaaggccc tttgcacggc
     4861 gcgcagctcg tattttacgg acaggctcac caggttgcac ttcgcgagtc gaaaatcgaa
     4921 atccctcgcg ccgaaagttc atccagcaat tcccttacag ctgccgaaat ttcgccatcg
     4981 cccggcacgc tgattgccca ttcgagattc tcccgatgct cccgcgtgca gatggaccat
     5041 tgttgcaggt gcaccggcgt tgtccggccg atttggatca ggcgccagtg ttgcgccaac
     5101 gccaaggccg tttcacgatc cgggaacggg ccgcgcacaa tcaaatggtt atcgcccatc
     5161 accagaatgc cgaccatgta acttcaatcc tcaattcttg gatcgggaat cgcgcggccc
     5221 accgcgaagt gatagatcac ctgccgcggc aatttgctgg aatctgtggg cagcgctgcc
     5281 cccatctgag cggtcattaa ataatctttt ggtgaacgtc gcccggtggg aagtgcctgg
     5341 acatttgcag cagcgatcgc cgacgcaact tctttggcct cgtgtttttg ttccatcgcg
     5401 tggcggctcg ccggagaatc cagcataacc agcgtcgatc cttgcgcttg atcgacatta
     5461 ctaaccgggc catcaatttc cgccaaaaat cccagatagc ggtggacatc atcgtcataa
     5521 aaagcgccga ttcccgtggc attccaaccc agcgcttctg ctccgacata aattctctgt
     5581 ccaatagccc cggcctcaaa atatacgtac cggtaaccac gattgccaaa tgttatggcc
     5641 gccttatcca agtcagccac catagaaact gtgaaacacg aattgcccgc gagcgtctgc
     5701 tccaaactca gaaaggccgc cacgcgctga acatttccct gatggagttg ctgcaaggcg
     5761 cgacgccgtc cgtcccaaat atagacgcct gattcgcaat cgtggacgcg atgaacgaaa
     5821 agatataacg tgacgaaatc cgcgcccgtc gcgggtaacg aaaacgtgtt tccttcaaaa
     5881 tttccgcgcc agtccgcgcg ccaatcccgg gtcgcgaatt ccagaagctg ctgcaggtcg
     5941 gcccgcggca tcggagaagt tctaggatcg aagtcgagcg cagaacgtcg cctccgcgcg
     6001 atcattccaa aactgctgtc acccgttcct tcccgcgcat tttcgaattc tttgggaagg
     6061 cggtccgtcg cattcgcatt cgataagcac gcgtgcggca gcgcccccgc cgggtctggc
     6121 gaaacagtcg aagcgtgcat gtccagcaag agtttgtatg gcgcttcttc cgcagacagc
     6181 tcgttcggtt ctcctacaaa cttctccgcg gttgcggagt cgctcggcgc ggagcccgtg
     6241 ggaattttcc ccgttggaaa cacgagaaaa gccatcggcg cctcgtcgct cccggtgagg
     6301 ccaagcaggt tcgtcattcg cgaatcgctg aaatggccga tcgcgccgcc ggacaaaccc
     6361 agcgtgcgag cagaaagtag aatgctcatc atcgcgtgtc ctaaatcgtg gcagcaatag
     6421 cgatacgcgc gctcgcgata cttccacgcc tcgcgccaaa aaatcgaagt tagtcccacg
     6481 ataagcgatg cgtcggccgc atgcggaaat tcaagcgctt gggcaaattg ctgggtccgc
     6541 gcaccctccc cgcgcaactc cagtgcgtgc atatcggcgc ggtaatgata cagtccgtcg
     6601 cgaaggtcct ccattccacg cagcaccaaa taagtttccg ttgggtgcaa atttcccgag
     6661 gatggattga cgcgcaaact ataacggctg cccgagcctg gtattttttt ccacgcgctc
     6721 acggccatgg aatgccacag caatcggctg agacatgtcg cgtcaagcgc gacagcgggg
     6781 cgctcagccg gatcactttt ctcggccgtc gtggctgcct cgtcgagcgc ccccatcgcc
     6841 acaaacgttc ccatgtccgg aaagtcagga tcacgcggca gcgcgactag ttgtgcgccg
     6901 tcataggtgc gaaatgggtt gggttggttg cgccagtcaa ggaaatgggc gctggcgcga
     6961 acggactgat aggtatattt agtcgcttgg tggtaggcaa acaagcgctg agtttcatgg
     7021 ttgagccctg caagttggga atctttcagc gcacgagaaa gaaagcgcgt cattttgcga
     7081 tccgcgagaa acctgtatag ctcgtcactt tatgtctcgt ttcggctcgg tgggcgaaag
     7141 taatactggt agacatcacg tctgccccga tctccgatga tagcattgcg cttgccccgc
     7201 aaatagagag tcgccaggac atccacgccg tacggcacaa tgctctctcc gtcccgaaaa
     7261 acacgggacg ggagagggtg cccatggcca ccaccggagt tacaaacaac tcaaacgcgc
     7321 agtcctgtag cccgccgctt cacaggcgcg ctacaacaac tgtctttgcg ggtcgcaatt
     7381 tcactcgcga cgttcaagcg ctacaagttg agggcttcta gttcctgaag tccaaccgct
     7441 cgacccagcc aacttcgatc aaagattgag ttgtgtgact ccgcctgctc gaagtagtct
     7501 tagggccgcg gacaaggcta aaacggcgca atacgttttg tgagcctgcc gactctctgg
     7561 gtgagaaaac atcgatgatt aaaaattcaa taaagtttcc aatattgatt ctggctctgg
     7621 caacggttgc gagcttcgcg ctgatccaag ccgctccagc accgcagcaa ccaactgtct
     7681 cccggaaaat tctactcaaa gaagatgcag cggtgcctgg ctacgagcaa gccatggtcg
     7741 cggtggaaat tccggttggc ggacgcgaag gccggcatac tcatcctggg tcggccatga
     7801 tctatgtgtt ggaaggcacc ctcactcttg actacgaagg aaaggcaacc ggcacttaca
     7861 atcccggaga ctccatctac gttgagcccg gcaaaattca cgaaggcatc aacaatggga
     7921 aggcgcccat aaaagtcgtc gccacattcc ttctgaaaaa gggcgaaccc atgacgagcc
     7981 cggcgaaata aattcgcgca gctgaaagca ggcgcagcga gggtccgtga ccgaatcctc
     8041 acgcagccgc gaccgcgtag acttttaata agcacatttg gcacgaagaa tgatcggaaa
     8101 ttcctgggaa cgccaatctc ctgattggcg cattaccccg gcttccgtcg caaaaacgcc
     8161 aatcgggaga ttggcgttcc caggaatttt gagaacgcga gccagcagcg attgctagcg
     8221 ctttactgac tgtctgtaaa atgtgaggac gttcgcgacg cattcgtcgg cgttcgcgac
     8281 ggccacatga tacgcatcgc tgctcaaaac cagcaatctt gagtttgcaa ttttcgattt
     8341 gtaacgcagc actgtgtcca cagtttgcaa tgcgctttga tctgaggtaa tgatcagcgt
     8401 gggcgaggtg atgcggccca aagtgtcatc cacgttgagc atgccaagca aaatttccat
     8461 ttgctttttt gtttccgggc gcatgtcgtc gcgcatttta tcgaagtagg ccatttcttc
     8521 agccgtggca gcggtgccga gtctcatctg ttgggagttc tgttccacct tgggccccag
     8581 cgcgacaaat gggccgctcg ccacaaccac cgaccgcgtg cgctgcgggt acgtcgccgc
     8641 aaattgcata gcgattccgc cgcctgtctt cgcgcccacg atgtgtgcgg actggattcc
     8701 cagcgcatcg agaaaatccg ccacaaattt cgcaaatccc gatagcgtaa attcaaagtt
     8761 ggcaggcgcc gtcgaaccgc cgaatccggg aaggtccggc cggaacaacc ggtaatgctg
     8821 agccatttgc ggcacccaac cgtaccagat ttcgttggtc tctaaattac cgtgcagaaa
     8881 cacgacagga tcggaatcca gccacggcgc acccagccac tcttccttgt aatagaccga
     8941 aactccggaa gctaatgcca gggtttgccc gacgcggcga ttcgcgtgct ccggtttcgc
     9001 gcttccctgc gctgtcgcgg ccagtacgct cgaaatgagc ggcaatcctg ccgcaccgag
     9061 tccccacttg accagtcctc ttctcgtcgg cttcgacaat gagttcatac tcttactccc
     9121 tccgctttga cgacgacgac agccccgtgt ttcaatccgg attttcgact ttgcagagca
     9181 atgcttaagc ggccccatct tagcaaggtc ttccctgctc ggccaccatc ggataccgat
     9241 ttttaaattg aacgagtgaa cgacgctctg tgaaagatta ttcgtcatcc ggcaacactg
     9301 gcagaaccaa agagtccacc gtgcccgcgg cgtcgcggag tttgatgtag agaagcgtgc
     9361 catttgggcg cggcacagac agcggcgcat ccggaaaacc caccggcacg gagatcgcgt
     9421 gattgaattg cggatcggaa gcgacagaat cgatcaggaa taggctcgat ccgatcagct
     9481 tgcactgttt gtcgggactt tccgggcagc gtagttcctt caacgttgga atgcgcacca
     9541 aggttaccag tggctgccag tctccgttct cgcgatcatt atcgatcggc cgaaaacgca
     9601 gcgggccgaa cgcggatggc ccaaaacttt ttagcggatc gaggatcgcc agcacggttt
     9661 ctgaattttg caggatcagg ttgccatcgc caaggctcag caccacgtgg aaggaatcat
     9721 cagcgctgga cacttcgatc ttttccgtac gcggaaaact ttccggcacc tccgtcttta
     9781 aaaagaacga aagcctggcg tcctgcggca gttcatcctg atttttcagg tgcacggagg
     9841 atcgagtcgc cggctgcacg ttcttgctga ttaatgtcac ggccgggcgg ggcttgtcga
     9901 ctgttgtctg caagtccaga atgcggccat ccttcagatc aatgtgcgcc accagctttt
     9961 cgtcaggctg aaacggcgtg gcgtgcggag cgggagttgc caccgccagt tgcagctcgt
    10021 cttttttgtc ggcgcgcgat aatttcgtgg gcacgaagcg gacgcccttc aactcgaagc
    10081 tattcacttc gtctaaccgc gttcctgtca ggaccccctg cttatcaccc gcattgatct
    10141 tgaaatggtc cagctcggcc gcttccgcgt aggaattcaa aacaagttcg tcggaagcgt
    10201 ttgctccgaa ttgttttacc gccatcttca gcggcccgac tctttgatct ttcaacgtca
    10261 cctgaatttc cagctgatcc gatttcacgg ttttccagct tgtttcaaca tccctgtgct
    10321 gatcgtcctg cacgctgatt ttttccacgc atacggcaca gtccgattgc aaatggacgg
    10381 tgtcgacgcg acccactacg agcgcgccct gatcggcggg catcacagtc cacttcgtct
    10441 ggtgggcatt tcgcaaatgg aatgcgggac cttcgaaggg atcaaatccc caattcccgt
    10501 gaagcgttgc gctcacgtcc gtattgaatt cggcacccga cagcgcgcgg gcgtcgacaa
    10561 caaatccgcc gcgtgtcggg tcctgcgccg cgggcaactc aatcacggcg ccgtcttttt
    10621 tcttaattcg cagagcaaaa tcatgcgcga tgtccgtgga aaacaccaag ggcgcgccat
    10681 cgacgggaag aacgagcggt aatttttcca gacacaatat ttgttccgca ttcactgcac
    10741 gaagtggggg aagttgggcc gcctccactg ccggcaggcc tattacaagc accgattttg
    10801 gcgtgcggaa ggatggcgga ttattcagcc ggaggtttag ctcgccgcct ttcggcaccg
    10861 caagggcggg aatgtactgg taagccgccg tatgcagatt gctcaagatt cgcgccaaat
    10921 ctactaccgc accaacgtaa gcgctgtaaa atccgccgcc ggccatggat gtggcgctga
    10981 gcgcccccat caaatctgtc gccgggccgg aagtgagcgc ggctaccatg gactccgtgt
    11041 gtccgtcatc caagacgagt tggtcggtat tctgcgtgag gcaagtcgat tgctcttccg
    11101 cgggccggtc gaaacatttt tcgtccagct tcatgcccag agtacggcta agcagcaccg
    11161 agcgatcgtg caacgctgtc ggatcgagct ccgcagcgtt ttttatttcg gcgagataac
    11221 gattcgaccg cgttcgatcc aggctagcct gattcaaatc ctgcgtggcc ctgacaaaca
    11281 ctccgggctt gctgcgcacg gcggaacgca agctgctgaa atctccgcct gtctccgggg
    11341 ccaaaaacat cagcgcttgc tgggcatctt tcggaaccgt gaccacaatt ccttcggcga
    11401 tcatctgctt gttccacgtc tcagctctcg taaaccaatc ttcgggcggc ggattcgtag
    11461 acccccgcag gaaagccaca accagcaggt aatgcaccgc ctgattatca ggaaaattga
    11521 cgtgaatcca cagccggtct cccggcatca ggttgggcac gtccgcgatg cccagtcttt
    11581 tccctgcgtg agtcacattc acttcaatct gcggccccgt caaatcaaaa acattgtccg
    11641 tcctcgcaaa acttgtccca ctaatccctg ctatgacaat gagcagcgcc aaacaatatg
    11701 aaagcaattt aaagttaagc cgatagatcg aggttccgac cagcatatgc tctccctttt
    11761 tcaacaaaac aaatcagtac caccaaagcc gccaagagca ggattcggag aaaagataaa
    11821 agttcgtttt gattgttaca gccgggtggg caactgcgat tctacccaga aacaattgcc
    11881 gattcacatt cattttattt tgcgtgtttc gtgaagctaa ctcagggaag tccgccggca
    11941 attatcctgc acggagccga ttgagtgttg ttaacgatcg tcgatacaac gccatcgagt
    12001 atggaacatt agtctatgat ccgcagttat gacgcgggcg aaacggaagc attgaagctt
    12061 caatggattc cgaaaatcag tcgagtgcag ttcattgaca ccagttcatt ttatcgatcc
    12121 gcaacaaaca gttcagccaa gtccttggaa agttgcgctg tggcgcggcg caaactctcg
    12181 atccccgtta cttcggcagt gagtttgccg tcctgcagat cacgggtaac ggctgcgcag
    12241 cgttgaatgg cgcgacgaac gcgttccgtg gcaagtaacg gaaggactgc ggcagtgtcg
    12301 cggcaccgca ctcgacgctt cttgtgcgaa cacggtccgc ggaagcgcgg aactcctgta
    12361 aaatacgaga aataattttg tctgacttaa tatcttgttc aactttctgt aattctgcgg
    12421 acgccgcagt ggttttgttt aaattctatt cacatttctg tgctctaatc tgtcctcatg
    12481 aaaccactta atttggcgtt atctctcgtt ttctcgcttg cgttctcaat ttcgggaaac
    12541 gcgcagcaaa cggaaccttt gcgcacggtt cgcaccatcg aattgcctgc ggcggtaaag
    12601 ggctccttcg atcatttggc cgcggatgtc gcgcacaatc ggcttttcgt cgctgccgaa
    12661 gactaccagg ccgttctagt tgtggacctc gcaaacggca cggtggctca cgagatcaag
    12721 ggcatcgcaa ggcctcacgc aattctctac cgcgaagacg tgaaccggat ttacgtcacg
    12781 gacggggcgg acggtagcgt gaaagttttc gacggggcga cgtaccaggc gctcagcact
    12841 gtatctctgg caaaagacgc ggattcgatc gggtacgatg tgtcgcgcca gtggctctat
    12901 gtggataatg gcggcaagag cgagggaaag aaattttcaa tgctcagcgt cattgacacc
    12961 gcttcggcca acaagcttgc ggatatcaag atcgagggcg atacgctgga agcgatggcc
    13021 gtggacgttt tccggttacg aatgtacata aacaacaaag cgaaaaacga aatcgaagtg
    13081 gtggaccgct ggaaaggcgc ggtgatcgcc tcgtggccgg tgacgatgtg caaagaaaat
    13141 gtcgcgatgg ggctcgacga gcaacgccag cggttatttg tgggatgccg cgacgggaaa
    13201 atttcggtct tcgataccaa cacgggcaag gaacttcaag cgctcgacgc gagcaaggat
    13261 atggacgacg ttgtctataa cgcggatacg aagcgaatct attccgtcgg cggcggagca
    13321 gtgagcgtgc tcgagcaaac cgatgcggat cattaccacg ccgtgggaag cgtgccgacc
    13381 gcacgcggcg cgaagaccgc gattctggtt cccgcgctga gccgttattt tgttgcggag
    13441 ccgcagggca actctaaaaa tgcggcgatt ctggaaatgg agccagtggg cgtgccagca
    13501 gcgcgcgcgg ccgagccgga ggtttctctg agcgtcgagg ctcccgccgc ggagcaacta
    13561 attctgaaaa ccatgtccgc gcatccatac ctgcgcaaga tgggaatcca cgcaatcccg
    13621 ccgggcgcca cggctagcgt cattatcgcc aacgcgtatg ccagccgcat cggcgtgaag
    13681 tcgtcggacg gcgatctgga agccgtgaaa ggcgacaaca tttattgcgt aaagcgcga
//
LOCUS       DASZMB010000168         6768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3722277, whole genome shotgun sequence.
ACCESSION   DASZMB010000168 DASZMB010000000
VERSION     DASZMB010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3722277"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            321..623
                     /locus_tag="VG272_07650"
     CDS             321..623
                     /locus_tag="VG272_07650"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HWF13593.1"
                     /translation="MHLTPREIEKLMIATAAELARKRRARGLRLNYPEAVALITADLL
                     EGIRDGRSVSDLMEFGATILKREEVMEGVPEMVDEVQVEGTFPDGTKLVTVHHPIR"
     gene            730..1086
                     /gene="ureB"
                     /locus_tag="VG272_07655"
     CDS             730..1086
                     /gene="ureB"
                     /locus_tag="VG272_07655"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HWF13594.1"
                     /translation="MVPGEYLLAEGDIIANAGRREIELEIANTGDRPIQVGSHFHFFE
                     VNRALRFDRAAAFGMRLNIPAGTAVRFEPGDSKKITLVEIGGTKHVYGLNGLTNGPAD
                     EANKPNALKRASDHEF"
     gene            1147..2865
                     /gene="ureC"
                     /locus_tag="VG272_07660"
     CDS             1147..2865
                     /gene="ureC"
                     /locus_tag="VG272_07660"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF009686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HWF13595.1"
                     /translation="MSLKIPRRQYADLYGPTTGDRVRLADTELFIEIEKDLTTPGEEA
                     KFGGGKVIRDGMGQSSTATSADGVLDLVITNAVIVDHGGIIKADIGIRNGRIVGIGKA
                     GNPDIMNGVTSGMIIGAGTEVIAGEGKIVTAGGIDAHVHFICPQLVETALSGGLTTLI
                     GGGTGPATGTDATTCTPGIWNMHRMLESADGFPMNLGFLGKGNSSSPEPLREQIEAGA
                     IGLKLHEDWGTTPAAIDRALSVAEEFDVQVAIHTDTINEAGYVQDSIRAFKGRTIHTY
                     HTEGAGGGHAPDIIKVCGELNCLPSSTNPTMPFTVNTLNEHLDMLMVCHHLSPSIPED
                     VAFAESRIRAETIAAEDVLHDIGAISMMSSDSQAMGRIGEVITRTWQTADKMKKQRGA
                     LPGEKSGSDNIRVRRYVAKFTINPAIAHGISQEVGSVETGKLADLVLWKPAFFGVKPE
                     MIVKGGMIAWAAMGDANASIPTPQPVIYRPMYGSYGGAPHSTSLTFLSRAAVHSDLRD
                     KLGLKKKTVAVNHCRGLTKKNMINNDALPRIEVDADTYEVRADGVLLTCKAAQVLPMA
                     QRYFLF"
     gene            2814..3326
                     /locus_tag="VG272_07665"
     CDS             2814..3326
                     /locus_tag="VG272_07665"
                     /inference="COORDINATES: protein motif:HMM:NF017045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13596.1"
                     /translation="MQSRAGIAHGAALLPFLMWGNTLIIDHIAAAANPENLKGKQEDK
                     LVFTWEQRHWMRGRFTTAQGRKIGIALPTGTIPAPGTILYVGEDWYLKIEAAPEAVLE
                     ISFKDSAEAVKIAFEVGNLHFPLALVEGKILVPDDKAMVRLMERLGVCYERRQTIFHP
                     IGNSQLHQHG"
     gene            3319..4002
                     /locus_tag="VG272_07670"
     CDS             3319..4002
                     /locus_tag="VG272_07670"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HWF13597.1"
                     /translation="MDKFLSLLQFSDGLFPAGAYAHSFGLEACVQAGEVSDANGVESF
                     LRGYLEGCAAPTDVVAVLCAWRAAKTGSLNSCLSLDERLDAMKTPSELRDASRQMGRQ
                     TLRVATHLPCHSLLEEFAKSVAEENTPGHHAVIFGIIGGVLDWDAMDMTGAYLYSTSA
                     VLVGAALRLLPLGQLAGQRILWNVRPLIATLAAEAQSKTETDMWSFAPALEVASMRHA
                     LLDARLFRS"
     gene            3999..4610
                     /gene="ureG"
                     /locus_tag="VG272_07675"
     CDS             3999..4610
                     /gene="ureG"
                     /locus_tag="VG272_07675"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HWF13598.1"
                     /translation="MITKPLKIGVGGPVGSGKTALVEVLCSRLKSSYQLAVITNDIYT
                     REDAEFLTRRGVLPPERIVGVETGGCPHTAIREDASVNLEASSALMRRFPDLDILFIE
                     SGGDNLAATFSPELVDATFYVIDVAEGEKIPRKGGPGIMTSGMLVINKIDLAPYVGAD
                     LAVMDRDARRMRGERPYVFTNLKNGTGVDTLIDWIRHSLLLEQ"
     gene            4600..5460
                     /locus_tag="VG272_07680"
     CDS             4600..5460
                     /locus_tag="VG272_07680"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HWF13599.1"
                     /translation="MNSDFQGERIPNRVGRDGFLRLRFARRGASTILAQSRFSLPLQT
                     LTPLALADGASYLMLLNPTGGVLGGDRLVTEIVQEAETHVCLTTPSATRIYRTLEEPA
                     ILGTVIRIGEKATLEYFPDHIIPHEGSALGQSLRVEMAPGSQAILFDSMASGRVAHGE
                     RWKFRELDSRMEVFFSGRPTYINRSRILPAVQRPDRAGWMEDFDYMGSVGFFADQFSG
                     WKELCAALNEEMNIVPNMKGAASILSRNGCMVRFLARSASDMTLANQKLWDVGRRTLL
                     RLPPFEHRKY"
     gene            5609..6406
                     /locus_tag="VG272_07685"
     CDS             5609..6406
                     /locus_tag="VG272_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13600.1"
                     /translation="MITPLAIILLGFFLGMRHAIDPDHVVAVTTIVSRERTMMNATVI
                     GALWGLGHTFTILVVGSAIILFKLTIPARIGLSMELSVGFMLILLGILNLTGILRRAM
                     EWITIRRLGAGAHPHFMFGRRMVHTHDEYGEAVSVDSFSIVGWTPQWWKQLGMFQSLR
                     PLVIGIVHGLAGSAAVALLVLTTISRPSWAIIYLLIFGLGTVAGMMLITAAIALPFAY
                     SLQRFAKLNHGLAMASGFVSIAFGLFLCYQIGFVDGLFTAHPSWTPH"
     gene            complement(6534..>6768)
                     /locus_tag="VG272_07690"
     CDS             complement(6534..>6768)
                     /locus_tag="VG272_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13601.1"
                     /translation="LLGEISRVTGGTIHPSIDQLLNDKGAIVRERQPLWPYWLVLALF
                     LNLIEVALRKGFFERLSIWARKHSPASRDRQPA"
BASE COUNT         1523 a         1814 c         1918 g         1513 t
ORIGIN      
        1 tctatgaatc tctggaagga aaggtttcga tctattacgc agctaacaaa ctccgcctcg
       61 ccgacggata aattcactca gaaggggtga catttttatc gggccgttag ggtgacattt
      121 ttatgttgct acaacagccc tgagaaggat agtgttcggc cagcagagaa ttattgccgg
      181 ggattgaatc gtggcgcaat actgagcatt tggattttgc atttccattc gctagccgct
      241 tggttatcat tcgcaggggc gaatggacgc acgcgctcac gggcacaaac taaatttgaa
      301 gggcagcaaa aaaacgcagc atgcatctca cgcctcgcga aattgagaag ctcatgatcg
      361 ccacggcagc ggaactggcg cgcaaacggc gggcgcgcgg gctgcgcctt aattatcctg
      421 aggcggtggc tttgattacc gccgatctgc tcgaagggat tcgcgatggg cgctcggtgt
      481 ccgacctgat ggaattcggg gccacaattt tgaaacgcga ggaagtgatg gaaggcgtgc
      541 cggaaatggt ggacgaagtc caggtagaag gaacttttcc agatggcacg aaactggtga
      601 cggtacatca tcccatccgc tagaaagtag cgcgggcttc agcctgcggg ggttaggact
      661 tgcaaggact gaattccgca gacggaagcc cgcgttactg aagctagatt tgcacttgag
      721 aggtgtccca tggttcccgg tgaatattta ctggcggagg gcgacataat cgccaatgcg
      781 gggcgccgcg aaatcgaatt ggaaattgct aacaccggag accgtcccat ccaggttggc
      841 tcccatttcc atttctttga agtgaatcgc gcactgcgat ttgaccgtgc cgccgctttt
      901 ggcatgcgat taaatattcc cgcaggaacc gcggtgcgat ttgagcccgg cgattccaag
      961 aaaattacgc tggtcgaaat cggcggcacg aaacatgttt acggactcaa cggattgacg
     1021 aacgggcccg cggatgaagc gaacaaaccc aacgcactga agcgcgcctc cgaccatgaa
     1081 ttttaggatt gtgcaagacg tccgcccagg gggactgtac ccaatgagaa cgcgacaaat
     1141 gaaaccatga gcctgaaaat tccccgccgc caatatgccg atctgtacgg accaaccacg
     1201 ggcgatcgcg tccggctggc cgacacggaa ttgttcatcg aaattgaaaa ggacctaacg
     1261 actcccggcg aagaagcgaa attcggcggc ggcaaagtca ttcgcgatgg catgggccag
     1321 tcttcgacgg caacttcggc ggatggcgtc cttgatttgg tcatcaccaa tgcggtgatt
     1381 gtggatcatg gcggaatcat caaggccgac atcggaatcc gcaatggacg catcgtcggg
     1441 atcggcaagg caggcaatcc ggacatcatg aacggcgtaa cgtccggcat gattattggc
     1501 gcgggaacgg aagtgatcgc gggcgaagga aaaattgtga cggccggcgg aattgacgcg
     1561 cacgtccatt ttatttgccc gcagcttgtg gagacagctc tctccggagg cctgaccacg
     1621 ctgatcggcg gcggcactgg cccggctacc ggcacggacg ccaccacatg cacgcccggc
     1681 atctggaata tgcatcgcat gctggaatcc gcagatggtt ttccgatgaa tctgggattt
     1741 ttgggcaagg ggaattcttc ttctccagag ccgctgcgcg agcaaattga agctggcgcg
     1801 attggcctga agttgcacga agactggggt acgactcccg cggccatcga tcgcgcgctc
     1861 tccgttgcgg aagagttcga cgtgcaagtg gcgattcaca cggacacgat taacgaagcg
     1921 ggctacgtgc aagattccat ccgcgcgttc aagggacgca cgattcacac gtatcacacc
     1981 gaaggcgcag ggggcgggca cgcgccggac atcatcaaag tttgcggcga actgaattgc
     2041 ctgccctcct ccacaaatcc cacgatgccg ttcacagtca acacgctcaa cgagcatctg
     2101 gacatgctca tggtgtgcca tcacctgagc ccttcgattc cggaagatgt ggcctttgcg
     2161 gaatcgcgaa tccgcgcgga aacgattgcc gccgaagatg ttcttcacga cattggcgcg
     2221 atcagcatga tgtcttcgga ttcgcaggcc atgggacgca tcggcgaagt cattacgcgc
     2281 acctggcaaa cagcggacaa aatgaaaaag cagcgcggcg ctctgcccgg agaaaaatct
     2341 ggaagcgata atattcgggt tcggcgttac gtcgcgaagt tcacaatcaa tcctgcaatt
     2401 gcacacggga tttcacagga agtcggctcg gtggaaactg gaaagctagc agaccttgtg
     2461 ctgtggaaac cggcgttttt tggcgtgaag ccggaaatga tcgttaaggg cgggatgatt
     2521 gcctgggccg cgatgggcga tgccaatgct tcgattccta cgccgcaacc cgtgatctac
     2581 cggcccatgt acggaagtta cggaggcgcg ccgcactcga cttctctgac gttcctgtcg
     2641 agggcggctg tccattccga tttgcgcgac aagcttggac tgaagaaaaa aactgtcgcc
     2701 gtgaatcatt gtcgcgggct caccaagaaa aacatgatta acaacgatgc cctgccgcgc
     2761 atcgaggtcg atgccgacac ctatgaagtt cgcgcggacg gcgtgctcct tacatgcaaa
     2821 gccgcgcagg tattgcccat ggcgcagcgt tacttccttt tctgatgtgg ggcaacacac
     2881 tcatcatcga ccacattgct gctgccgcga acccggaaaa tttaaagggg aagcaggaag
     2941 ataagcttgt ctttacgtgg gagcagcgcc actggatgcg cggccgcttt acgacggcgc
     3001 aaggacgcaa gattggtatt gcgcttccta ccggcacaat tcctgctcca ggtacgattc
     3061 tgtacgtagg agaggattgg tatctcaaaa tcgaagccgc gccggaagcc gttctggaaa
     3121 tttcttttaa agattccgcg gaagccgtaa aaattgcgtt tgaagtcggc aatctccatt
     3181 ttccgctggc gctggtggaa ggaaaaatac tggttcctga tgacaaagcg atggttcgct
     3241 taatggaacg tctcggcgta tgttacgaac gccggcaaac catttttcat cccattggga
     3301 actcgcaact gcaccaacat ggataaattt cttagccttc ttcaattttc ggacggactt
     3361 tttcccgcgg gagcctacgc gcactccttt ggccttgagg cctgcgtgca agcgggcgaa
     3421 gtcagcgatg caaatggagt cgaaagtttc ctgcgtggct atttggaagg ctgtgccgcg
     3481 cccaccgatg tcgttgccgt gctttgcgcg tggcgagcag cgaaaaccgg gagtctcaac
     3541 tcatgtctgt cgctcgacga aagactcgac gccatgaaaa cgccgagcga attgcgggac
     3601 gccagccgcc agatgggaag acaaacgctt cgcgttgcga cccatcttcc ctgccattcg
     3661 ctgctcgaag agtttgcgaa atccgtcgcc gaggaaaata caccgggaca tcacgccgta
     3721 attttcggga tcatcggcgg cgttctcgat tgggacgcaa tggatatgac cggcgcatat
     3781 ttgtattcga caagcgcggt cctggttggc gcggcgctca gactgttgcc gctcggccag
     3841 cttgccggac aacgcatcct ctggaatgtg aggccgctca tcgcgaccct tgccgcggag
     3901 gctcaaagca aaaccgagac ggatatgtgg tccttcgctc ccgcgctcga agttgcgagc
     3961 atgcggcacg cactgctcga tgcgaggttg tttcgatcgt gattacaaaa ccgcttaaaa
     4021 tcggcgttgg cggtccagtc ggttcgggaa aaaccgcgct cgtcgaagtg ctttgttcgc
     4081 gcttgaaatc ttcctaccaa ctcgcagtga ttaccaatga catttacacg cgcgaagatg
     4141 cggaatttct gacgcgccgc ggcgtgcttc cgcctgaacg aattgtcggc gttgaaaccg
     4201 gcggctgccc gcacaccgcg atccgcgaag atgcttccgt gaatctcgaa gcgtcaagcg
     4261 cactcatgcg ccggttcccg gacctggaca ttttatttat cgaaagtggc ggcgataatc
     4321 tggcggctac gttcagcccg gaattggtcg atgccacgtt ttacgtaatc gatgtggccg
     4381 agggcgaaaa aattccgcgc aagggtggac ccgggatcat gacgtctgga atgctggtga
     4441 ttaacaagat cgatctcgca ccctatgttg gcgcggatct ggcggtgatg gatcgcgatg
     4501 ccaggcgcat gcgcggagag aggccctacg tcttcaccaa tctcaagaac ggtaccggag
     4561 tcgacacact catcgattgg attcgccact ccctgctcct tgaacagtga ttttcagggc
     4621 gagcgaattc ctaaccgtgt cgggcgcgac ggatttctcc gtttacgatt cgcgcgacgc
     4681 ggcgcaagta cgatacttgc gcaatcgcgc ttctcgcttc ccttacagac gttgacgccg
     4741 cttgcccttg cggatggcgc ttcttacctg atgctgctca accccacggg cggagtcctg
     4801 ggtggcgatc gccttgtcac cgaaattgtg caggaagcgg aaacgcatgt ttgcctcacg
     4861 actccttccg cgacacgaat ttaccgaaca ttggaagagc ccgcgattct gggaacggta
     4921 attcgcattg gcgaaaaagc cactctcgaa tattttcccg accacatcat tccacatgag
     4981 ggctcggcgc taggccaatc cttgcgcgtg gaaatggcgc cgggaagcca ggcaattctc
     5041 ttcgattcca tggcctctgg tcgcgtggcg cacggtgaac gctggaagtt cagggaactg
     5101 gattcacgta tggaagtttt cttctctggc cgcccgactt acatcaaccg atcaaggatt
     5161 ctccccgcgg tgcagcggcc cgatcgtgca gggtggatgg aagatttcga ttacatggga
     5221 agcgtcggat tttttgccga tcaattttcg ggttggaaag aattgtgtgc tgcattgaat
     5281 gaagaaatga atatcgtccc aaacatgaaa ggcgcagcga gcattctttc acgcaatggc
     5341 tgtatggttc gcttccttgc ccggtccgcc tccgatatga ctctggccaa tcagaagttg
     5401 tgggatgtgg ggcgaagaac cctgctccgc cttcctccct ttgaacatag aaagtattaa
     5461 cctccgtaag ggcacatccg aataacctga ttatgcgctt aaggacgtcc gtgccaaaat
     5521 acaagtgcca ttcattgcaa accggcttag tgcggagaac aataactata gaatactcac
     5581 acttctcata cagccgctca cacacacaat gattacgccg ctcgccatta ttctcttggg
     5641 attctttctg ggcatgcgcc acgcgatcga cccagatcac gtcgtggccg taaccaccat
     5701 cgtcagccgc gagcgcacga tgatgaatgc caccgtcatc ggcgctctgt ggggattggg
     5761 tcacacgttt acgattcttg tggtgggctc ggcgattatt ttatttaaac taacgattcc
     5821 cgcgcgcatc ggcctgagca tggaattatc agtcggcttc atgctcatac tgcttggcat
     5881 tctgaatctg acgggaattt tgcgccgtgc aatggaatgg atcaccattc gtcgcctcgg
     5941 cgcgggagcg catccccatt tcatgtttgg ccgcaggatg gttcacacgc acgatgaata
     6001 tggcgaagca gtttcggtgg attcgttttc catcgtgggc tggacgccgc aatggtggaa
     6061 gcagctgggc atgtttcaat ctctgcgccc tctggtcatt ggaatcgtgc acggcctcgc
     6121 gggatcggct gccgtagcgc ttctcgtgct gacaacaatt agccgtcctt cgtgggccat
     6181 tatttatttg ctgattttcg gattgggaac cgtcgcaggt atgatgctga tcaccgcagc
     6241 cattgctctg cccttcgctt attcgctgca acgttttgcg aagctgaatc acggcctggc
     6301 gatggcgtcc gggtttgtga gcatcgcgtt cggattattt ctctgctacc aaatcggttt
     6361 cgttgacgga ttattcaccg cacacccaag ctggactccg cactgagatg tgattcgtga
     6421 attgtgattc gtaattcgtg gctggctttt agtaggacag gcttcagcct gtctgctttt
     6481 gaccttcctc acagcagaat aaatcaaaag aagacaggct gaagcctgtc ccactaagcc
     6541 ggttggcgat cccggctcgc tggcgaatgc ttgcgcgccc agattgaaag ccgctcgaaa
     6601 aatcctttgc gtagcgcgac ctctattaaa ttcaggaaga gtgcgagcac aagccagtag
     6661 ggccagagcg gctggcgttc gcgcacgatg gcgcccttgt catttaacaa ttgatcgatg
     6721 gaaggatgga tggtaccgcc tgtgacgcgg ctgatttcgc cgagcagc
//
LOCUS       DASZMB010000169        17163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_741152, whole genome shotgun sequence.
ACCESSION   DASZMB010000169 DASZMB010000000
VERSION     DASZMB010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 17163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17163
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741152"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="VG272_07695"
     CDS             <1..986
                     /locus_tag="VG272_07695"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HWF13602.1"
                     /translation="LADGEDVIIGGVMEHIEEAGVHSGDSSCVLPPVSLSPTVLAKIR
                     DYTNRLAKALQVVGLMNVQYAIQRDTVYVLEVNPRASRTVPFVSKATGVPLAKVAARL
                     MAGRKLREMNLPVVITDGVAEIAVKDYYSVKSPVFPFNKFRGVDTILGPEMRSTGEVM
                     GVAKSYGQAFAKAQLAAGQRLPRKGSIFLSVSDHDKRHCGPLAKELHALGFRLIATRG
                     TAAALEAAGVPSEAVFKVNEGRPNIVDLIKTGKVDMIINTPLGRESFYDEKSIRRAAI
                     RYNIPCITTLSAANAAARGIRALVEQTLEVAPLQSLHSKKTAAPQLPSGTD"
     gene            1035..1943
                     /locus_tag="VG272_07700"
     CDS             1035..1943
                     /locus_tag="VG272_07700"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HWF13603.1"
                     /translation="MATKPPLKGVLPALTTPFESDGGVALGRMRENVEAYNRVGLSGY
                     LAVGSTGESVLLSRGEFEGVLSTVRDAAAPGRILIAGSGTETTAETISRTEFAAKLGY
                     DYALVGPPTFFKPTLTSDVLVGHYTRVADAARIPILLYSVPQYTGVPIEVDVAARMSQ
                     HPNVAGMKDSSGNVDRIGAILAAVPETFRLLTGSSTTISSSMVMGAVGAILALADFLP
                     ELSVALYDAIVAGDANKALDLQRRLIPPTRRIVGAMGVAGVKYAMDKRGYYGGPVREP
                     LMPLSEARKKEADSLLATLVRSAVSA"
     gene            2028..3650
                     /gene="bshC"
                     /locus_tag="VG272_07705"
     CDS             2028..3650
                     /gene="bshC"
                     /locus_tag="VG272_07705"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HWF13604.1"
                     /translation="MDCSCISPADLPGTTRLYSTFLSDFSSVSNFYRHPPTAAGIDEA
                     VREVRMEDSVRRAVVEVLRKQNRTFGGGERTAENLGRLRDGAVAVVTGQQVGLFGGPA
                     YCIYKALTAIHVARELTGRGMDAVPVFWLATEDHDFAEVNHCFVPKRGGLQRLELLPS
                     GPSGRRVGDVRLGGEIRALSSQLDSLLEGAWASEIARWISESYTPEDTFGSAYGKLMT
                     RIFSGRGLIFLDPMSPELHRISIPVMKRALTEHKELAAELMARSAALEKSGFHVQVKV
                     TEQSTLLFRIVKGQRIAIRPVKDGFAAGDKTESMQETLKALESHPGDFSPSAILRPVV
                     QDTLLPTVAYVGGAAEVAYQAQTSLVYEKLLGRAPAVLPRASFTLVPSHIANLLKKYN
                     LDAREILQGRHKLRARLEAESLPEALTTKFEEGEQAINNLLESLREPLKKLDQTLLGA
                     LDTASEKMHYQFHGLRTKAGRAEGFRTGVIHTHENEIISSLLPNNSLQERAFGLLTFL
                     ASEGPGLLDFLDSHIKAGAGEHGFLFLQPSAK"
     gene            3738..4754
                     /locus_tag="VG272_07710"
     CDS             3738..4754
                     /locus_tag="VG272_07710"
                     /inference="COORDINATES: protein motif:HMM:NF009498.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
                     /protein_id="HWF13605.1"
                     /translation="MKVTPGKRKGLEAVSDSRGVIAAAAMDQRGSLMSAIAKDKGIDK
                     KAVTKEMMSEFKEAVSRVLTPHATAILLDPEYGLSAAKQRAKNAGLLLAYENSGYDNT
                     QPGRLPDLLDHYSVRRLVADGADCIKILLYYTPFDSEKINDTKHAWVERIGAECVAAD
                     VPFFLEFVTYEEGVDEKGLEFAKKKPEAVTRSMEEFSKPQYGVDVLKVEVPINMAFVK
                     GARSCKGESVYTRDEAKEHFRKSAAAAKKPFIYLSAGVSNDTFSETLELASETGVNFS
                     GVLCGRATWKDAIPVYGKHGAKALEDWLLDQGVKNINNVNDRLKAAKPWFAFYGVTSS
                     SALA"
     gene            4786..5223
                     /locus_tag="VG272_07715"
     CDS             4786..5223
                     /locus_tag="VG272_07715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HWF13606.1"
                     /translation="MNSKGKASEGRSIETLRIKTSRRTEWVNITSQVSRLVSASGISS
                     GVCRIYVSHTTAGVTINEGYDPEVASDMEAAFDRMVPRDAGYNHAEGNSDSHIKATLV
                     GSSQNVWIEEGHLQLGRWQAIFFCEFDGPHNREIQVKIVSDPT"
     gene            5272..6432
                     /gene="hemW"
                     /locus_tag="VG272_07720"
     CDS             5272..6432
                     /gene="hemW"
                     /locus_tag="VG272_07720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HWF13607.1"
                     /translation="MTRLGVYVQVPFCQTKCTYCNFHTGVFSKDLYDPYVRAVCREIS
                     DFRRARAENGIAEVSQLQVDTVYFGGGTPSLLESAQLGQIIDTIRDSFPAKFEEVTLE
                     ADPETITPEKAAAWLVVGFNRISMGVQSFNDEELRPAGRMHRRADVFEATKILRAAGF
                     SNLSFDLIAGLPHQTDASWQDSVEQLLSLRPEHVSIYLMEIDEASRLGLEVLQSGERY
                     SAKSIPSDDSMADYYESGCRQLAAAGYAHYEISNWGLPGCESRHNLKYWRREPYYGFG
                     AGAHSFNGSQRWANAHDPAAYSNAIHAGRFPIEQLESVGQGQALEEELFLGLRQLAGI
                     DLGAVEAKYSTSLQSRVGELLAQGLVEWDGPRLRLVPARLSVSNEVFVHLLE"
     gene            6404..6613
                     /locus_tag="VG272_07725"
     CDS             6404..6613
                     /locus_tag="VG272_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HWF13608.1"
                     /translation="MKYLFISWNNVAGAELAKMLAMGASRPWPDAVEVLTGQRQMDAT
                     ALMDYYAPLKKRLDEQNVGHKVGWN"
     gene            6799..7947
                     /locus_tag="VG272_07730"
     CDS             6799..7947
                     /locus_tag="VG272_07730"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HWF13609.1"
                     /translation="MDFSFTDEQRQLRQTVREFAQAEIAPHVMEWDEASKFPSELIAK
                     LAEMGLMGVIFPEEYGGAGLGYIEYVSVIEELARVDGSVGLIVAAHNSLCTNHIFKFG
                     TPEQRKKYVTPLAQGQKIGCWSLTEPQAGSDAGGARTTAVHKNGGWMLNGSKTFITNG
                     HYADICVAMAVTDKPRGTKGISALIVEKGNPGFKAGKKENKLGMRASDTSEIIFNDCF
                     VPDANLLGKEGLGFVNTLQVLDGGRISIAALALGMAQGAYEAAVGYAKQRKQFGKAIS
                     EFQAIQFKLADMATEIDAARWLVYRAAWLADQKGPRFTKESSMAKLYASEVGVRVANE
                     AVQIFGGYGFVKDYPVEKFYRDVKLCTIGEGTSEIQRMVIARQILDAR"
     gene            8113..9081
                     /gene="meaB"
                     /locus_tag="VG272_07735"
     CDS             8113..9081
                     /gene="meaB"
                     /locus_tag="VG272_07735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HWF13610.1"
                     /translation="MSVSIETWAEQIKAGDVRAVSRAITAIEDHDPRAEELLQRIFPN
                     TGKSFVIGITGAPGTGKSTLVDRLAAHYRAQQNHVGIIAVDPTSPYTGGAILGDRIRM
                     QGHASDPGIFIRSMATRGFLGGLARATGDVALVLDAAGKQYVLIETVGVGQDEIDIVR
                     LADCTMVLLVPGMGDDIQNMKAGLMEIADIFVLNKSDRDGMDRLEAELEAMLHLAPER
                     NGWKPMIVRTVATENKGVDELALAIAQYRESSDSSAERQARKIDHWKARLLTLAGESF
                     LKRAVSGEDGEAALNHLARQVANRSKDPYAAVRELLTRADDARSRA"
     gene            9078..9920
                     /locus_tag="VG272_07740"
     CDS             9078..9920
                     /locus_tag="VG272_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWF13611.1"
                     /translation="MKLGDLEFWLITDGTFRLDGGAMFGVIPKPMWEKVAPADSRNRI
                     LMAMNSLLIRAAGKNILIETGAGDKWDAKRTEIYSFDGPPRLPEKLLTHDVPLEEIDI
                     VINTHLHFDHCGWNTRIVNGEVIPLFPNARYYVQRGELEHAKNPTDRDRASYFLENFA
                     PMEKSGQWNLLEKDAEIVPGVEVIRAPGHNADMMCVRLTGGGKTVFFTADLIPTAAHV
                     PYPWIMGYDLYPLTTLENKKKWISQAVREEWLVIFGHEARTPAGYLRERQGKFELEPA
                     MIDV"
     gene            9954..10871
                     /gene="mtnP"
                     /locus_tag="VG272_07745"
     CDS             9954..10871
                     /gene="mtnP"
                     /locus_tag="VG272_07745"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HWF13612.1"
                     /translation="MAKKSQAAKRRSASAEPVLIGIIGGSGLYNMPGLEKPREVRIKT
                     PFGNPSDALVIGTLEGKRVAFLSRHARGHRYTPTEINYRANIHAMKQLGAERIISVSA
                     VGSLQEELKPLDFLIPDQFFDRTRHRVSTYFGDGVVAHVGFGNPVCTQLAETLGGACD
                     SAKVRVHRGGTYICMEGPQFSTLAESNVYRQLNFQVIGMTNLTEAKLAREAEICYSTV
                     AMITDYDCWHQGHASVTLEEILSNLSKNAENAQNVIREAVRDMPAERTCKCGRALAHA
                     IVTDPAMVPAAARKRLSLIAGKYLTLGAR"
     gene            10874..11782
                     /locus_tag="VG272_07750"
     CDS             10874..11782
                     /locus_tag="VG272_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWF13613.1"
                     /translation="MSLLVVGSVAFDAVETPHGKVDRMLGGAATYFSVAASYFTHVNL
                     VGIVGTDFTKKDESVFKGRHIDTQGLERADGKTFFWAGKYSENMNERTTLATELNVFA
                     DFKPRLPEAYTSSTHIFLANIDPTLQASVLHQVPRRPRVVALDTMNYWIERTPKELRE
                     TLKYTDILMINDSETRQLSSEHNLLVAARHIFKLGPKALVIKRGEYGAMLVHQNRTFN
                     VPAFPLEVVHDPTGAGDSFAGGFMGYLATAKKVDDAALRRAMVYGSVMGSFAVEKFGL
                     DRLRALKKSEISARARHFHKMTHFSL"
     gene            12051..12938
                     /locus_tag="VG272_07755"
     CDS             12051..12938
                     /locus_tag="VG272_07755"
                     /inference="COORDINATES: protein motif:HMM:NF036772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HWF13614.1"
                     /translation="MVAPASSTKEERIQRGCETLESLDFRVKRYSSKHKPMDFFSAPL
                     AERRKQLQDALMRPEIAAVLCSRGGYGSTEILDGLNTARLKQPKIFCAFSDLTSVHIY
                     LWQKLRWVTFYGPLVAGGFDAGPNATNGFDPDTFMWAMTATNSGWSVPLEGETFSRGI
                     AEGTLLGGCITLIETSIGTPWELDTRGSILLLEDRGVKPYHLDRMLMHLKQAGKFEGV
                     RGIILGEFPDSETPEGSSMSVAEVCKRLLGPLHIPIVYGVAVGHTQRPMLTMPLGVRA
                     KLHATGEGRIDILEPAVRA"
     gene            12935..14368
                     /gene="mpl"
                     /locus_tag="VG272_07760"
     CDS             12935..14368
                     /gene="mpl"
                     /locus_tag="VG272_07760"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: protein motif:HMM:TIGR01081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HWF13615.1"
                     /translation="MTRPLHFHLSGIGGAAMTPLAGMLAASGNRVTGSDAGVYPPAST
                     LLESLGINWKNGFDAANLDPAPDIVVIGNALSRGNPEVEAVLDNKIPYRSLPQTLEEY
                     FLPGHDSLVVTGTHGKTTTTSMLSWILHHAGRHPNFLIGGVAENFGRSFGLGGGKEFV
                     LEGDEYDSAFFDKAPKFLHYHPQEMILTSLEFDHADIYENLAAIELQFRRLVNLVPKS
                     GRIVAWGESETVRGVLQKAYCTVETYGFAPGVDWLAGDLEIRDGETHFRVSRSGKEVA
                     RVKMALAGRHNVLNATAAVAIAHGRGVSGEAIAGAMAEFRGVLRRLEIKGEPSGVLVV
                     DDFAHHPTAIRATIDAARHRWPGRKLWAVFEPRSNTMRRKVFEKDLADSLATADAAVL
                     GAVNRANLLSDAERMSPARVLDTITGAGHSAAGFDSVDEIAEYLASETRPGDLVLVMS
                     NGSFDGLCGKLLGKLQERGAPAGKSRA"
     gene            14365..16164
                     /locus_tag="VG272_07765"
     CDS             14365..16164
                     /locus_tag="VG272_07765"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWF13616.1"
                     /translation="MMFRLRPWSCAAILAAIFYFSSPAHATIEYTVSLTHPERHVFNV
                     AMRIPNVRDHLTVQMPAWNALYQIRDFSSHIMQASAKDQAGKPLPMAKLDKQTWSVSA
                     SGTVTVTYGIFWDESGPFASQLNSEHAFINSAMLLLYVPDRRAEDTKIVFDDVPEGWR
                     VAVELEAAAVSAGHHAGAYVAPSYDALVDAPVEIGHFEEFHTEAGGRPIRIVVHGDAG
                     DHSKLVESLKKIVDYDVTLMGGAPFREYMFLFHVGNNFGGGGMEHMNCTAIAADFPAQ
                     LPSYSAHEFFHAWNVKRIRPQALEPVDYTKEMYTRALWFAEGVTNTYGSYALERSGLW
                     SAQQFYANLADQIRELQSRPAHRWQSVEQSSLDSWFEKYPLYNRPEESISYYNKGQIL
                     GVLLDIVIRDRTDNHASMDDVLRALNEQYAKAGRFYNESDGLRAIMEEVIRKKDPGAI
                     PDLSDFFTRYVAGTDEIPYSDFLSFAGLALHESQRRAAFGFAISHDTASNVTIASVDR
                     DSGAGEAGLKEGDVLLQLNGESFPRIPERWLRDHQPDERVTVKIKRGGEVLDISFPLG
                     RVTDSTFQITEIASPTERQRRIRNGILRGITTP"
     gene            16166..16882
                     /locus_tag="VG272_07770"
     CDS             16166..16882
                     /locus_tag="VG272_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWF13617.1"
                     /translation="MIRSAFVAIAMSLCTLVLGPPLILLTLITKSATLMYWTGVKMVV
                     YVTRIAGEKVTVEGVENIPPGVCLFAANHTSNADAPAIVGAIPRRIAILGRKSLFDIP
                     VVGMAFRLAKFVPVDRGNRDAALESVKIAVQYIHEGFSFLVYPEGTRSPDGRLQRFKK
                     GSFAIAIEAGIPIVPVACAGAHRIMKKHSLIVRPGKVTVQFGKPIDASKFTIEQRHEL
                     AQTVYEAIAADLPEDQKPMQ"
BASE COUNT         3884 a         4441 c         4987 g         3851 t
ORIGIN      
        1 cgctggccga cggggaggac gtgattattg gcggcgtaat ggaacacatc gaagaggcgg
       61 gcgtgcactc gggagattct tcgtgcgtgt tgcctcctgt cagcctgtcg ccgacggtgc
      121 tggcgaagat tcgcgattac accaaccggc tggccaaggc cctgcaagtt gtgggcctga
      181 tgaatgtgca atacgcgatt caacgcgaca cagtctatgt gctggaagta aatccgcgcg
      241 cctcgcgcac ggtgcctttt gtcagcaagg cgacgggcgt gccgctggca aaagttgcag
      301 cgagattgat ggcggggcgc aagctgcgcg aaatgaattt gccggtcgtg attacggacg
      361 gcgtagcgga aattgcagtg aaggattatt actccgtaaa atcgcccgtg tttccgttca
      421 acaagttccg cggcgtggac acgattcttg gtcccgagat gcgttccacc ggcgaagtga
      481 tgggcgtcgc caagagttac ggccaggcat ttgccaaggc gcagctcgcg gcgggacagc
      541 gattgccgcg caaaggctcc atctttttga gcgtgagcga tcatgacaag cgccactgcg
      601 gacctcttgc gaaagaattg cacgcattgg gattccggtt gattgcgacg cgcggcacgg
      661 cagcggcgct cgaagcggca ggcgttccgt ctgaagcagt tttcaaggtg aacgagggcc
      721 gtccgaacat cgtcgacctg atcaaaaccg gcaaagtgga catgatcatc aacacaccgc
      781 tgggccgcga atcgttttac gatgagaaat cgattcgccg cgctgcgatc cgctacaata
      841 ttccttgcat cacaacgctc tccgccgcaa atgccgccgc tcgcgggatt cgcgcgctgg
      901 tcgagcagac gctggaagtt gctccgctgc aatccttgca ctccaagaaa accgcggcgc
      961 cgcaactgcc gtccggaaca gattgatgcc ggaccgttcg tcaaaaattt agttccagcg
     1021 cgaggagaac acacttggca accaaaccac cactgaaggg agttttgccc gcccttacga
     1081 caccgtttga gtccgacggt ggagtggctc tcggccggat gcgggaaaat gtcgaagcct
     1141 ataaccgggt tggtttatcc ggatatttgg ccgtaggttc gaccggcgaa tctgttttgc
     1201 tcagtcgcgg cgaatttgag ggcgttctct caaccgtgcg cgatgccgcc gctccgggac
     1261 gaattttaat tgccggatcg ggaaccgaga caacggcaga aaccatttcg cgcaccgaat
     1321 ttgcggcaaa gcttggctac gattacgcgc ttgtcggtcc gcccacattt ttcaagccta
     1381 cgctgacctc ggatgttctg gtaggccatt acacccgtgt ggcggacgct gcacgaattc
     1441 ccatcctgct gtattccgtg ccgcaataca caggcgtgcc aattgaagtg gatgtcgcgg
     1501 cgcgcatgtc tcagcacccg aacgtagcag gaatgaaaga tagttccgga aacgtggatc
     1561 gcatcggcgc aattcttgct gccgtgccag aaacgtttcg cctgctgacc gggtcttcca
     1621 caactatttc ttcgtccatg gtgatgggag cggtgggcgc aattttggca ttggctgatt
     1681 ttctgcctga actcagcgtt gcactttatg atgcgatcgt cgccggagat gcgaacaagg
     1741 cgctcgatct gcaacgcaga ctaattccgc ccacgaggcg cattgtcgga gcgatgggtg
     1801 ttgccggcgt gaagtacgcc atggacaagc gcggttacta cggcggaccg gttcgcgagc
     1861 cactgatgcc tctgagtgag gcgcgaaaga aggaagccga tagtctactc gcaacgctcg
     1921 tgcgttcggc tgtttcggcc tgaatcctaa cgaatccagg gtcatctaat gagctttgcg
     1981 aaggagctcg ccggtcacgc tgtgcaagta caaggatgga atgcctgatg gattgtagtt
     2041 gtatctcacc ggccgatttg cctggaacca cgcgcctgta ttccacattt ctttccgatt
     2101 tttccagcgt ttccaatttt tatcgccatc ctcccactgc cgcgggaatc gatgaagctg
     2161 tccgcgaagt tcgtatggaa gattctgttc gccgcgccgt tgtcgaggtg ctccggaaac
     2221 agaatcggac ttttgggggc ggtgaacgaa ccgctgaaaa tttgggtcga ttgcgagatg
     2281 gcgccgtcgc ggtggttacc gggcagcagg ttgggttgtt cggaggtccc gcgtattgca
     2341 tttataaagc gctgaccgcc attcacgtag cgcgcgaatt gaccggtcgc ggcatggatg
     2401 ctgtaccagt tttttggttg gcgaccgaag atcatgattt cgccgaagtg aatcactgct
     2461 ttgtgccgaa acgcggcggc ttgcagcgtt tagagttgct gccctctggc cctagcggcc
     2521 gcagagtcgg cgatgttcga ttgggcggcg aaattcgtgc gctatcgtct caattggatt
     2581 ctcttctcga aggtgcatgg gcttcggaaa tcgcgcgctg gatatccgaa agctatacgc
     2641 ccgaggatac gttcggttcc gcgtacggaa aactgatgac gcgcattttt tccgggcggg
     2701 gcctgatttt tctcgatccc atgtcgccag agttgcatcg catttccatt cccgtcatga
     2761 agcgcgcgct cacggaacat aaagaattgg ctgcggaact catggcgcgc tcggcggcgc
     2821 ttgaaaagtc tgggttccac gtgcaggtaa aagtcaccga gcaaagcacg cttctgtttc
     2881 ggattgtaaa agggcagcgt atcgctattc gccccgtgaa agacggtttt gccgccggag
     2941 acaagaccga atcgatgcaa gaaacgctaa aagcattgga atcgcacccg ggagatttca
     3001 gccccagcgc aattctgcgt ccagtggttc aggatacgct gctgcctacg gtcgcctacg
     3061 tcggcggagc ggctgaggtc gcgtaccagg cgcaaacttc gctcgtgtat gaaaagttat
     3121 tgggcagagc gcccgcggtt ttgccgcgcg cgagttttac gctggtgccg tcccacattg
     3181 ccaacttgct gaagaaatat aatctggatg cccgcgaaat tctgcaaggg cgccacaaac
     3241 ttcgggcacg cctcgaagcg gaatcactgc cggaagcact gacaacgaaa tttgaagagg
     3301 gcgagcaggc gattaacaat ctgctcgaaa gcttgcgcga accgcttaag aaactggatc
     3361 agacattgct cggcgcgctc gataccgcgt ccgagaaaat gcattatcag ttccatggcc
     3421 tgcgaaccaa ggcgggccgc gccgaaggtt ttcgcacggg cgtaatacat acacatgaga
     3481 acgagattat ctcttcactt ctgccgaata actcgcttca ggagcgggcc ttcggcttgc
     3541 tcacatttct agcctctgaa ggtccgggcc tgctggattt tctcgatagc cacattaaag
     3601 ccggagcagg cgagcacggt ttcttgtttc tgcaaccaag cgcgaagtag agtttaagcc
     3661 ccatgtattt ttggctgcaa acacactaga atgcaagaag tggttattct tgtagcttat
     3721 gagcggagga ttttctgatg aaggttactc ctggaaagcg caagggactc gaagcggttt
     3781 cggattcgcg cggcgtgatt gccgctgcgg ccatggatca gcgcggatcg cttatgagcg
     3841 cgattgccaa ggataaaggc atcgacaaga aagccgttac gaaagaaatg atgtcggaat
     3901 tcaaggaagc ggtgtcacga gtcctgacgc cgcacgcgac ggccattttg ctcgatccag
     3961 aatacggtct gtccgcagcg aaacagcgcg cgaaaaatgc gggcctgctg cttgcctacg
     4021 aaaacagcgg ctacgacaac acgcagcccg gacgtcttcc cgatttgctg gatcattatt
     4081 ccgtgcgtcg cctggtggcc gatggcgccg attgcatcaa aattcttttg tattacacgc
     4141 catttgattc cgagaaaatt aacgatacca agcacgcgtg ggtcgagcgt atcggcgcgg
     4201 aatgcgttgc cgccgacgtt ccatttttcc tcgaatttgt gacctacgaa gagggcgtgg
     4261 acgagaaggg tctggagttt gcgaaaaaaa agccggaagc cgttacgcgc agcatggaag
     4321 aattcagcaa gccgcagtac ggcgtggatg tattgaaggt cgaagttccc atcaatatgg
     4381 cgtttgtcaa gggcgcgcgc tcctgcaagg gagaatccgt atacacgcgc gacgaagcga
     4441 aagaacattt ccgcaaatcc gccgctgccg cgaaaaagcc gttcatttat ctatcggcgg
     4501 gcgtgagcaa cgatacattt tccgaaaccc tcgaactagc ctctgaaacg ggtgtgaatt
     4561 tttcaggcgt tctgtgcggt cgcgcaacct ggaaagatgc aattccggtt tatggcaagc
     4621 atggcgcaaa agccctggaa gattggctgc tggatcaggg cgtgaaaaac atcaacaacg
     4681 tgaatgaccg cctgaaggcc gccaagccgt ggttcgcgtt ctacggcgta acgtcgtcat
     4741 cagccctcgc ataaatctca ttcgataatt cctgattgta ggttcatgaa ttccaaaggc
     4801 aaagcttcgg aaggccggtc cattgaaacg ctgcgcataa aaacatcgcg gcggacggag
     4861 tgggtgaata ttacttctca agtgagtcga ttggtgtcgg cctccggaat ttcatctggc
     4921 gtttgccgga tttatgtttc gcacaccacc gctggagtaa cgatcaacga aggatacgat
     4981 cccgaggttg ccagcgacat ggaggccgcg tttgaccgca tggttccgcg cgacgccgga
     5041 tacaatcatg ccgaaggaaa ttccgattcg catatcaagg cgacgctggt gggatcttct
     5101 caaaatgtat ggattgaaga gggccatctt caactcgggc gctggcaagc cattttcttc
     5161 tgtgaatttg acggtccgca caatcgcgaa atccaagtga aaattgtttc ggatcccaca
     5221 taaagccatc cggcgcaata cttcagaaat tcatgttaag gtctaggaat catgacgcgg
     5281 ctcggcgtat acgtgcaggt tcctttttgc cagacgaagt gtacctactg caacttccat
     5341 accggcgtat tttctaaaga tctttacgat ccatacgtgc gagcggtttg ccgtgagatt
     5401 agcgattttc ggcgtgcgcg agctgaaaat ggaatagcag aagtctcgca actgcaggtc
     5461 gacactgtat atttcggtgg tggaactccc agcctgctgg agtctgcgca actcggccag
     5521 atcatcgata cgattcgcga ttctttcccg gcaaaatttg aggaagtgac tctcgaagcc
     5581 gaccctgaaa ctattactcc ggaaaaagct gctgcatggc tcgttgtcgg attcaaccgc
     5641 atcagcatgg gcgtgcagtc ctttaacgat gaagaattgc gtcccgcggg acgcatgcat
     5701 cgccgcgcgg acgtgtttga ggcaacgaaa atcctgcgcg ccgccggatt cagcaacctc
     5761 agcttcgatc tgatcgcggg gcttcctcat cagaccgatg caagttggca agattcagtc
     5821 gagcaactgc tgagtctgcg tccggagcac gtctcgattt atctgatgga aattgatgaa
     5881 gcaagccgcc tgggcctcga agttctgcag agcggtgagc gctactccgc aaagtcaatt
     5941 ccatcggacg attcgatggc cgattattac gaatcaggct gccgacaact tgccgccgcc
     6001 gggtacgcgc attacgagat ttcgaattgg gggttgccgg gatgcgaatc gcgtcacaac
     6061 ctgaaatatt ggcggcgcga accctattac ggattcggcg cgggcgcaca ttcctttaac
     6121 ggttcgcagc gctgggccaa tgctcatgat ccggcggcgt attcgaacgc cattcacgca
     6181 ggaagatttc ccatcgagca attagaaagt gttgggcagg ggcaagcgct cgaggaagag
     6241 ctgttcttgg gcttgcggca gcttgctgga atcgatctgg gcgcggttga ggcaaagtat
     6301 agtacgtctc tgcaatctcg cgtaggagag cttctggcgc aaggcttggt cgaatgggat
     6361 gggccgcgcc tgcgtcttgt tccggcacgg ctatctgttt cgaatgaagt atttgttcat
     6421 ctcctggaat aacgtggcgg gcgcggaact ggcgaaaatg ttggcgatgg gcgcgagccg
     6481 cccatggccc gatgcagtgg aagttttaac cggccaacgc caaatggatg cgacggcgct
     6541 gatggattat tacgcccctc taaaaaaaag gctcgacgag caaaacgtag gtcacaaagt
     6601 aggatggaac tgaagtttgc gtggcatagg cttcagcctg tggaattttg cccttgcaag
     6661 gcctgaaccc cacaggctaa agcctatgcc acggttcccg cacatggaaa ttctctccgg
     6721 ttaagcaaaa agtcctgcac attgcatgtg atataaatag gttcaggggc ttatattgtc
     6781 gcggcctcgt gaggacctgt ggatttttcc tttaccgacg agcaaaggca actccgccaa
     6841 acggttcgcg aattcgccca agcggaaatc gcgccgcacg tgatggaatg ggacgaagcc
     6901 tccaaatttc cttccgaatt gatagccaag ctcgctgaaa tgggtctgat gggagtgatt
     6961 ttccccgagg aatacggcgg cgctggtctt ggatacatcg aatatgtttc agtaatcgaa
     7021 gaacttgctc gcgtcgacgg gtctgtagga ttaatcgtcg cggcgcacaa ttcgctgtgc
     7081 accaatcaca tctttaaatt tggaacacca gagcagcgca aaaaatatgt aacgccattg
     7141 gcccaaggcc aaaagattgg ttgctggtcg ctgaccgagc cgcaagcggg ttcggacgcg
     7201 ggcggggctc gcaccactgc tgtgcacaag aacggcggct ggatgctcaa cgggtcaaaa
     7261 acattcatca ccaatggtca ctacgcggat atttgcgtcg cgatggccgt gaccgacaag
     7321 ccccgaggaa cgaagggcat ctccgcgctg atcgtggaaa aagggaatcc aggatttaag
     7381 gccggtaaaa aagaaaataa gctgggcatg cgcgcaagcg acacctctga aattattttc
     7441 aacgattgtt ttgtgccgga cgccaatcta ttgggcaagg aaggcctcgg gttcgtcaac
     7501 actttgcagg tgctcgatgg aggccgcatt tcgattgccg cgctggcgct ggggatggca
     7561 cagggcgcgt acgaggctgc ggtcggctac gcgaagcaaa gaaagcaatt cggaaaggcg
     7621 atcagcgagt ttcaggccat tcaattcaag ctggctgaca tggctacgga aattgacgcc
     7681 gcgcgctggc tggtttatcg agcggcctgg cttgccgacc aaaaaggtcc gcgcttcacg
     7741 aaagaatcca gtatggccaa attgtatgct agtgaagtcg gcgtgcgcgt ggcgaacgag
     7801 gccgtccaaa tctttggcgg gtacggattt gtgaaggact atcccgttga gaaattttac
     7861 cgggacgtta aactctgtac catcggagaa ggcacgagtg aaattcagcg catggtgatt
     7921 gcgcggcaaa ttctggatgc gcgctgattc tgtccccgcg aattctgtag atgctagttc
     7981 gtgtttttga atgcagtctt cactttggtt gtgttaactt cgttattccg ctccagtcgg
     8041 atttttttaa cgcgtacatc tgaattcgta aaccggcgag ggtcttgtgg cgccggcgcc
     8101 acgaaaatca aaatgtcagt atcgattgaa acctgggctg agcaaattaa ggcgggagac
     8161 gtgcgtgcgg tttcgcgcgc cattactgcg atcgaagacc atgacccgcg tgccgaagaa
     8221 ctcttgcagc gaatttttcc taacacgggg aaatcgtttg taatcgggat tacgggcgca
     8281 cccggaacgg ggaaaagcac tctggtcgat cgactggctg cgcattaccg agcgcagcaa
     8341 aatcacgttg ggattatcgc tgtcgatccg accagccctt acacgggcgg cgcaattctg
     8401 ggcgatcgta ttcgtatgca ggggcacgca agcgatccgg gaatttttat tcgctcgatg
     8461 gcgacgcgcg gatttcttgg aggcctcgcg cgcgccacgg gtgatgttgc gctggttctg
     8521 gacgcggcag ggaagcagta cgttctgatt gaaaccgtcg gcgtcgggca ggacgaaatt
     8581 gatattgtgc gcctcgcgga ctgcacgatg gtcttgcttg tgcctggcat gggcgacgac
     8641 attcaaaata tgaaggccgg gctgatggag atcgcggata tttttgtgct gaataaatcc
     8701 gatcgcgatg gcatggatcg cctggaagcg gaactcgaag caatgctgca tctcgcccct
     8761 gagcgcaatg gatggaagcc aatgatcgtt cgcaccgtgg ccacggaaaa caaaggtgtg
     8821 gacgaacttg cgctggcaat tgcccaatat cgcgaatctt ccgatagcag cgcggaacgt
     8881 caagcacgga aaatcgatca ttggaaagcg aggcttctga cgcttgccgg ggaatcgttt
     8941 ttaaaacgcg ccgtatccgg cgaagatggc gaagccgcgc tcaatcattt ggcgcgtcaa
     9001 gtagcaaatc gcagcaaaga tccttatgca gccgttcggg aattattgac gcgcgcggac
     9061 gacgcacgga gcagagcatg aaacttggtg acctggaatt ttggctgatc acagacggta
     9121 ctttccggct ggacggtggc gcgatgttcg gcgtaatccc caagccgatg tgggagaaag
     9181 ttgcgccggc cgactcgcgc aatcgaatcc tgatggccat gaattcgctg ctgattcgcg
     9241 cggcgggtaa aaatattctg atcgaaacag gggcgggaga taagtgggac gccaagcgca
     9301 cggaaattta ttcgttcgat gggccgccgc gtctgcctga gaaacttctg acgcatgacg
     9361 ttccccttga agagatcgac atcgtcatta acactcatct tcacttcgat cattgcgggt
     9421 ggaatacacg cattgtaaat ggcgaggtga ttccactttt tccaaacgct cgatactacg
     9481 tgcagcgcgg cgaactggaa cacgcgaaga atcccacgga ccgcgatcgc gccagctatt
     9541 tcctggaaaa ctttgcaccg atggaaaaat ccgggcagtg gaatctgttg gagaaagacg
     9601 cggaaatcgt tcccggcgtg gaagtcatcc gtgcgccggg ccataacgcg gacatgatgt
     9661 gcgtgcgatt gacgggcggc gggaaaacag ttttcttcac tgctgactta atccctacgg
     9721 cggcgcacgt cccttatccg tggatcatgg gatacgattt gtatccgtta acgacgctgg
     9781 aaaataaaaa gaaatggatc tcgcaggccg ttcgcgagga atggctcgtc attttcgggc
     9841 acgaagcaag aacacccgcg gggtacttgc gcgaacgcca gggaaaattc gaacttgaac
     9901 cggcgatgat cgacgtctaa attcttttcg acgctttacg agaggtcacg cgaatggcta
     9961 agaaatctca ggcagcaaag cggagaagtg cgagcgcgga acctgtcctt atcggcatta
    10021 tcggtggtag cggcctttac aatatgccgg ggttggaaaa gccgcgcgaa gtgcgcatca
    10081 aaacgccttt tggaaatccg tcggatgcac ttgtcattgg cacgctcgaa ggaaaacgag
    10141 tggcatttct atcccgccat gcgcgcgggc atcgctacac tcccacggag atcaactacc
    10201 gcgccaatat tcatgcgatg aaacaattgg gcgcggagag aattatttcg gtgagcgcgg
    10261 ttggttcgct gcaggaagag ttgaaaccgc ttgattttct gattccggat caatttttcg
    10321 atcgcacgcg gcaccgggtt tccacatatt ttggagacgg cgtcgtcgcg catgtcggtt
    10381 ttggcaatcc ggtctgcacg cagctagccg aaactcttgg cggcgcgtgc gattcggcga
    10441 aggtgcgcgt gcaccgtggc ggcacttata tctgcatgga agggccgcaa ttctccacgc
    10501 tggccgaatc gaatgtatat cgccaattga attttcaagt gattggcatg accaacctga
    10561 ccgaagccaa actcgcacgc gaagctgaga tttgctactc cacggtggcg atgatcacag
    10621 actacgattg ctggcaccaa ggccacgctt ccgtcacgct cgaagagatt ctctccaact
    10681 tgagcaagaa cgccgagaac gcgcagaacg tgattcgcga agccgtgcgc gatatgcccg
    10741 ctgagcgcac atgcaaatgc ggacgtgcgc tggctcatgc aattgttacg gaccctgcca
    10801 tggttcctgc cgcggcgcgc aagcgcctga gcctgatcgc cggaaaatat ctcactctgg
    10861 gagcccgctg aaaatgtcgc tacttgtcgt aggatcggtt gcgttcgatg ccgtggaaac
    10921 tccgcacggc aaggtggacc gcatgctcgg cggtgcggcc acatattttt ccgttgctgc
    10981 aagttatttc acgcatgtca atctggtggg cattgtgggc acggatttta cgaagaagga
    11041 cgaatcggtt tttaaaggcc gccacatcga tacgcagggc ctcgagcgcg cggatgggaa
    11101 aacttttttc tgggcgggga agtatagcga gaacatgaat gagcgcacca cgctggcgac
    11161 ggaattgaat gtgttcgccg acttcaagcc gcgcctgcct gaggcctaca cctcttcgac
    11221 gcacattttc ctggcaaaca ttgaccccac gctgcaagct tcggttttac accaggtccc
    11281 gcgccggccg cgcgttgttg cgctggacac gatgaattat tggattgaac gcacgccgaa
    11341 ggagttgcgc gaaacattga agtacaccga catcctgatg attaacgact cggaaacgcg
    11401 ccaactctcg agcgagcata atttattggt ggcggcacgg cacattttca agctagggcc
    11461 aaaagcgttg gtcattaagc gcggagaata cggcgcgatg ctggtgcacc aaaatcgaac
    11521 ctttaatgtg cccgcgtttc ctctggaagt ggttcacgat ccaacaggtg cgggagattc
    11581 gtttgctggt ggattcatgg gctatctcgc aacggcaaaa aaagtagatg atgcggcgct
    11641 gcggcgcgca atggtttatg ggtcggtaat gggcagcttt gcggtggaaa aatttggact
    11701 ggatcggctg cgggctctca agaagtcgga aatcagcgcg cgggcgcggc actttcacaa
    11761 gatgactcat ttcagtttgt agagctccgg cgggattatc tcgacacatt cagaaaatcc
    11821 ccgcctctga agaggcgggc tacagtgctg tagcccccga cttcggaccc gagcttttct
    11881 gcggctgcaa gtaccgcttc cagggcgcac gcagacgttg tattcagggc aacatcccac
    11941 gctgacaaat agtttgctct cagcttccac gttgtatact ttttttgtag cgctcactca
    12001 tgttgaaaag tcgaaaacca ttcgcgctcg ctccggggag cacaattgcc gtggtcgcac
    12061 cagccagttc cacgaaagaa gagcgcattc agcgcggctg cgaaacattg gaaagtctcg
    12121 attttcgagt caagcgctac tcctcgaaac ataaaccaat ggattttttc tcggctccgc
    12181 tcgccgagcg ccgcaaacaa ttgcaagacg cgctgatgcg gcctgaaatt gcggcggtcc
    12241 tgtgttcgcg cggcggatat ggcagcacgg aaatattaga tggattgaat acggcgcgat
    12301 tgaagcaacc aaaaatattt tgcgcattca gcgatctgac ctcagtccac atttacttgt
    12361 ggcagaagct gcgatgggtc actttttacg gcccgctggt ggcgggtggc ttcgatgcgg
    12421 gtccgaatgc caccaacggt ttcgatccgg acacgtttat gtgggcgatg accgcgacga
    12481 acagcggctg gagcgtgccg ctggaaggcg aaacgttttc gcgcggtatt gccgagggaa
    12541 ctttgctggg cggctgtatt acgttgattg aaacttcgat cggtacgccg tgggaactcg
    12601 atacgcgcgg ctcgatttta ttgttagagg accggggagt gaaaccctat catctcgatc
    12661 gcatgttgat gcatttgaag caggccggta aatttgaggg agttcgtgga attattttgg
    12721 gagaattccc ggattccgaa acgcccgaag gtagctcgat gagtgtggct gaagtttgca
    12781 aacgactgct cggtccgcta catattccta tcgtgtacgg cgtagcagtg ggccatacgc
    12841 agcgccccat gttgacgatg ccgcttggcg tgcgcgcgaa actccatgcg acgggtgaag
    12901 gccgcatcga catcctggaa ccggcggtgc gcgcatgacg cgacctttac attttcatct
    12961 ctcgggaatt ggcggcgcgg ccatgacgcc tttggccgga atgctcgccg caagcgggaa
    13021 ccgcgtcaca ggttcagatg ccggcgtcta tccgcccgcg tccacgcttc tcgaaagttt
    13081 gggcattaac tggaaaaatg gattcgacgc tgcgaatctc gatcctgcgc cggatattgt
    13141 ggtgatcggt aatgcactct cgcgaggcaa tccggaagtc gaagccgtac tggataataa
    13201 aattccatac cgctcgcttc cacaaacact cgaggaatat tttctgccgg gtcacgattc
    13261 gctggtcgta accggaacac acggtaaaac gacgacgacg tccatgcttt cgtggatttt
    13321 gcaccatgcc ggacggcatc cgaattttct gatcggcggc gtggccgaaa atttcggacg
    13381 gagttttgga ctgggcggcg gaaaagaatt tgtgctggaa ggcgatgaat atgattccgc
    13441 atttttcgat aaggccccga aattccttca ctatcacccg caggaaatga ttctcacgtc
    13501 tcttgaattc gatcacgcgg atatttacga aaatcttgcg gccatcgagc tgcaattccg
    13561 ccgcctggtg aatctggttc ccaagagtgg cagaattgta gcctggggcg agagcgaaac
    13621 cgtgcgcgga gttttgcaaa aggcgtactg cacggtggag acgtatggtt ttgcgcccgg
    13681 agtggattgg cttgcgggcg atctggaaat cagggatggc gaaacgcatt ttcgagtctc
    13741 gcgcagcggg aaagaagtgg cgcgggtgaa aatggctcta gcgggccgtc acaacgtgtt
    13801 aaacgcgact gccgccgttg caatcgcgca cggccgcggc gtcagcggag aagcgattgc
    13861 cggcgcgatg gctgaatttc gcggcgtcct gcggcggctg gaaataaaag gcgagccttc
    13921 cggtgtgctt gtggtcgatg atttcgcgca tcatcccact gcgatccgcg caacgattga
    13981 cgcggcgcgc catcgctggc cgggcagaaa attgtgggcc gtgttcgagc ctcgctccaa
    14041 caccatgcgg cgcaaggtct ttgaaaaaga tcttgccgat tcgctggcca ccgcggatgc
    14101 ggccgtactg ggcgccgtaa atcgcgccaa tctcttgagc gatgccgagc gcatgtcgcc
    14161 ggcgcgcgtg ctggacacga ttaccggcgc gggacatagc gctgcaggat tcgattccgt
    14221 ggacgaaatt gccgaatatc tggcttcaga aacgcggcct ggcgaccttg tcctcgtgat
    14281 gtccaacggc agctttgatg ggctgtgcgg caagcttctc ggcaaattgc aggaacgtgg
    14341 cgctccggca gggaaatcca gggcatgatg ttccgtcttc gtccgtggtc ctgcgcggca
    14401 attcttgccg cgatttttta tttttcgtct cccgcgcacg ctaccattga atacacagtt
    14461 tcgctcaccc acccagagag acacgttttt aacgtggcca tgcgcattcc caatgtgcgc
    14521 gatcacctga cggttcaaat gcccgcgtgg aatgcgctct atcagattcg cgatttttcc
    14581 tcgcacatca tgcaggcttc ggccaaggac caagccggaa aaccgcttcc gatggcgaag
    14641 ctcgacaagc aaacctggag cgtgtcggcg agtggaaccg tcactgttac ctatggaatt
    14701 ttctgggatg aatcaggtcc atttgcatcg caactaaatt ccgaacatgc gttcattaat
    14761 tctgccatgc tcctgctgta tgtccctgat cgcagagcag aagataccaa aatagtattc
    14821 gatgatgtcc cggaaggctg gcgagtggca gtggagttgg aggcggcagc agtatccgcg
    14881 ggccaccacg cgggcgctta cgtggcgcca agctatgatg cgcttgtcga cgcgccagtg
    14941 gaaatcggcc attttgagga attccatacg gaggctggcg ggagaccaat acgcatcgtt
    15001 gttcacggag atgcgggcga ccattcaaag cttgtagaat ctctgaagaa aattgtcgat
    15061 tacgatgtga cgctcatggg tggagcgcca tttcgcgagt acatgtttct atttcacgtc
    15121 ggaaataatt tcggcggcgg cggtatggag cacatgaatt gcaccgcgat tgcagcggac
    15181 tttcccgcgc aattacccag ctactccgcg cacgaatttt ttcatgcctg gaatgtgaaa
    15241 cgcattcgtc cgcaagcatt ggaaccagtc gattacacga aggaaatgta tacgcgtgcg
    15301 ctgtggttcg ccgagggagt gaccaataca tacggatcgt atgcgctgga acgctcgggt
    15361 ctgtggtcgg cgcaacaatt ctacgccaat ctcgcggacc agatcaggga actgcagtcg
    15421 cgccccgcgc accgctggca gagcgtcgag caatcgagcc ttgattcctg gtttgagaaa
    15481 tatccgcttt acaaccgtcc cgaggaaagc atctcgtact acaacaaagg gcaaattctg
    15541 ggcgtcctgc ttgatatcgt cattcgcgat agaactgaca accacgcaag tatggacgat
    15601 gtgcttcgcg cactgaacga gcagtatgcg aaggcgggcc gcttctataa tgaaagcgat
    15661 ggactgcgcg ccataatgga agaagtgatc cgcaaaaagg atccgggagc gattcccgac
    15721 ctttctgatt ttttcactcg ttacgtggcg ggaacggacg aaatcccgta ttcggatttt
    15781 ctctcgtttg ctggattggc actgcatgag agccagcggc gcgccgcctt tggattcgcc
    15841 attagccacg acactgcttc taatgtgacg attgcctccg tcgatcggga cagcggtgcg
    15901 ggcgaggcgg gcttgaagga aggcgatgtg ctcttgcaac tgaatggcga aagttttcca
    15961 cgcatcccgg agcgctggtt gcgagatcat caacccgatg agcgcgtcac tgttaaaatt
    16021 aagcgtggtg gggaagtatt ggatatttct ttcccgcttg gccgcgtcac ggattcgacg
    16081 ttccagatta cggaaatcgc gtcacccacg gaaaggcagc ggcgcattcg caatggaatt
    16141 ctgcgcggca taaccactcc ctgaaatgat tcgctcagcc tttgtcgcca tagcgatgtc
    16201 gctctgcacg cttgtgctcg ggcctccgct gattttactg acgctcatta cgaaatccgc
    16261 cacgctgatg tattggactg gcgtaaagat ggtcgtttac gttacgcgaa ttgcgggaga
    16321 gaaagtcacg gtggaaggcg ttgaaaatat tccaccgggc gtgtgccttt ttgcggccaa
    16381 ccacacgagt aacgccgatg cgccagcgat cgtgggcgcc attcctcgac gcatcgcgat
    16441 tcttggaaga aaatcactct tcgatattcc ggttgtcggt atggcatttc gcctggcgaa
    16501 atttgttccc gtggatcgcg gcaaccgcga cgccgcgctc gaaagcgtta aaatagctgt
    16561 gcaatatatt cacgaggggt tttcattcct tgtctatccg gaggggacac gcagtcctga
    16621 cgggcggctc cagcggttca agaagggttc cttcgcgatc gccatcgaag ccgggattcc
    16681 catcgttccg gtcgcttgcg cgggggcaca ccgcatcatg aagaagcatt cgctgatcgt
    16741 ccggcccgga aaggtgacgg tacaattcgg aaaaccgatc gacgcgtcaa aatttacaat
    16801 cgagcagcgc cacgagttgg cgcaaactgt ttatgaagcg attgcggccg atttgccgga
    16861 agaccaaaag ccaatgcagt agatatttaa ccgcaagccg tatttctcac tgattcatcg
    16921 aataatttct cgcgatttca tttcatggct gaaggttcgg cgaaatggaa attgtggggg
    16981 cgcggcatgc cgtaccccta tagctcatct gatttcttgt gctcgatctc gccggttagc
    17041 ctgcgaactt ttttctgaag ttccggcagc cgcttgtagg ctgaaaacgc tttccggtac
    17101 gttctgttat ccatcagcgg cgagccagac cagagccctg cagcaggaat atcctggtgg
    17161 atg
//
LOCUS       DASZMB010000170         3538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4018325, whole genome shotgun sequence.
ACCESSION   DASZMB010000170 DASZMB010000000
VERSION     DASZMB010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4018325"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /locus_tag="VG272_07775"
     CDS             <1..1200
                     /locus_tag="VG272_07775"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HWF13618.1"
                     /translation="EQGYYLGFANEGIWPLCHIAHTRPVFRADDWEYYQRVNEKFAQA
                     VLEEIEGADRPFVLIQDYHFALLPSLIKAKRPDARVAIFWHIPWPNAEAFRICPWEHE
                     LLEGLLGADLVSFHIQAHCNNFLETVDQSLQSRIQWDRFTVNRNDHLTLVRPHPISVA
                     LPDGVDDIAPEQNRAEILARLGPEVLFFGVGVDRIDYTKGLLERFRGIESFLEKFPLY
                     REQFAFAQFGAPSRTAIKRYLDFFREVEAEAERINRRFQTENWKPIHLFSRHHSHREI
                     EPFYKAADFCLVTSLHDGMNLVAKEFVVSRSDQDGVLILSQFTGASRELHDALLVNPY
                     DSGQMAEAIRQALEMPSEERQLRMKRMRRVVREHNIYRWAAELILELSEIRLDAPELA
                     DTSRSST"
     gene            1278..2057
                     /gene="otsB"
                     /locus_tag="VG272_07780"
     CDS             1278..2057
                     /gene="otsB"
                     /locus_tag="VG272_07780"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HWF13619.1"
                     /translation="MLHTKKAVPHLFHCWNQVSDRIRAAEEIRLFLDFDGTLVHIYPT
                     PEQVKLPAAVRRILSKLSSHPRMRVALVSGRRNSVLREFIRVPRIQLLGLYGWERKGG
                     MVLPARTRKALPLLRAVIKSLPTKFPGIRIEQKGVSFAIHFRGAPHQAIRRAKIWTRR
                     FMTHIRADFAVVRASSAWEIVPRQVKGKGVALRELLKGLRTPFLPIYLGDDLTDEPAF
                     QVLRRGITIRVGPAAPTSAHFRLKDASEVRDFLERLQKTVP"
     gene            2103..>3538
                     /locus_tag="VG272_07785"
     CDS             2103..>3538
                     /locus_tag="VG272_07785"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HWF13620.1"
                     /translation="MLTNFCDILIFDAGFTGYLLYTMRFIRIIGILGLLIAYVVPAAA
                     QRERGELQIEVHDQQGAALAAAGELVSEGNQFHLNFQVGSEGRYTAQDLAFGVYRVTL
                     TLPGFSPATQLIEIRSIVPQHLAVALRLKSVKTQMEVSDQETLVDTSRANTVYTIGSQ
                     TISEQMPSQPGRGIQDAVDAQPGWLYEANGTLHPRGSEYDVQYVIDGTPVTENRSPAF
                     APPLNADDAESVRVMTAGFPAEYGRKLGGVVEVTSPKNNPTGLHGEIDVEGGSFSTGS
                     LHGALFYSSGGNRFSFSGDGFHTNRYLDPPVLGNYTNLGNSGGYSASYQHDFANGDRV
                     FATFTRHAVRYLVPNELIQQQNGQRQDAAQEETGGQIHYTHAFSTDVLLSVAGSVRDT
                     TALLSSNALSTPVIISQDRGYREAYARADLAGHHGRNDWKIGADGIFNPVHESLQYQL
                     TDPTQFDPGTALRFQFSDHRWDIEPSAYV"
BASE COUNT          768 a          979 c          960 g          831 t
ORIGIN      
        1 gagcagggct actatctggg attcgcaaac gaaggtattt ggccgctttg ccatatcgcg
       61 catacacgtc cggtatttcg cgccgacgat tgggaatact accagcgcgt caatgaaaag
      121 tttgcgcaag ccgttctcga agaaatcgag ggcgcagatc gcccgtttgt gctgattcag
      181 gactaccact ttgcattgct tcctagtttg atcaaggcca aacgccccga cgctcgcgtc
      241 gctatttttt ggcatatccc ctggcccaat gccgaagcgt tcaggatctg tccttgggag
      301 cacgaattat tggaggggtt gctaggcgcg gatctggtca gttttcatat tcaggcgcac
      361 tgcaataatt ttctggaaac cgtggatcag tcgctgcaga gccgtatcca atgggatcgc
      421 tttacggtca atcgaaacga tcatttgact ctcgtgcgtc cgcaccccat cagtgtcgca
      481 ttgccggatg gagtcgacga cattgcgccc gagcagaatc gtgcggaaat cctggcgcgg
      541 ttgggaccgg aggttctttt cttcggagta ggcgtcgatc gaatcgatta caccaagggc
      601 ctgttggagc ggtttcgcgg catcgaaagt ttcctggaaa aatttccgct ctatcgtgaa
      661 caattcgcat ttgctcagtt cggcgcgccg agccgcacgg ccatcaaacg ttacctcgat
      721 ttcttccgtg aagtggaagc agaggcggaa cggattaacc ggcgttttca gaccgaaaat
      781 tggaagccga ttcatttgtt cagcaggcat cacagtcatc gggaaatcga accgttctat
      841 aaggctgccg atttttgctt ggttacttct ctgcatgacg gcatgaattt ggtggccaag
      901 gaattcgtcg tctcacggtc ggaccaggac ggcgtgttga ttctcagcca attcaccggt
      961 gccagtcgcg aattgcatga tgcccttctc gttaacccat acgatagcgg ccagatggct
     1021 gaggctatcc ggcaagcgct cgagatgcct tctgaggaac ggcagctacg tatgaagcga
     1081 atgcgccgtg tcgtacgaga gcacaatatt tatcgctggg cggcggaact gatactcgag
     1141 ctatcggaga ttaggctgga tgcaccggaa ttggcggaca ccagtcgaag ttcaacttag
     1201 ttatcttttt atttttatca ttttatttaa tcgcgaggga aaacttccgt cgattccggg
     1261 tctccactcc atttctcatg ctccatacca agaaggccgt tccccatcta tttcactgct
     1321 ggaatcaagt ctccgatcgt attcgcgccg ccgaagaaat ccgcctgttt cttgatttcg
     1381 atggcacgct cgtgcacatt tatccgactc cggaacaggt aaaattgccg gcggcggtcc
     1441 gccggattct gtccaagctc agcagccatc cgcgcatgcg cgtggcgctc gtgagcggtc
     1501 gccgaaattc cgtgctccgc gagttcattc gtgtcccacg tattcagctt ctgggactct
     1561 atggatggga aagaaaaggc ggtatggttt tgccggcgcg cactcgaaag gccttgccgc
     1621 ttcttcgcgc ggtgatcaaa tctttgccaa ccaagtttcc cggcatccgc atcgaacaga
     1681 aaggggtaag ctttgcgatt cattttcgcg gggctccgca tcaggccatt cgccgcgcga
     1741 aaatttggac gcgcaggttt atgactcaca ttcgggccga tttcgcagtc gtccgggcaa
     1801 gcagcgcttg ggaaatcgtt ccacgtcagg tgaaaggcaa aggcgtagcc ctgcgtgagc
     1861 ttttgaaagg actgcgcaca ccgtttctgc ccatttattt aggagacgat ttaaccgacg
     1921 agcccgcatt tcaggtgctg cggcgtggaa tcacgattcg agttggcccc gcggctccca
     1981 cgagcgcgca tttcaggctc aaagacgcta gtgaagtccg ggattttctg gaacggctgc
     2041 aaaaaaccgt tccatgagca atttgcatcc ctggaacaga aatagttctg gatgtgcgat
     2101 gtgtgctaac gaatttctgc gatattctta ttttcgacgc tggatttacc gggtacctcc
     2161 tgtatacgat gcggttcata cggatcatcg ggattctcgg ccttctgatt gcttatgtcg
     2221 ttcccgcggc cgcgcagcgg gaacgcgggg aacttcaaat tgaggtacac gatcagcaag
     2281 gcgcggcact cgcagccgcg ggcgaactgg taagcgaggg caaccagttc catttgaatt
     2341 tccaggttgg aagcgaaggg cgttataccg cgcaggacct cgcatttggc gtctaccggg
     2401 tcaccctgac gctgccagga ttttcgccgg ccactcaact cattgagatc cggtccatcg
     2461 tgccgcagca tctcgccgta gccttgagac tcaaatcggt caaaacacag atggaagttt
     2521 ccgatcagga aaccctggta gacacctccc gagcgaacac ggtttataca attggctcac
     2581 agactatcag cgaacagatg ccttctcagc caggccgagg catccaggat gcagtggatg
     2641 ctcagccggg ttggttgtat gaggccaatg gcacgttgca tccacgcggt tccgaatacg
     2701 atgttcaata tgtaattgac ggcacgcccg tgacggagaa tcggtcgccg gcctttgcgc
     2761 cccccctcaa tgctgacgat gcggagtccg tgcgcgtaat gaccgcgggt ttccccgctg
     2821 agtacggcag aaagcttggc ggggtggtgg aggtgacttc cccaaaaaat aatcctaccg
     2881 gactgcacgg cgaaatcgac gtcgaaggtg gcagtttctc aaccggttcg ctccacgggg
     2941 cgctgtttta ttcatccgga gggaacagat tctcctttag cggcgatgga tttcatacca
     3001 accgttacct cgatccgccc gtgctcggca actacacaaa tttggggaat tcaggcggct
     3061 actctgcatc gtatcagcac gatttcgcaa acggggaccg cgtcttcgcc accttcacac
     3121 gacacgcggt gcgctatctg gtgccaaacg aactcatcca gcagcagaat ggccagcgcc
     3181 aagacgccgc ccaggaggaa acaggcggcc agatccacta cacgcacgct ttttcaaccg
     3241 atgtcctttt gagcgtcgca ggcagcgtgc gcgacaccac cgctcttctt tcctcaaatg
     3301 cgctgtccac tcccgtgatc atttcgcagg atcgtggtta ccgcgaagca tacgcgcgcg
     3361 cggatcttgc cgggcatcac ggcagaaacg attggaaaat cggcgccgat ggaattttca
     3421 acccagtcca cgaatcattg caatatcagc tcaccgatcc cacccaattt gatcccggca
     3481 ccgcgctgcg gtttcagttc tcggatcacc gctgggacat cgagccatcc gcgtatgt
//
LOCUS       DASZMB010000171         7161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4574476, whole genome shotgun sequence.
ACCESSION   DASZMB010000171 DASZMB010000000
VERSION     DASZMB010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7161
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4574476"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VG272_07790"
     CDS             <1..472
                     /locus_tag="VG272_07790"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HWF13621.1"
                     /translation="YTEFKLVIQENKMAHVTLNNPKLTLEGAEAILDAAKAQAARIGV
                     PMNIAVVDDGGFLMAFARMDGAKPASIEIAFNKAHAAAIRRQNTGPARSGNEVSVLLS
                     LGLAIGSHAHQTPIRGGLILEMNGQVVGAIGVSAGSEDEDTEVARAGVAAFVKG"
     gene            562..1881
                     /locus_tag="VG272_07795"
     CDS             562..1881
                     /locus_tag="VG272_07795"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HWF13622.1"
                     /translation="MPNLKQSALKIFRHTLAGIDIPASMQRKLCRAGSEIVVNGIAYD
                     LADYENIFAVAIGKASVAMAHGIADTLVPNFRPEGIVVAPTAVPDPPDGFTAIVGGHP
                     IPNEGSFTAGKTILDLLARASEKTLVFFLLSGGGSALVELPLDPAVTLGDFQALNRTL
                     VTCGASIDEINAVRKHLSAVKGGRMAVAAGSSTKITLGVTDVPEGQESALASGPTLPD
                     PTTVADACSVVRRYDLRSKLPVRIRRCFENPEFIVETPKAENPAFDAQRSTFQIMLGR
                     HELFHTAHHCSESLEFVTICDNTTDNWPVENALDFLLGQLVALKQSNPRKSVAVIADG
                     EVSSPVTGDGIGGRNLAFVLNAVSKIAGRTIAVLSAGTDGIDGSSPAAGAVADGETLA
                     RAHAMGLDPAEYFRRSDSYTFFQKLGDAIETGATGNNLRDLRILLME"
     gene            complement(1975..4614)
                     /locus_tag="VG272_07800"
     CDS             complement(1975..4614)
                     /locus_tag="VG272_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HWF13623.1"
                     /translation="MNFLRKRYLVRLPAAILTLAVLLAGGARADRPYAPSREYDLQNV
                     RVALHFDIDQRKVIGDVTHTLTTLRDGETHLDFDCSELAVSSVRVNGKDATFDLRDNN
                     LRVSLAQPAKSGEKFEVEIKYDGKPTSGLYFILPDQGNPNRAKEIWTQGEAEDTHHYI
                     PIYDYPNDRTAFEIILTVPADWLTVSNGKLISVQDAPNAQKTWTWRQSQPVSTYLISF
                     VAGEYQTKKDSWKNIPVTYNVPRGMGDTIDATFARTKQMLDFFSDRFGVVYPWDQYAQ
                     TAVHDFVASGMENVSATTLAARDMLHAGLAGERPEAADSLVSHELTHQWFGDLVTCKD
                     WTNTWLNEGFATFGAELWEEHFYGLDASSYRYWRDQNSWMQSRELFSIPIVTRNIDDS
                     VEYEGNVYDKAGWVIHMLREQMGDEAFFRALKHYLEANRMQNVVTADLVKSIEESSGT
                     NVDRFFDQWIYGAGAPRFTVRYSYDETAKTVNLSVKQTQKVEGRVGLFRSPIDISITT
                     ASGEKVFPIEVSKASESFSFAVDGAPQIVLFDKGDKILKSVDFQKTAEEWIRQLRTAS
                     SVPDRADAAVALGTFKDNDAAVSALGDVALHDKFWGVREEALRSLGRINSPAARKQVL
                     ADLSNEQPWVRVVAVEQLGKDRGDEEVIKRLQNIFKDDKAYTVRAAALQSLALDKSSN
                     LQATLEKTLTISSPGDVLRGAALRAMGSLGDDAVVPSLLEWSAPGKPADLRGIAIGAL
                     GRVDLKNRDITAKLISYLNEMSFDIRFATIFALGRRGDPTAIEPLEALLKTGELSIGV
                     PHALENLIEELKTKSAQQGGPGATAPAASASSSGNQKNPDAGAANINQAVLDRLDQVE
                     HQLADMNEKLKHIESSMPDNKND"
     gene            complement(4764..5120)
                     /locus_tag="VG272_07805"
     CDS             complement(4764..5120)
                     /locus_tag="VG272_07805"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWF13624.1"
                     /translation="MSKLEKLSWKTIETEQLTDNLSRQMVSGESATVSQLFLKKGATV
                     PRHSHVNEQYSWIISGSLNFIFDDKEVFVGAGELLIIPANVPHGAVALEDTVDVDIFT
                     PRREDWIRKDDGYLRK"
     gene            5337..5546
                     /locus_tag="VG272_07810"
     CDS             5337..5546
                     /locus_tag="VG272_07810"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaP-like transmembrane domain"
                     /protein_id="HWF13625.1"
                     /translation="MPAESNVSGMARVLYLAGGAALSMWGLWGADQGWTQWTWLAAGG
                     LLLVLGLIGYSPVHAIVSKSHKKAD"
     gene            5547..6128
                     /locus_tag="VG272_07815"
     CDS             5547..6128
                     /locus_tag="VG272_07815"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HWF13626.1"
                     /translation="MVFLHSVSGFFLFFELPIPLYWIILHPFNPFWRPRVRTAFWFAG
                     LFAWTTGGILLWHFRFYLMSYERPSWFSIFAGFALIFIEIYLFARVERELGTRRLVGH
                     AELTGTGEFFAGGLYAYVRHPRYTGMFCAVIGSALLSGTDWMWITIAWWFPFALIVIG
                     LEERELMARFGPSYEAYRKRVPAFIPLGRSTAK"
     gene            6244..6333
                     /locus_tag="VG272_07820"
     tRNA            6244..6333
                     /locus_tag="VG272_07820"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6278..6280,aa:Ser,seq:cga)
     gene            6457..>7161
                     /locus_tag="VG272_07825"
     CDS             6457..>7161
                     /locus_tag="VG272_07825"
                     /inference="COORDINATES: protein motif:HMM:NF024502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase SAM-like domain-containing
                     protein"
                     /protein_id="HWF13627.1"
                     /translation="MIGVYIRTKVDGKRTFRAAPDDPGDFEYWLKSTHDGRQKWHRVG
                     RYDAVKKAKLLLEREQERKEAARKYGLVAPSAIGHVTVSEGVEKFIQRKVAAGKRPGT
                     VTAYKFTLGLFQEFCPHLYVEQITGDTLLDFAAWCRKRGDAERTVANRFVDLASFMKF
                     HGKAGLVPKSDWPKYTERRVESYTQDELDKFYKAAKTDKERLLLDLLIHSGFRIGEIA
                     HLTFGDVDFTGNKLHVQ"
BASE COUNT         1571 a         2002 c         1902 g         1686 t
ORIGIN      
        1 ttacacagaa ttcaaattag ttattcagga gaacaaaatg gcgcatgtaa cgttgaacaa
       61 cccaaaactt actctggaag gcgcggaagc gattttggat gcagccaagg cgcaggccgc
      121 gcgcatcgga gtgcccatga atatagccgt cgtggacgac ggcggatttt taatggcttt
      181 tgctcgcatg gacggggcca aacccgcctc cattgaaatc gcatttaaca aagcgcatgc
      241 cgccgccatc cgccgccaga acacaggtcc cgcgcgtagc ggcaacgaag tcagcgtcct
      301 gctctcattg ggtctcgcga tcgggagcca cgcgcaccag acgccgattc gtggcggact
      361 tatcctcgaa atgaacggcc aggtcgtagg cgcaattggc gtaagcgccg gctcagaaga
      421 cgaagacacg gaagtcgcgc gcgccggcgt agcagcattc gtaaaaggat aatttcagta
      481 ggacaggctt cagcctgtct tcttttgatt tttttctggg ataacataag tcaaacagac
      541 aagcctgccc gactcagcac catgccgaac cttaagcaat ccgcgctgaa aatttttcgc
      601 cacacgctgg cgggcatcga tattccggct tccatgcagc gcaaactctg ccgcgcaggc
      661 tcagaaatcg tagtcaacgg catcgcttac gatttggcgg actacgagaa tatttttgcc
      721 gtcgccatcg gcaaagcttc cgtggccatg gctcatggta tcgctgatac actcgtcccg
      781 aattttcgcc cggaaggaat tgtggttgct ccgactgccg taccggatcc gccagacgga
      841 tttacggcta tcgtcggagg gcatcccatt ccgaacgaag gaagtttcac tgccggaaaa
      901 acgattctcg atctgctcgc ccgcgcgagt gaaaaaactc tagttttctt cctgctttcc
      961 ggaggaggct cagcgctcgt cgaacttccg ctggacccag cggtcacgtt gggcgacttt
     1021 caggcgctga atcgcacgct ggttacgtgc ggtgcgtcga tcgatgaaat caacgcggtt
     1081 cgaaaacact tgtctgcggt gaaaggcggc cgcatggccg tcgccgcagg atcgtccaca
     1141 aaaattactc tgggcgtgac ggatgttccc gagggccagg aatcagcgct ggcctccggc
     1201 cccacccttc ccgatcccac gaccgttgca gatgcatgtt ctgtggtgcg gcgttatgat
     1261 ttgcgttcaa aactgccggt tcgcattcgc aggtgtttcg aaaatcctga attcatcgtc
     1321 gaaactccga aggcggaaaa tcctgcattt gatgctcagc gctccacgtt tcaaattatg
     1381 ctgggacgcc atgaattgtt tcacaccgcg catcattgtt cagagtcgtt ggaattcgtc
     1441 acgatatgcg acaacaccac cgataattgg ccagtcgaaa atgcactcga ttttcttctg
     1501 ggccaacttg tcgctcttaa gcaatcaaat ccgcgtaaat ccgtggcggt aattgcggat
     1561 ggcgaagtga gttcaccggt gacgggtgac ggcattgggg gaaggaacct tgcgttcgtc
     1621 ctgaacgcag tcagcaaaat tgcgggacga acaatcgcgg ttttaagcgc gggcacagat
     1681 gggattgacg gctctagtcc cgccgcggga gcagttgcgg atggcgaaac tttggcgcgt
     1741 gcgcacgcca tgggcctcga tccagcagag tattttcgcc gcagtgattc ctacacattt
     1801 tttcaaaagc ttggcgacgc aatcgaaacg ggtgcaactg ggaataattt acgcgacctc
     1861 agaattttgc tgatggaata atcgggatgg tcttatgtgg cgcgggcttc agcctgcggg
     1921 gtttggtcct tgcgaagact gtctgccgca ggctgacgcc cacgccacat ccgctcagtc
     1981 gtttttgtta tctggcatgg acgattcaat atgcttcagc ttttcattca tgtccgccag
     2041 ctgatgctcg acctgatcga gcctgtctag cactgcctga ttgatgttcg cggcgccggc
     2101 atcaggattt ttctggttgc ccgaagagga agctgaagcc gccggagcgg tcgcgcccgg
     2161 ccctccctgc tgcgcactct tggttttcag ctcttcaatt aaattttcca gcgcgtgcgg
     2221 aacgccgatg gaaagttcgc ccgtcttcag caacgcttcc agcggctcga tggcggtggg
     2281 atctccgcgc cgccccagcg caaagattgt cgcgaaacga atatcgaaag acatttcatt
     2341 caaataagaa attagctttg cggtgatgtc acgatttttc aaatccactc gaccgagcgc
     2401 tccgattgcg atcccgcgaa gatccgccgg tttgcccggc gcagaccatt ccagtagcga
     2461 aggtaccact gcgtcgtcgc cgagcgaacc catcgcgcgg agcgctgctc cgcgcagcac
     2521 atcacccggt gaagatatcg tcagcgtttt ttcaagagtc gcttgcaggt tggaagactt
     2581 atccagcgcc aacgattgta aggcagccgc gcgcacggtg taggccttat cgtctttaaa
     2641 gatattttgc aggcgtttga tcacttcttc gtcgccacga tcttttccta actgttccac
     2701 cgcaaccacg cgcacccacg gctgctcatt ggaaagatcg gcgagcacct gcttccgcgc
     2761 cgcaggcgaa tttatgcggc ccagcgaacg cagagcttct tcgcgcacgc cccagaattt
     2821 atcatgtagc gccacgtctc cgagcgcgct tacggcggcg tcgttatctt taaacgtccc
     2881 cagcgcgacc gccgcgtccg cgcggtccgg aacgctcgat gccgtgcgca gctggcggat
     2941 ccattcctcg gccgtttttt gaaaatcaac agacttcaga atcttgtcgc ctttatcaaa
     3001 cagcacgatt tgtggagcgc catcaacagc aaatgaaaac gattcgctgg ccttggaaac
     3061 ttcgatcgga aatacttttt cgccgctcgc cgtggtgatc gaaatatcaa tgggagagcg
     3121 gaacagcccg acccgcccct ccactttttg cgtttgcttc acgctgagat ttactgtttt
     3181 cgcggtttca tcgtacgaat atcgcaccgt aaagcgtggg gcccccgctc cataaatcca
     3241 ctgatcgaaa aatcgatcca cattcgtccc ggatgactcc tcgatgctct ttaccaggtc
     3301 cgcggtgacc acgttttgca tgcggttcgc ttccagatag tgtttgagcg cgcggaagaa
     3361 tgcttcatct cccatctgct cgcgcagcat gtgaatcacc cagcccgcct tgtcgtaaac
     3421 atttccttcg tactccacgg agtcgtcgat attgcgcgtg acaatgggaa tcgaaaacag
     3481 ctctctggat tgcatccaac tattttgatc gcgccagtag cgataactgg aagcatccag
     3541 gccatagaaa tgttcttccc acaactccgc gccaaacgtg gcgaaccctt cgttcagcca
     3601 tgtatttgtc cagtccttgc acgtcacaag gtcgccgaac cactgatgcg tcagctcgtg
     3661 cgaaacgaga ctatcggctg cctccggccg ttcgccggca agaccggcat gcaacatgtc
     3721 gcgcgccgcg agtgtcgtcg cgctaacgtt ctccatgcca gatgccacga agtcatgtac
     3781 agcggtctgc gcatattgat cccacgggta aaccacgccg aatcgatcgg aaaagaaatc
     3841 cagcatttgc ttggtgcgcg caaacgttgc gtcaatcgtg tcgcccatac cgcgaggaac
     3901 gttgtaggtc accggaatat ttttccagct atctttcttg gtctgatatt cgcccgccac
     3961 aaaagaaatc aggtaggtgg aaactggctg ggattgccgc cacgtccatg ttttctgcgc
     4021 gtttggcgca tcctgaacgc ttattagttt tccgttggac accgtgagcc aatccgcagg
     4081 caccgtgagg attatttcaa acgcggtgcg gtcatttggg taatcgtaaa tgggaatgta
     4141 atgatgtgta tcctccgcct caccctgggt ccagatttcc ttggcgcggt tgggattgcc
     4201 ttgatcggga agaataaaat agaggcctga ggtaggcttg ccgtcatatt taatttcgac
     4261 ttcaaatttt tcgcccgatt tggcgggctg tgcgagtgaa acgcgcaaat tgttatcgcg
     4321 caggtcgaac gtcgcatcct tgccgttgac gcgcacgctg gaaacggcaa gctcggagca
     4381 atcgaaatcc agatgcgtct cgccatcgcg cagcgtcgtc aatgtgtgcg tcacatcgcc
     4441 gatcaccttg cgctggtcga tatcgaagtg cagcgcgacg cgcacattct ggagatcgta
     4501 ctcgcggctt ggcgcgtaag gccggtccgc gcgcgcgccg cccgcaagca ggacagccag
     4561 agtcaaaata gccgccggga gccggactaa ataacgtttc ctgagaaagt tcattccggc
     4621 ctcgcaaggg aatttgcggc atcaaaatac atcaaatgat tatacctgcg cgtcccttgc
     4681 ggaatcagag caagcttttt tcccattgct attagacgcc agactggccg attggtttcc
     4741 tgtggccacg cacgccgccg agcttacttg cgcagatatc catcatcctt gcgaatccaa
     4801 tcttcgcgcc ggggagtaaa aatatccacg tctacagtgt cttccagcgc caccgccccg
     4861 tgtggcacgt ttgccggaat gatcagcagt tcgccggcgc cgacgaatac ttccttatca
     4921 tcaaatataa aattgagtga tccggagata atccacgaat actgctcgtt cacatgggag
     4981 tgccgcggca cggtggcgcc ctttttcaaa aagagctgtg aaacggtggc gctttcgccc
     5041 gacaccatct ggcgcgacag attatctgtg agctgctcgg tttcgatggt cttccaggat
     5101 aacttttcaa gcttactcat gatcggctcc ttagattgaa atgttcagga cagcatcgtc
     5161 tactggcagc acgcctcgcg tcaactgaca gtgcagcccc tctatcttcg ctaggcgcta
     5221 ggaatgatca gccgctgaca ccaatccccg atatctcgat tcgtaacaat caaccatgga
     5281 cgggacatct gctaagatgc gtgtcgctcg tagatccaat caagtgaggg cattcgatgc
     5341 cggcggaatc aaatgtgtca ggcatggcgc gggtactgta tctcgccggc ggcgccgctc
     5401 tttctatgtg gggcttatgg ggcgctgacc agggctggac gcagtggacc tggctggccg
     5461 cgggaggatt gctgctggtt ctcggcttga tcggctattc tccggtgcat gcgatcgtct
     5521 caaaaagtca caagaaagcc gactgaatgg tttttctcca cagtgtctcc ggattttttt
     5581 tattctttga gcttcccatt cccctgtatt ggataatcct tcacccgttc aatcccttct
     5641 ggcgtcccag agttcgcact gctttctggt tcgccggact attcgcgtgg actaccggcg
     5701 gcattctgct ctggcatttc cgattttatt taatgtccta cgaacgcccg tcgtggtttt
     5761 caattttcgc tggattcgcc cttattttta ttgaaattta tcttttcgcg cgggttgagc
     5821 gtgaactcgg cacgcgaaga cttgtcggcc acgccgaact gaccggcaca ggagaatttt
     5881 ttgccggcgg tttgtacgcc tacgtgcgcc acccgcgcta caccggtatg ttctgcgccg
     5941 ttatcggctc cgcgctgctc agcggaacgg actggatgtg gatcaccatt gcatggtggt
     6001 ttccgtttgc cctcatcgta attggcctcg aagagcgcga gctaatggct cgctttggcc
     6061 ccagctatga agcttatcgc aagcgcgtcc ccgcttttat tccgttagga agaagcaccg
     6121 cgaagtaaaa ataattttca aagaatttcc tgatcgtgac tcagcggctc ccgaatcgga
     6181 cttcctacac aatcactgcc gttgcccggt tcctccagct actatagaat gacttgcctc
     6241 tgcggagagg tgtccgagcg gttgaaggag ctcgcctcga aagcgagtgt cggggaaacc
     6301 cgaccgtggg ttcgaatccc accctctccg ccataatttt ctaaaccatc taaaacgaat
     6361 gtgatacgtg gcgtaacctg gtcgggttac gcgcgggtta cggaatgaac gctgcgactc
     6421 ccagaaaaag ccgtattagc caaaagggag acggcgatga ttggcgttta cattcgtact
     6481 aaagttgacg gaaagcggac cttcagagca gcaccggatg atcccgggga tttcgaatac
     6541 tggctgaaat ctacgcacga cgggcgtcag aagtggcatc gtgtcgggcg atacgacgct
     6601 gtaaaaaaag ctaagctttt gcttgagagg gagcaggaaa ggaaagaggc cgcgcgcaag
     6661 tatggattgg tggccccgtc ggcgatcggg cacgtgacgg tctctgaggg agtggaaaag
     6721 ttcattcaga ggaaggtcgc ggctggaaaa cgacccggca ccgtaaccgc ctacaaattc
     6781 acgttgggat tgtttcaaga gttttgcccg catctttacg tcgaacaaat taccggcgac
     6841 acgctcctag acttcgcagc ctggtgtcgc aagcgcggcg acgccgaacg gaccgtcgcc
     6901 aaccgcttcg ttgacctcgc ctcattcatg aaattccacg gcaaggccgg cctcgttccg
     6961 aaatcagatt ggcccaaata taccgagcgc cgagttgaat cgtacacaca ggacgaattg
     7021 gataaattct acaaggccgc taaaaccgat aaagagcgat tgctcctcga tttgctgatt
     7081 cattccgggt tccgcatcgg tgaaatcgcg cacctcactt tcggagacgt tgatttcacc
     7141 ggcaataaac tccatgttca g
//
LOCUS       DASZMB010000172         6690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4576234, whole genome shotgun sequence.
ACCESSION   DASZMB010000172 DASZMB010000000
VERSION     DASZMB010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4576234"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VG272_07830"
     CDS             <1..370
                     /locus_tag="VG272_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWF13628.1"
                     /translation="LTLRTAKTGTDVRIPLHPDAIAGLDRIQTDNYYFWSGLSTKKSC
                     VGDYQRAFKKLYELAKIKNGHAHRWRDTFAVELLLKRVPVADVAVLLGHQSVKITEKS
                     YSPWVIARQEQLEDTVRKTF"
     gene            complement(436..794)
                     /gene="ssrA"
                     /locus_tag="VG272_07835"
     tmRNA           complement(436..794)
                     /gene="ssrA"
                     /locus_tag="VG272_07835"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1091..2635)
                     /locus_tag="VG272_07840"
     CDS             complement(1091..2635)
                     /locus_tag="VG272_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13629.1"
                     /translation="MKTFAAPGVTVLNSGNTTSPASAFSAAADFLRLNPFRALAISAA
                     VLIPCFWHRRIEAGDLASHTYNAWLAQLTERGQAPGLWLAKQWNNVLFDMSLIRLGAL
                     AGLGLAEKICVSAAVLIFFWGAFAMISAISHRTPWSLTPCIAMFAYGWTFQQGFLNYY
                     ISLGLAFFGIALLQRGRGLERALILALLALAWMAHPLGAALLLGIGFYVVAAAKTKAR
                     NQPLLFTGAAFLLIGVSNYIVSHYPVFWPDRATLQAFLTHSGADQLLLYSARYRLPSD
                     LLVAFVPICIAVDAYFRRKREEPCSIYALPLQLYGLAFLAGILLPSAVQLSAYAAPLG
                     FLQARLTSVTAVFGCCLLGTVRPQKWHLPCLAAITVIFFLLLYGDTGKLNKMEEQIEN
                     YARAIPPGQRVIANIGPVPGSRLLISHIVDRACIGQCFSYGNYEPSSGQFRVRAHPGN
                     PFVISDSSGFNQIEGGSYVVRAEDLPISGIYRCKMNEADLCMRELKVGERNGLEDVQR
                     VATPIR"
     gene            2884..3897
                     /locus_tag="VG272_07845"
     CDS             2884..3897
                     /locus_tag="VG272_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13630.1"
                     /translation="MAAEPNAVEQAVSRTLRDNLEKIQRSVDELHQAVNDLSSACASS
                     RAGNALPPMVRAQTCAASLSATLEVLSRLVTSALQPPVQMPQLETETPRVSPSINFPP
                     PPVNLPVSVFEQPPLASTHPIEHITWAPPAPVAPVAPMPMPSVADIFQPVEPVQAILE
                     TPPAEATIFSGMSPAAEISAPVQPAPETQPELAKVIVPVTLPDPEPDFDLGVPVAVHA
                     GLEAPTSVERAEAGPVFDLDLLAPDEQELHRRAFRVAKVSMQDIKMLRPEDVRAGREN
                     KDLCFRLRDDIEKAHKEYGRRFQSIQSHPVDYFYDWMVEILGGGDPQTLGEYPYPSAV
                     LRR"
     gene            3836..6310
                     /locus_tag="VG272_07850"
     CDS             3836..6310
                     /locus_tag="VG272_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HWF13631.1"
                     /translation="MAEIHKRSASILTPLRSCGDNRKAPARKFGYVGILAALAALAMF
                     TIATSELRAAAPQNTAKKTGKKSSSKKAPVKTPVAAATANASATPLEKQLTQLSRALR
                     DQPNATAYSALSAFATRNAKNEIGARAALALGYYDINRDKPELASGWLHKASGEKMLR
                     DYVLYWQAEASVAMGQKDVALEQFQSILRDYPGSAITEPTVMALAQTAIAITKPADAL
                     AALNAYPNTTARPALMLLRAQARQALSTMNGEKATAAGTDYLDIYYRFPLNDEAKTAG
                     QQIPVLQSVLGDALPAVPLQTQIARAEAFYLAKRWRDASTEYANLMPKLAGVDHQRAD
                     LRIVQCEVELGGKLDQLVEIPLTDPELDAERIFSIAQAHRGLKLEAQLLDDVEELQNK
                     YPQSSWTAEALFSVGNFYWVNLDRVRATDFYLRSLAISPDGKNAFTADWRVAWTAYLD
                     RKPEAADMLEAYIRRFPSSSYVQDALYWIGRSYEHSGNLELARNFYLAGAKRFPLTYF
                     GAKAADRIRREPEGIGATPMTLTDFSLPIPAAPALTSLDQPVVVAAQERQARARVLSE
                     IAFDSSAELEYRAAYAATHSPKFLIDAAGAAIAAGHYGAGMAAVRQAIPQLEARRITD
                     VPNEAWRAAFPLPYEINLRSAAARNLLDPMLVAGLIRQESGFESKALSRAGAIGLMQV
                     EPTTALKLARQLKVRYARARLTDPGYNLQLGSRYLANLIQAFGTREAALAAYNAGEDR
                     VEQWSAGQNYLETAEFVESIPFTETREYVQIVIRNSDAYRSIYGPISPGDREPTRLST
                     KENRKTVAAQPVAATDTQPAAAGETR"
     gene            6307..>6690
                     /locus_tag="VG272_07855"
     CDS             6307..>6690
                     /locus_tag="VG272_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWF13632.1"
                     /translation="MSTGEASVIRTGMAAVVQKTLSAKAERFTESVIREMTRLAFQHD
                     AVNMAQGFPDFPASEEVKEAARQAITDDINQYAITWGAKELRDAIARKFERTQGISVD
                     PEREITVTCGSTEAMMATMMGIINPG"
BASE COUNT         1541 a         1866 c         1862 g         1421 t
ORIGIN      
        1 gctgacactg cgaacggcga agactggaac cgatgttcga ataccattgc accctgacgc
       61 tatcgctggt ctcgaccgaa tccagaccga caactattat ttctggtctg ggctgtccac
      121 gaagaagtca tgcgttggcg actaccaaag agcgtttaag aagctttatg aactggctaa
      181 gatcaagaat gggcatgccc accgatggcg ggataccttt gccgtggagc tactactgaa
      241 acgcgttcca gtagccgatg tggcggttct gcttggccat caatcggtca agatcaccga
      301 aaaatcatac tcaccgtggg tcattgcgcg acaggaacag cttgaggaca cagtacggaa
      361 gactttttga accccacaat ttcgctacaa ttatttttaa ttgtctgtaa ctgatacaaa
      421 atacattagt ttgtttggtg gaggcggggg gagttgaacc cccgtccgaa aagcgttacg
      481 gctcgaagcc tacatgctta gctcaattcc attggtttcg cccgccacgc tcagaatgag
      541 caagaaacgc agctgactag tccgattggt tctcgccgtc tcgccgcgga ccgagactcg
      601 acggccagcc tactgtgtga cgtttctgac tgggcccgta ggcgaagccc agggaaacgt
      661 aaccgtccta gtttaagcta ggcggccaat gccagttgtt gattggcagt tctagttttc
      721 cacccgattg agggcaggtg gcgccctgca tgccttcgag tcgcgacagc ttccgtcgaa
      781 accgtgtcgc ccccttttat tattttacca ctttttgacg ttgggcagtt gagagttgac
      841 agttgtgagt ttagagttcg cggaccggag gcagggaagc aaggaggcta ggaggcaagg
      901 aaaatccgaa accacaaatc aaatcagcag cgtgccaggc tgggtttcct aaatcgtctt
      961 tagttcatag ttaaattttt cacgcacctt tgattcgcgc tcacagtatc aatctccgac
     1021 accttttctc cgccaccttt gccttctgct tctttgcctc atggcacttg cccgtgggtt
     1081 tcgcagaggc ctatcgaatt ggcgtggcga cacgctgcac atcctctaag ccgtttcttt
     1141 cacccacttt aagttcgcgc atgcacaagt ctgcttcatt cattttgcac cggtagattc
     1201 cggaaatcgg caagtcctcc gcacgaacta cgtagctgcc gccctcgatt tgattgaaac
     1261 cggaactgtc tgaaatcaca aatggatttc cggggtgcgc gcgcacgcgg aattgtcccg
     1321 aggacggctc gtaatttccg tagctgaaac attgcccgat gcaggcccgg tccactatgt
     1381 ggctgatcaa gagtctcgat ccgggaaccg ggccgatatt ggcgatgacc cgttgtcccg
     1441 gcgggatggc gcgcgcgtag ttttcaattt gctcttccat tttgtttaat tttcccgtgt
     1501 caccgtacag gagtagaaag aagataactg tgatcgcggc gaggcaaggc aaatgccact
     1561 tttgcggtct gaccgtaccc aataggcagc acccgaagac cgcagtcacg gaagtcagcc
     1621 gggcctgcaa aaatcccaaa ggagccgcat aagccgaaag ctgcacggcg cttggaagca
     1681 gaatgccggc caggaaggcg aggccgtaaa gctgaagcgg caacgcataa atgctgcacg
     1741 gctcttcgcg cttccggcga aaatatgcat ccacggcaat acagattgga acgaaggcaa
     1801 ccaacaggtc cgaaggcaac cggtagcgtg cgctatagag aaggagctgg tctgcgccgc
     1861 tgtgcgttaa aaaggcttgc agggttgctc tgtccggcca aaaaacagga tagtggctga
     1921 caatatagtt gctcactcct atcaacaaga aggcggcgcc agtaaacagc agcggttgat
     1981 tgcgcgcttt agtctttgcg gcggccacca catagaaacc gatcccaagc aagagcgccg
     2041 cgccaagcgg gtgagccatc catgcgagcg ccaacaacgc cagaatgagt gcgcgttcga
     2101 gtcctcttcc gcgttgcagc agcgcaatgc caaagaacgc gagtcccaga gaaatgtagt
     2161 agttcagaaa tccctgttga aatgtccacc cgtacgcgaa catggcgatg caaggcgtga
     2221 gcgaccacgg agtgcgatga gaaatcgcac taatcattgc gaaggcaccc cagaagaaaa
     2281 tcaagacggc tgcggaaaca caaatttttt ccgccagtcc cagacctgca agggctccca
     2341 accggattag gctcatgtcg aataagacgt tgttccactg cttcgcaagc cacagtccag
     2401 gggcttgacc gcgctcggtc aactgcgcaa gccaggcgtt gtaggtatgg ctcgcgaggt
     2461 ctccggcttc aatcctcctg tgccagaaac agggaatcag cactgccgcg gaaatggcca
     2521 aggcccgaaa cgggttcagc cgcaggaagt cagcggcagc cgaaaacgcg gacgcgggag
     2581 atgtcgtatt tccggaattg agtacagtaa cgccaggcgc cgcgaacgtt ttcaacttct
     2641 cttgtagcgg gctcggctgt tgcgcgaaat gcgacttcat gtttcgatgg tagcagccgc
     2701 ttggggattg tcagattggg acctgcggcg tcgttccaaa agaaccaaca cataatcttt
     2761 tgcggacgaa gttgaaatcg gtcccttgct cgtgccgaaa ccgttgcagg atgttgagtt
     2821 gacaatcctt ccacgtggtt ccgataatca ggtttggggc caacccacgg gaggggcaag
     2881 ggtatggcgg cagaaccaaa tgcggttgag caagcagtat cgcggacact tcgggataat
     2941 cttgaaaaaa ttcaacgctc tgtggacgaa ttgcatcagg cagtgaatga cttgagttct
     3001 gcctgcgcca gcagtcgcgc gggcaatgcg ctgcctccga tggtgcgcgc gcaaacgtgc
     3061 gctgcttcgc tctccgccac gctggaagtt ctttcgcggc tggtgaccag cgcgctgcaa
     3121 ccgcccgtac aaatgcccca gcttgaaacg gagactccgc gagtttctcc ttcaatcaat
     3181 tttcctccgc cgccagtcaa ccttccggtc agcgtttttg agcagcctcc actcgcgtca
     3241 acgcatccta tagagcatat aacctgggcc ccacctgctc cggttgctcc tgttgcgccc
     3301 atgccgatgc caagcgtggc tgatattttt cagccggttg agcccgttca ggcgatttta
     3361 gaaacgccgc ccgcagaggc aaccatattt tctggaatga gtccagcagc cgagatttcg
     3421 gctcccgttc agcccgcacc agagactcaa cctgaattgg cgaaagtgat cgttcccgtc
     3481 actctcccag acccggaacc ggatttcgat ctgggtgttc ctgtggccgt gcatgcgggg
     3541 ttggaagcgc ccacatccgt tgagcgtgcg gaagcgggcc cggtttttga tctcgacttg
     3601 ctggcaccgg acgagcaaga attgcaccgg cgcgcattcc gcgtggcaaa agtttcaatg
     3661 caggacatca agatgttgcg ccctgaagat gttcgggccg ggcgggaaaa taaggatctt
     3721 tgttttagac tgcgtgatga cattgaaaag gcacataaag aatacggtcg ccgcttccaa
     3781 tcgattcaat cccatcctgt cgattatttc tatgactgga tggtggaaat cctgggtggc
     3841 ggagatccac aaacgctcgg cgagtatcct tacccctctg cggtcctgcg gcgataatcg
     3901 aaaagcgccc gcccggaaat ttggttacgt cggcatcctt gctgcgcttg cggccctggc
     3961 gatgtttacc attgccacgt ctgaactgcg cgcggccgcg ccgcaaaaca ccgcaaaaaa
     4021 aacaggcaag aaatcttcgt ctaaaaaagc gccggtaaaa actccggtcg ctgcggctac
     4081 cgcgaatgcg agcgccactc cgcttgaaaa acaactcacg caactttcgc gagcgcttcg
     4141 cgatcagccg aatgcgacag cgtattccgc gctctccgca ttcgccaccc gaaacgcaaa
     4201 aaatgaaatt ggcgctcgcg cggcgctcgc gctgggctat tacgatatta atcgtgacaa
     4261 gccggaactg gcttcaggtt ggctgcacaa agcgtccggc gaaaaaatgc tccgcgatta
     4321 tgttttgtac tggcaggcgg aagcatcggt cgcgatggga cagaaagacg tagcgctcga
     4381 acagttccaa agtattttgc gcgattatcc gggaagcgcg attaccgagc ctaccgtaat
     4441 ggctctcgca caaacggcca ttgcaatcac aaaaccggcg gatgccctgg cggcgctcaa
     4501 tgcctatccg aataccacgg cgaggccagc cttaatgctg ctgcgcgcgc aagctcgcca
     4561 ggcgctttcc acaatgaacg gtgaaaaagc tacggcggca ggcacggatt atctggatat
     4621 ctactaccgt tttccgttga atgacgaagc gaaaactgcc ggccagcaaa ttccagttct
     4681 gcagtcggtg cttggcgatg ctcttccggc tgtgccgctg caaacgcaga ttgcgcgcgc
     4741 ggaagcgttc tatctggcga aacgctggcg cgacgcgagc acggaatacg caaacttgat
     4801 gccgaagctt gctggcgttg accatcagcg cgccgatctt cgcattgtgc aatgcgaagt
     4861 ggaactgggc gggaagctcg atcagctcgt ggaaattccg ctgaccgatc cggaactcga
     4921 tgccgagcgc attttctcca tcgcgcaggc tcaccgcgga ctgaaacttg aggcgcagtt
     4981 gctcgacgat gtcgaagagt tgcagaacaa atatccccaa agttcgtgga ctgcggaggc
     5041 gctgttcagc gtgggcaatt tttattgggt aaatctggat cgtgtgcgcg ccactgattt
     5101 ctatcttcga tccctggcaa tttctccgga cggaaaaaat gcgttcaccg ccgattggcg
     5161 tgtagcctgg accgcgtatc tagatcgcaa acccgaagcc gccgatatgc tcgaagccta
     5221 cattcgccgt ttccccagtt ccagctacgt gcaggacgcg ctgtactgga ttgggcgttc
     5281 ttatgagcat tcaggaaatc tcgagctcgc gcgaaatttc tatctggccg gcgcgaaacg
     5341 ttttccgctt acctattttg gagcgaaagc tgctgaccgc attcgccgtg agccagaggg
     5401 aattggcgca acgccaatga ctctcaccga tttttcgttg ccgattcccg cagctccggc
     5461 gcttacatcg cttgaccagc ccgtggtggt cgcagcgcag gaacgacagg cgcgtgcgcg
     5521 cgttctcagc gaaattgcat tcgattcctc ggcggagctg gaatatcgcg ccgcctatgc
     5581 cgcgactcat tcaccaaaat tcctgatcga tgcagcaggc gcggccattg cagcggggca
     5641 ttacggcgcc gggatggccg cagtgcgcca ggcaattcct caacttgagg cgcggcgaat
     5701 tacggatgtt cccaacgaag cgtggcgcgc cgcttttcct ctcccgtacg aaattaattt
     5761 gcgcagcgcc gctgctcgca atctactgga tccgatgctg gtcgcagggc tcattcgcca
     5821 ggaatcggga ttcgagtcca aggcattgtc gcgcgcgggt gcaatcggcc tgatgcaagt
     5881 ggagccaacg acggcgctga aattggcgcg ccaattaaaa gtgagatatg cgcgggcgcg
     5941 attgaccgat ccgggttaca atttgcaact tggttcgcgc tatcttgcca acctgatcca
     6001 ggcttttgga acgcgggaag ccgcgctggc tgcctacaat gccggcgagg atcgcgtaga
     6061 gcagtggtca gcgggccaga attatctcga gacggcggag tttgtcgaat ccattccgtt
     6121 caccgagaca agggagtacg tgcaaatcgt gattcgcaat tcggatgctt accgttccat
     6181 ttacggcccg atttcaccgg gagatcgcga accgacgcgc ctctcaacca aagagaatag
     6241 gaagactgtg gcagctcaac ctgtcgccgc cacggacaca cagcctgctg cggcgggtga
     6301 aacccgatga gcacgggcga agcttcggtc attcgcacgg gcatggcagc tgtagttcaa
     6361 aaaacacttt ccgcgaaggc agagcgcttc acggagtccg tcattcggga aatgacacgc
     6421 ctcgccttcc agcacgatgc cgtgaacatg gcacagggat ttccggattt tcccgcttcc
     6481 gaggaagtga aggaagcagc ccgccaggcc atcactgacg acatcaatca atatgcgatc
     6541 acgtggggcg caaaagaact gcgcgatgcg attgcgcgaa aatttgagcg cacgcaaggc
     6601 ataagcgtcg atcccgaacg cgaaatcacc gtaacctgcg ggtcgaccga agccatgatg
     6661 gccacgatga tgggcatcat caatccgggc
//
LOCUS       DASZMB010000173         9393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3480523, whole genome shotgun sequence.
ACCESSION   DASZMB010000173 DASZMB010000000
VERSION     DASZMB010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 9393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9393
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3480523"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VG272_07860"
     CDS             <1..434
                     /locus_tag="VG272_07860"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWF13633.1"
                     /translation="IVEYRNHSSPRIRFTVACALGNFPNHPGAIETLLTLTQDADDDV
                     RDWATFGLGVLSDSDSPEIRDALFGRVSDSNEDVREEAMVGLGKRIDPRVLPPLITAL
                     EQPRMTDRVREAANLMLKIDEDRIDWTGHDYVAALRQQFSL"
     gene            710..1540
                     /locus_tag="VG272_07865"
     CDS             710..1540
                     /locus_tag="VG272_07865"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWF13634.1"
                     /translation="MTTSPAALTLAGMGSRSRYPGSFGPRVSWGGMLTPAIRNIVIAC
                     TGVLLLQTILKLSIFPVSYTIYFNHLFGLVPYYITRRFYLWQLVTYLFLHDGIWHLIF
                     NMLFLYFFGVDLEKTWGTRRFYEYYFITGIGAGIITVLINIIRDPRGGYSSGIPTIGA
                     SGAIFGVLIAAGIMFPDRQVWLIPFPIMIPMKLLVAGSIAIEFFLSLEVSGDTVSHIT
                     HLGGALVGYLYLRRGLFLYRFRNDYLDWKRRRTRRKFEVYMREHKDEPPRDSDDRWVN
                     "
     gene            complement(1753..3198)
                     /gene="pyk"
                     /locus_tag="VG272_07870"
     CDS             complement(1753..3198)
                     /gene="pyk"
                     /locus_tag="VG272_07870"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HWF13635.1"
                     /translation="MNFRAAKIVCTLGPASNSSEMIERLMRAGMDVARLNFSHGTHDD
                     HARTIKRIRESSARLSQPIGILADLQGPKIRTGLLKDKQPVQLVSGQRFTISIKEFAG
                     SAEGVSTTYKRMARDVSRGDRILLGDGLIELHVLSTTSTEVQCKVINGGLLGEHKGIN
                     LPGVKLKIPAVTAKDRQDLIFALQQGANFVAVSFVRDAKDVMLAKKAILQAKHDVPVI
                     AKLEKPEAIEHLDEILQVSDAVMVARGDLGVEMSPEKVPVVQKQIIARACEARRPVIT
                     ATQMLESMTQNPRPTRAEASDVANAVFDGSDALMLSAETASGAYPLEAVQMMDRIIRE
                     AEANNSHVLRPSPAQFNIAETASELICHASEELHMRVIAVFTETGSTARLISKHRPRR
                     PIIAFSTIQETRRRMSLNWGVVPRTIGKVQDIEELVQVAEKKLLEEKLVRPGDVVGIV
                     AGTPLFVGGTTNFMKFLVVGSDEGQLRRVIR"
     gene            complement(3252..4679)
                     /locus_tag="VG272_07875"
     CDS             complement(3252..4679)
                     /locus_tag="VG272_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWF13636.1"
                     /translation="MFHRAGRFFLTVALTALAAVSAHAQSPAASPDLNKLADEAQGWL
                     SDLVRINSVNPPGNEAAVAKYISAIFVKEGIPNEVLEMAPGRSIVVARLQAGPMPDPA
                     NALLLVAHQDTVGVDTSKWTVDPFSATIRDGYMWGRGSVDDKAMLAANIATMVQLKRS
                     KARLSRDVLFLATDDEEQGGSASIKIAVQKYWDKIACAYALNEGGRVIVKNGKVQYVG
                     VQASEKVAYNVIVTATGSSGHGSLPKPDNAVVHLAAAVQKLGTFQVPSQPSTITLRYF
                     EQLAKIEDDEIGKWMRALEQPERADLAVKHLAEQSPMWNSMLRDTIAPTMLQAGVRVN
                     VVPSEATANLNIRMLPGHSVEELIGQFTKIVNDPQIKFQLGPDPGENAPPSDLKTTLF
                     QTIERLTPQEFPGAITVPFLGTGATDSASLRLHKVQAYGLEPFPMMEEDSSRVHADNE
                     RMPIDGFHKGVVFLYHVVADFASTK"
     gene            5506..6600
                     /locus_tag="VG272_07880"
     CDS             5506..6600
                     /locus_tag="VG272_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWF13637.1"
                     /translation="MRLLTVPRVFRRLVLGAVMLTLAVPPGELSAWAQDQNKDKQNKD
                     KQDKNQQEPPAQTGPGFSISVTVPVVSLDVVVTDNNGNYLKDLKKENFRVTEDGTVQT
                     ITSFAPSEAPITIVLLLEFSKLGYEFFSYNARNWSAAFLGNLKPNDWVALESFNMRTN
                     VEVDFTHNRDEIMQGLYSMVFPPFSESNLFDALCDVLDRTKDVKGKKAVLVLASGIDT
                     FSKLTLDETLKRIRETDATIFTVGVGEQLFIAQAPGRFGQQLTYIQAQNQLKTFAAMT
                     GGRAWLPRFDGEIPSIMQEVAASLRNQYSMAYSPTNSTLDGKYRKIKVELVAPDGGPL
                     TVLNEKGKKVKFLVYARQGYTAPKSNIEPK"
     gene            6645..7808
                     /locus_tag="VG272_07885"
     CDS             6645..7808
                     /locus_tag="VG272_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWF13638.1"
                     /translation="MTAPARLETTRPILGSKLLWIFSFLIILIAVGIYFLRFELRSAA
                     FLIQFLDPQAKGLLVRLERHEFDTQEVTFPTPQGAVRARLYLPRGAAHSPGMVLVHGI
                     HHLGIDEPRLVSFARAAASSGFTVLTPEVVALADYHVDGASISTIGESAAWLEQRANT
                     GPVTVVGVSFAGGLSLLAACDPLYAPHIRSLVLVGAYDNLARVARFLATSQAEFPDGR
                     MEPYKAHDYGAAVFVYSHLDQFFPASDLVLAHDALRDWLWEEPAEAQALFSRLSPPSR
                     ATMEILLARKIDQIRPQLLNAIQEDDAQLSSVSPEGKLAALHAPVFILHGSTDDIIPS
                     TESLWLEKDIPHAYLRDALITPAFSHVNPDKNALWTDQARLVEFLAGVLRAAN"
     gene            complement(7879..>9393)
                     /locus_tag="VG272_07890"
     CDS             complement(7879..>9393)
                     /locus_tag="VG272_07890"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HWF13639.1"
                     /translation="PGNGSQGSAPAGEQQSRITYVTIAGSEPAGSFGGVAYKRTWGTV
                     AGVVAPRDTIQGFDQLPHDADGNYPYQSEFEITAPEKSGTNTVIFVEAENRGTPVFLN
                     ALHEVSVTGPPSATTNGGDTGNGFLFAHGTSYARVQWQAGVASNVPMQAEGVGEVIIR
                     DFGRMLAGRTPLNPKPTVDFGTYHTLLLGGVSLSGFFANTFIAEGFNADPVDGSAVFH
                     GAIAVDGTGNWLALNELAAQIGVKEFPYVVPDAKPLNASDILGGHLSDPFYVDIANYT
                     DFYRLRASVTDILPKNPRMRRYDWPSAHAPAPIDQSGGSARASRCNGGVVIDLNPIPH
                     SAYLRAVTLELEHELKVPSAKQAPSLPPSTLFALDPDPAPSKIPNVNHLPGLRIYLPL
                     VDEDAQPIGGVRFPDVEHPVGRAVPPSLPPVTTASIEATCGNLGGWQQFSPEELTKRY
                     GSEANYLKLYGQSVDRQIAAGFLLASDREELLKIASALYNRRPPAASQASGKSH"
BASE COUNT         2054 a         2551 c         2541 g         2247 t
ORIGIN      
        1 tcatcgttga gtaccgcaac cattcaagtc ctagaatcag attcacagta gcgtgcgcat
       61 tagggaattt cccgaatcac ccgggggcaa tcgaaacgct tctcacgctt acgcaggacg
      121 ctgacgacga tgtacgcgat tgggcgactt tcgggttagg cgttttaagc gattcagatt
      181 ctcccgagat tcgtgatgct ctttttgggc gagtcagtga ttcgaacgaa gacgtgcgag
      241 aggaggcaat ggtgggtcta ggcaagagga tcgacccgag agtcttgccg cccctaatca
      301 cggcgctgga acaaccgaga atgacggata gagtgagaga agccgccaac ttgatgctga
      361 aaatagacga agatcgaatc gattggaccg ggcacgatta tgtcgcggcc ttacgccagc
      421 agttctctct ctgagaaact cgatgcttcc caagtcatga taagtcatct tctcggaaac
      481 tggcttttct atggaatttg caatttctcc gccacgcttc cgcacgacta tccgtttcgc
      541 acggacacgc acgcaacaac gcaatcgctg ctcgccggga caattagaat ttagaaattg
      601 agagcgcgaa ttttgcttcg cacgcacgaa acacggttct gaatactctc aaacccattt
      661 cgaggggtgt atcacttttc cccgctctgt aaccttaccc tccgaatctt tgactaccag
      721 tcctgccgcg cttacacttg cggggatggg ttcgcgctca cggtatccgg gctctttcgg
      781 tcctcgcgtc agttggggcg ggatgctcac tcccgccatt cgcaatattg ttatcgcctg
      841 cacgggcgtg ttactccttc agacgatcct gaaactatcc atcttccccg tgtcgtacac
      901 gatttatttc aaccacctat tcggtttagt cccttattac atcactcgcc ggttttatct
      961 ctggcaatta gtgacttacc tgttccttca cgatggcatc tggcacctga tcttcaacat
     1021 gttgttcctt tattttttcg gagtggacct ggagaagact tggggaactc gccgattcta
     1081 cgaatattat tttataactg gaattggcgc gggcatcatt accgtcctca tcaatatcat
     1141 ccgcgatccg cgcggcggat actcttccgg cattccgacg attggagctt cgggcgcgat
     1201 tttcggagtt ctgattgccg cgggaatcat gtttccggac cgccaggtct ggctgattcc
     1261 atttccgatc atgattccga tgaaacttct ggtggcgggt tcgatagcga ttgaattttt
     1321 tctgtcactg gaagttagcg gagacacggt cagccacatc acgcatttgg gcggggcgct
     1381 ggtaggttat ttgtatttaa ggcgcggatt gtttctctac cgcttccgca atgattatct
     1441 ggattggaag cgccgtcgca cgcggcgcaa atttgaagtc tacatgcgcg agcacaaaga
     1501 tgagccgccg cgcgattccg atgatcgttg ggtgaactag gttttagtgg cgcgggcttc
     1561 tttagcctgc ggattttagg ccctgcaagg accaacctcg ctgaagagtc tgtgtgggaa
     1621 ctgcaatctt ggaacctcag cggctaaagc cgaacttatt ttgcagcgct tatgtcgtgg
     1681 ctaaagccac gacccacaat gagtttgaag ttctcacacg gactctgaag cccgcgccac
     1741 ataaagcaac tcctaccgaa tcacccggcg tagttgccct tcatcgcttc caaccactaa
     1801 aaatttcatg aagttcgtcg tgccgcccac aaaaagcggg gttcccgcca caattccgac
     1861 cacatctccg ggccggacga gtttctcttc gagcaatttt ttttcggcga cctgaacaag
     1921 ttcctcaatg tcttgcacct tgccgatggt gcgcggaaca acgccccagt tgagggacat
     1981 gcggcgccgc gtttcctgaa tcgtggagaa agcgataatc gggcggcgcg ggcgatgctt
     2041 ggaaatcaat cgcgccgtcg aacctgtttc cgtaaatacg gcaatcaccc tcatgtgtaa
     2101 ttcttccgag gcgtggcaaa tcagttcgga tgcggtttcc gcgatgttga attgcgccgg
     2161 gcttggccgc agaacgtgcg agttgttggc ttcggcttcg cgaatgattc ggtccatcat
     2221 ttgcacggct tctagcggat atgcgccaga ggcggtctca gcggacagca tcagcgcgtc
     2281 gctgccgtcg aatacggcgt tcgcaacgtc agaggcttcg gcgcgcgtgg ggcgcggatt
     2341 ctgcgtcatg gattcgagca tttgcgtcgc agtgatcacg gggcgccgcg cttcgcatgc
     2401 acgagcgatg atctgctttt gcacaacggg aactttttcc gggctcatct ccacgcccag
     2461 atcgccgcgc gccaccatca ctgcgtcgga tacttgcagg atttcatcca gatgttcaat
     2521 agcctcgggc ttttccagct tggcaatcac aggaacatca tgcttggcct gtaaaattgc
     2581 tttcttggcc agcatcacat ctttcgcatc gcggacgaaa ctgactgcga cgaaatttgc
     2641 accctgctgc aaagcgaaaa taagatcctg acgatctttc gcggtgaccg cgggaatttt
     2701 cagttttact ccgggcaaat tgatgccctt atgctcgcct aatagtccgc cattgatcac
     2761 cttgcattga acttcggtgg aagtagtcga gagcacgtgc aattcaatca gtccatcgcc
     2821 cagcagtatg cggtcgccgc gcgaaacgtc gcgagccatg cgcttgtacg ttgtgctgac
     2881 gccttcggcg cttcccgcaa attccttaat cgaaatcgtg aaccgctgcc ccgagaccaa
     2941 ttgcaccggc tgcttgtcct tcaacaggcc tgtgcgaatt tttggacctt gaagatccgc
     3001 cagaattcca atgggctgcg agagcctcgc ggaagattcg cggatgcgct tgattgtccg
     3061 cgcatggtcg tcatgcgttc cgtgtgaaaa attgagccgc gccacatcca ttccggcgcg
     3121 catcaatcgc tcaatcattt cggaggaatt actggcgggt cctagagtgc atacaatttt
     3181 agcggcgcga aaattcatgt ttgtttttta tccgatcccg atcagcgtgg aaggggggta
     3241 agtttcgttc gctatttcgt cgacgcgaag tcggcgacaa catgatatag gaagacgacg
     3301 cctttgtgaa atccgtcgat gggcatgcgt tcattgtccg cgtgcactcg cgagctatct
     3361 tcttccatca tgggaaaagg ttcgagtccg taggcttgca ctttatgcaa tcgcaagctg
     3421 gctgaatcgg ttgctcccgt tccgagaaaa ggaacggtga tcgcgcccgg aaattcctgc
     3481 ggcgtgaggc gctcgatggt ctgaaataat gtcgttttca agtcagaggg aggcgcattt
     3541 tcacctggat cgggcccaag ctgaaattta atttggggat cattgacgat cttcgtgaac
     3601 tgtccgatca gttcttccac ggaatggccg ggaagcattc tgatattcaa attggccgtt
     3661 gcttccgagg gaaccacgtt gacgcgcact ccggcctgca acatggttgg cgcaatcgta
     3721 tcgcgcagca tcgaattcca catcgggctt tgctcggcaa gatgttttac agccaggtcg
     3781 gcacgctcgg gctgctccaa tgcgcgcatc cattttccaa tttcatcgtc ttcaattttt
     3841 gcaagctgct cgaaatagcg cagcgtgatg gtgctgggct gtgacggcac ttgaaacgtc
     3901 cccaattttt gcacggcggc cgccaaatga accacggcat tatccggctt gggcagggaa
     3961 ccgtgtcctg aagatcccgt cgcggtaacg atcacgttgt acgccacttt ctcactggcc
     4021 tgaaccccaa cgtactgcac cttgccgttc ttgacaatca cgcggccgcc ttcattcaat
     4081 gcgtaggcgc aggcaatctt gtcccagtat ttttgtacgg caatcttgat actggctgat
     4141 ccgccctgtt cctcatcatc ggtggcaaga aagagaacat cgcgcgagag gcgcgctttg
     4201 ctgcgtttca attgaaccat ggtcgcgatg ttggcggcga gcatggcttt gtcatcaacc
     4261 gagccgcggc cccacatata accatcgcga atggttgctg aaaatggatc gacagtccat
     4321 tttgaggtgt cgacgccgac cgtatcctga tgggcgacga gcagcagcgc attcgcagga
     4381 tcgggcatgg gacctgcctg taaacgcgca acaacaatgc ttcggcccgg cgccatttcg
     4441 aggacttcgt tcggaatgcc ttctttcaca aatatggcgg aaatatattt tgcaacggcg
     4501 gcttcgttac ccggaggatt cacggaatta atgcgtacca ggtcgctgag ccaaccttgc
     4561 gcttcatcgg ccagcttgtt caagtcaggc gatgcggcgg gagattgcgc gtgcgcgctc
     4621 acggcagcaa gcgcggtcaa cgcgactgtc agaaaaaatc ggcctgcacg atgaaacatt
     4681 tcgcactcct ccgaaataaa attcttccac gctcgcaact tattcatcgt accatgcgtc
     4741 tctcgattgc aagacagcgc atgcgcaagc gtgtgccgcg aagaaaacca ggaccagagt
     4801 aaatgttgat ggaaattgcg gggctgtcct aatttgaaga gaattcgtgt ggcacggttc
     4861 ctttggaggt cgggacttcc gtcccgacga cgagcttggt ttggtcgggg tattaacccc
     4921 cctgaaattt caggggctga cgcccgtgac taaaggcaac ccaaatggcc aagttgaaac
     4981 tcggccctcc taaattcgag aaattttcaa attaggacac taccgaaatt gccggtagtg
     5041 gatcagtttg aatcttgact ctgcttggcg gtcttcgtct ttagcgtagc cttactatcg
     5101 agtggccgaa ctagaaccca tgctgtgttg ccgttgcaag aaccgcagcg gggaaattgc
     5161 tgaccatcga ggattgttcg ttctttcggg tggcacgccg cttcgtaaat tccagcaact
     5221 cgatttacgt cgcccgttct atctgacata gcaactgtag cctagcatga aagcgggacg
     5281 ctcgtacgtg attctgatca cacaggtgat ccgaggctta gcgcctgggt gtggttccaa
     5341 ccccatgaaa tttcggactg acccagtacc aaattgccga atcatttgaa catcccggcg
     5401 cttcgtggca gcatcgaatg tgcaagataa acactttcat ccaggccggc ggccaggtgc
     5461 agtttttatg gattcaggcg ccaaattacc gaggtccagc tagatatgcg attgctcacc
     5521 gtccctcgtg tgttccgaag attagtgctg ggcgcagtca tgcttacact tgccgtgcct
     5581 cctggcgaac tctccgcatg ggcgcaggat cagaataagg acaaacaaaa caaggacaag
     5641 caggataaaa atcagcagga gcccccggca cagaccgggc cgggcttctc aatttccgta
     5701 acggttccag tggtgagcct ggacgtggtg gtaacggaca acaacggaaa ttatttgaag
     5761 gacctcaaga aagaaaattt ccgcgtcaca gaagatggca cggttcaaac gattaccagt
     5821 tttgctcctt ccgaagcgcc aattacgatt gttctgctgc tggagttcag caaattgggc
     5881 tacgaatttt tttcctacaa tgcgcgcaac tggtcagctg cttttttggg taatctcaaa
     5941 ccgaatgact gggtagcgct agaatcgttc aatatgcgca cgaacgtgga agtggacttc
     6001 acccataacc gcgacgaaat catgcagggg ctttactcga tggtttttcc tcccttcagc
     6061 gaatcgaatc tcttcgacgc gctttgcgat gtgctggacc gcacgaagga cgtgaagggc
     6121 aagaaggcag ttcttgtcct tgcatccggg atcgacacgt tcagcaaatt gactctggat
     6181 gaaactctca agcgcattcg ggaaacggac gccacgatat ttacggtcgg tgtcggtgag
     6241 cagctcttta tcgcacaagc tcctggacgg ttcggccaac aattgactta catccaggcg
     6301 caaaatcaat tgaagacgtt tgcggcgatg accggcggcc gcgcctggct cccccgattt
     6361 gacggcgaaa ttcccagcat catgcaggaa gtggcggcga gcctgcgaaa tcaatacagc
     6421 atggcatatt ccccgacgaa ttcaacgctc gacggcaagt accgcaaaat aaaagtagaa
     6481 ttggtcgcgc cagacggcgg cccgctgacc gttctcaacg aaaaaggcaa gaaggttaaa
     6541 ttcctggttt atgcgcggca gggctatacg gcgccgaaat ccaacattga accgaaatag
     6601 ccgccgcacg atgcaggacg gtattcttcg gcagcctcgc caatatgaca gcaccggcgc
     6661 gattagaaac aactcgccca attctgggtt caaaacttct ctggattttc tctttcctaa
     6721 ttattttgat tgctgttgga atctattttc tgcgctttga actgcgcagc gccgctttcc
     6781 tgattcaatt tctcgatccc caggcgaagg gacttctggt gcggcttgag cgccacgaat
     6841 tcgatacgca ggaggtaacg tttcccactc cgcaaggggc cgttcgcgca aggttgtatc
     6901 tgccgcgagg agcggcgcat tcccccggga tggtgctggt gcacggtatt catcacttgg
     6961 gaatcgatga gcctcgacta gtgagctttg cccgcgcggc ggcaagcagc ggattcacgg
     7021 tgttgacgcc ggaagtcgtg gcgctcgccg attaccatgt cgatggcgca tcgatcagta
     7081 cgatcggcga gtcagccgca tggctggagc agcgcgccaa caccgggcct gtgaccgtcg
     7141 taggcgtaag ctttgcgggc ggactttcct tgctagccgc gtgcgatccg ctctacgcgc
     7201 cgcatattcg ttccctggtt ttggtgggcg catatgacaa tctggcgcgc gtcgcgcgat
     7261 tcctggccac aagtcaggcg gaatttccgg acggccgcat ggaaccttat aaagcgcatg
     7321 actacggcgc cgccgttttt gtgtactctc atctggatca gttctttccg gcaagcgatt
     7381 tggtgttggc gcacgacgcg cttcgtgact ggctttggga ggagcccgcc gaagcccaag
     7441 cgcttttctc gcggcttagt ccgccgagcc gggcgacaat ggaaattctc ctcgcgcgga
     7501 aaattgacca gatacggccg caattgctga atgcgattca ggaagacgat gcgcaactct
     7561 cttccgtttc gccggaggga aagttggctg cgctgcatgc gcctgtcttt attttgcacg
     7621 ggtccacgga cgatattatt ccttcgaccg aaagcctgtg gctggaaaaa gacatcccgc
     7681 atgcctactt gcgggacgcg ctgattacac ctgcgttttc gcacgtgaat cccgataaaa
     7741 atgccctgtg gaccgatcag gctcgcctgg tggagtttct ggcaggcgtt ctgcgcgctg
     7801 caaattaatt ttgtgctcag tgaaaaaaat tgaaaacgaa cggacatttt acaaaatgag
     7861 cgtcaaaaaa atcgcaaatc agtgcgactt tccgcttgct tggctcgctg cgggagggcg
     7921 acggttatac agcgccgatg cgatcttcag caattcttcg cgatcggaag ccaacaaaaa
     7981 gcccgcggca atttgcctgt ccacggactg cccatacagt tttaaataat ttgcttcgct
     8041 gccatagcgt ttcgtgagtt cctcagggga aaattgttgc caacctccca gattaccgca
     8101 cgtcgcttcg atcgaagctg tcgtaacggg cggtagagat ggcgggacag cgcggcccac
     8161 cggatgttcc acatccggaa agcgaacgcc gccgatcggt tgggcatcct cgtcgaccag
     8221 cgggagatag atgcgcagac ctggtagatg atttacgttt ggtattttgg atggcgctgg
     8281 atcaggatcc agcgcaaaga gagtcgatgg aggcagcgac ggcgcctgtt tcgccgatgg
     8341 aactttaagt tcatgctcca gttcgagtgt gactgcacgc aagtacgcgg agtgtggaat
     8401 aggattgagg tcgatcacta cgccgccatt gcagcggctg gcgcgggcgc tgccgccgct
     8461 ttggtcgatg ggcgcaggag cgtgcgcgct gggccagtca tagcggcgca tgcgcggatt
     8521 tttcggcaag atgtccgtga cgctggcgcg aaggcgatag aaatccgtgt aattcgcgat
     8581 gtcgacgtaa aagggatcgc tcagatgacc gcccaaaata tcagaagcgt tcagaggctt
     8641 cgcgtccggc acgacatacg gaaattcttt tactccgatc tgcgccgcaa gttcgtttag
     8701 cgcaagccag ttgcccgtac cgtcgaccgc aatcgctccg tgaaacacgg cgctgccgtc
     8761 aaccgggtcc gcgttaaatc cttccgctat aaatgtgttc gcgaaaaatc cgctgaggct
     8821 gacgccgcca aggagcaacg tgtgatatgt cccgaaatcc acggtaggct tgggattcag
     8881 cggagtccgc ccagcaagca tgcggccaaa atcgcgaata atcacttcgc ccacgccctc
     8941 tgcctgcatc gggacgttgc tcgccacgcc tgcttgccat tgcacgcgtg catacgatgt
     9001 gccatgcgca aaaagaaaac cgttccccgt atctccgcca ttcgttgtag cggacggcgg
     9061 ccctgtcacc gagacctcgt gcagtgcgtt gagaaaaacc ggcgtgccgc gattttccgc
     9121 ttcgacaaag atcaccgtat tagttccaga cttctccgga gcggtaattt caaattcgga
     9181 ttgataaggg tagttgccgt ccgcgtcgtg aggcagttgg tcaaatcctt gtatggtgtc
     9241 gcgtggcgcg acaacccctg caacagttcc ccaggtcctt ttgtatgcga ctcccccgaa
     9301 cgatcccgct ggctccgatc cggcaatcgt gacgtaagta atcctcgatt gttgttcacc
     9361 cgcgggcgcg cttccctggc tgccatttcc agg
//
LOCUS       DASZMB010000174         9870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2930664, whole genome shotgun sequence.
ACCESSION   DASZMB010000174 DASZMB010000000
VERSION     DASZMB010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 9870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9870
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2930664"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VG272_07895"
     CDS             <1..541
                     /locus_tag="VG272_07895"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HWF13640.1"
                     /translation="LRSGAKPGDLLCVSGRLGEAELGLRLILQKAQNQKLWPNLVKKH
                     FHPQPRLALGDWLASHRYATSMIDTSDGISTDLNHICKASGVGARVWAEKIPVVDLPP
                     GLRSLRLDPLDLALHGGEDYELLFTVPKKFSGRLPREIEKIPVTVIGEITREKKMILV
                     GADGRTKPLRPLGWDPFRK"
     gene            complement(757..3108)
                     /locus_tag="VG272_07900"
     CDS             complement(757..3108)
                     /locus_tag="VG272_07900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HWF13641.1"
                     /translation="MVDLPPITLNLRGRKVVGRVAFILLLLGSIALGTMAGLWFVYSS
                     DLPQIRALEDFQPDVVTELYADDGQVVGNFALQRRVLLTYEQYPQILKDAILTTEDQH
                     FDEHWGVDFTRVAGAAYKDLVSHRVVEGASTITMQLAGTLFLDRSDRHMGRKIQEALL
                     ALQIERHYTKQQIFTMYGNQIYLSHGNYGFEAASESYFGKPVGKLNIQESALLAGMIR
                     GPSYSPILHAQRALQRRNLVIELMARNGKITEAQAKQAIAQPLGLHQQSPRNTLAPYF
                     VEEIRKYLESTYGTEMVHQRGLRVYTTLNVNMQKSANRAIHDGLHAYDRRHGWRGKLD
                     NILADKSDTLESYEDDDWRWPISKGDYVQGLVTAADQKEATIKIGSLRALLTPPDFAW
                     TGRKLPSDLLKAGDLAEFYIKDISGNVAHVELEQNVAPQAAILAIDNPTGEIKAMVGG
                     YSFDESKFNRAMQAQRQVGSSFKVYVYAAAMEQGFTPFDTILDAPFTTMSGGQAYSPH
                     NYDEKFEGVITLRRALDGSRNVPAVKLAEKIGMNSVIDMARRFGITSPLPPYLPITLG
                     AADLNLLEHTSAFTVFPNDGIRVDPHMIRRVTTYDGALLEQAHPVVHDVVEPDVARTM
                     TAMLQDVVERGTGMAARALGRPAAGKTGTTNDFTDAWFMGFTPEITAGVWVGFDDKSV
                     SLGKPETGAVAALPIWLEFMEGALEGKPILAFQNVEPLDKVALTKTVKVDTPDTAPED
                     AGEGPTRISVNPTVPDVNPQVEVPAKATAPLQNSATPASTPRN"
     gene            complement(3151..3657)
                     /locus_tag="VG272_07905"
     CDS             complement(3151..3657)
                     /locus_tag="VG272_07905"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HWF13642.1"
                     /translation="MATTAISSEVSRRPAPWPATLLVIGRVALGAIFLIAAYAKLHFA
                     GEWHLRDYQFFFAMAIDSYKMLPVSAVQLMAQILPWFELVLGIFLIAGIGLRVIGTIT
                     SGLLLVFIGAMTRAKILGLEINCGCFGNNEKLGAATLIRDSSLLVLALAVTVGAFLIK
                     KRRERVSS"
     gene            complement(3670..4350)
                     /locus_tag="VG272_07910"
     CDS             complement(3670..4350)
                     /locus_tag="VG272_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWF13643.1"
                     /translation="MNFLTVRPIRVVTLVALCAMATTVFARPQTKSPVSPASKPTPAS
                     TAAPTFQKAIGNPSAPITMEVFGDFECPACRGFFEGTVKQVIDDYVIPGKVYIIHRDF
                     PLDIHPYARQAARLANAAAEFGQFEAMERTLYDHQDEWVAKGNIDQVIAPYFPPAQLK
                     KIEAFEAQHMNEINASIAKDQAMGTQRNVNQTPTIYITSHGKMEALPGGGVDYKLLKS
                     YFDYLLRQ"
     gene            4764..6710
                     /gene="dnaK"
                     /locus_tag="VG272_07915"
     CDS             4764..6710
                     /gene="dnaK"
                     /locus_tag="VG272_07915"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HWF13644.1"
                     /translation="MSKIIGIDLGTTNSVVSVMQGGEPVVIPNQEGGRTTPSVVAITK
                     TGERLVGQVAKRQAVTNPENTVYSIKRFMGHRFDEVTEETKRVPYKVVKGPHDDARVE
                     IFGKAYTPPELSAMILQKLKTAAEDFLGEKVSKAVITVPAYFNDSQRQATKQAGEVAG
                     LEVLRIINEPTAAALAYGLDKKKDETIAVYDLGGGTFDISILEVGEGVVEVKATAGDT
                     HLGGDDVDNRVVDWLIAEFKKDQGIDVSKDRMAVQRLKEAAEKAKIELSQMQETDINL
                     PFLTADASGPKHLQLKLTRAKLEQLMEDILQRTIGPTKQALSDAGITPDKIDEVVLVG
                     GSTRIPRVLQIVKDLFGGKEPHKGVNPDEVVAVGAAIQGGVLGGEVKDILLLDVTPLS
                     LGVETLGGVMTVLISRNTTIPTRKSEVFSTAADNQPSVEIHVLQGERKMAPDNRTLGK
                     FHLMGIPPAPRGMPQVEVTFDIDANGILNVSAKDRATNKEQKITITASTGLSKEEAEK
                     MRSDAEAHAEDDKKKLAEIEARNILDNRVYQVEKLIADNRDKLSETDTKPVEEAVAAA
                     KTALAGGILEQIQSATVTLESASHKLAEVLYKTAAAAGAQPGAPGSQDGGPGAAPHSG
                     PGPTPEGEVIDAEYVDADETKKPN"
     gene            6788..7171
                     /locus_tag="VG272_07920"
     CDS             6788..7171
                     /locus_tag="VG272_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13645.1"
                     /translation="MTTYEGIKRIGPPQAIQKLREDLFMVQIALTETPTADWKRLFYE
                     TQQAPPPDFLPRAVEIISNYIRFRSEVPSVQARTAWIDRWIDRANQKEASMGGRLDEE
                     RRKRRDEHQREQQELAAINAQWEKL"
     gene            7399..8556
                     /gene="dnaJ"
                     /locus_tag="VG272_07925"
     CDS             7399..8556
                     /gene="dnaJ"
                     /locus_tag="VG272_07925"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HWF13646.1"
                     /translation="MATTTKQDYYDLLGVSRKAAQKEIRAAYRKLARKYHPDLNPGDK
                     SSEEKFKQVQEAYDVLSDAKKRQMYDQFGFDTPGSGGTPAPGNGGDVHFDFGGFDFGG
                     QGAGGAGGGPSFKDLFSQFFRGGGATTEAPTRAEPGSSLEYQIEISFWEAVRGTVKKL
                     TISRLDVCTECRGTGTVGQPQTCTACGGSGQVTQTSGKMRFNLTCSRCGGSGRVRNIC
                     RVCGGEGRVRRADTIDIRIPAGVQTGSRVRVPGRGNAGSGGEPPGDLYIITDVQPHPF
                     FDRRGDDIYISVPITVTEAVLGAKIEVPTIDGPSRPLRIPPGTNSGQKLRLREKGVPS
                     VRTPGHRGDQYIELQVVVPKPVDERVKELLRELEKIAPENPRKDLFDRAKV"
     gene            8568..8972
                     /locus_tag="VG272_07930"
     CDS             8568..8972
                     /locus_tag="VG272_07930"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWF13647.1"
                     /translation="MARVTTKRKKTRAGYMISAVAELYKLHPQTLRLYERVGLLKPSR
                     SDGNTRLYTDSDLERLEVILTLTREMGVNLAGIEIILNMREKMAEMQQQMEAFTSFVQ
                     QELARGMRAAGSRQQGNALARVPVPKVIRVHK"
     gene            9440..>9870
                     /locus_tag="VG272_07935"
     CDS             9440..>9870
                     /locus_tag="VG272_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13648.1"
                     /translation="MAQPDCPICSGSGWKTVERVSEEENLKRVSWEKPYEGTNITRRV
                     WAVPCDCTGKDRATRVLNRARIPDRYQNCDFESFEKDVYDIGIHGPDAPAWNRSLEQA
                     KVVTEGFARNYPTGAETGLLLMGTYGTGKTHLAVAALRQII"
BASE COUNT         2396 a         2690 c         2782 g         2002 t
ORIGIN      
        1 attgcgctcg ggcgccaaac caggcgattt gctttgtgtc agcgggcgac tgggggaggc
       61 ggaacttggc ctgcgcctga tcctgcaaaa agctcagaat caaaaactat ggcctaacct
      121 ggtaaaaaaa cattttcatc ctcaacccag gctcgcattg ggtgattggc tcgcatcgca
      181 ccgatacgca acttcgatga tcgatacctc ggacggcatt tccaccgact tgaatcacat
      241 ttgtaaggcg agcggagtcg gggcgcgtgt gtgggcggaa aaaattccgg ttgtcgactt
      301 gccgcctggg ttgcgttctt taaggctcga tccactcgac ctcgcacttc atggtggaga
      361 ggattatgaa ttgctcttca cagtgccgaa aaagttttct ggccgtctgc cccgtgaaat
      421 agaaaaaatt cccgtaactg tgatcggcga gatcacacgt gagaaaaaga tgattctggt
      481 cggggcggac ggaagaacta agccacttcg ccctttggga tgggacccct tccgcaagta
      541 aagcctgaac aatttgttcg cggtaggatt gtccaccaca taaacgtgta atggcatggt
      601 ttacgctttt cgtagcggcc actccgttcc atggttctcg gaacggagtg gcgattttcc
      661 acttacccgc gcacgattta accatacgtc tgaattgcac tggaagaaaa gcgcccgccc
      721 gaaaaacggc cggccgctac gaaggcgacg ccatctctaa ttgcgcgggg tcgatgcggg
      781 tgttgcggaa ttttgcaggg gcgcagtcgc tttagcagga acttcgacct gtggatttac
      841 gtccggcacg gttgggttta cgctgatcct ggtcggacct tcgcctgcgt cttcgggggc
      901 ggtatccggc gtatcaacct tcacggtttt cgttagtgca accttatcca aaggctccac
      961 attttgaaac gccagaatgg gtttgccttc gagcgcgcct tccatgaact caagccagat
     1021 cggcaatgcc gcgacagcgc cagtttcggg cttgcccagc gataccgact tatcatcgaa
     1081 gcctacccac acacccgcgg tgatttccgg tgtgaagccc atgaaccagg catccgtaaa
     1141 gtcattggtc gtgccggttt tgccggcggc ggggcggccc aaagcgcgcg cggccatgcc
     1201 agtcccgcgt tcgaccacgt cctgaagcat ggcggtcatg gtgcgcgcca cgtccggctc
     1261 gaccacgtca tgcaccacgg gatgcgcttg ttcgagcagc gcgccgtcat aggtggtcac
     1321 gcgacgaatc atgtgcggat cgacgcgaat gccgtcattc ggaaacacgg taaacgcgga
     1381 ggtatgttcc agcagattta aatccgcagc gcccagcgta atcggcaagt atggcggcaa
     1441 gggactcgtg atgccaaatc ttcgcgccat atcgatcaca ctgttcatgc caattttttc
     1501 cgcaagcttc acggccggaa cgttgcgtga accgtccagc gcgcgccgca gggtaatcac
     1561 gccttcaaat ttttcatcat agttgtgcgg cgaatatgcc tggccgccgc tcatggtcgt
     1621 aaatggcgcg tcgagaatcg tatcgaatgg ggtgaagccc tgctccatgg cggcggcata
     1681 gacgtaaact ttgaacgagg agcccacttg ccgctgcgcc tgcatggccc ggttaaattt
     1741 tgattcgtca aaactgtagc cgccaaccat ggccttaatc tcgccggtcg gattatcgat
     1801 ggccaaaatc gcagcctgcg gtgcaacgtt ctgctccagt tccacgtggg ctacgttccc
     1861 gctgatgtcc ttgatataaa attcagcgag atcgcctgcc ttgagcaaat cggaaggaag
     1921 cttgcggccc gtccaagcaa aatccggagg cgtaagcagt gcgcgcagcg acccaatctt
     1981 aatcgtcgcc tctttctgat ccgcggcggt caccaggccc tgtacgtaat cgcctttgct
     2041 aatgggccag cgccagtcgt catcttcgta cgattccagc gtatcgcttt tatccgcgag
     2101 gatattatcg agcttcccgc gccagccgtg acggcggtca tacgcatgaa gaccgtcgtg
     2161 aatggcgcgg ttcgcggatt tttgcatgtt cacgttcagc gtggtgtaaa cacgcagccc
     2221 gcgttggtgc accatttccg tgccataggt gctctctaaa tacttgcgaa tctcttccac
     2281 aaaatagggg gccagcgtat ttcgcggaga ttgttgatgc aatccgagcg gctgcgcgat
     2341 ggcttgcttc gcctgtgctt ccgtaatttt tccgttgcgc gccatcaact cgatcaccag
     2401 attgcggcgt tgcagcgcgc gctgcgcgtg caaaatcggc gagtaactcg gcccgcgaat
     2461 catccccgcc aggagcgccg attcctgaat gtttaatttc cccaccggtt tgccaaaata
     2521 agattccgat gccgcttcaa atccataatt gccgtgggac agataaatct gattgccgta
     2581 catcgtaaaa atctgctgct tcgtataatg gcgctcgatc tgaagagcca gcagcgcttc
     2641 ctgaattttg cggcccatgt gccggtcgga ccggtccaaa aaaagcgtgc ccgccagttg
     2701 catggtgatg gtgctggcgc cttccacgac gcggtgcgag accagatcct tatatgccgc
     2761 tcccgccacg cgcgtaaaat ccacgcccca atgctcatcg aagtgttgat cctcggtcgt
     2821 gagaatcgcg tccttcagaa tctggggata ttgttcgtag gtgagcagca cgcgcctctg
     2881 cagcgcgaaa ttcccaacca cctggccgtc atccgcataa agttccgtga ccacatcggg
     2941 ctggaaatcc tcgagcgcgc gaatctgcgg aagatcgctc gagtaaacaa accacaatcc
     3001 cgccatcgtg cccagggcaa tggagccaag cagcaacaga ataaaagcaa cacgcccaac
     3061 aaccttgcga ccccgcaagt tgagggtgat cggcggaaga tctaccatga gaatgtcatt
     3121 ctaacgcaac ccgcggggca aatgcgtgga tcaggaagat actctttcgc ggcgtttctt
     3181 aatcagaaac gctccaaccg tgacggcgag agccagaacc agaagactgc tgtcgcgaat
     3241 taacgttgcg gcgcccaact tttcattgtt tccaaagcag ccgcaattaa tttccaggcc
     3301 cagaattttg gcgcgcgtca tcgcgccaat gaacacgagc agcaggccgc tggtaatcgt
     3361 gccgatcaca cgcaagccaa ttccggcgat tagaaatatt cccaacacga gctcaaacca
     3421 tggcaggatt tgcgccatca gctgcacggc ggaaacggga agcatcttgt aggagtcgat
     3481 ggccatggcg aagaagaact ggtaatcgcg caaatgccat tcgcccgcga aatgaagttt
     3541 cgcgtatgcc gcaatcagga aaattgcgcc aagcgccact cgcccgatca cgagcagcgt
     3601 tgcgggccat ggagccgggc gccgtgaaac ttctgatgaa atcgcagtgg tagccacggt
     3661 tttcttcaat tattggcgca gcagataatc aaaatagctt ttcagcagct tgtaatccac
     3721 tccgccgccg ggaagcgctt ccatcttgcc atgtgaagta atgtagatcg ttggagtctg
     3781 gtttacatta cgctgcgttc ccatcgcctg atcttttgcg atggaagcgt tgatttcatt
     3841 catgtgctgc gcttcaaatg cctcgatctt cttgagctgc gccggaggaa aataaggggc
     3901 gatgacctga tcgatatttc ctttggccac ccattcatcc tggtgatcgt aaagtgtccg
     3961 ctccatggcc tcgaattgcc cgaactccgc agccgcgttc gccagccgcg ccgcctgccg
     4021 cgcgtaagga tgaatatcca gcgggaaatc gcggtgaatg atgtacacct tgccgggaat
     4081 cacgtaatcg tcaatcactt gtttcacggt gccttcaaaa aaaccgcggc acgcggggca
     4141 ttcaaaatcc ccgaacactt ccattgtgat gggcgcgctc ggatttccaa tcgctttttg
     4201 gaaagtggga gcagcggtcg atgcgggagt tggcttcgac gcgggtgaca ctggagactt
     4261 cgtctgcggc cttgcgaaaa ccgtcgttgc cattgcacac agggcaacca gggtcacaac
     4321 tcgaatcggg cggacggtta ggaaattcac gcgacgttct ccttggagat tagatgagca
     4381 acgcattcta accccaaaac gggtgtttct gtacaggata gacgtggaaa ggaaaggtag
     4441 ggttacccgg cagtcgccag aaatgttttc aattgctatc ttttcaattg ctgtcttaag
     4501 gattgaggtt tcggtgaggg cagtcctgtt gagagcaagc cagtaaaagg gagttacgcg
     4561 aggcaattaa aagagctgga agaatagcca ggaaaacagg tcagaagatg acgcaggaac
     4621 aaactcgccg caagctactg caaacaatga agatatacac tatcaaaata cttgacaaag
     4681 atacactcaa tgtatagaat caccgcttgt aaggcactca agccagcaag tgccggccat
     4741 gagattcagg aggggagtca gcaatgagta aaattattgg tattgatctg gggacgacca
     4801 actcggtagt ttccgtcatg caaggtggag agccggtcgt gattccgaac caggaaggtg
     4861 ggcggacaac cccttcggtg gtcgcgatta ccaagacggg cgaacgcctg gttggacaag
     4921 tggcgaaacg ccaagcggtg accaatccgg aaaacacagt ttattcgatc aagcgcttta
     4981 tgggtcacag attcgatgaa gtgaccgaag agaccaagcg tgttccttat aaggtggtaa
     5041 aggggccgca tgatgacgcg cgcgtcgaaa ttttcggcaa ggcttatacc ccacccgaat
     5101 tatcggcgat gattttgcaa aaactgaaga cagccgccga ggatttcctc ggagaaaaag
     5161 taagcaaggc ggtcatcacg gttcctgcct actttaatga cagccagcgg caagcaacca
     5221 agcaagcggg cgaagtcgca ggcctcgaag ttctgcgcat catcaacgag cctacggcag
     5281 cagcactggc ctacggcttg gacaaaaaga aagacgaaac aatcgctgtc tatgacctgg
     5341 gcggtgggac gttcgatatc tcgatcctcg aagtgggcga aggcgtggtc gaagtgaagg
     5401 ctaccgctgg cgacacacac ctcggcggag acgacgtaga caatcgcgtt gtcgattggc
     5461 tgatcgcgga atttaaaaag gatcagggca tcgacgtcag caaagatcgc atggccgtcc
     5521 agcgactgaa ggaagccgcg gagaaagcca agatcgagtt gtcgcagatg caagagacgg
     5581 acatcaatct tcctttcttg accgcggatg cgtccggccc gaagcatctg caactcaagc
     5641 tgacgcgcgc gaagctcgaa cagttgatgg aagatattct gcagcgaaca attgggccca
     5701 cgaagcaggc tctatccgac gcaggcatca cgcccgacaa gatcgacgaa gtcgttctgg
     5761 ttggcggatc gacgcgtatc ccgcgcgtgc tacagatcgt gaaagatctg ttcggcggca
     5821 aggaaccgca caagggcgtt aacccggacg aagttgtggc ggtgggcgcc gcaattcaag
     5881 gcggcgtgct cggcggcgaa gtgaaggaca ttctgctcct ggatgtgacg cccctgtcac
     5941 tgggcgttga aaccctgggc ggcgtgatga ccgtgctgat ctcgaggaac acgacgatcc
     6001 cgacgcgcaa gtccgaagtg ttctcaaccg cagccgataa ccagccgtcg gtggaaatcc
     6061 acgtcctgca gggcgaacgc aaaatggcgc ccgacaatcg cacacttggc aaattccatc
     6121 tcatgggaat tccgccggca ccgcgaggca tgccccaggt ggaagtcact ttcgacatcg
     6181 acgcgaacgg catcctgaac gtctccgcaa aagaccgcgc gacgaacaag gagcagaaga
     6241 tcacgattac cgcttccacg gggcttagca aagaagaggc cgagaagatg cgatcggacg
     6301 ccgaagctca cgccgaggac gacaagaaaa aactcgcgga gatcgaggcg cgcaacattc
     6361 tcgacaaccg cgtgtatcag gtcgagaagc tgatcgcgga caatcgtgac aagctttccg
     6421 aaacggacac gaagccggtc gaagaggctg tggcagcggc gaaaactgcg ctcgcgggag
     6481 gcatcctcga acagatccaa tcggcgaccg tgacactgga aagcgcttcg cataagctgg
     6541 ctgaggttct atacaagacc gcggcggctg ccggagcaca accgggcgca ccgggttcgc
     6601 aggacggcgg accaggcgcg gctccgcata gcgggccggg tcccacgccc gaaggtgaag
     6661 tgatcgacgc cgaatatgtg gacgccgacg aaacaaagaa gccgaactaa aaatgaaaat
     6721 tgtgtgcgga ggggcatcgc tcctccgcac acaatttttg ctgttcatga attgatagga
     6781 gcgagcgttg acgacgtacg aaggaattaa gcgtatcggg ccgccgcaag cgattcagaa
     6841 attacgcgaa gatttgttca tggttcaaat cgcgctgacg gaaactccga cagccgattg
     6901 gaaaaggctg ttttacgaaa cgcagcaggc gccgccgccc gattttctgc cacgcgcggt
     6961 ggaaattatc agcaattaca ttcgctttcg ctcggaagtc cccagcgtgc aggcgaggac
     7021 agcgtggatc gaccgctgga tagaccgtgc gaatcaaaaa gaagcgagca tgggcggtcg
     7081 gctggacgaa gagcgccgaa agcgccgcga cgaacaccag cgtgagcagc aagagctcgc
     7141 agcgattaac gcgcaatggg aaaagctgta aatggaaatt gaggaaataa aggaagtaga
     7201 ggacataaag gaagtttagg aagtgaagga accgaaagaa gcgcaaggac ggttgacgag
     7261 tgatgcgccg cgaagaacat atcacgtttg aagaatcacc cttcgcaggg gctcagggca
     7321 agccagtcac tagtcaccct tcgcgggagc tcagggcaag ctagtcacga attacgattc
     7381 acgactcacg gaccaccaat ggccacaaca acaaaacaag attactacga tctcctgggc
     7441 gtctcgcgca aggcggcgca aaaggaaatt cgtgcggcct accgaaaact tgcgcgcaaa
     7501 tatcaccctg acctgaatcc cggcgataaa tcttccgaag aaaaattcaa gcaggtgcag
     7561 gaagcctacg acgttctctc cgacgcaaaa aaacgtcaga tgtacgatca attcggtttc
     7621 gacacgcctg gatccggcgg cacgcctgcg cccggaaatg gcggagacgt ccattttgat
     7681 tttggcggtt tcgattttgg cggccagggc gcaggcggcg cgggcggagg acccagcttc
     7741 aaggatttgt tcagccagtt ctttcgtggt ggtggagcta cgaccgaagc ccccacacgt
     7801 gccgaacctg gttcgagcct cgaatatcag atcgaaatca gtttctggga agctgttcgc
     7861 ggcacggtaa aaaaactgac catctcgcgc ctcgatgtgt gcaccgagtg ccgcggcact
     7921 ggaaccgtcg gccagccgca gacatgcacg gcgtgcggcg gttcgggtca ggtgacgcaa
     7981 actagtggaa agatgcgctt caatctgacc tgctcgcgct gcggcggttc gggacgcgtg
     8041 cgaaatatct gccgcgtttg cggcggcgaa ggccgcgtgc gtcgcgcgga caccatcgac
     8101 attcgaattc ccgcgggagt gcaaaccggg tcgcgcgtgc gcgtcccagg acgcggaaat
     8161 gcgggatcag gcggagagcc gcccggcgat ctgtacatca tcacggacgt ccaaccgcac
     8221 ccatttttcg atcgccgcgg cgacgacatt tatatttccg ttcccatcac cgtgaccgaa
     8281 gcggtgctcg gcgcaaaaat cgaagtgccc acaatcgatg gcccttcgcg gcctctgcgc
     8341 attcctccgg gaaccaatag cgggcaaaag ttgcggctgc gcgaaaaggg tgtgccttct
     8401 gttcgcacgc cggggcaccg cggagaccag tacatcgaat tgcaagtggt tgtcccaaag
     8461 cccgtggatg agcgcgtgaa agaacttctt cgcgagctgg aaaaaatcgc gccggaaaat
     8521 ccacgcaaag acctttttga ccgggccaag gtctaaggag cgcagccatg gcgcgcgtaa
     8581 ccacaaaacg aaaaaaaact cgtgcaggtt acatgatttc ggccgtagcg gagttgtaca
     8641 agctccatcc gcaaacgctg cggctttacg agcgcgtggg cttgctgaaa ccttcacgct
     8701 cggacggcaa cacacgcctg tacaccgatt ccgatctcga acgtttggaa gttatcctta
     8761 cactgacgcg cgaaatgggc gtcaacctcg cgggcatcga aattatttta aacatgcgcg
     8821 aaaaaatggc cgaaatgcag cagcaaatgg aggcttttac ttcgtttgtg caacaggaac
     8881 tcgcgcgcgg catgcgcgcg gcaggctcaa gacagcaggg caatgccctg gcgcgtgtcc
     8941 ccgtgccgaa agtgattcga gtgcataagt aaagtgattc gtgaatcgtg atttgtgact
     9001 ggcttgccct gagctcccgc gaagggtgac tggtgactgg tgactggtga ctggtgactg
     9061 gtgactgaaa aatcatgatt tgtgctgcat cgcttgtcaa cctcatcctt tacttggtgc
     9121 tgtcctaatt taaaaagaat tcgcgtggtg cggttcctta ggaggtcggg acttcagtcc
     9181 cgacgagaag cttggtttga tcggggtatt aatccctgaa gcttcagggg ctgaagcccg
     9241 cgacgaaagg caatccaaat ggccgagttg aaactcggcc ctcctaaatt ctagacattt
     9301 tcaaattagg acattgccct ttacttcctc aatttcctct actacctcag cctcctccac
     9361 ttccttttcc acacgcgcga acgctttcca caggcttgga catcttgcgc aactacatcg
     9421 gcgcgcctat gctcaagcta tggcacaacc tgattgcccg atatgcagtg gctccggctg
     9481 gaaaactgtc gagcgcgtat cggaagaaga aaatctgaaa cgtgtctcgt gggaaaaacc
     9541 ctacgaaggc accaacatca ccaggcgcgt atgggccgtg ccctgcgatt gcacgggcaa
     9601 agaccgcgcc acgcgggtgt tgaaccgcgc gcgcattccc gaccgctacc aaaactgcga
     9661 cttcgaaagt ttcgagaaag atgtttatga cattggcatc cacggtcccg acgcaccagc
     9721 atggaaccgc agcctcgaac aagccaaagt tgtaacagaa ggttttgccc gcaattatcc
     9781 aacaggcgcg gagaccggct tactcttaat gggcacgtac gggaccggta aaacgcatct
     9841 tgcggtcgcc gctcttcggc aaattatttt
//
LOCUS       DASZMB010000175         3669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_748464, whole genome shotgun sequence.
ACCESSION   DASZMB010000175 DASZMB010000000
VERSION     DASZMB010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748464"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="VG272_07940"
     CDS             <1..1344
                     /locus_tag="VG272_07940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HWF13649.1"
                     /translation="RRPEYFKGAQLRVGIIGCGYVGLPLGLRFAEAGHRVTGFDTDPA
                     KVDKLNRGQSYIQHIPQNKIQQFVQSKHFGATTDFSRLAEVDAIIICVPTPLDQRREP
                     DLSYVTDTAQAIRPHLQRGQLVVLESTTYPGTTEELVLPVLEETGMKCPVAHGPDNDQ
                     ISTDFFLAFSPEREDPGNKQYGLAQIPKVVGGVNPPSGRAACSLYAQIVAKVIPVSST
                     QAAEMTKLLENIFRCVNIALVNELKMLGMRMGIDIWEVIDAAATKPFGFMPFYPGPGL
                     GGHCIPVDPFYLSWKAREYDFATRFIELAGEVNTAMPYHVVDSVAAALNERQKSLKGS
                     RIMVLGVAYKKDVDDLRESPSLKIIEQLVLRGAKVDYNDPHFAQIHKLRHYNFEGMKS
                     VPIDAKTLASYDCVLIATDHTAYDYPMIVESSQLVVDTRNSTRRIARNRNKIVLV"
     gene            complement(1396..2265)
                     /locus_tag="VG272_07945"
     CDS             complement(1396..2265)
                     /locus_tag="VG272_07945"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HWF13650.1"
                     /translation="MLERIGATRLRRFGRNRLLSKLIRIHSFTIGASLLAGVLALASM
                     GFAQQPKPSKKHAKIAAAPATPAAIPVPFRSGENLEYRVLLSKYALNAAKIEIMVVEQ
                     RNFFGHSAWHFRASAHTMDTTRTLFPLDDQFDSYTSAANLFSLQFEMYLHEQGKEQTN
                     LFRMTSEGDPAPTDATAVRVVPGTRDAIGFLYDLRAADWQRTPELHAPIFDGRRLYDA
                     IARVDTPQGTVTVPAGTFPAFRVSIRLFDHGKEVTETKLWVWFTKEAARTPVLVEAEI
                     PFGTARIELTHLP"
     gene            2448..3386
                     /locus_tag="VG272_07950"
     CDS             2448..3386
                     /locus_tag="VG272_07950"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWF13651.1"
                     /translation="MRYLITGGAGFIGSHTVDELVRRGHDVTVVDDLSTGKPANLSQV
                     KSKIKFVQESIVNLEALREVFRDVDCVIHLAAQTSVPRSVKDPIETNLINVTGTLNVL
                     MAAREAKVKRVVFACTCAVYGKTSTLPIAENAALDPISPYGVSKQFGEAYGRVFQDLY
                     GLEFVSLRYFNVFGPRQDPGSPYSGVLSVFNENLLRGTAPTVYGDGEQSRDFVYVENV
                     VQANLLAAEIKRGPFQAINVGTGIRHTLNRTLALLEQITGHPAQANYAPARDGDIRES
                     QADISLAKAALGYHPRVTFEEGLKRTWQWFSANERA"
     gene            complement(3427..>3669)
                     /locus_tag="VG272_07955"
     CDS             complement(3427..>3669)
                     /locus_tag="VG272_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HWF13652.1"
                     /translation="PDPMGVELILRQFGAAPAQTMFIGDSDVDVQTARNSGTWVCGVT
                     YGFGSHRLTDYPPDLLVDNLLELVPYLSSSPLERSR"
BASE COUNT          880 a          989 c         1004 g          796 t
ORIGIN      
        1 cgcagaccag agtattttaa gggtgctcaa ttacgtgttg gaattatcgg gtgtggatat
       61 gtggggctgc cgctgggact gcggtttgct gaagccgggc atcgtgtaac cggatttgac
      121 acagatccgg caaaagtcga caaattaaat cgcggacaat cctatatcca gcacattccg
      181 caaaataaga tccaacaatt tgtgcagtcg aaacattttg gcgcgaccac cgatttttcg
      241 cgcctggctg aagtggacgc aatcatcatc tgcgtgccga cgccgctcga tcagcgccgc
      301 gagcccgatc tttcctatgt tacggacaca gcccaagcca ttcgcccgca cctgcagcgc
      361 ggccagttgg ttgtgctgga atccacgact taccccggaa ccaccgaaga actcgtgctg
      421 cccgtgctcg aagaaactgg aatgaagtgc ccggtagcac acggacctga caacgaccag
      481 ataagcacgg atttcttttt ggcattttcg ccggagcgcg aagatcccgg aaacaaacaa
      541 tatggactgg cgcaaattcc caaggtcgtc ggcggagtaa atcctccctc cggccgcgcc
      601 gcctgctcgc tgtacgcgca aatcgtggcg aaagttattc cggtgagttc cactcaggcc
      661 gcggaaatga ccaagctgct cgaaaatatt ttccgatgcg taaacatcgc cctcgtcaat
      721 gaattaaaaa tgctgggcat gcgcatggga atcgacatct gggaagtgat cgacgctgcg
      781 gcgacgaagc ctttcggctt catgcccttt tatccagggc cgggcctcgg cgggcattgc
      841 atccccgtgg atccttttta tctgtcgtgg aaggcgcgcg aatacgattt cgcgactcgc
      901 ttcattgagc tggccggcga agtcaatact gcgatgccct accatgtggt ggattccgtt
      961 gcggcagcct tgaacgaaag acaaaaatcg ttgaagggtt cccgcataat ggttttaggc
     1021 gtggcctaca agaaggacgt ggacgatttg cgcgagtcgc cctcactgaa aattattgaa
     1081 caattagtgc tgcgcggcgc aaaggtggat tacaacgatc cgcacttcgc gcagattcac
     1141 aagctgcgcc actacaattt cgaaggaatg aaatccgttc ccatcgacgc caagacactg
     1201 gcctcctacg attgcgtcct gattgccacc gatcacactg cttacgatta cccgatgatc
     1261 gtcgaatctt cgcagctggt agtggatacg cgcaactcta cgcggcgcat cgcgcgcaat
     1321 cgcaacaaga tcgttctggt ctaaaacatc caggaccaat caaaaaatag ttttacagga
     1381 atttcgagat cccgttcagg gcaaatgcgt gagttcaatt cgcgcggtgc cgaacgggat
     1441 ttcggcttct acgagcactg gcgtgcgggc cgcttccttc gtaaaccaca cccacagctt
     1501 cgtctccgtg acttcttttc cgtgatcaaa taaccgaatc gagacgcgaa atgcggggaa
     1561 agttcctgca gggaccgtca cggttccttg cggcgtatcg acgcgcgcaa tggcatcata
     1621 gagccgtctg ccatcaaaaa taggggcatg caattccggc gtacgctgcc aatccgcggc
     1681 gcgcaaatcg taaagaaatc cgatcgcatc gcgcgttccg ggaaccacgc gtacagccgt
     1741 ggcatcggtt ggggctggat cgccttccga cgtcatccga aataaattgg tttgctcttt
     1801 gccctgctcg tgaaggtaca tctcaaattg caaagaaaat aagttggcag cggacgtgta
     1861 agaatcgaat tgatcgtcca gcggaaaaag agtgcgcgtc gtatccatgg tgtgcgccga
     1921 agcacggaaa tgccatgcgg aatggccgaa gaaattacgc tgctcgacca ccatgatttc
     1981 aattttcgcc gcattcagag catacttcga cagcaaaacg cggtactcca gattttctcc
     2041 cgatcgaaac ggaacgggaa tcgccgcggg cgtcgcgggc gctgcggcaa ttttggcgtg
     2101 tttcttggaa ggtttgggct gttgtgcgaa tcccatggac gcgagcgcaa gcaccccggc
     2161 aagcagggac gcgccgatcg taaaggaatg aattcgtatg agtttgctca gcaatcgatt
     2221 tcgcccgaag cggcgtagcc gggtcgcgcc aatcctttcc agcatgctaa cgtagacgcc
     2281 gcaaatccgg ccggcaggca cgctctattc agggtacaga aaaaaaggct cagagaccag
     2341 aattgtcgag cccggtgttt agatgcggga accggccgcc gcgtggttcg tctgcgcacg
     2401 caattgcgca acgcgtacgc cgcgccacca atttcgaggg ggaatggatg cgttatctga
     2461 tcacgggagg cgcgggcttc atcggttccc atacggtgga tgaattagtg cgccgcggcc
     2521 atgacgtaac cgtcgtggat gatttatcca ccggcaaacc cgcaaacctc tcccaggtaa
     2581 aatcgaaaat taaattcgtt caggaaagca ttgtgaattt ggaagcgttg cgcgaagtct
     2641 tccgcgacgt ggactgcgtc attcatctag cggctcagac ttccgtgccg cgttccgtga
     2701 aagacccaat cgaaacaaat ctcatcaacg taactggcac gttgaatgtt ctgatggcgg
     2761 cacgggaagc aaaagtaaaa cgcgtggtat ttgcgtgcac ttgcgcggtc tacggaaaaa
     2821 cctcaacgct gcccattgcc gaaaacgccg cactcgatcc catttcgccc tacggtgttt
     2881 ccaaacagtt cggcgaggcc tacggacgtg tgtttcagga tttatacggc cttgaatttg
     2941 tctcgctgcg ctattttaat gttttcgggc cgcggcagga tccgggttct ccttattccg
     3001 gcgtgctctc cgtattcaac gaaaatctgc tgagaggaac agcgcccact gtttatgggg
     3061 atggcgagca atcacgcgat ttcgtctacg tggaaaacgt agtgcaagca aatttgctcg
     3121 ccgcggaaat aaagaggggc ccattccagg caattaacgt cggcacggga atccgccaca
     3181 ccctgaatcg gacattagca ttactagaac aaattactgg ccatcctgct caagcaaact
     3241 atgcgcctgc ccgcgatggc gatatccggg aatcgcaggc ggacatcagc ctggcgaaag
     3301 cggcgctcgg ctaccatccg cgtgtcactt ttgaagaagg attgaagcgc acgtggcagt
     3361 ggttctctgc aaatgaacgc gcttagctcc tgaggtaaac gggcttcaga tctaattcga
     3421 aattttttag cgggaacgtt ccagcgggct gctggacaaa taagggacaa gttccaggag
     3481 attgtcaaca agcagatcgg gtggataatc cgtgagccgg tggctgccga atccgtaggt
     3541 tacgccgcaa acccacgtgc cggagttgcg cgcggtttgc acgtcgacat cggaatcgcc
     3601 aataaacatg gtttgagcag gagcggcgcc gaattgtcgc aaaattaatt ccacgcccat
     3661 tgggtccgg
//
LOCUS       DASZMB010000176        14402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_5125623, whole genome shotgun sequence.
ACCESSION   DASZMB010000176 DASZMB010000000
VERSION     DASZMB010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 14402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14402
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5125623"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..1240)
                     /gene="ruvB"
                     /locus_tag="VG272_07960"
     CDS             complement(185..1240)
                     /gene="ruvB"
                     /locus_tag="VG272_07960"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF000868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HWF13653.1"
                     /translation="MSHGTGSEPKMDPAGHRIVTGAMLDEDARIDASVRPQRFADYIG
                     QTRVKENIEIAIQAARSRGEALDHVLLYGPPGLGKTTLAQIIASELGVTIKTSSGPLL
                     ERKGDLTAILTSMEQREVFFLDEIHRLAPAIEEVLYPAMEDYRIDLVIGQGAGARIHP
                     FILNRFTLIGATTRAGLVTAPLRSRFGIVHRLEFYTPEDLVTIIRRSASILGIVVDDE
                     GAYEMARRSRGTPRVANRLLRRARDYAQVRAQGHITLAVAKESLQMLGVDEHGLDEVD
                     RNLMLTILQKYAGGPVGLSTLAASISEEEDAIEEIYEPYLMQLGLLDRTPRGRVATAL
                     AYKHFGLEQPRRQGGLF"
     gene            complement(1242..1865)
                     /gene="ruvA"
                     /locus_tag="VG272_07965"
     CDS             complement(1242..1865)
                     /gene="ruvA"
                     /locus_tag="VG272_07965"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HWF13654.1"
                     /translation="MIAQLRGTLGDKRPNQVLVDVGGVGYLVHIPLSTFYALGDLHAN
                     VTLLIHTQVREDAISLYGFLSAREKHLFELLISASGVGPSLALKILSGMSVDDLVPAV
                     RAGDLPRLTRIPGVGRKTAERIIVELRDKLAAEEFPEDARKPVSTTGTTGDVVSALLN
                     LGYDQHAAEQAVERAGKKGASETFEALLRSALQELTAPSQNSARAAR"
     gene            complement(1941..2375)
                     /locus_tag="VG272_07970"
     CDS             complement(1941..2375)
                     /locus_tag="VG272_07970"
                     /inference="COORDINATES: protein motif:HMM:NF016334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HWF13655.1"
                     /translation="MTPNQKQVHQFLSEFSRWAASQPDILAVALLGSYSRNEATGNSD
                     VDLVIVASEPKIYVQDTRWAQYFGTISRQQLENYGKVISLRVWYSGGHEVEYGFTDET
                     WCALPLDEGTKKVISDGMQILSERGPILTRLKRPKTVAGDLK"
     gene            complement(2523..2798)
                     /locus_tag="VG272_07975"
     CDS             complement(2523..2798)
                     /locus_tag="VG272_07975"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HWF13656.1"
                     /translation="MLIGLVIGDVVATQKVPSHEGRKILMVQPIQLDGSDRGDPILAL
                     DAVGAGIGERVLLTTEGFSAMTAVGRPNSPIDAAVIGVIDSIDLRDK"
     gene            complement(2880..3152)
                     /locus_tag="VG272_07980"
     CDS             complement(2880..3152)
                     /locus_tag="VG272_07980"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HWF13657.1"
                     /translation="MLLGRIIGNVVCTMKDPALIGQKLLIVQPLDRKGRDKGRPVVAL
                     DAVGAGTGETIYWCRGKEASFPFAHADLPTEATIVGIVDSVNIPAN"
     gene            complement(3153..3491)
                     /locus_tag="VG272_07985"
     CDS             complement(3153..3491)
                     /locus_tag="VG272_07985"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HWF13658.1"
                     /translation="MKFESEVDPAYMILARIVGTVVATRKDARLEGKKLLVVKPISPQ
                     GDDEPGYLIAVDTVGSGFSEKVLVVTGSSARNAAECKDRPVDAAIVGIVDEVVLDALH
                     EAELADARKK"
     gene            complement(3611..3907)
                     /gene="eutM"
                     /locus_tag="VG272_07990"
     CDS             complement(3611..3907)
                     /gene="eutM"
                     /locus_tag="VG272_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HWF13659.1"
                     /translation="MGEALGMIETRGLIAMIEASDAMVKAAKVTLVGWEKIGSGYVTA
                     MVRGDVAAVKAATDAGAAAARRVGELVSVHVIPRPHQSLEDVLPIGKTAVAAGD"
     gene            complement(4003..4821)
                     /locus_tag="VG272_07995"
     CDS             complement(4003..4821)
                     /locus_tag="VG272_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HWF13660.1"
                     /translation="MNDDFIANGNVKSEDEHRRDICTVGRWIHSRSFVVATDGNISVR
                     LDSQRVLTSPTGMSKGMMVPDDLVITDLRGRKLSGRREASSELAMHLLIYNRRPEVYS
                     VVHAHPPTATAHAAAGIPLNKALLSELIIALGCIPVAPYGTPGTQELSDALEPMVQHY
                     DAILLANHGVVTCGPDLLTAFFRMETLEHFAQVSLMTEMLGKQVLLSGRDVEKLLVAR
                     ARYGTTTAAPMLPGCPVTRDNAGSDDHRLKRISITREELEGLIEEAVRKDRSLR"
     gene            complement(4940..6211)
                     /locus_tag="VG272_08000"
     CDS             complement(4940..6211)
                     /locus_tag="VG272_08000"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HWF13661.1"
                     /translation="MATLVGRDEFNVYESADARFEAAARKLGLEEGVYRFLKYPNKEI
                     TVYIPVIRDNGHLEVFIGYRVHHSIVRGPGKGGVRFAPNVTLDEVRALASWMTWKCAV
                     VNIPFGGAKGGVICDPTKLSVGELERITRRYTAELIEWFGPERDVPAPDLGTNEQTMA
                     WIMDTYSMHVRHTETAVVTGKPVDLGGSRGRHEATGRGVLFNCDRALAKFRMLRVNTR
                     VIIQGFGNVGSMAAKLLHDAGYKIVGLADVHGALYNENGFDVPKVIDWVNVQRQPLPD
                     FSGGGAKMDASEILFQPAEVIIPAAVENQITSKNAHRLQARILCEGANGPSTAMADEI
                     VDRKGIFVIPDILANAGGVTVSYFEWVQDRQGYFWSEKEVNDRLKDVIERSFEEVVRY
                     AETHQVNNRVAAYMLGIDRVAFALKERGIYA"
     gene            complement(6380..7423)
                     /locus_tag="VG272_08005"
     CDS             complement(6380..7423)
                     /locus_tag="VG272_08005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HWF13662.1"
                     /translation="MKKSTRQRGTGHGGVRVVAIGGGTGLSMLLRGLKNFIARRRHES
                     ERHPISDLAAIVTVTDDGGSSGRLRREYRVLPPGDIRNCMVALSQDEALLGRLFQYRF
                     HAGRGLRGHSFGNLFLTALSHVTGDFSEAVRVSGEVLAIRGRIFPTTLENVSLEAVMA
                     DGKIVSGETRIAKCRKKIRRLNLRPRHVRPLPEVLEAIHKADLILIGPGSLYTSILPN
                     LLVSGVAEAIEKSAAIRIYVANLMTQPGETDGFSLADHIRVIYSHTRRKIFDWVVASN
                     QPISLEVARRYSRRGAESVRVDLPDLQRMGVRCLLDNLLEEHGVVRHDAQRLSQLLIE
                     EFIERQPPSTKPH"
     gene            complement(7466..7972)
                     /locus_tag="VG272_08010"
     CDS             complement(7466..7972)
                     /locus_tag="VG272_08010"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF13663.1"
                     /translation="MVEPQFEQQLKIYEQAVQHFHQQKFVKAKELLEKVEAGPSKELA
                     DRARVHLRIVEQRMAKQAAPSVRTPEEHYQSGVAMMNLGRWDEAREHLLRAKKLAPKA
                     DYVFYAMAALDCLTGEAESAMENLKIAIQLRPENRYHARNDEDFAFLQEDPRFTELLY
                     PEREASGA"
     gene            complement(8204..8776)
                     /locus_tag="VG272_08015"
     CDS             complement(8204..8776)
                     /locus_tag="VG272_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWF13664.1"
                     /translation="MNYMKGFVFGVLVGVVLLAGSVYYYFTSGMAPAAVADPPMMMEK
                     KMANKSLDAHIEKANVPAPPIQPSEDNYVAGAKLYKTQCAVCHGLPNQPAPIALNMYP
                     NATLMFKGKGVTDDPPQESFWKIKNGIRLTGMPTFTGILDDNQMWQVSLFVANVDKLP
                     DSAKKLLVPDSSPSAAMPATGMPSTPATKK"
     gene            9092..9679
                     /locus_tag="VG272_08020"
     CDS             9092..9679
                     /locus_tag="VG272_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HWF13665.1"
                     /translation="MPFINFPAREINCKLVYYGPGLGGKTANLQWIYDHTGSAQKGKM
                     ISLATETDRTLFFDFLPIDLGTVRGFKTRIHLYTVPGQVFYEASRKLILKGADGVVFV
                     ADSQEERLDANLETLENLREHLKEHKLDFQTIPYVLQLNKRDLPGVLSIEELTRQLQV
                     KGEPIMEAVAITGQGVFETLREVAKLVLAELKKGT"
     gene            9858..10766
                     /locus_tag="VG272_08025"
     CDS             9858..10766
                     /locus_tag="VG272_08025"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWF13666.1"
                     /translation="MNSMLPTIPDKQQLPSSERTLQEALEDLQKLERRDWWLWSMAVV
                     VMLLLTFAVFSMSFPGIIKVDDPLFELSLSREVRGLVGLVLIFNAYTIYQQIMVKRLR
                     RDYSSKIGEMRTLQVRAEELERLAQVDPLTGLVNRRVAEEHLASEASRCKRNGHPLTV
                     VCFDLNNFKQINDTYGHLAGDLVLKHFGRRLQSAVRKSDTAARMGGDEFLIVLPDCTT
                     SQVQILIARLRPMDIEWGGVKIPICFSAGWVGYEKSETPEQLLERADRILYAEKRSGK
                     TLEMEANANARRETQAKAELSAATVS"
     gene            10917..11258
                     /locus_tag="VG272_08030"
     CDS             10917..11258
                     /locus_tag="VG272_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13667.1"
                     /translation="MNEITTTRRRSQRLFLRVPVMVEGSEPNKPPFREETYTVVLNAH
                     GTLLEMRAAIETGRTVTLKNLRTNEQIECTVKLVTPSDGGKFNVALEFRKPNASFWQI
                     SFPPEDWAPAR"
     gene            complement(11604..13973)
                     /locus_tag="VG272_08035"
     CDS             complement(11604..13973)
                     /locus_tag="VG272_08035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF13668.1"
                     /translation="MSQATTNAALTRLWNMSAPLHPLLIENSSPEAKRNIDELERAGF
                     LCKPQIIGTRTEFLHHISRFPFDVVLSDYQLPGWTGLDAFSAMRRAGRDVPFILVTET
                     LGEEVAVECIKQGITDYVLKDHLARLPVVVARALEERALRDARQLMIQALRQSEGNSL
                     FLFAKNPLPMWVFEMETLQFLQVNDAALRHYGFTRSEFLQMNTGALHPAEEISKLLGA
                     IRDTKLHERLSGEWRHRKKDASLIDVEMFLQKMDYCGMVAGLVVALDITERKRAEEEK
                     QKFFTLVEYSRDFIAVADLQDNVEYVNPAGRAMLGIPSGHSIKGTHSLDYVIPADLPL
                     VCDTILPAIRTIGHWEGELQFRHLQTGRALPMDFVGFQVKDQKTGEPRFVATVSRDVT
                     ERRALERQLQQAQKFEAFGQLAGGIAHDFNNVIGAILGWAEMGEEQSTAGNAVLRNYF
                     KKIHIQCDRVTALVQQLLAFARRQILEPINLSLNQTVRDVMSLLDKLIEKDIEIKSNL
                     ADDLLPVRADPTQIEQMLINLCINSRDAMPKGGTITIVTHNANFSEEDARNVVGLQPG
                     RFAALQVSDTGMGMDQATRERIFEPFFTTKVMGKGTGLGLATVYGIVKQHNGFIQVES
                     EPGRGSTFRILLPINEVPAKTEFRPLVFDDLPVRGGNETILLADDHDGICEMAYSVLI
                     AKGYQVLLAHDGEEAIQIFTMHRDKISLVLLDVIMPRRSGPEVFAVIKELNPQISVVF
                     ATGYSNETAVLADLVEHGVAILRKPYSPSVLCRRVREVLDSVPAVSSSG"
BASE COUNT         3403 a         3915 c         3677 g         3407 t
ORIGIN      
        1 ttccgttttc cattgaaaga atgaaaccac ggatacacgc ggattaacac ggataaaaaa
       61 tagattattc aagagcacgt tggaaaattg ggattgaagg cgtttcattg tttcattaag
      121 gaatagttct gattttattt cttagaatct attcgtgttg atccgtgtat atccgtggtt
      181 ccaattaaaa aaggccgccc tggcggcgcg gttgttcgag gccaaaatgt ttgtaggcga
      241 gcgccgtggc gacacgcccg cgcggcgtgc ggtcgagcag tcctaactgc atgagatacg
      301 gctcatatat ttcctcgatc gcatcttctt cttcgctgat ggaagccgca agggtgctca
      361 gtcccacagg accgcctgca tacttttgca aaatggtgag catcaggttg cgatccactt
      421 catcgaggcc atgctcatcc acgcccagca tttgcagcga ttccttggca acggcgaggg
      481 tgatgtgccc ttgcgcgcgc acctgggcgt aatcgcgggc gcggcgcagc aggcgattcg
      541 ccacgcgggg cgttccccgg ctgcgtcgag ccatctcgta cgcgccttcg tcatcgacga
      601 cgattcccag gatagaggcc gagcggcgaa tgatcgtaac caaatcttcc ggcgtataaa
      661 attcgagccg atgaacgatg ccaaatcgcg aacgcaacgg cgcggtcaca aggccagcgc
      721 gagtggtcgc gccaatcaag gtaaaacgat tcaagatgaa gggatgaatg cgcgcgcctg
      781 cgccctggcc gatcaccaaa tcaattcgat aatcttccat cgcaggatat aagacttcct
      841 cgattgcagg cgcgagccgg tgaatttcat ccagaaaaaa tacttcgcgc tgctccatgg
      901 acgtgagaat cgcagtgagg tcgcctttgc gctcgagcag gggcccggaa ctggtcttga
      961 tcgtgacgcc caattcgctg gcaatgattt gcgcgagagt tgttttgccc agtccgggcg
     1021 ggccataaag caaaacatgg tcgagcgcct cgccgcggct gcgtgccgcc tgaatggcaa
     1081 tctcaatatt ttctttcacg cgagtctggc cgatgtaatc ggcgaagcgt tgcgggcgca
     1141 cgctcgcgtc aatgcgcgcg tcttcatcca gcattgcgcc cgtaacgatg cgatgtccgg
     1201 ctggatccat tttcggttca ctacctgttc cgtggctcat gctatcgggc tgcccttgcg
     1261 gaattttgtg acggcgccgt cagttcctgc aatgcgctgc gcaaaagagc ctcaaatgtt
     1321 tcggaagctc cctttttgcc ggcgcgctcg accgcttgct cggcggcatg ctgatcgtag
     1381 ccaagattga gtagtgcgga aaccacatcg ccagttgtgc cggtggttga aaccggcttg
     1441 cgcgcgtctt ccggaaattc ttcggcggca agcttatcgc gcaactccac gatgatgcgc
     1501 tccgccgttt ttcggccgac gccaggaatg cgagtgagac gcggcagatc gcctgcgcga
     1561 accgctggca cgagatcatc cacgctcata ccggaaagaa tcttgagcgc cagcgacgga
     1621 cctacgccag acgcggaaat aagcagctcg aacaaatgtt tttcgcgcgc gctcaggaaa
     1681 ccgtagaggg agatggcatc ttcgcgcact tgcgtatgga tcaacagggt aacgttggca
     1741 tgaagatcgc ccagcgcgta aaaagtcgat agtggaatgt ggacgaggta gcccacgccg
     1801 cccacgtcca ccagcacctg attcggtctc ttgtcgccca gcgttccgcg tagctgcgca
     1861 atcatcttgt gtgcctgccc gcccgcgatt atggcggcgc gcccaccgag tgtcaacgcg
     1921 atggaatctt tggtagtggg ctactttaaa tctccggcaa ccgttttcgg tctcttcaac
     1981 cgggtcaata ttggcccacg ttcagaaaga atttgcatgc catcggagat gaccttcttg
     2041 gtgccttcat cgaggggcaa agcacaccac gtttcgtccg taaacccata ctcaacttca
     2101 tgaccgcccg aataccacac ccgtaaagaa atgaccttgc catagttctc taattgctgg
     2161 cggctgattg ttccaaagta ttgcgcccat cgtgtgtcct gcacatatat ctttggttcg
     2221 ctggcaacga tgaccaagtc tacgtccgaa ttgcctgtgg cctcgtttcg ggaatatgag
     2281 ccgagcagag cgacggccag aatgtctggt tgactggcgg cccatcggct gaactcgctt
     2341 aaaaactgat ggacttgctt ctgattagga gtcaatcgga cctcgacagg cttccagtat
     2401 agtcctcaaa gtagcccact gacgaatctt tggactcacc gcgtgcggca catgatccga
     2461 atatgatcta gacaggaatt ttttcgccta tatcgatttg ttccagcaat tgtgcggcaa
     2521 agttatttgt cgcgcaggtc tatcgaatcg ataacgccga ttactgcggc atcgattggg
     2581 gagttggggc ggccgacagc cgtcatggcg gagaaacctt ccgtggtgag taaaacgcgc
     2641 tcgccgatgc ccgcgcctac cgcgtccaaa gcaaggatgg gatcgccgcg gtccgagccg
     2701 tcgagttgaa tcggctgaac catcagaatc ttgcgccctt catgcgaggg cactttttgc
     2761 gtggcaacca catcaccgat gacgagacca atcagcatga gaagaagagt agaacccttt
     2821 caaaattcaa aaaagcaaat ttcaaaatcg tatgaatggg agtagataaa aaataatttc
     2881 taatttgccg ggatattcac ggaatcgacg ataccgacga tggtggcttc cgtgggcaaa
     2941 tctgcgtgtg cgaagggaaa gctcgcttcc ttgccgcggc accagtaaat cgtttcgcct
     3001 gtgccggcgc cgaccgcatc cagcgcaacc acggggcggc ccttgtcgcg gcctttacgg
     3061 tccagaggct gaacgatgag gagcttctgc ccgatcagtg cgggatcttt catcgtgcag
     3121 acaacgtttc cgataatgcg tccaaggagc atttattttt ttcgcgcgtc ggccagttcc
     3181 gcctcatgca atgcgtcgag caccacttcg tcaacgatgc ccacgatggc agcgtccacg
     3241 gggcggtcct tgcactccgc ggcgttgcgc gcggaacttc ccgttaccac caacactttt
     3301 tcgctgaatc ctgaaccgac cgtgtccaca gcgatcaggt agcccggctc atcatcgcct
     3361 tggggagaaa tcggctttac gacaagaagc tttttgcctt cgaggcgcgc atctttgcgc
     3421 gttgcaacca cggttccgac aatgcgggcg agaatcatgt aagcaggatc aacctcagat
     3481 tcaaatttca aaaacttttt tccagacagc aaaattaatc acagcagcaa gcgtcacgca
     3541 ttcggcttcc tacatcaaga tttcgctgcg cgccgcgcca ggaacacgct cacactacaa
     3601 gtctggctgc ctagtcgcca gcggcaacgg cggtcttgcc aatgggcagc acatcttcca
     3661 gcgactgatg cgggcgcgga atgacgtgaa cggaaaccaa ctcacccacg cgccgcgctg
     3721 ccgcggctcc ggcgtcggta gcggccttta ccgcggcaac atcgccgcga accatggccg
     3781 tgacgtagcc tgaaccaatt ttttcccagc caacgagcgt gaccttggcc gctttgacca
     3841 tcgcgtccga ggcttcaatc atcgcgatca agccgcgggt ctcgatcatg ccgagtgctt
     3901 cgcccatgct ttttcctccg tggccggcat ttgaattttt tgccggtcca cctgttgaaa
     3961 aacggtatgg cctgaaaaat taccgctgcc gcacctgcgc gcttatcgca gcgagcgatc
     4021 cttacgcact gcttcttcga tcagcccttc cagttcttcg cgggtaatcg aaattcgttt
     4081 caagcggtga tcgtcactcc ctgcgttgtc gcgggtgact gggcagccgg gaagcatagg
     4141 agcagcggtc gtcgttccgt aacgtgcgcg cgcgacgagc aatttttcca catcgcgccc
     4201 agaaagcaag acttgtttgc ccaacatctc cgtcatcaga gaaacctgcg cgaaatgttc
     4261 gagcgtttcc atccgaaaaa atgcggtcaa caaatcggga ccacacgtca cgacgccgtg
     4321 gttggccagc aaaatcgcgt cgtagtgctg caccatgggt tcgagcgcgt cggaaagttc
     4381 ttgcgtgccg ggagttccat agggcgccac ggggatacag ccgagcgcga taatcaattc
     4441 gctcaggagc gccttattca agggaatgcc cgctgcggca tgcgccgtcg ctgtcggcgg
     4501 gtgcgcgtga accacagaat aaacttccgg tcggcgatta taaatcaaga ggtgcatggc
     4561 cagttccgag gaagcttcgc ggcggccaga gagtttgcgg ccgcgcaaat cagtgatcac
     4621 caagtcatcc ggaaccatca tgcctttgct cataccagtc ggcgaggtga gcacgcgctg
     4681 cgaatccaga cgcacggaaa tatttccatc ggtcgcgacg acaaacgagc gggagtgaat
     4741 ccatcgcccc accgtgcaaa tatcgcggcg gtgttcgtcc tcggatttta cgttgccatt
     4801 agcaatgaaa tcgtcattca tcggctgcgg gaaattgctt acatcctctc tgttaaatcc
     4861 gacaaagcct ataaaagaaa ccttgggtca acgcctgaat ccaaatccgc tccagacgcg
     4921 cggcgatata gtacatccgt caggcgtaaa ttccacgctc cttcagcgca aaagcaacgc
     4981 gatcgattcc aagcatgtag gcggcgacac gattattaac ctggtgcgtc tccgcatagc
     5041 ggaccacttc ctcaaaactg cgctcaatta catctttcag gcggtcatta acttcctttt
     5101 cgctccagaa atatccctgg cggtcttgca cccactcaaa atagctgacc gtcacgccgc
     5161 ctgcattcgc cagaatgtcc gggatcacga agatgccttt gcgatccaca atttcgtccg
     5221 ccatcgctgt actcgggccg tttgcacctt cgcacaagat gcgagcttgc agcctgtgcg
     5281 cgttcttgga agtgatctga ttttccacag ccgcggggat aatgacttcg gcaggctgga
     5341 aaagaatttc gcttgcatcc atttttgcgc cgccaccgga aaaatcagga agcggctgcc
     5401 gctgcacatt cacccaatcg attactttgg gaacgtcaaa tccattttcg ttatagagcg
     5461 cgccatgcac atcggcgagt cccacgattt tgtacccggc atcgtgcaaa agcttcgccg
     5521 ccatggagcc gacatttccg aatccttgaa tgatgacgcg cgtgttgacg cgaagcatgc
     5581 gaaatttggc cagcgcacga tcgcaattga aaagaactcc gcgccccgta gcttcgtggc
     5641 ggccgcgcga tcctcccagg tcgactggct tacccgtaac cacggcagtc tcggtatgcc
     5701 gcacgtgcat ggaatacgtg tccatgatcc acgccatggt ttgttcgttg gtgcccagat
     5761 ccggagcagg cacatcgcgc tcaggaccga accattcgat caattcagcc gtgtagcgtc
     5821 gcgtgattcg ttcgagctct ccaactgata atttggtggg atcgcaaatc acgccgccct
     5881 tggccccgcc aaatggaata ttcacgaccg cgcatttcca ggtcatccaa ctggcgagcg
     5941 cgcgcacttc gtccaacgtg acgttgggag cgaatcgaac gccgcctttg cccggtccgc
     6001 ggacaatgga atgatgcacg cggtaaccaa taaatacttc gaggtggccg ttatcgcgaa
     6061 tgacgggaat gtaaacggtg atttctttgt tgggatattt caggaagcgg taaacacctt
     6121 cttcaagacc cagttttcga gcggctgctt caaagcgcgc gtcagcggac tcataaacat
     6181 taaactcatc gcgcccaaca agcgtggcca tggaaaagcg ctcctcacga accctgtcga
     6241 tgccatttac atatcgttcg gcaatcccac gcaaaaaata tttgctgctt caccgatctt
     6301 tgggtaaaag ccgcacgcgg tcagtatacg aatcaaaccg cgcatggcgc aagaaaagga
     6361 atgaattttc gcaagaagat taatgcggtt ttgtcgaagg gggctggcgc tcgataaatt
     6421 cctcgatcaa aagttgcgag agccgctgag cgtcgtgccg cacaacgccg tgctcctcga
     6481 gcaaattatc cagcaaacac cgcacgccca tcctttgaag gtccggcaag tcgacgcgca
     6541 cagattccgc gccgcgccgg ctgtatcgcc tggccacttc cagcgagatt ggctgattgc
     6601 tcgcaacgac ccaatcgaag atttttctcc gcgtatgcga ataaatcacg cggatatggt
     6661 cggccagcga aaatccgtcc gtctctcccg gctgtgtcat cagattcgcc acataaattc
     6721 gtattgccgc ggatttttca atcgcttcgg ccacgccgga aaccagcaga ttgggcagaa
     6781 tgctggtgta caatgaacca ggcccgatca aaatcaagtc cgccttgtgg atcgcctcca
     6841 gcacttcggg tagcgggcga acatggcgag gacgcaaatt gaggcgccga atttttttgc
     6901 ggcatttcgc gatacgtgtc tcgcccgaaa caattttccc gtccgccatc accgcttcca
     6961 gggatacatt ttctaaagtc gttgggaaaa ttcgcccgcg tatcgccagc acttctccgc
     7021 tgacacgcac ggcttcggaa aaatcgcctg tcacgtggct gagggcggta agaaaaagat
     7081 ttccgaaact atgcccacgc aaaccgcggc cggcgtggaa acggtactgg aaaagacgtc
     7141 cgagcagggc ttcatcctgt gaaagggcga ccatgcagtt gcgaatatca ccggggggaa
     7201 gcacacgata ttcgcggcgc aagcgcccgg agctgccacc atcatccgtc acggtaacga
     7261 ttgccgcgag gtcgctgatt ggatggcgct ccgattcatg cctgcgacgc gcaatgaaat
     7321 tcttaagtcc gcgcagcagc atcgaaagcc ctgtgccgcc accaattgcg actacgcgca
     7381 cgcccccatg gcccgtgccg cgttgtcgtg tactcttttt cattgggatt gcatcgttat
     7441 gttgcgaagt cgcggcactt caaactcaag cgccggaagc ttcgcgctca gggtagagca
     7501 attcagtgaa acggggatct tcctggagga atgcaaagtc ttcatcattg cgcgcgtgat
     7561 aacgattttc ggggcgcagt tgaatcgcaa tcttcaaatt ttccatcgcg gattcggctt
     7621 ccccggttaa acaatccagc gctgccatgg cataaaaaac atagtcagcc ttcggtgcca
     7681 acttcttcgc ccgcaatagg tgctcgcgcg cttcgtccca acggcccaaa ttcatcatgg
     7741 ccacgccgga ctgataatgt tcttctggag tgcgtacgga aggcgccgcc tgcttagcca
     7801 tgcgctgctc aacaatgcgc aggtgtactc gtgcgcggtc agctaattcc ttgcttgggc
     7861 cagcctcaac cttctcgagc aattcttttg ctttcacaaa tttttgttga tgaaaatgct
     7921 ggacggcttg ttcgtaaatc tttaattgtt gttcgaattg aggttcaacc atctggcgta
     7981 ccgcttccgc gtgcgctgcg attttactaa cttccttgat tttcaatggc ttacggctgc
     8041 ccttaaccga acctgcccga ccagctcgct ttgtcgactt cccagtcttc ggcattttgg
     8101 ggagtctaac agaggtcgta cctatcgtca acgcagcagc ctgtaatcaa gtgtactcgg
     8161 ggggaggacc cgtttaggct ctccccccat tgctgcgatt cctttacttc tttgtagcag
     8221 gagtgctcgg catccccgtt gccggcattg cggcagatgg tgacgaatcg ggaacaagta
     8281 atttcttggc ggaatctgga agcttatcca cattggctac aaagagagat acttgccaca
     8341 tttgattgtc atccaggatt cccgtgaagg tcggcattcc cgtcagtcga ataccattct
     8401 taatcttcca gaagctttcc tgtggaggat catccgtgac gccttttccc ttaaacatta
     8461 aggttgcgtt ggggtacata ttcaaggcaa tcggcgcagg ctgatttggc aatccgtggc
     8521 aaaccgcgca ttgcgtctta taaagtttcg cgccggccac ataattatct tcgctcggct
     8581 gaatcggtgg cgctggaaca ttggccttct cgatatgcgc gtcgagagat ttgttagcca
     8641 tcttcttttc catcatcatc ggcggatccg caacggctgc tggagccatt cctgacgtaa
     8701 agtagtaata gacagaccca gccagtagaa caacgccaac aagcacacca aacacgaaac
     8761 ccttcatata gttcattctc ccctatacgg aattgagtac cagagtatag ttcaattgcg
     8821 ccctttttct ccgttcaaaa ttaaagaaag agctccgtgc agggcaaatc gcccaaagct
     8881 ttcatcgaaa ggctgggtag gcacatagaa tccataggaa atatcacaac tttgctggca
     8941 aaggaacaaa actctaaaac tcagagactt ctttttggct gcgggttgta tcgcccccta
     9001 tgcttgcggc gcaacaaacc ttggtttgaa cattggactc gcttggcgcg tgtgctataa
     9061 aagaggaccc ggtaagtcag tccggacaac cgtgcctttt atcaactttc ctgctcgcga
     9121 aattaattgc aagttggtct actacggtcc aggtctgggc ggtaagacag ccaacctgca
     9181 gtggatctat gaccacacag gttctgcgca aaaaggcaag atgatttcac tggcaacgga
     9241 gacggatcgg acgctttttt tcgattttct tccgattgac ctgggcacgg tgcgcgggtt
     9301 caaaacgcga atccatcttt ataccgttcc cgggcaggtt ttctatgaag ccagccgaaa
     9361 gttaatcttg aagggcgccg atggcgtcgt atttgtggcg gactcacagg aagagcgctt
     9421 ggacgcaaac ctcgaaacgc tcgaaaacct ccgcgagcac ttgaaagaac acaagctcga
     9481 ctttcaaacg attccttacg tcttacagct caataagcgc gatcttccgg gtgtcctttc
     9541 cattgaagaa cttaccaggc aattacaagt gaagggtgag cccataatgg aagcagtggc
     9601 cattaccgga caaggcgttt ttgaaacgct gcgagaagtc gccaagctgg tcctcgccga
     9661 actgaaaaaa ggtacgtagc gacctctctg cgagtcgata tttctttctg gaaatgacta
     9721 aacttcactt ttgagatgct gtcctaaagt acagtagata ttccagtacg cacggttcat
     9781 tttcttcgta caggatttcc gcggcgattt atcattattg atatgcggat ttcattttct
     9841 tcggcgtgtg gaacgatatg aattccatgc tgcccacaat tcccgacaaa caacaacttc
     9901 cgtcgtctga acggacgctg caagaagctc tggaagactt gcagaagctg gaacgccggg
     9961 actggtggct atggagcatg gccgtcgtgg tcatgctgtt gctgacgttt gccgttttct
    10021 cgatgagctt tccgggcatt attaaggttg atgatcctct ctttgaactc agcttgagcc
    10081 gcgaggtacg tggccttgtc gggctagttc tcatcttcaa tgcctatacc atctatcagc
    10141 agattatggt caagcgattg cggcgggatt attcgagcaa gatcggcgaa atgcgcactc
    10201 tgcaagttcg tgcggaagaa ttggaacgat tggcgcaagt ggatcccctg acagggctgg
    10261 tcaatcggcg tgtcgcggaa gagcatcttg catcggaagc ctcccgatgc aagcgtaacg
    10321 gacatccact taccgtggtt tgttttgatc tcaataactt caagcaaata aatgacacgt
    10381 atggacatct tgccggggat ctggtcctca aacattttgg caggcgcctg caatctgccg
    10441 ttcgaaaatc ggatactgcc gcgcgcatgg gaggagacga gtttcttatc gtgcttcccg
    10501 attgcacaac aagccaggtt caaatcctca tcgccagatt gcgaccgatg gatatcgaat
    10561 ggggcggcgt caaaattccc atttgttttt cggcggggtg ggtgggttac gaaaaaagcg
    10621 agacaccaga acaactcctt gagcgcgccg atcgaatttt atatgcggaa aagcgctccg
    10681 gaaaaacgct tgaaatggaa gcgaatgcaa atgcgcggcg cgagactcag gctaaagcag
    10741 aattatccgc cgctactgtt tcatgatgaa ctttctaagc tctcgcgtat caagtgggtt
    10801 taaacaaagt ttcataaaag agttttccgg cccgactcaa attggttaca aacatgcacc
    10861 cgaatcaaag gtatgattgg atttcgcgga tcatcagaat tttctgaggc tataaaatga
    10921 atgaaataac cacaacccga agacgaagcc agcgcctatt tcttcgcgtt cctgtaatgg
    10981 tcgagggaag tgaaccgaat aaaccccctt ttcgggaaga aacctacacg gtcgtactga
    11041 atgctcacgg cactcttctg gagatgcggg cggcaattga gaccgggcga accgttactt
    11101 tgaaaaacct tagaacaaac gaacaaatag agtgcacggt taaattggtt actccgtctg
    11161 acggcgggaa attcaatgtt gcacttgaat ttagaaaacc caatgcaagc ttctggcaaa
    11221 taagctttcc gccggaagat tgggcccccg caagataaag accccggaag ccccgctggc
    11281 aaacaaggat cgggctcggt ttgttatttt ccccgctggc ttggccagga ccggtaccat
    11341 cttccgatcg ttgtatttgg gagaatcctt ccgcagcctt cctaaactaa aaaagggagg
    11401 caataacctg aacggttacc gcctcccggg gtggggtgtt gctggataaa cttggcaaat
    11461 cgaaatcaat aataggtttt aaatacgcgc ttcacttcca aagcctaagt tacccttaaa
    11521 atctatttta gggaatagca tacggagtat gcggtgtcaa cagcattaat attaaaaata
    11581 tgcctgtttt taatgcgcct aactcagcca ctggacgaaa cggctggaac cgaatccaaa
    11641 acttcacgaa cccttcggca aaggacagac gggctgtaag gctttctgag aattgccacg
    11701 ccatgttcta caaggtcggc caatacggcc gtttcgttgc tatatcccgt tgcgaaaaca
    11761 actgaaatct ggggatttag ctcctttatc acggcaaaaa cttcgggccc gctacgacgg
    11821 ggcatgataa cgtcaagcaa aaccagggaa attttgtcgc ggtgcattgt aaaaatttgg
    11881 atagcttcct ctccgtcatg cgccaacagc acttgatacc ctttggctat caaaactgaa
    11941 taagccattt cgcaaatgcc atcatgatcg tcagcgagca aaatcgtttc gttcccaccg
    12001 cggaccggta aatcatcaaa gacgaggggt cgaaattcag tcttggccgg cacttcattt
    12061 atagggagaa gaattcggaa tgtgctgccc ctgccgggtt cgctctctac ctggatgaat
    12121 ccattgtgct gcttgacgat gccatacacc gtagctaatc ccaatcccgt gcccttaccc
    12181 atgaccttgg tagtaaaaaa aggttcaaaa attctctctc gcgtggcttg gtccattccc
    12241 atgccggtgt cgctcacttg caaagctgcg aagcgtccgg gttgaaggcc cactacgttg
    12301 cgcgcgtctt cctcggaaaa attggcgtta tgggtaacga tcgtgattgt cccgcctttg
    12361 ggcatggcat cacgcgaatt aatgcacaaa ttgatgagca tttgttctat ctgtgtgggg
    12421 tcggcccgga caggcagcag atcgtccgcc agattgcttt taatctcgat atctttttca
    12481 atcaatttgt ccaataggct cattacgtca cgcacggttt gattgaggct cagatttata
    12541 ggctcaagaa tctgcctgcg cgcgaaggcc agtagttgct ggacgagggc tgtcacccgg
    12601 tcgcattgaa tgtgaatttt tttgaaataa tttctcagca ctgcattgcc ggcagtggat
    12661 tgctcttcgc ccatttccgc ccaacctaga atggcgccga tcacgttatt aaaatcgtga
    12721 gcaatccctc cggcaagctg gccgaacgct tcgaattttt gcgcttgttg caactgccgt
    12781 tccagagctc gacgttcggt gacgtccctc gagaccgtgg ccacaaagcg aggctcccct
    12841 gttttttggt cctttacctg aaatcccaca aaatccattg gcaaagcccg tcctgtttgg
    12901 agatggcgga attgcagttc tccctcccaa tggcctatcg tacgaatcgc cggaagaatg
    12961 gtgtcacaca ccagcggaag gtcggccgga attacataat ccagcgagtg cgtccctttg
    13021 atcgagtgcc cactgggaat tccaagcatg gcgcggcccg cggggttcac atattccacg
    13081 ttgtcctgaa gatcggcgac cgcaatgaaa tcccggctgt actcgaccag cgtaaaaaac
    13141 ttttgtttct cctcctctgc ccgctttcgt tccgtaatgt ccagggcaac aacaagtccg
    13201 gcaaccatgc cgcagtaatc catcttttgg agaaacattt ccacgtcgat tagcgaagca
    13261 tccttcttgc ggtggcgcca ttctccggaa agccgctcgt gcaatttggt atctcgaatc
    13321 gcacccaaca atttggaaat ttcttcagct ggatgcaatg ctccggtatt catctgtaga
    13381 aattcagatc gagtaaatcc atagtgtcgc agcgccgcgt cgttcacttg aagaaattgc
    13441 aatgtttcca tttcaaaaac ccacatgggc agcggattct tcgcaaacag aaaaagcgaa
    13501 ttgccttcgc tttggcgcag agcctgaatc atcaactggc gggcatcgcg caaggctctt
    13561 tcttcgagtg ctcgtgctac gaccacgggg agcctggcca agtggtcttt caacacataa
    13621 tccgtaattc cctgcttaat gcattcgact gccacttctt cgcccagcgt ttccgtaacc
    13681 aaaatgaatg gaacgtcacg cccagcgcga cgcatcgctg aaaatgcgtc taagccggtc
    13741 cagccaggca gctggtaatc ggagagaaca acatcaaatg gaaatcgtga aatatgatgc
    13801 aggaattctg ttcgagtgcc aatgatctgt ggtttgcata aaaatccggc acgttccaac
    13861 tcatcaatat ttcttttagc ttccggggac gaattctcaa tcagcagcgg atgcaaaggc
    13921 gcggacatat tccacaatct tgtcaaagca gcattggttg tcgcctgact catgtgaacc
    13981 gctcttcgtc agtcattgtg taatgccgta gcggttcccg cggatctgcg ggacgcctgt
    14041 gcagctttat tccactagaa cccgaacacg gaattgcaga agaggcgcaa cgcggagccg
    14101 aactcgtagc ccgagcgccc gtaatccatt catggatata cacgtttgcc ataaggcaat
    14161 tcgcaaaatt caggaactgc atatgctgcc cgtcctttag cttagaaatg gaatgcggag
    14221 cgcaatgcaa tttcaaatgc attacgccgc gaacacttca caagctgtct atcggcagga
    14281 tttggattgt gcttgaggcg aagtcgatgt tccgctatgg gccatccgag gaaagttatg
    14341 ccaccgttga ttgtttggcg atttgagact cgttattacg agcccgttta tttcgctttt
    14401 cc
//
LOCUS       DASZMB010000177         5761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2389330, whole genome shotgun sequence.
ACCESSION   DASZMB010000177 DASZMB010000000
VERSION     DASZMB010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2389330"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /gene="tatC"
                     /locus_tag="VG272_08040"
     CDS             complement(<1..531)
                     /gene="tatC"
                     /locus_tag="VG272_08040"
                     /inference="COORDINATES: protein motif:HMM:NF013097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HWF13669.1"
                     /translation="MTTPQSELSQVSPEQEPGGKMSFFEHLVDLRKRLINSAMAIAIG
                     AFVGVSVSQKVIGFISRPMQEALRKQHLDDKLIYTSPAGLINIIITFGIYIGVVLAAP
                     YVLYQVWLFIAPGLYKHERKAVSVFIGSSVFLFLAGISFGYFIMLPYMLNFLIGFQGP
                     FRPLISINEYFDFILIV"
     gene            complement(512..928)
                     /locus_tag="VG272_08045"
     CDS             complement(512..928)
                     /locus_tag="VG272_08045"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HWF13670.1"
                     /translation="MLSLPHLAVLFVLALVIFGPQKLPELARMLGKATAEFRKMTNDF
                     RYALEDEVRELDRQTRIREEEKAAAAQVPPTPPVPPTPPEGAVARESGNIDAPPMEIV
                     SSDEGYPPPTEFKSSEPHADTVSVPHEKSPDDNSAV"
     gene            1155..2792
                     /locus_tag="VG272_08050"
     CDS             1155..2792
                     /locus_tag="VG272_08050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HWF13671.1"
                     /translation="MPQESEGEMQGERRGALLVIGVLFVSAVLGGIYGPSVRATSTDV
                     NNLQDSVKSFTHVLSVVQKDYAIPVDTDKAIYSGAIPGMLRVLDPHSSFFDPRAYANL
                     REDQRGRYYGVGMTIVPRDNFTYVLSPMPNSPAYRAGIRPGDVITAVDTKSTEGLTSS
                     EVADMLKGPKGTVVHITMKRVGYNEPINFTVTRDEIPKHSVDLDFDVKPGIGYIKLSG
                     FNETTDSELSAALKDLDATKLDGLILDLRGNPGGLLNEAVAVSDMFLDKNELIVSHHG
                     RASAERRYYAVRGNRGVTVPLVVIVNGGSASASEIVTGAIQDHDRGLVVGEQSFGKGL
                     VQTVSGLSENTGLALTTARYYTPSGRLIQRDYKDISLYDYLYNHKNPAPTEVKLTDSG
                     RQVTGGGGITPDVVVQPMKYNPFQESILRHEVFFAYQGGVGAFTTYFLGTRPEITKDF
                     VANDSVMKTFRAYLDKEKIPFTEEDIAQNLPWIKHEIRKEAFISVFGLTEGAKVDLED
                     DVQLSKAIETLPQARALYENARKIVAQRNAGSGPQQQ"
     gene            2953..3026
                     /locus_tag="VG272_08055"
     tRNA            2953..3026
                     /locus_tag="VG272_08055"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2985..2987,aa:Ala,seq:cgc)
     gene            3267..3464
                     /locus_tag="VG272_08060"
     CDS             3267..3464
                     /locus_tag="VG272_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13672.1"
                     /translation="MAGYNVYREWQFSGPIRLTPQIVQGTQFIDKTAIAGHVYSYYVT
                     SVDSGGLESKPSESISVNVPK"
     gene            3631..4506
                     /locus_tag="VG272_08065"
     CDS             3631..4506
                     /locus_tag="VG272_08065"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HWF13673.1"
                     /translation="MPVRLIALDIDGTLLDEHSHLPAANRDAVAEASVRGIEIALVTG
                     RRYDFAIPIAREFPCPLTMIVNNGALVKSSSGETQLVKLLPVKVAAQVLDETANYREM
                     ASVCFNRPRENQVIYEHIDYEDPLRGAYFVRNREYLGEMIPLRDCLTEDPIQVMFTGY
                     ANQAREVETFLSCLPMASEYSVAMTVYEKRDFGLVDVIARGCSKGSTLAEWVRRRGFE
                     HEDVLAIGDNLNDMEMLQYAGLPVVMGNSVPELKTMGWHETLTNDQCGVAAAIERFAL
                     AGNAVLETPDWKCAK"
     gene            4503..5165
                     /locus_tag="VG272_08070"
     CDS             4503..5165
                     /locus_tag="VG272_08070"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HWF13674.1"
                     /translation="MKRIAQRGGWLAGAMVALAAMAPCARAEYMVLQSGQRIHVTGYE
                     RVGDTLRLTVPGGTMEIPADSIVSVEPEDTFLPAKIQLLDVPYANFISESARAHGVAP
                     ELVASVIAVESNFNPNAVSWRYARGLMQLMPQTAARFGVTKIFDPQQNIDAGTRYLKE
                     LLVRYNGDLSLTLAAYNAGPDRVEQYRAVPPYRETRDYVRRVTEKFHTSYHAASQIVD
                     LR"
     gene            complement(5396..>5761)
                     /locus_tag="VG272_08075"
     CDS             complement(5396..>5761)
                     /locus_tag="VG272_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13675.1"
                     /translation="QVPNATSENPLESQPQPLMGAVLGGNIIGVGSKIKRPSLKVYLG
                     GDTYQEWEFIANTTGQQVVIPGQAPVNPNGIQGGAPGTNGTQPNSTDPNAPGGQQPPQ
                     TPQTPPINIPPPTSPAPSQ"
BASE COUNT         1318 a         1571 c         1562 g         1310 t
ORIGIN      
        1 cacaattaat atgaaatcga aatactcgtt gatgctgatc agcggccgga acggtccctg
       61 aaacccgatc agaaaattca gcatgtacgg aagcattatg aaatagccga aggaaattcc
      121 cgccagaaaa aggaagaccg aagaaccgat gaagacggaa accgctttgc gctcgtgctt
      181 gtaaagtccc ggcgcgatga acagccaaac ctgatacagc acatatggcg cggcgagcac
      241 gacgccaatg taaattccaa acgtgatgat gatgttgatc aggccggccg ggctcgtgta
      301 gatcagctta tcgtccaggt gctgcttccg cagcgcctcc tgcatgggac gggagataaa
      361 tccgatcact ttttgcgaga cgctgacacc gacgaacgcg ccgatggcaa tggccatcgc
      421 cgagttgatg agcctcttgc gcagatccac aaggtgctca aaaaacgaca ttttgccgcc
      481 tggttcttgc tcaggcgaaa cttgggaaag ctcagactgc ggagttgtca tcaggagatt
      541 tctcgtgtgg cacgcttacc gtgtccgcat gcggctcgga cgatttaaac tctgttggcg
      601 gcggatagcc ttcatcactc gatactattt ccattggagg ggcgtcgata ttacctgatt
      661 cgcgcgccac agcgccctca ggaggagtgg gcggcacggg cggtgttggt gggacttgcg
      721 ccgcggcagc cttttcttct tcacgaattc gcgtctggcg atcgagttcg cgcacctcat
      781 cttccaacgc ataacgaaaa tcgttggtca ttttacgaaa ttcggctgtt gccttaccca
      841 acatgcgggc aagttcgggt aacttctgag ggccgaaaat aaccaaggcc aagacgaaaa
      901 gtacggccag gtgtggcaaa ctcaacatgt tcggtacact ttgccttttt cgagcgattg
      961 cagatgcgcc agcggccagg agaagtccaa cgaactcagc cgattagaac tctgacgaaa
     1021 catcctgctt cccagccaga atgactttca ttccgtttct tcctggtgtc tacgtggaat
     1081 catagtggac aggggcaggg gtgtcaattc agcccctatg ctatactatt tggcaatgtg
     1141 cttggccacg tgccatgccg caggaaagcg agggcgaaat gcagggtgag agacgagggg
     1201 cgctgctcgt cattggcgta ttgttcgtat ctgcggtcct gggtggtatt tatggtccgt
     1261 cggtacgcgc tacttccact gacgtaaata acctgcaaga ctcagttaag tcctttacgc
     1321 atgtcctgtc cgtcgttcaa aaagattacg caattcctgt cgatacggat aaagccattt
     1381 attccggagc tatcccggga atgctgcgcg tactcgaccc gcactccagc ttttttgatc
     1441 cgcgcgccta tgccaacctc cgcgaagatc agcgcggcag gtactacggc gttggcatga
     1501 ccatcgtgcc gcgcgacaac tttacgtatg tgctttcgcc gatgcccaat tcccccgctt
     1561 accgcgccgg gattcgtccg ggcgacgtca ttaccgccgt cgacacgaaa tcaacggaag
     1621 gcctgaccag ttcggaagtt gcggacatgt tgaaaggccc caaaggcacc gtcgttcaca
     1681 ttacgatgaa gcgcgttggt tacaacgaac ctatcaactt taccgtgacg cgcgatgaaa
     1741 ttccgaagca ctccgtcgat ctggatttcg acgtaaagcc cggcataggc tacatcaagc
     1801 tttccggatt caatgaaacg acggattcgg aactctccgc cgcgctgaaa gatctggatg
     1861 ccacgaagct tgacgggctc attctggatt tgcgcgggaa tcccggcggc ttgctgaacg
     1921 aagccgttgc cgtttctgac atgttcctgg acaaaaatga attgattgtg tcgcaccatg
     1981 gacgcgcttc cgcggaacgc cgctattacg cggtacgcgg caatcgcggc gtcaccgttc
     2041 ctctggtcgt cattgtgaat ggtggttcgg cttccgcatc ggaaatcgtg acgggagcga
     2101 ttcaggacca cgatcgcggc ttggttgtcg gagagcaaag tttcggcaag ggcttggtgc
     2161 aaaccgtttc cggcctgagc gaaaacaccg gactggcgtt gaccaccgcg cgttattaca
     2221 cgccaagcgg ccgcctcatc cagcgcgatt acaaagatat ttcgctctac gattatctct
     2281 acaaccacaa gaacccggct cccacggaag tgaagctgac agatagtgga cgccaggtca
     2341 ccggtggcgg cggtattacg ccggacgtag tcgttcagcc gatgaaatac aatcccttcc
     2401 aggaatcgat cttgcggcac gaagttttct tcgcgtatca gggcggcgtc ggtgccttta
     2461 ccacttactt cctcgggaca cggccggaaa tcacgaaaga tttcgtagcg aacgatagcg
     2521 taatgaaaac gttccgcgcg tatctcgaca aggaaaaaat tccttttacc gaagaggaca
     2581 tcgcgcaaaa cctgccgtgg atcaagcacg agatccgcaa agaggccttt atctcggtgt
     2641 tcggtttgac ggaaggcgcg aaggttgacc tcgaggacga cgtacagctg tcgaaggcca
     2701 tcgaaacctt gccgcaagct cgcgcgttgt atgagaacgc ccgcaagatc gtcgcgcaac
     2761 gtaatgcggg aagcgggccg caacaacagt aaccgccgtt ccatcgaaac ttattgcgta
     2821 agttcggcga agcatgtttg gattcctcgc cgcaccctgc taaaatctca cgggttcaga
     2881 gtgcggctct gcttggatca gccgtgcaat aattttcaaa aacggatttc atttgtatac
     2941 acagcacgtt tggggggctt agttcagcgg gagaatagtt cgttcgcaac gaacagacac
     3001 gggttcgatt cccgtagcct ccaccaaaat tttattgctg ttgttaatcg tacaaccgaa
     3061 atacgcaaca tagttgtcct tagctctttt tgatgaaaag ctccctccat tcttcgagga
     3121 ctctccgctc aatttttgta ctaactttcc tcacgttgtc gctcttgacg ggttgcggca
     3181 gggcgcggag atttctttcg cgctttcatc atgaaaggct gaattaccac tctgtaacca
     3241 tcagttggac tcccgcgaaa cccaacgtgg cgggatacaa cgtgtaccgg gaatggcaat
     3301 tcagcgggcc gattcgtttg acccctcaaa tcgtgcaagg tactcaattt atcgacaaga
     3361 ctgcaatagc gggccatgtc tactcgtatt acgtgacctc tgtcgattcc ggtggtttgg
     3421 agagcaagcc ttccgaaagt atttccgtga acgtcccgaa gtaaccaaat caaccagcgg
     3481 cggactttgc cttatgtggc ataggcttca gcctgtggcg ttttgacctt gcaagggctc
     3541 acccccacag gctgaagcct atgccaccga accgcacacc tgtacttcat gcgggtggct
     3601 acacggcacg caacaaggta agatcacgag atgcctgtac gcctgattgc gcttgatatc
     3661 gatgggactc tgctcgatga gcactcccac ctacccgcag ccaatcggga cgcggtggcc
     3721 gaggcttccg tccgcggaat cgaaatcgcg cttgtcacgg gccgccgcta cgatttcgcg
     3781 attcccattg cccgcgagtt tccttgtccg ctgaccatga ttgtaaataa cggcgcgcta
     3841 gtgaaatcga gcagcggaga gacgcaactc gtcaagctgc tgcctgtcaa ggtcgcggcg
     3901 caagtgctgg acgagactgc caattaccgg gaaatggctt ccgtctgctt caaccggccg
     3961 cgcgaaaatc aggtgatcta cgaacacatt gattatgaag atccgctgcg cggcgcctac
     4021 tttgtccgca accgtgaata cctcggagag atgattcccc tgcgcgattg cctcacggaa
     4081 gatccgattc aagtgatgtt cacgggctat gcgaatcagg cgcgcgaagt cgaaacattc
     4141 ctttcttgtt tgccaatggc ttcggaatat tctgtggcca tgacggtcta cgaaaaacgc
     4201 gactttggtt tggtggacgt gatcgcacgc ggatgctcca aaggctcgac gctcgccgaa
     4261 tgggtgcggc ggcgtggctt tgaacacgag gatgtattgg ccatcggaga caacctgaac
     4321 gacatggaaa tgctccaata tgccgggctt ccagtggtca tgggaaacag cgtgccagag
     4381 ttgaaaacaa tgggatggca tgaaacactg acgaacgatc agtgcggagt tgccgccgcc
     4441 atcgagcgat tcgcgttggc gggaaatgca gtgttggaga cgccggattg gaagtgcgcg
     4501 aaatgaagcg gattgcacag cgcggcggtt ggcttgccgg cgcgatggtt gcgcttgcgg
     4561 cgatggctcc gtgcgctcgc gccgaataca tggtgttgca atccgggcag cgcattcacg
     4621 taacgggcta cgaacgcgtg ggagacacgc tgcggctcac tgtccctggc gggacaatgg
     4681 aaattcccgc ggactcaatt gtgagcgtgg aaccggaaga tacttttctg cccgccaaaa
     4741 tacagttgct cgatgttccc tacgccaatt ttatttccga atccgcgcgc gcccacggcg
     4801 ttgcgcccga actcgtcgcc agcgtgattg ccgtcgaatc caatttcaat cccaacgccg
     4861 tgtcctggcg ctatgctcgc ggcctgatgc aattaatgcc gcaaaccgcc gcccgctttg
     4921 gcgtaacgaa aattttcgat ccgcaacaaa acatcgatgc cggcacgcgc tacttgaaag
     4981 aattgctggt gcgttacaac ggcgaccttt ctttgacgct tgccgcctat aacgctggcc
     5041 ctgaccgcgt cgaacagtac cgcgccgttc ctccctaccg cgaaacgcga gactacgttc
     5101 gtcgcgtcac ggaaaaattc cacacctcat atcatgccgc gagtcagatc gtggatttgc
     5161 gataattttt acgtgggaca ggctttacag tttgcggaaa aagtcgttta tcctgtcatt
     5221 ccgagcgaag cgaggaatct ctcttcgatt taagcataag aaaataaaga aggagggatt
     5281 cctcgcttcg ctcggaatga cagaatgttc agttcttccg cagtctgttc agcctgtccg
     5341 gtttaaattt cgttcggact agcaaagttc agaagaagac aggttgaagc ccgtcctact
     5401 gagagggtgc aggcgaagtc ggcggcggaa tattgatagg cggcgtttgc ggtgtctgcg
     5461 gaggttgctg ccctccggga gcatttggat ccgtactgtt cggttgcgtg ccattcgtgc
     5521 cgggagcacc gccctgaatt ccatttggat tcacaggcgc ctgccccgga atcacaacct
     5581 gttgcccagt cgtgttggcg ataaattccc attcctgata cgtatcgcca cccagataca
     5641 ctttcagcga aggtctctta atcttgctgc caaccccaat aatatttccg cccagcacgg
     5701 cgcccatcaa cggttgcggc tgcgattcca gaggattctc tgacgtcgca ttgggcacct
     5761 g
//
LOCUS       DASZMB010000178         3617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1314756, whole genome shotgun sequence.
ACCESSION   DASZMB010000178 DASZMB010000000
VERSION     DASZMB010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314756"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(389..1747)
                     /locus_tag="VG272_08080"
     CDS             complement(389..1747)
                     /locus_tag="VG272_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWF13676.1"
                     /translation="MLHDVILFLGFLGVIAGLFLAFLSQRYWFARAWKFAGRIHDPIS
                     RKRLRGALIGLVAFVALLALAAATRNMRGVVSHGSWWTAFFGFWLTSSIFSYLFIKII
                     AGAEWMWKRVRRPSAEKPEPIAIAPNLAVAGAHHEASEHIDHSRRYFFQAAGVIAGAV
                     PFVSAAYGFVAERLQFSVREVEIPIANLPPALDGLRITQLSDIHIGSYMPTSQVRRAV
                     GMANELKGDLTVVTGDFVTGKSDPLEECITELSRLRAPLGVWGCNGNHEIYAEAEAKS
                     AQLFQRFGMKLLRLENAELRWQGSAFNLIGVDYQRQRDTEGKHPPMLLGVDRLVRRDV
                     PNILLSHNPNSFRKAAELGIELSLAGHTHGGQVKVEILDHRWSPAQFLTPYVAGLYRR
                     PLLAPANTGDAADSSGPLVAVSDLHCSSAIYVNRGLGTIGAPVRLGVPPEITLITLRR
                     AV"
     gene            complement(2060..>3617)
                     /locus_tag="VG272_08085"
     CDS             complement(2060..>3617)
                     /locus_tag="VG272_08085"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWF13677.1"
                     /translation="INYKGQLGRVTALRAEPANAPLAASRVFVDDQNGTLYIMDTATK
                     KFTPYLKFKDIFPNFASDKGNASGIVSITFDPSYAKNGKFYTIHVENPALSGTATPVN
                     TATPTLNLTGYSTTDTVNPPSGPVHLESVLVEWTDTNIRNATFEGSARELLRIGFDRN
                     HPSDDTIFNPLAKPGSPDYGNLYIGVGDGAQGETPGPTHTIPQQLNTLLGKILRITPD
                     LNLHPNDMLSPNGRYRIPSTGPDPNPFVKVAGARGEVYAYGLRHPHRFDWDTTTKTFL
                     VIDIGLHYWEEIDIVAKGANYGYPEREGNEQLFVESNGKTGSLMTPPVAFPDRDLLKV
                     EGIDEPVIPIYPAAVYSHWEGDSIGSGFVYRGKLMPQMRGKFIFNDMTTGRIFYTDLN
                     EMIATRGQRNHQAPIHELQIMYKDPKDSSQPAAKKRMFDIIAQTYAQKGGTPGPDHVL
                     PGSAQATTGWRDDDHKQPKADKDGVAYGGGRADVRIALGGDGEIYVLSKSDGMIRKMT
                     SVVTPPPTAK"
BASE COUNT          788 a          959 c         1028 g          842 t
ORIGIN      
        1 tgctctgggc aagtgtccca ttcgccgcag ccgtttcctt cagccgcttc tcaacccaag
       61 cgtctcgcac tcccatcgct ttccccctga agagtctccg ctcgcggagc gtctctcttg
      121 tgtaagcccg aattatcgca ccgctccgcg tgcgcagcaa cctgagtcaa cgccttaagg
      181 cgatttcggg agcgcagatt tcgaccaaag gaacagcaat aaaatgtctg tcattccgag
      241 cagagcgagg aatctctctg ttccttttcg cccgtacaaa tcgaagagag attcctcgct
      301 tcgctcggaa cgacaggagg ttggatgttt ttaggggcct agcaaacctt tgggattgaa
      361 accttgcaag gactcaagcc cgcgcaactt aaaccgcgcg acgtagggtg attagggtta
      421 tttctggagg aacgcccagg cgcaccggcg ccccaatcgt gcctaggccg cgattgacgt
      481 agatggcact ggagcaatgc aggtcggaaa ccgcaacgag cggaccgctg gaatccgctg
      541 cgtcccctgt atttgccggg gccaaaagag ggcgccggta aaggcctgcg acgtatggcg
      601 taagaaattg cgcgggactc cagcgatgat ccagaatttc cactttcacc tgtccgccgt
      661 gtgtgtgtcc ggcaagcgaa agctcgatcc ccaattcagc ggctttgcga aaagaatttg
      721 gattatggga aagcaaaatg ttgggaacgt cgcgccggac gaggcggtcc acgccaagaa
      781 gcatgggcgg atgttttcct tctgtgtcac gctggcgctg gtaatccact ccaattaaat
      841 tgaatgcgct tccctgccag cgaagttcgg cattttcgag gcgcagcagc ttcatcccaa
      901 atcgctgaaa aagttgcgcg gattttgctt ccgcttcggc atagatttca tggtttccat
      961 tgcaacccca cacgccaagg ggtgcgcgca atcgcgaaag ctctgtaatg cactcttcga
     1021 gcggatcaga tttcccggtc acaaaatcgc cggtgacgac tgtcaagtcg cccttcaatt
     1081 cattcgccat gccaaccgca cgacgcactt gcgacgtggg catgtaggag ccgatgtgaa
     1141 tatcgctgag ttgcgtgatg cgcaatccgt ccagcgcggg cggaagattg gcgatgggaa
     1201 tttcgacttc gcgcaccgaa aattgcagcc gttccgcgac aaatccatag gcggcgctca
     1261 cgaacggcac ggcgccggcg atgactccgg cagcctggaa gaaatatcgg cgcgagtgat
     1321 cgatgtgttc ggacgcttcg tggtgagcgc ctgcaaccgc gagattgggc gctatcgcaa
     1381 tgggctcggg cttctcagca gaaggccttc gcacccgctt ccacatccac tccgcgcccg
     1441 caattatttt gatgaagaga taggaaaaaa tggagcttgt gagccagaaa ccgaaaaagg
     1501 cggtccacca ggaaccatgg gaaacaacgc cgcgcatatt tcgcgtggca gcggcgagcg
     1561 ccaacagagc gacgaaggcg acaagcccga tcagcgctcc acgtaatctc ttgcgcgaga
     1621 tcgggtcgtg aattcgcccg gcaaatttcc atgcccgcgc aaaccaatag cgctgcgaaa
     1681 gaaatgccag aaaaagtccc gcgatgacgc ccagaaaacc gagaaaaagg ataacgtcat
     1741 gaagcaataa gtatgcgccc acgctaatag gatactattt ttgcggaata gttgcacggg
     1801 agcgtggagg atgtgccgtt aggtaaacct gtttctgatg ctgaaagcgc ttgatttttg
     1861 gagttaattc tccataacag atggtagctg ctggagttaa caagtgcttg ggatttcata
     1921 tttcatgatg atatcttttt gtccggattt tttctttcag tgcgaatcaa aaccgccggc
     1981 aggacgccgc cgccacaaaa acgcgccttc cgtattcgcg gaaggcgcgt ttttttgttt
     2041 actcgtttgt gtcactcaat tattttgcgg tcggtggggg agtgactact gacgtcatct
     2101 ttcggatcat gccgtcggac ttgctcagga cgtagatttc gccgtcgccg ccgagggcaa
     2161 tgcgaacatc tgcgcggccg ccaccgtagg cgactccgtc cttgtcggct ttgggttgct
     2221 tgtggtcatc gtcgcgccat ccggttgtcg cctgtgcgct gccgggtagc acatgatctg
     2281 gaccaggcgt tcctcccttt tgcgcatagg tctgagcaat gatgtcaaac atgcgcttct
     2341 tcgcggcggg ctggctcgag tccttaggat ctttgtacat gatctgcagc tcatgaatcg
     2401 gcgcctgatg attgcgctgg ccccgtgttg cgatcatttc gttcagatcg gtgtaaaaaa
     2461 ttctaccggt ggtcatgtcg ttaaaaataa attttccgcg catctgcggc atcaacttgc
     2521 cgcggtacac gaaaccgctg cctatggaat cgccttccca gtggctatac acggcggctg
     2581 ggtaaatcgg aatcaccggc tcgtcgatac cttctacttt caggaggtcg cgatcgggaa
     2641 acgcaacggg cggggtcatc aagctgcccg tcttgccatt actttcgaca aataattgtt
     2701 cgttgccctc gcgctccggg tagccatagt tggcgccctt ggcgacgatg tcaatttctt
     2761 cccaatagtg cagaccgata tcaatcacca ggaaagtctt ggttgtcgtg tcccaatcga
     2821 aacggtgcgg atggcgcaaa ccgtatgcgt aaacttcgcc gcgtgcacct gccactttca
     2881 cgaatggatt cgggtccggt ccggtggacg gaatgcggta gcggccgttg ggactgagca
     2941 tgtcatttgg atgcagattc aggtcgggag tgatccgcag gattttgccg agcaaggtgt
     3001 tgagctgttg cggaatcgtg tgcgtggggc cgggcgtttc gccctgcgcg ccatcgccta
     3061 cgccgatgta gaggtttccg tagtcggggc tgcccggttt ggccagagga ttgaagatcg
     3121 tgtcatcgct cggatgattg cgatcgaatc caatgcgcag gagttcgcgc gcggaaccct
     3181 caaacgtggc gttgcgaatg ttcgtgtcgg tccattcgac gagcacggat tccagatgga
     3241 ccgggcccga aggcggattc acggtgtcgg tggtggaata accagtcaaa ttcaaagtcg
     3301 gagtcgcggt atttacaggc gtcgcggttc ccgacaacgc aggattttcc acgtggatgg
     3361 tataaaactt tccgttcttt gcgtaactcg gatcgaaggt aatggaaacg attccggaag
     3421 catttccttt gtccgaagca aaattcggaa aaatatcctt gaattttaaa tagggtgtaa
     3481 attttttggt cgcagtatcc atgatgtaca gcgtgccgtt ttgatcgtcc acaaaaacgc
     3541 gtgaggcggc aagcggcgcg ttggctggct cggcgcgcaa tgccgtgacg cgtcctagtt
     3601 gccctttgta attgatc
//
LOCUS       DASZMB010000179         8564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2105155, whole genome shotgun sequence.
ACCESSION   DASZMB010000179 DASZMB010000000
VERSION     DASZMB010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8564
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2105155"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /gene="hpxZ"
                     /locus_tag="VG272_08090"
     CDS             complement(<1..370)
                     /gene="hpxZ"
                     /locus_tag="VG272_08090"
                     /inference="COORDINATES: protein motif:HMM:NF022968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalurate catabolism protein HpxZ"
                     /protein_id="HWF13678.1"
                     /translation="MQINDPKIVAELQELYPKYEAALMANDAEMLTQMFWASTDAMRF
                     GVTENLYGIEEIEAFRKSRPSINLARAVRRLDIVTFGRDFGSVTLEFERTTNGKKISG
                     RQSQVWVRLPEGWRIVSAHVS"
     gene            complement(462..1406)
                     /locus_tag="VG272_08095"
     CDS             complement(462..1406)
                     /locus_tag="VG272_08095"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HWF13679.1"
                     /translation="MAELSVNAEPTHSLWNRALKPRLHVASGDILHMECVDSSGAQVH
                     PSMTLAEYLKIDRAKIHALTGPIFVEGAEPGDVLQVDILEVAHKGWGWTSVVPGLGFL
                     KQRFAEPFFHIWKFEGDVSKSLPPAVVPLNPFCGVMGVAPAEDGEFKTRPPGIFGGNM
                     DVRELCTGSTLYLPVLNRGALFSAGDAHGAQGDGEVCINGMECPADVTLRFQVHKQRS
                     LPGAIVESTAGRKLESDAAGGEWIVVESSNDAVGAARAATSRMIDLLADRWGLSAVHA
                     YLLCSVAMNLRFSQVVNEPIFTVSAAISKNILPARKLF"
     gene            complement(1415..1876)
                     /locus_tag="VG272_08100"
     CDS             complement(1415..1876)
                     /locus_tag="VG272_08100"
                     /inference="COORDINATES: protein motif:HMM:NF025939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWF13680.1"
                     /translation="MVTGRIEGRLNELSIVLPALPQPGGNYLPARTVGQMVYLAGVIS
                     IHAGDVISGTAGGDRTIEEGYSAARACALIQLAVLASHLGSLDKVKNIVTVNGYVNAV
                     AGFRDSPKVINGASDLFVEIFGDAGRHARTAIGVPALPRNALVELQMTVEV"
     gene            2122..2511
                     /locus_tag="VG272_08105"
     CDS             2122..2511
                     /locus_tag="VG272_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13681.1"
                     /translation="MKSLTWLLLLLVAGPVWAKPKVDVRIRVNDGVGRDRKDSLSRTG
                     SSYDGALTTGQDYFLNVTVVSDNAEVVAKNSGQWCIKGETLLGAIDYKATLEGNYLEI
                     EVPQKNGKIKKITFGIFDRKWHKLSDL"
     gene            2585..3460
                     /locus_tag="VG272_08110"
     CDS             2585..3460
                     /locus_tag="VG272_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13682.1"
                     /translation="MHFSLRYLVAFLAFFLVTTTAQSFPTLHEKRQQASDLEVSGALA
                     GLPPDSVRYITRDELLAMPQVSFIASGDTNFTGVTRIRGVKIQDLARALGASPSSDMV
                     VAICDDEYRANYPRSYLMAHHPVLVLEVNGKAPAGWPKDSQEHKYDMGPYMISNPKFV
                     PSFKILSHTDEPQIPWGVVRIEFRDEKTVFDAIAPRGPDAQDREVRNGFRIAKQNCFR
                     CHNFGREGGQKSGLSWEVLASLADESPKEFAAYIHAPLSKNPKAQMSGFPEYDDATLA
                     ALTSYFRSFSTQAKP"
     gene            3457..3954
                     /locus_tag="VG272_08115"
     CDS             3457..3954
                     /locus_tag="VG272_08115"
                     /inference="COORDINATES: protein motif:HMM:NF021453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2165 domain-containing protein"
                     /protein_id="HWF13683.1"
                     /translation="MILRTIKIVLVFGVAIFYTLVVFNNVTDYNSNYQFVRHVLMMDS
                     TFPGNRGMWRAINTPLLHTAFYVSIITWETITMILCWWGGIRMARTRRESAVVFQQSK
                     RISIAAMALSLLMWLVAFLAVGGEWFLMWQSRMWNGQDAAFRMFTVTGIVLLFVAQPD
                     VADQP"
     gene            complement(3981..4385)
                     /locus_tag="VG272_08120"
     CDS             complement(3981..4385)
                     /locus_tag="VG272_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWF13684.1"
                     /translation="MIIGDRLRDLREQKKLSQGEIEERTGLLRCYISRVENGHTVPSV
                     ETLEKFARALEVPMYQLFYDGNLNSRLPNLTKRKSGNGFHWGESGKDARTLARFRQLL
                     GRADEDDRQLLMHMAKKMAKTNAPRAEVRTSK"
     gene            complement(4562..6826)
                     /locus_tag="VG272_08125"
     CDS             complement(4562..6826)
                     /locus_tag="VG272_08125"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWF13685.1"
                     /translation="METKSISRRDLLKSTGVMIVGFSLFGSAAKVLAQGSGLSVDGMD
                     PTVLDSWLAISKDGTVTVFTGKVELGTGVVTALAQIVAEELDVAFNKVYMESGDTDTA
                     VDQGVTAAARTVERGGPQLRQASAAARQELLKLASARLDSPVDKLTVTDGVVSVIGNP
                     AKKIAYGDLLGGKKFNIKIVASGIGWDMKVAPEIPAKNPKDYKIVGQSIPRVDLPGKF
                     TGEFVYSQDVSVPGMLHGRTVRPATSLSAPLSVDESSIKNIPGVVKVVREGNFVGVVA
                     QTEWGAIQAARTLKVTWAEPTLKMPSGADEVFDYLKNTKSFKDNVVANKGNVDAALAQ
                     AHKKYEATYYWPFQLHGMIGPPCAVADVQGDKATIWTGTQGPFRTRDAIALLLKVPPK
                     NVHLLYREGSGSYGRLESDDVAEDAALMSRAVGRPVRVQWMREDEHAWDPKGPAQYST
                     VRAGVDVQGNVIAWDYMDRSLPWTESEANPLLASSQVGMKPTGPGFLNGAGGGGQIYT
                     IENQKVLAADVPWVQQGQWPLRTSNLRAPGDLARVFASESTIDEIASDLHVDPVQFRL
                     RYLTSDKRISEILVATAKQAGWADRPAPGLASSGNKATGRGVAVANRANTMTAAVAEV
                     EVDKTSGEVSVKKITLGHDCGLIVNPNGLKNQIEGNILQGVSRALLEEVQFDSSGQKN
                     LDWNSYPVIRFEQVPEVDVILTNRPEMQPLGGGEPSIVPVTAAIANAIFDATGARLRR
                     VPFTPERVLSAMKV"
     gene            complement(6843..7328)
                     /locus_tag="VG272_08130"
     CDS             complement(6843..7328)
                     /locus_tag="VG272_08130"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWF13686.1"
                     /translation="MARISLKVNGKSRAVEADPSTPLLYVLRDNLELHGPRFGCGLGQ
                     CGACTVLMDGNSVRSCMIPASSAQNKNIVTLEGLGSVDHPHAIQAAFVEEQAAQCGFC
                     MNGMVMGAKSLLDKNPHPTVAEIKKSLNSYICRCGTHLRVIRAIQRAANPGANGKLIA
                     S"
     gene            complement(7544..8296)
                     /locus_tag="VG272_08135"
     CDS             complement(7544..8296)
                     /locus_tag="VG272_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13687.1"
                     /translation="MMFSKIKSTTCFAFLCGLFFFPAGRVFAQAPPGPMSPSQPLPPP
                     KTAPPAKKKTEVPPRKTLAGFWKLNMDESDDARNKLDEARRTKAGGGNGPVDPGIPGN
                     GRVGVGFPIPGSGGNGPYGGSGRGMGGGDSETSQRIFEQIRPDYTQNISLTNAEVDSL
                     NDHGDKLVFFTDGRKIQKSKNEALHEIPAHWDGPKLVTDEKGPQNKKMSRTIELSADG
                     RQVYETWRIENGKSGAPITIRYVFDAADEYAR"
BASE COUNT         1925 a         2378 c         2177 g         2084 t
ORIGIN      
        1 ctgatacgtg ggctgagacg attcgccagc cttccggaag gcgcacccaa acctggcttt
       61 gacggccaga aattttcttt ccatttgttg tgcgttcgaa ttcgagtgtg acgctaccga
      121 agtcgcgtcc aaacgtgacg atatccagcc gcctgacggc acgggctaaa tttattgaag
      181 ggcggctctt gcggaacgct tcaatctctt cgatgccgta caaattttcc gtaactccga
      241 aacgcattgc atccgttgac gcccaaaaca tttgagtgag catttccgca tcattggcca
      301 ttagcgcggc ttcatacttg gggtagagct cttgcagctc cgcgacaatt tttggatcat
      361 taatttgcat ttattttgct ttcgttgcac aggcttcaga ccgtgggttt ctgctgattc
      421 aaaatctaaa tccacgggct gaagcctgta ctactttcca gttagaaaag ttttcttgcg
      481 ggcagaatat tctttgagat tgccgccgaa acagtgaata tcggttcgtt gaccacctgg
      541 ctgaaacgca ggttcatcgc cacgctgcac aatagatatg catgcacggc gctcaatccc
      601 cagcgatctg caagcagatc gatcatgcgg gaagtcgccg cgcgcgcagc gcccacggca
      661 tcgtttgacg attcaacgac aatccattca ccgccggctg cgtccgattc cagcttgcgc
      721 cccgcagtcg attccacgat ggctccggga agcgagcgtt gcttatggac ttgaaagcgc
      781 agcgtcacat ccgcggggca ttccattccg ttaatgcaca cttctccatc cccttgggcg
      841 ccgtgggcgt cgccggccga aaaaagcgcg ccgcgattga gcaccggcaa atacaacgtc
      901 gagccggtgc atagctcgcg cacatccatg ttgccgccaa aaattccggg tggacgcgtc
      961 ttaaattcgc cgtcttcggc cggcgcaaca cccatcacgc cgcaaaacgg atttaagggc
     1021 accacggcgg gcgggagcga cttgctcacg tctccctcaa atttccagat gtggaaaaac
     1081 ggctccgcga aacgctgctt gagaaaaccg aggcccggca caacgctggt ccagccccag
     1141 cctttgtgcg ccacttccag aatgtccacc tgaaggacgt cgcccggctc ggctccctcc
     1201 acaaaaatcg gccctgtgag cgcatgaatc ttggcgcgat cgattttcaa atattccgcg
     1261 agcgtcatgg acggatgaac ttgcgcgccg ctggaatcga cgcattccat atgaagtatg
     1321 tctccagacg cgacatgaag acgcggcttc agtgcgcgat tccaaaggga gtgagtgggt
     1381 tccgcgttta cggacaattc agccataggc ctgcttaaac ctccaccgtc atttgcaatt
     1441 cgacgagcgc gttgcgaggc aacgccggaa ctcctatcgc cgttcgtgca tgtcgtccag
     1501 catctccaaa aatctcaaca aaaagatcgg atgcgccgtt gatgactttt ggcgaatccc
     1561 gaaaaccggc aaccgcgttc acatatccat tgaccgtaac aatatttttt accttgtcga
     1621 gagagccaag atgagaggca aggacggcca attgaatcaa cgcgcacgcg cgggcagccg
     1681 agtaaccctc ttcgatcgtt cgatctcctc cggcagttcc gctgatcaca tctccagcgt
     1741 ggatcgaaat gacaccggct aaataaacca tttgcccaac cgtccttgcc ggaaggtaat
     1801 tgcctcctgg ttggggcaat gccggaagaa cgatgcttag ctcatttagc cttccctcga
     1861 ttctcccagt aaccataaat accagagacc tctgtcaaaa ggaatcacgg aagtttagca
     1921 tatacaatca ctcggaggat ttcctggggc tgcactcctc gccaaagatt actccgggac
     1981 gccaggttcc ctgccgctgt tactgtgaca gtaatattat ttctaagaaa taaagagaaa
     2041 gtgtagaatc tcgaacggta attcccaggt attcgtcccc atccatcaag gaaacgacgc
     2101 atgagaaagg agaacgataa aatgaaatca ctgacatggt tactcttgct gttagtggct
     2161 ggacccgtct gggcaaaacc gaaggtcgac gttcggatca gggtgaacga cggtgtcggg
     2221 agggaccgca aagactcgct ctccaggact gggtcttcgt acgacggcgc actaactacc
     2281 ggccaagatt acttcttgaa tgtaacagtt gtgtccgaca atgcggaagt ggtggccaag
     2341 aatagtgggc aatggtgcat taagggcgaa actttactgg gagccatcga ctacaaagca
     2401 acgcttgagg gaaattacct ggagattgaa gttccgcaaa aaaacgggaa aatcaagaag
     2461 ataacctttg ggatttttga ccgaaaatgg cacaaacttt cggaccttta gtcctcgggc
     2521 gactattcgc ctgctcagcc ggatttttcg tttgcgccaa cgcatctcta cgctagaata
     2581 ttccatgcat ttttcgttga gatacctcgt cgctttcctg gcattttttc ttgtcacgac
     2641 gaccgcgcaa tcttttccca ccttgcacga gaagaggcag caagcatccg acctggaagt
     2701 gagcggagcg ctcgcgggac ttccacccga ttcggtcaga tacattacgc gcgacgaatt
     2761 gctggccatg ccgcaagtaa gctttatcgc atccggcgat acaaatttta ccggcgtgac
     2821 ccgaattcgc ggcgtgaaga ttcaggatct agcacgcgcg ctgggcgcgt ctccttcctc
     2881 tgatatggtt gtagctattt gcgacgatga ataccgcgca aattatccgc gttcctacct
     2941 catggctcat catcctgtgc tcgtgctcga agtaaatgga aaggcgcctg ccggatggcc
     3001 aaaagattct caagaacata aatatgatat ggggccttac atgatctcga atccgaaatt
     3061 tgtgcctagc ttcaaaattc tttcgcacac ggatgagccg caaattccat ggggcgtggt
     3121 gcggattgaa tttcgcgatg agaaaactgt tttcgatgcg attgcgccgc gcgggccaga
     3181 cgcgcaggac cgcgaagtgc ggaatgggtt ccgcatcgcc aaacaaaatt gttttcgctg
     3241 ccacaacttt gggcgcgaag gtggacagaa atcgggcttg tcctgggaag tgcttgcgtc
     3301 tctggcggac gaatcgccaa aagaatttgc cgcctatatt cacgcgccgc tgagcaagaa
     3361 tccgaaagcg caaatgtccg gcttccctga atatgacgat gcgacgctgg cagctcttac
     3421 ttcctacttt cgaagttttt caacgcaggc aaaaccatga tcctgcgaac aattaaaatc
     3481 gtattggttt tcggagtggc gattttttat acgctggtcg ttttcaataa cgtcacggat
     3541 tacaattcca attatcaatt cgtacggcac gtattgatga tggattccac gtttcccggc
     3601 aatcgtggca tgtggcgcgc gataaacacg ccgcttttac acacggcttt ctacgtttcg
     3661 attatcacgt gggaaacgat cacgatgatt ctgtgctggt ggggcgggat tcgtatggcg
     3721 cggacgcggc gggaatccgc ggtggttttt cagcagtcca aaagaatttc cattgcggcg
     3781 atggcgctga gtctgctcat gtggctggtt gcgttccttg ccgtgggtgg cgaatggttt
     3841 ttgatgtggc aatcaagaat gtggaatggt caggacgcgg cttttcgtat gttcacggta
     3901 acgggtattg tattgctgtt cgtggcgcaa ccagacgtgg cggatcaacc ctagctgttt
     3961 ggcccaggta ttcggcctgg ttacttggat gtccgaactt ctgcgcgcgg cgcatttgtc
     4021 ttcgccatct ttttcgccat gtgcatcaga agttgccggt catcctcatc cgcgcgaccc
     4081 agcagctgac gaaatcttgc cagcgttcgc gcgtcctttc cgctttctcc ccaatggaag
     4141 ccatttccag attttcgttt cgtaagatta ggaagccttg agttcagatt tccgtcgtaa
     4201 aatagttggt acatcgggac ttccagagcg cgcgcaaatt tttcaagagt ttcaacggac
     4261 gggacagtgt gcccgttttc tacgcgcgaa atatagcaac gaagaaggcc tgttcgttct
     4321 tcaatctcgc cttgagataa ttttttctgc tcccgcagat ctcgcaatcg gtcgccaatg
     4381 atcataatca gggaacctcc aaccggtaat atttacgaat cgtttaatag aaggcgtgac
     4441 tacttagctg ggcagaaaca gcatacatca gaagattaat tcgtgacagc tttttcgccg
     4501 cgcttcacgc gaagtggttg tcacagtaaa tgtcacaatg cgctctggcg aatgttgcgg
     4561 tctaaacttt catcgcgctc aaaacgcgct ctggcgtaaa cggaacacga cgcagccgcg
     4621 cgcccgtagc gtcaaaaatg gcattcgcaa tagccgctgt gacaggaacg atggacggtt
     4681 cgcctccacc taatggctgc atttccggcc gattcgtcag gatgacgtcg acttccggaa
     4741 cctgttcaaa acggataacc ggatagctat tccaatccag attcttttgt ccgctggagt
     4801 cgaattggac ttcttcgagc agcgcacggc tcacgccctg caaaatattt ccttcgatct
     4861 ggtttttcaa cccgttcgga ttcacgatca ggccgcaatc gtggcctaaa gtaattttct
     4921 ttacgctgac ttcgccgctc gttttgtcga cttcaacttc ggcgacggca gctgtcatcg
     4981 tgttcgcacg attcgcaacc gcaacacccc ttcctgtcgc tttgttgccg ctggaagcaa
     5041 gtcccggtgc agggcggtcg gcccatccgg cttgtttggc ggtcgcgaca agaatttcag
     5101 agatgcgttt atcgctcgtg aggtagcgca ggcggaattg aaccggatcg acatgtaaat
     5161 ccgaagcaat ttcatcaatc gtggactcgc tcgcaaacac gcgcgccaaa tctcccggcg
     5221 cacgaagatt gctggttcgc agcggccatt gcccttgctg gacccagggt acgtctgccg
     5281 ctaacacttt ttgattttcg atcgtataga tttgaccgcc gccgcccgct ccgttcagga
     5341 agccgggccc ggtcggtttc atgccgacct ggctcgatgc caagagaggg tttgcttccg
     5401 actcggtcca cgggagactt cgatccatgt aatcccatgc aatcacgttg ccctgtacgt
     5461 ccacgcctgc gcgcacggtc gaatattgcg caggtccttt aggatcccag gcatgttcgt
     5521 cctcgcgcat ccactgcacg cggacaggtc tgcccacagc gcgagacatc aacgccgcgt
     5581 cttcggccac gtcatcgctt tccaatcgac cgtagctgcc tgaaccttcg cggtaaagaa
     5641 ggtgaacatt tttcggcgga actttcagca gcaaggcgat ggcatcgcgc gtgcgaaaag
     5701 gaccttgcgt cccagtccag atcgtagctt tatctccctg tacgtcggca accgcgcagg
     5761 gaggcccgat cattccgtgc aactgaaacg gccagtaata agtagcttca tatttcttgt
     5821 gcgcctgagc caaagcggcg tccacatttc ctttattcgc tacaacgtta tccttgaaac
     5881 tcttcgtatt tttcagataa tcaaaaactt cgtccgcgcc ggaaggcatt tttagcgttg
     5941 gttcggccca cgtaactttc agcgtccgcg cagcctgaat cgcaccccac tccgtctgag
     6001 ccacaactcc cacaaaattt ccttccctta cgactttcac tacgcccgga atatttttga
     6061 tcgagctttc atcgacgctc aacggcgcgg aaagtgacgt tgcaggacgc acggtccgcc
     6121 catgcagcat tcccggcaca ctgacgtcct gcgagtacac aaattctccg gtaaactttc
     6181 ccggcaggtc cacgcgagga atggattgcc caacaatctt gtaatctttg ggattcttgg
     6241 cggggatttc cggcgccacc ttcatgtccc agccaatccc gctggcaacg attttaatat
     6301 tgaatttttt tccgcccagc aagtctccgt acgcaatttt cttcgccgga ttgccaataa
     6361 cgctgaccac gccgtccgtc acggtaagtt tgtctacagg agaatcgagc cgcgcggatg
     6421 ccagcttcaa aagttcttgc cttgccgcgg cagatgcttg ccgcagttgc ggcccgccgc
     6481 gctccacagt tctggcggcg gcagtaactc cctgatcgac ggccgtatcc gtgtcgcccg
     6541 attccatgta aaccttgttg aacgccacat cgagttcctc ggccacaatc tgcgccaagg
     6601 cggttacaac gcctgtgccg agttcgacct tgccggtaaa aaccgtaacc gttccatcct
     6661 tcgaaatggc cagccaggaa tcgagcaccg tgggatccat gccgtccacg gaaagtcctg
     6721 aaccctgtgc gagcactttc gccgcgcttc caaaaaggct gaagcccacg atcatgacgc
     6781 ctgtgctctt aagaagatct ctgcgggaaa tggatttcgt ttccatgatc ggctctcctt
     6841 ttttagctcg caatgagctt gccatttgcg cccggatttg cggcgcgctg aatcgcgcgg
     6901 atgacccgca ggtgagtgcc gcatcggcaa atatagctat tcaatgattt tttaatttcc
     6961 gcgacggtgg gatgaggatt tttgtccagc agcgacttgg ctcccatcac cattccattc
     7021 atgcagaagc cgcactgcgc agcctgttcc tcaacgaacg cagcctgaat cgcgtgagga
     7081 tgatcgacgc tgcccaatcc ctcaagagtg acaatatttt tattttgcgc cgaggaggca
     7141 ggaatcatgc acgagcggac actgttgcca tccatgagca cggtgcacgc accgcattgc
     7201 cccagaccgc agccaaatcg cgggccatgc aattcaagat tatcgcgcag gacgtaaagc
     7261 aacggtgtgc tgggatcggc ttcaacggct ctcgatttcc cattcacttt cagcgagatt
     7321 cgcgccatgg tgaaattcct ttctcaacgg gtcagtcaga atctatgatt tccaaacagg
     7381 cgattcacat attccaacat tatcaatgct acgttaccgc taaatataca cccggtcgcg
     7441 gtgagaagga aatgcccagg ctcaatttga aattgggatg gggtaacgaa tggttgaatg
     7501 ttcgatccct tgcaaaagcg tgcgtgttta aatcgtttac cggttaccgg gcgtattcgt
     7561 cagccgcgtc gaacacatac ctgatcgtga tgggcgctcc ggatttgcca ttttcaattc
     7621 gccaggtttc ataaacttgc cggccatccg ccgatagctc aatcgtccgg ctcattttct
     7681 tgttttgcgg acccttttca tccgtcacaa gtttcgggcc atcccaatga gcgggaattt
     7741 catgcagggc ttcatttttc gatttctgaa ttttcctgcc gtcggtgaaa aacaccaact
     7801 tgtcgccatg atcgtttagg gaatccactt cggcattggt caagctgata ttctgcgtat
     7861 aatcggggcg aatctgttcg aaaattcgtt ggctggtttc gctgtctccc ccgcccattc
     7921 cgcggccact gccgccatag gggccgtttc cgccgctgcc gggaattggg aaaccaacac
     7981 ccacgcgtcc gttgcctggt atgccggggt ccacaggtcc gttgccgccc cccgcctttg
     8041 ttcgccgcgc ttcgtccagc ttatttctcg catcgtcgct ttcgtccata ttcaatttcc
     8101 agaatcccgc cagtgttttc cgcggcggaa cctcggtctt ctttttggcc gggggcgcgg
     8161 tttttggtgg aggcagtggc tgcgaaggag acatggggcc gggaggggcc tgcgcgaaga
     8221 cgcgtccggc aggaaaaaag aaaaggccgc atagaaatgc gaagcaagtc gtggatttaa
     8281 ttttgctgaa catcataagt ttcttgtctc cgggaagcat gacgcttacg gtccagccag
     8341 atagatgaca aaagtttcag atcgagttgc ttcgtcgaag actctgtgaa ttatacctca
     8401 gtcgcgatag cgcagctgct gtagcccgcc tcttcagagg cgggggttta gattccccgc
     8461 tggcaggctc gacgtgtaaa ctctaaaccc ccgcctctga atgaaaaggc gggctacagc
     8521 agcccgaacc atcttgtgga agccaacaga gccatataag tcca
//
LOCUS       DASZMB010000180        10053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4321350, whole genome shotgun sequence.
ACCESSION   DASZMB010000180 DASZMB010000000
VERSION     DASZMB010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 10053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10053
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4321350"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VG272_08140"
     CDS             <1..344
                     /locus_tag="VG272_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13688.1"
                     /translation="PPSLFAQLPEMQLFSFFFETSESRRKQLNIKTVYNNGNFAMAVD
                     HTLFKVRLNSAGQPDFEHVAPNNKTEHWYLDETGKLAVVREVTYARVFVKKDGKWRPA
                     VGASATLPMSK"
     gene            complement(381..2189)
                     /locus_tag="VG272_08145"
     CDS             complement(381..2189)
                     /locus_tag="VG272_08145"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWF13689.1"
                     /translation="MRRVLVILTLLVIAPVSCRSVLAQGVTDLRAFEKRCTSCHGNPS
                     IAQAPDGLALRKLTPEAVYAAITTGSTHAKLDVSEEEKRMIAGYLGGRKVDAARLTDA
                     KLMSNPCPANPVIKSLASEPSWNGWGADLTNARLQSAKAAGMTADDVPKLKLKWAFGL
                     PAAEIMWAQPTIAAGRVFVGADSGAIYSIDQATGCVHWSFQADAGVRNAITVRPIKGQ
                     GAAKFAVYFGDIKANVYAVDALTGKQLWKTKVEENPVARITGAPIAFEDRLYVPVAST
                     EERAAGYSTTYPCCTFRGSVAALDANTGKQIWKTFIIPDEPQPRKKAASGVQLWGPAG
                     GAVWDSPTLDPAHHALYIGTGDAYTDTEQPIKTTDGIMAINMDTGKVLWSAQDTANDI
                     WLAGCGPQNTSENCPKDIGPDYDFGSSPILRTLPNGHRILVAGQKNGIVWAHDPDEQG
                     AVVWKVPLVDKAMRGVITFGGAADEQNVYFGLSTGGIAAVQLSNGEKKWFAPIAAAQE
                     GRGGGQSAAITAIPGVIFSGGWDGMLRAFSTADGHPLWQYDTTQEVKTVNGIPAKGGS
                     MGAPGPTVAGGMLFVGSGYTFGAGATGNLLLAFSVQ"
     gene            complement(2260..2817)
                     /locus_tag="VG272_08150"
     CDS             complement(2260..2817)
                     /locus_tag="VG272_08150"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HWF13690.1"
                     /translation="MKKRLFSAICTAAATALLIAISGPAMAQTAQVLPIPEAPVAANI
                     PSAHELPDPNLTYRIAFGIKNPAPKIDEINPGLISVARYFNTLAMNGVPADHRKIVVV
                     FHGAPIFLNNDAYKARNEGHDNPNIALIRSMKKAGIDFRVCGQELLAMKIDLESVLPE
                     VQIDLWAMTTVTNLQTRGYIYIAGN"
     gene            complement(2996..4351)
                     /locus_tag="VG272_08155"
     CDS             complement(2996..4351)
                     /locus_tag="VG272_08155"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HWF13691.1"
                     /translation="MNESSFVSNPVGTDSEMLDHLRRETFDYFRHEFNPQNGLIADKT
                     QPGSPSSIAVMGMGLSVFAVAVERGLLSRAEAIDRTLTILKFFHSSPQGPEPDATGYK
                     GFYYHFLDMHTGRRAWRCELSTIDTAIFMAGVLTAASYFTDYNDKETEIREKAEALYR
                     RVDWRWALDHGATIRHGWKPETGFLPYRWDTGYSEALILYVLALGSPTFPIDPKGYTD
                     WTSTFLWKRVYDIEYLYAGPLFIHQMSHLWLDFRGIHDDFNRKTAIDYFENTRRATYV
                     HRRYGIENPMGFLHYHKYNWGLTASDGPGPAILDVNGIRREFFDYIARGAPFGPDDGT
                     VSPWAVVASLPFAPEIVIETIRHAIEHFHLKKRSPYGFDASFNSTFPDTTRNQHGWVS
                     PWIFGLNQGPIILTTENFQSELIWKTIRKCPFIATGLRRAGFRDGWLNHLEHKDSGTV
                     R"
     gene            complement(4476..4628)
                     /locus_tag="VG272_08160"
     CDS             complement(4476..4628)
                     /locus_tag="VG272_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13692.1"
                     /translation="MWKRVAGISAIERLDEDHSNRQRRWQSMGELEYLSAVQVERLTT
                     ASVLKK"
     gene            5463..6173
                     /locus_tag="VG272_08165"
     CDS             5463..6173
                     /locus_tag="VG272_08165"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HWF13693.1"
                     /translation="MTPAYEGADSACEERIKAAGFILAGGTSSRMGRDKALLELDGVP
                     LVVRTARLFEVLVADVTVVGPREHYAPFGLRGIADYSRDSDAEGQREGPLAGIIAALT
                     NSAAPWNLIVACDLPYLSHDWLNWILCRAASSEKLAVVPCAGRGPEPLAAVYRRECGP
                     LLISAFSAGIRKVMEALSDLDVEFVDSAEWHSVDPTGKVLTNMNVYEDYLEAKTHYEL
                     RSGGNKTISPPTQRERKY"
     gene            complement(6348..6725)
                     /locus_tag="VG272_08170"
     CDS             complement(6348..6725)
                     /locus_tag="VG272_08170"
                     /inference="COORDINATES: protein motif:HMM:NF014671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HWF13694.1"
                     /translation="MGNVIQIRLGPEANIPDVETRGFSAGSACGVCGKASIERIRNRN
                     IGRPESGTRFEPAMLCTLPEKLCAAQPIFGRTGGLHAAALFDSHGEMVVLREDIGRHN
                     AVDKVIGWALNEGKMPLKDMLCW"
     gene            complement(6738..6974)
                     /locus_tag="VG272_08175"
     CDS             complement(6738..6974)
                     /locus_tag="VG272_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HWF13695.1"
                     /translation="MNQKRSVSSTQVTEWKNGEARRTDDFLAGEEPLEMRIGNRAYGV
                     TMRTTGNDRELVAGLLFTEGVISCREQLLELDVD"
     gene            complement(7175..8530)
                     /locus_tag="VG272_08180"
     CDS             complement(7175..8530)
                     /locus_tag="VG272_08180"
                     /inference="COORDINATES: protein motif:HMM:NF037981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HWF13696.1"
                     /translation="MAVNSSTSEAASREFFDVGINDFLPPGQLLILGLQNVFGMTGMF
                     VFPGLLGRSFNLPPEKIAYLYAMTFAVCGVITVLQSIFLLRLPIVQGPYSGSFAALIS
                     VGHLQNGGLGAAYGSFFVASLIWCALTLPVRRFSVIGLFARYLRAPIISGMIVMLVII
                     QISSVALPNWIGLPSSAGFPIINILSGAVAIAIVIGVTLWGGPRLRRGAILAGLALGT
                     TCYAIFRPISFAAVASAPVLVAPHWFPFGFGVRADLVLVFLLVLIPPGMGSMAMYQMV
                     ADWGNEKLSSKRMAEGVFGMAIGSVLAAVVGGLSTIAYPDNIGMLRATRVGSRYATLA
                     AGILLIALGGFIKFDMLLVVVPTAVISATATLLFGMVFMHGVHMLANVEWDERKFMVA
                     GLALLVGLGGLFISQDALQGMPLLARLVIQQPVISGGLTLVMLHSILCSIPHAQAGTR
                     P"
     gene            complement(8606..>10053)
                     /locus_tag="VG272_08185"
     CDS             complement(8606..>10053)
                     /locus_tag="VG272_08185"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HWF13697.1"
                     /translation="SHGRRYDIPVVVGALAASPKIYAAGMGCAVEEIGAAWDHAVANP
                     IPPSLVNSAPCQEVIIRGDELKKPGGGLARLPVPISTPGFDAAPYLTATLCITRDPET
                     GIRNMGTYRGALKATDRLGVRMSSRIGGAGGYLHWLKYQKRKEPMPCAIVIGCAPVVV
                     YTGPQKFAANVDEITIAGGAAGAPIRTVKCVTLDLEIPADSEIVIEGLIDTELLEPEG
                     PFGESNGYVALEDYNMIMQVTAITHKQRPIFASILSQVTPSESSVIKKVAYEPAFLAH
                     LRDVLSIKGIRRVVMHEPLSNVRPIIFLQFESDTPRTEVWRGLRGASAKQADCGKIVI
                     AVSRDIDPSNTDAVLWSLAYRSNPAEDVQIEPYRSRGHAPKSGSQVLDSTMLIDATLK
                     GSMPPLALPARESMERAQEIWKELELPPISVRAPWHGYSLGDWSDAWDHFARNAVAGA
                     WEENGANTATRRRGGLTPETPVRNIEGRDKS"
BASE COUNT         2265 a         2836 c         2691 g         2261 t
ORIGIN      
        1 ttcctccatc acttttcgct cagctcccgg aaatgcaact tttttcattt tttttcgaaa
       61 cctcagaatc gcgcagaaag cagctcaaca taaaaacggt ctacaataac ggaaattttg
      121 ccatggcggt ggatcacacg ctattcaaag ttcgactgaa tagcgctggc cagcccgact
      181 tcgaacacgt ggctcccaac aacaaaaccg agcactggta cctcgacgag actgggaagc
      241 tggctgtcgt acgagaagtc acgtatgccc gggtattcgt gaagaaagat gggaagtgga
      301 gacccgctgt cggtgcctcg gccactcttc ctatgtccaa ataaccgctg cgccgccaag
      361 atgcagcatg tttttcgagt ttattgaaca gaaaatgcca gcagcaagtt accggttgcg
      421 ccggcgccga atgtgtatcc cgatcccacg aagagcatgc cgcccgccac ggtggggccc
      481 ggagctccca tcgaaccgcc ctttgccgga atgccattca cggtcttgac ctcttgcgtt
      541 gtatcgtact gccagagagg atggccatcc gccgttgaaa acgcgcggag catgccgtcc
      601 cacccgccgg agaaaattac tccggggatt gccgtgatgg ccgcggattg gcccccgccc
      661 cggccttctt gcgcggcggc gatcggcgcg aaccatttct tttcgccgtt cgataattga
      721 acagccgcaa tccctccggt actcaggcca aagtacacgt tctgttcatc tgcggctccc
      781 ccaaaagtaa ttacgccgcg catcgcttta tccacaagcg ggactttcca gacgaccgcg
      841 ccttgttcat ccggatcgtg cgcccagaca attccattct tttgtccggc gacaagaatc
      901 cggtggccgt tgggcagcgt ccgcaaaatc ggggatgagc caaagtcata gtcgggacca
      961 atatccttcg ggcagttttc agaagtgttt tgcggcccgc atccggcgag ccagatatcg
     1021 ttcgctgtat cctgcgcgct ccaaagaact ttgcctgtgt ccatgttgat ggccatgatg
     1081 ccatccgtcg tcttgatcgg ttgctccgta tccgtgtacg cgtcgccggt tccgatgtat
     1141 agcgcatgat gcgcgggatc gagagtcggc gaatcccaca ctgcccctcc cgcggggccc
     1201 cacagctgca cgcccgaagc ggctttcttc cggggctgcg gctcgtctgg aataatgaag
     1261 gtcttccaga tttgcttgcc tgtgttggca tcgagcgcgg cgacgcttcc gcgaaatgtg
     1321 cagcatggat acgtggtgct atatcccgcg gcgcgctcct cagtggaagc gaccggaaca
     1381 tacaagcggt cttcgaatgc gatgggcgct cccgtaatgc gcgccaccgg attttcttcc
     1441 acttttgttt tccagagctg cttgccggtt aacgcgtcca ccgcgtaaac atttgccttt
     1501 atatcgccga aatagactgc gaatttcgcg gcaccttgcc ccttgatagg tcggacggtg
     1561 atggcgttgc gcactccggc gtccgcctga aaagaccagt gtacgcagcc ggtcgcctgg
     1621 tcaatggagt agattgcgcc gctatcggct ccgacaaaca cgcggcccgc ggcaatcgtg
     1681 ggctgcgccc acatgatctc cgccgcgggc aggccgaaag cccatttcag cttgagcttc
     1741 ggaacatcat cggcagtcat tcctgcagct ttcgcagatt gcagccgcgc attcgtgaga
     1801 tccgcacccc agccgttcca cgacggctcc gacgccaaag atttgatcac tggatttgct
     1861 gggcacgggt tcgacattaa tttcgcgtca gtcagccgcg ccgcgtccac cttgcggccg
     1921 ccgaggtatc ccgcgatcat gcgcttttct tcttcggaga catccagctt cgcgtgcgtg
     1981 cttccggtgg tgatcgcggc atacaccgct tcaggagtga gcttccgtag cgctaatccg
     2041 tcaggagctt gggcgatact cgggttgccg tggcaagacg tacagcgctt ttcaaaagcg
     2101 cggagatcgg tgacgccctg tgcaagcacg gaacgacatg aaaccggcgc gatgaccagc
     2161 aacgttaaga tcaccaagac gcgtctcatc ctaatgcctc cgattcactt tcgtccgtca
     2221 gctgcgcgat cacggatttt atcgaggtgt ggaaactctc taattccctg cgatataaat
     2281 ataaccgcga gtctgcaaat tggtgaccgt cgtcatggcc cataggtcga tctgtacttc
     2341 gggcaacact gactcgagat cgatcttcat cgccagaagt tcctgaccgc agacgcgaaa
     2401 atcgattccc gccttcttca tgctacggat taaggcaata ttcggattgt catggccctc
     2461 gttacgcgcc ttgtaggcat cgttgttgag gaaaattggg gcgccgtgga atactacgac
     2521 gatcttacga tgatccgcgg gcacgccatt catggccagc gtattaaaat accgtgccac
     2581 ggagatcagc ccgggattta tctcgtcgat tttcggcgca ggatttttga tgccaaatgc
     2641 gatcctatat gtcaaattcg ggtcgggcag ttcgtgagcg ctgggaatat tggcggccac
     2701 cggagcttcg gggatcggta aaacctgcgc agtctgtgcc atcgccggac cagaaattgc
     2761 aattagtaag gccgtggccg ccgccgtgca aatggcgctg aataatcgtt tcttcatttg
     2821 agcctccggt ccgcgaaatt tgcggatcga cgcagtagaa ttttcaaact ggagcacgcg
     2881 ttggcagcat cgtatccttc cccaaagatg ttcacaataa ttttgtagcc cgcgcaggcc
     2941 acatcgaagt cagctcgcgg ttttcagcat ggaggaaatc aaacgtatct gtttgtcatc
     3001 gcacggttcc gctgtcttta tgctctaaat ggttcagcca accgtcgcga aatcccgcgc
     3061 ggcgcaagcc cgttgcgatg aaaggacatt tccggatcgt cttccagatc agctccgact
     3121 gaaaattttc agtcgtgaga ataatcgggc cttgattcag tccgaagatc cacggagaaa
     3181 cccagccatg ttgatttctg gttgtatcag gaaaggttga attgaaactt gcgtcaaatc
     3241 catacgggct gcgcttcttt aaatggaaat gttcaatggc gtgacgaatc gtttcgatga
     3301 caatttcagg agcaaatgga agtgaagcca ctactgccca cggagaaacc gttccgtcat
     3361 ccgggccaaa tggcgcgccg cgcgcaatat agtcgaaaaa ttcccgccgt attccgttca
     3421 cgtcgaggat cgcgggccct gggccatcac tcgccgttag tccccagtta tatttgtggt
     3481 agtgcaaaaa tcccatcgga ttttcgattc catatcgtct gtgaacgtag gtagcccgcc
     3541 gggtattctc gaagtaatcg atcgcggttt ttctgttgaa gtcatcgtga atgccgcgaa
     3601 aatcgagcca caaatgagac atctggtgaa tgaacagcgg tccagcgtac aaatattcta
     3661 tgtcatagac tcttttccat aaaaaggtgg aagtccaatc ggtatagccc ttaggatcga
     3721 tcggaaatgt tggtgagccc aaagcaagca cgtagagaat gagagcttcg ctgtagcccg
     3781 tatcccaccg gtacgggaga aacccagttt caggtttcca cccgtggcgg atagtagcgc
     3841 catgatccag ggcccatcgc caatcgacgc ggcggtaaag cgcctccgcc ttttctcgta
     3901 tctcggtttc tttatcgttg taatcggtaa aatagctcgc cgcggtaagc acacctgcca
     3961 tgaaaatggc ggtgtcgatc gttgacaatt cacatcgcca ggcccggcga cccgtatgca
     4021 tatccaaaaa atgataatag aatcctttat atccagtcgc atcgggctcc ggcccttgag
     4081 ggctggagtg aaagaatttt aaaatcgtta gagtcctgtc gattgcttcg gctcgtgaaa
     4141 gtaagcctct ttcgactgct accgcgaaga cgctgagccc cattcccata accgcaatac
     4201 tagagggcga ccccggttga gttttatccg cgatcaggcc attttgaggg ttgaattcgt
     4261 ggcgaaaata atcgaacgtt tcgcgacgca ggtggtccag catctccgag tctgtcccca
     4321 ccggattgga aacgaaagat gattcattca ttcctggcga tctccttggt gcaggttcac
     4381 gctcggtcga actccatgct gaccgcagca acactctgcg gcggtaacga caacgcgaga
     4441 gctatatttc cctggtcgtg gacaaatcgt cgcttttatt ttttcaaaac tgacgccgtc
     4501 gttaatcgtt cgacctgaac cgcgctcaag tattccagtt ctcccatgga ctgccaacgc
     4561 cgctgccgat ttgagtggtc ttcgtcaagc cgctcaatgg ctgaaattcc ggcgacgcgt
     4621 ttccacataa ttcatcattt gggacgggac ttccctgccc gctgtttttc gccacggaga
     4681 tctctgtgct ggcccaccta aagcatatcc gctggatcgg atcgccacaa tttctgcgcc
     4741 atcacctcag gcggtccgac atcggtagga cggatcgaaa agatacggcg aacgcgaagt
     4801 ccgaaagcat ggggattgcc aatttgactc ggcctcaaac tggtacagga gctaattcag
     4861 gtgcgtggcg gttgtcgatt cgcgactcgc tgtcacagag gcaggagaat agcatcgagg
     4921 cgagtctgtg gcgagtgatt ggcattcgag aagcgtgcga aggggtttct gatcgactta
     4981 gctattcgac ttggtcttat ccttcgctgc gcggatgcga tcggacaata tgacttttgc
     5041 cttctccagt accctgcgcc gcgattcagt tagtccgctt ccccctcgat tgatgaaata
     5101 agtaagcatt ctcatccccg actgaggccc tttgggcgat acttttttcg aggccaaggc
     5161 cttggctatc gtggccgcat ctttcgtgaa tagtcctgcc tgtggatgcg tcgaatcggt
     5221 ctttactgtt ttgacccatg ttttgctcgg ctttcgcggc atttcggacg tcctttgtga
     5281 ataaattcgg aacgaattat gcgattctat gacttcgaat ctctacgaat actccaagca
     5341 tcctagccat tatttacggc gatcattttt catttcaatc gggtgatcta cggattcgca
     5401 aactgcgatt tgcaattacc ttgtctgaca ctgccacaaa acgggagtga taggatggca
     5461 ctatgacacc ggcttatgaa ggagctgact cggcttgcga ggaaagaatt aaggcggcgg
     5521 gttttattct cgcgggaggg acgagttcgc gcatggggcg agataaagcc ttgctcgaac
     5581 tcgatggggt gcccctagtc gtacgcacgg cccgcttgtt cgaagtctta gtcgcggatg
     5641 tgacggtcgt aggtcctcgc gagcattacg ctccgttcgg gttacgcgga atcgcggatt
     5701 attccagaga ctcagacgcc gaagggcaac gagaaggccc gcttgccgga atcatagcgg
     5761 cgctcacaaa ctcggcagcg ccgtggaacc ttatcgtagc gtgtgacctg ccgtatcttt
     5821 cgcatgactg gctgaattgg attctttgca gggcggcatc ctcggaaaag ttggcggtcg
     5881 ttccctgcgc cggcagaggg cctgaaccgc ttgccgccgt ctaccgtcgt gagtgtggcc
     5941 cgttgctgat ctctgcgttt tcagcgggca ttcgcaaggt tatggaagcg ttgagcgatc
     6001 tcgacgttga atttgtggat tcggctgagt ggcattcggt cgatccaacc gggaaggtac
     6061 tgacgaacat gaatgtttat gaagactatc tggaggccaa gacgcactac gagttgcgga
     6121 gtggggggaa taaaactatt tccccgccga cacagcgcga gcggaaatac tgaggcgttc
     6181 ctcacccgcg taaatcacaa atcgctcccc gcgcagaaat ccaacgagtg tgagacccaa
     6241 ctcccgggcg agctgcacgg caagactgga cggcgcggat acggaagcga gcagcggaat
     6301 gccagccacc agcgcctttt gtataatttc gaacccgcca cgcccgctta ccagcagagc
     6361 atatccttta agggcatttt gccttcgttt aaagcccagc caattacttt gtccacagcg
     6421 ttgtgacgtc caatgtcctc gcgcaagacc accatttcac cgtgtgaatc gaaaagcgcg
     6481 gcggcatgca agccgccagt tcgaccgaag attggctgcg cggcgcaaag tttttccggc
     6541 aacgtgcaga gcatagcggg ttcgaaacgc gtcccgctct ctggacgacc tatatttcga
     6601 tttctaatcc gctcgatcga ggccttgccg caaacgccgc aagcggaacc cgcagaaaat
     6661 ccgcgagttt cgacgtcagg aatgttcgcc tcgggaccga gacgaatttg aatgacgttt
     6721 cccatggacg atgcgtccta gtcgacatca agctccagca actgctcccg acacgaaata
     6781 acgccttcgg taaaaagaag tccggcaact agttcgcggt cattgcctgt cgtccgcatc
     6841 gtcaccccat acgcacgatt cccgatgcgc atttcgagag gctcttcccc agccaggaaa
     6901 tcgtcggttc gacgagcttc gccgtttttc cattctgtaa cttgagtgga ggacacgctg
     6961 cgtttttgat tcatcgatcc tgaacctcgg atcgccgtaa cgacgaccta tgtacatccc
     7021 agtctacaat gcgccgacgc aacggtatcc ccctctggac aggcaagcac agcgacggca
     7081 tcgaacagaa cagatggcga agccctcagc gacattctgc gggcggcaac tttccctttt
     7141 cgtcggcttc ccattggcag ttttgaaacc tgtgctatgg ccgagtgccg gcctgggcgt
     7201 gaggaatcga gcagagaatg gaatgcaaca tcacaagtgt gagcccaccg gaaatcacgg
     7261 gctgctgaat aacgagcctt gccaatagcg gcatgccttg caaggcatcc tgtgaaataa
     7321 ataatccacc gagtccgacg agaagtgcca gccccgccac cataaatttt cgttcgtccc
     7381 attccacatt tgcgagcata tggacgccgt gcatgaaaac cattccaaaa agtaaagttg
     7441 cggttgcgga gattacagcg gtggggacca cgaccagcaa catgtcaaac ttgatgaagc
     7501 caccgagcgc gatcagtaga attccggccg cgagtgtcgc atagcgcgag ccgacgcggg
     7561 tggcgcgcaa catgccaata ttatccggat acgcgatagt gctgagccca cctacaaccg
     7621 cggccagcac ggatccgatc gccattccga aaaccccctc cgccatgcgc ttggatgaaa
     7681 gtttttcatt tccccagtcc gcaaccatct gatacatggc catcgatccc atcccggggg
     7741 gaatcagcac gagcagaaaa acgagtacga gatccgcgcg aactccgaat ccaaagggaa
     7801 accagtgcgg cgcgaccagc actggcgcgc tggcaacggc agcgaatgaa atgggcctga
     7861 agattgcata gcacgtcgtc cccagcgcga ggccggccag aattgctccg cggcgcagac
     7921 gtggtccgcc ccaaagcgtc actccaatca cgattgcgat agccacggct cctgagagaa
     7981 tatttattat gggaaatccg gcactggacg gtaagccgat ccagtttggc aacgcaacac
     8041 tcgagatctg aatgatgacg agcatcacaa tcattcctga aatgatgggt gcgcgaagat
     8101 accgtgcgaa caggccgata acactgaagc ggcgcacggg caatgtcagc gcgcaccaga
     8161 tcagcgatgc aacaaagaaa gatccatacg ctgcgcccag accgccattt tgcaaatgcc
     8221 cgacggagat caaagcagcg aagctgccgg agtacggtcc ctggacgatt gggaggcgaa
     8281 gcaggaatat ggattgcaaa acggtgatga cgccgcacac ggcgaacgtc attgcataaa
     8341 gataagcaat tttttccggc ggcagattga aggatcggcc aagtagcccg ggaaatacga
     8401 acattcccgt cattccaaaa acattctgca aaccaagaat cagaagttgg ccgggcggaa
     8461 ggaaatcatt gataccaaca tcaaagaact cgcgcgacgc tgcttccgat gtcgaagagt
     8521 tgaccgccat aaaattcctt ttcgactgaa agatcgaatt actttttcgg actctaatac
     8581 agataggatc gccgaaatta cgctgctacg atttgtcgcg cccctcaata ttccgaacgg
     8641 gggtttcagg cgttaggccg cctcgccgcc gcgttgcggt attggctcca ttttcctccc
     8701 acgcgccggc aacggcgttc cgcgcgaaat gatcccacgc gtcgctccag tcgcccagcg
     8761 aataaccgtg ccagggggca cgaacagaaa tcggcggaag ttccaactcc ttccagattt
     8821 cttgtgcgcg ctccatcgat tcgcgggcgg gcagcgccag cggcggcatc gaacctttta
     8881 aagtagcgtc aatgagcatt gtcgaatcca aaacttgtga accggacttt ggcgcatggc
     8941 ctcgcgagcg gtacggttcg atctgcacat cttcggcggg atttgagcgg taggctagcg
     9001 accacaacac ggcatccgtg ttcgaagggt cgatgtcgcg gcttacggca atcacaattt
     9061 ttccgcagtc ggcttgcttc gcggaagcac cgcgcaatcc acgccacact tctgtgcgcg
     9121 gcgtatcgct ctcaaattgt aagaaaataa tcgggcgaac attgctcagt ggttcatgca
     9181 tcaccacgcg gcgaattccc ttgatcgaca acacgtcgcg caaatgggcg agaaacgcgg
     9241 gttcgtaggc gactttttta atcacgctcg actcgctcgg cgtgacctgg ctgaggatgg
     9301 acgcaaaaat gggcctctgt ttgtgggtaa tggccgtgac ttgcataatc atgttgtaat
     9361 cttccagcgc gacgtacccg ttgctctcgc caaacggccc ttccggctca agcaattcgg
     9421 tatcgatcag cccttcgatg acaatctcgg aatccgccgg gatttccaaa tcgagcgtca
     9481 cgcactttac ggtgcgaatc ggcgcgcccg ccgcgccccc tgcaatcgta atttcatcca
     9541 cgtttgcggc gaatttttgc ggtcccgtgt aaaccactac gggtgcgcaa ccaatcacaa
     9601 ttgcgcacgg catcggttct tttcgcttct ggtatttcag ccaatgcagg tacccgcccg
     9661 cgccgccaat tctcgacgac atgcgcacgc caagtcggtc cgttgctttc agtgcgccac
     9721 ggtacgtgcc catgttgcga atcccagttt cagggtcgcg cgtgatgcaa agagttgccg
     9781 tcagatacgg agccgcgtca aaacccggag tggatatcgg cacgggaagc cttgccagac
     9841 ctccgcctgg ctttttcaat tcatcgccgc ggatgattac ttcttggcag ggcgcggaat
     9901 ttacgaggga gggcggaatc ggatttgcga cggcatgatc ccaggcagcg ccaatttctt
     9961 ccaccgcgca tcccatgccg gccgcgtaaa ttttcggtga agcggcgagt gccccgacga
    10021 cgacgggaat gtcgtatcgg cgcccgtgcg aat
//
LOCUS       DASZMB010000181        31014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4591917, whole genome shotgun sequence.
ACCESSION   DASZMB010000181 DASZMB010000000
VERSION     DASZMB010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 31014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31014
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4591917"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VG272_08190"
     CDS             <1..610
                     /locus_tag="VG272_08190"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HWF13698.1"
                     /translation="AGKDMNNSIESNGVVKNDAVNLVYTRLADDIPNDPSKLSAYFAK
                     GGKLLIYHGFDDLIISPYSSIWFYEDLAQENGGYPKLQQNARLFMVPGMQHCVLGAGP
                     NSFDTLSALEQWVEKGIAPDAIPATHSTNNAVDRTMPLCKFPEQAHYKGSGDPKSVAS
                     WSCKPDDKSLLAVGPNGTQAGANATPPARIRPAALLATKSGN"
     gene            complement(911..1390)
                     /locus_tag="VG272_08195"
     CDS             complement(911..1390)
                     /locus_tag="VG272_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13699.1"
                     /translation="MQCAPPGVPRIYQMRGEPMEIAHIPGRVLMIFEYDHFIRNIFTD
                     GRAHPKDLSPSWMGDAIGKWEGDTLVVDTVGFNDKTWLDYDGLPHSEDLHLVERIRRV
                     NHDTLTIDITIEDPKAYTKTWESHAVYELKPTWNLGEVVCEDNITYGDMQQKSEGTK"
     gene            complement(1725..2123)
                     /locus_tag="VG272_08200"
     CDS             complement(1725..2123)
                     /locus_tag="VG272_08200"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HWF13700.1"
                     /translation="MTTKRSIFLLLAFGFLFSSAPVFAHHGASVYDSAKLTTLKGIVT
                     DFQFMNPHTEILFDVAGADGKVEKWTAEAPSLTTMYRGGWNKNSMKSGDKVTFIGNLA
                     KNGSHIMRIRKVIFPNGKETVMDREEDYAE"
     gene            complement(2210..2920)
                     /locus_tag="VG272_08205"
     CDS             complement(2210..2920)
                     /locus_tag="VG272_08205"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWF13701.1"
                     /translation="MQTLDSIRARIFPRPLLAIFIIAILAASIFTQSAVNAAPSPADK
                     RKPGLYWTLETDKGKISCKLFEAEAPVTVRTMVGLAIGKISYVHPETKQTERKKFFDG
                     LVFDRVIPGVMIQGGDLLGTGVGHPEGPGFPYKSEIVPSLKFDTPGRLAMANSGPDTN
                     DTHFFITEAPFPSFNGKYTIWGQCENVDIVKTIAQVPRDPGDTPITPVHIQHVTIERV
                     GPTPSDAPEALPVDNPSK"
     gene            complement(3086..4156)
                     /locus_tag="VG272_08210"
     CDS             complement(3086..4156)
                     /locus_tag="VG272_08210"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HWF13702.1"
                     /translation="MREVPRFFSSLALSLLLLSIVSGSAHAQASYSPPNDLPNPYRTI
                     SNWAKLPDGRKWGATSGVAIGPDGNIWTYERCGGNSCADSNVPPILEFDPSGKILKNF
                     GAGLFVLPHGIFVDKDGNVWVTDGVGGGGTSDDVGKHGKGHQVFKFSPNGKVLMTLGK
                     AGAPGATHDTFNRPSAVLVAPNGDIFVADGHNSPPNSQMNARIVKFSKDGKFIKEWGK
                     PGTGPGEFNTPHALALDSKGRLFVADRANNRIQIFTQDGKFLNEWKQFGRPSGIFIAS
                     DDTMYVVDSESTDQPGYGYNPGCKRGMRIGSARTGKVAAFIPDPAPGGSTSAAEGVAV
                     DRNGNIYGAEVGPMDLKKYMKK"
     gene            complement(4331..4786)
                     /locus_tag="VG272_08215"
     CDS             complement(4331..4786)
                     /locus_tag="VG272_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13703.1"
                     /translation="MKFQFLKTALFTMAILLTASAASTGEGVPAAVHFIPHDEVATVM
                     AKGGPILNDPGLLILANRRAAGAVEYHEHTNHIFIMVDGEATFIAGGTMIGVKRTSPD
                     QMTATSIEGGQTFHLTAGDVITIPAKTPHWWKELSTKTVGYYAVNIESK"
     gene            complement(4856..5383)
                     /locus_tag="VG272_08220"
     CDS             complement(4856..5383)
                     /locus_tag="VG272_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13704.1"
                     /translation="MRTLGISVVLALIAAVVVMARQPAAVPQAPAAQMSAAPMKTFSS
                     AADVQALLAKAKSERKEGQVMVAEPILSLSPYTTNLEYRPVEGAVAIHEKEAEIVYVI
                     DGGGTLTTGGTVVGEKRTNATNLSGASIDGGTIQQIGKGDFTIIPEGTPHQFKPADGP
                     LVLMTQHVPRPVAAQ"
     gene            5577..5927
                     /locus_tag="VG272_08225"
     CDS             5577..5927
                     /locus_tag="VG272_08225"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HWF13705.1"
                     /translation="MVKLLAHWILSALCLLLVAHFVPGFYVSGFATALIAAVIIGLVN
                     GTIGMLLKILTFPLTILTFGIFWLVINALMLKFAALFVPGFEVRGLWPAFWGGLILSI
                     LNMIVRHFLKKSNE"
     gene            complement(6053..6382)
                     /locus_tag="VG272_08230"
     CDS             complement(6053..6382)
                     /locus_tag="VG272_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13706.1"
                     /translation="MSHEIIVQFLGYQVKPLEREYKFAVREVSTEPREFTLTIPAAAF
                     NERLLRIQDAPDVCSRKLRRELEANANHPPETHYAITNAELEDYRIARAPRTPKSPFS
                     RKPVEDY"
     gene            complement(6379..6636)
                     /locus_tag="VG272_08235"
     CDS             complement(6379..6636)
                     /locus_tag="VG272_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13707.1"
                     /translation="MKALEEGAYIKHFQYGLGVVTESNSDRTSIDFDVHGMKKFVTSL
                     MVVDLAEGTPPRRRRTKKTKKTAAVAPTAETPVIGLAAGAK"
     gene            complement(7226..8290)
                     /locus_tag="VG272_08240"
     CDS             complement(7226..8290)
                     /locus_tag="VG272_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWF13708.1"
                     /translation="MRKTLLLAMVIAIGLLSPALKAQQKKALEAYFIDVEGGQSTLFV
                     SPSGQSLLVDTGWAGARDSGRIVSVAKMAGVSQIDYLVLTHYHGDHAGGVVDLARAIP
                     IKNFVDHGPSAEATLNVPQTYAAYLTVRGEGKHIQVKPGDKVPIVGLDVQVISAAAET
                     ISKPLPGAGAANPDCGGFVPKDEMKETLVGGENKQSVGMVISLGKFRIADFGDLTWNK
                     EHDLACPKNLIGTIDAYVVSHHGQDISSLPMLVHAMRPRVAVMDNGANKGGAVATFET
                     LKSSPGLEDLWQLHFAEGAGGHNMPEKFIANQGTGGTAQTGVPNEGPVNYIQLTAQPD
                     GSFSVKNSRNGFQKDYPAKH"
     gene            8483..9850
                     /locus_tag="VG272_08245"
     CDS             8483..9850
                     /locus_tag="VG272_08245"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HWF13709.1"
                     /translation="MSRNLTRSRIVATAARLALLFFAVAICAAAHDIPSDATVQMFFK
                     PDGNHLNILMRVPLKTMRDVEFPEQGQGYLDFERVDPALHEAATLWLSDFIDVYEGDT
                     RLPKPRVVETRLSLESDPSFASYEQALAHLSDPRLTNKTPIFWDQLMMDVLFEYPIQS
                     DQSHFSIHPGLDRLASRIVIALRFLPPGGAVRAFEFLGDPGLVHLDPRWFQAAGQFVK
                     LGFFHILDGTDHLLFLFCLVIPFRRFRALIPVVTSFTIAHSITLIASAYNLAPDALWF
                     PPLIETLIAMSIVYMALENIVGGATVQRRWMITFGFGLVHGFGFSFALRQTMQFAGSH
                     LLASLLSFNVGVELGQLLVLALMIPALELLFRRVVAERMGTIILSAIVAHTAWHWMLE
                     RYQILSQYPIHVPTLEAAPLAKALRLMMLIVIAAGLVWVVFGLLWPRIGKVEQVEEDP
                     AVRLP"
     gene            10036..11382
                     /locus_tag="VG272_08250"
     CDS             10036..11382
                     /locus_tag="VG272_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF13710.1"
                     /translation="MIDVREEIANAPIGRYHRFLALLIGMVVFFDGYDTFNPAYVIHY
                     VAKPWHLAPGQAGLLVSSGLIGFMIGSLLQGKFSDRYGRRTTLLVALWIVTLFSFATA
                     TLGNSFFAFCGLRLLTGLGMGALLPLGVTYVNEYAPRRLANTFSMWGWALGWATGGIV
                     AAAVGVYLTPIFGWQALYYSASLSILLVVICHVALPESLQFSAMRGNWRGIVQVLSRL
                     NPSQASRYSVPDEKFIFPEPSDRPASISLLLSPRYRRTTLSVWASAFFILFGIWGLSG
                     WVPTAMMQRGEGFATSFSFGALIQVMAFLGSLICGYIADRTGRDREAMSVWWFGGAIS
                     VGILVLFNIHALNIICVGAAGFCILGGQNILNNFTAASYDTEVRGTAVGMMLGVGRAG
                     GILGPFITGLLQQRTPGTSGLFVAIGAASFIGGCAILFATSRISRGQIATAEVVRT"
     gene            11585..12346
                     /locus_tag="VG272_08255"
     CDS             11585..12346
                     /locus_tag="VG272_08255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWF13711.1"
                     /translation="MTQSKPAALITGAATGIGRATAVALAGAGYDVAINYSRSEAAAR
                     ETAAQAQAKGAKTLLFQCDVSDDASVRKMLATVEKEFGRLDALANNAGTTSNVKPGDF
                     EGMTAEEWDRVFAVNVRGIFQVTRAAAPMLKAARGSVVNTASIVGLRPGPQPLPYAAS
                     KAAVVSLTKLLALNLAPDVRVNAVAPGWMEGDWMQRMLGERYDDLMARRAKSTPLRRC
                     ATAEDVAEVIVNLITSNRFINGEIIVIDGGFSATT"
     gene            12429..14096
                     /locus_tag="VG272_08260"
     CDS             12429..14096
                     /locus_tag="VG272_08260"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWF13712.1"
                     /translation="MLEGVVPFPPEFAKRYREKGYWIDKTLAQEYRIAFDKYEKRIAL
                     LDRDRSFTYGDLDWMSDNLALNLLEIGFRPLDRLVVQLPNVAEFVVLYLALQKIGGIL
                     IAALMPHRFAEISQFVELSGAVACVVPDEQGDFDYCAMVDRIRQKVSHLRFGIVLGPA
                     QPGFFSLSDLISKPATLPKSALAEIQLDPEDPCIFQLSGGTTGIPKLIPRTHNDYAYN
                     TKMASKVCGVSEDTVLLVILPIAHNLPLACPGIQGVLFNGGRAVLSTSTRPENVCALI
                     EKHRVTHIKVVPALLIRLLHDPAVTRHDLSSLKIIQSGGQSMLPEVRLLSKKLIPSAF
                     VQENFGMSEGLLMFVRLDDPEDVSQETVGRPVSPDDEVRLLADDDREMLGGEVGELCC
                     RGPYTLRGYFGVPEYNARTFTSDGFYRSGDLMRRHKSGNYIVEGRKKDLINRGGEKIS
                     AEEIENLILSHSSVKNASCVPIPDPILGERMCACVLLRENTQLNFDELKQFLLAKEIA
                     KYKLPERLEVMQDFPLSPFGKVSKKSLTEMITQKMVEEQKSKGGASA"
     gene            14244..15086
                     /locus_tag="VG272_08265"
     CDS             14244..15086
                     /locus_tag="VG272_08265"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF13713.1"
                     /translation="MRIIDVHCYPNTREWIACQQPYVDALAKYWNRPWVPKEEDEVVQ
                     EFTQAGVEAILVALDLETTIGTPPCSNDYVSAIQKRHPDRIIQSWAAVDPFKGDDALR
                     EARRAIEDLGMLGFHFHPVMGHFAVNDGRLYPLFELIDALKVPVMIDVGTTGMGAGMP
                     GGMGAKIRHAHPSAIDELAADFPSLTILAAHPGWPWVDEMTAVALHKGNVFWELSGWA
                     PQYFPPQLKKDIRGRLKDKIIFGSDYPSIPYERVLREWAELGYTSEIMEQVFHGNAER
                     VLKV"
     gene            complement(15136..16437)
                     /locus_tag="VG272_08270"
     CDS             complement(15136..16437)
                     /locus_tag="VG272_08270"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HWF13714.1"
                     /translation="MLTRRPFLKLLAGSPLLAAPSFSRATSAAVPSPQDMGAALTQSR
                     DLLIKSGTVIDPSQNLHAQLDVAVKDGKILQVAPDIPVDGTRTLISAKGRIVTPGLID
                     VHAHLFEGIGPTGVNADQYCLGRGVTTAVDAGSAGYFSIAGFRQYVIKPSATRVYAMI
                     DIGARGTLMGLAGNYSNLDWVNPQLTARAADANKPDVVAIKARLSREITGANDLEVLK
                     RALQAAEISHLPLMVHVGDSYSPLPEILKQLRKGDILTHCYTGRPNGPLDANGKILPE
                     MLECRARGVLFDVGDGGPHLSLDVAEKCMQQNFLPDTIGTDLGGLSINGPVYDLVTEL
                     SKFLAIGMTIDQVIERVTLKPTHMFNFGADLGTLRPGTVADITILEARDGSFTFTDST
                     GKKRTGLQKLQSTAAIRAGKAYVNRSDDATNLGRGFDQVPA"
     gene            complement(16543..17580)
                     /locus_tag="VG272_08275"
     CDS             complement(16543..17580)
                     /locus_tag="VG272_08275"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HWF13715.1"
                     /translation="MSLVNRMVVALGILCAALIGIRGVSAFAQADLNSAPNPYRTVEN
                     WARLPEGRTWGQVISADIDRDEKSIWVVERCGGTSCANSNLAPVLKFDASGNLVKSFG
                     VGMIVRPHDLFIDKKNNIWVTDGDAENGKGDQVFEFNQDGKVLLALGKAGIAGDGPDT
                     FNKPSDVLIAPNGDIFVADGHGPGSNARIVKFSPDGKFIKSWGKQGTEPGEFNNPHSL
                     AMDSKGRLFVADRGNNRVQIFDQDGKFLEEWKQFGRPSGVYIDKHDVLYVADSESNMS
                     RNPGVRRGIRVGSVKNGKVTAFIPDPVLNPGQGLEPTNGTAAEGVAADDAGNVYGAEV
                     GPRNFKRYEKK"
     gene            17766..19259
                     /locus_tag="VG272_08280"
     CDS             17766..19259
                     /locus_tag="VG272_08280"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HWF13716.1"
                     /translation="MSQIIEPKTTDRKWLGLYDRPHGDLREFLERAESVGEVTHVKGA
                     NWDLELGALAEIVNHKKSEAPALLFDDIAGYPKGFRVLSGSGNSAKRMALMLGFPTPR
                     TAIDVVQAFRDRMKQHKPIPPVIVKQGPILENIDRDDDVDLYKFPAPRVHERDGGRYI
                     GTEDLVIMRDPEEGWVNAATYRVQVHSKNSTGLWMSPGKHGRQIRDKYFKQGKPCPVL
                     VCCGHDPLLFLAAANEIKFGVSEFDYAGGHRGKPFEVIHSEIHGLPMPAHAEIILEGE
                     LTEGDVHTEGPFGEFTGYYASPSSEQPVIRVRRVYHRNDPILTMATPMRPPSDMSFGR
                     SIVKSGMIWDAIERAGISGVQGVWCHEAGAMRLFNVISVKQAYAGHAKQIGLVAAGCQ
                     AGAYLGRFVVVVDEDVDPTDLFDVVWAISTRCDPALDIEIIKRMWSGPLDPIIPHGSP
                     VNYSSRGLIDACRPFERLKDFPPVAGATAELKARVAAKFADILNRIV"
     gene            19463..20815
                     /locus_tag="VG272_08285"
     CDS             19463..20815
                     /locus_tag="VG272_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid/H+ symport family MFS transporter"
                     /protein_id="HWF13717.1"
                     /translation="MTNAKTINVPELIDAQNIGAFQIRIMILCALAAMLDGFAAQMIG
                     YIAPSLAHDMHLAPATLSRIFAVSLIGLMLGALTFGPIADRFGRRRVIVICTLLFALF
                     TLATAFTHSVNGLIALRFLAGLGFGGVMPNTIALTAEYSPQRRRGTMITIMFCGFPIG
                     ATIVGFAAVPILPAYGWRGVFVLAGVMPLLLVPVLALLLPESIRHLVIHGKENSQVRE
                     LLMCVNPHLVFPSDANFAVHEERAPGLPVMHLFRHGRALATIFIWIAFFVSLLDIYLL
                     SSWLPTVFHNAGITLSLSVVATAVFQGGGVVSSLILGIFIDRFGAFRTVAFIYLLGAI
                     FVALLGHSHSIGFILAAAFFAGAGIVGGQTGTNVLAASLYPTYIRSTGVGWGLGIGRI
                     GSILGPIFGGIMLSLHFPLTTIFLVAAISAFIGGAAIFLMGRSQPSAKAKDILSVATS
                     "
     gene            20944..21912
                     /locus_tag="VG272_08290"
     CDS             20944..21912
                     /locus_tag="VG272_08290"
                     /inference="COORDINATES: protein motif:HMM:NF014867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13718.1"
                     /translation="MKVGIVGAGGVGSASLLSLVMRGIACQVVVLDKNHERAKGVVAD
                     LQYGATLSPAVELLAGDYPDLAGAALVIITAGSNEKAGGATDRNDPAGRLKLLETNAA
                     VYREVVPSIVAVAPEALILVVTDPPDPLADLARQLAGHDRVLSSGTFLDSLRFRLHLA
                     RRLKVNPMYVEAQVVGEHGTSQVFLWSSASVGGTPISKLLEKSGQSLAEFQRSVEQDV
                     RYANIAIIEGTGASQLGIGMITARIADAILRDERAVIPIGSYNSKYAVTISLPSVVGR
                     RGVYQILEPEMSADESQSLQRSAEVLRLARSRILPAAPQSSVRHAG"
     gene            complement(21947..22777)
                     /locus_tag="VG272_08295"
     CDS             complement(21947..22777)
                     /locus_tag="VG272_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWF13719.1"
                     /translation="MKKTFTKIQFLILLVAAAFAARPARAQLMPPNSAGVTMGHIHLF
                     VKDVEAQKHFWTATMGGTVVQNGQLMLIQFPGVYIMLRQADPSGPPSGSIVDHFGFVL
                     KDLPATMAKWKADGVEIEQASNPNQGYVHGPDGIRVEFFGDPSISHAVEMNHIHFYPL
                     DIPAMQAWYAKTFGGIPGKRARVATPGWIDCDDLPGVNLSFSQGKTALAPTKGRSLDH
                     IGFDVIDLEAFVKKLDAQGTKLDEPIRTAPNGTTKVVYLTDPWGTRIELTQNLAPATN
                     "
     gene            complement(22937..24196)
                     /locus_tag="VG272_08300"
     CDS             complement(22937..24196)
                     /locus_tag="VG272_08300"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF13720.1"
                     /translation="MKIFWHRLAYATLAVMAFLAFAAIPCCAQTPANDAMVLIHANMI
                     DGISNAPMMDATVVVVNGHIQSIGHGAASAGGGPVIDLSGHWLLPGFVDAHVHVGNLA
                     DAKRALRSGATTIGEAGLNHFADVGMRELNHKGVADVPDIIAAGYHIQTHPADDYFID
                     FPQDTDLMSGMHGAESARRMVRAMVSRGVNRIKVMATERAGTPDTDPRIRVLTDEELS
                     AVVEEANKTGLWVVAHAHGDEGAAAAVRAGVHAIEHGTYLSEGTMRLMKEKGVYLDPT
                     ITALVDMSEPEGEYDNPILEMRGKAMLPTGREMTAKAWKMGLKIVAGTDTSYFDKNNR
                     TMADEIIELSEAGLGPMDAIKAGTSVSAEDLGVDKRTGSIRIGYEADFVVIDRSPLEN
                     IRHIGDVVMVLNNGRVALNRLNVAPHP"
     gene            complement(24332..25270)
                     /locus_tag="VG272_08305"
     CDS             complement(24332..25270)
                     /locus_tag="VG272_08305"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWF13721.1"
                     /translation="MTRVRVWVAALLLAVPAMLGLPGKAAAQDAKQGNLENILWDVNQ
                     QWLCDGPYQKPYAECVKFRSNYWVPGFFEVQSGGTVRNKEEMVATQAAANPATGVRPF
                     PADLKFVAVYGDVAIATDHTDFQTLRPDGKYAFTADSHCLRVFVKQNGEWRPAAAALV
                     PVIPPAEASAKNSGAKTTKTPDEKLEKELSAIDQKWMESVRTAKTEYLKDMYADKWVE
                     IIGWAPTVVLTKPAALERVAHLNFKPGEGLFADQFKLMSVYGDVALATDRRIRKIADA
                     SGNIVSTPHRALLVFVREKGQWKVAADAVVPTIATE"
     gene            complement(25439..25846)
                     /locus_tag="VG272_08310"
     CDS             complement(25439..25846)
                     /locus_tag="VG272_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWF13722.1"
                     /translation="MNKNSSSIRVVHPDEFSAGTSQTPGSQRLAAISTDLGIQSLLWG
                     GTFLVEPGAQTGIHHHGLQETVAYVLAGESYIQWGERGEHSLTAHAGDFLHVPAWLVH
                     REINRSKDTPFRWVVVRSTSEPIVVNLPADTWG"
     gene            26093..27124
                     /locus_tag="VG272_08315"
     CDS             26093..27124
                     /locus_tag="VG272_08315"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF13723.1"
                     /translation="MIIDCHGHYTSEPPAFLEFRKQQIAAFSNSSAAPALPRISDDQI
                     REKLEPAQLKFQRERGTDLTIFSPRASGMGHHVGDAKLSSAWTRICNDMIHRIVNLYP
                     KNFIGVCQLPQSTGVPPENCIPELERCVKELGFVGCNLNLDPSGGSWKEPPLTDKWWY
                     PFYEKMVELEVPAMVHVTATCNPAFHTTGSHYINGDTTAFMQFLTSDLFKDFPTLKFV
                     IPHGGGAVPYHWGRYRGIAQDMKRPPLTELLLKNVYFDTCVYHQPGIDCMTKVIPSDN
                     ILFASEMVGAVKGIDPETGNYFDDTRRYIDNSTILNASDKKKIYEANALKVYPRLARQ
                     MQKQGIGAN"
     gene            27301..28500
                     /locus_tag="VG272_08320"
     CDS             27301..28500
                     /locus_tag="VG272_08320"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13724.1"
                     /translation="MGKIVLAAGVVHAPQLFVRPPSEDLAQLDADIAAMRLIGKDLDE
                     TRPDVAIVIGNDHMETFFLSSVPTFAIMSGERARAEFARKTYDLPVHQGLAEDLLDKL
                     VNDDFDMAYSQDAVLGHAFAAMYEWVIEGRNIPVVPIFVNAYLPPLPTARRCAQLGKA
                     IAKVVASRPEKVAVIASGGMSHFPGTWKYPAPDFDFDYWAIAQMEKGNHEALLKLTSQ
                     QLDVVGNTEMLSWMIMFGMIGDQPGELITYQPTWHHGHAVMRFLPDKRTGKLSEEGTK
                     GPANYTFKGSEGYEFYKHPSPSAYKLNKILYDLRFKPSLRVRLINDIPGVAAEYGLPA
                     DEAKVLETLQDEDIEKFRNGKVHPLVQAGAHPLGMWMSVIVFHAELRRLRTVADKGDA
                     EKASATQ"
     gene            28711..29733
                     /locus_tag="VG272_08325"
     CDS             28711..29733
                     /locus_tag="VG272_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HWF13725.1"
                     /translation="MPKMRVFQVPKAKDPFQLVEREIPEPATGSVRIKVQACGICHSD
                     EYAKEGLRPGVTYPRVPGHEIAGVIDAIGSGVQGWEVGQRVGVGWHGGHCGYCDSCRR
                     GDFVTCSKGRQITGLTCDGGYTEYMVISGGALAAIPDEISATDAGPLLCAGVTTFNSL
                     RNSGARPGDVVAILGLGGLGHLGVQFASKMGFETIAIARGKDKEQFAKKLGAKHYLDS
                     ESQNVATELEKLGGARAILSTVTSGKAVNAVLDGMGISGKTILVGNPDQPLEITGRLL
                     IAGRRSISGWPSGSPIDSQDTLEFCALTGVRPMTEVYPLERAPEAYDRMLSGKARFRV
                     VLTTGL"
     gene            complement(29846..30109)
                     /locus_tag="VG272_08330"
                     /pseudo
     CDS             complement(29846..30109)
                     /locus_tag="VG272_08330"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
     gene            30310..>31014
                     /locus_tag="VG272_08335"
     CDS             30310..>31014
                     /locus_tag="VG272_08335"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF13726.1"
                     /translation="MRIDVHSHYFPAEYLDRLDLYGGSHVTGLIRKMKMDSPGFSGLD
                     AHLQNMDRSNVEMQILSVSGQLPYFAKENDAVDAARLGNDIYARIVREYPKRFAAFAC
                     TPLPHVQASIDETRRALDELGIVGVTAGTFVLGKTIADPAFDLFFTELNRRKAALFIH
                     PTGGSLGSQLIESTNLAWPVGAPLEDTVCLLQFMQADFPSRFPDVKIIIPHLGGFAPF
                     IAARLDQLQDHFLPASA"
BASE COUNT         6864 a         8450 c         8186 g         7514 t
ORIGIN      
        1 cgccggcaag gatatgaaca actcgatcga gagcaacggc gtggtgaaga acgatgccgt
       61 gaatctcgtt tacacgcgcc ttgcggatga tatccccaac gatccgtcaa aactcagcgc
      121 gtattttgcc aagggcggaa agctcctgat ttatcatggt ttcgatgatt taattatttc
      181 tccctatagc agtatctggt tctatgaaga ccttgcgcag gaaaacggcg gctacccgaa
      241 actgcagcag aatgcgcggc ttttcatggt gcccggcatg cagcattgtg ttctgggagc
      301 gggcccaaat tcgtttgaca ctctgagcgc gctcgaacaa tgggtggaaa aagggatcgc
      361 acccgatgca attcctgcca cacattccac taacaatgcg gtcgaccgca ctatgccgct
      421 gtgcaagttt cccgagcagg ctcactacaa aggcagcggc gatccgaaga gcgttgcgag
      481 ctggtcttgc aagccagacg acaaatctct gctggcagtc ggccctaacg gcactcaagc
      541 cggagcgaat gcaactccac cggcacgcat aaggcctgcg gcattgctag ccactaaaag
      601 tggcaattga atgggattta gtaggacaga ctgaagagcc tgcgtgagag ctgcaagatt
      661 ggaacttcag cggctaaaca gtttgcggaa aaagtagttt attctttcat tccgagcgaa
      721 gcgaggaatc tctcttcgat ttaagtataa gaaaataaag aaggagggat tcctcgcttc
      781 gctcggaatg acaaaatgtt gaggttttcc gcagcctcta aagccggact caatttgcaa
      841 ggcttatgtc gtggctgaag ccacgaccca caatgattct gcagttctca caaaaaccct
      901 gaagcctgtc ctacttggtc ccttcggatt tctgttgcat gtcgccgtag gtaatattgt
      961 cctcgcagac gacttcgccc agattccagg tcggctttaa ttcatagacg gcatgacttt
     1021 cccaggtttt cgtgtaggcc ttgggatctt caatcgtgat gtcgatggtc agcgtgtcgt
     1081 gattcacgcg gcggatgcgc tccaccaggt gcaggtcttc actgtgcggc agcccatcat
     1141 aatcgagcca agttttgtcg ttgaatccaa ccgtatccac gacgagcgtg tcaccctccc
     1201 attttccaat ggcatccccc atccacgagg gcgacaaatc ttttggatgc gcgcggccat
     1261 ccgtaaaaat attgcgaatg aaatggtcgt actcaaaaat catcagcact cgccctggaa
     1321 tatgcgcaat ttccattggt tcaccgcgca tctggtaaat tcgcggaacg cccggcggag
     1381 cacactgcat gatgggatcg tttgattcgg ctaaggagac ggatcttgat ccgacgtttg
     1441 gtttattggc cttgtatttt tcttccgccc atagagtcat cggcggcaca acgttggtaa
     1501 gggaaaatgc gagttcgtaa atggtgtact tgcgggcttc gtcgggcggg cggcgcgatc
     1561 gtctccaaac cctgacaaat ccgcctgtgg cgaaacagta ttggactttt ctcctaccgt
     1621 tatggcagat tgcgcggaaa tcgcaggcac agatccgagc gcagtagcga tcgcgagcaa
     1681 tatgattaaa aaccaaacac gcattccagc ggtcccttca gcgattattc cgcgtaatct
     1741 tcttcgcgat ccattaccgt ttccttgcca tttggaaaaa tcactttgcg gatgcgcatg
     1801 atgtgcgaac catttttcgc gaggttgccg atgaacgtaa ccttgtctcc ggatttcata
     1861 ctgtttttat tccaaccgcc tcggtacatg gtggtcaggc tgggagcttc ggcggtccat
     1921 ttttcgactt tgccgtctgc tcccgcgaca tcaaataaaa tttccgtgtg cggattcatg
     1981 aattgaaaat ctgtgactat gcccttcaac gtagttaatt ttgccgaatc gtacaccgac
     2041 gcgccgtgat gggcgaaaac gggtgctgag gaaaagagaa agccaaaggc cagtaaaagg
     2101 aagatcgaac gcttggttgt catttgatcc ctcgtagttg ccgcgaatat cccactgccg
     2161 ccagggactg tcaatccaaa acaaaatgag agcgtttcac ggaatgaaat tacttggagg
     2221 gattatccac cggcaaggct tcgggcgcgt ctgaaggagt agggcccacg cgctcgatgg
     2281 ttacgtgttg gatatggacg ggagtaatag gcgtgtcgcc ggggtcgcgc ggcacttgcg
     2341 cgatcgtttt cacaatgtct acattctcgc actgtcccca gatcgtgtac tttccattaa
     2401 aggacggaaa gggagcttcg gtgataaaga agtgcgtatc attcgtgtcg gggccgctgt
     2461 tggccatcgc caatcgcccc ggcgtgtcaa atttcagtga aggtacgatt tcacttttgt
     2521 agggaaaacc agggccttcg ggatgtccaa ctcctgtacc gagcaaatcg ccgccctgga
     2581 tcatgactcc gggaatcaca cggtcgaaga cgaggccgtc aaaaaatttc ttgcgctcgg
     2641 tttgctttgt ttccggatgg acgtacgaaa ttttcccaat cgcgaggcct accatagtgc
     2701 gcaccgtgac gggggcttcc gcttcaaaaa gcttacagga tatttttcct ttgtcggttt
     2761 cgagcgtcca gtacaggccg ggtttgcgtt tgtcagcagg ggacggcgct gcgttaacag
     2821 cagactgggt gaaaatagaa gcggctagta ttgcgataat gaaaatcgca agaaggggtc
     2881 gaggaaaaat tcgcgcgcga atcgagtcca gtgtttgcac ttttacttgc tcctaaaact
     2941 tgatgttcta atatataaaa tgctcaacac agaatgcacg gagaaatacg gagtaccaca
     3001 gagcgttcgt cgatttcatt gcggattcta attgccgctc atttgtttta tttgttagaa
     3061 cttgcaaaag aaattgtgac aatttttatt tcttcatgta ttttttcaaa tccatggggc
     3121 ctacttcggc cccataaatg tttccgttgc ggtccaccgc cacgccctct gccgcgctcg
     3181 tggaacctcc cggcgctgga tctgggatga acgccgcgac tttaccagtt ctggcgctcc
     3241 cgattcgcat gcctcttttg cacccggggt tgtaaccgta acccggctgg tctgttgact
     3301 ccgagtccac cacgtacatc gtgtcatcgc ttgcgataaa aattccgctt gggcggccaa
     3361 actgtttcca ttcattcagg aattttccgt cctgagtaaa aatctgaatg cggttgttgg
     3421 ctcggtccgc cacaaaaagt cgtcccttgg aatccagagc aagcgcatgc ggcgtattga
     3481 attcgcccgg acctgttccc ggttttcccc attccttaat aaattttccg tcttttgaaa
     3541 actttacgat gcgtgcgttc atctgcgaat tgggcggcga attgtgcccg tcagccacaa
     3601 aaatatcgcc attcggcgcc acaaggacag ccgacggacg gttgaaagtg tcatgggtag
     3661 cgccgggtgc gcctgctttc cccagcgtca tcagaacttt tccattagga ctgaacttga
     3721 aaacctgatg accctttcca tgtttgccaa cgtcgtcgct cgtgcctcct cctccgaccc
     3781 cgtcggtgac ccaaacgttt ccatccttat ccacaaatat tccgtgcgga agcacgaata
     3841 atcccgcgcc aaaatttttc agtattttac cggacgggtc aaattcaaga atgggaggca
     3901 cgttagaatc agcacagcta tttccgccgc atcgttcata ggtccagatg tttccgtcag
     3961 gaccgatggc cacacccgat gtcgctcccc attttcgccc atcgggtaat tttgcccagt
     4021 tggaaattgt tcgatacgga ttcggcaagt cgttcggggg agagtagctc gcctgggcat
     4081 gcgcgctccc actcacgatg gaaagcagta gcaggctcaa ggccagcgaa gaaaaaaaac
     4141 gaggcacttc tctcatgatg aattcccttt tcttagtgtt taagagaaaa aagatcgccc
     4201 gcccaaaggt cgggcggcct ctacgaaacc aaatttagaa ccggttttcg tagaggcctt
     4261 tccgtcgcac gttttttgcg acgggcaggg cggccttctt cgccagctaa atggctcccc
     4321 acaactctcc ctacttgctc tcaatattta cggcgtagta gccgaccgtt tttgtagaaa
     4381 gctctttcca ccagtgcggc gtttttgcgg gaatcgtgat cacgtcacca gcggtcaggt
     4441 gaaatgtttg tccaccttca atggacgtgg cagtcatctg gtccggactg gtccgcttta
     4501 ccccgatcat cgttccgccc gcaataaacg tagcctcacc atcaaccatg atgaaaatat
     4561 ggttggtgtg ctcatgatat tccaccgcac cggccgcccg tcgattggcg aggatcagaa
     4621 gtcccggatc attcaggatc ggtcctcctt tggccatgac cgtggcaacc tcatcatgag
     4681 ggatgaagtg aacagccgcc gggacacctt ctccggtaga ggctgcggaa gcggtgagca
     4741 agattgccat cgtaaataat gcagtcttta ggaattggaa tttcattttc gtcctcatca
     4801 tgccaaattt taaaagattg cccgcgattt cccaagtgca tgcacaggaa ttgttttact
     4861 gcgcggcaac cggccgcggc acatgctgag tcattaacac gagcggtccg tcagccggtt
     4921 tgaactgatg cggcgtgcct tcgggaataa tcgtgaaatc gcctttgcca atctgctgaa
     4981 ttgttccgcc gtcaatcgac gcgccagaaa ggttcgtcgc attcgtgcgc ttctctccaa
     5041 cgacagtgcc gccggtggtc aacgtgccgc cgccatcgat cacatacacg atttcagctt
     5101 ccttttcgtg gatggctacg gcgccttcca caggccggta ttcaaggttc gtcgtgtaag
     5161 gggaaaggga aagaattggc tctgcgacca ttacctggcc ttctttgcgt tcgctctttg
     5221 ccttggcgag gagagcctgc acatccgcag cggacgaaaa agttttcatg ggtgctgcac
     5281 tcatctgagc tgcaggagct tgcggtactg ctgccggttg ccgagccatc actacaaccg
     5341 ctgcaatcag agccaacaca acgcttattc ccaacgttcg catctttcaa ctccccacat
     5401 caaatttccg ctttgattta cggatgggca atcttaatac acagggaggg ccttttgcgc
     5461 gaactcaaat cgaatttaaa tgaaagtgga aaagatgtac gatttcgcct cgccatcgta
     5521 ctattccact tgctgggact gtccggaacc gacactgaat ttttcgcgga gaccaaatgg
     5581 taaaactatt ggcacactgg atcctgagcg ctctctgtct gctgctggtc gcacacttcg
     5641 ttcctggatt ttatgtcagc ggatttgcca cggcgctgat tgcagcggtg atcatcggtt
     5701 tagtgaacgg aacaattggc atgctgctga aaattctcac gtttccactc accattctta
     5761 catttggaat attttggcta gtgattaacg cgctgatgct gaaattcgct gcgctctttg
     5821 tgcccggctt cgaggtgcgc ggcctgtggc cggcattttg gggtggcttg attctaagca
     5881 tactgaatat gatcgtccgg catttcctga agaagagtaa tgaataacca cgcggaattg
     5941 aatttaagtg gcatagggtt cagcatgtgg gtggaattgc gagatttaaa cctcctagag
     6001 gttagtcctt gcagggtcta aaccccgcag gctgaaaccg gcgccgctgg tttcagtaat
     6061 cttcgacagg cttgcggcta aatggagact tcggcgttcg aggcgcgcgc gcgatgcggt
     6121 aatcttccaa ctccgcgttc gtgattgcgt agtgcgtttc tgggggatgg ttggcgtttg
     6181 cttccagttc gcggcgtagc ttgcgggagc aaacatccgg cgcatcctga atgcgcagca
     6241 ggcgttcatt gaatgccgct gctggaatgg tcagagtgaa ttcgcgaggc tcggtcgaaa
     6301 cttcgcgcac ggcaaatttg tactcacgct caagcggctt tacttggtaa cccaaaaatt
     6361 gaacgatgat ttcgtggctc acttggcgcc tgcggctaag ccaataaccg gagtctctgc
     6421 agtcggggcc actgctgctg ttttcttggt cttcttcgtg cgccgccgtc ttggcggtgt
     6481 gccctccgca agatcgacga ccatcaggct cgtgacaaac tttttcatgc cgtgaacgtc
     6541 aaagtcaatc gatgtgcggt ctgaattcga ttcggtgaca acacccaaac cgtattgaaa
     6601 gtgcttgatg tatgcgcctt cttcgagagc tttcaagatg cctcctcgcg tgaaacgtgc
     6661 cctggcgtaa cctttcacgg tcgccattct gagggaatgg gccaaaaaaa tagcccgcaa
     6721 gtgtggactt gcaggcctcc aagaatctaa aggtttacaa gagcaacagt ttcaaatcca
     6781 ttttagcacg ttgaattggc gcaacctaac gatatccggc ataaaaccgc ggggaattag
     6841 agacaaacca ccgtcatcgg gctggcgaaa aaatccaatc gaaggggaga attcgcaaaa
     6901 gcctcgtctt tgaggctttg cctgtccacc cgcatctcgg gctttcgcag gtgatctctt
     6961 cctcgccgaa gattttcatc gcctcgtttt cagagagcgc cggctcggaa cattttcgtt
     7021 ggaaattgat attatgcccg cctttggggc atgcgaacac gattcggtat gcgttgctca
     7081 tacctcagga taacacttat tgttgattct ggcgaatcgg aggacttgca tcatgcaaat
     7141 cggcgctgtt tgtgcggtga tctgaatgct ttctgaagaa aggtgaggca atatcttcat
     7201 cgcctcacct ttgaaatgca gtgccctagt gttttgccgg atagtctttt tggaaaccgt
     7261 tacggctgtt ttttacgcta aagctgccat caggctgcgc ggtcaattgg atgtaattca
     7321 cggggccttc attcggaacg ccggtttggg ccgttccgcc cgttccttga ttggcaatga
     7381 atttctcagg catgttgtgc cccccggcgc cttccgcgaa gtgcaattgc cagaggtctt
     7441 cgagtcccgg tgacgacttc aacgtttcga acgtggccac ggctccgccc ttattcgctc
     7501 cgttgtccat caccgcaacg cggggccgca tcgcatgcac cagcatcggc agactggaga
     7561 tgtcctgtcc atgatgcgag acaacatacg catcaatcgt gccgatgagg ttcttcgggc
     7621 aggccaaatc gtgttcctta ttccaggtca gatcgccaaa atcggcgatg cggaatttgc
     7681 caagagaaat caccatgcca acggattgct tgttctcgcc gccgactaga gtttctttca
     7741 tttcgtcctt gggaacaaat ccgccgcaat cgggatttgc ggctcccgcg ccgggcagcg
     7801 gtttggaaat ggtttcagcc gccgcggaaa ttacctgaac gtcgagccct acaatcggga
     7861 ccttgtctcc gggtttgacc tgaatgtgct tgccttctcc acggaccgta agataagcgg
     7921 cgtaagtttg cggaacattc aacgtcgcct ctgcgcttgg cccgtgatcg acaaaattct
     7981 ttatcgggat ggcccgggcg agatcaacca ctccgccagc atggtctccg tggtagtgag
     8041 tgaggaccag ataatcgatc tggctaacgc cggccatttt cgccacgctg acaatgcgtc
     8101 cagaatcgcg cgcgcccgcc caacctgtgt cgacgagtag tgattggccg ctgggcgaaa
     8161 cgaacagggt cgattgcccg ccttcgacat cgataaaata agcttccaga gcctttttct
     8221 gttgggcttt cagtgcgggc gacaacaaac cgatcgctat taccattgca agcaaaagag
     8281 tcttgcgcat gattcctccg acagttttga atataacggg aatgaggggc ccatttgttg
     8341 atggccgccc ctcagggata tataaaatca cccgctctaa ggttgcggca cagacagcgg
     8401 tagaaagtat acacaattaa aatcccgcgg aatgcgaatt ttgcttgtca gcagcacgtc
     8461 ccagcgatta cgcttttggt tcatgtcccg caaccttaca cggtctcgca ttgtggctac
     8521 ggctgcgcgt ttggcgctgc tgttcttcgc cgtggcgatt tgcgcggctg cccacgatat
     8581 tcccagcgat gcgacggttc agatgttttt caagccggat ggtaaccatc tcaatatttt
     8641 gatgcgcgtg ccgttgaaaa caatgcgcga cgtggaattt ccggagcaag gccagggtta
     8701 cctcgatttt gaacgtgttg atccggcgct ccacgaggct gccacgctgt ggctctctga
     8761 ttttatcgac gtgtacgaag gcgatacgcg cctgccaaag ccccgcgtgg tcgagacgcg
     8821 cctttcgctg gagtccgatc cttcgtttgc ttcctacgag caggcgctcg cgcacctaag
     8881 cgatcctagg ctcacgaaca aaacgcccat tttctgggac cagttgatga tggacgtttt
     8941 gttcgaatat cccattcagt ccgaccaatc gcatttctcg attcatcctg ggctcgatcg
     9001 tctggcctcg cgaatcgtta tcgctctacg ttttctgcct ccgggcggcg cggtccgcgc
     9061 ctttgaattt ctcggcgacc ccggactcgt tcatctcgac cctcgctggt tccaggccgc
     9121 gggccagttt gtaaagcttg ggttctttca catcctcgat gggaccgacc atctgctgtt
     9181 cctattctgc ctggtcatcc cgttccggcg atttcgcgcg ctgattccgg tcgtaacctc
     9241 atttactatc gcgcattcga tcacgctgat cgcttctgcc tataatctcg cgccggacgc
     9301 gctgtggttt ccaccgctga ttgagacgct catcgctatg tccatcgtat acatggcgct
     9361 agaaaacatt gtgggcggcg caacggtcca gcgccgctgg atgatcactt tcggcttcgg
     9421 cctcgttcac ggtttcggct tctcattcgc gttgcggcag acgatgcagt ttgcgggttc
     9481 gcatttactc gcttcgttgc tttccttcaa cgtcggcgtg gaactggggc agcttcttgt
     9541 gctcgcgcta atgattcctg cgctggaact tctattccgc cgagtggtgg ctgaaagaat
     9601 gggtacgatc atcctttccg caatcgtcgc gcacacggcc tggcactgga tgctggagcg
     9661 ctaccaaatc ctgagccagt acccaattca cgtgccaacg ctggaggccg ctccgctggc
     9721 gaaggcattg cgcctcatga tgcttatcgt catcgctgca ggtttggtgt gggtcgtctt
     9781 cggtctcctg tggccccgca tcggcaaagt agagcaagtt gaggaagacc ccgccgtgcg
     9841 tttgccataa ttttttgtaa agaaacttac gcctgccttt gaacgttctc catggcttga
     9901 catcggactg aacccgttta cactgggcgg gtcccaaacc attcccgtgc gcgctgtgaa
     9961 ttagcgatta caagatatgc gacaaaacac accattcagg acaattgtgg ctcgcatcct
    10021 cagggtgaac ggccgatgat cgatgtccgc gaagaaatcg ccaacgcacc tatcgggcgc
    10081 taccaccgct ttctcgcatt gctcatcggc atggtcgtat tttttgatgg atatgacacg
    10141 ttcaatccag cctacgtgat tcactatgta gcgaagccgt ggcacttggc tccggggcag
    10201 gccggactac tcgtttccag cggattgatt ggattcatga ttggttcgct gttgcaggga
    10261 aaattttctg atcgttacgg tcgtcgaacc acgcttctcg tggcgctttg gattgttacg
    10321 cttttcagtt ttgcgaccgc gacgctaggg aattcgttct tcgctttttg tggcttgcga
    10381 ttgcttaccg ggctcggcat gggcgcgcta ttgccgctcg gcgtcaccta cgtaaatgag
    10441 tacgcgccgc gccgcctggc caataccttt tcgatgtggg gttgggcgct gggctgggcg
    10501 actggtggca ttgtcgccgc tgcggtcgga gtttatctca cgccgatctt cggttggcag
    10561 gcactgtatt actcagcatc gctctccatt ctattagtcg tcatctgcca cgtggcgcta
    10621 ccagagtcgc tgcaattttc cgcgatgcgc ggaaattggc ggggaattgt gcaagtactt
    10681 tcccgtttga atccttcgca ggcgtcgcgc tattctgtgc ctgacgaaaa atttatcttc
    10741 ccggagccga gcgaccgtcc cgcgtccatc tctttattgt tatcaccacg ctatcgacgc
    10801 acgactcttt ccgtctgggc ttctgccttt tttattctgt ttggaatttg gggcctttcc
    10861 ggatgggttc caacggccat gatgcaacgg ggtgaaggat ttgcgacaag cttcagcttc
    10921 ggcgcattga ttcaggtcat ggcctttctt ggttccctta tctgcggata cattgcagac
    10981 cgcacgggca gggatcgcga ggccatgtcg gtatggtggt tcggcggagc gatttcagtc
    11041 ggcatcctcg ttctgtttaa tattcatgcg ttgaatataa tttgtgtagg ggccgccggt
    11101 ttctgcatcc tgggcgggca aaacattctg aataatttca ctgcggcatc ctatgatacg
    11161 gaagtgcgtg gcaccgccgt gggcatgatg ctgggagtgg gccgtgccgg tggaattctc
    11221 ggcccgttca tcaccggtct cctgcagcaa cgtacgcccg gcacgtccgg cctgttcgtg
    11281 gcgatcggag ctgcatcgtt catcggaggg tgcgccattc tatttgcaac gtcgagaata
    11341 tcgcgtgggc aaattgcaac ggcagaggta gtgcgcacct gagcaggcga gaagcgagtc
    11401 tcgccaggta aatttactag cacgaacaga aaatccccgc gtcggaagac ccgggcgaca
    11461 gcaatcgtga aaaccatgcc gcattctgta gcacgcctct ccagagagag gcctttctga
    11521 tagttacacg aaaaaagttc gacaatgcag caccgttgta acttaacgac ccggagacgc
    11581 accaatgacg cagtcaaagc cagccgctct aatcacaggc gcagccacgg gaatcggccg
    11641 cgccacagcc gttgcgctgg caggagccgg ttacgacgtt gcaatcaact acagccgcag
    11701 cgaagccgca gcgcgtgaaa ccgccgcgca agcgcaagcc aaaggcgcga agacactgct
    11761 ctttcaatgc gacgtaagcg acgacgcatc cgtccgaaaa atgcttgcta ccgtcgagaa
    11821 agaattcgga cgcctcgacg cgttggcgaa taacgccggc accacgtcga acgtcaagcc
    11881 cggcgatttc gagggcatga ccgctgagga atgggaccgc gtattcgcgg tcaatgttcg
    11941 cggaatattt caagtcacgc gcgcggcagc tccgatgctc aaggccgcgc gcggaagtgt
    12001 ggtcaacacg gccagcatcg tcggccttcg tcctggaccg cagccgctgc cctacgccgc
    12061 aagcaaagca gcagtagtga gcctcacaaa attgctggcg ctgaatcttg cgcctgacgt
    12121 aagagtgaat gccgtagctc ccggatggat ggaaggcgac tggatgcagc gcatgctggg
    12181 tgaaaggtat gatgatttaa tggcgcgccg cgcgaagagc actccgctgc ggcgctgcgc
    12241 cacagccgaa gacgtggccg aagtcatcgt aaatttgata accagcaacc gcttcataaa
    12301 cggcgaaata atcgtaatcg acggaggatt ttccgctaca acgtaggtat ttctagcgcc
    12361 ggtgccacaa taggcgcact ccgacattga atattgcgcc acatgaaatt tccaagggag
    12421 gaagatccgt gctggaagga gtggttccat ttccgcctga atttgcaaag cgctaccgcg
    12481 aaaaaggcta ttggattgat aaaacgctgg cgcaggaata cagaatcgca ttcgataaat
    12541 acgaaaagcg catcgcgctg ctcgaccgcg atcgctcttt cacttacggt gacctcgatt
    12601 ggatgtcaga caaccttgcg ctgaatcttc tggaaatcgg ttttcgtcca ctggaccgcc
    12661 tcgtagtgca attgccgaac gtcgccgaat ttgttgttct gtatctcgcg ctgcagaaaa
    12721 tcggcggcat tctgattgcc gcgctcatgc cgcaccgctt cgcggaaatt agccaatttg
    12781 tggaattatc cggcgccgta gcttgcgttg tgccggacga gcagggtgat ttcgattact
    12841 gcgcgatggt cgaccgcatt cgccaaaaag tttcgcacct gcggtttgga atcgttctgg
    12901 gacctgcgca gcccgggttt ttctcactga gcgatttgat ctcgaagcct gcgacactgc
    12961 ctaaatccgc attggccgaa attcaactcg acccggaaga tccatgcatc tttcaacttt
    13021 ccggaggcac aactggtatt ccaaaactga ttccgcgcac gcacaatgac tacgcgtaca
    13081 acacgaagat ggcgtcaaaa gtttgcggcg tcagcgagga caccgtcctc ctcgtaattt
    13141 tgcccattgc gcacaatttg ccactcgctt gtcccggcat tcaaggcgtt ctctttaacg
    13201 ggggacgcgc ggtgctcagc acgagcacgc gcccggaaaa tgtttgcgct ttaatcgaga
    13261 agcaccgcgt cacgcacatt aaagtggttc ccgccttgct catccgttta ttgcacgatc
    13321 cggcggtgac tcgccatgat ctttcttcgc tgaaaattat tcagagcggc ggacaatcca
    13381 tgctgcccga agtgcgcctt ctctcaaaaa aactgattcc gagcgccttc gtgcaggaaa
    13441 atttcggaat gtccgagggc cttctgatgt ttgtgcggtt ggacgatcct gaggatgtga
    13501 gtcaggaaac agtaggccgt cctgtatcgc cggatgatga agtgcggctc cttgctgatg
    13561 acgaccgtga aatgctgggc ggggaagtgg gcgaactttg ttgtcgcggc ccgtatacgc
    13621 tgcgaggtta ttttggcgtg ccggaatata atgcgcgaac ttttacctcg gatgggtttt
    13681 atcgttccgg cgacctgatg cgacggcaca aatccggcaa ctacattgtc gaaggacgca
    13741 aaaaagattt gataaatcgg ggaggcgaaa aaatcagcgc cgaagaaata gaaaatctga
    13801 ttctctcgca ttcgtcggtt aaaaatgcat cctgtgtacc gattcccgat cctattctgg
    13861 gcgaacgaat gtgcgcgtgc gtcctgttgc gagaaaacac acaactgaat ttcgacgagc
    13921 tgaaacaatt tttgctggcc aaggaaatcg caaaatataa attgccggag cgattggaag
    13981 tcatgcagga ttttccgctg tccccgtttg gtaaagtttc caaaaaatcg ctgaccgaaa
    14041 tgatcacgca aaagatggtt gaggaacaaa aaagtaaggg cggtgcgtca gcgtagtcgg
    14101 ggaatatttg gcgcaggcgc aaaaatcccc gggtctggag acccgggttg cagcagttgc
    14161 atagacgata gtgactgctg tagcgagcct cgttagaggc gaggtttttc gtaaagctac
    14221 ggaactctgt ccagaggaaa actgtgagaa tcatcgacgt ccactgctac ccaaacacgc
    14281 gcgaatggat cgcgtgtcag caaccttatg ttgacgcgct ggcgaaatac tggaaccgcc
    14341 catgggttcc aaaagaagag gacgaagtcg ttcaggaatt tacgcaggca ggcgtcgaag
    14401 cgattcttgt agcgctcgat ctggagacca caatcggcac gccgccttgt tcgaacgact
    14461 acgtctcagc gatccagaag cgccaccctg atcgaatcat ccaatcctgg gcggcggttg
    14521 atcccttcaa aggtgatgac gccctgcgcg aggcgcgccg cgctattgaa gatctgggaa
    14581 tgctgggctt tcatttccat ccggtcatgg gccattttgc ggtaaatgat ggccggctct
    14641 acccgttgtt cgaactgatc gatgcgctga aagtgccggt gatgattgac gtcggtacca
    14701 ccggcatggg cgcgggcatg ccgggcggca tgggcgccaa aattcgccac gcacatccgt
    14761 ccgcgataga tgaactcgcg gcggattttc cttcgctgac gattctggct gcgcatcccg
    14821 gttggccctg ggtggatgaa atgaccgcgg tggccctaca caagggcaac gtattttggg
    14881 aattatccgg atgggcgccg caatatttcc cgccccaact gaaaaaagat attcgcggac
    14941 gcctgaaaga taaaattatt ttcggaagcg actatcccag cattccctac gagcgagtgc
    15001 tgcgagaatg ggccgaactc ggctacacga gcgaaattat ggagcaggtt ttccacggta
    15061 acgctgagcg tgtcctgaaa gtttgaattc cgcgccatcg gcaataagga attccggttc
    15121 ctgttgcgca cgtcttcagg cgggcacttg atcgaacccg cgaccaagat tcgtcgcgtc
    15181 atcactccgg ttcacatacg cctttcctgc gcgaatcgcg gccgtgcttt gcagtttctg
    15241 cagccccgta cgcttcttgc cagtgctgtc cgtaaatgta aatgacccat cgcgagcttc
    15301 gaggatggtg atatccgcca ccgtgccggg acgtaaagtg ccaaggtcgg cgccaaaatt
    15361 aaacatatgc gtgggcttta gcgtgacgcg ttcaatgact tgatcgatgg tcatacctat
    15421 cgctaaaaat ttggaaagct cagtgaccag gtcatagacg ggcccgttga tactcaaacc
    15481 gccgaggtcc gtgccgatgg tgtccggtag aaaattttgc tgcatgcatt tttccgccac
    15541 atcaaggctc aggtgagggc cgccatcgcc tacatcgaac aacacgccgc gcgcgcggca
    15601 ttcaagcatt tcaggaagga tttttccgtt cgcatccagc ggtccgttcg ggcgtcctgt
    15661 gtagcagtgc gtcaggatgt cccctttgcg tagttgcttg agtatctctg gtagagggga
    15721 atacgaatcg ccgacatgca ccatcagcgg aagatgcgaa atttccgcag cctgcagcgc
    15781 ccgcttcaaa acttccagat cgtttgctcc ggtgatttcc ctggaaagcc gcgctttgat
    15841 ggccacgaca tccggtttat tggcgtcggc agcgcgagcc gttaattgcg gattgaccca
    15901 gtcgagattg gagtaatttc ctgccaaacc catgagtgtt ccgcgtgcgc cgatatcaat
    15961 catcgcgtaa acccgggtgg cggaaggttt gatgacgtat tgccggaatc cagcaatcga
    16021 aaaatatcct gcggagcctg cgtcgaccgc tgtggtgacg ccgcggccca agcaatattg
    16081 atccgcattc actcccgtgg gcccaattcc ttcaaaaaga tgcgcgtgaa cgtcgatcag
    16141 tcccggcgtg acgattctgc ccttggcgga aatcaaagtt cgcgttccat ccactggaat
    16201 gtctggcgcg acctgcaaaa ttttgccgtc cttcaccgcc acgtcgagtt gtgcatgcaa
    16261 attttgactg gggtcgatga ctgtgccgct cttgatcagc aaatcgcggc tctgcgtcag
    16321 ggccgcgccc atatcttgtg gcgatggaac ggcagcgctg gtcgcgcggc tgaaactcgg
    16381 tgcggcaagc agcggcgatc ccgcaagtaa ttttaaaaac ggtcgacgtg tgagcatgga
    16441 actcctcttt ggtcgttcat tgtcacttaa atttaaagcg tcacttttca ccagcgatca
    16501 accgcaatca ctgcggctct gatgttgcct atgtgagcga agctatttct tttcgtatcg
    16561 cttgaaattt cgtggaccta cttccgctcc gtatacattg cccgcatcat ccgccgctac
    16621 gccttcagcg gcagtcccat tcgttggctc caatccttgc ccaggattta acactggatc
    16681 gggaataaac gccgtgacct tgccgttttt cacgcttccg actcggatac cacgccggac
    16741 gccgggatta cgcgacatgt tcgattccga atcagcgacg tacaaaacat catgcttatc
    16801 gatgtaaact ccgctaggcc tgccaaattg tttccactct tcgaggaatt ttccatcctg
    16861 atcgaatatc tgcacgcggt tgttccctcg atctgcaacg aacaatcgac ctttggaatc
    16921 catcgcgagt gagtgcggat tattgaattc tcccggttct gtcccttgtt ttccccatga
    16981 tttaataaat tttccgtccg gagagaattt cacgatgcga gcattcgagc ccggcccatg
    17041 cccgtcggca acgaaaatgt cgccattcgg agcgatcagc acatcgcttg gtttgttgaa
    17101 tgtgtccggc ccatcaccgg cgattcccgc tttgcccagc gccaacaata cttttccgtc
    17161 ctgattgaac tcaaatacct ggtcgccctt gccgttttcg gcgtcgccgt ccgtcaccca
    17221 gatattattt ttcttatcga tgaagagatc gtggggccgc acaatcattc cgacgccaaa
    17281 acttttcacc agatttccag acgcgtcgaa tttcagaaca ggcgcgagat ttgagttggc
    17341 gcaagaagtt ccgccgcatc gctcgacgac ccagatactt ttttcgtcgc ggtcgatgtc
    17401 cgcgctgatt acctgtcccc acgttctgcc ttcggggagc cttgcccagt tttcaacggt
    17461 gcggtaagga ttgggcgcgg aattgagatc ggcttgtgca aacgcgctca cgccgcggat
    17521 tccaattaac gccgcgcaca gaatccccaa cgcgacaacc atgcgattca cgagtgacat
    17581 gcacccctca ctaactgtca ttttgaaaaa aagtattttg cttcgcgccc atcttacttc
    17641 acttgcgcac ctttcttcca accaccgcac atcaggcagt tgacttcttc ctactccctt
    17701 cgatcttggc aagacgtgat ataagattcg tcaacgaaat aacatggcaa cggaaggaca
    17761 tgcttatgtc acagatcatc gaaccgaaaa cgactgatcg caaatggctg ggcctctatg
    17821 accgccctca tggcgatctg cgcgaatttc tggaacgcgc cgaaagcgtc ggcgaagtca
    17881 cgcacgtgaa gggcgccaac tgggacctgg aacttggcgc gcttgcggag atcgtcaatc
    17941 acaaaaaatc ggaagctcct gcattgctct ttgacgacat cgccggttac ccaaaaggat
    18001 ttcgcgtcct ttccggctcg ggaaactcgg caaaacgaat ggcacttatg ctcggttttc
    18061 ctacgccgcg gacagccatt gatgtcgtgc aggctttccg cgatcgcatg aagcagcaca
    18121 agccgatccc gcccgtcatt gtgaagcaag gcccgatcct ggaaaatatc gatcgcgatg
    18181 atgacgtcga tctctacaaa tttcccgcgc cgcgcgtgca tgaaagagat ggtggccgct
    18241 acatcggcac ggaagatctg gtaatcatgc gcgatccgga agaaggctgg gtgaacgcgg
    18301 caacgtaccg cgtgcaggtt cactccaaaa attcgacggg tttgtggatg tctccgggca
    18361 aacatggcag gcaaattcgc gacaaatatt tcaagcaagg aaagccttgc cccgtgctgg
    18421 tgtgctgtgg ccatgatcct ttgttgtttc tggctgccgc gaacgaaata aaatttggcg
    18481 tatcggaatt cgattatgcc ggcggacatc gcggcaagcc attcgaggta attcacagcg
    18541 aaattcacgg gttgccgatg cccgcgcacg cggaaatcat tttagagggg gaattaacgg
    18601 aaggtgatgt tcacaccgaa gggccctttg gagaatttac gggctattac gcgagcccat
    18661 cgagtgagca gcccgtgatt cgtgtgcgcc gcgtctacca tcgcaacgat cctattctga
    18721 cgatggctac gcccatgcgt ccgccgtcgg acatgtcttt cggcaggtcc atcgtaaagt
    18781 ccggaatgat ttgggatgcg atcgagcgcg cgggaatatc cggagtgcaa ggcgtgtggt
    18841 gccacgaagc tggcgcgatg cggttgttca acgtaatttc cgtaaagcaa gcttatgcag
    18901 ggcacgcaaa gcagatcggc cttgtggccg ccggttgcca ggccggtgcg tatcttgggc
    18961 gtttcgtcgt tgtggtggat gaggatgttg atcccacgga tttgttcgat gttgtctggg
    19021 caatctccac gcgctgcgat cccgcactgg atattgaaat tattaagcgc atgtggagcg
    19081 gtccgctcga tccgattatt ccgcatggtt cgcccgtaaa ttacagttcg cgcggactga
    19141 ttgatgcgtg ccgcccattt gagaggctta aggattttcc gccggtcgca ggtgcgacgg
    19201 cagagttgaa agccagagtc gcggcaaaat ttgcggacat actgaataga attgtctgaa
    19261 atattgtaag tggtgcacac ttctgcctgt ggaattttgt aaggtctaaa ttccgcagag
    19321 tctgtgtgag aactgcagaa tctttgtggg tcgtggcttt agccacgaca taagtcctgc
    19381 aaaatgagtg cggctttagc cgctgaagct tcaattttgc agttctcaca cagactctga
    19441 agcccgcgcc actggccgac ccatgacgaa tgcgaaaact attaacgttc cagaattaat
    19501 cgatgcgcaa aatatcggcg cgtttcagat tcgcattatg atcctgtgcg cgctggctgc
    19561 gatgctcgat ggttttgccg cccagatgat cggctatatc gcgccgtccc tggcacatga
    19621 catgcatctg gcacccgcca cattgtcacg aatttttgcc gtgagcttaa ttggtctaat
    19681 gcttggcgcg ctcactttcg ggccgattgc cgaccgcttc ggccgcaggc gcgtgatcgt
    19741 catatgcacc cttctcttcg cacttttcac tctggccaca gccttcacgc attcggtaaa
    19801 cggcttgatc gctctccggt ttttggcagg cttgggattt ggcggagtaa tgcccaacac
    19861 aattgcgttg accgcggaat attctccgca acgccgccgc ggcacgatga tcaccatcat
    19921 gttttgcggt tttccgattg gcgcaacgat tgtgggcttt gccgctgtgc ccatccttcc
    19981 tgcctatgga tggcgaggcg tttttgttct ggcaggtgta atgcctttgc ttttggtccc
    20041 ggtgctcgcg ctgctgctcc ctgaatccat tcggcatctg gtgattcacg ggaaggaaaa
    20101 ttcgcaggtg agagagttgc tcatgtgcgt gaatccccat ctcgtttttc cgagcgatgc
    20161 aaattttgcg gtgcatgaag agcgcgcccc aggcctgcct gtaatgcatc tgtttcgaca
    20221 cggccgtgca ctcgctacaa tttttatctg gatcgcattt ttcgttagct tgctcgacat
    20281 ctatttgctg tcgagctggc tgcccacggt gtttcacaac gcggggatta cactttcact
    20341 ttcagtcgtc gccacggcgg tatttcaagg aggaggtgta gtctccagtc tcatactcgg
    20401 catttttatc gaccgcttcg gagcgtttcg cactgttgct ttcatctacc tgcttggcgc
    20461 catttttgtc gctctgctgg gccactcgca ttccataggg ttcatcctgg cagccgcgtt
    20521 ttttgcaggc gcgggaatcg taggtgggca gactggcaca aatgtcctgg ctgcttcgct
    20581 ctatccaact tatattcgtt caacaggtgt aggatggggg ctcggcatcg gccgcatcgg
    20641 ttcgattctc ggcccaatct tcggcggcat catgctttcg cttcattttc ccctgaccac
    20701 aatttttctg gttgccgcaa tctcggcatt cataggcggc gcggcaatct tcttaatggg
    20761 ccgcagccag ccatctgcga aggccaaaga tattctcagt gttgccacct catgaatttc
    20821 aaagcgtttt atcgaatctg aatcttcctg ctactgcgtt tcttattcct gtgatatacg
    20881 gaaatgcagg atgtggtgca agaaaatcat ggcgctccaa gagtcaataa atcgaggttt
    20941 ctcatgaaag ttggcattgt tggtgcagga ggcgtcggct ccgctagtct tttgtccctt
    21001 gtcatgcggg gaattgcttg ccaagtcgtt gtcttggaca aaaatcatga acgcgccaaa
    21061 ggagtcgtgg ctgacctcca atacggcgcg acactttctc cggctgttga attgctcgcc
    21121 ggcgattatc ccgatcttgc cggagcggcg ctcgtcatca tcacagccgg ctccaatgaa
    21181 aaagcgggag gagccacgga ccgcaacgat cccgcaggca gactaaaact tcttgagacg
    21241 aacgcggcgg tgtaccgcga agttgttccc agcatcgttg cggtagcacc ggaggcgttg
    21301 atacttgtgg ttacagatcc accggatccc ttagctgacc ttgcccgcca acttgctggc
    21361 catgatcgcg tgttgagttc cggcactttt ctggactccc tgcgcttccg tttacacctg
    21421 gcgcgtaggc tcaaggtgaa ccccatgtat gtcgaggctc aagtggtagg agaacatggg
    21481 acgtcccaag tgtttttgtg gtcctctgca agtgtcgggg ggacacccat ttcgaaattg
    21541 cttgagaaga gcggccagtc actcgctgag tttcaacgga gcgtagagca agatgtccgc
    21601 tacgccaaca ttgcaattat tgaaggaaca ggcgcgagcc agcttggaat tggtatgatc
    21661 acggcgcgta tcgcggacgc gatattgcgt gacgagcgcg cggttatccc cattggctcg
    21721 tataattcca aatacgcagt cactatttca cttccaagcg tggttggccg ccggggcgtt
    21781 tatcaaatcc tggagccaga gatgtctgcc gatgaatcgc agtcacttca gcgcagtgct
    21841 gaagtgttgc ggcttgctcg gtcgcgaatt ctgccggcag ctccgcaaag ttccgtccgc
    21901 catgctggct aacgctttcc ctttgttttt aatctagacg tggtcattag ttggtggcag
    21961 gcgcgagatt ttgcgtcagc tcaatacgcg tgccccaagg atcagttaaa tacacaactt
    22021 tcgtagtccc attcggggca gtgcgaatcg gctcgtccag cttggttcct tgcgcgtcga
    22081 gttttttcac aaatgcttcg aggtcgatca catcgaaacc gatgtgatcg agcgaacggc
    22141 cttttgtagg agccagcgcc gtttttcctt gcgagaaaga aagattcacg cccgggagat
    22201 cgtcacaatc aatccatccc ggcgtcgcca ctcgcgcgcg cttgccggga attccgccaa
    22261 acgttttcgc gtaccatgct tgcatcgcgg gaatatcgag cggatagaaa tgaatatgat
    22321 tcatctccac cgcatgagaa atagaaggat caccgaagaa ttccacgcga atcccgtcag
    22381 gtccatgcac gtagccttga tttggatttg aagcctgctc gatctcaacg ccgtccgctt
    22441 tccatttcgc catcgtcgcc ggcaaatctt tcaacacaaa accgaagtga tccacgatcg
    22501 atccggacgg tggccccgaa gggtcggcct gccgcagcat aatgtaaact ccgggaaact
    22561 ggatcagcat gagctgcccg ttttgcacaa ccgtgcctcc cattgtcgcc gtccagaaat
    22621 gtttctgcgc ctcgacatct ttcacaaaca ggtgaatgtg tcccatggtg acgcccgcgc
    22681 tgttcggcgg catgagttgc gcgcgtgcgg gccgagccgc aaaggcggcg gcaaccagca
    22741 ggatcaagaa ctgaatcttc gtaaaagttt ttttcatttg gttttccgtt tcgtgtgcgt
    22801 agcgatctgg caacatactc cggtctgaat ttccaacaag caaacgaaat cggttttagt
    22861 agcgcgggct tcagcctccg gaattttgtt cttgcaagga ctaaactccg caggctgaag
    22921 cccgcgctag caagacctat ggatgcggcg cgacgttcag cctgttcagc gcgactcttc
    22981 cgttgttcag aaccatcact acgtcgccga tatgccgaat gttttcgagc gggctacggt
    23041 cgatcaccac aaaatcagct tcgtagccaa tgcgaatcga accggtccgt ttatcgaccc
    23101 ctaagtcctc ggccgacacc gaagtcccgg ccttgattgc atccatcgga cccagccctg
    23161 cctcagacaa ttcaataatt tcatccgcca tcgttcgatt gtttttatca aagtaggatg
    23221 tatccgtgcc cgctacaatt ttcaggccca tcttccaggc cttcgctgtc atctctcgtc
    23281 ctgtgggcag catggctttg ccgcgcattt ccaaaattgg attgtcatac tcgccttcag
    23341 gttcggacat atccacgagc gcggtgatcg tgggatcgag ataaacgcct ttttctttca
    23401 ttagccgcat cgtgccttcg ctcaaatacg tgccgtgctc gatggcgtgt acgcctgcgc
    23461 gtaccgcggc cgccgcgcct tcatcgccat gcgcgtgcgc gacgacccac aatcctgtct
    23521 tatttgcttc ctcgacaacg gcgctcaatt cctcatcggt gagcactcgg attcgcggat
    23581 cggtgtccgg cgttcccgcg cgctccgtgg ccatcacttt tatgcggttc acaccgcggc
    23641 tcaccattgc gcgcaccatt cgccgcgcag attccgctcc atgcatgccg ctcatcagat
    23701 cggtgtcctg cggaaaatca atgaagtaat cgtccgcggg atgcgtttga atatgatatc
    23761 ccgcggcgat aatatccggc acgtcggcca cgcccttatg attcagttcg cgcatgccga
    23821 cgtccgcaaa atggtttaag ccagcttcgc caatcgtcgt ggctccggaa cgcagcgctc
    23881 gcttggcgtc cgccaaattc ccgacgtgta cgtgcgcatc cacgaagccg ggaagcaacc
    23941 agtgccccga aagatcaatg acagggcctc cgccggcgga tgccgctccg tggccgatac
    24001 tctgaatgtg cccgttcaca acaactacgg tggcgtccat catgggcgca ttggaaattc
    24061 cgtcaatcat attggcgtga attaagacca tcgcatcgtt cgcaggagtt tgcgcgcagc
    24121 agggaattgc agcaaatgcg aggaaagcca tgactgccag cgtggcgtac gccaatcgat
    24181 gccagaagat tttcatcaat gtaatcctcc tagtgcgggt ttcgtagcgc cggcgtccag
    24241 ccggcagtct tgtgcactgg caccacatca taaaaatgcc ggcgctacaa aattcttccc
    24301 agcggcaagt gtggggaatg attcgatcgc cctactccgt ggcgatggtc ggaaccactg
    24361 cgtcagccgc aactttccat tggccttttt ctcttacaaa aacgagtaga gcgcggtgcg
    24421 gcgtgctcac gatgttgccg ctcgcatcgg cgatcttcct gattcttcga tccgtagcca
    24481 gggcgacatc accatacacg gacatcagct tgaattgatc cgcgaaaagt ccctcgccgg
    24541 gtttgaaatt gaggtgagcg acgcgctcca atgccgcggg tttggtcagg accacggtgg
    24601 gggcccatcc gataatctca acccacttgt cggcgtacat atctttcaaa tactcggtct
    24661 ttgcggtgcg cacggactcc atccactttt gatcgatggc tgagagttct ttttccagtt
    24721 tttcgtcggg cgtcttggtg gtcttggcgc ctgagttctt cgcgcttgct tccgcaggag
    24781 gaatgacggg aacgagcgcc gctgccgccg gacgccactc gccattctgt tttacgaata
    24841 cgcgcagaca atggctgtcc gctgtgaaag catatttccc atccgggcgc aaagtctgaa
    24901 aatcggtatg atccgttgca atggccacgt caccgtaaac ggctacaaat ttgagatccg
    24961 cgggaaaggg acgaacgcct gtggcaggat ttgccgcagc ctgcgtggca accatctctt
    25021 ctttattacg caccgtaccg ccggactgca cttcgaaaaa tccgggcacc caataattgc
    25081 tcctgaattt tacacactcc gcataaggtt tttgataagg accgtcgcat agccactgtt
    25141 gattcacatc ccacaatata ttttcaagat tgccttgctt cgcatcttgc gccgcagcct
    25201 tcccgggcaa gccgagcatc gccggaaccg ccagcaacag cgccgccacc catacgcgta
    25261 ccctggtcat cgcattcctc caccaaattt aggtgcaatg ctgcgtatca tagttcagtt
    25321 tcttgcgaag acgaagaggg aagagagagg gatcgcatcc gactgaattg gggaagggag
    25381 aaattcccgc ctgcaatgct tgctgcctct atgaaatcca acgggaatga atacgcactt
    25441 atccccaagt atcggcaggc aaattgacta cgattggttc cgacgtgctt cggacgacga
    25501 cccagcggaa tggcgtgtcc ttcgagcggt tgatttcgcg atgcacgagc catgcgggga
    25561 cgtgcagaaa atctccggcg tgcgcggtca acgaatgttc gccgcgctcg ccccactgaa
    25621 tgtacgactc tcctgcgagc acatacgcaa cggtttcctg caggccatga tgatgaatgc
    25681 ctgtctgtgc tccgggttcg acgagaaaag ttccgcccca gagcaaggat tggatgccca
    25741 aatcggtcga gatagccgcg agccgctgag agcctggcgt ttgagaagtt cccgcgctga
    25801 attcatcggg atgaacaacg cggatcgaag aagaattttt attcatgagc aatatgatgt
    25861 cgtgaaggac gagagtttgc aaactagcgg tgagacgaaa gtagaagtag cacaagctgt
    25921 gacagttagt ccttacaagg attaatcccc acagccttga agcctctgct actcaaccag
    25981 ccaatctaca tactaaattg tgctacatta cccctccaat taagttttta tttaattttc
    26041 cgggcgaggc cgcccaactt gcgacccggc cataaaattc agcggagctc taatgatcat
    26101 cgattgccac ggccactata cttccgaacc gccagccttt ctcgaattcc gcaagcagca
    26161 aatcgccgcg ttcagtaact cctccgcggc accggcgctg ccgcggatca gcgacgacca
    26221 gattcgcgaa aaactggaac ccgcgcaatt aaaatttcag cgcgaacgag gcactgacct
    26281 gacgattttt tctccgcgtg cctccggcat gggccatcac gtgggcgacg cgaaattaag
    26341 ctccgcatgg acgcgaattt gtaatgacat gattcaccgc atcgtcaatc tctaccccaa
    26401 aaatttcatt ggagtctgtc agctccccca atcgaccggc gtgccgcccg agaattgcat
    26461 cccggaactt gagcgctgcg tcaaggaact gggattcgtc ggctgcaatc tgaacctcga
    26521 tccatccggt ggctcgtgga aagaaccgcc gctcaccgac aagtggtggt atccgttcta
    26581 cgaaaaaatg gtggagctcg aagttcccgc catggtccac gtcaccgcga cgtgcaatcc
    26641 cgcttttcac acgacgggtt cgcattacat caacggtgat acgaccgcct tcatgcaatt
    26701 cctgacttcc gacctgttta aagattttcc gacgctgaaa ttcgttatcc ctcacggtgg
    26761 tggcgcagtg ccatatcact ggggccgcta tcgtggcatc gcacaagaca tgaagcgccc
    26821 accgcttacc gaactgctgc tcaagaacgt atactttgac acgtgcgtct accatcagcc
    26881 gggaatcgat tgcatgacca aagtgattcc ctcggacaat attctcttcg cgtcggaaat
    26941 ggtcggcgcc gtgaagggca tcgatcccga aacaggaaat tactttgacg atacgcggcg
    27001 ctacatcgac aactcaacga ttttgaacgc ctcggacaaa aagaaaattt atgaagcaaa
    27061 cgccttgaaa gtttatccac gcctcgcaag gcagatgcag aagcaaggaa tcggtgcaaa
    27121 ctgatagaaa tttaaccgtg cgttgaaaat cgtcacgggc aggacacttt ttcttgacga
    27181 accttggcgc ttttcgcaca atcaactcat ctacctggtt cctctgcgtg tcacacgaag
    27241 agcacatctt gagggccagc ataaacggaa atgcaacttc ggacaaaatg aggtaacggc
    27301 atggggaaaa tcgtattggc agccggagtc gtacacgcgc cacaactctt tgtcagacct
    27361 ccgagtgaag atctcgcgca actggacgcc gacattgcgg ctatgcgcct tattggcaag
    27421 gacctggacg agaccaggcc cgatgttgcg atcgtgatcg gcaacgatca catggaaact
    27481 ttttttctct cttccgttcc cactttcgcg atcatgtccg gcgaacgagc gcgcgccgaa
    27541 ttcgcgcgta aaacttacga tctcccggtc catcaggggc tcgcggaaga tttgctggac
    27601 aagctcgtga acgatgactt tgacatggcc tattcgcagg atgccgtcct cggccacgcc
    27661 tttgcggcga tgtatgagtg ggtgatcgag ggtagaaata ttcctgtcgt ccccatcttc
    27721 gtgaacgcct acctgccgcc gctgcccacg gcgcgacgtt gcgcacaact tggaaaagcg
    27781 attgccaagg tcgtcgcatc gcgccccgaa aaagtggcag tcatcgccag cggtggaatg
    27841 tctcactttc cgggcacatg gaaatatccc gcccctgatt ttgatttcga ctactgggct
    27901 atcgcgcaga tggaaaaggg caaccacgag gcgctgctga aactcacctc gcaacaactc
    27961 gatgttgtgg gaaacacgga aatgctttcg tggatgatta tgttcggaat gatcggcgat
    28021 caacccggcg aactcatcac atatcagccc acatggcacc acggccacgc ggtcatgcgc
    28081 tttcttcctg ataaaagaac tggaaaactt tcggaggaag ggaccaaggg acctgcaaac
    28141 tacacattca agggttcaga aggatacgaa ttttataagc atccctcgcc atcagcctac
    28201 aagctgaaca aaattctcta cgacctccgc ttcaagccga gcttgcgtgt ccgcctgatc
    28261 aacgacattc cgggagtcgc cgccgagtac ggcttgcctg ccgacgaagc caaagtcctg
    28321 gaaactttgc aggacgaaga tatcgagaaa ttccgcaacg gcaaggttca cccactcgtg
    28381 caagctggag cgcatccgct gggaatgtgg atgagcgtga ttgtgtttca cgcggaattg
    28441 cgcagactgc ggaccgtggc cgacaaaggc gatgcggaaa aagcaagcgc gacgcaataa
    28501 aaaatatgtg gcataggttt cagagcctgc gtttcaactg gagaatcatt gtgggtcgtg
    28561 gctttggcca cgacacaaat cctgcaaaaa tcagttcggc tttagccgct gaggttccaa
    28621 tgttgcagtt ctcacacaga ctctaaagcc tatcccacag gacaatcgat ccatctttcc
    28681 aaatagaatt catttcgctc cggaggaatc atgcccaaga tgcgcgtctt ccaagttcca
    28741 aaagccaagg acccttttca attagtcgag cgagaaattc cagagcccgc tacgggatcg
    28801 gtgcgcataa aagtccaggc ctgcggcatc tgccatagcg acgaatatgc caaagaggga
    28861 ttgcgcccag gcgtcactta cccgcgcgtt ccaggacacg aaattgccgg agtgattgac
    28921 gcgatcggct cgggagttca aggctgggag gttgggcagc gcgtcggagt gggttggcac
    28981 gggggacatt gcggttattg cgattcctgc cgtcgcggcg attttgtaac gtgcagcaaa
    29041 ggccggcaaa ttaccggcct cacatgcgac ggaggctata ccgagtacat ggtcatatcg
    29101 ggcggcgcgc tcgctgccat tcccgatgag atttccgcca cagacgctgg tcctttgctc
    29161 tgcgccggcg tcaccacatt taattctttg cgaaacagcg gcgcgcggcc gggcgacgtc
    29221 gttgccatct tgggtctcgg aggtctcggc catctgggcg tgcaatttgc atccaagatg
    29281 ggttttgaaa cgatcgccat agcacgagga aaagataaag aacaatttgc aaaaaagctc
    29341 ggtgccaaac attacctgga tagcgagtct caaaatgtgg ccaccgaatt ggaaaaactg
    29401 ggcggcgcca gggctatcct ctccacggta acgagcggga aggcagtgaa cgcagtttta
    29461 gacggcatgg gaatcagcgg gaaaacaatc cttgtgggca atcccgatca gccgctggaa
    29521 attaccgggc gcctcctgat agcaggtcgc cgttcgatca gcggttggcc ctccggctct
    29581 ccaatcgatt cgcaagacac gctcgaattt tgcgcgctca caggcgtgcg ccccatgacc
    29641 gaagtctacc cgctcgaacg cgcccctgaa gcgtacgacc gcatgctaag cggaaaagcc
    29701 cgcttccggg tagtcctgac cactggactt taagtagcac aggtttcaac ctgtggaatt
    29761 taggccttgc aacgtctaag ttccacaggc tgaagcctat gccacaaacg gcaaagttcc
    29821 actcactcac ttgatattgg atttcttatt ttgattcagg ctcttcgcgc ggatccaatt
    29881 taatgccgac cataaaaccg cccttgtcac ggcggcaatt ggtaacaatc cccgaatatt
    29941 gaagagtccc tgatttgatg gtgacgtgcg acccaaggct gattgaagtt ttcattcgca
    30001 gacttaagcc gccgcgagat cggtcttcca gtgtcgcggg tgcaacgcgc ggggatagtt
    30061 cattgtccat ccaaagaatg tcgacccgcg ccattagggg gatgcgctgc tctgctctgt
    30121 tcttttttcg ctcacgactt gcctatcggc aagaaacagc aaaacttcat cgcgctgatt
    30181 taagacgagt tctgcgaagt agaggtttac gaaacgtacc gttgtatact cccgcttatc
    30241 ttttatgtag tgacatatcc cggaagaaga acctatcgtt ggaaaaattt ggatcggaaa
    30301 cggggtttta tgagaattga tgtccattcg cattatttcc cggcggaata tctggatcga
    30361 ttggacctgt atggtggaag ccatgtcacc ggattaattc gcaagatgaa gatggattct
    30421 ccgggcttca gtggcctcga tgcgcacctg caaaatatgg accgctcaaa cgtggagatg
    30481 cagattcttt ccgtgtcggg acaactccct tattttgcga aggaaaatga tgcggtggac
    30541 gcggctcggc tcggcaacga tatctatgcg cgcattgtcc gcgaataccc gaaacggttt
    30601 gccgcgtttg cctgcactcc gctgccacac gtgcaagcat ctattgatga aacgcggcgc
    30661 gcactcgatg aactcggcat agtgggcgtg acggctggaa cttttgtgtt gggaaagacg
    30721 attgccgatc cagccttcga tttatttttc accgaactca atcgccgcaa agctgcactg
    30781 ttcatccatc ccacaggcgg tagtcttggc tcacagctga tcgagtccac aaacctagca
    30841 tggccggtgg gtgcgccgct cgaagacacc gtatgcctcc tgcaattcat gcaggctgat
    30901 ttcccgagcc gttttcccga cgtcaaaatt attattccgc atctgggcgg ctttgcacca
    30961 tttatcgcag cgcgccttga ccaattgcag gatcatttcc tgcctgcatc agcg
//
LOCUS       DASZMB010000182        21257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1590011, whole genome shotgun sequence.
ACCESSION   DASZMB010000182 DASZMB010000000
VERSION     DASZMB010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 21257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21257
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590011"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VG272_08340"
     CDS             complement(<1..228)
                     /locus_tag="VG272_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13727.1"
                     /translation="MRRLSRCVFFFAPAFFVICIAVSLNAQTGAKNGEWRTYGGDLAS
                     TRYSALDQVNAENFNKLAVAWRFKTDSLGPRP"
     gene            complement(700..1329)
                     /locus_tag="VG272_08345"
     CDS             complement(700..1329)
                     /locus_tag="VG272_08345"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF13728.1"
                     /translation="MSASEPKPLASTIVREDEALLVSAAKGGDITAFEELVNRYERKI
                     FRLAMNITQNREDAEDVMQDAFLKSYQNLDRFQGDSRFYTWLVRIAVNEALMKLRKRR
                     PNQVSLDEPLTDNDGEDSVFREIVDWGPSPEKKFAQTELNQILNNVIGELDPIFRVAF
                     LLRDEEGLSTDETAQILGISVAAVKSRLLRARLKLRQKLNKYFRRSETQ"
     gene            1676..2185
                     /locus_tag="VG272_08350"
     CDS             1676..2185
                     /locus_tag="VG272_08350"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HWF13729.1"
                     /translation="MRTLPDYLRSGMKLIIVGCNPGDRSARVGHYYAGRGNEFWPLLY
                     DSGVVPELLDHRTDRRMIEFGIGLTDLVKRPTRGVEELTREEFAEGRILLSQKLEQFA
                     PQVIAFNGAGTFENFAQRPCKPGLQKERLYGAQVFVLPWTGAKSENGETSKIRYFKQL
                     AKLLSELPD"
     gene            2351..2704
                     /locus_tag="VG272_08355"
     CDS             2351..2704
                     /locus_tag="VG272_08355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HWF13730.1"
                     /translation="MAKAKIKFLQKPSCTSCRKAKAFLEKQKVELDLRDLGKDRLSVE
                     ELDKLIGKRDHRTFLNTRNEMYRAKKMKENPPSREETLKLMAAEPNLIRRPVVLRGSD
                     IVLGYDEEALKRIAK"
     gene            2740..3297
                     /locus_tag="VG272_08360"
     CDS             2740..3297
                     /locus_tag="VG272_08360"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HWF13731.1"
                     /translation="MGIGEKIVLLQGDLTEMDVDAVVNAANNDLQLGGGVAGAIRRKG
                     GDQIQRECNEIGSIPIGGAAITTGGKLRARFVIHAASMQLGGETTAHALRSSTAHSLR
                     IAAEKGLRTIAFPAVGTGIAGFAIPECARIMLREVVEHLKKPTSLEKIYFVLFDDRVL
                     SEFEKARAEMAAAVEFDGAAQTEQK"
     gene            3294..3566
                     /locus_tag="VG272_08365"
     CDS             3294..3566
                     /locus_tag="VG272_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13732.1"
                     /translation="MKELTEKVLAELKQYEHPLFVFDARPAAEGVEILIHYSPPEPQV
                     HTYIFLMRPREIESKQFPWIFQKQLYDCLHDFIVEMFTCNPQRQDV"
     gene            3563..4741
                     /locus_tag="VG272_08370"
     CDS             3563..4741
                     /locus_tag="VG272_08370"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HWF13733.1"
                     /translation="MTIPPSFGAGAGIESAKPGEHSRALIEILARRIRAGGPITFAEF
                     MRECLYHPIHGYYSRASARCFGDYYTSVDVHPIFGRLLARQFAEMWKLLGSPRTFAVV
                     ESAAGVGRLAGHILDFSARELPEFYAALHYVAVERSSARRAHHAAQMGSHVTAGRAST
                     ADEIPGAISTGCIFSNELLDALPVHRVIMEHGSLREIFVEFENARFIEQREKLSMPAL
                     EEYFSEQGITLADEQQAEVCFEACDWIECAGRALERGFVLTIDYGHEAPILYNEHHNR
                     GTLLAYRAHTVTENILDAPGEQDLTSHVNFTAVDLWGRRVGLACTGLVTQSQFLVALG
                     RENEFADLYEPGQPEIEKLRARLLLKNLIHPEGLGEKFQVLIQHKGIATPQLTGLSGY
                     "
     gene            complement(4971..7187)
                     /locus_tag="VG272_08375"
     CDS             complement(4971..7187)
                     /locus_tag="VG272_08375"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HWF13734.1"
                     /translation="MSKFTILLVLASLASFALPSSAQSPGAPQGPSRGVAPVQTAPIA
                     AFRATNYEIRASLDTLGQVMNAQAKVDFAARDASRVVEVELNQNLRVNSIRDLSGKPV
                     NFDRDDMSAQKLRVTLNDTVPAGSQVTLFFDYGGPLSSRVLNPERGTRMAFIAKDGGY
                     LLLPSRWFPLTDFPSNRYTGIFQIEVPGNMTVVGTGILAGAPTSVTPKPAAAPVNSKP
                     ALAPSALGNSHSKSAAPVVPMNAPPPPPMENERMLYTYRVDKPEAAGTFVISPLQLNP
                     EHAQGMAFSIYTPPAAANTAPAYANSLAQVIDYFNDTFGPLPNPSMTVAQLPDGTVDG
                     YAAPNLLLVSARQWSVKPNERLLADLAAHQWWGSQVTAATPSDAWITDGLSRYAEGLY
                     VEQTSGKDALNKAIEDFAVGALMFDDSAPIADAGRLEDGTEEYQSVVVNKGAIVFHML
                     RAIIGDANFNALLKDFYTRYAGKSARIQDFEQLAESHMGKAAPAEFKMGNAPAASDEP
                     TSLRPFFTQWLHSTGVPEFNIEYVIYRTKKGFRIVGKAKQNLDVFHMNVEIEVQTEGN
                     PEFKTITISGKESPFTVETFGRPKPNGIILDPHDYILKSNQRLRVRGIIARGESLASQ
                     GRYYDAVIQYQKALEQDRLNALAEFRMGEAFFYQRNYAASAQSFRDALDGATDLTTKW
                     TEVWSHIYLGRIYDIQGDRTRAVNEYNKAKQTADDTGGAQAEAEKSMKKAYSEAGS"
     gene            complement(7427..8560)
                     /gene="lpxB"
                     /locus_tag="VG272_08380"
     CDS             complement(7427..8560)
                     /gene="lpxB"
                     /locus_tag="VG272_08380"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HWF13735.1"
                     /translation="MPPTQILISAGEASGDMYAARLAAALQSRADVHLFGMGGMRMRE
                     AGVEIVADSSEVSVVGIVEIAKKYPALKRVWNRLVGESSRRKPRFAILTDFPGFHLRM
                     ARALKRQGVQCIYFVCPQFWAWRPWRANLVRRRFVRGLCIFPFEEQWYRSRGVKADFI
                     GHPLVGNVSAKRTRAEFAAAYGLDASKPIVTILPGSRSGEIAHHMPTLMQACARIQKE
                     REVQFVLALAPGMKQPQVAQYLSAEVPVNVVEGATYDALGAADLAIVSSGTATVEAAL
                     MDAPMIVVYRLSPLTAGIARLLVRTPMFAMVNLIAGKRVVPELVQKEFTPDRVFGEAI
                     RLLDSAGARAEMRLGLAEIREKLGPPGAIERAANLIVSMLNQP"
     gene            complement(8723..10600)
                     /locus_tag="VG272_08385"
     CDS             complement(8723..10600)
                     /locus_tag="VG272_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF13736.1"
                     /translation="MSDFREEEALGKAYDSKLLSRLMTYLRPYKWTVVFAVVLTLLVG
                     PLEVVGPYLFGYTVDKYIVPGSNHSIALSAALRGVTFISLVSLAALLFSFAVQYLQVR
                     MMQNVGQQTLYDLRKEIFERMQRLPMSFFDRSPVGRLVTRVTTDVDALNDLFAAGIAA
                     MANDAVVLLSIGAVMMTKNWRLALATFAVLPLIMVSTWLFRNRVRDANRRIRTAIARI
                     NSFLQEHLSGMSIVQIFNREKKSQEQFAELNRFHMEAYKDAITAFAVFFPAVELFSTT
                     AIAIVFWYGGLLSFAGAVTVGTLITFMQYAQRFFRPIQDLSEKFNILQSAMAACERIF
                     RLLDEPLPAPPKEVPLSLAAPRGEIEFRNVWFAYTGGANPGDEDWVLRDVSFHIAPGQ
                     TLAIVGHTGAGKTTLIQLLLRFYDIQRGQILLDGMDIRKYNVHQLRRQFGIVLQDSFL
                     FTGTLESNVRLGDEDISRERVIAALREVGLGSLLDSLTEGVETNVTERGATFSVGQRQ
                     LISFARALAHNPRFLILDEATSSVDTKTELQIREALDRLLEGRTALVIAHRLSTIQHA
                     DRILVFHKGKLREQGSHQQLLAERGIYYRLYQLQYKDQELRVPFETAGSEAGTSVPGN
                     D"
     gene            complement(10597..12522)
                     /locus_tag="VG272_08390"
     CDS             complement(10597..12522)
                     /locus_tag="VG272_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF13737.1"
                     /translation="MSTAANTISVSELKSAPERVSGWTQLTRLYPYVMRHKTEVVIGF
                     VTQAGMGITGTLLPLILGAITDCIKGAETPLAQLGRLTQITLGPLLPYYHPKDPHTLA
                     VFCIALVAICTAQGVFSYWTRQILIGLSRDIEFDLRNDLLSKLVQMEPEFYVRNRTGE
                     LMSRCTNDLNAVRMVLGPGIMYSANTMATMVLAVILMFWISTTLSVYVLLPVPVVAVT
                     VWLFGRQIHALYGKIQASLAVLSAKAQENLAGVRVVRAYGQEESEIRGFDAPNKEYIN
                     RNLRLIYFWSMFMPLLQMLFGLTFILVLWQGGRLVVLDRITLGELIAFYTFMTRLIFP
                     MIALGFVTNIFQRGGASMGRLNYILDSEPTIDDAVAVKNVDEIRGEIDFRNLTFTYPT
                     DRSDIDGVKANGNGAPNKPVLENISLHVPAGSILAIVGPTGSGKSTLAALIARLWEAP
                     KGTLFVDGRPIREWPLETLRRAVGYVPQDTFLFSETLRENVAFGVDSATDEEIREAAD
                     VASILAEIGEFPAQFDTMVGERGITLSGGQKQRAAIARAVIRNPKILILDDSLSSVDT
                     GTEERILRRLDAILRQRTTILISHRISTVQHADQIVVLRDGQIIERGNHAALLAQGGY
                     YADLYQKQLLEEELARE"
     gene            complement(12519..13169)
                     /locus_tag="VG272_08395"
     CDS             complement(12519..13169)
                     /locus_tag="VG272_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13738.1"
                     /translation="MKTFKLRTALRTLPWIIALSMAGCQTAQQQPSVADHAATENAKR
                     ADEAHGVAEASLGKQSEILAQGDLALNGREQLLVVNRISGAALANKENASSPIYIMRA
                     AVLEKNDGKWSQILLCDEHLKNPNGYLGGSPKGRVTGWQLEFSKDEKLGLEMNFTPSN
                     KVGIENASADQGSGEKQPTFNVRWNKNTKRYQSFDASHERYLGEVPSLEAPESILK"
     gene            complement(13186..13785)
                     /locus_tag="VG272_08400"
     CDS             complement(13186..13785)
                     /locus_tag="VG272_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13739.1"
                     /translation="MKSWICASLAFAATCAAAASGQQAIPASAGKAPAPALRRMNETL
                     LAGLRPGRDTFAVAEKRLKPKEISGDSTSDSKEWRDACSGRSIRLAVDAKAVIQSVTL
                     TTVGKQEGKCSDRRPDFLDPKFWLTGLGLRMGDSQDRVVALYGEPNSGGPASKNGQEL
                     DLMYYQFDWAGSDVPQVMEILCARDTGRVVEITLAFPSL"
     gene            complement(13763..14437)
                     /locus_tag="VG272_08405"
     CDS             complement(13763..14437)
                     /locus_tag="VG272_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13740.1"
                     /translation="MPNRAYASIWVRDFTEENMLAHFGRFLAAVPLAAVPPGFTGLII
                     RAVDFAETPLEEFDLRGYVTTPEEVIALAAEHNAADVCYEVPARWDLWIRDKETASWK
                     IQPERMDIFCFGPEYDQGVYGESGHIMIDLGFEHLFTGHAGLLAAQSEKITEPQHPEE
                     ARFLMWMSQPQNLREYQEKTRENIKKLMGWMRAVEEALPVERFRLWSEGEENFEARLD
                     EILDLR"
     gene            complement(14555..15535)
                     /gene="add"
                     /locus_tag="VG272_08410"
     CDS             complement(14555..15535)
                     /gene="add"
                     /locus_tag="VG272_08410"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HWF13741.1"
                     /translation="MDFRSLPKIELHLHLDCSLSFSAVSRLNPSVTREEYQRDYIAPA
                     RCTNLADFLSRAPMGFRLMQTEDSLRLVTEDLFQQLVEDGVIYAEIRFAPLLHLENGL
                     SAERVVTTVERSVENLVRETGMQAGVILCTLRHFTEAQSKHTAELVEKFRGSRIVALD
                     LAGDEAGFPLDVHIGAYRYAREHGLFRTAHAGEGLGPESVWETLCMLEPQRIGHGTRS
                     IEDPKLVEHLLQKHIHLELCPTANVQIIPSIGRMEKHPIDRLYRAGVSLNINSDSRML
                     TPTTLTMEYEIVHRVFNWTEQDFQRSNLMGLEASFASDDVKQDLRKRFVQ"
     gene            complement(15982..16134)
                     /locus_tag="VG272_08415"
     CDS             complement(15982..16134)
                     /locus_tag="VG272_08415"
                     /inference="COORDINATES: protein motif:HMM:NF023180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13742.1"
                     /translation="MLLLIILLVLLFGGGGGYYGYSRWGAGGGIGILGTVLLIVLVFY
                     LLGGIR"
     gene            complement(16378..19221)
                     /locus_tag="VG272_08420"
     CDS             complement(16378..19221)
                     /locus_tag="VG272_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13743.1"
                     /translation="MAESNLHPDQEAQKRRSTRIVQAVPLTVTGVDALGRPFQERTST
                     LIVNCHGARYQSKHYVLKNMWVTLEVPHNEPGHSPRSVRGRVTWIQRPRTVRELFQIG
                     VELEVGGNVWGIAFPPSDWYPFPEAGVASSTEIPAPSQATELPHQVHEIPVHEKGAED
                     RVHLAPPPETQKPNVVSIPSPPESAEDNMRVLPMPAGEDASLQLARQVARLVDEAKQQ
                     IQIVARESANQAVAAETRPLLAALQTQLQNAAEKSVTAAAAAQFEKLQQETTQRIEKE
                     RETAVASVLDQLSTELDAKLGQARLQIDNQLAVVERTRRMDFEQQIQTQLQAAIHKME
                     SLSGSMGKNQDEVRAVMDQLRVSSAQAAADEIRRWKEQMDLRTAEAQARLAQMDQAAR
                     KLGEQIAAATVVGEVGWRGLLDADLEAANARWQEKMQSSVAEASQRAAEQIAKNAQDS
                     AQQVEQQLQQRFSTLSQAHSQITSEAESALMKLRAEIFTEALKGDEVISKFQESVAQL
                     EARRNEFSALLQTASDEWTRRGEAMLEAQSAELNRRAESTMTDMSQRLQPMLEAAGQE
                     SIEKLAAEFEQRFSPQIAHATEILSKLAFDSDRAEKAAAEYQQRIWQMSDLGLQESAA
                     RSKEILAGIEKEFAESARHASARWMAELETRATETSHGTFETLYKSADWYEKKIQTQM
                     QSTLEKGLDQAAARLREKAAEMSGLFASELDHYSRSYVEHAHGQMQENARDAAEQASQ
                     KMVEAGDSVAAQFTDRAAQLSGEQFDAFASRTRAEMEQNAAAMDAHTSQIRINLESDA
                     HGFASEFQNALSQHAEQTLTLGKQELGLQIDQARDALLIESQALERHFQSALTLHGNS
                     AVDEHKQRLENASNAWLLTTVTKLNQQSEHLISEMSDATEKKLKVVCENVFSEMGEIL
                     RQRLAGLASPFSSPAKPDASPSASSPSKPEEQK"
     gene            19848..20300
                     /locus_tag="VG272_08425"
     CDS             19848..20300
                     /locus_tag="VG272_08425"
                     /inference="COORDINATES: protein motif:HMM:NF041414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HWF13744.1"
                     /translation="MGNDSIASRKAHIAIHVKNVNASLEFYRKLFGIEPAKVRTGYAK
                     FDVANPPLNFALNEGTGNGQGALSHLGIQVGTTAEVLALRQKWQEQGLLTRDEMKTEC
                     CYALQDKSWVSDPDGNEWEVFAVLEDNLPEKAPSAKTCCPSDCCAPAA"
     gene            20430..20921
                     /locus_tag="VG272_08430"
     CDS             20430..20921
                     /locus_tag="VG272_08430"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HWF13745.1"
                     /translation="MGKPYNVLFLCTGNSARSIMAEAILTWKGHPYFVGFSAGSYPSG
                     IVRPEALQQIKASGILLENFRSKSWDEFVKPDAPQMDFVITVCDNAAGEVCPVWPGQP
                     ITAHWGVNDPAALKGSPAEIERAFREAFVILDRRISLFLSLPFSTLSQLAIKKEVGRI
                     GQR"
     gene            20955..>21257
                     /locus_tag="VG272_08435"
     CDS             20955..>21257
                     /locus_tag="VG272_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13746.1"
                     /translation="MATSQVQGQVCAPMPRASLSFLDRFLTLWIFLAMAIGVALGHFI
                     PQMATFVQDFQIGTTNIPIAIGLIIMMYPPLAKVRYEKLGEVFRNRRVLGLSLLQNW"
BASE COUNT         4761 a         5712 c         5839 g         4945 t
ORIGIN      
        1 ggggcgaggc ccgagtgaat cggtcttaaa gcgccaggcg acagccagct tattgaaatt
       61 ttccgcgttg acctgatcga gagcggagta gcgcgtgctt gcgagatctc caccgtaggt
      121 tcgccattct ccgttttttg cgccagtctg cgcattcaag gacacggcga tgcaaatgac
      181 aaaaaaagcc ggggcgaaaa aaaacacgca acgagagagc cgtctcatcg agttctcctg
      241 ggcatgcggg cacaatgctt gacgcgtgcc gttattgtag tgcgatatgc gccctattct
      301 gtcctcattt gcgaaaggaa acaagaattt acgttggaaa gtttaattca atatttttaa
      361 atgaaaagcg attcagtttt acgtagcaca ggctgaatcc tgtgctacat aaacccatgc
      421 acacgccttc gatcaaaaat atccgggaca aaaagcttta ggtagatttg atatttcggc
      481 ggcagcgttc caggagtgcc gtgtgtagcc gtttgcgaac atcttcgggg agcggaactg
      541 gttcagagtt gcaaaaaatc tggatggttt tgcgggtggt gtctaccacg actcggcaat
      601 ccgtgcagcg ggaaaggtgg agttcgagat cggttcgcat ctccgtatcg agtacgccgt
      661 cgaggtagct cgtcaattct agaataactt tcttgcagtt cactgcgttt cactccgtcg
      721 aaagtacttg ttgagttttt ggcggagctt gaggcgcgcc cgcaagaggc gcgatttcac
      781 cgccgctaca gaaatcccca gaatttgcgc ggtctcgtcc gtggaaagac cctcttcatc
      841 gcgcaacagg aaagcgacac ggaaaatcgg atcaagttca ccgattacgt tgttgagaat
      901 ctgattgagc tcggtctgcg cgaatttctt ttcaggagac ggaccccagt cgacgatctc
      961 gcggaacacg gaatcctcac cgtcgttatc cgtcagcggc tcatcgagcg acacctggtt
     1021 cgggcgccgt ttgcgcaatt tcatcagcgc ttcattgacg gcaatgcgca ccagccaggt
     1081 gtaaaaccgg gaatcgccct gaaagcggtc caaattctga taggacttca ggaaagcgtc
     1141 ctgcatcacg tcttccgcgt cctcgcggtt ttgcgtgatg ttcatggcca gccggaaaat
     1201 cttgcgctcg tagcgattta caagctcttc aaacgcagtg atatcgccgc ccttggcagc
     1261 agacaccaat agcgcttcgt cctcacggac gattgtggaa gccaacggtt tgggttcgct
     1321 agcgctcaag ttccatcgtg ctccgggcgg ctgcttgcac aggccagacg cactcatttg
     1381 gcaggttacc atagttctcc tttatatttc ctgccggcga cacatatatc ttcagatgca
     1441 gcgcctagcg tgctcgattc aggtatttat cttactaaca caatgcttaa aggtctgaac
     1501 tgtccgcgtg cctcgcagtg gtacaattca ttgaaactaa ttcagttcct aagttggagt
     1561 ttggtttcgc catatggtcc cgggcttgcc acagcgggag attgcggact tgtgctaatc
     1621 aagctgcttg gccaatcctg gatgctggga gtgcgcggta gtttgcgacc tgaaaatgcg
     1681 aactttacct gattatttac gcagcggtat gaaattgatc atcgtcggtt gcaatcccgg
     1741 cgacaggtcg gcacgcgtcg ggcactatta tgccgggcgt ggcaatgaat tctggccctt
     1801 gctctacgat tcaggagtcg tgccggaact tctcgatcat cgaaccgaca gacgcatgat
     1861 cgaatttggc attggcctca ccgatctcgt caagcgccca acacgcggag tagaggagtt
     1921 gactcgcgag gaatttgccg aggggcgcat cttgctctcg cagaagctgg agcagtttgc
     1981 gccacaagtg atcgcattca atggcgcggg cacgtttgag aacttcgcac agcgtccttg
     2041 caaacccggg cttcaaaaag aacggctgta tggggcacag gtgtttgttc ttccgtggac
     2101 aggcgccaaa agcgaaaacg gggaaacttc aaaaattcgt tatttcaagc agctagccaa
     2161 acttctttcc gaattgcccg attagagcgc ctgcacctca ccttgcatag agaattagtt
     2221 tttagctcga cggatcggtt cattccattt cctcgtcgaa atcaatacaa tatggcgcga
     2281 ggatatacct gctaaatgtt ttttgcgttg gaggctgtcc ggatttttca ttttccaggg
     2341 agataaaatc atggcgaaag caaaaatcaa gtttctgcag aaaccgtcct gcaccagttg
     2401 ccgaaaggcc aaagcttttc tggaaaagca aaaagtggaa ttggatttgc gcgatctcgg
     2461 aaaagacagg ctgtcggtcg aggaactcga taaattgatt ggcaagcgcg accatcgcac
     2521 ttttttgaat acgcgtaatg agatgtacag ggcgaagaaa atgaaagaga atccgccatc
     2581 gcgcgaagaa accctgaaat tgatggcggc cgaaccgaat ctgatccgca ggcccgtggt
     2641 gctgcgcggc agtgatattg tcctgggata tgatgaggaa gcgctaaaac gcatcgcgaa
     2701 ataaaactcg gcattaggaa acatcagaaa agatgattta tgggtatcgg cgaaaaaatt
     2761 gtgttgctgc aaggcgactt gacggagatg gacgtcgatg ccgtggtgaa cgccgcgaac
     2821 aatgatttgc agcttggggg aggagtggca ggcgcgattc gtcgcaaggg cggcgatcaa
     2881 attcagcgcg aatgcaatga gattggcagt atcccgattg gcggcgccgc catcaccacg
     2941 ggcggcaagt tacgcgcgcg ctttgtgatt catgcagcga gcatgcaatt gggcggagaa
     3001 accaccgcgc acgcgctgcg aagctcaacg gcgcattcct tgcgcatcgc tgcggagaag
     3061 gggctgcgca caatcgcatt cccggctgtg ggcaccggca tcgcgggctt tgcaatcccg
     3121 gaatgcgcgc gaattatgct gcgcgaggta gtggagcatc tgaagaagcc cacctctctc
     3181 gaaaaaatct atttcgtatt atttgatgac cgggtgcttt ccgaatttga aaaggcgcgc
     3241 gcggaaatgg ctgccgccgt cgaattcgat ggcgcggctc aaacagaaca aaaatgaaag
     3301 aactgactga aaaagttctt gccgaactca aacaatatga gcacccgctt tttgttttcg
     3361 atgcgcgccc ggctgcggaa ggcgtcgaga ttctcatcca ctattcgccg cccgagccac
     3421 aggttcacac gtatatattt ctgatgcggc ctcgcgaaat cgaaagtaag caatttccct
     3481 ggatttttca gaaacaactt tacgattgcc tgcacgattt catcgtcgag atgtttacgt
     3541 gcaatccgca gcgccaggac gtatgacgat ccctccatct ttcggagcgg gcgcaggaat
     3601 cgaatccgct aagccgggag agcactcgcg cgcgctaatt gaaattctcg cgcggcgtat
     3661 ccgcgcgggt ggtcccataa cgttcgccga gttcatgcgc gaatgccttt atcatcctat
     3721 ccatggctat tattcgcggg cgagcgcacg gtgctttggc gattattaca cgagtgtcga
     3781 tgtgcatccg atttttggac gattgctcgc gcggcaattt gcggaaatgt ggaagctgct
     3841 tggttctcct cgcacgtttg cggtcgtcga gtcagccgcg ggcgtggggc gtcttgccgg
     3901 tcacatcctt gatttcagcg cgcgcgaatt gcctgaattt tacgcagcgc tgcactatgt
     3961 tgcggtcgag cgctccagcg cgcgccgggc acaccatgct gcgcaaatgg gatctcacgt
     4021 caccgcagga cgcgcttcga ccgcggacga aattccgggc gctatttcca caggttgcat
     4081 tttttcaaat gaattgctgg atgcgttgcc cgtgcaccgc gtaatcatgg aacacggatc
     4141 gttgcgtgag atttttgtgg aattcgagaa tgcacgattc atcgagcagc gagaaaaact
     4201 atcgatgccg gcgctggagg aatatttcag cgagcaggga attacgcttg ccgatgaaca
     4261 gcaggcagaa gtgtgctttg aagcctgcga ctggattgaa tgtgccggac gcgctttgga
     4321 gcgcgggttt gtgctgacca tcgattacgg gcacgaagca cccattcttt ataacgaaca
     4381 tcacaatcgc ggcacgctgc tcgcctaccg agctcacacg gttacagaaa atattctgga
     4441 tgcgccgggg gagcaggacc tgacgtcgca cgtaaatttt actgccgtgg atttgtgggg
     4501 gcgccgagtg ggcctcgctt gcacgggact cgttacgcag tctcaatttc tggtcgctct
     4561 cggtcgagag aatgagttcg ctgatttata tgagccggga cagccggaaa ttgaaaaatt
     4621 gcgggcgagg ctgttgctga aaaatctgat tcatccggaa ggactgggtg aaaagtttca
     4681 agtgctcatc caacacaaag gaattgcgac gccgcagctc accggattgt caggatatta
     4741 attgattttg cgcggttcga tttttagcgg cgcggacttt agagtctgtg tgagaacggc
     4801 aaaattgaaa cttcagcggc taaagccgca taattttgca ggactcatgt cgcggctaaa
     4861 gccacgacca caaagattct gcagttctca cgcagactct ttagcctgcg gattttcgtc
     4921 tttgcaagga ccagaccccg caggccgaag cccgcgctac tagaagccgc tcacgaacca
     4981 gcttcgctgt aagccttctt catggatttt tcggcttcgg cttgcgcgcc gcccgtatcg
     5041 tccgccgtct gcttggcttt gttgtattca ttaaccgcgc gtgtgcggtc tccctgaata
     5101 tcgtaaatcc tgccaaggta gatatgcgac cagacttccg tccatttcgt ggtcagatcc
     5161 gtagcgccgt cgagcgcatc gcggaaggat tgcgcagagg cggcataatt ccgctggtag
     5221 aagaacgctt cacccatgcg aaattcggct agcgcgttca ggcgatcctg ctcaagcgcc
     5281 ttttggtact ggatgacagc gtcgtagtag cggccctgcg acgccagcga ttcgccgcgc
     5341 gcgataattc ccctcacgcg caagcgctga ttcgatttca aaatgtagtc gtgcggatcc
     5401 aaaataattc cgttcggctt gggccgcccg aaagtttcca cagtaaatgg cgattctttt
     5461 cctgaaatcg taatcgtctt gaattcagga tttccttccg tctgcacttc aatctcgacg
     5521 ttcatgtgga agacgtccag attttgtttc gcttttccga caatccggaa tccttttttg
     5581 gtacggtaaa tcacgtattc gatattgaat tccgggactc ctgtcgagtg cagccattgc
     5641 gtgaagaagg gacgcaagct ggtcggttca tcggacgctg cgggagcgtt gcccatttta
     5701 aattcagcag gcgcggcctt gcccatgtgc gactccgcga gctgttcaaa atcctgaatg
     5761 cgcgcggatt ttccggcgta tcgcgtatag aaatccttga gaagcgcatt aaaatttgca
     5821 tcgccgataa tcgcgcgaag catgtgaaac acgatggcgc ctttattcac gacgacggat
     5881 tgatattcct cggtgccatc ttcgaggcgg cccgcatcgg caatgggagc cgaatcgtca
     5941 aacatcagcg cgcctaccgc aaaatcctcg atcgccttat tcagcgcgtc tttcccagag
     6001 gtttgctcga cgtacaaacc ttcggcgtag cgcgagagcc cgtccgtgat ccacgcatcg
     6061 gacggcgttg cagccgtgac ctgtgaaccc caccattgat gagctgcgag gtcagccagc
     6121 agtcgctcgt ttggtttcac ggaccattgc cgcgcgctta ccagcaacag gtttggcgcg
     6181 gcgtatccgt ccactgtgcc atcgggcagt tgcgcaaccg tcatactcgg attgggcagc
     6241 ggaccaaacg tgtcattaaa atagtcgatg acttgtgcga gagagtttgc gtaggccggg
     6301 gccgtatttg ccgccgctgg cggcgtgtaa atcgaaaatg ccatgccttg cgcgtgttca
     6361 ggatttaatt gcagcggcga aataacgaag gtgccggcgg cttcgggttt gtccactcga
     6421 taggtataga gcatgcgttc attttccatc ggcggaggag gcggcgcgtt cataggcact
     6481 acaggcgccg ctgattttga gtgcgaattg ccgagcgcag aaggagcgag cgcaggtttt
     6541 gaatttaccg gggcagcggc tggctttggg gtgaccgaag ttggtgcacc tgccaaaatt
     6601 ccggttccga cgacggtcat gttgccgggc acttcgatct gaaaaattcc tgtgtaacga
     6661 ttcgacggaa aatcggtcag cggaaaccag cgcgaaggaa gcaataagta gcctccgtcc
     6721 ttagctatga aagccatgcg cgtgccccgt tcgggattca acacgcggct ggaaagcggg
     6781 ccaccgtagt cgaaaaacag ggtgacctgt gatcccgcgg gcacggtgtc gttcagagtg
     6841 acgcgcagct tctgcgcgga catgtcgtcg cggtcaaaat ttacaggttt accggagaga
     6901 tcgcgaatcg aattaacacg caaattttga ttcaattcca cttccacgac gcgcgacgca
     6961 tctcgtgccg cgaagtccac tttcgcttga gcgttcatca cctgcccgag tgtgtcgagc
     7021 gatgctctaa tttcgtagtt cgttgcgcgg aaggcagcga tgggggcggt ctgaacaggc
     7081 gcaactccgc ggctgggacc ttgaggcgct cccggagatt gggcgcttga gggcagtgcg
     7141 aagcttgcga ggctcgccag aaccagcaag attgtaaatt tcgacatgag attaagatgc
     7201 cttctttccg gaaaatacta tgtaaaacag taggatacca acttgacccg atgagttgcc
     7261 agccctagct ttctttcgcc acgaactgga cgcgtgtctc gctaggacat tcagtgcctg
     7321 ccaaaatatc ggcggccact acgaaaccga ttttgtggcg gccttcgtct cgtgcatttc
     7381 gaggcggaag ggcactgtcc cgccaagcca ggccgatcct ttcgagctag ggctgattta
     7441 acatgcttac aatcaagttg gctgcgcgtt caatcgcgcc tggagggcca agcttttcgc
     7501 gtatttccgc taggccgagg cgcatttccg ctcgcgcgcc ggccgaatcg agcaggcgaa
     7561 tggcttcgcc gaagacccgg tccggagtga attccttctg tacaagctca ggcacaactc
     7621 gcttgcctgc aatcagattg accatggcga acatcggcgt tcgaaccagc aggcgggcta
     7681 tccccgcggt gagcggcgag aggcgataca caacgatcat cggcgcgtcc atcagagcgg
     7741 cttccactgt agcggtaccg ctggatacga ttgcgaggtc ggcagcgccc aaagcgtcat
     7801 aggttgcgcc ttctaccaca tttacgggca cctctgcgct gagatattgt gcgacctgcg
     7861 gttgtttcat gcccggcgcg agagctagaa caaattgcac ctcgcgctct ttttgaatcc
     7921 gagcgcaggc ttgcatcagc gtgggcatgt ggtgcgcgat ttcgccagaa cggctcccgg
     7981 gcagaattgt gacgatcggc ttgctcgcgt ccagaccgta agcggcggca aactcggcgc
     8041 gcgttcgttt tgcggaaacg tttccaacaa gcgggtggcc gatgaaatct gctttcacgc
     8101 cgcgcgagcg ataccattgt tcttcgaacg gaaatatgca cagtccgcgc acgaatcgtc
     8161 gccgcacaag attggcgcgc cagggacgcc atgcccagaa ctgagggcac acaaaataga
     8221 tgcactgcac gccctggcgc ttcagcgcgc gcgccatgcg cagatggaag ccgggaaagt
     8281 ccgtgaggat cgcgaaacgt ggcttacggc gcgatgattc acccaccaag cgattccaga
     8341 cgcgcttcag cgcgggatat tttttcgcga tctccacgat tcccacaacc gatacttcag
     8401 agctatccgc gacgatttcc acgccggcct cgcgcatccg catgccgccc atgccaaaga
     8461 gatggacgtc tgcgcgggat tgcagggccg ctgccagccg cgccgcgtac atgtcaccgg
     8521 aagcttcccc cgcggaaatt agtatttgtg taggaggcat tttatgtagc gcaggctgaa
     8581 gagtccgtgt gagaactgca aaattgaagc ttcagcggct aaagccgcac tcattttgca
     8641 ggacttatgt cgtggctaaa gccacgaccc acaaagattc tgtagttctc acacagactc
     8701 tgaagcccgc gccactaaaa ctttaatcat ttccgggaac ggacgttccc gcttcgctgc
     8761 ctgctgtctc gaacggcact cgcagctcct ggtctttgta ctggagctgg tacaggcggt
     8821 agtaaatccc gcgctcggca agcaattgct ggtgcgaacc ttgttcgcgc aatttgccct
     8881 tgtggaaaac gagaatgcgg tcggcgtgtt gaatggtgct aaggcgatgc gcgataacga
     8941 gcgcggtgcg gccttcgagc aagcgatcga gagcctcgcg gatttgaagt tcggtcttgg
     9001 tgtccacgct ggacgtcgct tcgtccagaa tcaggaagcg gggattgtgc gccagagcgc
     9061 gcgcaaagct gatcagctgg cgctggccca ccgagaaagt cgcgcctcgt tccgtgacgt
     9121 ttgtttcgac gccttctgtc aacgaatcca gcagcgagcc aaggcccact tcacgcagcg
     9181 cggcgatcac acgttcacga ctaatgtctt cgtcgcccag gcgaacattc gattccagcg
     9241 tgccggtgaa gagaaatgaa tcctgcagca cgatgccgaa ttggcggcgc aattggtgaa
     9301 cgttgtactt tcggatgtcc atgccatcga gcaaaatctg gccgcgctga atatcgtaaa
     9361 agcgaagcag cagttggatg agcgtggttt ttcccgcgcc cgtgtgtcca acgatggcga
     9421 gcgtctgtcc cggagcgata tggaacgaga catcgcgcaa aacccaatct tcgtcgccag
     9481 gattcgcgcc gcctgtgtag gcgaaccaca cattgcggaa ttcgatttcg ccgcgcggag
     9541 ctgcaagcga gagcggaact tccttaggcg gcgcgggtag cggttcatcg agcaatctga
     9601 aaatgcgttc gcaggcggcc atagccgatt gcagaatgtt aaatttttcg ctcagatcct
     9661 gaatcggacg gaagaagcgt tgcgcgtatt gcatgaaagt gatcagcgtg ccaacggtta
     9721 cggcgcccgc gaacgagagc aaacctccat accagaaaac gattgcgatc gcagtagtgc
     9781 tgaagagctc gacggcaggg aagaaaacag caaacgctgt aattgcatcc ttgtacgctt
     9841 ccatgtggaa gcgatttaat tcggcgaatt gctcttgcga ttttttctcg cggttgaaga
     9901 tctgcacgat actcatgccg ctgagatgtt cctgcaggaa cgaattaatg cgcgcgatgg
     9961 ctgtgcggat ccttcggtta gcgtcgcgaa cgcgattgcg aaagagccat gtggaaacca
    10021 tgatcaacgg caacacggcg aaggtggcca gcgccagccg ccagtttttg gtcatcatga
    10081 ccgctccgat gctcagtagc acgacagcgt cattggccat ggccgcaata ccagcggcga
    10141 acaaatcgtt cagcgcatct acgtccgttg ttacgcgcgt cacgaggcgc ccgaccggac
    10201 tccgatcaaa aaaactcatc ggaagccgct gcatgcgctc gaagatttct ttgcgcaaat
    10261 cgtagagcgt ctgttggccc acgttctgca tcatacgcac ttgcaaatac tgcaccgcaa
    10321 aactgaacag cagcgccgca agcgaaacga gagagataaa cgtaacccca cgcaaagccg
    10381 cggaaagtgc gatggaatga ttggagccgg ggacaatgta tttgtcgact gtataaccga
    10441 agagataggg cccgacaact tcgagcggcc cgacgagtag ggtaagaacg acggcgaaca
    10501 caaccgtcca cttatacggc cgcagatacg tcatcagccg gctcaaaagt tttgagtcgt
    10561 aggcttttcc tagagcttcc tcttcacgga agtcgctcat tcgcgcgcca attcttcctc
    10621 gagcaactgt ttctggtaca gatcggcgta atagccgccc tgcgccagaa gcgcggcatg
    10681 attgcctcgt tcaataatct gaccgtcgcg cagcaccaca atttgatcgg cgtgctgcac
    10741 ggtggaaata cgatgcgaaa ttaatatagt tgtgcgctga cgcaaaatcg cgtccagccg
    10801 ccgcaggatg cgctcttcgg ttcccgtgtc cacgctggaa agggaatcgt cgaggatcaa
    10861 aattttagga ttgcgaatga cggcgcgggc aattgcggcg cgctgttttt gtccgccgga
    10921 aagcgtgatt ccacgctcgc ctaccatggt gtcgaactgc gcggggaact cgccgatttc
    10981 ggcgagaatg ctggcaacgt cggcggcttc gcgaatttct tcgtccgtcg cgctgtccac
    11041 gccgaacgcg acattttcgc gcaacgtttc gctgaaaagg aacgtatcct gcggcacgta
    11101 gccgaccgca cggcgcagtg tttcgagcgg ccattcgcgt atggggcggc catcgacgaa
    11161 aagtgttccc ttgggggctt cccacaaacg ggcgatcagt gcggccaacg tggattttcc
    11221 gcttcctgtc ggtccgacga ttgcaagaat cgatccggct ggaacgtgca acgaaatatt
    11281 ttcaagaact ggcttgttcg gtgcgccgtt gccgttggct tttacaccat cgatgtcgct
    11341 tctgtcggtc ggataagtga aagtcaaatt ccggaaatca atttcgccgc gaatttcatc
    11401 gacgtttttg actgcgactg catcgtcgat cgtcggttcg gaatcgagga tgtaatttag
    11461 ccggcccatg gacgcgccgc cgcgctgaaa aatattggtg acgaagccca atgcgatcat
    11521 cgggaaaatc agtcgcgtca tgaacgtgta aaacgcgatc aactcgccga gcgtaatgcg
    11581 atccagcacg acgaggcggc cgccctgcca cagaaccagg atgaatgtga gcccgaacaa
    11641 catctgaagc aagggcataa acatgctcca gaaataaatc aggcgcaggt tgcgattgat
    11701 gtattccttg ttgggcgcat cgaatccgcg aatttccgat tcttcctggc cgtacgcacg
    11761 caccacgcgc acgcccgcca gattttcctg tgcctttgcg gaaagcacgg cgagcgaagc
    11821 ttgaattttt ccgtacagcg cgtgaatctg gcgtccgaaa agccacacag tgacggctac
    11881 taccggcacg ggtagcagaa cgtaaacact cagcgttgtg gaaatccaga acatcaggat
    11941 cacggcgaga accatcgtcg ccatggtgtt ggcgctgtac atgatgccgg ggccgagcac
    12001 catgcgcacg gcgttcaaat cgttcgtgca tcgtgacatc aattcgccgg tgcgattacg
    12061 cacataaaat tccggctcca tttgcacaag tttcgataga agatcgttgc gcagatcgaa
    12121 ctcaatgtcg cgcgaaaggc cgatcaaaat ttgccgcgtc cagtaggaaa acacgccctg
    12181 cgcggtgcaa atcgccacga gcgcaataca aaacacggcc agtgtgtgcg gatctttcgg
    12241 atggtagtaa ggaagcagtg gtcccaatgt aatttgcgtg agccgaccaa gctgcgccag
    12301 cggcgtctcg gcacctttga tgcaatccgt gatcgcgcca agaatcagcg gcagcagcgt
    12361 gccggtaatt cccatgccgg cctgcgtgac gaagccgatc acgacttcgg tcttgtgccg
    12421 catcacgtac ggatatagac gggtgagctg cgtccatccg gagacgcgct ccggcgcgct
    12481 cttcagttcg gatacggaga tggtattggc cgcggtactc atttcaggat agattcggga
    12541 gcttccagcg atggtacttc gcccaggtac ctttcgtgag acgcgtcgaa cgattggtag
    12601 cgtttcgtat ttttattcca gcgtacatta aaagtgggtt gtttctctcc ggaaccctgg
    12661 tccgcactgg cgttttcgat gccaacctta tttgaaggcg taaaattcat ctccaaccct
    12721 agcttctcgt ctttgctgaa ttccaattgc cagcccgtca cccgcccctt tggcgagcct
    12781 ccgagataac cattcgggtt tttcaaatgc tcatcgcaaa gcaaaatttg agaccacttg
    12841 ccatcattct tttccagcac ggcggctcgc ataatataga ttggcgaact tgcattttcc
    12901 ttgtttgcca gtgctgctcc cgaaatacga ttgaccacca gcaattgttc gcgcccattc
    12961 agcgcgagat cgccctgcgc cagtatttca gactgcttgc ccaaggatgc ttcggcaacc
    13021 ccgtgggcct catccgcgcg cttggcgttt tccgttgctg catgatcagc gacggatggc
    13081 tgctgctgag ccgtctggca gcctgccatg gaaagcgcga tgatccatgg cagagttcgc
    13141 agagccgtgc gaagtttaaa agttttcatc gtggatcgat ttgcactata agcttggaaa
    13201 agccagagta atttccacca cgcgcccagt atcccgcgcg cacaggattt ccatcacttg
    13261 cggtacatcc gacccggccc aatcaaattg ataatacatg agatcgagtt cctggccatt
    13321 cttagatgcc ggcccgccgg aattcggttc tccgtaaagc gcaacgactc gatcctgcga
    13381 atcacccatt cgcaaaccga gccccgtcag ccaaaatttt ggatccagaa aatccgggcg
    13441 acgatcgctg cattttccct cctgtttgcc aaccgttgtc aaggtgacgc tttgaatcac
    13501 ggccttggca tccaccgcca ggcgtatgga gcggcctgaa cacgcatccc gccattcctt
    13561 ggaatccgaa gttgaatctc cggaaatctc tttcggcttc aggcgttttt ctgccacggc
    13621 aaacgtatca cgtccgggcc gcaatcccgc cagcagcgtt tcattcatgc ggcgcaatgc
    13681 cggagcagga gctttgccgg cgctggcagg gatagcttgt tgtccagacg ccgcggcagc
    13741 acacgtcgcg gcaaacgcca agctagcgca aatccaagat ttcatcgagc cgcgcttcaa
    13801 agttttcttc tccctcggac cacagacgga agcgttccac gggcaaagcc tcctccacgg
    13861 cgcgcatcca gcccatgagt ttcttgatat tctctcttgt tttctcctga tattcgcgca
    13921 aattttgcgg ttgcgacatc cacatcagga atcgagcctc ttccggatgc tgtggctcag
    13981 taattttttc cgactgtgcg gcgagtagcc ctgcgtggcc cgtaaacaaa tgctcgaagc
    14041 ccaggtcgat catgatgtgg ccggactcac catatacgcc ctgatcgtat tccgggccga
    14101 aacagaaaat atccatacgc tcgggctgaa ttttccagct agcggtttcc ttgtcgcgaa
    14161 tccataggtc ccagcgcgca ggaacttcat agcacacatc agcggcattg tgttcggcgg
    14221 ccagcgcgat cacctcttcg ggcgtggtca cgtagccgcg caaatcaaat tcttcgagtg
    14281 gagtttccgc aaaatccacg gcacgaatga tcagccctgt aaaacccggc gggacagcag
    14341 ccagcggcac cgccgccaga aaacggccga agtgcgcgag catgttttct tccgtgaaat
    14401 cacggaccca gatactggca taagcacgat tcggcattcg gatattatac aggtaagggg
    14461 gctaaggact gaaaatcgcc gacaggcccg taatgatcgg aatatcattc ttggctgcga
    14521 aacccgacgt gccaacctga aggccgacgc tacgctattg cacaaagcgt tttcttagat
    14581 cctgttttac gtcgtcgctt gcgaagctgg cttccagccc catcaaattc gagcgttgaa
    14641 aatcctgctc agtccaatta aaaacgcggt ggacgatttc gtactccatg gtcagcgtcg
    14701 tcggcgtcag catgcgactg tctgagttga tgttgaggga aactccggcg cggtagaggc
    14761 ggtcgatggg atgtttctcc atcctgccaa tcgaaggaat aatttgaacg tttgccgtgg
    14821 gacaaagttc caggtgaatg tgtttttgga gtagatgttc caccagtttg ggatcttcga
    14881 tgctgcgggt tccgtgaccg atacgttgcg gttcaagcat gcatagcgtt tcccacacac
    14941 tctccgggcc cagcccttcg ccagcgtgcg ccgtgcgaaa taagccatgt tcgcgcgcgt
    15001 agcgatatgc gccaatgtgc acgtccaagg ggaatcctgc ttcgtcgccc gcaaggtcga
    15061 gcgctacgat tcggctgcca cgaaattttt ccacgagttc agccgtgtgc ttgctttgcg
    15121 cttccgtgaa atgcctcaac gtgcaaagaa tcacgcccgc ctgcatacct gtttcgcgga
    15181 ccagattctc gactgatctt tcgacggttg taaccacgcg ctcggcgctc aacccattct
    15241 caagatgcag caggggagcg aagcgaattt cggcataaat cacgccatct tcgaccaatt
    15301 gttggaataa atcctcggtc acgagtcgca gggaatcttc ggtctgcatc agtcgaaaac
    15361 ccatgggtgc gcgggaaaga aaatccgcga ggtttgtgca gcgtgcgggc gcaatgtaat
    15421 cgcgctgata ttcttcgcga gtaacggacg gatttagcct ggatacagca ctgaagctga
    15481 gtgagcaatc cagatgaaga tgcagctcaa tttttgggag cgaacgaaaa tccatctggg
    15541 aagaataacc tgaagggaaa gggctggtcc atcaaaccta gaaaaggaaa tgatttgatt
    15601 tggcaggaac ccgccctctc cgtcccgaag aacactgttc atcgagaacg ttctccgtga
    15661 acgggacgga gaggccgcta caaaaccgca acgcctgata tcgttggttt tgtagaggct
    15721 ggccgcctgt tgggcgggcg gttttttctg cgtcaataat ttctattgat gcatcgtaaa
    15781 aaaaatatat cggtgaataa accggggtca aaagcaaaaa tattaactta aacggaagaa
    15841 acagctggaa gcagagtcgg catcaactcc atgggagcgc cgagtccatc agacttttcg
    15901 cgccgccctt ccagctgttt cgtgctttct tttgccatgt tttctccctt atccgaatac
    15961 tgccactcta aatttggacc actaacgtat gccgcctaac aaatagaaga ccaggacgat
    16021 gagaaggacc gttccaagaa tcccaatgcc tccacccgct ccccatcgcg aatagccata
    16081 atagccgcca ccgccgccga acaagagaac cagaaggatg atcagcaata acatcgcaag
    16141 cctcctttca aacggttgac ttcaatagct cgccaagtta cttcgtttcg tatattccat
    16201 gtgcagacga atccccacaa tgaagacaac cctatattta tcagtccaaa tcgctgtgga
    16261 attcaactgg cgattattgg acatagcgga gaacaactga gttcaagcgg aattcgagag
    16321 gagggtaaga ccttatagat ttcatttcat tgaatcgaaa ggattgggaa ccgcgcgtta
    16381 tttttgctct tctggtttgg aagggctgga agcagaaggc gatgcatcgg gttttgcggg
    16441 actggaaaat ggggaggcca atccggcaag gcgctggcga aggatttcgc ccatttccga
    16501 aaacacattt tcgcaaacca ctttcaactt cttttcggtg gcgtccgaca tttcggaaat
    16561 taggtgttcg gattgttgat tcagtttggt gaccgtagtc agcagccatg cgttcgacgc
    16621 gttttccagg cgctgcttgt gctcgtccac ggcggaattt ccgtgcaagg tcaacgcgga
    16681 ttggaaatgg cgttccagag cttgcgactc gatgagcagg gcatctctgg cctgatcaat
    16741 ttgcaggccg agttcttgtt tgccgagggt cagcgtctgt tccgcatgct gcgacaaagc
    16801 attctggaat tcggaggcaa agccgtgagc gtcgctttcg agattgatgc ggatttgcga
    16861 agtatgagcg tccatcgcgg cggcattctg ttccatttcc gctctggttc tggacgcaaa
    16921 ggcgtcgaat tgttcgccgc tgagctgtgc ggcgcggtcc gtaaactggg cggcaacgga
    16981 atcgcctgct tccaccattt tctgagaagc ctgttcggcg gcgtcgcggg cattctcctg
    17041 catttgtccg tgagcgtgct cgacgtaact tctgctgtaa tgatcgagct cgctggcgaa
    17101 cagcccggac atttcggcgg ctttctcgcg cagccgtgca gcggcctggt ccaggccctt
    17161 ttcgagcgtc gactgcatct gggtttgaat ttttttctcg taccagtcgg ccgacttgta
    17221 cagagtttca aatgtgccat gcgacgtttc cgtggcgcgc gtctcgagtt cggccatcca
    17281 tcttgcggaa gcatggcgcg cggattcagc gaattctttc tcgatccccg cgaggatttc
    17341 cttgctgcgc gcagcggatt cctgcaatcc aagatcggac atctgccaga tacgctgctg
    17401 gtattccgca gccgctttct ccgcgcgatc gctatcgaat gccagtttgc tcaatatttc
    17461 cgtagcgtgg gcaatctgcg gcgaaaaacg ctgttcaaat tcggcggcca gcttttcgat
    17521 ggactcctgg cccgcggctt cgagcatagg ttgcagccgc tgggacatgt ctgtcatcgt
    17581 cgactccgcg cgccgattca attccgcgct ttgcgcttcg agcatggctt cgccgcgccg
    17641 tgtccactca tccgacgccg tctgcaacag cgctgagaat tcattgcgcc ttgcctccaa
    17701 ctgcgcgacg gattcctgaa attttgaaat cacttcgtcg ccttttagcg cttcggtgaa
    17761 aatttcagcg cgcaatttca tgagcgcgct ttcggcttcg ctggtaattt gcgaatgcgc
    17821 ctggctcagc gtgctgaaac gttgctggag ttgctgctcg acctgttgcg cggaatcttg
    17881 cgcgtttttt gcgatttgtt ccgctgcgcg ctgcgatgcc tccgcaaccg acgattgcat
    17941 tttctcttgc caacgtgcgt tggccgcttc gagatcggcg tccagcaggc cgcgccatcc
    18001 aacttctcca accacggtgg cagcagcgat ctgttctcca agttttctcg ccgcctgatc
    18061 catttgcgcg agtcgcgcct gggcttccgc cgtgcgcagg tccatctgtt ctttccagcg
    18121 ccttatttcg tccgccgcag cctgcgcgga tgaaactcgc agctgatcca tcacagcgcg
    18181 aacttcatcc tgatttttgc ccatgcttcc gctgaggctt tccatcttgt ggatcgcagc
    18241 ttgaagctgg gtctgaatct gttgttcaaa gtccattcgc cgtgtgcgct caacaaccgc
    18301 aagttgattg tcaatctgca atcgggcctg gcccaatttc gcgtccagtt cggtggacaa
    18361 ttgatcgagc acactggcca cggccgtttc acgttctttt tctattcgct gagtggtttc
    18421 ctgctgtaat ttttcaaatt gtgcggcagc cgcggcggtt acggatttct cggcagcatt
    18481 ttgcaattgt gtttgcaaag cggcgaggag gggtcgcgtt tccgctgcca ctgcctgatt
    18541 ggcactctct cgcgcaacaa tttgaatttg ctgtttcgct tcgtccacca atcgcgccac
    18601 ctgccgggcg agttgcagag aggcatcttc ccctgcgggc atgggaagaa ctcgcatatt
    18661 gtcctctgca gattcgggag gagagggaat cgaaacgaca ttgggctttt gcgtctctgg
    18721 tggaggcgcc agatgaaccc tatcctcagc acccttttca tggaccggta tttcatgtac
    18781 ctgatgcggc agttcggtcg cctgcgatgg cgcagggatt tccgtgctcg acgcaactcc
    18841 ggcttcaggg aacgggtacc agtccgaggg agggaaagca attccccaaa cgttaccgcc
    18901 aacttccagc tcaaccccaa tctgaaaaag ttcacgcacc gtgcgcgggc gctgaatcca
    18961 cgtcacgcgc ccccgcaccg agcgaggcga atgccccggc tcattgtgag ggacttccag
    19021 cgtgacccac atatttttca aaacataatg cttcgattga tagcgtgcgc cgtggcaatt
    19081 aacgatcaga gtggaagtgc gttcctgaaa cgggcggccg agcgcatcca caccagttac
    19141 cgtcagcgga acggcttgta caatgcgcgt gctgcggcgc ttttgtgctt cctggtcggg
    19201 atggaggttg gattccgcca caattactcc tactgagcta agtccaaagg gtagcaccac
    19261 atcagccaat cgtcgagact catggggtgc cgatttcgta aaggttacac cccgtaagag
    19321 taatccacct agaggttctg tattatcgag caatggtagg gaggattagt ggttgcggcg
    19381 cttgctttgg ccggtcaacg gaacttgggg tgcaacagct cgtctcgagc ttcgccagat
    19441 catcccgcat ttcaggcatt ttccgcaaat gggtcaaagt ttctttcaat ataccggacg
    19501 ccatcacgtc ttggggaagg accagacggt aatgtatcca gcgcccctgc ctgcgcgccg
    19561 cgacgattcc tgcctttctt aaataagcca agtgacgtga gatttttggc tggctgatcc
    19621 gcaatatctc tacgaaaaag caaacgcata tctccttgtc cgcaatcagg ttgaggagac
    19681 gaagcctcgt gcggtcggct aaagcacgaa agagcagatc gaggacaaag ggattgtttt
    19741 tggaagccac agaagcatta tatatgcctt gacagatatg atcaattgcc gttaactgac
    19801 tcatatacgc atatgcgtat atatgttctc tcgttcgagg aggctctgtg ggaaacgatt
    19861 caatagcatc gcgcaaggct catattgcaa tccatgtcaa aaacgtgaat gccagtcttg
    19921 agttctaccg caaacttttc ggtattgaac cggccaaggt aaggactggc tatgcaaaat
    19981 tcgatgtcgc caatccgccg ctcaattttg ctttgaacga aggcaccggg aacgggcagg
    20041 gagccctgtc gcacctgggt atccaggttg gtacaacggc agaggtcctg gccttgcgcc
    20101 aaaaatggca agagcaaggg ctgctgacgc gcgacgaaat gaagacggaa tgctgctatg
    20161 cgcttcagga taaatcgtgg gtcagcgatc cggatggaaa tgaatgggaa gtcttcgcgg
    20221 tgcttgagga taatcttccg gagaaagcgc cttccgcaaa aacatgctgt ccctccgatt
    20281 gctgtgcgcc ggcggcgtag aggcgaagtt ccgagatgaa gaatttgcag gtgtagcgct
    20341 cgccttcagg cgagcgctac gaaaaaccgt cgcgttatta gggcgatctg agttttttcc
    20401 actgagccag agccaccaga ggaaacacaa tgggcaaacc gtacaacgtt ctctttttgt
    20461 gcactggaaa ttccgcgcgc tccatcatgg cggaggcgat tctgacctgg aaggggcatc
    20521 cgtactttgt cggcttcagc gccgggagct acccatcagg gatcgtaagg cctgaagcgc
    20581 tgcaacagat caaagcctcg gggatactct tggaaaattt tcggagcaag agctgggatg
    20641 aattcgtcaa gcctgacgcg ccgcaaatgg attttgttat cactgtttgt gacaacgctg
    20701 ccggcgaagt ttgccccgta tggccgggcc agccgataac ggcgcattgg ggagtgaacg
    20761 atccggcagc gttaaaggga tcgcccgcag aaatcgaacg cgcgtttcgc gaggcttttg
    20821 taattctgga ccgccgaatt tcgctttttt tatctctacc attctcgact ctcagccaat
    20881 tagcaatcaa gaaggaagtt ggccggatcg gtcaacgatg agaaacatca cgcgttctta
    20941 ctgaggtgaa taggatggca acttcccagg tacaaggcca ggtttgcgcc ccgatgccgc
    21001 gcgccagcct gtcattcctt gatcgttttc taaccttgtg gatttttttg gcaatggcca
    21061 ttggagttgc gctgggccat ttcattccac agatggcgac ctttgtgcaa gattttcaga
    21121 taggcacaac gaacatcccc attgccatag gcctgatcat tatgatgtac ccgcctttgg
    21181 caaaagtgcg gtatgaaaag cttggtgaag ttttccgcaa tcgccgcgtt cttgggcttt
    21241 cgcttctaca aaattgg
//
LOCUS       DASZMB010000183         5761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3217715, whole genome shotgun sequence.
ACCESSION   DASZMB010000183 DASZMB010000000
VERSION     DASZMB010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3217715"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VG272_08440"
     CDS             complement(<1..225)
                     /locus_tag="VG272_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13747.1"
                     /translation="MQLNAYLLFKGNCEEAFKFYESALGGKIEAMLPFAGTPAEKHTA
                     PEFLQKIMHARLVVGDAVLMGSDAPGEKFDQ"
     gene            complement(316..732)
                     /locus_tag="VG272_08445"
     CDS             complement(316..732)
                     /locus_tag="VG272_08445"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWF13748.1"
                     /translation="MMIVKANQDSEAGKMPGEELLMAMGKYNEELVKAGVLVDLSGLQ
                     PSSKGFRVKFSGGKTTVIDGPFAETKELIAGYWIIKVNSREEAVAWAKKIPAPFGKDQ
                     EGTIEVRQFFEMEDFAPSEAIDLHTEVGKELAKQKK"
     gene            complement(986..1459)
                     /locus_tag="VG272_08450"
     CDS             complement(986..1459)
                     /locus_tag="VG272_08450"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWF13749.1"
                     /translation="MQKITPFLWFDGRAEEAANFYVSIFKNSKIGKVNRYGDHGPGPN
                     GAVMIATFHINGQDFIALNGGPQFKFTPAISFVANCENQQEVDELWEKLSESGEKRQC
                     GWLQDKFGISWQIVPTALPRLMSDPDREKTSRVMQALMQMTKIEIKKLEDAFHGK"
     gene            complement(1481..1843)
                     /locus_tag="VG272_08455"
     CDS             complement(1481..1843)
                     /locus_tag="VG272_08455"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWF13750.1"
                     /translation="MRFLSVYKTVETGLPPTPEEMAVMGKLIEEGMKAGWLLAVEGCL
                     PSSKGARVSRSKGNVVVTDGPFTETKELIGGLAILQADSREHAVELTKHFLKIAGDGE
                     CELRQLHEAGPMKNNTSR"
     gene            complement(1944..2300)
                     /locus_tag="VG272_08460"
     CDS             complement(1944..2300)
                     /locus_tag="VG272_08460"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HWF13751.1"
                     /translation="MKYMLLIYLDENGLSESERARCYQESAQFAVQLSNTGQYLAAGP
                     LHPTSTATSVRVRDNKRVVTDGPFAETREQLGGYFLIDARDLDEAIGIAEKVPAGRWG
                     TVEIRPVMEIAGLPTM"
     gene            2378..3640
                     /locus_tag="VG272_08465"
     CDS             2378..3640
                     /locus_tag="VG272_08465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWF13752.1"
                     /translation="MASQPSVTLAIEEVYRADWGRIVATLIRLFGDFDLAEECAQEAF
                     TAAVNQWSDGRIPESPRAWIIQTAKHKAIDRIRRRGLYAEKLESYVISGFVRAAEEPD
                     YDNTEIADDRLRLIFTCCHPALSPEAQIALTLRTLCGLETEEIARAFLVSPTTMAQRL
                     VRAKNKIREAGIPYVVPGTGDIAARQDAVLKVIYLVFNEGYVATSGASLVRTDLCVEA
                     IRLGRLVLALMAPQSPPEGKALLALMLLHDARREARLDDEGEIVILEDQDRSLWNREQ
                     IREALLLLKDTPRESRGPFALQAAIVAEHCKAEHPDDTDWAEIVQLYAQLERVQPSPI
                     VYLNRAVAVAMAEGPRAGLAMIERLAVSNDLDSYHLLHAARADLLRRLNSNVEAAKSY
                     ERALALVTNESERRFLERRLHEVQSTDE"
     gene            complement(3783..5705)
                     /locus_tag="VG272_08470"
     CDS             complement(3783..5705)
                     /locus_tag="VG272_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13753.1"
                     /translation="MIFLNRRSCIQFVFLALVIFVAPQARGQVDVLTQHNDNARSGAN
                     LKETKLNTSNVNKDRFGKLASRAVDGNVYAQPLVVSGAHVTGREAPTNIVIVATENNS
                     VYAFDADDGDQSSSQALLWHTGPDVLGEHIESRELYARIGAPACGDLTTKIGITGTPA
                     IQISKQGERKEGTIFVVAKSKSGSRYVYKLFALNLATGEKIAAVDIQGEAAGSGIGAG
                     GKGQNKVIAFDPMYELNRAALLLNGNTLYVAFGGHCDRGPYHGWVIAYDVSNPTTPKR
                     IAVFCSTPNGKGEDEEGRAGIWMSGEGPAADENGDIYFVTGDGTNNARTDFGDSVVKA
                     GVDGSGNFRVKDWYTPSNQEIMKNNDVDLGSGGTALIPNSHLLVTGGKEGRAYLIDRE
                     HMGRGDGASLQSFQVTHAPQPPNYYCLHGNPVVWTRADHMYVYFTGEEDPVKQYRLVP
                     AAGASEGWRFESDKPYRKSSDCPTAPQCVSAPFPNSLGDNSGPAREASVWMPGGFMSI
                     SANGSEEGSGILWVAMPYAENANHTVVRGVLRALDASDLSKPELWNSERTGDDEDQLG
                     LFAKFTPPTVANGKVYVATFEEETIGKDGMHRLAPHGRQATVAIYGLRCAAERCGEQS
                     LTSELKGKSINTEGAE"
BASE COUNT         1186 a         1812 c         1384 g         1379 t
ORIGIN      
        1 ctggtcaaac ttttcccccg gggcgtcgct ccccattaga accgcgtcgc ctaccacaag
       61 ccgcgcgtgc attatttttt gcagaaattc cggcgccgta tgtttttccg ccggcgttcc
      121 tgcaaacggc agcatcgctt ctatttttcc gcccagcgcg ctctcgtaga acttgaaagc
      181 ctcttcgcaa ttgcctttaa acagcaggta cgcattcagc tgcatgacga tctcctttca
      241 aagtgtgaaa tttaaaacca tttcccctgc actggataaa cgatcgagaa ttcccgaaat
      301 cgacaactat cccggttatt ttttctgctt ggccagttct ttccctactt ctgtatgcaa
      361 atcaatcgcc tcgctcggcg caaaatcttc catctcgaag aattggcgca cttcaatcgt
      421 gccttcctga tccttgccga acggagcagg gatcttcttg gcccacgcga ccgcctcttc
      481 ccgcgaattc actttgatga tccaataccc cgcgatcagc tccttggttt ctgcgaaagg
      541 cccgtcaatc accgtcgtct tgccgcccga gaatttcacc cgaaaacctt tcgagctcgg
      601 ctgcagtcct gacaggtcga ccagcactcc ggccttcacc agttcctcat tatatttccc
      661 catcgccatg agcaactctt ctcccggcat ttttccagct tcgctatcct gattcgcctt
      721 aacaatcatc ataaatcgca tatcgatctc ctctttttaa gtagcacagg cttcagcctg
      781 tggaatttcg gccttgcagg tacaaacccc acaggctgaa gcctgtgcta cttaaatccc
      841 ggtcccccaa ctcgtcttcc cgcttcttaa tctcaatatc ggtcagcctc atccgaccgg
      901 cctttatgtg tcataggctt tagcccgcgg aatttgggtc cttcacggac taagaaccca
      961 caggctgaag cctatgacac gaaattcact tcccgtgaaa cgcgtcttcc aacttcttga
     1021 tctcaatctt ggtcatctgc atcagcgcct gcattacccg gctcgttttt tcccggtccg
     1081 gatcgctcat caacctcggc aacgcggtcg gtacgatctg ccacgaaatt ccaaacttat
     1141 cttgcagcca cccgcactgc cgcttctctc cgctctcgga taatttttcc caaagctcat
     1201 ccacttcctg ctgattttcg caattcgcca caaacgaaat ggccggcgta aatttgaatt
     1261 gcggcccgcc attcagcgcg atgaaatcct gcccgttaat atgaaacgtg gcaatcatta
     1321 cggcgccatt cggtcccggt ccgtggtcgc cgtagcgatt caccttgcca atttttgaat
     1381 tcttgaaaat ggaaacataa aagttggccg cttcttcagc ccttccgtcg aaccacaaaa
     1441 atggagtaat tttttgcacg aaatcatcct tttttcgcaa ctatcgcgag gtgttgtttt
     1501 tcatgggccc ggcttcgtgc agctggcgca gttcgcattc accgtctccg gcaattttca
     1561 gaaaatgttt cgtcaattcg acggcgtgct ccctggaatc ggcttgcagg atcgcgagcc
     1621 cgccaatcaa ttcttttgtt tcggtgaagg gaccgtccgt cacgactaca ttgccctttg
     1681 accggctcac ccgggctcct ttcgagctgg gcaaacatcc ttccacggcg agcagccagc
     1741 cggccttcat gccttcttca atcaacttgc ccatcacggc catttcctct ggcgtcgggg
     1801 gaagccctgt ctctacggtt ttgtaaacgc ttagaaatcg caatgtaatt ctcctgaaat
     1861 tttatttttt gacctgattc gtttccatcc atcagtaagt cgaatggcgt tcggcaaaat
     1921 cgacaggcca cgcatcagct atttcacatt gttggcaatc ccgcaatttc catcaccggc
     1981 cgaatctcca ccgtccccca gcgtcccgcg ggaactttct cggcgatccc gatagcttcg
     2041 tcgaggtccc gggcatcgat caaaaaatag ccgcccagtt gttcgcgcgt ttcggcgaaa
     2101 ggcccgtcgg tcaccacgcg cttgttatcc cgcacccgca cgctggtcgc cgtggatgtg
     2161 ggatgcagcg gaccggcggc caggtactga cccgtgttgc tgagctgaac cgcgaactgc
     2221 gcggactcct ggtagcaccg tgcccgctcg ctctcgctga gcccgttctc atccaggtaa
     2281 atcagcagca tatatttcat ttccgcctcc ctgcgtagat tagtcgaatg gcattcccct
     2341 gatcgacatc tataccaaaa aattattaca atcggccatg gcttcccaac cgagcgtaac
     2401 ccttgctatt gaagaagttt accgtgcgga ctggggcaga atcgtcgcca ccctgattcg
     2461 tctgtttggc gattttgatc tcgccgagga atgtgcccag gaagccttta ccgcagccgt
     2521 gaaccagtgg agcgacggcc gaattcccga atcgccccgc gcatggatca tccaaaccgc
     2581 aaagcacaag gccatcgacc gcatccgccg ccggggcctg tacgctgaaa agctggagtc
     2641 ctacgtaatt tccggcttcg tgcgtgccgc tgaagagccg gattatgaca acaccgaaat
     2701 cgcggacgac cggctccgcc tgatttttac ctgctgccac cccgcgcttt cgcccgaagc
     2761 gcagatcgcc ctcaccttac gcaccttgtg cggcttggaa acggaggaaa tcgcgcgcgc
     2821 gtttctcgtg tcgcccacga ccatggcgca gcggctggtc cgcgccaaga ataaaattcg
     2881 cgaagccggc atcccctatg tcgttccggg taccggcgat atcgccgcgc ggcaagatgc
     2941 cgtcttaaaa gttatctatc tggtttttaa cgagggttat gtcgcgacga gtggggcgtc
     3001 tctggtcaga accgatctct gcgtggaagc gatccgactc ggtcgcttgg tcttagcctt
     3061 gatggcgccg caatctccgc cagaagggaa ggcattgctt gcgctcatgc tgctgcacga
     3121 cgcgcggcgc gaagcccggc tagacgacga gggtgaaatc gtaattttgg aagaccagga
     3181 tcgctctctg tggaatcgcg agcagatacg cgaagccttg ctcctgttaa aggatacgcc
     3241 gcgcgaatct cgcggaccct tcgctcttca agccgccatc gttgccgagc attgtaaggc
     3301 tgagcatccg gatgatacgg actgggccga gatcgttcag ctctatgccc aattggaacg
     3361 agtgcagccg tcgccaatcg tgtatttgaa tcgcgccgtg gccgtagcca tggccgaagg
     3421 tccgcgcgca ggtctcgcga tgatcgaacg ccttgccgtc tcgaacgatc tggactccta
     3481 ccacctgctg cacgccgcgc gcgccgacct gctgcgccgc ctgaattcca acgtggaagc
     3541 cgcaaaaagt tacgagcgtg cccttgcgct ggtcaccaat gaaagcgagc gaaggttcct
     3601 agagcgccgc ctccacgaag tgcaatccac tgacgagtag cacagccact cttggctgtg
     3661 tgcactttgc gaaactcaca caggcaagag tgcctgtgct actcaaacgt gcctcaaagg
     3721 atctttccgc agcctgttca gcagccgggg cacaaacttc aaattttcta tgcgctcacc
     3781 ttttactccg cgccctctgt gttaatgctt tttcccttca attcactggt gaggctctgc
     3841 tctccgcacc gctctgcagc gcaccgcagc ccatagatcg ccacggtcgc ctgtcgccca
     3901 tgaggcgcca gccgatgcat gccgtccttc ccgatcgtct cttcctcaaa cgtcgccacg
     3961 tacactttcc cattcgccac ggttggtgga gtgaattttg caaacagccc cagctgatcc
     4021 tcgtcatctc ccgtcctctc gctgttccaa agctccggct tcgacaaatc cgatgcgtcc
     4081 agagcgcgaa ggaccccgcg caccaccgta tgatttgcat tctcagcata gggcatcgcc
     4141 acccacagta tgccgcttcc ttcctcgctg ccattcgcgg agatggacat gaatccgccc
     4201 ggcatccaca cgcttgcttc tctcgcgggc ccggaattat cgcccaagga attgggaaac
     4261 ggagcggaca cgcactgcgg agccgtcgga caatcgctgc ttttccggta tggcttgtcg
     4321 gattcgaatc gccacccttc gctcgcgccc gcggcgggca ccagccggta ttgcttcacc
     4381 ggatcttctt ctcccgtgaa gtaaacgtac atatgatccg ctcgcgtcca cacaaccgga
     4441 tttccatgca agcagtaata gttgggtggc tgcggagcat gcgtaacctg aaaagattgc
     4501 agagaagccc cgtcgccgcg ccccatatgc tcgcggtcga tcagataggc ccgtccttcc
     4561 tttcctccag tcaccagcaa atgggaatta ggaatcagtg ccgttccgcc cgagcccaga
     4621 tccacgtcat tgttcttcat gatttcctga ttgcttggcg tgtaccaatc cttcacccga
     4681 aaattcccgg aaccatcgac gccggccttc acgacgctgt ctccgaagtc cgtccgggca
     4741 ttgttcgtgc cgtcacccgt cacaaaataa atgtcgccgt tctcatcggc ggcggggcct
     4801 tctccagaca tccagatgcc tgcgcgtcct tcctcgtctt cgcccttgcc atttggcgtg
     4861 ctgcagaaaa ctgctatcct ttttggagtt gtgggattgg agacatcgta ggcaattacc
     4921 cagccgtgat aaggcccccg gtcacagtga ccgccgaacg cgacatacaa tgtgtttcca
     4981 ttcagcaaca gagccgcgcg attcaactcg tacatcggat cgaaggcaat caccttgttt
     5041 tgacctttgc cacccgcgcc gatgcccgaa cccgcagctt caccttgaat atcaaccgcc
     5101 gcgatttttt ctcccgtcgc aagattcaac gcaaaaagct tgtacacgta gcggcttccg
     5161 cttttcgatt tagcgacaac aaagatagtt ccttccttac gctctccttg cttgctgatt
     5221 tggattgccg gcgttccggt aataccgatc ttcgtggtga ggtcgccgca ggcaggagcg
     5281 ccaatgcgcg catagagttc acggctttca atgtgctcgc ccaaaacgtc gggacctgtg
     5341 tgccagagca acgcctgaga ggacgattga tcgccatcat cggcatcgaa cgcgtacacg
     5401 ctgttattct cggtggccac aatcactata ttcgtaggcg cttcacggcc cgtgacgtgt
     5461 gccccggaaa ccaccagagg ctgcgcgtac acgttgccgt ccacggcgcg cgatgccagc
     5521 tttccaaaac gatctttgtt cacattcgat gtgttgagct tggtctcctt gaggttcgcg
     5581 ccggagcgcg cgttatcgtt atgctgcgtg agcacatcca cttgacctcg agcctgaggc
     5641 gcaacaaaaa tcaccagcgc tagaaaaacg aattggatgc agctgcgcct attcagaaaa
     5701 atcatgcagg aaaacccttt taaactcgga aggcgacagg ggactgggcc tatctagagt
     5761 t
//
LOCUS       DASZMB010000184         5334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_758977, whole genome shotgun sequence.
ACCESSION   DASZMB010000184 DASZMB010000000
VERSION     DASZMB010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758977"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="VG272_08475"
     CDS             complement(<1..1279)
                     /locus_tag="VG272_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HWF13754.1"
                     /translation="MTNDEHELAFATIEELSALLAKRKISPVELTQVFLQRIEQHNPA
                     LNAFLTVTSEHAFSAARRAEKLFLHPRSSRRDGSPLLGIPVTIKDNIWTRGIRSTAGS
                     KILRDFVPSEDSTAARKIGRAGAILLGKTNLNEFAYGITGSNAHYEPVHNPWALDRIS
                     GGSSSGSAVAIAAGLCVASVGTDTGGSIRVPSGFCGITGLKPTFGRVSVFGVQPLSPS
                     LDHVGPLARSVSDAAILLNVIAGRDSLDPTSANKKLEDFHDAFKKPLRKFRLGRPREY
                     YWEKLDPEVRSAADAAVSDMEKHGAVVREISLPHLKESLNAATDVSTAEIYHVHESAG
                     YFPARAADYGESVRNRIAAGANVLAHRFLAGLEVRKSLLVEFDAAFQEVDAIVAPTLP
                     IPAPPIGAEFMQIDGEQVGVRPLVVGHARPANFT"
     gene            complement(1279..3666)
                     /gene="lptD"
                     /locus_tag="VG272_08480"
     CDS             complement(1279..3666)
                     /gene="lptD"
                     /locus_tag="VG272_08480"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HWF13755.1"
                     /translation="MKLGRKFIVATRWRILFALAAYVFSMRITSAQEIQVPPPSTEAG
                     VRLEAEQQRKEGDLYFADGNVEIQYKNLRLHADHVQYNTKTYLATANGHVLLEVDTQH
                     LTAESADFNVVSGEGRFEHVHGEVMMEHRPNSNILVSPNPLVFEAEEVRRVNANTYTI
                     EHAWLTVCEPDKPNWKFFSPHATLHVDRKVALLNANFRLFRIPLFYFPYASVPAGRRI
                     RQSGFMIPEFADTSVKGVVLGDAYYWAPKDWTDATIGMAYLSRRGWQQNGEVRIKPWE
                     NVSMSARYFGVIDRGVATPILNAQGLPVTNSAGNVETQLQSQGGHSAQFQLDAQLHNG
                     WRAVADFNQLTSLIFQLAFAPTFGEAVNSEVRNAGFLTNNFRGFSVNFAATSYKNFVN
                     AQPQVSADVRSAPEARFTSVDQAPWKNLPFYFGVDAFAGAVHRDDENLVTDPATGVQT
                     VQPGINTRNFVERSEVAPRIVVPLRWGPWVGITSSFTTRTTIYGAQLVNGVLTNDSLR
                     RSTGEVNIDLRPATLERVWETKKRKWKHTIDPEIEYNYVEGVNQFNRFILFNEDETLT
                     NTNDFVYSITQHLFQRDGDGQAKDWASWRISQAYYFDPTFGGALVPGARNVFQALDSI
                     TPFAFADEPRRFSPINSDLIISPGGIYDAEIRLDYDTVRRRVTTAEALLKIRPTENFN
                     FTVAQFSIDNTAILAPYNAVLQPAANQIRAQMGYGSSNRRGWNAEAGASYDVKQSLLQ
                     NEFIEVSYNGSCCGISVEYRRLSLGEIRSENQFRLSLNIANIGTFGNLRRQEKIF"
     gene            4051..4833
                     /locus_tag="VG272_08485"
     CDS             4051..4833
                     /locus_tag="VG272_08485"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HWF13756.1"
                     /translation="MPAARQVATQTDGNLAIEPEWRREVSNKLRQYRARRNGASAELQ
                     TVLPFEPDPPQQIKTSDAFPEIFAPAPEKSVRDPRKHVSERFEISIPKLELGPNIENI
                     SADEPATVVEGLTNTDMYPVASLPERRLAALVDVGLLAFTYGGMLALFTVLGGHVGLN
                     KLDLTVAVATLGLFYAQYFALFTMFGGSTPGMMVQGLRVVSFRGETATSRQLAWRSLG
                     YLISAGSCFLGFIWAIWDDDQLCWQDRISHTYLTSNEGNTLT"
     gene            4957..>5334
                     /locus_tag="VG272_08490"
     CDS             4957..>5334
                     /locus_tag="VG272_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13757.1"
                     /translation="MSRLARISVVTLSVIVFLYVGLGYVLGKPSDDKSYRSLSVFGEV
                     MQRIQEDYVDEPNMAVVTAGAMHGLLESLDPQSGYLSPREYADYRDRMKNGVHGEIGM
                     TVSKRYGYIVIVSVVPDGPAEKAS"
BASE COUNT         1181 a         1399 c         1491 g         1263 t
ORIGIN      
        1 ccgtgaaatt tgccggccgc gcatgcccca cgaccaaggg acgcacgcca acttgttcgc
       61 catcgatctg catgaattca gcgccaatag gtggggccgg gatgggaagc gttggcgcga
      121 ctatggcgtc cacttcctgg aaggcggcgt cgaactccac gagcaaactc tttcgcacct
      181 caagtcctgc caggaagcga tgagccaaaa cgtttgcgcc tgccgcaatg cgatttctca
      241 cggactcgcc gtaatcggcg gctcgggccg gaaaatatcc agccgattcg tgaacatgat
      301 aaatttcggc ggtcgaaaca tcggtcgcgg cgtttagaga ttccttcaag tgagggagcg
      361 aaatttcgcg cacaacggcg ccatgctttt ccatatcact cacagccgcg tcggcagcgc
      421 tgcgcacttc aggatcgagc ttttcccaat aatattcgcg gggacggccc aaccgaaatt
      481 tacgaagagg ctttttaaaa gcgtcatgga aatcttccaa ttttttattt gcagatgtgg
      541 gatcgagcga gtctcgtcct gcaatcacgt tgagcaggat cgctgcatcc gaaacgcttc
      601 gggcgagcgg gccaacatga tcgagtgacg gcgaaagcgg ctgcacgccg aaaacgctga
      661 cgcgtccaaa cgtcggcttg agcccagtaa ttccgcagaa gccggaagga acacggatcg
      721 agccgcctgt atccgtgcca acggaagcca cgcacaagcc cgcggcaatt gccacagcgg
      781 agccggaact ggagcctcct gaaattcggt cgagcgccca cggattgtgc actggttcgt
      841 agtgcgcgtt gcttcccgtg atgccgtaag cgaattcgtt cagattagtt ttgcccagca
      901 ggatggcgcc cgcgcggccg atttttcgag cggcggtgga atcttccgat ggaacaaaat
      961 cccgcaaaat tttcgatccc gccgtggagc gaatgcctcg cgtccagata ttgtctttga
     1021 tggtgacggg tattccgagc aacggagacc catcacgccg cgaactgcgc gggtgtaaaa
     1081 agagtttttc cgcgcggcgg gcggcgctga atgcgtgctc agaagtaacc gtgagaaacg
     1141 cgttgagcgc gggattgtgc tgctcgatgc gctgcaaaaa aacttgtgtc agttccaccg
     1201 gtgaaatttt gcgcttcgcg agcaacgctg agagttcttc gattgtggca aaagctaatt
     1261 catgttcgtc gttcgtcatt agaatatttt ttcctggcgt cgtaggttgc caaacgttcc
     1321 gatattcgca atgttcagcg aaaggcggaa ttgattttca ctgcgtattt ctccgagcga
     1381 aaggcggcgg tattccactg aaattccgca acaggatccg ttgtagctta cttcgataaa
     1441 ttcattttgc aggaggctct gttttacgtc gtagctggcc ccggcctccg cattccagcc
     1501 tcggcggttg gacgaaccat atcccatctg ggcgcgaatt tgattcgcgg caggctgcaa
     1561 cacagcgtta tacggcgcaa gaatagccgt gttgtcgatg gaaaactgag ccaccgtaaa
     1621 attaaaattt tcggtcgggc gaattttcag cagtgcttcc gccgtggtga ctctgcggcg
     1681 caccgtgtca tagtccagcc ggatctccgc atcgtaaatt cctcccggcg aaataatcag
     1741 atcgctgttg atcggagaaa agcggcgcgg ctcgtccgca aaagcgaacg gggtgatgga
     1801 atcgagcgcc tgaaaaacat tgcgcgctcc agggacgagt gcgccgccga atgttggatc
     1861 gaagtaatat gcttgcgaaa ttctccaact ggcccagtcc ttggcctgcc catctccgtc
     1921 tcgctgaaat aagtgctggg tgatggagta gacgaaatca tttgtgttcg taagggtttc
     1981 gtcctcgttg aaaagtatga agcggttgaa ttggttcaca ccttccacat agttgtattc
     2041 gatttccggg tcgatggtgt gtttccattt tctttttttt gtttcccaca cgcgctccag
     2101 cgtggcaggc cgaagatcga tattcacttc gcctgtggac cggcgcagcg agtcattggt
     2161 taagacgcca ttcaccagct gcgcgccata aatggttgtg cgggttgtga agctcgaggt
     2221 gattccaacc cacggtcccc atctcagagg caccacaatg cgaggggcca cttccgagcg
     2281 ttccacaaag tttcgagtgt taattcctgg ttgaacggtt tgcactccag ttgccgggtc
     2341 tgtcaccaga ttctcgtcgt cgcggtgcac tgccccggca aatgcatcta ccccaaaata
     2401 gaacggcaaa tttttccacg gcgcctgatc cacggaagtg aagcgggctt ccggtgcgct
     2461 gcgcacgtcc gcgctcacct gtggctgggc gttgacgaag tttttgtagc tggtcgcggc
     2521 gaaattgacg ctgaatcccc gaaagttgtt ggtcagaaat ccggcgttgc gcacctcgga
     2581 attcactgct tcgccgaaag ttggtgcgaa cgccaattga aaaattaagg aggtaagctg
     2641 gttgaaatcc gcgaccgcgc gccagccatt gtgtagttgc gcgtcgagct gaaattgcgc
     2701 gctgtgtccg ccctggcttt gcagctgcgt ttccacgttg ccagccgagt tggtaaccgg
     2761 caatccctgc gcattcagga tgggcgtggc cactcctcga tcgatcacgc caaaatatct
     2821 ggccgacatc gaaacgtttt cccaaggctt tatgcgtacc tcgccatttt gctgccatcc
     2881 gcggcggctg agatatgcca tcccaatcgt cgcgtcggtc cagtctttag gcgcccagta
     2941 atacgcgtcg ccaaggacga ctcctttgac tgatgtgtca gcgaattccg ggatcatgaa
     3001 tccggattgt cgaattcttc gtccagcagg gacggaagcg taggggaaat aaaaaagcgg
     3061 aatgcggaag agacgaaagt tggcgttcag cagggcaacc ttgcggtcga cgtgcaatgt
     3121 ggcatgcgga ctaaaaaact tccagttggg tttgtccggt tcgcatacgg tcagccacgc
     3181 gtgttcgatc gtataggtat tcgcgttgac gcgccgtact tcttccgctt caaagacgag
     3241 cggattcgga gaaacaagga tgttggaatt cggcctatgc tccatcatga cttcgccgtg
     3301 gacatgctcg aagcgtcctt ctccactcac cacattgaaa tcggcggatt ccgctgtcag
     3361 atgttgcgtg tccacttcga gcaggacgtg tccattagcc gtagccagat aagtcttcgt
     3421 gttgtactgc acgtgatcgg cgtgcagccg aagattttta tattggattt cgacattgcc
     3481 gtccgcgaaa tagagatcgc cttctttgcg ctgctgctcg gcctcgagcc gcacgccagc
     3541 ttcggtggaa gggggcggga cttgaatctc ctgtgccgat gtgatgcgca tcgaaaaaac
     3601 atatgcggcc agcgcaaaga ggatgcgcca ccgggttgcg acaatgaatt tgcgaccgag
     3661 tttcatgatc ctgtgccgcc cgcgaactgt gacgaagggc gaaagaaatg cctgtaatag
     3721 ctcaaataaa aatagctcgt aagcctagca ttccccctga aggcttacaa tgattccggt
     3781 tgtctgcatc gaccactata gattgcgcgc aggggcaatg ggctgcctgg gttattcaat
     3841 tcgcgacaaa actctgtaaa tatctacgta aataatattg cggcgacgct tcgctggtag
     3901 aatagaaatg actcccgatt tccgttcggc gagacatccg cagcgcaaaa cggagcggtg
     3961 ataaaaacct gtcagtattg cggcgccgtg aacggcgaac cgaccgatat ttgttgcttc
     4021 tgtgacgccc ctctgggtgg tcgcggacgg ttgcctgctg cgcgccaagt tgccacccaa
     4081 accgacggta atctcgccat tgagcctgaa tggcgccgcg aagtctcgaa caaacttcgc
     4141 caatatcgtg cgcgtcgcaa tggtgcgtcc gccgaactgc aaactgtact tcccttcgag
     4201 ccggatccgc cgcagcaaat aaaaacaagt gatgcgtttc cggagatttt tgcacccgcg
     4261 cctgaaaaga gtgttcgcga tcctcgcaag cacgtcagcg agagattcga aatttcaatt
     4321 cccaaactgg aacttggccc gaacattgaa aatatttctg ccgatgaacc tgcaacagtc
     4381 gtcgaaggct taacgaatac tgacatgtac cccgtagcct cgttgcctga gcgacgattg
     4441 gccgccctgg tggatgtcgg cctgctcgct ttcacgtacg gagggatgct ggcactattc
     4501 acggttttgg gcgggcacgt cggattaaat aagctcgact taaccgtggc tgtagcaacg
     4561 ttgggacttt tctatgctca gtattttgct ctcttcacta tgtttggcgg atcgactccg
     4621 ggaatgatgg tgcagggttt gcgtgttgtg agttttagag gtgaaacggc gacctcgcgc
     4681 caactggcct ggcggagcct ggggtatttg atttccgcag gttcttgctt cctgggtttt
     4741 atttgggcta tttgggacga tgaccaactg tgttggcagg atcgaatttc tcacacttat
     4801 ctcacttcga atgaagggaa tacgctaacc tgaagtgcgg gggtgtaagc ccaccagcgt
     4861 cgttctggaa ttgacctaga aacgagaagc atgcgatacg cccgccgtgc tatcatgttt
     4921 gagatttaat cttcgtgaat gaggcctata acaaatatga gccgcctcgc cagaatatcc
     4981 gtcgtcacac tctccgtcat agtgttttta tatgttggac tgggctatgt gcttggaaaa
     5041 ccttcggacg ataagtctta ccgctcgctg agcgtgtttg gagaagtgat gcagcgcatc
     5101 caggaagact atgtggatga gcccaacatg gccgtggtca cggctggagc catgcacggc
     5161 cttctcgaat ctctcgaccc gcagtcgggc tacctgagcc cgcgtgaata tgccgattac
     5221 cgcgaccgga tgaaaaacgg tgtgcacggt gaaattggca tgactgtctc gaagcggtac
     5281 ggctacattg tgatcgtctc ggtggttccg gatggaccgg ccgaaaaggc aagt
//
LOCUS       DASZMB010000185        12713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_999087, whole genome shotgun sequence.
ACCESSION   DASZMB010000185 DASZMB010000000
VERSION     DASZMB010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 12713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12713
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999087"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="VG272_08495"
     CDS             complement(<1..865)
                     /locus_tag="VG272_08495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWF13758.1"
                     /translation="MQSNEPLQISVIVSCRNEIRHIRTFLGCLFHQDLRGLEMEVLIA
                     DGMSEDGTRSVLDEFDKKYSVLRVIDNPEKIAATGLNHAIWEAKGEIILRMDVHSLYA
                     PDYIRTCIAVLQETHADNVGGPALTRAQGYIPEAIAYAFHVPFAAGGAKFRDPDYEGP
                     VSTVPYGCWRKSTLNAVGCFDAELVRGQDDELNYRIVSAGGTVWQSPRIVSWYQPRTS
                     LSRLFRQFFQNGFWKVAAVRKHRRPASIRNLVPVSSLFAGILLLLCAEAASVARMHVL
                     QNASVAAFLIFS"
     gene            complement(928..2235)
                     /locus_tag="VG272_08500"
     CDS             complement(928..2235)
                     /locus_tag="VG272_08500"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWF13759.1"
                     /translation="MVLTPILYLEIGIATLIAVGVLWTRIEYGLFLYAFALGFPDVAL
                     PVGDAINVRVDDVLILMFLVRSLLWTAPSLSKNQKSIFKWQAVFFALCILSIAVESAL
                     GNPPGAYEPAKMAGCAVIFLVLPRLLQSERRFQFFISGLMCGGIALALQIRMHLGDNS
                     AANIANFQELKSAATFSTWNPNTIGQAAILLVFGAGLGAVLFSKSSMGKMVWAAVAIG
                     FALVPAMVFVRGTTLSIVVGIVAFLILSRQWKWVLAFATVCVCALLLIHARQPELMED
                     ATSVNVSTGEGFSHRFDRWGMAFRAIEAKPVIGQGFGQELPYLLLIGSEGRAHDAYLA
                     VWLELGMGGLLLFLIVIFQFTRAATILFRSVRFGSQGALILALILTLCLDSIALPTLY
                     WEKLPTIAMSLALALVGLCERDVSPEQLKEIQWNNFQQFAPRM"
     gene            complement(2270..2794)
                     /locus_tag="VG272_08505"
     CDS             complement(2270..2794)
                     /locus_tag="VG272_08505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HWF13760.1"
                     /translation="MRIIKYLWFHLVSLATGWLPDLRPVLRLRGFLMRPAFRSCGKNL
                     QVARRVNINFTNRVDIGNDVFLGLGCWLNAAAGIVLDDEAQVGPYAILITGDHTLQEG
                     SYRFGPRSFAPIRIGSGAWIAAHATVTKGVHVGRGALLAANSVATREVPAFTIAGGVP
                     AQPIQKKTRMGVVR"
     gene            complement(2804..4006)
                     /locus_tag="VG272_08510"
     CDS             complement(2804..4006)
                     /locus_tag="VG272_08510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF13761.1"
                     /translation="MPKTLGYFTPVRAYSSDGKIYMQSASGRVAEALAEQFEKVYLCA
                     RVVHGAPPGPFDSPLDAANIELIEQPYWNTTAESLLHFGGFTRAYFRTCARADVLFVR
                     GLCPYIAVLYLCAWIFQRPICHWIVGDSVALLRSAARKGRAADFLALLFARLDRWFTR
                     IGRWLTGGAFVCNGHALTRAYASPRTTEIVSSTIRNSEFFSRVDTCQHSVIHILFVGF
                     VRPEKGIEYLLHAVSQLDSCIAWRLEIVGRREYPDYFKKLREIVAARDLSRQICWTDY
                     VPNGAPLFNIMRSADILVLPTLSEGTPHVLVEARANGLPCISTWVGGVPDTVTHGYDA
                     LLVPSKDSRRLAQAIEQMISDGELRRKLICNGLAKARAQTLDQFITTVLKELDSDGSL
                     QNAPVPQD"
     gene            complement(4032..5345)
                     /locus_tag="VG272_08515"
     CDS             complement(4032..5345)
                     /locus_tag="VG272_08515"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HWF13762.1"
                     /translation="MIFSRANFGTFSTSVAIQACGAGTGILAARLLGPVARGELATVI
                     LWPTILSNLGLMGCSWVVAQEVAKDPQKESDWISVGTIVGFVMASLYFAAGFFLLPLL
                     LPADRIYLLPLARLCLLLLPIDIMNQVLLSAEHGRMRWRRYNFLRLSYFVFYLFFVGV
                     IGVTHRSRVGWFVAAFLASQLLSLAFRVWVQRKSLVSGKPTLRQCSYLIRESMPFFGA
                     TISNLISLQLDTILVVGVFNAEAAGVYAVASAFANGQSSLGEALGITSFAVLSNEANP
                     HERKKIITETFRQSTLISCGAGIVLACLIPIVVVPLFGIAFSKAVRPAILLALAASVL
                     GSANILNQGLRGAGRPLAGVVSQLIGAGVLVVSVLLFLRGFGLMGMAYAVGVSACVQL
                     LVLILAAAEWLEISPLAFWPFSAGNIRLFLQQVGGLRLRYSRNPA"
     gene            complement(5360..7687)
                     /locus_tag="VG272_08520"
     CDS             complement(5360..7687)
                     /locus_tag="VG272_08520"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HWF13763.1"
                     /translation="MGSDEKEPNKIEIFTPRDVRVDRRRVLPGGLRPSQMEKPPDLVA
                     WWRIIRKHRWTVITAFVVLFVTVLIGSFKEKPVYRAKALIEIDKEDPSVANPQELFQV
                     DEVSDAYLETQYKVLGSDDLAERVIHQLGLERNAEFLPSQIHWPWTSNVAHAASSAQA
                     GQNAGAADPFVEDTVLKRFQDRLDVKPVRRSRAVELRFDSRDPELAARTVNAVVDDYI
                     QKNFQARWDSAQKASIWLSQQLQDLKSKLEKSEDDLQKYSADNGLLYLETGKGDSESI
                     VNQTVVGIQGELTKAEADRFEKESIYRLLQAGDFGSLPGVFDNKLLQDLTIRLADLQR
                     EHAQLAATFTEEYPKVKEIQGQIIEIQGSLERERARAAQKISNDYFAAVRREKLVRQS
                     FAETQAEANQVAEKSVQYGMLSREVETNKGLYEGLLQRLKEASVSAGLKAGNIRVVDP
                     GRVPFKPIAPNYPLNLGLASVLGLGLGVGMAFLKEHLDQTIRYSEDVFNYLGVPVIGF
                     VPNYAALNKKPPKVITAAEKQVYFGAEERQDEVGGTFLDARSRNDLGIHKGSAFSEAF
                     RELRTSVILSSSGRFANSILVTSAQPGEGKTTVAVNLAFSLAQLGQPVLLVDADMRRP
                     TIQKYFPQRGSRLSSYLSGQGAWQEMVYPTSVRGLSIMHCGPIPENPSELLSSDRMRA
                     MLQESMATYRFVILDSPPLLNRADSRILGSMVSATILVVKGGETPRQVVQYAESQARS
                     VGANLIGVVLNNLDKSMNDYSYYSVPRPKDEAAEA"
     gene            complement(7691..8860)
                     /locus_tag="VG272_08525"
     CDS             complement(7691..8860)
                     /locus_tag="VG272_08525"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HWF13764.1"
                     /translation="MGRRAAKTKRNRNHVCAIFATASLVLFVVAVPAAAQSLRATVGD
                     STSNLSTQHAAAAKTLLQPEREDVLPSIAAGTADSRIGADDLLSITVFEAPEMNTVLR
                     VSASGDISMQLLGEVHALGLTPTELQGVLQGLLRSTYMRDPHVGVFIQELQSHAVSVV
                     GAVKMPGVFQIRGPKSLLEVLSMAQGLAEDAGDTVSVDRSGEQSEPDPSSSQVLKTGG
                     APGTFQRHELLTKSDAPESLLSDRNAEIENINLKSLLGASDSIRKVLIHPGDVVKVSR
                     AGIVYVVGEVNKPGGFVLQNNESISILQALALAQGTTHTSAIRETRIIRTDLATGNRT
                     EIAVNLGKIFSGKRPDILLEPKDVVFVPNSAAKSVFYRGSEMAIQTAAGVAIYKW"
     gene            complement(8946..9527)
                     /locus_tag="VG272_08530"
     CDS             complement(8946..9527)
                     /locus_tag="VG272_08530"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HWF13765.1"
                     /translation="MQLTEHLNETSFPGNAPDWYALFVRYQHEKFVALGLSNKNIETY
                     LPLCNSTRQWQDRAKQLWLPLFPSYVFVRENAHHQLQIISTPGVIHIVRFGGRPAIVS
                     QSQISAVRKILDGHYVVEPHPYLTAGDRVRVKTGPLAGVAGILTRKKNRAQLVISMEM
                     LGRSAAVEIELSNVERIGPKTSLLPKKHTTAVA"
     gene            9976..10692
                     /locus_tag="VG272_08535"
     CDS             9976..10692
                     /locus_tag="VG272_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF13766.1"
                     /translation="MPQLMNHPSRSHGENIRVLIADADRMSGQLIADDLTRGRHDIEV
                     VGVFNNSRETIRQIQEIRPDIALINAHLEDGKLTGYNILQSVRSLSPKTSAIMLIPES
                     ERELVVDAFRGGARGVFCRLQPIKFLPKCIRTVHEGQIWADTKNLIYLLDFLTQLKPL
                     RLIKPGGGMDRLTPREAEVVQLLTDGLSTREISQKLSLSEHTIRNYLSAIYDKLGVSS
                     RVELALYAVHREDLNRPQGQ"
     gene            11144..>12713
                     /locus_tag="VG272_08540"
     CDS             11144..>12713
                     /locus_tag="VG272_08540"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HWF13767.1"
                     /translation="MDDLISDFLVESNENLDRLDSELVKLETDPSSQELLSSIFRTIH
                     TIKGSCGFLGFSNLEKVAHVGESLLSRLREGKLSLSPEFTTGLLAMVDAIRDMLREIQ
                     KTGQDGNETYPKLIETLTKLQEQSQKPRDASIRAPALSQEIANKNQTNFMQDILSSAV
                     TRPVGTPGLDATARKDPGPSSPTVVADEEPPAVNAHAQEDTKASNAPGDTIRVDVHLL
                     DRLMNLVGELVLTRNQITQFSARQTDPNLVSPAQQLNLLTSELQEEVMKTRMQPISTI
                     FDKFPRVVRDVAMGCGKQVSIEMTGKDTELDKSLLEAIKDPLTHIVRNSVDHGIETPD
                     LRVARGKRAEGHLKLRACHEGGQVVIEISDDGAGIDTTRVKNKALERGLITPQQAVAM
                     SERELLNLIFLPGFSTAEKVTNLSGRGVGMDVVKTNIDRVNGSVDINSAMGAGTAIKI
                     KIPLTLAIVPALVVVCAGERFAIPQVSLCELVRIEADAANSKIEYVHGVPVYRLRGRL
                     LPLVYLDQALHLERT"
BASE COUNT         3099 a         3610 c         3256 g         2748 t
ORIGIN      
        1 cagaaaaaat gagaaaggct gcaaccgacg cgttttgtaa aacgtgcatg cgcgctacac
       61 ttgccgcctc ggcgcatagt agcaagagaa tccccgcgaa caaggaactc acgggcacca
      121 gatttcggat cgacgcaggc ctgcgatgct tcctcaccgc tgccactttc cagaatccat
      181 tttgaaaaaa ttgccgaaac agcctgctga ggctcgttct gggttgatac cacgaaacaa
      241 ttctgggaga ctgccacact gtgccgcccg ccgaaacaat gcggtaattc agctcatcgt
      301 cctggccgcg cacgagttcc gcgtcgaaac atcccaccgc gttcagggtg gatttgcgcc
      361 agcaaccgta aggcaccgtg ctcacgggcc cttcgtagtc tggatctctg aacttcgcgc
      421 cgcctgcggc aaacggtaca tgaaaagcgt acgcgatggc ctccggaata tatccctgtg
      481 cgcgagtcag cgccgggccg cccacgttgt cggcatgggt ttcctgaagc accgcgatgc
      541 acgtccggat ataatccggt gcataaagtg aatgcacgtc cattctcagg ataatttctc
      601 ccttggcctc ccagattgca tggttcaagc ccgtcgccgc gatcttttcc ggattgtcga
      661 tgacgcgcag cacggagtat tttttgtcaa attcgtcgag cacagagcgg gttccgtctt
      721 cgctcatgcc gtcggcgatt agcacttcca tctccaagcc gcgcaagtcc tggtgaaaca
      781 agcagcccag aaatgtccta atatgtcgga tttcattccg acaggagaca attacagaaa
      841 tctgaagagg ctcgttcgat tgcatacgtc atccgccttt ctcttcagcg gaacaatttc
      901 agacattcgt atttggacag ggagacgtta catgcgcggg gcgaactgct gaaaattgtt
      961 ccactgaatt tcttttaatt gctctggcga tacatctctt tcacacaacc cgacgagtgc
     1021 caacgcgaga gacatcgcaa tcgtcggaag cttttcccaa tacagcgtgg gcaaggcgat
     1081 actatcgaga caaagagtga gaatgagcgc caaaattaat gcgccctgcg aaccgaatcg
     1141 aacgctgcgg aagagaatag ttgcagcgcg agtaaactga aaaatgacta tcaagaacaa
     1201 gagcaatccg cccatgccca gttcgagcca aaccgcaaga taagcgtcat gcgctcgccc
     1261 ttcacttcca attagcagca ggtaaggcaa ttcttgtccg aaaccttggc cgatcaccgg
     1321 ctttgcttca atcgcccgga aggccatgcc ccagcgatca aaccggtggc tgaaaccttc
     1381 gcctgtggag acgttgacgg aagtggcgtc ttccatcaac tcaggttgcc gcgcatggat
     1441 aagcagcaac gcgcaaacgc aaaccgttgc gaatgccagc acccacttcc actggcgcga
     1501 aagaatcaga aacgccacaa tgccaaccac gatgctcagc gtcgtgccac gcacaaatac
     1561 catcgccgga accagcgcga acccaatggc gacagctgcc cacaccattt tgcccataga
     1621 tgatttggaa aacagtacag ctccaagccc tgcgccgaaa acgagtaaga tggccgcctg
     1681 cccgatcgta ttcggattcc acgtgctgaa agtcgcggcg ctttttagtt cctgaaaatt
     1741 cgcaatgttt gcggctgaat tatcacccag atgcatgcga atctgcagcg ccagggcaat
     1801 tccgccgcac atcaatccac tgataaaaaa ttgaaaccgc cgttccgatt gcagcagccg
     1861 cggcaggacc aaaaaaataa ccgcgcatcc cgccattttt gccggctcgt aagcgccagg
     1921 cggatttccc agcgcggact cgaccgcaat tgacaagata cataacgcaa agaacacagc
     1981 ctgccatttg aaaatgctct tctgattttt cgacagggat ggcgcggtcc aaagcaatga
     2041 tcgaacaaga aacatcaaaa tcaacacgtc atccacacga acattgatgg cgtctcctac
     2101 gggcaaagcc acatcgggaa acccgagcgc gaatgcgtac aggaacagcc cgtactcgat
     2161 tcgcgtccag aggaccccga ccgcgatcaa cgtcgcaatt cctatttcaa gatacaaaat
     2221 gggtgtaaga accatataaa cttggcgaaa atgagatgcg gtaatttttt cagcgcacca
     2281 ctcccatgcg tgtctttttt tgaatgggtt gcgcggggac cccgcctgcg atcgtgaatg
     2341 ccggaacttc gcgggtggct acggagtttg ccgcgaggag cgcgccgcgt cctacgtgaa
     2401 cccctttggt gaccgtcgcg tgtgcggcaa tccacgcgcc actgccaatg cgaatcggcg
     2461 caaaactccg cgggccgaaa cgataagatc cttcctgcaa agtatgatcg ccggtgatca
     2521 ggatcgcgta aggtcccact tgcgcttcat catccaatac gattccggcc gcggcattca
     2581 gccagcagcc caatcccaga aaaacatcgt tgccgatgtc cacgcgattt gtgaagttaa
     2641 tgttcacgcg ccgcgccact tgcagatttt ttccgcaact ccgaaacgca ggccgcatta
     2701 aaaacccgcg cagtcgaagc acaggccgca gatccggcag ccaaccggtc gccaagctca
     2761 caagatgaaa ccaaagatat ttgatgattc tcatatttct aacctaatcc tgcggaacgg
     2821 gagcattttg cagggaccca tccgagtcta attctttcaa aaccgtggtg ataaactggt
     2881 ccagcgtttg ggcgcgggcc tttgccaggc cattacaaat caacttccgc cgcaattcac
     2941 cgtcggaaat catctgttca atcgcttgag ccagccttcg cgaatctttt gaagggacga
     3001 gcagtgcgtc atagccgtgc gtgaccgtat ccggcacgcc tcccacccac gtcgatatgc
     3061 acggtagtcc gttggcgcgg gcttcgacca aaacgtgtgg agtgccctca gacagagtag
     3121 gcaataccaa tatgtccgcg gagcgcatga tattaaacag gggcgcgcca ttcgggacat
     3181 agtcagtcca gcaaatttgc cgactgaggt cacgcgccgc gacgatctcg cgcaatttct
     3241 tgaagtaatc gggatactcc cgccgcccaa caatttccag cctccatgca atacagctgt
     3301 ctagctgtga gaccgcgtga agcaagtatt cgattccctt ctccgggcgc acgaaaccaa
     3361 cgaatagaat gtggatcacc gagtgctggc acgtatcgac gcgtgaaaaa aactcgctgt
     3421 tcctgatcgt gctcgaaaca atttcggtag ttcgcggaga ggcatatgcc cgcgtcagcg
     3481 catggccgtt acagacgaat gctccgcccg ttaaccagcg gccgatacgt gtaaaccacc
     3541 tgtccaaccg agcgaagaga agtgcgagaa aatcggccgc gcggcccttt ctggctgctg
     3601 accgtaggag cgcaacggaa tcgcccacaa tccagtggca gatgggcctc tgaaaaatcc
     3661 atgcgcacag atagagcacg gcgatgtacg gacacaggcc gcgaacgaac aacacgtccg
     3721 cgcgggcaca cgtcctaaag taagcacgag taaacccgcc aaaatgcagc agcgattcgg
     3781 ccgtggtgtt ccaatatggt tgctcgatca gttcaatgtt tgccgcgtct agaggagaat
     3841 cgaaagggcc cggcggcgcg ccgtgcacaa ctctcgcgca taggtaaacc ttttcgaact
     3901 gctccgcaag cgcctcggcc acgcgaccgg atgccgactg catataaatt tttccgtcgc
     3961 ttgaatacgc gcgtacaggc gtaaaataac caagagtttt cggcatacat ttttaacgaa
     4021 acagctgact tttaagctgg gttgcgcgaa taccgcaaac gtaagccgcc cacttgttgc
     4081 aaaaaaagcc tgatatttcc agcgctaaag ggccaaaatg ccagcggtga tatctccagc
     4141 cactccgctg cggcgagaat cagaacgagc aattgcacac acgcgctgac gccgacggca
     4201 taggccattc ccatcaatcc gaagccgcgc agaaaaagca gtacgctcac gacaagaacc
     4261 ccggcaccaa tcaactggct cacgactccc gcaagcggtc gtcccgcgcc tcttaaccct
     4321 tgattcagga tattcgccga ccccaacacg gaagccgcca aagccagcag gatcgctggc
     4381 cgaacggcct tcgagaacgc tatgccaaac aaagggacga ccacaatcgg aatcaggcat
     4441 gccagaacaa ttcctgcgcc gcaggaaatc agcgtcgact gtcgaaatgt ttccgtgata
     4501 attttcttgc gttcgtgtgg atttgcttcg ttagacagca cggcgaaaga agtaattccc
     4561 aacgcttcac ctaaagagga ttgtccgttg gcaaaggctg aggccacggc gtaaactccc
     4621 gccgcttccg cgttgaacac gcccacgaca agaatcgtgt caagctgcag cgaaatgagg
     4681 ttactgatcg tcgcgccaaa gaatggcata ctttcgcgga ttaagtatga gcactgccgt
     4741 aacgtcggtt tgcctgacac aagagatttg cgctgcaccc atacccgaaa agccagggac
     4801 agcaattgac tagccagaaa tgccgccaca aaccagccga cccgggaacg atgcgtgaca
     4861 cctataacgc ctacaaaaaa caaataaaaa acgaaatacg aaagccgcag aaaattgtac
     4921 cgccgccagc gcatgcgtcc gtgctcggca gataaaagaa cctggttcat gatgtcgatc
     4981 ggcagcagca gcaaacaaag ccgagccagc ggaagcaaat agattcggtc agcgggcaaa
     5041 agtaagggaa gcagaaagaa tcccgccgcg aagtacaggg aagccataac gaatccaact
     5101 atggttccca cggatatcca atcgctttcc ttctgggggt ctttcgcaac ttcctgcgca
     5161 accacccagc tacaacccat taatccgaga ttactcaaaa tcgtcggcca caaaatcacc
     5221 gtagccaatt cgccgcgcgc cacagggcca agcaaccgcg ctgccagaat tcctgttccc
     5281 gcgccacacg cctggatcgc aacactcgtg gaaaacgttc cgaaattggc tctggaaaaa
     5341 atcataggtg tcgcaccgat tatgcttcgg ctgcttcgtc tttgggtcga ggcacggagt
     5401 agtaagagta atcgttcatc gatttatcaa ggttgttgag caccactccg attaaatttg
     5461 cgcctacaga ccgcgcctgg gattcggcgt attgcacaac ctgacgtggc gtctccccgc
     5521 ccttcaccac aagaattgtg gcgctcacca tgcttccgag gatgcggcta tcggcgcgat
     5581 tcagcagcgg cggcgagtca agaatcacaa atcgataagt tgccatggac tcctggagca
     5641 tggcgcgcat ccggtccgag gacaataatt ccgatgggtt ttccggaata gggccgcaat
     5701 gcataatgga taatccccgt acggaagtgg ggtacaccat ttcttgccat gctccttgtc
     5761 ctgacaaata actgctcagg cgagatccac gttgcgggaa atacttttga atcgttggac
     5821 ggcgcatgtc cgcgtcgacc agcaataccg gctggccaag ttgcgccaat gaaaacgcca
     5881 gattcacggc cactgttgtt ttcccctcgc ctggctgggc gctcgtcact aaaatggagt
     5941 tcgcgaaccg ccccgatgac gaaagaatga ccgatgtcct caactctcgg aatgcttccg
     6001 aaaatgccga gcctttatga atcccgaggt cgttgcgcga gcgagcatcc aagaacgtcc
     6061 cgcccacctc gtcctgccgt tcctcggcgc cgaaataaac ctgcttttcg gcagccgtga
     6121 tcacctttgg aggcttttta tttaaagcag cataattggg aacaaaaccg atgaccggaa
     6181 ctcccagata attaaatacg tcttcgctgt accgaatggt ttggtccaaa tgctctttaa
     6241 gaaaggccat gccgactccg agacccaggc caagcactga cgccagacct aaattgagag
     6301 gataatttgg agcgatcggc ttgaacggga ccctgcctgg gtcgacgacc cgaatattgc
     6361 ccgctttaag tccggcagaa acgctggctt ccttgagccg ctgcaataac ccttcgtaga
     6421 gtcccttatt cgtttcgact tcgcggctca acattccata ttgcacggat ttttcagcta
     6481 cctggttggc ctcggcttgc gtttccgcga aggattgccg cacaagtttt tcgcgccgta
     6541 ccgccgcgaa ataatcgttg ctgatttttt gcgccgcacg ggctcgttcc ctttccagcg
     6601 agccttgaat ttctataatc tggccctgaa tctctttcac ttttggatat tcttcagtga
     6661 aagtcgctgc cagttgcgcg tgctcgcgct gcaggtctgc gagtcggatc gtcaaatcct
     6721 gcagcaattt attgtcgaag acacccggta gagatccgaa atctccggcc tgtaacaggc
     6781 ggtagatgga ctctttttcg aagcggtcgg cctcggcttt ggtcagctca ccctgaattc
     6841 cgaccactgt ctgattcaca atactttccg agtcgccttt tcctgtttcg agatataaga
     6901 gaccgttgtc cgcggagtat ttctgcagat catcttccga tttttcgagt ttcgatttca
     6961 ggtcctgcaa ttgttgcgaa agccatatgg aggctttttg tgcggagtcc caacgcgcct
     7021 gaaaattttt ctgaatgtaa tcgtctacca cggcgttaac cgttcgcgcc gcaagctcgg
     7081 ggtctcgcga atcgaaacgg agttccacgg cccgactgcg gcgcaccggc ttcacatcga
     7141 gacgatcctg gaaccgtttg aggaccgtgt cctcaacaaa tgggtccgcc gcgcccgcgt
     7201 tctgccccgc ttgcgccgag gaagcagcgt gcgctacgtt cgaagtccat ggccagtgaa
     7261 tttgcgaagg caggaactcg gcgttgcgct ctagccccag ctgatgaatg acgcgctcgg
     7321 ctaaatcgtc gctgcccaga actttatatt gcgtttctag atacgcgtcg gagacctcgt
     7381 cgacctggaa caattcctgt gggttggcaa cgctaggatc ttccttatcg atttcaatca
     7441 acgccttggc ccggtacact ggtttttctt taaatgaccc gatcagcacc gtgacaaaca
     7501 gaacgacgaa tgcggtgatc accgtccaac ggtgttttcg aataattctc caccatgcga
     7561 caaggtccgg cggcttttcc atctgcgagg ggcgcagccc tcccggcaat actcgcctcc
     7621 tgtccacgcg gacgtcgcgc ggggtaaata tttcgatttt gtttggttcc ttctcgtcag
     7681 agcccatggg tcaccactta taaatcgcta ctcctgccgc ggtctgaatc gccatttcgg
     7741 aaccccgata gaacacgctt ttcgcggcgc tgtttggcac aaacacaacg tctttaggtt
     7801 ccagtaaaat atccggacgc ttcccggaaa aaatcttccc caggtttacg gcaatttctg
     7861 ttcggttgcc ggttgccagg tcggtgcgaa tgatccgcgt ttcgcggatg gccgatgtgt
     7921 gcgttgtccc ttgtgcgagg gcgagtgcct gcaggatgga aatactttcg ttgttctgca
     7981 aaacaaatcc gccgggttta ttgacctcgc ccaccacgta cacaattccg gcccgggaca
     8041 ctttcacgac gtctccggga tgaatcagca cctttcgtat ggaatcggaa gcccccagca
     8101 aacttttaag atttatgttt tcgatctcgg cgtttcgatc ggaaagtaac gattcaggcg
     8161 catcactttt agtgagcaat tcgtgccgct gaaaagtgcc gggtgcgccg ccggttttca
     8221 aaacctgaga acttgaagga tcgggttctg attgctcgcc actccgatct actgagacgg
     8281 tgtcccctgc atcctccgcc agcccttgag ccatggaaag aacttcgagc aacgacttcg
     8341 gtccgcggat ttgaaatacg cccggcattt ttacagcgcc caccacggag actgcgtgac
     8401 tttgcaactc ctgaatgaac acgccgacgt gcggatcccg catgtacgtg cttcgcagca
     8461 gtccttgtag gacgccctgc aactccgttg gcgtaaggcc tagggcatga acctcgccta
     8521 gcagttgcat ggaaatgtcg ccgctggctg agacccgcag cacggtgttc atttccggcg
     8581 cttcaaacac agtgatactg agcaggtcgt cagcgccaat gcgcgaatcg gcggtgccgg
     8641 cggcaatcga cgggagcacg tcctcgcgct ctggttgcaa taacgttttc gctgctgcag
     8701 cgtgctgagt actcaagttc gaggtactgt cgccaactgt ggcgcgaagg gactgcgcgg
     8761 ccgccgggac cgccacaaca aatagcacca gcgacgctgt ggcaaagatc gcgcatacgt
     8821 gattgcgatt ccgtttcgtc ttcgcggcgc ggcgacccaa cagaggaact gccagaccgc
     8881 gcgcgcacgt ttgcctgttt ctcttgcgtg tttcaccttt cgtagctagt tgatccctct
     8941 gcatgtcagg ccacagccgt cgtgtgcttt ttagggagta atgaagtttt gggcccaatt
     9001 cgttccacgt ttgagagttc aatttccaca gccgcggagc ggcctagcat ttccatcgaa
     9061 atcaccaact gagcgcgatt ctttttccgg gttaaaattc ccgctacccc cgccagtggc
     9121 ccagttttga cccgcactcg gtctcccgcg gtcaggtatg gatgaggttc aacaacgtag
     9181 tggccgtcca aaatcttcct gacagcgcta atctgagact gggacacgat ggccgggcgt
     9241 ccaccaaatc gcacaatgtg aatgaccccg ggagtggaaa taatctgcaa ctggtgatgg
     9301 gcgttttcgc gaacaaaaac gtagctgggg aacaggggca gccacaactg cttggcgcga
     9361 tcctgccact ggcgcgtaga attgcagagc ggcaggtacg tctcgatatt tttgttcgaa
     9421 aggccgagtg caacgaattt ttcgtgttgg taccgcacga acagcgcata ccagtccggc
     9481 gcgtttcccg gaaacgaggt ttcattgaga tgttcagtca gttgcatcaa gcttcgccct
     9541 ttgacttaca taaaatattt atctaaacgg atgcagccac ttttcttgct cgccgcacat
     9601 gcgaacgatc agaatcagct aaaagaaaaa aaccgtgctc gtgagcgagt ctgcggcgcc
     9661 aatgcaggtc gcttgcggac aagaccgaca gtctacgcct gcattccgcg cccaacgtga
     9721 cgcacacaat agacatctgc ccaagtttcg aataggggca atgcgcactg ccagagggca
     9781 cagggcatct cgttgtcatt attagtgaca acttgcccta ttggataaca gcaaggacca
     9841 gaaacattgg gacataccac gattccttgc cctgtggcac cccgcatgca cgtgtcatta
     9901 ttcatgacat ttctaacaca aaatgcttga ttcataaggc actttacgtt actgtgcgcg
     9961 ggttagcgca ttccagtgcc gcagttgatg aatcacccct cacgttcgca cggcgaaaac
    10021 atccgcgttc tgatcgcgga cgccgaccgt atgagcgggc agctgatcgc agatgacctg
    10081 acccgcggcc ggcacgacat tgaggtggtt ggggtattca ataattcccg cgaaaccata
    10141 cggcaaattc aagagatccg cccagatatt gccctgatca acgcgcatct cgaagatggc
    10201 aagcttaccg gatataacat tttgcaatcg gtgcgcagcc tgtcgcctaa aacatccgcg
    10261 atcatgctaa ttcccgaatc ggaacgagag ttggtggtcg acgcattccg aggtggggcc
    10321 cgaggcgtat tctgtcgcct gcagcccatc aaatttctgc ccaaatgcat tcgaaccgta
    10381 catgaaggcc aaatctgggc agatacaaaa aacctgatct atttactcga tttcctgaca
    10441 cagcttaagc cgctacgatt gataaaaccg ggtggcggca tggaccgcct gacgcctcgt
    10501 gaagcagagg tcgtacagtt attgacggac ggtctgagca cccgtgaaat cagccagaag
    10561 ctcagtctgt ctgagcacac catccgcaat tacctcagcg cgatttacga caaattggga
    10621 gtttccagcc gcgtcgaatt ggcgctttac gcagtccacc gcgaagacct taatcgcccg
    10681 cagggccaat aaaggctccg gccgcataac cttccgtcgg agttgcaaaa tcctgcccta
    10741 attttttgga ctttaagttt cttctaatgc catccgccac gatttataag agctttacta
    10801 tctcaagaaa tagaagtccg ctcaggtttt tgcgacccgg gagaatttaa gcgatcggtc
    10861 tagcttctgt ttttcatttt catccatgct aagtccctac cgattgtgag cttagccatt
    10921 agctgggctc tacgcgtggc ctcgtgccac tcatcgcttg ccaattcaac tttcggctaa
    10981 agtcacttca tcgagtggac gatgaactgc aagacagaag tgcgaacggt caattcagat
    11041 ataagacccg ttccaaagtg taggcaagct tgcgattgac tcagaaatac tccgcgcaat
    11101 cttaaaattt gcagtcatgg gctggcgccg actgggtggt gcgatggacg atttaattag
    11161 cgacttcttg gtcgaaagca atgagaacct ggaccgactg gatagcgagc tggtcaagct
    11221 ggagacggat ccgtcgtcgc aggaactctt atcgagcatc tttcgtacga ttcacaccat
    11281 aaaaggtagc tgcggttttc tcgggtttag caacctcgag aaagtggcgc atgtcggcga
    11341 gagcctactt tcacgactgc gcgaaggaaa gctttctctc tcgcccgaat tcacgacagg
    11401 cttgctggct atggtggacg ccattcgcga catgcttcgg gaaattcaaa aaacggggca
    11461 ggacggaaac gaaacttatc cgaagttgat tgaaacttta acgaagctac aggagcagag
    11521 tcaaaagccc agggacgcct ccattcgcgc accggctctg tcacaagaga ttgccaataa
    11581 gaatcagacc aattttatgc aagacattct ttccagcgcc gtcacgcggc ctgtgggtac
    11641 tcccggattg gacgcaaccg cccgcaagga tcccggacct tcgtccccca ctgtagtagc
    11701 tgatgaagaa ccgcccgcag tgaatgcgca cgcgcaggag gatacgaaag cttcgaatgc
    11761 acccggcgac acaattcgcg tggacgtgca tttactcgat cggctcatga atctggttgg
    11821 cgaattggtg ctgacgcgaa atcagatcac ccaattttcc gcacggcaaa ccgatccgaa
    11881 cctggtcagt cccgcgcagc agttaaatct tctcacaagc gaattgcagg aagaggtgat
    11941 gaagacgcgc atgcagccga tcagtaccat tttcgacaag tttccgcgcg tcgtacgcga
    12001 cgtcgccatg ggctgcggca agcaagtttc catcgaaatg acgggcaaag atacggagct
    12061 cgataagagc ctgctcgaag cgattaaaga tccgctgacc cacatcgtac gcaattccgt
    12121 ggatcacggg atcgagacgc cagacctgcg cgtggcgcgc ggcaagcgtg ccgagggaca
    12181 tttgaagctg cgcgcctgcc atgaaggcgg ccaggtggtt atcgaaattt cagacgacgg
    12241 agcgggaatc gacacaacgc gggtaaagaa caaagcactc gagcgcggcc tgatcactcc
    12301 ccagcaagcg gtagcgatga gtgagcgcga actgctgaat cttattttcc tgcccggatt
    12361 ttccacggcg gaaaaagtga ccaatttatc tggccgcggc gtggggatgg acgtcgtgaa
    12421 aacgaacatc gaccgcgtaa atggttcggt ggatatcaac agcgcaatgg gagcgggcac
    12481 ggccatcaaa atcaagattc cgctgacctt agccatcgtc cccgcgctgg tcgtagtgtg
    12541 cgccggggag cgctttgcaa ttccgcaggt aagcctctgt gaactggtca gaatcgaggc
    12601 ggacgcggca aactccaaaa ttgaatatgt gcacggagtt cccgtgtacc ggctgcgcgg
    12661 ccgtctgctt cctcttgtgt atctcgatca ggccctgcat ctggaacgca cgc
//
LOCUS       DASZMB010000186         7802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2941322, whole genome shotgun sequence.
ACCESSION   DASZMB010000186 DASZMB010000000
VERSION     DASZMB010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7802
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2941322"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..2384
                     /locus_tag="VG272_08545"
     CDS             234..2384
                     /locus_tag="VG272_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13768.1"
                     /translation="MKFLSLFVLFIALLGLASCGAAPVSTAITCTTSTSTVASNSSTN
                     TCTDPVTSISVTISPATVSVNVVTTQQFQDSIQGGTNNVTIWKVNGMTGGSDTLGRID
                     SNGLYHAPTTIPSPATVTVTAVSFEDQNVSATATVAITPAPAVAITSPSAPVTISAGT
                     ANVINFTASETGGSTDIILWYVGSVGGLGVLGGNSTYGTISASGVYTPPLTPPIGQTV
                     TVTAVAQDSPTSTATLAVTIAGYSLSSFQGRFTFSLSGNNASGHFFRAGSFIADGVGG
                     LNSVLEDVNTSTSATSTPIFTTGSYTVGSDGRGTLKFNDGLTPANFNFVLVNGTQLQI
                     IGVDSAETAIGQANAQDAGAFLNIPLAALSGTYIFDFAGVDGSKGLSQVGEFSADGAG
                     HVTNGLINVNDGGTPNQYQIDGTSTSCVSPASSLSSYSVSTNGRGTLTLTTCAGGPTL
                     TLNFYVVSRGSAKFIGTDTVKQVGGYTSTQDPNASFNAAALNGNYAFLLAGSAAGGPI
                     ATVGNFVLDGNGNITSGVLDENLNGVPSSLVFQTSGTNAGKYTVTPSGQGTLTFNTTG
                     RTYTLVFYLGEVGSGSTAVLQETDAGITSDGNFSFQQGAPFNLASVLGNYAMDTSGTS
                     GAALQVNTGQFGSNGAGTIVSGVIDSNTGGTTLALGQAATGSYSAPAATGRMTLTLTA
                     GALNYIGYIVSPSQIYILGIQPGELAAGALLRQF"
     gene            complement(2528..3268)
                     /gene="kdsB"
                     /locus_tag="VG272_08550"
     CDS             complement(2528..3268)
                     /gene="kdsB"
                     /locus_tag="VG272_08550"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HWF13769.1"
                     /translation="MAIVIIPSRYAATRLPGKPLVSLAGKPMIQRVWERASKATRVSR
                     VIIATDDERIIKAAAGFGAEAVMTRTEHRSGTERVAEVAVGIAGGGDEVFVNVQGDEP
                     LVEPESIDTLVEAIESDQGVSIATLMVPIAKPADIMDPNIVKVVFDFDDNAIYFSRAP
                     IPWVRDRESPVHVQHMKHLGLYAFRRAALLDFPTLPLGDLERIEQLEQLRWMENGYKI
                     RVAETHHDSVSVDVPADVARVEKLLSKS"
     gene            3524..4504
                     /locus_tag="VG272_08555"
     CDS             3524..4504
                     /locus_tag="VG272_08555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWF13770.1"
                     /translation="MIVIRKKLFFFLFAVAVLTVFSTAAFNQTQGPVGPPKESTEPAF
                     KPGQGIKVNVDMTLVNLTVTDPLDRLVTGLEKEHFRVYEDGVEQEVLTLSSEDVPVSI
                     GLVFDMSGSMSDKVEKAREAAVQFMRTANPRDQFFLVSFNDRAELTSGFTSSVDELQN
                     RMMFTASRGRTALLDAIYLGLSQMRGAHNGKRALLIISDGGDNHSRYNESDVKNYLKE
                     ADCQLYSIGIFDPVASRRTVEELEGPTLLSEMSEMTGGRVFPVGNLGELPDIAAKIGM
                     ELRNQYVLGYKSSNPQHNGQWRKIKVKLRPPKGLPPLNVYAKTGYYAANQ"
     gene            4542..5540
                     /locus_tag="VG272_08560"
     CDS             4542..5540
                     /locus_tag="VG272_08560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWF13771.1"
                     /translation="MISFAALFSSASYAQQQQQAPISPSVKPPAPPSPAPPPKEAPQD
                     TYKIRTQVSLVVLHASVLNDRGVFVPGLMEDNFRVLEDKVEQKLSVFKQEDVPVSFGL
                     VVDNSGSMRAKRPQVNAAALTFVKTSNSQDEGFVVNFNDDYYLDTEHDFTSDLDEMSK
                     ALERIDARGSTALYDAVIGSLDHLKKGTRDKKVLLVVTDGEDNASTHSLENAIERAQR
                     SDAVIYAVGVFSDDDIKHDHRAMKKARTALSELANATGGLAFFPETVDDTESICTQIA
                     RDVRNQYTLGYYSTNAAHDGTFRTVAVQVTPPHGMGKLTVRTRTGYYASRGPSSGN"
     gene            complement(5678..6859)
                     /locus_tag="VG272_08565"
     CDS             complement(5678..6859)
                     /locus_tag="VG272_08565"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF003766.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HWF13772.1"
                     /translation="MAPFERIIWIVLDSVGIGALPDAADYDDVGRDTLGHIAKSRPLA
                     LPNLVRLGLANIAPLAHLAPPAVPEGCYGKGATHSPGKDTTTGHWEMAGIWLPQAFPV
                     YKQGFPRELIEQFERAIGRKTIGNKPASGTEIIKELGEEHVRTGFPIVYTSGDSVFQI
                     ATHEDVIPIAELYRMCEIARKMLDGPHKVGRVIARPFTGTPGNYRRTERRHDYAVEPP
                     HPMLMDVLVEKKIPIFGIGKIHDIYNGRGVDRYITTKSNADGMQKLHATLAEQKSGLI
                     FTNLVDFDMLYGHRKDVDGFAKSLEEFDVLLAPLLGALAPRDLLIVTADHGCDPDPAW
                     PTTDHSREYVPIVTYSPTGKRGVNIGIRDTLADMGQTIAENFGTQILHGKSFLPEISS
                     L"
     gene            complement(7052..7759)
                     /locus_tag="VG272_08570"
     CDS             complement(7052..7759)
                     /locus_tag="VG272_08570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWF13773.1"
                     /translation="MASPSKTAPNRDAIHPFDRKYGTDTGGYLGPEDLANGCTNDAHN
                     YGYSAIAPSVFHEACRKWQETLPAVSGRIEAYTFVDVGTGKGRPLLLASELPFRKVIG
                     VELNDELARIAQQNVTRWVRIKKPKAKIRVVQEDATKFQWPRTPLLVYLYNPFACPLV
                     EQMAENIAAASPQGSGLADLLYVNPTCADRLAAQGLFTRLWTGRIPMDDADQKADPYG
                     TTSDLISIYRRIGGHTR"
BASE COUNT         1811 a         2161 c         2071 g         1759 t
ORIGIN      
        1 ccgacactgt tttatgtttt ggacacggac acaacaggca cggcgctcgg ggtcatcaac
       61 aaccagttct aaatgcgcca aattgcgtgc ctggcattaa agaatgaaaa cgcatggacc
      121 agcaacgcac tgaggctgga aatcttaact aaatggaatt tcacaaattg gggatcaggg
      181 ttgacgctga atcgaacgtg ttgggaattt caaattgcca agagagaggc gcagtgaaat
      241 tcctctcact gtttgttttg ttcatagccc ttttgggtct ggcttcctgc ggcgcggccc
      301 ccgtaagcac agcgattaca tgtacgacgt ccacgtcgac cgtcgcttcg aacagttcga
      361 caaacacctg cacggatcca gtgaccagca tcagcgtgac gatttcacca gcaactgttt
      421 ccgtaaacgt tgtgaccact cagcaattcc aggattcgat ccagggcggc acgaacaatg
      481 tgaccatctg gaaggtcaac ggcatgaccg gcggaagcga tacgcttggc cgaatcgatt
      541 ccaacggact ctaccacgcc cccactacga ttccttctcc ggccaccgtc acggtaacgg
      601 ccgtctcgtt tgaagaccag aatgtgtcgg ccacggccac ggtcgcgatt acgcccgctc
      661 cggccgtagc cattacttcg ccttccgcac ccgtgacgat aagtgccggc acggcaaacg
      721 taatcaattt cacggcaagc gaaacaggtg gatcaacaga tatcattttg tggtacgtgg
      781 gatccgtcgg cggcttggga gttttgggcg gaaattcgac ctatggcacg atcagcgcaa
      841 gcggcgtcta taccccgcca ctgacgcccc cgatcggtca aactgtcacc gtaacggcgg
      901 tggctcagga ttctccgact tccaccgcta ctctggcggt caccatcgct ggatattcct
      961 tatcgtcgtt tcagggacga tttacgtttt cactgtccgg caataatgct tcaggacatt
     1021 ttttccgggc cggcagtttc atagcggacg gagtaggcgg tttgaatagt gtcctcgaag
     1081 acgtgaacac atcgacgagc gctacatcca ctccgatttt tacgaccggg tcgtacaccg
     1141 ttggctcgga cgggcgcgga actttgaaat tcaatgacgg cctcacgcct gcgaatttta
     1201 actttgtgct cgtgaatggc acgcaattgc aaatcattgg tgtcgattcg gcggaaacgg
     1261 ctatcggcca ggcgaatgcg caggatgcgg gcgcattttt aaacattccg ctagcggctc
     1321 tcagcggaac ttacattttt gattttgcgg gagtcgacgg atcgaaaggt ctttcgcaag
     1381 ttggcgaatt cagcgccgat ggcgcaggcc acgtgacgaa cggcttaata aatgtcaatg
     1441 acggcggaac gccgaatcag tatcagatcg acggaacgag tacctcttgc gtctctcccg
     1501 cttcttccct ttcctcctac tcagttagca cgaatggacg cgggactttg accctgacga
     1561 cttgtgcggg cggtccgacg ctaactctta acttctacgt tgtttctcgg ggctcggcaa
     1621 aatttatcgg cacggacacc gtgaagcaag ttggcggtta tacttccacg caagatccga
     1681 acgcatcgtt caacgcagcc gcactgaacg gcaattatgc attcttactt gcaggatcag
     1741 ccgccggcgg gcccatcgca acagtgggaa attttgtgct ggatggcaac ggaaatatca
     1801 ccagcggcgt gctggacgaa aacttgaatg gggtgcccag ctccctggtg tttcaaacca
     1861 gcggtacaaa tgcaggaaaa tacaccgtaa ctcccagcgg tcagggcacc cttactttta
     1921 atacaacagg ccgcacctac acgttagtgt tttatcttgg cgaggtggga agcggttcaa
     1981 ccgctgtgct ccaggaaact gatgctggga ttacgagcga cggcaatttc agttttcagc
     2041 aaggcgctcc ctttaacctt gcatccgtgc tcgggaacta cgcgatggat accagtggca
     2101 cttccggcgc ggcgttgcaa gtcaacacag gccaatttgg atcaaatggc gcgggaacga
     2161 ttgtttcagg cgtgattgac tccaatacgg gtggaacaac tcttgcctta ggtcaagctg
     2221 ccaccggctc ttactccgcg ccagccgcga ccgggcgcat gacgctcacg ttgactgccg
     2281 gagctttaaa ttacatcggc tatatcgtca gcccctcgca aatttacatt ttgggaatcc
     2341 aacccggcga attggccgcc ggcgcacttc tcaggcagtt ctaataaatc cccgcaagtt
     2401 tttccgtgct ttgtgtagcc cgcctcttca gaagcggggg tttagacgac tcaacatcta
     2461 aatccccgcc tctgaagagg cgggctacag cagcatataa aaccccgcag gctgacgccc
     2521 gcgctaccta agatttactc agcagttttt ccactcgcgc cacatccgct ggcacatcca
     2581 cgcttacgga atcgtgatgc gtctcggcga cgcgaatttt gtaaccattc tccatccagc
     2641 gaagctgttc caactgctcg atgcgttcga gatctccgag cggaagcgtg ggaaaatcga
     2701 gcaacgctgc gcggcgaaac gcataaagcc ccagatgctt catatgctgc acatggactg
     2761 gtgattcgcg gtcacgcacc cacggaatcg gcgcgcgcga aaaataaatc gcgttatcgt
     2821 cgaaatcgaa caccactttt acaatgttcg ggtccatgat gtccgcaggc ttggcgatgg
     2881 gaaccatcaa cgtggcgatc gaaacgccct gatccgattc gatggcttcc accagagtat
     2941 cgatcgattc cggctcaacc agcggctcat caccctgcac gttcacaaag acttcatcgc
     3001 cgccgccggc aattccaacg gcaacttccg cgacacgctc agtgcccgac ctgtgttccg
     3061 ttcgggtcat tacggcttca gctccaaatc ctgcggcagc tttgataatg cgctcatcgt
     3121 ccgtggcaat aatcacgcga ctgacgcgcg tggctttgct ggcgcgttcc cacacgcgtt
     3181 gaatcatcgg ttttccggcg agagatacga gcggtttccc gggcagtcgg gtggcggcat
     3241 aacgagaagg aatgatgacg atagccatat tgttttttcc tcgcgccttt aaaaaattcg
     3301 gcgattctta aaaatcctat cacagtgttg gaagcgttag aaccgaaccg ataattacaa
     3361 aagggatcgg gaacattcgg cattctttct aatgtaaaac ttctgtcaaa atacagccgc
     3421 cggacttgca tcggatagaa tgaatggtag aatcccctca aggtttggag ttggaacccg
     3481 ccgtgccccc accccagcgc caacggcgaa ggcaggctac cgagtgatag ttatacgaaa
     3541 aaaacttttc tttttcctat tcgctgttgc tgtgctcacc gtgttttcaa ccgcggcatt
     3601 taatcaaacg caaggtcccg ttggtccacc gaaggaatcc accgaacccg cgtttaaacc
     3661 cgggcaaggc ataaaagtta acgtggacat gacccttgta aacctcacgg tcaccgaccc
     3721 gcttgaccgc cttgtcacag ggctagaaaa agaacatttc cgcgtatacg aagatggcgt
     3781 cgagcaggaa gtcctgacac tttcaagcga ggatgtccct gtctccatcg gcctggtatt
     3841 cgacatgagt ggcagcatgt ctgacaaggt ggaaaaagcg cgcgaggctg ccgtgcaatt
     3901 catgcgcacc gcgaacccac gggatcagtt ttttctggtc agcttcaacg accgcgcgga
     3961 attgaccagc ggattcacca gcagcgtgga tgaactgcaa aaccggatga tgttcacagc
     4021 gtcccgggga cgcactgcat tactcgatgc tatctatttg gggctgagtc agatgagggg
     4081 tgcacacaac ggcaagcggg ccttgctcat catatcggat ggaggggata atcatagtag
     4141 gtacaatgag agcgatgtga agaactattt gaaagaagcc gactgccaac tctactcaat
     4201 cggcatcttc gatccagtcg cttctcgccg gaccgtggaa gagttggaag gacctaccct
     4261 cctttcggag atgtcggaga tgaccggagg ccgggttttt cccgtaggaa atctcggcga
     4321 attgcccgac attgcagcca aaattggaat ggagcttcga aaccaatatg tccttggata
     4381 caaatcaagc aacccacagc acaatggcca atggcgaaaa atcaaagtca agcttcgtcc
     4441 acctaaggga ctccctcccc ttaatgttta tgccaagaca ggctattacg ccgcgaacca
     4501 gtagcggcag ccgattcagc gccattttaa gtatcttcct tttgatttca ttcgctgcgc
     4561 ttttttcttc agcctcttac gcgcagcaac agcagcaggc accgatttcc ccatccgtaa
     4621 aacctcccgc gccaccgagt ccggctccgc cgccaaagga agcgccgcag gacacttata
     4681 agatcaggac acaagtcagc ctagtcgtcc tgcatgcgtc cgtgttgaat gaccgaggcg
     4741 tgtttgttcc cgggctgatg gaagataact tccgcgtgct ggaagataaa gtcgaacaaa
     4801 aactttccgt cttcaaacag gaagacgtgc cggtcagctt cggcctggtt gtggacaaca
     4861 gcggcagcat gcgagccaag cgcccgcagg tgaatgcggc agcgctaaca tttgtcaaaa
     4921 cttccaattc gcaagatgag ggtttcgtcg tcaattttaa tgatgactat tacctggata
     4981 cggaacacga tttcacgagc gacctggacg aaatgagcaa agcgctggag cgtattgacg
     5041 cgcgcggtag cactgcgctc tatgacgcgg tgatcggttc gctcgaccac ttgaaaaaag
     5101 gcacccgcga caagaaagtt ttgcttgtcg tcaccgacgg cgaggacaac gccagcacgc
     5161 acagcctgga aaatgcgatt gagcgggcgc agcgttccga cgccgtgatt tacgcggtcg
     5221 gggttttcag cgatgacgat atcaagcacg atcaccgcgc gatgaagaag gcgcgcacgg
     5281 ctctgagtga attggccaac gccacaggcg gtttggcatt cttcccggaa actgtggatg
     5341 acacggaatc gatttgcaca cagatcgccc gcgacgtccg taaccagtac acgctgggct
     5401 attactccac caacgccgcg cacgacggaa cattccgcac cgtcgcggtt caagtcacgc
     5461 caccgcacgg catgggcaaa ctgaccgtgc gaacacgcac cggatactac gcgtcccgtg
     5521 gaccgtcctc cggcaattag tttgtagcgt atttgtagcg ccttttgtag ctcgcctctt
     5581 cagaggcgag ggtttagatg ttgcgttgtc taaaccccct cctctgaaga ggcgggctac
     5641 agcggcacgg ctcgccttcg cagaaatcaa aataaagtta caggcttgaa atttcgggca
     5701 ggaaactttt accgtggagg atttgtgtgc cgaaattttc cgcgatcgtt tgccccatgt
     5761 ctgccagggt atcgcgaatg ccaatgttca cgccgcgctt cccagtcgga ctgtatgtaa
     5821 cgatgggaac atattcgcgc gaatgatctg tggtgggcca tgccgggtcg ggatcgcagc
     5881 cgtgatccgc ggtgacgatc agcaagtcgc gcggcgccaa ggctcctaac agtggcgcga
     5941 gaagcacatc gaattcctcg agtgattttg caaatccgtc cacgtccttg cggtgtccgt
     6001 acaacatatc gaagtcgacg agattggtaa aaatcaggcc gctcttctgc tccgccaacg
     6061 tggcgtgcaa cttctgcatt ccgtccgcgt tgcttttcgt ggtgatatag cggtcgacgc
     6121 cacgcccatt atagatatcg tgaatcttgc caatcccaaa gattggaatt ttcttttcga
     6181 cgagtacatc catcagcatg gggtgtggcg gctcgacagc ataatcgtgg cggcgttccg
     6241 tacgccgata atttcctggc gtgcccgtga atggccgcgc aattacgcgc cccactttgt
     6301 gcggcccgtc gagcattttt cttgcaattt cgcacatgcg ataaagctca gcgatgggga
     6361 tcacatcttc gtgcgtagca atttgaaaca cgctgtcgcc cgaagtgtaa acaatgggaa
     6421 aaccggttcg aacgtgctct tcgcccaatt ccttgatgat ttccgtcccg gaggcgggtt
     6481 tattgccaat cgtcttgcgg ccgatggcgc gttcgaactg ttcgatcaat tcgcgtggga
     6541 aaccttgctt gtaaacgggg aacgcctggg gcaaccagat tccggccatc tcccaatgcc
     6601 ctgtcgtcgt atctttaccg ggagaatgag ttgcgccttt gccgtagcag ccttcaggaa
     6661 ccgcgggtgg cgcaaggtgg gctagcggag caatattcgc gagtccgagg cgcacaaggt
     6721 tcggcaaggc cagcggacgc gatttagcga tgtgtcccag agtatcgcgg ccaacgtcgt
     6781 cgtaatccgc cgcatcgggg agcgcgccga tccccacact gtccagcaca atccagatga
     6841 ttcgttcgaa cggagccaaa gtgggcctcg cagcaaagta tacagcctgc accatgttcg
     6901 ggaaacaaaa cggatgacta tgccacggtg aaaaccacag ccaaccaata ttgctgtagc
     6961 ccgcctcttc agaggcgggg gtttagacaa cgcgacatct aaaccctagc ctctgaattg
     7021 gcggattgca atttggagcc ccacaagcgt tttagcgagt gtgcccgcca attcgtcggt
     7081 aaatcgaaat taaatcggag gtcgttccgt aaggatcggc cttttggtcg gcatcgtcca
     7141 tgggaatgcg tccggtccaa agccgcgtga aaagtccttg cgcggctaat cgatccgcgc
     7201 aagtgggatt tacgtatagc aaatcggcaa ggcccgaacc ttggggtgat gccgccgcga
     7261 tgttttccgc catctgctcg acaagaggac acgcgaaggg gttatacagg taaacgagca
     7321 acggggttcg cggccattgg aattttgtcg cgtcttcctg gactacgcgg atttttgctt
     7381 ttggcttttt gatgcgaacc catcgcgtga cattttgttg tgcgatgcgc gcgagttcat
     7441 cgttgagttc cacgccaatc actttgcgaa atggcaactc cgatgccagc agcagcggcc
     7501 ggcctttacc cgtgccaaca tccacaaacg tgtaggcttc gattcttccc gatactgccg
     7561 gcaaagtttc ttgccatttt ctgcaagcct cgtgaaatac cgatggagca atggccgaat
     7621 agccgtagtt gtgcgcgtca ttcgtgcacc cgttggccag gtcttccggc ccgaggtagc
     7681 cacccgtgtc cgtaccgtat ttgcgatcga agggatgaat tgcgtcgcga ttcggagctg
     7741 ttttcgatgg tgatgccaaa atgattatcc tcgcactatt aacagtaacg cagaactccg
     7801 tc
//
LOCUS       DASZMB010000187         4250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4863651, whole genome shotgun sequence.
ACCESSION   DASZMB010000187 DASZMB010000000
VERSION     DASZMB010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4250
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4863651"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            245..1522
                     /locus_tag="VG272_08575"
     CDS             245..1522
                     /locus_tag="VG272_08575"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HWF13774.1"
                     /translation="MPVTNRWLMALAGVCLQMALGAAYAWSVFRIPLAKQFGWTISQI
                     TLTFLISWFFLGCAAVVGGLWMKRSQPRVVAMTAGLLWGGGVFLASFSANRLWWLYLT
                     YGVMGGIGLGMGYVVPIAVLVKWFPDRRGLITGIAVAGFGAGSLFSAPAAGWLMLRVG
                     LMPTFAYLGAVYAIIAISAGSFMKNPPAGWRPEGWTPSATQLSQRSTRDFTLGQALGT
                     WQWWAISGLMSINTMAGLSIVSQASPIFQELGNATVKTAAGLVGIISIGNGAGRIFWS
                     WVSDLTSRKMAFFAMYLVEVILFWSYHTIHSLPILGVATFILVMCYGGAYGITPAFAA
                     DYFGPRDVGSIFGLMMLPWAFAAAFGPYVFAYLRQINGNYAQALTFLASLMTAALILP
                     ILVRPPRSRNTENAADATQQFPVEALPTNTIDD"
     gene            1714..2772
                     /locus_tag="VG272_08580"
     CDS             1714..2772
                     /locus_tag="VG272_08580"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWF13775.1"
                     /translation="MSLNQDKLNEMLHRGINDFGATFHAALVTLGDKLGLYKGLAAGG
                     PQTPAELAKRSGTTERYVREWLAAQAAGGYVSLEPSTGRYYLSEEQAFTLADENSPAF
                     LPGAFQVALAATKAVPKLVEAFRTGEGMGWHEHDHELFHGTERFFRPGYAAHLVAEWI
                     PALTGVEARLKQGARVADVGCGHGASTVLMAQAYPKSTFVGFDYHEGSIQTARVRARE
                     AGVVDRVDFEVARAKDYPGSGYDLVTFFDCLHDMGDPVGAARHVLSTLSPEGSWMIVE
                     PFANDRVEDNLNPIGRVFYSASTLLCTPASLSQEVGLALGAQAGEKRTKDVVTSGGFT
                     RFRRATETPFNLIYEAQP"
     gene            2996..3448
                     /locus_tag="VG272_08585"
     CDS             2996..3448
                     /locus_tag="VG272_08585"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HWF13776.1"
                     /translation="MDPEENEPKLFSLTEAEQARRQVEPLLIDAMQGRREMGDLEESL
                     SAIANRIQIMGGVTIDYDTAARLRADLNQIVAKVKDALDQIQATGCIVKDLDSGLVDF
                     PSVIKNEEVYLCWRLGEDRIRFYHGQDEGFSGRKPIDPEDTGPRHPIQ"
     gene            3925..>4250
                     /locus_tag="VG272_08590"
     CDS             3925..>4250
                     /locus_tag="VG272_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13777.1"
                     /translation="MKSMKICIGFGLLAGVLSVAPAAFAQYPATFQVTKDGTTVMLED
                     YANPPLSSATHGGDTATEINYKGQLGRVTALRAEPANAPLAASRVFVDDQNGTLYIMD
                     TATKKFT"
BASE COUNT          909 a         1130 c         1215 g          996 t
ORIGIN      
        1 gtgaactgca tagccagccg gcggcgcaag gtcgcgcgtg ggaagactgg tgttcgcaag
       61 cgggaattgc tgatctgtca tcgaaataaa tgaaggtggt ttctgtggca taggcttcag
      121 cctgtggcac ttagtacttg caaggaccaa cccccacagg ctgaagccta tgccacaaaa
      181 aaatgcatag cccagctcta gaagttgtca tgatatcttg tgcgcagaag tcgatggagg
      241 aacgatgcca gtaacgaatc gatggttgat ggctcttgct ggcgtttgcc tgcaaatggc
      301 gctgggcgcg gcctacgcgt ggagcgtctt tcgcattcct ctagcgaagc agttcggctg
      361 gaccatttct caaatcacat tgacattttt aatcagctgg ttttttcttg ggtgcgccgc
      421 ggtcgtgggt ggattgtgga tgaagcgcag ccaaccgaga gttgtggcca tgacggcggg
      481 tttgctttgg ggcggaggag tttttctggc aagcttttcc gcgaacagat tgtggtggct
      541 ctatctcacg tatggtgtga tgggcggaat cggacttggc atgggctacg tggttccgat
      601 cgcggtgctg gtgaaatggt ttcccgaccg gcgcgggctc attaccggaa ttgcagtcgc
      661 gggattcggc gcaggttcgc ttttttccgc acctgccgca ggatggctga tgctgcgcgt
      721 tggcctgatg ccaacgttcg cctatctggg cgcagtgtac gcgatcatcg cgatctcggc
      781 aggctcgttc atgaaaaatc ctcccgcagg ctggaggccg gaaggctgga ctccctcggc
      841 tacgcaactt tcgcagcgct cgacgcgtga ttttacgcta ggccaggcgc tgggcacgtg
      901 gcagtggtgg gcgatcagcg gccttatgtc cattaacaca atggccgggt tgtcgatcgt
      961 ttcccaggcc tcgccaattt ttcaggagtt aggaaatgcc actgtcaaaa cggcggcggg
     1021 gcttgtagga atcatcagta tcggcaacgg cgcgggacga attttctggt cttgggtatc
     1081 cgatctgacg tcgagaaaaa tggccttttt tgcgatgtac ctggtcgagg tcattctgtt
     1141 ctggagttac cacacgattc attcgctccc gattctcggg gtggccacgt tcattctggt
     1201 gatgtgctac ggcggcgcgt atggcatcac gccggcattc gccgctgatt atttcggtcc
     1261 tcgcgacgtc ggctcgattt tcggattgat gatgctaccg tgggcattcg cggcagcttt
     1321 cgggccgtat gtttttgcgt atttgcgcca gatcaatgga aattacgcgc aagctctcac
     1381 ttttcttgca agcttgatga ccgccgcgct catccttccc atccttgtgc ggccgccgcg
     1441 tagccggaac acggagaacg ctgcggatgc aacacaacaa tttccagtgg aagcgttgcc
     1501 tacgaatacg atcgatgatt aatccgggtt gactctcgaa acttccattc cggattaaaa
     1561 aactgttcga tgtgaactgc tccatttaca ttaaccgctc gccgcgagta caatccgcga
     1621 taagtagccg gaatttcctg gccccatgca gggccacttc ggtcgttttt tatcccgacc
     1681 tggattgccc accttgagaa acggaggaat gcaatgtcac ttaaccagga caagctgaat
     1741 gaaatgctcc atcgcggcat aaatgatttt ggcgctactt tccacgccgc actcgtgacc
     1801 ctcggtgaca aactcggcct ctacaaaggg ctggccgccg gtgggccgca aactcctgct
     1861 gaacttgcca agcgctcggg gaccacggaa cgctatgttc gcgaatggct cgccgcgcaa
     1921 gccgcgggtg gatatgtttc gctcgaacct tcgaccggac gctactacct ctccgaggag
     1981 caggcgttta cgctggctga cgaaaatagt cccgcatttc ttcccggcgc ttttcaggtg
     2041 gctctcgccg caacaaaagc cgtgccaaaa ttagtcgagg cgtttcgtac cggcgaaggc
     2101 atgggttggc acgagcatga tcatgaattg tttcacggaa cggagagatt cttccgtccg
     2161 ggatacgccg cgcatttggt cgcggagtgg attcctgcgc ttacgggagt ggaggctcgt
     2221 cttaagcaag gcgcgcgcgt ggcggacgtt ggatgtgggc acggagcatc cactgtcttg
     2281 atggcacagg cgtatccgaa atccacattt gtaggtttcg attaccacga aggttcaatc
     2341 caaaccgcga gagtgcgcgc ccgagaagcc ggcgttgtgg atcgcgtgga ttttgaagtc
     2401 gctcgcgcca aggattatcc gggcagcggc tatgacctcg taacattttt cgactgcctg
     2461 catgacatgg gcgatccggt gggcgctgcg cgccatgtgc tgtctacgtt gtcgccggag
     2521 ggaagctgga tgatcgtcga gccgtttgcc aatgaccgcg tggaagacaa cctgaatccg
     2581 attggccgcg tgttctattc cgcttccact ttgctttgca caccggcatc gctatcccag
     2641 gaagttgggc tagctctcgg cgcgcaagct ggcgagaaac ggacgaagga tgtggtcacc
     2701 tccgggggat ttacgcggtt ccgccgcgcg acggaaaccc cgtttaacct gatttatgaa
     2761 gcgcaaccgt aacggccgct acgaaagctg ggtaccgctg ggttgtagcg cccacctttc
     2821 ttttcaggcg gccatccttt tccagatgtc ccacaagttg atccgctact gtcgggcttc
     2881 tttactggat ttgcgccatg caatgcacga caataggacc cggtcggact gtctcgcatc
     2941 taatcgataa aggacggaaa tccgcgcaac ttgcggattt tcacgcacta attttatgga
     3001 cccagaagag aacgaaccga aattattcag ccttacggaa gcggaacagg cgcgccgcca
     3061 ggtcgagccg ctgttgattg acgcgatgca agggcgccgc gaaatgggcg acctggaaga
     3121 aagcttgagc gccatcgcca accgcattca gattatgggt ggcgttacga ttgattacga
     3181 cactgcggca cgcctgcgcg cggatttgaa tcaaatcgtc gcgaaagtga aagacgctct
     3241 cgatcagatt caggcgaccg gatgcatcgt gaaagatctg gattcgggcc ttgtggattt
     3301 tccatccgta attaaaaatg aagaagttta tctgtgctgg cggctcggag aagaccgcat
     3361 tcgcttttac cacggccagg atgaaggttt ttccggacgc aagccaatcg atcccgagga
     3421 taccggtcct cgccacccca tccaataatt ttaaaaaatt gctgtgtggc cgcatccaac
     3481 gtgttctaga aagtttttcc atcagcgaaa aatccgaaag cggcccggtc gagccgccag
     3541 accgtatctc cgctctgccg tgcatcaaac cgtggattgg tatgaatggg gcgacgaaac
     3601 atttgccgcg cgtgcgccgg tctccgatcc tgccgcgctc ctcgcgctac tgactcaact
     3661 ccacctgagc ttggctcaat ttaaattaga ttcgcggaag tgaaagaaat tgcaaattcc
     3721 ttattctgca aaacgtgctt atcgccatga accgaaaccg ttctcttaga aaaatgaaaa
     3781 gcccgtggaa gggtccggtg ttagaatgcc gcccagtctc gaatcgggtt ggattgtttt
     3841 gcgtctctcg gcctgccgca ggccctgata tgcggaaaaa agaaaaagga gctgttcaca
     3901 atgtcgagta gcaagaactt tttgatgaag tcgatgaaaa tttgcatcgg atttggttta
     3961 ttggccggcg ttctctcggt cgcgcccgct gcattcgcgc aatatccggc gacattccag
     4021 gttaccaagg acggcacgac cgtgatgctg gaagactatg cgaatccgcc gctatcgagc
     4081 gccacgcatg gcggagacac tgccacggag atcaattaca aagggcaact aggacgcgtc
     4141 acggcattgc gcgccgagcc agccaacgcg ccgcttgccg cctcacgcgt ttttgtggac
     4201 gatcaaaacg gcacgctgta catcatggat actgcgacca aaaaatttac
//
LOCUS       DASZMB010000188         7217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4040795, whole genome shotgun sequence.
ACCESSION   DASZMB010000188 DASZMB010000000
VERSION     DASZMB010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7217
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4040795"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..425)
                     /locus_tag="VG272_08595"
     CDS             complement(183..425)
                     /locus_tag="VG272_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13778.1"
                     /translation="MTLNEIVEKYLAIAGGFSFLAPLADFGMQREEAERVFGAFDEDY
                     HISRYMHFMQVSGMAYKINGFEQTHVSLDEEIQFIL"
     gene            complement(430..1050)
                     /locus_tag="VG272_08600"
     CDS             complement(430..1050)
                     /locus_tag="VG272_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13779.1"
                     /translation="MARLSLQEAYPGISIRPRSRRLWARFMQWPAECIHMESAPHWMA
                     TYIPDMLYLRGKARSRREPRRPEISVCFDCLSGLLEPELAATPARVIAFEPDGESFTQ
                     YFFLGREDFSAAGLQSEVSAAIEARLANLGGACSDCGAPATWLWISRREVSSLDQADR
                     IATSPGALFCAKHGAAKLCEALGQIEDANLFYVNAPYGESGAYVWI"
     gene            1357..1818
                     /locus_tag="VG272_08605"
     CDS             1357..1818
                     /locus_tag="VG272_08605"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWF13780.1"
                     /translation="MAIAAGQTAPDFTLKDQSQKDVKLSDFAGKRNVVLVFYPLDFSP
                     LCTNEHACFVNDLKQFESLNAQVLGLSVDSVWTHKAFADKMGIPYPLLADFHPKGAVA
                     QLFGVYSQERGVSGRAVAIIDKDGKVAWFKNYEYPSIPDIKEVSEALAALK"
     gene            complement(1895..2350)
                     /locus_tag="VG272_08610"
     CDS             complement(1895..2350)
                     /locus_tag="VG272_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWF13781.1"
                     /translation="MGGFNKGTSKWLARATGACLVLGIFVSVGGRVRADDTPAPWVAP
                     DDAKKVKNPVPSTPESLAAAQDLFTDNCVLCHGEKGMGDGPGAKAIKVKPANFTDAKV
                     MAAETDGSLFWKMTNGRGPMPSWKDTLSDKERWELVDYIRKLGKDAAKK"
     gene            complement(2533..3714)
                     /locus_tag="VG272_08615"
     CDS             complement(2533..3714)
                     /locus_tag="VG272_08615"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HWF13782.1"
                     /translation="MSLGRLVRNFLLPAIVMMLMFVAPGRSQDTNYPEIAKLPNPYKL
                     VEGWPTLPASMNGGHWGEVIRVHLERNGNVWVFHRCFSVVPPGHATCVGRGEENPPIL
                     EFDPSGKLIRSFGDGLFAYPHGFTVADDGNLWATDVNDEPAILGLPAKNKDGVLMGQE
                     VLKISPKGEVLMTIGKEGVSGDGNDTFDRPTGVAVAPNGDIFVSDGHYPNVHHNARIM
                     KFSKDGKFIKSFGHLGAGDGEIDEPHDIAIGGTQNRVYVANRRNRRVEVFDQDGNFIT
                     SWKQFGQPSSVFVGKDDTIYVGASFWDAKDQIEVRAKARASENVKDYIELTKREKRGV
                     IIGSVKDGSLKAFIPDPADLTKLTDVGSSASGIAADDTGNTIYVADVAVHNLRKYVKA
                     Q"
     gene            complement(4088..5422)
                     /locus_tag="VG272_08620"
     CDS             complement(4088..5422)
                     /locus_tag="VG272_08620"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HWF13783.1"
                     /translation="MATSAADVSASTGAPSLPESYRSIPILANASFFRKMIAFAGPGY
                     LVAVGYMDPGNWATDLGGGSRYGYTLLSAVLISSLMAIFLQALSAKLGIATGRDLAQA
                     CREHYSPKATAVLWVFCEIAIAACDLAEVLGSAVALKLLFGLPLLVGVLLTGFDVLLV
                     LALEGRGFRVLETLVVTLIVTIGACFAYEIFFARPLWIEAARGFLPTWEIVKDRQMLY
                     LAIGILGATVMPHNLYLHSSIVQTRAFSLTAGGKREAIRFATLDSTIALGLSLFINAA
                     ILVLGAAAFHVRGMNDVADISQAYKLLTPVLGVSLASVLFAAALLCSGQNSTLTGTLA
                     GQICMEGFLHWKVSPWLRRMITRSMAIIPAALVIGIMGEKHTTSLLIFSQVVLSFQLP
                     FAVVPLIQFTSDRSKMGEFTNGAFTTIFGWFMAAAIIVLNAALLWLTFGGKL"
     gene            5717..6022
                     /locus_tag="VG272_08625"
     CDS             5717..6022
                     /locus_tag="VG272_08625"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HWF13784.1"
                     /translation="MAQPGSDQVQVQIKADEKELVGQFSNLVMFHHTAEEFTVHFIYV
                     FPNVPQGKLVSSVIVSPGHAKRILHALEENIRRYEKQFGPIRDVPEPTPVPNVGFVQ"
     gene            6331..6897
                     /locus_tag="VG272_08630"
     CDS             6331..6897
                     /locus_tag="VG272_08630"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWF13785.1"
                     /translation="MKAACSSCGAQHSLPDKQLAGLPRVQFQCGKCGKSTIVEIPASP
                     DATQVLTPLPGFARSAGAPWLAAGGGISEVSLQLPTGKAIALSVIAGPSRGLVYPIEK
                     PRVVLGRADADIIIQDKEISRWHCSIEIKGDVIRLRDMDSTNGTYFADERVRVAELKH
                     LSEFRIGTSVILLSVTPKLAPTHATKIT"
     gene            7035..>7217
                     /locus_tag="VG272_08635"
     CDS             7035..>7217
                     /locus_tag="VG272_08635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWF13786.1"
                     /translation="MPLLTATFISYNEEADLPRALASLKDIADEIIVVDSGSTDRTTE
                     IAQSFGASVFTRKLDSF"
BASE COUNT         1730 a         2121 c         1810 g         1556 t
ORIGIN      
        1 cgccctgccc atcctcctgg tcttcggcct aggctactat ctctggcgcc gctaccaata
       61 gaacaaaata gttcgggcga atgtttaagc agcacaggca ctcctgcctg tgcgggtttt
      121 gcaagtgcag catcccgcac ccctggggcg caaacaaatt cccggtacgt cagccaacaa
      181 acctacaaaa taaactgaat ctcttcatcc agcgaaacat gcgtttgctc aaatccgttg
      241 atcttgtatg ccatcccaga cacctgcatg aaatgcatat accggctgat gtgataatcc
      301 tcgtcaaacg ctccaaacac tcgctccgct tcctcgcgct gcatgccaaa atccgcgagc
      361 ggcgccagaa acgaaaatcc gcccgcaatc gccagatatt tttctacaat ttcgttcagc
      421 gtcataggct cagatccata cgtaggcgcc tgattctccg tacggcgcgt tcacgtaaaa
      481 taaattggcg tcctcaatct gccccaaagc ttcgcacaat tttgctgcac cgtgctttgc
      541 gcaaaaaaga gctccgggag aagtcgcaat tctgtccgcc tgatccaggc tggacacttc
      601 gcgccgcgaa atccacaacc acgtggcggg cgcgccacag tcgctgcacg cgcctccgag
      661 gtttgccagg cgcgcctcaa tcgctgcgct cacctcagac tgcaatcccg cggcagaaaa
      721 atcttcgcgc cccagaaaaa aatattgcgt aaaactttcg ccgtccggtt caaaggcaat
      781 cacgcgtgcc ggcgtggctg cgagttccgg ttcaagcaac ccgctgaggc aatcgaagca
      841 cacggaaatt tccggacgcc gcggttccct ccgcgacctc gccttgccgc gcaggtagag
      901 catgtctgga atgtaagtgg ccatccaatg cggtgcactt tccatgtgga tgcactccgc
      961 cggccattgc atgaaccgcg cccacagccg ccgcgatctc ggacgaattg aaattcccgg
     1021 ataagcttcc tgcaaactga gacgcgccat ctagcgccga tggtatcaga atccggcgca
     1081 gcgctcgacc aaatccgcta tcataaatcg ttgcccgatc gaatttgtcc ggcttcgcag
     1141 gggggattgc cctgctgtga cctgggtctt cagacctggg ggtttagggt aacgtcgagc
     1201 aagaactgaa ttagaggcaa tgcgcgcgcg ttagggtagt cgtagccgcc gtccacttac
     1261 cggcacacaa atgccccgct tctgaacagg caggctgcag cagtcggtta cagactccgc
     1321 attgcgaatg attggaaata acatttagga ggcttcatgg ccatcgccgc agggcaaacg
     1381 gctcccgact tcacattgaa agatcagtcg cagaaagatg tcaagctgtc cgattttgcg
     1441 ggcaagcgta acgttgtcct ggttttttac ccgctggatt tcagtccact ttgcacgaac
     1501 gagcacgcct gcttcgtcaa cgatctgaaa caatttgaat ccctcaacgc gcaagtcctg
     1561 ggcctgagcg tggacagcgt gtggacgcac aaggcctttg ccgacaagat gggcattccc
     1621 tacccgctcc tcgccgattt tcatccaaag ggcgccgtcg ctcaactttt tggcgtctat
     1681 tcacaggaaa ggggagtctc gggacgcgcc gttgccatca ttgataagga cggaaaagtt
     1741 gcatggttca aaaattacga atatccatcg attccggata tcaaggaagt atcagaggcc
     1801 ctcgcggcgc tcaagtagta accaaaaaaa ggtgcgtttg cgatctgccc ggcagaccgc
     1861 aaacgcacct ttgtcattct aatcccaaat aaatttactt cttggcggcg tctttcccca
     1921 acttgcggat gtagtccacc aattcccagc gttccttgtc ggacagagta tctttccagg
     1981 aaggcatggg accgcggccg ttggtcatct tccagaagag cgacccgtcc gtttcggctg
     2041 ccattacttt cgcatcggtg aagttcgcgg gcttaacctt gatagccttc gcgcccggtc
     2101 catctcccat acccttttcg ccatggcaca gcacgcagtt gtccgtgaac aaatcctgcg
     2161 cggcagccag ggattccggc gtggaaggca ctggattctt caccttcttc gcatcgtccg
     2221 gcgcgaccca tggcgcaggc gtatcatccg cgcgcacgcg tcctccgacg gatacgaata
     2281 ttccgagtac gaggcaggcg ccggtagcgc gcgccagcca cttcgaagtt cccttgttga
     2341 atccacccat tccgatgtct ccattagtcc gagatttgca aggctgtaaa aattctgcca
     2401 caacgcgggg cttatttcac ctgctaaacc tgcggcgtcc gagttgcggt cggcgtcaaa
     2461 cgaccggata cagtgccgag ccggcctgcc acgcgtaacg ccagcatttg tatgagtatg
     2521 tggccaataa acctattgtg ctttgacgta cttccgcaag ttgtgcacag ctacgtcagc
     2581 cacataaatc gtatttccag tgtcgtccgc tgcaattccg gacgctgacg accccacatc
     2641 ggtcagcttg gtcaggtcag ccggatccgg aatgaaagct ttcagtgagc cgtccttaac
     2701 gcttccgatg atgacgccgc gcttttcgcg cttcgtcagt tcgatgtaat ctttcacatt
     2761 ttcggaagcg cgagccttgg cgcgcacttc gatctgatct ttcgcatccc aaaacgatgc
     2821 gccgacataa atcgtgtcat ctttgcccac gaacaccgag ctgggctgcc caaactgttt
     2881 ccaggatgta ataaaattcc catcctgatc gaaaacctcg acgcgccggt ttctgcgatt
     2941 cgccacatat acgcggttct gcgtgccgcc gatggcgatg tcgtgtggct cgtcgatttc
     3001 tccgtcaccc gcgccgaggt gtccaaagga tttaataaac ttgccgtcct tggaaaactt
     3061 catgattcgg gcgttatgat gcacgttcgg gtaatgccca tccgaaacga agatgtctcc
     3121 gttgggcgcg accgccacac cggtgggacg atcaaacgtg tcgtttccgt ctccgctcac
     3181 gccctctttg ccaatcgtca tcaatacttc gcccttggga ctaatcttca aaacttcctg
     3241 gcccatcagc acgccatctt tgttctttgc aggcagtcct agaattgccg gctcgtcatt
     3301 gacgtctgtc gcccacagat ttccgtcatc ggcaaccgta aatccgtgag ggtacgcaaa
     3361 caagccgtcg ccaaaactcc ggatcaattt tccggaagga tcgaattcca gaatcggagg
     3421 attctcctca ccccggccta cgcatgtcgc atggcctgga ggaaccacgc tgaagcagcg
     3481 atgaaagacc cagacgttgc catttctttc aagatgcacg cgaatcactt cgccccaatg
     3541 cccgccgttc atgctcgcgg gaagagtcgg ccagccttct acaagcttgt agggattcgg
     3601 aagctttgcg atttcagggt agttcgtatc ctggctgcga cccggcgcga cgaacatcag
     3661 catcattacg attgcgggta aaagaaaatt cctgacgagg cggcccaacg acattttgat
     3721 gctcccttgt aaaaagcaaa cgcgacagct ttacgttttg atgtaagccc gcattttaac
     3781 ccgtgaggcc cgagcatgcc acagttctca taaaccggta gtggtgtcat ttgaaattta
     3841 gcagttgcac tcggatgaat gggatatgta atcaaatgac accagcaccc ataaaccgcg
     3901 aaaaaagaag ccgcccgccc aaaattaggc cggccgctac gaaaccaaag ttcgcatggt
     3961 ttcgtagcgt catctccgtt cccgctcttc gggacggaga tgacgactcg cgtctcaaac
     4021 ggacgcagag ccgaacaata tgttgaaatt aatgttgcag aaattactgg aagcttagaa
     4081 acgcagccta gagcttcccg ccaaacgtaa gccacagcag cgccgcattc agcacaatga
     4141 tggccgccgc cataaaccag ccaaaaatag tggtaaacgc tccattcgta aattcaccca
     4201 tcttggaacg atcactcgtg aattgaatca gaggcacaac cgcgaagggc agctgaaaac
     4261 tcaataccac ttggctgaaa atgagcaacg aagtcgtatg tttctcaccc ataattccga
     4321 tgaccaacgc cgcaggaatt atcgccatcg atcgcgtgat catccgccgc agccacgggc
     4381 tgactttcca gtgcaagaaa ccttccatgc aaatttgccc ggcgagcgtt ccggtgagcg
     4441 tggagttttg tccagagcac aggagagccg ccgcgaacaa aacactcgcc aggcttacgc
     4501 ccaatactgg agtcaacaat ttatacgcct ggctgatgtc cgctacgtcg ttcattccgc
     4561 gcacatgaaa cgccgcagct cccaacacca gaatggcggc gtttatgaat agcgacaatc
     4621 cgagagcgat cgtcgaatcg agcgtggcaa agcgaatcgc ttcgcgcttt cctcccgccg
     4681 tcaggctgaa ggcgcgcgtt tgcacgatgc tcgaatgcag atacaaattg tgcggcatca
     4741 ccgtcgcacc cagaattcca atcgccagat acaacatctg ccgatctttc acgatttccc
     4801 atgtgggaag aaatccgcgc gccgcttcga tccacagcgg gcgagcgaaa aatatttcat
     4861 acgcgaagca cgcaccgatc gtcacgataa gcgtgaccac cagcgtctcc aacacgcgaa
     4921 acccgcgccc ctccagggct agtacgagca gcacgtcaaa tccagtcaac aacacgccga
     4981 ccagcagcgg caagccgaac agaagcttga gcgccaccgc tgagccgagc acttccgcca
     5041 gatcgcatgc ggcgatggcg atctcgcaaa agacccagag aaccgccgtc gcttttggcg
     5101 agtagtgttc gcggcaggcc tgcgcgagat cgcggcccgt ggcgataccc agcttcgcgg
     5161 aaagcgcctg aagaaaaata gccatcagac tggagatcaa cacggcgctc agcagcgtgt
     5221 atccgtatcg cgaaccgccg cccaaatctg tggcccagtt tcccggatcc atgtagccga
     5281 ccgcgaccag gtatccgggt ccggcaaacg caatcatttt gcggaagaaa gaagcgttcg
     5341 caaggatggg gatactgcga taggactccg gcagtgaggg agcgcccgta ctcgctgaaa
     5401 cgtcagccgc ggaggtcgcc attgcctcgt ttggattgcc ttgtccctgt atcggatcga
     5461 aagaggctga tgaggatgga agcagcgaca tagttaacct agataaatat cttctataaa
     5521 ctagcattaa taaaatcgcc cgtcaatgca attgggaccc ggttgtgctt agaaattcat
     5581 cagttcggct gaagaaaagc cgtatttttt cgggcatttt taaaaagccc atgggctctg
     5641 cgaggcaacg gcagaatatg atagtgtcac cattggaacg attcgcgggg aacgatctct
     5701 gtgaacggag gaacccatgg cgcaaccggg tagcgatcag gtgcaggtgc agattaaggc
     5761 cgacgaaaaa gaattggtcg gacaattctc caatctggtg atgtttcacc acacggcaga
     5821 agaattcacg gtgcatttca tttatgtgtt tcccaacgtg ccgcaaggca agctggtttc
     5881 cagcgtgatc gtcagtccgg ggcacgccaa gcgcatcctg cacgcgcttg aggaaaatat
     5941 caggcgctac gaaaagcaat tcggacccat tcgcgacgtc cccgaaccta ctcccgtccc
     6001 caacgtcggt ttcgtacagt agaagaatcc ccgattgcgg tgatagcgca accgcagggg
     6061 cacggaatgc cgtgcccctg cggtcgatac gcaatattgc cgtacttcgc tgcaaattca
     6121 gcctagttcc catatcgaaa catcagattc gaagtcatct taaaccaaca aatctcatgt
     6181 taaattagct agtaggcgct acgcgtgagg aggccttttt cgctttccgg atcgcgatct
     6241 agccctagtg acgctttcga ttcgagatag caaatacctt ggccagccgt aactcacgcg
     6301 taagaatgga gttcgcttcc agaaaaattt atgaaagccg cttgctccag ctgtggtgca
     6361 caacattcgc ttccggacaa acagttggca ggtctgccgc gcgtgcaatt tcagtgtgga
     6421 aagtgcggga aaagcacgat cgttgaaatt ccagccagcc cggatgccac tcaagttctg
     6481 actcctttgc ccggatttgc gcgtagcgcc ggggcgcctt ggctggcggc aggcggcggc
     6541 ataagcgagg tcagtcttca actgcccacg ggaaaagcca ttgccctttc cgtgatcgcc
     6601 ggaccatccc gcggccttgt atacccaatc gaaaaacctc gcgtggtgtt gggcagagcc
     6661 gacgcagaca tcatcatcca agacaaagaa atctcgcgtt ggcattgctc gatcgaaata
     6721 aaaggcgacg tcatccgttt gcgggatatg gattcgacca acggcacata tttcgccgat
     6781 gaacgcgttc gcgtggcgga attaaaacat ctctccgagt tccgcatcgg aacttccgta
     6841 attctcttga gcgttactcc aaagctagct cccacccacg ccacaaaaat aacttgagcc
     6901 ttaagtggcg cgggcttcag ccgcagaatt tagggcttgc aaggactatc ccccgcaggc
     6961 aaaagcccga gccgcttctg cagaactgct cacacccgct gcatttcgtg ctaccgtaac
     7021 cgattggatt tccaatgcct cttctgactg ccacattcat ctcttacaac gaagaagcgg
     7081 acttgccgcg cgccctcgct tccctgaagg atatcgccga cgaaattatc gtggtggatt
     7141 caggctccac ggaccgaacc accgaaattg cgcaatcctt cggcgccagc gtcttcacgc
     7201 gcaaactgga cagcttt
//
LOCUS       DASZMB010000189         7425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3220461, whole genome shotgun sequence.
ACCESSION   DASZMB010000189 DASZMB010000000
VERSION     DASZMB010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7425
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3220461"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..1612)
                     /locus_tag="VG272_08640"
     CDS             complement(251..1612)
                     /locus_tag="VG272_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HWF13787.1"
                     /translation="MSNLQPTSTPANLAYVMYTSGSTGTPKGVMIVQSGLVNYLTWAI
                     KTYSVQTGGSIPVHTSTSFDLTVTGLYPPLLVGGRVEILPEDVGGQSLVKALKKGRDR
                     SLVKITPAHLELLNQQIRPEEAAGSTKVFVIGGENLVAESLGLWRKSAPATRLINEYG
                     PTETVVGCCTYEVAANDPATGSVPIGQPIANTQLYILDRYKNAVPVGVTGELYIGGAG
                     VARGYLNRPELNKERFIADPFSGQIGSRLYKTGDLARYRKDGILEYLGRVDNQVKVRG
                     YRIELGEIEATLASDPRVKSCAVLVREDEPGNKQLVGYVVPREGKAPTAEDLQQFVKQ
                     KLPEYMAPSQFVFLDSIPLTTNGKVDRKALPAPTYGNVSEGKDFAAPRSETEKAIADI
                     WSKLMKLERIGIHDDFFDFGGHSLMAMKMVSQIEERFGISLPLADFLEEPTIAGLAKK
                     VRP"
     gene            complement(1649..2890)
                     /locus_tag="VG272_08645"
     CDS             complement(1649..2890)
                     /locus_tag="VG272_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HWF13788.1"
                     /translation="MMRRSEQNHEGRDGIIAMNIQEDLLLEQISYWKEELAGAPTNLE
                     LPTDQPRPAMQSLRGATETFELPQDLLRPLEDVGRQEQSTLFMILASGFAAMLHRYTG
                     QDDILIGTPVLGRVPGETENSRNRLQNTAVLRSKFSEHLNFRGLLQQVRERTMAAHAH
                     ADVPFDQLVAELAPERDSSHAPVVQVLFAFHTAAEAAQAPKGSDKSLVNAASSGYDLA
                     LILSETANGLSGKIEYSTDIFAAQSIQRMCGHYTTILTSIALNPHQCISELPMLTERE
                     RIQILYDWNSTRAEFPQTCVHQLFEQQVAQHSDSVAVVFEDRQLTYKELNQRANQVAN
                     FLRKRGVGPDVLVGVCLERCPEVLVALLGIWKAGGAYVPLDPAYPADRLSFMVKDSAA
                     KVLLTDSKHKPLFASAAGVTF"
     gene            3645..5357
                     /gene="asnB"
                     /locus_tag="VG272_08650"
     CDS             3645..5357
                     /gene="asnB"
                     /locus_tag="VG272_08650"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF006949.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HWF13789.1"
                     /translation="MCGILAILNLNPVRPINTELRTQALTMVKKIRHRGPDWSGTFSD
                     EQAIICHERLSIVDVEHGAQPLIDRQKGTVLAVNGEIYNHKDLRDQLKQPHEFQTASD
                     CEVLLYLYDEEHPKKFLNRVNGIFAFVLYDPKKKTFLIARDPIGVNPLYVGWDRHENL
                     YVASEMKSLAGVCERILEFPPGHYYLGNEADKGFQKYYEPKWAEPGFCPSEPYDPARL
                     RTALESAVHRQLMSDVPYGVLISGGIDSSIVAAVAAKFSARRVDENDLSPAWWPRLHS
                     FAIGLEGAPDLLPAKTVADHIGSVHHEIHFTVQEGLDALSDVIYQLESFDITSIRATT
                     PMYLLMRKIRTMGIKMVLSGEGADEIFGGYLYFHKAPNGRELHEETVRKLQKLHLYDC
                     ARANKASSAWGVEARVPFLDREFLDVAMVLDPDVKLPKNAKHTRPIEKWPLRKAFEGY
                     IPDSVLWRQKEQFSDGVGYSWINGLKATAEHEITDGMMRGAAERFPVKTPQTKEAYFY
                     RQIFETHFPWATAINCVPWERSVACSTETALKWDAAFQNNVDPSGRAVVDVHADAYEV
                     PTKV"
     gene            5520..6665
                     /locus_tag="VG272_08655"
     CDS             5520..6665
                     /locus_tag="VG272_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWF13790.1"
                     /translation="MPNYESPWMNEELRMFRKALREFIQEEFVPKQSKWREQHRPDVE
                     AWKQAGATGLLLADIPEEYGGGGGTFAHNAVVTEELAQAGVNFGTGVQSIVSHYILQY
                     GNEEQKKKWLPAMAQGELIGAIAMTEPSAGSDLQAIKTAARLEGNEYVISGSKTFITN
                     GGLANLVCVAAKTNPKVAGFRGISMIVVETKNLKGYRVGKSLEKVGMHEIDTCELFFD
                     DVRVPSTNLLGKGEGAGFAQMMDQLRYERLMIGVGAIASIERALDLTIQYAKERKAFG
                     QTLMEFQNARFKLAQCKTEAQIGRVFIDNCVMRFLTGQLDPVTAAMAKYWTTECQCRI
                     VDECVQLFGGYGYMSEYPIARMWMDGRVQRIYAGTNEILEELIGWSL"
     gene            complement(6696..>7425)
                     /locus_tag="VG272_08660"
     CDS             complement(6696..>7425)
                     /locus_tag="VG272_08660"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF13791.1"
                     /translation="QNIDLEIEQGEFVCIVGPSGCGKSTLLHLIAGLDKPTTGQIIVD
                     GGPVKGPGTDRILLFQELGLFPWLTVRQNVEFGLKMAGVSKAERKERARIFLRMVHLS
                     HFEDHYIHQLSGGMKQRVALARSLALRPKILLMDEPFAALDAQTRDMLHDELERIWKE
                     TSPTIVFVTHNVREAVRLGDRVLLMSFRPGRIKAQFEVVLDRPRHVEDPDVAQLSKEI
                     LGQLREEIDRSFNAEYSGKKNVSD"
BASE COUNT         1742 a         2031 c         1976 g         1676 t
ORIGIN      
        1 agtcccgacg acgagcttgg tttgatcggg gttttaaccc ctgaagcttc aggggctgaa
       61 gcccgcgacg aaaggcaacc caaatggccg agttgaaact cggccctcct aaattcgaga
      121 aattttcaaa ttaggacact accattggct tgttaggcca tccatttgaa gtgcgcgcgc
      181 agaaaggctg ctcgcccgaa aagaagggct ggcgttacaa aacgcagatt caggatgtgt
      241 tgaggtaaac ctagggacga actttctttg ccagacccgc gatcgtcggt tcttccagga
      301 agtccgcgag cggcaaactt attccgaatc tttcctcgat ctgcgacacc atcttcatcg
      361 ccatcaatga gtgtccgccg aagtcgaaga aatcgtcgtg aattccgatt cgctccaatt
      421 tcattaattt agaccagatg tccgcgattg ctttctcggt ttcggagcgc ggagcggcga
      481 aatccttgcc ctctgaaaca tttccatacg ttggagccgg cagagccttg cggtccacct
      541 ttccattcgt ggtcagagga atggaatcaa gaaaaacaaa ctgagagggc gccatatatt
      601 ccggcaactt ttgtttcacg aattgttgca ggtcttcggc cgtcggcgct ttgccttcgc
      661 gtggcactac atagccgacc aattgtttat ttcccggctc atcttcccgc accagaaccg
      721 cgcaggattt tacccgtgga tcgctggcaa gggtcgcttc gatctcgccc aattcgatgc
      781 ggtacccgcg cactttcacc tggttgtcta cgcgacccag gtattcgagt attccatctt
      841 tgcggtaacg cgccagatct cccgttttat acaggcgcga accaatctgc ccggaaaatg
      901 gatcggcaat aaatctttct ttgttcagtt ccggcctgtt caggtacccg cgcgccacgc
      961 cagccccacc gatgtacaac tcgccagtca caccgaccgg cacggcgttc ttatagcgat
     1021 cgagaatgta gagttgtgta ttggcgattg gctggccaat gggcacggag ccggttgccg
     1081 gatcgttcgc ggcaacttca tacgtgcagc agcccaccac ggtttccgta ggcccgtact
     1141 cgttgatcaa acgcgttgca ggagcggact ttctccacag gccgaggctc tccgccacca
     1201 gattttcgcc accaatgacg aacaccttgg tgctgcctgc tgcctcttcc ggacgtattt
     1261 gctgattcaa aagttccagg tgagccggcg tgattttcac caagctacgg tcccggcctt
     1321 tcttcaaggc tttaaccagg ctttgaccgc cgacatcttc gggcaaaatc tcgacgcgtc
     1381 ccccaaccag caacggagga tacaagcccg tgaccgtcag gtcgaacgat gtcgaagtgt
     1441 gcacgggaat cgagccgccg gtctgaaccg aatacgtctt gatggcccag gtgaggtaat
     1501 tcaccaagcc gctctgaaca atcatgactc ccttgggcgt gccggtcgat ccggaagtgt
     1561 acatgacata agccaggttc gcgggagtcg atgtgggctg aagattgctc aaactttcct
     1621 tggcactctg tgaccagtca gcaaccagtc aaaatgttac gccagcagcg gaagcgaaga
     1681 gcggcttatg cttgctgtcc gtcagaagaa cttttgccgc agagtctttc accataaagg
     1741 acaggcgatc cgccggatag gcgggatcga gaggcacgta agcgccgcct gctttccaga
     1801 tgcccaatag cgcgaccagg acttccgggc agcgttccaa acacacgccc acgagcacgt
     1861 caggtccgac gccgcgcttc cgtagaaaat ttgctacctg gttggcacgc tggttcaatt
     1921 ccttgtaggt cagttgccga tcttcaaaaa ccaccgccac agagtcagag tgttgggcca
     1981 cctgctgctc gaacaactgg tgcacgcatg tttgcgggaa ttccgcgcgg gtggaattcc
     2041 agtcgtataa aatttggatt cgctcgcgct cggtgagcat gggcagctcg gaaatgcact
     2101 gatgcggatt gagagcgatg gacgtcaaaa ttgttgtgta atgcccacac atacgctgaa
     2161 tggattgagc tgcgaaaata tcggtgctgt actcaatctt gcccgaaaga ccatttgcgg
     2221 tttcagagag gatgagcgcc agatcatatc cggagctcgc ggcattcact aaggatttgt
     2281 cgctgccctt cggcgcctgc gcggcttcgg cggcggtatg gaaagcgaac aggacctgga
     2341 ccactggagc atggctcgaa tcgcgctccg gcgccaattc ggctacgagc tgatcgaacg
     2401 gaacatccgc gtgggcgtgg gcggccatag tgcgctcccg tacttgttgc agcaggccac
     2461 gaaaattcag atgctcagag aattttgagc gcaaaacagc agtgttctgt aaccgattgc
     2521 gcgaattttc ggtctctccc ggcacgcgtc cgagaactgg agtaccgata aggatatcgt
     2581 cctggcccgt atagcggtgg agcatggccg cgaaaccaga cgcaagaatc atgaagaggg
     2641 tcgattgctc ctggcggcct acatcctcta atggtctcag cagatcctga ggcagctcga
     2701 aggtttcggt cgctccgcgc aaactctgca tggcagggcg tggctggtca gtgggcagtt
     2761 ccaggtttgt cggagcccct gccagttctt ccttccagta cgaaatctgc tcgagcaaaa
     2821 ggtcttcctg tatgttcatc gcgatgattc cgtctcttcc ctcatgattt tgttcactcc
     2881 ggcgcatcat tcgctccgcg ctgacatcaa accatttgca ggcaagcggg agttctttgc
     2941 tgtaatcctg gttaccgcgg atctgaacac gttcatcaag ctttggcctt agcgacttcg
     3001 cgcattcagc acgagatgac cgtcagcaaa tggctcgcca ccagtactac tggcaattga
     3061 tctgccagcg tcagattcac tctaatgcct gtaataccat caacttagct agacatggaa
     3121 cactttcaat ggaaatcgat agccaagaat ggtaataaaa atactacttt tatcctgttc
     3181 ggccggaagc gggaaagtaa cccgttccga taaataacct gggctctcgt gctgctactg
     3241 cgggtcgcgc cacgcattta ccagaagcaa aaacagcgtc cccagattca gagtcgaggc
     3301 gctgcgtcga attgcggatt tcgatagtaa cagcgccaag gttcgaaaaa ccgccaaaat
     3361 acacggtaaa acgccccggt ttacaaaatg gggattatcg aattggaacg tgcggccaat
     3421 tttcaagatc acatccgttg aagagtggtt cagaatgcag agctaggttc agatgccttt
     3481 tgtctttggt atctgccgga gcgcactgtc tcgcttgtgt tcagcaccgt tttctccact
     3541 ggcacggcgt gagttttgtg ctgcaataga gttcccatct aagattagca aacaagccga
     3601 agggttttaa tcttaccctt cgcccttgcg atggagcgga ccatatgtgt ggcattctcg
     3661 caattcttaa cctcaacccc gttcgtccga ttaacacgga gttgcggacc caagccttga
     3721 ccatggtcaa gaagatccgg catcggggac ccgactggag cggcactttt agcgacgagc
     3781 aggccatcat ctgccacgag cggctctcga tcgtggacgt cgagcacggc gcgcagcctc
     3841 tgatcgatcg tcaaaaagga accgtcctcg ccgtcaacgg cgaaatttat aatcacaagg
     3901 atttgcgcga ccaactgaag cagccccacg aattccaaac agcttcggat tgcgaagtgc
     3961 ttctatatct ttacgacgaa gagcacccca agaagtttct caatcgcgtg aacggaattt
     4021 ttgccttcgt cctgtacgac cctaagaaaa agactttcct gatcgcgcgc gatccgatcg
     4081 gagtgaatcc gctttatgtg ggatgggacc gccacgaaaa tctctatgtc gcttcggaaa
     4141 tgaaatcact ggccggtgtt tgcgaacgca ttctggaatt tcctccaggg cattactacc
     4201 ttggtaatga agcggacaaa ggatttcaaa agtattacga accgaagtgg gctgaacccg
     4261 gattctgccc gtccgagcct tatgatccgg cgcgattgcg tacagctctg gagtcggccg
     4321 ttcatcgcca actgatgagc gatgttccgt acggcgtgct catttccgga ggcatcgatt
     4381 cctcgatcgt agctgccgtg gccgcgaagt tcagcgcacg acgcgtggat gagaacgatc
     4441 tttctcccgc atggtggccg cggttgcatt ctttcgccat cggactcgaa ggcgcgccgg
     4501 atctattgcc tgcgaaaacc gtggccgatc atatcggctc ggtccatcat gaaattcact
     4561 ttaccgtgca agagggactg gacgccttat cggatgtaat ttatcaactc gaatcttttg
     4621 atattacgag catccgcgcc actacgccca tgtacctgct gatgcgcaag atcagaacga
     4681 tgggcattaa gatggttttg tccggtgaag gcgcggacga aatttttggg ggttaccttt
     4741 atttccacaa ggctccgaac ggcagagagt tgcacgaaga aacggttcgc aaacttcaga
     4801 agctgcatct ctacgattgc gcgcgcgcga ataaagcgag ttccgcgtgg ggcgttgagg
     4861 cacgcgtgcc atttcttgac cgcgaatttc tcgacgtcgc catggtgctc gatccagacg
     4921 tcaagctacc caaaaacgcc aagcataccc ggcccatcga gaaatggccg ctccgcaaag
     4981 cctttgaagg gtacataccg gattcagttt tgtggcggca gaaagagcag ttttcggacg
     5041 gcgtcggata cagctggatc aatgggctga aggcgaccgc ggagcacgaa atcacggacg
     5101 gcatgatgcg cggcgcggcc gagcgcttcc ccgtgaaaac tccgcaaacc aaagaagcct
     5161 atttttaccg ccagattttc gagacccatt ttccatgggc cacagccatc aattgcgtgc
     5221 cctgggagcg cagcgtcgct tgcagcacgg agacggcgct caagtgggat gcagcttttc
     5281 agaataacgt ggatccttcc gggcgcgccg ttgtggatgt gcacgcggac gcttacgaag
     5341 ttcctacgaa agtttagctg cgcatacgct actttcatgg attttgtagc gctggcgtcc
     5401 cgccggcagc ttttgcgacg cacaggcaga gaaaaacccg gtcgaatcaa aaacgattgt
     5461 tcgattctcc gccaaatttc tgatgaattc ttttttcgca cgataacata taattcacca
     5521 tgcccaacta cgaatcgccc tggatgaacg aagaactgcg gatgtttcgc aaggcgctcc
     5581 gcgaatttat tcaggaagaa ttcgtcccca aacaatcgaa atggcgcgag caacacaggc
     5641 ctgatgtgga ggcctggaag caagccggtg cgaccggcct gctgctcgcc gatattcctg
     5701 aagagtatgg aggcgggggc ggtacgttcg cgcataatgc cgtcgtgacg gaagaattgg
     5761 cgcaagcggg agtgaatttc ggcacgggcg tgcagagcat cgtctcgcac tacatacttc
     5821 aatacggaaa cgaagagcaa aagaaaaaat ggctgccggc aatggcgcaa ggcgaactca
     5881 tcggagcgat cgccatgacc gagccatcgg caggctccga cttacaagcc atcaagactg
     5941 ccgcgcgcct cgaaggaaat gagtacgtca tcagcggctc caaaacattc atcaccaatg
     6001 gcgggctcgc caacctcgtc tgcgtagccg caaaaaccaa tcccaaagtg gcgggtttcc
     6061 gcggcatctc catgatcgtg gtcgagacga aaaatcttaa gggataccgc gtcggcaaat
     6121 cgcttgaaaa agtgggcatg cacgagatcg atacctgcga acttttcttt gacgatgtgc
     6181 gcgttccgtc cacgaacttg cttggaaaag gcgagggagc gggattcgcg caaatgatgg
     6241 atcaattgcg ctacgaacgc ctgatgatag gcgtgggcgc aatcgcatcg atcgaacgcg
     6301 ctctcgacct caccatccaa tacgcgaaag aacggaaagc ttttggccag acgctcatgg
     6361 aatttcagaa tgcgcgcttc aaactggcgc agtgcaaaac ggaagcgcag attggccgcg
     6421 tattcattga caattgcgtt atgcgatttc tgaccggcca gctggacccg gtgaccgccg
     6481 ccatggcgaa gtactggacg acagaatgcc agtgccgcat tgtggacgag tgcgttcagt
     6541 tgtttggcgg atacggctat atgtcggaat acccgatcgc acggatgtgg atggacggcc
     6601 gcgtacagcg catttacgct ggaacaaatg aaattctgga agagttgatt ggctggtcgc
     6661 tctaaagttt cacaccgcgc acaccgctac aaaatttagt cgctaacgtt tttcttcccg
     6721 gaatattccg cattaaacga acggtcaatc tcttcgcgca gctgcccgag aatttctttt
     6781 gatagctgcg ccacatcggg atcctcgaca tgcctcggtc ggtcgagcac aacttcaaac
     6841 tgggccttga ttcttcccgg acggaaactc atgagcagca cgcgatctcc gaggcgcacg
     6901 gcttcgcgga cgttatgcgt gacgaaaaca atcgttggcg aagtctcttt ccagatacgt
     6961 tccagttcat cgtgcagcat gtctcgcgtt tgcgcatcga gcgcagcgaa aggttcgtcc
     7021 atcaataaaa ttttaggacg cagagcgagc gaacgcgcaa gggccacgcg ctgcttcatc
     7081 cccccggaaa gctggtgaat gtagtgatcc tcaaaatggg agagatgaac catgcgcaga
     7141 aaaattctcg cgcgttcttt tctttccgct ttcgacacgc cggccatttt caatccgaat
     7201 tccacgtttt gccggaccgt cagccacgga aataatccta attcctggaa aagcaaaatg
     7261 cgatccgttc ctggaccttt gaccggcccc ccgtcgacaa taatctgtcc cgtcgtgggc
     7321 ttgtccaggc cagcgatcaa atgcaggagc gtggattttc cgcagccgga tggccctacg
     7381 atgcagacaa attccccttg ctcaatttcc agatcgatat tttgc
//
LOCUS       DASZMB010000190         3802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1002632, whole genome shotgun sequence.
ACCESSION   DASZMB010000190 DASZMB010000000
VERSION     DASZMB010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002632"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VG272_08665"
     CDS             complement(<1..921)
                     /locus_tag="VG272_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13792.1"
                     /translation="MSQIASQPSAVQSMMYSKEASEKRHRTRLLIAWILSMTFVAAFL
                     VYGFDYYTTSWKNRPFSPKHFILRPSGTIGVDMGMLGVLMFLIIFLYPLRKRWGWLGK
                     QGNSRHWLDFHVILGTAAPLVIALHSSFKFGGIAGMAFWIMTAVTLSGFVGRYLYSQI
                     PRSLNAAELNVKEIQEAEVQLRWNAEEKHDKIGTTLIDLFALPTSEQVARTPMFAALC
                     WMIYLDLKRPFQISLLRLRAAGFGAWLGSFFGLFLTRNPELEFAIQTARRQASLAKRI
                     LFLSRTKQVFNLWHVVHRPFSYSFAILAILH"
     gene            complement(918..1781)
                     /locus_tag="VG272_08670"
     CDS             complement(918..1781)
                     /locus_tag="VG272_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13793.1"
                     /translation="MIRFFPNLLAGLRTVAALRWLAGFAFAASLIGIPAACRAQATPG
                     GIVAADSSSLDALAHARKYVQEYFDKFSDVTCKESVTQFVLNQSGHTIYRENSAFDYQ
                     FQTTSTSGSLKFNEIRETRNPAYRDPARTLLVTTGFASLLLVAHPMYEASYVFEPAGS
                     EMLDGTSYAKLHFSSVAGASSPATLRLRGKNYPLPMSGTLWVDSQSGAIVKLEAIVDN
                     GLSDLGLAGMRSEVRYGLHTFRDPAESIWVAESAVIEVQTPRQHWRNQHRFSDYKRFN
                     VNIQEKMGKLP"
     gene            complement(1778..3640)
                     /locus_tag="VG272_08675"
     CDS             complement(1778..3640)
                     /locus_tag="VG272_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13794.1"
                     /translation="MVNCKPTVWHLRTIGCAVVLAISFCSARAAQAAQSQAAPSAPSA
                     GALTIAPALDLSPAIPTAQEPASAATPPKIPTKNVFHVKYISESAIYLDAGRNEGLDE
                     GMMLHIVHEDPGGGTTDAVRFQGQEPIADVRIFSVADTSAAAAIIKSREDLVKGDVAY
                     LDIDSAHLRENIADAAEAVNYPVVVTFSYGDPLDEEIRADTSPVKTASLQNQIRGRIG
                     FDYGNLSEPGGISSRQLGLLIDADMSHIGGTHWNFTGYWRGNLETQNTGNTTGISPVT
                     LNDLVNRTYHLGLYYQNPDSIITMGVGRLFLPYAPSLSTIDGGYFGYRFTSHLTAGVF
                     GGSTPDPTSWSYAPDQNIAGTFLNYEKGDFDHLRFSDTVGLAATSLAWHIAREFVFAE
                     NNISVGHLFSIYNSLQVDKGRTALSGSTYNAGLTQSFSSARYQPVRFLTLSLNHNYLR
                     SLPTFDPALISLGLLDQYLFQGFSGGVHIDLPFKIGVSTDIGRSKNSTDTGTSWNQAY
                     GLTFGEIPRTGMQMDLRYTKFNSSFGQGNYQFISLSRTLAERFHVQLQGGMQHLTSSF
                     STNSDSKFVSSSVDWSIGPRYFFEGLFSWNMGTTMNYQQTNFTFGYRFGGSLRK"
BASE COUNT          889 a         1003 c         1067 g          843 t
ORIGIN      
        1 gtgcaggatg gcgagaatag cgaacgaata actaaacggg cgatggacga cgtgccaaag
       61 attgaaaacc tgcttggtgc gcgacaagaa tagaatacgc ttggcgagcg aagcctgcct
      121 gcgcgcggtc tggatcgcaa attcgagttc ggggttgcgc gtcaaaaaca gtccgaagaa
      181 ggaaccgagc cacgcgccga aaccggcggc acgtaaacgg agcagagaaa tttgaaatgg
      241 acgcttgagg tcgagataaa tcatccagca aagcgccgca aacatgggag tgcgcgcaac
      301 ttgttcggaa gtcggcagcg caaatagatc gatgagagtt gttccgatct tgtcgtgctt
      361 ctcttcggca ttccatcgca gttgcacttc ggcttcctga atttccttta cgttcagttc
      421 cgccgcgttc agcgatctcg ggatttgcga gtacaagtat cggccaacaa aaccgctcag
      481 cgttacggcg gtcatgatcc agaacgccat gcccgcaatg ccgccgaatt tgaacgacga
      541 gtgcaaagcg attacgagcg gcgcggcagt gccgaggatg acgtggaaat caagccagtg
      601 gcgcgaattt ccctgcttgc cgagccaacc ccagcgcttg cgaagcggat acagaaaaat
      661 aatcagaaac atgagcacgc cgagcattcc catatccacg ccaatcgtgc cgctcgggcg
      721 aagaatgaaa tgcttaggag aaaagggacg attcttccaa ctcgtggtgt agtaatcgaa
      781 cccgtagacc aggaaggctg cgacgaacgt catggacaga atccatgcga tcagcaggcg
      841 tgtgcggtgt cttttttccg acgcttcctt gctatacatc atgctttgaa cggctgatgg
      901 ttgactcgcg atttggctca tggcaatttc cccatttttt cttgaatatt gacgttgaat
      961 ctcttgtagt ccgaaaagcg atgctggttg cgccagtgct ggcgcggagt ctgcacttca
     1021 atcacggcgg attcagcgac ccagatggat tcggcgggat cgcgaaaagt atgcagcccg
     1081 taacgcactt cgctgcgcat tcccgcaagt cccaggtcgc tcaatccgtt gtcgacgatc
     1141 gcttcgagct tcacgatggc gccgctttgc gaatccaccc aaagcgtgcc ggacatggga
     1201 agcggataat tctttccgcg caaacgaaga gtcgccgggg atgaagcacc tgcaactgaa
     1261 gagaaatgaa gtttcgcgta cgatgtaccg tcaagcattt ccgagcctgc cggctcaaaa
     1321 acgtagctcg cttcgtacat gggatgcgcg acgagaagca ggctcgcaaa tcccgttgta
     1381 accagtagcg ttcgcgccgg atctcgatag gcgggattgc gcgtttcgcg aatttcgttg
     1441 aattttaaac tgccgctcgt gctcgtggtc tgaaactggt aatcgaaggc cgagttttcc
     1501 cgataaattg tgtggccgct ttgattcaga acaaattgcg tgacggattc cttgcaggtg
     1561 acgtcggaaa atttgtcgaa atattcctgc acatattttc tcgcgtgcgc cagggcatcg
     1621 agcgaggacg aatcggcggc cacaattcct ccaggagtcg cctgtgcgcg gcacgctgct
     1681 gggattccga tgagcgatgc cgcgaacgcg aaaccagcga gccatctcaa cgcggcaacc
     1741 gttcgcaagc ccgcaagcaa atttgggaag aagcgaatca tttgcgcaac gatcctccga
     1801 agcgatagcc gaacgtaaag ttcgtctgct ggtaattcat ggtcgtaccc atgttccagc
     1861 tgaaaagccc ttcaaagaag taccgggggc cgatgctcca atccacggaa ctgctcacga
     1921 atttcgagtc ggaattcgtg ctgaaggacg acgtcagatg ctgcattccg ccctgcaact
     1981 gcacgtgaaa gcgctcggcc agtgttcgcg agagcgaaat aaattgataa tttccctgcc
     2041 caaacgaact gttgaatttc gtataccgca ggtccatctg catgccggtc cggggaattt
     2101 ccccaaacgt caggccgtac gcttggttcc aggaggtccc cgtatccgtg ctgtttttgc
     2161 tgcggccgat gtccgtcgaa accccaattt taaaaggcaa gtcgatatgc acgccgccgc
     2221 tgaatccctg gaacaaatat tggtccagta atcccaaact gattaacgcg gggtcaaacg
     2281 tgggcaggct gcgaagataa ttgtggttca aactcagtgt tagaaatcgg acgggttgat
     2341 accgggcgct cgaaaaactc tgtgtgaggc ctgcgttgta ggtgctgccg ctcagggccg
     2401 tgcgaccctt gtcgacttgc agcgaattgt aaattgaaaa gaggtgtccc acggaaatat
     2461 tattttcagc aaaaacaaat tcccgggcga tgtgccaggc gaggctcgtt gcagcaaggc
     2521 cgacagtgtc gctgaaccgc aagtggtcga agtctccctt ttcgtagttc aggaatgttc
     2581 ccgcgatatt ctggtccggc gcgtagctcc acgacgtcgg atccggcgtc gagccgccaa
     2641 acacgcctgc ggtaaggtgg ctggtaaatc ggtagccgaa ataaccgccg tcaatcgtgc
     2701 tcaggctggg cgcgtacggc aggaagagtc gtcccacgcc catcgtaatg attgaatcgg
     2761 gattctggta atagaggccg agatgatacg tgcgattcac caaatcgttc agcgtgacag
     2821 gagaaatgcc ggtggtattg cctgtattct gcgtttccaa atttcctcgc cagtagcctg
     2881 taaaattcca gtgcgtcccg ccgatgtggc tcatgtctgc atcgatcagc aggcccagct
     2941 gccgcgagct gattccgcca ggctcggaaa gatttccata atcgaatccg atgcggccgc
     3001 gaatctgatt ctgcagggaa gcagttttta caggcgaagt atcggcgcgg atttcttcat
     3061 ccaatggatc gccatacgaa aaagtcacga ccaccggata atttacggct tccgccgcat
     3121 cggcgatgtt ttctcgcaga tgcgcgctat cgatatccag gtaggcaacg tcccctttaa
     3181 ccaggtcttc gcgcgattta ataattgcag cggcggcgga agtatcggcc acggaaaaaa
     3241 tgcgcacgtc cgcgatgggt tcctgtccct gaaatctcac agcgtcggtt gttccgccgc
     3301 ccggatcttc atggacgatg tgcagcatca ttccttcgtc gagaccttca ttgcgtcccg
     3361 catccagata gatggcgctt tcggaaatat atttcacatg aaaaacattt ttcgtgggga
     3421 tcttaggcgg cgtagctgct gacgcgggct cctgcgccgt gggtatggcc ggcgaaaggt
     3481 ccaaagctgg cgcaatggtt agcgcccctg cagagggagc ggaaggagca gcctggctct
     3541 gcgccgcttg cgcggcgcgc gcgctgcaaa acgaaatcgc aagtacaacc gcgcaaccta
     3601 tcgttcgcag atgccatacg gttggtttgc aattaaccat tggggtggca cccggatttc
     3661 gtacacgtca tgggtccata gtttccagcg ttgtgctggc ctgaattctt tacccagctc
     3721 gattcgggtg tatggcaccc atttgtgcaa ttgaacgtgg cgaaatttgt agctgcgccc
     3781 ggagtctggt gacaagtctg gc
//
LOCUS       DASZMB010000191        18788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3222570, whole genome shotgun sequence.
ACCESSION   DASZMB010000191 DASZMB010000000
VERSION     DASZMB010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 18788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18788
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3222570"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..533)
                     /locus_tag="VG272_08680"
     CDS             complement(228..533)
                     /locus_tag="VG272_08680"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HWF13795.1"
                     /translation="MAQTGSDQVQVQIKADEKELVGQFSNLVMFHHTAEEFTVHFIYV
                     FPNVPQGKLVSSVIVSPGHAKRILHALEENIRRYEKQFGPIREVPDPTPVPNVGFVQ"
     gene            773..2176
                     /locus_tag="VG272_08685"
     CDS             773..2176
                     /locus_tag="VG272_08685"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HWF13796.1"
                     /translation="MSFVPSSPASLDPISGTGGANAVAAATPACLPSDAGSPSLPESY
                     RSIPILASASFFRKMIAFAGPGYLVAVGYMDPGNWATDLGGGSRYGYTLLSAVLISSL
                     MAIFLQALSAKLGIATGRDLAQACREHYSRRTTAVLWVLCEIAIAACDLAEVLGSAVA
                     LKLLFGLPLLAGVLLTGLDVLLVLALQGRGFRVLETLVITLILTIGACFAYEIFFARP
                     LWIEAAQGFVPTFQILKDRQMLYLAIGILGATVMPHNLYLHSSIVQTRAFSLTAEGKR
                     EAVRFATLDSTIALGLSLFINAAILVLGAAAFHVRGMNDVADISQAYQLLTPVLGVSL
                     ASVLFAAALLCSGQNSTLTGTLAGQICMEGFLHWKVSPWLRRLITRSMAIIPAALVIG
                     LMGENHTTSLLILSQVILSFQLPFAVVPLIQFTGDRSKMGEFTNSPITSIFGWFLAAA
                     IILLNAALLWLTFGGKL"
     gene            2520..3413
                     /locus_tag="VG272_08690"
     CDS             2520..3413
                     /locus_tag="VG272_08690"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HWF13797.1"
                     /translation="MSARSQETQYPKPEDLPNPYKLVEGWPTLPANMNGGHWGEVIRV
                     HLEHNGNVWVFHRCFSVVPPGHATCVGRGEANPPILEFDPAGKLIRSFGDGLFAYPHG
                     FTVAEDGNLWATDVNDEQQILGLPAKNKDGVVMGQEVLKISPMGKVLMTIGKEGVSGD
                     GNDTFDRPTGVAVAPNGDIFVSDGHYPNAHHNARIMKFSKDGKFIKSFGHLGSGDGEI
                     DEPHDIAIGGTQNRVYVANRRNRRIEVFDQDGNFITSWKQFGQPSSVFVGKDDTIYVG
                     ASFWDAKDQIEVRAKARVRKC"
     gene            3361..3633
                     /locus_tag="VG272_08695"
     CDS             3361..3633
                     /locus_tag="VG272_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13798.1"
                     /translation="MRKIKSKCGPKHASENVKDYIELTKREKRGVIIGSVKDGSLKAF
                     IPDPADLTKLTDVGSSASGIAADDTGNTIYVADVAVHNLRKYVKVQ"
     gene            3817..4275
                     /locus_tag="VG272_08700"
     CDS             3817..4275
                     /locus_tag="VG272_08700"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWF13799.1"
                     /translation="MRGFNKGTSRWLVRATGACLVLGMFVSVGGRVLADDNPPAPWVA
                     PDDAKKVKNPVPSTPEALTAAQDLYTDNCLLCHGEKGVGDGPGAKAIKVKPANFTDAK
                     MMAAETDGSLFWKMTNGRGPMPSWKDTLSDKERWELVDYIRKLGKDGAKK"
     gene            complement(4374..4835)
                     /locus_tag="VG272_08705"
     CDS             complement(4374..4835)
                     /locus_tag="VG272_08705"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HWF13800.1"
                     /translation="MPIAVGQTAPEFTLKDQSQKEIKLSDYAGKRNVVLIFYPLDFSP
                     VCTNEHACFVNDLKQYDSLDAQVLGLSVDSAWAHKAFAEKMGITYPLLADFHPKGAVA
                     DKYGMYLADKGITGRAIAIINKAGKIAWFKNYDIPVMPDMKEVGQALAAVK"
     gene            5164..5784
                     /locus_tag="VG272_08710"
     CDS             5164..5784
                     /locus_tag="VG272_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13801.1"
                     /translation="MTRVSLAQAYPGISIRPSSRRLWARFMRWPAECIHLDKGHHWMA
                     TYIPDTLYLRGKARTRREPRRPEISVCFDCLSGLLEPELAATSARLVAFEPDGESFTQ
                     YFFLSREDFAAAGLRSEVGAAIEARLSKLEGPCSDCGDSATWLWISRREVSNLDQAER
                     IETSPGALFCAKHGAAKLCGALGQIDEANLFYVNAPYGDSGAYVWI"
     gene            5789..6031
                     /locus_tag="VG272_08715"
     CDS             5789..6031
                     /locus_tag="VG272_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13802.1"
                     /translation="MTLRELVEKYLAIAGGFTFLAPLADFGMERQEVERVFGAFDEDY
                     HISRYIHFTQVSGPAYKINGFEQTHVSMDEEIQTIL"
     gene            complement(6281..6487)
                     /locus_tag="VG272_08720"
     CDS             complement(6281..6487)
                     /locus_tag="VG272_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13803.1"
                     /translation="MRHLNPALRIFAALAGASLSPGAAWACPACMVGDPKTAGTYLGM
                     TLVMSSLPILLVCGLGYWLWRRYQ"
     gene            complement(6495..7076)
                     /locus_tag="VG272_08725"
     CDS             complement(6495..7076)
                     /locus_tag="VG272_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HWF13804.1"
                     /translation="MESRMAGSAPNAGNRLPNSVLAMIIFVVMEIMFFAGLISAHTIA
                     RATVLGGVWPPVGQPRLPVERTAINTAILLLSGALLYVANRRSRDSYAKALPYLAGAI
                     ATGIAFVLFQGVEWVALLREGLTMTSGAHGAFFYLIVGAHALHAIVAISVLTAIYFPM
                     RKGSLEPSTFTAVQIFWYFVVLVWPIIYLRVYL"
     gene            complement(7090..7443)
                     /locus_tag="VG272_08730"
     CDS             complement(7090..7443)
                     /locus_tag="VG272_08730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HWF13805.1"
                     /translation="MTTESHVHHPNYVKIWAILVVLLAVSVLGSMSHVREVVLIAAFG
                     IALVKAYLVAKNFMHVNVEKRWVPYLLVVCLLLICILFAGVAPDVMKQSGEHWTKNPI
                     DVQARSAPNAPVKHE"
     gene            complement(7500..8081)
                     /locus_tag="VG272_08735"
     CDS             complement(7500..8081)
                     /locus_tag="VG272_08735"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HWF13806.1"
                     /translation="MSASAKPIANTRSVTGIPTARLITWWVIASEIVIFGGLLAAYIM
                     HRLGHPEWAEAASHTNAWAGASNTLVLLTSSLCAVSAHKAADALDGLRAAKLLRLTVL
                     GGLLFLTIKGFEWTKDIHEGFVLSSGGFWPFYYVLTGLHGLHVLAGMIIMLIVASDAA
                     KNQELQRVECIGLYWHFVDIVWIFLFPLLYIAR"
     gene            complement(8102..9796)
                     /locus_tag="VG272_08740"
     CDS             complement(8102..9796)
                     /locus_tag="VG272_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HWF13807.1"
                     /translation="MSTPVAMNTHLERVEPGIFKKYFWSTDHKVIAMQYLFTGMAMAM
                     IGAFFAYVFRMQLGFPNQNVPGYGFVTPGRYNALVTMHGSIMIFWVAMPVLIAAFGNF
                     LIPLMVGCDDMAFPRINRLSYQIFLISAIVLLASFLVKGGAFGGAWTAYPPLSANGAY
                     NLTPLGSVLWLLAVVLEFVAFLLGGINFTVTTMNSRAPGMKMYDIPLVIWMIVLASII
                     FMASAGPLIAGAVMLLFDQTMSTGFYDPTRGGDPLLWQHLFWFFGHPEVYVVLLPAMG
                     IVAEIIAVFARKKIFGYKTVLYTTWAVGFLSFVVWAHHQFVAGIDPRMANIFSITTMM
                     ISVPITEIVFLYIATLYGGSITLDTPMLWALGFVGEFMIGGVTGIFLGSSGTDIYLHD
                     TYFVIAHFHYTFVPIAIISVFAGITFWYPKMFGTMLNETLGKIHFWGTVIPFNVIFIP
                     LFITGTAGDQRRVYSYAQFAEQSRPALQHLRTTSTIALLVMLSFQFVFLFNFIWSLRH
                     GKKAGANPWRSATLEWTVPSPPPHGNFAELPTVYRGPYEYGLPGRADDFTPQSVPE"
     gene            complement(9793..10530)
                     /locus_tag="VG272_08745"
     CDS             complement(9793..10530)
                     /locus_tag="VG272_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13808.1"
                     /translation="MYERYLSAASTYAHSIDHLFILIGVIVGLWLIAAETMMFVLIFK
                     FRAKDGRPTQYITGDEKHLKRWITIPHLLVLVCDVVIIVGAISVWYNIKQNMPAADET
                     IRVIGQQWAWSFQQAGPDGVMDTADDIRSVGELHVKVNTTYHFDLMSRDVVHSFAVPA
                     FRLKQDALPGRTITGWFRPNKVGTYDIQCTQMCGLGHGLMAGRIIVETPQDHAAWLMR
                     NTNPAVAGLPYTSPTSDGAMTTAEVRR"
     gene            complement(10676..11344)
                     /locus_tag="VG272_08750"
     CDS             complement(10676..11344)
                     /locus_tag="VG272_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWF13809.1"
                     /translation="MSKTQTLVPNRFSAWVFFLLVAGCALHLSGCGWGSTDAATRGKE
                     VFQTCVPCHSTDGSGNATVGAPNIAGMKEWYVEEELDKFRGGVRGMQFTDMEGMRMRP
                     MALSLASEDDVKSVAHYVESMPPVRHAATLPGDPKAGEAIFATCAACHGDNGAGNEDL
                     KAPPLAGVDDWYLATELRKFRSGARGTSPKDSGGRLMRPMARGLANEDAIRNVVAYVG
                     TLKR"
     gene            complement(11518..12618)
                     /locus_tag="VG272_08755"
     CDS             complement(11518..12618)
                     /locus_tag="VG272_08755"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HWF13810.1"
                     /translation="MAEHMLILGLESPDGKVTYMAAAFPSACGKTNLCMMVSALEKEG
                     YRVWTVGDDISWMQVGADGYLHAINPEAGFFGVAPGTNDKTNPNVMSALRKNTIFTNV
                     AMTPQHEPWWEGMSDNIPSDMLDWQGRQWNPSNGPAAQANSRYTVPAGQAPSISPRWE
                     DPAGVPISAFIFGGRRARLAPLVYEAMNWQHGVYVASTMASETTAAITGAVGVTRRDP
                     MAMLPFCGYNMADYFGHWLEMGRNIPHPPKIFHVNWFRKGADGKFLWPGYGENARVLK
                     WMLERIEGKGAATETPIGAVPTTNAITLDGINVSKDTMNELLRVDPADWVKEHADTGN
                     FFEIFGDRLPEEISEEHTRLGERLQRTAVAQK"
     gene            complement(12567..13277)
                     /locus_tag="VG272_08760"
     CDS             complement(12567..13277)
                     /locus_tag="VG272_08760"
                     /inference="COORDINATES: protein motif:HMM:NF036305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13811.1"
                     /translation="MSTPLEQWVESVAKMTRPSQIQWCDGSEEEDARIKDQLVREGQT
                     FPLNEKTYPGSILHRSDPSDVARTEKVTYICSKDRDEAGPTNNWMAPAEAKKHMGSLF
                     EGSMKGRTMYVVPYIMGPAASPQSRIGVELTDSAYVVASMRIMSRMGKVALDRLGNSS
                     DFVPGLHSLGDLSPDRRLIAHFPEEKLIWSIGSGYGGNALLGKKCFACALPVPWRESR
                     VGWRNICSSSDSNLLMEK"
     gene            complement(13780..14751)
                     /locus_tag="VG272_08765"
     CDS             complement(13780..14751)
                     /locus_tag="VG272_08765"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HWF13812.1"
                     /translation="MAKRPKKKVINWLQSAVGRVREFAYTEPPPPQVERPRIGVALGG
                     GFARGIAHLGVLRVLEEEKIPVDLLTGTSAGALMAISFASGHTIPEIERQAQATRFKD
                     FGNWKLSWMGLASNQKLEHYPRKYLGVSTFEDLRIPLAIAATDLATGEAVYFSHGPLG
                     PALRASCAYPGMFIPVEIDGRTLVDGFLAAPVPVEAARSIGADLVIAVFLEAANNRKP
                     SSIVDVIGLSFAILQRHADLEWRRTADVTIEPVVKDFLWDDFERTSELLAAGEAAARA
                     ALPKIRAVIAEKTAQKILPLESKEVETIRRVAKNRPEDRLSGTSREK"
     gene            14953..17394
                     /locus_tag="VG272_08770"
     CDS             14953..17394
                     /locus_tag="VG272_08770"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HWF13813.1"
                     /translation="MSKKMIEKVVVLGAGTMGARIAAHIANAGVWCALLDIAPTELSA
                     DEQKRGLTLASMDVRNRIVRTGLAAAVKSRPAAFFKRGVESRIITGNFEDDLKLCAEA
                     DWIIEVVAENLEIKRALLSRVAAVRKPGTIVTTNTSGLPVRSIAEGMPEEWQQHWAGT
                     HFFNPPRYLKLVEIIPGTATLPEVIKMLAGFCDHKLGKGVVVAKDTPNFIANRIGTFS
                     MLNAIRLMGELGMTFEEVDACTGPALGWPKSATFRLADIVGIDVLLHVIRNIHENIPN
                     DESRELYKVPALIEEMAKRGWIGDKTGSGFYKRVKKAGGESEILTLDPVKMEYRPQQK
                     ARFASIEAGKGIENTRERLRMLCGPALEGKEGDKAQKFIWGVLSGMCLYAARRVPEIS
                     DSIVDVDHAMRWGFGWELGPFEIWDGIGVEPMANQLEKEGNALLPLVTDLLASGKKSF
                     YQSAQGETSYFDIAGKTYKPAPQPEGIIILKSLKERAREIEKNSGTSLIDLGDGVICC
                     EFHSKMNAIGGDLLQMIHKGLARLRTDFDAMVIANQGENFSAGANLMLVLMTAQEQEW
                     DDLHVMVRQFQNTTLALKHSPKPVVVAPQGLALGGGCEVVLHGTRVHAAAEAYVGLVE
                     VGVGLIPAGGGSKEMIIRANERAEGEEDLDLFHALKPVFENIAMAKVSTSGEEARDLG
                     YLRRADLVAMNLDRLVADAKETALGLVRSEWRQTAPAPNEPSIRVLGQEFSAAAKLAV
                     HMMLRGEYISEYDAFVARKLGHILAGGALTSAQFVSEQYLLDLEREAFVSLCGERKTQ
                     ERIAHTLKTGKPLRN"
     gene            complement(18034..18633)
                     /locus_tag="VG272_08775"
     CDS             complement(18034..18633)
                     /locus_tag="VG272_08775"
                     /inference="COORDINATES: protein motif:HMM:NF014752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ERCC4 domain-containing protein"
                     /protein_id="HWF13814.1"
                     /translation="MRRRRTLITSPLEWQMTALPVAHPIVPVIAERGGTQLRTPKPAI
                     VVDTREQNPFSFARFSGWFSGIEKRPLEIGDYSVAGLEDICVVERKDLSDLVCSFHME
                     RSVFVARLRRMSAFPDRLLVITAALSQVKSRYEHSPANPNQITQSLIATLAGLRVPFL
                     CVETHELGEEIVASYLYQVHLYHWLEANGHDRRLADDDL"
BASE COUNT         4357 a         4849 c         5320 g         4262 t
ORIGIN      
        1 gcaaaatagc ccgcctgatg caggctttac cgctctaatt taacatgggt agcttcggac
       61 tcatgcgaac ttcaactgct aatgtttcga taagtgaact aagtatgtaa gccacttata
      121 actaaggtgt tatcgttcgt atggcgaaac acaaacccat attggattgg ggtaaattcc
      181 gcaccggcct tgcgtgattt cgatttgcaa aaaggaagcg aaatttccta ctgtacaaaa
      241 ccgacattgg ggacgggggt agggtcggga acttcgcgga taggcccgaa ttgtttttcg
      301 taccgcctga tattttcctc gagcgcgtgc agtatgcgct tggcgtgtcc cgggctgacg
      361 atgacgctcg aaaccaattt gccttgcggc acgtttggaa aaacataaat gaaatgcacg
      421 gtgaactctt ccgccgtatg gtgaaacatc accaggtttg aaaattgtcc gaccagttct
      481 ttctcgtcgg ccttgatttg aacctgtacc tgatcgctac ccgtttgcgc catgggttcc
      541 tccgttcaca gagatcattc cccgcgaatc gttccaattc cgacactatc acactccacg
      601 ggagcactgc acaaccaaca cgctctaaaa ctccgcataa ctaaaaaaag aagcgaacta
      661 ccccgcataa aaaaacacag ttaatgaatt cctgggcaca ccagggcagc aattacgttg
      721 acgggtgttc ttcttaatgc tagtttatag aagatattta tctaggttaa ctatgtcttt
      781 tgttccttca tctcctgctt ctttggaccc gatatccgga acgggcggtg caaacgcagt
      841 agcggccgcc acgccggcat gccttccttc cgatgcaggt tctccatcac ttccggagtc
      901 ttatcgcagc attccgattc tggccagcgc ttcctttttt cgaaaaatga ttgcctttgc
      961 cggccctggt tatctggtcg ctgtcggtta catggatccg ggaaactggg cgacggattt
     1021 gggcggcggt tcgcgttacg gctacacgct tctgagcgct gttttgatat ccagtttgat
     1081 ggccattttt cttcaggcgc tatccgccaa actcggaatt gccactggcc gcgatctggc
     1141 gcaggcatgc cgtgaacact attcgcggcg aacgacagcg gttctgtggg tactttgcga
     1201 aattgctatt gccgcctgcg atctcgcgga agtccttggt tcggctgtgg cgctcaagct
     1261 tctgtttgga ttgccgctgc ttgccggagt gttgctgacg ggactcgacg tgctgctcgt
     1321 tctggccttg caggggcgcg ggttccgagt tttagaaacg ctggtcatta cgctgatcct
     1381 gacgattggt gcctgcttcg cgtatgaaat atttttcgcg cgtcctctgt ggattgaagc
     1441 ggcgcaagga ttcgttccca cattccagat cctgaaagac cggcagatgc tctacctcgc
     1501 aatcggaatt ttgggcgcaa ccgtgatgcc gcacaatttg tatctgcatt cgagcatcgt
     1561 gcaaacgcgg gcctttagtt taacggcgga aggtaagcgc gaagcggttc gcttcgccac
     1621 gctggattcc acgatagcgc ttggactttc tctctttatc aacgcggcaa ttttagttct
     1681 aggggcggcg gcatttcatg tgcgcggcat gaacgacgtg gcggacatca gccaggcata
     1741 tcaattgctg acgcctgtgc tgggcgtaag ccttgcgagc gtgctgttcg cggcggctct
     1801 gctgtgttcc gggcaaaatt ccacgctcac cggaaccctg gccggacaaa tttgcatgga
     1861 ggggttttta cactggaaag tcagcccgtg gttgcggcgg ttaatcacac gatcgatggc
     1921 cattattcca gcggcgctcg tgatcgggct gatgggtgag aaccacacga cttccttgct
     1981 tattttgagc caggtcatct taagttttca gctgcctttc gccgtggtgc cactgattca
     2041 atttacgggc gaccgctcga aaatgggtga atttacgaat agtccgatca cgtccatttt
     2101 tggctggttt ctggcagcgg ccataattct gctgaacgcg gcattgttat ggctgacgtt
     2161 tggcgggaaa ctgtagacgc acgctggtaa atatccagca cttccttcaa atatatccgc
     2221 agtgcctcaa tttacattgc aagtcgccca cttcgtccca aagaacacgg gacgaagtgg
     2281 cctctacgaa accggaatgc atttgctttc gtagaggccg tccgctgttt ggacgggcga
     2341 ttttttggat tgtagatcat cacaactgtg gcgctcgcgt gcctcagcgg agtaaaatgt
     2401 cgacctattt ataaaaacga agctgtaatt gtgcgttcaa cgggagggtc agaatgttgt
     2461 ccggccacca aatcaggaat gctttcttag cctttttcgc cgtgattgtg atgtctggta
     2521 tgtcggcacg cagccaggaa acccaatacc ccaaaccaga agacctgccc aatccgtata
     2581 agctcgtgga aggctggcca acgcttcccg cgaacatgaa tggcggacat tggggcgaag
     2641 taattcgtgt gcacctcgag cacaatggca atgtctgggt ttttcatcgc tgcttcagtg
     2701 tggtccctcc gggtcacgca acgtgcgttg gtcgcgggga agcgaatccg ccgatcctgg
     2761 aattcgaccc tgccgggaag ctgatcagga gctttggcga cggccttttt gcctatcctc
     2821 atgggtttac ggttgccgag gatggaaatt tgtgggcgac cgacgtgaat gacgagcaac
     2881 agattctcgg tttgccggca aagaacaagg acggcgtggt catggggcaa gaagtgctga
     2941 agattagccc catgggtaaa gtcttgatga cgatcggcaa agagggagtg agcggagacg
     3001 gtaacgatac gttcgatcgt cctacaggcg tggcggtcgc tccaaatggc gacatttttg
     3061 tatcggatgg ccattatccg aacgctcacc acaatgcgcg cattatgaag ttttcgaagg
     3121 atggcaaatt catcaaatcc tttgggcacc tcggatcagg cgatggagaa atcgatgagc
     3181 cgcatgatat cgccattggc ggcactcaaa accgcgtgta tgttgccaac cgcagaaaca
     3241 ggcgcatcga agtgttcgat caggacggta atttcatcac atcgtggaag caatttgggc
     3301 agccgagttc cgtgtttgta ggcaaagatg acacgattta tgtcggggct tccttctggg
     3361 atgcgaaaga tcaaatcgaa gtgcgggcca aagcacgcgt ccgaaaatgt taaggactac
     3421 atcgagctaa ccaagcgtga gaagaggggc gtcatcatcg gcagcgtcaa ggatggctcc
     3481 ctgaaggcct ttattcctga tcctgccgac ctgacaaagc tgactgacgt agggtcgtca
     3541 gcgtcaggca ttgccgcgga tgacacaggc aacacgatct acgtagcgga cgtggccgtg
     3601 cacaatttgc gaaaatacgt aaaggtacaa taaatgggtt gatcgcaact ttaagtaaac
     3661 agggtcttta tgcgaggcaa gtccactgag cagtgccagg atgggtttgt gccggaccgc
     3721 gacctagagg cagactgttt aacaggtgaa ataagctctc ggttgtggca gaatttttaa
     3781 ggtcttgcaa atctcggttc aaatggagac gtcggaatgc gtggattcaa caaaggaaca
     3841 tctaggtggc tggtgcgtgc cacaggcgct tgcctcgtgc tgggaatgtt cgtatccgtc
     3901 ggtggacgcg tccttgcgga cgataacccg cctgcaccgt gggtggcgcc ggacgatgcg
     3961 aagaaagtga aaaatccagt gccttccact ccggaagcat tgactgccgc acaggatctg
     4021 tacactgaca attgcctgct ttgccatggc gaaaagggcg tgggagacgg ccctggcgct
     4081 aaagccatca aggtgaagcc ggcgaatttc actgacgcga agatgatggc tgccgaaacg
     4141 gatggatcac tgttttggaa aatgacaaac ggccgcggcc ccatgccttc ctggaaagat
     4201 actttgtcgg acaaggagcg ctgggaactg gtcgattaca tccgcaagct gggcaaagac
     4261 ggcgctaaga agtaacggcg gaaagtaata aatggattag aagtagaaag aacaagggcg
     4321 aggtatttgc gattcgctaa cgcatcgcaa ccgcgccctg attttttccc ctcttacttg
     4381 actgctgcca gtgcttgtcc cacttctttc atgtccggca tgaccggaat atcataattc
     4441 ttgaaccacg cgattttccc cgctttgttg ataatggcga tagctcgccc cgtgattccc
     4501 ttgtcagcga gatacatgcc gtacttatcg gcaaccgcgc ctttcggatg gaaatcggcc
     4561 agtaggggat aagtgatgcc cattttttcg gcgaaggcct tgtgcgccca cgcactatcc
     4621 acgctcagac ccaggacctg cgcgtcgagc gaatcgtact gcttcagatc gttgacaaag
     4681 cacgcgtgtt cattagtgca aacggggctg aaatcgagcg gataaaaaat caggacaacg
     4741 ttgcgcttgc cggcataatc ggaaagcttt atttcctttt gcgattgatc tttcagggta
     4801 aattccggag cagtttgtcc tacggcaatg ggcatgaagc ctcctaaaga gtatttaaat
     4861 gatccgcaat tgtgattttg tgatgcgcct gctgtaaccc gcctctgaag aggcggggct
     4921 tttccgtgcc tatgagacaa ccgcagctac gaccctcgtc ctgcgcgcca ggcctgtgcc
     4981 ttgcatcttg cccaccttta atctaaagcc ccgcctctga tccttcgttt tcactcagga
     5041 caagagaggc gggctacagc agtttctttt acccagcggt tgcggggaaa acgctgcggg
     5101 caacgattta tcatagcgga tttattctag cgctgtcctg gattctgata ccatcggcga
     5161 gagatgacgc gagtcagttt ggcacaggct tatccgggaa tttccattcg tccaagctcg
     5221 cgcaggctgt gggcgcggtt catgcggtgg ccggcggagt gcatccattt ggataaagga
     5281 caccattgga tggcgacgta cattccggac acgctgtatc tgcgcggcaa agcgagaaca
     5341 cgcagggaac cccgacgacc ggaaatttcc gtttgcttcg attgcctgag cgggctgctc
     5401 gaaccggaat tagcggccac gtccgcccgc ttggttgcct tcgagccgga cggcgaaagt
     5461 tttacgcaat atttttttct gagtcgcgaa gattttgccg ccgcgggatt gcgttctgaa
     5521 gtaggcgccg cgattgaggc gcgcctatct aaactggaag gtccgtgcag cgattgcggc
     5581 gattccgcaa cctggttatg gatttcgcgg cgcgaggttt ccaacctcga tcaggcggaa
     5641 cggatcgaga cttcgccggg agcgcttttt tgcgcgaagc atggggccgc gaaattgtgc
     5701 ggagctttag gacagattga cgaagcaaat ttattttacg tcaatgcgcc gtacggagat
     5761 tcgggcgcat acgtttggat ttaagcccat gacgctgcgc gaacttgtcg aaaaatatct
     5821 ggctattgcg ggtggcttta cgtttctggc gccgcttgcg gattttggaa tggagcggca
     5881 agaagtggag cgtgtttttg gggcgtttga cgaggattac catatcagcc gctacattca
     5941 cttcactcag gtttccgggc ccgcctacaa gatcaatgga tttgagcaga cgcatgtttc
     6001 catggatgaa gaaattcaga cgattttgta ggagttgtgc ggatagtggg cggaaactgg
     6061 catcgtattt tcttagtttc acaattcaaa tcaaaaaaca cattgctgta gcccgcctct
     6121 tcagaggcgg gggtttagat gttgcgtaat ttaaatcccc gcctctgaag aggcgggcta
     6181 cagcagccga tcgatttcac aatggaaaaa agaaggagag attccttgct gcgctcggaa
     6241 tgacaggaga atacttttct cgcaggttga agaggtattt ctactggtag cggcgccaga
     6301 gccagtagcc taggccgcag acgagcagga tgggcaggga gctcatgacc aaggtcattc
     6361 cgaggtaggt gccagcggtt ttgggatcgc cgaccatgca agcggggcac gcccatgctg
     6421 cgccgggtga cagcgatgcc ccggcaaggg ctgcaaagat gcgcagtgct ggattcagat
     6481 gacgcatagg tcttttacaa atatacacgc aagtagatga tgggccaaac caggactacg
     6541 aagtaccaga aaatttggac agctgtgaat gtcgagggct cgagcgatcc tttgcgcatc
     6601 gggaaataaa ttgcggtgag cacggagata gcgacaatgg cgtgcagcgc atgcgcgcct
     6661 acgatcaggt agaaaaacgc gccgtgcgcg ccggaggtca tcgtcaggcc ttcgcgcagc
     6721 aaagcgaccc actcgactcc ctggaacaat acgaatgcaa ttcctgtggc aatcgcgcct
     6781 gccaagtacg ggagcgcttt ggcgtagctg tcacgcgagc gccggttcgc tacgtacaat
     6841 aaggcgccgc tgagaagcag gatcgcggtg ttgattgcgg tgcgctctac aggcaggcgc
     6901 ggttgcccta cgggaggcca cacgccgccg aggacagttg cacgcgcgat cgtgtgcgcg
     6961 ctgatcagtc ctgcaaaaaa catgatttcc atgacgacaa aaataatcat cgccaggacg
     7021 gagttgggca gacggtttcc tgcgttgggc gcggacccgg ccattcgtga ttccatcgct
     7081 gttcctgagc tactcatgtt ttaccggtgc gtttggtgcc gaccgcgctt gtacgtcgat
     7141 cggattcttc gtccagtgct cgccgctttg cttcatcacg tccggggcta cacctgcaaa
     7201 cagaatgcag atcaagagca ggcagacgac caggagatag ggcacccagc gcttttcaac
     7261 gttcacgtgc atgaaatttt tagcgacgag atatgctttg accaatgcaa ttccgaacgc
     7321 ggcgatgagc acgacttctc gcacgtgtga catcgatccc agcacgctga cggcaagcag
     7381 cacgacgaga atggcccaga tctttacgta attaggatga tgaacgtgtg attcagtggt
     7441 cataatttct caccagtatt tctccgcgat atgtttcatc gcatctcatc cttgcgcggc
     7501 taccgcgcga tatacagcag cgggaatagg aaaatccaaa cgatatcgac aaagtgccag
     7561 tacagcccga tacattcgac gcgttgcaat tcctgatttt tcgccgcgtc cgacgccacg
     7621 atcagcatga tgatcattcc ggccagcacg tgcaggccgt gcaatccggt gagcacataa
     7681 tagaaaggcc aaaaaccgcc tgaggacagc acgaaccctt catgaatgtc tttcgtccat
     7741 tcaaaacctt tgatggtcag gaaaagcagg ccgccaagaa cggtgaggcg aagaagcttc
     7801 gcggccctca gtccatccag cgcatccgca gccttgtgtg cggatacggc gcataaactc
     7861 gacgtgagca gcactagcgt attggatgca ccggcccaag cgttcgtgtg tgaggcggct
     7921 tcggcccatt ccggatgacc cagtcggtgc atgatgtacg cggcgagcaa gccgccgaag
     7981 atcacgattt ccgaggcgat cacccaccag gtaattaatc gcgctgtggg aataccggtg
     8041 acgcttctcg tattggcaat cggtttggcg cttgcactca taattctttg gctccgagaa
     8101 attattcggg aacgctctgc ggtgtgaaat catccgcccg gcccggcaaa ccatattcgt
     8161 aagggccgcg gtacacggtc ggcagttcgg caaagttacc gtgcggtggc ggtgaaggaa
     8221 cggtccattc gagcgtggcg gatctccatg gattcgctcc cgccttttta ccgtggcgca
     8281 ggctccagat aaaattgaac aaaaagacaa actggaaaga gagcatcacg agcagcgcga
     8341 tcgtggaagt tgtgcgcaga tgctgcagcg ctgggcgcga ttgctcggca aattgtgcgt
     8401 agctgtatac gcggcgctgg tcgcccgcgg tgccggtgat gaacagcgga ataaaaatca
     8461 cgttaaaggg aatcaccgtg ccccagaaat gaattttccc gagcgtttca ttcagcatgg
     8521 ttccgaacat tttcggatac cagaatgtga ttcccgcaaa caccgaaata atcgcaatgg
     8581 gaacgaacgt gtagtggaaa tgcgcgatga cgaaatacgt atcgtgaaga taaatatccg
     8641 tgccggacga gccgaggaaa attccggtga cgccgccgat catgaattcc cctacgaatc
     8701 caagcgccca gagcatggga gtatccagag tgatcgaacc gccgtaaagc gtggcgatgt
     8761 acaggaacac tatttccgtg atgggtacgg aaatcatcat cgtggtaatg gaaaatatat
     8821 tggccatgcg cggatcaatg cctgcgacga attgatgatg cgcccatacg acgaagctca
     8881 ggaagccaac ggcccaggtg gtgtacaaaa ctgttttgta tccaaaaatt ttcttgcgcg
     8941 caaacaccgc gatgatttcg gcgacaattc ccatggctgg aagcagcaca acgtaaactt
     9001 ccggatgccc gaaaaaccag aaaagatgct gccagaggag tggatcgccg ccgcgtgtcg
     9061 gatcgtaaaa tcccgtggac atcgtctggt cgaatagcaa catgaccgcc ccggcgatca
     9121 acgggcctgc ggaagccatg aagattatgc tggcgaggac gatcatccag atgacgagcg
     9181 ggatgtcgta cattttcatg cctggcgcgc ggctgttcat tgtagtgacc gtgaagttga
     9241 tgccgcccag gaggaacgcc acaaattcca gaacaactgc aagcagccac agcacggagc
     9301 cgagcggcgt cagattgtaa gcgccatttg cggaaagtgg cggatatgcg gtccatgcgc
     9361 cgccaaacgc gccaccctta acgagaaaag aggcgaggag gacaatcgcg ctgattagaa
     9421 aaatttggta cgaaaggcga ttgatgcgcg gaaacgccat atcgtcgcag ccgaccatca
     9481 acggaatcag gaaatttccg aacgcagcga tgagcaccgg catggccacc cagaaaatca
     9541 tgatgctgcc atgcatggtc acgagcgcgt tgtacctgcc gggcgttacg aatccatagc
     9601 ccggcacatt ctgattgggg aatccgagtt gcatgcggaa gacataggcg aagaacgcgc
     9661 caatcatggc catggccatg cccgtaaaca aatactgcat ggcaatgact ttgtgatcgg
     9721 tggaccagaa atatttcttg aatattcccg gttcaacgcg ttcaagatgc gtgttcattg
     9781 cgacaggcgt gctcatcgcc gcacctccgc cgtggtcatc gcaccgtccg atgttggcga
     9841 agtgtaaggc aatccggcta cggctggatt tgtgttacgc atcagccacg ccgcgtgatc
     9901 ctgtggcgtc tcgacaataa ttcgtccggc catcaagcca tgtccaaggc cgcacatctg
     9961 cgtacattga atgtcatagg tgcccacttt attggggcgg aaccagccgg tgatggtgcg
    10021 gccgggaaga gcgtcttgct tgagtcgaaa cgctggaact gcgaagctgt gcaccacatc
    10081 gcgcgacatc agatcaaaat ggtaagtcgt atttactttg acgtgcagtt cgccgacgga
    10141 gcggatatcg tccgctgtat ccatcacgcc atccggaccc gcctgctgaa aactccaggc
    10201 ccattgctgg ccaatgacgc gtatggtttc atccgcggcg ggcatattct gcttgatgtt
    10261 gtaccagacc gaaattgcgc caacgatgat tacgacatcg caaaccagaa ccagaaggtg
    10321 agggatggtg atccagcgct tcaaatgttt ttcatcgccg gtgatgtact gggtggggcg
    10381 gccatctttg gcgcgaaatt taaagatcag cacaaacatc atggtttcgg cagcgatcag
    10441 ccaaaggcca acgatcaccc caattaaaat aaacaggtga tcgatgctgt gcgcgtaagt
    10501 ggatgcagcc gataaatacc gctcgtacat ggaagctcca actacctaat caggatgatg
    10561 acacccaaaa taaaagtgat cgaaccggct aaaataatct gaaagacgat tttggccact
    10621 tgttccgcgg atgcttccgt gggatccata agaaagtgtt ctccgccgtt caggtttaac
    10681 gtttcagcgt gcccacatag gcgacaacat tgcgaatcgc atcttcattg gccaagccgc
    10741 gcgccatcgg ccgcattaac cggcctccgg agtccttggg actcgtgccg cgcgccccac
    10801 ttctgaattt tctgagttcc gtggcgaggt accagtcatc cactccggca agaggcggcg
    10861 cttttaaatc ctcattgcct gcgccattgt cgccatggca agcagcgcac gtcgcgaaaa
    10921 tcgcttcgcc agctttcggg tcgccaggga gtgtggcggc gtggcgaacg ggaggcatcg
    10981 attcgacgta gtgtgccacg gacttcacgt catcttcgga agcgaggctg agcgccatcg
    11041 ggcgcatgcg catgccttcc atgtcggtga attgcatgcc gcgcacaccg ccgcgaaatt
    11101 tgtccagttc ttcctcgacg taccactctt tcatccccgc aatgttgggc gcgccgacgg
    11161 tggcattccc ggatccgtcg gtactgtgac acgggacgca tgtctgaaaa acttccttgc
    11221 cgcgagtcgc agcatccgtg ctgccccaac cgcatccgga aagatgaagt gcgcagcccg
    11281 caaccaacag aaaaaatacc caagcggaaa atcgatttgg gacgagtgtt tgagttttgc
    11341 tcattttaag ttaagtcgca aaggacttcc caaggcgcgg atactagcag ttgataaaaa
    11401 tgaaaacaag cacaacctgc cttatacgcg cgtttaaatt cagcaattta acgaggacaa
    11461 atgaaaaagc attccacttt ttcaagccga aatctgtgac ggatgtcact tgcaaaatta
    11521 tttttgtgcg acagcggtgc gctgtagacg ttcaccgagg cgagtatgtt cctcgctgat
    11581 ttcttcgggc aaacgatcgc caaatatctc aaagaaattt ccagtgtcgg catgttcctt
    11641 gacccaatcc gcaggatcga cgcgcagcaa ttcgttcatc gtatccttgg agacgttgat
    11701 gccgtcgagc gtaatcgcat tcgtggtggg aacggctccg atcggagttt ccgtcgccgc
    11761 gccttttcct tcgatccgct cgagcatcca cttcagaacg cgcgcatttt cgccgtagcc
    11821 gggccacagg aattttccgt ccgcgccctt gcggaaccag tttacgtgga aaattttcgg
    11881 aggatgagga atgtttctgc ccatttccag ccagtgtccg aaataatcgg ccatgttata
    11941 gccgcagaag ggaagcatgg ccatcggatc gcgccgcgta actccgacgg ctccggtgat
    12001 cgcggctgtc gtctcggatg ccatggtcga agcgacgtat acgccatgct gccagttcat
    12061 cgcctcgtag acgagcggag cgaggcgtgc gcgacgtccg ccgaaaataa atgcggagat
    12121 gggcactccc gcgggatctt cccatcgggg agaaatggac ggcgcctggc ccgcgggcac
    12181 ggtgtaacgg gaattcgcct gcgctgcggg gccattcgac ggattccact ggcgcccctg
    12241 ccaatcgagc atatctgacg ggatgttgtc gctcataccc tcccaccagg gttcatgctg
    12301 cggagtcatc gccacattcg tgaaaattgt atttttgcgc agcgctgaca tcacattggg
    12361 attcgtcttg tcattcgtgc caggagctac gccaaaaaaa cccgcttcag gattgatggc
    12421 gtgcaggtag ccatccgcgc cgacctgcat ccatgagatg tcgtcgccca ccgtccacac
    12481 gcggtagcct tccttttcca gggcagaaac catcatgcac aaattggttt tgccgcacgc
    12541 gctggggaaa gcggccgcca tgtacgttac ttttccatca ggagattcga gtccgaggat
    12601 gagcatatgt tccgccatcc aaccctgctc tcgcgccatg gaactggcaa tgcgcaggca
    12661 aagcacttct tgccgaggag ggcgttgccg ccgtaacccg agccaatact ccaaattaat
    12721 ttttcttccg gaaaatgagc gatgaggcgg cgatccggac tcaagtcgcc aagggaatgc
    12781 aggcccggaa caaagtcaga agaattgcca agacgatcca gagccacttt gcccatacgg
    12841 gacatgatgc gcatgctggc aacgacgtag gcgctatccg tcagttccac gccgatgcgg
    12901 ctctgcggag aagcggcagg tcccatgatg tagggaacga cgtacatcgt gcggcctttc
    12961 atcgagcctt caaacaacga gcccatgtgt ttcttggctt cggcgggagc catccagttg
    13021 ttcgtggggc cggcttcatc gcggtctttt gagcagatgt acgttacttt ttccgtgcga
    13081 gccacgtcgc tggggtcgct gcggtgcaga atgctgccgg gatatgtttt ttcgttcaga
    13141 ggaaatgtct ggccctcgcg aactaattga tctttgatgc gggcatcttc ttcttctgaa
    13201 ccgtcgcacc actgaatttg cgacgggcgg gtcatcttgg caacagattc gacccattgt
    13261 tcaaggggcg tagacacgaa tcctcctccg aatttatccg gcgtgggtgc gtaatatttc
    13321 cgtgcggaaa gaaaccgcgc agctattcac gaagtgaaaa cacaccgaaa caaaaagttt
    13381 aaggcacgga tgaatcaaac gcaaggcgcc ggcaagaaag tttgccccat gaaggcaata
    13441 tttttcggca tttgcgccca agctttttcg taaatcgttg tgtaatacaa gcttaagtat
    13501 gttagtgcag tgggtaaata taatcttaat cccgcttccc acagggataa gatagctgcg
    13561 cgtgcagcct attgaaattg cacggaagcc attcaggcac tcttccaagc atccactcac
    13621 tcgaagaaga tatatgcgga ttgaacttta gggtcggatc ccggccgctt cgctatttgc
    13681 gtgaagtgtt gggcctcgaa ggctgccgga aaaattcgat tggaaataag agccaaaaat
    13741 ttcttgtcgt ttggattgcc cttgccgaca gggagatgat tatttttctc gcgaagttcc
    13801 ggagaggcga tcctccggcc tattcttagc gacacgtctt atggtttcca cttctttgga
    13861 ttccagtgga agtatttttt gcgcggtctt ctccgcaatc acggcacgaa tttttggaag
    13921 agcggcgcgc gcggccgctt ctcctgcggc aagtaattcg ctggtccgtt caaaatcgtc
    13981 ccaaagaaaa tcttttacca ccggctcgat cgtaacgtcg gctgtgcgtc tccactccag
    14041 atcggcgtgg cgctgcagaa tggcgaaaga aaggccgatg acgtccacga tgctggaagg
    14101 cttgcgattg tttgcagctt cgaggaatac ggcaatcact aaatccgcac cgatgctgcg
    14161 tgccgcttct accggaacgg gagcggcaag aaatccgtcc acgagcgtgc ggccatcgat
    14221 ttcgactgga ataaacatcc ccggataggc acacgatgca cgcagcgcag ggcccaacgg
    14281 cccgtgcgaa aagtagacgg cctcgcccgt ggcaaggtca gtcgcggcga tggccagtgg
    14341 aatgcgcaaa tcttcaaatg tgctcacgcc cagatatttg cgcgggtaat gttccagttt
    14401 ttggttggag gccaggccca tccaggaaag tttccagttg ccaaaatctt tgaatcgagt
    14461 tgcctgcgcc tggcgttcaa tttctggaat cgtatggccg ctggcaaacg aaatggccat
    14521 caaggcgcct gcgcttgtgc ctgtcagtaa atcgacagga attttttcct cttcgaggac
    14581 tcgcagaacc cccaaatggg caatgccgcg cgcgaatcca ccgccgaggg cgaccccaat
    14641 gcggggacgt tccacttgtg gaggaggtgg ttcggtgtaa gcaaactcgc gaacacgccc
    14701 gaccgcgctt tgcagccaat tgataacttt ctttttggga cgcttggcca tggatgggtt
    14761 tagttaaagt tacctctaat tgagacgcac ggcatttctc gttgaccgcg cattgtcgct
    14821 tctgtaacct gcactggcac ctgtaccaat ccgctccgcg aggattggca gggcggctgc
    14881 tccggctatt ttctcagtac ccttatcgta agcaaattga cccactgtcg tgaagggctt
    14941 aacgtaacag acatgtcaaa aaaaatgatc gaaaaagttg tggtgctggg cgcgggaacg
    15001 atgggtgcac gaattgccgc gcatattgcc aacgcgggcg tgtggtgcgc gctgctggac
    15061 attgcgccca cggagctttc cgctgacgag cagaagcgcg gtttgaccct cgccagcatg
    15121 gatgttcgga accggattgt gcgcacgggt cttgccgccg cggtgaaatc caggcctgcc
    15181 gcatttttca agcgaggcgt ggagagccgc attatcactg gaaattttga ggatgattta
    15241 aaactttgcg cggaggccga ctggatcatc gaagtggttg ccgaaaatct ggaaatcaag
    15301 cgcgctctcc tttcgcgtgt agcggcagtt cgcaagccgg gaacgattgt tacgacgaat
    15361 acatcaggac ttcctgttcg cagcattgcg gaagggatgc cggaggaatg gcaacagcac
    15421 tgggcaggca ctcacttttt taatccgccg cgctatttga aacttgtgga aattattccg
    15481 ggaacggcca cgcttcctga agtgatcaaa atgctagctg gtttttgcga tcacaaactt
    15541 ggcaaaggcg ttgtggtcgc gaaagacacg ccgaatttta ttgccaatcg tattggaacg
    15601 ttttccatgc tgaatgcaat tcgcctgatg ggcgaacttg gcatgacgtt tgaggaagtc
    15661 gatgcttgca cggggccggc actcggttgg ccgaagtccg caacgtttcg tctggcggac
    15721 attgttggaa tcgatgtgtt gctgcacgtg attcgcaata ttcacgaaaa tattccaaac
    15781 gatgagtccc gcgaactcta caaagttcca gcgctcattg aggagatggc gaagcgcgga
    15841 tggattggcg acaaaacggg aagcggcttt tacaagcgtg taaaaaaagc gggaggagaa
    15901 tcggaaatcc tcacccttga tcctgtgaaa atggaatacc gcccgcagca gaaagcgcgc
    15961 tttgcttcca tcgaggcagg aaaaggcatt gagaacactc gcgagcgtct gcgcatgctg
    16021 tgcggccctg cactcgaagg aaaagaaggc gacaaggcgc agaaatttat ctggggcgtg
    16081 ctttccggaa tgtgcctgta tgccgcgagg cgcgtgccgg aaatttcgga ttcgattgtt
    16141 gacgtcgatc acgctatgcg ctggggattt ggttgggagc ttgggccatt tgagatttgg
    16201 gatggcattg gcgtcgagcc gatggcgaac cagcttgaga aagagggcaa cgcgctgctg
    16261 ccgctagtta ccgatctttt agcttccggg aaaaaatcct tctaccagtc ggcacaagga
    16321 gagacatctt atttcgacat tgccggaaaa acttacaaac ctgcgccgca gcccgaggga
    16381 attattattt tgaagtcgct gaaggagcgc gcgcgagaga tcgagaaaaa ttccggtacg
    16441 agtctgatcg atctgggcga cggcgtgatc tgctgcgaat ttcattccaa gatgaacgcc
    16501 attggcggcg acctactgca aatgattcac aaagggcttg cgcgcctgcg caccgatttc
    16561 gacgccatgg tgatcgccaa tcagggcgag aatttttccg caggcgcgaa cttgatgctc
    16621 gtgttgatga ccgcgcagga gcaggagtgg gacgatttgc atgtgatggt gcgccagttt
    16681 cagaacacta cgctggcgct taagcattcg ccgaagcctg tcgtggttgc gccacagggc
    16741 ctggcgctgg gcggcgggtg cgaagtggtg ctgcatggga cgcgcgtcca cgcagcggcg
    16801 gaagcttacg ttggcctcgt ggaagtgggc gtcgggctta tcccggctgg cggcggttcc
    16861 aaggaaatga ttattcgcgc gaatgagcgc gccgagggcg aagaagatct cgacctgttc
    16921 catgcgctca agcctgtgtt tgaaaatatt gcgatggcca aggtttcaac gagcggggaa
    16981 gaggcgcgag acctgggcta cctgcgccgc gcggatctgg tggcgatgaa tctggatcgc
    17041 ctcgttgctg acgccaaaga aaccgcgctg ggactggtcc gcagcgaatg gcgccaaacc
    17101 gcgcccgcgc cgaatgagcc gtcgatccgc gtgctgggcc aggaattcag cgctgccgcg
    17161 aagcttgccg tgcacatgat gctgcgtggc gaatatatat cggaatacga tgcattcgta
    17221 gcgcgcaaac tcggccacat tcttgccgga ggcgctctca cttccgcgca gttcgtgagc
    17281 gagcaatatc tcctcgacct cgagcgcgaa gccttcgttt ctctctgcgg cgaacgcaaa
    17341 acgcaggaac gtatcgcgca tacccttaaa accggcaagc cgctacgcaa ttaaaattgt
    17401 ccatcaaaaa tcgcctggac atggctcaga attggtccag gagttccagg tagcgttcgc
    17461 gtgaaattcg ggcagtgcgc tggacattct tgatgatgaa cacagggagt tggtcataac
    17521 gtgggaccac agccggtcgt tcaataccag gcttcgtgaa aaccatatgg tctcctcttt
    17581 cgcgcgagag tcaaatcctt caagttctaa aatgcgcaca agtttgcgat agtggatggg
    17641 gctgatacga ggcatgcaaa tattcttagg aagcgagtgg aactgctagc cgtaacttgg
    17701 tgcgagcaac aagccgacga gggcggtaac tatggccgcg cttctcaaaa ccggattctg
    17761 cgagaatcgc gtcgagtatc cctttttggg cgcactcctc caagaataac gtcactgcct
    17821 cacgcaaacg cgcgcgagct tcggcgatgg aatcgccgca acttgacaca tcgagttccg
    17881 gagagtagct cacgaaagta ggcccctcct tccaaacgtg ggcggtaaag tcgatctctg
    17941 gaagtcgagt tgttttctta gataagattg gcaccatatt gtcctaattc tagcctcttc
    18001 caaagcgaat tcaaagccct gtcaaccctg ggactacaaa tcatcgtcgg cgaggcggcg
    18061 gtcgtggccg ttggcttcga gccagtggta gaggtgaacc tggtagaggt aggaggcgac
    18121 gatttcctcg ccgagttcgt gggtttcgac gcacaggaac gggacgcgca ggcctgcgag
    18181 cgtggcgatc agagactgcg tgatttgatt tggatttgcg gggctgtgct cgtaacggga
    18241 tttcacctgg cttagcgcag cggtgatcac cagcaagcgg tcgggaaatg cgctcatgcg
    18301 gcgcaagcgc gcgacgaaga cggaacgttc catgtggaac gagcacacca ggtcgctcag
    18361 gtccttccgc tcgacgacgc agatatcttc aagcccggca acggagtaat cgccgatttc
    18421 caaaggcctt ttttcgatgc cggaaaacca ccctgaaaag cgtgcgaatg aaaatggatt
    18481 ttgctcgcgc gtgtcgacca caatggctgg cttcggagtg cggagttgcg tgccgccgcg
    18541 ttcggcaatc acgggaacga ttggatgagc caccggcagg gcggtcatct gccattcgag
    18601 tggtgaggtg atcagtgtgc ggcggcgtcg catggaatcg atgatcgcta gagcatttct
    18661 aatttaagta tgaacatctg tgcgataatg ctcctgcctt tcgtaggagg agcggatgca
    18721 agcgtattcg caggatttac gggaacgcgt gttgcgggcg ctggaacgtg gggatggccc
    18781 tgtggaga
//
LOCUS       DASZMB010000192         3718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1004404, whole genome shotgun sequence.
ACCESSION   DASZMB010000192 DASZMB010000000
VERSION     DASZMB010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004404"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VG272_08780"
     CDS             complement(<1..449)
                     /locus_tag="VG272_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13815.1"
                     /translation="MLIVLFWIGLAGVPTNIGRWGKAFVFFVHYINLEIFRLGISVVG
                     LLILFVFFLLPYLKPKMQIELIPSAGASSEVMLRVLNHGNKREFQAQCTPIALRNSPN
                     ELRRGTFDLKWEHTFDRIVSIGNNASCNLVIARVQNEHRNGFSEMEIV"
     gene            complement(827..2719)
                     /locus_tag="VG272_08785"
     CDS             complement(827..2719)
                     /locus_tag="VG272_08785"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWF13816.1"
                     /translation="MRLELSFAKVYSVVALLVVLCFSIPASAAQNSAPSASAPAASAP
                     GEDVYQKRCARCHDQEGSRAPSKDALHKLSATRILRTLDFGLMMAVAYPINRADREAV
                     ANYLGTKAEEAPLPASAFCAANVGILSGPSKDSWAGWSPDASNARYQSAENAGMAAGQ
                     VRHLKLKWALGFPGDVTAFAAPSIVNGTLFVGSASGAVEAVNASTGCIHWVYQANGPV
                     RAAILPVQNGSETSLLFTDLIGWAYVVDAKSGKLIWKKQIEDHDAARLTGSSVALNGI
                     VYVPAASWEETRSLDPQYVCCTFRGSITALRIRDGSVVWKTYMMDPPKRTGTNSVGSA
                     QWGPSGAPIWSAPTIDTKRELLYIGTGDNYSVPATGTSDAVMALDLKTGRIVWTTQVL
                     ANDAYTSACRIKGVNCPPPNGPDFDFGSSPILVRAPNGKDIIVAGQKSGVVYGFDPDA
                     KGKMLWQTRVGKGGLNGGVQWGMASDGQKVYAAVSDVSGVMNTAGPVGGATFDPAKGG
                     GLTALHLEDGSKAWYAPSHPCDPPRPGCSPGQSAALTLIQGVVFSPGLDGHLRAFSTE
                     DGEMLWDFNTTTQTYSTVNGVPAKGGSIDGAGPVVAGGMVFVNSGYPRNGGMPGNVLL
                     AFAPEE"
     gene            complement(2916..>3718)
                     /gene="lipA"
                     /locus_tag="VG272_08790"
     CDS             complement(2916..>3718)
                     /gene="lipA"
                     /locus_tag="VG272_08790"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HWF13817.1"
                     /translation="LKRLMRTLELNTVCESARCPNMGECWEHGTATFMILGNVCTRAC
                     GFCAVPSGKPAGPPDEQEPERVAEAAARMALRYAVVTSVNRDDQPDGGAGIFARTINE
                     IRKRLPACKVEVLIPDFRGDWNALETVITARPDVLNHNTETVPRLYHEVRKGAIYARS
                     LELLRRAKSLAPEMPTKTGLMLGLGEEREEVLAAMRDLASQGTNILTLGQYMQPTREH
                     LPVVRYVHPDEFAEYKKLGEEMGFAHVESGPLVRSSYHAFEQEAAAHT"
BASE COUNT          895 a         1109 c          887 g          827 t
ORIGIN      
        1 acaatctcca tttcggaaaa cccgtttcta tgttcgttct gcactctggc aatgacaaga
       61 ttacaagaag cattatttcc aattgaaacg attcggtcaa aagtgtgctc ccacttcaaa
      121 tcaaacgtcc cgcgccgaag ttcatttgga ctatttcgca gcgcaatcgg ggtacattgc
      181 gcctggaatt cccttttgtt tccgtgattt agaactcgta gcataacctc agaacttgcc
      241 cctgctgatg gaattagttc aatctgcatt tttggcttta ggtaagggag taaaaaaaag
      301 acaaataaaa tcagaagacc gactactgaa atacctagcc tgaatatctc taggtttatg
      361 taatgcacga aaaatacaaa tgcttttccc cagcgcccaa tattagtggg gactccggca
      421 agtccaatcc aaaacaaaac tatcagcatg attccccaaa ctctcttgac cccgctagac
      481 ataaacaccc ccgaaaacgc ttttgagtat ccgtattggc atacccaagc cattcacggg
      541 taagtttaag gcacctaacc atttgaatca agtgaatagt atcggttatg taaatatgga
      601 ttaatacgat ttctcggcct taagtcgctc ggacttcaga atctgtctga gaactgcaga
      661 atccttgtgg gtcgtggctt tagccacgac ataagttctg caaaatgaat tcggctttag
      721 ccgctgaagt ttcaattttg cagttctcac acagactctt caacctgtgg aatttaaacc
      781 ttgcgaggac taaaccccac aggctgaagc ctatgccacc aaaaaactac tcttccggcg
      841 caaatgccag cagcacattg cccggcattc caccattgcg cggatatcca gaattcacaa
      901 aaaccatgcc gccagcaacc accggccccg cgccatcgat cgatcctccc ttcgcgggaa
      961 cgccgttcac tgtggaatac gtttgcgtag ttgtattgaa atcccagagc atctcgccgt
     1021 cctctgtact gaacgctcgc aagtgcccat cgagccctgg agaaaacacc acgccctgaa
     1081 tcagagtgag cgcagcagac tgcccaggac tgcaaccggg acgcggcgga tcgcatggat
     1141 gactcggcgc ataccaggcc ttcgagccgt cttcgagatg cagcgccgtc aaccctccgc
     1201 ccttcgcggg gtcgaacgtc gcgccgccca cgggccccgc cgtattcatc acgccggaaa
     1261 catcggaaac agccgcgtaa actttttgcc cgtcgctggc catgccccat tgcacgccgc
     1321 cgttgaggcc gccctttccg acgcgcgttt gccaaagcat ttttcctttt gcatccggat
     1381 cgaatccgta tacgacgccg gatttttgtc ccgcaacaat aatgtctttt ccgttcggcg
     1441 cgcgaaccag aatcggcgat gaaccaaaat caaaatctgg cccattcggc ggagggcaat
     1501 tcactccctt aatgcggcaa gctgaagtgt acgcatcgtt ggcaagcact tgcgtggtcc
     1561 acacgatgcg gcccgttttc agatcaagag ccatcacggc atcgctcgtt cccgttgccg
     1621 gcactgagta gttgtcaccc gttccaatgt agagcaattc tcgtttggta tcgatcgtcg
     1681 gcgcggacca gatcggcgcg cccgacggcc cccactgcgc cgagcccacg ctgttcgtgc
     1741 cggttctttt cggcggatcc atcatgtagg ttttccaaac gacggagccg tcacgaattc
     1801 gcagcgccgt aatactcccg cgaaacgtac agcaaacata ctgtggatcg agcgaacgag
     1861 tttcttccca agacgcagcg ggaacgtaca caattccatt cagcgccacg gacgagcctg
     1921 tcaaccgcgc cgcgtcatga tcctcaattt gttttttcca gatcaacttg ccggacttgg
     1981 catccaccac ataggcccag ccaatcagat ccgtaaacag cagcgatgtt tcagagccat
     2041 tctgcactgg cagaatcgcg gcgcgcaccg ggccattggc ctgatacacc caatgaatgc
     2101 agccggtgct ggcgttcaca gcctcgaccg caccgctcgc gctgcccaca aataaagtgc
     2161 cgttcacaat cgacggcgct gcaaatgccg taacgtcacc cggaaatcca agcgcccatt
     2221 tcaatttcag gtgccgcacc tgccccgccg ccattccggc attctcggca ctttggtatc
     2281 gcgcattgga ggcatcggga ctccatcccg cccaggaatc tttggatgga ccagacagga
     2341 tgcctacatt agcagcgcaa aatgcgctgg ccggcaaagg cgcttcttcg gccttcgtcc
     2401 ccagatagtt cgcaacggct tcacgatccg cgcggttaat cggataagcc accgccatca
     2461 tcaatccgaa atcaagcgtt ctgagaattc gcgtagcgga aagtttatgc aaagcgtcct
     2521 tggaaggtgc gcgggaaccc tcctgatcgt ggcatcgcgc gcagcgcttt tggtaaacat
     2581 cttcgccggg agccgatgca gcgggggccg aagcggaagg cgcggaattt tgcgccgcgc
     2641 tcgcgggaat cgaaaagcat aggaccacta agagcgcgac cacggaataa acttttgcaa
     2701 aactcaattc aagtctcatt gaatttttcc tcgaaggaat cagtgccggg ggaaatctta
     2761 cgacatttca agagacatcg tgaaatcaac gtgcggtctt cgtggcatag gcttcagcct
     2821 gtgggtttta gtctttgcaa ggcctaactt ccacaggctg aagcctatgc cacaaaattc
     2881 caattcagtt tttattctga gatggaagca aatagctacg tatgcgccgc agcttcctgc
     2941 tcaaacgcat gataggagga acgcaccagg ggacccgact caacatgcgc gaaacccatc
     3001 tcttcgccta attttttata ttcggcaaat tcatcgggat ggacgtagcg caccacaggc
     3061 aaatgctctc gcgtgggctg catgtattgg ccaagcgtca aaatgttcgt accctgcgaa
     3121 gccaaatccc gcatggcggc caacacttct tcgcgctctt ctccaaggcc aagcatcagc
     3181 ccggttttcg tgggcatctc aggcgccaag gatttcgcgc ggcgcagcag ttcgagcgat
     3241 cgcgcgtaaa tagcgccctt gcgaacttcg tgataaagcc gaggcacagt ttccgtattg
     3301 tgattcagaa cgtctggccg tgcggtgatt acagtttcca acgcgttcca gtccccacga
     3361 aaatcaggga tcagcacttc gaccttgcaa gccggtagtc tcttgcgaat ttcattaatc
     3421 gtgcgcgcga aaattcccgc accgccatca ggctggtcat ccctattcac agacgtcacc
     3481 accgcgtagc gcaaagccat ccgagcggca gcttcggcca cgcgctcagg ctcctgttca
     3541 tccggcggcc ccgccggctt gcccgaaggc acagcacaaa atccgcatgc tcgcgtgcaa
     3601 acatttccca ggatcatgaa ggttgccgtg ccatgttccc agcattcgcc catgttcggg
     3661 cagcgcgcgc tctcacacac agtattcaat tccagcgtgc gcatcagccg cttcaaat
//
LOCUS       DASZMB010000193        24449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3224032, whole genome shotgun sequence.
ACCESSION   DASZMB010000193 DASZMB010000000
VERSION     DASZMB010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 24449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24449
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3224032"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..714
                     /locus_tag="VG272_08795"
     CDS             319..714
                     /locus_tag="VG272_08795"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWF13818.1"
                     /translation="MIIGDQLRAIREEKKLSQGEIEKRTGLLRCYISRVENGHTVPAV
                     ETLEKFARALEVPLYQLFYDSDKPPALPNILKRKSAGEIAWGSSGKDARMLERFRQLL
                     SRTKEGDRKLLLFMAQKMARGKSAGSSAD"
     gene            complement(745..1962)
                     /locus_tag="VG272_08800"
     CDS             complement(745..1962)
                     /locus_tag="VG272_08800"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF13819.1"
                     /translation="MSVVQDSRPRAARTSSLSDKAQTVAVVCGCIVAFTYSANYTNHA
                     PLAAALMRQFGFNNALAGLLTTGIFATHAGMQIPGGYLVDRLGSKRVLLFALLWVALG
                     NIGMALAGAYWQLLFFKIFSGMGTGVCFVGGARYIHEATAGPRLHVAQGFFGGSIQMG
                     AGFVILGVPRLYLLAGWRATFVVSAAMVLVAAIIWVSASPTVEFGSPPPGRFHEMLLA
                     PQLWFLGLMQMATFGISVVVGSWVVVLLTKVMKVPLTRAGLIGSLVLLLGIVSRPLGG
                     FLRRHMGIRPLFIGSLLMIAIGCSLFLPSFVSLPAALTAVVLIGIGVGLPYAAMFSRA
                     GELFPGRAGAAMGFVNMLGIIMILGGAPLVGHLADLTGSFKTSFAVLAGFSLVTCLVV
                     PWIDREDPARTAH"
     gene            complement(2342..3595)
                     /locus_tag="VG272_08805"
     CDS             complement(2342..3595)
                     /locus_tag="VG272_08805"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF13820.1"
                     /translation="MKREDKTIQDQAAVTNTGTEDLKARARGVAIAMYCVLLVSYILM
                     AADRYLFPVLAPDVRKAFGFSLANTGLLSTIFTLGLGIGGLPTGYLLARYSRKMVLLT
                     GIVIFSSATALTTMVPGFWTMLVCLAAQGIGMSMLATSMFALAASYFSGYRSAAIGSV
                     NFCYGIGGFLGPFLAGKLRQSYNTWHAPMLAFGLFGFVMVAIIAISVRPWFVETRRAV
                     EIKADSGGAASLLNRNTIILTTLSLIHGLGMYGFLGLYPTFLRETLRYSPSDAGKVIG
                     FFGIGALASIPCGWIGDRYSPKLVLSGSLLSIAVLGYLFFQISPTMLTREILTCIYGV
                     TGSAVLYVNLAGYHVKALRRSMSSRGSGMFVTSLYGGAAFGGYLLGWIVGHGGWQLAA
                     EIQMSLFSVIGAVLALALRSSEMSL"
     gene            complement(3669..4685)
                     /locus_tag="VG272_08810"
     CDS             complement(3669..4685)
                     /locus_tag="VG272_08810"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HWF13821.1"
                     /translation="MKAIVLEGPRKLALSDVARPAIGHNEVLVRVTYSGVCGTDLHIY
                     EGAIPVRHPLIMGHEMIGEVVDGGDASLPIGTRVIVDPAMYCGMCLNCREGQYNLCPN
                     GSLVGRDSNGGFADYLVAPRTHVYPLPASIESNVAPLIQVATTCVHGHSRMKIFPGQS
                     VVVMGLGVGGQIHVQVAKSWGAYPVIGITRSAYKRKLAEQLGADITMSSGAESVRAVK
                     EATEGRGADIVIECTGVPSALADAINMVRLGGTILLFGITAATQMTLPFYQLYYKELH
                     VVNTRAATGRDFPPTIDLVSRGVLKLKPLVTHIVPLFDLEKAMGMLVSDEDQRMKIIM
                     ENPR"
     gene            complement(4907..6157)
                     /locus_tag="VG272_08815"
     CDS             complement(4907..6157)
                     /locus_tag="VG272_08815"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13822.1"
                     /translation="MSSTPWKTDKWFVSPWNFNPAVNGDFKFAKKIEFHDVTLRDGEQ
                     QTGVIFTKDDKIRIAEGLAAAGVHRIEAGMPVVSPSDTAAIKEIVKRNLGPKIFAFSR
                     CMVDDVKRAVDCGVKGVVMEVPSSEHIIKYAYKWSMEKAIDLSIESTKYAHEQGLEVV
                     FFPIDFSRAEMNWVLDLILRVANEGHMDALALVDTFGVLSPHSIQYLVRSVKARINKR
                     LEAHFHMDFGMGVANTIMALAEGVEVVHSTVLGLGERAGNVPMEDTAMALLTLYGIDT
                     GLKYDKFYGLSKLVAQLSGHAVPSNRQVVGDSLFKIESGIIASWFENCGVENATELFP
                     FRWDVVGQDPVSVVFGKGSGIDSVKWGLRKLGVQFNEEEAMKCVAAIKDFSLQRKRLL
                     TDAEFRDVVNATLPGRVGSGKAAD"
     gene            complement(6184..7164)
                     /locus_tag="VG272_08820"
     CDS             complement(6184..7164)
                     /locus_tag="VG272_08820"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HWF13823.1"
                     /translation="MASLLVAVADSVFPNLDLARAVVARAGAELRLAAKPTPEEIVAV
                     AKDADAVLVTYAKINADMIGQMKKCRIISRFGIGVDNVDISAATAQGIVVTKVPDYCI
                     DEVSDHAVALLLSLIRKIPASSARAHAGRWEMPAVVPIHRLRGTTLGLVGFGRIPQLV
                     APKAKAFGIRVVASDPFVPQSVMDAAGVGKVEFAELLKVSDYISIHSPLLPETHHLFS
                     TDVFRQMKPGALIVNTSRGPVVDEAALAAALDAKQIGGAALDVLEQEPPTSSPLFGRD
                     NVILTPHTSFYSVESLEELQTKAAEEVVRVLSGQAPRNPVNPEALANAHK"
     gene            complement(7164..8435)
                     /locus_tag="VG272_08825"
     CDS             complement(7164..8435)
                     /locus_tag="VG272_08825"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HWF13824.1"
                     /translation="MDTRFIGQPHSWDPRRVYSQTGADWQMRVDFDRLRKERLAKLRA
                     SMKANDLGALVLFAGANIRYATASYQGNWKYNINIRYCVVTHTTDPVLFETAGSDLHC
                     AEIDLPWMEGKIRPAITWQWAEGAVPYMAGRMAESVMDVLKEAGVTKERIGVDNFDMP
                     ALEAFQKTGLNIVNGWPAVSQARVVKTQDEIELLKQASSIGDAAMWKIKYEWLKPGVR
                     EREIEAKVHEFMLERGCEIIYDIIVASGGNTSPYRRWATDKLIRQGDLVIVDINAVGP
                     SGYFIDFVRCFKCGGAQGMKMTQKEIDLYREVYDSMYAGIEKLRPGMTSADVANAFPE
                     YDDDKFGTVTLQQFAHSIGITLYEGMWMSRAYSLKYPAEIKENMYFAIETFAGHPGLQ
                     QTCRLEENVLVSAKGPVVFTLMEHMEEAMKR"
     gene            8701..9225
                     /locus_tag="VG272_08830"
     CDS             8701..9225
                     /locus_tag="VG272_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13825.1"
                     /translation="MRHSTLVAMGVRWLSRQCSVVLYEFATPADENPDVIGWISGAGS
                     VLIECKLTRSDFLRDAAKPVRKNPRAGMGQRRYYLCPPELIQVKDLPPKWGLLWANKG
                     QIIVKREARGHSERNLIAEVQFLNSMLRRAQIRIGTRPLSEWLRGENRFEAKRGISPT
                     REPIVRKAKSSVSG"
     gene            complement(9239..10330)
                     /locus_tag="VG272_08835"
     CDS             complement(9239..10330)
                     /locus_tag="VG272_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13826.1"
                     /translation="MQLRGLFRLLFLYDVAESIDLSKLRNLLGARVGSSGQAFPRRTP
                     GYVRFEDPPVLEMADCISVAAGGNVACPVKYYGFGAIVIQVEVPFDCDMTTLVEQSSR
                     WMDAADVEPLVRELVLRRLEQIRPAVTRATEDWLQENYLVVEIHKILDERGMQPAADE
                     LITSYGGELVQLIRGETTMLARHSIDEALQSSLSYYPSDLALVGSHGAVVVDGPEDAA
                     AVTQILEYAKMQLLEFRYYDRFLTRVLADFYLALERKRNILTSRLTLPRESQHFNTIR
                     LDVMELTERIDNAIKFVSDIYYARVYRLAATRMGVEDYRNLVDQKLDTFGELYDSMVD
                     RFNETRSFVLELLVAILAVLDVIFLFRDR"
     gene            complement(10511..10744)
                     /locus_tag="VG272_08840"
     CDS             complement(10511..10744)
                     /locus_tag="VG272_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13827.1"
                     /translation="MLVHIFRGAGRVFGFTEDSNGTNLPSQFGPWTAFKSLDLNRDGK
                     STPGVNTSECLDDIEKYGLHLTDAHVRITDRVA"
     gene            10903..10978
                     /locus_tag="VG272_08845"
     tRNA            10903..10978
                     /locus_tag="VG272_08845"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10936..10938,aa:His,seq:gtg)
     gene            complement(11219..11617)
                     /locus_tag="VG272_08850"
     CDS             complement(11219..11617)
                     /locus_tag="VG272_08850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWF13828.1"
                     /translation="MNALRVLIVDDHDITRRAIRSFLVSYPELQICGEAVDGLEAVDK
                     AKSLRPDVILMDISMPRMNGLEATKLIQKENLNTKVIIVSQNDPAVVGLQAREAGAAG
                     YVSKKDIGQHLLPILRSVTDQPAGWPGLYI"
     gene            12010..13113
                     /locus_tag="VG272_08855"
     CDS             12010..13113
                     /locus_tag="VG272_08855"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF13829.1"
                     /translation="MKHCNAFGNGSGLEVRSDEPRLSRRKVMGMISAVGAGMALAPRS
                     FAQSVAAGPRIIDTHHHIFPPAFTARNLKRMTDDSPTFPGSFYTNWTPRYSLEQMDQN
                     GVATAIGSMTSPGVWFGNNEEGRRNARDCNEYGAKLVQDFPGRFGMWGAIPLPDIEGS
                     LREIEYIYDHLNLDGIGLMTSYDDGKLLGHPNFAPVLEELNHRKAVIFVHPTISCCAM
                     PIRHLNRVQIDFPTDTTRTITDLIFSGSLIRYPDIRWIFSHGGGTVLMLTPRLAGGNL
                     TPEERAVTIPNGFEHELQKLYYDIASVALSPIPMAAVLKGIPKEHLLFGSDIPFFTIE
                     RIATAVNKFEVPSADLRLLQRENALQLLPRLKA"
     gene            complement(13412..14353)
                     /locus_tag="VG272_08860"
     CDS             complement(13412..14353)
                     /locus_tag="VG272_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWF13830.1"
                     /translation="MSIVNKWSAARITFFLILPVALVTPSLSRAQLKQPLLSEDNLKR
                     VSDHVYVIEGFPNVAIIVGSRATMVVDTGLGQKNGATVMRAEQKLAKGPVLYLTTTHY
                     HSEHTTGEQAFPASTIIIRNSAQQDEMNKAVAAHIEVFKKMSAQNLELLQDVKFRVPD
                     VVFDREAKVDLGGVTARLFWLGPAHTKGDELIFVEEDSVLIPGDIVQKDIFPIMPNPD
                     ASVKGWLSILDNVEALHPKFIVPDHGPAIVDASQIATQRAYLLALQSRALELKKQGVS
                     VEDAGKTITAELKAKNPDWPNPNNVAGEVARVYEQSR"
     gene            complement(14430..14777)
                     /locus_tag="VG272_08865"
     CDS             complement(14430..14777)
                     /locus_tag="VG272_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13831.1"
                     /translation="MSLTFIDTNKIPHMKASGAGEFAEILNNQLCGAKNVVGSLRWLN
                     AGEKLDVRSDKDTHQLVYLMDGEGTITMDSKDHGVSKGAGVYVGPSDSVTIKHVGTAP
                     LKLFQLVVPHLPA"
     gene            complement(14863..15270)
                     /locus_tag="VG272_08870"
     CDS             complement(14863..15270)
                     /locus_tag="VG272_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HWF13832.1"
                     /translation="MAYIFNQDQLPRLLSNGRERVFFVNKELTKIDDMLAGIMYYKQG
                     AASPYHMHKNCEHFYFIMDGKATVQSEEGTREIGPGMMVFIPAEEKHRLHAVEPLFYF
                     EFQAPNRFQTTILEGTPDDLKWDRVDGGIWMQS"
     gene            complement(15625..17283)
                     /locus_tag="VG272_08875"
     CDS             complement(15625..17283)
                     /locus_tag="VG272_08875"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWF13833.1"
                     /translation="MRMTRLRVRVSALRTIAGIFLCLGTLAISFGTIQKFGGKSLAAS
                     PAAGDDADWTYADHDLNGTRYSPLKQINSGNVKQLAKICSYTFPEKVPSESEPIVSAG
                     VIYATSDHYTVALDAADCRVLWSYQWVPRATDRVHPHRGAAIANGKIIRGTGDDFLIS
                     LDAETGKLLWAKQIANPDEGHFIAMPPLVHGDLIYIGPAGSERAGTGWIGAFKLSDGE
                     QVWKFNIIPQDGEPGADTWGPDPEARKHAGGALWTAQSYDTEKDVLYIAGGNPAPDFY
                     DDARPGINLYTNSMIALDGKTGRLLWYNQFIPHDVDDYDITHVNPIFKVNSQTMIAST
                     GKDGVLRVVDGETHKLVYSSPFTTQLNNEVPLTYTPIKVCPGILGGNEWNSGGYSPKL
                     SLLIIPSNDQWCSMNMKDKEAPSVAKANTGEKSYFGGPIDHGAFSGSRGRLTGFDAAT
                     GKERWRYESPTPLIAGVAITASDLIFTGESGGFFDAVDAQTGKVLLHYDLGDSIQGGV
                     ITYSAHGIQYVAVTSGDGNVIGNKSFPEIKGGNPTVTIFGLPKK"
     gene            17580..18284
                     /locus_tag="VG272_08880"
     CDS             17580..18284
                     /locus_tag="VG272_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13834.1"
                     /translation="MAEPKDPTNLDSKTLVDRRDFLGGATVLAGSSLLGLASAGRADA
                     AEGVADRMPPIPAEKWTEAQKKAAEEITSGPRKDLVGPFIPLLRSPEYMSRLQRVGEY
                     LRYKTNLGSGISEFIILLIARRWTQQFEWYSHEALAAKAGIKAETMKAIAEGHRPTTM
                     TPDEEMVYEFVAELLANQSVSDPTYARILNRFGEQGVIDITGLCGYYTSLGMLMNVAR
                     TPVPPGVTPPLAQFPR"
     gene            18541..19569
                     /locus_tag="VG272_08885"
     CDS             18541..19569
                     /locus_tag="VG272_08885"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HWF13835.1"
                     /translation="MRLITFRHLGQQEIGALADADTKVIRLQAAEELRCGEANPHFQA
                     MLSFLQGGGTSRDAAQQTFEFALAQRPDGIVIARENIELLSPVPRPESIREFMVFEQH
                     VINCTRRFEMPGWRAAVDRFVEKMFGRKATLAYRKAQPWYERPVYYKGNRMNVIGDGA
                     EVTIPLYTQKFDWELEFGIFLCKKGRDISKGKAREFIGGYTIFNDFSARDIQKREMGG
                     RLGPAKGKDFDGGNSIGPVLVTPDEIPDPYNLTMRARINGEEVSFGHTKGMRWKFEDL
                     IAYVSQDETLYPGEFFGSGTGTVPESGSNRCCCGLEMGRFLKAGDTIELEVEKIGKLT
                     NRVVGQSS"
     gene            19624..20961
                     /locus_tag="VG272_08890"
     CDS             19624..20961
                     /locus_tag="VG272_08890"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF13836.1"
                     /translation="MNFKSISIALFLISISISVRAAAQAPDPALMAEIHKIRAIDNHS
                     HPPRVVENGETDDEFDALPCDPLEPTEPNTMTRPENPQYLAAWKALWGYEYTDRSDAH
                     VKELIAKKNNIRQEQGDHYPAWVLDQLGIDIEFANRVAMGRGLDAKHFRWVPFDDALL
                     FPLNNDALAAETPDRKFFFGRENMLLARYRRELGVASMPPSLAEYTSRVITPELERQK
                     NTGAVAIKFEAAYLRSLDFEPASERDAAAIYAKYLHGGVPVKEDYTRLQDYLFRYVAA
                     EAGRLGLPVHIHTGAGCGGYFFLSGSHPLLLESVLNDARLRKTTFVLLHGGSGPFTGE
                     IAPLLMKPNVYADLSEQTWMESPHRLAEVLREWMEWYPEKILFGTDLYPGSGAYDWEE
                     IGWQTSQTGRQALGMALTEMLHSGEITVLRANEIARMVLRENAIKLYHLQDAQ"
     gene            complement(21134..21529)
                     /locus_tag="VG272_08895"
     CDS             complement(21134..21529)
                     /locus_tag="VG272_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13837.1"
                     /translation="MEQYNSNIEKGDFLFMSRRLLPVVLAIMALTTMGVSAWAKNDSS
                     DTITTNLKLTVPTTVGTTQLAAGEYKVIADGSKAKFQQGNKVVAEVPCTLKDSSVKIN
                     ETTFIIDHDKLTEIQVSGKTKSIEFSSGM"
     gene            complement(21706..22179)
                     /locus_tag="VG272_08900"
     CDS             complement(21706..22179)
                     /locus_tag="VG272_08900"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWF13838.1"
                     /translation="MNEDKLRKHLLELLSGRGAHADWKASFRGIPPKLRGVRPSGLPH
                     SLWELLEHMRIAQWDILEFSRDARHVSPDWPTGYWPAAPEPPNAKAWDKSVKMFARDL
                     DAMKKLVSNRKRDLFEKIPHGTGQTILREALLVADHNAYHLGQVVAVRKLLDNWK"
     gene            complement(22203..22925)
                     /locus_tag="VG272_08905"
     CDS             complement(22203..22925)
                     /locus_tag="VG272_08905"
                     /inference="COORDINATES: protein motif:HMM:NF018983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016977 - chitosanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 75 protein"
                     /protein_id="HWF13839.1"
                     /translation="MLLLNFEMRHDGETERDIPIGHLPGTSAFFYESGMTIDADGAPN
                     AYHPDNSGIDDLENAGAPGIWEGLAKDAQGEPFIQGPNDPFPGYYVSETALADRSKAV
                     SDPARYVDASKIPFLVLPGGLARQLGARVGDFAIVFNERNGKSSYAIFGDVGPFDRIG
                     EGSVALAENLGIRSNARNGGTWRGVLYLVFPGSGEGRPRTIEEINDEGQKLLADWETG
                     ISADACGSRKSIPSAQGIQSAN"
     gene            23588..24016
                     /locus_tag="VG272_08910"
     CDS             23588..24016
                     /locus_tag="VG272_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13840.1"
                     /translation="MLVELAAVARREGSSRDIGVYFIFPNHESLPIGERTCSPVAMLD
                     FIAHRNIIHGAGCARSNFPWSSFESTPISERFDPMHDLPSIWNERTNFQTARKQASLS
                     KRILFLSRTKQVFNLWHVIHRPFSYSFAILAILHIGELYT"
     gene            24187..>24449
                     /locus_tag="VG272_08915"
     CDS             24187..>24449
                     /locus_tag="VG272_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13841.1"
                     /translation="MTAIVSVYTPEGFVVGADGLRVDANGVDVTDTAVKIFPIIHPDL
                     IGVHAWAGTTYLFSLGRPLFSFSEEADSLMKELSNAPVQSLSD"
BASE COUNT         5809 a         6492 c         6458 g         5690 t
ORIGIN      
        1 aggctttcat attcgtccat cgcggatctc cttttcgtgc gctttggcgg ctcacgctta
       61 ggagtttccg cgatggactc cagtatctgt caaactctta ctgcctcccc ggcagagccg
      121 ggggaactcc cacgagatta ggcctttggg actctaaaga gatttatttc gtagtgtggc
      181 ctggaatgaa gcaattaagt cactaatttg atacaatgtt cacatagggt acttgttgct
      241 ccgaaagtac taatttgaat tacttagatt tagtagttaa tcaaattatc gtcagaaaaa
      301 acttgaaagg acaaaaaaat gattatcggg gaccagctgc gagctatccg cgaagaaaag
      361 aaactctcgc aaggtgaaat tgaaaagcgt acaggtctgc ttcggtgtta catctcgcga
      421 gtggaaaatg gacatacggt tccagcggtg gagacactgg aaaagtttgc ccgcgctttg
      481 gaagttccgc tgtatcagct tttttatgac agcgacaaac cgcccgctct gccgaacatt
      541 ctcaagcgca agagcgccgg agaaatcgcc tggggaagtt ccggcaagga tgcgaggatg
      601 ctggaaagat ttcgtcagct tctaagccgc acaaaagaag gcgatcgcaa acttcttcta
      661 ttcatggctc agaaaatggc aagaggaaaa agcgcgggaa gctcggccga ttaatttttc
      721 ctgttttgtt ttagcgtttt cagcttaatg cgctgtcctg gccgggtcct cgcggtcgat
      781 ccatggcacc accaagcagg tgaccagtga gaatccggca aggacagcga agctggtctt
      841 gaaagatccc gtcaaatccg ccagatgccc cacaagcggt gcaccaccca aaatcattat
      901 gatcccgagc atgttgacga agcccatggc tgccccggcg cggcccggga agagttcccc
      961 tgcgcgcgaa aacattgcag cataaggaag tcccacgcca attccaatca acactacggc
     1021 tgttaatgcg gcggggagcg atacgaatga tggaaggaac aggctgcatc cgatcgcgat
     1081 cattaacagg ctgcctatga ataatggcct gatgcccatg tgccggcgca gaaagcctcc
     1141 gagtgggcgc gacacaattc cgagcaatag aacaagggag ccaatgagtc ccgcacgcgt
     1201 gaggggaact ttcattactt ttgtcagcag aaccacaacc cacgatccga caaccacgga
     1261 aatgccaaac gtcgccatct gcatgagtcc cagaaaccaa agctggggag caagcagcat
     1321 ttcatggaag cgtcccggtg gcggcgaacc gaactccacg gttggcgatg cagataccca
     1381 aataatggcc gccaccagaa ccatggccgc ggaaacgacg aatgtggcac gccatcccgc
     1441 cagaagatac agccggggca cacccagaat tacaaatccc gctcccatct gaatggagcc
     1501 gccgaaaaat ccttgcgcta cgtgcaaacg cggaccagcc gtggcctcat gaatataacg
     1561 cgcgcctccg acgaagcaga cgcccgtgcc catgccggag aaaattttaa agaaaaggag
     1621 ctgccagtag gctccggcaa gcgccattcc gatattgccc agcgccaccc agagcaacgc
     1681 gaaaagcaag acgcgcttgg aaccgagccg gtcgacaaga tatccacccg gaatttgcat
     1741 tccggcatgc gttgcgaaaa ttccggtggt gagcagcccg gcaagggcat tattaaaacc
     1801 gaactgccgc atcagggcag ccgccagggg cgcgtgattc gtgtagttag ccgaatatgt
     1861 gaatgcgacg atgcacccgc aaacgacggc tactgtttgc gctttgtcgg ataaggagga
     1921 agtgcgcgcg gcgcgaggcc gggaatcctg gacgacgctc atgcgcgggg ccgccgcgat
     1981 ttggtttcat ttaggatggg gcacacgttt tgaaaggtcc ttattacagt tgaaattttc
     2041 atttcagcaa ttttgagatt gcgtttgata ctacactggc tggcgcgatt cgcgccacaa
     2101 agtttcgtgc cagtatattt ggatgacgtc ctgatgctgg aaataatttc gctgcgaaaa
     2161 cttttgaata ctgattcatc cttgcacgga caaaacaccg gtgtagcctg cattttcatt
     2221 ggcgggattt agatgacctg acatttaaac cccgcctctt aagaggcgga ctacagcagc
     2281 gggtcattct tgctaggact gaaccccaca gactgaagcc tgtgctactt aaaggtaaaa
     2341 tttataggga catttcgctg gagcgcagcg ctagcgcaag gactgcgccg atcacggaaa
     2401 acagtgacat ttgaatttcc gcggccagct gccagccgcc gtggccgaca atccatccta
     2461 acaggtaacc gccaaaggct gctccgccgt aaaggctggt gacgaacatt cctgagccgc
     2521 ggctggacat gctgcgccgc agggctttta cgtggtagcc ggccagattc acgtagagga
     2581 cggcgctccc tgtcacacca tagatgcagg tcaaaatttc acgggtcagc atggttggcg
     2641 aaatttggaa aaacaggtac ccgagaaccg caatggaaag cagcgaaccg ctgagcacca
     2701 acttgggtga atagcgatcg ccaatccagc cacaggggat cgaggcgagc gcgccgattc
     2761 caaaaaagcc gatgaccttc ccggcatcgc tgggtgaata gcgcagagtt tccctaagga
     2821 acgtcggata caggcccagg aatccgtaca tgccgagtcc atgaatcaaa ctcaaggtag
     2881 taaggattat tgtgttgcgg ttcagaaggc ttgcagcgcc gccgctgtcc gctttgatct
     2941 ccactgcgcg tcgcgtttcc acgaaccacg ggcgcacgga aatggcgatg atcgccacca
     3001 ttacaaaacc aaataatccg aatgcgagca ttggcgcgtg ccaggtgttg taggactgcc
     3061 gaagctttcc ggcaaggaac ggcccaagaa atccgccaat gccataacaa aaattcacgg
     3121 agccgatggc cgcggagcgg tagccggaaa aataactcgc ggcaagcgcg aacatggacg
     3181 tcgctagcat cgacatgccg atgccctgcg ccgcaaggca tacgagcatg gtccagaagc
     3241 cgggaaccat cgtggtcagc gccgtggctg aagaaaaaat aacgatgccc gtgagcagaa
     3301 ccatttttcg ggagtaacgt gcgagcaggt agcctgttgg aaggccgccg atgccgaggc
     3361 ccagagtaaa aattgtcgag agcagtccgg tattggcgag agaaaaaccg aaggccttgc
     3421 gaacgtcagg ggcgaggacc ggaaaaaggt agcggtccgc agccatgagg atgtaggaaa
     3481 cgagcagcac gcaatacatt gcaatagcca caccgcgcgc gcgcgcctta agatcttccg
     3541 tgcctgtgtt ggttacggct gcttggtcct gaattgtctt atcctcccgc ttcacggaga
     3601 gagtgacatc cggcttactt tcgcttcagg acacaataac gggtcggcaa aaaattgcaa
     3661 ggaatccgtt aacgagggtt ctccataata atcttcatgc gctgatcttc gtcggatacc
     3721 agcatgccca tcgctttttc gagatcgaag agcggcacaa tgtgcgtcac gagaggcttg
     3781 agttttagca ctccgcgcga caccagatca attgtgggcg gaaaatccct gcccgttgcg
     3841 gcgcgcgtgt tcaccacgtg cagttccttg taatacagct gataaaacgg cagggtcatc
     3901 tgagtggccg cggtaattcc gaacaagagt atcgtgccgc ccaggcgcac catattaatc
     3961 gcatcggcca gcgcggaggg cacacccgta cattcaataa caatgtccgc gccgcgaccc
     4021 tcggtggctt ccttgacggc gcgaacggat tccgcgccgc tggacatggt gatatccgcg
     4081 ccgagctgct ccgcgagttt tcgcttatag gcgctgcgtg taatgccgat taccggataa
     4141 gctccccagg atttggccac ctgcacatga atttgcccgc cgactccaag acccatcaca
     4201 acaactgact gccccggaaa tatcttcatg cgactgtggc cgtgcacgca ggtagttgca
     4261 acctgaatga gcggcgcaac attgctttcg atcgaagcag gcaatggata cacatgtgtg
     4321 cgtggagcaa cgagatagtc cgcaaatccg ccgttggagt ctcgaccgac gagggaaccg
     4381 tttgggcaaa gattgtactg gccctcgcgg cagttgaggc acatgccgca gtacatcgct
     4441 ggatcgacga tgactcgggt gccaattggg agcgaggcgt ctcccccgtc gacgacttcg
     4501 ccaatcattt cgtgacccat gatcagcggg tggcgaacgg ggattgcccc ttcgtaaatg
     4561 tgcaagtcgg tgccacagac gccgctatac gtgactcgga ccagaacctc gttgtgtccg
     4621 atggcggggc gcgcaacatc agacaaggcc agttttcgcg ggccttcgag gacaattgct
     4681 ttcatgaggg gttttggggt caatccgcgt tgtcctattt ggtgattctg gtgatgtcct
     4741 gatcgttaga ttggtcgtgc gactggatgt aaggaggtcg gggctttagg cccgacattc
     4801 catgtcgcat ggtcgagggc tttagcccct gaagcttcag aggctaaagc ccttcgaatc
     4861 gagcgctaaa tgtccgacct gaaggtcgga cctccgcaaa acaaactcaa tcggcggctt
     4921 ttccggagcc tacgcggcct ggaagagtcg cgttcaccac atcgcggaat tcagcgtccg
     4981 tcagcaaacg cttgcgctga agcgagaaat ccttaatggc ggcaacgcac ttcattgctt
     5041 cttcttcgtt gaattgcacg ccgagctttc tcaatcccca cttcacggaa tcgatgccgc
     5101 tgcccttgcc gaatacgacg ctgaccggat cctgcccgac gacgtcccaa cggaacggga
     5161 aaagttcagt ggcgttttcg acgccgcagt tttcaaacca gctggcgatg atgccagact
     5221 cgatcttgaa gagcgagtcg cctacaacct gccggttgga tggaacagcg tggccggaaa
     5281 gttgcgccac gagcttcgac aggccgtaga acttgtcata cttgagacct gtgtcgatgc
     5341 catagagagt gagtagcgcc atcgctgtgt cttccatcgg cacgtttcca gcgcgctcgc
     5401 caagtccgag cacggtggag tggacgactt cgacgccttc cgcgagcgcc atgatggtgt
     5461 tggcgacgcc catgccgaaa tccatgtgga agtgggcttc gaggcgcttg ttgatgcgcg
     5521 ccttcacgga gcgcacgagg tactgaatcg aatgcggcga gagcacgccg aatgtatcga
     5581 cgagcgccaa cgcatccatg tggccttcgt tcgcgacgcg caggatcagg tcgagcaccc
     5641 aattcatttc agcgcgtgag aagtcgattg ggaagaacac gacttcgagg ccctgctcgt
     5701 gcgcgtattt cgtcgattcg atagacagat cgatcgcctt ttccatcgac cacttgtaag
     5761 cgtacttaat gatgtgctcg ctggacggaa cttccatcac aacgcccttc acgccgcaat
     5821 cgaccgcgcg cttgacgtca tccaccatgc agcgcgaaaa cgcgaaaatt ttcgggccga
     5881 ggttgcgctt cacgatttcc ttgatggcag ccgtgtcgct gggggaaaca acgggcatac
     5941 ccgcttcaat gcggtgcacg ccggctgcgg ccagtccttc ggcgatgcgg attttgtcat
     6001 cctttgtgaa gatcacgccg gtttgctgct cgccgtcgcg cagcgtcacg tcgtgaaact
     6061 cgatcttttt cgcgaactta aaatcgccgt tcactgctgg attgaaattc cagggactta
     6121 cgaaccactt gtctgtcttc cacggtgtgc ttgacatgat gactatctcc ttatgaattt
     6181 attttacttg tgcgcgtttg ccaatgcttc gggattgact ggattgcgtg gagcctgccc
     6241 ggagaggaca cgcacgacct cttccgcggc tttggtctgt aattcttcga gcgattccac
     6301 cgaatagaaa ctagtgtgcg gcgtcaaaat cacattgtca cggccaaaca ggggtgagct
     6361 ggtaggcggt tcctgttcaa gaacatcaag ggccgcgccg ccaatttgct tggcgtcgag
     6421 agccgctgcg agcgcggctt cgtccacgac cggaccgcgt gacgtattta cgatcaacgc
     6481 gccgggcttc atctggcgga acacatcggt gctgaacagg tgatgcgttt cgggcaaaag
     6541 tggcgagtga atggaaatgt aatccgaaac tttcaggagt tccgcgaatt ccactttgcc
     6601 aacgcccgcc gcatccatca cggattgagg aacgaatgga tcggaagcca caacgcggat
     6661 gccgaaggct ttcgctttcg gcgcgacgag ctgcggaatt cttccgaatc cgacgagacc
     6721 caaagtcgtt ccgcgcaggc gatgaattgg cacaacggct ggcatttccc agcgaccggc
     6781 atgcgcgcgc gcgctggaag cgggtatctt gcgaatgagg gaaagaagca gcgccacggc
     6841 gtgatcggaa acttcgtcga tgcaataatc cggaactttg gttacgacga ttccctgcgc
     6901 cgttgccgcg gagatgtcca cattgtccac gccgattccg aaacgcgaga tgatcctgca
     6961 cttcttcatc tggccgatca tatcggcatt aatttttgcg tacgtgacga gcaccgcgtc
     7021 cgcatctttt gccaccgcca caatttcttc gggcgtgggc ttagctgcca gacgcaactc
     7081 ggccccagcc cgcgcaacca ccgcgcgggc caggtccagg ttaggaaata cagagtctgc
     7141 aactgctacg agtagagagg ccattagcgc ttcatggcct cttccatgtg ttccatcagg
     7201 gtgaagacca caggaccctt ggcactcacc aaaacatttt cttcgaggcg gcaggtctgc
     7261 tgtaatcccg gatgtcccgc aaatgtttcg atcgcgaagt acatgttttc tttgatctcc
     7321 gcgggatact tgagcgaata cgcgcgcgac atccacatgc cttcgtacaa cgtgatgccg
     7381 atgctgtgcg cgaactgttg cagcgtcaca gtgccgaact tgtcatcgtc atattccggg
     7441 aaggcattgg caacgtccgc gctggtcatg ccggggcgca gcttttcgat tccggcgtac
     7501 atggagtcgt acacttcgcg gtacagatcg atttccttct gggtcatctt catgccctgc
     7561 gctccgccgc acttgaagca gcgaacgaag tcgatgaagt agccggaagg accgacggcg
     7621 ttaatgtcaa cgataacgag gtcgccctgg cggatcagct tatccgtggc ccagcggcga
     7681 tacgggctgg tgttgccgcc cgacgcgaca atgatgtcat aaataatttc gcagccgcgt
     7741 tcgagcatga attcgtgaac cttggcttcg atttcgcgtt cacgcacgcc cggcttcagc
     7801 cattcgtact tgatcttcca catcgcggca tcgccgatgc tggaggcttg cttgagcaac
     7861 tcgatttcgt cctgcgtctt cacaacgcgg gcctgcgaga cagccggcca gccgttcaca
     7921 atgttcaggc cggtcttctg gaacgcttcg agagcgggca tatcaaagtt atcgacgcca
     7981 atgcgctctt tggtcacgcc agcctctttc aggacatcca tgacgctttc cgccatgcgg
     8041 ccagccatgt atgggacggc accctcggcc cactgccagg tgatggcggg gcgaatcttg
     8101 ccctccatcc agggcaggtc aatctcggcg cagtgcaagt ccgatccggc tgtttcgaac
     8161 aatacggggt cggtggtatg ggtgacaacg caatagcgaa tattaatgtt gtatttccaa
     8221 ttgccttgat aggaggccgt ggcgtagcgg atattcgcgc cggcgaaaag gacgagggcg
     8281 cctaaatcgt tcgctttcat ggaagcgcgc aatttcgcga ggcgctcctt gcgaaggcgg
     8341 tcaaagtcca cgcgcatttg ccaatcggcg ccggtctggc tatatacgcg ccggggatcc
     8401 caggaatgag gctgaccaat gaatcgtgtg tccataattg tggaagaaac atcctttcat
     8461 ctgttttgga tatgaggcag ctcacaaata taaaacaatt tgaaggattt tgctcaaaaa
     8521 atgttgttaa gtcaaagttt aataagcttg cgcattttcg tgtgtcgtga gcgaaatcgc
     8581 aaatcaacag cgggcacgtt gaagattatc ctgcggatac cccctcgttt cattgtgttc
     8641 actctgcatt taaaacgtcg aacattcggc ccatactctg tgtgacaatt ctgaaccgac
     8701 atgcggcatt ccacattagt tgcaatgggt gtccggtggc tcagcaggca gtgctctgtt
     8761 gttctatatg aatttgcgac gcccgccgat gagaacccgg acgtgattgg atggatttcc
     8821 ggcgcgggtt ccgtgctcat tgaatgcaaa ttaacccgct cggatttttt aagggatgca
     8881 gcgaagcccg tgcggaaaaa tccgcgcgcg gggatgggac aacgccgcta ttatttgtgc
     8941 cctcccgagc tgatccaggt gaaggatctt ccgccgaagt ggggattgct gtgggccaac
     9001 aagggccaaa tcatcgtgaa gcgcgaggcg cgcggacact ccgaaagaaa tcttatcgcg
     9061 gaggttcagt ttctcaactc aatgctcagg cgcgcgcaaa tccgcatcgg cacgcggccg
     9121 ctctccgaat ggctaagagg cgagaacaga tttgaggcga aacgcggaat atcgccgacg
     9181 cgggagccca tcgttcgcaa agcaaaatca tcggtttcag gatgagtaca acggccgttt
     9241 atctgtcgcg aaaaagaaaa atcacatcga gcacggcgag gatcgcaacg agaagttcga
     9301 gcacgaacga gcgagtttcg ttaaatcgat cgaccatcga gtcgtaaagt tcgccgaacg
     9361 tgtccaactt ttgatccact aaattccggt aatcttccac gcccattcgc gtggccgcca
     9421 aacggtagac ccgggcatag taaatatcac tgacaaattt gatcgcgtta tcgatgcgtt
     9481 cggtcaattc cattacgtcc agccgaatcg tgttgaagtg ttgagattcc cgaggcagcg
     9541 tcaggcgaga ggttaaaata tttcttttcc gttccagcgc cagataaaaa tccgcgagca
     9601 cgcgcgtaag gaagcggtcg taataacgaa actcaagcag ctgcatcttc gcgtattcga
     9661 gaatttgagt aaccgcggcg gcatcttccg gaccgtcgac aactaccgcg ccatgcgagc
     9721 cgaccagggc taaatccgaa gggtaatacg acaaactcga ttgcaaagcc tcgtcgatgg
     9781 aatggcgcgc cagcatggtt gtctcgccgc gaatcagttg caccagttcc ccgccgtaag
     9841 aggtaatgag ttcgtcggcc gccggttgca ttcccctttc gtcgagaatt ttgtgaattt
     9901 caacgacaag ataattttcc tgaagccagt cctccgtggc gcgggttacg gcgggccgaa
     9961 tctgctccag ccggcgcagg acaagttctc tgacgagagg ttcaacgtcg gctgcatcca
    10021 tccaccggga actttgttct acaagagtag tcatatcgca gtcgaatggc acctcgacct
    10081 gaatcacgat tgcgccaaat ccgtaatact tcaccgggca agcaacattt cccccggcag
    10141 ctacggaaat acaatcagcc atttcgagca cgggaggatc ctcgaaacgt acatacccag
    10201 gcgtgcgccg gggaaatgcc tgcccggatg aaccgactcg cgcgcccagc agattgcgca
    10261 gcttgctcag atcgatggat tcggccacgt cgtagagaaa caggagccgg aataagcctc
    10321 gtagctgcat acctgttttt accatcaatt gaagcaccgg gagaggatga ttctttagcg
    10381 ccagaggctg gtagtttgca gaaagggtga tgattcaaaa aaacagcggg attagttcct
    10441 tgcagattac gaggcagctt ggggctgttc ttcgggtaat atctcagctt gaaattttag
    10501 ggtacgcatt tcaggccacg cggtccgtga tacggacatg cgcatcggtg agatgaagcc
    10561 cgtacttttc gatgtcgtcc aggcattcgc tggtattcac acccggtgtt gattttccat
    10621 cccggttcag atccaaagat ttgaacgcag tccatgggcc gaattgcgaa ggcaagttgg
    10681 ttccgtttga atcttctgtg aagccaaaca ctcgacctgc acctctaaaa atatggacta
    10741 gcattagcca tgctccctga ttcccggtta gtttagcgca acaggcccga gcagcgctat
    10801 ggtttatcaa taaccccaga attcaaactg agacattacc gttcttcgta ggtgctttac
    10861 tgaaagccga atgtggccta aactgtgtgt acttcgtccg tggtgagcgt agctcagctg
    10921 gtagagcatc ggtctgtggc accgagggtc gcgggttcaa accccgtcgc tcaccccaaa
    10981 attttcaaac atcagtctgg aatcagtcca agttcgtcga ggaactccat tacatgcaca
    11041 tgaatcccat cagaagaaac ctcgtcgctc gccccagcga ctggccctgg agtgattttt
    11101 tattttatgc gaagaaggaa tgcgcgctga ttcgcatcga tccaataagt tgagccagtg
    11161 ttgcctaaac gtcaaaaaac ctgcccttga agtgcgtcaa cggcggggcg ccccaaattc
    11221 aaatataaag cccgggccac ccggccggtt gatccgtcac acttctgagg atgggcaaca
    11281 aatgctggcc aatatctttt ttagaaacgt atccggcggc accggcttcg cgagcctgaa
    11341 gaccgacaac ggccggatcg ttttggctaa cgatgatgac tttcgtgttg agattttcct
    11401 tctgaataag tttggtggct tcgagtccgt tcatgcgagg catcgaaatg tccatcagaa
    11461 taacgtcggg ccgcagagat ttcgccttat cgactgcttc aagaccatca accgcttcgc
    11521 cacaaatttg caattcggga taggaaacaa ggaaagagcg aatcgctcgc cgggtgatgt
    11581 catgatcatc cacaatcagc acacgaagag cgttcactct tgctcacctc gaaaagtgaa
    11641 aaatttcgcc tgcaaataat tagcgcccga ctttgaatcc gcataattcg gctggagaat
    11701 gagcaaagtt ttttgatcct cggatcgccg ggtaccgata ttaaaataat taaagaagta
    11761 tgcgacctat cgtcgtttca atttggcatc aaccaaaaac aaaacctgaa tactaaatag
    11821 gattgacggg ccacgtacat acggggtttg ctgtaggagt gcatttataa attcggaacg
    11881 ggacccagac atttggaact cgagtttggt ttttcgtgaa attcaggact tggaaattag
    11941 aaacgtgatt taatgggtca ccggaatgat ccgaaataca gtgatgactt attgcgcgag
    12001 gtaatgcccg tgaagcattg caatgcgttt ggcaacggaa gcggacttga ggttcgttcg
    12061 gatgaaccgc ggctttcgcg gcgaaaagtc atgggcatga tatccgccgt gggagcaggg
    12121 atggcgctgg cccctcgttc attcgcgcag agcgttgcgg ctgggccacg cattattgac
    12181 acgcaccatc acatttttcc tcccgcattt acggccagga atttgaaacg catgacggat
    12241 gattccccta cgtttccagg ttccttttat acgaattgga cgccgcgtta ttcgctcgag
    12301 cagatggatc aaaatggcgt cgctactgcc atcggctcga tgaccagccc gggcgtttgg
    12361 tttggaaaca atgaggaagg gcgcagaaat gcccgcgatt gcaacgaata cggcgccaaa
    12421 ctcgttcaag atttcccggg gcgtttcgga atgtggggtg cgattccgct gcccgatatc
    12481 gaaggaagcc ttcgtgagat tgaatatatt tacgatcacc tgaacctcga cggaattggc
    12541 ctcatgacaa gctacgacga cggaaaattg ctggggcatc cgaacttcgc tccggtgctg
    12601 gaggaactca atcaccgcaa ggccgtgatc ttcgtacacc cgacgatctc ctgctgtgcc
    12661 atgccgattc gtcacttaaa ccgagtgcag atcgattttc ccacggacac gacgcgcaca
    12721 attacggatt tgattttcag cggctcgctg atccgctacc ctgatattcg ctggattttt
    12781 tctcacggcg gaggaacggt tctgatgctg acgccgcgcc tggcgggcgg caatctgacg
    12841 ccggaagagc gcgctgtaac gattccaaat ggtttcgagc acgaattgca aaagttgtat
    12901 tacgacattg ccagcgtcgc gctaagcccc atacccatgg ccgcggtgct caagggtatt
    12961 cccaaagagc atcttttgtt cggctcggac attccatttt ttacgatcga acggatcgct
    13021 acggctgtaa ataaatttga ggtgccgtcc gccgatctcc gtctcctaca gcgtgagaac
    13081 gctctgcagt tacttccgcg cctgaaggct taggcttcaa agtctgtgtg agaactgcaa
    13141 caccagaacc tcagcggcta aacaggctgc ggtaaaactc aacattttgt cattccgagc
    13201 gaagcgagga atctctcctt ctttaatttt cgtatattaa aatcgaagag agattcctcg
    13261 cttcgctcgg aatgacagaa taaactactt tttccgcggc ctgtaaagac ggattcattt
    13321 tgcaggattt atgtcgtggc tgaagccacg acccacaacg attctgcagt tcacacaggc
    13381 tcttcagcct gtcttacgaa cacatgcgac gttaccgcga ttgctcgtaa acccgcgcca
    13441 cttctccagc gacgttattc ggattcggcc aatctggatt cttcgctttc aactcggcgg
    13501 taattgtctt tccggcatcc tcaaccgaaa ccccctgctt cttcagttcc agcgcccggg
    13561 actgtagagc aagtagatac gcacgctgcg ttgcgatctg cgacgcatcg acgatggctg
    13621 gaccatgatc gggaacgatg aatttcggat gcagggcttc cacgttatcc agaatcgaga
    13681 gccagccctt cacgctcgcg tcgggattcg gcatgatcgg gaaaatatct ttctgaacga
    13741 tatccccagg aatcagaacg ctgtcctctt cgacaaatat caattcatct cccttggtgt
    13801 gcgcggggcc aagccaaaac aggcgcgcgg tcacgccgcc cagatcgact ttcgcttcgc
    13861 gatcgaaaac aacatccggc acgcgaaatt tcacatcttg cagcagttca agattctgtg
    13921 cggacatctt cttgaacact tcgatgtggg cggcaactgc cttgttcatt tcgtcctgct
    13981 gcgcgctgtt ccgaataatg atcgtgcttg cgggaaaagc ctgctcaccc gttgtgtgtt
    14041 cggagtggta gtgcgttgtg gttagataca ggaccgggcc tttggccagc ttttgttccg
    14101 cgcgcatgac tgtcgcgcca ttcttctggc ctaagccggt atcaaccacc atcgttgctc
    14161 ggctaccgac gataatcgca acgttgggaa aaccttcgat cacgtacacg tgatcggaaa
    14221 cgcgcttcag attgtcttcg gagagcagtg gctgcttgag ttgtgcccgc gagagggagg
    14281 gcgtcacaag cgctaccggt aagataagga aaaaagtaat tcgcgcggct gaccacttat
    14341 ttacaatcga catcgaaggt ctccctttta acgagtgccg gcgagacgcc ggcgctacga
    14401 aaaacttttg aaccgagacg cgtttcagat cacgccggaa gatgcggaac gaccagttgg
    14461 aatagtttca gtggggctgt gccaacgtgt ttgatcgtca cggaatcgga tggacctaca
    14521 taaacaccgg cgcccttgct cacaccgtgg tccttggaat ccatcgtgat ggtgccttcg
    14581 ccgtccatca agtagaccaa ttggtgcgta tctttgtcgg aacggacgtc gagtttttca
    14641 ccggcattca gccagcgcaa cgaacccact acattttttg cgccgcagag ctggttgttc
    14701 agaatttccg cgaattcacc cgcacctgaa gccttcatgt gcggaatctt atttgtatcg
    14761 ataaaggtca gtgacatttg agcaatcctt tcaattccga aatattagat tccgcgccaa
    14821 ccaatttgat ttgtgcggct tgcaatctca ctgcctgcgc gactaactct gcatccagat
    14881 tccgccatcc acgcggtccc acttcaaatc atccggcgtt ccttcaagaa tcgtggtctg
    14941 gaagcggttg ggcgcctgga attcaaaata aaaaagcggt tctaccgcat gcagacgatg
    15001 cttttcttcc gcaggaataa aaaccatcat gccgggaccg atctcgcgag ttccttcctc
    15061 ggactgcacg gtggcctttc catccatgat gaaatagaaa tgttcgcaat ttttgtgcat
    15121 gtgatatgga gatgcggcgc cttgtttgta atacatgatg cccgccagca tgtcatcaat
    15181 cttagtcagc tctttattga cgaagaaaac ccgctcgcgg ccattcgata acagcctcgg
    15241 caattgatct tgattgaaaa tgtatgccat gattcggcct cccattgtga tcggcagtcg
    15301 cccgacctga cgttgcgcag caaaatttgt aattacctga acgtgcctta ctcgctatgc
    15361 ggcgattgaa tgtgcacatg ctagcagttt tttcgcggca cttccagaat tggagatatg
    15421 ggggagtgaa ccggcgaaaa gcaaacgaaa aagaaaagct gcccgctcga aaaatggccg
    15481 gccgctacaa agcaaggaca gcaacatgat tgggtttttg tataacctca ccgttccgtt
    15541 cacggagaac gttctcggtg aaccgtgatc ttcggaacga tgaggtcgct cttttttgct
    15601 cagggcttct gacccggaca acttttactt ttttggaaga ccgaaaatag tcaccgtcgg
    15661 atttccgccc ttaatttcag ggaagctctt gttgccgatc acgttgccat cgcctgaagt
    15721 gacggcgaca tattgaatgc catgggctga gtaggtaatc acgccacctt gaatggaatc
    15781 tcccaagtca tagtgcaaga ggacttttcc cgtttgcgcg tcaaccgcgt caaaaaaacc
    15841 tccgctttcg ccagtaaaaa taaggtctga agcggtgatc gcaactcccg caatcagcgg
    15901 cgtgggcgat tcataccgcc aacgctcttt tccggtagcc gcgtcgaagc cagtcaaacg
    15961 gccgcgcgat ccgctgaaag ctccgtggtc aattggcccg ccgaagtagc tcttctctcc
    16021 ggtgttggct ttcgccacgc ttggcgcctc tttatccttc atgttcatcg agcaccactg
    16081 atcattcgat ggaatgatga gcaggctaag cttgggactg taacctccgc tgttccactc
    16141 gttgcctccc aatattccgg ggcagacttt aatcggggta taagtcaacg gcacttcatt
    16201 gttcagctgc gtcgtaaacg gtgagctgta gacgagcttg tgcgtttcgc catccaccac
    16261 gcgcaaaacg ccgtccttgc cagtggatgc gatcatcgtt tgagaattta ctttgaaaat
    16321 cggattgacg tgcgtgatgt cgtagtcatc cacgtcgtgc ggaataaatt gattgtacca
    16381 aagcaggcgg cctgtcttcc catccagagc gatcatggaa ttggtgtaca ggttgatgcc
    16441 gggtctcgca tcatcgtaaa aatcgggagc cggattccct cccgcaatat agaggacatc
    16501 tttttcggtg tcgtatgact gcgcagtcca gagggcgccg cccgcgtgct ttcgcgcttc
    16561 tggatctggc ccccaggtgt cagcgcccgg ctcgccatcc tgagggatga tattgaattt
    16621 ccaaacctgt tcgccgtcgc tcagtttgaa tgcgccaatc catcccgtgc ccgctctttc
    16681 cgatccggcc ggaccgatat aaatcaaatc gccgtgcaca agcggcggca tggcaatgaa
    16741 atggccttcg tcaggattgg caatttgctt ggcccacaga agcttcccgg tttccgcgtc
    16801 cagcgaaatc agaaaatcat cgcccgtgcc gcgaataatt tttccattgg caatggccgc
    16861 tccgcgatgc gggtgaacgc gatcggtcgc gcgcggcacc cactgatagg accacaacac
    16921 gcggcagtca gctgcatcaa gcgcaacagt atagtgatcg ctcgtcgcat aaatgacgcc
    16981 tgcggaaaca atgggctcgg attcggaggg aactttttcc ggaaaggtgt aggaacatat
    17041 ttttgcgagc tgcttcacat tacccgagtt gatctgcttc agcgggctat atcgcgtccc
    17101 atttaaatcg tgatccgcgt aggtccagtc ggcatcgtca cccgcggcgg ggctcgcagc
    17161 aagcgatttg ccgccaaatt tctgaatggt tccaaacgaa atcgccagtg tgcccaggca
    17221 aagaaaaatc cctgcgatcg ttcgcaaagc cgaaactcgg actcgcaatc tggtcatgcg
    17281 catattattt ttcctctgtc tgaaatcaga agtgcggtca aatctgctag cgcaactgtg
    17341 tttccattac ctcagtttgt caagcaccgc tgcaactgaa acgcagctct ttgtggcgcc
    17401 ggcgggacgc cggcgggttt gcggcctaca cgatgaaaat caaaacccac cgctgggacg
    17461 ccagcgccac aactacaaca ccaatttgaa atttggaatt cggcgcatat aatgcgtcag
    17521 gacttctcaa atacaacaaa catctaatca taaattgcga atttgaaagg gtgaaattca
    17581 tggctgagcc taaggaccca acaaatctgg actcaaaaac tctggtggac cggcgtgatt
    17641 ttctaggagg tgcaactgtg ttagcaggat catcgcttct tggactggcc tcggcagggc
    17701 gcgcggacgc cgctgaaggg gtcgcggacc gcatgccccc aattcccgct gaaaaatgga
    17761 cggaagcgca aaagaaggcc gctgaagaaa ttacttccgg cccacgtaaa gacctggtcg
    17821 gtccgtttat tcccctgctg cgaagcccgg aatatatgag ccgcctgcaa agagtgggcg
    17881 aataccttcg ctataagacg aacctcgggt cgggcatcag cgaattcatt attcttctga
    17941 tcgctcgccg ttggacgcaa caatttgaat ggtactcgca cgaagcgctg gccgcgaagg
    18001 cgggtattaa agctgaaacg atgaaggcca ttgccgaagg gcatcggcct acgaccatga
    18061 ctccggacga ggaaatggtt tacgaattcg tggcggagct tctcgcaaat caaagtgtca
    18121 gtgatccgac ttacgcgcgg atattgaatc ggtttggcga acaaggtgtg atcgacataa
    18181 ccggattgtg cggctactac acttcgctcg gtatgctgat gaacgtggcg aggacgcccg
    18241 tgccgcccgg agtgacgcct ccgctggcgc agttcccgcg ctaaagcttc agtggaatag
    18301 gcttcagatt ctgtgtgaga actgcagaat ctttgtgggt cgtggcttta gccacgacat
    18361 aagtcctgca aaatgagtgc ggctttagcc gctgaaactt caattttgca gttctcacac
    18421 agactcttca gcctgttggg tttgattttg ggaatcgttt gaaacccaaa cagttaaagt
    18481 tcagacgaaa tgccacgggc tgaatgaagc ctgtgctaca tatcacctgc gaacaaactc
    18541 atgaggctca tcacgtttcg tcatctgggc caacaggaaa ttggcgccct cgcggacgcg
    18601 gacacgaagg tcatccgctt gcaggccgcc gaagaacttc gttgcggcga agccaatccc
    18661 cattttcaag cgatgctctc attcctccag ggtgggggca catcacgcga cgcagcacaa
    18721 cagacttttg agttcgcact cgcccagcgg cccgacggca tagtgattgc ccgcgaaaat
    18781 attgagcttc tttctcctgt tccccgcccg gaatcgattc gcgaattcat ggtgtttgaa
    18841 cagcacgtga ttaattgcac gcggcgtttc gagatgccgg gctggagagc ggcagtcgat
    18901 cggttcgtag agaaaatgtt tggacgcaaa gcgacacttg cttaccgcaa ggcacagccg
    18961 tggtacgaaa ggcccgtcta ttacaagggc aaccgcatga atgtaatcgg cgatggcgcg
    19021 gaagtcacaa ttccacttta cacgcagaag tttgactggg aactcgaatt cggcattttc
    19081 ctatgcaaga agggacgcga catttccaag ggaaaggccc gcgaatttat aggcggttac
    19141 acaattttta atgatttttc ggcgcgcgat attcaaaaaa gagaaatggg cggccgcctt
    19201 ggccccgcaa aaggaaagga ttttgatgga gggaactcga ttggtccggt gctcgttacg
    19261 cccgatgaaa ttcccgatcc ttataatctg acgatgagag ctcgcatcaa cggcgaagaa
    19321 gtatccttcg gccacaccaa agggatgcgc tggaagtttg aagacttgat tgcatacgtg
    19381 tcacaggacg agacccttta tcctggagaa ttttttggct ccggcacggg aacagttcca
    19441 gaatcgggct cgaatcgctg ctgctgcggc ctcgaaatgg ggcgcttcct gaaagcagga
    19501 gatacaatcg agctcgaagt tgaaaaaatc ggcaagctca cgaaccgcgt agtgggacaa
    19561 tcttcgtaga tcatgctttg cgtaggcgtg cgataagcgc atcagttcac ggaaggaatt
    19621 tccatgaatt ttaaaagcat ttcaatcgcg cttttcctga tttcgatttc catatccgta
    19681 agggcggccg cgcaagcccc cgacccagct ctcatggcgg agatccacaa gattcgcgcc
    19741 atcgacaacc attcgcatcc tcctcgtgtt gttgagaatg gcgaaaccga cgatgaattc
    19801 gacgcgctgc cgtgcgatcc actcgagcca accgaaccaa acacgatgac gcgtccggaa
    19861 aatcctcaat atttggccgc atggaaggcg ctgtggggat atgaatatac cgaccgtagc
    19921 gacgctcatg taaaagagct catcgccaaa aaaaataata ttcgtcagga gcaaggcgat
    19981 cactatccgg cctgggtgct cgatcaattg ggcatcgaca ttgaatttgc aaatcgcgtg
    20041 gcgatgggac gcggcctcga cgcaaaacat tttcgttggg tgccatttga tgacgccttg
    20101 ctgtttccct tgaacaatga tgcgttggca gcggaaactc cggatcgaaa attcttcttt
    20161 ggccgcgaaa atatgcttct ggcgcgctac cggcgcgaac ttggcgtcgc gtccatgccg
    20221 ccttcgctcg ccgaatatac ttctcgcgta attactccgg agcttgagcg ccaaaaaaat
    20281 acgggcgcgg tggccatcaa atttgaggcc gcatatttac gctcgctcga ctttgagccc
    20341 gcatccgagc gcgatgccgc ggctatttac gccaaatatt tgcatggtgg cgttccggta
    20401 aaagaggact atacgcgctt gcaggattat ttgtttcggt acgttgcggc cgaggctggc
    20461 cggcttggat tgccggttca catccacacg ggcgcggggt gcggcggtta ttttttctta
    20521 agcggatcgc atcccctgct gttggaatcc gtgctgaacg atgcgcgcct tcgaaaaacc
    20581 acctttgttc tgctgcatgg cggttcaggc ccgttcacgg gcgaaattgc cccgctgctg
    20641 atgaagccga atgtatacgc ggatttatcc gagcaaacat ggatggaatc gcctcaccgg
    20701 ttggcggaag tcctgcgcga atggatggag tggtatccgg agaaaatttt gttcgggact
    20761 gacttatacc cgggcagcgg tgcgtacgac tgggaagaaa tcggctggca aacgtcgcaa
    20821 acgggccgcc aggcactggg aatggcattg acggaaatgc ttcatagcgg cgaaatcacg
    20881 gtgctgcgag cgaatgaaat tgcgcggatg gtcttgcgtg aaaacgccat aaagctatac
    20941 cacttgcaag acgctcaata gttgaaacaa gactgcaatg gaaaacgctc gttccgctgc
    21001 gaaaagtacg gagcggaacg agcgtctcaa aatcctagca ttcgtccggc aacagatttc
    21061 gtagaggccg cccgaactgt gggcggcctg aatctacttc gcatcacctg cgcgtcaccg
    21121 gtcaaacaaa tatttacatt ccggaagaaa attcaattga cttggtcttt ccggaaacct
    21181 ggatttcagt caatttgtca tggtcaatga tgaaagtggt ctcattaatt ttgacgctgg
    21241 aatctttcaa agtgcagggg acttcggcga caaccttatt tccctgttga aacttcgcct
    21301 tgcttccatc tgcaatgacc ttatattcgc ccgccgcaag ctgcgtcgta ccgactgtgg
    21361 tgggcaccgt gagcttcaag ttcgttgtga tggtatcact cgaatcattt ttggcccagg
    21421 ccgaaacgcc catagtagta agtgccataa ttgccagaac aactggtagc aaacgtcgag
    21481 acataaataa gaaatctcct ttttctatgt tggaattgta ttgttccata tcattactta
    21541 atttaagggc cctggagaac tacagtgcct gtggcaaatg aatccgctaa gtaccctttg
    21601 cacaattaga tgcttttggc ggaaatctgg ttacattttt gcgtgggttt cttatttgct
    21661 taggactctg gccggctcaa tttaactttg tgaatcagaa ataatctatt tccagttgtc
    21721 tagaagcttt ctgactgcaa caacctgccc caagtggtac gcgttatgat ccgcaacgag
    21781 cagcgcttcg cgcagaattg tttgtccggt tccatgagga atcttctcaa aaagatccct
    21841 tttgcgattc gataccaatt tcttcatcgc atcgagatct cgcgcgaaca tttttacgct
    21901 tttgtcccag gcctttgcgt ttggcggttc gggagcggca ggccagtatc ccgtgggcca
    21961 atcgggagaa acgtgcctgg cgtcgcgcga aaattccaga atatcccatt gcgcgatccg
    22021 catatgctca agcaattccc aaagtgagtg cggcaatccg ctggggcgga cgcctcttaa
    22081 ttttggagga atgccgcgaa acgatgcctt ccaatccgcg tgcgccccgc gcccggataa
    22141 aagctccaga aggtgtttgc gcagcttgtc ttcgttcacg aatccccctc gttgcgacga
    22201 attcaattgg ccgattggat tccttgcgcc gaaggaattg acttgcggct tccgcaggca
    22261 tccgctgaaa ttccggtttc ccaatccgcc aataattttt gcccctcgtc attgatttct
    22321 tcgattgttc gcggccttcc ttctcccgaa ccgggaaaca ccagataaag aacacccctc
    22381 catgtgccac cattgcgcgc attggaacga attcccaaat tttccgcgag cgccacggaa
    22441 ccctcaccga tccggtcaaa cggaccgacg tcaccaaaaa ttgcgtaaga actttttcca
    22501 tttcgttcgt tgaacacgat tgcgaaatct cccactcgcg ctcccagctg tcgcgccaac
    22561 cctcccggca aaaccaggaa aggaatcttc gaagcatcga cataccgcgc gggatcactc
    22621 accgctttgg aacggtccgc gagagcggtt tccgaaacgt aatatccagg gaacggatcg
    22681 ttcggcccct gaatgaaagg ttcaccctgc gcatccttcg ccaagccttc ccaaatgccg
    22741 ggagcgccgg cattttcgag gtcgtcgatg cccgaattgt ccggatggta ggcgttcggc
    22801 gcgccatccg cgtcaatcgt cattccggat tcgtaaaaaa atgcagaagt tccgggaagg
    22861 tgcccgatcg gaatatcgcg ttccgtttct ccgtcatgcc tcatttcaaa attcaatagc
    22921 agcatttcag agcagtctcc ttgtgccttt tccggagaat cttgcccctc ggcggagaga
    22981 atgcattgag ctaaggctga atgtttttgt tgcaggagga ttaagtcttg gtcgatatgg
    23041 agggctggca aggcccgcgg cacagtttcc aatgaactta ccaaattatt ttgcggatgg
    23101 gtgagaagtt ggctcaggtc gggacccaac tctcccacgg caactttttt gagttcagcc
    23161 gcgcaattgg aatcggaagg ggcagaattt tctgcagaag gaacctgctg gcttctcgca
    23221 atgatggctg aggatataac gataactgcc gcaaacacga aggaactgaa ccgcatatgc
    23281 ctatgcccct aaatttttct cacgcgatgc gtggacttcc gtgagtacgg ccgtttcgcc
    23341 tgtcttcaga aatacattgc ttcacagtat tgtagacgtt taaaacactc tatgtgttgc
    23401 atggaagtta aatcatcaga aaatcagcgt aatgaagtta attctaagct acgagatatt
    23461 tcaatcggct gatctcctcc agacaatcga gcagtagcaa tagccgtact ttacctgtcc
    23521 cttcgaccag ctttaaggaa atcgttcggc tgttaggctc ctttaagcct tgggcttaca
    23581 tctatatttg cttgttgagc tggcagcggt ggcgcgccgt gagggttcgt cgagggatat
    23641 tggtgtctac tttattttcc caaatcatga atcgctgcca attggggaac gtacttgctc
    23701 tccagttgct atgctggatt ttattgctca taggaatatc attcacggcg ccggatgtgc
    23761 gcgctcaaat ttcccctggt cctctttcga aagcacacca atttctgaac ggtttgaccc
    23821 aatgcacgac ttgccatcaa tttggaacga gcgcaccaac tttcaaaccg cgcgcaagca
    23881 agcgtcgctg tccaagcgca ttttattttt gtcccgcacg aaacaagttt ttaatctttg
    23941 gcacgtcatt caccgcccat ttagttattc gttcgcaatc ctcgccatcc tgcacattgg
    24001 ggaattatat acatgacttc cgtatcaaaa cactcgactt taaatggctt gcttgcaacg
    24061 gactccgaac ctagtacaac atattggcag ggcgatgagt atttagggcc gtgtatatat
    24121 ggaacgtact ctgattctcc atatccagtt atattccgtg accgtctggg ggtacaatcg
    24181 ccccctatga ccgcgattgt gtctgtgtac acgccagagg gttttgtagt gggggccgat
    24241 ggattgcggg tagacgcgaa tggcgtggat gttactgata ccgccgtgaa gattttccct
    24301 ataatccacc ccgatttaat cggcgtccat gcgtgggcgg gaaccacata tttgttttca
    24361 ttaggacgcc ccctgtttag tttttcggaa gaagctgatt ctttgatgaa agaattgtcg
    24421 aatgctcccg tccagtcttt atctgatta
//
LOCUS       DASZMB010000194         3986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4872002, whole genome shotgun sequence.
ACCESSION   DASZMB010000194 DASZMB010000000
VERSION     DASZMB010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4872002"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VG272_08920"
     CDS             complement(<1..327)
                     /locus_tag="VG272_08920"
                     /inference="COORDINATES: protein motif:HMM:NF016356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HWF13842.1"
                     /translation="MNEFPKPKIVISKCLGFEACRYNAEMIRDTFVPRLAEFVEFIPV
                     CPEVEIGLGTPRPTLRLVSSSQGMRIIQPTTGMDFTDDINRFSEKFLGSLKEVDGFVL
                     THRSPSC"
     gene            complement(324..1775)
                     /locus_tag="VG272_08925"
     CDS             complement(324..1775)
                     /locus_tag="VG272_08925"
                     /inference="COORDINATES: protein motif:HMM:NF022517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWF13843.1"
                     /translation="MDRTSNLILLTGATGYVGGHLLSELEKSGHSVRCVTRNPDSLAA
                     RHVATTTVVAADLTDAQSLRSAMAGVKTAYYLVHSMNSNAAFEEEDRKAALAFAAAAR
                     DAGVKKIIYLGGLASGESLSSHLRSRQEVGKILRESGTPTIEFRASIIIGPGSFSFEM
                     IRALVERLPVMIMPQWVKTPTQPIAIEDVIAYLLAGLDLQIKKSRVVEIGGSDQVSYK
                     DLLREYAKQRGMRRLMIPVPVLSPRLSSLWLGLVTPVFARIGRELVDGLRNETVVRDP
                     SALQLFSIHPRGFRESIECALLADDQHFARKRWSNNLSRDAGKKIGERNKMESGSRLI
                     DSRTMHTRCPPEKAFLPVEQLGGAIGWYYGDWLWTLRGVLDRAFGGVGMQRGRRDPAR
                     LLPGDKIDCWRVEAVEQGRLLRLVAEMRVPGRAWLQFEFEPDSSGTQIHQTAIFDPSG
                     LLGRLYWYLLIPIHQLLFQGMLRSIVESAEQNQ"
     gene            complement(1862..3124)
                     /locus_tag="VG272_08930"
     CDS             complement(1862..3124)
                     /locus_tag="VG272_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13844.1"
                     /translation="MRKFVVEGMTANSKAVRATKALYYPYVEAEANEGRGLLAALAAH
                     AAVVVTDEFPCYFLPKMVAAAGAHLRVLLEQVDSNGILPLRASPKEFSTAHSFRRFLQ
                     KNLPEHLMNFPHARPLFAVNLPPAKPLPTKITKRWPEFKLDDSISPSNSASEAAEIVH
                     VKTTLQRGGSSAAQKQLHVFLSHGIDSYNEDRNDPCEDGTSGLSPYLHFGHISAHQIF
                     CSVAKHEDWHPEHLALRSNGSRDGWWGMRSAAEAFLDQAITWRELSFNMCHFRQDYDQ
                     FESLPEWARKTLTERANDPRPRKYSFAEFERAATHDPLWNAAQIQLVSEGKIHNYLRM
                     LWGKKILEWTKSPQEALQIMIELNNKYALDGRDPNSYSGIFWCLGRYDHPWGPVRPIY
                     GTVRYMSSENTARKLNVKEYLFKYLAKH"
     gene            complement(3342..3815)
                     /locus_tag="VG272_08935"
     CDS             complement(3342..3815)
                     /locus_tag="VG272_08935"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HWF13845.1"
                     /translation="MELIICVAACAAAGWLGSMWTIPNLHPWYSNLAKPSWTPPEWLF
                     GPVWSALYLIMAVAAWLVWKRVGMASAQMVLFSLQLLLNALWSGIFFRLRLVGPAFFE
                     ISLLWISILATCISFHRIAPLASWLLVPYLIWVTYASGLNFSIWRLNPPPQSARP"
BASE COUNT          966 a         1077 c         1063 g          880 t
ORIGIN      
        1 gcacgacgga gagcggtgcg tgagtacgaa gccgtcaact tctttcagcg agccgagaaa
       61 tttttcgctg aaacgattta tatcgtccgt gaaatccatg ccggttgtcg gctggatgat
      121 gcgcattccc tgcgagctgg aaacgagcct gagcgtgggg cgcggcgtgc ccaaaccgat
      181 ttcaacttcg ggacaaacag gaatgaattc cacgaattcg gcgagccggg ggacaaatgt
      241 gtcgcggatc atttcagcgt tgtaacggca ggcttcaaat ccgaggcact tgctgattac
      301 gattttcggc ttgggaaact cgttcattga ttttgttccg ccgactctac aatgctgcgc
      361 aacatcccct gaaagagtaa ttgatgaatc ggaatcagaa gataccaata aagccttccc
      421 agtaatcccg atgggtcaaa gatagcggtc tgatgaattt gcgtgccgga gctatccggc
      481 tcgaactcaa attgcagcca cgcccttccg ggcacgcgca tttccgccac aaggcgcaga
      541 agcctgccct gttcgacggc ttccacgcgc cagcaatcaa ttttgtcgcc gggaagtaat
      601 cgcgccggat cgcgccgtcc acgctgcatg ccaactccgc caaacgctcg atccagcacg
      661 ccacgcagcg tccacagcca atctccatag taccaaccaa tggcaccgcc gagttgctcg
      721 acaggcagaa atgctttttc gggagggcag cgcgtgtgca tggtgcgtga gtcgatcaat
      781 cgactgccgg attccatttt atttcgctcg ccgatttttt tcccggcatc acgtgaaaga
      841 ttattggacc accgctttcg tgcaaagtgc tgatcgtccg cgagaagcgc gcactcgata
      901 gattcccgaa atccccgtgg gtggatggag aatagttgca gcgcggaagg atcgcgaaca
      961 acagtttcat ttcgcagccc gtccactaat tcacggccga tgcgtgcaaa cacgggagtc
     1021 acgagtccaa gccacaggct agacagacgt ggcgaaagca ccgggaccgg aatcatcagg
     1081 cgcctcatgc cgcgctgctt cgcatactcg cgcaacaaat ccttgtagga cacctgatcc
     1141 gagccgccga tttcgacgac gcggcttttt ttgatttgca gatccaatcc ggcgagcaga
     1201 tatgcgatga catcttcgat ggcgatgggc tgggtcggcg ttttcaccca ttgcggcatg
     1261 atcattactg gaaggcgctc gaccagcgcc cgaatcattt caaatgaaaa acttcccggg
     1321 ccgatgatga tcgaagctcg aaattcgatg gtgggcgtgc cggactcgcg caaaattttt
     1381 ccgacctcct ggcggctgcg cagatgactg gaaaggctct caccactggc gaggccgccg
     1441 aggtaaataa ttttcttcac gccagcgtcg cgcgcagctg ccgcaaatgc gagcgcggcc
     1501 ttcctgtctt cttcctcgaa tgcggcgttt gagttcatgg aatgaacgag gtaatacgcg
     1561 gttttcacgc cagccatggc ggagcgcaac gattgcgcat cggtaaggtc cgcggcaacc
     1621 acggtggtcg tcgccacatg ccgtgcagcc agcgaatccg ggttgcgcgt gacgcagcga
     1681 acggaatgcc cgcttttttc caactcactc agcaggtggc cacccacgta accggtggct
     1741 cccgtcaaca gaatgagatt gctcgtccga tccattgcgt taacccttct ttcaaccatt
     1801 ggccgacttt gcctgagcgt tccacgaacc gggcttcgag aagcgattcg tggtttgagt
     1861 cctaatgttt tgctaaatat ttgaacaaat attccttcac attcagtttt cgcgccgtat
     1921 tctcggagct catgtaccgc accgtgccgt aaatcgggcg gaccgggccc catggatgat
     1981 cataacgtcc gaggcaccag aaaatgccgc tgtaggaatt aggatcgcgg ccatcgagtg
     2041 cgtacttatt atttaactca atcatgatct ggagagcttc ctgtggcgac ttcgtccact
     2101 ccaggatttt ttttccccac agcatgcgaa ggtaattatg aatttttccc tcgctgacca
     2161 gctgaatctg agccgcattc cacaggggat cgtgcgttgc ggctctctca aattccgcaa
     2221 aggaatattt gcggggacgc ggatcgttcg cgcgttcggt tagcgttttc cttgcccact
     2281 ccggtaaaga ttcaaactga tcgtagtcct ggcggaagtg gcacatgtta aaactgagtt
     2341 cacgccacgt aatggcctga tcgagaaacg cttccgcggc ggaacgcatg ccccaccagc
     2401 catcgcggct gccattggaa cggagcgcga gatgctcagg gtgccagtct tcatgctttg
     2461 cgacggaaca aaatatctgg tgcgcggaaa tgtggccgaa atggaggtag ggagaaagac
     2521 cgctggtgcc gtcttcgcag ggatcgttgc ggtcttcatt gtaagaatcg attccgtggg
     2581 aaagaaatac gtgaagttgt ttttgagcgg cggaacttcc gccgcgctgc aatgtggttt
     2641 ttacatgcac aatttctgcg gcttcgctag ctgagttgct tggcgaaatt gagtcgtcta
     2701 atttaaattc cggccatcgc tttgtgattt tcgtgggcaa aggttttgcg ggtggcaagt
     2761 tcaccgcgaa caatggtctc gcgtggggaa aattcatcag atgctcggga aggtttttct
     2821 gcaggaaacg ccgaaacgaa tgcgccgtgg aaaattcctt aggagatgca cgaagaggaa
     2881 gtatgccgtt ggaatcgacc tgctccaaaa gcacacggag atgagcgcca gcggcggcca
     2941 ccatctttgg cagaaaataa caagggaact cgtcagtcac tacgaccgcg gcatgagcgg
     3001 ccagcgccgc caacaaacct ctgccttcat tcgcttcggc ttcgacatat ggataataga
     3061 gcgccttggt agccctcacg gctttcgaat ttgcagtcat cccttcgacg acaaacttgc
     3121 gcattctctc gtgcgcccac ggaaagtccc gccgaagagc ctcgaaaatg attagaggct
     3181 tgcgaagttt agtgcaccat tcgattgcac ggtccaacgc aaaattccaa tgtgttctgc
     3241 ggttggcaat catccaatat aaaacgtact cgccatcggt acggataggt tgggagttgc
     3301 atgtctggat tcgaattgcg ggcacagtat tcatcagatt attaaggccg ggcggattgc
     3361 ggtggcgggt tcagtctcca aatcgaaaaa ttgagtcccg aagcataagt cacccaaatc
     3421 aaatatggca caagaagcca gcttgcgaga ggcgcgatcc gatgaaagct aatgcaggtg
     3481 gccagaatag aaatccacaa caaagaaatc tcaaaaaatg cagggccaac caagcgcagc
     3541 ctaaaaaata tgccggacca aagagcgttc aacaaaagct gtaacgaaaa cagaaccatt
     3601 tgcgcggacg ccattcctac gcgcttccat acgagccacg cagccaccgc cataatcaaa
     3661 tagagcgctg accaaacggg cccgaatagc cactctggag gggtccatga cggttttgcg
     3721 aggttggaat accacgggtg caaatttgga atagtccaca tcgagccgag ccatccagcg
     3781 gccgcgcatg cagcgacgca aattatgagt tccatttaga cagtctccat cctcaaaaca
     3841 atacaggcat cacagtgagt tttgaaatcg gcaggaacga gaaccctatt ctcaattgaa
     3901 cagagcctcg tcccatgacg tgaaaactac cccggcactc agtgcgagtg ctgcacagcg
     3961 cgctcactgt gccatctgcg ctgaat
//
LOCUS       DASZMB010000195        21536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2948897, whole genome shotgun sequence.
ACCESSION   DASZMB010000195 DASZMB010000000
VERSION     DASZMB010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 21536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21536
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2948897"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            668..1240
                     /gene="rfaE2"
                     /locus_tag="VG272_08940"
     CDS             668..1240
                     /gene="rfaE2"
                     /locus_tag="VG272_08940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HWF13846.1"
                     /translation="MKKSFSGLMFVKKCALAFPMGKIVSQEELIQVTGRERRNGRTVV
                     FTNGCFDLIHPGHVRCLAEARALGDLLVVAVNSDRSVRGNKGPERPLVAEQDRAEVLA
                     GLASVDYVTIFDEPTPRELIARVLPSILVKGADWALDQVAGREEVEAAGGRVISIPLV
                     PGYSTSNLVQRIRDLGGAATVSSHDPHSKS"
     gene            1237..4644
                     /gene="mfd"
                     /locus_tag="VG272_08945"
     CDS             1237..4644
                     /gene="mfd"
                     /locus_tag="VG272_08945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HWF13847.1"
                     /translation="MILSSVSETLASIGRQSALELTISELRQGTALERLAGLTQTGKA
                     LIAAIIATELRRPMVVLVESGARAEEFAASMQFFYKALAGQEATEVTILPALDVLPWQ
                     EAAAHAEILETRAVTLWRYAKGEARVVVAPIAAARMRLADATYYAEQARTLAREDPLS
                     LDDLVGHLRRVGYESHDMVEMPGQFTVRGGIVDVFPAEAERPVRLELFDDTVESLREF
                     DPNTQRSVRPIDRVTLPPLVEQSGNGFGGRYGNTEDAPKISHTHSLLDLHEDTVVLLD
                     EPGAIEEASRAFLQGAMEDLEAKKSESLDLLSLHYFTDNWDTLLARHARLELDQLALT
                     REDSETRIIQTQPTSNFHGNVPAFMEEARKRVAAGERVLLAAESLGELERYVDLCHEY
                     EVPFRLGDLEENTTSARLAEDSSAGSVPAVVLCRAPFTDGVIFLDARVAIFGTGDVFE
                     SMRAGTAKRRPKTASFFSDFSELKPGDYVVHVDHGIGQFEGLHQIENGGARGEFMRLR
                     YADDARLYVPLERLDLVQSYRAVEGATPVLDKLGGSTWTARKARVKKSVEDMAEKLLK
                     IYAGRKTGEGIAFSADGEFQREFEDAFEFEETPDQAIAIADVKRDMERQQPMDRLLCG
                     DVGYGKTEVAMRAAFKAATDSKQVAVLAPTTILAFQHYETFRKRFAAFPIKVDLLSRF
                     RTAREQKDVLSLVETGQVDIVIGTHRLLSKDVKFHDLGLLVVDEEQRFGVAHKERLKE
                     LKLNVDVLTLSATPIPRTLNMSLIGLRDMSVIETPPKDRMAIQTVVAPFSELLIRRAI
                     DEEMLRHGQVFLVHNRVETIYAIADLVKKLVPKARVVVGHGQMNEKELESVMFKFVRG
                     EADVLVSTTIVENGLDIPAANTILINRADRFGLSELYQLRGRVGRSNQRAYAYLLVPE
                     DVVLTQLATRRLAALKEFSALGAGFRVAALDLELRGAGNLLGGQQHGHINAIGFDMYV
                     QMLERAVSTLKGEEVKPEMRATINLGLDIRIPEEYIPNENLRLRTYKRIAGITNEKER
                     EEVNSELTDRFGAPHSSVGNLLDYALLKGLAESLLISSIERRANEVAIKFHPETPLSP
                     ENLVKFIRSQKEGLRLDPSGTLWMRLDRRGRVAESVRNVLLPLEMKR"
     gene            4666..5778
                     /locus_tag="VG272_08950"
     CDS             4666..5778
                     /locus_tag="VG272_08950"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWF13848.1"
                     /translation="MNLSKCLIFLLVFASLGIPAGAQTKSTVVEEIVARVNNDIITRE
                     DLDHAHASLDSEAADACQKCTPDEVKAMIAGKEKDLLRDLIDQSLLTQRAKDQGINVD
                     TEVIKRLDSIRQQYKLPDLDALEKEVTKSGQDYEDFKSQLRNQLLTQELMRKEVGSKI
                     IVSHEDIVKYYNDHKAEFVRPETVVLREIFVSTEGKPEADLPALKKKAEGLRDRVLNN
                     GDDFGELAKHYSDSPTAQQSGDLGTFQRSQLDSKIAEKVFALKRDQMTDVMETKTGYE
                     VLQVRERYDAGEQPLDKVEPEISNKLYEQSMEPAMRTYLTTLREDSFLQIKPGYTDTA
                     AVKAEPIEEVAATPDKDDKNKGSRKWGVLPKKKSGT"
     gene            5775..6812
                     /locus_tag="VG272_08955"
     CDS             5775..6812
                     /locus_tag="VG272_08955"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HWF13849.1"
                     /translation="MKQCFVSTLQDGQTVTSHFLVCVKEIRSTREGKTYLRLELGDST
                     GRVEGRMWTGFDAMAATFERDDFVKVQARVENYRNKLQLAIEKIRRAEENEVDPADFF
                     PHTRENVEEMYAKLLEIVASVGNPWLRQLLDSVVQDPEIVPRLKRAPAAKIMHHAFYG
                     GLLEHVLSLCNLCRVVLSHYPEANADLLLSGAVLHDVGKLKELSYDRSIGYTDEGQLL
                     GHILIEYEHLTKKIDAIEGFPSELKTLVLHMLISHHGQYEFGSPKLPMFREALLLHYL
                     DDLDSKMAAIRSALDSDEGEGNWTGFNGALARRILRADKFLGVPAAVIPDASAVAESV
                     ASSADSADGKK"
     gene            6799..7272
                     /locus_tag="VG272_08960"
     CDS             6799..7272
                     /locus_tag="VG272_08960"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HWF13850.1"
                     /translation="MEKSSGMEHIDQPRAAGPRHSILEIAPLELECLSVLWPLGEGTV
                     RDIHRALAASRPRAYTTVMTIMDRLEQKGIVARRKVGRAFLYQPKLSAEEARLKAVEK
                     IVEGFFNGSPEALVAHLSFRGNVAAPPPVPATARVDVSSAPVAELPSRNLDERLL"
     gene            7277..8254
                     /gene="dusB"
                     /locus_tag="VG272_08965"
     CDS             7277..8254
                     /gene="dusB"
                     /locus_tag="VG272_08965"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HWF13851.1"
                     /translation="MLPASFSIRDKNIAPGTVLAPMAGVTDTVFRRMIRTLGGCGLIM
                     TEFTSAECITRNNARTARYLFFAPDEHPITGQLFGAEPEVMARAAGMVEQLGFDIVDV
                     NLGCPARKVVKCGGSGLLRDLKALEQVLSAVRNAVKIPMTIKIRAGWDENSIVAVEVA
                     RLAEQIGVEGIAVHPRTRAQGYTGRADWKVIRDVKQAVRMPVTGNGDINSPEDAMRML
                     RETGCDAVMIGRAASNNPWIFKQISQFAATGSYNQPTEWDRYQLLSMYFRNLVDTEWP
                     DAIGRMKQFTSYFTHGVRNGGELRKNVHTARTTSEILDRVDSFFAQTAI"
     gene            complement(8344..9201)
                     /gene="rsmI"
                     /locus_tag="VG272_08970"
     CDS             complement(8344..9201)
                     /gene="rsmI"
                     /locus_tag="VG272_08970"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HWF13852.1"
                     /translation="MTDAQDNSQTGCLYLVATPIGNLEDITLRAIRVLKEADLIACED
                     TRQTQKLLQHYSIHKEMVSYHAHNELTRAPELVIQLEQGAQVALVSDAGTPVVSDPGH
                     RLVVLCLRHHIPVVPIPGPSAFVAALAASGLPSDEFLFVGFLPPRAGARRKKLDALKD
                     EPRAIVLYEAPHRLAETLADAAEILGSRHAVVAREVTKIHEEFLRGTLPELRDAAKER
                     APRGEITLIIGPPEEGAMETVSAVSLKQRVSQLEAEMGLDRKAALKQAARERGLGKRD
                     AYKQLLLER"
     gene            9509..9832
                     /locus_tag="VG272_08975"
     CDS             9509..9832
                     /locus_tag="VG272_08975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HWF13853.1"
                     /translation="MPLYEYRCLKCDRHTDKIENVNGPHLKKCPHCGGKVESVITAPS
                     IQFKGSGWYVNDYGKKTSGGDSNKSDKSEKSEKTEKSEKSEKSESKNSSTKETKKTGS
                     VSKEK"
     gene            complement(10303..11196)
                     /gene="galU"
                     /locus_tag="VG272_08980"
     CDS             complement(10303..11196)
                     /gene="galU"
                     /locus_tag="VG272_08980"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HWF13854.1"
                     /translation="MTNTPKRVRKAVLPAAGLGTRFLPATKAQPKEMLPVVDKPLIQY
                     VVEECVASGIENIIIVTGRGKNAIEDHFDSSPELESFLEEKGKLDLAKLVHDIGNMVH
                     FSYTRQKEPLGLGHAVLVAKELVGNEPFAVLLGDVIIPGPNPATKQLVDVYQQTGKGA
                     IAVEEVPRDRTGLYGIVDVDQNAGIGGNKRTLNVRDLVEKPSPAEAPSTLAVTGRYLL
                     PPEIFDCLERTKPGRGGEIQLTDALRLLAKESGLLAYIYEGKSYDAGDKLGFLKATVE
                     IGLENCEFGKEFREYLRTLKL"
     gene            complement(11394..12950)
                     /locus_tag="VG272_08985"
     CDS             complement(11394..12950)
                     /locus_tag="VG272_08985"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWF13855.1"
                     /translation="MKILLYSPDNGVTKNFMPHLWMFLLQSLTPSGHEVVLIDGNAQP
                     MDEAGIAKYVRENNIGLVGIGCMTRMIAKAYRMADAVRAAGVPVVMGGPHVTEMPDEA
                     LGKKSGKRHADAVALGEADDTWPKIVDDAARGQLKEIYSPVDAFGQEKKPSLQDYPAI
                     PWDKIDLDQFNVVPKKLAPMLKRFRDGWGSFRIIPMESGRGCPYGCEFCTVTGFFGDS
                     IRFRSNKSVVDELLLLKERAKTEGGQIAVFFIDDNFAINPKRTKSLLRDIIAANAQVH
                     WVAQISANLLRDQELVDLISAAGGKWIFIGMESIDVANLADVNKGFNKPGEYAAVLQR
                     LADRNVFAITSFMFGMDNDTVGVAERTLQQIRTWPAGLPIFGLITPLPSTPLYARLEA
                     AGRLTRPKHWEEFIPFAMAHTPLKMSIEEAHAEVKYGWAKSYSPEAIALAVRSFNHKP
                     LAYRINILIARLCFRGIYFPQMGPLAWFKVIGENAQTIGDIVKEGFRDWFSGMCKRPA
                     NPTPAVSAGD"
     gene            13192..14337
                     /gene="tsaD"
                     /locus_tag="VG272_08990"
     CDS             13192..14337
                     /gene="tsaD"
                     /locus_tag="VG272_08990"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HWF13856.1"
                     /translation="MATRILGIESSCDETAAAIVVDGCEILSSVVSSQLDIHRKYGGV
                     VPELASREHLRQIVPVVREALLQAGLNFSDIDAVGVTQGPGLVGSLLVGVTYGKVLAT
                     ELKKPLVPVNHLEGHVHAVFLEAHLGAKVPQLPAVCLIVSGGHTLLYHVQCAAGNGDA
                     PKPGFQFRRLGGTRDDAAGEAYDKVARLLALGYPGGPIIDQLAGKVAASGSNFHEKDG
                     LNFNPVKIKGNPFDFSFSGIKTAVLYYVRQHPEFTEEIEIRKKALHSGLRKVTDLLPL
                     CSPRTLALISKFQAAVVRDLVTHALDAAVQTNSHTVLVSGGVAANSELRDRFTSKAAE
                     MGVTVFFPSRALSTDNAAMIAAAAYPHLLAGDLAANDLNAEPNLRLT"
     gene            complement(14446..14979)
                     /locus_tag="VG272_08995"
     CDS             complement(14446..14979)
                     /locus_tag="VG272_08995"
                     /inference="COORDINATES: protein motif:HMM:NF044704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HWF13857.1"
                     /translation="MDYKIGDKVVYPNHGVGMIEQINFGVMNGRTEKYFMVKILASGL
                     KVMVPATNAESVGLRHVIRNGETLKVLSFLEKGKPNSHHDWKHRFKENSERMRTGELI
                     EVAAVLKSLVSLSRTKPLSFREKKMLERAKFLLVSELATARNTSAETMEGALVKSLAK
                     ARLQMPSQMEKAEGMEM"
     gene            15200..15922
                     /gene="tsaB"
                     /locus_tag="VG272_09000"
     CDS             15200..15922
                     /gene="tsaB"
                     /locus_tag="VG272_09000"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HWF13858.1"
                     /translation="MLILAVDTSTRTGSAAILRDTEVLAEVSGYEETLYSNRLFRDIA
                     TLQERANFNLDQVDVFAVAAGPGSFTGLRVGLTAVKAWSEVHGKPIAAISGLEAIAYE
                     SLADMAPEALSASFIAPFLDARRGQIFGAVYKRGTGHDTELQLVSDESILSLDEYLDL
                     VKREAGQRVALVSPTPDVLPAARIEEVLPGQRTITVSAALAPAIGRLGFERARRGDLV
                     DAITLDANYVRRSDAEAAWKDG"
     gene            15922..16407
                     /gene="rimI"
                     /locus_tag="VG272_09005"
     CDS             15922..16407
                     /gene="rimI"
                     /locus_tag="VG272_09005"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HWF13859.1"
                     /translation="MFALGHKTWDPQSVAIRRMLASDASVAYSLLEESPEASMWSKES
                     LEESASQWVAWVAELDRQVVGFLAGRPVADEFEILNLAVGKNFRRNGIATKLLRSAIE
                     TARAAAATQIFLEVRPSNAPGIALYTHLAFRVCGRRKNYYRDPVEDALLLILRNFPKG
                     A"
     gene            16508..16744
                     /locus_tag="VG272_09010"
     CDS             16508..16744
                     /locus_tag="VG272_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13860.1"
                     /translation="MPALTREIREQLLTYDQEFQKLNQLHSEYETQLIQISHSPYLNL
                     EDLQQEATLKKLKLKVRDQMERHIAQRMNLRAAR"
     gene            16829..17440
                     /locus_tag="VG272_09015"
     CDS             16829..17440
                     /locus_tag="VG272_09015"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HWF13861.1"
                     /translation="MVKDGYRFAAPPLLLGIIAIVFHWNWLGGILIFLGLFVVFFFRD
                     PERTPPADPNLIVSPADGRVVGIVEESFGGSPGHRISVFLSVFNVHVNRSPVGGRITA
                     IEYRTGKFYAAMRGRASAENEQNSFHVLTDRGEVVFTQIAGWVARRIVVWKAIGDSLV
                     RGERIGMIRFGSRMDIWLPTEAEILVKSGQSVAGGTSVLARWK"
     gene            17437..18267
                     /gene="pssA"
                     /locus_tag="VG272_09020"
     CDS             17437..18267
                     /gene="pssA"
                     /locus_tag="VG272_09020"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HWF13862.1"
                     /translation="MSDFEQHELGFPGTRRASLKLRRGIYLLPSSMTVANMLCGFYAV
                     MSTVKGGSTDLDNAAKALGLAILFDSFDGFVARATGTSSEFGKQFDSLADMVSFGIAP
                     AVLAFVWGVRGIAETDFLDIRRLHEIGWWVVLTFVICCAWRLARFNIQGMAPGTGSKY
                     FVGLPCPAAAGLIAATVHALKTPILDWRWSIFWMFVVIADAALMSSTVRYRSFKDISM
                     AARQKSFLVIVMALLIWLIVTNSEIVLMIIATSFVASGIVLHLLRAGRRHTASHHANS
                     "
     gene            18264..19289
                     /locus_tag="VG272_09025"
     CDS             18264..19289
                     /locus_tag="VG272_09025"
                     /inference="COORDINATES: protein motif:HMM:NF014793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HWF13863.1"
                     /translation="MTPPKESVRIAIAGASSLLGKELSQWLEESNFPSVDIRLLDEEI
                     VAGTLTEAGGEPAVIETIDEDSFHRVRFAFFTGSPEFSQRHAAEAQRAGAVVIDLSGG
                     LGALASARLWIPSLDPILQSSPVVISPSEPQSIFVAPSAPSDAIISISAALAPAGLEH
                     LAVTVLQPVSERGTDAIEELESQVVKLLSFQPIPQGIFDAQVGFNMLSSFGPESRLKL
                     ADARTRISSESKRYLAGRAPVPAVMLVQAPVFHSHSFACYAEFKSAPALDDLAARLQS
                     AGLKVARAKDEPPTNVNVVAEARPVLRQPERDPSIETGVWLWGAVDNLRVPAATAVSI
                     AEKLLAS"
     gene            19457..20086
                     /gene="lptE"
                     /locus_tag="VG272_09030"
     CDS             19457..20086
                     /gene="lptE"
                     /locus_tag="VG272_09030"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HWF13864.1"
                     /translation="MKHFITARAKSAQFEISNFKIEIFALYICFATALFLTACGYHVG
                     GTGSQLPPGLKVIAVPAMKNDTTRYRVEQRMTEAVVHEFIARTKYRIVSSEDSADAVL
                     HGEITAFEAVPAVFDTTPASNPSTTNVPAINVTTARATTMLVSVHMKVLLEERDTKRV
                     LYKNDNYLFREPYEISTDPAKFFDEQGPALQRMSRDFASRLVADVLENF"
     gene            20086..21129
                     /gene="holA"
                     /locus_tag="VG272_09035"
     CDS             20086..21129
                     /gene="holA"
                     /locus_tag="VG272_09035"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HWF13865.1"
                     /translation="MPPLSPHELLARLAKGKPIPAILLAGSDSYLRDLCRKKIADTFV
                     AEGMRDWGIRKFSAEDDDLSAILGQAQTMPMLAPQQVIFASDVEAWERLGDDSRDAVV
                     KQISEYLDNPAPFTVLVFEAAALDQRMRLAKTFAEKAVTVSVELSTDSGERTRLAVPL
                     SIDMAKELGAELEHDAAEELCDLLGGELAAIHTEVEKLSAYAGDRKKITRADVDLLVI
                     SSQKYEVWDLADMLAERKAAQALEFLDSLLREGEAAVALLGGLAWMYRKLLEAQELPP
                     GTNGWQAASRLKMRAEAADLAMRRSRKFPKSQLTNGLAALYEADNRLKLGGYSQRAVM
                     EFLVTQLASTSAK"
     gene            complement(21300..>21536)
                     /gene="rpsT"
                     /locus_tag="VG272_09040"
     CDS             complement(21300..>21536)
                     /gene="rpsT"
                     /locus_tag="VG272_09040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HWF13866.1"
                     /translation="PVRAEINRANTTRVRTIMKKLRTAIAAKDSTSAGTLLRPTLSAI
                     DRAISKGVLEENTGNRYKSRLSIAVNGLSAAAKA"
BASE COUNT         4890 a         5847 c         5853 g         4946 t
ORIGIN      
        1 ttgctcggca ttaggcgtcg tgcggctaag cagttctaat caatggcgaa tcggattgta
       61 ttcgattttg atctggccct ggccatttca aaggcataga gcagctatag cagcaatcca
      121 cgtggtctga ccgaaggact aaaattggaa gattgcacga ccggcagcga taaagcgtct
      181 gtttatgaca aagtgggcaa aagaaaggag gtaaaccccc tccattctgc ggggtccata
      241 aataggggca atcttctccg gaacaaattg tgaaaggtcc catcaatacc tcgcttgaat
      301 gagcgtgcga agggttgtcc agaaatatcc agacgaacgg atcacgtgcc tggttcactg
      361 acgcgaaggg ggacatcaaa ttcccgggga acgggagggg ggttggttca ccgggagtga
      421 acaattcccg agaacgtcaa agctgatcgg aagcgttaca gcaccaaaag accaagttaa
      481 tttatagtca gagtcccgaa atgtgtcaag cacttaattc ttatttatct aacttattag
      541 tttattgtgt tccgtcccgg aactgatccg cgcagaagtt tagaagaaat tctactttct
      601 ctttctaaaa atctgcatct agtttgcgac tagtgcggtc aaacgttccc ctctccagcg
      661 tgatagtatg aagaagtcat tttccggcct gatgtttgtg aaaaaatgtg cacttgcttt
      721 ccccatggga aaaatagttt cgcaagaaga attgattcaa gtgacggggc gcgagagacg
      781 caacgggcgc acagttgtgt tcacgaatgg ctgctttgac ctgatccatc cgggccacgt
      841 acggtgtttg gcagaagccc gcgcgttggg cgatctgcta gtggtggctg tgaacagcga
      901 ccgcagcgtg cgcggcaata aaggaccgga gcggccgctg gtcgcggagc aggatcgcgc
      961 cgaagtgctg gccggactcg cctcggttga ttacgtcacc atcttcgacg agccgacgcc
     1021 ccgcgaactc attgcgcgtg tcctgccaag cattcttgta aagggcgcgg attgggccct
     1081 cgatcaagtg gccggacgcg aggaggtgga agccgccggc gggcgcgtga tctccattcc
     1141 gctggtgccc gggtactcca catcaaatct ggtgcaacga attcgtgatc tcggcggtgc
     1201 cgcaaccgta tcctcgcacg accctcactc taaatcatga ttctgtcatc cgtttcggaa
     1261 acgctagcct cgattggacg ccagtccgca ctggaattga ccatttcaga actgcgccag
     1321 ggcacagcgc tcgagcggct cgcaggattg acccaaacag gaaaagccct gattgcggca
     1381 atcattgcta cagaattgcg ccgtcccatg gtcgtactcg tcgaatccgg agcacgggcg
     1441 gaagagttcg ccgcgtccat gcaatttttt tacaaggcgc tcgccgggca ggaagccacg
     1501 gaagtaacca tactgcccgc attggatgta ttgccgtggc aggaagccgc cgcgcatgcg
     1561 gaaattctag aaacccgcgc ggtaactttg tggcggtacg ccaagggaga agcgcgcgta
     1621 gtggtcgcgc ccattgcggc ggcgcgcatg cggctcgcgg atgcgacgta ttacgcggag
     1681 caagcgcgca cgttggcgcg tgaagatcca ctttcactgg acgatcttgt agggcatctg
     1741 cgccgcgtcg gttacgaatc ccatgacatg gtggaaatgc ccggccaatt caccgtgcgc
     1801 ggcggaatcg tggatgtttt tccggccgaa gcggaacggc ccgtgcgcct cgagcttttt
     1861 gacgatacgg tggaatccct gcgcgagttc gatcctaata cgcagcgctc tgttcgtccc
     1921 atcgatcgcg ttacgcttcc tccgcttgtc gagcaatctg gaaatggttt tggtggacga
     1981 tacggcaaca cggaagacgc accgaaaatt tctcacacgc attctctgct cgatttgcat
     2041 gaggacaccg tcgtcctact cgatgaaccg ggagcaatcg aagaagcttc ccgcgcgttt
     2101 ttgcagggcg cgatggaaga cctcgaagcg aaaaaaagtg aatcgcttga tcttctatcc
     2161 ttacattact tcaccgataa ttgggacacg ctgcttgctc ggcacgcgcg actggaactc
     2221 gatcaactcg cccttacgcg cgaggattca gaaacgcgca tcatccagac gcagccgacc
     2281 tcaaacttcc acggcaatgt ccccgcgttt atggaggaag cccgcaaacg cgtggccgca
     2341 ggcgaacgcg tactactggc cgcggaaagc ctgggtgaac tggagcgcta cgtcgacctc
     2401 tgccacgaat acgaagtccc gtttcgtctt ggcgatctgg aagaaaacac gaccagcgcg
     2461 agattggcgg aagattcttc ggccggaagc gttccggcgg tcgtcctctg ccgcgcaccg
     2521 ttcacggatg gcgtaatttt tcttgacgcg cgagtcgcca tctttggcac gggcgatgtt
     2581 tttgaaagca tgcgcgcggg caccgccaag cgccgcccta agaccgcaag tttcttcagc
     2641 gatttctccg aactcaagcc gggcgactac gtggtccacg tggaccacgg catcggccag
     2701 tttgagggcc tgcatcagat cgaaaatggc ggcgcgcgcg gcgaattcat gcgcctgcgg
     2761 tatgcggacg acgcgcgtct gtatgttcca ctcgagcgcc tcgacctcgt gcagagttat
     2821 cgcgccgttg agggcgcgac gcctgtcctc gacaagcttg gcggcagcac gtggactgct
     2881 cgcaaagcgc gcgtaaaaaa atcggtcgaa gacatggccg agaaattatt gaaaatttat
     2941 gccggacgaa aaacggggga gggtattgcg ttttcggcgg atggtgagtt ccagcgcgaa
     3001 tttgaggatg ctttcgagtt tgaagaaacg ccagaccagg ctatcgccat cgctgacgtg
     3061 aagcgcgata tggagcgcca gcagcccatg gatcgtctgc tctgcggcga cgtgggctac
     3121 ggcaaaacgg aggtggccat gcgcgccgcg ttcaaagctg ccactgactc gaaacaagtg
     3181 gctgtgctcg ccccaacgac gatcctcgca tttcaacatt acgaaacatt tcgcaaacgg
     3241 tttgccgcat ttcccataaa ggttgatctg ctcagccgct tccgcaccgc acgcgaacaa
     3301 aaagatgttc tctcgctcgt tgaaacgggg caggtagaca ttgtcatcgg cacgcaccgg
     3361 ctgctttcaa aggacgtcaa gtttcatgac cttggcctgc tggtagtgga tgaagagcag
     3421 cgcttcggcg tggcgcacaa agagcgtctc aaggaactca agctgaacgt cgatgtcctc
     3481 acgctttccg cgacgccgat tccgcgaacg ctgaatatgt cactcatcgg cttgcgcgat
     3541 atgagcgtga tcgagactcc gccgaaagac cgaatggcga tccaaaccgt ggtcgctccc
     3601 ttttccgaat tgctgattcg ccgcgcgatt gatgaagaga tgttgcgcca cgggcaagtt
     3661 tttctggttc ataaccgcgt cgaaaccatt tatgccatcg ccgatctggt gaaaaaactt
     3721 gtcccaaaag cgcgtgtcgt ggttggccac ggccagatga acgaaaaaga attggaatcc
     3781 gtgatgttca agttcgtacg tggcgaggcc gatgttctgg tttccactac gattgtggaa
     3841 aatggcttgg acattcccgc cgcgaacacc attctcatca atcgtgccga ccgcttcggc
     3901 ctatcggagc tgtaccagct tcgcgggcgc gtaggccgct caaatcagcg tgcctatgcg
     3961 tatctgctcg tccctgagga tgtggtgctg acacaactcg cgacccggcg ccttgccgcg
     4021 ctaaaagaat tcagcgctct tggcgcgggg ttccgcgtcg ccgcgctcga tctggagctt
     4081 cgtggcgcgg gaaaccttct cggcggccag cagcacgggc acatcaatgc catcggcttt
     4141 gacatgtacg ttcaaatgct cgagcgcgct gtttcgacgc tgaagggtga agaagtgaag
     4201 cccgaaatgc gagccacgat caacctcggg ctcgacatcc gcatcccaga agagtacatc
     4261 ccgaacgaaa atcttcgttt gcggacttac aagcgcattg ccggcatcac caatgaaaag
     4321 gaaagggaag aggtcaacag cgagctgacg gatcgcttcg gcgctccgca ttcgtccgtt
     4381 ggcaatcttt tggattacgc gttgctcaaa ggattggcgg aaagtctgct gatttcgtcc
     4441 attgaaaggc gtgcgaacga agttgcaatc aaatttcatc ctgaaacgcc attgtcgccg
     4501 gaaaatctgg tgaaatttat ccgctcgcag aaggaaggcc tgcggctgga ccccagcggc
     4561 acgctttgga tgcgcttgga ccgccggggg cgcgtggcgg aaagcgtcag aaacgtgttg
     4621 cttccgctgg aaatgaagcg ctagactgga aaacgagcaa accctatgaa tttaagtaaa
     4681 tgcctgattt ttttacttgt ttttgcgagc ctggggattc ccgctggcgc gcaaaccaag
     4741 agcaccgtgg tcgaagaaat tgtcgcccgc gtgaacaacg atatcattac ccgtgaggac
     4801 ctggatcatg cgcacgcctc cctggatagc gaggctgcgg atgcgtgcca gaagtgcaca
     4861 cccgacgaag ttaaagcgat gatcgcgggg aaggaaaagg atttgcttcg cgatttgatc
     4921 gatcaatcgc tgcttacaca gcgcgccaag gatcagggaa tcaacgtcga cacggaagtc
     4981 atcaagcggc tcgactcgat ccgccagcaa tataaattgc ccgatctcga tgcactggaa
     5041 aaggaagtca cgaaaagcgg gcaggactat gaagatttta aaagccagct gcgcaatcag
     5101 ctcttgaccc aggaattaat gcgcaaggaa gtcggctcaa aaattatcgt cagccacgaa
     5161 gatatcgtga agtattacaa cgatcataag gccgaattcg ttcgtccaga aacggttgtc
     5221 cttcgcgaaa ttttcgtttc cacggaaggc aagcccgaag cagaccttcc agcgctaaag
     5281 aaaaaagcgg agggcttgcg cgacagggtg ttgaacaatg gcgacgattt tggtgaactg
     5341 gcaaaacact attccgacag cccgacggca cagcagtccg gagacctggg cacctttcaa
     5401 cgttcgcaac tggattccaa gatcgccgaa aaggtgtttg cactcaagcg cgaccagatg
     5461 accgacgtga tggaaacaaa aaccggctac gaagtcctgc aggttcgcga acgttacgat
     5521 gccggagaac agccactgga taaagtggaa ccggaaatca gcaataaact ttacgaacag
     5581 agcatggagc cggccatgcg cacctatctg acaacgcttc gtgaagacag tttccttcag
     5641 atcaagccgg gatacacgga cacggctgct gtaaaggccg aaccgattga ggaagttgcc
     5701 gccacgcctg ataaagacga taagaataag ggctcgcgta agtggggcgt gcttccgaag
     5761 aagaaatccg gcacatgaaa caatgcttcg tctccactct ccaggacgga cagaccgtta
     5821 cctcccattt tcttgtctgc gtaaaagaaa ttcgttccac gcgtgaaggc aaaacctatt
     5881 tacgtctgga gcttggcgat tcgactggac gcgtcgaagg gcgcatgtgg accgggtttg
     5941 acgcgatggc cgccacgttt gagcgcgatg attttgtaaa agtgcaggcg cgagtcgaga
     6001 attaccgcaa taaactgcag ttggccattg aaaaaattcg ccgcgctgaa gaaaacgaag
     6061 tcgatcccgc cgattttttc ccgcacacca gggaaaatgt cgaagagatg tacgcgaagc
     6121 ttctggaaat tgtcgcctcg gtagggaatc catggctgcg gcaacttctc gatagcgtgg
     6181 ttcaggatcc ggaaatcgtt ccgcgcctta agcgcgcacc cgccgcgaaa atcatgcatc
     6241 acgcttttta cggcggcctg ctcgaacacg ttctcagcct gtgcaatctt tgccgcgtgg
     6301 tcctcagcca ctatcccgaa gcaaatgcgg atttgctctt atccggcgcg gtcctccacg
     6361 atgttggaaa attgaaagag ttaagctacg accgctcgat tggctatacg gatgaaggcc
     6421 agcttctcgg ccacatcctg atcgagtacg agcatctcac caaaaaaatc gatgccatcg
     6481 aaggatttcc gtcggagctg aaaactttag tcctgcacat gttgatttcg catcacggcc
     6541 agtatgaatt tggctcgccg aaacttccca tgtttcgcga agcgttgctg ctgcattatc
     6601 tggatgatct cgattctaaa atggccgcga ttcgttccgc gctcgattcc gacgaaggcg
     6661 aagggaattg gaccgggttc aacggtgcgc tggcacggcg aattttgcgc gcggataaat
     6721 ttctaggagt tccggcggca gtaattccgg atgcgtccgc ggtcgccgaa tccgtagcca
     6781 gcagcgcgga ttccgcggat ggaaaaaagt agcggcatgg aacacatcga tcaaccacga
     6841 gctgcgggac cgcgccattc gattttggaa attgcgccgc tcgaactgga atgcctaagc
     6901 gtgctctggc cgttgggcga aggcactgtg cgcgatattc accgcgcgct cgccgcgtcc
     6961 cggccgcgcg cgtacaccac cgtaatgacc atcatggacc ggctcgaaca aaaaggaatt
     7021 gtggctcgcc gcaaagtggg ccgcgcgttt ctctaccagc cgaagctcag cgctgaggaa
     7081 gctcgcctga aagccgtcga aaaaattgtc gaaggatttt ttaacggctc gccggaagca
     7141 ctcgtggcgc atctttcttt tcgaggaaac gtggctgcac cgccgcccgt cccagcgact
     7201 gccagggttg atgtttcgtc cgcacctgta gcagaattac cctcccggaa tctcgatgaa
     7261 agattacttt aatagaatgc taccggcgtc cttttccatt cgcgataaaa acattgcacc
     7321 cggcacggtc ctggcaccga tggctggcgt gacggatacc gtttttcgcc gcatgattcg
     7381 cactctcggc ggctgcggcc tgatcatgac tgaattcacg agcgctgagt gcatcacgcg
     7441 caataacgct cgcacggctc gctatctttt ctttgcgccc gatgaacacc ccatcaccgg
     7501 ccagttgttc ggcgcggagc ccgaggtgat ggcccgcgct gccggaatgg tcgagcagct
     7561 tggttttgat atcgtcgacg tgaatctcgg ctgccctgcg agaaaagtgg tgaagtgcgg
     7621 cggttccgga ttgctgcgcg atttaaaagc cctggagcag gttcttagcg cggttcgcaa
     7681 cgccgtgaaa attccgatga ccattaagat tcgcgccggt tgggacgaaa acagcatcgt
     7741 tgccgtggaa gtcgcgcgcc tcgccgaaca aatcggagtg gagggaatcg cagttcatcc
     7801 gcggacacgc gcgcaaggat acacgggacg cgccgattgg aaagtgattc gcgacgtgaa
     7861 gcaggccgta cgcatgcccg tgaccggtaa cggcgatatc aattcgcccg aagacgcgat
     7921 gcgcatgctg cgggaaacag gatgcgatgc cgtaatgatc ggccgcgccg cttccaacaa
     7981 tccatggatt ttcaagcaga tttcgcagtt tgccgcgaca ggatcgtaca atcagccgac
     8041 cgagtgggac cgctaccagc tgctttccat gtatttccga aatttagtcg ataccgagtg
     8101 gccggatgca attggaagga tgaagcagtt caccagctac ttcacgcatg gtgtgagaaa
     8161 tggcggggag ttgcgcaaga acgttcacac ggcgcgtacg acctctgaaa tcctcgatcg
     8221 cgtggactcg ttcttcgccc aaaccgcaat ctgaaacgtg gcataggctt cagcctgtgg
     8281 gttttggtcc tagcaatatc caaattccac aggctgaagc ctatgccaca acttcccaat
     8341 cgcttatcgt tccagcaaaa gttgcttgta tgcgtcgcgc ttaccgagcc cgcgttcgcg
     8401 cgcagcctgc ttgagcgccg ctttccgatc gaggcccatc tccgcttcga gttgtgatac
     8461 gcgttgcttg agcgaaacgg cagagactgt ttccattgcg ccttcttcgg gaggtccgat
     8521 gatgagcgta atttcaccac gcggagctct ctcttttgcc gcgtcgcgaa gttcaggaag
     8581 tgttccgcgc aaaaactcct catgaatctt ggttacttcc cgcgcaacga cagcgtgccg
     8641 cgatccaaga atttccgctg catccgcaag agtttccgcc agcctgtgcg gcgcttcgta
     8701 cagtacgatg gctctcggct cgtccttcaa tgcgtcgagc ttttttcggc gcgcgccagc
     8761 acgcggcgga agaaatccca caaataaaaa ttcatcggaa ggcagtcccg aagcagccag
     8821 cgcggctaca aatgcggatg gccccggaat gggcaccacg ggaatgtgat gccgcagaca
     8881 caagacaaca agccggtgcc cgggatcgga tacgacagga gtccccgcat cgctgaccaa
     8941 cgcaacctgc gcgccttgct ccaactggat caccagttcc ggcgcgcgcg tcagttcatt
     9001 gtgcgcatgg tagctgacca tttctttatg gatcgaataa tgttgcagca atttctgcgt
     9061 ttgccgcgtg tcttcgcacg caatcaggtc tgcttccttg agcacgcgaa tcgcccgcag
     9121 cgtaatatct tcgaggttgc cgatcggcgt agccaccagg tacaggcacc ctgtttgcga
     9181 attgtcttgt gcgtccgtca ttttgctttc ctcgtgacgc cgagtttagc atgcggcttg
     9241 ccggagacca gtgttattga tggattgcgg ggatgggcgg gagcttcaat tgcggaaggg
     9301 aagccaaacg aaacaaatcg ccctctgcgt cccgaaaagg ccggaacgca gagggcgcta
     9361 cgaaaccgct ttcgtagcgg ccgtccgatt tttggacggg cgactttttt gctcaacgcg
     9421 ccgcattgcc cacaagcggc agaatcgcaa attcttgcac ccggcatgct aagatactcc
     9481 attccatctg aactgaagga gaccagcatt gccgctctac gaataccgtt gccttaagtg
     9541 tgatcgccac accgataaga ttgaaaatgt gaacgggccg cacctgaaga aatgcccgca
     9601 ctgcggcgga aaagtcgagt ccgttatcac tgcgccctcg atccaattca aaggttccgg
     9661 ttggtacgtc aatgattacg gcaagaaaac ttccggcggg gattccaata agtcggacaa
     9721 aagcgaaaaa tccgaaaaga cggaaaagtc cgagaaatcg gaaaagtcgg agtcgaagaa
     9781 ttcctctaca aaagaaacaa agaaaaccgg aagcgtatca aaagagaagt agttctgggt
     9841 agaacaggct tcagagtctg ggtgagaaat cgacaatcct tgtgggtcgt ggttttagcc
     9901 acgacataag tcctgcaaaa tgatgcggct ttacagtttg cggaaaaagt agtttattct
     9961 gtcattccga gcgaagcgag gaatctctct tcgatttaag tataagaaaa taaagaagga
    10021 gggattcctc gcttcgctcg gaatgacaaa acgttgagtt tttccgcagc ctctttattg
    10081 atgactgcat aactaatgtc cgggcacccc tgcccaaaag gcccctaaaa ggaccgaaaa
    10141 ctgacggtat gcataggaca ttagaaatgc agccctcaat agccgctaaa acttcaattt
    10201 tgcagttctc acagactctt cagtctgttt cttttgactt cgatcaaatt cagcaagaaa
    10261 ttccaaaaca ggcgagtcac gcagactctg aaaaaagccg ccctacaact tcaacgtccg
    10321 cagatactca cgaaattcct tgccgaattc acagttttcc agcccaattt ccaccgtggc
    10381 tttcaagaat ccgagtttgt ctcccgcgtc gtacgatttc ccttcataga tgtacgcgag
    10441 cagcccgctt tccttggcca acaatcgaag cgcgtctgtc agctggattt ctccgccgcg
    10501 ccccggcttt gttcgctcca ggcaatcgaa aatttcgggc ggcaatagat atcgcccggt
    10561 cacagcgaga gtggaaggcg cttcggcggg actaggtttc tcaacgaggt cacgtacatt
    10621 caaggttcgt ttgtttccgc cgatgcccgc attctgatcc acatcgacga tgccgtaaag
    10681 tccggttcta tcgcgcggca cttcttccac cgctatggcg ccttttcctg tctgctgata
    10741 aacgtcaacc aattgcttgg tcgcgggatt cggcccggga atgatgacat cgcccagcag
    10801 cacggcgaac ggttcattgc ccacaagctc cttggcgacg aggacggcat gtcccagtcc
    10861 cagcggttct ttttgccgcg tataactgaa gtgcaccata tttccaatat cgtgcactaa
    10921 tttggccaga tccaattttc ctttttcctc aagaaaactt tccagctcag gggaagaatc
    10981 gaagtggtcc tcgatcgcgt ttttgccgcg gcccgtgacg ataatgatat tctcgatgcc
    11041 cgacgcgaca cactcttcga ccacgtattg aatgagaggc ttgtccacca caggcagcat
    11101 ttcctttggc tgcgccttgg tggcgggtag aaatcgggtg cctagaccag ctgctggcag
    11161 aactgctttg cgaacacgtt taggtgtgtt ggtcatggat atagttaagt aattctcaaa
    11221 tccacattac gtgtgaaaat tgattcgtgt caatccatcc ttcgccttag aagttcctga
    11281 aacgtattct cttgtagtgt atgcacggga atggcggaaa ttgcgaacgg agatgcagct
    11341 gcctgcttgc ctgaatccta aaaactatga atcaacgtgc tcctggaacg gttttaatca
    11401 cccgcggaga cggcaggagt ggggttcgcg ggacgtttac acatcccgct aaaccaatcg
    11461 cggaaacctt ccttcacgat gtcgccaatg gtttgcgcat tttcgccaat gactttaaac
    11521 catgcgagcg gccccatttg cgggaaatag attccacgga agcaaagccg cgcaatcaga
    11581 atattaatcc ggtaagcgag cggcttgtga ttaaatgaac gcaccgccaa cgcgattgcc
    11641 tcagggctat aggatttcgc ccatccatat ttcacttccg cgtgcgcctc ttcgatgctc
    11701 attttcagcg gcgtatgcgc catggcgaag ggaatgaact cctcccaatg ttttggcctt
    11761 gtaagacggc ctgccgcttc aaggcgtgcg taaagcggtg tcgacggcag cggagtaatc
    11821 aatccaaaga tcggaagtcc cgcgggccac gtgcggattt gttgcagtgt gcgctccgct
    11881 accccgaccg tatcgttgtc catgccgaac atgaaagaag tgatcgcgaa aacgttgcgg
    11941 tccgccagcc tttgcagcac ggccgcatat tctcccggct tgttgaatcc cttattcaca
    12001 tcggcgaggt ttgctacgtc aatcgattcc atgccgataa atatccattt gccgccggca
    12061 gctgaaatga gatccacaag ttcctggtcg cgcaataaat tggcgctgat ttgcgccacc
    12121 cagtggacct gtgcatttgc ggcaataatg tcgcgcagca gtgacttggt tcgcttgggg
    12181 ttgatcgcaa aattgtcgtc gatgaaaaag acggcaattt gtccgccttc tgtttttgcc
    12241 cgctctttga gcagcaacaa ttcatccacc acgcttttgt tgctgcgaaa gcggatcgaa
    12301 tcgccaaaga aaccggtcac cgtgcaaaat tcgcatccat acgggcagcc gcggccagac
    12361 tccatgggaa tgatgcggaa cgatccccaa ccgtcgcgaa agcgcttgag catgggagca
    12421 agtttcttag gcacaacatt gaattggtcg aggtcgattt tatcccaagg aatcgccggg
    12481 taatcctgca ggctcggttt tttctcctgt ccaaacgcgt ccacaggaga ataaatttcc
    12541 ttcagctgcc cacgcgcggc atcatccaca atcttcggcc aagtatcatc tgcttcgccc
    12601 agcgcaacag catccgcgtg ccgcttgccg gatttttttc ccagagcctc atcaggcatt
    12661 tccgttacat gcggtccgcc catcaccacg ggcactcccg cagcgcgcac ggcatccgcc
    12721 atgcgatagg ccttggcaat catgcgcgtc atgcatccga tgccgacgag tccaatatta
    12781 ttttcgcgta cgtacttcgc gatgccagcc tcgtccatcg gctgcgcatt gccgtcgatc
    12841 aataccactt catggcccga tggagtgagc gactggagaa gaaacatcca caggtgcggc
    12901 ataaaatttt tcgtgacgcc gttgtccggg ctatagagca gtattttcat tttttcttct
    12961 ctgacgattc gccggaaaga caggatccga gttccggctt tcgtccaaaa ttcgttctga
    13021 agggtccgaa accgtcgccc aacggggcgc ggcggaggga tttgaagcga ccgcacggag
    13081 tctatcatca aaaatgcaca aaaaatgtca ttttattgta atgccacgga ctcgttaatg
    13141 ccggcttctg ttgtgttagt ataaaaaatc ccgaacgcga ggaaataatc aatggcaacg
    13201 cggatccttg gtatcgagtc atcgtgtgac gaaacagccg cggccatcgt tgtggatgga
    13261 tgcgaaattt tgtcgagcgt ggtgtcctcg cagctcgata ttcaccgaaa atatggcggc
    13321 gtagttcctg agctcgcctc gcgtgaacat ctgcgtcaaa ttgttcccgt ggttcgcgaa
    13381 gcgcttctac aggcaggctt aaatttcagc gacattgacg ccgtcggtgt aacgcaaggc
    13441 ccgggcctcg ttggatcgct tttggtggga gttacttacg gaaaagttct tgccacggaa
    13501 ttaaagaaac ctctcgtgcc cgtgaatcat ctcgaagggc atgttcatgc cgtgttcctt
    13561 gaagcgcatt tgggcgccaa ggtaccgcaa ttgccggctg tctgcctgat cgtttccggt
    13621 ggacacacct tgctttacca cgtgcaatgc gcggcaggga atggcgatgc cccgaagccg
    13681 gggtttcaat ttcggcgtct gggcggaacg cgcgatgatg ctgccggcga agcttatgac
    13741 aaagttgcgc ggctgctcgc gcttggctat ccgggtggcc cgatcattga ccagcttgcg
    13801 ggaaaagtag cggcctccgg ttccaatttt cacgagaaag acggcctgaa tttcaacccg
    13861 gtgaaaatca aaggcaatcc attcgatttc agtttcagcg gtatcaagac ggccgtgctg
    13921 tattacgttc gccaacatcc ggaatttacg gaagaaattg aaattcgcaa gaaagcgctc
    13981 cactcgggct tgagaaaagt aactgactta ttgccgctgt gcagcccgcg aacgctcgcg
    14041 ctgatttcca aattccaagc cgcagtcgtt cgcgacctgg taactcacgc actcgatgcg
    14101 gcggtgcaaa ccaattcgca caccgttcta gtttcgggag gcgtcgccgc gaattccgag
    14161 ttgcgcgacc gctttacatc caaagccgcc gaaatgggcg tcacggtttt ctttcccagc
    14221 cgcgccctct ctacggacaa cgctgcgatg atcgccgcgg ccgcctatcc ccacttgctg
    14281 gctggagatt tagccgccaa cgatctgaac gccgaaccaa atcttcgcct cacctgaagc
    14341 attgctaata gcgcgaaagc gtcagacatc gtcccaaaaa gaaaaaaccc caaaacagga
    14401 catgtgttgg ggttctttcg aactgtttta aaagtgaata tccagttaca tctccatacc
    14461 ttcagctttt tccatctgag agggcatctg caagcgggct ttcgccaggg acttgacgag
    14521 tgcgccttcc atggtttcgg ctgaggtatt tcgggctgta gcgagttcgc ttacgaggag
    14581 gaatttggct cgttccaaca ttttcttttc gcggaaggaa agcggcttgg tgcggctgag
    14641 cgataccagg gattttagta cggctgctac ttcgattagc tccccggttc gcatccgctc
    14701 cgaattttct ttaaagcggt gtttccaatc gtggtgcgaa ttcggttttc ccttttcgag
    14761 aaagctcaaa actttgagcg tttctccgtt tcgaatgacg tgccgcaatc cgacagattc
    14821 agcgtttgtg gccggaacca tgactttgag cccgctcgcc aaaatcttta ccataaaata
    14881 cttttctgtg cgcccgttca ttaccccgaa attgatctgt tcgatcattc ctacgccgtg
    14941 gttcggatag acaaccttgt cccctatctt gtaatccatg gtcggaaacc ccctagaggt
    15001 gagctgaagc aatactgaac cattcgatat ctgaagtcaa catatttctt taagaatcag
    15061 taagttatgc gattttttca tttgagattg gtcattgaat tgcacatgta ttttttttaa
    15121 tatttcaccg tagaaacttc tcgttttctc ttattcttcc ccggccagac tttaccctcg
    15181 attgcgctag aatagctaaa tgctgattct tgccgtagat acaagcactc gaacgggcag
    15241 cgctgcaatt ctgcgagaca cggaggttct ggccgaggtt tccgggtatg aagaaaccct
    15301 atattcaaat cgattatttc gcgatattgc cacacttcag gaaagggcaa atttcaacct
    15361 ggaccaagtc gatgttttcg ccgtggcggc cggtcccggt tctttcaccg gcctgcgtgt
    15421 cggcttgacc gccgtgaagg catggtcaga ggtccacggg aagcccatag ccgcgattag
    15481 tggcctggaa gcgattgctt acgaatctct ggcagacatg gcccctgagg ctctctcggc
    15541 ctccttcatc gccccatttc tggatgcccg ccgtgggcaa atcttcggcg cggtctataa
    15601 gcgaggcacg gggcatgaca cggaactgca actcgttagc gatgagtcga ttctttcgct
    15661 ggatgaatat ctcgacctgg taaaacggga ggcgggccag cgggtagccc ttgtgtcgcc
    15721 aacccccgat gttttacctg ccgctcgcat cgaggaggtc cttcccggcc agcgaaccat
    15781 taccgtgtcc gcggcgttag cccctgccat tggccgatta gggttcgaac gagccaggcg
    15841 tggagatttg gttgacgcaa ttacgctgga cgcgaattat gtgcgcagat ccgacgccga
    15901 agctgcctgg aaggatggat aatgttcgca ctaggccata aaacctggga ccctcaatcc
    15961 gtcgcgattc ggcggatgct ggcttcagac gcatcagtgg catattcctt actggaggaa
    16021 agtcccgaag cgtcgatgtg gtctaaagag agtcttgaag aatcggcctc tcagtgggtc
    16081 gcgtgggtgg cggaactcga ccgccaagta gtggggtttt tagccgggcg gcccgtggca
    16141 gatgaatttg aaatattaaa tctggccgtc ggcaaaaatt ttcggcgaaa cggaattgcc
    16201 acaaagctgc tccgtagcgc gatcgaaacc gctcgcgcag cggccgcgac gcaaattttt
    16261 cttgaggttc gcccttccaa tgcgcccgga atcgctctct acacccattt ggcgttccgc
    16321 gtttgcgggc gccgtaagaa ttattatcgc gacccggtag aagacgcttt gttattaatt
    16381 ttaagaaatt ttccgaaagg cgcttaattt ttagaagatt tttacttggc gggagattgg
    16441 cattgtgcta gcttcgcctt gttatgtaat accaccctca caattcggtt aaatcaggag
    16501 gcacattatg ccggctttga cccgtgagat acgcgagcaa cttctgacat atgaccagga
    16561 atttcagaag ttgaatcaac tgcactcaga atacgaaact cagcttatac agatctcgca
    16621 ctccccctac ctgaatctgg aagaccttca gcaagaagct accctcaaga aactgaaatt
    16681 gaaagttcgg gaccagatgg aacgtcatat cgcgcaacgc atgaatcttc gcgccgcgcg
    16741 ctaacactcc agtccgccgc caaactgata tagaatattt gtggacgcta cgggagtgcc
    16801 gttatttttc ctcctgacaa ggacgtgcat ggttaaggac ggctataggt ttgccgcacc
    16861 gccgctgctg cttggcatca tcgcaatcgt atttcactgg aattggcttg gcggtatttt
    16921 gattttcctg ggacttttcg tggttttctt tttccgcgat ccggaaagaa cgccgcctgc
    16981 ggatccgaat ttgatcgttt cgcccgcgga tggacgcgta gtggggattg tggaagaatc
    17041 ttttggcggc agtccgggcc accgcatcag cgtgtttctt tctgttttca acgtgcacgt
    17101 gaaccgctcg cctgtcggtg ggcgcattac cgccattgag taccgtaccg gcaagttcta
    17161 cgcggccatg cgcggacgtg cttcggccga aaatgagcag aattccttcc acgttttgac
    17221 cgatcgcgga gaagtggtgt ttacgcaaat tgccggatgg gtggcgcgcc gtattgtcgt
    17281 ctggaaagcg attggcgatt ccctagttcg aggagagcgc attggtatga tacgcttcgg
    17341 ttcgcgcatg gacatttggc tgcccactga agccgaaatt ctggttaagt ccggccaaag
    17401 tgtggctggc ggtacaagcg tcctggcgcg ctggaaatga gcgacttcga acagcatgaa
    17461 ctaggattcc ccggcacgcg gcgagccagc cttaagctgc gtcgcggcat atatcttttg
    17521 cctagttcga tgaccgtggc caacatgctc tgcggcttct acgcagtgat gtccacggtc
    17581 aaaggcggta gcacggacct ggacaacgca gcgaaggcac tcggcctcgc aatcctgttc
    17641 gattcctttg acggcttcgt ggctcgcgct accggcacca gcagcgaatt cggcaagcaa
    17701 tttgattcgc tagcggacat ggtcagcttt ggcatcgctc ccgccgtgct ggcgtttgtg
    17761 tggggagtgc gcggcatcgc cgaaaccgac tttctggata tccgccgcct gcatgaaatc
    17821 ggttggtggg tggtgctcac gtttgtgatt tgctgcgcct ggcgcctcgc tcgattcaac
    17881 attcagggca tggctcccgg aaccggttct aagtatttcg ttggcctgcc ttgtccggcg
    17941 gctgcaggat tgattgcggc caccgtgcat gcgcttaaga cccccatctt ggattggcgc
    18001 tggtccattt tctggatgtt tgtggtcatc gccgacgccg ccctgatgtc cagcaccgta
    18061 cgctaccgca gctttaagga tatttccatg gcggcgcgcc aaaaatcgtt tctggtcatc
    18121 gtcatggcgc ttttgatttg gctgattgtg acgaattctg aaatcgtgct catgattatc
    18181 gccaccagct ttgttgcctc cggaattgtt cttcatcttt tgcgagcggg tcgccgccac
    18241 acagcctcgc atcacgcgaa ttcatgacac cgcctaaaga atcggttcgc atagccatcg
    18301 ctggcgcatc ctccttgctt ggcaaggaat tgagccagtg gcttgaggag tccaattttc
    18361 cttccgtcga tattcgcttg ctggacgaag aaatcgtcgc cggaactttg accgaagccg
    18421 gtggggagcc cgccgtcatt gaaaccattg acgaggatag ctttcaccga gttcgcttcg
    18481 cgttcttcac cggatcgccg gaattttctc agcgccatgc cgcggaagcg caacgagcag
    18541 gcgctgttgt gattgatctc agcggagggc ttggcgcgct cgccagtgcc aggctttgga
    18601 ttccctcgct agacccaatt ttgcaatcgt cgccggtggt aatatcgcca agtgagccgc
    18661 aaagcatttt cgtcgctcca tccgctccgt cggacgcgat aatttcgatt tccgcggcgc
    18721 tcgcccccgc gggcctcgaa cacctcgccg tgaccgtact tcaaccggtc tccgagcgcg
    18781 gcacggatgc gattgaggaa ctcgaaagcc aggttgtaaa actcttgtcg tttcagccta
    18841 ttccgcaagg gatcttcgac gctcaggttg gctttaatat gctttccagc ttcggcccgg
    18901 aaagcagact caagcttgcc gatgcccgta ctcgcatttc cagcgagtcg aaaagatatc
    18961 ttgcagggcg cgcgcccgtt ccggccgtca tgctagtgca ggctcctgtt tttcactccc
    19021 attccttcgc atgctacgcg gaattcaaat cggctcctgc gttggatgat ctggcagcgc
    19081 gcctgcaaag cgcaggctta aaggtggcta gggccaaaga tgagccaccc acaaatgtga
    19141 acgttgtggc ggaggcgcgc ccggtcctca ggcaaccgga gcgcgaccca agcatcgaaa
    19201 cgggtgtctg gttgtggggt gctgtggaca atctgcgggt cccggctgcc accgccgtct
    19261 ccatagcgga aaaattgctg gcttcgtaat gtaagcccgt tttgagtggc gcgggcttca
    19321 gcctgcggcc gttagtgcct gcaaggctta ccccgcaggc tgaaggcccg cgccactgaa
    19381 atacaaatca gacctggaag tactcagatc gttttgagct gggcgaaagc gaagttggag
    19441 taaggaatat tctgaagtga aacacttcat cacagcccga gcaaaatccg cgcaatttga
    19501 aatttcaaat ttcaaaattg aaatattcgc gctatacatt tgctttgcga cagccctctt
    19561 tctcaccgcg tgcggctatc atgtgggcgg aacaggttcc caacttccgc ccggacttaa
    19621 agtaatcgct gtccccgcca tgaagaatga cacgacgcgt taccgcgttg aacagcgcat
    19681 gactgaggcg gtggtccacg aattcattgc ccgcacgaaa tatcgcatcg tttcctcgga
    19741 agattccgcg gacgcggtgc tgcacggtga aatcaccgcg tttgaagcgg tccctgctgt
    19801 ttttgacacc acgcccgcgt caaatcccag cacgacgaac gttccggcga tcaacgtcac
    19861 cacggcgcgc gccaccacca tgctcgtttc cgtgcacatg aaagttttgc tcgaagagcg
    19921 cgacacgaag cgagtgcttt acaaaaacga taactatctt ttccgcgagc cttacgaaat
    19981 ttccaccgac ccggcgaaat ttttcgatga gcaaggtccc gcgctgcaaa gaatgtcacg
    20041 cgatttcgct tcgcgcctcg tagccgacgt tctggagaat ttctaatgcc tccgctttcg
    20101 cctcatgaac ttctggcgcg cctcgcaaaa ggaaagccca tccccgcaat tctgcttgcg
    20161 ggcagcgaca gctatttgcg cgatctttgc cggaaaaaaa ttgccgatac gtttgttgcc
    20221 gagggaatgc gcgattgggg aattagaaaa ttttccgctg aggatgacga tctatccgcg
    20281 attctagggc aagcgcagac catgccgatg ctcgcgccgc aacaagtgat tttcgcaagt
    20341 gacgtggagg cttgggagcg attgggtgac gattcacgcg atgcggttgt gaaacagatc
    20401 tctgagtacc tggataatcc tgcgcctttc accgtactcg tgttcgaggc cgccgcactc
    20461 gatcaacgca tgcgtctcgc caaaacgttt gcggaaaaag cagtgacggt ttccgtcgag
    20521 ctttccaccg attccggtga acgtacgcgt ctggctgtgc ccctgtccat tgacatggct
    20581 aaagaattgg gagccgagct ggagcacgat gcggccgaag agctttgtga cctcctgggt
    20641 ggcgagctgg ctgcaattca taccgaagtc gaaaaacttt ccgcctacgc aggcgatcgt
    20701 aaaaagatta cgcgcgcgga tgtggacctc ctcgttattt cttcgcagaa atatgaagtg
    20761 tgggatttgg cggatatgct ggccgagcgc aaggcagcgc aagcactcga atttctggac
    20821 agcctgcttc gggaaggcga agcggctgtg gcccttctcg gcggcctggc gtggatgtat
    20881 cgaaagcttc tggaagcgca ggaattgccg ccggggacga atggttggca ggctgctagc
    20941 cgtctgaaaa tgcgtgcgga agctgcggac ctggccatgc ggcgatcgag aaagtttccg
    21001 aaatcgcaac tgacgaacgg cctggctgcg ttgtacgaag ccgataaccg cttgaaatta
    21061 ggcggataca gccagcgcgc cgtcatggaa tttttagtta cgcagctggc gtccacttct
    21121 gccaaatagt cctgcaatca acccaacctg gagaagtggt ttatgtggca taggtttcag
    21181 cctgtggaat ttaggccttg cgaggactaa accccacagg ctgaagccta tgccacaaaa
    21241 aaacgcccga caatttcggg cgtcttgtct agatttatca agatttttag cagctgaaac
    21301 tacgcctttg cagcggcgct caatccattg acggcaatgc tcaatcgcga tttgtagcga
    21361 ttcccagtgt tctcttcgag cactcccttg ctgatcgcgc gatcaatcgc ggacaacgtc
    21421 gggcggagca gggttccagc cgatgtggaa tccttcgcgg caatcgccgt gcgcaacttc
    21481 ttcatgatgg ttcgcacccg agttgtattc gcgcgattaa tttccgcgcg taccgg
//
LOCUS       DASZMB010000196         8220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2677178, whole genome shotgun sequence.
ACCESSION   DASZMB010000196 DASZMB010000000
VERSION     DASZMB010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8220
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2677178"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..811
                     /locus_tag="VG272_09045"
     CDS             293..811
                     /locus_tag="VG272_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HWF13867.1"
                     /translation="MNHVTERNNEIPSLYEWLGRADVLNTLMVKFYERVSGDELLRPL
                     FGEMPVDHPRHVADFVGEVMGGSEAYTASRGGHATMLEHHFGRNISEAQRRRWVSLLL
                     DSADEVGLPGDPEFRSALVAYLEWGSRLAVMNSKLPSGTVADSATTMPKWGWGVPGGP
                     YVPPSDGKESKS"
     gene            complement(862..936)
                     /locus_tag="VG272_09050"
     tRNA            complement(862..936)
                     /locus_tag="VG272_09050"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(902..904),aa:Lys,seq:ctt)
     gene            complement(1006..1971)
                     /locus_tag="VG272_09055"
     CDS             complement(1006..1971)
                     /locus_tag="VG272_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13868.1"
                     /translation="MANPLKVRLCFHDRCFDGAASAAVFYRFFRERFLPDAEFHFTGM
                     AHQARNPFPEALFDGDENAIVDFKYSSSPRVTWWFDHHQSAFLTPADAENFRQDRSGH
                     KFYRPEYKSCTKLLADVTSAQFGFDVTPLAELIHWGDIIDGAQYATPESAVAVAEPAT
                     KLALVIESAPEEGLVSKIIPDLATRPLAEMIELPIVARHLGPLLEKHRQSIDLLKKRI
                     EFRGGVLYFDITDLPIDGYNKFIPYMLYPDARYTVSVLSSPSRAKISIGSNPWNPDAA
                     EDNLASLAEQYGGGGHPRVAAISLDPNDVERAKQVARDIAEKLRD"
     gene            complement(2118..2600)
                     /locus_tag="VG272_09060"
     CDS             complement(2118..2600)
                     /locus_tag="VG272_09060"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HWF13869.1"
                     /translation="MAFCSKCGTEVGAGAAFCPKCGAAQNSGPAPTGGAVAEPVQATQ
                     PGMSENVGGLLCYLLGWVTGIIFFLIDKRPFVRFHAAQSMVTFGGLHILNIVLGIIFG
                     AGMMFRGGYGAFGMGAALYSLIGLVTFVLWILLMVKAYQHEKFEVPIVAGIAKSFSGT
                     "
     gene            3445..4566
                     /locus_tag="VG272_09065"
     CDS             3445..4566
                     /locus_tag="VG272_09065"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWF13870.1"
                     /translation="MKKFLILIVLAASLAAMGCAHENLNPQAGAPPPAQVEHEQDGDV
                     IQVEHSEQIPLAVAVVHNSHPEIVLTGAVTPDVSRNVPVVSLASGRVVGVRARLGDTV
                     RKGDLLLQIRSDDVAGGFSDYRKAIADEKLTSAQLDRAKDLYVHKAISLNDLQIAEDA
                     EEKASVDVETKADHLRLLGNDPNTPNNFVEIHAPVSGVITDQQVTEGASVQAFGPNPF
                     TISDLSTIWVICDAHEDQLAELRVGDTAKIRLNAYPSQIFKGTVNNIGAVLDPNLRTA
                     KVRMDVRNPGMMRVGMFVTATFRSQKQVAHTAIPSTAILHLHDKNWVFAFTPDKKFHR
                     VEVQSGIALPGDLQEINSGLAPEAQVVLNALTFQNTVEQ"
     gene            4568..7705
                     /locus_tag="VG272_09070"
     CDS             4568..7705
                     /locus_tag="VG272_09070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HWF13871.1"
                     /translation="MIRKLVDFALENRFMVLAAALLLFGWGAISFHQLPIEAYPDVAD
                     NYVEIITQWPGISAEQIEQQVTIPLEIAMNGIPHVAHLRSFSLFGLSDLKLVFDDEEE
                     NAWNRERVLERLSQVTLPPGVAPQMGTDWSPVGQVYFFTLKSSNPQYDVMDLKSIEDW
                     VVEKNLKSVPNVVDVSSFGGPTREYQVRLDPEKLISYGLSIAQVEQQLANNNTNAGGS
                     FIETGQQQINIREVGLVRNVGDIENTVLAARNGTALRVKDIAVVAQGPKIRLGQFARA
                     IHREDGKIIDNDDVVSGIVLLRKGANADDALAGIHEKVAELNNRILPSGVKIVPFIDR
                     SDLVHFTTHTVLHNLTEGIILVVIVLFLFVGNVRAAIIVALTIPFALLFAATCLNLKG
                     VPANLLSLGALDFGMVVDGAVVMIENIVRHMTHPKNSERPPMEIIREAAHEVQRPVFY
                     AIAIIITSYLPIFTLQRVEGRLFRPMAWTVAFALLGALIFSMLIAPVLSTLFFRNGVK
                     EWQNPAMTYLTQGYRKAVRWAIEHRKVTVGMGVAGLLVAIFLVYGGMLGSEFLPHLDE
                     GAVWARGTLASSIGPSEAIKVANEARVKLCSFPEVTECTSQVGRPDDGADATGFFNTE
                     YFVGLKPKEEWRPGFAQDKDKLIAAMNREVEKIPGVTWGFSQPIEDNMEEAVSGVKGE
                     LATKVYGDDLKVLEEKADRIVNAMQQVKGIEDLGVFRALGQPNINFAVDREKAARYQI
                     NVADIQDAIQTAVGGTALTQVLQGEQRYDLVLRYLPQYRTTKEALEDIRLLSPSGERV
                     SLAQLCTIQEQDGGSEIYREENQRYVAIKYSVRGRDLGSAVEEAMRKVNAQVQLPRGY
                     HIDWEGEYESQKRAQARLLIIVPLTILVIFMIIYSMFRSPKWAALILATVAMASVGGL
                     LALLITGTHVSVSSGVGFLALFGVSVQTGVIMLEYINQLRARGGSIEESAVDGAVLRL
                     RPIMMTMLVATLGLLPAALSHAIGSDSQRPFAIVIVGGLMVNLAMSIFILPTLYVWLA
                     REGDKLPEPEPNFEP"
     gene            7977..8063
                     /locus_tag="VG272_09075"
     tRNA            7977..8063
                     /locus_tag="VG272_09075"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8011..8013,aa:Leu,seq:tag)
BASE COUNT         1936 a         2136 c         2243 g         1905 t
ORIGIN      
        1 aaattgggat tgccgatgtt attgacctca gttgcgacat gattgatggg ccggcgtctg
       61 tctgtaccct gttccaaaac ctggtaatgg ttttttggag cgggatggaa ggatcacctg
      121 agtatctgta gacgctcagg atttcgtctc cctaatggca acggttgtgc ccttgccagc
      181 tatgtgcgaa attaaggttt ttaggcattt acggatccgc ccactaaggt ctgaatctga
      241 tgcacgattg ggtcgaggat gttttgaacc tttttgcggc gaagaaggga aagtgaatca
      301 cgtgaccgag agaaataatg aaattccgtc attgtatgag tggctgggta gagcggacgt
      361 cctgaatacg ttgatggtga agttctatga gcgagtatcc ggtgacgagc tgttgcggcc
      421 tttgttcggc gaaatgccgg tagaccaccc tcggcacgta gcggattttg tgggcgaagt
      481 gatgggagga tccgaggcat atacggcgag ccgcggcggc cacgccacca tgttagaaca
      541 tcactttggg cgaaacattt ctgaggcgca gcggcgacgg tgggtgagct tgcttcttga
      601 cagcgcggat gaggttgggc ttccaggcga tccagagttt cgatctgcgc tcgtggcgta
      661 tttggagtgg gggtcgcgac tcgcagttat gaattcgaag ctgccgtcgg gtacggttgc
      721 ggattcagcc acaaccatgc cgaagtgggg ttggggagtg cccggtgggc cgtacgtgcc
      781 cccttccgat ggaaaagagt ccaaatcgta gaacgaaacc aagtccccag gccctcggaa
      841 accatataaa cattaatgat ttggtgaacc gggtaggact cgaacctaca acccgctgat
      901 taagagtcag ctgctctgcc attgagctac cggtccacga actttttatt ttagcatgac
      961 tcgacgacgt tttcggctgc cgatcacgcg cgcggcgcaa aaaaattaat cgcgtaattt
     1021 ttcggcgata tccctggcca cttgtttggc gcgctcgaca tcgttcggat cgagcgaaat
     1081 tgcggctacg cggggatggc cgccgccgcc gtattgttcc gcgaggctgg ccagattatc
     1141 ttccgcggca tccgggttcc acggattcga gccgatggaa attttcgcgc gtgaaggcga
     1201 agaaagcaca ctcacggtgt aacgcgcatc cggatacagc atgtacggaa taaatttatt
     1261 gtagccatcg atgggcagat cggtgatatc gaaatacagc acgccgccgc gaaattcgat
     1321 gcgttttttc agcaggtcaa ttgactggcg atgcttttcg agcagcggac ctaggtgcct
     1381 ggcaacgatc ggcaactcga tcatttccgc aagcggacgc gtggccaggt ccggaataat
     1441 tttggataca agtccctctt ccggtgcgga ctcaatcaca agcgccagct tcgtcgcagg
     1501 ctcggccacc gcgaccgcgg attcgggcgt cgcgtattgc gcgccatcga taatgtcgcc
     1561 ccaatgaatc aactcggcca gcggcgtcac gtcgaaaccg aattgcgcgc tggtcacgtc
     1621 ggccaatagc ttggtgcagg atttatattc ggggcgataa aatttatggc cgctgcggtc
     1681 ctggcggaaa ttctcagcgt ccgcgggagt caggaatgcg ctctggtgat ggtcaaacca
     1741 ccaagtcacg cgcggcgagc tcgaatattt gaaatcgaca atggcgtttt catcgccatc
     1801 gaacagcgct tcggggaaag gattccgcgc ctgatgggcc atgcccgtaa aatgaaattc
     1861 agcgtccggt agaaagcgtt cgcgaaaaaa gcggtagaaa accgccgcgg aagccgctcc
     1921 atcgaagcat cggtcatgaa aacacagccg gacttttagc ggattcgcca ctctactctt
     1981 ggtccccatc ccacaaatca aggcaatttg aaagccctaa aagatggctg ggaccgggac
     2041 gaatgtcaag agtgaaaagt tggaatcgct gattccgcta aagttggaaa catcaaattt
     2101 caagaaatcg gacgcgctta cgtgccactg aagctttttg caatgccagc cactatcggc
     2161 acttcaaatt tctcatgctg atatgccttc accataagca aaatccagag gacgaaggta
     2221 accaagccaa tgaggctgta caacgccgcg cccattccga aagctccgta gccgccgcga
     2281 aacatcattc ccgcgccaaa aataatcccc aggacaatgt tcaggatgtg aagaccgccg
     2341 aacgtgacca tcgattgcgc cgcatggaat cgaacaaacg ggcgtttgtc gatcaaaaag
     2401 aaaatgatcc ccgtgaccca gcccaacaga taacacaata atccgccaac gttttcagac
     2461 atgccaggct gagttgcctg cacaggttcc gctaccgctc cgccagttgg cgcaggtccg
     2521 ctattttgtg ccgctccgca ttttgggcag aaggctgcac ccgctcccac ttccgtaccg
     2581 cacttgctac agaacgccat aggatttctc cccctctcga ggtggtgaag gaagtactac
     2641 ccgccattgg ccgaaggcta tcaccgtcgc ccccgccgca caagctttac gggaaattta
     2701 catcgaataa aagagtctcg ggtaaagatt gccctgagat tgcgcggcat tgtaaactgg
     2761 atcgcgtatt caacgatttt ctcgcgttac tttttgtagt cagggacgcc ttgctcgcgg
     2821 tgccttgagg cttgcgtcag caataaaata aaaatggaaa cgactcttaa tctcatttgg
     2881 gtggccgtaa ccattgcggc agtttggctg tggcgattcc actggagtgt ctgccgtgaa
     2941 aatcggaaac acagcacacg gatgcaatcg attgcgatgg tctgtgtctt ggcactcctg
     3001 ttccctgtca tttccctcac ggacgatctg caccctgaaa cagtggctgt ggacgcggct
     3061 tcaggcaaaa gaaatgcttg cctgatcgct gccagttgcc cccacgctcg aaatgcaacg
     3121 ctcagattcg gaaagcattt tacgttaggg ttgatttcca ggcccttcgc tacagcaaat
     3181 ttagcaatca ctgagtttct tgccgatgct gattttggta aaatcagtcc tatagttgac
     3241 aattcccgcg gtcgatctcc ccctatactc taaatagtcc aattttcaac tttttctgtt
     3301 tccttgcctg atttaggtct gggacgcagt actttctcca atgtactcgt cccaaaattc
     3361 cgcgcattcg aattcgcgtc tttggccttt acagatgagt cgaataaaaa gttggcgcgc
     3421 gtacgcttga atagagaggg agaaatgaag aagttcttaa ttttgattgt gttagccgcg
     3481 tcgctggccg cgatgggctg tgcacatgaa aatttaaatc ctcaagcggg tgcgcccccg
     3541 ccagcccaag tggaacacga acaggatggc gacgtcattc aagtggaaca ttcggagcag
     3601 attccgcttg ctgtggcggt tgtgcacaat tctcatccgg aaattgtcct caccggagcg
     3661 gtgacgcctg acgtttcgcg caatgtgcca gtcgtctcgc tggcttcggg ccgcgttgtg
     3721 ggggtccgcg cgcgccttgg tgacacggtt aggaagggcg acctgctttt acaaattcgg
     3781 agcgacgatg tagccggcgg gttttcggac taccgcaagg cgattgccga tgaaaaactg
     3841 accagcgctc aattggaccg cgcaaaagac ctttacgtcc ataaagcaat ttccttaaac
     3901 gatcttcaaa tcgcggaaga cgcagaagaa aaagccagtg tggacgtcga gacgaaagcg
     3961 gaccaccttc gcctgctggg caatgatccc aacacgccga ataatttcgt ggaaattcat
     4021 gccccggtct caggcgtaat caccgatcaa caagttaccg agggcgcgtc cgtccaggcg
     4081 ttcggtccaa acccattcac catttcggat ttgtcaacca tctgggtcat ctgcgacgca
     4141 catgaagatc aacttgccga actgcgagta ggcgacaccg cgaaaatccg cttgaacgcc
     4201 tatcccagtc agattttcaa aggcacggtc aataatattg gggccgttct ggatccgaat
     4261 cttcggacgg ccaaagttcg catggatgta cgaaatcccg ggatgatgcg cgtcgggatg
     4321 ttcgtgacgg ccacgttccg aagccagaag caggtggcgc acacggcgat tccctcgacg
     4381 gcgatcctcc atcttcacga taagaattgg gtttttgcat ttacccccga taaaaaattc
     4441 caccgcgtgg aagtgcaatc cggcatcgcc ttgccgggag acttgcagga aatcaactct
     4501 ggccttgcac cggaagcgca agtcgtcctt aacgcattga cgtttcagaa caccgtggag
     4561 cagtaagatg attcgcaagc ttgtcgattt cgcgctcgaa aatcgcttta tggtcctggc
     4621 cgccgccttg ctgctgtttg gttggggcgc aatctcgttc catcaacttc cgatcgaagc
     4681 gtaccccgac gtggcggaca actatgtgga aatcattacg cagtggccgg gcatttccgc
     4741 cgagcagatc gagcagcagg tcacgattcc cctcgaaatt gcgatgaatg gaattcctca
     4801 tgtcgctcat ttgcgttcgt tctcactttt cggactctcc gacctgaaat tagttttcga
     4861 tgatgaagag gaaaacgcgt ggaaccgtga aagagttctt gagcggcttt cgcaagttac
     4921 cctgccgccc ggcgtagctc cgcagatggg aacggattgg agccccgtcg gacaggtcta
     4981 cttctttacg ctgaaaagca gcaatcccca gtatgacgtg atggatttga aatcgatcga
     5041 agattgggtc gtcgaaaaga atttgaaatc ggttccgaat gttgtggacg tttccagttt
     5101 tggcggccca acgcgcgagt atcaggttcg tcttgatcct gagaaactga tctcctacgg
     5161 cctcagcatc gctcaggtag aacaacagct cgcgaataac aatacgaatg cgggcggaag
     5221 tttcatcgaa accggccagc aacaaatcaa tattcgggaa gtaggcctgg tcaggaatgt
     5281 cggcgatatc gaaaacacgg tcctggcggc caggaacggc acggcgctcc gggtaaaaga
     5341 tattgccgtt gtggcgcaag gtccgaagat tcggctgggc caattcgcgc gagccatcca
     5401 ccgcgaggat ggcaagatca tagataacga tgatgtggtc tcagggattg tgctcctgcg
     5461 aaaaggtgcg aatgccgatg acgccctcgc ggggattcac gaaaaggtag cggagctgaa
     5521 caatcgtatt ttgccttccg gtgtaaaaat tgttcctttc atcgaccgca gcgatctggt
     5581 gcatttcacc actcacacgg ttttgcataa cctcacggaa ggaatcattc ttgtcgtcat
     5641 cgtcttgttt ctttttgtgg ggaatgtgcg cgccgccatc atcgtcgctc tgacaattcc
     5701 cttcgcactc ttgttcgctg caacttgcct taacctcaag ggtgttcccg caaacttgct
     5761 ctcgctgggc gcgctcgatt tcggcatggt cgtggatggg gcggtggtca tgatcgagaa
     5821 catcgttcga cacatgaccc atccgaaaaa ttccgagaga cccccgatgg aaataatccg
     5881 cgaagccgcg cacgaggtgc aaagacctgt tttttatgcc atcgcgatca ttattacctc
     5941 ctacctgcct atctttacgc tgcagcgcgt ggaggggagg ctcttcaggc caatggcctg
     6001 gacagtagcg tttgctttac tcggtgcgct gattttttcg atgctgatcg ctccggttct
     6061 ttccacgctg ttttttcgca atggcgtaaa ggaatggcaa aaccctgcga tgacctattt
     6121 gacccaaggc tatcgtaagg ccgtacgttg ggccatcgaa catcgcaagg tgaccgtggg
     6181 gatgggagtg gctgggctac tcgtggccat ttttctggtc tatggcggca tgctcggttc
     6241 cgagtttttg cctcacctcg acgaaggcgc cgtctgggcg cgcggaactc ttgcatccag
     6301 tatcgggccg agcgaagcga tcaaagtggc caacgaagct cgcgtaaagc tgtgttcttt
     6361 tcctgaagtt acagaatgta cgagtcaagt agggcgcccc gacgatggag cggacgcgac
     6421 cggttttttc aacacggaat atttcgtggg actgaaaccg aaggaagagt ggcgacccgg
     6481 gttcgctcaa gacaaggata aattaattgc ggcgatgaac cgcgaagtgg aaaagattcc
     6541 cggcgtaacg tggggatttt ctcagcccat cgaagacaat atggaagaag cggtcagtgg
     6601 cgtaaaaggc gaactcgcca ccaaggttta cggagatgac ctgaaagttc tcgaggaaaa
     6661 agccgatcgg attgtgaatg cgatgcagca ggtcaaggga attgaagatc tcggcgtttt
     6721 tcgcgccctg ggtcaaccga atatcaactt tgcagtggac cgggagaaag ccgcgcgcta
     6781 tcaaattaac gtcgccgaca ttcaagacgc aattcaaacc gcggtgggag gcacggctct
     6841 cacccaagtg ttgcaaggtg aacaacgcta tgacctcgtt ctgcgttatc ttccgcaata
     6901 ccgcacgacg aaggaagctc tggaagacat tcggctttta tcgccgtccg gagagcgagt
     6961 ctcactcgcg caactctgca cgatccagga acaggacggc ggctctgaaa tttatcgtga
     7021 agaaaaccaa cgctacgtgg cgatcaaata cagtgtaaga ggtcgcgacc tcggtagtgc
     7081 cgtcgaagaa gcgatgcgca aagtaaacgc gcaggttcaa ctgcctcgcg gttaccacat
     7141 tgactgggaa ggcgaatacg aaagtcagaa acgcgcccag gcgcggctgc tgattattgt
     7201 tccgctgacg attctggtga ttttcatgat tatctattcc atgttccggt ccccgaaatg
     7261 ggccgcgctg attctggcca cggtggcgat ggctagcgtc ggcgggctgc tggccctgct
     7321 catcacggga acacacgtaa gcgtttcctc aggagtggga ttcctggcac tattcggcgt
     7381 ttccgttcaa accggcgtca tcatgctcga atacatcaat caacttcgcg cgagaggcgg
     7441 atcgatcgag gaatccgcgg tcgatggcgc tgtcctgcga cttcgcccca tcatgatgac
     7501 catgctggtg gctacgcttg gcctattgcc ggctgcactt tcgcatgcga tcggttccga
     7561 ttcgcagagg cctttcgcaa ttgtgatcgt gggaggcctg atggtgaatc tggcgatgag
     7621 catttttatt ttgccgacgc tttacgtctg gctagccaga gaaggcgaca aactgccgga
     7681 accggagccg aacttcgaac cgtaagtgcg ttgcatgttt cttgtccaat cggcgcgtcg
     7741 tcagaagaaa gttaaaagat ttaacacaga ggacacagag aaaagaggaa aaccacagag
     7801 agatgtcggc tcatagatta ggaatttatt tctcccggtt ttctccgctt ttcctctgtg
     7861 tcctctgtgt taaatctttt gaccttgcga tctgcacgcc atatctcgtt ccgaaaatcg
     7921 ccagcaatcg ccagcaatcc tcaagcatcc accgaagtgc tatactgtcg cgccgagcgg
     7981 aagtggcgga attggcagac gcgccagcct taggagctgg tggccgaaag gccgtagggg
     8041 ttcgagtccc ttcttccgca ccaccaaata catattttcc agctgctgca acgtttatcg
     8101 cggcgcaaaa tcctgcgcag gattgtagcg gcaaacaaaa gggcgcgaag ggtcaaaaag
     8161 attagccttt cattcagcac ttccttgcag gacatctctc gccacgaatc gcggaaaact
//
LOCUS       DASZMB010000197         3764 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2119807, whole genome shotgun sequence.
ACCESSION   DASZMB010000197 DASZMB010000000
VERSION     DASZMB010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2119807"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..371)
                     /locus_tag="VG272_09080"
     CDS             complement(30..371)
                     /locus_tag="VG272_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13872.1"
                     /translation="MKNLDIDSLTEEDLVELNHKIVARLRFLSQMRAHSAMLDFRIGE
                     RVKFSPGGRLEVFGVLTQYNKKTVTVITDSGEHWNVAPGLLKKVESAQAESARKDGIK
                     LVHHSNRSSEQ"
     gene            535..984
                     /gene="rplM"
                     /locus_tag="VG272_09085"
     CDS             535..984
                     /gene="rplM"
                     /locus_tag="VG272_09085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HWF13873.1"
                     /translation="MRTYVPRGRDTEIIEQGGGWFVLDASGQVMGRLATRIARMLIGK
                     DKANYTPNLDCGDHVVVVNADKIRLTGNKLDQKIYRHHSGYPGGLKEVPAKRLRPTRA
                     DWMLREAVLGMLPKNKLRALRAKKLRIYLDDKGLARHAGQKPQAAAL"
     gene            1137..1556
                     /gene="rpsI"
                     /locus_tag="VG272_09090"
     CDS             1137..1556
                     /gene="rpsI"
                     /locus_tag="VG272_09090"
                     /inference="COORDINATES: protein motif:HMM:NF001099.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HWF13874.1"
                     /translation="MSSSGQPVEGAKQKFYGTGRRKESVARVFLTFGSGQITINGRTP
                     DEYFRNVTWRNRAVEPMKFTETSGSVDVIATVNGGGVGGQSGAVRMGLSRALAVFNPE
                     LRAGLRKNGFLTRDSRMKERKKYGQKGARKRFQFSKR"
     gene            1719..2606
                     /gene="rpsB"
                     /locus_tag="VG272_09095"
     CDS             1719..2606
                     /gene="rpsB"
                     /locus_tag="VG272_09095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HWF13875.1"
                     /translation="MAVEITMKELLEAGVHFGHQTRRWNPKMKEYIFGERNGIHIIDL
                     QKTLKLFREAARFVSEQSGSGKTILFLGTKRQAQESVAEEANRCGMFYVNHRWLGGTL
                     TNWSTLQKSIKRLKTLKAMTEDGRMAQLSKKEAARLDRELKHLKQNLSGVENMSALPD
                     AMFVIDSNAEGIAVREARRMGVPVVSIVDTNCDPDQVDWVIPGNDDALRAIKLFTSKI
                     SDAVIEGRQAYERSQIAQEKAATDAATGADNVEYVDTSKYESYEKQEGDFIEPGTEPQ
                     AEALPEAELEPNQSVKAAE"
     gene            2703..3359
                     /gene="tsf"
                     /locus_tag="VG272_09100"
     CDS             2703..3359
                     /gene="tsf"
                     /locus_tag="VG272_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HWF13876.1"
                     /translation="MTNPTESVNISAQLVKELRERTGAGFSDCRSALVESKGDIEGAI
                     DVLRKKGQAAAQKKAARQASEGLVGHYIHAGGKIGVLVEINCESDFVARTEDFQRLCH
                     DVAMHIAALDTRFVRREDVTQEILDREREIYKAQARATGKPEAVAEKIVNGKMEKFYE
                     ENCLYEQHFIKDESITVGDLVSQMIHKLGENISIRRYVRFKVGESASSAADTAAAATS
                     "
     gene            3469..>3764
                     /locus_tag="VG272_09105"
     CDS             3469..>3764
                     /locus_tag="VG272_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13877.1"
                     /translation="MSDDDRKFQPIVTAPGLVYTRIVLKLSGESFQGSQGFGIDAETV
                     HNIAREVKEVNDLGVQMAIIVGGGNIFRGTRQKSLSIDRATGDYMGMLSTVINGL"
BASE COUNT          902 a          992 c         1074 g          796 t
ORIGIN      
        1 ccgtccagtt tttcggcgac ctattgtatc tattgttcac tactgcgatt ggagtggtgg
       61 acgagtttaa tcccatcttt tcgtgcggat tctgcctgcg cggattccac tttcttcaac
      121 aagcctggag caacgttcca atgctccccg gaatccgtga tcaccgtgac ggttttcttg
      181 ttgtactgtg ttaatacgcc gaagacttcc agccttccac cggggctgaa ttttactcgc
      241 tccccgattc ggaaatcgag catggcgctg tgcgcgcgca tttgacttag aaatcgcagc
      301 cgtgccacaa ttttgtgatt caactcgacc aggtcttcct cggtaaggga atcgatgtcc
      361 aagtttttca ttgtcgtttt catcgcctga gacctgccaa ttttgcacct gttgtttgcc
      421 tttggcaaca tcaagtcgtt ccgctggcgg ctatgatacg ctttgaattg acaaactgat
      481 tcatacccga taacctctac gtttgactat tcatggaaag gtgtgactaa gcacatgcgc
      541 acgtatgtac cccggggacg ggacacagaa attatcgaac agggtggcgg ctggtttgtt
      601 ctggatgcct ccggacaagt gatgggacgt ttggccacgc gcattgcgcg aatgttgatc
      661 ggcaaggaca aggccaacta cacgccgaat ctggattgcg gcgatcacgt tgtggttgtg
      721 aacgcggaca agattcgcct gactggaaac aagctcgatc agaagattta tcgccatcac
      781 agcggctatc caggcgggct gaaggaagtt ccggccaagc gcctgcggcc aactcgcgcg
      841 gattggatgc tgcgcgaagc tgtgctgggc atgctcccga agaacaagtt gcgggcgctg
      901 cgcgcgaaga agctgcgaat ttatctggac gataaaggtt tggctcgcca cgcggggcaa
      961 aaaccacaag cggctgctct ttaatagatt ggctttttca cgatccccgt ttcgttcaag
     1021 acgaaattac tgggatttgg atttctgctg cgcatccggc agcctcgtac tagaattgtc
     1081 agtagatggt cattagatgg ataccggaga tggatactag gatttgagga ggaaagttga
     1141 gttccagcgg acagcctgtc gagggcgcga aacagaaatt ttacggaacc ggccggcgga
     1201 aagaatccgt cgcgcgcgtg tttttgacct tcgggtctgg gcagatcacg atcaacgggc
     1261 gcacgccgga tgaatatttc cgcaacgtga cgtggcgcaa tcgcgccgtc gagccgatga
     1321 agtttaccga gacttcggga tcggttgacg tcattgccac cgtgaatggc ggcggcgttg
     1381 gcgggcagtc gggagctgtg cgcatgggtc tttcccgcgc gctggctgtg ttcaatcctg
     1441 agctgcgcgc cggtttgcgc aagaacggtt tcctgacgcg cgattcacgc atgaaggaac
     1501 gcaaaaagta cgggcagaag ggcgcccgca agcgcttcca gttcagcaag cgctaacgca
     1561 acgagttctg gtttcttccg tctcgcattg cgggacggaa agaactaaat tcacgcgccg
     1621 gcaaggagcc ggcaatagga tgttcagttc cggccctctt tttctcaatg aaagagtcgg
     1681 tacggaacgc gcaaacatct atctcctgag gaggaacatt ggcagttgaa ataaccatga
     1741 aagagttgct ggaggctgga gttcatttcg gccaccagac ccgccgttgg aatccgaaga
     1801 tgaaggaata tatcttcggc gaacgaaatg gaatccatat tatcgacttg cagaaaacat
     1861 taaagctgtt ccgtgaagcg gcgcgttttg tgagcgagca gagcggaagt ggcaaaacaa
     1921 ttttattcct gggaaccaag cgccaggcgc aggaatccgt agccgaggaa gccaatcgct
     1981 gcggaatgtt ctacgtgaat catcgctggc tgggcggtac gctgaccaac tggtccacgc
     2041 ttcagaagtc gatcaagcgt ctgaagacgc tgaaggctat gaccgaagac ggccgcatgg
     2101 cgcagctctc caagaaggaa gctgcccgcc tcgaccgtga attgaaacat ctgaaacaga
     2161 atctttcggg cgtggaaaat atgtccgcgc tgccggatgc catgttcgtt atcgattcca
     2221 acgccgaagg tattgcagtg cgcgaagcgc gccgcatggg cgttcccgtc gtttccatcg
     2281 tggacacgaa ctgcgatcca gaccaggtgg actgggtcat tccgggcaac gacgatgcgc
     2341 tgcgcgccat caaactcttc acgtccaaga tttccgacgc ggtgattgaa ggccgccagg
     2401 cgtatgagag atcgcaaatc gcccaggaaa aggctgcgac ggatgcggca acgggcgcgg
     2461 acaacgtcga gtacgttgac accagcaaat acgaatccta cgagaagcag gaaggcgatt
     2521 tcatcgagcc gggcacagag ccgcaggccg aagccttgcc ggaagctgaa ctcgaaccga
     2581 atcaatctgt aaaagccgcc gaataaggca aagtttcaga tcccgctcgt gtaacgccgg
     2641 gtatggctgt gcgttttttt ggtgcaaaga attgtcgata gataaaggaa tgccatagag
     2701 ccatgacaaa tccaaccgaa tcggtcaata tttcggcgca acttgtaaag gaacttcgcg
     2761 aacgtaccgg cgcgggattt tccgactgcc gctcggcgct cgtagaatca aagggcgaca
     2821 tcgaaggcgc gattgacgtc ctccgcaaga aggggcaggc cgccgcgcag aagaaagcgg
     2881 ctcggcaggc cagcgaaggc ctagtggggc actacattca cgccggcggg aaaatcggcg
     2941 tgctcgtcga aatcaattgc gagtccgatt tcgtcgcgcg caccgaggat ttccagcgcc
     3001 tgtgccacga cgtggccatg cacatcgccg cgctcgatac tcgatttgtc cgccgcgagg
     3061 atgtcacgca ggaaattctg gaccgcgaac gcgaaattta caaagcgcag gcccgcgcca
     3121 caggcaagcc tgaagccgtc gccgaaaaaa tcgtgaacgg aaaaatggaa aagttctacg
     3181 aggaaaattg cctctacgag cagcacttca tcaaggatga gtccatcacc gtgggcgacc
     3241 tggtcagcca aatgatccac aaactcggcg aaaacatttc cattcggcgc tatgtgcgct
     3301 ttaaagtggg cgagagcgcg tcgtcagcgg ccgataccgc cgccgcggcc acctcctagc
     3361 cattcgcaac cgggagcgta aatctcacga cgcggtcgtc acagccgcga tgtcgggaag
     3421 cagggagtac ggtctccata gatatttgct agaatacccg ccgagcgtat gagcgacgac
     3481 gaccgaaaat ttcaaccgat agtgaccgca ccgggacttg tttatacccg gatcgtatta
     3541 aaactttccg gcgaatcctt tcaaggttcc caaggcttcg gcattgacgc cgaaaccgtc
     3601 cacaacattg cccgcgaggt aaaggaagtg aacgatctcg gcgtgcagat ggctatcatc
     3661 gtgggcggcg gaaatatttt tcgcggcacc cgccaaaaat ctctatccat cgatcgcgcc
     3721 accggcgact acatgggtat gctttccacg gtgatcaacg gact
//
LOCUS       DASZMB010000198         5631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3749629, whole genome shotgun sequence.
ACCESSION   DASZMB010000198 DASZMB010000000
VERSION     DASZMB010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5631
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3749629"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2597)
                     /locus_tag="VG272_09110"
     CDS             complement(<1..2597)
                     /locus_tag="VG272_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13878.1"
                     /translation="MTAEEQRLDESRQRTKHWKRWGPYLAERQWGTVREDYSPGGTAW
                     EYFPHDMARSRAYRWGEDGIGGISDRHQRMCFALALWNGRDHILKERLFGLTGNEGNH
                     GEDVKEYYFYLDSTPTHSYMKFLYKYPQAAFPYDQLVEENHKRSKREPEFELMDTGVF
                     NENRYFDVFMEYAKAEPEDILIKITAWNRGPEEAQLDLLPTLWFRNIWSFGEKHGHPN
                     LARVADFKGAAVIAAEESRYGKRWLLVENAPEMLFTENETNFARVFGFPDSNPYVKDG
                     FHEYVIGGVKEAVNPAQKGTKCAAHYRFKIAPGASVTVRLRLTNIEPKDSAANPFDDS
                     FEKILILRHRDADQFYARRFPQERSEDAHGVMRQALAGMLWSKQCYHYDVRTWLQGDP
                     GQPPPPPERKNGRNHDWAQLYNADVLSMPDKWEYPWFAAWDLAFHTVALSLVDPDFAK
                     DQLILLLREWYMHPNGQLPAYEWAFGDVNPPVHAWAAWRVYKIEKRIHGRADRAFLER
                     VFHKLLLNFTWWVNRKDPDGMNVFQGGFLGLDNIGVFDRSQPLPTGGHLEQSDGTSWM
                     GMYCLNMLAIALELAREDPAYEDVASKFFEHFVYIADAINSHGTENKSLWNDEDGFFY
                     DAIHLPDGEQRSLKVRSMVGLIPLFAVETLEPELIARLPGFQRRMQWFLNNMPGFSRH
                     VDQSQSSPNGVRRLLSLVDGGQLVRILSYMVNPQEFLSPHGIRALSKYHAEHPYILPV
                     NGNEHRVDYEPGESQSGLFGGNSNWRGPIWFPVNFLMIESLQKFHHYYGDDFRVECPT
                     GSGQQKSLWDVSAELSRSLTHIFLRDEKGRRPVYGANEKFQNDPLWRDLILFYEYFHG
                     DTGAGLGAS"
     gene            2876..3127
                     /locus_tag="VG272_09115"
     CDS             2876..3127
                     /locus_tag="VG272_09115"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HWF13879.1"
                     /translation="MKQMRASAFKARCLRVMNEIQATGEPVIVTKRGKPVVKVIPIKP
                     DNDDIFGFMAGKAEIIGDIESPVVPLKDWKIFKTKKVKK"
     gene            3198..3587
                     /locus_tag="VG272_09120"
     CDS             3198..3587
                     /locus_tag="VG272_09120"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWF13880.1"
                     /translation="MILLDTHALIWLAFEPAKLSKAAREAIHQSARTGGLGISAITLW
                     EAAWLVTHRRVDFTGTPEAFLEEISSRTAVFPITSEIAILANQLPATYSSDPSDRLIG
                     GTALAESIPLITKDRSIRNHKQIKTIW"
     gene            3667..4119
                     /locus_tag="VG272_09125"
     CDS             3667..4119
                     /locus_tag="VG272_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13881.1"
                     /translation="MKLNFIVVVPIVSAIVAAIVSLSIVFLTRRSETIKHLQSLRIAA
                     YVDFIRAVAGLAVVSRDAVQSREHFAKDWELRMLLADAKSRIAIYGSESVVGSLAEFL
                     RNGNVLNTPERARAFTAVCQNMRGDTRPKLETISQSDMHFLLFDDEIR"
     gene            complement(4237..4701)
                     /locus_tag="VG272_09130"
     CDS             complement(4237..4701)
                     /locus_tag="VG272_09130"
                     /inference="COORDINATES: protein motif:HMM:NF023093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YhaV family
                     toxin"
                     /protein_id="HWF13882.1"
                     /translation="MEVNGWRLFRYPLFESQLKKLTEAVERLSLSHPDTYKEHPKTKL
                     LATIYRTITETIPRNPNASEFRQGDTLGPDNRHWFRAKFHQHYRLFFRFSTKDKVIVY
                     VWVNDESTLRKAGSKTDAYAVFKSMLNAGEPPRTLEALLKHAKEIIGRSDMK"
     gene            complement(4701..5060)
                     /locus_tag="VG272_09135"
     CDS             complement(4701..5060)
                     /locus_tag="VG272_09135"
                     /inference="COORDINATES: protein motif:HMM:NF027267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
                     /protein_id="HWF13883.1"
                     /translation="MAKVRDSGVFFGKQAKTGNSLGLRFERALFHSHPEFSGEVEAHI
                     IGPGRMLVVADPKPKRKYAEEDPVLSSFMSFLAADMLRSPEQLTPLDAGLMERIGALV
                     SDVDTAPEEDLGNEALI"
     gene            complement(5223..>5631)
                     /locus_tag="VG272_09140"
     CDS             complement(5223..>5631)
                     /locus_tag="VG272_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HWF13884.1"
                     /translation="ARGIEVVEVRSARRAKYTNSLGVALRKDGETVSVLGMIGPNDAL
                     RILGVNNIDIDAPLTGFLLLFRNQDVPGVIGRVGTLLGKHEINIANFALGRVQNSDEA
                     IGIVNVDHTVPPDVLGELRAIAAIRQARVIEIR"
BASE COUNT         1291 a         1612 c         1434 g         1294 t
ORIGIN      
        1 tggctcgcgc caagccccgc tcccgtatcg ccgtgaaaat attcgtaaaa caaaattaga
       61 tcgcgccaca aaggatcgtt ctgaaatttt tcattcgcgc catacactgg ccggcgccct
      121 ttttcgtccc gcaaaaatat atgggtcagc gagcgcgaaa gttccgcgct cacgtcccac
      181 agcgactttt gctgccccga tccggtcggg cattccacgc gaaaatcgtc gccataatag
      241 tgatgaaatt tttgcaacga ttcgatcatc agaaaattaa ccggaaacca gatcggcccg
      301 cgccagttgg aattcccgcc gaacaatccg ctttgcgatt ctcccggctc gtaatccacg
      361 cggtgttcat tgccgttgac gggaagaatg tacggatgct ccgcatgata tttcgagagc
      421 gcgcgaattc catgcggcga caaaaattcc tgcggattca ccatgtagct caaaatccgc
      481 accagttgtc cgccatccac caatgacagc aaccggcgaa ctccattggg cgaagactgg
      541 ctctgatcca cgtgccgcga aaaccccggc atattattga gaaaccattg catgcgccgc
      601 tgaaaccccg gcaaacgtgc aatcaattcc ggctccagcg tctccaccgc aaacagagga
      661 atcaatccga ccatcgagcg cactttcagc gagcgctgtt ctccatccgg gagatgaatc
      721 gcatcgtaaa aaaatccatc ttcgtcattc cacagcgatt tattttcggt cccgtgtgag
      781 ttaatcgcgt ccgcgatata aacgaaatgc tcaaaaaatt tactggccac atcttcgtaa
      841 gcgggatctt cgcgagcgag ttccagcgca atcgccagca tattcaggca atacattccc
      901 atccagcttg tgccgtccga ttgctcgaga tggcctcccg tgggcagcgg ctgcgagcga
      961 tcgaacacgc ctatattgtc caggcccagg aaaccgcctt gaaacacgtt cattccatcc
     1021 ggatctttgc gattcaccca ccacgtaaaa ttcagcagaa gcttgtgaaa cacgcgttcc
     1081 aaaaaagcgc ggtcagcgcg cccgtgaatg cgcttttcaa ttttgtacac gcgccacgca
     1141 gcccatgcgt gcaccggcgg attcacatcg ccaaacgccc attcgtaggc aggcaactgc
     1201 ccgttcggat gcatgtacca ctcccgcagt agcaggatga gctgatcctt ggcgaagtcc
     1261 ggatcgacga gcgacaacgc aaccgtgtga aacgccaaat cccacgccgc aaaccacggg
     1321 tactcccatt tatccggcat ggaaagaaca tccgcgttgt acagctgcgc ccaatcgtga
     1381 ttgcgcccat ttttgcgttc tggcggcgga ggcggctgcc cagggtctcc ctgaagccat
     1441 gtgcgcacat cgtagtggta gcactgcttg gaccacagca tgccggcgag cgcctggcgc
     1501 atcacgccgt gcgcatcctc ggaacgctcc tgcggaaaac gccgcgcgta aaattggtcg
     1561 gcgtccctgt ggcgcaggat taatattttt tcaaatgaat cgtcgaacgg atttgccgcg
     1621 ctatcttttg gttcgatgtt cgtcaaccgc agtcgaaccg tgaccgaagc gcccggcgca
     1681 atcttgaaac gataatgtgc cgcgcatttc gttccctttt gcgcggggtt taccgcttcc
     1741 ttcacgcctc caatcacata ctcatggaag ccgtctttca cataagggtt actgtccgga
     1801 aaaccgaaca cgcgagcaaa attcgtttca ttctccgtga aaagcatctc cggcgcattt
     1861 tccaccagca gccagcgctt gccgtagcgg ctctcttccg ctgcaatgac tgccgcgccc
     1921 ttaaaatccg caacgcgcgc aaggttcggg tgtccgtgct tctctccaaa cgaccagata
     1981 tttcgaaacc acaaagtagg cagcagatcg agctgtgctt cttccggccc gcgattccag
     2041 gctgtgattt tgattaaaat atcttcgggc tcggccttgg cgtactccat gaacacgtcg
     2101 aaatagcgat tttcgttgaa cacccccgtg tccatcaatt caaattccgg ttcgcgcttg
     2161 cttctcttgt gattttcttc gacgagttga tcatagggga atgcggcctg cgggtacttg
     2221 tacaaaaatt tcatgtacga atgggttggc gtggaatcga gataaaaata atactccttc
     2281 acgtcctcgc catgatttcc ttcgttgccc gtcagcccaa acaagcgctc cttgaggatg
     2341 tggtcgcgcc cattccaaag cgccagagca aagcacatgc gctggtgccg gtcgctgatc
     2401 ccgccaatgc catcttctcc ccagcggtag gcgcgcgacc tcgccatgtc atgcggaaaa
     2461 tattcccacg ctgttccgcc cggactgtaa tcctcgcgaa ccgttcccca ctgtctttcg
     2521 gcaagatacg gtccccagcg cttccagtgc ttcgtgcgct ggcgcgattc atccagacgc
     2581 tgttcttcgg ctgtcatcga ttgttcctca ctccggtcct ttgaggggat acagccggca
     2641 ggtccgggga aatataagta taggcagaag aagcggcgag aggaaagaca agttagtgtg
     2701 catgttcgga agctctagcg cacacgaagc cggggctgct gtagcccgcc tcttcagagg
     2761 cgggggtttt ctgcgccgac aaagcacgat gtcccggcaa aggaaatccc tcgtcaccgt
     2821 catatccttt ccaaacaggt catgttaata tgaccatagt cacggagaag actccatgaa
     2881 gcaaatgcgt gccagcgcct tcaaagcccg ctgcctgaga gtaatgaacg aaatccaagc
     2941 caccggcgaa ccggtaatcg tcacgaagcg cggaaaaccc gtcgtgaaag tcatcccgat
     3001 aaagccggac aatgacgata ttttcggctt catggcggga aaagctgaga ttattggaga
     3061 tatcgagtct ccggtagttc ccttgaaaga ttggaagatc tttaagacca aaaaggtaaa
     3121 gaaatgatcc gcctcggcag caaagctcga tgctacggcg taccggtcgg ccatagccgt
     3181 ttcgcaggcg acggccgatg attcttctcg atacccatgc cctgatatgg ctcgcctttg
     3241 aacccgctaa attatcgaag gcggcaagag aggccattca ccaaagcgcc cggacgggag
     3301 gattaggaat ttccgcgatt acactctggg aggccgcctg gctggtgact caccgccgtg
     3361 tcgattttac tggcactccc gaggcgtttc tcgaggaaat ttcgtcgcgc acggcggtgt
     3421 ttcccattac ttcggagatt gctatcctgg cgaatcagct tcccgcaacc tattccagcg
     3481 acccgagcga cagactaatt ggagggaccg cactcgccga aagtattcct ctaatcacaa
     3541 aagaccgctc gatccgcaac cataagcaga tcaagacgat ttggtaagat acacttggac
     3601 ttacgaccga cgaggaccaa aagacccacc atgctgaaac tgctatggag ctctaaggcg
     3661 aaaataatga agctcaactt tatcgtggtc gtgcctattg tttctgcgat cgtcgccgca
     3721 attgttagtc tttcaattgt gttcctgact agaaggtcag aaacgatcaa acatttgcag
     3781 tccctaagaa tagctgctta tgtcgatttt attcgcgccg tggctggctt ggctgtcgtg
     3841 tcgcgcgacg ccgtccaatc tagagagcat tttgcgaaag attgggagct acgaatgttg
     3901 ctcgcggacg cgaaatcacg aattgccatc tacggtagcg aatcggttgt cggctcactg
     3961 gcagagtttc ttcgcaatgg aaatgtgttg aacacgccag agagggcaag agccttcaca
     4021 gccgtctgtc aaaatatgag gggcgacacc cgtccgaagc tcgaaacgat ttctcaaagt
     4081 gatatgcact tccttctgtt cgacgatgag atcagatagt atccaaaatg gtcgggtagc
     4141 catgcattgt cttgattttt cgggtgccgc actcttgcag ctcttgcaag ggtgcggttc
     4201 gtgaacttaa cttccccgca tcccgcaggg aaaactttac ttcatatcgc ttcttcctat
     4261 tatttccttc gcgtgcttca gcaaagcttc gagcgtgcgg ggcggttcac ctgcgttcag
     4321 catcgacttg aaaaccgcgt atgcgtcagt cttggaaccg gccttccgca gagtagattc
     4381 atcgttcacc cacacgtaga cgatcacctt atccttggtg gaaaagcgaa aaaacaggcg
     4441 atagtgctga tgaaatttcg ctcgaaacca gtgccggtta tcgggcccca atgtatcgcc
     4501 ttgccgaaac tcggaagcgt ttggatttcg tggaatagtt tccgtgatcg tgcgataaat
     4561 cgtggcgaga agtttggttt tgggatgttc tttgtaggta tccggatggc tgagcgaaag
     4621 tcgctccacc gcttccgtca gtttctttag ctggctctca aacagcggat atcggaacag
     4681 ccgccagccg ttcacttcca ttaaatcaac gcctcatttc ccaaatcctc ctcgggagcg
     4741 gtgtccacat cactcacgag cgcgccgatg cgctccatga gtccggcatc gagcggtgta
     4801 agctgttcgg gagaacgcag catatccgcc gcgagaaagc tcataaacga ggagagaacg
     4861 ggatcttcct cggcgtactt tcttttcggt ttgggatcgg cgaccacaag catgcggccc
     4921 ggtccgatga tgtgggcttc cacttcaccg ctgaattcgg gatggctatg gaacaatgcg
     4981 cgctcgaagc gtaaaccgag agaattgccg gtcttcgctt gcttgccgaa aaacactcca
     5041 gaatccctta cctttgccac ggcacgctct cttctcttgt gccgtacacc agccttcgcc
     5101 tttggcatat cctcatacct cgtaaattca tgttagcacg cttgtactaa caataaaaga
     5161 ggcgaagcac tcgagtttcc tgcggtgagt tggactggcc tggacttccg cttcccgcat
     5221 cgtcaccgaa tctcaattac cctcgcctgg cgtatcgccg cgattgcacg caactcgccg
     5281 aggacatcgg gcggcactgt gtgatcgacg ttgacgatgc cgatggcttc gtcggaattc
     5341 tgcacgcggc ctagcgcgaa gttggcgatg ttgatctcgt gttttccgag cagcgtgccc
     5401 acgcgcccga tgacgccggg gacatcctgg ttgcggaata gcagcaggaa tccggtgagc
     5461 ggcgcgtcga tatcgatgtt gttcacgccg aggatccgca acgcatcgtt cggcccgatc
     5521 atgccgagga cagaaacggt ttcgccgtct ttgcgcaagg ccacgccgag cgaattggtg
     5581 tacttggcgc ggcgggcgct gcgcacttcg accacttcaa tgccgcgcgc t
//
LOCUS       DASZMB010000199         4434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_770281, whole genome shotgun sequence.
ACCESSION   DASZMB010000199 DASZMB010000000
VERSION     DASZMB010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770281"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VG272_09145"
     CDS             complement(<1..541)
                     /locus_tag="VG272_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWF13885.1"
                     /translation="MSFSTPTPLNPAHFLDRAAKIFSQRTAIVDSLRSYTYAQFGERS
                     HRLAGLLKNAGAKPGDRIGALCTNSHVMLEMHNGVPMAGCALVPINIRLSTQEIAYIL
                     ENSGARILIATHEFAEMARRLSASTDVRPIVAGDETSEYETLLQCAEPVPTEPMDEMS
                     LLAINYTSGSTGRPKGVMYT"
     gene            complement(698..1465)
                     /locus_tag="VG272_09150"
     CDS             complement(698..1465)
                     /locus_tag="VG272_09150"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWF13886.1"
                     /translation="MQHFGAFVVGVIIGFLGGLFGKGGSAIATPLLAIVGFPGFIAVA
                     SPLPATIPGTLLASAQYWKHRLVDWQVVSWSTGVGIPATILGSLVTKFIGARFLLILT
                     AIFVLGFGLSFLISPTEKTSVTSLPTPVDQEKVVFSRMRLALIAAGVGFISGLLANAG
                     GFLLAPAYIRLLKLPIKKSFACSLAVSAALAIPGTIIHAYLGHISWTVTGLVALGSIP
                     CSMIGARIAIRSRSTHLARMYGLFLTALGILSFFHLG"
     gene            complement(1580..3388)
                     /locus_tag="VG272_09155"
     CDS             complement(1580..3388)
                     /locus_tag="VG272_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWF13887.1"
                     /translation="MKSFHWIFAALLVVLWPACSSNAQTTKGAVDYEDHCATCHGNPK
                     STTPAPDVLSLWKLTAEELYAAQGKGAHSSMQGATDDDWREVAYYLGRRRVGITKVTD
                     AKLMPNQCPGNSPLGDLSAKPMWNGWGNSIANTRFQSAKEAGLTAAQVPNLKLKWAFG
                     FPGAEEVYGQPTVAGGRVFLGVDTGAVYSIDAETGCVYWSYQADAGVRTAVTINTLKG
                     KGPKYGVYFGDIRANMYMLDASTGKEIWKVKVDDHPVAKITGAPTLYEDRLYVPVSSQ
                     EERSAGWSTVYPCCTFRGSVVALDASNGHTVWKTYIVKDPPKPMEKTASGVQEYGPNG
                     GAVWNSPTVDPKNHAIYIGTGDSYTKPVSKNTDAIVALDMKTGKIMWSVQDTENDAWL
                     SGCGGEIKSENCLPPNEIGPDYDFGASPILVSLGNGHRMLVAGQKSGMVWGHDPDREG
                     TVVWKTPLVKKLALGMITFGGASDDQNSYFGLRSGGIAAVRNDTGEQKWFTPIAPTPG
                     PGSKGENAAITAIPGAIFSGSWDGIMRAFSTDDGHLLWEYNMLHNYDTVNHVAAKGGS
                     MGATGPTVAGGMLFVGSGYVFTGGIPGNALLVFAAQ"
     gene            3644..4093
                     /locus_tag="VG272_09160"
     CDS             3644..4093
                     /locus_tag="VG272_09160"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWF13888.1"
                     /translation="MNLNDTIGLLLKQKGQSVWHISPEACVYDAIELMADKYVGALLV
                     VSDNALVGVLSERDYARKVILEGKSSKETQVKEIMSTPPVFVTPEQTVEDGMRIMTEK
                     HIRHLPVVEDGVILGVVSIGDMVKWIISAQEHTISQLNSYISGAYPG"
     gene            complement(4202..>4434)
                     /locus_tag="VG272_09165"
     CDS             complement(4202..>4434)
                     /locus_tag="VG272_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13889.1"
                     /translation="VTAKAQAKAWQTVARSVAIHASKQSVAASKPFQASLKSDLESDS
                     ADWEQRIQVRLMELDVDPGNDADQAAITKSRD"
BASE COUNT          972 a         1220 c         1242 g         1000 t
ORIGIN      
        1 gcgtgtacat cacgcctttt gggcggccag tgctgccact cgtatagttg atggcaagca
       61 gggacatttc gtccatcggt tcggtcggaa ccggctccgc gcattgcagc aatgtttcat
      121 actcgctcgt ctcgtcgccc gcaacaatgg gccgcacgtc cgttgaagca gagagtctcc
      181 gcgccatttc cgcgaactca tgtgtggcga ttaagatgcg tgcgccggaa ttttcgagga
      241 tgtaagcaat ctcctgcgtc gaaagacgga tattaatggg gacgagcgcg cagcccgcca
      301 tggggacgcc gttgtgcatt tcgagcatca cgtggctgtt ggtgcagagg gcaccgatgc
      361 ggtcgcctgg cttcgcccct gcatttttca gcaatcctgc gaggcggtga gacctttcgc
      421 caaactgtgc gtatgtgtag ctgcggaggc tgtccacgat ggccgttcgc tgcgaaaata
      481 tcttcgctgc gcgatccaga aaatgtgccg ggtttaaagg tgtaggcgta ctgaaagaca
      541 tttcctgctc cgattttggt gcgctttaaa ttatcactaa cgacgacgcg ccgggagatt
      601 ttccagtagg acaggcttga gcctgtctgc ttttgacttc ttgttgcgtc accacaaatt
      661 cagaagaaga caagctcaag cctgtcctac tgaaaaatca ccctaaatga aaaaaggaca
      721 ggatgcccag cgcagtgaga aacagtccgt acatgcgcgc gaggtgagtc gatcgcgagc
      781 gaatggctat gcgcgcgccg atcatcgaac agggaatcga accgagcgcc acaagccccg
      841 tcacagtcca ggaaatgtgc ccgaggtacg cgtggatgat tgtgccggga atggcgagtg
      901 cagcggagac ggccagcgag caggcgaaag atttcttaat gggcagcttc aagagccgaa
      961 tgtaggcagg cgcgagcaga aatccccccg cgttcgcgag caggccggaa ataaatccca
     1021 cgccggcggc aatcaacgcg agcctcattc gtgaaaaaac cactttctct tgatcgacag
     1081 gggtcggaag agaggtgacg gatgttttct cggttggcga gataagaaat gaaagtccaa
     1141 acccaagcac aaaaattgcc gtgagaatga gcaagaatcg cgcgcctatg aacttggtga
     1201 ccagggagcc gagaattgtg gcaggaattc cgactccggt actccacgag acaacctgcc
     1261 aatcgacgag ccgatgcttc caatattgag cggaggcaag taatgttccg ggaatcgtgg
     1321 ccggtagtgg agacgcgaca gcgataaaac cgggaaaacc aacgatggcc aacagaggcg
     1381 tggcgatggc gctgccgcct ttcccaaata aaccgccaag aaaaccgatg atgactccca
     1441 ccacgaaggc cccgaaatgc tgcaagtttc cgcctttcgc atgaatgcaa ggccgacagt
     1501 agcgcgggct tcagccttcg gggtttagtc caggaaggac caaattccgc aggctgaagc
     1561 ccgctctagt gaaaccaatc tattgcgcgg caaaaaccag gagtgcgttg ccggggatgc
     1621 ctccggtaaa cacgtagccg gatccgacga agagcatgcc gcctgcgacg gtcggtccgg
     1681 tggcgcccat cgagccaccc ttggccgcta cgtggttaac tgtatcgtaa ttatggagca
     1741 tattgtattc ccatagtaaa tgtccgtcgt ccgtggagaa ggcgcgcatg atgccgtccc
     1801 agcttccgga aaagatggct ccaggaatcg cggtaatggc cgcattttca cctttggatc
     1861 cggggcccgg ggtcggcgcg atgggcgtaa accacttctg ttcgccggta tcgttgcgca
     1921 ccgccgcgat gccgccgctg cgtaatccga aataagaatt ttgatcgtcc gaggcgcccc
     1981 cgaaggtgat catgccgagg gccaacttct ttacaagcgg cgtcttccac acaaccgttc
     2041 cttcgcggtc tggatcgtgg ccccagacca tcccgctctt ttgtccggcc acgagcatcc
     2101 tatggccgtt acctaaggag accagaatcg gcgatgcgcc aaaatcatag tccggtccaa
     2161 tttcgttggg cggcaaacaa ttttccgatt taatctcacc gccgcatccg gaaagccatg
     2221 catcgttttc cgtgtcttgc acagaccaca tgatttttcc cgtcttcatg tcgagcgcga
     2281 ctatggcgtc ggtgtttttt gagactggct tcgtgtagga atcgccggta ccaatgtaaa
     2341 tggcatgatt ctttggatcg accgttggcg aattccagac cgcgccgccg tttggaccgt
     2401 attcctgcac gccgctggca gttttttcca tcggcttggg cggatctttc acgatgtacg
     2461 ttttccaaac agtgtggccg ttgctcgcgt cgagagcgac gacactgccg cggaacgtgc
     2521 agcacgggta tacggtgctc caaccagccg agcgctcttc ctgcgaagac actggcacgt
     2581 agagccggtc ctcatataac gtcggcgctc ccgtgatttt cgcgacggga tgatcgtcca
     2641 ctttcacttt ccagatttct ttgcccgtag acgcatccag catgtacatg ttcgcgcgaa
     2701 tgtcgccgaa gtaaacgccg tatttgggcc ctttaccctt cagcgtgttg atggtgacag
     2761 ccgttcgaac gccggcatcc gcctggtagg accaatagac acagcccgtt tccgcgtcaa
     2821 tggaatacac cgcgccggta tcgactccta aaaatacgcg gccgccggcg accgtaggct
     2881 ggccataaac ttcttcagcg ccggggaaac cgaacgccca ttttaatttg aggtttggca
     2941 cctgcgccgc ggtcagtccc gcttctttcg ccgactgaaa gcgcgtgttg gcgatactgt
     3001 tgccccatcc gttccacatg ggtttcgcgg ataagtcgcc gaggggagaa tttccgggac
     3061 actgattcgg catgagtttt gcgtccgtca ctttggtgat tccaacgcgc cgccgaccca
     3121 aatagtaagc gacctcgcgc cagtcgtcat ccgttgctcc ctgcattgaa ctatgcgcac
     3181 ccttcccttg cgccgcgtac agttcttccg ccgtcaattt ccacagggat aggacgtcgg
     3241 gtgcaggggt cgtgcttttt ggatttccgt ggcacgtagc gcagtggtct tcgtagtcca
     3301 cggctccttt ggtggtctgc gcgttcgaac tacatgcggg ccagagcacg acgagcaggg
     3361 cggcgaaaat ccaatggaat gatttcatgc gcatgattcc ctcgaatcaa aaaatatttt
     3421 ttgcgatgta actgtctgcg ccttaatata ccgcgctctt gatgctggca caatgcaagc
     3481 caccgctact gcggtttgcg ccgcacaacc gattgatggc gttttttcac ggataattcg
     3541 ccttgacagc gcatagcagc ggacgtatgt tcgcgagtaa ccggcatcaa attggttgtt
     3601 tgatgccccc cggccaaact tccgcagtcc aggaatgaca cctatgaacc tcaacgacac
     3661 gattggattg cttttgaaac agaagggcca aagcgtctgg cacatttccc cggaagcgtg
     3721 tgtgtatgac gcgatcgagt taatggcgga caaatatgtc ggagccttgc tcgtcgtttc
     3781 ggataacgca ctggtcggcg tgctttccga gcgcgactat gcgcggaaag tgatcctgga
     3841 ggggaagtcg tcaaaggaaa cgcaggtcaa ggaaatcatg tcgactccgc ccgtgtttgt
     3901 gacgcccgag caaaccgtgg aagacgggat gaggatcatg acggagaaac atattcgcca
     3961 tctcccagtg gtggaggatg gcgtcatcct gggcgtcgtg tcgattggcg atatggtgaa
     4021 atggatcata tccgcgcaag agcacacgat ttcgcaactg aacagctaca tttctggggc
     4081 atatccggga tagccgaccg tgttcctgcg cagcacaatc gtttcaagac tttttatgca
     4141 aatcctccca ccccccacga actagtggat gtgggaggat ttgcaatttt cagaactgga
     4201 atcagtctcg gctcttggtg atggctgcct gatcggcgtc attgcctgga tcgacgtcca
     4261 actccatcag gcgaacttgt atgcgttgtt cccagtcggc ggaatcactt tccaggtcac
     4321 ttttcaaaga cgcttgaaat ggtttgcttg ctgccacgga ctgtttcgat gcgtgaatcg
     4381 caacgctcct ggctactgtt tgccatgctt tcgcttgcgc tttcgccgtg acgg
//
LOCUS       DASZMB010000200         4285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_196788, whole genome shotgun sequence.
ACCESSION   DASZMB010000200 DASZMB010000000
VERSION     DASZMB010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196788"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1465)
                     /locus_tag="VG272_09170"
     CDS             complement(170..1465)
                     /locus_tag="VG272_09170"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HWF13890.1"
                     /translation="MKKQLFAAILVIFSAANMLVAQSEGPKTGAWSGVIVNGTCTPDE
                     AFAEAAKCTDATTGELFALYDDTIRQVYVLDPQDAAKGHMGESVTVQGSLNGNSLHVD
                     SLKMFAEVGLRAGQKAPDFSARDQFGNTQTLETLRGAKGTVLLFYRSADWCPYCKGQL
                     IQLQAAKKRFEEQGIKLAGVSYDSVEILKFFSARRKIEFPLLSDPDSKIIRMYQVLNA
                     EGLGPNAGMARPGYFFIDPKGNIREKFFEAKYRERLTGNNVLSKLFPELGEEVSDAVD
                     APHLQLHVGQSDRVAVPGNLVTLTAEVQLPPDVHVYAPGTKGYKPIKLVIEPASEFSF
                     KQATYPVAKILYLPAIKEKVPVFEGKFKIQQEVKANSATDFSGALGTDGKQVTLKGTL
                     EYQACDSTICFLPASVPVEWHLQIVPLDRQRAPEDIRHK"
     gene            1596..3221
                     /locus_tag="VG272_09175"
     CDS             1596..3221
                     /locus_tag="VG272_09175"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWF13891.1"
                     /translation="MLSLNNITMRYGARLLFEEVTCSFLEGRRYAITGPNGAGKTTMM
                     KILEGDLEPTTGSVSRPKKLGILRQDQFAFDEFRVIDTVIMGNGPLWKALQERDILYA
                     KPHEELTDKDGMRLGELEGVVGDEGGYTAEADAAVLLDGLGVEAALHERKMSELQGGQ
                     KVRVLLAQALFGNPAALLLDEPTNHLDLDSVHWLQDYLHEYQGVLIVISHDRHFLNSV
                     CTHTADIDYQTVIMYTGGYDEMVLAKTQIRSRIEADNAVREKKIAQLNEFIARFSAGT
                     RSSQVTSRKKEVERIQTSELARSNIARPFIRFDLKIPSGRHPLEFKSVTKSYDGHKVI
                     DKFTANVERGEKIAIIGRNGSGKTTLIKSLIKNATGDIEESDRHFPVDGGHVKWGHEA
                     VVGYFAQDTTDTIAKNTTVIEWLHQFDQKASQQELRAVLGQMLFSGEDAEKSTRALSG
                     GEQARLIFCKLMLQKPNVLVFDEPTNHLDLESINALNIALQKYPGTVLFVTHDHDVID
                     EVATRIWQCRGGHVEDFKGPYEDFLVYAKQKAS"
     gene            complement(3311..3985)
                     /locus_tag="VG272_09180"
     CDS             complement(3311..3985)
                     /locus_tag="VG272_09180"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWF13892.1"
                     /translation="MFIAFPALVFTGVPSIIANASSTIALIPGVLASAGAYRDDFRKS
                     ENFPFLPLLVASVLGGIVGALLLLYTPQSTFDSVIPWLLLMATLLIAFGSRISPLLKR
                     VIHIGPVTVVVIQFFIAIYGGYFGGAIGILMLATWTVFGLKDIHVMNANRTLMGAAAN
                     SIASIMFIVAHKVWWPQTLVMLVFCTIGGYYGAVAAKKVRPSYVRIFVIVISTAITIA
                     FFLRRH"
     gene            3978..4223
                     /locus_tag="VG272_09185"
     CDS             3978..4223
                     /locus_tag="VG272_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13893.1"
                     /translation="MNIPPPATELMTPARRAAILSKRYELMCILNLQIYHFAPETPSR
                     WLEANYYGVFLCFAQNRRPKKTDGLYEDRRLSRVHVV"
BASE COUNT         1020 a         1212 c         1138 g          915 t
ORIGIN      
        1 ttccgaccga agcgattgat gacggccaat gtcacgtagc acagccactc ttggctgtgt
       61 gacgtttgca aaacccgcac aggcaagagt gcctgtgcta ccaaacattc gaccgaacca
      121 cagaataaat ttctttcggg cgtcagtcag agctacaaca cgatgaaagt tacttgtggc
      181 gaatatcttc cggtgcgcgc tgtcgatcga gcggaacaat ctgaagatgc cactcgaccg
      241 gaaccgaagc gggcagaaaa caaatcgtgc tgtcgcaggc ctgatattcc agcgtcccct
      301 tgagcgtgac ctgctttccg tccgtgccta gcgcgccgct gaaatccgtg gccgagttcg
      361 ccttcacttc ctgctggatt ttaaatttac cttcaaatac gggcactttt tctttgatcg
      421 cagggagata caaaattttt gcgacaggat aagtcgcctg cttgaatgaa aattcagacg
      481 ccggttcgat caccagcttg atcggcttgt aacccttcgt cccgggcgca tagacgtgca
      541 cgtccggcgg taactgcact tccgcggtga gcgtgacgag attgcccggc acggccacgc
      601 ggtccgattg ccccacatgc agctgcaaat gaggcgcatc caccgcgtca ctcacttctt
      661 cgcccagctc cgggaaaagt ttcgacagca cattgtttcc cgtgagccgc tctcgatatt
      721 tcgcttcaaa aaatttctca cggatatttc cttttggatc gatgaaaaaa tatcccggcc
      781 gcgccatgcc cgcattcggc cccaagccct ccgcgtttag cacctggtac atgcgaataa
      841 tttttgaatc tgggtcggag agaagtggga attcgatctt gcgccgggcg gagaaaaatt
      901 tcaggatttc cacgctgtca tagctgacac ccgcgagctt gattccttgc tcctcaaatc
      961 gcttttttgc ggcctgcagc tgaatgagct gccctttgca gtacgggcac caatccgcgg
     1021 agcggtaaaa caagagcacc gtgcccttcg cacccctcaa ggtctccagc gtttgcgtat
     1081 tgccaaactg gtcgcgcgct gaaaaatcag gagctttctg ccccgcgcgt aatcccacct
     1141 cggcaaacat tttcagcgaa tccacgtgaa gggaattgcc attcaagctg ccctgaacgg
     1201 ttacgctctc ccccatatgc cctttggccg cgtcctgcgg atcgaggacg taaacttggc
     1261 ggatcgtatc gtcgtatagc gcaaacagct cgcccgtcgt cgcgtcggta cacttcgcgg
     1321 cttcggcgaa ggcttcgtcc ggcgtacatg ttccatttac gatcaccccg gaccatgcac
     1381 ccgtctttgg accttcactt tgggctacca gcatgttcgc agcggaaaaa atcaccaaaa
     1441 tagccgcaaa tagttgcttt ttcatatttg tatcatcccg tcacttcgat taaacttaca
     1501 ctccggactt caagcgcctc cgctttgatt cagaatacgc cccgcggccc tgatcaacaa
     1561 gcacgcttgc cgatttcacg ctattatgga gagatatgct ttcgttaaat aacatcacta
     1621 tgcgctatgg cgcaaggctg ctattcgagg aggtcacctg ctcgttcctc gaagggcgcc
     1681 gctacgcgat tacgggcccc aatggcgccg gtaagaccac catgatgaag attctggaag
     1741 gcgatctgga gccgacgaca ggctccgttt cgcggccgaa aaaactggga attttgcgac
     1801 aggatcaatt tgcttttgac gaatttcgcg tgatcgacac tgtcatcatg ggcaatggac
     1861 cgttgtggaa ggctttgcag gaacgcgaca ttctgtacgc caagccgcat gaagagttaa
     1921 cggacaagga cggcatgcgc ctcggcgaac ttgagggcgt ggtgggcgac gagggcggat
     1981 acacggcgga agcggatgcc gcggtgctgc tggacggcct gggcgtggaa gcggcgctgc
     2041 atgagcgcaa gatgagcgag ttgcaaggtg gacaaaaggt ccgcgtgctg ttggcgcagg
     2101 cgctcttcgg caatcccgcc gcacttctgc tggacgagcc gacgaaccac ttggacctcg
     2161 attccgtgca ctggctgcag gattatcttc acgagtatca gggcgtgctg atcgtcatct
     2221 cgcacgaccg ccatttcctg aacagtgttt gcacgcacac cgccgacatc gactatcaaa
     2281 ctgtgatcat gtatacgggt ggctacgacg aaatggtgtt ggccaagaca cagattcgtt
     2341 cgcgcatcga agccgacaac gcggtacgcg aaaagaaaat tgcgcagtta aatgaattca
     2401 tcgcgcgctt ttccgccggt acgcgatctt cgcaggttac ttcgcgaaaa aaagaagtgg
     2461 agcgcattca gacttccgag ctggcgcggt cgaacattgc gcgcccgttc attcgctttg
     2521 acctgaaaat tccatccggg cggcatcctc tggaattcaa gagcgtcaca aaatcctacg
     2581 atggccacaa agtgattgat aaattcacgg ccaacgtcga gcgcggcgaa aaaatcgcca
     2641 tcatcggccg caacggctcc ggtaagacca cattaatcaa atcgctgatt aaaaatgcga
     2701 cgggagatat cgaagaatcg gaccgccact ttccggtcga cggcggccac gtcaagtggg
     2761 gccacgaagc ggtggtcggt tatttcgcgc aggacaccac ggacaccatc gccaaaaata
     2821 caaccgttat cgagtggctg caccagttcg atcaaaaagc gtcgcagcag gaattgcgcg
     2881 ccgtacttgg acagatgctc ttcagcggcg aggacgccga gaaatccact cgcgcgctgt
     2941 ccggaggcga acaggcccgc ctgatcttct gcaagctgat gctgcagaag ccgaacgtgc
     3001 tggttttcga cgaacccacg aaccacctcg acctcgaatc gatcaacgcg ctgaacattg
     3061 cgctgcaaaa atatcccggc accgtcctgt tcgtcacgca cgaccatgat gtgatcgacg
     3121 aagttgcgac gcgaatctgg cagtgcaggg gcggccacgt cgaggatttc aagggaccgt
     3181 acgaagattt cctggtctac gcgaagcaga aagcctcgta gttggttttc agtagcatag
     3241 gcttcagcct gtgggacttt ggtccttgca aggtctagat tccacaggct gaagcccgtg
     3301 ctagttaaac ctagtgccgg cgcaggaaga acgcgattgt gatggctgtg ctgatcacga
     3361 tcacgaaaat gcgcacatag ctggggcgaa ctttttttgc cgccaccgct ccgtaatacc
     3421 cgccgatcgt gcagaaaacg agcattacca gcgtctgcgg ccaccacact ttgtgagcca
     3481 cgataaacat aatggacgcg atgctgttcg ccgcggcgcc catcaaagtc cggttcgcgt
     3541 tcatcacgtg aatatccttg aggccgaaga ccgtccacgt ggcgagcatc aggatcccaa
     3601 tggccccgcc gaaatagccg ccgtaaatcg cgataaaaaa ttgaattacc acaacggtca
     3661 cgggacctat gtgaatgacg cgcttcagca ggggtgaaat tcgcgagccg aatgcgatca
     3721 gcagggtcgc catgagcagc aaccatggaa ttacagagtc aaacgtgctc tgtggcgtgt
     3781 aaagcaaaag caaggcgcca acaattcccc ccaatacgct cgcaacgagc agaggcaaaa
     3841 aagggaaatt ctccgatttg cggaaatcat cccggtaggc ccctgcgctc gccaagactc
     3901 cgggaattag cgcgatggta ctggaggcat tcgcgataat cgaaggcacc cccgtaaata
     3961 ccagcgcggg gaacgcgatg aacatacctc ctccagcgac ggaattgatg acccccgcca
     4021 gaagagcggc aatactcagc aagagatatg aattgatgtg catattgaat cttcaaattt
     4081 atcattttgc gccggaaacc cctagccgct ggttggaagc gaattattac ggcgtatttc
     4141 tttgctttgc acaaaatcgc cggcccaaaa aaacggacgg cctctacgag gaccggcggc
     4201 tttcgcgcgt tcacgttgtg tagtgcgctc ttcgtcccgc agttttttgg gacccagagc
     4261 gcagccttgg cgctcctatg acccg
//
LOCUS       DASZMB010000201        10171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1606930, whole genome shotgun sequence.
ACCESSION   DASZMB010000201 DASZMB010000000
VERSION     DASZMB010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 10171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10171
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606930"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VG272_09190"
     CDS             complement(<1..872)
                     /locus_tag="VG272_09190"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HWF13894.1"
                     /translation="MKISRRIFLKGGGISMIGLSTIPAFLQRAVAATAMPNKKKLIVL
                     FQRGAADGLNTVVPFGEENYYRLRPSIAIPQPRAGVEGTAVDLDGFFGLHPSLAPLGP
                     LFHNGQLAIVHAAGSPDATRSHFDAQDFMESGTPGVKSTDDGWLNRVVGNMAEQDATP
                     FRAVAMGPNLPLTLRGAAPAIALADVKQFRVMSQSTAVQGGFEALYAQTVDKVLRGTG
                     TETFEAIDMLRKADPSRFQPENGAEYPKSRIGQTLQQVAQLMKADIGMEVGFVDTGGW
                     DNHVNEGGAQGQMA"
     gene            complement(1085..3097)
                     /locus_tag="VG272_09195"
     CDS             complement(1085..3097)
                     /locus_tag="VG272_09195"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HWF13895.1"
                     /translation="MQKRIARTDTTRPGRHAPLSVMIAIFLLAAATFGAPLLLATKDK
                     RDAETKSQESSRAKKGLPTEGLTDDEAILQALNRLGFGPRPGDIERLKEIGLQKWVDG
                     QLHPDSIDDSALNARLERFPTLKMSTAKLFDEFPEPRVAARREGITVEEYRKEQQEHL
                     RAAVQGSPQGDASLAPAQTDIQKADALHMPDFDAVDNDLNANPAKGKGTGKGQGGFGN
                     RMVNYEQIKLPQRVVAELSMAKMTRAVYSERQLQEMMVDFWYNHFNVFAAKGADRWLI
                     TEYERDAIRPNAMGKFRDLLGATAKSPAMLFFLDNWLSADPVAWQKMQQQMLARRQMR
                     GGAFGTPRFPHQSPMPADDPNNPNTNANAKQKQERGLNENYGRELMELHTLGVDGGYT
                     QNDVVNVAKAFTGWTIKQPRREAEFFFDDRLHDNSMKTVLGHQIHAGGMHDGEQVLDI
                     LARDPHTAHHISYEIAQRFVSDNPPAALVDRMAQTFLKSDGDIREVLHTMIYSPEFWA
                     KDSIRTKIKTPFELVASATRAVGGDTEIPLMLVQWTSRIGQPLYQCEPPTGYSDQADA
                     WVNTGALLTRMNFSLALTSNKIRGVTVNADTLFGNDTAANPHATLDHAILLLLGGAAT
                     QQTRDTLEKQLDDPQILQANLDDPVKSVNVPVIAGLVLGSPEFQRR"
     gene            complement(3265..4095)
                     /locus_tag="VG272_09200"
     CDS             complement(3265..4095)
                     /locus_tag="VG272_09200"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HWF13896.1"
                     /translation="MNPEMTSTFLRRFYRAVLRVVPSCVTLSQAVAFNMFLAFSPMIL
                     VVLGTVAGSAAFRQALQGIIERLRIVLPPGTLTILYNFLGRHGTQSWQWVLLGLGGTL
                     LAGTQMMKLMMEGFRRIYGDEEELSVVGIHLRALLLLTATILPSIVIVNLVVFGRQVR
                     NRMLHISSMPVLIRLTWSGLYIITSLLFALAVLTVIYRVGRPRHHGLTAHLPGAEVAT
                     ILWWVVSISLGFYLRTVPYSLIYGGLAVTIGLMLWMQLTATILFIGAAYNAERLESSK
                     "
     gene            4356..5210
                     /locus_tag="VG272_09205"
     CDS             4356..5210
                     /locus_tag="VG272_09205"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HWF13897.1"
                     /translation="MQLNRLLKAGFGRRGFLSGSSLLLAGSLLGTAEEMSAGHEPAPR
                     LPEAVPQDKQDKQALLEDLVTANHILQDQGVVDGYGHVSVRNPANPNHFFLSRWLAPD
                     LVTVADIMELDFDCVPAGGDNRRMYSERWIHAEIYRARPEVKSVVHTHAPTVVLMGTI
                     NEPLLPIYHMAGFIGSGLPSFDIRKTFGNTNMLITDAAKGKALAQALGQAPAALMRGH
                     GGVTVGTNVMHSVGRSVYLKINSEMHLQAGSRKVETLNLEEAHLAEQGNSDFPKDWDL
                     WKRKIMKS"
     gene            5349..6251
                     /locus_tag="VG272_09210"
     CDS             5349..6251
                     /locus_tag="VG272_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HWF13898.1"
                     /translation="MARIVRSGLIQAHCEWSPTNLSLAKIKENMIAKHEKLIAAAAKK
                     KVQILGLQELFYGPYFCAEQQQKWYELTESVPDGPTVVRMQKLAKKYGMAMVVPVYEK
                     EMTGLYFNTAAVFDADGKYLGKYRKHHIPHCHPGFWEKFYFTPGNAGYPVFETKFAKV
                     GVYICYDRHFPEGARCLGLNGAEIVFNPSATVAGLSEYLWELEQPAHAAANGYFMAAS
                     NRVGVEKPWQIGEFYGKSYFCNPRGKIFAQASRDKDELLVADLNLDEIQEVRDVWQFY
                     RDRRPETYGEITRTLTSRGSQAAD"
     gene            6506..7819
                     /locus_tag="VG272_09215"
     CDS             6506..7819
                     /locus_tag="VG272_09215"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWF13899.1"
                     /translation="MTTQPKQTTRSEEVVRKHKEFLWPAVTNFYKEPLVADHGSMQHV
                     WDVEGRKYLDFFGGILTVSVGHANPAITGPVKAQIDKFQHLSTLYPNEAIVNLAEKVS
                     QITPGALKSSFFTSSGTEANEAAILLARMATGSFDIVALRHAYAGGSSLTKSVTAQAP
                     WRKSGIISLGISHAINPYCYRCPLGLKYPDCEVACAKDVENLIQTGTSGQIAAFMAEP
                     IQGVGGFITPPKEYFKIVFNAVKKYGGLFISDEVQTGFGRTGKKWFGIEHWEVTPDIL
                     TCAKGMANGAPIGATITTKELAASYQGLTISTFGGNPVTSVAAKATIEFIEEEKLLEN
                     SETVGNYFRGKLDELQQKHAWIGDVRGKGLMLGMELVKDRTTKEPAPEATAQLLERTR
                     EHGLLIGKGGLYGNVIRLSPMLNIGKGDVDEAVRILDISFTDLPH"
     gene            8044..9975
                     /locus_tag="VG272_09220"
     CDS             8044..9975
                     /locus_tag="VG272_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HWF13900.1"
                     /translation="MVSTKRGSGPLAQLVQPLYPMKKKKLGELLRDRGMISSPDLVSA
                     IAAQQGKAIHLGEIMLDRGFVSKEDLGAALEEVWQIPYLDCATITPEKDALNLVTQAI
                     AERLCVLPVRTEQRRLVVAMAAPQNLAMLDELRFKTGMEISPRLSFRKELELAIRAHY
                     GARTERVSSTEHSDSQKMKFEEVEFFSTSSRQANQEAIQEVQADLRQKKTPAVRLVSE
                     AIQIAMAKRASDIHIEPRTADTVVRMRVDGVLHDYQFIPRTLQNSLISRIKILSDMDI
                     SERRNPQDGRFMVSVGGRQVDLRVSTLPTQYGEKVVIRLLDTSAPLTSYADLGMPGDV
                     AEGLNELLAGPRGMILVTGPTGSGKSTTLYSSLNKLRKDSVNIVTVEDPVEYVLPGIN
                     QVHVNTKAGLTFVSCLRSILRQDPNIIMVGEIRDKETAEIAMKASQTGHVVLSTLHTN
                     DSVSAVNRLLDLGIPGYLISASVSGILAQRLVRKLCPCHSHQPATPAFKARMVQLGSS
                     EAPANMAVANGCGKCNHTGYAGRLGIYELLRIDDSIRPIIRANGSVEQIRAVARMNGM
                     RLMQEDALEKVRTGVTTLEEVLRVVPMENATHSECLQCKSRILPTFNYCPACGAKCMA
                     APAAPNRSSNRELVPEGIL"
BASE COUNT         2350 a         2679 c         2893 g         2249 t
ORIGIN      
        1 ttggccatct ggccttgcgc gccgccttcg ttcacgtgat tgtcccagcc gcccgtgtcg
       61 acgaaaccaa cctccatgcc gatgtccgcc ttcatgagtt gcgccacttg ctggagcgtt
      121 tgcccgatgc gacttttggg atactccgcg ccgttttcag gctgaaagcg cgaagggtcc
      181 gccttgcgca gcatgtcaat ggcttcaaaa gtttccgtgc ctgtgccgcg cagaactttg
      241 tcgacggttt gcgcgtagag cgcttcaaag cctccctgca cggcggtgga ctggctcatc
      301 acgcgaaatt gttttacgtc ggccagtgcg atcgctggcg cggcgcctcg caaagtgagc
      361 ggcagattcg ggcccatggc tacggcacgg aatggggtgg cgtcttgctc ggccatattg
      421 cctaccacac ggttgagcca accgtcatcc gtggatttca cgcctggcgt gccggattcc
      481 atgaagtcct gcgcatcgaa atgggagcgc gtggcgtcag gagatccagc ggcgtgaacg
      541 atggcgagct gcccgttatg aaaaagtggg ccgaggggcg cgaggctcgg atgcaaaccg
      601 aagaaaccat caaggtcgac ggctgtgcct tcgactccgg ctcgaggttg cggaatcgcg
      661 atgctggggc ggagtcgata atagttttct tcgccgaagg gcacgacggt gttcaggccg
      721 tcagcagcgc cgcgttggaa gagcacgatt aattttttct tattgggcat ggctgtcgcg
      781 gcgaccgcgc gttgcaggaa cgcagggatc gtgctcaggc cgatcatgga gatgccgccg
      841 cctttaagaa aaatacgtcg cgaaattttc atcgtcgtgc tcctggtggt gcttgtgctg
      901 atcttgctca cacttgtctg atactcgcgc ctttcattct tccgcttttt tatcttgcct
      961 tctgtagctc gctcctcaga agcgaggctt ttcttgctgt tgaaaggcca tttcccctca
     1021 gaagtttcct tgccatcggc gcaaaagcct cgcctctgaa gaggcgagct acaaggaacg
     1081 gcgatcagcg gcgctgaaac tcgggtgaac ctaaaaccaa acctgcgata accggaacgt
     1141 tcacggattt tacgggatcg tccaggttgg cctgcaaaat ttgcggatcg tccaattgtt
     1201 tttccaatgt atcgcgcgtc tgctgcgttg cggcgccccc tagcaacagt aggatggcat
     1261 gatcgagtgt ggcatgcgga tttgcagccg tgtcatttcc gaataatgtg tcggcattca
     1321 ccgtgacgcc acgaatttta ttggatgtga gcgccagcga gaaattcatt cgcgtgagaa
     1381 gggcgccggt gttcacccat gcgtcggctt gatcggaata gccggtcgga ggctcgcatt
     1441 gatagagcgg ctggccaatg cggctggtcc actgcacaag catgagtgga atttccgtgt
     1501 cgccgcccac ggcacgcgtc gcggacgcga ccagttcaaa cggagttttg attttcgttc
     1561 gaatgctgtc cttggcccag aattccggcg agtaaatcat ggtgtgcagg acctcgcgga
     1621 tatcgccgtc ggatttcaag aaagtttgcg ccatgcggtc gacgagcgca gcaggcggat
     1681 tgtccgagac gaagcgctgc gcaatctcat acgaaatgtg atgcgccgtg tgcgggtcgc
     1741 gcgcaaggat gtccagcacc tgttcgccgt cgtgcatacc tcccgcgtgg atctgatggc
     1801 ccagcacggt tttcatgctg ttgtcatgca ggcggtcatc aaagaaaaat tcggcttcac
     1861 gccgcggctg tttaatcgtc cagcccgtga aagctttcgc cacgttgact acgtcattct
     1921 gcgtgtaacc gccatcgacg ccgagagtgt gcaactccat cagttcgcgt ccgtaatttt
     1981 cgttcaggcc gcgctcctgt ttttgcttgg cattggcgtt ggtattcgga ttattcggat
     2041 cgtccgcggg catggggctc tgatgtggga atcgcggcgt gccaaacgcc ccgccgcgca
     2101 tctggcgcct cgccagcatt tgctgttgca ttttttgcca ggctacggga tcggcggaga
     2161 gccaattgtc caggaaaaac agcattgcag gactcttggc cgttgcgccg agcaggtccc
     2221 gaaattttcc catcgcgttg ggacgaatcg catcgcgttc gtattccgtg atgagccagc
     2281 ggtccgcgcc ctttgcggcg aacacattaa agtggttgta ccagaaatcc accatcattt
     2341 cctgcagctg gcgctcgctg taaacggcgc gcgtcatttt cgccatggat aattccgcaa
     2401 cgactcgctg cggcaatttg atctgctcgt agttcaccat gcgattgccg aatccgccct
     2461 ggccttttcc cgtgcctttt cctttcgcgg gatttgcatt cagatcatta tccacggcat
     2521 cgaaatcggg catgtgcagc gcgtcagcct tctgtatatc ggtttgcgca ggagccaacg
     2581 aagcgtcgcc ctgcggagaa ccttgcacgg ccgcacgcaa gtgctcctgc tgctccttgc
     2641 ggtattcctc cacggtaatt ccttcgcggc gcgcggcgac tctcggttcc ggaaattcgt
     2701 cgaacaattt tgcggtagac atttttaacg tggggaaacg ctcaagcctg gcattgagcg
     2761 ccgagtcatc aatcgaatcg ggatgcagtt ggccatcgac ccatttctgc aagcctattt
     2821 ctttcaggcg ttcgatgtcg ccagggcgtg gcccaaaacc gagacgattg agcgcctgca
     2881 gaattgcttc gtcatcagtt aagccttccg tgggcaaacc ctttttcgct cttgacgatt
     2941 cctgtgattt ggtttcggcg tcccttttgt cttttgtcgc cagaagtaac ggcgcgccga
     3001 acgtggccgc agctagaaga aatatggcga tcatgacgga tagcggagcg tggcgtcctg
     3061 ggcgagtggt gtcggtgcga gcaattcttt tctgcattgt taggctctcc ccctgcggga
     3121 gtctgcacgg ccagaactgc gagttggcgg tatctaactc gcgcactcag tctaacccgg
     3181 cgaaaatgaa aaagttgcga gggggaaacc ggaggtgccc agacagtatg cggcgtggac
     3241 agtcatcgga acgatggtcg aaaactactt tgaggattcg agacgttcag cgttgtaagc
     3301 ggcgccgata aaaagaatag tggcggtcaa ctgcatccat agcattaggc cgatcgtgac
     3361 agctaagccg ccgtaaatca ggctgtaagg aaccgtccgc agatagaatc ctagcgagat
     3421 gctgaccacc caccagagaa ttgtggcgac ttccgcaccg ggaaggtgag ccgtcaatcc
     3481 gtgatgacgg ggacggccaa ctcgatagat aaccgttagt accgctaatg caaatagaag
     3541 cgaagtgatg atgtaaaggc cgctccatgt gagacggatc aacacgggca tcgaggaaat
     3601 gtgcagcata cgattgcgta cttgccttcc aaaaacgacg agattcacaa ttacgatgga
     3661 aggaagaatc gtggcggtca gtaaaagaag cgcacgaaga tgaatgccga caacgctcaa
     3721 ttcttcctcg tcgccgtaaa tgcgccggaa tccttccatc atgagcttca tcatttgcgt
     3781 gcctgccaac aaagttccgc caagtcctaa cagaacccat tgccaggatt gggtcccatg
     3841 gcgaccgaga aaattataca gaatagtgag cgtgccggga ggaagcacga tgcgcaggcg
     3901 ttcaataatt ccctgcaggg cttgccgaaa cgcagccgat ccggctaccg tgccaaggac
     3961 cacgagaatc atcggagaaa atgcgagaaa catgttgaag gcgacggctt gcgataacgt
     4021 aacgcaactg ggaacaacgc gaagaacagc ccgatagaaa cgccgcaaaa atgttgaagt
     4081 catttctggg ttcaatgttt ctcccgtgcg gtacgtcgtc agaatagctc actttccacg
     4141 ttttgggcat acactgcccc agggatttca gccgcgacca tactaagacg ccttccgctg
     4201 cagtccacca ttgacacgac cgagtgaaac agtgtatccg atgtgctgcc gaatattccc
     4261 aatcgatcac gtttaggatc gtagtttgtg cgcgtagata gattaagggg cgagtttgtg
     4321 cgaacgcggc ccactcagtg tccaaggaga tttccatgca actgaatcgt ttattgaaag
     4381 ccgggtttgg tcgcaggggc tttctttccg gctcgagcct gctgctggct ggatctttgc
     4441 tcgggacagc cgaagaaatg agcgccgggc acgaaccagc accacgtctg ccggaagctg
     4501 taccccaaga caagcaggac aaacaggcgc tgctcgaaga cctggttacc gcgaatcaca
     4561 ttctgcaaga tcagggtgtg gtggacgggt acggccacgt gagcgtccgc aatccagcaa
     4621 atccaaatca ttttttcctg tcgcgctggc tggcgccgga tctggttacg gttgcggaca
     4681 ttatggaatt ggacttcgac tgcgtaccgg cgggcggcga caaccgaaga atgtatagcg
     4741 aacgctggat ccacgcggaa atctatcgcg cgaggcctga agtgaagtcc gtggtgcata
     4801 cgcatgcgcc aaccgttgtg ttgatgggaa cgatcaatga gccgctgctg cccatttatc
     4861 acatggctgg atttatcggt tcaggtcttc cgtctttcga tattcggaag acattcggga
     4921 atacaaatat gctgattacg gacgcggcca agggaaaagc actggctcaa gcgctcgggc
     4981 aggctcccgc ggcgctgatg cgtggacacg gcggtgtgac cgtcggaacc aacgtaatgc
     5041 actctgttgg gcgcagcgtt tatttgaaga ttaactcgga gatgcatctg caggcgggga
     5101 gcagaaaagt ggagacgctc aacctggaag aggcgcatct ggccgagcag ggaaattcgg
     5161 attttcccaa ggattgggat ctttggaaac gcaagattat gaagagctag gcggttggta
     5221 gcacgcggct ttagcctgcg ggtttagagt ttgcaagcaa ccaaattccg caggctgcag
     5281 cctgcgccgc ttgccgcgct gcgccgcagc ggagtacact cgctgcttaa cttccgcgga
     5341 ggtgcagcat ggctcgaatc gtgcgttccg gtctgataca ggcgcattgc gaatggtctc
     5401 ctaccaatct ttccctggca aaaattaaag aaaacatgat cgcgaagcac gagaaattga
     5461 tcgccgccgc ggcgaagaag aaagtgcaga ttctcggcct gcaggagctt ttctacggcc
     5521 cctacttctg cgcggagcag cagcagaaat ggtatgagct gacggagtct gtgccggacg
     5581 gcccgaccgt ggtgcgcatg caaaaactcg cgaagaaata cggcatggcc atggtcgtcc
     5641 cggtctatga aaaggaaatg accggactat atttcaacac cgcggccgtt tttgacgcgg
     5701 acggaaaata tcttggcaaa tatcgcaagc atcatattcc ccactgccat cccggattct
     5761 gggaaaagtt ctatttcacg ccgggcaatg cgggctaccc cgtgtttgaa acaaaatttg
     5821 caaaagtggg tgtgtatatc tgctacgacc gccactttcc tgagggcgca cgatgcctgg
     5881 ggctgaatgg cgcggaaatc gttttcaatc cgtcggccac tgttgcgggc ctgtcggaat
     5941 atttgtggga gcttgagcaa cccgcgcatg ccgctgcaaa tggatatttt atggccgcgt
     6001 caaaccgcgt gggtgtcgaa aaaccatggc agatcggcga attttacggc aaaagttatt
     6061 tctgcaatcc gcgcggaaaa attttcgcgc aggccagccg cgacaaggat gaattactgg
     6121 tagccgatct gaatttagac gaaattcagg aagtccgcga cgtgtggcag ttctaccgcg
     6181 atcgccgccc ggaaacgtat ggggaaatca cgcgtaccct gacgtcgcgt ggttcgcagg
     6241 cggcggacta gaggcttgaa aacacgcatg cacacggatt tacacggata agagcaaggc
     6301 tccgtaactc gatagaattc tacggaggtc gacggtagat ttttcagggg ctaataccct
     6361 atttttttta gcgtacctga aagccgcggc tgactctgcg acccacgaag actgtttgca
     6421 attcgcggtg atttaatttg aagaaacgtg cattttgctt tatccgtgtt gatctgtgtg
     6481 catccgtggt tccaaagagg tttcgatgac aactcagccc aagcagacga cgcgcagcga
     6541 agaagtagtt cgcaagcaca aggaatttct gtggccagcc gtcacgaatt tttacaaaga
     6601 gccgctggtg gcagaccacg gctcaatgca gcacgtatgg gacgtggaag ggcgcaaata
     6661 cctggatttc ttcggcggga ttctgacggt ttccgtcgga catgcgaatc ccgcgattac
     6721 ggggccagtg aaggcgcaga ttgacaagtt ccagcacctc tcgacgctgt atccaaatga
     6781 agcgattgtg aatctggcgg aaaaagtctc gcagattacg cccggcgcat tgaaatccag
     6841 tttttttacc agctcaggca cagaagccaa tgaagccgcg attctgctgg cgcgcatggc
     6901 gacaggttcg ttcgatatcg ttgcgctgcg ccacgcttac gcgggcgggt cgtcgctgac
     6961 gaagagcgtg acggcgcaag caccctggag aaaatcggga atcatcagcc tcggcatttc
     7021 ccatgcaatc aatccttatt gctatcgctg cccgctcgga ctgaaatatc cggactgcga
     7081 agtggcatgc gcaaaggacg tggagaattt aatccagacg ggtacgagcg ggcagatcgc
     7141 tgcgttcatg gccgaaccga ttcaaggagt gggcggcttc ataaccccgc cgaaggaata
     7201 ttttaaaatt gttttcaacg ccgtgaaaaa atacggcggc ttatttattt ccgacgaagt
     7261 ccaaaccggc tttgggcgca ccggaaaaaa atggtttggc atcgagcact gggaagtcac
     7321 gcccgacatc ctgacgtgcg ccaagggcat ggccaacggc gcgcccatcg gcgctacgat
     7381 cacgacgaaa gagttggcgg cgtcttatca gggactgaca atttcaacgt ttggcggaaa
     7441 tccggtcacg tccgttgcgg caaaagcgac aattgaattt attgaagagg aaaagctgct
     7501 cgagaattct gaaacggtcg gcaattattt ccgcggcaaa ctggatgagc tacaacagaa
     7561 gcacgcatgg atcggcgacg tgcgcggcaa aggtctaatg ctgggcatgg aactggtgaa
     7621 agaccgcacg accaaagaac ctgctccgga agcaaccgcg caactgctgg aaagaactcg
     7681 cgaacacggg ctgctcatcg ggaagggcgg actgtacggc aatgtgattc gcctttcgcc
     7741 catgctgaac atcggcaagg gcgacgtgga tgaggctgtg cgaatcctgg atatatcctt
     7801 cacggacctc ccgcactagc taaagtgccg tttttgctaa attcgctaat tcttgcgtac
     7861 agagcgataa aaacgcgtgt agcatcttgc aaccaatcgc tatctaaata agccaaaagt
     7921 gaaataaggc tattaaccac agcatgttac aagcaagttc cgttctagaa catccccgca
     7981 actgacatat ttcgtgccga cgctcgcttt gttaacttaa ttacagtttt caaatgaaag
     8041 gatttggtat cgactaaacg cggcagcggg ccgctggccc agttggtcca accattgtac
     8101 ccgatgaaaa agaagaagct cggagaactg ttacgtgacc gcgggatgat ctcctccccg
     8161 gaccttgtaa gtgccattgc ggcgcagcaa gggaaagcta tacatcttgg cgaaatcatg
     8221 ctcgaccggg gctttgtttc caaagaggac ctcggcgcgg cattggaaga agtttggcag
     8281 atcccgtatc tcgattgcgc caccatcact cccgaaaaag atgctctgaa tttggtcacg
     8341 caggccattg cggagcggct gtgcgtcctg ccggtgcgaa ccgagcagag gcggctggtg
     8401 gttgccatgg cagcgccgca gaatcttgca atgctggacg agctacgatt taagactggc
     8461 atggaaattt caccgcgact ttcgtttcgg aaagagctgg agttagcgat tcgcgcccat
     8521 tatggagcga gaacagagcg tgtttcgtcg acggagcaca gcgactctca aaaaatgaaa
     8581 tttgaggaag tcgaattttt ctccacaagt tcgcggcagg ctaatcagga agccatccaa
     8641 gaagtgcaag ctgacctcag acagaaaaaa actcctgcgg ttcgcctggt atccgaagcc
     8701 attcaaatcg cgatggcgaa gcgggccagc gacatccaca ttgagccgcg aaccgcagac
     8761 accgtcgtgc gtatgcgagt cgatggcgta cttcatgatt atcagttcat cccgcggacg
     8821 ctgcaaaact cgttgatatc gagaatcaag atcctgtcgg acatggatat ttcggagcgt
     8881 cgcaatcccc aggacggccg cttcatggtt tccgtcgggg gcaggcaggt ggatttgcgt
     8941 gtatcaacgc tgcccacgca atatggcgaa aaagttgtga tccgcttgct ggatacgagc
     9001 gcgccactca cgtcgtacgc agacctgggc atgccaggcg atgtggctga gggtctcaac
     9061 gagctgctcg cgggccctcg aggcatgatc ctcgtcacgg ggccaaccgg ttctggcaag
     9121 agcacgacgc tgtactcttc tctgaacaaa ctgcgcaagg attcggtaaa tatcgtcacg
     9181 gtcgaagacc ccgtcgaata tgtgcttccg ggtatcaatc aggtgcacgt gaatacgaag
     9241 gctggattga cgtttgtcag ttgcctgcgg tcgatcctac gtcaggatcc aaatataata
     9301 atggtgggcg aaattcgtga caaagaaacc gcggaaattg cgatgaaagc ttcgcagaca
     9361 ggccatgtag tgctgtccac gctgcacacc aacgatagcg tttctgccgt gaatcgcctg
     9421 cttgacttgg ggattcccgg ctacctcatc agcgcttctg tcagtgggat actcgcgcaa
     9481 cgtcttgtcc gcaagctttg cccatgccat tcgcatcaac ccgccacgcc tgcattcaag
     9541 gcgcggatgg tgcagctcgg ctcctccgag gcgcctgcga atatggcggt agcaaacggc
     9601 tgcggaaaat gcaatcacac gggttacgcg ggccgcctgg gaatatacga attgctgcgc
     9661 attgacgatt ccattcgccc gattatccgc gccaatggaa gcgtcgagca gattcgcgcc
     9721 gtcgcgagaa tgaatggaat gcgcctgatg caagaggacg cgttggagaa agtacgaacc
     9781 ggagtaacaa cgctcgaaga ggtcctccgt gttgtgccca tggaaaacgc tactcactcg
     9841 gaatgtcttc agtgcaaaag tcgcattctt ccaacgttta attactgtcc ggcttgcgga
     9901 gcaaaatgca tggcggcacc tgccgctccc aaccggtcaa gcaatcgaga actggttcca
     9961 gaaggaattc tgtagctacg gaaaaacaat aacagtattc aagtacgttc gtccggcgcg
    10021 atgcgcgcca tacacagttt agcgaacgct ctatggcctc cgaaggcgtg tccgaagaag
    10081 tggttttgct tcctcccgat ctcacctctc gcggcatgtt catgcgcgcc atcaaaaatc
    10141 aattcggaat ggtggcggca gacgagtact t
//
LOCUS       DASZMB010000202         6957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1017083, whole genome shotgun sequence.
ACCESSION   DASZMB010000202 DASZMB010000000
VERSION     DASZMB010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6957
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017083"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="VG272_09225"
     CDS             <1..679
                     /locus_tag="VG272_09225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWF13901.1"
                     /translation="GYYDLLLSGSLGKLTEKQRDILQESKESCERLVRLVSMFLNYSA
                     LESGKLVLHLRENDLRDCLSELATRWHEAFVRKHVRLETVLNADIPIFKFDYQKVQQT
                     VANLLDNSLKHTPAGGNVIFRAELYFWERRTAEEIQSLDRRRERVQYPNCIKVSVSDS
                     GPGIASEHHQEIFEDFVRVDRTSSGMGLGLAIAKRLVQAHRGKIWVESEPRRGCTFTF
                     LLPIDNT"
     gene            710..2203
                     /locus_tag="VG272_09230"
     CDS             710..2203
                     /locus_tag="VG272_09230"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWF13902.1"
                     /translation="MADKERILVVDDEPSIRKYLGTLLEVDGYDVETVNSGVEAIQKI
                     EAGDRPDFIILDVLMPELNGIETLTQLMQIDRSLNVVMLSCSNEVGTVVDAIRLGAQD
                     YLTKPFEKAELDVAMLKCRQKQQLRQENKALREYCDTITEDLCFLAASPQMVRIRQQI
                     MQIAPVDVPVFIYGESGVGKEVVARLIHMRSNRRQQPFIKVNCAALPGELLESELFGF
                     EQGAFTGAVRSKPGKFELATKGTIFLDEIAEMSPHLQAKLLHVLQDHQFSRLGGRQLI
                     DVDVRVLAATNVEIQEAMKSGRLREDLYYRLNVLSINVPPLRERASEIPLLFRHFLQK
                     YSEKFNKEPMVPSQHLMEAAVRYPWPGNLRELENFVKRYVILEDDEGSFRELLEMSAT
                     RQRISPREEAAPVREQGLKALVRSLKDEAEMEAIGDALEKTHWCRKDAAKMLGISYKA
                     LLYKIRQFGLDNGRPPRASNKKAPPATNGTPDSGNSSASLAPAGRHG"
     gene            2601..2939
                     /locus_tag="VG272_09235"
     CDS             2601..2939
                     /locus_tag="VG272_09235"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWF13903.1"
                     /translation="MTERRTARRYDLSLPVIIRVPIEKEISSRSGKTRDISTRGVYFT
                     IDQDLGAGAELDITLTLPSEVTRGSEVFIRAMGKVVRIDKRPDSDNSRVGVAAVIERY
                     EIIRTEPSTH"
     gene            3194..3946
                     /locus_tag="VG272_09240"
     CDS             3194..3946
                     /locus_tag="VG272_09240"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HWF13904.1"
                     /translation="MKSPKLDLSIIIPAFNEEGRLPKTLDAVIAYAQSRPYQTEIMVV
                     NDGSSDRTAEIVSAYCQTYPALRLVSNGGNRGKGYSVRHGMLEAAGEIALFSDADLST
                     PVEEADKLLAAIRDQNFDAAIGSRAIDRNMIQVHQSAVREMAGIFFNRMVQWILDIQF
                     SDTQCGFKAFRRERALIVFEQQRIERFGFDPEILYLAKRHGLRVAEIPVRWSHDAATK
                     VNVAADGLLMFLELILIRWNAIRGFYPASPKS"
     gene            4121..4855
                     /locus_tag="VG272_09245"
     CDS             4121..4855
                     /locus_tag="VG272_09245"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWF13905.1"
                     /translation="MSVIIPVYNERAFIEEVLLRVQASPIEKEIILIDDKSSDGTRGL
                     LEDLDKAQSEGKREVYVQNGKARLSLSNIRFLFQQENGGKGAALRRGFEAATGEIILV
                     QDADLEYDPRDYPKLLEPIFEEKADVVYGSRFLGGPQRVHYFWHYAGNKFLTLLSDIF
                     TNLKLTDMETCYKVFRREVLQGIKLKSNRFGFEPEITAKISKGNWRIYEVSISYAGRT
                     YAEGKKITWKDGFSTLWCIIRFNLFD"
     gene            complement(5156..6154)
                     /locus_tag="VG272_09250"
     CDS             complement(5156..6154)
                     /locus_tag="VG272_09250"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWF13906.1"
                     /translation="MYKKFFGLKESPFNVNPDPRYLFLTPYTQEALACLTYGIETRKG
                     FILLTGEVGTGKTTLINKLLEWLHKERVFTAFVFNPRLSVSQFFDFMMADFGIPCESR
                     QKGQMLVKLNQWLLDRYQAGERAVLVVDEAQNLSPQMLEEIRLLTNLETSTEKLLQIV
                     LAGQPELVQKLNQPDLRQLRQRITLRARTRPLTLEETQGYIQERMRIAGAENPDIFSP
                     EAVATVHRYARGIPRVTNLLCEHALVSAFVDQKNPVPAEIVEEVARDFELHIVDPLAQ
                     APAPQPLTQVGLAHANGEGNGDQPPLIESLLQALNTLVDRLNQAEANTESNTEKKT"
     gene            complement(6214..>6957)
                     /locus_tag="VG272_09255"
     CDS             complement(6214..>6957)
                     /locus_tag="VG272_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13907.1"
                     /translation="YALSRRSSITVAASYGFLHFDIPTLQNSEMINVQGGYDYQLDPS
                     DSLGILASYGKIDYTGTGRSTSEYGGALAYGRRITGRLAFQIAAGPQDIRIIVPGGAG
                     NFNLLFVSVNSALTYQRRRGGVSLNFVRGLNGGSGVFLGATSDTASGAAHYQFSRNWT
                     GSINGGTSLNYNLAPAGVARTKFYNWFAGANVGRQLGQHASFNLNYGAMEQNNPIGCT
                     VAVCGGTGVEQTVGVSLNWHLLPVRKEGR"
BASE COUNT         1614 a         1841 c         1895 g         1607 t
ORIGIN      
        1 gggatattac gacctgttgc tttccggttc gctgggcaag ctgacggaaa aacagcgcga
       61 catcctgcag gaatccaagg aaagctgcga acgcctggtt cgcctcgttt ccatgttcct
      121 gaattattcg gcgctcgaga gcggcaagct cgttctgcac ttgcgcgaaa atgatctgcg
      181 cgattgcctg agcgaactcg ccactcgctg gcacgaagcg tttgtccgca agcatgtgcg
      241 cctggaaacg gtactgaacg ccgacatccc aattttcaag ttcgattacc aaaaagttca
      301 gcagacggtc gccaatttac tggacaactc tctgaaacat acgccggccg gaggcaatgt
      361 aattttccgc gcggaacttt atttttggga acgccgcacg gccgaagaaa ttcaatccct
      421 cgatcggcgc cgcgagcgcg tgcaatatcc caactgcatc aaggtttccg tttcggattc
      481 cggcccggga atcgcttccg aacatcacca ggaaattttt gaggatttcg tccgtgtgga
      541 ccgcacgtcg tcgggcatgg gcctcgggct ggcaatcgcc aagcggctgg tgcaggcgca
      601 tcgggggaaa atttgggtgg aaagcgaacc acgcaggggc tgcacgttta cgttcctgct
      661 gccgatagac aatacgtaac gtaattcggg gtacatgaag gtggggcaaa tggctgataa
      721 ggaaagaatc ctggtggtgg acgacgagcc cagcatacga aagtatctgg gcacgttgct
      781 tgaggtggat gggtacgatg ttgagacggt gaactccggc gtggaggcca ttcagaagat
      841 tgaagccggc gaccgcccgg atttcatcat cctggatgtg ttgatgccgg agttgaacgg
      901 catcgaaacg ctcacccaac tcatgcaaat agatcgctcg ctgaacgtgg tcatgctctc
      961 ctgctccaac gaagtcggca ccgtagtgga cgccattcgc ttgggcgcgc aggactacct
     1021 gaccaagccc tttgagaaag ctgaacttga cgtggccatg ctgaagtgca ggcagaagca
     1081 gcagctgcgc caggaaaata aggcgttgcg cgaatactgc gacacaatta cggaagacct
     1141 gtgcttcctc gctgccagcc cgcagatggt gcgaattcgc cagcagatta tgcagattgc
     1201 gccggtggat gtgccggttt ttatttatgg agaaagcggc gttggcaaag aagtggttgc
     1261 gcgcctcatc catatgcgct cgaatcgccg ccagcaaccc ttcatcaaag tgaattgcgc
     1321 cgcccttccc ggcgaactcc ttgaatccga actgttcggt tttgagcagg gcgccttcac
     1381 cggtgcggtg cgatcgaagc cgggaaaatt tgagctggcc accaagggaa ccattttcct
     1441 cgacgaaatt gcggaaatga gcccgcacct tcaggcgaaa ttgcttcacg tcctgcaaga
     1501 tcaccagttt tcacggctcg gcggccgcca gttaatcgac gtggacgtac gagtcctcgc
     1561 cgccaccaac gtggaaattc aagaggcaat gaagtccgga cgcctgcgcg aagatttgta
     1621 ttaccggttg aacgtgctgt cgattaacgt gccaccgcta cgcgagcgtg cttccgaaat
     1681 tccactgctc ttccgccatt tccttcagaa gtacagcgag aaattcaaca aggaacccat
     1741 ggttccctcg cagcacttga tggaagccgc ggtgcgctat ccgtggccgg ggaaccttcg
     1801 cgaactcgaa aattttgtga aacgatacgt cattttggaa gacgacgagg gcagcttccg
     1861 cgaacttttg gaaatgtccg ccacgcgcca gcgaatttct ccacgcgagg aagccgctcc
     1921 ggtgcgtgag cagggattga aggcgctcgt gcgtagcctg aaggacgaag ccgaaatgga
     1981 agccattggc gacgctttgg agaaaacaca ctggtgccgc aaggatgccg ccaagatgtt
     2041 gggcatcagc tacaaggcgt tgctctataa aattcgccag tttggactgg acaacggtcg
     2101 cccgcctcgc gcgagcaata agaaggctcc gccagcgacc aacgggacgc cggactcggg
     2161 gaattcgtct gcgtctttgg cccctgctgg ccggcacggc tagtgcggct agtacatgca
     2221 aagttttggt catagaagag tgagcggttt ccggaatttg ccttggtggg atttttcatg
     2281 ggcacgcatg gagacagaaa gacgaagttt taaattaaat tttgttcatt ttcgttccgc
     2341 attgaccacc gcaggaggaa tgaaaattga gcaccattcg gtagtgaaca gtttacgaaa
     2401 acggctcagc gtaaggcttc agttctatag tgcttacagg ggcggcgtat ccaagttgaa
     2461 acgtcgggac gtattgctat ttaatcaaag attgaattca gtcataatgt aaattgaact
     2521 accgctttgg aacgccgatt gcttcttatt gatagtcaag ttgggactgc caggtagatc
     2581 aggttcgggg gaacaagaag atgacggagc ggagaacagc acgccggtac gacctttctt
     2641 taccggtcat tattcgcgtg ccgattgaaa aggaaatttc ctcgcggagc gggaaaaccc
     2701 gcgatatttc aacgcgtggt gtttatttta caattgatca ggacttggga gccggcgcag
     2761 agttggatat cactctgaca ctgccttccg aagtcactcg cggttccgag gttttcattc
     2821 gcgcgatggg caaggtcgta cgcatcgata aaagaccgga cagtgataat tcccgcgtcg
     2881 gcgttgccgc ggtcatcgag cgatacgaaa tcattcgcac agaaccttcc acgcactaat
     2941 cctactttca tttaatatcg cctccatcca ttgcgccctg gggtaattct cccccaggga
     3001 tttttgggtc tggacgaatt tttgcttttg cctcatctga atgtctgccg gtttattcct
     3061 gccagtgcgc ctttctcact ggaacgtata ttctctggga tttcgattcg tggacgaatg
     3121 ccatagcaga aaatataatc tcgccgttgg gagacgttgt gcctgtcgcg aagttcccgc
     3181 ggcgccacct ctaatgaaat cgccaaagct ggacctttcc attattattc cggcgttcaa
     3241 tgaggaaggt cgcctgccga aaacactcga tgcggtgatc gcctacgcgc aatcgcgccc
     3301 gtaccagact gaaatcatgg tcgtgaatga cggatcttct gaccgcaccg ctgagattgt
     3361 aagcgcctac tgccaaacct acccggctct gcgactcgtc tccaacgggg gaaatcgtgg
     3421 aaagggatac agcgtccgcc acggcatgct tgaggcggcg ggagaaatcg cgctgttcag
     3481 cgatgccgat ctctccacgc cggtcgagga agcggataaa ttgctcgccg cgatccgcga
     3541 ccagaatttc gacgccgcaa tcggatcgcg cgccattgat cgcaatatga ttcaggtgca
     3601 ccaatccgcc gtccgtgaaa tggccgggat tttctttaat cgcatggtgc aatggattct
     3661 agacattcaa ttttccgaca cgcaatgcgg attcaaggct tttcgacggg aacgcgctct
     3721 catcgttttc gagcagcagc gcatcgaacg ctttggtttc gatccggaga ttctttatct
     3781 cgccaagcgc cacgggcttc gtgttgcgga gattccggtg cgctggtcgc atgatgccgc
     3841 gaccaaagta aacgtggcag ccgacggact cctcatgttt ctggaattaa tcctgatccg
     3901 ctggaacgcg attcgtgggt tctaccctgc ctcgcccaag tcttaaagaa tcacagagac
     3961 agtgtcagct gagcaaaaca ctactccaaa tcatttgcgt tccgtgcatg catattcaat
     4021 catgtggtcc gaaagccttt gtgggcttgg cattcgcctg taaatggttt tacaaactga
     4081 aggggagatg cgtaaagggg atgcgtgaat cggtatgaaa gtgtctgtca taatcccggt
     4141 ctataacgag cgagctttta tcgaagaggt cctgctgcgc gtgcaggcat cgccgatcga
     4201 aaaggaaatt atcctgatcg acgacaaatc cagcgacgga acgcgaggac tgcttgagga
     4261 tctcgacaag gcgcaatcgg aaggcaagcg cgaagtatac gtgcaaaacg gaaaagcccg
     4321 tctatcgctc tcgaatattc gtttcctgtt tcagcaggaa aacggcggaa agggcgcagc
     4381 gctgcgccgc gggtttgaag ctgccacagg agaaatcatt ctggtgcagg atgcggatct
     4441 tgaatatgac ccgcgcgatt acccaaaact tctggaacca atttttgaag aaaaagcgga
     4501 cgtcgtctac ggatcgaggt ttctgggcgg accgcagcgc gttcattatt tctggcacta
     4561 tgccggaaat aaatttctaa ccctgctctc ggatattttt acaaatctga agcttacgga
     4621 catggagacc tgctacaagg tcttccgccg cgaagtcctg cagggcataa aattaaaatc
     4681 caaccgcttc ggattcgagc cggaaattac cgcgaaaatc tccaaaggca actggcgcat
     4741 ttacgaagta tcgatttcct acgccggccg cacctacgcc gaaggcaaga agatcacgtg
     4801 gaaagatgga ttttccacgc tttggtgcat cattcgcttt aatctgttcg attgaaggtg
     4861 ggacaggctt cagcctgtct gggttacgtc taattcatgc aaatctaaac aaatgggctg
     4921 aagcgtctcc tactgaggtt acttcgcgaa atacgctgcg tggtttgaag atttccttgc
     4981 catttccgat cggcgaacgg gtcaaattta cagaaccgcg gcatccattg gcgcaggacg
     5041 tcttgtacgc gcgatgcttt ctcatcttca ggacaacagg gtgctcgtac tcagaaaacc
     5101 aatttcatcc gcattgacca atttggccat ggcggaactc aactgaacgt gcgggttagg
     5161 ttttcttttc ggtgttggat tccgtgttgg cttcggcctg attgagacgg tctacaagcg
     5221 tatttagcgc ctgcaggaga gattcgatca gaggcggttg atctccattg ccttcgccgt
     5281 tcgcatgggc cagtcccact tgagtaaggg gttgcggagc gggagcctgc gccagcggat
     5341 cgacgatgtg caactcgaaa tcgcgcgcca cttcctccac aatttcggcc ggcaccggat
     5401 ttttctgatc gacgaatgca ctcacgaggg cgtgctcgca tagcagattc gttactctgg
     5461 gaattccgcg agcataccga tgcacggtcg ccacagcctc cggactgaaa atatccggat
     5521 tctcggcgcc cgcgatgcgc atgcgctctt gaatgtatcc ctgcgtttct tcgagcgtca
     5581 gggggcgggt gcgcgcgcga agcgttatgc gctgccgcag ctgtcgcagg tccggctggt
     5641 ttagtttttg cacgagttca ggttgccccg cgagcacgat ctggagcaat ttttcggtcg
     5701 acgtctccag attcgtcagc aggcgaattt cttccagcat ttgcggtgaa agattctgcg
     5761 cttcgtctac gacgagcacc gcgcgctctc cggcctggta gcgatccagc agccattggt
     5821 tcagcttcac cagcatttgt cccttttgcc gggactcgca gggaatgccg aagtccgcca
     5881 tcataaaatc gaaaaattgc gaaacgctca agcgcgggtt aaacacaaac gcggtgaata
     5941 cacgctcttt gtgcagccat tctagcagct tgttgattaa tgtcgttttg cccgtgccta
     6001 cttcaccggt gaggagaata aatcctttgc gagtctcaat cccatacgtc aaacaggcca
     6061 acgcttcctg cgtgtacggc gtcaggaaga gataacgcgg gtcgggatta acattgaacg
     6121 gactttcttt gagtccgaaa aacttcttat acatgccgta acctcaaaat ctccattggt
     6181 cgtccattac ccgctgtact tgagcgcatg gtattaacgg ccttctttgc gaactggcag
     6241 caggtgccag ttgagcgaca ctcccacagt ctgttccacg cctgtgccgc cgcatactgc
     6301 caccgtgcat ccgatcggat tgttctgctc catcgctcca taattcagat tgaatgacgc
     6361 gtgttgaccc aattgacgcc cgacattagc gcccgcgaac caattataga atttcgtgcg
     6421 ggccacgcca gcaggggcca gattgtagtt taaagaagtt ccgccattga tcgagcctgt
     6481 ccaattacgc gaaaactgat aatgcgccgc gcctgaagcg gtatcgctcg tcgctccgag
     6541 gaaaacgcct gaaccgccgt tcagcccgcg cacaaaatta agcgaaactc cgcctcgacg
     6601 cctctggtac gtcagtgcgc tgttcaccga aacgaacagc aagttgaagt ttcccgcacc
     6661 gccgggcacg atgatgcgaa tgtcctgggg tcctgcggca atctggaatg ccagccggcc
     6721 ggtgatcctt cgaccgtaag ccagcgcgcc gccgtactcc gatgtggatc tgccggttcc
     6781 ggtgtaatca atcttgccgt agctggcgag gatgcccaaa gaatccgacg ggtcgagctg
     6841 ataatcataa ccgccctgca cgttgatcat ttcgctgttc tgcaaagtag gaatatcaaa
     6901 gtgcagaaat ccgtaagacg ctgccacggt gatggacgat cggcggctca acgcata
//
LOCUS       DASZMB010000203         4693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_504967, whole genome shotgun sequence.
ACCESSION   DASZMB010000203 DASZMB010000000
VERSION     DASZMB010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4693
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504967"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..3334
                     /locus_tag="VG272_09260"
     CDS             170..3334
                     /locus_tag="VG272_09260"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HWF13908.1"
                     /translation="MPERSLRQNLFSITLVCFFLSGAAGLIDQVVWSKALGLIFGHTA
                     YAVATVLAVFMAGLASGSAWIGRQGDRWNRPIVLYAWLEFGVAATAAISLAGLAGVRA
                     VYVAAYPYAAGHASTLLALRFAGSALVLFLPTFLMGGTLPVLVRGVTQTSAQLGTHLS
                     RLYWINTAGAVAGTFAAGFLFLPSLGLRRTLAIAVTLNLIAGVLAFRLSREEGNLSPA
                     AQPLISEKDDLPTSFAPSKFLLVSFAVVGATAMSYEIGWTRLLSTQLGSSTYAFTLML
                     GTFLTGIVLGSALFERWNRRHEPSAMTFAFTQTLTAFAALLFLIFFTHLIEVLPPILR
                     ATHESFRGLVLAQLVTSALAMLPTAVVFGFNFPAVVVLIADPPSRSEHSAGAAVGRAY
                     AWNTLGAIVGALAAGFWLMPRLGSFHLLAVTAGLNLILAAAISWKSTLRSNWKIPAFA
                     ANLILVAFAIVIGFSNYFYDPAVASFNTLMYWNLYDRPLTLRENAHALDIVYFRDGLN
                     ANISVARTGDYVALRTNGKVDASNHDAATQLLIGHLGALAHAPRRVLIVGFGSGMTAS
                     ALVGYPELERLDIVEIEPAVVGAAPLLAQLNRNVLLDPRVHVTFDDARNFLFTTRDKY
                     DLIISEPSNPWIAGVATLFTREFYAAVHSRLTSDGVFVQWMQAYSLFPEDLRMLFATF
                     LSEFHGATLWHGDAPDLILMAPSPASAEIMRRARSLYESPHLHEDFVQLGMQEPAGLW
                     GFYLLDDPGLRKFSDGAQIDTDDQTLLEYHAPRSLLVHGLEDKNRDAILLQQTNPLPA
                     DLPRDARDASLAASAETAVNQEDGNGAERFLRALDNRPATAAIQIIRGRAALADANFQ
                     TSLHAFDAALAIDPSSVEAAWGVAESNRRFGNNQKARQMFEEILTRDPRNARAMASLV
                     KLEIDFSRWPEAESLQRRIMTAAAHPDTAANAQLAEILLHEGKLDEAYRAIMDCLAED
                     RYSYQAHLNLGKLLAQQNKWAEARKHLEFVMRFYPDENSEVYPLLFTADKTLGDPLAA
                     ARAVKFGLRVFPDNSELQRLKPLL"
     gene            complement(3637..4455)
                     /locus_tag="VG272_09265"
     CDS             complement(3637..4455)
                     /locus_tag="VG272_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13909.1"
                     /translation="MLEQENGQMTEGSPRWMGIAVVGLAILSAVGVGMAWNAGGHAHE
                     AEQAIAAQNKTFQAAEDGFSQRMAQAEHTNAELQSEVNLVGDKLKLTEEQLSSARSQV
                     KQSRADYTKKLNDVQTTLATKASTDDVKALGTDVNGVKTDLDATKGNIQQLRGEHGEL
                     IARNHEELEQLKRMGERDYFEFTLSAKGQKEHVGQTQIEFRGASAKKHQYTIAMYVDD
                     MRLEKKNKAVNEPIYFYAGGSRQPMELVVNQVSDKKISGYLSAPKAIAASAKVN"
BASE COUNT          967 a         1313 c         1218 g         1195 t
ORIGIN      
        1 ccattcaacg tgtagaagga gcttaaattt acagtagtga aataatactg gggttcagag
       61 cgggaaatac cgtaggttca ggcaaggcta tccagacatt agacgcacca caagtatata
      121 tggcgcggaa ttgacgccgc gaaacgccca gactaccatg cggcccgtca tgccagaacg
      181 atccctgcgc caaaatttat tttccatcac gcttgtttgc ttttttcttt ccggcgccgc
      241 cggattaatc gaccaggtgg tttggagcaa agcgctcggc ctgattttcg gccacaccgc
      301 atacgctgtc gccacggttc ttgccgtgtt catggccggc ctggcttcgg gcagtgcctg
      361 gatcggacgg caaggcgacc gttggaacag gcccattgtt ttatatgcgt ggctggagtt
      421 tggagttgcc gcaaccgccg caatttcttt agcagggctc gccggggttc gcgcggtgta
      481 tgtggcggcg tatccctacg cggcgggtca tgcgagcacc ttgctggcgc tgcgtttcgc
      541 agggtctgca ctggttttat ttctgccgac ctttctgatg ggcggcacgc tgccagttct
      601 ggtgcgcgga gtcacgcaaa cttccgcgca gcttggaact catttgtcgc gcctgtattg
      661 gatcaacacc gccggtgcgg ttgccggaac atttgccgcg gggttcctgt ttttgccttc
      721 gttgggcctg cgacgaacgc tcgcaatcgc cgtcacatta aatctcatcg cgggagtatt
      781 ggctttccga ctctcccgcg aggaaggcaa tctatcgcct gctgctcagc ctctcatttc
      841 tgaaaaagac gacttgccaa cttccttcgc accatcgaaa tttctgctcg ttagttttgc
      901 agttgttggc gcaacggcaa tgtcgtacga aatcggctgg acacgcctgc tttccacgca
      961 acttggcagc tccacctacg cgttcacgct aatgctggga acatttctta cgggaattgt
     1021 tctgggaagc gcgctatttg aaagatggaa tcgccgacat gagcccagcg caatgacttt
     1081 tgcgttcacc cagacgctta cggcttttgc ggctctcctg tttctcattt tcttcacgca
     1141 cctcatcgaa gtgcttcccc cgattttgcg cgccacgcat gaatcgtttc gcggtctcgt
     1201 gctcgcgcaa ttggtgacca gcgcgctggc catgctgccc acagctgtgg tcttcggctt
     1261 caattttccc gccgtcgttg tcctgattgc cgacccacca tcccgaagcg aacacagcgc
     1321 cggcgccgca gtaggccgcg cctacgcgtg gaatacgcta ggtgcgatcg tgggcgcatt
     1381 ggccgcgggc ttctggctga tgccccgcct cggaagtttt catttgcttg cggtgacggc
     1441 aggtttgaac ctcatccttg ccgcggcaat ttcatggaaa tccacgctgc gatcgaattg
     1501 gaaaatcccg gcgttcgcag cgaatcttat tttagtcgcc ttcgcaattg ttattggctt
     1561 ctcgaattat ttttacgatc cagcagtcgc ttctttcaac acgctgatgt attggaatct
     1621 ttacgatcgc ccgctcacgc tgcgggaaaa tgcgcacgca ttagacattg tttattttcg
     1681 agacggcttg aacgccaaca tttccgtcgc aagaacggga gattacgttg ccctccgcac
     1741 gaatggaaaa gtggatgcct cgaaccacga tgcggccacg caactcttga tcgggcatct
     1801 cggcgccctc gcgcatgcgc cgcgccgtgt cctgatcgtc ggtttcggca gcgggatgac
     1861 ggcgtccgcg ctggtcggct atcccgaact cgagcgcctc gacatcgtag aaattgagcc
     1921 ggccgttgtc ggcgcggcgc ccttgctcgc acaattaaat cgcaatgtgc tgctcgatcc
     1981 acgcgtgcat gtcacttttg acgacgcgcg caattttctt ttcaccacgc gcgacaaata
     2041 cgatctgatt atttccgagc cttccaatcc atggattgca ggcgtcgcca cgctttttac
     2101 gcgcgaattt tacgcagctg tgcacagcag gctcacttca gacggcgtct tcgtgcaatg
     2161 gatgcaagcc tattcgctgt ttccggaaga tttacgcatg ctgtttgcaa ccttcctttc
     2221 cgaatttcac ggagccacac tttggcacgg tgacgccccg gacctgattc tcatggcgcc
     2281 cagccctgca tccgccgaaa tcatgcgccg tgcgcggtct ttatacgagt cgccgcacct
     2341 tcacgaagat ttcgtgcaac ttggcatgca agagcccgcg ggactatggg gcttttactt
     2401 gctggacgat ccgggtctgc gtaagttcag cgacggcgca caaatcgata cggacgacca
     2461 gacgctactg gaataccatg cgccgcgatc tctactcgtt catgggctgg aggacaagaa
     2521 ccgcgacgca attttgctgc aacaaacaaa tccgcttcct gcagatttgc cgcgggacgc
     2581 acgcgatgcg tcgctcgccg cctcggccga aactgcggtc aatcaggagg atggcaatgg
     2641 agccgagcgg tttttgcgtg cgctcgacaa tcgtccggcc accgccgcca ttcaaattat
     2701 tcgtggccga gccgcgctag ccgacgccaa ttttcaaaca tcgcttcatg ctttcgacgc
     2761 agcattggcc attgatccca gttctgtgga agccgcatgg ggagtagccg aatcgaatcg
     2821 ccgcttcggc aacaatcaaa aagcgcggca aatgtttgaa gaaattctaa cgcgagatcc
     2881 ccgaaatgcg cgcgccatgg cttcacttgt aaaactggaa atcgattttt cgcgctggcc
     2941 cgaggctgag tcattgcagc gccgcatcat gactgcagcc gcgcatcctg ataccgcggc
     3001 gaacgcacag cttgccgaaa tcttgctgca tgaaggcaaa ctcgatgaag cctatcgcgc
     3061 catcatggat tgcctggcag aagatcggta cagctatcaa gcgcacttga atctcggcaa
     3121 gttgttggcg caacaaaata aatgggcgga agcaagaaaa cacctggaat tcgtaatgcg
     3181 attttacccg gatgaaaatt cggaagtgta tccactgcta tttacagcgg ataaaactct
     3241 aggcgatcct ttagccgcag ccagagcagt gaagtttggc ctgcgagtat tcccggataa
     3301 ttccgaattg cagcgcttga aacctctatt gtgaatagct tttagtagcg cgggcttcag
     3361 cctgcggggg ttagtccctg ctaggaccaa attccgaagg ctgaacagtt tgcggaaaaa
     3421 gtagtttatt ctgtcattcc gagcgaagcg aggaatcttt cttcgattta agtataagaa
     3481 aataaagaag gagagattcc tcgcttcgct cggaatgaca aaatgttgag tttttccgta
     3541 acttgtgaag cccgcgctac taaaaccaaa tagaaagggc cgccggagcg gcccttctta
     3601 ttcacaacct gcattagtta tgaaatgggt tagttttcag ttcacttttg cggaggctgc
     3661 gattgctttc ggtgcactca ggtagccgga aatcttcttg tcgctgacct ggttgaccac
     3721 caattccatc ggctggcgcg atccgccggc gtagaagtaa atgggttcgt tgacggcctt
     3781 gttcttcttt tcaagacgca tgtcgtccac gtacattgcg atcgtgtact ggtgcttctt
     3841 ggcgctggcg ccgcggaatt caatctgcgt ctggccaaca tgttccttct gtcccttggc
     3901 agacagagta aattcgaagt aatcgcgttc gcccatgcgc ttgagctgct cgagttcttc
     3961 gtggttgcgc gcgatcaatt ccccgtgctc gccgcgcaac tgttggatgt ttcccttggt
     4021 tgcgtcgaga tcggttttca cgccgttcac gtcggttccg agagccttga cgtcatctgt
     4081 gctggctttc gttgcaagag tcgtttgcac atcattcagc ttcttcgtgt aatcggcgcg
     4141 gctctgtttc acctggctgc gagccgagga caattgctct tccgttaatt tcaatttgtc
     4201 gcccaccaga tttacttcag actgcaactc tgcattggtg tgttccgctt gcgccatacg
     4261 ctggctgaac ccatcctcgg cagcttgaaa agtcttgttc tgcgctgcaa tcgcctgctc
     4321 tgcttcgtgt gcgtggcctc cggcattcca ggccatgcca acacccactg ccgagagaat
     4381 cgccaggcca accacagcta ttcccatcca gcgcggtgaa ccttccgtca tctgcccatt
     4441 ctcttgttct aacatttgct tgatctcctt tgcgatttcc ctcgcgccta gggaagtgca
     4501 tgtagcgaac caaaggctaa tttcgccagc catagactgg acatcttgct gaaatcaaat
     4561 tagttagagt gcttttcagg agcacaaagt aatgaaatta ttgtggtatt caccgtgtaa
     4621 gttttacttg gggaataggg tatttcactc ataaattcca gattcagctg gatttacagt
     4681 ctcacacaca aac
//
LOCUS       DASZMB010000204        16157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3753674, whole genome shotgun sequence.
ACCESSION   DASZMB010000204 DASZMB010000000
VERSION     DASZMB010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 16157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16157
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3753674"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="VG272_09270"
     CDS             <1..1090
                     /locus_tag="VG272_09270"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF13910.1"
                     /translation="SRVGRRDFVKMAAGGAMAAALPVSAAAQGKEKGVDWWDAHPGKN
                     GPGKPAAIDCHAHWSPDAYNKVLAEFGHPLANNNPLNTDLDARIKWMDEHEVEMHCLT
                     LSGAMPWQWSSAEQGAQLAQVINDVGIEACKKYPSRFVLGAELPIRDPQLTLKELNRV
                     AGKPGVRAVHLPDSVERHDYIVDPNFEPVLARIEELGYPIVFHQMDGEANTYGGPRVM
                     GKINLAAGLDAPIDHTVLATKLMMAGTVDKYPKLEMILPHAGGAFPYLAGRIEHFFYH
                     FNGQQVTLAHPFRDYLRRFYYDYLIYYPEAFQFLVTMVGTDRIVVGTDIFAAQDVQYP
                     AEVLDQFKLPAPERERILKGNAIKLLHL"
     gene            complement(1183..2295)
                     /locus_tag="VG272_09275"
     CDS             complement(1183..2295)
                     /locus_tag="VG272_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13911.1"
                     /translation="MKTDFIRIAAMCAGLTIAGGAVWVGSARAQESAPKFEVDPMWPK
                     PLPNLWVTGGVGGLCIDSHDHVFILNRRDLTDNDLDAGRQAPVVIEFDPAGNVVNSFG
                     DPSTTPDTPHGCTLDKEDHLWITGTGDGIVQEYTHDGSKLLLQIGKMGMFDSSDGTAK
                     GKPLNAGQSSFFKPAGISIDPKDGDVYVADGETVGGNHRIAVFDKDGKFLRQWELNRT
                     ASETGNSFVPILHCVTMDNAGLVYVCDRRGHRVQVFDKTGEFQKNIPVAFTATSSLPP
                     GPGHESGILGSTVSVGFSQDAGQKYMYVVNQDNEKIDILDHATGKLLSSFARVGRQPG
                     QLTYAHFLAVDSKGNIYVGEVGTGKRVQKFNMAKGK"
     gene            complement(2418..3230)
                     /locus_tag="VG272_09280"
     CDS             complement(2418..3230)
                     /locus_tag="VG272_09280"
                     /inference="COORDINATES: protein motif:HMM:NF044772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWF13912.1"
                     /translation="MNRTIAIRSGLFVALAVSALSVLAFIPVSYGAPRTPQATDTSKK
                     WVAPDPATIPAGPLGDSIRLGMNVFNDTPKYAEKYVGNKLSCTHCHVDGGTVSQAMPM
                     VGLPGIFPMYRDREKTVITFEARIEQCFQRSESGHRVPNDGPEMTGLVAYSQWLSRDQ
                     VTGKPFPGRGLVTLPELNGDPVNGGKIFGEQCAVCHGADGAGKLPVAPALWGPNSFSD
                     GAGMNQISKMAAFVQHNMPQNAPDTLTPQEAYDVSTFIHSKPHTPFVMKDHM"
     gene            complement(3279..3692)
                     /locus_tag="VG272_09285"
     CDS             complement(3279..3692)
                     /locus_tag="VG272_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13913.1"
                     /translation="MGKRKWVFISAVGMLAVLVGQGIYADFNAGSDKSASPQSVHQAP
                     PTAAPAQAAYNVGPNPAYSPDLAEGDGKNEVAYNCGLCHALAYITTRPPMSAQAWEHE
                     VNMMRGFYGLSVSDSTAQKMIQYLGTHYTPETMKR"
     gene            3931..4713
                     /locus_tag="VG272_09290"
     CDS             3931..4713
                     /locus_tag="VG272_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13914.1"
                     /translation="MNVRTLSSAATCAILLFSCSLFAQTGAKETRVMIRAVARDAKVI
                     GTHVGGAKITVKDAATGEILAQGVQQGGTGDTNTIMKTPRTRGMSVFSSADASGYLAV
                     LHIDKPTVVEISAEGPLGNSQATQRTSKTMLLVPGEDVLGEGILLEIHGFIVTAVSPL
                     ADAKVKAGAPFEVKATVTMACGCPTEPDGLWDANKIHVVARLLRDGKIQSEIPMTYAG
                     VQNTFHADVPATAAGPMELQVLSMDAANANFGILKEAVTIVP"
     gene            4831..6192
                     /locus_tag="VG272_09295"
     CDS             4831..6192
                     /locus_tag="VG272_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF13915.1"
                     /translation="MERALTVSEMIDQRPLSRYQIWAMALCATVVVLDGFDTQSIGFL
                     APSMAETLHIPIKTFGPVFGAALFGLMISSIVIGPLADRWGRKWPIVISTLIFGAFAM
                     LTPRATSLPELWAVRFVTGLGLGGAITNVLALMSEYSPKRHVAVLVSILFCGMPAGAL
                     LGGLISSFMLPRWGWQSVFYAGGILPFLLALLLIAILPESVRYLEISGADPSRISKIL
                     ARISPDLGTAPFSRSPLQTQHRNAPAVRLFTEGRAVGTLLLWVPYFMNLLILYFVVSW
                     LPALLRQKGSPVSAGITAIILFSVGGIAGSATEGFLINRWSAFTILLSEFLCSAALIG
                     SLAYSTSLPLTMTITLVLGFTVQAAQGGLNVMAATIYPTSIRATGIGWALGIGRVGSI
                     VGPVVVGVMLKLNWGPREIFQAGAIPALCAAAATLLGLWQRRDASTGPHTPEAMKQPS
                     LSH"
     gene            complement(6230..7096)
                     /locus_tag="VG272_09300"
     CDS             complement(6230..7096)
                     /locus_tag="VG272_09300"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HWF13916.1"
                     /translation="MLRGGVSSRAQEFELKSRTVDTPDIRLGTSAFTAAGWEGTFYPA
                     GMKPADFLSYYATKFDTVEVDSTFYRTPAISTVKGWYAKTPPGFLFAAKVPQLVTHEK
                     MLADCDEEFHGFLRAMDNLGEKLGPMLLQFGYFNKQAFSGVQDFLARLVPFLKKIPRE
                     YRFALEIRNKYWLVPAFVDVLREHNVALALIDQSWMPGPKQQFARIDPITSDFAYVRW
                     LGDRKGIELQTKVWDKTILDRTAELGEWVDVLRKVRERRIPIQAYANNHYAGYGPGTI
                     EQFRKLWQSATP"
     gene            complement(7110..9341)
                     /locus_tag="VG272_09305"
     CDS             complement(7110..9341)
                     /locus_tag="VG272_09305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWF13917.1"
                     /translation="MRPKWPVAILSVCFISAGLSAGQSLSQSATPGAPSAQKEAKQSD
                     KGESGYVLKVTTRLVTLDVVATDSHGNAIRDLRPEELQIFEDRKAQQKIEHFEFLETL
                     NRPGTSENSASAARKAAGIISNQLPLDQLRIPPTVLLMDSLNTQTPNQMQGRAKMLQL
                     MRSLPPDTPVAVFLLGSSLRILQGFTSDGRLLRAALDRTLLGNSIQPDPRNNLSDTSN
                     YVQDQTAGMDVNMGVQAQLGEVQNFEKEEYSLSVDLRVKETLGALSQIGQYLSGIPGR
                     KNLIWVSESFPLTIVPDPGTGNNPFGGSREYSEEIKIVGNQLTDAQVAVYPMDVRGLQ
                     AQQSLSASQNINLPETRLTGPAIANRLSVEDTDFQQTLGTMDEIAQDTGGKSCKNSND
                     LAGCLMTALKDSSSYYEMSFYPSKANWDGRFHKIVVKTSRPGVKLVYRRGYYALDAAS
                     LAKRQPPQEHLRQACEDELPSTTIPLTVQPMTAGPNGNAPAGLQYMLLISAAGLSATQ
                     AGDSYNMNLRLADCEFAQKPTVFRFNERELPKTVTSEVFRGWQASGIPDHIALMPSAD
                     TRRIRLVVLDVDSGLTGAIDIPVLPSEIAKASVRVAPPAPVVTTPYVEFPDKAKGPSQ
                     PKIVESLTFRLNASSAGTLNWKGDSLIYQLEGDMPLNKSAKAFSGYAFGGRFHCQDGH
                     FVAVDAADGEPALRFTFHNHSGKVLVVDLKGDQPVYVGDLPVDASAQTFFEQVWKGSR
                     CQE"
     gene            10203..11162
                     /locus_tag="VG272_09310"
     CDS             10203..11162
                     /locus_tag="VG272_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13918.1"
                     /translation="MSAVSIAARAQQDQPNNYPNNGADTREQRNDAPPPDSQMQPPDP
                     NDQQQQNLQQDQVPAPQDQVQSSQPVPPETLTIPAGTLVRVRVDEWISTDRNVTGDSF
                     SANLDQPLVVNGWVVARRGQAETGRVSLVKKGGHGNGSSQLGLDLPDLTLVDGQQLPL
                     QTELFQTSAGSSAGRNAATVGTTTGIGALIGAVAGGGTGAAIGAGIGAGAGIAGVMST
                     PGKPTVVSPETVLSFRLRNPVTISTTNSQFAFQPVSQSDLDSRPRENRPQMRRPVPPP
                     DYGYPYAYGYPGYGYGYPYGWYPAPVIGFGYYGGYGRFRGFRR"
     gene            complement(11232..13307)
                     /locus_tag="VG272_09315"
     CDS             complement(11232..13307)
                     /locus_tag="VG272_09315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HWF13919.1"
                     /translation="MTRNYNPPAPFSPVTVEFDREMSGDLHDSETREWLVTNGIGGYA
                     FGTVAGLQTRCYHGFLMAAVQPPSGRTLLVAKLDETARYAFKNFALSTNRWADGTLAP
                     QGFLNIERFHLEGTTPVWTFALADALIEKRIFMASGENTSYALYRVVRAGSPVEISIK
                     LLADYADEHSVTVGHTHPMSVTNIEHGLKVVAFEGATPFFVVSDTAAASLASNWYRNF
                     DLSAEHERGLPDVTDHFFAGELRATIAPGASLTIVASTEAAASLDGEAAFAKRQKEER
                     ALLAKFSAANVAGTKGNSQSVPPAVSQLVLAADQFIAARPLADAPNAKTILGGYPWFN
                     DWGRDTMVALPGLCLVTGRPWLARNILRTFSHFVSEGMLPNQLPAAGVAPSYNSVDAT
                     LWYFKAVRQYFESTSDIGLLHELYPALEEIIEAHVRGTRFHIHVDPADGLLFAGEPGV
                     QLTWMDAKVKGRVVTPRIGKPVEVNALWLNAAATMARFAKIVGSDAARYETIKNNARA
                     GFAKFWNAEKQYCFDVIDAPGSPDGKDATLRPNQIFAVSLPETALNADQQRAVVEVCA
                     RELLTSFGLRSLGPDEPGYRGHYGGTAEERDDAYHQGTVWGWLLGPFALSHLRVHGDA
                     RQAMSFLEPLLSEIKSGGLGTLSEIFDGDPPHFPRGCVAQAWTVGETLRAWRSIAVAV
                     ERESGAPTK"
     gene            complement(13376..13768)
                     /locus_tag="VG272_09320"
     CDS             complement(13376..13768)
                     /locus_tag="VG272_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWF13920.1"
                     /translation="MKHRILVVDDDAQSRESLRKWLESEGHEAVLAESLEEGFVKIAD
                     PPMPSVVLLDIQLGIHSGLTLVHWVRRQNYLAGLPIAAMASFASFKEFKRVEEAGCDT
                     CFTKPIDLGSLRTYLVGLHIFSDSKIVK"
     gene            complement(13932..14729)
                     /gene="surE"
                     /locus_tag="VG272_09325"
     CDS             complement(13932..14729)
                     /gene="surE"
                     /locus_tag="VG272_09325"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HWF13921.1"
                     /translation="MAHILITNDDGIQAEGLRALVQAVQGLGTISVVAPAHERSAAAQ
                     SLTLRHPIFWDQVAEHEWSVEGTPADCVILALNKLLPQRPDIVISGVNRGGNLGENIF
                     YSGTVGAAMEGAINHIPAIAVSAVHRGRVFRFDQAAKLARDLAQMALVEGMPLGTMLN
                     VNVPQLWNGNVRMTRQSKKVTRNVLQEGTDPRGRNFFWLLEQEHIEGLDPQSDYAAVF
                     DGAASITPLHLDRTHEISLDHLSQWASKLESDAENEEPATVARKSLK"
     gene            complement(14970..15046)
                     /locus_tag="VG272_09330"
     tRNA            complement(14970..15046)
                     /locus_tag="VG272_09330"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15010..15012),aa:Pro,seq:ggg)
     gene            complement(15194..15691)
                     /locus_tag="VG272_09335"
     CDS             complement(15194..15691)
                     /locus_tag="VG272_09335"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWF13922.1"
                     /translation="MEELENNGSGNGHGGALEAAIPGKELFRIGEVSRVTATKPFVLR
                     YWETEFPMLQPVKSPKGHRLYRQEDIDTVFTIKRLLYNEGFTIAGARRHLRDQALGTG
                     AGEGANGGLLANATPAMHANHGEEHAPPQAAPALSPHLAVFNRQTLLDLRDSLRSLLT
                     ALESE"
     gene            complement(15963..>16157)
                     /locus_tag="VG272_09340"
     CDS             complement(15963..>16157)
                     /locus_tag="VG272_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13923.1"
                     /translation="ERFLENLLRKSFDFEGTPIRFLQRMKEKRDWTKSSTRRTPVPKH
                     PGMSHNRVKRTPRKEEPVEE"
BASE COUNT         3551 a         4838 c         4188 g         3580 t
ORIGIN      
        1 ttcgcgcgtc gggcggcgtg attttgtaaa aatggctgcg ggcggagcca tggctgcggc
       61 ccttcccgtg agcgcagccg ctcagggaaa agaaaaagga gtggactggt gggacgcgca
      121 tcccgggaaa aatggaccgg gaaaaccagc ggcgattgat tgtcacgcgc actggtcgcc
      181 ggatgcctac aacaaagtat tagccgagtt tggccatcct ctcgccaata acaatccgct
      241 gaataccgat ctcgacgcac ggataaaatg gatggacgaa cacgaagtgg aaatgcattg
      301 cctcactctt tcgggcgcga tgccctggca gtggagttcc gccgagcaag gcgcgcaact
      361 tgcacaagtc atcaacgacg tgggcattga agcctgcaaa aaatatccct cgcgttttgt
      421 gttgggcgcg gaactcccga ttcgcgatcc gcagctgaca ttgaaggaat tgaatcgcgt
      481 ggcaggaaaa ccgggagtgc gcgcggtaca tttgccggat tcggtggagc ggcacgatta
      541 catcgtcgat cccaatttcg agcccgttct cgcgcgcatc gaagaattag gctacccgat
      601 cgtgtttcac cagatggatg gcgaagccaa cacctacggc ggcccacgtg tgatgggcaa
      661 aatcaatctt gcggccggac tcgatgcgcc gatcgatcac accgttctcg caacaaaact
      721 aatgatggcc gggacggttg acaaatatcc aaaactggaa atgatcttgc cgcacgcggg
      781 cggagccttt ccgtatctag cgggaagaat cgaacacttt ttctaccact ttaacgggca
      841 gcaagtgact ctcgcgcacc cgttccgcga ctatctgcgg cgtttttatt acgactattt
      901 aatttattat ccggaagcgt ttcagtttct cgtgaccatg gtggggacag accgcatcgt
      961 agtgggcacg gatatttttg ccgcgcagga cgtgcagtat cccgcagaag ttctcgacca
     1021 gttcaaactt cccgcccccg aacgcgagcg catcctgaaa ggaaacgcga tcaagcttct
     1081 gcatctttag ccgtaagtag cacaggcttc agcctggggg ttttagcccc tgcaaggact
     1141 aaattccaca ggctgaagcc tgtgctaccc aattccattt ccctattttc cttttgccat
     1201 gttaaatttc tgcacgcgct ttcccgtacc cacttcgccc acgtaaatgt tccccttgga
     1261 atccaccgcc agaaaatggg cgtaggttaa ttgcccaggc tgccgtccga cgcgtgcgaa
     1321 actggagagc aatttccccg tggcatggtc cagaatatcg atcttctcgt tgtcctggtt
     1381 gaccacatac atatatttct gtcccgcatc ctgcgaaaat cccaccgaca ccgtcgatcc
     1441 taaaattccg gattcgtgcc ccgggccggg cggcaaactg gaggtcgctg tgaatgcaac
     1501 gggaatgttt ttctgaaact cgccagtttt gtcaaagacc tgcacacgat ggccacgcct
     1561 gtcacagaca taaacaagtc ctgcgttgtc catcgtcacg cagtgaagaa ttggcacaaa
     1621 agaatttcca gtctcgctcg ccgtgcgatt caattcccac tgtcgcagga attttccgtc
     1681 tttatcgaaa accgcgatgc ggtgattgcc gcctacagtt tctccatcgg ccacatacac
     1741 gtcgccgtct ttcggatcaa tcgaaatgcc cgcgggcttg aagaaggagc tttgccctgc
     1801 gttgagaggc ttccctttcg ccgtgccgtc ggacgagtcg aacattccca tcttgccgat
     1861 ctgcaacaga agcttgctgc cgtcgtgcgt atactcctgc acgatgccgt cgcccgttcc
     1921 ggtaatccac aaatgatctt ccttgtctaa tgtgcacccg tgcggcgtgt caggagtggt
     1981 ggaaggatcc ccaaaagaat tcacgacgtt tcccgccgga tcgaactcaa tcaccacggg
     2041 cgcctgccgg ccagcgtcca gatcattgtc cgtcaaatcg cgccggttca aaataaaaac
     2101 gtggtcgtgt gagtcgatgc acaagccgcc gacgccgccc gtcacccaca gattgggtag
     2161 cggcttcggc cacatgggat cgacttcaaa cttgggcgcg ctttcctgcg cgcgagcaga
     2221 gcccacccaa accgctccgc cggcaattgt caaacccgcg cacattgcgg cgatacgaat
     2281 aaaatcggtc ttcattttca tgagattcct tgactgctga ttgaaggcgt actacggacc
     2341 tttgccgtag cccgctcctt cagaggcggg gccacagcaa ctataactgc gtgcgataaa
     2401 catttccgta taacaaatta catgtgatct ttcatcacaa aaggtgtgtg cggcttactg
     2461 tgaataaacg tcgacacatc gtacgcctcc tgcggcgtca gcgtatccgg cgcattctgc
     2521 ggcatattgt gttgcacaaa cgccgccatt tttgaaattt gattcatgcc cgcaccgtcg
     2581 ctgaacgagt tcggtcccca cagcgcaggc gccaccggca atttccccgc accgtcggcg
     2641 ccgtggcaga cagcgcactg ctcgccaaaa attttcccgc cattcacggg atcgccgttc
     2701 aattcaggca gtgtaacaag cccccgccca ggaaacggct tgcctgtaac ttgatcccgg
     2761 gaaagccatt gcgagtacgc gacgagtccc gtcatctccg ggccatcatt cggaacgcgg
     2821 tgccccgatt cgctgcgttg aaaacactgc tcgatgcgcg cttcaaatgt aatcaccgtt
     2881 ttttcgcggt cgcggtacat cggaaatatt ccgggcagtc cgaccatggg catcgcttgc
     2941 gaaaccgtgc caccatccac atggcaatgg gtacaactca acttattccc cacatatttt
     3001 tccgcatatt tcggcgtatc gttgaagacg ttcatcccga gccggatgct gtcgcccagc
     3061 gggcccgcgg gaatcgtcgc aggatcgggc gcgacccatt ttttggaagt atccgtggcc
     3121 tggggagtcc tgggcgcgcc atagctaacc gggatgaacg cgagcacgct aagcgcggac
     3181 accgccagcg cgacaaataa accgctgcgg atggcgatcg ttcgattcat caatgtcctc
     3241 ccaaggcagg gcagttttgc gataccgtac ttaatattct accgcttcat ggtttccgga
     3301 gtgtagtgcg ttcctaaata ctgaatcatc ttttgcgctg tgctgtcaga aacggacaag
     3361 ccgtaaaatc ctctcatcat gttcacttcg tgttcccatg cctgcgcaga catgggcggt
     3421 cgagtggtga tgtaagccag ggcgtggcac agcccgcaat tgtaggcaac ttcattcttc
     3481 ccgtctcctt cggcaagatc cggtgaatag gctggattcg ggccaacatt ataggcagct
     3541 tgcgcgggag ccgctgtcgg cggcgcctga tggacggact gtgggctagc ggatttatca
     3601 cttccagcat tgaaatccgc atagataccc tggccaacca acacggccag catccctaca
     3661 gccgaaatga aaacccactt ccgtttcccc atgttcccgt gccgcgctta tagttacatg
     3721 cccgctggtc cgcaaccgca caaatcatac tctcgccggt tgcggaccgt ccactttggg
     3781 taatttcgga gtttataaac gcatcgtgta aacctgcccg cactaaattc gtgcgacctc
     3841 tacgaaaacc aaagcgcatg caaactcatt ctcttcgagg ctccgccatt gaaattgtga
     3901 tatcttttct ctcatccacg gaggtcgccc atgaacgtcc gaacgctatc cagcgccgca
     3961 acctgcgcca ttctgctctt ctcgtgctcc ctgtttgccc agacaggtgc caaggaaacc
     4021 agggtgatga tccgcgcggt agcgcgcgat gccaaggtaa tcgggacgca cgtaggcggc
     4081 gcaaaaatta cggtgaagga cgcagccacg ggagaaatcc tcgcgcaagg tgtgcagcaa
     4141 ggtggcacag gtgacacgaa cacaattatg aaaactccgc gcacgcgcgg catgagcgtc
     4201 ttcagctccg ccgacgcgtc gggctacctc gcggtgctgc acatcgataa gccaaccgtc
     4261 gtggaaattt ctgccgaagg tccgttgggt aactcgcagg ccactcagcg cacatcgaaa
     4321 acgatgctgc tcgtgcctgg agaagatgtt ctcggcgaag gaattttgct ggaaattcat
     4381 ggcttcatcg tcaccgcggt ttcaccgctg gcggatgcaa aagtgaaagc cggtgcgccg
     4441 ttcgaagtga aggcaaccgt cacaatggct tgcggctgcc cgacggagcc cgacggcctg
     4501 tgggacgcaa acaagattca cgtggtcgcg cgtcttcttc gtgacggaaa aatacaatcg
     4561 gaaattccaa tgacctacgc cggtgtccag aacacgtttc acgccgatgt ccccgccacc
     4621 gccgccggac ctatggaatt gcaggtcctg tccatggacg cggccaacgc caacttcgga
     4681 atattgaaag aagccgtcac catcgttccg taactgagag cctaggaatt tcaaccgcta
     4741 ctccccaaca cgacagctac ccttcccggt gaaaatagtt gtattctgcc ctcacaccaa
     4801 tattgcgaag caagcttccc gggagactgc atggaacgcg cgctaaccgt cagtgaaatg
     4861 atcgaccagc gcccgctgag ccgctatcag atctgggcaa tggcgttgtg cgcgacggtc
     4921 gtagtgctcg atggtttcga cacgcagagc atcggcttcc tcgcgccgtc gatggctgaa
     4981 actttacaca tacccattaa aactttcggc cccgtttttg gcgcggctct gtttggcctg
     5041 atgatttcgt ccatcgtaat cggtccgctt gcggaccgct ggggccgcaa gtggcccatc
     5101 gtgatttcca cactgatttt cggcgccttc gccatgctca cgccgcgcgc gacatcgctg
     5161 cccgaattgt gggccgtccg gttcgtcact ggcctgggat taggcggcgc gatcactaac
     5221 gtcctggcgc tgatgtccga atattcgccc aaacgtcatg tcgctgtatt ggtctccatt
     5281 ttattttgcg gcatgccggc cggcgccctt ctcggcggat tgatcagttc cttcatgctg
     5341 ccgcgatggg gttggcagtc cgtgttttac gccggaggaa ttttaccctt cctcctggcc
     5401 ttgttgctga ttgcaattct ccctgagtcc gtccgctacc tggaaatttc gggcgcggat
     5461 cccagccgaa tttcaaaaat tctggcgcgc atttcgcccg atcttggcac ggccccgttc
     5521 tctcgctcgc cactacaaac ccagcatcgc aacgcgcccg ccgtccgctt gttcaccgaa
     5581 ggtcgcgcgg tgggaactct cctgctctgg gttccctact tcatgaatct gctgatccta
     5641 tatttcgttg tgagctggct gccggcgctt cttcggcaaa aaggttctcc ggtgtcagcg
     5701 ggaatcacgg caattatttt attcagcgtt ggcggcatcg caggctccgc gaccgaaggt
     5761 ttcctgatca accggtggag cgcgttcaca attttattgt ctgaattcct ctgcagcgcg
     5821 gcgctaatcg gctcgctcgc ctactccacg tccctaccgc tcacgatgac aatcaccctc
     5881 gtgctgggct ttaccgtgca ggccgcgcaa ggcgggctga acgtgatggc cgcaacgatt
     5941 tacccgactt ccattcgtgc cacggggatc ggttgggcgc ttggtatagg ccgcgtcggg
     6001 tccatagtcg gcccggtggt cgttggcgta atgctcaagc tgaattgggg tccgcgggaa
     6061 atttttcagg caggcgcaat tcctgcattg tgcgcggccg cggcaaccct gctgggcctg
     6121 tggcaaagga gagatgcatc cacgggtccg cacactcctg aggccatgaa gcaaccctcg
     6181 ctgtcgcatt gagcatggat tggaacgcga tacaacgcct gaagtacgcc tatggtgttg
     6241 cgctttgcca cagttttcta aactgctcga tcgtccccgg tccgtaaccc gcgtaatgat
     6301 tgttggcata cgcctgtatc ggaattctcc gctcgcgaac ttttcgcaac acgtccaccc
     6361 actcgccgag ttccgccgtg cgatccaaaa tagttttgtc ccaaactttg gtctgcagct
     6421 cgatgccctt acgatcgccg agccatcgca catatgcgaa gtccgaggtg atcggatcta
     6481 ttcgcgcaaa ttgctgtttg ggcccaggca tccacgactg atcgataagc gctagcgcga
     6541 cgttgtgttc ccgcagcacg tcgacaaatg ctggcacgag ccagtactta ttccgaatct
     6601 ccaaggcaaa gcgatactcc cgcggaattt ttttaagaaa tggcacaagc cgcgccagga
     6661 agtcttgaac tccggaaaac gcttgcttgt tgaagtagcc gaactgtaaa agcatcggac
     6721 ccagtttttc gccgagatta tccatggctc tgagaaaccc atgaaactcc tcgtcgcaat
     6781 ccgccagcat tttttcgtgg gtgaccaatt gcggaacttt cgccgcgaat aaaaatccgg
     6841 gcggggtctt cgcataccag cccttcacgg tggaaatcgc gggcgtacga taaaacgtgc
     6901 tatccacttc caccgtatcg aatttcgtgg cgtagtagct caaaaaatcg gcgggtttca
     6961 ttcccgcggg ataaaacgtg ccctcccacc ccgccgcggt gaaggccgaa gtgcccagtc
     7021 gaatgtcagg agtgtccacg gtccgcgatt ttaactcgaa ttcctgtgcg cgggaagaaa
     7081 ccccgcccct taacatttac tgacgccgct cactcctggc aacggctgcc tttccatacc
     7141 tgctcaaaaa atgtctgcgc gctggcatcc accggaaggt cgccaacgta aacaggctgg
     7201 tcgcctttta aatccacgac cagcactttc ccgctgtgat tatgaaacgt aaaccgcagc
     7261 gctggctctc cgtccgcggc gtccacggca acaaaatgtc cgtcctggca atggaatctt
     7321 ccgccaaacg catatccgga aaacgctttc gcggatttat tcaaaggcat gtcgccctct
     7381 agctggtaga tcaggctgtc acctttccag ttcagcgttc cagcagagct ggcattcagg
     7441 cgaaacgtca aggactcaac tattttgggt tgcgacggtc ctttcgcctt gtccggaaat
     7501 tccacatacg gcgtcgtgac caccggcgcg ggtggcgcga cacgtacaga cgccttcgca
     7561 atttcggacg gcagcacggg aatatcaatc gcgccagtca atcccgaatc tacatccagc
     7621 accacgaggc gaattcgacg cgtgtccgcg gaaggcatca gcgcgatgtg atctggaatt
     7681 ccggacgctt gccagccccg gaacacttcg cttgtaactg tttttggcag ctctctctcg
     7741 ttgaaacgaa acaccgtcgg cttctgcgcg aattcgcaat cggccaggcg aaggttcatg
     7801 ttgtaggaat cgcccgcctg cgtggcgctc aacccagcgg cggagatcag gagcatatac
     7861 tgcaaacccg ctggcgcgtt tccgttcgga ccagcggtca tcggctgcac agtcagtgga
     7921 atggttgtcg agggcaattc gtcctcgcaa gcttggcgaa gatgctcctg cggcggctgc
     7981 cgtttcgcga gggaagccgc gtcgagtgcg tagtagccgc gacggtagac aagcttcact
     8041 cccggccgcg aagttttcac cacaatctta tggaaccttc cgtcccagtt tgccttcgac
     8101 ggataaaatg acatttcgta atacgacgaa ctgtctttca gcgccgtcat gaggcagccc
     8161 gcaagatcat tgctgttctt gcaggacttc ccgcccgtgt cctgcgcaat ttcatccatc
     8221 gtgcccagcg tttgttggaa atccgtgtcc tcaacactta gacggttcgc aattgcgggt
     8281 cccgtaagcc gcgtctccgg aagattgatg ttctgcgaag ccgagaggga ttgctgcgcc
     8341 tgaagtccac gaacatccat cggatatacg gccacttgcg catccgtcaa ttgatttccc
     8401 acaattttga tttcttccga atattcccgg cttccgccaa atggattgtt tcccgtacca
     8461 ggatcgggga cgatggtcag cgggaaggat tccgaaaccc agatcagatt tttgcgaccc
     8521 ggaattccgc tgagatattg cccgatttga gaaagcgcgc cgagagtctc ctttacccgc
     8581 aggtccacgg acaaggaata ttcttccttc tcaaaatttt gaacttcgcc tagctgcgcc
     8641 tgtacaccca tgtttacgtc catgccggcg gtctgatcct gcacgtaatt ggaagtgtcg
     8701 ctgaggttat tgcgcggatc gggttgaatc gaattcccca gcaaagttcg atccaacgcg
     8761 gcacgcaaga gacgtccatc gctcgtaaaa ccctgcaata ttctcagcga acttcccagc
     8821 agaaaaacgg cgaccggcgt atccggtgga aggcttctca ttaattgcag cattttggcg
     8881 cgaccctgca tctggttcgg cgtctgggtg ttcaggctgt ccatcagtaa aacggtgggt
     8941 ggaattctca gttggtcaag cggcaactga ttggaaatga tgccggcggc tttacgtgca
     9001 gcgctcgcgg aattttccga ggttccagga cggttcagcg tttccagaaa ttcaaaatgc
     9061 tcaattttct gttgcgcttt gcgatcttca aagatttgca gttcttcagg cctgaggtcg
     9121 cgaatcgcat taccgtgcga gtcggttgca accacatcga gcgtcacgag ccgcgtcgtc
     9181 acttttaaaa cgtagccgga ttctcccttg tcggactgtt tcgcttcctt ttgagccgaa
     9241 ggggcgccgg gagtggcgga ttgtgacagc gattgacccg cgctcaagcc agcagaaata
     9301 aagcaaacgc ttaaaattgc aactggccac ttcggacgca tggaacctcc gccttaggaa
     9361 tcggtacacg ttgggtccgg atgagtgatc ttacctgacg tgttggagga aacgctacga
     9421 ttccctgaaa tgccgcgcgg aacaatccat tgccgctggc aaagcgcaat aaatcgtggc
     9481 acactatgcg catggcaaac aaaaacgcac caaaacgcga aaagaaaaaa cccaagaaag
     9541 ccaagaagta gtagtccaac atcaattgac acgtgacccg ccgcgatttt tcttctcacg
     9601 cgaggagaga tcgcggcgct cacgcgtgaa cccttcaacc gaaactttgc ttactttttt
     9661 gctgatgatc ggtagaatac ccgtcgcgcc aacggtgcgt acactccgat tcagcgaaat
     9721 gcactctccc tccttccgct tttctgcaat tcagttcgca caagaattcc tgcgattcgt
     9781 ccgcgagttt cggcagcaat tccccgggaa gaattccttc cggcccgact tctccgcgtt
     9841 tctgctccac gtgctgcaag atgcccgcaa tcccggcctc cgcaaattca tctaggttac
     9901 tggccgctga gggccagaaa ttttgcgcgc tcgagcaacg agttgctgac gtctgatgct
     9961 ccttccaggt gccgtttgtc ccttcgtaac cgtgattttg cgagcctttt gcgatacaac
    10021 tctgaaatcc ctcacactca tcaaaccagc attagagttc aggacgattt tccttcaggt
    10081 tccgcagcaa tcggggctgc gaacgtgaac ggacgaggag catggacatg agccccaagg
    10141 tacaagggaa gacgagcagg ataccaagta agtttttgat tataattggc ttgggtattg
    10201 ccttgagcgc ggtgtcaata gccgctcgag cgcagcagga tcagccaaac aattatccaa
    10261 acaacggcgc agacacccgg gagcaacgga acgacgcgcc tcctccggac agccagatgc
    10321 agccgccaga cccgaacgat cagcagcaac agaacttaca gcaggaccag gtacccgcgc
    10381 cgcaggacca ggtacaatcg agccagccag ttccgcccga aactttaacg attcctgccg
    10441 gcactctcgt ccgggtccgc gtcgatgaat ggatttccac cgatcgaaac gtgaccggcg
    10501 atagttttag tgccaatctc gaccaacctc tggtcgtaaa tggctgggta gtggcgcgtc
    10561 ggggccaggc cgaaacaggc cgcgtgtcac tcgtgaaaaa gggcggccac ggaaatggaa
    10621 gttctcaact gggactcgat ctgcccgact tgacattagt cgatgggcag caattgccat
    10681 tgcagacgga attgttccag acctccgccg gctcttccgc gggtaggaat gccgcgaccg
    10741 taggcacgac aacaggaatc ggcgctctga tcggcgcggt tgccggaggc ggcactggcg
    10801 cggcaattgg cgcaggtatt ggtgctggcg cgggcatcgc tggcgtaatg tccacgcccg
    10861 gcaaaccgac cgtagtttcg ccggaaactg tgttgtcctt ccggctgcgg aatcccgtga
    10921 cgatctcgac gaccaacagc cagtttgcct ttcagccggt atcccaatcc gacctggatt
    10981 ctcgcccacg agaaaaccga ccgcagatga ggcggcccgt accaccgcca gactacggtt
    11041 atccctatgc ctacgggtat cccgggtacg gctatggcta tccctacggt tggtatccgg
    11101 cccctgtgat cggctttgga tattatggtg gatacggcag gttccgcgga ttccgtcgct
    11161 agcctggctt ttctgcatga gaaatgacgg ggcttgggag tgctcttaaa gatctttagc
    11221 gttcccaagc ctcactttgt tggggcgccg gattctcttt ccaccgcgac cgcaatactt
    11281 ctccacgccc gcaacgtttc acccacggtc caggcctgag caacacatcc gcggggaaaa
    11341 tgcggcggat cgccatcgaa aatttcgctt agcgttccca gacccccgga tttaatttcg
    11401 ctcagcaacg gttcgagaaa agacatggcc tgccgcgcgt ctccatgcac gcgcaagtgc
    11461 gaaagcgcga acggaccgag cagccaaccc cacacggtgc cttgatgata cgcatcgtcg
    11521 cgctcttccg ccgtgccgcc gtaatgtccg cgataccctg gctcatctgg cccgagactt
    11581 cgcaggccga acgatgtcag aagttcgcga gcgcagactt ccaccacggc gcgctgctgg
    11641 tccgcattca aagcggtttc cggcagcgaa acagcaaaaa tttgattcgg acgcagcgtc
    11701 gcatcttttc cgtccgggct ccccggagca tcaatcacat cgaagcaata ttgtttttcc
    11761 gcgttccaga attttgcaaa gcccgcacgc gcgttatttt ttattgtttc gtacctcgcc
    11821 gcatcactgc ccacgatctt tgcaaatcgc gccatcgtcg ccgccgcgtt cagccatagc
    11881 gcgttgactt ctacgggctt cccgatgcgc ggagtcacca ctcgcccctt caccttcgca
    11941 tccatccaag tcagctgcac gccaggctcc cctgcaaata gcaacccatc cgcgggatcc
    12001 acgtgaatgt ggaatcgcgt cccgcgcacg tgcgcctcaa taatttcttc gagcgcagga
    12061 tacaattcgt gcagcaggcc aatatcgctt gtcgattcaa aatactgcct taccgccttg
    12121 aagtaccaca gtgtcgcatc cacactgtta taggatggcg ccacccccgc ggccggaagc
    12181 tggttgggca gcatgccctc gctgacgaag tgggagaaag ttcgcagaat attccgcgcg
    12241 agccacggtc gtcccgtcac caggcataac ccaggcaacg ctaccatcgt gtcgcgtccc
    12301 cagtcgttga accacgggta accgcccaaa atagtttttg cgttcggcgc gtcggccagt
    12361 gggcgcgccg caataaattg gtccgcggca agaacgagct gcgacacagc gggcggcacg
    12421 ctctgcgaat tcccttttgt gcccgcgaca ttcgccgcgg aaaattttgc cagtaaggca
    12481 cgttcctctt tctgtcgttt cgcaaaagcc gcttcaccgt ccagcgatgc cgctgcttcc
    12541 gtactcgcga caatcgtcaa tgacgctccc ggtgcgatgg ttgcgcgaag ctctcccgcg
    12601 aaaaaatggt cggtgacgtc cggaagccct cgctcatgct ccgccgaaag atcgaaattg
    12661 cggtaccagt tgctcgcaag acttgccgcg gccgtgtcgc tcaccacgaa aaatggcgtc
    12721 gcgccttcga acgccactac ctttaaccca tgctcaatgt ttgttacgga catcggatgc
    12781 gtgtgaccga ccgtgacgct gtgctcgtcg gcatagtcag ccagcagttt tattgaaatt
    12841 tcaaccggcg aaccggcgcg caccacgcga tacagcgcat agctcgtgtt ttccccggac
    12901 gccataaaga tccgcttctc aatgagcgcg tcggccagcg caaatgtcca gacgggagtt
    12961 gtaccttcca gatgaaatcg ttcaatattc agaaaaccct gaggagcgag cgtcccgtca
    13021 gcccagcgat ttgtggagag cgcaaaattc ttgaaagcat atcgcgcagt ttcatcgagt
    13081 ttcgcgacga gtaaggtgcg acccgacggc ggctgcaccg cggccatcag aaaaccgtga
    13141 taacagcgag tttggagccc tgcaacggtg ccaaatgcgt acccgccgat cccgttggtc
    13201 accagccact cgcgcgtttc tgaatcgtgg aggtcgccgg acatctcgcg gtcaaattca
    13261 acggtaacag ggctgaaggg cgccggagga ttgtagttgc gcgtcacaat gaaatcatga
    13321 agccacttgg caggtttcgg ccagaaaaaa attccagcat gcgcagcgaa tcacatcact
    13381 taacaatttt gctgtctgaa aatatgtgca agccaacgag gtaggtgcgc agcgatccga
    13441 gatcgatggg cttcgtgaaa caggtatcac aacccgcttc ttccactctt ttaaattcct
    13501 taaacgaggc aaaagaagcc atcgcagcaa taggcaagcc cgcaaggtaa ttctgccggc
    13561 gcacccagtg gacgagggta agtccgctgt gaattcccaa ttgaatatcc aacagcacaa
    13621 cgctcggcat cggagggtcc gcaattttta cgaaaccctc ttccagcgat tccgcaagca
    13681 ccgcctcgtg gccttccgac tccagccact tacggagcga ttcgcgagat tgcgcatcgt
    13741 cgtccactac cagtatccga tgcttcactt attttccctc aatctgaaat ggatcctagt
    13801 taaccggagt cgggcgccgg acgacacaat gcacgatcca ttcatcgtca acatcgaatt
    13861 aaggcttgag tggagcttgc gcgacgaacc tgtccggctt ttcaagtttc aacgtggaac
    13921 ggaacggggc cttatttcaa actttttcgc gcaacggttg cgggttcttc attctccgcg
    13981 tcggattcca gcttggacgc ccattgcgat aaatggtcca gcgaaatttc gtgcgtgcga
    14041 tccagatgca gaggcgtgat ggacgctgcc ccgtcgaaaa ccgcagcgta atcactttga
    14101 gggtcaagtc cttcgatatg ctcttgctcg agcaaccaga aaaaattgcg cccgcgcgga
    14161 tccgtgcctt cctgcaaaac gttgcgcgtc accttcttcg attgccgcgt catgcgcacg
    14221 ttgccattcc ataattgagg tacgtttaca ttcagcatcg ttcccagcgg cataccttcg
    14281 acaagcgcca tttgcgccag atcacgcgcg agttttgccg cctgatcgaa tcgaaaaact
    14341 cttcccctgt gcactgcgga cacggcaatc gccggaatat gattgatcgc tccttccatc
    14401 gccgcgccga ccgtgccgga ataaaaaatg ttctcgccca aattaccgcc gcgattcacg
    14461 cccgatatca caatgtccgg ccgctgcggc aacaatttgt tcagcgccag aatcacgcaa
    14521 tctgcaggcg tgccctccac agaccactca tgctccgcca cctgatccca aaatattgga
    14581 tgccgcagcg tcaacgattg cgccgcagcg ctccgctcat gagccggcgc taccacgctg
    14641 atcgtgccaa gcccctgcac cgcctgcacc agcgcgcgaa gcccttccgc ctgaatcccg
    14701 tcatcgttcg taatgagaat gtgcgccatg cttaagtagg ataggcttca gcctgtccgg
    14761 tttagattaa gttagaccca acctgtctgt atagaagtgc gctaaacatt cgaccgcacg
    14821 aaatcaaaaa aatcgcagcc cggtaaaccc agtcgctcga ggccctaatg ttaacaatca
    14881 acgaagcagc tcaacaagct gcagataatc gaagtttcca acgcggcgat ttgttgcgac
    14941 cttgaggctt tagaacgaaa gaagaaaaat ggtcgggacg actggatttg aaccagcgac
    15001 ctcacgcacc ccaagcgtgc gcgctaccag gctgcgctac gtcccgactg tctgcagggt
    15061 aaatccgatc tgtagcccgc ctctttagga gcgaagattt agacgccaca tagccaaaat
    15121 ccccgccgct aatccctggt tttcactcaa gataagcggg gcatttacag caaaattcta
    15181 acgagccagc gtatcactcg gattccagag ccgtcaacaa cgagcgcagc gaatcccgca
    15241 aatccagcag cgtctgacgg ttaaatacag cgaggtgcgg cgacagcgct ggcgcagcct
    15301 gaggcggggc gtgctcttcg ccgtgattcg catgcatggc gggcgtggcg tttgcaagca
    15361 atccgccgtt cgccccttcg ccggcacccg ttccaagagc ctgatcgcgc aaatgcctcc
    15421 gcgcgcccgc aatcgtaaat ccttcgttgt acagcaaccg cttgatggtg aagacggtgt
    15481 cgatatcttc ctgcctgtac agccggtgcc ccttggggct tttcaccggc tgcagcatcg
    15541 gaaactccgt ctcccaatag cgcagcacaa acggcttcgt agccgtcacc cggctaacct
    15601 cgccgatccg gaataattct ttccccggaa ttgcggcctc gagagcgcca ccgtgcccat
    15661 taccgctgcc gttgttttcg agttcttcca tgtggccact cacccttgca aacttatagc
    15721 cctaatccta atccgttttc agccagtgtc aacgccccag tgaaacactc ccgcggtttc
    15781 ggacggcaac gaaaacctta gctgtcattc cgagcgagcc gaggaatctt gctttctcat
    15841 cacgctggca aaccgaagag aaatttctcg cttcgctcgg aatgacagag aggatgcctt
    15901 caactaaatc acgaacgcac cttggcccct actcgtcgcg ctagcgaatg cgaatccgca
    15961 ccctattcct ccacaggctc ttctttgcgc ggcgtccgct tcacgcggtt atggctcatg
    16021 ccaggatgct tcggcacggg agtgcgccgc gtcgatgact tcgtccaatc gcgcttttcc
    16081 ttcatccgct gcaaaaaacg aatcggcgtc ccttcaaaat caaaactctt gcgcagcaga
    16141 ttttccagga accgctc
//
LOCUS       DASZMB010000205         4578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2954311, whole genome shotgun sequence.
ACCESSION   DASZMB010000205 DASZMB010000000
VERSION     DASZMB010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2954311"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /gene="hpt"
                     /locus_tag="VG272_09345"
     CDS             complement(<1..543)
                     /gene="hpt"
                     /locus_tag="VG272_09345"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HWF13924.1"
                     /translation="MALHGHGQEHLKVLLRSSAIQKRVREIGLQISKDYKGQRVHLIG
                     VLKGAAIFLSDLVRQVKVEVSLDFMSVSSYGHGSQTSGQVRLNKDLDTSIEGLNVIVV
                     EDILDTGLTLGYLLRVLQQRKPNSLKIAALLDKPERRIKKDVKADYIGFQIPNEFVVG
                     YGLDYAERYRNLKDVCVLSLP"
     gene            663..1679
                     /gene="rfaD"
                     /locus_tag="VG272_09350"
     CDS             663..1679
                     /gene="rfaD"
                     /locus_tag="VG272_09350"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR02197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HWF13925.1"
                     /translation="MATMNLVNESWKDKRVLVTGAAGFIGSVLVWELNRRGCKQVLIT
                     DFPAGGDKSANLAALRYTEFIDASDLHACLASGSLGKLDCIFHLGACSSTTETNEQYL
                     RENNFEYSRTLAEWALAASVRFVYASSAATYGDGSAGMDDSDLRHMQRLKPLNLYGLS
                     KQWMDLHALKEGWLDRIVGLKYFNVFGPNENHKGEMRSVVCKSYGDVLTTGVIRLFKS
                     YRTEYHDGEQRRDFLYVKDAVAMTLHLAENPAANGIFNIGSGQSRSWNDLARAVFSAL
                     GREPHIEYIEMPEAIRDKYQYFTQANIGKLLATGYDECITSLEDAVRDYVVNYLVPGK
                     TIGS"
     gene            complement(1761..3095)
                     /locus_tag="VG272_09355"
     CDS             complement(1761..3095)
                     /locus_tag="VG272_09355"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HWF13926.1"
                     /translation="MKLPPGPKGFPVLGSVFEPKGDTITYLKKCTREYGDIVFFRFMG
                     VPACYLSRPDYIESVLVTQNNNFVKSKDYRAMRRVLGNGLLLSEGEFWRRQRKLIQPA
                     FHQEKITAYAQIMVDYTQRMLETWSDGQTVDMHEAMMRLTLGIVAKTLFDADVSHEAE
                     DVDAALAVLMGKFLRQAGMALLLPTWVPLPTSQLLRRAVGRLDKVIYRIIEQRRTSGQ
                     MSGDLLSAFLQTRDDEGVGMTDRQLHDEIMTLFLAGHETTANVLSWTWLLLGQNPETE
                     AKLTEELERVLHGKIPTPADLPRLVYTDMVMRESMRLYPPVWVIGRRALTTFRLGEYE
                     IPANTNMLISQYLMHRDSRYFPEPEKFDPERWRAGDPRATALPRFAYFPFGGGPRVCI
                     GAAFGMMEAVLLLATIAQKFKIEVSPAEKVSMQPSVTLRPRNGIPVKLKRRF"
     gene            complement(3101..4225)
                     /locus_tag="VG272_09360"
     CDS             complement(3101..4225)
                     /locus_tag="VG272_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HWF13927.1"
                     /translation="MYRSDVIGSLLRPAYLKKAREDFAAQRLTDAEFKCTEDRAVDES
                     VALQLRAGLDVLTDGEMRRYAFYGHMIDAFEGFDKFGGWSIPFRDDKGEVTNHPRPVV
                     VSKLRRKWHMCAEEFTYLRARTDHTAKTTLLSAQQAAAYYDSKKSSGAYPTVDSFLAD
                     LVDLLRGEVAELVRLGCTYIQVDSPQYAALIDANIREGYRQRGNDPDRLLDLSIEMDN
                     AVIGGHPDVTFGLHLCRGNNQSKFYASGDYAPIAKVFRNTKFQRFLLEFDDERSGGFE
                     PLSHVPSDRTVVLGLISTKKAPLEAKDEIKSRIHAAEKFVPLERLALSPQCGFASTLE
                     GNLLSEADQEAKLRRVAEIAREVWGDAKSPVSSKVKGKSV"
BASE COUNT         1017 a         1217 c         1280 g         1064 t
ORIGIN      
        1 tggaagcgaa aggacgcaga cgtccttcaa attgcgatag cgctccgcgt agtcgaggcc
       61 gtatcccacg acaaattcat ttggaatctg aaagccaatg taatcggcct tcacgtcttt
      121 cttgatgcgg cgttccggct tatccaagag cgcggcaatt ttcagcgaat ttggcttgcg
      181 ctgctgcaag acacggagca ggtagccgag cgtcaggccc gtgtccagaa tatcttccac
      241 gacgatcacg ttgagcccct cgatgctggt gtccaaatct ttgttcaggc gcacctgccc
      301 ggaggtctga ctgccgtgac cgtagctgga tacggacatg aaatcgagcg acacttccac
      361 tttcacctgg cgcaccagat ccgatagaaa aatggcagcg cccttcagca caccgatcag
      421 gtgcacgcgc tggcctttgt aatctttgga aatttgtagc ccgatttcgc gaacgcgctt
      481 ttgaatcgcg ctgctgcgca acaaaacttt caggtgctct tgtccgtgtc cgtgcaaagc
      541 catagacctc ctcgagagcg gttactctag cgcagcgagt gggaaagctc aagacgtgga
      601 aagcttgcaa aggtcgattt gtcagaccgg attgattcgc gtggcgcgcc atggcaacct
      661 tgatggccac tatgaacctg gtgaatgaaa gctggaaaga taaacgcgtc cttgtgacgg
      721 gcgctgcggg atttatcggc agcgtgctcg tgtgggaact gaatcggcgc gggtgtaagc
      781 aggtgctgat tacggatttt ccagctggcg gcgacaaaag cgccaacctc gcggccttgc
      841 gatatacaga atttatcgac gcttcggact tgcacgcttg cctcgcttcg ggatcgctgg
      901 gaaagctgga ttgcattttt catttggggg cctgctcttc tacgacggaa accaatgaac
      961 aatatttgcg cgaaaataat tttgaatatt cacgcacgct tgcggagtgg gccctcgcag
     1021 cgagcgtgcg attcgtctac gcatcgtcgg cggcgaccta cggagatggc agcgcgggga
     1081 tggacgattc cgatttgcgg catatgcagc gtctgaaacc gctcaatctt tacggactat
     1141 ccaagcagtg gatggacttg catgcgctca aggaagggtg gctcgaccgg attgtgggct
     1201 tgaaatattt caatgtgttt gggccgaacg aaaaccataa aggcgagatg cgcagcgttg
     1261 tctgcaaaag ttatggtgac gtactaacta cgggtgtcat tcgtctattc aaaagctatc
     1321 ggactgaata tcacgatggc gagcagcgac gcgactttct gtatgtgaaa gacgcagtgg
     1381 cgatgacgtt gcacctggcg gaaaatccgg ctgctaacgg aatttttaat attggctcgg
     1441 gccagtcgcg ttcatggaac gatttggcgc gtgcggtctt ttccgcactc ggacgcgagc
     1501 cacacatcga gtacatcgaa atgccggaag cgattcgcga caagtaccag tattttacgc
     1561 aggcgaacat tggaaagctt cttgccacag gctacgatga atgtatcact tcacttgaag
     1621 acgctgtgcg ggattatgtg gtgaattacc tcgtacctgg aaagacgatt ggatcttagt
     1681 aggacaggcg tcagcctgtc tgggttagat tttacagcct ttgcgaaagt caaaagcaga
     1741 caggctgaag cctgtcctac tcaaaatctc cgcttcagtt tcacggggat accatttctg
     1801 gggcgcaggg ttacggaagg ctgcatactg actttttccg ctggcgacac ttcgatttta
     1861 aatttttggg ctatggtggc taggagcagg actgcttcca tcatgccgaa agcggctcca
     1921 atgcagactc tcgggccccc gccaaaagga aaataggcga agcggggcaa cgctgtcgca
     1981 cgcggatcgc ctgctctcca gcgctccgga tcgaattttt ccggctctgg aaaataacgc
     2041 gagtcacggt gcatcaggta ttgcgagatc agcatattag tattcgccgg gatttcgtat
     2101 tcgcccaaac gaaatgtggt aagcgcgcgg cgtccgatta cccacacggg cggatacaag
     2161 cgcatcgatt cgcgcatcac catgtccgtg tagacgaggc gcggcaaatc ggcgggcgtg
     2221 ggaatttttc cgtggaggac acgctcgagt tcttccgtaa gtttcgcttc cgtttccgga
     2281 ttctggccta gcagcagcca tgtccaggac agcacgttcg ccgtagtctc atgccctgcc
     2341 agaaacagtg tcataatttc gtcgtgcagc tggcgatccg tcatgccgac gccttcgtca
     2401 tcacgcgttt gcaaaaatgc gctgagcaag tctccggaca tttgtccgct tgtgcggcgc
     2461 tgctcgatga ttctgtagat cactttatcg agcctgccga cggctcttct taaaagctgc
     2521 gacgtgggga gcggaaccca ggtgggcaaa agcaacgcca tgcctgcctg gcgcagaaat
     2581 tttcccatca gcacggcgag cgcggcgtcc acatcttcgg cttcgtgcga aacatccgca
     2641 tcaaacaggg tttttgcgac gattccgagc gtaaggcgca tcatggcttc gtgcatgtca
     2701 acggtttggc cgtcgctcca cgtctccagc atgcgctgcg tgtagtcgac catgatctgc
     2761 gcgtaggccg tgattttttc ctggtgaaac gccggttgaa tgagcttgcg ctggcggcgc
     2821 cagaattcgc cttcgctcaa gagcaaacca ttgcccagca cgcgacgcat ggcgcgataa
     2881 tccttcgatt tcacgaaatt attattttgc gtaacgagga cagactcgat gtagtcaggc
     2941 cggctcaaat agcatgcggg cacgcccatg aaacgaaaaa aaacgatgtc accatattcc
     3001 cgcgtgcatt ttttcaggta ggtgatcgta tcgcctttcg gctcgaacac gctacccagc
     3061 actggaaaac cttttggacc cggaggcagc ttcaactgaa ttagacggac ttccctttca
     3121 ccttcgatga taccggagac ttagcgtcgc cccaaacttc tctcgcgatt tccgccacgc
     3181 ggcgcagctt ggcctcctga tccgcttcgg aaagaagatt gccttccagt gtggaagcga
     3241 atccgcattg cgggctgagg gcgagccgct cgagcggcac gaatttttcg gcggcgtgaa
     3301 tgcggctctt gatttcgtcc ttcgcttcga gcggcgcttt cttcgtgctg atcagtccga
     3361 ggacgacagt gcggtccgat gggacatggg agagcggctc gaagccgccg gagcgctcgt
     3421 catcgaactc cagtaaaaat ctttggaatt ttgtgttccg gaacactttg gcaatgggtg
     3481 cgtaatcgcc cgacgcataa aacttgcttt gattgtttcc gcggcacagg tgcagcccga
     3541 aagttacgtc gggatgcccg ccgatcaccg cgttgtccat ttcaatggaa agatcgagca
     3601 aacggtcggg gtcgtttccg cgctggcgat aaccttcgcg aatgtttgcg tcgatcagtg
     3661 cggcatattg aggcgagtcc acttgaatgt aggtgcagcc aagacgaacg agttcggcga
     3721 cttccccacg cagcagatcg acgaggtcag caagaaagga atcgacagtc ggatacgcgc
     3781 cgctggattt ttttgaatcg tagtaggccg ccgcttgctg agcgctgagt agcgtggtct
     3841 ttgccgtatg atccgtacgc gcgcgcaagt aagtaaattc ttccgcgcac atgtgccact
     3901 tgcggcgcaa cttggaaacc accacgggtc tcggatgatt cgtcacctcg cccttgtcat
     3961 cacggaaagg aatggaccag ccgccgaact tgtcgaaacc ttcgaaggcg tcaatcatgt
     4021 gcccgtaaaa tgcgtaccgg cgcatttcac cgtcggtgag cacgtcgaga ccggcgcgca
     4081 attgcagcgc gaccgattcg tcgactgcgc ggtcttcggt acatttgaat tccgcatcgg
     4141 tcaaacgctg cgccgcaaag tcttcgcggg cttttttcag gtacgcggga cgcagcaggc
     4201 tgccgatcac atcgcttcga tacatgccga actccttttc gtgcttcagc ctaagattag
     4261 tgtgggtggg aggcggacgg gattatatca ctcgagcgca agcgattgaa tatggttgtc
     4321 cgagacgcgt aacatctcct tgtagtaggc cgcttcgtcc aaaatagagt ctcgaaacgc
     4381 ctacatgcgg cccggcatcg aaccaccgta caacaaattt tcaaatcccc ccaatcgaag
     4441 caatgaattg gattgacgcc gaccgctgtt ttactgtacg tccaccatca atgggcccgg
     4501 tggccctcag ccgacgccag gctagcttgt attttttggc tttaaaacga atcgggggtg
     4561 tgcatgtcac taagagaa
//
LOCUS       DASZMB010000206         3439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2683192, whole genome shotgun sequence.
ACCESSION   DASZMB010000206 DASZMB010000000
VERSION     DASZMB010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2683192"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(358..855)
                     /locus_tag="VG272_09365"
     CDS             complement(358..855)
                     /locus_tag="VG272_09365"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWF13928.1"
                     /translation="MAIVDSILMEMDQEAKTTARVLERIPQDKLGWKPHAKSYSLGQL
                     GLHIARGQGFLSDAVSKDTFEIGDMSQPDPTSVKEILDTFAQSTAHAKDTLAKLDDGK
                     LMAMWTATRKGKVLMSVPRIGFIRAIVLNHFYHHRGQLSVYLRLLDVPVPSIYGPSAD
                     ENPFG"
     gene            complement(923..1375)
                     /locus_tag="VG272_09370"
     CDS             complement(923..1375)
                     /locus_tag="VG272_09370"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWF13929.1"
                     /translation="MAKEQLSPETSLTIRRTFLAPRERVFRAWTDPIELGRWFAPSDE
                     YHAIVPEHELRVGGKYCLEMHHKGGNIHRVSGIYKEINPPEKIAFTWLWDHEPKSDES
                     LVTVEFRDLGPSTEILLTHTHLPNAEQTERHGHGWEGCLNMLGNYLNM"
     gene            complement(1339..1713)
                     /locus_tag="VG272_09375"
     CDS             complement(1339..1713)
                     /locus_tag="VG272_09375"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWF13930.1"
                     /translation="MVKYNRELDATFQALADPTRRAIISMLARGQASVTQLARPHRMS
                     LPAIMKHLVVLQRAGIVTQRKVGRTRRCRLAAASLKRAEAWISQYRTFWETQFDSLHR
                     YLAEQPEEDAPWQKSSYPRKPR"
     gene            2059..3150
                     /gene="leuB"
                     /locus_tag="VG272_09380"
     CDS             2059..3150
                     /gene="leuB"
                     /locus_tag="VG272_09380"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HWF13931.1"
                     /translation="MTTQKKKIVLLPGDGIGPEIMRVAVSVLKDCATKFGHDFDFTEF
                     PFGGCAIDATGEPLPAETLAACHAADAILLGAVGGPRWDAVPLAKRPETGLLTLRKSL
                     GLYINLRPIDLHPPLYGLSPLHADRVKDVHIDFVRELAGDIYFGKHDVQPDPSGERAT
                     DEAVYTTSEVERVAKYAFARAEARKGRLTSVDKANVLTTSQLWRRTVTRMSANHPNVK
                     LEHLYVDNAAMQLVLAPAQFDVILTSNMFGDILTDLGAALAGSIGLIPSMSAGPGPSV
                     YEPIHGSAPTLAGKDVANPVGTILSAAMMLREGFNLSREAEYIESAVDRLFTKGFRTP
                     DTVERNTTKVGCVEFGERLQSEMADATYT"
BASE COUNT          768 a          974 c          886 g          811 t
ORIGIN      
        1 gcgggccaac gaagtcgggc gaaattggta ggaagtggca cggttgaaaa agccaagctc
       61 gactttattc agcgcggaat cctcggccac atcggctgcc caccattttc tgaatccttc
      121 ccggacgaaa caggtgggta aactctggcg ggagccgcgt cgatcagaag cgagtgtttt
      181 atgtcggcca tggttcctcc tttgtttgct tgatttggtc taaccgacac ccatgtcgtt
      241 tcactttcga actgctcgcc gtaaaattta actttgtata ttgccttgcg ccaattccag
      301 cctcatgcgt gctggaattg gcgcaacatt tcgtcgatga aatacgcgta gggtccttta
      361 tccaaatgga ttttcgtccg cgctcggccc ataaatggaa ggtacaggca catcgagcag
      421 gcgcagataa accgaaagct gtccacggtg atgataaaaa tgattcaaca cgatcgcgcg
      481 aatgaaaccg attcgcggaa cggacatcaa cactttgccc ttgcgtgttg cggtccacat
      541 ggccatcagt ttgccatcat ccagtttcgc aagcgtatcc ttggcatgcg ccgtactttg
      601 cgcaaacgtg tctagaattt ccttcacgct cgtcggatcc ggttgcgaca tatcgccgat
      661 ttcaaatgta tccttgctaa cggcatcgga cagaaagccc tggccgcgcg cgatgtgcaa
      721 cccaagctgc ccgagcgaat aggacttcgc atgaggcttc catccgagct tgtcttgcgg
      781 gatccgctcc agaactcgcg ctgtagtttt cgcttcctgg tccatctcca ttaaaatcga
      841 atcgacgatt gccatggttg ctcctttgtg tttgctagaa ttccacccat taaaacgctc
      901 taaatctccg acacttttaa acctacatat tcaaataatt tcccagcatg ttcaggcagc
      961 cttcccagcc gtgcccgtgc ctctcggtct gctccgcgtt cggcaagtgc gtatgcgtca
     1021 gcaggatttc cgtggaaggg cccaagtccc gaaactcgac cgtgacgagc gattcgtctg
     1081 acttcggttc atgatcccac agccaggtga acgcgatttt ttccggagga ttaatttcct
     1141 tgtaaattcc gctgacgcgg tgaatattgc cgcccttgtg atgcatttcc aggcaatact
     1201 ttccgcccac tcgcagctcg tgttcgggaa cgattgcgtg gtactcatcg gacggcgcga
     1261 accatcttcc caattcaatc ggatctgtcc acgcgcgaaa tactctttcc cgcggagcga
     1321 gaaaagttct ccgtatcgtc agcgaggttt ccggggataa ctgctctttt gccatggtgc
     1381 gtcctcctcg ggctgttcgg ccagatatcg atggagcgaa tcaaactgcg tttcccagaa
     1441 cgtgcgatat tgcgaaatcc aggcttcggc tcttttcagc gaagcagcag cgaggcggca
     1501 acggcgagtg cgccccactt ttcgttgcgt gacgatcccc gctctttgca gcaccaccag
     1561 gtgcttcatg atcgccggaa ggctcatgcg atgcggccgc gctagttgcg tgacggatgc
     1621 ctggccacgc gcgagcatcg agataatcgc gcgccgcgtg gggtcggcca gcgcctgaaa
     1681 cgtggcatca agttcccgat tatacttaac cattcggttt agtaatatac atttgcacgt
     1741 tccacgtcaa gtcggtattt ttgtccttaa atttttggcg ccgcactcgt ccctgtattt
     1801 ggcaagggtg cgggttttga ttccctacgg aaaattctcg tgagacatcg caagaaccgc
     1861 acctttgaaa aacacaagtg tgcggcaccc aaagtggtcg cttcgtttca agtcccggcg
     1921 acctgacagc atcgtcgatc cggactatac tgtttcgccg cgagcacgca cgaagctgct
     1981 ccgtgcgttg atgaaggcga aaggcttttc gtgacgcaat tgaaattcaa aaatacaagc
     2041 acgcagctcg gaaacaaaat gaccactcaa aaaaagaaaa ttgtgttgct tcctggcgac
     2101 ggaatcggcc ctgaaattat gcgcgtagct gttagtgtgt tgaaagattg cgccacgaaa
     2161 ttcggtcacg atttcgactt caccgaattc ccttttggcg gctgcgcgat tgacgccaca
     2221 ggagagccgc tgcccgcgga gacgttagcc gcatgccacg ctgcggacgc catcctgctt
     2281 ggcgcggtag gcggccccag gtgggatgct gtgccgctcg cgaaacgccc ggaaactggc
     2341 ctacttactc tgcgcaaatc gctcggccta tacatcaacc tgcgacccat cgatttgcat
     2401 ccgccgctgt acggcctttc accgttgcac gcagatcgcg taaaagatgt gcacatcgat
     2461 tttgtgcgcg aactcgccgg cgacatttat tttggcaaac atgatgtgca gcccgatccg
     2521 agtggcgagc gcgccacgga cgaagccgta tacacgacat ccgaggtaga gcgcgtcgcg
     2581 aagtatgcct tcgctcgcgc ggaagcgcgc aagggacgtc tgacgtccgt cgacaaggcg
     2641 aacgtcctga ccacatcgca actctggcgc agaacggtga cgcgcatgtc cgctaatcat
     2701 ccaaacgtga agctggagca tttgtatgtg gacaatgctg caatgcaact tgtgctcgcc
     2761 cctgcgcaat tcgatgtaat cctcacgtcc aacatgttcg gcgatatttt gaccgacctc
     2821 ggcgcagcct tggctggctc catcggccta attccgtcta tgagcgccgg accaggtccc
     2881 tccgtctatg aaccgattca cggctccgcg ccaacgctcg ccggaaaaga cgtggctaat
     2941 cctgtgggca cgattctttc cgcggcgatg atgttgcgcg aaggttttaa tctgagccgc
     3001 gaagcggaat acatcgagtc cgctgtcgac cgcctcttta ccaaaggttt tcgtacgccg
     3061 gacacggtcg aacgcaacac gacaaaagtc ggttgcgtgg aatttggcga acgattgcag
     3121 tccgaaatgg cggacgcgac atacacttag aagcctgcct cacaaatagg cttgcgagct
     3181 acagcttcag tcgcaacgca ggccaacgtg agaattacgg gctttagccc cttaagtatt
     3241 gccaagaccc agctcggggc atttcgtgac atggcttcta gtaaataatt tgtctaacgt
     3301 ccactcaagt gacgaatcgt cacgcctttc gaaaggaaaa cagggagtgc tccggaattg
     3361 gaagcgctcc ctgccgatgt ctacctaaga acgagtctga attatcatct ctactactcg
     3421 ccatcctcga aatcaatgc
//
LOCUS       DASZMB010000207        28826 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1608689, whole genome shotgun sequence.
ACCESSION   DASZMB010000207 DASZMB010000000
VERSION     DASZMB010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 28826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28826
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1608689"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="VG272_09385"
     CDS             complement(<1..910)
                     /locus_tag="VG272_09385"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWF13932.1"
                     /translation="MIGRLRRISLAIMLAVLVTVAPVVPAYAQQQSSAPPEPQPVGRT
                     IPLSTENYTYGKRWFPNIIAPYTQTLVPEPVLTNAPRIEQMVQNGKLTISLQDAVDLA
                     LQNNLAIVIERYVPWIAEANILRTLSGAAPFGGTVNPLGSIPTLSFDPQLTSTISLDQ
                     KTLPVNNGLTGGTGTGTGILSQQATHTTVGDISYTQGFHTGTFFSATFNNTRTSFSNA
                     GNFFFTPVVQSNMVVTGSQELLSGFGRAANEHYIRIAKVNKSIADQTLVQQVITSITA
                     VGNAYWELVFARGNVDVAKQEIALADK"
     gene            1269..1877
                     /gene="pyrE"
                     /locus_tag="VG272_09390"
     CDS             1269..1877
                     /gene="pyrE"
                     /locus_tag="VG272_09390"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HWF13933.1"
                     /translation="MRPDAAIQIFKDVGAIRHGHFELTSGMHSATYVQCAMVLQYPKY
                     AEQLGRALAAEFMDLRVDCVASPALGGLIIGHEVARAIPPGPDGKPIRAIFVERDSLG
                     SLTMRRGFRVEPDEHVLVIEDVWTTGGSTCETIRVLEEAGARVVAAGALIDRSGGIIE
                     LPVRTEALVDLKIESYDASDCPLCRSGGVAVKPGSRFARSGA"
     gene            2081..2890
                     /gene="truA"
                     /locus_tag="VG272_09395"
     CDS             2081..2890
                     /gene="truA"
                     /locus_tag="VG272_09395"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HWF13934.1"
                     /translation="MRNIKFIIAYDGTDFSGWQSQPGQITVQGALSNVLEKLTQHRAA
                     IHAAGRTDAGVHASGQVLNFKTESSMSVEEFQRASNALLPPTIRVNAAEEVHLDFHSR
                     WDALAKTYRYRIFRGRVVPPFIWKYVQQDSSDMNFDAMAEAADLFIGGHDFTSFAAST
                     GSDEDDMERTTVRTIYNSAFLRIPCADSQAVAEEWVYMVRGRSFMRNMVRKIVGTLVE
                     VGRGKLPPSDISKLFDLRDRSKSGVTMPPQGLCLESVEYAQRTESPSVLES"
     gene            3019..3357
                     /locus_tag="VG272_09400"
     CDS             3019..3357
                     /locus_tag="VG272_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13935.1"
                     /translation="MAEETVPQSTSPAAPPPKPPAAPPAATAPPAPAVEAPPVEQKIK
                     VRKPKQRSCDEKDAKGKLCAGHLKRWYDYPKEIVAKIGKDAEIYRCEFCKTLYKPDPK
                     QMPHSFTLRY"
     gene            3462..4733
                     /locus_tag="VG272_09405"
     CDS             3462..4733
                     /locus_tag="VG272_09405"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWF13936.1"
                     /translation="MKRILLLITLGVVVTSFIQNFTRKMAASTAPTDPRVAHVLQVLR
                     DTMGETTEEQIRITEIPAPPFHESVRAAYMKKVLAATGLRVEMDDTGNVIGEWAGSTP
                     EIVMLTAHMDTVFPPNTDVRVKREGGRLLAPGISDNGTGLAALIALSKAFHEAKIKTN
                     KTILFVADVGEEGEGNLRGMRALAEAYKKRLKYVLALDGSAVEYVTTAALASRRVEIT
                     ITGPGGHSWSDFGAPNPIHAMGRGIARFVTARVPELPRTSFNIGEISGGTSVNSIPSS
                     AQMKVDLRSESEIELAAVETQLRDAVKAGIDEEMSAARERGMAGGANLLNLKINVLGV
                     RPAGELSENSPLLAALLAADNQLGNRSRRERSSTDANIPLSMGIPAISIGAGGRSGGA
                     HTLDEWYDPSGRELGLQRVALTVLTLAGIEQ"
     gene            complement(4793..6772)
                     /locus_tag="VG272_09410"
     CDS             complement(4793..6772)
                     /locus_tag="VG272_09410"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF13937.1"
                     /translation="MPLRMIAPIRHHIWIKTGPAALFIFAALLCPISHAADKEKNKDK
                     PQKEKKEKIEPWVEIRTTHFIVASDGGEKTALRFANQFETLFRVFQATMPNARVTNGI
                     PVRLLVARDGQSFARMAPEFPYDKKRDQPPALIATGSEKTYIGIRANVGGRFPFADIF
                     KTYAREIVKRSYRNLPPWLEEGFSTIYAGVTFTDRGVRLERPDPEDLSVLFESPLLPL
                     DLVFHVDRNSGYYSPGDTQSVYFAESRVLVHFLIVDPRISGSKSMERYIAAVQSGTDS
                     LQAARDAFGDLNQLQTRFSAFVNEMKGAPTDIPVSGGNESGSAPRTLSAPEIEARRSD
                     FLSLRGRMDDAQGDLEEAINTEPSLAEAEQSLGFLLLKKRNLDDAEKHFQHAVQLDPK
                     DALNFYGQGLVAMAKSGISGQPAAAISALEKAATLNPDFAEIWYNLALIYSQKDETLQ
                     KALAASQHATSLAPGNSNYQLQLASIQNEMGHPDEARKTAAMVQGSTDDKNTAKKAAE
                     LVARTSTPQPPAAAKPTSESVKITSAPPPPAPAVKAASPKSEPAPTPVPNAAAPPAAP
                     LFSEAKVYSMVGTITEVTCTSASEIQLTLKSLTILMKLHSVDLAKLPVRSAGSENSSK
                     ISSCSSLRGRNVRISYNLVLDKPWDGEMQELEFRN"
     gene            6988..7878
                     /locus_tag="VG272_09415"
     CDS             6988..7878
                     /locus_tag="VG272_09415"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWF13938.1"
                     /translation="MSNRFKAHCALMACSFFWGVTFVVVKNALADISVFAYLSARFML
                     GALPMIWIYRDDLRNLTRDEAWAGIQVGLFMATGYAFQTAGIARTTPSKAAFITGLSV
                     VLVPIFLAVFWRRKIGAWAWGGAAGSFAGLYFLTVPAEGFSQLNHGDMLVMGCAVLYA
                     MQIIYIARFTGKYSLGALSCLQVILTGVLSAIAVPVFNVTGLEHFFVRFTAQMEFGVI
                     VTAIFTTALAYPLLVWGQTHTTATNTALILTSEPVFAALTSFVMLHERLGGRALAGAI
                     LILGGIGIAEWKGTVPSAGS"
     gene            complement(7982..8620)
                     /gene="leuD"
                     /locus_tag="VG272_09420"
     CDS             complement(7982..8620)
                     /gene="leuD"
                     /locus_tag="VG272_09420"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HWF13939.1"
                     /translation="MQTFSRHTGLVLPFDRVNVDTDQMVPKQFLKLQTKEGYGKVLFY
                     DWRYMADEKVNPDFIMNWPRYKGVSILLARAGFGSGSSREHAPWAIADYGFRVLIAPS
                     FADIFFSNCFKNGILPIIFPEPQIDELFHRAEKQEGYSLTVDLPAQTVSDASGLQYRF
                     EIAEFRKHLLLEGLDDIGLTMKHEAKIADFEKSHHPSAILYAPVDAKYASRQ"
     gene            complement(8717..10138)
                     /gene="leuC"
                     /locus_tag="VG272_09425"
     CDS             complement(8717..10138)
                     /gene="leuC"
                     /locus_tag="VG272_09425"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009116.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HWF13940.1"
                     /translation="MNPRTMYRKIWESHLVREAPGEPSLIYIDRHLIHEVTSPQAFAG
                     LKTAGRKVRRPDLTFAVMDHSVPTKDRNLPIMDKAAGGQFDALERSCRENGITLFDMF
                     SHNQGIVHVIGPELGLTQPGQTLVCGDSHTSTHGAFGTLAFGIGTSEVEHVLATQTMQ
                     QYESKTMHIVVNGKRTHGVSAKDIILAIIGKIGIGGGAGHVFEYAGEAIRALSMDGRM
                     TVCNMSIEGGARAGMIAPDDTTFAYMEGRPYVPRGKEFQDAVQNWKALASDPGAKFDA
                     TVELDASSLAPQITWGTNPGMVAGVTDRVPDPGAFRDPDDQKAVERALTYMGLKPGTA
                     LVDIPVDRVFIGSCTNSRINDLRAAARVVAGKHVAKNLKGALVVPGSMTIKEKAEAEG
                     LDKIFRDAGFEWRDAGCSMCIAMNPDVLLPGERAASTSNRNFEGRQGKDSRTHLVSPI
                     MAAAAAIAGHFVDVRDWNVEATE"
     gene            10780..12390
                     /locus_tag="VG272_09430"
     CDS             10780..12390
                     /locus_tag="VG272_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWF13941.1"
                     /translation="MGMQLREFANWARNRKILATLLVTVTLAVGMLIGTVISGHVGAA
                     HPAAAGAIGGTPLAIPDPVQLSSAFASIVKQVQPAVVNISTTQVIEQHGNGKGRGPSP
                     RNRGGEMGPPGDQDQDQQPFQDFFNRFFDFPDQGPTAERSLGSGVIVDKKGFILTNNH
                     VVDQATKVQVTLNGEQTQYTAKVVGIDEETDLAILKIDVGHDLPFAKLGNSDSVQVGD
                     WALAIGNPFGFLQGSVTAGIISAKDRGNVGQQFQRFIQTDAAINPGNSGGPLVDMAGQ
                     VIGINTAIITGGHGNEGVGFALPSNTATGVYNQIVSNGKVTRGSIGVSFTETQGSNPI
                     VLRELGATYGIVLQRVEPGSPAEKAGLQSGDVVTSINGKPVHNGNELVNPIAQTPIGN
                     SVRVTYLRDKKEHEASITVADRTKLFPDRAAVAGGNESGPTPAEFGLRLEDLGTDRAR
                     RAGYENMKGALVTAVDPASFAEDVGFTRGDVITELNHTSVNSVAEYRRAVSGLKPGQD
                     VLFKVMRRSGSDQFDTVYLAGAVPAPEQ"
     gene            12429..12920
                     /locus_tag="VG272_09435"
     CDS             12429..12920
                     /locus_tag="VG272_09435"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HWF13942.1"
                     /translation="MIKRIRLNLFGVALLTCATPILFAAARPAAAQSSFSSAKKKKKT
                     VTTATKTKGKSKGRVAARPKGQAAPTADRIKEIQSALQKDGSYDGEPTGKWDAATTDA
                     MKKYQDKNGVNPTGKIDAVSLNKLGLGSETAGKGAPIPAANSSTAPAPASKPSTSVSP
                     TSE"
     gene            complement(12948..13847)
                     /locus_tag="VG272_09440"
     CDS             complement(12948..13847)
                     /locus_tag="VG272_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13943.1"
                     /translation="MNLKITTTALIFPVIFLAASVSAIAQGSSIPTGRTTDRLDWNES
                     FEGSTGSSGQEMELSSSATFHFGRYSVGAGVPVYFNRAILPNGVTTSEGVGDFSVTLG
                     ATWNYSILNYATALTGSAPTGNSSKGFSTGHATFDWNNRIDHDFSLLSPFLDAGVANT
                     VTDTQYFHRPFTSYGYLAHGEAGADIDLTHSFTFTVSGYKIAPWGTQTIISRDVVKGA
                     IGSGGSNGRVFEVNHLTTGPASLNYDDGFTTGLSFSPKPYLDLDLGFTRSMSFAYNTF
                     SWGINVNMSRLISRENPIHSDRK"
     gene            complement(14014..14568)
                     /locus_tag="VG272_09445"
     CDS             complement(14014..14568)
                     /locus_tag="VG272_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWF13944.1"
                     /translation="MESFGEALSGMPGRKLKRQCTPYRNLHMKIALIVLCIALGLLIA
                     GAALALWQFNLSAMTAPDNAEISLAGKLRHFYIRRASRAPISSPPANQQASIEEGDKL
                     FGTECAACHGLSGNKPTDAGRWMYPRAADLTLADVKQYSDRELFWIIRNGIRFSGMPA
                     FGNVEPDEHIWNLVQYVRTLPAAN"
     gene            complement(14707..15084)
                     /locus_tag="VG272_09450"
     CDS             complement(14707..15084)
                     /locus_tag="VG272_09450"
                     /inference="COORDINATES: protein motif:HMM:NF039244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5818 domain-containing protein"
                     /protein_id="HWF13945.1"
                     /translation="MRKTIIGVAGSLLLSVVIFAAPKDSTYTGEIMDNQCAKNASHAA
                     MLKKEGMGDKDPNDAMTKKMCTQNCIKMGGKYVLYNSKTKTVYELDDQSKPADFAGAN
                     VKVSGTLDKATKTIHVTNIEAAS"
     gene            complement(15131..15388)
                     /locus_tag="VG272_09455"
     CDS             complement(15131..15388)
                     /locus_tag="VG272_09455"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HWF13946.1"
                     /translation="MRTNFMAMMDCKEVLANLSSYVDGEASSELRASLEDHIAKCRRC
                     RVVFDTTQKSLRIVLDVEPFEVPLAVSARLYARLEKELSAS"
     gene            complement(15385..16071)
                     /locus_tag="VG272_09460"
     CDS             complement(15385..16071)
                     /locus_tag="VG272_09460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF13947.1"
                     /translation="MNEFPKTSEVPSDEDLALIRLALAGDRQAFETLVRRHEKRVFRV
                     TLAVLGNVEDAEDAMQETFIKTYRNLGHFRGDSKFTTWLTRIAVNESIQKRQSRRSSV
                     PLEESTEVECKSIPGRFEPWLTNPEKLYGKQEVRRLVEEAIRALPLIYRETLVLRDIE
                     EMSAEEAAEALGIKVPALKSRLLRARLLLRESLAATFEQRPTLGRKVLHAATDFGTGM
                     AMRMMRMAGK"
     gene            16289..17314
                     /locus_tag="VG272_09465"
     CDS             16289..17314
                     /locus_tag="VG272_09465"
                     /inference="COORDINATES: protein motif:HMM:NF022818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3179 domain-containing protein"
                     /protein_id="HWF13948.1"
                     /translation="MRRRAWLQAMGIILISASANVASAAQKGGGPPMPFVPLREPHFV
                     TASRADFLKADDRVVGVSENGVTKAYEPSVLAFHHVVQDNLKDMPIIATWCALCNTPL
                     VYSSVVDGKKLTFERAGNRGNNFFMLDTETGSHWQQIGGDCFEGPLKGKRLTMVPFLY
                     TTWGEWREQHPDTLVLVPEAAYKAGYEFMEKSRVSVVPYGSNKKPDRELVRDFDTRLP
                     NYEQVIGIEIGDLHKAYPLSALKKETVVNDKVGSIPILLLYAAGSDTTTGFSRILGGR
                     TLTFRAAALGSFMDNETGSNWSVYGNCLDGKLKGQKLERVIPQPGSWFAWAEFHPDTE
                     IYQSGSH"
     gene            17737..19239
                     /locus_tag="VG272_09470"
     CDS             17737..19239
                     /locus_tag="VG272_09470"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWF13949.1"
                     /translation="MTTSAISGSHKNFINGEWVEARRGGVFENRNPARTSELVGIFPS
                     STSEDVDDAVDAAKNAFKHWRLFPAPRRAEILYRTAETLVKRKEDLAREMTREMGKPL
                     SETRGDVQEAIDMTYYMAGEGRRLFGQTVPSELPNKFAMSVRDPLGVVGMITPWNFPM
                     AIPSWKIMPALICGNTVVIKPAEDTPLSTYNLVQILIESGLPPGVLNLVAGMGPDAGA
                     PLITNPGVSAVSFTGSTETGRIVSQACAPTFKPCHLEMGGKNIMIVMDDANLELAVEG
                     AVWGGFGTSGQRCTAASRVAVHSKIYKEFLKLFTERTRALKLGDGLDPQTDVGPVVNE
                     TQLFTVMKYVEIGKSEGAHLAVGGHRAESGALAKGWFHEPTIFSDVDAKMKIAQEEIF
                     GPVVAMMPFSSLEGAIEIANNVKYGLSASIYTRNVNQAYSAMRDLQTGIVYVNAPTIG
                     AEIQLPFGGTKGTGNGHREAGTAAIDFYSEWKTLYVDYSDKLQRAQIDTP"
     gene            19520..20563
                     /gene="hrcA"
                     /locus_tag="VG272_09475"
     CDS             19520..20563
                     /gene="hrcA"
                     /locus_tag="VG272_09475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HWF13950.1"
                     /translation="MSETTPNSERNRQILTAVVRAYIETGEPVSSRSISRRHVEALSS
                     ATIRNVMADLEEEGYLFQPHTSAGRVPTANAYRFFAQQIVTGATLSAQDEDLIRSEIS
                     GASSGEEIAERAGHVLAEISKGLGIVVSPPISKTVLEHVRFVLLPDSRIVVVLVSTGG
                     NTRDKVIRPEKVFSQQDLDHTAEYLNHHYTGFLLEAIRADLQKRLAQEEERYAMLARN
                     ALELCDPGVMGQGSAQRIYIEGAAQMAAAPEFSGPSQIRDVLEAIEEKHRIIALLANC
                     IDTPDPVHVQIGVKGMDRAGEHLALISAPYSFQDQSQGTVGVLGPMRMQYERAITAVA
                     FVARAFSETLDRS"
     gene            20569..21189
                     /gene="grpE"
                     /locus_tag="VG272_09480"
     CDS             20569..21189
                     /gene="grpE"
                     /locus_tag="VG272_09480"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HWF13951.1"
                     /translation="MAEAKREEKKVMPIDGNPSEQDNESQNIAEDPISVDPQAGIDSA
                     LAAEVQKLKAEKEDLMQTMMRRQADFENFRKRTERDRSEEAKRGVERLVLDLIPVIDA
                     FDRALLAHDDPRYEEYRKGITLIRKQLWDAIAKHGVLPIEAAGKIFDPHHHQAIERVE
                     SEEYPDGTIVAVFQDGYMFNGRVLRPSIVRVAVHPDEGENSTSNEN"
     gene            21211..22350
                     /gene="dnaJ"
                     /locus_tag="VG272_09485"
     CDS             21211..22350
                     /gene="dnaJ"
                     /locus_tag="VG272_09485"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HWF13952.1"
                     /translation="MSEKRDCYDILGIPRTAGADEVKSAYRKAALKWHPDRNPHTKSE
                     AEEKFREATEAYSILSDVQKRATYDRFGYQGLSRGGFDGGVNQTIFDEFQDIFGDFFG
                     FEDVFGGRGGSGRGRSRSQRGADLRYDMKLSFEEAAAGVNTKIRIPRQEFCEVCNGTG
                     AKAGTGVVSCATCGGRGQVHYQQGFFTISRTCPECRGAGQIVRESCPDCKGKGRIERS
                     RTIDLRIPPGVDSNTRLRIPGEGEPGGNSGPAGDLYVVLEVKPHPYFERRDADLYCTV
                     PVSIVQAALGTEIVVPGLNGEEHVKIPEGTQTGEIIRLKGKGMPDPHGGGKGDLYVNI
                     HVEIPTKLTREQRRLFEQLGTTLPVENRPAHRDSSLFEKVKDIFG"
     gene            22611..23360
                     /locus_tag="VG272_09490"
     CDS             22611..23360
                     /locus_tag="VG272_09490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HWF13953.1"
                     /translation="MRRRFFVDEFENGSAILRGEGAHHLGRVLRAEPGQLYELSDGQC
                     LWLARTQSVGRDEVQFTLVEQLPATTAPVRIDLLLAIVKFDRFEWALEKATELGADEI
                     TPLAADRSEKGLIAASGKRAERWKRILGESAQQARRLHVPGLAAAAKPREAFRAPAAQ
                     LKLMFSERAGAQPLRDVLDPVAVAVRENETACVAIAIGPEGGWTDAEFAASIECGCVE
                     VALGTNILRTETAVCAALAAVQYAFGAFRQK"
     gene            23925..24512
                     /gene="lepB"
                     /locus_tag="VG272_09495"
     CDS             23925..24512
                     /gene="lepB"
                     /locus_tag="VG272_09495"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWF13954.1"
                     /translation="MEPNAEISTSVAPGAAQPLRRELRSWGRDLAIALSLAVVIIIFF
                     YQPVKVEGTSMTPLISDQERIFINKFVYRFEPIERGDVVVFWYPLDRSKSFIKRVVGL
                     PDDTVEIRGGQVFLNGRLLPEPYIPPESDDYGTYSPMQVPKGEYFVMGDHRTSSNDSR
                     IFGPVPRRFIYGKAVFAYWPVDHFGSIHVFAESAK"
     gene            24687..25859
                     /gene="carA"
                     /locus_tag="VG272_09500"
     CDS             24687..25859
                     /gene="carA"
                     /locus_tag="VG272_09500"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HWF13955.1"
                     /translation="MLALEDGRIFYGRAAGAVTRRGGEVVFNTSLTGYQEVFTDPSYA
                     GQIVCLTYPHIGNVGANSEDEESSRPHIESLIVREFSAVSSNWRSAETAENYLVRNNI
                     PVLWDIDTRALVLHIRKLGALRGVVSTDGTPAEKLLAEARALPSMAGLELASRVTCEK
                     QYGWGKGSIELTVPPWQEAVGESEGEKKSPKFRVVAYDYGIKQNILRLLTDHQCEVVV
                     VPAKTSAEDALALKPDGVFLSNGPGDPEPATYAIQTIRDLLGRVPVFGICLGHQLCGL
                     ALGGKTFKLRFGHHGSNHPVKNLLTGQVEITAQNHGFCVDPDSLPASDVEVTHLNLND
                     HTNEGFRHRTLPLFSVQYHPEASPGPHDSHYLFRQFTDTMKSWQSSGATRRGQAKA"
     gene            25866..>28826
                     /gene="carB"
                     /locus_tag="VG272_09505"
     CDS             25866..>28826
                     /gene="carB"
                     /locus_tag="VG272_09505"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HWF13956.1"
                     /translation="MPKRTDIKKIMIIGSGPIVIGQACEFDYSGTQACKALRSEGYEV
                     VLVNSNPATIMTDPEIADRTYIEPLTKQYIEKIISIERPQALLPTVGGQTALNLAVEL
                     AEGGILAKYNVELIGASLHAIKVAEDRLLFKDAIRKVGLDVPASALINNVQDGLKLAD
                     QLGYPVVIRPSFTLGGTGGSIAYNREEFSEAMAHALDASPVHEALVEESVLGWKEYEL
                     EVMRDYRDNFVVICSIENFDPMGVHTGDSITVAPAQTLTDKEYQRMRDAAAVIIREVG
                     VETGGSNIQFALDPATGRMIVIEMNPRVSRSSALASKATGFPIAKIAALLAVGMSLDE
                     IPNDITKKTPSCFEPTIDYVVAKIPKWQFEKFAGADSTLGTQMKSVGEVMAIGRTFKE
                     ALQKGIRSLEPSSPWRAPADTPVSLLREKIATPRPDRIHWLFVALERGISAKEAIDLT
                     KIDPWFIEQMLEIIAVNKRLASVTMETVPEDLLREGKRIGTSDEQLAQMWKTTPAAVR
                     HRRAKAHIAPVFKRVDTCAAEFESFTPYMYSTYEEEDESQPTDRPKIAILGSGPNRIG
                     QGIEFDYCCCHASFALREDGYETIMINCNPETVSTDYDTSDRLYFEPLTLEDVLSVVD
                     REKPQGVIVQFGGQTPLNLALELKRNGVPIIGTAPESIDLAEDRRRFGKLLDELKIPQ
                     PKNGTALVPEEAARGAGEIGFPVLVRPSYVLGGRAMVIAYDLNTVQEYVAQAALMGPA
                     KPVLIDQFLEEATEVDVDALADGEDVIIGGVMEHIEEAGVHSGDSSCVLPPVSLSPTV
                     LAKIRDYTNRLAKALQVVGLMNVQYAIQRDTVYVLEVNPRASRTVPFVSKATGVPLAK
                     VAARLMAGRKLREMNLPVVITDGVAEIAVKDYYSVKSPVFPFNKFRGVDTILGPEMRS
                     TGEVMGVAKSYGQAFAMAQLAAGQRLPRKGSIFLSVSDHDKRHCGPLAKELHALGFRL
                     IATRGTAAALEAAGV"
BASE COUNT         6594 a         7564 c         8115 g         6553 t
ORIGIN      
        1 ttttatccgc gagggcaatt tcctgcttcg ccacatccac gttgccgcgc gcgaatacca
       61 attcccagta cgcatttccg acggctgtga tggaggtgat cacctgctgc acgagcgtct
      121 gatcggcaat gctcttattg accttggcaa tgcggatgta atgctcgttg gcggcacgtc
      181 caaaaccact caacagctct tggctgccag taactaccat gttggattgc acgaccggag
      241 taaagaagaa gtttcctgcg ttggaaaagg acgtccgcgt gttattgaaa gtagccgaaa
      301 agaaagtgcc agtatgaaat ccttgggtat aactgatgtc tcctacagtt gtgtgcgtgg
      361 cctgctgaga gagaattcca gtgcccgtgc ctgtaccgcc agtgagtcca ttattcacgg
      421 gtaatgtttt ttgatccagg ctgatcgtgg aggtgagttg cggatcgaag ctcaacgtgg
      481 ggatggaccc aagagggttt acagtacccc caaacggcgc ggccccgctc aatgtccgca
      541 agatatttgc ttcggcgatc cacggcacat accgttcgat cacaatggcc aaattgtttt
      601 gcagtgcgag gtctacggca tcctgcaggc taatggtcaa cttgccgttt tgcaccattt
      661 gctcgatgcg cggtgcgttc gttagcacag gctcaggtac cagcgtctgg gtgtagggcg
      721 caatgatgtt ggggaaccag cgcttgccgt atgtgtagtt ctccgttgac aggggtatcg
      781 tgcggcccac gggctgaggc tccggcggtg ccgacgattg ctgctgggcg tacgcgggca
      841 caacgggtgc cacggtcacg agaaccgcca acataatggc gagtgagata cggcggagcc
      901 ttccgatcat ttttaagttt tcctctccat acagcaatcc agtatttaat tgcgcggcga
      961 aacggtaacg taagatggct aagtgattta aacgtgcgtc aggactgaat tgtttcaact
     1021 attttgcaca agtttccccg gaatcgcggg ttaacaatct atccaccccg aacgcgaaca
     1081 ttgtctggga aatccaccgc gaaataatat ggttaagtaa atttattcag ccacttttag
     1141 cgcccgagta ttggacgcga gaaactgcgg aaggtcaaga ccttaagtca attatcgggc
     1201 cggttttttg ccaagacaag tctgctgcct tgacaaccgt atagggggac cgataggctt
     1261 ggcgcgggat gagaccagac gccgctattc aaattttcaa agacgtgggg gcaatccgcc
     1321 acgggcattt tgaattgacc tctggcatgc acagcgccac gtacgtgcaa tgcgcgatgg
     1381 tcttgcagta tccgaaatac gcggagcaac tgggccgtgc gctggcggct gaattcatgg
     1441 acttgcgcgt ggattgcgtt gcatcgcccg cactcggcgg gctaatcatt ggccatgaag
     1501 tggctcgtgc gattccacct gggcctgacg gcaaaccgat acgcgccatt ttcgtcgagc
     1561 gcgattctct gggatcgctg actatgcggc ggggattccg cgtcgagccg gacgagcatg
     1621 ttttagtgat cgaagacgtt tggaccaccg gtggttcaac ttgcgagacg attcgcgtgt
     1681 tggaagaagc gggggcgcgc gtggttgccg cgggcgcttt gatcgaccgc agcggcggaa
     1741 taattgagtt gccggttcgc accgaagcgc tcgtcgattt aaaaatagaa agctacgatg
     1801 cctcggattg ccctctctgt cgaagcggcg gcgtagccgt gaagcccgga agccgctttg
     1861 cgcgttccgg cgcttgaaac cagggatgcg cacggataaa cgcggataaa agccaaggta
     1921 aaatattttg aatcaaaaag gcccgtctta cttcggttgt aaacaattgc gagacaatcc
     1981 atatttctca caaagttcat tttaatcctt gcagtatgtt ttatggattt ttatccgtgt
     2041 tcatctgtgt gcatccgtgg ttccaacgca cttgaattac atgcgaaaca taaaatttat
     2101 cattgcctat gacggcaccg atttcagcgg ctggcaatcg cagcccgggc aaatcacggt
     2161 gcagggtgcc ctttccaatg ttcttgaaaa attgacgcag caccgcgctg cgattcacgc
     2221 cgcggggcgc acggatgccg gcgtacacgc gtccgggcaa gttcttaatt tcaaaacgga
     2281 atcgtcgatg agcgtcgagg aatttcagcg cgcgtctaac gctctgctgc cgccgaccat
     2341 tcgcgtgaat gccgcggaag aagttcatct tgattttcat tcacgatggg acgctctagc
     2401 aaaaacatat cgctaccgaa ttttccgcgg gcgtgtggtt ccgccgttca tttggaaata
     2461 cgtgcaacag gattcgtccg atatgaattt cgatgccatg gccgaagccg cggatctttt
     2521 tataggcggc catgatttta cttcgttcgc ggcttcgacg ggatcggacg aggatgacat
     2581 ggagcggacc acggtccgga cgatttacaa ttccgcattt ctgcgcatcc cttgcgcgga
     2641 ttctcaggcc gtggccgaag aatgggtcta tatggttcgc gggcgttcgt tcatgcgcaa
     2701 tatggtgaga aagatagttg gcacgctcgt ggaagtcggc cgcggaaagc ttcctccttc
     2761 tgatatttcc aagttgttcg acctgcgcga tcgatcaaaa tccggcgtca ccatgccgcc
     2821 gcaggggctt tgcctggaaa gtgtcgagta tgcgcagcga acggaatctc catccgtgct
     2881 tgagagttga gcgaaagggg cgaagccagc gctcccaaac tcgcgctttg tttcacgaga
     2941 agggttcgag taagatacat cccagaaccg ctgagaccga tcagaacgcg aagtctgatt
     3001 tgagaaggga accgtcgaat ggccgaggag acagtaccgc aaagcacatc accggcagca
     3061 ccgcccccaa agcctcccgc agcgccgccc gcagcaactg cgcctccggc acccgctgtc
     3121 gaggccccgc cggttgaaca aaagattaaa gtgcgcaaac cgaaacaacg gtcctgcgac
     3181 gaaaaagatg ccaaaggaaa actctgcgcc gggcacctca agcgctggta cgactatcca
     3241 aaagagattg tggcgaagat cgggaaagat gcggaaattt accgctgcga gttctgcaag
     3301 acgctgtaca aaccggaccc gaaacaaatg ccgcacagtt ttacgttgcg ctattagtcg
     3361 cgcaatatag aggcacgatg tctgttagcc gcagtggcac caatatattt gtgcacgatc
     3421 cttccgagac gagggccgat gcttctgcgg ccgagtgagc cgtgaaacga atcctcttat
     3481 taattacgct gggagtagtg gtgacgtcct ttatccaaaa ctttactcgc aagatggctg
     3541 cctctaccgc gccaaccgat ccgcgcgttg ctcacgtatt gcaagttctt cgcgacacca
     3601 tgggcgaaac caccgaggaa caaatccgaa tcacggaaat ccccgcgccg ccatttcatg
     3661 aaagcgtgcg cgcggcatac atgaaaaagg ttttagccgc cactgggctt cgcgttgaaa
     3721 tggacgatac cggaaacgtt attggggaat gggccggatc gactccggaa atcgttatgc
     3781 tgacggcgca tatggacacg gtttttccac cgaataccga tgtgcgcgta aaacgtgaag
     3841 gtggccggct gcttgcgccg ggcatctcgg ataatggtac cgggctggcc gcgctcatcg
     3901 ctctgtcaaa agcctttcac gaagcaaaaa taaagaccaa caaaacaatt ttatttgtgg
     3961 cggacgtagg cgaagagggc gaaggaaatc ttcgcggaat gcgcgcgctg gcagaggctt
     4021 ataagaagcg gctgaaatat gtgctggccc tcgacggctc agcggtggaa tatgtgacca
     4081 ccgcggcgct tgcctcacgt cgcgtggaga tcacgattac cgggccgggc ggccatagct
     4141 ggtcggattt cggcgcgccg aatccgattc atgcaatggg ccggggcatt gcgcgatttg
     4201 tcacggcgcg cgtaccggaa ttgccgcgca ctagtttcaa tatcggcgaa atttcgggcg
     4261 gcacttcggt gaattccatt ccatccagcg cgcaaatgaa agtggatttg cgctccgagt
     4321 ctgaaatcga acttgccgcg gtcgaaacac aattgcgcga cgcggtaaag gccggcatcg
     4381 atgaagaaat gtccgcggcg cgcgaacgtg gtatggcggg cggcgcaaac ctgctgaatc
     4441 taaaaattaa tgttttgggt gtgaggccgg cgggcgaatt atcggaaaac tcgccgctct
     4501 tggccgcctt gctggctgcc gataatcagc tgggcaaccg ctcgcgccgg gagcgttctt
     4561 cgactgacgc aaatattccg ctctccatgg gcatccccgc gatttcgatt ggcgcaggcg
     4621 gaagaagcgg cggcgctcac acgctggacg aatggtacga tccttcaggc agggaactcg
     4681 gcctgcagcg cgtggcattg acagttctta ccttggcggg catcgagcaa tagtttcttc
     4741 cgcgcaatgc cggcttggct gcgtatttcc cacaaaaaat cagaattccg tgctaatttc
     4801 tgaattccaa ttcctgcatt tctccgtccc acggtttgtc caaaaccaaa ttgtaggaaa
     4861 tgcgcacatt gcgtccacgc aggctcgagc acgatgatat tttcgatgaa ttttctgagc
     4921 ctgcggatct gaccggcagc tttgccaagt ctacggagtg aagcttcatg agaatcgtga
     4981 gggatttcag cgtcagctgg atttcagatg cgctggtgca cgtcacttcc gtaatcgttc
     5041 ccaccatgga ataaactttc gcttcggaaa atagcggagc ggctggtggt gctgccgcat
     5101 taggaaccgg agtgggagcg ggctcggatt taggggacgc ggctttgacc gctggtgccg
     5161 gaggtggagg ggccgaagta atttttacgg attcagatgt cggttttgcc gcggcgggcg
     5221 gctgaggcgt agaggtcctg gccacaagtt ccgcggcctt ctttgccgta tttttatcgt
     5281 ctgtggatcc ctgcaccatt gccgcggttt tgcgtgcttc gtcaggatgt cccatttcat
     5341 tttgaatcga ggccaactgc aactggtaat tggaatttcc gggcgcgagc gaggttgcgt
     5401 gttgtgaggc tgccagggcc ttttgtagcg tttcatcctt ttgcgaataa atcaaagcca
     5461 ggttgtacca gatttccgca aaatccggat tcagcgtagc ggctttctcc agggcactaa
     5521 tcgccgcggc aggctgacct gaaattccgg acttggccat tgccaccaga ccttgcccgt
     5581 aaaaattgag tgcatctttc ggatcgagct gcacggcgtg ctggaaatgc ttttccgcat
     5641 catccagatt gcgctttttt agcagaagaa atcccaggct ttgctctgcc tccgcaagcg
     5701 aaggctccgt gtttatagct tcttcgagat cgccctgcgc gtcatccatt cttcctcgca
     5761 gggaaagaaa atcagatctg cgcgcctcaa tctccggggc ggaaagtgtg cgtggggcgc
     5821 tgcctgattc attccctcca ctaaccggaa tatccgtggg agctcctttc atctcgttga
     5881 cgaacgctga gaatcttgtt tgcagttgat ttagatcccc aaatgcatcg cgtgccgctt
     5941 gcaaggaatc ggtcccgctt tgcacggcgg caatgtaacg ttccatggat ttcgatccgg
     6001 aaattcgcgg gtccacgatg aggaaatgca ccaggacgcg cgactccgca aaataaacgg
     6061 attgcgtatc tcccggactg taatagccgg aattgcggtc cacgtggaaa accagatcga
     6121 gcggaagaag aggactttca aagagcacgc ttaaatcttc ggggtcgggc ctttccagac
     6181 gaacgccgcg gtcggtgaac gtgacgcccg cgtatatggt tgaaaaaccc tcttcgagcc
     6241 agggaggcag gttgcggtag ctgcgcttca caatttcacg cgcatacgtc ttaaaaatat
     6301 cggcaaacgg aaatcggccg ccgacattgg cgcgaatgcc aatgtaagtt ttctctgaac
     6361 ccgtggcgat cagcgcgggc ggctggtcac gcttcttgtc atacgggaat tcgggagcca
     6421 tgcgcgcaaa cgattgcccg tctctcgcca cgagcaagcg aaccggaatg ccattggtga
     6481 cgcgagcatt gggcatggtg gcttgaaaca cacgaaacaa tgtttcaaac tgattcgcaa
     6541 atcgcagcgc tgttttttcc cctccatcgc tggccacgat gaaatgagtt gtgcggatct
     6601 cgacccacgg ctcgattttt tccttctttt ctttttgcgg cttgtcttta ttcttttctt
     6661 tgtcagcagc gtgtgaaatg gggcaaagca gggccgcgaa aataaatagt gccgccggac
     6721 cagtctttat ccagatgtga tggcgaattg gggcaatcat tctgagtggc aacgagccgt
     6781 ttctccgtaa gtttaccaga catgccttcc ggctggcgcc agaatcgtct ttggtgcgat
     6841 tccatcgggg gcaatctggt tgcctttaga gcgtcgaatc aatagactcg aacgtttaat
     6901 gggacttgcg cccaaattgc cgggaagatc gatcaccaac tcaattgacg tacgaatttc
     6961 atctctggat cgaaataggt atgcacggtg agtaaccgtt ttaaagccca ttgtgccctg
     7021 atggcgtgct cctttttctg gggagtcact ttcgttgtgg tcaagaacgc tttggccgac
     7081 atctccgtat tcgcctatct ctccgctcgc ttcatgctcg gcgcgttgcc catgatctgg
     7141 atttacaggg acgatttgcg gaatctcacg cgcgacgaag cctgggctgg aatccaggtg
     7201 ggacttttca tggcaacagg ctacgcattc cagacggcgg ggatcgcgcg cacaacgccg
     7261 tcgaaggcag cgttcattac tggactcagc gtcgtccttg tccccatttt tctggccgtg
     7321 ttctggcgaa gaaaaattgg cgcatgggcc tggggaggcg cggccggatc gtttgccgga
     7381 ctttatttcc tgaccgttcc ggcggagggt ttctctcagc tcaaccacgg agacatgctg
     7441 gtgatgggtt gcgcggtgct ttacgccatg cagattattt acattgcgcg cttcaccggg
     7501 aaatattcgc taggagcgct cagttgtctg caagtaatcc tcacgggagt gctttccgcc
     7561 attgcggtgc ctgtattcaa tgtcactggt ttggaacatt tcttcgtccg cttcacggct
     7621 caaatggagt tcggagtcat cgtcaccgcg atttttacca cggcgctggc ctacccgctg
     7681 ctcgtctggg gtcaaacgca cacgacggcg acgaacactg ctttgatctt aacttccgag
     7741 cccgtcttcg ctgctctcac gtcgtttgta atgttgcacg aacggctcgg tggccgcgcc
     7801 ctcgcgggag ctattcttat tttgggcgga atcggtatcg ccgaatggaa gggaactgtg
     7861 ccttcggcgg gatcttaaat tcttgcgaac gcggacttcc gcgggaaaat tgtcttagcg
     7921 tgatattttt tccttttttt cttgtgcgca aatcttcggc acgcaaagtg atggaaagca
     7981 attactggcg gctagcgtac ttggcatcca cgggcgcata caggatggcg ctgggatggt
     8041 gcgatttttc aaaatcggca atttttgcct cgtgcttcat cgtgaggccg atatcgtcga
     8101 gcccttccag taaaagatgc ttccggaatt cggcaatctc aaagcgatat tgcaggccgc
     8161 tcgcatcgct cacagtctgt gcaggcagat ccacggtgag ggaatagcct tcctgttttt
     8221 ccgctcggtg gaataactcg tcgatttgcg gttccggaaa aatgatgggc aaaatgccat
     8281 tcttgaaaca gttgctaaaa aaaatatctg cgaagctcgg cgcaatcagc acgcgaaatc
     8341 cataatcggc gattgcccac ggtgcatgct cgcggcttga accagaaccg aaacctgctc
     8401 gcgccagcag gatggaaact cccttgtagc gcggccagtt catgatgaaa tcaggattta
     8461 ctttttcatc ggccatgtag cgccaatcgt aaaataaaac cttgccgtag ccttccttcg
     8521 tttgcagctt caggaattgc ttgggcacca tttgatccgt gtcgacgttg acgcggtcaa
     8581 acggaagaac caacccagtg tgcctagaaa atgtctgcat gccttctccg ttgttaggtg
     8641 gcataggctt cagcctgtgg cttttcgtcc ttgcgaggtc aaaaccccac aggctgaagc
     8701 ctatgccaca aagcgcttat tccgttgctt ccacattcca atcacgcaca tccacgaaat
     8761 gtccggcaat tgctgcggcc gcagccatga tcgggctgac caaatgcgtg cggctgtctt
     8821 tcccctggcg cccttcgaaa ttcctgttcg aagtgcttgc agcgcgttct ccgggcagca
     8881 gcacatccgg attcatagcg atgcacatgc tgcatccggc atcgcgccat tcaaatccag
     8941 cgtcgcggaa aatcttgtcc aatccttccg cttccgcctt ttctttaatt gtcatcgagc
     9001 cgggaaccac gagcgctccc ttaagatttt tcgcaacatg ttttcctgca acgacgcgtg
     9061 cagcggcgcg cagatcattg atgcgcgaat tggtacacga accgatgaac acgcggtcca
     9121 cgggaatatc gacgagcgct gtgccgggtt tcaagcccat ataagtcagc gcgcgctcaa
     9181 cggctttctg atcgtcagga tcgcggaaag cgccgggatc gggcacgcgg tccgttactc
     9241 ccgcaaccat tccggggttc gtgccccacg tgatttgtgg cgcaagtgac gacgcatcga
     9301 gttccaccgt tgcatcaaat tttgcccccg gatcgctggc gagagctttc caattctgca
     9361 cagcgtcctg aaattctttt ccacgcggaa cgtacggtcg cccttccatg taagcaaacg
     9421 tggtgtcgtc gggcgcgatc attccggcgc gagcgccgcc ttcgatcgac atattgcata
     9481 cggtcatgcg cccgtccatg gacagcgctc gaatggcttc gcccgcatat tcaaagacat
     9541 ggcccgcacc tccgccgatg ccaatcttgc cgataatggc gagaatgatg tcctttgcgg
     9601 atacgccatg ggtgcgtttg ccgttgacca caatgtgcat ggttttcgat tcatactgct
     9661 gcatggtctg cgtggccaat acgtgttcca cttcacttgt gccaatgcca aaggccaatg
     9721 ttccaaacgc gccatgcgtc gacgtgtggc tgtccccgca cactagagtc tgtcccggct
     9781 gcgtcagccc aagttctggg ccaatcacat ggacgatgcc ctggttgtgg ctgaacatat
     9841 cgaagagcgt gatgccgttt tcgcggcaac tacgttcgag cgcatcgaat tgcccgcccg
     9901 cggccttgtc catgatcggc agattgcgat cttttgtcgg caccgagtga tccatcacgg
     9961 caaacgtcag gtcggggcgg cgcactttgc gccccgcggt cttcaggccg gcaaaagcct
    10021 gtggcgaggt cacttcgtgg atgaggtgac ggtcgatata gatgagtgag ggttcgccgg
    10081 gcgcttcgcg caccaggtgg gattcccaaa tctttcggta cattgttctc ggattcatat
    10141 ttgtgcatcc taatttagca ggccttggcg aactgtgccc ctttttcgat cagagccgca
    10201 ccgggcagaa agttagttgc atatttcttt ggacgcgccc ctgggaataa ttgattctcg
    10261 atacggaggt atttgaaccg gaaatgctgc tttgatcaaa tttataatga ttgggatagg
    10321 tataaatctg agttatgtgg ctaaatatgc tgctttcggg ttagcccgaa tttcgcacaa
    10381 atacgtttca gatcgccggg tgtttcgatg aatggcttca cattttgctg gtggcgaggt
    10441 tgaaggggca cggttcaccg agaacgttct ccgtgaacgg cattgccgtg cccctacggc
    10501 ttgcgcacaa ttcaccagga tccccagtcg aggtccgcca acatgaaatt gcgtgcacac
    10561 tgcttgtgag aagggcgggt tagcttttga atttgcggga tagcttcgca tttatttaaa
    10621 tttttagaaa accgccggcg gtacgccggc gccactgttg cagggtggtc actgggttag
    10681 tggagctggg tgcacgaagc tggggcgtga gtggtataat ttcgaaccag cggttgggcg
    10741 cggggctgtg aaacggcgag cacgacggga gtgttaaata tgggtatgca attgcgagag
    10801 tttgccaatt gggcgcgcaa tcgaaaaatt ttagcgacat tgctggtcac cgtaacccta
    10861 gcggttggta tgttgatcgg caccgtcatc tctggccacg tgggcgcggc gcatcctgcc
    10921 gccgctggcg caattggcgg aacgcctcta gccattcccg accctgtgca gctttccagc
    10981 gcatttgcct ccatcgttaa gcaggtgcag cccgccgtcg tgaacatctc caccacgcag
    11041 gtcatcgagc agcatggaaa tggaaaaggg cgcggccctt caccgcggaa tcgcggcggc
    11101 gaaatggggc ctccgggaga tcaggatcaa gaccagcagc cgtttcagga tttcttcaac
    11161 cgctttttcg atttcccgga tcagggtcct actgcagagc ggagtctagg atccggcgtg
    11221 atcgttgaca aaaagggttt catcctcacg aacaaccacg tcgtggacca ggccaccaag
    11281 gtccaggtca cgctaaatgg cgaacagacg caatacacgg ccaaagtcgt gggaattgac
    11341 gaagagaccg acttggcaat tttgaagatt gatgtcgggc acgatctgcc tttcgccaag
    11401 cttggcaact ccgattccgt gcaggtaggt gattgggcgc tcgccatcgg caatcccttt
    11461 ggatttttgc agggcagcgt aacggcggga atcatcagcg ccaaggatcg cggcaatgtg
    11521 ggccagcagt ttcaaaggtt tatccagacc gatgccgcca tcaatccggg taattccggt
    11581 gggccgctgg tcgatatggc tggccaggtg attggtatca ataccgcgat tattaccggc
    11641 ggacacggca atgaaggcgt gggcttcgct ctgccctcga acacagctac cggcgtctac
    11701 aaccagatcg taagcaacgg caaagtgacc cgcggctcga tcggtgtaag ctttacggaa
    11761 acgcagggca gcaatccgat cgtgttgagg gagcttggcg ctacgtatgg catcgtcttg
    11821 cagcgcgttg agccgggcag ccccgccgag aaagccggcc tgcaatcggg cgacgtggtg
    11881 accagtataa acggcaagcc ggttcacaat gggaatgagc tcgtaaatcc gattgcccag
    11941 actcccattg gcaattccgt gcgcgtaact tacttgcgcg acaaaaaaga acatgaggct
    12001 tccatcacgg ttgcggatcg cacgaagctc ttcccggatc gtgctgccgt tgccggaggc
    12061 aatgaaagcg ggccgacgcc tgccgagttc ggcttgcgcc ttgaagatct cgggaccgac
    12121 cgtgcgcggc gcgccggcta cgaaaatatg aagggtgcgc tggtcacggc tgttgatccg
    12181 gcaagtttcg ctgaagatgt cgggtttacc cgcggcgatg tgattacaga gctgaaccac
    12241 acatctgtga actccgtggc cgagtatcgc cgtgccgtct ccggcctgaa accaggacaa
    12301 gatgtcttgt ttaaggtgat gcgacggtca ggaagcgacc agttcgacac agtgtacctg
    12361 gcgggcgcgg ttccagcgcc tgagcagtag ctcgacaatt caaatttatt tggaccaata
    12421 gacaaccgat gatcaaacga atccggctga acctatttgg ggtcgcactc cttacgtgtg
    12481 cgactcccat tctgtttgcg gcggcacgcc ccgctgcggc tcaatcgtct ttttcctccg
    12541 cgaagaagaa aaagaaaacc gttactactg ccacaaagac aaaggggaaa tccaaaggca
    12601 gggttgcagc caggccgaaa gggcaggcag cccccacagc cgatcgcatc aaggaaattc
    12661 aatcggcttt gcaaaaagat ggttcctacg acggcgagcc cacagggaag tgggatgccg
    12721 caaccacgga tgcgatgaag aaatatcagg ataagaacgg cgtcaaccca accggaaaga
    12781 tcgatgcagt ttcactgaac aagcttggtc tgggttccga aacggccggc aaaggcgctc
    12841 ccatcccagc cgccaattct tcgactgctc cggcacccgc gtctaaaccg tccacttcag
    12901 tttcgcccac ttctgaataa ttcttcgaaa tagcgacagt tgatttttta ctttcggtct
    12961 gaatgaatag gattttcgcg gctgatgagg cggctcatgt ttacgttaat tccccaggag
    13021 aacgtgttat acgcgaagct catacttcgc gtgaatccga gatccaagtc taaataaggt
    13081 tttgggctga aggaaagacc tgtggtaaag ccgtcgtcgt aattcagcga ggcaggtccg
    13141 gtcgtcaaat gattcacttc gaacacacgg ccattgcttc ctccggagcc tatcgcacct
    13201 tttacaacgt cgcggctgat gatggtttgc gtcccccacg gcgcgatctt gtatccagat
    13261 acggtgaacg tgaacgagtg cgtcaaatcg atgtccgcgc ccgcctcgcc gtgcgcgaga
    13321 tagccgtagg atgtgaacgg gcgatgaaaa tattgagtat ccgtaaccgt gttggccacg
    13381 cccgcatcga ggaatgggga aagtaaggaa aaatcatggt cgatgcggtt attccaatcg
    13441 aacgtggcgt gtccggtgct gaatcctttg ctcgaattgc cggttggcgc ggaacccgtc
    13501 agagcggttg cgtaatttag aattgaataa ttccaggtcg caccgagcgt cacagaaaaa
    13561 tctccaacgc cttcgctggt cgtcactcca tttggcaaaa tggcgcggtt gaaataaaca
    13621 gggactccag cacccacgct gtagcggcca aaatgaaaag tcgcggacga acttagttcc
    13681 atttcctgtc cgctggatcc cgtgctgccc tcaaatgatt cgttccagtc aagcctgtcc
    13741 gttgtccttc ccgtcggaat actggagcct tgcgcaatcg cagaaacgct tgcagcgagg
    13801 aatatgacgg gaaaaatcag cgcagtcgta gttattttga ggttcatata ttcaggcaac
    13861 tctcctgaga atacccgctc acccaacggc aaaattcagc taacacggcc ttttattaat
    13921 gaatgcaatt ttacatttga taaggattgc caaagagtag gaaattcccc tcaggttacg
    13981 agagaattaa ccttggaggc gctcgcgcct gaattaattt gctgcgggaa gtgtgcgcac
    14041 gtactgaacc agattccaga tgtgttcatc cggttccacg ttgccgaacg ctggcatgcc
    14101 gctgaagcga attccatttc ttataatcca aaaaagttcg cgatcggaat actgtttgac
    14161 atccgcgagc gtaagatcag ctgcgcgagg atacatccag cgccctgcat ctgtgggctt
    14221 gttaccactc aggccatggc acgctgcaca ttctgttcca aataatttat cgccttcctc
    14281 gatgcttgct tgctggtttg ccgggggact cgaaatggga gcgcgactgg cgcggcgaat
    14341 gtaaaaatgc ctaagcttgc cggcaagaga aatttccgcg ttgtccggtg cagtcatcgc
    14401 gctgaggtta aattgccaca gtgcgagggc ggctccagcg ataagtaaac ccagcgcgat
    14461 gcaaaggacg atgagagcaa ttttcatatg caagtttcgg tacggcgtgc attggcgttt
    14521 taatttgcgc cccggcatgc ccgataaagc ctctccgaag ctttccatcc cgtttttccc
    14581 aggaaccaga tcagcctgat caggatagac gtcgggcata tggggcacgt gtaaggggtg
    14641 gaggaaccgt ccgcctgaac tggcggatcg gacggtctcc tcaggcgagt tgtaagatca
    14701 cattgttcag gatgctgctt caatgtttgt gacatggatg gtcttcgtcg ccttgtcgag
    14761 agtcccgctg acttttacat tggctccggc gaaatccgcg ggcttgctct gatcgtccaa
    14821 ttcatagacc gtctttgttt ttgaattata gagaacgtac tttccgccca tcttgatgca
    14881 gttctgcgtg cacatttttt tggtcatggc gtcgttcggg tctttgtcgc ccatgccttc
    14941 ctttttcaac atggcagcgt gcgaagcatt cttcgcgcac tggttatcca taatttcgcc
    15001 tgtgtacgtg ctgtcctttg gcgcggcaaa tatgactacg gataacaaga gggaaccggc
    15061 tactccaatt attgtttttc tcatgtgtca ttctcctaat ctctgtttgt gtttctattg
    15121 cggcaaagat ttaacttgcg gataactcct tttccagccg cgcatacagc ctcgcgctca
    15181 cggccagtgg cacctcgaag ggttcgacat cgagaacaat cctcagagat ttttgcgtcg
    15241 tatcgaacac cacacggcac ctgcggcact tggcaatgtg atcttccaga gaggcgcgaa
    15301 gttccgaaga agcctcgccg tccacataac tcgaaagatt tgccagcact tctttgcaat
    15361 ccatcatggc cataaaattt gtcctcactt tcccgccatg cgcatcatcc gcatggccat
    15421 gccagtaccg aagtccgttg cggcatgtaa aacttttctg cccagcgtgg gccgctgctc
    15481 gaacgtcgcg gccaaagatt cccgcagcag caaccgcgcc cgaagaaggc gcgatttcaa
    15541 cgccggaacc ttgattccca gagcctccgc cgcttcctcc gcgctcatct cttcgatatc
    15601 ccgcagcacc agggtctctc tgtaaatgag cggcagcgcg cgaatggctt cttcgaccag
    15661 acgccttact tcctgtttgc cgtatagctt ctcgggattg gttaaccacg gttcaaatcg
    15721 gccgggaatg gatttgcact ctacttcggt agattcttcc aaaggcacag acgacctgcg
    15781 gctttgtctt ttctgaatcg actcattgac cgcaattcgg gtcaaccaag tcgtaaattt
    15841 ggaatcgccc cggaaatgcc caaggttacg ataggtctta ataaaagttt cctgcatggc
    15901 gtcttccgcg tcttcgacat tccccagtac agcgagggtc actcgaaaaa cgcgtttctc
    15961 atgccttcga acgagagttt caaatgcctg gcggtctccc gccaaagcca gacggatcag
    16021 tgcgaggtct tcgtcgcttg gaacttcact cgttttggga aattcattca ttcgcatcca
    16081 tgagactggc cccgccatat aaaagattca atgatcgccg cttaatatcg taaagcagaa
    16141 gcatggtcga gtaggcgaaa ctgagcgaaa aatggagcgt gatgggtgtt taatgatctg
    16201 cccattacgc tggctgggaa gcccacattg acgagattgc cgtaaaaaag atagcctctt
    16261 gagaaagaac cccctaggag gaaaccgtat gcgtcgtcgc gcgtggctcc aggcaatggg
    16321 tataattctg atttccgcgt cagcgaatgt ggcgtccgct gcgcaaaagg gaggcggccc
    16381 gccaatgccg ttcgttcctc tgcgcgaacc ccactttgta acggcatcca gggcggattt
    16441 tctcaaggca gacgatcgag ttgtgggagt cagcgaaaat ggcgtgacca aggcctatga
    16501 gcctagcgtg cttgcgtttc atcacgtcgt gcaagacaac ttgaaggata tgccgatcat
    16561 cgcgacgtgg tgcgcgctct gcaatactcc gcttgtgtac agcagcgtgg ttgatgggaa
    16621 gaagctcacg ttcgaaagag caggcaatcg aggaaataat tttttcatgc tcgatacgga
    16681 aaccggaagc cactggcagc agatcggcgg cgattgtttc gaaggccccc tgaaagggaa
    16741 acgattgacg atggttccgt tcctttacac cacgtgggga gagtggcgcg agcaacatcc
    16801 cgacacgtta gtgctggtgc cggaggccgc gtataaagcc ggttatgaat tcatggagaa
    16861 gagccgcgtt tccgtggttc cttacggcag caataagaag cccgatcgcg agctggtgcg
    16921 cgatttcgat acgcgcttgc cgaattatga gcaagtcata gggattgaaa ttggagatct
    16981 gcacaaagcc tacccgcttt cagcgttaaa aaaagaaacg gtggtgaacg ataaagttgg
    17041 ctccatcccg attcttcttt tgtacgcagc gggcagcgac acgacgacag gattttctcg
    17101 tattctcgga ggccgcacac ttacgtttcg tgccgctgcc ttaggctctt ttatggataa
    17161 tgagaccggc tcgaactgga gcgtttacgg aaactgtttg gacggaaaac tgaaaggcca
    17221 gaaattggaa cgcgtcattc ctcagcccgg ctcgtggttc gcttgggctg aatttcatcc
    17281 agacacggaa atttatcaat ccggttcgca ctaacttcaa ttgagcaccg ggcgggtgat
    17341 caccgcaatt tttccgttaa cgatctggat atcttcaaca cagcgcatat tcggtagtgt
    17401 cctaatttga aaatttctcg aatttaggag ggccgagttt caactcggcc atttggtttg
    17461 cctttcgtcg cgggcttcag cccctgaagc ttcaggggtt aaaaccccga tcaaaccaag
    17521 ctcgtcgtcg ggactgaagt cccgacctcc taaggaaccg caccacgcga attcttttca
    17581 aattaggaca ccaccgcata ttcagcgggt ttgaccaccc catgaccctc tgctacattg
    17641 aaatcacgcg cggaaaaagt tcccgcgtta agcaatttac gcgttctgcc aaccttagtt
    17701 gtaaagaaaa acctcacatc gaccgcggga ggcaacatga cgaccagcgc catttccgga
    17761 agccacaaaa actttattaa cggcgagtgg gtggaagcgc ggcgcggagg cgtatttgaa
    17821 aaccgcaatc ccgcgcgcac gagcgagctt gtcggaattt ttccgtcgtc aacctcggag
    17881 gacgtggacg atgccgtgga cgccgcgaaa aatgcgttta aacactggcg gctgtttcct
    17941 gcgcccaggc gcgccgaaat tttgtatcgc acggcggaaa cactggtgaa gcgcaaggaa
    18001 gatttagctc gcgaaatgac gcgagagatg gggaaacctc tgtccgagac gcgcggagat
    18061 gtgcaggaag ccatcgacat gacttactac atggcaggtg agggccgcag gcttttcggc
    18121 caaaccgtgc cttcggaatt gccaaataaa tttgcgatgt ccgtgcgcga tccgctcggt
    18181 gtggtgggaa tgattacgcc gtggaatttc cccatggcca ttccttcctg gaaaatcatg
    18241 cccgcgctga tctgcggcaa cacggttgtg attaagccgg cggaagacac gccgctatcc
    18301 acttacaacc tcgtgcaaat tttgattgag tccggcttgc cgcccggagt tttgaacctc
    18361 gtggcaggaa tggggcctga cgctggagcg ccgctgatca cgaatcccgg agtttcggcc
    18421 gtgagcttta cgggctcaac ggagaccggc cgcatcgtga gccaggcatg cgcgcccaca
    18481 tttaaaccct gccacctcga aatgggcggc aagaacatca tgatcgtgat ggacgacgcc
    18541 aaccttgaac tcgctgtcga aggcgcggta tggggcggct tcggaacgag tggccagcga
    18601 tgcacagccg ccagccgcgt cgccgtgcac tccaaaattt acaaggaatt tttgaaactg
    18661 ttcaccgagc gcacacgcgc gctgaagctg ggcgacgggc tcgatcccca aacggatgtc
    18721 ggcccagtgg tcaacgaaac gcaactcttt accgtgatga agtatgtgga aatcggcaag
    18781 agcgaaggcg cgcatctggc agtcggagga catcgcgcgg aatctggcgc tctggcgaag
    18841 ggctggtttc atgaacccac aatttttagt gacgtcgatg caaaaatgaa aatcgcgcaa
    18901 gaagaaattt tcggccctgt cgtggccatg atgccgtttt cttccctcga aggagccatt
    18961 gagattgcca acaacgtaaa atatggtctc tcggcttcca tctacacgcg caatgtgaat
    19021 caggcttata gcgcgatgcg cgatctgcaa accggaattg tttacgtgaa tgctccaacg
    19081 attggcgcgg aaattcaatt acccttcggc ggaacaaagg gaactggcaa tggtcaccgc
    19141 gaagctggaa cagccgcgat tgatttttac tccgaatgga aaacgttgta cgtggattat
    19201 tcagacaagc tgcaacgcgc ccagatcgat actccttagc ttccagtggc tcgggcttca
    19261 acgtgtgcca tccgctgtag cccgcctctt cagaggcggg ggtttagatt ccgcacgtcg
    19321 accaagccca ttgggatata aaccccgacc tcgaatctct tctctaataa cgataataag
    19381 gaggctggct gcaaaggcgg ggcaaaaaat ggcggcgtct cacccgcgct ggttgaacac
    19441 acacgttttg tataatttga cgctgaggcc cccaacgcat aaaatcaact cagtacgggg
    19501 attttagatc gtaagaccca tgtcggaaac gactccaaat agcgaacgca atcggcagat
    19561 tcttaccgcc gtggtgcgcg cttatattga gacgggcgag cccgtttctt cccggtcgat
    19621 atcgcgtcgg cacgtagagg ctttgagttc tgcgaccatc cgaaacgtaa tggccgatct
    19681 ggaagaagaa ggctatttat ttcagccaca tacatcggct ggacgagttc cgacagccaa
    19741 cgcttaccgg tttttcgcgc aacagatcgt caccggcgca actctaagcg ctcaggacga
    19801 agatttgatt cgcagtgaga tctcaggcgc gtcctcgggc gaggaaatcg ccgaacgtgc
    19861 cggacacgtg ctggcggaaa tttcaaaagg gcttggaatt gtcgtatcgc ctcccatctc
    19921 aaaaacagta ctcgagcatg tcaggttcgt tctcctgcca gatagccgca tcgtcgttgt
    19981 attggtgtcc acgggaggaa acacgcggga taaagtgatt cgcccggaga aagtattcag
    20041 ccagcaggat ctcgatcaca cagctgaata tttgaatcac cattacactg gatttttact
    20101 ggaagcgatc cgcgccgatc tgcaaaaacg gctcgcgcag gaagaagagc ggtacgcgat
    20161 gctcgcgcgg aacgcgttgg aactgtgcga tcccggcgtg atggggcagg gttcggcgca
    20221 acgcatatat attgaaggcg ctgcacaaat ggccgccgct ccggaatttt ccggcccatc
    20281 gcaaatcagg gatgtgctcg aggcgattga agaaaaacat agaattattg cgctgctcgc
    20341 aaactgcatc gacacgccgg atcctgtgca cgtgcaaatc ggcgtgaagg gaatggaccg
    20401 cgccggcgaa catctggcgc tgatcagcgc tccgtattca tttcaggatc aatcgcaggg
    20461 caccgtaggt gttttgggcc ccatgcgaat gcagtatgag cgcgcgatca ccgcagtggc
    20521 atttgttgcg cgcgccttca gcgaaacttt ggatcggagt tagaagccgt ggcagaagcg
    20581 aaacgagaag aaaaaaaagt catgcccatc gacgggaatc caagcgagca agataacgaa
    20641 tcgcaaaata ttgcggaaga tcctatttca gtggatccgc aggcaggcat tgattccgcg
    20701 cttgccgcgg aagtgcaaaa gctgaaggcc gaaaaagaag atttaatgca gacgatgatg
    20761 cgccgccagg ccgacttcga gaattttcgg aaaaggactg agcgcgaccg cagcgaagaa
    20821 gccaagcgcg gggtggagcg gctggttctc gatctgattc ccgtgatcga cgcctttgac
    20881 cgcgcgttgt tggcgcatga cgatccgcgc tatgaggaat atcgcaaggg aattacgctc
    20941 attcgcaagc agctttggga cgcgattgcg aaacatggcg tcctgccgat tgaagcggcg
    21001 gggaaaattt tcgatccgca ccatcatcag gccatcgagc gcgtggaatc cgaggaatat
    21061 cctgacggca cgattgtggc cgtatttcag gatggttata tgttcaacgg acgcgtcttg
    21121 cgtccgtcaa ttgttcgagt agcagtgcat cctgacgagg gtgaaaattc cacatccaat
    21181 gaaaattagg ccaatcaggc gacccagccg atgagtgaaa aacgcgactg ttatgacatt
    21241 ttaggaatac ctcgcacggc cggtgcggat gaagtgaaat ctgcgtaccg taaagctgcg
    21301 ctcaagtggc atccggaccg caatccgcat acaaaatcgg aagcggagga aaaattccgc
    21361 gaagccaccg aagcctactc gattttgtcc gatgttcaaa agcgcgcaac ctatgatcgt
    21421 ttcgggtatc agggattgtc gcgcggcgga ttcgatggcg gcgtcaatca aaccattttt
    21481 gacgaatttc aagacatctt cggcgacttc tttggatttg aagatgtgtt tggaggccgg
    21541 ggcggcagtg gacgtgggcg cagccgatcg caacgtggcg ccgacctgcg ctatgacatg
    21601 aagttgagct tcgaggaagc cgctgcggga gtaaatacca agatccgaat tccgcgacag
    21661 gaattttgcg aagtctgcaa cgggacaggc gcgaaggcgg gaaccggagt tgtatcctgc
    21721 gccacgtgcg gcggccgcgg acaagtacat tatcaacagg ggttttttac gatttcgcgc
    21781 acgtgcccgg aatgccgcgg cgcgggacaa atcgtgcggg aatcgtgccc tgattgcaaa
    21841 ggcaagggac gcatcgagcg aagccgcaca atcgatctga gaattccgcc aggcgtcgat
    21901 tccaatacgc gcttgcgaat tccaggcgaa ggcgaacctg gcggcaatag cgggccggct
    21961 ggcgatttat atgttgtgct ggaagtcaag ccgcatcctt actttgagcg cagagatgcg
    22021 gacctgtatt gcactgtgcc tgtttccatc gtgcaggcgg cattgggaac cgagatagtt
    22081 gtccccggat taaacggcga agaacacgta aaaattcccg aaggaacgca aaccggagaa
    22141 attattcggc tgaagggaaa aggcatgccc gacccgcacg gtggcggcaa gggagacttg
    22201 tacgtgaata ttcacgtgga aatccccacc aagttgaccc gcgagcagcg ccgtctattc
    22261 gagcagcttg gcacaacctt gcccgtggaa aatcgccccg cgcatcgcga ctcttcactc
    22321 ttcgaaaaag ttaaagatat tttcgggtaa tcgaatagct tttatcatgt ctctgtggcc
    22381 ctcctctttt ctctgtgtcc tctgtgttca gctttgcgaa tgtaagttca aaatcccaac
    22441 acagagatag gaggagaaca cagagaatcg ttactcagca tcagagcatg tgatgcgcga
    22501 gtcttcttga tttccagttg ccgtaactgt aatcgtcagc gtgaaatgca gagcggtgca
    22561 aaaaccggcc aagggcggtt tggcatcgga ttgatatacc atagtgggac atgcgaagac
    22621 gattttttgt ggatgagttt gaaaatggat ccgccatttt gcgcggtgaa ggggcgcatc
    22681 atttgggccg cgtgttgcgg gctgagccag gtcaactcta tgagctgagc gacgggcagt
    22741 gtctgtggct cgcgcggacg caaagtgtgg ggcgtgacga agtacagttc acacttgttg
    22801 aacaacttcc ggcaacgact gcgcccgttc gaattgattt gcttctggcc atcgttaaat
    22861 ttgaccggtt cgaatgggcg ctggaaaaag cgacggaact tggcgctgac gaaataacgc
    22921 cacttgccgc ggatcgcagc gaaaaaggtt taattgccgc ttcgggaaaa cgcgccgaac
    22981 gatggaaaag aattcttggg gaatccgcgc agcaggcccg gcgcctgcat gtccccggac
    23041 tggcagctgc tgcgaaacct cgtgaggcgt ttcgggctcc ggcagcgcaa ttaaaattaa
    23101 tgttttccga acgtgccggc gcacagccgc ttcgcgatgt gcttgatccg gtcgccgtcg
    23161 cagttcgaga aaatgaaacc gcgtgcgtgg cgattgcgat cggtcccgag ggcggctgga
    23221 cggacgcgga atttgccgcc tcaatagaat gcggctgcgt ggaagtggca ctcggaacca
    23281 atattctgcg cacggaaact gcggtctgcg cggcgcttgc ggcagtgcaa tacgcgttcg
    23341 gcgcatttag acaaaagtaa tgctaacggt ccgcaggttt atgtggcata ggcttcagcc
    23401 tgtggggttt agtttgtgca aggactaaat tccacaggct gaagcctatg ccacaaaatg
    23461 ccgaaccgtc gtctgctgaa ttttggaaga atttaaagga gtatcctgaa aaaaggacaa
    23521 gccgggttgg actggaacgc aaatctgtac aacgcgaagc acgacttcgt gtggaagttt
    23581 ggcacagacg cggtttcgaa gcacaaagaa tttatccaaa gaaatttatc gaaagtgggg
    23641 aagaaaaact ccgcccgaaa ttatttcgcg acggccggtg gtgggcggat taccgcaggc
    23701 tgcgtcttgt ggcatacaaa taaagaataa tatccgcgcc acgaatcaaa tgttggggtt
    23761 gcaggaatga aagatgcggt gaagcgctca ctcgctgtat aattcgggca gcagcccgat
    23821 ggaactggac agcgattccg atcgcgcaat ttttccgagc ttctcttcca gtagcgcgca
    23881 tcgaagcagc atggtataaa attaattgtt tacacgtaag gaacatggag ccgaacgccg
    23941 aaattagcac gtccgtggcg cccggagcgg cgcaaccgct gcggcgcgag cttcgaagtt
    24001 gggggcgcga tctcgccatt gcgctcagcc tcgctgtcgt tattatcatt tttttctacc
    24061 aaccggtaaa agtggaaggc acgagcatga ctcccctgat ctcggatcag gagcgaattt
    24121 tcatcaataa gtttgtttac agatttgagc caattgagcg cggcgatgtg gtcgttttct
    24181 ggtatcctct tgaccgctcg aagtcgttca ttaagcgcgt ggtgggattg ccggatgaca
    24241 cggtcgaaat tcgtggaggg caggttttcc tgaacggcag acttttgccg gagccttaca
    24301 ttccgcccga gtcggacgat tacggcacgt attcgccgat gcaagttcca aagggcgagt
    24361 attttgtgat gggggatcac cgcacaagct cgaatgattc acgaatcttc gggccggtgc
    24421 cgcgaagatt tatctacggc aaggcggtgt tcgcctattg gcctgtggat cacttcggat
    24481 cgatccatgt gtttgcggaa tcggcgaaat aacccgcctt cgcccaaagt cggctatggc
    24541 gggctggccc gccgaagccg gtcttgggcg aagatggata ggcatgatgc aagaatcatc
    24601 tgaatgtatc gatacgcgcg acggcgattt ggccagggcg cgagcaaaga gaggatccac
    24661 tgtcggaagc agaagcacga aagggaatac ttgcgctcga agacgggcga attttttatg
    24721 gacgcgccgc gggagctgtt acccggcggg gcggcgaagt tgtttttaat accagcctga
    24781 ccggctatca ggaagttttc actgatccga gttatgcagg ccagattgtc tgcctgacat
    24841 atccccacat tggaaatgtc ggcgcgaatt ccgaggatga ggaatcttcg cgtccacaca
    24901 tcgagtcgct gatcgtccgc gaattttccg ccgtgtcctc caactggcgc tcggctgaaa
    24961 ccgctgagaa ttatctggtg cgaaacaata tcccggtgtt gtgggatatc gatacacgcg
    25021 cgctggttct gcacattcgc aaacttggcg cgctgcgcgg cgtcgtgtcc acggacggaa
    25081 cgcctgctga aaaacttctg gcggaagctc gtgcgctgcc atcgatggcg ggtctcgagc
    25141 ttgcgagccg cgtcacttgt gaaaagcaat acgggtgggg aaaaggttct attgaattga
    25201 cggttccacc ctggcaggaa gcggtcggcg aatcggaagg tgagaaaaaa tcgccgaaat
    25261 tccgcgtggt cgcttacgac tacggtatta agcagaatat tcttcgcttg ctgaccgatc
    25321 accaatgcga agtcgtggtc gtgccggcga aaacttccgc ggaagatgcc cttgctttaa
    25381 aacctgatgg cgtttttctg tcgaacggtc cgggcgatcc tgagcctgcc acctacgcaa
    25441 ttcagacgat ccgcgacttg cttggcaggg tcccggtgtt tggaatttgc ctcgggcacc
    25501 aactttgcgg ccttgcgcta ggcggcaaga cattcaagct tcgctttgga catcacggat
    25561 cgaatcaccc ggtgaaaaat cttctgaccg gacaagtgga aatcaccgcg cagaatcatg
    25621 gattctgcgt ggatcccgat tcgctgccgg cgagcgacgt cgaagttacg cacctgaatt
    25681 tgaatgacca cacgaatgag ggatttcgac accgcacgct gcctttattt tcggtgcaat
    25741 atcaccccga ggcgtcgccc gggccgcacg attcccatta cttattcagg caattcacag
    25801 acacaatgaa aagctggcaa tcatcaggcg caacacggcg cgggcaggcg aaggcttagc
    25861 cgcacatgcc gaaacgcaca gacatcaaaa aaatcatgat tatcggctcc ggccccatcg
    25921 tcatcgggca ggcctgtgag ttcgattatt caggaacgca ggcgtgcaag gccttgcgaa
    25981 gtgaaggcta cgaagtggtt ctcgtgaatt ccaacccggc gaccatcatg accgatcctg
    26041 aaatcgccga ccgcacatac atcgagcctc ttaccaagca atatatcgaa aaaattattt
    26101 ccatcgaaag acctcaggct cttctgccca ccgtgggcgg acagaccgct ttgaacctgg
    26161 cggtggaact ggcggagggt ggcatacttg cgaagtataa cgtggagtta attggcgcct
    26221 ctctgcatgc gatcaaggtt gccgaggatc ggcttctgtt caaggacgct attcgcaaag
    26281 taggactcga cgttccggca tccgcactga ttaacaatgt gcaggacggt ttaaaacttg
    26341 ccgaccagct tggatatccc gtcgtcatcc gaccttcatt cactttgggc ggcacgggcg
    26401 gctcgatcgc ttacaaccgc gaggaatttt cggaagccat ggcgcatgcg ctcgatgcga
    26461 gcccagtgca cgaggcgctc gtcgaagagt ccgtgctcgg ctggaaggaa tacgagcttg
    26521 aagtgatgcg cgattatcgc gacaacttcg tggtcatttg ttcgattgaa aatttcgatc
    26581 ccatgggcgt acacacgggc gattcgatca ccgtggctcc cgcgcagacg ctgaccgaca
    26641 aggaatacca gcgcatgcgc gatgcggcgg ctgtgatcat tcgcgaagta ggcgtcgaaa
    26701 ccggcggatc gaatattcag ttcgcgctcg atccggctac cggacgcatg atcgtgatcg
    26761 aaatgaatcc gcgggtttcg cgcagctccg cgctggcatc gaaggccacc ggatttccga
    26821 tcgcaaaaat tgcggcgctg cttgcggtag gaatgtcgct cgacgaaatt cccaacgaca
    26881 ttacgaagaa aacgccgtcc tgctttgagc cgaccattga ttacgtcgtg gcgaaaattc
    26941 ccaagtggca atttgagaaa ttcgcaggtg cggattcgac gctcggcacg cagatgaaat
    27001 ccgtcggcga agtgatggcg attggccgca catttaagga agccctgcaa aaagggattc
    27061 gttcgctgga gcccagttcg ccatggcgcg cccccgcgga cacgcccgtc tcgcttttgc
    27121 gcgaaaaaat tgcgacgccg cgccccgacc gcattcactg gctgttcgtt gcgctggagc
    27181 gcggaatcag cgccaaggaa gcaatcgatc tgaccaagat cgatccgtgg ttcatcgagc
    27241 agatgctgga aattattgcg gtgaacaaac gattggcctc cgtcaccatg gaaaccgtgc
    27301 ccgaagattt gctccgcgaa ggcaagcgca ttggcacgag cgatgaacaa ctcgcgcaaa
    27361 tgtggaagac cactcccgcg gccgtgcgcc atcgccgggc aaaggcgcat attgctcccg
    27421 tgttcaagcg cgtcgatacg tgcgccgcgg aattcgagtc cttcaccccg tacatgtatt
    27481 ccacgtacga agaagaagac gaatcgcaac ccactgatcg cccgaaaatc gcgattctag
    27541 gcagcggccc gaaccgcatc gggcagggaa ttgaattcga ttattgctgt tgccatgctt
    27601 ctttcgccct gcgcgaagat ggctacgaga cgatcatgat taattgcaat ccggagacgg
    27661 tgtcgacgga ttacgacact tcggatcggc tctattttga gccgctcacg ctcgaagacg
    27721 ttctctccgt cgtggatcgc gaaaagccgc aaggcgtgat cgtgcaattt ggcggacaaa
    27781 ctccgctgaa cctggctctg gaactgaagc gaaacggtgt gcccatcatt ggcacggcgc
    27841 ccgagtcgat tgacctcgcc gaagaccgca ggcgtttcgg aaaactgctg gatgaattga
    27901 aaattccgca gcctaagaac ggaacagcgc tcgtgcctga agaggctgcg cgaggtgccg
    27961 gcgaaatcgg ttttcccgtg ctcgttcgcc caagctatgt gctcggcggg cgcgcgatgg
    28021 tcatcgccta cgatttgaat acggtgcagg aatatgtggc gcaagcggcg ctcatgggcc
    28081 ctgccaaacc ggttttgatc gatcaatttt tggaagaagc gacggaagtc gacgtggacg
    28141 cgctggccga tggggaggac gtgatcattg gcggcgtaat ggagcacatc gaagaggcgg
    28201 gcgtgcactc cggagattct tcctgcgtgc tgcctcccgt cagcctgtct cccactgtgc
    28261 tcgcaaagat tcgcgattac acgaatcgcc tggcgaaggc tctgcaagtg gtcggtctga
    28321 tgaatgtgca gtatgccatt caacgtgaca cggtctatgt gctggaagta aatccgcgtg
    28381 cttcgcgcac ggtacctttt gtcagcaagg cgacgggcgt gccgctggct aaagttgcag
    28441 cgagattgat ggcgggacgc aagctgcgtg aaatgaattt gccggtcgtg attacggacg
    28501 gcgtagcgga aattgcggtg aaggactatt actccgtaaa atcgcccgtg tttccgttca
    28561 acaagttccg cggcgtggac acgattcttg gtcccgagat gcgttccacc ggcgaagtga
    28621 tgggggtcgc caagagttac ggccaggcat ttgccatggc gcagctcgcg gcgggacagc
    28681 gattgccgcg gaaaggctcc atctttttga gcgtaagcga tcatgacaag cgccactgcg
    28741 gacctctcgc gaaagaattg cacgcattgg gattccggtt aattgcgacg cgcggcacgg
    28801 cagcggcgct cgaagcggca ggcgtt
//
LOCUS       DASZMB010000208        18774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2683625, whole genome shotgun sequence.
ACCESSION   DASZMB010000208 DASZMB010000000
VERSION     DASZMB010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 18774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18774
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2683625"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(327..1601)
                     /locus_tag="VG272_09510"
     CDS             complement(327..1601)
                     /locus_tag="VG272_09510"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HWF13957.1"
                     /translation="MTDKLLQLMPLEGFKILLTLFLCFLIGLEREERRAEVHHYGFGG
                     VRTYPLIGLIGYAMALLSGAQLLPLTFGFGVIGAFLWLSYKHKLETYNEAGVTTEISG
                     LVTYVIGALVSRDQFWIATTIAVISMLLLELKEYLEGLSTEIPAIEILTFTKFLLLTF
                     VILPILPNQEYGPFGINPFRSWLIVVAISGVSYGSYLLEKLSKGKGGVRLAALLGGLY
                     SSTFTTVVMGKQAKTAGSPHTYAGAILIASGVMYIRFLALIGIFNLELMKHLSLPFLI
                     LGAIGVAGGWAWSNLSDDQEETGAKPAYLTKNPLELTSALLLGLVFVVVLVATNFAMS
                     RLGSAGIYGMAVISGLAPVDPFIMGLTQTAGKVTTMGLAISGIVVAAASNNFAKAFIA
                     LFVADSKTGRQSLGLMLALTALGLVPLLWHGL"
     gene            complement(1728..3287)
                     /locus_tag="VG272_09515"
     CDS             complement(1728..3287)
                     /locus_tag="VG272_09515"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HWF13958.1"
                     /translation="MSLLTIKVRPRSSTFLFATIAALVYCLLFAGRAIAQSGGGPVGA
                     PTFKVLRAMAGSSGKESNGRLILDDPRSVFYIGQDHKIIVYFEWEGPAGLHKFEGIWK
                     NADSKVTLISDFQYVAPGKQFSGYWSMLLSGSETPGLWTLEAHIDGESAGDLSFQLIA
                     GPGAVTPVAPAPPRQPLSTGDLYKRLADVSVFVDKIDTHGKAVARGSGFYLADGQLIT
                     AFQNVDGATKLRVISPDGSAQEVTAVRSWDRWKDWAILAISGAKVNGLTRAAPKSWSV
                     GSICYFLQTSSGSGRVIADGTIVGQNTFPRAGERMNIAESPSRGAIGSPLTNEFGDVV
                     GMVGGSLAPGTDLLGSYMLTMAGNGLGGTSYIRDGLAVPINLIPASSSQDQTNLEELT
                     RKGQMVPLINSEQKIIIAELALTLEKGRGVPTPSNPSQQFSHKDQKIWVYVNWNENIS
                     FKGTAAMAVYDVDNHALGQSNPVKVSLHSGALSSSTWEIPLATLPVGIYRVDVSLGED
                     IVWRKFFRIAD"
     gene            complement(3455..4870)
                     /locus_tag="VG272_09520"
     CDS             complement(3455..4870)
                     /locus_tag="VG272_09520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HWF13959.1"
                     /translation="MGKVTGFKEYQRETPARRPVAERIHDYFEVYQPFSEEKVRTQGA
                     RCMDCGIPFCHTGCPVNNIIPDWNDLVYKDRWKDAIRVLHATNNFPEFTGRICPAPCE
                     AACVLGINEPPVTIKLIEKTIVDRAWEEGWILPEPPETRTGKRIAVVGSGPSGLAVAQ
                     QLNRAGHDVTVFEKADRIGGLLRYGIPDFKLEKRVVDRRVEQMMAEGVQFQLNAHVGT
                     SIPAQDLRKEYDALVLSGGAEHPRDLNVPGRELKGIYFAMQFLPQQNKVIAGDTIPEQ
                     ILATGKRVVIIGGGDTGADCLGTCHRHGAKSIHQFEIMPMPPAERAPSTPWPLWPLQL
                     RTESAHEEGGNRDWALNTAKFTGDDQGNVKQLHAMRVGPPPKFEPRPGTELVMEVDLV
                     LLALGFLGPVRNGMIEQLGVNLDQRGNIAAGADYMSSVPGVFAAGDMRRGQSLVVWAI
                     AEGRKAAASVDKYLMGNTTLS"
     gene            complement(4863..9455)
                     /gene="gltB"
                     /locus_tag="VG272_09525"
     CDS             complement(4863..9455)
                     /gene="gltB"
                     /locus_tag="VG272_09525"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HWF13960.1"
                     /translation="MSELYPFRGVPPAQGLYNPQHEHDACGIGFVASIEGRKSHDIVT
                     KGVQILINLAHRGACGCDPQTGDGAGILIQIPHAFFEREAVNMGFSVPGPGEYGVGMV
                     FLPVDTHDRLICEGIIEKIVREEGLAVLGWRDTPINGNTIGRLARASQPYIEQIFVRS
                     APGMDQDSLERKLYVIRKRTEAEILATDLKEKEFFYIPSLSSRTIIYKGLLLAPQITD
                     FYKELLDPDVTSALCLVHQRFSTNTFPSWKLAHPYRYICHNGEINTLRGNMNWMYARQ
                     SVLQSPLFGDDIKKLLPVITAGGSDSAMLDNAVELLTLSGRSLPHVMSMLIPEAWDHD
                     DTMPEDIKAFYEYHASLMEPWDGPAAVAFTDGRVIGAKLDRNGLRPGRFLVTNDGLVV
                     LASEAGVLPIKPEDVRLKGRLAPGRIFLVDTEQKRLITDEEVKKHLAARRPYAQWLKE
                     NQITLDHLPSPTHVQPTDHDTIRMRQRAFGYTDEDLKTILLPSALNGEEPVGSMGIDT
                     PLACLSDKPQMLFNYFKQTFAQVTNPAIDSIREGLVMSLNSYIGSEGNILEETPKNCH
                     TLKLRHPVISNWRLEKLRRVSWGSFLATSLPMLFRVSGGEQQLEESIENLCRRASLAI
                     KSGYTLLILTDRGVDHEYAPIPSLLAMSAVHNHLIREGTRNQVALIVESGEPREVMHF
                     ALLLGYGASAVNPYLAIETLEDLHRSGAFPADTTWDKVFKSYMKSIDKGLLKTFSKMG
                     ISTLQSYQGAQIFESIGLDKSLVEKYFTGTSSRIQGVGIEVLAREAIMKHDFAMQPPH
                     LSDTELRVGGEYQYRVRGERHLLNPTTVSKLQHAVRQEKFESFREFADIVNQQNRDLQ
                     MLRGMFDFKPNGPAIPLDQVEPASEIVKRFATGAMSFGSISKEAHETLAVAMNRIGGK
                     SNTGEGGEDEARFTPGANGDLRRSAIKQVASARFGVTTNYLINADDLQIKIAQGAKPG
                     EGGQLPGHKVDDVIARVRHSIPGVGLISPPPHHDIYSIEDLAQLIFDLKNVNPQARIS
                     VKLVAEVGVGTVAAGVAKAHADVILISGYDGGTGASPVSSIRHAGIPWELGLSETQQV
                     LVLNDLRSRVRLQVDGKLQTGRDVAVGALLGAEEFGFATTPLIAMGCIMMRKCHLNTC
                     PVGVATQDPELRKKFSGQPEHVINFLFYVAEDLRLIMAELGFRKMDEMIGRVDCLVQR
                     KDVDHWKAKSIDLSSVLYNPPMPSHVGRRCTQAQDHGLSDALDGKMIEQATDAIERLT
                     PVSFNLPIRNVHRTVGAMLSGRIARKHGSAGLPDETVRIQFHGCAGQSFGAFLSAGVS
                     LTLEGEANDYVGKGLSGGRIVIYPPRNRNSQFVPEENILVGNVCLYGATSGEAFFNGM
                     AGERFAVRNSGATAVVEGVGDHGCEYMTRGLVVVLGKTGRNFAAGMTGGIAYVLDQSG
                     EFAEASCNRADVDLEPVTDTKDIEVLHRLVTRHAELTDSPQGQRILKHWDSALSKFVK
                     VFPHEYKRVLGVPRIPATVVAAQAGSAQAQGQALRG"
     gene            9826..10776
                     /locus_tag="VG272_09530"
     CDS             9826..10776
                     /locus_tag="VG272_09530"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HWF13961.1"
                     /translation="MDFDQLTTFIEIAKLGSFSRAGQKLFRSQPAVSAQVRQLEQEYG
                     EKLFDRGRKSVRLTSAGETLFEYAVRMANLRAESQRAVADKATTPRGVLALGANEATC
                     LYVLPDIFAEYHRLYPSVQISIYRNFSHKVLQKVEDGSIDVGIVTLPAKSASLKFHPI
                     FRDRVMLMVSPRSPLAKLKSVRMKDIADQPLIFPRTGYTRQLLDKHFRPYKPQLQITM
                     ELTSVGMIKCFVAANLGVSLISSSFARNEVRAGEVKLIPIQDVELWRELGIVYRRDRT
                     LPRSSSAFIQLVRQRAAENPDLEIPPRKAARASSEAAPLP"
     gene            complement(11238..13205)
                     /locus_tag="VG272_09535"
     CDS             complement(11238..13205)
                     /locus_tag="VG272_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF13962.1"
                     /translation="MRMPGGGLGSQHGMAMRVERGHSARRGRGDPSDASKKKPVRILD
                     QLPQIWALMRPRRGLLGIGMVLMAINRVSGLALPFTTKYLVDNVMIHHQTRMLVPLVG
                     GVLLATILQGVTSYTLTQTLSKAAQRMITELRRKVQEHVGRLPVAYYDENKTGALVSR
                     IMSDVEGVRNLIGTGLVEFLGGLLTAFIALYMLLRISPVMTGLAAGLLLVSMFGLRKA
                     FGILRPIFRERSKINAEVSGRLTESLSGVRVVKGYHAEDREADVFTKGVQRLLDNVIK
                     SLTTMSMMSFSTTALGGVVGALVMLVGSHQVLQGKITVGQFMSYTAFLAFMIAPVFQV
                     VGIGTQLTEAVAGLERTKEVLDERPEDEDPRRTVTLPDIVGELAFDDVNFAYEPGKLV
                     LQDVSFLSKPGTVTALVGSSGSGKSTTIGLVAAFHVPLSGRVLVDGVDLSTVRLDSYR
                     TRLGVVLQESFLFDGTIRENVAFSRPYATEEEILAACRIARVDEFAERFPETYDTIIG
                     ERGVKLSGGQRQRVSIARAILADPRILILDEATSSLDSESEMLIQEGLAQLMRGRTTF
                     VIAHRLSTIRRADQILVVEGGRIVERGTHATLYAQAGRYRDLYDKQHGLEANLFLATG
                     EGEIPESAEANGPSTKNSDPDMLRLLRDRPA"
     gene            13553..14545
                     /locus_tag="VG272_09540"
     CDS             13553..14545
                     /locus_tag="VG272_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13963.1"
                     /translation="MSKLRLSMACWNYDRTRALFEERVPIDGIDLTYINIPVEETFFR
                     MIRHQEFDIAEMSLSSYTLSMFRDPKPFVAIPVFPSRYFRHSCIFINRNSGIREPKDL
                     IGKKVGNPEYQLTACVWIRGILSDEYNVPVSSVTYFRGGEETPGRTEKQTVSLPREIR
                     LEHISADKTLSKMLRDGEIDAIYSPRTPSCVAQGAESVGLLFEDYEAVEREYYKRTKI
                     FPIMHTVVIRREVYEKHPWIAQSLYKAFVLAQREVYEDLYETAALKFMLPWLIRHAED
                     TRALMGKDFWPYGVEPNLHGLSTFLRYAHEQGLAKRLLAPHELFVPESLESFKI"
     gene            14630..16153
                     /locus_tag="VG272_09545"
     CDS             14630..16153
                     /locus_tag="VG272_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13964.1"
                     /translation="MAESGQASALNWWRLERGDIDATVAQVGFNVAQMIIPAFLLVPV
                     GVAMGFSVGHLLPGYALGFFVGSMGLTMLGVGLAKREGRTNVTAHVYINNVPGIIAYT
                     LSIMLPVYLQTHDQELAWRLGAAAVVWTGIIKLVAAPLAGTIRQFIPKPAMMTVFGAA
                     MYTYLALVLLQRIFDQALVGIIALAIVATTVLANVPITKWRIPPFLVAWLAPLAIGLS
                     IGYVHPAWHGFSLQLPFARTPGLLQAMGLAIPYLSVIAPMSIYQVLQDIASVEGANSA
                     GDNYDARLVVACDGLGTLLCGLGGSIITPVVAALHPPYKMMGARIGFCFWTGIIFLAI
                     FMSGISLFMSQLFPWAILAAMIAYVAIGVGRATLHHVEHKYINAVLLGLVLPAGAVVG
                     SAMSSALPALKLSAASPEVQAALNRSIYWSSIQGLGNGFLFLVLVVSALITEMIDRNF
                     GRAALWCLIASVFSWLGLMHSPVLHWGAQPMYAAGWMAAAFITFSARWWRGDLPAAA"
     gene            16495..17622
                     /locus_tag="VG272_09550"
     CDS             16495..17622
                     /locus_tag="VG272_09550"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HWF13965.1"
                     /translation="MQKAATASTPGEFRRHLFYETSHSPSLAELVGYENAKDIIDFCF
                     IANPYYPTTAMLRDMQDNFPNLIKSYPPSNPASSQEHLAAVLNVNPTHLIIGNGATEL
                     IVLINTTLISRIAVPVPTFGEYIEKLKDQRDAELYILNPDERYTLHLPEYLEWVRKRG
                     LKSLLIINPGNPTGQLFCLEEMIEFLDQAKDMELVIVDESFIDFAGEKVPSLLHVAGR
                     FSNLLLVRSMSKHCGVPGLRLGYCYSENLYILNHMRQFIPTWNVNTLAQYFLSLLPTT
                     DADYHIGRKRLIGDVRWLYDSLKSISSLAVYPTGANFVLFKIESGMTATDLQGVLLND
                     HKLYVRDCSNKVGMDPYHIRVASQGHEKDAKLVTALQTILR"
     gene            17619..18368
                     /locus_tag="VG272_09555"
     CDS             17619..18368
                     /locus_tag="VG272_09555"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HWF13966.1"
                     /translation="MKLPAVLVVTRRTIRKNKYIDYVAEAHLRQLVHLKTLPLMVPVV
                     EGSRICLPDYLSEMCGLLIVEGEDIEPKHFKHTHENYTHLEKTHPLKDEIEIELIEHA
                     LRKQIPILGICRGSQLLNVVCGGTLCGDVRKEKKSKLKHINFEHYDSYRHPISIVAGT
                     PLQKWYGRKSLRVTSYHHQGVRDLAPRFQPMAFAKDGLIEGFYDPEKEFVVGLQFHPE
                     RMPEEPAGNWHIWQAFGAAIHSVGKKKKSRA"
     gene            complement(18582..>18774)
                     /locus_tag="VG272_09560"
     CDS             complement(18582..>18774)
                     /locus_tag="VG272_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13967.1"
                     /translation="ATSIGHQGLKPLHFKALNVAAEAATYKNFLSDRLALLTSRFRCR
                     SAVARLFRGGGLDDDLLKA"
BASE COUNT         4121 a         5478 c         5033 g         4142 t
ORIGIN      
        1 aggacaaacg cgcgcgcgag atgattggga taatcaagca gcggaggaaa cgcgacgacc
       61 caaatgggcg cgagaagcat cgcagccagg atcagcagga aggtaacgcg aaatattttg
      121 ttggcggaaa ttttttgcac gggccgcgtg ggcgctgatt ccatttctga agtgtaccgg
      181 gaatttctac cgcgcggagc atggttttca gtggcctttc aagtagcgca ggcttccgcc
      241 ggtgaggggt tggtgccggc aaggacgaaa ccccggaggt ttgaaatctt gcaagcacaa
      301 aattccgcag gctgaagacc gcgctactaa aggccatgcc agagcagagg cacaaggccc
      361 agcgcggtca gcgcgagcat caagcctaaa ctttggcggc ctgttttact gtccgctaca
      421 aatagcgcga taaacgcttt cgcaaaatta ttgctggccg cggcgacgac gatgccgcta
      481 atcgcaagcc ccatggttgt aacttttccc gcggtttgcg taaggcccat gatgaacggg
      541 tccaccggcg cgagtcctga aatcacagcc atgccataaa ttcccgcgct ccccaggcgg
      601 gacatcgcga aattcgtggc caccaacacg accacgaaga caaggcccag caggagtgcg
      661 gaggtcagct ccaacgggtt tttggtcagg tacgcgggct tcgctcccgt ttcttcctgg
      721 tcgtcggaca aattcgacca cgcccagccg ccggccacgc caatcgcgcc aagaatcaga
      781 aacggaagcg agaggtgctt catcagctcc agattaaaaa taccgatcag cgcgaggaag
      841 cgaatgtaca tcacgccgga tgcgatcagg atggcgcccg cgtaagtgtg cggcgatccc
      901 gcagtcttgg cttgcttgcc catcactacg gtggtgaagg tggaggaata cagcccgccg
      961 aggagcgccg cgagccgcac gccacctttt ccttttgaca atttctccag aaggtagctg
     1021 ccataggaaa ctccgctaat ggctacaaca atcagccacg agcggaaggg attgatgcca
     1081 aacggaccgt attcctggtt cggcaagatc ggcaaaatga caaacgtcag cagcaaaaat
     1141 ttggtgaagg tgagaatttc gatggcggga atttccgtgg acaggccttc aagatattct
     1201 ttcaattcca gcagcagcat gctgatcacg gcgatggtcg tggcaatcca aaactgatcg
     1261 cgcgagacga gcgcgccgat aacgtacgtc accaacccgg aaatttccgt cgtcactccg
     1321 gcttcgttgt acgtttccag tttgtgcttg taagagagcc agaggaacgc gccaatgacg
     1381 ccgaacccaa aggtgagcgg caaaagttgg gcgcccgaaa gcagcgccat cgcatagccg
     1441 atcaacccga tcagcggata cgtgcgcacg ccaccgaatc cgtagtgatg gacctcggcg
     1501 cgccgctctt ctcgctcgag cccgatcagg aagcacagga agagcgtcaa gagaatcttg
     1561 aacccttcca gcggcatgag ttgtaacagt ttatcggtca cgaatcgttt gatagcggcc
     1621 cctgtgcccc cgcgagacta tcgggaatca gaaggaaact caatggcgga attgttaatg
     1681 tattcgtttg gagtagaaag aattttgatg gatgatttca aggttcgcta atctgcaata
     1741 cggaaaaact tccgccagac gatatcttct ccgagagata catccacacg gtaaattcca
     1801 acgggcaagg ttgctaaagg aatttcccac gtgctggagc tgagcgctcc tgaatgcaaa
     1861 cttactttca ctggattgga ttgcccaagg gcatgattat cgacgtcata aaccgccatt
     1921 gccgcggttc ccttaaaaga aatgttttca ttccagttca catagaccca aattttttgg
     1981 tccttgtgag agaactgttg acttgggttg gacggggtcg gcacacccct ccccttttcc
     2041 aatgtcagcg ccaattctgc gattattatt ttttgctcgg aattgatgag cggcaccatt
     2101 tgccctttgc gtgtcagttc ctccagattt gtttgatcct ggctggatga ggcagggatc
     2161 agattgatcg gaacggcaag tccatcgcga atataggaag ttccacccaa gccatttcct
     2221 gccatagtca gcatatagga tcccagcaga tctgttcccg gggcgagact tccgccaacc
     2281 attccaacta catcgccgaa ctcattcgtc agaggtgatc caattgcccc gcgactcggg
     2341 ctttcggcga tattcatgcg ctcgccagct cgcggaaacg tattctggcc gacaattgtt
     2401 ccatcagcga tgacccttcc ggaaccggaa gaagtttgca gaaaataaca aatagaaccc
     2461 acgctccatg attttggcgc agcgcgagtc aggccgttca ccttcgcccc gctgatagcc
     2521 aagatagccc aatctttcca acgatcccat gaccggaccg cagtcacttc ctgcgctgaa
     2581 ccatccgggg aaatcactct cagcttcgtc gctccatcaa cattttgaaa agcagtaatg
     2641 agctggccat ccgccaggta aaaacccgac cctcgtgcaa ctgctttccc gtgggtatct
     2701 attttgtcga caaaaacgct gacatcggcc agccgtttgt aaagatcacc ggtcgaaaga
     2761 ggttggcgag gcggggcggg tgcaacgggc gttaccgcgc ctgggcctgc gatcagttgg
     2821 aaggaaaggt ctcctgcaga ctcgccatcg atatgagctt ccagggtcca caagccggga
     2881 gtttccgagc cgctgagcag catgctccaa tagcctgaga actgtttccc aggagcaacg
     2941 tactggaaat ctgaaatcaa cgtcaccttg ctatctgcat ttttccaaat tccctcgaat
     3001 ttgtgcagac cggcaggacc ttcccactca aaatacacga ttatcttatg gtcttgccca
     3061 atgtagaaca cgcttctggg atcatccaga atgagacgcc cattcgattc ttttcctgac
     3121 gacccagcca tcgcgcggag gactttgaaa gttggcgccc ccactgggcc gccgccgctt
     3181 tgcgcaatcg ctctgccagc aaagagcaag caatagacca gcgcggctat agtcgcaaat
     3241 aagaacgtcg aggaacgcgg ccttactttt atggtaagta agctcatggt accgtcgcga
     3301 gattctagca gaatctctgg tagttctctt cttacctttt ttgttggcag ttcttatcag
     3361 aatataaagt aggggtcggt cccatggcat attgctaaat tgaaacttgg aaggcctgaa
     3421 ttccgcaggc tgaagcctac gctccataaa acaactagct gagggtcgtg ttgcccatta
     3481 aatatttgtc gacggaggca gccgccttgc ggccttccgc gatggcccac acgacgaggg
     3541 actggccccg tcgcatgtcg ccggctgcga aaacgccggg aacggagctc atgtagtccg
     3601 cgccggcggc aatgttgccg cgctggtcga gattcacgcc aagctgctcg atcatgccgt
     3661 ttctcacggg gcccaggaag ccgagcgcga gcaacacgag gtcgacctcc atcacgagtt
     3721 cggtgccggg gcgtggctcg aatttcggcg gcgggccgac gcgcatagcg tgcagctgct
     3781 ttacgttgcc ctggtcgtcg cctgtgaatt tcgccgtgtt cagcgcccag tcgcgattgc
     3841 cgccttcttc gtgcgcgctt tcggtgcgca gttgcagtgg ccacaacggc cacggtgtgc
     3901 tcggagcgcg ctcggcggga ggcataggca tgatctcaaa ctggtgaatg gattttgcgc
     3961 cgtggcgatg gcaggtgccg aggcagtccg caccggtgtc gccgccgccg atgatcacca
     4021 cgcgcttgcc tgtggcgaga atctgctccg gaatcgtatc gcccgcgatc actttatttt
     4081 gctgcggcag aaattgcatc gcgaaataaa tgcccttgag ttcgcggccc ggcacgttca
     4141 gatcgcgagg atgttccgcg ccgccgctta aaacgagtgc gtcatattcc ttgcgcaaat
     4201 cctgcgcggg aatgctcgtg cccacgtgcg cgttcagctg gaattgcacg ccctctgcca
     4261 tcatctgctc gacgcggcgg tcgacgacgc gcttctccaa tttaaaatcc ggaatgccgt
     4321 aacgcagcag gccgccgatg cggtcggctt tctcgaaaac agtgacgtca tgccccgcgc
     4381 gattcagttg ttgcgcgacg gcaagtcccg acggaccgga accaaccacg gcaattcttt
     4441 ttccagtgcg cgtctcgggc ggctcgggca gaatccagcc ctcttcccac gcgcgatcga
     4501 caatcgtttt ctcgatcaac ttaatggtaa ctggcggctc gttgatgccc agcacgcaag
     4561 ccgcttcgca cggcgcggga caaatgcgcc cggtaaattc cggaaaatta ttggtcgcgt
     4621 ggagaacgcg aatcgcgtcc ttccagcggt ccttgtaaac cagatcgttc cagtccggaa
     4681 tgatattgtt gaccgggcag cccgtatggc agaacggaat gccgcaatcc atgcagcgcg
     4741 ccccttgtgt gcgaaccttt tcttctgaga acggctggta gacttcaaaa taatcgtgaa
     4801 tgcgctccgc cacaggacgt cgcgccggtg tttcccgctg gtattccttg aagccggtga
     4861 ccttacccac ggagcgcctg cccctgtgcc tgcgcgcttc ctgcctgcgc cgctacgacg
     4921 gtcgcgggaa tgcgcggcac gcccagcaca cgtttgtact cgtgcggaaa aacttttacg
     4981 aatttagaca gcgcggaatc ccagtgtttc aatatccgtt gtccctgtgg actgtcggtg
     5041 agctccgcat ggcgcgtgac caggcgatgc aaaacttcga tgtccttggt gtcggtcaca
     5101 ggttcgagat cgacgtccgc gcgattgcag ctggcttcgg caaattcgcc ggactggtcc
     5161 agaacatacg cgataccgcc cgtcatgccc gctgcgaaat tgcgtcccgt ttttccgagc
     5221 actacgacca ggccgcgcgt catgtattcg caaccgtgat cgccaactcc ctcgacgacg
     5281 gctgtagcgc ccgaattgcg caccgcgaac cgctccccgg ccatgccgtt gaaaaatgct
     5341 tcgccgctcg tcgcgccgta taggcatacg ttaccaacta gtatattttc ttcgggtacg
     5401 aattgcgaat tgcgattgcg cggcggataa atcacgatgc gcccgccgga aagccccttg
     5461 cccacgtaat cgttggcttc gccttccagc gtgagcgaaa cgccggcaga gaggaacgcg
     5521 ccaaagctct gccctgcgca gccgtggaat tggatgcgca ccgtttcatc cggcaagcca
     5581 gctgagccgt gcttgcgcgc aatcctgccg ctgagcatcg caccgaccgt gcggtgaacg
     5641 ttgcgaatcg gcaaattgaa agacacgggc gtgagccgct caatcgcatc cgttgcctgc
     5701 tcgatcattt ttccgtccag cgcatcgctc aggccgtggt cctgcgcctg cgtgcagcgg
     5761 cggcccacgt ggctcggcat cggcggattg tagagcactg aagatagatc gatggacttg
     5821 gccttccagt ggtccacatc cttgcgctgg acgaggcaat ccacgcggcc aatcatttcg
     5881 tccattttgc ggaagccgag ttcggccatg atcaggcgca aatcttcggc cacatagaac
     5941 aggaaattaa tgacgtgctc gggttgtccc gaaaatttct tacgaagctc aggatcctgc
     6001 gtggcaacgc ccacagggca ggtgttcaag tggcatttgc gcatcatgat acagcccatc
     6061 gcgatcagcg gcgtcgtagc aaagccaaat tcctccgcac cgagcaaagc gcccacggcg
     6121 acatcgcgcc cggtttggag cttgccgtcc acctgcaggc gcacgcggct gcgcaaatcg
     6181 ttgagcacca gaacttgctg cgtctcggac aagccgagtt cccagggaat gcccgcgtgg
     6241 cgaatcgaac tcaccggcga agcgcccgtg ccgccatcat agccgctgat gaggatcaca
     6301 tcggcatgcg ctttggcgac gcccgcagca actgttccga cgccgacttc ggccacgagc
     6361 ttcacggaaa tgcgcgcctg tggattcaca tttttcagat cgaagatgag ctgcgccaga
     6421 tcttcgatgg aataaatatc gtggtgcgga ggcggagaaa taagtccaac gccgggaatc
     6481 gagtggcgca cgcgggcgat cacgtcgtcc actttgtgcc cgggcaactg gccgccttcg
     6541 cccggtttcg cgccttgcgc gatcttgatc tgcaaatcgt cggcgttaat taaataattt
     6601 gtggtgacgc cgaaacgcgc tgaagccacc tgcttgatcg cgctgcggcg tagatcgcca
     6661 ttcgcgccgg gagtgaagcg tgcttcgtcc tcgccaccct ctccggtatt cgatttgccg
     6721 ccaatgcggt tcatcgccac ggccagcgtt tcgtgcgctt ccttgctgat cgagccgaag
     6781 gacattgcgc cggttgcaaa gcgcttcacg atttccgaag cgggctcgac ctggtcgagc
     6841 gggattgccg ggccgttcgg tttgaagtcg aacatcccgc ggagcatctg taaatcgcgg
     6901 ttctgctggt tgacgatgtc agcaaattcg cggaagcttt caaacttttc ctgccgcacg
     6961 gcgtgctgta acttgctaac cgtggtcgga ttcaagaggt gccgctcgcc gcgcacgcgg
     7021 tattgatact cgccaccgac acgcagttcg gtatcggaca agtgcggcgg ctgcatcgcg
     7081 aagtcatgct tcatgatcgc ttcgcgcgcc agcacttcga tcccgacgcc ctgaatgcgc
     7141 gacgatgtgc cagtgaaata tttttccaca agcgacttat ccaagccaat cgactcgaaa
     7201 atctgcgcgc cttgatagct ttgcagcgtg gagattccca tctttgagaa cgttttcagc
     7261 agccccttgt cgatggactt catgtagctt ttgaaaactt tgtcccacgt ggtgtccgcc
     7321 ggaaacgcgc cggacctgtg caaatcttcc agtgtttcga tcgcgagata cggattcacc
     7381 gcgctcgcgc cgtagccgag cagcagtgca aaatgcatca cttcgcgcgg ctcgccggac
     7441 tcgacgatca gcgcaacctg atttcgcgtt ccttcgcgaa tcaaatgatt gtgcacggcc
     7501 gacatcgcca gaagcgacgg gattggcgca tactcatgat cgacgccacg atcagtgaga
     7561 atcagcaatg tgtatccgga cttgatggcg agcgacgcgc gccggcaaag attttcgatg
     7621 gactcttcca gttgctgctc tccgccgctc acgcggaata gcatgggcag gctcgtcgcc
     7681 aggaaactgc cccagctgac gcgccgcaat ttttccagcc gccaattgga aattaccgga
     7741 tgccgcagtt tcagcgtgtg gcaattcttc ggtgtctctt cgagaatgtt gccttcgctt
     7801 ccgatgtagg aattgagtga catcacgagg ccctcgcgaa tcgaatcgat tgccgggttc
     7861 gtgacctgtg cgaaagtctg cttgaaataa ttgaacagca tttgcggctt gtccgacaaa
     7921 caagcgagcg gcgtgtcaat gcccatcgag ccgacaggct cttcgccgtt cagcgcggaa
     7981 ggcagtaaaa ttgttttcag gtcttcgtcc gtgtagccga acgcgcgctg ccgcatgcgg
     8041 atcgtgtcat ggtccgtcgg ctgcacgtgc gtgggactgg gcagatggtc gagcgtgatc
     8101 tgattttcct tgagccactg cgcgtaaggc cgccgcgccg ccagatgctt tttcacttct
     8161 tcgtccgtga tcagccgctt ctgttcggtg tccaccagga aaatgcggcc gggcgcaagc
     8221 ctgcctttca agcgcacatc ttccggtttg atcggcagca ctcccgcttc ggaggcgagc
     8281 acgacgaggc cgtcgttcgt cacgaggaac cggccagggc gcagaccatt gcggtcgagc
     8341 ttcgcgccaa tcacgcgtcc atcggtgaac gcgactgccg cggggccgtc ccacggttcc
     8401 atgagcgaag cgtgatattc ataaaatgcc ttgatgtcct cgggcatcgt gtcgtcatga
     8461 tcccaggctt cgggaatcag catggacatc acgtgaggaa gcgagcgccc ggaaagagtg
     8521 agcagctcga cggcattgtc cagcatggcc gaatcgctgc cgcccgccgt gatgaccggc
     8581 aaaagtttct tgatgtcatc gccaaacagc ggagactgca gcacggattg ccgcgcatac
     8641 atccagttca tgttgccgcg cagcgtgttg atttcgccat tgtggcagat gtagcggtag
     8701 ggatgcgcca gcttccagct cggaaacgtg ttggtcgaaa accgctggtg cacaaggcac
     8761 agcgcgctcg tcacatcagg gtccagcaat tctttgtaaa aatccgtgat ctgcggcgcg
     8821 agcagcaggc ctttgtagat gatcgtgcgc gaggaaagag acggaatgta gaaaaattcc
     8881 ttttccttca gatctgtggc caaaatttcg gcctcagttc gcttgcggat cacatacagt
     8941 ttgcgctcca gcgaatcctg atccatgccc ggggcgctgc gcacaaaaat ctgctcgatg
     9001 tatggctgtg aagcgcgtgc cagacgccca atcgtgttgc cattgatcgg cgtgtcgcgc
     9061 catcccagaa cggccagccc ctcttcgcga acaatctttt cgatgatgcc ttcgcaaatc
     9121 agccggtcgt gcgtatccac tggaagaaaa accatgccaa cgccgtactc gccgggacct
     9181 ggaacggaga agcccatgtt cacagcttcg cgctcgaaaa atgcgtgagg aatctggatc
     9241 agaatacctg cgccgtcgcc cgtttgcgga tcgcatccgc aagcgccgcg atgggccaga
     9301 ttaatcagga tctgcacgcc cttcgtgaca atgtcatgcg acttgcgtcc ctcaatgctg
     9361 gcgacaaatc cgatgccgca ggcgtcatgc tcgtgctgag ggttatacaa cccctgagcc
     9421 ggtggtacgc cacgaaatgg gtatagttca ctcatgtaag caggaattaa cgatccaggt
     9481 gttgctgaat tttcacggcg cagcgtgcgc cttggattct gcgaacttaa tcctaattta
     9541 ctcgatcccc tgaaacgcca cagcctcctt ggcctgacca tttgatacta atctgtttat
     9601 ttcgatgccg cctaggttcc aatttccgaa aagacatccg gggaagcggg ggcctaatag
     9661 acttcagcgc gcccctgaac tgctttcggg aaagtgatta tcttaccatt cttaggctcg
     9721 gttgtccgca ggccattagg catctcgatt tgttatccgt agcattattt ttttggatta
     9781 gctgtaagca atcaccctga ttctgctata atcccgctct caaccatgga tttcgaccag
     9841 ctcactactt ttattgaaat tgccaaactt ggcagctttt cccgcgccgg acaaaagctg
     9901 ttccgttcgc agcccgctgt cagcgcccaa gtgcgccagc tcgaacagga atacggcgaa
     9961 aaactattcg atcgcggacg caagtccgtg cgcctgacct cggcgggcga aacccttttt
    10021 gaatatgccg tgcgcatggc caacttgcgc gccgaatcgc agcgcgccgt ggccgacaaa
    10081 gctaccacgc cgcgaggcgt cctcgcgctc ggcgcgaacg aagccacgtg cttgtacgtt
    10141 ctgccggata ttttcgcgga gtatcatcgc ctctaccctt ccgtgcagat cagcatctat
    10201 cgaaatttca gtcacaaggt tttgcagaag gtggaggacg gatcgatcga cgtgggcatc
    10261 gtcacgctgc ccgcgaaatc cgccagcctg aagtttcacc caatcttccg cgatcgcgtg
    10321 atgcttatgg tcagcccgcg cagcccgctc gccaaactga aaagcgtgcg catgaaagat
    10381 atcgccgacc agccgctaat tttcccgcgc acgggttaca ccaggcagtt gcttgacaaa
    10441 catttccggc cttacaaacc gcagctccag atcacgatgg aattgaccag cgttggcatg
    10501 atcaagtgct tcgtggccgc caatctcggc gtgtccttaa tcagctcaag cttcgcgcgt
    10561 aacgaagtcc gcgccggcga agtcaagctg attcccattc aagacgtcga gctatggcgc
    10621 gagttgggaa tcgtctaccg ccgcgaccgc actttgccgc gctcctcttc ggcctttatt
    10681 caactagtca ggcagcgagc cgcggaaaat cccgacctag aaatcccgcc gcgcaaagcc
    10741 gcacgcgcca gcagcgaagc cgccccgctt ccttaagccc tatctgcaac tcaaaatctt
    10801 ttgattggtt taagtaccgt aggcattaca ggctgcggaa aaggtatttt atactgtcat
    10861 tccgagcgca gcgaggaatc tctcttcgat ttaacgtgcg aaacgaaaga aattcctcgc
    10921 tgcgctcgga atgacagaat ccagttcgac gtattgcggc gctgaaagcg cgggagcatg
    10981 ccagcccagg gcaacgccct gggtacacgt ccccgcacca acatcttgcg ccctgaaggg
    11041 cgcaaagtcc ctccgccctt tcacggcgtt ctaccatttg gtgggcttgt tatccggggc
    11101 tttgccccgg gctggtatcc ttcgcgccgt tggcgcgcaa atcccggagg atttcaattg
    11161 gagatcttca aaatagaaaa cttctgcacg gtattgatga gtgtgctttg tccgcggagt
    11221 ttccctctaa tttcaattta cgccggccga tcacggagca aacgcaacat atccgggtcg
    11281 gaatttttgg tggaaggacc attcgcttct gcggactccg gaatttctcc ctcgccggtg
    11341 gccaaaaata aattggcttc gaggccatgc tgcttgtcgt ataggtcgcg ataccgccca
    11401 gcctgcgcat aaagcgtggc gtgcgttccg cgctccacga ttctcccgcc ttcgaccacg
    11461 agaatctgat cggcgcggcg aatggtggac aggcggtgcg caataacgaa cgttgttctc
    11521 ccacgcatga gttgcgcgag cccttcctga atgagcattt cggattcgga gtcgaggctc
    11581 gacgtcgctt cgtccagaat caggatgcgc ggatcggcca gaatcgcgcg agcaatcgaa
    11641 acgcgctggc gctgcccgcc ggaaagtttc acgccgcgct cgccgataat cgtgtcgtag
    11701 gtttccggaa aacgttccgc gaattcgtcc acgcgcgcaa tacggcacgc cgccagaatt
    11761 tcttcttccg tcgcatacgg ccgtgaaaac gctacgttct cgcgaatcgt accatcgaac
    11821 aaaaacgatt cctgcaatac gacgcccagc cgcgtacggt acgaatcgag ccgcaccgtc
    11881 gaaaggtcca cgccatccac cagcactcgc ccgctcagcg gaacgtgaaa tgccgccaca
    11941 agaccgatag tcgtcgattt tccagagccc gaagaaccga cgagcgccgt gacagtgccc
    12001 ggcttggaaa ggaagcttac gtcctgcagc accagcttgc ctggctcgta agcaaagttc
    12061 acgtcgtcga acgccagctc gccaacgata tccggcagcg taaccgtgcg tcgcgggtct
    12121 tcatcctcag gccgctcgtc gagcacttct ttcgtgcgct ccagccccgc aacggcttcg
    12181 gtcaattgcg tgccgatgcc caccacttga aacaccggcg cgatcatgaa ggcgagaaac
    12241 gccgtgtaac tcatgaattg tccaaccgtg attttaccct gcagaacctg gtgcgaaccc
    12301 acgagcatca caagggcacc gacgacaccg ccgagagcgg tcgtcgaaaa actcatcatg
    12361 gacatcgtcg tgagcgattt aatgacgttg tcgagcaatc gctgcacgcc tttcgtgaac
    12421 acatcggctt cgcgatcctc tgcatggtac cccttcacca cgcgcacgcc ggaaagcgat
    12481 tccgtcaaac ggccgctaac ctcggcattt attttcgatc gctcgcgaaa gatcgggcgc
    12541 aaaatcccaa atgcttttct caggccgaac atgctcacga gcagcagtcc tgcagcgagc
    12601 cccgtcatca ccggactgat acgcagcaac atgtagagag caatgaacgc cgtcagcaac
    12661 ccccccagaa attccacaag tccggtgccg atcaagttcc gcacgccctc cacgtcgctc
    12721 atgatgcgcg aaacgagcgc gcccgtttta ttctcatcgt aataagcgac cggcaagcgg
    12781 cctacatgct cctgaacttt tctccgcagc tcggtgatca tgcgctgcgc ggctttcgat
    12841 aaagtttgcg tgagcgtgta agacgtcacg ccttgcagga tcgtcgctag gagcactcca
    12901 ccaacgagcg gcacgagcat ccgcgtctga tggtggatca ttacgttatc cactaaatat
    12961 tttgtggtga atggaagcgc gagaccggac acacgattaa tggccatgag caccatgcca
    13021 atgccgagca agcctcttcg tggccgcatc aacgcccaga tttgaggcag ttgatccaaa
    13081 atgcgaacag gcttcttctt gctcgcgtcg gacggatctc cacggccacg cctcgcgcta
    13141 tgtccgcgct ccacgcgcat ggccatgcca tgctgcgatc ccaatcctcc gcccggcatt
    13201 ctcataggtt attcatctta gtggattgcc actggcgccg ccactgactc cgcagcgcgt
    13261 ctgcggttgt cgcgcggaat gccttgaatc ccggacagac gcattacgat gatcagatgc
    13321 ggtaatcatt ggtcttgatg cagaaaaatc tcagcatgaa agaatgattc attacacctg
    13381 tgcctgttta agagaaatat taaaaataaa attacaccgg ccgaaattca tcggtaagat
    13441 gtgcaagctg cggtttggcc accggactcc tgcgcgattt gccatttgcg agtatagtcg
    13501 tgcgaatccg gaagtagccg gaagcgggac gttctccacg gagacataca acatgtccaa
    13561 acttcgccta tcgatggcct gctggaatta tgaccgcacc cgcgcgctgt ttgaagagcg
    13621 cgtccccatc gacggaatcg acctgacgta catcaacatt cccgttgagg aaactttttt
    13681 ccggatgatt cgccatcagg aatttgacat tgcggaaatg tcgctctcct cctacacgct
    13741 ttccatgttc cgcgatccaa aaccttttgt ggccattccg gttttccctt cgcgatattt
    13801 ccgtcactcc tgcatcttca tcaatcgcaa cagcgggatt cgcgagccca aggatttgat
    13861 cggcaaaaaa gtcggcaacc ccgaatacca gctcacggcc tgcgtgtgga ttcgcggaat
    13921 cctgagcgat gaatacaacg tgccggtttc gagcgtcacc tattttcgag gcggagaaga
    13981 aactcccggc cgcaccgaaa aacaaactgt ttcgctgccg cgggaaattc gcctggaaca
    14041 tatttccgcg gacaaaactc tttccaaaat gctgcgtgat ggagaaattg acgccatcta
    14101 ttctcccaga acgccttcct gcgtcgcgca aggtgccgaa agcgttggcc tcttgtttga
    14161 ggattacgaa gcagtggagc gcgaatatta caaacgaaca aaaattttcc cgattatgca
    14221 cacggtagtc attcgtcgcg aagtgtacga gaagcatccg tggatcgcgc agtctttata
    14281 caaggctttc gtgctggcgc agcgcgaagt gtacgaggac ctgtacgaaa cggcggccct
    14341 gaaatttatg ctgccatggc tcatccgcca cgcggaagac acgcgcgccc tgatgggaaa
    14401 agatttctgg ccctatggcg ttgagcccaa cctgcacggg ttgagcacgt ttcttcgcta
    14461 cgcgcatgag caggggcttg cgaaaagatt gttggcgccg cacgaactct tcgtgccgga
    14521 atcgctcgaa tcatttaaga tttgaaacct cgcattttag ttttcatttc cagaatttcc
    14581 ggccaggaat tgcgaagctg cgaagaactg ttcaagggag atcaccggaa tggcggaaag
    14641 cggacaggcg agcgcgctca actggtggag gctggagcgc ggagacattg acgctaccgt
    14701 ggcgcaggtc ggcttcaatg tcgcgcaaat gattatcccc gcatttcttc tcgtacctgt
    14761 cggcgtcgcc atgggcttca gcgttggaca cttgctgccc ggctacgcac tgggattttt
    14821 cgtcggctcg atgggcctca cgatgctcgg cgtcggcctc gcaaagcgcg aaggccgcac
    14881 gaacgttacg gctcacgtat acatcaacaa cgtgccggga attatcgcgt acacgctgtc
    14941 catcatgctt cctgtgtatc tgcaaacgca cgatcaggaa ctcgcgtgga ggctcggagc
    15001 tgcggctgtc gtttggaccg gaattattaa acttgtagcc gcgccactgg caggaaccat
    15061 tcgccaattt attcccaagc ccgcgatgat gaccgtgttc ggcgcggcca tgtacacgta
    15121 tctggcgctg gtgctgctgc aacgcatctt cgatcaagcg ctcgtcggca tcatcgcgtt
    15181 ggccattgtc gccacgaccg tgctggcgaa tgtgccgatc accaagtggc gcattcctcc
    15241 cttccttgtc gcctggctcg cccctctcgc gattggcttg tccatcggct acgttcatcc
    15301 cgcatggcac ggtttttcgc tacaacttcc cttcgctcga acaccgggcc tattgcaagc
    15361 gatgggcctc gcgattccct acctgtccgt gattgcgccc atgtccattt accaggtgct
    15421 gcaggacatt gcctcggtcg agggtgcgaa ttccgcgggc gacaattacg atgcgcgcct
    15481 cgtggtggcc tgcgacggtc tcggcacgct tttgtgcgga ttgggaggaa gcatcatcac
    15541 ccctgtcgtc gctgcgctgc atcctcccta caaaatgatg ggcgcgcgca tcggtttctg
    15601 tttctggaca ggcatcattt ttctggccat attcatgagc ggaatttccc tgttcatgtc
    15661 gcagcttttt ccctgggcaa tccttgcggc gatgatcgct tacgtggcga tcggagtcgg
    15721 tagggcgacc ctgcatcacg tggaacataa atacatcaat gccgtgctgc ttggcctggt
    15781 gttgcctgcg ggagcggtcg ttggttcggc gatgagttcc gcgcttccag ccttgaaatt
    15841 gtcagccgct agtcccgaag tgcaggccgc gctcaatcgt tccatctact ggtcctcgat
    15901 tcaaggactc ggcaacgggt tcctatttct cgtgctggtg gtctccgctc tcatcactga
    15961 aatgattgac cgtaattttg ggcgcgccgc gctgtggtgc ctgatcgcat ccgttttctc
    16021 atggctaggc ctgatgcact cgcctgtcct tcactgggga gcgcagccga tgtacgcggc
    16081 aggatggatg gccgcagcct tcattacatt ttcagcgcgc tggtggcgcg gcgacctgcc
    16141 ggcagccgcc tagattccca gatttttgca aggcacgcaa aaatctgccc gcccaagatt
    16201 gggcggcccc taggtttcgt agcggcctgt ctgcaccgcg tacttcgggg cgaacagggc
    16261 acgcggtcta acaccccagg cacgctatcc gtcgatctca atttcacccg cagtctgaat
    16321 cgcactcgca caaaaccgag cccgtccaaa aaacggccgg cctctacgaa tgcaactgcg
    16381 gcttccgtga cgccgtcccc acaagtcaac tacgccagct actccatttt gctcagcgtc
    16441 acacaaaaag aaccaaccct tgtaagcctt cccttctaaa tgctacaaaa gaccatgcaa
    16501 aaggcggcca cagcttccac gcctggcgag tttcggcggc atcttttcta cgaaacttcg
    16561 cattctcctt cacttgccga actggttggc tacgaaaacg ccaaggatat tatcgatttc
    16621 tgtttcatcg ccaatccgta ttacccgacc acggcaatgc tccgcgatat gcaggataat
    16681 ttcccgaacc tgatcaagtc ttaccccccg agcaatccag ccagcagcca ggaacacctt
    16741 gccgctgtgc tgaatgtgaa tccgacgcac ctgatcatcg gcaatggcgc gacggaactc
    16801 atcgtcctta taaacacgac gctgatcagc cgcattgccg tgcccgtccc aactttcggc
    16861 gaatacatcg aaaaactgaa agatcagcgc gacgcggaac tctacatcct gaatccggat
    16921 gaacggtaca cgctgcattt gccggaatac ctcgaatggg tgcgcaagcg cggtttgaaa
    16981 tctttgctga tcatcaatcc gggaaatccc acggggcagc ttttttgtct cgaagaaatg
    17041 atcgagtttc tggatcaggc caaagacatg gaactggtga tcgtcgatga atcatttatc
    17101 gactttgccg gagaaaaagt tccgagcctg ctgcacgtcg ccggacgatt ttccaatctt
    17161 ttgctggttc gcagcatgag caagcactgc ggcgtccccg gcctgcgcct gggttactgc
    17221 tacagcgaaa atctgtacat cctgaatcac atgcgccagt tcattcccac ctggaatgta
    17281 aacacgctcg cgcaatattt tttatctttg ctaccgacca cggatgccga ctaccacata
    17341 ggccgcaagc gcctgatcgg cgatgtgcgt tggctgtacg actccctgaa atccatttcc
    17401 agtcttgccg tgtatcccac gggtgcgaat ttcgttctct tcaaaattga gagcggcatg
    17461 accgcaacgg atttgcaggg cgttttgttg aatgaccaca agctctatgt gcgcgactgc
    17521 tcgaataaag ttggcatgga cccataccat atccgcgtcg cttcgcaggg ccacgaaaaa
    17581 gacgctaagc tggtcaccgc gctgcaaacg attcttcgat gaaacttccc gcggttttag
    17641 ttgtcacgcg cagaaccatc cgtaaaaata aatatatcga ttacgtggcc gaggcgcacc
    17701 tccggcaact ggttcatctg aaaacgctcc ctctcatggt tcccgtcgtg gaaggttcgc
    17761 gtatctgcct tccggattat ttatcggaaa tgtgcggcct gctcatcgtg gagggcgagg
    17821 acatcgagcc aaaacatttc aagcacacgc atgaaaatta cacgcatctt gaaaaaacgc
    17881 atccgctgaa agacgaaatt gaaatcgagc tgatcgagca cgccttgcgc aaacagattc
    17941 ccatactcgg gatttgccgc ggctcgcaac ttcttaacgt cgtgtgcggc ggaacactat
    18001 gcggcgatgt gcggaaagaa aaaaaatcca agctgaagca tattaatttt gagcactacg
    18061 atagttacag gcatccgatt tccatcgtcg ccggaactcc cctgcaaaaa tggtatggaa
    18121 ggaaatcctt acgcgtgacc agctatcatc accagggcgt gcgcgatctc gctccgcgct
    18181 ttcagcccat ggcctttgca aaagatggat taatcgaagg tttttacgac ccggaaaaag
    18241 aatttgtggt cggcctgcaa tttcatccgg aacgcatgcc cgaggaaccc gcgggaaact
    18301 ggcacatctg gcaagcgttc ggcgccgcca tccacagcgt gggaaagaaa aagaaatcgc
    18361 gcgcttaaaa taagccgtta aaaatatcgg gcggcatttc tagaaacgat ctggctctgt
    18421 tgacgtcctg ggtgcttcag aaattttcga ccgttttgtg tagcaggaat tgtagctcgc
    18481 ctcttcagag gcgagggttt agatgaaagt gtaaccaaca tttcctgttc agcgcgtgtg
    18541 ccggggtttc aagctgcgga ccaaccgccg gtacccttat ttcaggcttt gagtagatca
    18601 tcgtctaaac ccccgcctct gaagaggcgg gctacagcag atcggcagcg gaaccgggat
    18661 gtcaatagag ccaagcgatc tgataaaaaa tttttgtagg tcgcggcttc agccgcgaca
    18721 ttcagagcct tgaaatgcaa gggctttagc ccctgatggc cgatcgaggt tgcc
//
LOCUS       DASZMB010000209        18021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1021538, whole genome shotgun sequence.
ACCESSION   DASZMB010000209 DASZMB010000000
VERSION     DASZMB010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 18021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18021
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1021538"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..1069)
                     /locus_tag="VG272_09565"
     CDS             complement(215..1069)
                     /locus_tag="VG272_09565"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWF13968.1"
                     /translation="MDSTPPQTPREKLEAWLRYYDDLNLGAFYTDRRTMPKRASKKRA
                     PVKALASKATTAKHEVERPVMTQAPVKIAATLPALPIATVPSMFDIAERIEGDTLTRI
                     REDLGECTRCRLHTTRNKIVFGAGSAHAELVFVGEGPGRDEDMQGLPFVGRAGKLLTQ
                     MIEAMGLTRDQVYIANVVKCRPPENRKPEEDESATCSPFLYRQIDAIAPKAIVCLGLT
                     AAQSLLKTKDSISKIRGQWLDYRGTKLMVTYHPAYLLRNPPAKREVWDDLQKVMALLG
                     LKVKKVSK"
     gene            complement(1084..2331)
                     /gene="coaBC"
                     /locus_tag="VG272_09570"
     CDS             complement(1084..2331)
                     /gene="coaBC"
                     /locus_tag="VG272_09570"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HWF13969.1"
                     /translation="MRVALGVSGGVAAYKAAELVRKLQQDGIEVQVIMTDSAQEFITP
                     LTFAALTGQKVITEMFGAESEGGANVESAIEHIAVAQRIDLLVIAPATANTIAKMARG
                     IADDFLTTLYLATTAPVVVAPAMNVNMWEHAAAQENVEALRQRGVHIVAPEEGYLACG
                     MTGAGRLASVDAIASAVLARLGIAHDLQEETIVVTAGPTCEDLDPVRFITNRSSGKMG
                     YALATAAARRGANVILISGPTRLDPPQGVQFISVRTTEQMHRAVLENFQGATSLIMAA
                     AVADYRPVTPQTKKMKRGTVRLNLELEPTVDILSDVSRGKANRVIVGFAAETENVAAN
                     ARAKLESKDADLIVANDVTAEGAGFDHDTNVITLYFRDGSEKTFERMPKIDAAHRILD
                     HLRGLRPSSGNSLEQPAETQRTR"
     gene            complement(2347..2574)
                     /gene="rpoZ"
                     /locus_tag="VG272_09575"
     CDS             complement(2347..2574)
                     /gene="rpoZ"
                     /locus_tag="VG272_09575"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HWF13970.1"
                     /translation="MAPESQAPDSKFAFVVVAARRARQLMLGASPLLANPHSQKSTRV
                     AVEELRAGVLEYQLPDIPGDLLDKDGKRRKD"
     gene            complement(2582..3211)
                     /gene="gmk"
                     /locus_tag="VG272_09580"
     CDS             complement(2582..3211)
                     /gene="gmk"
                     /locus_tag="VG272_09580"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HWF13971.1"
                     /translation="MTLAHAQTPLVFIVSGPSGSGKSTLVQKMLEVPGTMFSVSCTTR
                     PPRATESAGKWYDFVSEDEFERRVQSGDFLEHARVFGKHQYGTPRRWLDEAQCKGLDL
                     VLEIDVQGAEQVKQKLPHAVAIFILPPAKEELERRLRARGQDSEEAIKRRLERAEQEI
                     RRYTAYDFLVVNDDMDRAGREIQAITIAARCLRSCLSQRAHNILESFGG"
     gene            complement(3208..4137)
                     /locus_tag="VG272_09585"
     CDS             complement(3208..4137)
                     /locus_tag="VG272_09585"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HWF13972.1"
                     /translation="MKATTATEGIYSMTGFAQARVEREGSAVRINLRSVNHRFLDLHL
                     RMPDGFEVFESRIRQAVRNRLRRGHVDINIYYEAGGTGAIEVNHEIAESYLRAVERLR
                     KEFSVKAEPDLVSLLRLPGVVAAPGTAGELQSEEKQERLGAQVEACLEQALNKLEAMR
                     RLEGQALLDEMQGMLGRIGTRTAEIETLTERVRPAIAHRLEIRLGELLKGVQVDPIRL
                     AQEASILAERSDVSEELARLRSHVEQFGKLLTGVGEAGKKLDFLLQEMQREANTLLSK
                     TPGVESEGLAITGLALEVKSDIEKLREQAQNVE"
     gene            complement(4134..5984)
                     /locus_tag="VG272_09590"
     CDS             complement(4134..5984)
                     /locus_tag="VG272_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF13973.1"
                     /translation="MNKREELGRLLRYVMPYKMRLAAGVVCLSVVGLAEGLIALMITP
                     IFDRVLNPQAPDSKLLLIKNPLTHQAIYLNSFLPSSIHSVGTLFAIAILTVFIGKGFA
                     EFLGNVLVQHAGIAGVTDLRNEVYSKVIRQPIGFFQHHQTGRLLSTVINDVERVRNTF
                     SEYLALGFRHIFTLFFLLAVLFATSWKMTLGSIILLPMVVVPVRTLGKCIRRSTEKIQ
                     AGIGELSQILEETVSGNRVVKAFGMEDFEIGKFRVSAERLLRENMRWIRAAVATSPLM
                     DLLGAVVIVLVLMYARVQIKTGAMTAGIFVTFIYALFKSYEPVKGLGTVYQQFELAFG
                     ATAKVFEYIGSQGEPAAEPGLPTLPPFSRSVDFDRVSFGYDTETPILREISLQARAGE
                     VIAIVGSSGAGKTTLVNLLPRFYPVTSGILRIDGMDVREVTLRSLREQMAIVTQETIL
                     FNDTVWNNICYGQPGLPKERVEAAAKAALAHEFIAELPQGYRTMLGDRGQRLSGGQRQ
                     RIAIARAILKDSPILILDEATSELDSESEMLVQRALSNLMMDRTAFVIAHRLSTIRRA
                     DKIIVLDDGKIAETGTHQELLARSGIYARLYEMQFADMDDAATPAPKADR"
     gene            complement(6252..7127)
                     /gene="rapZ"
                     /locus_tag="VG272_09595"
     CDS             complement(6252..7127)
                     /gene="rapZ"
                     /locus_tag="VG272_09595"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HWF13974.1"
                     /translation="MKRRSARDPRQLVILTGLSGSGKSTVLKTFEDLGFYCVDNLPVA
                     LIPTFSELHMRSGGEIQRAALLVDVREGDQLEKLPAILRQLRAEDPATLVFIEATEDA
                     LLRRFSETRRPHPLGHDRPVLEGLRRERVLMEPIRKLADVVIDTTKFNVHELRQFITR
                     SFQKTDRRPMMVSLVSFGYRHGLPADADLVFDVRFLPNPHFVPKLRKYSGRDPRVARY
                     IRSFPQTEEFLRRVEGLLIYLIPHYIREGKSYLTIAFGCTGGRHRSVMLAEAVRKTLQ
                     KKGYASKVVHRDLAK"
     gene            complement(7137..8651)
                     /gene="rpoN"
                     /locus_tag="VG272_09600"
     CDS             complement(7137..8651)
                     /gene="rpoN"
                     /locus_tag="VG272_09600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HWF13975.1"
                     /translation="MTFIMSWLQPKLHLRVAQKQILTPGLVQMVSVLALNRLELREMI
                     NQEMIENPVLEELSEESVTPEDYSAETFLKAETEKIPTTEPVSQFEDFDVASFFNQYL
                     DSGGGNPERGETEVIERPSFDKFLSSAQGLTEHLAWQMSVTVCNDVVREIAEAIIGNL
                     DENGYLSASLEEISQNGKYSMEDVEEALAVVQEFDPLGVASRNLRECLLTQLRTLDDQ
                     NTLAMQIVSECLPKRPLVASEAEELSAETPLARLLQANQLKEIARALNRPVELVKRAV
                     DVIKKLDPRPGLRYNKTQPRLVEPDVYFRKVDDQWQVFMNDDDVPQLRLSPVYRRLLA
                     RDAADREVRNYVKERVTAAMQLMKSIEQRKHTILRVCQIIIQRQGEYLDHGADRLKPM
                     MIKEVAEEVGVHPSTVSRAVSSKYAHTPQGVLELRSFFSESVNGPEGSNMSLQTLKRL
                     VKKMIDEEDTSHPLTDDLIAKKLDDAGIHVTRRTVAKYREDLRIPSTHQRRVKV"
     gene            complement(8648..9373)
                     /gene="lptB"
                     /locus_tag="VG272_09605"
     CDS             complement(8648..9373)
                     /gene="lptB"
                     /locus_tag="VG272_09605"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HWF13976.1"
                     /translation="MLKLQAVELSKSYRGRKVVDDVNLEISQGEVVGLLGPNGAGKTT
                     TFYILVGLARPDSGRVLLNGDDITDLPMYLRARNGISYLPQEPSVFRQLTVEENLIAV
                     LETLPLTSEQQRDRLEELLAQMKLETVRMNGAYTLSGGERRRLEIARSLVLEPAFILL
                     DEPFAGIDPLTVVDIQKIITELSREGIGVLITDHQVRETLNVTDRAYIVQNGKVLAAG
                     TPDDLSGNSEVRRVYLGDHFRLN"
     gene            complement(9380..11737)
                     /gene="lptC"
                     /locus_tag="VG272_09610"
     CDS             complement(9380..11737)
                     /gene="lptC"
                     /locus_tag="VG272_09610"
                     /inference="COORDINATES: protein motif:HMM:NF018537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HWF13977.1"
                     /translation="MRNSEAQKYARWSLAVAGLLALIVAGVYGRNTWLARQAEKKAPP
                     PVPSTVEERSNEFSYSKVEGQRTIYTVKASRTTAFKEGSRNLLEDVAIVVYGKAGDRN
                     DTLRTNACDFISNTGQINCAGDVLINMQAAGAAQAAANAVRVATSGLNFDRSSGVART
                     DKPVTFHWPAGDGRAVGVTYDSNTGTLRLVRNVELNLSTSSTGVSEKNKSGNGNDVHV
                     SGDSMFFQRAARIVQLVGNVHAQQAAIELTTEKLQLDLDAELQAKRIVASGHPQMHDV
                     TPDGPIALSADEIDSSMRSGGSVQSIVATGNVHATRETPVGANGIDAGRIQLDLATQN
                     NVPRLLTASKGVTLTSTNAAFNGGTRHVITDALEMHFAQPTRDGTTRLESVNTLAPAR
                     IDWQNVAIANGRSLPQVTRMAGKQMNLKFNAQNQLQQLVSTGGVEVNRKLGDQPAETT
                     ASRELIARFDKTGEWTTIDQTGDVHFHDAAYAGQGDRAHVDRASNIVTLDGSVLVADT
                     SMQTTAQSASFSSAPTMLHADGHVMTTDLHPSASSISNLAPQPAHISAEHLVADTMRG
                     HATYSGKARMWQGESVIEAETIELDRPAQVLVGKGRVRGVFPQAAWNPRAHDVTGAAS
                     ASLNLSKTPAPATSKTSAQRARSGSQLGHVRGGLLTYWEGESRGRIEQNARVDTEQGS
                     IQADRIDLYFSDSSAANGTKQLSRSVANGGVTVSQQDRRGTSDRAEYTASEGKFVLSE
                     GKPTLYSSTGDTTTGRQLTFFFADDRILVDSADGSRTVTLHQVEK"
     gene            11966..12391
                     /locus_tag="VG272_09615"
     CDS             11966..12391
                     /locus_tag="VG272_09615"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWF13978.1"
                     /translation="MKMHTYLNYGGNCREAFLFYEQHLGGKITMMVSHGEQPGLNSVP
                     ADLSKTILHARIIIGEAQLLGSDVPPQRFQPMRSAYLSLLVDSIDEAERIHALLADGG
                     EIFMPMQETFFAFRFSMLRDRFGTSWMILNERPAPQPAG"
     gene            12665..13381
                     /locus_tag="VG272_09620"
     CDS             12665..13381
                     /locus_tag="VG272_09620"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HWF13979.1"
                     /translation="MNFFDRDIIHFLNSFAGRFPLFDQSVAQISGNSFLNGGVMVALF
                     WWAWNSKSEFQTKYREYLLFGLIASVCAVFCARSLALTLPFRLRPMHNPALDFRVPIG
                     MNPDNLLGWSAFPSDHASLFFCFAAGLWMVSKRLGSIAFGYSLFVIAFPLMYLGAHQP
                     TDIIAGAILGIGLASLANVESLRKGITAPLVRWSDAYSGPFYAGLFLCSFEVAELFDS
                     VRKIGFTAYRDLETFLHTLR"
     gene            13526..14539
                     /locus_tag="VG272_09625"
     CDS             13526..14539
                     /locus_tag="VG272_09625"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWF13980.1"
                     /translation="MISIVAKFLSNFKISYNADEYISRKRSHVALPGGIHFAWLGFLF
                     IFVAGCGGVTGQPATSSTNQPAQPSQSVQFGHVFILVEENAEYSNVISNPAMPYLNSL
                     GMQNGIATNYYANGHPSIPNYFELTAGQTLTFDDGATPQGFPISADNVVHELIAAGKS
                     WKSYAESLPSVGYTGNDSGKYAVRHNPLAYFSDVQNSSSQAQNLVPFSQLASDLSAGT
                     LPQYSFIVPNLCDDAHDCSLTTADSWLQSNIEPLVKSATFQKNGLLIIVFDEGDTSDV
                     ANGGGHIPAVIVSPFAKHGFQSTTFYQHQSALRLSLEGLGVTKLPGDAAKAPAMWEFF
                     NAQ"
     gene            complement(14558..15016)
                     /gene="apaG"
                     /locus_tag="VG272_09630"
     CDS             complement(14558..15016)
                     /gene="apaG"
                     /locus_tag="VG272_09630"
                     /inference="COORDINATES: protein motif:HMM:NF003967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HWF13981.1"
                     /translation="MGIKEDWYRFAVRAECAIIAAPMFLSEATTRNIRVSVQSQYDPS
                     RSSPGNSQWFFLYTVTIANEGEETVQLVSRHWVITDGMGKVEEVQGPGVIGKQPTLAP
                     GQRFEYTSGCPLTTPFGSMHGTYQMINQTSEKFDIEIAPFTLSEPYTTVN"
     gene            complement(15707..16393)
                     /locus_tag="VG272_09635"
     CDS             complement(15707..16393)
                     /locus_tag="VG272_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13982.1"
                     /translation="MKPLEILGETLAPDGTILRLARRDTEYIILANGRSLMSSRMHGS
                     EEALAMLACARLRKMEEPCVLIGGLGMGFTLRATLDLLPPDAAVVVAEMVADVVEWNR
                     GPLGALAGYPMKDKRVRIEIGDVAATLKVSNGKFDAVMLDVDNGPTAFTSSANSWLYG
                     DRGIAAARLALKKNGMLAVWSARDDRKFEQRLRYGRFIVQVENVRARLKKGGPRHTIF
                     LGHKTESQGS"
     gene            complement(16550..16696)
                     /locus_tag="VG272_09640"
     CDS             complement(16550..16696)
                     /locus_tag="VG272_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13983.1"
                     /translation="MSSMFVRPAKKFRDGAASGNCSAASQALRKTADVRKLANEFDQK
                     LPEE"
     gene            16991..17686
                     /locus_tag="VG272_09645"
     CDS             16991..17686
                     /locus_tag="VG272_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13984.1"
                     /translation="MNGKWFQSSMARRKFLARLGVGVGAAGAAAVSSQAALPQAATDA
                     AFRPARHPQDDWYDKIPGVHRYVFDTSSAEGIGWALQFATNYFTASEQAYNLKDSDMA
                     VIIVARHKATSFGYNDAIWAKYGKQLSEQAEYIDPKTKEAPKINVYGPSGETLQAGKM
                     DGLIKKGAQFAVCSTSTRGIATRIAKANGMEVDAVIKEITSNLIGNARMVPAGVIAVN
                     RAQEHGYSFIFAT"
     gene            complement(17754..>18021)
                     /locus_tag="VG272_09650"
     CDS             complement(17754..>18021)
                     /locus_tag="VG272_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13985.1"
                     /translation="FEKPSSLVKITPVASAPVTKFRRVSIAALRQIKFLQILGFGLSG
                     AGFSLRGLVLARANFRRLKSLCENCKIGTSAAKAALILQTLCRG"
BASE COUNT         3923 a         5008 c         4798 g         4292 t
ORIGIN      
        1 tcataggtaa acagcgagcg cagcggaacc ggcaacgcaa cttcgcaata ggtggaggac
       61 atgtgcggag gttagtctag cccgaatttc acgtaagtag aaaagtagaa ctgggatgga
      121 actctgtgag gcaaccgaat tttgtagcgc cgccttcagg cgggctccgt gcatacaatc
      181 gcatgcacgc ctgaaggccg gcgctacatc taccctactt ggaaactttc ttcaccttta
      241 agccgagtag cgccatcact ttctgcaaat cgtcccacac ttcgcgcttc gcaggcgggt
      301 tgcgcagcag atacgcggga tggtacgtga ccatcagttt cgtgcctcga taatcaagcc
      361 attgaccgcg aattttggag atcgagtcct ttgttttcag gagcgactgc gcagccgtca
      421 ggccaaggca aacaatcgct ttcggcgcaa tggcgtcgat ctggcggtag agaaaaggcg
      481 agcaggttgc ggactcgtct tcttccggct tacgattctc cggcggacgg catttcacga
      541 cgttcgcgat gtaaacctga tcgcgcgtca gtcccatggc ctcaatcatt tgcgtcagta
      601 attttccggc gcggcccacg aacggtaagc cctgcatgtc ttcgtcgcgc ccggggcctt
      661 ctccaacaaa cacaagttct gcatgcgcac tgcccgcgcc gaacacaatt ttatttcgtg
      721 tggtgtgcag cctgcagcgc gtgcattcgc cgaggtcttc gcggatgcgc gtcagcgtat
      781 cgccttcgat gcgttcggcg atatcgaaca tggagggcac ggttgcaatg gggagcgccg
      841 gaagagttgc agcgatcttt acaggcgctt gcgtcatgac gggacgctcg acctcatgtt
      901 ttgcggtcgt cgctttgctt gcgagtgctt tcactggcgc tcgtttcttg ctcgcacgtt
      961 ttggcattgt tctccggtcg gtgtaaaacg cgccgaggtt taaatcgtcg taatagcgaa
     1021 gccaggcttc caatttttcg cgaggtgttt gcgggggtgt ggaatccatg ggcaatgggc
     1081 tgatcagcgc gtgcgctgcg tttcggcggg ctgttctagg gaatttccgg acgatggccg
     1141 taagccgcgc agatgatcga ggatgcgatg cgccgcgtca attttcggca tgcgttcaaa
     1201 tgtcttttcg ctgccgtcgc ggaaatacaa cgtgatgaca ttcgtgtcgt gatcgaagcc
     1261 ggcgccctct gccgtcacat cgttcgcgac gatcaaatct gcgtccttgg attcaagttt
     1321 ggcgcgcgca ttcgccgcaa cattttctgt ttccgccgcg aagccgacga ttacgcgatt
     1381 tgctttgccg cgcgacacat ccgaaagaat gtccaccgtc ggttccagct ctaaatttaa
     1441 gcggacggtg ccgcgcttca tttttttcgt ttgcggcgtt acgggccggt aatccgccac
     1501 ggcagccgcc atgatgagcg aagtagcgcc ctggaaattt tcgagcacgg cgcgatgcat
     1561 ctgctccgtt gtgcgcacgg aaataaattg cacgccttgc ggcggatcga gccgcgtcgg
     1621 cccgctgatc aagatcacgt ttgcgccgcg gcgtgccgcc gccgtcgcca atgcgtagcc
     1681 catcttgccg gaggagcggt tcgtgatgaa tcgaaccggg tcgaggtctt cgcacgtagg
     1741 gccggcggtt acgacgatgg tttcttcctg caaatcatgc gcgatgccga gccgcgcaag
     1801 gaccgcgctc gcaatcgcgt caacactggc aagccgcccc gcgccggtca tgccgcaagc
     1861 aaggtagcct tcctccggtg ccacgatgtg aactccgcgc tgccgcaaag cttccacatt
     1921 ttcctgcgcc gccgcatgct cccacatgtt cacgttcatc gccggagcga caaccacagg
     1981 cgcggtcgta gccaaataaa gtgtagtcag aaaatcgtcc gcaatgcctc gcgccatttt
     2041 cgcgattgtg ttcgcggtgg caggtgctat gaccagcaaa tcgattcgct gggccacggc
     2101 aatgtgctca atggcgcttt ccacgttcgc gccgccttcg gattccgcgc caaacatttc
     2161 ggtgattact ttttgtccgg tgagtgctgc aaaggtgaga ggtgtgatga attcctgggc
     2221 gctgtccgtc atgatcactt gcacttcaat gccgtcctgc tggagtttac gcacgagttc
     2281 cgccgccttg taggcagcca ctccaccgga gacgccaagt gctacgcgca taaacgtttc
     2341 ggcccgttaa tccttgcgcc gctttccatc tttgtccagc aaatcgcccg ggatatcggg
     2401 caattgatat tccagtacgc cggctctcaa ctcttccacc gccacgcgcg tggatttctg
     2461 agagtgcgga ttggctaaca gcggggacgc tccaagcatc aactgccttg ctcggcgcgc
     2521 ggccaccacc acaaatgcga atttgctatc cggtgcttga ctttctggcg ccataagttc
     2581 gttatcctcc gaaagattcc aatatgttgt gcgcgcgctg gctcaaacaa ctgcgaagac
     2641 accgcgcggc gatcgtgatg gcctggattt cgcgcccggc gcggtccatg tcgtcattaa
     2701 ccaccagaaa atcgtatgcc gtataccgtc gaatttcctg ctccgcacgt tccagcctgc
     2761 gcttgatcgc ctcttccgaa tcctgccctc gagcgcgcag ccgccgttcc aattcttcct
     2821 tggcaggcgg caatatgaaa atagccaccg catggggtaa cttctgtttt acctgttccg
     2881 caccctgtac gtcaatttcc aggaccaggt cgaggccctt gcattgcgct tcgtcaagcc
     2941 atcggcgcgg cgttccgtac tgatgctttc caaaaacgcg agcatgctcc agaaaatcac
     3001 ccgactgaac ccggcgttcg aattcatcct cggatacaaa atcataccat tttccggcac
     3061 tctccgtggc ccgcggcggc cgtgttgtgc acgatacgga aaacattgtg cccggcactt
     3121 ccagcatttt ttggacgagt gtggattttc ccgacccgga tggcccagat acgatgaaca
     3181 cgagcggcgt ttgcgcgtgt gccagtgtca ttcgacgttc tgcgcctgtt cacgcagctt
     3241 ctcaatatcg gatttaactt ccagcgccaa tcccgtaatc gccaggccct cggactccac
     3301 tcccggtgtc tttgacagca atgtgttcgc ctcccgttgc atttcctgca aaagaaaatc
     3361 gagcttcttt cctgcttcac ccactccggt tagcaatttg ccgaactgct cgacatggct
     3421 gcggagccgg gccagttctt cgctgacgtc gctgcgctca gccaatatgg aagcctcttg
     3481 cgccagacgt atgggatcga cctgcacgcc cttcagaagc tcgccgagcc gtatttccag
     3541 tcgatgcgcg atcgccggcc gcacccgttc ggtcagtgtc tcgatctccg ctgttcttgt
     3601 gccaatccgt cccagcattc cctgcatctc atccaaaagc gcttgacctt ccaggcgccg
     3661 catcgcctct aacttattta gcgcctgctc gaggcacgct tcgacttgcg cgcccagccg
     3721 ttcctgcttt tcttcgcttt gcaactcacc cgcggttccg ggcgcggcaa ccacgccggg
     3781 aagccgcagt agagaaacta aatccggctc ggcttttacg ctgaattcct tacgcaatcg
     3841 ctcgacagcc ctcaagtagg attcggcaat ttcgtgattc acttcgattg cgcccgttcc
     3901 gcccgcttcg taataaatgt ttatatcgac atggccgcgg cgcaaacggt ttcgaacagc
     3961 ctggcggatg cgcgattcga acacttcaaa tccatcgggc atgcgaaggt gcaggtcgag
     4021 gaagcggtga ttcacgctgc gcaggttgat gcgcacggcg cttccttcgc gctcgacgcg
     4081 cgcttgcgca aaaccggtca tcgagtaaat tccctcagtt gccgttgtag ccttcatcga
     4141 tccgccttgg gcgcgggagt cgccgcgtca tccatgtccg caaattgcat ttcgtaaagc
     4201 cgggcgtaaa ttccgctgcg agcgagaagt tcctgatgcg ttccggtttc cgcgattttg
     4261 ccgtcgtcca ggacgataat tttgtcggct cgccggatcg tggagagccg gtgcgcgatg
     4321 acgaacgctg tgcggtccat catcaagttc gaaagcgcgc gctgcaccag catttcggac
     4381 tcagagtcta actcagacgt ggcctcatcc agaatcagga tgggcgaatc cttcaggatc
     4441 gcgcgtgcga tggcgatacg ttggcgctgc ccgccgctca ggcgctgtcc acggtcgccc
     4501 agcatggtcc ggtagccctg cggcagttcc gcaatgaatt cgtgggccag cgctgctttg
     4561 gcggccgcct cgacgcgttc cttgggcagt cccggctggc cgtagcaaat gttattccag
     4621 accgtatcgt taaacagaat ggtctcttgc gtcacgatgg ccatttgttc gcgcaaggag
     4681 cgcagcgtaa cttcgcgcac gtccatacca tcaattcgca aaatgcccga cgtcaccggg
     4741 taaaaccggg gcagaagatt taccagcgtg gttttccccg caccgctcga tcccacaatc
     4801 gcgatcactt cgccggcgcg agcctgcaaa gaaatttcac ggaggattgg cgtttcagta
     4861 tcgtatccga agctgactcg atcaaagtca acgctgcgcg agaatggagg aagcgtgggc
     4921 aatccaggtt cggcggccgg ttctccctgg ctgccaatat attcgaaaac ttttgcggtc
     4981 gcgccgaatg ccagttcgaa ttgctggtaa accgtgccaa gtccctttac aggctcataa
     5041 gacttgaaca gcgcatagat gaacgtgacg aaaattccgg cggtcattgc gcctgtttta
     5101 atttgtacgc gcgcgtacat gagtaccagc acaatgacga ccgcgccaag caaatccatc
     5161 agtggggacg tggccaccgc cgcgcgaatc cagcgcatgt tttctcgcag caaacgctcg
     5221 gccgagactc gaaactttcc gatctcaaaa tcttccatgc caaatgcttt tacgacgcgg
     5281 ttgccgctga ccgtctcttc caaaatttgc gacagctcgc caatgcctgc ttgaattttt
     5341 tcagtcgagc gccggataca ttttccaagg gttcggacag gaacgaccac catcggtaaa
     5401 agaatgatcg agcccagcgt catcttccag ctggtcgcga acaggacggc aagaaggaag
     5461 aacagcgtaa aaatatggcg aaacccgaga gccagatatt cagaaaaagt gttgcggacg
     5521 cgttcgacat cgttgattac ggtggaaagt aaacgcccag tctggtggtg ctggaagaaa
     5581 ccgatgggct gccgaataac cttcgagtac acttcgtttc tcaggtccgt tactccagca
     5641 atgcctgcat gctgaaccaa cacgtttccc aaaaattcag cgaagccctt gccgataaaa
     5701 acagtcagga tggcaatcgc aaaaagagtt ccgacactgt gaatgctcga ggggagaaac
     5761 gaatttaaat aaatagcctg atgcgtcagc ggatttttga tgagcagaag cttggaatcg
     5821 ggagcctggg gattgagcac gcggtcaaaa atcggagtga tcatcagcgc gatcaaacct
     5881 tccgcgaggc ctactacgga aaggcacacg accccagccg caagccgcat tttgtacggc
     5941 atcacgtaac gcaataagcg gccgagttct tcccgtttat tcaaaaaatt attgatcccc
     6001 tgtgagatga acaaatatgc cgggccttgg tacgctacga atcgcccgcg cgtttgtcaa
     6061 gccgccgcgc ccttgagcca tgcaccctct gcgcggaaaa aacgtgccct ctccgtccct
     6121 aaaagcgtgg aacggagagg ccactacgaa accgactcac ttggccatcg ctggttttgt
     6181 agtggccgct cgtcttttgg gcgggcagct tttcctttgc ttcctgtatt tcctaaactt
     6241 ccttcacttc attacttcgc caggtcacgg tgcactacct tggatgcgta tccttttttc
     6301 tggagcgttt tgcgcacggc ttcggcgagc attacggaac gatgacgtcc gccggtgcag
     6361 ccgaaggcaa tcgtcaaata gctttttcct tcccggatgt aatgcgggat taaataaatc
     6421 aaaaggcctt cgacgcggcg caaaaattcc tcggtttgcg ggaaggaccg gatgtatcgc
     6481 gcgacgcgcg gatcgcgtcc ggaatatttt cgcaacttcg gaacgaagtg ggggttgggc
     6541 aaaaatcgta cgtcaaaaac aaggtccgca tccgccggaa ggccgtgtct gtagccaaaa
     6601 cttacgagcg aaaccatcat ggggcggcgg tcagtttttt gaaaactgcg cgtgatgaac
     6661 tggcgcagtt catgaacatt aaattttgtg gtgtcgatga ccacgtcggc gagcttgcga
     6721 atcggctcca tcaacacgcg ctcgcggcgc aggccctcca gcaccggacg gtcatgcccg
     6781 agcggatgcg gccgacgcgt ctcgctgaag cggcgcaata gcgcgtcctc cgtcgcttcg
     6841 atgaacacaa gcgttgctgg atcttccgcg cgcaactgcc gcaaaatggc cggcaacttt
     6901 tccagttgat cgccctcacg cacgtccacc agcagcgcag cgcgctgaat ttctccgccg
     6961 gagcgcatgt gcaattcgct gaatgtgggg atcagtgcga cgggaaggtt gtcgacgcaa
     7021 taaaagccga ggtcttcgaa agttttgaga actgtacttt tacccgagcc gctaaggccg
     7081 gtgagaatca ccagttgtcg ggggtcgcgc gcgcttctgc gcttcacgcg gccagcctat
     7141 accttgaccc ggcgctggtg cgtgctggga atgcggagat cttcgcgata tttggccacg
     7201 gtgcggcgtg taacatgaat tccggcatca tccagtttct tagcaatcaa atcgtcggtg
     7261 agcgggtgcg aagtatcttc ctcgtcgatc atcttcttca cgaggcgctt caaggtctga
     7321 agcgacatgt tgctgccttc cggcccgttg actgattcgg agaagaacga tcgcagttcg
     7381 agcacgcctt gcggcgtgtg cgcatacttg ctggaaacgg cgcggctgac ggtggaggga
     7441 tgcacgccca cttcttccgc aacttccttg atcatcatgg gcttcaagcg atccgcgcca
     7501 tgatcgaggt attccccttg ccgttgaata ataatctggc agacgcgcag aatcgtgtgc
     7561 ttgcgctgct cgatgctttt catcagctgc atcgctgcgg tcacgcgttc cttcacatag
     7621 ttgcggactt cgcgatcggc ggcatcgcgc gccagcagcc tgcgatagac aggcgagagg
     7681 cgcagctgcg gcacatcgtc atcattcatg aaaacttgcc actggtcgtc cactttgcgg
     7741 aagtacacgt ccggctcgac aaggcgcggt tgcgttttgt tgtagcgcag gccggggcgc
     7801 ggatcgagtt tcttgatcac gtccacagcg cgcttcacga gttccacggg gcggttcagc
     7861 gcgcgggcaa tttcttttaa ttgatttgct tgcaggagtc gtgcgagagg agtttccgca
     7921 cttagctctt cggcctcgct cgctaccaag ggcctcttag gcaaacactc ggaaacaatc
     7981 tgcatggcca acgtgttttg atcgtccagc gtgcgcaact gcgtcagcag gcattcgcgc
     8041 aaattgcgcg aagcgacgcc cagcggatcg aattcctgta ccacggcaag ggcttcctct
     8101 acgtcttcca tcgagtactt tccgttttgc gaaatttctt cgagcgaggc actcaagtaa
     8161 ccgttctcat ccagatttcc gataatggct tccgcaatct cccgcaccac gtcattgcaa
     8221 accgtcacgc tcatctgcca ggcaagatgt tcggtaagtc cctgcgcgga ggaaagaaat
     8281 ttgtcaaatg aaggacgctc aataacttcc gtttcgccac gctccggatt gccgccgcca
     8341 ctatcgagat attggttaaa gaaggaggcg acatcgaaat cttcaaactg gctgaccggc
     8401 tcggtagtgg gaattttttc ggtttcggct ttgaggaaag tttccgcgct gtaatcttcc
     8461 ggggtgacac tctcttcgga cagctcttcg agtaccggat tctcaatcat ttcctgattg
     8521 atcatctcgc gcagttcgag gcgattcagg gccagcacgc tgaccatctg gaccaggcca
     8581 ggggtaagga tctgcttctg cgctacccgt agatgaagtt ttggctgaag ccagctcata
     8641 ataaacgtca attcaaacga aaatgatctc cgagatacac acggcgcact tcggaattgc
     8701 cgctgaggtc gtcaggcgtt cccgcagcaa gcactttgcc gttttgaaca atgtaagcgc
     8761 gatcggttac attcagggtt tcgcgcacct ggtgatcggt gattaggacg ccgatgccct
     8821 cgcggctcaa ttcggtgata attttttgaa tatccacaac ggtcagcggg tcaatgcccg
     8881 caaatggctc gtccagcaga atgaaggcgg gctcaaggac cagcgaacgc gcaatttcca
     8941 ggcggcgacg ttcgcctccc gataacgtat aagcaccgtt cattcttaca gtttccagct
     9001 tcatttgcgc caataactcc tcaaggcggt cacgctgttg ttccgaggtc agaggaagcg
     9061 tttcaagcac cgcgatcagg ttctcttcga ccgtaagttg ccggaaaacc gaaggttctt
     9121 gcggcaaata actgattcca ttgcgggccc ggaggtacat ggggaggtcg gtgatatcgt
     9181 ccccgttcag gaggactcgc ccggaatccg gccgggctaa accaaccaga atataaaacg
     9241 tagtcgtctt cccagcgccg ttgggcccta acaggcctac aacttcaccc tggctgattt
     9301 ccaggttgac gtcatctaca accttacgcc ctcgatacga cttactgagt tcgacggcct
     9361 ggagcttgag catgccacgt catttctcga cctggtgaag cgtcaccgtt ctcgaaccat
     9421 ccgccgaatc tacaagaatc ctatcatcgg caaaaaagaa ggtcaattga cggcctgtgg
     9481 tcgtgtcgcc agttgagcta tatagagttg gtttaccttc cgaaagaaca aacttgcctt
     9541 ctgaggccgt atactcggct cgatcggaag ttcctcgacg gtcctgctgg ctgacggtaa
     9601 ctcctccgtt cgccacagaa cgtgataatt gcttcgtgcc atttgcggca ctactgtcag
     9661 aaaaataaag atcgatacga tccgcttgga tagatccttg ctcagtatct actcgcgcat
     9721 tctgctcgat acgcccacgc gattcacctt cccaatacgt cagtaatccg ccgcggacat
     9781 ggcccaactg tgagccggag cgtgcccttt gcgctgaagt tttgctcgtt gcgggcgctg
     9841 gggtcttact gaggttgaga gaagcgcttg ctgctcccgt cacatcgtgc gccctcgggt
     9901 tccacgcagc ctgcgggaaa actcctcgca ctcgcccttt ccccaccagg acttgcgccg
     9961 ggcggtccag ttcgatcgtt tccgcttcga ttacggattc accctgccac attctggcct
    10021 tgcctgagta ggtggcatgg ccgcgcatcg tatctgccac gagatgttcg gctgaaatat
    10081 gcgcaggttg cggggcaaga ttggaaatac tcgacgcaga gggatgcaaa tccgtggtca
    10141 tcacgtgccc gtcagcgtgc agcatggttg gcgctgaaga aaaggacgcc gattgggccg
    10201 tcgtttgcat cgacgtatcc gcgaccagaa cagagccatc gagagtgact atgttgctgg
    10261 cacggtcaac gtgagcgcga tcgccttgtc cggcatacgc ggcgtcgtga aaatgaacgt
    10321 ctcccgtttg atcaatcgta gtccactcgc cggttttgtc gaacctggct attaactcgc
    10381 gcgatgccgt ggtttccgcg ggctggtcgc cgagtttgcg attcacttcc acgccgcccg
    10441 tgctcaccag ttgctgcaat tgattttgcg cgttgaattt cagattcatc tgcttgcccg
    10501 ccatccgcgt cacttgagga agtgacctgc catttgctat agccacgttt tgccaatcga
    10561 tacgcgccgg agccagggtg ttcacgcttt ccaatcgtgt cgtgccgtcg cgggttggtt
    10621 gcgcgaagtg catttcgagc gcatcggtaa tgacgtggcg ggttccgcca ttaaatgccg
    10681 catttgtgga tgtgagggtg acgcctttac tggctgtcag caatcgcggc acattgtttt
    10741 gcgttgcaag atccagttga atgcgcccag catcaattcc gtttgcgccg acgggagttt
    10801 cccgtgtggc gtggacgttt cccgtggcca cgatggactg cacggaacca cccgacctca
    10861 tgctcgaatc gatttcatcg gcgctcagcg caatggggcc atccggagtt acatcgtgca
    10921 tttgcggatg gccgctggct acaatgcgct tcgcctgcaa ctccgcatcg agatccaatt
    10981 gcaatttttc ggtggtcagt tcgatggcgg cctgctgcgc atgaacattt cccaccaact
    11041 gcacgattcg cgccgcgcgt tgaaaaaaca tgctgtcgcc cgacacgtga acatcgtttc
    11101 catttcccga cttgtttttt tcggaaacgc ccgtggacga ggtggaaagg tttaattcaa
    11161 cgtttcgaac gagccgcagc gtgccggtgt tcgaatcgta agtcacgccc acggcgcgtc
    11221 catctcctgc cggccaatga aaggtgacgg gtttatcggt tcgcgctacg ccggaactgc
    11281 ggtcaaagtt cagaccggaa gtcgcaaccc gcactgcgtt tgcggctgcc tgcgcggcgc
    11341 cagcggcttg catattgatc agaacatctc cggcgcaatt aatctgcccg gtattcgaaa
    11401 taaaatcaca cgcgttggtc cgcagcgtgt cattccggtc tcccgctttt ccgtaaacta
    11461 cgatcgctac gtcttccagc aggttccggc ttccctcttt gaaggccgtt gtgcgagaag
    11521 ctttcacggt gtagatcgtc cgctgcccct ccacttttga ataggaaaac tcgttagagc
    11581 gctcttcgac ggtcgatggg acaggcggcg gggccttctt ctcagcctgc cgtgccagcc
    11641 atgtattgcg cccgtagaca cccgcaacga taagggccag cagacccgct actgcgagcg
    11701 accaccgcgc atatttctgc gcttcactgt tcctcatccg ttcgaaagcc tcactgccta
    11761 ggatgcgagt ataggggaca cgttgtcggc gaacaacgtc acgtccgccg atcgattcgt
    11821 cagcgcaatt tcggttgcat ccccattgga tgcacgatga tggattgcag gacgacgatt
    11881 tcaggacgat ggttgcctga agcggataac gttgtgttgt gaatctttgc aggaaatgtt
    11941 aaatggccga agggagatca ataacatgaa aatgcatacg tacctgaatt acggcgggaa
    12001 ctgccgagag gcattcctct tttacgagca gcatctcggc ggaaaaatta cgatgatggt
    12061 atcccacggc gagcagcctg gcttaaacag cgtgccagct gatttaagca agacgatctt
    12121 gcatgcccga atcatcattg gagaggcgca acttcttggc agcgacgtcc ctccgcaaag
    12181 attccaaccg atgcgcagcg cctatctttc gctgctggtc gatagcatcg acgaggccga
    12241 aaggattcac gccttgctgg cggacggcgg tgaaattttt atgcccatgc aggaaacttt
    12301 ttttgcattt cgattcagca tgttgcggga tagatttgga acttcgtgga tgattctcaa
    12361 cgaacgcccg gcgccacaac cggcgggatg acgggcaaat cggaggccgt tgtcataccg
    12421 gaaaattgat tgcaaattca tggttttatc gttgcaagaa agcttgacgg aaaaccggat
    12481 tacgctataa atatttgaaa tgcaggatag gactctctcc atgcgccatc caggtggctt
    12541 ggagaatgat tcatcagcca gaagagtcct ttcgcattcc ccgtcccccg gcgaatgcga
    12601 aaacgagtga acatcaattt agttttagac ttacagatgg acttcggaga agggtgcgtg
    12661 ctcgatgaat tttttcgatc gagacattat ccactttttg aattctttcg cgggacgttt
    12721 tcctttattc gatcaatcgg tagcgcagat ttcgggaaat tcatttctga atggcggcgt
    12781 gatggtggcc ctgttctggt gggcatggaa ttccaagtcg gaattccaga ccaaataccg
    12841 cgaatacctt ctgttcggat tgattgcttc cgtttgcgcg gtattttgcg ccaggtccct
    12901 cgcgctgacc ctccccttcc ggctgaggcc gatgcacaat ccggcactgg attttcgcgt
    12961 gccgatcggg atgaatccgg acaatttgct cgggtggagc gcttttccga gcgatcacgc
    13021 cagcctcttt ttctgctttg ccgccggact ctggatggtg tccaaacgcc tgggttctat
    13081 cgcgtttggc tactctctat ttgtgattgc atttccgctg atgtatctgg gcgcgcatca
    13141 acccaccgac attatcgccg gcgcaattct agggattggc ttggccagtc ttgccaatgt
    13201 cgaatcgctc aggaaaggca ttacagcgcc attggtacgc tggtccgatg cctattcggg
    13261 gcctttttac gctgggttat tcctttgctc gtttgaagtc gcggaactat tcgattcggt
    13321 acgcaagatt ggatttacgg cgtatcggga ccttgaaact ttcctgcaca cgcttcgata
    13381 attgtgccct cggggttcag ttccatgttt aaattttcgt ctgctataat ttaattcgct
    13441 cccctcttgc cttgcagacc tctcctttcg gagaaagcaa aggacccaat gacaaatgca
    13501 gcgtcaactc aacgaaacgc gtctcatgat ttccatcgtc gctaaattcc tctccaattt
    13561 taaaattagc tacaacgctg atgaatatat ttcgcgcaag cggtcgcacg ttgccctgcc
    13621 tggtgggatt catttcgcat ggcttggctt cctttttatc tttgtggcag ggtgtggagg
    13681 cgtcactgga cagccggcaa cttcctccac gaatcaacca gctcaaccga gccagagtgt
    13741 acagttcggc catgttttca tcctcgtcga agaaaacgcg gaatattcca atgtgatctc
    13801 aaatcctgcg atgccctatt taaacagcct gggcatgcaa aatggaatcg ccacaaacta
    13861 ttacgcgaac ggacacccat cgatcccaaa ctattttgag ctcaccgcgg gacaaacgct
    13921 cacattcgac gacggcgcaa cgccgcaagg tttcccaatc tccgcggata atgttgtcca
    13981 tgaactcatc gccgcgggca aatcctggaa atcatatgca gagagcctgc cgtccgtagg
    14041 ctatacggga aacgatagcg gcaagtatgc ggtccggcac aatccgctcg cctactttag
    14101 cgacgtgcag aacagcagct ctcaagcgca aaacctggtt ccgttcagtc aactggccag
    14161 cgatctctcg gcgggaaccc tgccgcaata ttcattcatc gttccgaatt tatgcgacga
    14221 tgcgcatgac tgctccctga ctacggcaga ttcctggctt caatccaaca ttgaaccgct
    14281 ggttaagagc gcgacgtttc agaaaaatgg cctgctgatc atcgttttcg atgaggggga
    14341 tacatccgat gttgcaaacg gaggcgggca tattccagcc gtgatcgtca gtcccttcgc
    14401 aaagcacgga tttcaatcga ctacttttta tcagcatcaa agcgcgcttc gcctttcgct
    14461 cgaaggtctc ggcgtgacga aactgcccgg agatgcggcc aaagctcccg ccatgtggga
    14521 attttttaac gcgcaatgaa ggaagatgcc tgcggcgcta gtttaccgtg gtatagggtt
    14581 cggacagtgt aaacggagca atctctatat cgaatttctc ggaggtctga ttaatcatct
    14641 ggtaggtacc gtgcatggag ccgaatggag tggttagcgg gcaacctgat gtgtattcaa
    14701 atctctggcc tggagccagc gttggctgct tcccgatcac gccgggaccc tgcacttcct
    14761 cgactttgcc cattccgtcc gtgataaccc agtggcggct cacaagctgc accgtttcct
    14821 cgccctcatt cgcaatcgtc acggtgtaga gaaaaaacca ctggctgttg cccggactgg
    14881 aacgcgaagg gtcgtactgc gactgaacac tcacgcggat gtttctggtt gtggcttcgg
    14941 ataagaacat tggcgctgct ataatagcgc attccgcgcg gactgcgaaa cggtaccagt
    15001 cttctttaat tcccacgact tcatccgcaa ttgagaatta ttcttcgata tgtcacgaat
    15061 tcaaaaagtc gattttgcga ttcggcggaa cagatttata atggtgtatc gacattctgt
    15121 gtcgagaagg aggtgatcca gtgaggtacg gaacaatttg atttggttgt aggagaggcg
    15181 cttgtgaatg tgccgagcgt ctctcttttt tttgcccctc caatcccgca aaccgccaaa
    15241 ttccttagaa tcaatgaatg ataagaagct cgaagattga aaatttcaat tcgtcaatga
    15301 ttgagagcat tcgcttgagt taaaacctca atgtgaatcg atgaaatcaa atgcgatttg
    15361 aatttatgaa gaggaaatag gtgaatacat ttccatcaca gttttttgaa agttgactca
    15421 tactcgctgt ggtgacaaat tctatattcc gatgattgaa cgttttccgg cttgacgacc
    15481 gcaaaggcaa aaatgaactc attaaaatgc tgcaaatgtg gaaagcgaac tcgcaagtta
    15541 acggtggtct gcgaaaattg cggacatcga ttctgcaaag tatgcaaagg cgacaaaggg
    15601 tgaccctgcg cgcgtcatac atgtgggtga gaagtcctct ttaaaattca ctgttttttt
    15661 caagctgttc gcttggattc gccaattgca gcagttgaat gcgggttcag cttccttgtg
    15721 attccgtctt gtgacccaga aaaatcgtgt ggcgcgggcc gccttttttc aagcgcgcgc
    15781 gaacgttctc cacctgaacg ataaagcgac cgtagcgcag gcgctgctcg aatttccggt
    15841 cgtctctggc agaccaaaca gctaacatcc cgtttttctt gagagccaga cgcgcggccg
    15901 cgattccccg gtctccataa agccaggaat tggcggatga ggtaaatgcc gtgggaccat
    15961 tgtcgacatc gagcatcacc gcgtcgaact ttccgttgct cactttgagg gtagcggcaa
    16021 catctccgat ctcgatccgt acgcgcttgt ctttcattgg atatccggca agcgcgccca
    16081 gcggaccacg attccactcc accacatcgg cgaccatctc cgcgacaaca accgcggcat
    16141 ccggtggaag caaatccagc gtcgcacgca acgtgaagcc cattcccagt ccgccaatca
    16201 gcacgcaagg ctcttccatt tttcgcaggc gcgcgcaagc caacatggcc agcgcttctt
    16261 ccgagccatg catccggctc gacatcaggc ttctgccatt agccaaaata atgtactccg
    16321 tgtcgcggcg ggcgagcctt aggatggtgc cgtcgggcgc aagcgtctcg ccaagtattt
    16381 ccagaggctt cacgaaattg tgctcatggt tgaagaaaaa ctaaacgcgt ctttgcgacg
    16441 aaatatgaat cggtattgaa aatacgatca tgctgccatt cggcgggatt cgcaatcaca
    16501 agatgcggcg gccacgttga ttatttctgc ggatggacac gaactgagac tattcttccg
    16561 gcaatttttg atcgaattcg ttcgccaact tgcgcacatc ggcggttttg cgcaaggctt
    16621 gcgaagccgc gctgcaatta ccacttgctg ccccgtcgcg gaactttttc gccggccgca
    16681 cgaacatgga cgacatgcaa attttggtac tgtcctaagt tgaagaaatt cgcgtggcac
    16741 ggttccttta ggagggcggg acttcagtcc cgacgacaag cttggtttta tctgggtttt
    16801 aacccctgaa gcttcacggg ctgaagcccg cgacgaaagg caacccaaat ggccgagttg
    16861 aaaactcggc cctcctaaat tcgagaaatt ttcaaattag gacactaccc aaaattttga
    16921 aaattccaaa tctttgaaac ttgggcttat actagggcca cgatctgcca cggaaaaggg
    16981 gaatatccat atgaatggaa aatggtttca atcctcgatg gcgcgacgaa agtttctcgc
    17041 acggctgggc gtgggagtcg gcgctgcagg cgcggctgcg gtaagttcac aggcggctct
    17101 tcctcaagcc gcgactgatg cggcatttcg gccggcacga catccgcaag atgattggta
    17161 tgacaaaatt cccggcgtgc accgctacgt attcgatact tcatcggcgg aaggcatcgg
    17221 ttgggcattg caatttgcca ccaactattt caccgcgagt gagcaggcgt acaacctgaa
    17281 agatagcgac atggccgtca tcatcgtggc gcgtcacaaa gcaacatcat ttggttataa
    17341 cgacgctatc tgggcgaagt atggcaagca actttccgaa caggccgaat acatcgatcc
    17401 caaaacaaaa gaagctccaa aaattaatgt gtatggtccg tcgggtgaaa cgctgcaagc
    17461 cggaaagatg gatggcctga ttaaaaaagg cgcgcagttt gccgtttgtt cgacgtccac
    17521 tcggggcatc gcaacgagaa tcgcgaaggc aaatggcatg gaagtagatg cggtaattaa
    17581 ggaaattact tccaacctga tcggcaatgc gcgtatggtt ccggcgggag tcattgccgt
    17641 gaatcgggcg caagagcacg gctactcgtt tatttttgca acgtaagtgc gattcagtag
    17701 cgcgggactt cagagtctgt gtgagaacag tagaatcatt gtgggtcgtg gctttagcca
    17761 cgacataagg tctgcaaaat gagtgcggct ttagccgctg aagttccaat tttgcagttc
    17821 tcacacagac tcttcagcct gcggaagtta gcccttgcaa ggactaaacc ccgcaggctg
    17881 aagcctgcgc cacttaaacc gaatcccaat atttgcaaaa atttgatttg ccggagggca
    17941 gcgatgctaa cgcgacgaaa cttcgtaacg ggagcactcg ccacgggagt tatcttgacc
    18001 agcgatgatg gtttttcaaa a
//
LOCUS       DASZMB010000210         8841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_206107, whole genome shotgun sequence.
ACCESSION   DASZMB010000210 DASZMB010000000
VERSION     DASZMB010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8841
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206107"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /locus_tag="VG272_09655"
     CDS             <1..1389
                     /locus_tag="VG272_09655"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWF13986.1"
                     /translation="RVITHVENIIKAVPEVENTSRRTGLQLGLAAVTEPNTGDIAVKL
                     KDKRSRGIDEIISEVRGKVTTQEPALDVEFTQVLQDMISDLTGAPQPVVVQLFSDDVD
                     QLTVWAPRVADALDRIQINYKKPVVDIEDGIENTTSGPAVVFKINPAAAAKAGFTTDQ
                     LTTIASAIVDGEPAAAPMIIGDRPYTLRVRYAQPSRASLEAMNNTTIVNGNGGAATLG
                     SVSTFEEVPGQTEILRDNLQQEKEVTARLEGIDLGRGIAAVQKAVIDLHLPPSIRVAY
                     GGTYKEQQKSFGDLVTVLLLALVLIFIVLLFEFRAFSAPVAILSSAVLSTSGVFFALF
                     ITRTDFNVSSFMGLIMVLGIVAKNGILLLDANHKFIIAGFEPEEAMIQAGRRRLRPIM
                     MTAMAAVAGMLPLSLALGAGSQMLQPLAIAVIGGILISMILSLIITPAIQYYLTRNKH
                     AVVSNTAPVAVR"
     gene            1496..2551
                     /locus_tag="VG272_09660"
     CDS             1496..2551
                     /locus_tag="VG272_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HWF13987.1"
                     /translation="MSPKKKAGWMRKIGPMAGLALLASVVVYAAPQAGGYHVIKRMPV
                     GGEGGWDYMTVDPDTHRLYVARGSHFMVVDDTTGKVLGDIEIANSKNSHGMALALELG
                     KGFTSNGDANTVSVVDLKTLKATTVISIPGKDPDSIRYDAATKRVFTFNGKSMDSTVI
                     DATSNKVIGTVKLAGKPEEAVVDGGNMWVNIEDKSVIQEFDIKSYAIKGSWPLAPCDG
                     PSAMALDSAHHRIFVACDKVMVVVNSENGKVVAAPPIGGDPDGNGYDPGTGMAFASCR
                     DGFVSVIHQDSPDKYSNVGNINTQAGTRTMALDTKTHRVYTETSDFKPAAAPTPDNPR
                     PRPTPIPGSFVILEVGQ"
     gene            2920..3864
                     /locus_tag="VG272_09665"
     CDS             2920..3864
                     /locus_tag="VG272_09665"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWF13988.1"
                     /translation="MNYSSIRRALAGPAALLIVSMIATAPARAQAPVDDSKTVVKSNK
                     ITDNFYTVDGRGGTIGVLVGSQGTFMVDTQFAPLTDRIVAVIKQISPNPIRYVVNTHV
                     HPDHTGGNANMAKMGATIFARDELRERLMNPPPGPNGQPGMAPAPDAALPIVTYEGKV
                     TFHMDGETIDLIPIRRAHTDGDTMVYFHNADVLMTGDFYRSIGYPNIDRVNGGSLNGM
                     IQGLSEVIALAGPNTKIIPGHGAIVNRNAVMAHRDMILGVRDQVAKLSQQGKSSDEVI
                     AAHPTAAFDNNVLPAAPNGDRMADRFVGQVYAELKTGK"
     gene            4036..4500
                     /locus_tag="VG272_09670"
     CDS             4036..4500
                     /locus_tag="VG272_09670"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HWF13989.1"
                     /translation="MPPFILESLQQLNTYVGKEIATTGWLQMTQQRIQQFAETTGDTQ
                     WIHIDEQRARRESPYGTTIAHGFLTLSLLTHFMREAIQLPGEVRQTINYGLNRVRFPA
                     PVRTGEKIRAHIGLQSCKNLSGSVEAVFQITIEAENSTKPCCVAEWILRYFY"
     gene            5031..5885
                     /gene="purU"
                     /locus_tag="VG272_09675"
     CDS             5031..5885
                     /gene="purU"
                     /locus_tag="VG272_09675"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HWF13990.1"
                     /translation="MRQSAILRVQCPDAKGLDAALAEFIYRAGGNILHFEQHMVHELR
                     LYLARIEWDLSGFQMEMKDFAQHFTPIADRMDMKWQIALSGDRDRVAIFVSKYDHCLV
                     DLLYRWHTGELSCDIPLIISNHPDAKRHADFYGIPFHVIEINRENKAEAERRQCELLE
                     ENKIDLIVLARYMQILSPAFIADHGRRIINIHHSFLPAFVGAKPHHQAFARGVKLLGA
                     TAHYVTEALDDGPIIEQDVIRISHRDSLQDLVQKGRDLEKIVLSRAVSWHLEHRVLQY
                     ANKTVVFD"
     gene            6154..6927
                     /locus_tag="VG272_09680"
     CDS             6154..6927
                     /locus_tag="VG272_09680"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HWF13991.1"
                     /translation="MAAAQAFSAPLVSAQASAGSGQAAPQSTPAAPQQTPPAQAQPPA
                     EPPPPPQKTLAVVVLDPAHGGADPGARGSSGIAESDVVLAFARLVRISLEAQGFRVIL
                     TRQANEDPSFDDRSKVSNAQRGNIFITLHVSSTGQPGTVRVYSMPRMALPQNGAVAPR
                     GGLLPWDRAQFNFVDQSRRLAELIQIQMAQKFRGSSETPFEAPVRQLRTVAAPAVAIE
                     ISSVSVPSRTPLDQMGPGLADGVARAVAAFKIIYESGGR"
     gene            6932..7627
                     /locus_tag="VG272_09685"
     CDS             6932..7627
                     /locus_tag="VG272_09685"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HWF13992.1"
                     /translation="MSKGLKTTILILSAMVVVGLIALPGLRQAIQRLQSPPKTEEQSR
                     REVMDEPISTPTDVQVQAQMFWLSASSPNSLEATAIQLPLSADPVERSRQLLNALILR
                     APSAEKRTIPAQASLLAFYLQPDGTAIADFSDEFSSAMPSGILSEQLAEQSIVQTLGA
                     NVAGIKQLKILVHGQEAEALAGHLDLYGFFPVSGVPAVTAPASGPAALSSATPHATTL
                     AGPTKTQSNPSAK"
     gene            complement(7749..>8841)
                     /locus_tag="VG272_09690"
     CDS             complement(7749..>8841)
                     /locus_tag="VG272_09690"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HWF13993.1"
                     /translation="ILSQPYHDRANCTYCGFCQAFGCHVGAKSSILVTKLPEADATGN
                     FTLLTGTMCYQVRTDNSGRATGVSYYGPDGSDNTIEADLVILSPFIYDNTRLLLLSKT
                     AKFPNGLANSSGQLGKHVTAHIMAKVFAAYDDRFVNIYMGPSAQKHTIDDFNGDNFDH
                     SGLGFMRGAQISVAPADLEAGPIGTAMSMDPPPGIPKWGAAYRDFLGKYFTRYAAINA
                     QTENLAYADQTIDLDPNVRDQYGMPAPRVTYDWRRPNELARIEFLMQKMEGIGKAMGA
                     TKVWRGALSPGAPGAHHEGGTRMGNDPKTSVVNRYGQTWDIPNLFVIGSSTFPSMSGF
                     NPTLTIQALAYLSADAIVKRYKSKPGPLV"
BASE COUNT         2000 a         2393 c         2472 g         1976 t
ORIGIN      
        1 cgagtcatca cgcacgtgga aaacatcatc aaggccgttc ccgaagtcga gaacacttcg
       61 cgccgcactg gcttgcagct tggacttgcc gcggtgaccg aacccaacac cggcgatatc
      121 gcggtcaagc tgaaggacaa acgcagccgc ggaatcgatg agattatcag cgaggtccgc
      181 ggaaaggtga cgactcaaga gcctgctctg gacgtcgagt tcacccaagt gctgcaagac
      241 atgatttcgg acctcactgg cgcccctcag cccgtcgtcg tgcaactctt ttccgacgac
      301 gtcgatcagc tgacggtatg ggccccacga gtggctgatg cattggaccg catccaaatc
      361 aactacaaaa agcccgtggt cgatatcgaa gatggtatcg agaatacgac cagcggcccc
      421 gcagtcgttt tcaagatcaa tccagcagct gccgccaagg cgggattcac cacggaccaa
      481 ctcaccacga ttgcatcagc catcgttgat ggtgaacctg cggcggcacc gatgattatc
      541 ggcgatcgcc cgtacacgtt gcgtgtgcgt tacgcgcagc caagccgtgc ttcgctggaa
      601 gcaatgaaca atacgacgat cgtgaatgga aatggcggag ccgccacgct gggttccgtc
      661 tccacgtttg aggaagttcc cggccaaacg gaaatcctgc gcgacaattt gcagcaggaa
      721 aaagaagtga cagcgcggct tgagggaatc gatcttggcc gcggtatcgc tgcggtgcaa
      781 aaagcggtaa tcgatttaca cttacctccg tcgattcgcg tagcctacgg cggcacttac
      841 aaggaacagc agaagtcatt cggggacctg gtcaccgtgt tgctgctggc cctggtcttg
      901 atcttcatcg ttctgctgtt tgaattccgg gccttctcgg cacccgtggc gattctttcc
      961 tcggcggttc tgtcgacatc cggagtgttc ttcgctttgt tcattacacg caccgatttc
     1021 aacgtctcgt ccttcatggg gttgatcatg gtgcttggaa tcgtggccaa gaacggtatt
     1081 ctattgctcg acgccaatca caagttcatc atagctgggt ttgaaccgga ggaggccatg
     1141 atccaagccg gccgccgaag gcttcgtcca attatgatga cggctatggc cgccgtggcg
     1201 ggtatgttac cgttgtccct cgcgttgggt gcagggtcgc aaatgctgca acccctcgcg
     1261 atcgcggtca tcggaggaat cttgatctcg atgatcctgt cgctgatcat tactccagca
     1321 attcagtact atctgacgcg caataagcac gccgtggtca gcaataccgc accagttgcg
     1381 gtgcggtaag cgaaagggcc gcgatgaaat ttattgcggc attgaaacgt attagtgaat
     1441 aaggaccggg tatgtttgcg ttctgctcga acgaaaccat aaggagatga ttcgtatgtc
     1501 accaaagaaa aaagcaggat ggatgcgcaa gattggcccc atggccggtc tggcactgct
     1561 tgcgtccgtg gtcgtatacg cggcgccgca ggcgggaggc tatcacgtca ttaagaggat
     1621 gccggttgga ggcgaaggcg ggtgggatta catgaccgtc gaccccgata cgcatcgcct
     1681 gtatgtcgcc cgaggatcgc atttcatggt tgttgacgat acgaccggaa aagtgctcgg
     1741 cgatatcgaa attgcaaact cgaagaactc gcacggcatg gcccttgcct tggaactcgg
     1801 caaaggattc acaagcaacg gagatgccaa cacagtttcc gtcgtggacc tgaagacact
     1861 gaaggcaact acggtgatct ccatccccgg aaaagatcct gattcgatcc gctacgacgc
     1921 ggctaccaag agggttttta catttaatgg caagagcatg gattccacgg tgattgacgc
     1981 gacctcgaac aaagtgattg gaaccgtgaa gctcgccggc aagccggaag aagctgtcgt
     2041 cgatggcgga aacatgtggg tcaatatcga agataagagc gtgatccagg aatttgacat
     2101 taagtcctat gcgattaagg gctcctggcc gctcgcgcct tgcgacggac catccgcaat
     2161 ggccttggac agcgcgcatc accgtatttt cgtggcctgc gataaggtca tggtggtggt
     2221 gaactcggag aacggtaaag tggttgccgc tccgccaatc ggcggcgatc ctgatggcaa
     2281 cgggtatgat ccgggcacag gcatggcatt cgcctcctgc cgtgacggtt tcgtttccgt
     2341 gattcaccag gattcaccgg ataagtactc caacgtcggg aacatcaaca cccaagctgg
     2401 tacccgaaca atggcgctgg acacgaagac gcaccgcgtc tacaccgaaa cctcggactt
     2461 caaacccgcc gccgctccta caccggacaa tccgcgaccg cggccaactc cgatcccggg
     2521 atcgttcgtg attctcgaag tcggacagta attagctact cggaattaaa aacgcccctg
     2581 tgctcgaacg agtccagggg cgttttcttt ttgtggtgta ggcttccggc tgtgggtttc
     2641 atgttgctgt agcccgcctc ttcagaggcg ggggtttaga ttccacgggc ggcagggtcg
     2701 acgtgtgaaa tctaaatccc cgcctctgaa gaggcgggct acagtagtgt gccaaatccc
     2761 agcatgaccg gcagaatcgt tacccaaaat agtttcaatg caacttccag accaccaaat
     2821 gaattccttc gcctgagcat tggtgtaaca cgcgtttggt atatactttc gaacagcaac
     2881 ccaagtcaaa atcgcatttt ccatcaggag caaacatcaa tgaactattc atcgattcga
     2941 cgcgcccttg ctggcccggc ggccttgctg attgtttcca tgattgcgac ggcacccgca
     3001 cgcgctcagg ctccggtcga cgacagcaaa acggtagtga aatcaaacaa gattacagac
     3061 aatttttata cggtggatgg ccgcggcggc acaattggcg tgctcgtcgg gtcccagggc
     3121 acattcatgg tggatacgca atttgcgccg ctcacggatc gaatcgttgc ggtgatcaag
     3181 caaatttcgc ccaacccgat tcgctacgtg gtcaacacgc acgtgcatcc cgaccacacg
     3241 ggcggcaacg caaatatggc caagatgggc gcaacgattt tcgcgcgtga cgaattgcgc
     3301 gaacgattga tgaatcctcc ccccggtccc aacggccagc cagggatggc gccagctccg
     3361 gacgccgcgc ttccgattgt cacctatgaa ggtaaagtta cgtttcacat ggacggagaa
     3421 acgattgacc tcatccccat tcgcagggcg cacacggatg gcgatacgat ggtttatttt
     3481 cacaacgccg atgtgctgat gaccggcgat ttctatcgct cgatcggcta cccgaacatt
     3541 gatcgcgtaa acggcggctc gctcaacgga atgattcagg gactttctga agtgattgcg
     3601 ctggccggcc cgaacaccaa aattattcca ggacatggtg ccattgtgaa cagaaatgca
     3661 gtaatggcac acagggatat gattctgggc gttcgcgacc aggttgcgaa actcagccag
     3721 caaggaaaat catcggacga agtcattgcg gcgcatccca ctgctgcgtt cgacaataat
     3781 gttttgccag ctgctccgaa cggcgatcgc atggcggatc gtttcgttgg tcaggtttac
     3841 gccgaactaa aaaccggtaa gtagcagtcc agcgacgcgg gcttcagcct gtgaggtttg
     3901 gcactttgct gcggcccgcc tcttcaaggg tggaggttta gacgacgcga catctaaatc
     3961 cccgcctctg aagagacggg ctacaatagc atcaaacgtc gcaggctgaa gcctgcgcta
     4021 cataaatcaa aatccatgcc tcctttcatt ttggaatccc ttcaacaatt gaatacgtat
     4081 gtgggaaagg aaattgccac gaccggctgg ctgcagatga cgcagcagcg cattcagcaa
     4141 ttcgcggaaa ccacaggaga cacgcagtgg attcacatcg atgagcagcg cgcgcggcgc
     4201 gaatcgcctt atggaacgac aatcgcacac ggatttttaa cgctttccct gctgacccat
     4261 ttcatgcggg aagcgattca gttgccaggg gaagtgcggc aaaccattaa ttacggcctg
     4321 aatcgcgtgc gattcccggc gccagttcgc acaggcgaga aaattcgcgc gcacatcggc
     4381 ctgcaatcct gcaaaaattt atcgggtagc gttgaggcgg ttttccagat cacgattgag
     4441 gctgaaaatt ccacgaagcc gtgctgcgtt gctgaatgga ttttgcggta tttttactag
     4501 gagtcatccc caaaatggca tgtcttccaa actcgggagt gcgacagggg ctaaagtcca
     4561 tgtttttcag gtccctagat gtcgcggctg aagccgcgac ccacaaagat tatttatgag
     4621 atagctcggc tctattgatg tcctgcatgc tgcaggaaga ttcgcccgat ttgtgtagca
     4681 ggaattgtag ctcgcctctt cagaggcgag ggtttaggtg aaagtgtgac caaaatttcc
     4741 tgctcagcgc gtgcgcccgg ggttccaggc cgcggaccaa ccttcgacac ccttatttca
     4801 ggctgtgaga agatcatcgt ctaaaccccc gcctctgaag aggcgggcta caacagatcg
     4861 gcagcggaac cgggatgtca acagagccag atagcttcga aataaaatgt ctgccaagcg
     4921 atttttccaa ccacattcgt aaaaatatca attagaaatg ggcccgaagt tgctgcctct
     4981 tctagagcga ctttttactg tacaatttgt ccgccatggg aggagaaatc gtgagacaga
     5041 gcgccattct gcgcgttcag tgcccggacg ctaaaggcct ggatgccgcg ctcgctgaat
     5101 tcatttaccg cgccggcggc aacattctcc attttgaaca gcatatggtc cacgaactga
     5161 ggctttacct ggcgcgaatc gaatgggatc tgtccggctt tcagatggag atgaaggact
     5221 tcgcgcaaca cttcacgccg attgccgatc gcatggatat gaaatggcag atcgcgctgt
     5281 ccggcgaccg cgatcgcgtg gcaattttcg tttccaaata cgatcattgc ctagtggact
     5341 tgctttatcg ctggcacacc ggcgagcttt cttgcgacat cccactcatt atttccaacc
     5401 atcccgacgc aaaaaggcac gcggattttt acggcatacc cttccacgta atcgaaataa
     5461 accgcgaaaa caaagccgag gcggaacggc ggcaatgcga attgctcgaa gaaaataaaa
     5521 tcgatctgat cgtgctcgcc cggtatatgc agattctttc tcccgcattt attgcggacc
     5581 atggaaggcg aatcatcaat attcatcatt ctttcctgcc cgcattcgtt ggcgcgaagc
     5641 cgcatcatca ggcgtttgcg cgtggcgtaa aattgctcgg agcgacggct cattacgtta
     5701 cggaagcgct ggacgatggc ccgattatcg agcaggacgt gatacgaatt tcgcatcgcg
     5761 attcgctgca ggacctggtg cagaaggggc gcgacctcga aaaaattgtc ctttcgcgcg
     5821 cggtgagctg gcacctggag catcgcgtgc tgcagtatgc caataagacg gtcgtattcg
     5881 attaggttta ccttttcaaa attacaagtt gccgcgcaaa tttccgggta tggtttgctg
     5941 tagcccgcct cttcagaggc gggggcttaa acgacgggac atctaaatcg ccgcctctga
     6001 agaggcgggc tacactagca ccaaacccca caggccgaag cctatgccac atcagaccaa
     6061 attttcgggc acgacttctc tcatgaacga aatgattctg gtatcctcgt aatagtgccc
     6121 agcaaatcga tttgcctaat ttttgtgtgc gtgatggccg ctgcccaggc gttttcggcg
     6181 ccgctggtat cggcacaggc atctgcaggc tcaggtcagg ccgcgccaca atccacgcct
     6241 gccgcccctc agcaaactcc tcctgctcaa gcacaaccgc ctgctgagcc gccaccacct
     6301 ccgcaaaaaa ctttggccgt cgtggtcctc gatcccgcac atggaggcgc tgaccccgga
     6361 gcccgcggtt catcgggtat tgctgagagc gatgttgtgc ttgcttttgc acggctggta
     6421 cgaatttcgc tggaggcgca aggatttcgc gtcattttga cgcgccaggc gaatgaagac
     6481 ccttcgttcg atgatcgttc gaaagtttcc aacgcgcagc gagggaatat ttttattacg
     6541 ctgcatgttt cctccacagg acagccggga acggtgcgcg tgtattccat gccgcgcatg
     6601 gccttgccgc aaaatggcgc tgtcgcgcct cgcggcggcc ttctgccgtg ggaccgcgca
     6661 caatttaatt ttgtcgatca aagccgcaga ttggcggagt tgattcagat ccagatggcg
     6721 cagaaatttc gcggatcctc tgaaacgcct ttcgaagcgc ctgtccggca actacggacg
     6781 gtggcagcgc ccgcggtggc aattgaaata tcgagcgtca gcgtgcccag tcgcacgccg
     6841 ctcgatcaga tggggccggg actcgcggat ggagtggcgc gagcggttgc cgcatttaaa
     6901 attatttatg aatcgggagg caggtagcgc gttgtcaaaa ggactcaaaa caacgatttt
     6961 aatcctgtcc gctatggtgg tcgtgggcct gatcgcgctg ccaggattgc gtcaggcgat
     7021 tcagcggctg cagagccctc ccaagacgga agagcagtct cggcgtgaag tgatggacga
     7081 accgatctcg acgcccactg acgtgcaggt gcaggcgcaa atgttttggc tgtcggcctc
     7141 atcgcccaat tcactggaag ctacggcgat tcagctgccg ctttccgccg atccagtcga
     7201 gcggtcgagg caattgctga acgcgcttat tttgcgggcg ccgtctgcgg aaaaacgcac
     7261 gattccggca caggcgagct tgctcgcgtt ttatcttcag ccggacggga cggcgatcgc
     7321 ggatttttct gacgaattct catcggctat gccatcggga atcctaagtg agcagcttgc
     7381 ggagcaatcg attgtgcaaa cgctgggcgc gaatgttgca gggattaaac aattgaaaat
     7441 tttagttcac ggacaggagg cagaagcgct tgctggacat ttggacctgt atggattttt
     7501 tcctgtctcg ggggtgccgg ctgtgactgc accggcttct ggtcccgccg cgctttcgtc
     7561 agctacacca catgcaacga ctcttgcggg acccaccaaa acacagtcca atccttctgc
     7621 aaagtagctc agaaattact tcgattggta atttcgaacg gggaatttcg tggcatgggc
     7681 ttgagcctgt ggggttttat gctggcgcat atggaacccc acgggctcaa gcctatgtca
     7741 cgtagaagct aaaccagcgg gccaggcttg ctcttgtagc gcttcacgat tgcgtccgcg
     7801 ctcaggtagg cgagagcttg gatggtgagc gtgggattga agccgctcat cgatgggaac
     7861 gtggaactgc cgatcacgaa aagatttggg atgtcccacg tttggccgta gcgatttaca
     7921 accgaagttt tgggatcgtt gcccatgcgc gtgccgcctt cgtggtgcgc ccccggtgct
     7981 cccgggctca gcgcgccgcg ccacactttt gttgcgccca tcgctttgcc gattccttcc
     8041 attttttgca tcaggaattc aatgcgcgcg agttcgttcg gcctgcgcca atcgtaggtg
     8101 acgcgcggtg cgggcatgcc gtattggtcg cgcacatttg gatcgaggtc gatggtctga
     8161 tcggcgtagg caagattttc agtttgcgcg ttaatggctg cgtaacgcgt gaaatatttg
     8221 ccgaggaaat cgcgatacgc cgcgccccac ttggggattc cgggaggcgg atccatgctc
     8281 atagccgtgc cgattgggcc ggcttcgagg tctgcaggag caacggaaat ttgtgcgccc
     8341 cgcatgaagc ccagaccgct gtgatcgaaa ttgtcgccgt tgaaatcgtc aatcgtatgt
     8401 ttttgcgcgc tcgggcccat gtaaatgttc acgaagcggt cgtcataggc ggcaaaaact
     8461 tttgccatga tgtgcgctgt cacatgcttg cccagctgtc cactggaatt ggcaaggccg
     8521 ttggggaatt tagctgtctt cgacagcaga agcaggcgcg tgttgtcata gatgaatggc
     8581 gagagaatca ccaagtccgc ttcaatcgtg ttgtccgagc catctggacc gtaataagaa
     8641 acgcctgtcg cgcggccgct attgtcggtc ctcacctgat aacacatcgt gcctgtcagc
     8701 aatgtaaaat tgccggtcgc gtcagcttcg ggaagcttgg tgacgaggat gctggatttt
     8761 gcgccgacgt ggcatccaaa tgcctggcag aaaccgcagt acgtgcaatt cgcgcggtcg
     8821 tgatagggct gggaaagaat c
//
LOCUS       DASZMB010000211        30636 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4881751, whole genome shotgun sequence.
ACCESSION   DASZMB010000211 DASZMB010000000
VERSION     DASZMB010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 30636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30636
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4881751"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..951
                     /locus_tag="VG272_09695"
     CDS             109..951
                     /locus_tag="VG272_09695"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HWF13994.1"
                     /translation="MAITMDDPSVDIGPFMTWPEANRRLLDTFSQRSLKIALFVCGMR
                     VDHSEGQKLLTDWDKAGHLICNHSYSHLNFNGPKVTYERFASDFARNEPILQPYSRRR
                     NLFRYPALQEGDTAEKRDRFRALLKSRGYKNGYVTIDASDWYVDQRMRERLSKDSTAA
                     MAPYRDYLIAHLLDRANFYRQLALDTLGHEIPHTLLVHYRPLEAFFLADVMTAFEKVG
                     WEWINADQAFDDPVFLREPQTLPAGESLVWALAAESGQFKDRLRYPGEDDAYEKPKMD
                     ALGL"
     gene            1292..1693
                     /locus_tag="VG272_09700"
     CDS             1292..1693
                     /locus_tag="VG272_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13995.1"
                     /translation="MKRLIIFVASVIAASVCLTAKAPAVRAQSCDTAPAPDCPYGYYD
                     SPPYDCAPYGYYGPEWFPSSIFVGAGPWFNGPDKFHGFVDNHLDPQHGYKGPNPKRGE
                     KPDASKHPDKNPDFKGNEVRDGRGHVVEDKR"
     gene            2409..3926
                     /locus_tag="VG272_09705"
     CDS             2409..3926
                     /locus_tag="VG272_09705"
                     /inference="COORDINATES: protein motif:HMM:NF017493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HWF13996.1"
                     /translation="MEFLAGSLLDLITETSTNLPPDVRAAMSIAAKEETPNTQSAQAL
                     GIILSNIDMAVDDTGPICQDTGMPTFYVHTPVGVNQIRVKKAILEAVAEATRLGKLRP
                     NSVDSLTGKNSGNNLGEETPVIHFEQWEEDDIEVKLILKGGGCENKNIQYSVPCTLDH
                     MGRADRNIEGVRKCILHAIWQAQGQGCAPGAVGVCIGSDRAHGYELAKGQLFRTLDDV
                     NPNPTLAKLEAEIMEEANQLGVGAMGFGGNVSLIGCKIVAANRLPASFFVSVAYDCWA
                     FRRLGVRLDAKTGAINSWLYRDPERAVQRMAASSGFQLSGHEVVLTAPLTEAQMRSLK
                     VGDLVLINGEMRTGRDNVHAYLMKNPPPVDLNGSVLYHCGPVMLKQGDDWVVKAAGPT
                     TSIREEPYQADVIKKYGVRAVIGKGGMGAKTSAALKEFGAVYLNAIGGAAQYYARTVE
                     KVLGVELMEFGIPEAMWHLRVKNFAAIVTMDAHGNSLHADVEKATGEVLETLQKV"
     gene            complement(4021..4668)
                     /locus_tag="VG272_09710"
     CDS             complement(4021..4668)
                     /locus_tag="VG272_09710"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF13997.1"
                     /translation="MNRERHHSAGENITEISMDEKTEDQLIELGLNGDARALDALFAR
                     NTRALYQTAFRVLGNPEDAEEALQEGLLSAYRNLPRFERRSQFSTWLTRIVINAALMR
                     RRSKRSRPAVSLDDFALDGELPLADRFADDGPNPEQLYAGTELGDRMKKKLAEISPLL
                     RTAFWLREIEGLSAEEAAHVLGVSRNTLKARLWRARQELAARLGLALDTNLAPAT"
     gene            complement(5369..7447)
                     /locus_tag="VG272_09715"
     CDS             complement(5369..7447)
                     /locus_tag="VG272_09715"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HWF13998.1"
                     /translation="MSEVPIPVAGSTSPEARHQALLEVAEAIAQHRDLGELFHELAER
                     LHRVVEFEYLNLILYDPLRNVMRLHILESEKQFRKYGPGSEFSVGETPSGWVWESQKP
                     YLLDDIEKETRIPDIVQTLRENGVRSFCSLALTTAQRRLGVMSFGRGTQHIHTESEVA
                     FLQQVARQVAVAVDNALNFESAQAYQRQLARERDRLRVLLEVNNAVVSKLDLHVLLNA
                     IAASLRRVIHHEYTSLAVFEPETDSMRVLALNFPEGKGLIREDLLVPVERALAGKAFR
                     THHAQVLDRNEIEAFDSVTSRLMMVEGVRSLVCMPLITHDRALGTLSLASLRDSAFQQ
                     GDVDLLAQVASQVAIAVENALAFQEIAELKNKLAQEKLYLEDEIRSEMNFDEIIGESA
                     SLRAVLKQVETVAPTDSTVLITGETGTGKELIARAIHNLSPRRERTFVKVNCAAIPTG
                     LLESELFGHERGAFTGAIAQRIGRFELADGGTIFLDEIGDIPLELQPKLLRVLQEQEF
                     ERLGSTHTTHVDVRMVAATNRNLAEMVAARTFRSDLYYRLRVFPILMPPLRDRQEDIS
                     SLVRYFVEKHARRMNRKVETIPAETLEQLVRYPWPGNIRELENLIERAVIVSPGPVLR
                     VPLSELKSGGEALVESQTLRAAERDHILKALETTKWVLAGPRGAAVRLGMKRTTLQSK
                     MRKLGIEKQK"
     gene            7647..8123
                     /locus_tag="VG272_09720"
     CDS             7647..8123
                     /locus_tag="VG272_09720"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF13999.1"
                     /translation="MHKVKSTMALNVHKKFRQGQILKLVSSEPISSQDELRRRLSHMK
                     VRVTQGTLSRDMSELKLVKTADGYKPLTNGDEFAAPMPPLGRALREFLVDLRPAFNLL
                     VLKTPPSGAQPLAAALDAEKFKEVAGTIAGDDTVLVITPSRQARAAVQKRMEDLLR"
     gene            8120..9154
                     /gene="argC"
                     /locus_tag="VG272_09725"
     CDS             8120..9154
                     /gene="argC"
                     /locus_tag="VG272_09725"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HWF14000.1"
                     /translation="MNDPQRVAVVGATGYAGFELAQLLLRHPKIQKPTFYLRESSAHV
                     KCLTEIFPHLRGWGEAPCKTFSVDAIASSGASTVFLATPHEVSLETVPQLLNAGLRVV
                     DLSGAFRFHNPETFSQWYKLPAPPRNLAEEAVYGLPELYADQLPGARLVANPGCYPTS
                     VILGLRPLVESGWIATERGIVADCKSGATGAGKEPKKEMQYVEVDENFRAYGLFSHRH
                     TPEVTEHTGIPGEDIVFTTHLLPVSRGILSTLYVWFSEPHTAQEVESLYRAFYADRPM
                     VRIWPAGKLPELQHVAHTNFSDIGFALDPAGRRLAVVSCLDNLGKGAAGQAVQNMNLM
                     MGIGEATGLR"
     gene            9151..9969
                     /gene="argB"
                     /locus_tag="VG272_09730"
     CDS             9151..9969
                     /gene="argB"
                     /locus_tag="VG272_09730"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HWF14001.1"
                     /translation="MRLVVKVAGALLDDAAAMETLARQVSQLAHQGHEILVVHGGGKI
                     FTATLKQMGIESKFVSGLRVTDRATRDVAVMVLGGLLNKRLAGAISAAGQPAVGICAS
                     DASCFLAEPMMHDEVVGGLGFVGYLTGVNLEFLESFWKAGIIPVASCMGLGADGELYN
                     INADHMAAACAEYIHADRLIYLTDVAGVLDGSKVVKVVSTGDAEDMIRQNKVSGGMIL
                     KLEACKRALSAGVSEVRIVGGSVPKGLLAAANGARFPGTRITAEFPVTSAGAAQ"
     gene            9966..11276
                     /locus_tag="VG272_09735"
     CDS             9966..11276
                     /locus_tag="VG272_09735"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWF14002.1"
                     /translation="MKPKAKKSATKVKPASAATKSILNDASQHLMNTYRRSEMIFTHG
                     RGCYVFDQNGNKYLDFLGGIAVNALGYSHPALVRTIRREAGRAVHVSNLFHNPFQGPL
                     AAKLAKWSGMDRVFFTNSGAEAVEGALKLARIAAKQKPGGATKTRFLAMEHSFHGRTF
                     GSLSITHPVKYREPFEPLIPGVEFVRLNDVADLESKFTDDVCAVIVEPIQGEGGVYPV
                     TNEFWKRARALATLHGAVLIADEIQCGLGRTGKAFAYQGLEGLPDIVIVAKPLAGGLP
                     LGALLAREEVAASFSPGLHGSTFGGGPLTCAVGLTFLETVEKKHLLANVQKRGTELKA
                     GLKKLAAKFDFIREIRGEGLIIGIDLDVDGAPYVAEALKQGLLINCTHDHILRLLPPF
                     ILTAQQVKEGLSKIESVLAKTERPAPSAAVTSTPTEAQMLAAVR"
     gene            11279..12244
                     /gene="argF"
                     /locus_tag="VG272_09740"
     CDS             11279..12244
                     /gene="argF"
                     /locus_tag="VG272_09740"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HWF14003.1"
                     /translation="MNAQAPAQGQTESHTLSLSTDLLTGTEWNAAQVQELFRLSVDVK
                     AHPDRYKSALAGRFLAMIFEKPSLRTRVTFEVGITSLGGTAIYLDHADTHLGDRESVP
                     DVARCLSRWVHGIVGRVFSQDVLQILADYASVPVINALSDVYHPCQALCDFFTIEEKF
                     GSARGVTLAYIGDGNNVCHSLMVAGARVGAHVRIATPAGYEPDAKILASARVDAAATQ
                     GTIELFRAPEEAVTGAQAVYTDVWASMGQEEESEKRAAIFAPYQVNAKLMSQAASDAV
                     FLHCLPAHRGSEVTDDVMESPRSIVFDEAENRLHVQKAILLMLLS"
     gene            12256..13452
                     /locus_tag="VG272_09745"
     CDS             12256..13452
                     /locus_tag="VG272_09745"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF001770.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HWF14004.1"
                     /translation="MPKKVVLAYSGGLDTSIIIPWLKETYGGCEVIAMIGDVGQQEDL
                     KAAKKKALATGAASATIEDLREEFLTEYVWPTLRAGAVYEHKYLLGTSMARPVIAKRQ
                     AEIALETGADAVAHGCTGKGNDQVRFELAYKAIAPKVKIISPWREWTINSREDAIAYA
                     NAHKIPVEQTKANIYSRDRNIWHLSHEGGVLEDPANEPDEAMWQWIASPEKAPNKPAL
                     VEIGFDGGTPVSVNGKKRSAMVLLNELNEIAAEHGVGRIDLVENRLVGIKSRGAYETP
                     GGTLLITAHRELETLCLDRETAHYSEILSLRYAELVYYGTWFTPQREALDAYFTEAQK
                     RVTGTVGLKLFKGNVTVTKRVSPYSLYRKGLASFAMTGYNPKDAEGFINLFALPVTIP
                     AESKVK"
     gene            13449..14870
                     /gene="argH"
                     /locus_tag="VG272_09750"
     CDS             13449..14870
                     /gene="argH"
                     /locus_tag="VG272_09750"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HWF14005.1"
                     /translation="MTATPKEQPSGKLWGGRFDRAPDELFYQFQRSFSFDRRLLPYEL
                     AVDRVWAGALESAGILSVEEMRDTLTALDKIAGRAENEYAWLGGSPAEDVHHFVETAL
                     VEYLGALGYKLHTGRSRNELVATDFRMFVMESVGEMRVALSRLISALVGQAEKVLGIP
                     MPGMTHMQHAQPILFSHFLLAHAEAFFRDAARLDAAEAMADACPMGSGALAGCALDVD
                     RGAIARELGFARPTANSLDAASDRDFALDYLYALAVIATHLSRLAEDFVLFASQEFGF
                     IVLSDEFSTGSSLMPQKKNPDAWELLRGKTGRVTASLHTLLTTLKGLPSSYQRDLQED
                     KEPVFAAHDQAYAMLTIAAGAVAATRPNEARMREAASDPALLATEAAHYLVRRGAPFR
                     QAHEIVGQIVREGERAGKSFSTLPLEILQKFSPLFAPDFYDALKLESALAGPDVEGGT
                     APARVRDAIAAAKARLAALEKKS"
     gene            14867..15397
                     /locus_tag="VG272_09755"
     CDS             14867..15397
                     /locus_tag="VG272_09755"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWF14006.1"
                     /translation="MRARTARMSDAAAVHRVIAHYAGEGLLLPRTEAEIREHIARFIV
                     LVEKRGREEKVLGCVALEPYGADLAEIRSLAVAPEARGEGRNVGERLMKAAMDTARRR
                     KIARLFAVTHRPEFFSRYGFTPGPRQIVPEKIERDCATCPKERTCNLIATVAVVCPER
                     DLLPVVSGAGKVLSML"
     gene            15571..16815
                     /gene="argJ"
                     /locus_tag="VG272_09760"
     CDS             15571..16815
                     /gene="argJ"
                     /locus_tag="VG272_09760"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HWF14007.1"
                     /translation="MSDLPSSRALPRGFRFASASCGLKKRGHTGTLDVAMIFSDSPAS
                     AAAVFTQNLVQAPPVVLSREHVRTSADSMRAVIVNSGNANCSVGPGGMVASRATAETV
                     GKLLACRPDQIVVCSTGVIGVPLQVERILNAAPGLAKSLELSARAFDGLNRAIMTTDT
                     RPKRAAASCKIAGKTVRVAGCAKGAGMIHPNMATMLSFVVTDAAVAPAVLDSALREVV
                     NRTFNCITVDGDSSTNDTLLVLANGASGAKEITKTGGADYRIFVGALEKVCKNLAIGI
                     VEDGEGATHLVDIEVRSAPSDAAAKKVAETIALSALVKTAIAGADPNWGRILAAAGRA
                     GVPFNPEKAEIWLGKMKMYGPPKGSSYSVALPLNERAAHKRLLGKNVSILIDLHNGRG
                     AARMWTCDFTKDYVEINASYRT"
     gene            complement(16923..17864)
                     /locus_tag="VG272_09765"
     CDS             complement(16923..17864)
                     /locus_tag="VG272_09765"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HWF14008.1"
                     /translation="MMPEQNSEIPASQSPLGPPAGPRTGRKAIFWNETEFRAGWRLLM
                     YLFFVVIFGLVGTFLITVLHLPEISRSGVTATSMLIQEVTGIVATFMAAGIMSMLEDR
                     RFGTYGLPFSEAFGSRFWQGTVWGIAMITAIIFLIRAFGGFTFGELALHGSHLLGYAI
                     LWGVVFILVGIFEEFLFRGYAQCTLSMGLGFWPAAILLSAGFGAIHLHNTGEDKIGAL
                     SVFVIGMFFCLTLWRTGNLWFAVGLHAAFDWGETFLFSVPDSGLVAPGHFLNSSLHGP
                     AWLTGGLVGPEGSVMSFVVVGITAIIFIFIYPAKKTD"
     gene            complement(17869..18345)
                     /locus_tag="VG272_09770"
     CDS             complement(17869..18345)
                     /locus_tag="VG272_09770"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HWF14009.1"
                     /translation="MARIETSVQIAAPAEAVFAFFVPQRMPYWYGADMQTCLEVQGGA
                     PDFAVAQKVHIAGNLGKREVSHTAVVTALQQARLFEWRFSDRYGVKGSERWELESLTV
                     PSAHNSLGTVVRMISDYTMPGFLGKMMDWLMTRHAVARRNENYLARLKRYAEHGPE"
     gene            complement(18469..19305)
                     /locus_tag="VG272_09775"
     CDS             complement(18469..19305)
                     /locus_tag="VG272_09775"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HWF14010.1"
                     /translation="MSLQPVRVTVLMAALMLLGFVALRRTEFADSVRADLENNPSAAV
                     APDLAATPVLVELFTSEGCSSCPPADALLSRLGRTQPVHGADIIALEEHVDYWDRLGW
                     KDPFSSEAATVRQNDYASAFGGQQIYTPQMIVDGRAEFVGGSDNDALRAIRSASQSPK
                     PAVQLSWDTGDNLKINVSPLTVAMHGDDLQLLVAVAENMLHTDVKRGENAGRALEHND
                     VVRQLSLVAKINSPSSGFSSTTTLHPGHEWNRDNLRAVVLVQERHSRHILAVAAIPFS
                     KV"
     gene            complement(19388..20059)
                     /locus_tag="VG272_09780"
     CDS             complement(19388..20059)
                     /locus_tag="VG272_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14011.1"
                     /translation="MRNRIRELMLLSAVFVFTGLPVLAQDAMGTLSTTSSAASSEADP
                     APKPASWGSSQGRMFFPHNSIRGYADFEVAPSHNEPDLGRCTQSGALIATAGGANSPC
                     TAYARYLLGGYLEIQPFGKTLARHLFVFFTPTFSLGSNVPQFKYTASMSGIAFERSVG
                     VGFELPKNFEIRITQHQVDWLGKYRNKNIDLAVPGLDLGTSGPYGLYATIGARYYFGG
                     YGRAH"
     gene            complement(20102..21481)
                     /locus_tag="VG272_09785"
     CDS             complement(20102..21481)
                     /locus_tag="VG272_09785"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWF14012.1"
                     /translation="MNRRSRQIAQFFFVFLSFVAAGFFFAASGSAQDTQPPATPSTTS
                     PEQPSSPQVQGYTLTPDQEAKAIAYAHARHELYFLDAAYGLLLLVMILQLRVAPRYRD
                     WATQWTDSDFGQIVVFAPLLFITIDVLSLPIGIWAHRLALQYQQSIQGWNSWLSDWAK
                     GEGVEIAIASLMVWILFTVIRKSPRKWWFWFWIAAVPLIIFGAIVEPVIIEPLYYKFA
                     PLANAQPRLATKIEQMTIRAGAPIPQSRILEMTASAKLRQLNAYVSGLGASKRVVVWD
                     TTIRHMSEDEVLFVTGHEMGHYVLGHVRDGILFACAVLLFFLNLSYRSLNWMLARWGN
                     SWAIRGPDDLAALPVLLFLLSVFGFLFTPISNAYSRHLEHQADQYGLEVIHGLIPDAP
                     VVAAHAFQILGEVDLEEPSPSTAVKLWFYNHPPLDERMRFAQTYDPWSQGRAPEFVKG
                     TGDVASPSK"
     gene            complement(21627..22157)
                     /locus_tag="VG272_09790"
     CDS             complement(21627..22157)
                     /locus_tag="VG272_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14013.1"
                     /translation="MRKWFLTAIAAAFLSLASVSAGFAQQQPGEGRDPSAPDAPKPAA
                     NANLPDKPDKPDQKTNGGIAPTLNPVAPGVHKFWDRENDLLFAAVGAGRALDYASTLN
                     LRHRGINEAFLTNSIVDNRPLFAGIEIAATAASVGVSYVFHRTGHHRLERWTSAIHAG
                     VAVGGAIRNYNLKSPN"
     gene            complement(23154..23687)
                     /locus_tag="VG272_09795"
     CDS             complement(23154..23687)
                     /locus_tag="VG272_09795"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWF14014.1"
                     /translation="MAVVNPLTKEQAAPELKDTFEKLAGRAGKVPNIFAAMAHRPAVL
                     STFLPLYKAVVNQGTVEPKFKELAYLRASMVNGCEYCTRAHIASSKGAGVTPEQIAAL
                     PFYARSPLFDEKEKATILYADRVTRGASGIRSPALEGLRKYYDEGQIVELTLVICMAN
                     FTNRFNDATELIPDLGV"
     gene            23950..26808
                     /locus_tag="VG272_09800"
     CDS             23950..26808
                     /locus_tag="VG272_09800"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWF14015.1"
                     /translation="MVSSARQINAEADQERILSLVRELVAELGHESGVRSTGVHSHLD
                     RDLGLGSLERVELLVRLEKTFGTRLSEDVVAGAEIVQDLIDAVSTANGEPVNARLQDQ
                     NVSRAARTSFSGQAEGLPTAQTFEEVIRHRGRVDAAQTHLIFYEDEGESPTLTFGELL
                     AGAERVAADLARRGIGRGDTVALMLPTSRDFFLSFAGTLLAGAIPVPIYPPFRADRIS
                     EYAERQSAILANAGARLLVTFREAASAAKLLKPLVPSIESVVTADTLVRSDAAIPLGT
                     LLHSRANDLALLQYTSGSTGNPKGVMLTHANLLANVRSIGEALGMRNEDVGVSWLPLY
                     HDMGLIGAWLMPLYFGLPVVVMSPLAFLSRPARWLRAFHRFRGTIGAAPNFAYELAAA
                     KISDEDIQGVDLSPWRAALNGAESVLPATLNRFVARFAAYGFRRETLLPVYGLAEASL
                     AVTIPQVGRGPLVDRLDRTAFAQGGRAISVAPEIPAEDLNVISFVSVGPPVPRHEVRI
                     VNDRGENLPERTEGQLWFRGPSSTQGYYRNEAATAALLPEGLAAGWVNSGDRAYCADG
                     EIYITGRVKDIIIHAGHNLYPHEIEDCVARVPGVRKGCVVAFGATDPVAGTERLVIVA
                     ESRERNRSLREQLVQAITAQVMETLGQPPDKVEVVPPNAIPKTSSGKLRRDATKQRFL
                     SGKLYSGARPVWLQVAWLAAASSVVRIRGGVTRAREFIYGFYAIAMFALLLLPAWLYV
                     LISPSRKSSARVTTAALRAYLKVAGWQVHVEGREHLQHAKPRMFVANHTSFADIVVLM
                     AALGTDYHFVAKTEVMSMPFFGTFLRKLGHYSFHREDPRARLEQAKQVEEALHQGESV
                     FIFPEGTFNAQSGVRAFHLGAFKAATAAQREIVPIALDGTRRVLRDGTWLPRPGKISI
                     TICPPVIPPPSTEDWQQIVQMRDSIRETIATFAHEPLL"
     gene            complement(26960..27742)
                     /locus_tag="VG272_09805"
     CDS             complement(26960..27742)
                     /locus_tag="VG272_09805"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HWF14016.1"
                     /translation="MRENARQDHEKSEVMLGSCLVEGEISSASSARRTRREAFGISLF
                     IEILILGLLVAAPLFNSVAQPNFRPILPTQLSFFRPGTLPKRGPIDAAHAINRRSEIP
                     NPYHQLKYGVTVRRVQNEEGPGVPDAGFEGPYLPGGDMLPGGGVAQLIREPEPVAQPP
                     QVEKHPVKISEGVLQAQLITRVEPRYPALAMQTKTEGEVRLHAIINRDGRITSLDILS
                     GHPLLVHAALEAVSQWRYRPTMLNGEPVEVETSITVIFRLHN"
     gene            28021..28335
                     /locus_tag="VG272_09810"
     CDS             28021..28335
                     /locus_tag="VG272_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14017.1"
                     /translation="MRLPSKEAVGVRYTGWAALGRDPQEDTLEYACPYCGEPGVKAAR
                     RTPDQYHFTDGFLQDLQGEMAACRTCRHSLRIAPVVLLHDQDEKRRFEGVFSDDLIPS
                     KN"
     gene            complement(28468..29319)
                     /locus_tag="VG272_09815"
     CDS             complement(28468..29319)
                     /locus_tag="VG272_09815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HWF14018.1"
                     /translation="MKRRSHPKEIVPPYRDPVFMESSPARTVRFTTEYIDPLERLRRE
                     KVGDTIVMFGSARILSHDQATKHLHRLQEVQKQNLTPLQYKAALSDAKSALQMSRYYE
                     EARELSRRMTSWAMSLGEHPRRFVICSGGGPGIMEAANRGANEAGGKSIGLSIELPHE
                     QRPNDYISPELSFMFHYFFMRKLWFAQLAKALIVFPGGFGTMDELWEMLTLMQTGKLQ
                     RRNIILIYGRKYWDRVLDWKHMVRTGMISRQEYGMLEFADTVDQAFDRVRNGLEKHHM
                     EPDSLLV"
     gene            29549..>30636
                     /locus_tag="VG272_09820"
     CDS             29549..>30636
                     /locus_tag="VG272_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWF14019.1"
                     /translation="MRTPLIWLGTLTATIIFWSTPLHAQQTQGPISPKPGEPVQQSPE
                     GRIRVRVSLVSAPVVVVDAKGQPILDLQQKDFHITDEGVGQTIESFDLGGEPVAAVFV
                     FETSSRIQPLLPAIQKSAIVFTQTVVGPSGEAAVVSYDSSVNTLLPFSTDSDRIEKTI
                     ANLKAGNSGAHLYDALSSAVGLLRNGPIGKRRAIVVVGEANDDGSEQKLGRVLREAQL
                     ANIVIYSVGLSTTSASLRAPVTQSAPPTGTPPGTFGGPSFPGTAQTPTSQQQYGGNID
                     LLGLAEWAVRNATAVVKDRPLEVAATATGGTYESVFRDTKIETAIDEIGGELNSQYML
                     SYRPTSAENPGYHQIKVTVDRPGVKVRTR"
BASE COUNT         6903 a         8469 c         8534 g         6730 t
ORIGIN      
        1 gcaggaacga tcggagcaca gttcgcaagt tgcgcggatt cgagggagtt gtttgccgct
       61 ccgaagtcga aaagattatt ccagccgatc aagggcgggc gaccgcaggt ggcgattacg
      121 atggatgacc cgagcgttga cattggtccg tttatgactt ggcccgaagc caatcggcga
      181 ctgttggaca cattctccca gcgcagcctc aaaatcgcgt tgttcgtctg tggaatgcgg
      241 gttgatcact ccgaaggaca gaaacttctc accgactggg acaaggcagg gcacttgatc
      301 tgcaaccatt cgtattcgca cctaaatttc aatggaccga aggtaactta cgaacgattc
      361 gcgtcggatt ttgctcgaaa cgaaccaata cttcagcctt attcgcgcag gaggaacttg
      421 tttcgatatc cggcgctaca agaaggcgac accgccgaga agagggaccg ttttcgagca
      481 cttttgaaaa gccgcggcta caagaatgga tatgtcacaa tcgacgcatc tgattggtat
      541 gtggaccagc ggatgcggga acggctttcg aaggattcca ccgccgcaat ggcgccgtat
      601 cgggattacc tgattgccca tttattggac cgagccaatt tctatcggca acttgctctc
      661 gatacgctcg gccacgagat ccctcacaca ctacttgtgc attacagacc tctggaggca
      721 ttcttccttg ctgatgtgat gaccgcgttt gaaaaggtag ggtgggagtg gatcaatgcc
      781 gaccaagcat tcgatgatcc ggtttttcta cgcgaacccc aaacattacc ggcaggtgag
      841 agcctggtgt gggcactggc tgccgagagc ggacagttca aagataggct gagatatcca
      901 ggcgaagatg acgcgtacga gaaaccaaaa atggacgcgc tcggtctcta gagtacaaga
      961 accgagaggc agaaacttgt gccctgagga ccttggtggg caaagttcca cattccgata
     1021 accccgctct cagtcggaat ccaggttacg ttgagaaatc gcgacgagac ggcggaagat
     1081 cattccagct tgggagaggt gttatcggta gttagcacaa cgctcgcact tcgcaaggat
     1141 ttctggtagt tttttcgtac ctgagatttc ggggcgtgtc gtagcggccc acaagatcga
     1201 tgaattattt cgacgaaatc gccgatgtcg aagaccgggt gatcggaaga tcggccggaa
     1261 cgtggcctgt tcacggggtg gaggaactaa catgaagcgg ctcatcatct ttgttgcatc
     1321 ggtaattgca gcgagtgttt gcttgaccgc aaaagccccc gcggtaaggg ctcaatcctg
     1381 cgataccgcg cctgctccag actgcccata cggctactat gattctcctc cctacgattg
     1441 cgctccttac ggctactacg gcccggaatg gtttcccagc agcattttcg tgggcgccgg
     1501 tccgtggttt aacggccctg acaaattcca cggctttgtg gacaaccacc tcgatcctca
     1561 gcacggctac aagggcccga accccaaacg cggcgagaaa cctgacgcat cgaagcatcc
     1621 agataagaat cctgatttca aaggaaatga agtacgcgac gggcgcggcc acgtagtcga
     1681 agacaagcga tagcagccac ggaattgatc tacggtttgg gcggcacgct gatacgtttt
     1741 tgacaaccca tgtgtgcgcc ctcaggattt ggggatgacc tgctgacttt gcgaaggaga
     1801 aagtcatgca gttgcaaatg caactggcga atagaagttc gtcgtcatga tttcagcggt
     1861 gaggacaccc tggccagcaa caaggtcggc gcattccttc tttaagttcc gtgcgtgctc
     1921 gctcaatgtg ccgccttcgg gtttccggtt tcttgacgga gataacccag cagatccact
     1981 cattgcgtgc gagtgcagta atatcctccc attttgcccg cgcagccggg tccgaaatga
     2041 gaacttctcg taaatccgct ggtacactgt gcactgcgcc agcggagatc gcttttttgg
     2101 tcacacttta ttttacatcg ttccccagct agctggggaa ccgggcgcag tgacttttcc
     2161 cgtcggccac ctgccggtaa cccctgccag ctcaaccggt cgatgcaaca cctcataaga
     2221 aagtggcccg ctaacttact gaatcttcaa gggcacccaa agccactttt tccttccctg
     2281 tctgggcgga gcccaatcta ttacttctcg gaaactggtc ggttgagact tcaaagcact
     2341 gcaatcggag cgatctttcg cgaacgactg ctcgaagtgt agaatggaaa atttggggag
     2401 gtagtgaaat ggaattcctg gctggaagtc tgcttgatct gatcaccgag acgtccacga
     2461 atcttccgcc tgacgttcgc gccgcgatgt ccattgcggc caaagaagaa acgccgaaca
     2521 cgcaatccgc gcaggctctg ggcattattt tgtccaacat cgatatggcc gtcgacgaca
     2581 cgggccccat ttgccaggac acgggcatgc ccacgttcta tgtgcacacg cccgtcggcg
     2641 tgaatcagat tcgcgtcaag aaggcaattc tggaagcggt tgcggaagcc acgcgcctcg
     2701 gcaagctgcg acccaactcg gtcgactcgc tcacagggaa aaatagcggc aacaacctgg
     2761 gcgaagaaac gccagtcatt catttcgaac agtgggaaga agatgacatt gaggtgaaac
     2821 tgatcctgaa gggtggcggc tgcgagaata aaaatattca gtattccgtg ccctgcactc
     2881 tcgaccacat gggccgggca gatcgcaaca tcgagggcgt tcgcaaatgt atcctccatg
     2941 ccatttggca ggcccaagga caaggctgcg cgcccggcgc agtcggcgtc tgcattggca
     3001 gtgatcgcgc ccacggctac gaactcgcga agggacaatt gtttcgaacg ctggatgatg
     3061 taaatcccaa tcctacgctc gcgaagctcg aagcggaaat catggaagaa gcaaaccaac
     3121 tgggcgtcgg cgcgatgggc tttggcggga acgtttccct aatcggatgc aagatagtgg
     3181 cggctaatcg tctgccagcc agcttcttcg tttcggttgc ctacgattgc tgggcattcc
     3241 gccgcctggg cgtgcgcctc gatgcaaaga ccggcgcaat taacagctgg ttatatcgcg
     3301 atcccgaacg cgccgtgcaa cgcatggcag caagttcagg ctttcaatta agtggacacg
     3361 aagttgtcct gaccgcgccg ctgaccgaag cacagatgcg ttcgctgaaa gttggcgacc
     3421 tcgttctgat caacggtgag atgcgcaccg gtcgcgacaa cgtgcacgct tacctgatga
     3481 agaatcctcc gcccgttgat ttaaatggat ccgtgctcta tcactgcggt ccggtgatgc
     3541 tcaagcaggg cgacgattgg gttgtgaagg ccgcgggtcc gaccaccagt attcgcgaag
     3601 agccctatca ggctgacgtc atcaagaaat atggcgtgcg agccgtaatt ggcaaaggcg
     3661 gaatgggagc aaagacatcc gcggcgctaa aagaatttgg cgccgtatat ttgaacgcga
     3721 tcggcggcgc ggcgcagtat tacgcccgca ccgttgaaaa agtgttgggt gttgagctga
     3781 tggaattcgg cattcccgaa gcgatgtggc acttgcgagt aaaaaacttc gcggccatcg
     3841 tcacgatgga cgcgcatggc aatagcctgc acgcggatgt ggaaaaggct acgggagaag
     3901 ttttggaaac gttgcagaaa gtttgagaat ttagaaatgg tatgaatggg gcttcagtcg
     3961 agcgggtttg gaatccgcca aaccgaagcc ccatttttat ttatgttatt tatggatgaa
     4021 ttacgtagcg ggcgccaggt tcgtatcgag cgccaagccc agccgcgcgg ccagttcctg
     4081 tcgtgctctc cacaaacgcg ccttcagcgt gttgcgcgaa actccaagta catgcgcagc
     4141 ctcttccgct gacaatcctt cgatctctcg caaccaaaat gcggtccgca agagcggaga
     4201 tatttccgcc aatttctttt tcatgcgatc gcccagctca gtccccgcat aaagttgctc
     4261 tggattgggg ccatcgtccg caaatcggtc cgccagcggc aattccccgt ccagggcaaa
     4321 gtcgtccagc gaaaccgctg gcctggaacg cttgctacgc cgccgcataa gggccgcgtt
     4381 aatcacgatg cgcgtaagcc aggtggaaaa ctgagaccgg cgctcgaacc tcggcagatt
     4441 gcgataagct gaaagcaagc cctcctgcaa ggcttcctct gcatcttccg gattgcccaa
     4501 cacgcgaaaa gcggtctggt ataaggcgcg cgtgttgcgt gcgaacaagg cgtcaagcgc
     4561 cctcgcgtcg ccgttcagtc ccagctcgat cagttgatct tcagtctttt cgtccatgct
     4621 gatttcagta atattttccc ccgcggaatg atgtcgttcc cggttcatga tcgtttaccc
     4681 cttataaaca aggaatgcat ggagcctata agtgcttttg tttctccccc tacaactaag
     4741 agaccccaaa atattaaaaa ctctatgaat tagacgtcgg cggaagcatt tggtggccta
     4801 ttcccttatc tttcttgtgc tctgccggtc gcatcctgca aactcagcgc tcctgcgcaa
     4861 tacgaaaggt aaattcagtt cgaatttagg gaccccgaaa tttcttcgac tacagccctg
     4921 gtcatgcagc cacaacctac gattccaatc cctgcctcat gcatctgtgt tagtaattgc
     4981 tttcctgccg catcaatgta gctgatcgct ttcagacgaa tttgcagagg gacattcaaa
     5041 ggcgattgcc gttgtttcca gcaccgctcg agttccgtaa cccattcgcc ggagagtttt
     5101 ccctccagtt cccaagtctc tgtgttatcg gcctgactta cggtaacctt aaacatttcc
     5161 agccctgtgg attcagctcc aagaaagcat cccgatctat accgtagcat gttattggcc
     5221 attgtgccga aatagaatca caagaaataa ctgtaatatt tacagtgcaa tacgtggata
     5281 atttttcgat tggattagaa attcgtagtg aaagagggtg taaatattgc cgctatgtcg
     5341 gcaggtgccg atatagcggc atgacacttc atttctgttt ctctatccct aattttcgca
     5401 tttttgattg taaggttgtt cttttcatac ctaacctcac ggcggctccg cgggggcctg
     5461 ccaataccca tttcgttgtc tctaaagctt ttagaatgtg atcgcgttcc gctgctctga
     5521 gggtctggct ttcaacaagg gcttcaccgc cggatttaag ctcactcaaa ggtacgcgaa
     5581 gcactggtcc cggggacacg ataaccgcgc gttcaatcaa gttttctagt tcgcgaatgt
     5641 tccctggcca tggatagcga acgagctgtt ctaaagtttc ggccggaatg gtctccactt
     5701 ttcgattcat gcgccgggcg tgtttttcta caaaatatcg aaccaacgag gaaatatctt
     5761 cctgcctgtc gcgcaaaggc ggcataagta ttgggaagac acgcaacctg taatacagat
     5821 cgcttcggaa agtgcgcgcc gcaaccattt ccgccagatt gcggtttgta gcggctacca
     5881 ttcggacatc gacgtgcgtt gtgtgcgtgc tgcctaagcg ctcgaattcc tgttcctgaa
     5941 gcacgcgcag aagctttggc tgcagttcga gcgggatgtc tccaatttca tccagaaaaa
     6001 tcgtgccgcc atcggcgagt tcaaacctgc cgattcgctg agcgattgct cccgtgaacg
     6061 ccccgcgctc gtgcccaaac aattcggatt caagaagacc cgtgggaatg gccgcgcaat
     6121 tcacttttac aaaggtacgt tcccttcgcg ggctcaaatt atgaatggcc cgcgcgatta
     6181 attccttgcc cgtgccggtt tcgcctgtaa tcagaacggt ggaatccgtg ggagccaccg
     6241 tctccacttg cttgagaacg gcgcgcagcg aggcggattc accaatgatt tcatcgaaat
     6301 tcatttcaga acgaatttca tcttccaggt agagcttttc ctgcgccaat ttatttttga
     6361 gttcggcaat ttcctggaag gccagcgcgt tttcgacggc aatcgccacc tgacttgcga
     6421 cctgcgcaag caaatccacg tcgccttgct ggaatgccga gtcgcgcagg ctagccaggc
     6481 tcagcgtgcc gagcgcgcga tcatgtgtga tcagcggcat gcacaccagc gatcgaaccc
     6541 cttcgaccat cattagccgt gaggtaaccg aatcgaacgc ctcgatttca ttgcggtcca
     6601 aaacctgagc atgatgggtg cgaaaagcct ttcccgccag ggccctttct accgggacaa
     6661 gcaggtcctc acgaatcagc cctttaccct caggaaagtt cagagccaat acacgcatgc
     6721 tgtcggtttc cggttcgaaa accgcgaggc tcgtatattc gtggtgaatc acgcggcgga
     6781 gcgatgccgc aatcgcattc aaaagcacat gcaggtccag cttggagacc accgcgttgt
     6841 ttacttcaag caggacgcga agcctgtcgc gctccctggc cagttgccgt tgataagcct
     6901 gagcgctttc aaaattgagc gcgttgtcca cagcgacggc tacctgccgc gccacctgct
     6961 gcagaaacgc gacttccgat tccgtgtgaa tatgctgcgt accgcgtccg aaactcatga
     7021 cacccaggcg acgctgcgca gtagtcagtg ccagcgagca aaacgaccgc acgccatttt
     7081 cgcggagagt ctgcacgatg tcgggaatcc gcgtttcctt ttcgatgtca tcgagaagat
     7141 atggcttctg cgattcccaa acccagccgg agggcgtctc tccaacggaa aactcactcc
     7201 ccgggccata tttcctgaat tgtttttcgg attccagaat gtgcaagcgc atgacgttgc
     7261 gtagggggtc gtacaagata aggttcaggt attcaaattc cacgacgcgg tgcaggcgtt
     7321 ccgcaagttc gtgaaacagt tcgccgagat cgcggtgttg tgcgatcgct tcagccacct
     7381 cgagcagggc ctggtggcgt gcttccggag aagtgctgcc tgcaacggga atgggcactt
     7441 cgctcattgt tcggagaata acacctactt ctattatctc tcaactctag cgcgagaatt
     7501 cgtgaagtct tcgcgcattg caaaagtttg cggaacgcgg atcgagcgat gagccgcgac
     7561 aaattttaat cgaaggaaaa tgtttcgaaa agttacaaaa aggaattgac agctgccgga
     7621 atgtacgcgc ataattatgc aagtgggtgc ataaagttaa gtcgaccatg gcgctgaacg
     7681 tacacaaaaa attccggcaa gggcaaatcc tgaaactggt ctcgagcgaa cctatctcga
     7741 gccaggacga actgcggcgc agactttcgc acatgaaagt gcgcgtcacg cagggcactc
     7801 tttcacggga catgtccgaa ctgaaattgg tgaagaccgc cgacggttac aagccgctta
     7861 cgaacggcga cgaatttgcc gctccgatgc cgccgctcgg ccgcgcgctc agggaatttc
     7921 tggtcgacct gaggcctgct ttcaatttac ttgtgttgaa gactccgccg tccggagcgc
     7981 agccactggc tgccgcactc gacgctgaaa aatttaagga agtcgccggc acaattgccg
     8041 gggacgatac cgttctggtc atcacgccgt cgcgccaggc gcgcgccgct gtccagaagc
     8101 gcatggagga tttactccga tgaacgaccc gcaacgggtc gccgtagtcg gagcaacggg
     8161 ctatgcaggt tttgagctgg cccagcttct tcttcggcat cccaaaattc aaaaaccgac
     8221 cttttacttg cgggaaagtt cggcacatgt aaagtgcctg accgaaattt ttccgcacct
     8281 gcgcggctgg ggcgaagctc cctgcaagac gttttcagtc gatgccattg catcgagcgg
     8341 agccagcacc gtgtttctcg cgacgcctca cgaggtttcg ctcgaaactg tgccgcaact
     8401 gttgaacgca ggtttgcggg tagtggacct cagcggcgcg ttccgcttcc acaatccgga
     8461 aacgttttcg cagtggtaca agcttccggc gccgccaaga aatttggcgg aagaggctgt
     8521 ttacggcttg ccggagttgt atgcggatca attgcccggt gcgcgacttg ttgcgaatcc
     8581 cggctgctat ccgacaagcg taattctcgg cttgcggcca ctcgtcgaat ccggctggat
     8641 cgcaacggag cgcggaatcg tggccgattg caagtccgga gcgaccggcg cgggtaagga
     8701 accgaagaag gaaatgcaat acgtggaagt agacgaaaat tttcgcgcct acggcctgtt
     8761 ctctcaccgg cacacgccgg aagtaaccga gcacacgggg atcccgggcg aggacatcgt
     8821 tttcacgacg catcttctgc ccgtctcgcg cggcattctt tcgactcttt atgtgtggtt
     8881 ttccgagccg catacggcgc aggaagtgga atctctctat cgcgcttttt atgcggatcg
     8941 cccgatggtg cgcatctggc ctgcgggaaa attgccggaa ctgcagcatg tggcacacac
     9001 aaattttagt gacatcggtt ttgcactgga tcccgcgggg cggcgcctcg cggttgtgag
     9061 ttgtctcgat aatctgggca agggtgccgc gggccaggcc gtacagaata tgaatttgat
     9121 gatggggatt ggggaggcaa caggactccg atgagactag tcgtaaaagt tgcgggagcg
     9181 ttactcgatg atgcggcggc gatggaaacg ttggcgcgtc aggtgtccca gcttgctcac
     9241 cagggccacg aaattctcgt ggtgcatggc ggaggaaaaa tttttaccgc caccctcaag
     9301 cagatgggaa ttgaaagcaa gttcgtcagt ggactgcgcg tgacggatcg cgcgacgcgc
     9361 gacgttgccg tgatggttct cggcggtctg ttgaacaagc gcctcgccgg cgctatatcc
     9421 gctgccgggc agcctgccgt tggtatttgc gcgtccgatg ccagctgctt tttggctgag
     9481 cccatgatgc acgacgaagt ggttggcgga ctgggcttcg ttggctatct aactggagtg
     9541 aatctggaat tcctggaatc gttttggaag gccgggatta ttcctgtcgc gtcttgcatg
     9601 ggcttgggcg ctgacggtga actctacaat atcaacgccg accacatggc agcggcatgc
     9661 gccgaatata ttcacgccga ccggctaatt tatctcaccg atgtggctgg cgtcctcgat
     9721 ggctccaaag ttgtgaaagt ggtttcgact ggcgatgcgg aagacatgat tcggcagaat
     9781 aaagtttcgg gcggaatgat cctgaagctc gaagcctgca aacgcgcact ttcggccggc
     9841 gtttcggaag tgagaatcgt tggcggttcg gtgccaaagg gattgctggc tgcggccaac
     9901 ggggcgaggt ttcccggaac acgaatcacg gccgagtttc ccgtgacgag cgctggagcg
     9961 gcccagtgaa accgaaagca aaaaaatcgg caacgaaggt gaagccagct tcggccgcaa
    10021 caaaatccat cttgaacgat gcgtctcagc atctgatgaa cacttacagg cgctcggaga
    10081 tgatttttac acacgggcgc ggctgctacg ttttcgatca aaacggaaat aagtatctgg
    10141 attttctcgg tggcatcgct gtcaatgcac tcggatattc ccatccggcg ctggtgagga
    10201 cgattcggcg cgaggccggg cgagctgttc acgtttccaa tctttttcat aatcctttcc
    10261 agggaccgct cgccgcgaaa ctggcgaagt ggtccgggat ggaccgcgtt ttcttcacca
    10321 atagcggcgc ggaagccgtg gaaggcgcgc taaagcttgc gcgtatcgcg gcaaagcaaa
    10381 agccaggagg cgcaaccaag actcgctttc tggccatgga acattcattt cacggtcgca
    10441 cgttcggctc gctttcgatt actcatccgg tgaaataccg cgagcccttc gagccgctga
    10501 ttccgggagt agaatttgtt cgcttgaacg atgtggcgga ccttgaatcc aagtttacgg
    10561 acgacgtgtg cgccgtaatc gttgagccta ttcagggtga aggcggagtt tacccggtca
    10621 caaacgaatt ctggaagcgg gcgcgggcgc tcgcgacgct gcatggcgcc gtgctgatcg
    10681 ctgatgaaat tcaatgcggc ctcggacgca cagggaaggc atttgcctac caaggactcg
    10741 aaggtttgcc cgacattgtc atcgtcgcaa aacctcttgc cggcggactg ccgctcggag
    10801 cgcttctggc gcgggaagaa gttgccgcat ctttttcgcc tggccttcac ggctccacgt
    10861 ttggtggcgg accgctgacg tgtgccgtgg gcctcacttt tctcgagacg gttgagaaaa
    10921 agcatttact cgccaatgtc cagaaacgcg gcacggagtt aaaagccggc ctgaaaaaac
    10981 tggcagccaa atttgatttc atccgcgaaa tccgtggcga aggtttaatt atcggtatcg
    11041 acctggatgt ggacggcgcg ccatatgttg cggaagcgct gaaacaaggg cttctcatta
    11101 attgcacgca cgaccatatc ctgcgcctgc tgccaccgtt tattttgact gcgcaacagg
    11161 tgaaggaagg tttatcgaaa attgaatcgg tcctagccaa gaccgaacgg cccgcaccgt
    11221 cagcggccgt aacctccacc ccaaccgaag ctcagatgct tgcggccgtg aggtaaacat
    11281 gaatgcgcag gccccggctc agggacaaac cgagagccac acattgagtc tttccaccga
    11341 tcttctcacc ggcacggaat ggaatgccgc gcaggttcag gaacttttcc gcctgtccgt
    11401 ggacgtcaag gctcatccgg atcgttataa gtccgctctg gcgggacgct ttctcgcgat
    11461 gatttttgaa aagccgtcgc tgcgcacgcg cgtaacattt gaagtgggca tcaccagcct
    11521 gggcggcacg gcgatttatc tcgatcatgc cgacactcac ctcggcgatc gcgaatccgt
    11581 tcccgatgtg gcgcgttgcc tttcgcgctg ggtgcacgga attgtcgggc gcgttttttc
    11641 gcaggatgtg ctgcagattc ttgcggacta tgcaagcgtt ccggtgatca acgctctctc
    11701 tgacgtctat catccttgcc aggcgctgtg cgattttttc acgatcgaag aaaaattcgg
    11761 ctccgcgcgg ggcgtgacgc tcgcctacat cggtgatggc aataatgtct gccactcgct
    11821 gatggttgcc ggcgcacgag tcggcgcgca tgtgagaatc gcgacgccag cgggttacga
    11881 gccggacgca aagatccttg cgtcggcgcg tgtcgacgcc gctgccacgc aaggcacaat
    11941 tgaattattt cgcgctcccg aagaagctgt cacgggcgcg caagctgtgt acacagacgt
    12001 ttgggccagc atgggacaag aagaggaaag tgaaaaacgt gccgctattt tcgcgcccta
    12061 tcaggttaac gccaaactca tgtctcaagc tgcgtctgac gccgtgtttc tccactgtct
    12121 tcccgcgcat cgcggctccg aagtaacgga cgacgtcatg gaatcgccgc gatccatcgt
    12181 ttttgacgag gccgaaaatc gtctgcacgt tcagaaagcc atcctcttaa tgctcctaag
    12241 ctgaaaggac aaccggtgcc aaagaaagta gttctcgcct attcaggcgg acttgatacg
    12301 tcaattatta ttccgtggct caaggaaacc tacggcggct gcgaagtaat cgccatgatt
    12361 ggtgacgtcg gccagcagga agatttgaaa gcagcgaaga aaaaggcgct ggcgacgggc
    12421 gcggcttcgg caacgatcga agacttgcgc gaagaatttt tgaccgagta tgtttggccc
    12481 acgctgcgcg caggcgcggt gtacgaacac aaatacttgc tcggcacgtc catggcgcgg
    12541 cctgtaattg ccaagcgcca ggcggaaatc gctctcgaaa cgggagctga cgccgtcgcg
    12601 catggttgca ccggcaaggg caacgaccag gtgcgcttcg agcttgcgta caaagctatt
    12661 gcgccgaaag taaaaattat ttcgccgtgg cgcgaatgga ccatcaattc gcgcgaggat
    12721 gccatcgcct atgcgaatgc acacaaaatc cccgtcgaac agactaaggc caacatttat
    12781 tcgcgcgacc gcaacatttg gcacttgagc cacgaaggtg gcgtgctcga agatccagcg
    12841 aacgagccgg acgaagccat gtggcaatgg atcgcctctc ccgaaaaagc gccaaacaag
    12901 cccgcgctcg tcgagatcgg cttcgacggc ggaacgccag tttccgtaaa cggcaagaag
    12961 cgttcggcca tggtgctgct caatgagttg aacgaaattg ccgcggagca tggagtgggc
    13021 cgcatcgatc tcgtcgagaa tcgcttggtc ggcatcaagt cgcgcggcgc gtacgaaacg
    13081 ccgggcggca cgctgcttat caccgcgcat cgcgaactcg aaacgttgtg cctcgaccgc
    13141 gagaccgcgc attattcgga aatactgtcg ctacgctatg cggagcttgt gtactacggc
    13201 acgtggttca cgccgcagcg cgaagcgctc gatgcttatt ttaccgaggc tcaaaagcgc
    13261 gtgactggca cagttggcct caagctgttc aagggcaacg tgaccgtcac aaaacgcgtg
    13321 tccccgtatt cgctctatcg caagggcctc gcgagtttcg ccatgacggg ctacaacccg
    13381 aaagatgcgg aaggattcat taacttgttt gcattgccgg taacgattcc ggcggaatcg
    13441 aaagtaaagt gaccgctacg ccaaaagagc agccgtcggg aaaattgtgg ggagggcgtt
    13501 ttgaccgcgc ccctgacgaa ctgttctacc agtttcaacg atccttctca ttcgatcggc
    13561 gcctgctgcc ctacgagctg gcggtcgatc gggtgtgggc gggcgcgctg gaatcggcgg
    13621 gcatactttc agtcgaagaa atgcgcgaca cgctgaccgc gctcgacaaa attgccggcc
    13681 gtgcggaaaa cgaatatgcg tggcttggcg gatcacccgc cgaggacgtg caccattttg
    13741 tcgagaccgc tctcgttgaa tatctcggcg ctcttggata caagctacac acgggccgca
    13801 gccgcaatga acttgtagcc actgatttcc gcatgttcgt catggaatcc gtgggcgaaa
    13861 tgcgtgtagc tctgtcccgg ctgatttcag cgctcgtcgg acaggcggaa aaagttttgg
    13921 gaattcctat gcccggcatg acgcacatgc agcacgcgca gccgattttg ttttcgcatt
    13981 ttctactggc gcatgcggaa gctttttttc gcgacgcggc tcggctcgac gcagcggaag
    14041 ccatggcgga cgcttgcccc atgggttcag gcgcgcttgc aggctgtgcg cttgatgtgg
    14101 atcgcggagc tatcgcccgc gaactgggtt ttgctcggcc cacagccaac agtctcgatg
    14161 cggcgagcga tcgcgatttt gcgcttgatt atctttacgc gctggccgtc atcgccacgc
    14221 atctctcgcg cctcgcagaa gatttcgttt tatttgcgtc gcaggagttt gggttcatcg
    14281 ttttatctga cgaattttca acgggcagca gcctgatgcc gcaaaagaaa aatcccgatg
    14341 catgggaact tctgcgcggc aagacggggc gcgttactgc ctcactgcat actctgctga
    14401 cgactttgaa aggcctgcca tcgagctatc agcgcgattt gcaggaagac aaagagcctg
    14461 tattcgcagc tcacgatcag gcatatgcca tgctgaccat cgctgcggga gccgtggccg
    14521 ctacgcgtcc gaacgaggca cgcatgcgcg aagccgcgtc cgatcctgcg ttgctcgcta
    14581 ccgaggccgc gcattatctg gtccgccgcg gcgcgccgtt ccgccaggcg catgaaattg
    14641 ttggccaaat cgttcgcgag ggcgagcgcg ccggaaaatc gttttccacg ctgccactgg
    14701 aaatactgca aaagttttct ccattgtttg cgccggattt ttacgacgcg ttgaaactgg
    14761 aatccgctct ggctggcccg gacgtcgaag gcgggaccgc gcctgcgcgt gttcgtgacg
    14821 ccatcgcagc agcaaaagcg cggctggctg ctctggagaa aaaatcatga gggcgcgcac
    14881 agctcgcatg agtgacgcgg ccgcggttca ccgcgtcatc gctcactatg ccggcgaagg
    14941 ccttcttctt ccccgcacgg aagcggaaat ccgcgaacat atcgcgcgat ttattgtgct
    15001 ggtggaaaaa cgcgggaggg aagaaaaagt tctgggctgc gtagccctgg aaccttatgg
    15061 cgcggatctc gcggaaatcc gctcgcttgc agtcgctccc gaggctcgtg gagaaggccg
    15121 caatgtcggc gagcgtctga tgaaagccgc gatggacact gcgcggcgaa gaaaaatcgc
    15181 gcgcctgttt gccgtaacgc acaggcctga atttttctcg cgctacggct tcacgcccgg
    15241 tccgcggcag attgtgcccg aaaaaatcga gcgcgattgc gccacgtgcc cgaaagaacg
    15301 cacctgcaat ttgatcgcga cggttgcggt ggtttgtccc gaacgcgatt tgctgcccgt
    15361 agtgagtgga gcaggaaaag ttctttcaat gttgtaatag tttttgtagc aaacttttgt
    15421 agctcgcctc tcttgtcctg agtgaaaacg aaggatcaga ggcgagggtt taaatctgac
    15481 gtcacctgtt cttagtgtgt gacatctcga cccccgccaa taaaaacgcg ggctacagca
    15541 caacttgaat tttaaattgg tgatcaaaaa atgagcgact tgccttcatc ccgcgccttg
    15601 ccgcgcgggt ttcgttttgc atccgcctcc tgtggcctta agaaacgcgg ccataccggt
    15661 acgctggacg ttgcgatgat cttttccgat tctccagcgt ccgcggctgc cgtgtttaca
    15721 caaaatcttg tccaggcgcc acccgtggtt ctttcccgcg agcacgtgcg cacgtccgcg
    15781 gactcgatgc gtgccgtcat cgtaaattcc gggaatgcca actgctcggt aggcccgggc
    15841 ggcatggtcg cttcgcgcgc cactgccgaa accgtgggaa aactcctcgc ctgccgcccc
    15901 gatcaaatcg ttgtgtgctc gacaggagtg atcggcgtgc ctttgcaggt ggagcgtatc
    15961 ttgaatgccg cgccaggcct tgcgaaatcg ctggaattat ctgcgcgcgc tttcgatggc
    16021 ctgaatcgcg cgattatgac caccgacacg cgcccgaagc gcgccgccgc aagctgcaaa
    16081 atcgccggta aaacggtgcg ggtcgcaggc tgcgccaaag gcgcgggcat gattcatcca
    16141 aatatggcaa cgatgctttc attcgtcgtc accgatgccg ccgttgcgcc agcagttctg
    16201 gatagtgcat tgcgcgaggt cgtcaatcgc acattcaatt gcatcaccgt ggatggagac
    16261 agctccacga atgacacgtt acttgtgctc gccaatggag cgagcggcgc gaaagaaatt
    16321 acaaaaacag gcggcgcaga ttacagaata tttgtcggcg ccttggaaaa agtttgcaaa
    16381 aatcttgcga ttggaattgt ggaagacggc gaaggcgcaa cgcatcttgt agatatcgaa
    16441 gtgcgcagcg cacccagcga tgccgccgca aaaaaagttg cggagaccat tgccctttcc
    16501 gcgctggtaa aaacggcaat cgccggagcc gatcccaact ggggccgcat tctagccgct
    16561 gccgggcgcg caggtgtgcc attcaatcca gagaaagcag aaatttggct gggaaaaatg
    16621 aaaatgtacg gtccgccaaa gggctcgtcc tactcggtcg cgttgcccct caatgagcgc
    16681 gcggcgcata aacgcctgct gggaaaaaat gtttccatcc tcatcgatct acacaatgga
    16741 cgcggcgcag cgcgcatgtg gacctgcgat ttcactaaag attacgtgga aatcaacgcc
    16801 agctaccgga cgtagtttcg agtagcgcgg gcttgaacct tgcaaggcct aaaccccgca
    16861 ggctgaagcc cgcgccacta aaatcgtgca ccgaaaaatc gaaaaggaca aaaaacaaag
    16921 atttaatctg ttttttttgc tggataaatg aaaataaaaa ttatggcggt gatacccact
    16981 accacgaatg acattacact gccctcgggg cctaccaaac cgcccgtgag ccacgcagga
    17041 ccgtgcaggg aagaatttag aaaatggccg ggcgcgacga gaccgctgtc cggcacggaa
    17101 aacaagaatg tttcgcccca gtcgaaggca gcgtgcagcc ccacggcgaa ccatagattt
    17161 ccggtacgcc atagcgtcag gcaaaaaaac attccgatca caaatacgct cagtgcgccg
    17221 attttgtctt cgcctgtatt gtgcaggtgt atcgcgccaa agcctgccga gagcaaaatg
    17281 gcggcaggcc agaatccaag acccatggag agcgtacatt gcgcgtaacc gcgaaataaa
    17341 aattcctcaa aaattcccac caaaataaaa actacacccc acaggatcgc atagcctaag
    17401 agatgcgagc catgcaatgc caactcgcca aatgtaaatc cgccgaacgc gcgaatcagg
    17461 aaaatgatcg ctgtgatcat cgcgattccc cacaccgtcc cttgccaaaa tcgtgagcca
    17521 aaggcctcgc tgaacggcag gccgtaggtt ccaaatcgcc tgtcctccag catggacatg
    17581 atgcccgcgg ccatgaacgt cgcgacgatt ccggttactt cctgaatcag catggaagtt
    17641 gccgtgacgc ctgaacggga aatttccggc agatgcaaaa cggtgattaa aaaagtccca
    17701 accagaccga atatgacaac gaaaaataaa tacatcagga gacgccagcc ggcgcgaaat
    17761 tcggtctcgt tccagaagat ggcttttctg ccggtgcgcg gccccgcggg cggtcccaga
    17821 ggagattgcg acgcggggat ttcagaattt tgttcgggca tcatgaagtt actccggacc
    17881 atgttcagcg taacgcttaa gtcgcgcgag ataattttcg ttgcggcgcg cgaccgcatg
    17941 gcgcgtcatg agccagtcca tcatcttgcc gagaaagccg ggcatcgtgt agtcgctgat
    18001 cattcgcacc accgttccca gcgaattgtg agctgaaggc actgtcaatg attccagttc
    18061 ccatcgctcc gaacctttga cgccgtagcg gtcgctgaat cgccactcaa acaatcgagc
    18121 ctgctgcaag gcggtgacga cggcggtgtg cgacacttcg cgttttccta aatttcccgc
    18181 gatgtgcacc ttttgcgcaa cggcgaaatc tggcgcaccg ccctgcactt caaggcatgt
    18241 ctgcatatcc gcgccatacc agtatggcat ccgttgtgga acgaaaaacg cgaacaccgc
    18301 ttcggcggga gccgcaatct gtaccgaggt ttcaatccgc gccatggaat gcgcgctact
    18361 ttagcacggg cgggtgccgg aaaataatgt ggcatagact tcagcctgtg gggttaagtt
    18421 ttgcgaggac caaaccccac aggctgaagc ctatgccacg aattccactc aaactttgga
    18481 gaagggaatt gccgcgactg cgagaatatg tcggctatgt cgctcctgca caaggaccac
    18541 ggcacgcaaa ttatcgcgat tccattcgtg tccgggatgc aaagttgtgg tcgaggaaaa
    18601 tcctgaggac ggagaattaa tttttgcgac gagcgaaagc tgccggacga catcgttgtg
    18661 ttcgagggcg cggccggcat tttctccgcg cttcacatcc gtgtgcagca tgttttccgc
    18721 gaccgccaca agcagttgca gatcgtctcc gtgcatggcg accgtgagcg ggctaacgtt
    18781 tattttcaga ttgtcgcccg tatcccagga gagttgcaca gcgggtttgg gcgactggct
    18841 cgccgagcgg atggcgcgca gcgcatcatt atccgaaccg ccgacaaatt ccgcgcgtcc
    18901 atcgacaatc atttgcggcg tataaatttg ctgtccaccg aaggcagagg catagtcatt
    18961 ctggcgcacg gtcgctgctt cggatgaaaa cgggtctttc cagccgaggc gatcccaata
    19021 atccacgtgt tcttccaagg cgatgatgtc cgcgccgtga accggctgcg ttcgaccgag
    19081 gcgcgaaaga agagcatccg caggagggca actggaacat ccttcggagg taaacagttc
    19141 gaccaaaacg ggcgtcgcgg ccaggtccgg cgcaactgcc gcggaaggat tattttccaa
    19201 atccgccctc acggaatcgg caaattcggt ccgcctcagc gccacgaaac caagcagcat
    19261 caacgccgcc atgagaacag taactctaac cggttgaagg ctcatcggat ttctccctcg
    19321 cccttcgttc gaaattcatc ccttcgcaaa agaatacgaa gttcgagggc gggaagttac
    19381 ggccgctcta gtgcgcgcgg ccgtagccgc cgaagtagta acgcgctccg atcgtcgcat
    19441 aaagtccata gggtccggaa gttccgagat ccaggccagg gactgccaga tcaatattct
    19501 tgttccggta cttgccgagc caatccacct gatgctgcgt gatgcgaatc tcaaaattct
    19561 tcggcagttc gaagccgacg cccacggagc gttcaaaggc aatgcccgac atcgacgctg
    19621 tatatttaaa ttgtggcaca ttgctgccca aggaaaacgt aggcgtgaag aacacgaata
    19681 aatggcgcgc aagtgttttc ccaaacggct gaatttcgag atagccgccg agcaagtagc
    19741 gtgcataggc cgtgcatggc gaattggctc cgccggcggt ggcgatcaac gcccccgact
    19801 gcgtgcagcg gccaaggtcc ggctcgttgt gcgatggggc gacctcgaag tctgcatagc
    19861 cgcgtatcga attgtgcggg aaaaacattc ttccctggga cgatccccac gatgccggtt
    19921 tcggcgcggg atccgcttcc gaactggcag cggagctcgt ggtggaaagc gtccccattg
    19981 cgtcctgggc caagaccggc aaaccagtaa atacaaaaac agctgacagc aacattaatt
    20041 cgcgaattcg gtttctcacg cgattctcct ttaactttcg gaaaaatata aaccggttca
    20101 cttatttgga tggactcgca acatcgcccg tgcccttcac aaattcgggc gcgcggccct
    20161 gggaccacgg atcgtaagtt tgcgcgaaac gcatgcgctc atcgagcggc ggatgattgt
    20221 aaaaccagag tttcacggcg gtcgaaggac tgggctcctc cagatcgact tcgcccagaa
    20281 tttgaaacgc gtgcgcggcc accacaggcg catccggaat caagccgtga ataacttcga
    20341 gtccatactg atcagcctga tgttcgagat ggcggctgta agcgtttgag atgggcgtaa
    20401 acagaaatcc aaaaacggat aagagaaaaa gtaaaaccgg caaggcggcg agatcatccg
    20461 gcccgcgaat agcccaggaa tttccccatc gtgcgagcat ccagttcagc gaacggtagg
    20521 aaagattgag gaagaacagg agcacggcgc aggcaaaaag aattccgtcg cgcacgtggc
    20581 ccagaacgta gtgccccatt tcgtggccgg tcacgaacaa cacttcgtcc tcgctcatgt
    20641 gccgtatcgt cgtgtcccaa acgaccacgc gcttactcgc gcccaggccc gatacatagg
    20701 cattcagttg gcgtagcttg gcgctggccg tcatctccag aatgcggctt tgtggaatcg
    20761 gagcgccggc gcgaatggtc atctgctcga tttttgtggc gagtcgcggc tgcgcatttg
    20821 cgagcggcgc aaatttgtaa tacagcggct cgataatcac gggctcaacg attgcgccaa
    20881 aaataatcag cggcaccgct gcaatccaaa accagaacca ccacttccgc gggctcttgc
    20941 gaatcaccgt aaacaagatc cagaccatga ggctggcaat ggcaatttca acgccttcgc
    21001 ctttggccca atccgaaagc cacgaattcc atccctgaat ggattgctgg tattgcagcg
    21061 ccaggcgatg cgcccaaatc ccgataggca ggctcaagac atcgattgta atgaacagaa
    21121 gcggtgcaaa aacgacgatc tggccgaaat cgctgtccgt ccattgtgtg gcccaatcgc
    21181 ggtacctggg agcgacgcga agctgcagaa tcatcacgag cagcaggagc ccataggcgg
    21241 catccagaaa ataaagttca tgccgggcat gcgcatacgc gatcgctttt gcttcctgat
    21301 ctggagttag cgtgtagcct tgtacctgcg gcgacgaagg ttgctcaggg gaggtagtgg
    21361 atggcgttgc gggcggttgc gtgtcttgcg cggagccgga cgccgcaaag aaaaaacccg
    21421 ccgctacgaa acttaggaaa acgaaaaaaa actgtgcgat ttggcggctt ctgcgattca
    21481 cgtgtcttcc tccggccatg cccagcactg accgttccgc gccggtaaga aagcgggcac
    21541 ggcgcggagt atgatctaac ctctgtcgcg ggtgcaagca tggtatcgct tgcgctgccg
    21601 tcaatcatgt aaaaatttgc cgcgacttaa tttggcgatt tcagattgta gttgcgaatc
    21661 gcgccgccca ccgcaacacc cgcgtgaatg gcggaagtcc accgctccag acggtgatgc
    21721 ccggtgcgat gaaacacgta ggaaactccg accgaagccg ccgttgccgc gatctcgatc
    21781 cccgcaaata aaggacgatt gtcgacaatg gaattggtaa ggaacgcttc gttgatgccg
    21841 cggtggcgaa ggttcagcgt tgacgcgtag tccagagcgc gcccggcgcc cacagctgca
    21901 aacagcaaat cattctcgcg atcccaaaac ttatgtactc ccggcgccac tggattcagg
    21961 gtcggggcaa ttccgccgtt tgtcttctga tcgggcttgt ccggtttatc gggcaaattt
    22021 gcgttggccg caggttttgg ggcgtccggc gcgctcggat cccggccttc gcccggttgc
    22081 tgctgcgcga atcccgcgga aacgctcgcg agcgatagaa acgcggcggc aatcgcagtc
    22141 aaaaaccact tacgcatcga aggtatttat tcctttcatt tcactcaatg gaaagaaaga
    22201 ttgtgggcaa gcacggaaag gttgtaaagg aagaaagaat aatttcgcgg ccgctttttt
    22261 gggcctggga gcggggctca gcggtgcgct taaaaccgtt ttgcaaccgt accactagga
    22321 attgtatatt ttctcttgac accacaagag caggagcgca aaatcccggc aacttttccg
    22381 attctttccg gtctggggaa ctgaattttg gttgtgactg ggaaaaaatc gtgcatcgta
    22441 ccacaggggg gaaagcgtgc agtatttgcc tggatggacc gtgcaatgta ttaatccgga
    22501 gtgcgcggca cgcggccact ggctgcgagc tgccgatgca gggggagaat tgtgcagcaa
    22561 ttgcagcgct ccgctccgca atgtcccgcc tccgctgggc ccacgcttgc ggatgcgtcc
    22621 acgttcgttg gggagttacc gtccgaaccg ttcacccggc cggcaacggt agtctccgcg
    22681 agcgatctca gggccgctcg tcccggtttc gccgggacga gcagtccccg ccattttttc
    22741 cctaagtaaa tttaaagtta ggacccgtgg cataggcttc agcctgtggg ggtgagtttt
    22801 gcgaggacca aacccccaca ggctaaaacc catgccacat aataccactt taattgggtt
    22861 gcgaagatgg ggagagcggc cgaatatccg gccgctcgca gggcatgggc aaactcaaaa
    22921 tcgctgcagt tgtgcaaact ctatcgggaa tgactcgccg gccccaaaaa actcaagcag
    22981 accagcaaag caaataccgt gatgcatgtg acggctatca tcggcgctcc cggccggtcc
    23041 gaattgctcc gacctgagat cattctgcgc gaaccgtaat tttcaggaaa tagaacggga
    23101 gttacattcg caccggtact ctcgtacgtt acttccgatt ttcacacgtc ttttcacacg
    23161 cccagatcag gaattaattc tgtcgcgtcg ttgaagcgat tcgtaaaatt cgccatgcag
    23221 atcaccagcg tcagctccac aatttggcct tcgtcataat actttcgcaa accctcaaga
    23281 gccggtgagc gaattccgga agcgccgcgc gtaacgcgat cggcataaag aatcgtggcc
    23341 ttttcctttt cgtcaaacag cgggctgcgt gcgtaaaatg ggagcgcggc aatttgttcc
    23401 ggagtgacac ccgcgccttt tgatgaagcg atatgcgcgc gtgtgcaata ttcgcagccg
    23461 ttgaccatcg aggcgcgaag gtaggccagc tccttaaact tgggctcgac ggttccctgg
    23521 tttacgacgg ccttgtagag gggaagaaat gtgctaagga cagcaggccg gtgggccatc
    23581 gcagcaaaaa tattcggtac tttcccggca cgcccggcca gcttttcaaa tgtgtctttt
    23641 aattccggcg cggcctgttc ttttgtcagc ggattcacga ctgccatgat cgatctcctt
    23701 ttcgttactg gattgcggat tgagatttat gtttcccggc gcaagtttaa caagagcggg
    23761 aaaattcggg aatggggagt cctgcaaatt ttaaatgctg tgtgattgca gcttgcaagg
    23821 acgaaaaccc cgaacgctga cgcccgcgcc actggtaacg ttgtggcatt gaatggcgtt
    23881 tcagctcgtg ctagcctgct cggagtaaag accttccagc ccatacattg gagtaaggaa
    23941 aaccttctga tggtgtcttc cgcacgccaa attaatgcag aggccgatca agaacgcata
    24001 ttgagccttg tgcgggagct tgtcgccgag cttggtcacg aatcgggcgt gcgttccact
    24061 ggggttcact ctcatctcga tcgcgatctg ggtcttggca gtctggagcg cgtggaattg
    24121 ctggtaaggt tggagaaaac gtttggcaca cggctgagcg aagacgtcgt ggccggagct
    24181 gaaattgttc aggacttgat cgatgcggtg agcactgcaa atggcgaacc tgtcaatgcg
    24241 cgcttacagg atcagaatgt ttcgcgagca gcccgcacct ctttttccgg ccaggcggaa
    24301 gggcttccta ccgcacagac attcgaggaa gtgatcaggc atcgaggccg cgtagatgcc
    24361 gcgcaaacac atctgatttt ctacgaggac gagggagaaa gcccgacgct cacgtttggc
    24421 gaactgttgg ccggcgctga gcgcgttgcc gctgatctcg cccggcgcgg cattggacgc
    24481 ggcgacaccg ttgcgttgat gttgcctact tcgcgcgatt tttttctttc ttttgccggt
    24541 acgcttctcg caggggcgat tcctgtgccg atttatccgc cgtttcgcgc cgatcgcatt
    24601 tcggaatatg ccgaacgcca atccgcaatt ctcgccaacg cgggtgcgcg tctgctggtt
    24661 acatttcgcg aagcagccag cgctgccaaa ttattgaaac cgcttgtgcc atctatcgag
    24721 agcgtggtga cggccgatac tctggtccgc tccgatgccg caattcctct cggaacacta
    24781 ctgcactcgc gcgcgaatga tctcgcgctt ctgcaataca cctccggctc gactggcaat
    24841 cccaaaggtg tgatgctgac gcacgctaat ttgctggcga atgtgcgctc catcggtgaa
    24901 gcgcttggca tgcgcaacga agacgtgggc gtaagctggc tgccgctcta tcacgatatg
    24961 ggactaatcg gcgcgtggct tatgccgctg tatttcggat tgcctgtcgt ggtgatgtct
    25021 cccctcgcgt ttctttcgcg gccggcgcga tggctgcgcg cattccatcg ttttcgcggc
    25081 acgattggag cggctccaaa tttcgcgtac gagctggcag ctgccaaaat ttcagatgag
    25141 gatattcagg gcgtagacct cagcccgtgg cgcgcggcgc tcaatggcgc ggaatccgta
    25201 ctgcctgcaa ctctcaatcg cttcgtcgca cggttcgccg cttacggttt tcgccgagaa
    25261 actttattgc cggtgtacgg cctggcggaa gcttcattag ccgtaacaat tccgcaagta
    25321 ggtcgcggtc ccttagtcga tcggctcgac cgcacagcgt tcgcgcaagg aggccgggcc
    25381 atttccgtgg ctccggaaat tcccgccgaa gatttaaatg taatttcgtt tgtatccgtg
    25441 ggtccgcccg tcccgcgcca tgaagtgcgc atcgtgaacg atcgaggaga aaatttgccg
    25501 gagcggacgg agggacaact ctggtttcgc ggaccttcct ctacgcaggg ctattatcgt
    25561 aatgaagcag caaccgcagc gttgctcccc gaaggacttg ccgcgggctg ggtaaattcc
    25621 ggggatcgcg catactgcgc cgacggcgaa atttatatta ccggccgcgt gaaggacatc
    25681 attattcatg cgggccacaa tctttatccg catgagattg aagattgcgt tgcgcgcgtt
    25741 cccggcgtgc gcaagggttg cgtcgttgcg ttcggcgcga cagatccagt ggcaggcacc
    25801 gagcgcctgg tgatcgttgc cgagtcgcgc gaacgaaatc gcagcttgcg cgagcagctt
    25861 gtccaagcca tcaccgcaca agtcatggaa acgcttgggc aacctcccga caaagtcgaa
    25921 gtggtgccgc cgaatgcgat tccaaaaact tccagcggaa aactgcggcg cgacgcaacg
    25981 aagcaaaggt tcctttccgg caaactatat tccggcgcgc gccctgtgtg gttgcaagtc
    26041 gcctggctcg ccgcagcatc gagcgttgtc cgaatccgcg gcggcgtaac acgagctcgt
    26101 gaatttattt acggattcta cgcgattgcg atgtttgctc tgctgctcct gcctgcatgg
    26161 ctgtacgttt tgatttcgcc ctcgcgaaaa agttcggccc gcgtcacgac ggccgcgttg
    26221 cgcgcctact taaaggtcgc cggatggcaa gtccatgtcg aaggtcggga gcatctgcaa
    26281 cacgcgaagc cgcgcatgtt cgtcgcgaat cacaccagtt tcgcggatat tgtcgtgctt
    26341 atggcggctc tcggaacgga ctaccatttc gtcgcgaaaa cggaagtcat gtcgatgccg
    26401 tttttcggaa cgttccttcg gaaactaggc cactactctt ttcaccgcga agatccacgc
    26461 gcaagactag aacaggcgaa acaagtggaa gaagcccttc accaaggcga atctgttttc
    26521 atttttccag aaggcacatt caacgcgcaa tccggcgtgc gcgcatttca tctcggcgcg
    26581 ttcaaagcgg ccacggctgc gcagcgggaa attgtcccta tcgccctcga tggaactcgc
    26641 cgcgtattgc gagacggcac ctggctcccc cgacccggga aaattagcat tacgatttgt
    26701 ccaccggtca ttcctccccc gtcgaccgaa gattggcaac aaatcgtgca aatgcgggat
    26761 tccatccgcg aaacaatcgc cacgttcgcg cacgaaccgc tcctctaatt gcccattcgg
    26821 tgggcaaccg ctcgacgaaa gccttgcatc acattttaag ttcgaacgcc gtttcatttc
    26881 ggacggagtc gcaagggccg aagcaccgac aacttcggcc cacgacaaat aaccgcaaaa
    26941 tcattcgcaa cctgcccaat caattgtgca atcgaaaaat caccgtaatc gaagtctcaa
    27001 cttccacggg ttcgccgttg agcatcgtcg gccgataccg ccattgcgaa accgcttcga
    27061 gcgcggcatg gacgaggagc ggatggccgc tcaggatatc aagcgaagtg atgcggccgt
    27121 cccggtttat gatggcgtgc aaccgtactt cgccttccgt tttggtttgc attgccaacg
    27181 ccgggtaacg cggctcaacg cgggtaatca gctgcgcctg cagcacccct tcgcttattt
    27241 tcacgggatg tttttctact tgaggaggct gagcaacagg ctcgggttct cttattagtt
    27301 gagctacccc gcctccaggt agcatatcgc cgccggggag atacgggcct tcgaagcctg
    27361 catctggcac gcctggcccc tcctcgttct gaactcttct aacagtaacc ccatacttga
    27421 gttgatgata tgggttagga atctcagatc tgcggttgat ggcgtgcgct gcgtcgattg
    27481 gccctcgttt gggcagcgtt ccgggtcgga aaaaactgag ttgagttgga aggatgggtc
    27541 gaaagtttgg ctgtgcgacg ctgttgaata ggggagccgc cacgagcaaa cccagaatca
    27601 ggatttcgat gaacagcgag ataccgaagg cttctcgccg ggtacgtcga gcgctagaag
    27661 ctgacgatat ttcgccttcc accagacaac tgccgagcat gacttcggat ttttcatgat
    27721 cttgccgcgc attttcccgc attgatgggt gcatagacgc tcctcatttc tgccggccat
    27781 gcccgccgtt cggacattgg ccggtctttc gagatactga ggagcgcgcg ctcacttagt
    27841 tggacaccgt ctgatccgtc tagttcccca aattccgagg ctgcatgatt tagagcagct
    27901 ccacagatga aataaggcat ttgttttcaa ttatttacaa tttataagca ttttatgcac
    27961 cattttcgga tgctcgtgca tcacatatgc agtttgcttt ttgggcgagg tacataaatc
    28021 atgcgtcttc cttctaaaga agctgttggg gttcggtata caggttgggc tgcgctgggc
    28081 cgcgatccgc aagaagacac gctcgaatac gcgtgcccct attgcggtga gcctggcgtg
    28141 aaagcggcgc gacgcacccc ggaccaatat catttcacgg atggtttctt gcaggatttg
    28201 caaggggaaa tggctgcatg ccggacttgc cggcattcgt tgcgaattgc gcctgtcgtc
    28261 ctactgcacg atcaggatga aaagcgccgg tttgaaggcg tcttctcgga tgatctgatt
    28321 ccgagcaaga actagattcc caacagctag atgaaaaacg gaaacgggcc gtctccaatc
    28381 tggagacggc ccgtttttca ttctaattaa tttgggttac acggagcagt ggtcgttgcc
    28441 atgggctaat tgctggtgtg tttggcgcta caccaaaagc gaatccggtt ccatgtggtg
    28501 tttctcaagc ccatttcgga cgcgatcgaa tgcctggtcc acggtatccg caaattccag
    28561 catcccgtac tcctgccggc tgatcatccc ggtgcgcacc atgtgcttcc aatcgagcac
    28621 gcggtcccaa tactttcgcc cgtaaatcaa aataatattg cgccgctgca attttccggt
    28681 ctgcatgagc gtgagcattt cccagagttc atccatcgtg ccgaaccctc cgggaaacac
    28741 gatcagggct ttggcgagtt gcgcgaacca caatttgcgc atgaaaaaat aatgaaacat
    28801 gaagctgagc tctggactga tgtaatcgtt tggccgctgc tcgtggggaa gctcaatact
    28861 gagtcctatg gattttccgc ccgcctcgtt tgcgccacgg tttgccgcct ccataattcc
    28921 agggccgccg cccgaacaaa tcacaaagcg gcgcgggtgc tcgcccaaag acatcgccca
    28981 cgatgtcatc ctgcgtgaca actcacgcgc ttcctcgtaa tagcgggaca tttgcagtgc
    29041 ggatttcgcg tcgctcagag ctgccttata ctgtagcggc gtgagattct gtttttgcac
    29101 ttcttgcagc ctgtgtaaat gctttgtcgc ttgatcgtgc gaaaggattc gcgccgatcc
    29161 gaacatcaca atcgtgtcac ccactttttc gcggcgtagc cgttcgagcg ggtcaatata
    29221 ttcggtggtg aagcgcacag tgcgcgccgg cgagctctcc atgaacaccg gatcgcgata
    29281 cggcggtacg atttccttag gatgactcct gcgcttcatg aatcctccgt tgcaagtgac
    29341 tgcagtctga cattttacgc aacccatgcc aggaaagact aatacctcac cgtggatgtg
    29401 ttccctctat ataccgtttt cgcgtctgct tactcaagtg atacaggtct tcgattcata
    29461 tacagcgtga tcgctgtcac agggttcggc aacactacag cgtaatcgcg cttccaacgt
    29521 agtagactgg agaagcgata aaacactcat gcgaacccca cttatttggc tcggcacact
    29581 gactgcgaca atcatttttt ggtctacacc acttcatgcc cagcaaacac aagggcccat
    29641 ttcacccaaa ccaggagagc cggtccagca atccccggaa ggcaggattc gcgttcgtgt
    29701 gtcgctcgtc agcgcgccgg tcgttgttgt ggatgcaaag ggtcagccta tcctcgatct
    29761 tcagcagaaa gattttcaca ttacggatga gggcgtgggc cagaccatcg aatcgttcga
    29821 tttgggtggg gaaccggtcg ccgcggtttt cgtatttgaa accagctcac ggatccagcc
    29881 gctcttgccg gccatccaaa aatcagcaat cgttttcacg caaactgtgg tcgggccatc
    29941 cggtgaagct gccgtcgtga gctatgacag cagcgtcaat acgctgctgc cgttcagcac
    30001 ggacagcgac agaatcgaaa aaacaatcgc caatctcaag gctggaaata gtggcgcgca
    30061 tctttatgac gcgctctcca gtgcggttgg tttgctgcgc aacggaccca ttgggaagcg
    30121 ccgcgcgatt gtcgtcgtag gcgaagcgaa tgacgatggg agcgagcaga aactgggacg
    30181 ggtgctgcgt gaagcacagc ttgctaacat cgtgatttat agtgtggggc tttccaccac
    30241 ttcggccagc ctgcgcgccc ccgtgacgca atctgctccg cccaccggaa cacccccagg
    30301 aacatttggc gggccatctt tcccgggaac ggcgcaaacg cccacgagcc agcaacagta
    30361 tggcggcaat atcgatcttc taggccttgc ggaatgggcc gtaagaaatg ccacagcggt
    30421 ggtgaaggac cgtcctctcg aagtcgctgc aacggccacg gggggcactt acgaatccgt
    30481 tttccgcgat accaaaattg aaaccgcgat cgacgaaatc ggcggcgaac tcaattcgca
    30541 atacatgctg agctatcgtc ccactagcgc ggagaatcct ggttatcacc agatcaaggt
    30601 cacagtggat cggccaggag tgaaagtgcg aacgcg
//
LOCUS       DASZMB010000212        22303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_208390, whole genome shotgun sequence.
ACCESSION   DASZMB010000212 DASZMB010000000
VERSION     DASZMB010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 22303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22303
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208390"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VG272_09825"
     CDS             complement(<1..586)
                     /locus_tag="VG272_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
                     /protein_id="HWF14020.1"
                     /translation="MKRMGMAVLALGLMAFSVQAQQAPAKAPTTPPAKAPATTPQAKP
                     PAAAPGQGLKTTKEKNSYAIGMEMGKGLKSQGVDLDSEVLIQGLKDALTDAKPLLSEE
                     ELRQVVTALQTEMRQKQMQAQEMAAAENKTEGDAFLAANQKKEGVVVLPDGLQYKVLT
                     AGTGKKAVESDTVLCNYKGTFIDGQEFDSSTQAGK"
     gene            766..996
                     /locus_tag="VG272_09830"
     CDS             766..996
                     /locus_tag="VG272_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14021.1"
                     /translation="MGAPKSRIRHKHFRLDSNKISRAQKVLRTETETETIERALDIVL
                     SEHKKNQLAWSAHQHFLDSGIEVKDVYGQGRE"
     gene            997..1419
                     /locus_tag="VG272_09835"
     CDS             997..1419
                     /locus_tag="VG272_09835"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWF14022.1"
                     /translation="MAAVLFDSSVYIKALRGTPESLLALQKDLSTASLWLSSVVLEEL
                     YAGAGQKGHAAVERLQRDFDKARRILVPTLTDWTDTGKILGRLGSKYGFEEIGRGRLT
                     NDALIAISASRKGITVITDNDRDFARLAEFAAFRWSKP"
     gene            1543..2790
                     /locus_tag="VG272_09840"
     CDS             1543..2790
                     /locus_tag="VG272_09840"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF14023.1"
                     /translation="MPETETSSNAGRIAFTYPDFTLYQLARFLIVAGLEMQSVAVGWQ
                     IYEISHRPLDLGLVGLAQFSPGILLFLATGHAADTFNRRKLLMACYAGFGVCSALLLL
                     YALSGLRAVLPMYGVVVLLGVVRSFNNPASRSLLPQIIPEEHLQNAIAWNSSVNQGGT
                     ILGPSLGGLIYAVANGPAAVYATSMLAAGAAAFLMAQVKLRARPRPKEPVSLKSVLAG
                     LHYIMEKKIILGSISLDLFAVLLGGAIALLPVFAAEVLRTGPWGLGLLRASPAIGAGS
                     MALLLAHRPFRSRAGVTMLWCVGGFGAFTVLFGISRSLVLSMIALMLVGSMDMVSVVV
                     RSTLVQLATPDEMRGRVSAVEMIFIGASNEIGQFESGLTAQWFGAVPAVILGGVGTLL
                     ITALWAWNFPELRRVEKLSSLRP"
     gene            complement(3010..3222)
                     /locus_tag="VG272_09845"
     CDS             complement(3010..3222)
                     /locus_tag="VG272_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HWF14024.1"
                     /translation="MTFENYKIVLYKQEDGSWVAEIPAISGCYALMPTREEALQELNS
                     VFQMVSEEYAEKGHMLPADTTEIVNA"
     gene            complement(3309..4295)
                     /locus_tag="VG272_09850"
     CDS             complement(3309..4295)
                     /locus_tag="VG272_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HWF14025.1"
                     /translation="MNILLITEQRDAQWNKTSFETLAAAQQIATETQSRLAAVVIGKG
                     VAALAGELSASKLEEVLLVEHDLLASYTPDGYTVALKQVIAQAKPDLVLLPHTYQVRD
                     FAPQLAATLGKAMIGDCVGHRHENGKLIFVRQMFQGKTAADVVFEGAAPWFATFQAGA
                     FRADLVTKGVANAPVTSLSVDLKPEQIRTKPLELFREAKQAVDLTQAPILVAVGRGIK
                     APENIALAEKLAKVIGGELAASRPICDEGWLPMDRQIGSSGQTVAPKLYLALGISGAI
                     QHVVGMKGSRTIVAINKDANAPIFEVADYGIVGDLFEIVPALTEELEKSKGA"
     gene            complement(4440..5207)
                     /locus_tag="VG272_09855"
     CDS             complement(4440..5207)
                     /locus_tag="VG272_09855"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HWF14026.1"
                     /translation="MKIVVCIKQVPAKDAPLAITDASWIRETDIGFEMNEPDSYALEE
                     ALRLKEKHTGEVIALSMGPERVKQTIKEALAKGADRGVHIVDDKAHTLDPLGAAKFLA
                     AAIEREKPDLVLTGLQSEDQGFGQTGVLLAELLNRPHGTIIMQIEVQGSGLRVKRELE
                     AGWFQFVEMPMPAVLTIQSGINKVRYATLKGIMAAKKKEIATITRESFGVTHEPTQHI
                     ERIYVPTKSKKTEFVTGTPKEAAAKLVEKLKHEARVL"
     gene            complement(5340..6584)
                     /gene="fabF"
                     /locus_tag="VG272_09860"
     CDS             complement(5340..6584)
                     /gene="fabF"
                     /locus_tag="VG272_09860"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF004970.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HWF14027.1"
                     /translation="MSRRVVVTGVGLVCGLGIGTDDVWKNLLAGMSGVRSITHFDASA
                     FDCTIAGEVRDFDPLHWAEKKEIKKIARFIQLALAATDFAMRMSELKITSDIEEVTGV
                     YVASGIGGFDVIEREHSKLLQGGPGRISPFFIPAAIINLASGHISIRYGAKGPNSATA
                     TACSASAHAVGDSFRLIQHGDAEVMICGGAEAAITPMGVGGFAAMKALSTRNNEPERA
                     SRPFDANRDGFVIGEGAGILVLESLEYAERRGAKIIAEIVGYGMSGDAHHITAPEENG
                     DGAYRVMRAAIRDARIEPSQVDYVNAHGTSTPLGDAIETKAMKRVFGEHTKKIAVSST
                     KSMTGHLLGGAGGLEAGISILALRDQVLPPTINQETPDPECDLDYIPNHMRKAGVQHA
                     LSNSFGFGGTNAALLFKRWDSR"
     gene            complement(6626..6865)
                     /gene="acpP"
                     /locus_tag="VG272_09865"
     CDS             complement(6626..6865)
                     /gene="acpP"
                     /locus_tag="VG272_09865"
                     /inference="COORDINATES: protein motif:HMM:NF002149.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWF14028.1"
                     /translation="MASVDERVKQIIVEQLGVDEGEVTATASFVDDLGADSLDTVELV
                     MAFEEAFGIEIPDEDAEKIATVKDAVDYITKHGKK"
     gene            complement(7080..7826)
                     /gene="fabG"
                     /locus_tag="VG272_09870"
     CDS             complement(7080..7826)
                     /gene="fabG"
                     /locus_tag="VG272_09870"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HWF14029.1"
                     /translation="MFSLKDRVALVTGASQGIGRVTALALAKAGASVVVAARNTEKLA
                     GVVAEIEAAGGQSLAVAMDVANAEQVKSGFRLAIEKFSKLDILVNNAAITRDGLALRM
                     KADDWDAVLRTNLTGAHLCSQQAMATMMRARYGRIINVTSVVAETGNAGQSNYVAAKA
                     GLIGLTRALALEIASRNITVNAVAPGFVVSPMTDPLPQSVKDALLARVPLGRMGCDSE
                     VAAAIVFLASEEAGYITGAVLDVNGGLRMG"
     gene            complement(7908..8867)
                     /gene="fabD"
                     /locus_tag="VG272_09875"
     CDS             complement(7908..8867)
                     /gene="fabD"
                     /locus_tag="VG272_09875"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HWF14030.1"
                     /translation="MGKLAFLFPGQASQYPGMGKELAANHPAARSVFEEADKALGFSI
                     SEICFSGSEEALKLTANTQPAILTVSVAAYRVLEENGVQPAFVAGHSLGEYSALVAAG
                     ALDFADAVRLVRHRGEYMQTAVPEGVGAMAAILGLAPAQVAGICRQAADGQVVAPGNL
                     NSPEQTVISGNAEAVKRAVESASAGGAKRAVMLPVSAPFHSALMKPAAERLDVDLRAT
                     EFHNLRIPLVNNADAEITISGDEAREGLIRQVTLPVRWEESIRELIDQGVNTFVEVGP
                     GRVLTGLLRQIERSVHVFNVEDEKSLRSTLEKLSQAQTESAEA"
     gene            complement(8891..9904)
                     /gene="plsX"
                     /locus_tag="VG272_09880"
     CDS             complement(8891..9904)
                     /gene="plsX"
                     /locus_tag="VG272_09880"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HWF14031.1"
                     /translation="MITIAVDAMGGDHAPRPEVEGAVQAARELGVRILLVGLMPEVKK
                     ELSKHSHRGLPIEIVPATEVITMDDSPTVAFRKKKDSSAHVATKLVRTGHADAIVSAG
                     NTGAVMAVARFGLGTLSSVDRVALAAPFPTSKGGTAVLLDVGANVDSKPAHLVQFAVM
                     GEIYYRAIFGTRRPKVALLSIGEEESKGNELTREAYSRLKVSPLNFAGNVEGRDIFNG
                     SVDVIVCDGFVGNIALKISEGVAMHIAGMLKEALSSTISSKVGYVLSSKAYKSFRKKI
                     DYSEYGGAPLLGVKGVTVIGHGSSNPLAIKNAIRVATELVRGGVNDRIEQELTSIPVP
                     VEA"
     gene            complement(10194..10727)
                     /locus_tag="VG272_09885"
     CDS             complement(10194..10727)
                     /locus_tag="VG272_09885"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HWF14032.1"
                     /translation="MFLDVKELAIHKIRIRKSYAPGTVDYRTGDFRQAEPLEVRATAE
                     LVEGQIRVTGELHTRLEMVCARCLEPVHEDIAREFDLFYKPVLTMTKEEEERLRLDDT
                     EIAFFEGQGMFLADVLAEQVILALPMKAICRSDCRGLCPQCGVNLNNEECRCESHTAD
                     PRTAPLARLKQDWFKKQ"
     gene            complement(10962..12098)
                     /locus_tag="VG272_09890"
     CDS             complement(10962..12098)
                     /locus_tag="VG272_09890"
                     /inference="COORDINATES: protein motif:HMM:NF002326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HWF14033.1"
                     /translation="MALASYAMDVAKSRGRRYPEPQHPYRNDYARDRDRVIHSRAFRR
                     LEAKTQVFTTRYSDHFRNRLTHTLEVAQVARTVAGALQLNSDLAEALALVHDIGHPPF
                     GHAGEHKLDELLRPHGESFNHNLQALHLVEQFEQRYLDFPGLNLTFEVREGIVKHSRD
                     YDAAQFPALSEYLLDQRPPIEAQLIDFVDEIAYNTADLDDGYEAHILNIEMICTEVPL
                     FARFYRQVEREYPQGLEKLKFNEAAKRVLNYLATGLIENTQTAITVSGVKTVEDVRHA
                     PRRFAGFSATMAAENGALKKFLIARLYSQPAIVVDRERSVDALEQLFNFYVEHPEAMP
                     KTYADLAKQGDKYRVVCDYIAGMTDHYLLRLHAELLGAKEVRSA"
     gene            12260..12646
                     /locus_tag="VG272_09895"
     CDS             12260..12646
                     /locus_tag="VG272_09895"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HWF14034.1"
                     /translation="MKIKLLMAFILVLCGCVIAAPLFAHHSSSAYDMSHPVDMKGVVT
                     NMEWSNPHVFIYMNMKNDKGEVEEWRVEGNSPNMLFRAGWKKEMIKAGDELSVNGAPA
                     KNGAKSLRLISLTLANGQKYDGQGFK"
     gene            12683..13402
                     /locus_tag="VG272_09900"
     CDS             12683..13402
                     /locus_tag="VG272_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14035.1"
                     /translation="MRNCSVVLLSAACILVLPPAISAQAKPPAAPAKTHSASAKPDLS
                     GVWFIDEYHRNLLPNEDPPFQPWAKDLFKKRQEESANRTPDSGPDPTEKCIPPGTPRE
                     MFQPFPWEIVYARDRILMLFEYQGLIRQIFTDGRGHPKDLEPTYMGHSIGKFEGNTLV
                     VDTIGFNDKTWLDPMGLPHSDAMHLTERIRRVNHDTLVDEYTIDDPKAYTKPWKAQRT
                     FVLKPDWQIQEYVCAENNNVK"
     gene            complement(13552..14268)
                     /locus_tag="VG272_09905"
     CDS             complement(13552..14268)
                     /locus_tag="VG272_09905"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF14036.1"
                     /translation="MAQHILRRDLKKDEIREKMVSGFETVATHQQAMWIVIIAALVVA
                     LAVLGWDSYSRRQTAKASLALDDALKIYQARIRAAGEPADPTEITYLDEKNKFTDAEK
                     KFRAVAADFGRTKPGQMAEYYAALSEVQLKNYADAEKNLSQVISGGDENLASLAKYQM
                     AQVYQQENKGSQAVDLLKQLSDKPSIMVPKAMSMLALADYYRKSDPAQATKLYNQVKQ
                     EYPAAAAEADQGLELLNAKS"
     gene            14526..15053
                     /locus_tag="VG272_09910"
     CDS             14526..15053
                     /locus_tag="VG272_09910"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWF14037.1"
                     /translation="MDFRRKNIRLHPTRYKGNGWYFITICCEARKCIFPEPKQGDKII
                     DLIKIASEKYKFGVHAYCVMPDHFHVLVEGLASDSDLLLFVRSFKQSSTRAFSGGNGI
                     PLWQKKFYDHILRAEESPEAVAWYIWMNPVRKGFCSNPDEYLYSGSLTTDWKKMVRTP
                     ESWVPAWKQTQVARP"
     gene            complement(15150..15359)
                     /locus_tag="VG272_09915"
     CDS             complement(15150..15359)
                     /locus_tag="VG272_09915"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HWF14038.1"
                     /translation="MSREQGVVKWFNASKGYGFIQRQSGEDVFVHFSAIQMDGYKTLN
                     EGQMVEFEVKQGPKGYQAESVTAAS"
     gene            complement(15680..16027)
                     /locus_tag="VG272_09920"
     CDS             complement(15680..16027)
                     /locus_tag="VG272_09920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWF14039.1"
                     /translation="MSFKATSREIGSVTVIDMDGRITLGEGSALLRDLIHENLAKGHK
                     KIVMNLAGINYIDSSGLGELVSGYRLIKSEGGEVKLLNLNKKVTDLLQITKLYTVFDI
                     HNQEEQALASFQS"
     gene            complement(16099..16524)
                     /locus_tag="VG272_09925"
     CDS             complement(16099..16524)
                     /locus_tag="VG272_09925"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF14040.1"
                     /translation="MPSKPQRIEVVLETLIESIALAEEMGVRAAAAAGFGEDDQYKIG
                     LAVHEGVMNAFQYGNQERRERKIHLTFELHEEKIVIHVVDQGAGFRLEDVPDPRHDEN
                     VLSDSGRGVLLMRAFMDEVDVVSSAAGGAEVVMAKHHKH"
     gene            16679..17425
                     /locus_tag="VG272_09930"
     CDS             16679..17425
                     /locus_tag="VG272_09930"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWF14041.1"
                     /translation="MNSYLLLSIAGLAAGLINSVAGGGMFITFPALVFTGVPSIIANA
                     SSTIALIPGVLASAGAYRHDFRKSENFPFIPLLVVSVAGGIVGALLLLFTPQSTFDSV
                     IPWLLLVATLLITFGPRISPLLKRVIHIGPVAVVVIQFFIAIYGGYFGGAIGILMLAT
                     WTVFGLTDIHVMNANRTLMGAAANSIAAVLFIVAHKVWWPQTLVMLVFCSIGGYIGAV
                     AAKKVHPSYVRVFVILISTSITIAFFLRKH"
     gene            17667..17867
                     /locus_tag="VG272_09935"
     CDS             17667..17867
                     /locus_tag="VG272_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14042.1"
                     /translation="MSKIDELKAEIENLSSNEVAEIYRWLSEKDWERWDKEIEDDSQS
                     QKIDFLLREAHEEKVKGNLKDL"
     gene            17868..18128
                     /locus_tag="VG272_09940"
     CDS             17868..18128
                     /locus_tag="VG272_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14043.1"
                     /translation="MHHATSRFWNSFGLLPENIQEIARKNFSLLKNDPSHPSLHFKKV
                     GKLWSARVGTHHRALAVPDRADFLWVWIGTHDEYMRLIKQEG"
     gene            complement(18131..19756)
                     /locus_tag="VG272_09945"
     CDS             complement(18131..19756)
                     /locus_tag="VG272_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWF14044.1"
                     /translation="MLSLNNITMRYGARLLFEEVTCTFLEGRRYAISGPNGAGKTTLM
                     KILEGDLEPTTGSVSRPKKLGILRQDQFAFDQFRVIDTVIMGNGPLWKALQERDILYA
                     KPHEELTDKDGMRLGELEGVVGDEGGYTAEADAAILLDGLGVEAALHERNMSELQGGQ
                     KVRVLLAQALFGNPAALLLDEPTNHLDLDSVHWLQDYLHEYQGVLIVISHDRHFLNSV
                     CTHTADIDYQTVIMYTGGYDEMVLAKTQIRSRIEADNAVREKKIAQLNEFIARFSAGT
                     RSSQVTSRKKEVERIQTSELARSNIARPYIRFDMKIPSGRHPLEFKSLTKTYDGHKVI
                     DKFTANVERGEKIAIIGRNGAGKTTLIKSLVRSAVGDIEDSDRQFPVDGGHVKWGHEA
                     VVGYFAQDTTDTIAKNTNVIEWLHQFDPKASQQELRAVLGQMLFSGEDAEKSTRALSG
                     GEQARLIFCKLMLQKPNVLVFDEPTNHLDLESINALNIALQKFPGTVIFVTHDHDVID
                     EVATRIWQCKGGHVEDFKGPYEDFLVYAKQKAS"
     gene            20180..21160
                     /locus_tag="VG272_09950"
     CDS             20180..21160
                     /locus_tag="VG272_09950"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWF14045.1"
                     /translation="MKMSRVILGLTLLTVASVNAAPQQQKKVAAEPPAWAYPINPPDD
                     RPAADDGSLHHVPGSSKGLTLTQITDWFNIPDWHPEGHPAMPEIIAHGRKPAVRGCGY
                     CHLPNAQGRPENASLAGLPASYIVQQVMEIKSGVRRCSEPRMGPPGNMVSIAQSASLE
                     DAKAAAEYFSSLQLKPWIKVIETDSVMKTKVKGSMLVAADGGGKEPIGHRIVETPANL
                     ELTELRDDASGFIAYVPVGSIDKGKTLVMAGGEGKTTRCWFCHGPELRGLGPVPRLAG
                     RSPSYLFRQLYDFQTGNRHGEWSALMKDPVAHLTNDDMLNIAAYLASLEP"
     gene            complement(21308..>22303)
                     /locus_tag="VG272_09955"
     CDS             complement(21308..>22303)
                     /locus_tag="VG272_09955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HWF14046.1"
                     /translation="SSMLADASQYAVDMRDVRGQQAAKRALEVACAGGHNIIFIGPPG
                     AGKTMLAKRIPTILPSMSLEEAIETTRIHSVAGLLDDARGLVGTRPFRSPHHTISDAG
                     LIGGGAIPRPGEVSLAHHGVLFLDELPEFERNVLEVMPQPLEEGAVTISRAATSITFP
                     ARFMLAAAMNPCPCGFFGDPTRQCHCSPPQIQRYVSKISGPLIDRIDIHIEVPAVKYK
                     ELRGNGNIEDSAKVRERVLRARERQSKRYAGERKVFSNAHMPPKLIRKFCAISEEGEK
                     MLENAIGRLGLSARAHDRILKVARTIADLDGSENLETRHLGEAIQYRTLDRSYWA"
BASE COUNT         4948 a         6318 c         5679 g         5358 t
ORIGIN      
        1 gctttccggc ttgcgtggaa ctgtcaaact cttggccatc aataaacgta cccttgtaat
       61 tgcacaaaac tgtgtcgctt tccacggcct ttttgccagt gcctgccgtc aaaaccttgt
      121 actgcaatcc atcgggcaac accacaacgc cttctttctt ttggtttgcc gcgagaaacg
      181 catcgccctc ggtcttattt tccgccgctg ccatttcctg cgcctgcatc tgcttctggc
      241 gcatttcggt ctgcagcgcg gtgacgacct ggcgcagttc ttcttcgctc aacagtggtt
      301 tcgcatcggt gagcgcatct ttcaaacctt gaattagcac ctcggaatcc agatcgactc
      361 cctggctctt caagcccttg cccatctcca ttccgatggc gtagctattc ttttccttgg
      421 ttgtcttcaa tccttgtcct ggtgccgccg cgggcggctt cgcctgtggc gtggtcgccg
      481 gtgcctttgc gggcggcgtc gttggcgctt tcgcgggcgc ttgctgtgct tgtacgctaa
      541 aggccatgag cccgagcgcc agtactgcca ttcccatccg cttcatccgt cctcctagat
      601 tgaacgacga gcgttgcttt ggcgctcatg ttccacccta gacggtaacc gatgtcaccg
      661 ttccgccgca agttttagaa gtgtgattct ttgagcaaat ttaatgttca tattcaccat
      721 atttatgtac atggatgtta caataacaat tgcgaggcag accgaatggg tgcaccaaaa
      781 tcacggattc gacataaaca tttccgtctg gattcaaaca agatttcgcg cgctcaaaag
      841 gttctgcgca cagaaacaga aacggaaacg atcgagcggg ctctggacat cgtgctctcc
      901 gagcacaaga aaaaccagct agcctggagc gctcatcagc atttccttga cagcggcata
      961 gaagttaagg acgtttacgg ccaagggaga gagtaattgg cggctgtatt gtttgattct
     1021 tcagtttata taaaagctct ccgtggaact ccagaatcgc tcctcgctct tcaaaaagat
     1081 ctctcaacgg catccttgtg gctcagttca gtggtccttg aagaactgta tgcaggggcg
     1141 ggtcagaagg gtcacgccgc ggttgaacgc ttgcagcggg atttcgacaa agcccgccga
     1201 atactagtgc caacgttgac cgattggacc gacactggca aaatcctggg gcgtctcggc
     1261 tccaaatacg gttttgagga aattggcagg ggacgcctca caaatgacgc tttgatcgcg
     1321 ataagcgcca gccggaaagg aatcactgtc attaccgaca atgaccgcga cttcgccagg
     1381 ctcgccgaat ttgccgcttt cagatggtca aaaccataat cgctcttcgt tttagcttat
     1441 ggggaagatg caaccactat gccactttta agccaaacat aatctgacac tttgacctcc
     1501 gctcgtgcaa atcccatttt tatggcaaag tagattcctc tgatgcccga aaccgaaacc
     1561 tcctcaaacg cgggacgcat agcgttcacc tatcccgact tcacccttta tcaattggca
     1621 cgattcctga tcgttgctgg attggaaatg cagtctgtcg cggtgggctg gcaaatctat
     1681 gaaatttcgc accgtccgct tgacctcggg cttgttggac tggctcagtt ttcccctggc
     1741 attctgctat tccttgccac gggacacgcg gcagatacgt ttaaccgtcg aaaattgttg
     1801 atggcctgct atgcgggttt tggcgtctgc tcggcgctgc tgcttctcta cgccttgagc
     1861 ggattgcgcg ccgtgctgcc catgtatggc gttgtggttc tactgggagt tgtgcggtcc
     1921 ttcaacaatc ccgccagccg atccctcttg ccgcaaatta tccctgaaga acatttgcag
     1981 aacgccatcg cctggaacag ttccgtgaac cagggcggca cgatcctcgg accttcgctt
     2041 ggcggtctga tctatgccgt cgccaatggt cctgcagcag tctacgccac ctccatgctg
     2101 gccgcggggg ccgcagcctt cctcatggct caagtaaagc tccgcgcacg accgcggccg
     2161 aaggagcccg tcagcctcaa atccgtgctg gccggactcc actacatcat ggaaaagaaa
     2221 attattctgg gatccatctc gctggacctc ttcgctgtct tgctgggcgg cgccatcgcg
     2281 ctcttgcccg tctttgcggc ggaagttctt cgaacaggtc catggggcct gggacttctg
     2341 cgtgcatccc ccgccatcgg tgccggtagc atggcattgc tgctcgcgca taggcccttt
     2401 cgcagccgcg cgggcgtaac catgctgtgg tgcgtcggcg gctttggcgc tttcaccgtt
     2461 ctatttggga tatctcgaag cctggttctg tcgatgattg cactgatgct ggtcggatcg
     2521 atggacatgg tcagcgttgt ggttcggtcc acgctcgtgc aactggctac gccggacgaa
     2581 atgcgcggac gcgtcagcgc cgtcgaaatg attttcatcg gcgcttcgaa cgaaatcggc
     2641 caattcgaat caggcctgac cgcgcaatgg ttcggcgcag tccccgccgt aatcttgggt
     2701 ggagtcggca ccctcctcat aaccgcattg tgggcgtgga atttcccaga gctgcgccgc
     2761 gtagaaaagc taagctcgct aaggccttag gtcgatttcc aattaattaa ttcgttgaaa
     2821 ctcatccaga gaaattccga gctgccgcaa aatttgaaaa acaacggagg accaatctca
     2881 cgacctgcat gaagtggtat tgtaacggca cgaccatcgg gatgattcca gcgttcgtga
     2941 ctgccagtct ggcgggttcg aagaaatcca agcttcctgg ccgcgttttg aaattcacgc
     3001 gctgtggcgc taggcattca cgatttccgt tgtatcagct gggagcatgt ggcccttctc
     3061 cgcatactcc tcggacacca tctgaaacac ggagttgagt tcttgaagag cttcttcgcg
     3121 ggtgggcatt aaggcatagc atccggaaat agccggaatt tcggctaccc aagatccatc
     3181 ttcctgcttg taaaggacaa ttttgtaatt ttcaaaggtc atctgatgct cacctgaata
     3241 gtgagtttag cacaaagaca ctttgatctt catgcgagat ctatcgcagt tttcatctgt
     3301 aaacgaagtt acgctccctt cgacttttct aattcttccg taagcgcggg aacgatttca
     3361 aacaaatctc cgacaattcc ataatcggca acttcaaaaa tcggcgcgtt cgcgtctttg
     3421 ttgatcgcga caatcgtgcg cgaacccttc atccccacaa cgtgctgaat cgctccgctg
     3481 attcctaggg caaggtacaa cttgggcgcg acagtctgcc ccgaactacc gatctggcga
     3541 tccatgggca gccacccttc gtcgcagatg ggtcgcgatg cggccaattc tccgccaatc
     3601 acttttgcca gcttttccgc gagagcaata ttttccggcg ctttaatgcc gcgcccgacc
     3661 gccactagaa tgggcgcctg cgtgagatca accgcctgct tggcttcgcg gaatagttcg
     3721 agcggtttcg ttcgaatttg ttccggctta aggtctactg ataaagaggt taccggcgca
     3781 ttcgctacgc ccttcgtcac aaggtccgcg cgaaacgcgc ccgcctggaa agttgcaaac
     3841 cagggcgcag cgccttcgaa caccacatcg gccgctgttt tcccttgaaa catctggcgc
     3901 acaaaaatca atttcccatt ttcatggcgg tgtcccacgc aatcgccaat catggccttg
     3961 ccaagcgttg cggccagctg gggcgcgaaa tcgcgcactt gatacgtgtg gggcagcagg
     4021 accaaatccg gcttcgcctg cgcgattact tgcttcaggg caacggtgta tccgtccggc
     4081 gtatacgaag ctaacaaatc gtgctcgacg agcagtactt cttcaagctt gcttgccgaa
     4141 agttccccag ccaaggctgc aacgcctttg ccaatgacca cggccgcgag gcgactctgc
     4201 gtttccgttg caatctgttg cgcggccgcc agcgtctcga aacttgtctt gttccactgc
     4261 gcgtcacgct gttcggtgat caggagaata ttcattgtgg tccaatctcg cccagcctgt
     4321 atttcaaaac catccgctga aacttccccg cgaattcagg atatgtccat gagtgaagat
     4381 gttttcgcat tggtaggggc atggcatgcc gtgctcctac ggcgaacccg gcgcgcttgc
     4441 tataacaccc gagcttcgtg tttcaatttc tcaaccagtt ttgctgccgc ttctttcggc
     4501 gtgccggtta caaattcagt cttcttggat ttagtcggca catagatacg ttcgatgtgt
     4561 tgcgtcggct cgtgtgtgac gccgaacgat tcccgcgtga tagtcgcgat ttcttttttc
     4621 ttcgcggcca tgatgccttt gagcgttgca tagcgcactt tattaattcc ggactgaatc
     4681 gtcagcaccg caggcatcgg catctcgaca aattgaaacc atcccgcttc gagttcgcgc
     4741 ttcacgcgca agccactgcc ctgcacctca atctgcatga tgatcgtgcc atgcggccgg
     4801 ttcagcaatt ccgcgagcag cacgcccgtc tgcccgaaac cctgatcctc gctttgcaat
     4861 cctgtcaaca cgaggtccgg tttttcgcgc tcgatggccg ccgccaaaaa tttggccgcg
     4921 cccagcggat ctaacgtgtg cgctttatcg tccacgatat gcacgccgcg atcggctccc
     4981 ttggcgagag cttccttgat tgtctgcttc acacgctcgg gccccatcga caaggcaatc
     5041 acttcgcccg tgtgcttttc cttcaggcgc aaagcctctt cgagcgcgta actatccggc
     5101 tcattcatct cgaaaccgat atcggtttcg cggatccagc ttgcatcggt aatggccaga
     5161 ggagcgtcct tggcgggcac ttgcttaata caaacgacga tcttcatccg taccccttgt
     5221 attgctgggt tgctgttggg cttgtgtagc gcccgccttc aggcgggcaa cgggtgatca
     5281 ctcgatgcca acctgaaggt tggcactaca aaatcgcggc aagcaccaaa aaaattgtcc
     5341 tagcgggaat cccagcgctt gaagagcagc gcggcgttcg ttccgccgaa cccgaaagag
     5401 ttcgagagcg catgttgaac gcctgcctta cgcatatggt tcgggatgta atcgaggtcg
     5461 cattcagggt ccggcgtttc ctgattgatc gtgggaggca gcacctgatc gcgaagcgcc
     5521 aggatgctga tccctgcttc caggccgccc gcgccgccga gcagatgccc ggtcatggac
     5581 ttcgttgagc tgaccgcaat ttttttggtg tgctcgccga acacccgctt catggccttg
     5641 gtttcgatcg catcgcccag cggcgtcgaa gttccatgtg cattgacgta atcgacctga
     5701 ctcggctcaa tcctcgcatc gcggattgcc gcgcgcatga cgcgatatgc gccatcgcca
     5761 ttttcttccg gcgctgtaat gtgatgtgcg tcgccagaca ttccgtagcc gacgatttcc
     5821 gcaataattt tcgctccgcg ccgttccgca tattcgagcg actcgagaac cagaattccc
     5881 gcgccttcgc cgatcacaaa cccgtcgcgg ttggcgtcaa acgggcggct ggcacgttcc
     5941 ggttcattat tccgcgtgga gagcgccttc atcgcggcga atccgccaac gcccattggc
     6001 gtgatggctg cttccgcgcc gccgcaaatc atcacttctg catcgccgtg ttgaatcagg
     6061 cggaaggaat cacccacagc atgcgccgat gcggaacacg ctgtagccgt ggccgaattc
     6121 ggacccttgg cgccgtatcg tatggaaatg tgccccgagg caaggttaat gatcgccgca
     6181 ggaataaaaa atggggaaat tcgtccgggt ccgccttgca ggagtttcga atgctcgcgt
     6241 tcgatcacat cgaatccgcc gattccgctc gctacgtata cacccgtcac ttcttcaata
     6301 tcggacgtga ttttcaattc tgacatgcgc atggcgaaat ccgttgccgc cagcgcaagc
     6361 tggatgaacc gggcgatttt cttgatttct tttttttcag cccagtgtag aggatcgaaa
     6421 tcgcgaactt ctccggctat cgtgcagtca aaagcggagg cgtcgaaatg tgtgatcgac
     6481 cggacgccgc tcattccggc caggagattt ttccagacat catccgtgcc gatacccaga
     6541 ccacagacta gtccgacacc cgttaccact acccggcggc tcacttgatc cacactctcc
     6601 tggtgatttt attgcggccc gtgtactact tctttccgtg cttggtgatg taatcgaccg
     6661 cgtcttttac cgtggcgatt ttctcggcat cttcgtcggg aatttcgatt ccgaatgcct
     6721 cttcaaatgc cattaccaat tcgaccgtat ccagggaatc cgcacccaga tcgtctacga
     6781 aggaagcggt ggcagtgact tcgccttcat ccactccaag ctgttccacg ataatctgtt
     6841 ttacgcgttc atcgacactg gccattctgt tttgcctccg ataatctgtg tcaagctctt
     6901 tgcccatctt gtcgcgggcc ggtccttcgt acaaattggt aatgatttta agcacggaca
     6961 ttctatgtat ggcatcggcc taggtcaact taactctgaa tctgaccata agcaaattcc
     7021 aaccctgctt acctgcgctg ccgccttgcc gcacatgttt ccggctccgg gcggcattcc
     7081 taccccatgc gcagcccacc gtttacgtcc agcaccgcgc cggtaatata acccgcttct
     7141 tcactggcaa gaaacacgat ggcggcagca acttccgaat cgcatcccat gcggccgagc
     7201 ggtactcgcg cgagcaacgc atccttcaca ctttgcggca acggatcggt catgggagaa
     7261 acgacgaacc ccggcgccac ggcattcact gtgatattgc gcgaagcgat ttccagggcc
     7321 agcgcgcgcg tcaaaccgat cagcccagcc tttgctgcca cgtaattaga ctgccccgcg
     7381 tttcctgtct ccgcgacgac cgaagtcacg ttgatgatgc gcccataccg cgcacgcatc
     7441 atcgtggcca tggcttgctg cgagcacaaa tgcgcccccg tcaaattcgt gcgcaatacc
     7501 gcatcccaat cgtcggcctt catgcgcaaa gccaggccat cgcgcgtaat cgccgcgtta
     7561 ttcaccagaa tgtccagctt gctgaatttt tcaatcgcta aacgaaatcc cgatttcacc
     7621 tgctcggcat tcgcaacatc catcgccacg gcaagcgatt gtccgcccgc cgcttcaatt
     7681 tcagcaacca cgccagccaa tttttccgtg ttccgcgcag ctaccaccac gctcgcgccg
     7741 gccttcgcca gtgcaagcgc cgtgacccgc ccaattcctt gggacgcacc ggtcaccaat
     7801 gctacgcgat cttttagact gaacatggat atttttgtgg gtagccttca acctgcgggg
     7861 tttagtgcta gcaaggacta aaatccacag gctgaagcct atgccaccta ggcctcagca
     7921 gattcagtct gcgcctgcga aagtttctcc agcgttgagc gcagagattt ttcatcctcg
     7981 acgttaaaca cgtggaccga ccgctcgatc tgccgcaaca atccggtgag cacacgtcca
     8041 ggccccactt ccacaaacgt gttaaccccc tgatcgatca actcgcgtat cgactcttcc
     8101 caacgtacag gcaatgtcac ctgacgaatc aggccttcac gcgcttcatc gccactgatg
     8161 gtaatttccg cgtccgcgtt gtttactaaa ggaatccgca aattgtgaaa ctctgtcgca
     8221 cgcagatcga catcgagacg ctccgcagcg ggcttcatca gcgcgctgtg aaatggagcc
     8281 gacaccggaa gcatcacggc acgctttgcg ccacctgcag atgcggactc caccgcgcgt
     8341 ttcaccgctt ctgcattacc cgaaatcacc gtctgctcag gagaattcag attgcccggc
     8401 gccaccactt gtccatccgc cgcttgcctg caaattcccg cgacctgcgc gggagctaac
     8461 cctaaaattg cggccatcgc gcccacgcct tcgggcactg ccgtctgcat atattcgccg
     8521 cggtgccgca cgagacgcac agcgtccgca aagtccagcg caccggctgc cacgagcgcg
     8581 gaatattcgc ccaagctatg ccctgcaaca aatgccggct gcactccatt ttcctcaagc
     8641 acacgatacg cagcgactga cacagtcagg atcgccggct gcgtgttcgc cgtcaacttc
     8701 aacgcttctt cgctgccgct gaagcatatt tcggaaatag agaagcccag tgccttgtca
     8761 gcctcttcaa acacacttcg agcagccgga tggttagcgg ccagttcttt gcccataccc
     8821 gggtattgag aggcttgccc cgggaacaaa aatgctaatt ttcccattga aaataccgcc
     8881 gtttcgtcgc ttatgcttcc acaggaacgg ggatgctcgt aagctcctgc tcgattcggt
     8941 cgttgacgcc gccccgaact aattccgttg ccacgcgaat cgcatttttg atcgccaggg
     9001 gattggaact cccatgcccg atcacggtca cgccctttac ccctagtaaa ggagcgccgc
     9061 cgtattccga ataatcaatc ttctttcgaa aactcttgta cgccttgctc gaaagtacgt
     9121 agccaacttt agagctgatc gtgctggaaa gcgcttcctt cagcattcca gcaatgtgca
     9181 tcgcaacgcc ttcgctgatc ttcaaggcaa tgttgccgac gaacccatcg caaacgatga
     9241 cgtccacgga gccgttgaat atatcgcgcc cttccacgtt tcctgcaaaa ttcaatggag
     9301 aaactttcaa ccggctgtat gcctcgcgcg tcagctcatt tcccttggac tcttcttcac
     9361 cgatcgatag caatgcgacc tttggccgcc gcgttccaaa aattgcgcga taataaattt
     9421 cgcccatcac cgcaaactgc acgagatgcg cgggcttcga atccacattc gcgcctacgt
     9481 ccaataaaac tgccgtgcca ccctttgaag tcgggaatgg agctgccagc gccacgcggt
     9541 ccaccgacga gagtgtgcca agcccgaatc gagctacggc catcacggca cctgtattcc
     9601 ctgcgctgac gatggcatcc gcatggcctg ttcgaaccaa ctttgtcgcg acgtgtgccg
     9661 aactatcctt cttcttacga aaagcgaccg tcggtgagtc gtccatcgtg atgacttccg
     9721 tcgccggaac aatttcaatg ggcaaaccgc gatgcgagtg cttcgaaagc tccttcttca
     9781 cttcaggcat gagccctacg aggagaattc gaacacccaa ctcccgtgca gcctgcactg
     9841 ctccctcgac ctcaggacgc ggcgcatggt ctccacccat cgcatctacg gctatggtga
     9901 tcattcaaac tccggtaggg gaattgcgta aaggccgaac acgaccgggc gtcaggaccc
     9961 ggtgtccacc acttcccggc ctttatagta accgcagttc ggacatgact ggtgaggctg
    10021 cttcatctcg tgacagttag gacacttggc aagcgccgga ggtgtcagaa aatcatgggc
    10081 gcgccgcttg ttctttcgcg cgtgggagtg acgtcgtttt ggattgggca tgcaaactcc
    10141 ttcttatggc cccaaataga atgaggcatt ctatggtgtt tcgtctgaga ttcctattgc
    10201 tttttgaacc agtcctgttt caggcgcgcc aatggcgcag tacgcggatc agctgtatgg
    10261 ctttcacagc ggcattcctc gttattcaag ttcacaccgc actgggggca taagccacgg
    10321 caatcactgc ggcagatcgc tttcatcggg agggccaaga tcacctgttc ggccagaaca
    10381 tcggccagaa acatgccttg gccctcaaaa aacgcgattt cggtatcgtc cagccttagg
    10441 cgttcctctt cttccttggt catggtcaac acgggcttat agaagaggtc aaattcgcgg
    10501 gcaatatcct cgtgcacggg ctccaggcaa cgcgcgcaca ccatttccag acgggtgtgc
    10561 agttcgccag ttacgcgaat ctgcccttcc accagttcgg ccgtggcacg cacttcaaga
    10621 ggctccgcct gccggaagtc gcccgtacgg taatcgaccg tgcccggggc gtagcttttc
    10681 cggatgcgaa tcttatggat tgccagttct tttacgtcta gaaacatcgg atttcgccgc
    10741 tgacgcggtt ccccctggaa cttgtagcaa aacccattat aagtcccgta ggagtgatgt
    10801 caaggaacgt acatggcggt tcgcgacgtg tgcttcgaaa caattaagag tggaatgtga
    10861 cccagggccg attcagcgag ccacttttcg cttgattctg tagcgcccgc cttcaggcgg
    10921 gcatctgatg gactctacaa acacccgcct gaaggcgggc gctacgcaga ccgaacttct
    10981 tttgcgccga gtagttcggc gtgcaggcga agcaagtagt gatcggtcat tcctgcaatg
    11041 taatcgcaga cgacgcgata cttgtcgcct tgtttcgcaa gatcggcgta ggttttgggc
    11101 attgcttcgg gatgttccac atagaaatta aaaagttgtt cgagagcatc taccgaccgt
    11161 tcgcggtcca cgacgatggc aggctggcta tacagtctag cgatcagaaa ctttttaagc
    11221 gcaccatttt ccgccgccat ggttgcgctg aatccagcaa agcggcgcgg cgcatgacgg
    11281 acatcttcca cggttttcac tccggaaaca gtgatcgcgg tttgcgtgtt ttcgatcaag
    11341 ccagtcgcga gataattgag tacgcgcttg gcggcttcat tgaattttaa tttttcgagg
    11401 ccctgcggat attcgcgttc gacctgccgg taaaaccgcg cgaatagcgg aacttccgtg
    11461 caaatcattt cgatatttaa aatgtgagct tcgtatccgt cgtcgagatc ggctgtgttg
    11521 tacgcgatct catcgacgaa atcgatcagc tgcgcttcga tcggagggcg ctggtccagc
    11581 agatattcag aaagcgcggg aaattgcgcc gcgtcataat cgcgggaatg tttcacgatg
    11641 ccttcgcgaa cttcaaacgt gagattcaag cctggaaaat ccagatagcg ctgctcgaat
    11701 tgctcgacaa ggtgcagcgc ctgcaaattg tgattgaagc tttccccatg cgggcgcagc
    11761 aattcatcca gtttgtgttc ccccgcatgg ccaaaaggcg gatgcccaat gtcatgcaca
    11821 agcgccaggg cttcggcgag atcggaattc agttgcagcg ctccggcaac ggtgcgcgcg
    11881 acctgcgcga cttccagcgt gtgcgtcagg cgattgcgaa aatggtctga gtaccgggtg
    11941 gtgaaaacct gcgtctttgc ttcgaggcgg cgaaacgcgc gcgaatgtat gactcgatcc
    12001 cgatcacgtg cgtaatcatt acggtaagga tgctgcggct ccggataacg cctgccgcgg
    12061 gatttcgcca cgtccatcgc atacgaggcc agtgccatag catgattcta tgctactgcg
    12121 aggggcattc caaacaacgg gcggctgaat ggatgtttag cgttgagcga gccgcgaatt
    12181 tttgtctggc ccggtgtata gtatttgcca agtaataatt ccgttcggat gaatggatca
    12241 tcgaagcgca ggtatgccta tgaaaataaa actgctgatg gcgtttattc ttgtcctctg
    12301 cgggtgcgtg attgcggctc ctctcttcgc tcaccattcc agttcggcgt atgacatgtc
    12361 gcatcctgtc gacatgaaag gcgtcgtgac aaacatggaa tggtccaatc ctcacgtctt
    12421 tatttacatg aacatgaaaa acgataaggg cgaggtcgaa gagtggcgcg tggagggcaa
    12481 cagccccaac atgctttttc gcgcgggctg gaaaaaggaa atgatcaaag caggcgatga
    12541 actcagcgtg aacggcgctc ccgcaaagaa tggcgcaaaa tccctgcgcc tgatcagcct
    12601 cactctggcg aacggacaaa aatacgacgg ccagggattt aaatagtcac atttgaattt
    12661 gaaatttaaa gtggagccca gagtgcgaaa ttgttccgtg gttttattaa gtgcagcgtg
    12721 cattctcgtt ttgccgccgg caatttccgc gcaagcaaag cccccagcgg cgcccgcaaa
    12781 aacgcattct gcttccgcga aaccagattt atcaggcgtt tggttcatcg atgagtatca
    12841 ccgaaatctt cttccgaacg aagatccccc gtttcaacca tgggccaagg atttattcaa
    12901 gaagcgccag gaagaaagcg cgaaccgcac tccggattcc ggacctgacc caacggaaaa
    12961 atgcattccg ccgggaactc cgcgcgaaat gtttcaaccc tttccgtggg aaattgttta
    13021 tgcgcgggat aggattctca tgcttttcga gtaccaagga ttgatccggc aaatttttac
    13081 cgacgggcga ggacatccga aagatttaga gccaacgtac atgggtcatt caattggaaa
    13141 atttgaagga aacacgctgg tcgtcgacac aattggtttc aatgataaaa cgtggctcga
    13201 tccgatggga ttgccgcatt ccgatgcgat gcatcttacg gaacgtattc gccgcgtgaa
    13261 ccatgacaca ttggtggatg aatacacgat tgacgatccc aaggcctaca ccaaaccgtg
    13321 gaaagcgcag cgcaccttcg tgcttaagcc agattggcaa atacaggaat acgtttgcgc
    13381 ggaaaataat aacgtgaagt gaaattttga aattcagtag tgcgggcttc actggcagct
    13441 gtagcccgcc tcttcagagg cgggggttta gatggaacgc caaatatccg tcgtgtggca
    13501 tttaaatccc gcctctgaag aggcgggcta cagcagcatt taaaactcta gtcaggattt
    13561 tgcgttgagc agttccagcc cctgatccgc ttccgcggct gccgccggat attcctgctt
    13621 cacctgatta taaagtttgg tggcctgcgc gggatcgctc ttgcgataat aatccgcgag
    13681 cgccagcatg gacatggctt tgggaaccat gatgctcggc ttatcggata gctgcttcaa
    13741 aagatccacg gcctgcgagc ctttattttc ctgctggtaa acctgggcca tttgatattt
    13801 ggcgaggctc gcaagatttt catcgccgcc cgaaatcacc tgcgacagat ttttttccgc
    13861 gtccgcataa tttttcaatt gaacttcgga aagcgccgcg tagtattccg ccatctgtcc
    13921 gggtttcgta cgtccaaaat ccgccgcaac ggctcgaaat ttcttttccg catccgtgaa
    13981 tttattcttc tcgtcgaggt aggtaatttc cgtcggatcc gccggttccc ccgctgcacg
    14041 aatgcgcgcc tggtaaatct tcagcgcatc atccagcgcc agggaagcct tcgccgtttg
    14101 ccgccgggaa tagctgtccc aacccaatac cgccagcgcc acgaccagcg ccgctataat
    14161 cacaatccac attgcctgct gatgcgtagc gaccgtttca aagcccgaga ccatcttctc
    14221 ccggatttcg tccttcttca ggtccctgcg caaaatatgt tgcgccacag atttaaccct
    14281 cccactgatt taagaatcat taatggtatc acaggtttaa ccatacagtg gaagacagaa
    14341 ccaatccgtc tagcacctga actcactgaa gaggggttag gtttcgtcgt ggccggtcca
    14401 gttgcaccag accgggcacg gtttagatga atgttagcta aaaaatgttc ccgtcaggat
    14461 acaatcccaa aaatcgccgg ttcggcaaat tacgccgaac cgcctctacg aaagcgtctt
    14521 accaaatgga ttttcgccga aaaaatattc ggctgcatcc gacacgctat aaggggaacg
    14581 gctggtactt cataacgata tgttgtgagg cacgtaaatg tatttttccc gaaccgaagc
    14641 agggcgataa aatcatcgac ctaatcaaaa tcgcttccga gaaatacaaa tttggcgttc
    14701 acgcatattg tgtaatgccc gatcattttc acgtgctggt tgaaggcctt gcctcggata
    14761 gcgacctcct tttatttgtc cggagtttca agcagtcgtc tacacgcgca ttttcaggag
    14821 gaaatggcat tcctctgtgg caaaagaaat tctacgatca tattttgcgc gcggaagaat
    14881 cgcctgaagc cgtggcctgg tatatctgga tgaatccggt ccggaaagga ttttgtagca
    14941 atcctgacga atatctctat tctggatcgc tcactacgga ctggaagaaa atggtgcgga
    15001 ctccggagag ctgggtaccg gcgtggaaac aaacccaagt cgcccgtcca taattcggac
    15061 ggcctctacg agggccaaac caattcacaa aaagcgaaag ggccctgcct tggtttgctt
    15121 ggcagggccc tcagaacgct tggttccgac taggaagcag cagttacgct ttcagcctga
    15181 tagcccttcg gtccttgttt gacctcgaat tcgaccatct ggccttcgtt cagtgtcttg
    15241 tatccatcca tctgaatggc ggagaagtgt acgaacacgt cttcacccga ctggcgttgg
    15301 atgaatccat agccttttga tgcgttaaac cacttcacta caccttgttc tctgctcacc
    15361 ttcttactcc tctatcgcga gcgctcgctg tttgctgggc gtaacgccag tcggtgatac
    15421 ataaatgtcc gcgtgaccgg tgacccttcc ccgtccgtcc tgaaaacctg acgagagcgg
    15481 aggaccgtag cgggccttcg aacacctacg gaattactag tcccacaaaa gcgcgccatg
    15541 aatacaacaa aatttcatcg ctgtctagag gtttcccatt acgggaaatc tccggaaatg
    15601 ggaggtcgtc agccatacgg tcggctcaaa attccgggaa atctgagttg tgcctgtgaa
    15661 tgacgcggga ggaaagaaac tacgattgaa aacttgccaa cgcttgttcc tcttgattgt
    15721 gaatatcgaa taccgtgtaa agcttggtga tttgcaacag gtccgtgact tttttattca
    15781 aatttaatag ctttacttcc ccgccttcac ttttgatcaa gcgatagcca ctgaccagtt
    15841 caccgagccc ggagctatca atgtaattga ttccggcaag attcatcact atttttttat
    15901 ggcccttggc aaggttttcg tgaataagat cacgcagcag ggcgctgccc tctcccagcg
    15961 tgatgcgtcc gtccatgtcg attaccgtaa cgcttccgat ttcacggctg gtggctttaa
    16021 aactcacgtg aacacctttc cccgaaatat ttcttgcctg aagaaaaacc gcgcaggccg
    16081 cgcttccctg cgagcatcct agtgcttgtg gtgtttggcc attacgactt ccgcgccgcc
    16141 cgctgcgctg gagaccacat ccacttcatc catgaaggcc ctcatcaaca gcacgcctcg
    16201 cccggaatcg ctcagcacat tttcatcgtg gcgtggatcg ggcacatcct caagccgaaa
    16261 tccagccccc tggtccacca catgaatcac gatcttctct tcatgcaatt caaatgtcag
    16321 gtggattttt ctttcgcggc gctcttgatt gccgtattgg aaggcgttca taacgccttc
    16381 gtgcactgcg agaccaattt tatattggtc atcctcgcca aatcccgctg ccgcagccgc
    16441 gcgaacgccc atctcctcgg cgagggcaat actctcgatc agtgtttcta gaacaacttc
    16501 aatccgctga ggtttcgagg gcatccggct gttggattcc gccttgggac ttgtatgggt
    16561 aacatagcgc aaacgcttaa gtcaatccaa tacatgatct tggaagaaca tggatggtag
    16621 cgctttgtag cggttccagc gggcaaaaca atgctaaatt tgaaagttcg gggtgctcat
    16681 gaattcgtat ctactgctca gcatcgctgg tcttgccgcc ggactcatca actcagttgc
    16741 tggaggcggc atgtttatta cgtttcccgc attggttttt actggagtgc cttcgattat
    16801 tgccaatgct tcgagcacca tcgcgctgat tcccggagtt ttggcgagcg ctggagccta
    16861 tcgtcacgat ttccgcaaat cggaaaattt cccttttatt cctttgctgg tagtgagcgt
    16921 ggcgggcggc attgtgggag cgctgctttt gctgttcacg ccgcaaagca cgttcgactc
    16981 tgtcattccc tggctattgc tcgtggcgac actgctcatc actttcgggc cgcgtatttc
    17041 tcccttgctg aagcgtgtaa ttcatatagg acccgtggcg gtcgttgtga ttcagttttt
    17101 tattgcgatt tacggaggct attttggcgg agcgatcggc atcttgatgc tggcgacgtg
    17161 gacagtcttc ggccttacag acatccacgt aatgaacgca aacagaacat tgatgggagc
    17221 tgcggcgaac agcatcgccg cggttttatt tatcgtggcg cacaaagtgt ggtggccgca
    17281 gacgctcgtg atgctggtat tttgcagcat cggcgggtac atcggggcgg ttgcggcaaa
    17341 gaaagtacac cccagctatg tgcgcgtttt cgtgatcttg atcagcacaa gcatcacgat
    17401 cgcgttcttc ctgcgaaagc actaagtttt aagtaacgca ggtttcagct ggtggaattt
    17461 aggcctagga agtcctgact cagcaaatta aagtccgcac taaaaataat cagattaaaa
    17521 aataattgat cccggtacat attcggtgcc ggctcttatt ttagaaaact tcaaagtttc
    17581 aatgcggatt aagattataa taaacaatag tttaagaacg ccttataagc cgggatagta
    17641 ttgggacgaa gcggaaagga aatcggatgt ccaagatcga tgagctgaaa gccgagatcg
    17701 aaaatctttc gagcaacgaa gttgcggaaa tttatcgctg gttgtcggaa aaagactggg
    17761 aaagatggga taaggaaatc gaagacgata gccagtcgca aaaaattgac ttcctattgc
    17821 gagaggctca tgaagaaaag gttaaaggaa atctgaaaga cctataaatg catcacgcca
    17881 ccagccgctt ctggaattca tttggcctgc tgccggaaaa cattcaggaa attgcccgca
    17941 agaacttttc cctattgaaa aacgacccgt cacatccgtc actacatttc aaaaaagtag
    18001 gaaagttatg gtctgcaaga gttggaactc atcatcgtgc ccttgccgtc ccggatcgtg
    18061 cggattttct ctgggtctgg atcggcactc atgatgagta catgcgccta atcaagcaag
    18121 aaggttagag ctacgaagct ttctgcttcg cgtaaaccag aaaatcctcg tacggtccct
    18181 tgaagtcctc gacgtgcccg cccttgcact gccaaatacg cgtggcgact tcgtcaatca
    18241 cgtcatggtc gtgcgtaacg aagatcacgg tgccgggaaa cttctgcaga gctatgttga
    18301 gcgcgttgat ggattccaaa tccagatggt tcgtcggctc gtcaaacacc agcacattcg
    18361 gtttctgcaa catcagcttg cagaagatca agcgagcttg ttcgcctccg gaaagagcgc
    18421 gcgtggattt ctcggcgtcc tcgccgctga acagcatctg gcccagcacg gcgcgcaatt
    18481 cctgttgcga agccttggga tcgaactggt gcagccattc gatgacattt gtattcttgg
    18541 cgattgtgtc ggtggtgtcc tgcgcgaaat aaccgaccac cgcttcatga ccccacttga
    18601 cgtggccgcc gtccacggga aattgacggt cggaatcttc gatgtcgccg accgcactgc
    18661 gaaccagcga cttaatcaaa gttgttttgc cggctccgtt tcggccgatg atcgcgattt
    18721 tttctccgcg ctcgacgttg gccgtgaatt tgtcgatcac tttatggccg tcataggtct
    18781 tcgtgaggct cttgaactcc agcggatgcc gcccggacgg aattttcatg tcaaagcgaa
    18841 tgtagggccg cgcaatattg gaccgcgcca gctccgaagt ctgaatgcgc tcgacttctt
    18901 tcttgcgcga agtaacttgc gaggaacgcg taccagccga aaaacgcgcg atgaattcat
    18961 tcaattgcgc gattttcttt tcgcgcacgg cattgtcggc ttcgatgcgc gagcggattt
    19021 gcgtcttggc caaaaccatt tcgtcgtagc cgcccgtgta catgatcacc gtttgataat
    19081 cgatgtccgc ggtatgcgtg cagacgctgt tcaggaaatg acggtcgtgc gagatgacga
    19141 tcagcacgcc ctgatattcg tgaagataat cctgtaacca gtgcacggaa tcgaggtcca
    19201 agtggttggt cggctcgtcc agcagcagcg ccgcgggatt tccgaagagt gcttgcgcca
    19261 gcaatacgcg cactttctgc ccgccctgca actcgctcat gttgcgctcg tgcaaagcag
    19321 cttcgacgcc cagcccgtcg agcaggatcg cagcgtccgc ttccgccgta tatccgccct
    19381 catcgcccac tacgccttcc agttcgccaa ggcgcatgcc gtccttgtcc gtaagctctt
    19441 cgtgaggctt ggcgtacaga atgtcccgct cttgcagggc cttccacaat gggccattgc
    19501 ccatgatgac tgtgtcgatc actcgaaact gatcaaaggc aaactggtcc tgccgcaaaa
    19561 ttccaagttt tttggggcgc gaaacggaac ccgttgtcgg ttccagatcg ccttccagaa
    19621 tcttcatcaa agtggttttt ccagcgccgt tcgggccgga gattgcatag cggcgccctt
    19681 cgaggaacgt gcaggtaacc tcctcgaata gcagcctcgc gccatagcgc atagtgatgt
    19741 tatttaacga aagcatatct ctccattgta tcttgaaatc ggcaagcgtg cttgttgatc
    19801 agggccgcgg ggcgtattct gaatcacaac ggaagcgctt gaaggccgag gcaacagttt
    19861 agactacgtg acagtatgat tcaattatga aaaaccaact atttgcggtc atttttgcga
    19921 tccttgccgg cttctgttcc tgtcgaatgg cacctgcaga tcgtgccgct cgatcgacag
    19981 cgtgcgccgg aagatattcg ccacaaataa ccgcaaaggc tgaccgttaa tcgaaaaaat
    20041 atattattct gtcattctaa gcgaagcgag gaatctctct tagatttcaa cggaacaaga
    20101 aaaccaaaga gagattcctc gcttcgctcg gaatgacaga aggcgatatg gccataacaa
    20161 gcggcatgtg gaagaaatca tgaaaatgtc tcgcgtaatt ttagggctta cccttctgac
    20221 ggttgcttcc gttaatgctg cgccgcagca gcaaaaaaaa gtcgccgccg agcccccagc
    20281 ctgggcttac ccgatcaatc ctcccgatga tcgccccgcc gcggatgacg gttcgcttca
    20341 tcacgtgccc ggcagtagca aaggcctaac gctcacacaa atcacagact ggttcaatat
    20401 tccggactgg catcctgagg ggcatccagc gatgccggaa attatcgcgc acggaaggaa
    20461 accagccgtg cgcggatgcg gctactgcca cctccccaat gcgcagggtc gccccgaaaa
    20521 tgccagtctt gcggggcttc ccgcttccta tatcgttcag caggtcatgg aaattaaatc
    20581 cggcgtgcgc cgatgttccg aaccgagaat ggggccacca ggaaatatgg tgtcgattgc
    20641 gcaaagcgct tcgctggagg atgcaaaagc tgccgccgaa tatttttcct cgctgcaatt
    20701 gaaaccgtgg atcaaagtga ttgaaacaga ctcggtgatg aaaactaagg tgaagggcag
    20761 catgctggtc gcggcggatg gtgggggcaa agagcccatc ggccaccgaa ttgtcgaaac
    20821 gccagcgaat ctggagctaa cggaattgcg tgacgatgcc tctggattca ttgcatatgt
    20881 tccggtcggg tccatcgaca agggtaagac actcgtcatg gccggcggcg aaggaaaaac
    20941 aacccgatgc tggttctgcc atggtcctga attgcgcggc cttggccctg taccgcggct
    21001 tgcaggacgt tcgccaagct atctctttcg gcagctctac gattttcaga ctggcaaccg
    21061 ccacggcgaa tggagcgcac tgatgaagga tccggtcgcg cacttgacca atgacgacat
    21121 gctgaatatc gccgcatatc ttgcttcgct ggagccgtag cctgtaaaaa aaacgaaagg
    21181 atttaacaca gaggacacgg agaaacgcac agagaacacc ggagaatgat gtggaaattc
    21241 aaaaatttct ccggttttct ccaagatttt tctctgcgtc ctctgtgtta aatccttttc
    21301 cggcgcccta tgcccagtag gaccgatcca gcgttcggta ttgaatagcc tcaccaaggt
    21361 gccgcgtctc aaggttttcc gacccgtcga gatccgcaat cgtgcgcgca actttcagga
    21421 tgcgatcgtg tgcgcgcgcc gaaagcccga gacgccctat cgcattctcc agcatttttt
    21481 cgccctcctc actgatcgcg caaaatttcc gaatcaactt tggcggcatg tgcgcgttcg
    21541 agaaaacttt tcgttctcct gcatagcgct tggattgccg ctctcgcgcc cgcagcactc
    21601 gttcgcgaac cttggcggag tcctcgatgt ttccgttgcc gcggagttcc ttatatttca
    21661 cggcgggaac ttcaatgtga atgtcgatac gatcgatcag cgggcccgaa atttttgaga
    21721 cgtaacgctg aatctgcggc ggcgagcagt ggcactgccg cgtagggtcg ccgaaaaatc
    21781 cgcacgggca tggattcatg gcggcagcaa gcatgaagcg cgcgggaaaa gtgatcgaag
    21841 tcgccgcgcg cgaaatcgtg accgcccctt cttcgagggg ttgaggcatc acttccagca
    21901 cgttgcgctc gaattccggc aattcgtcaa gaaataacac gccgtggtgc gccagcgaaa
    21961 cttcacccgg ccgcggaatc gctcctccac cgatcagacc cgcatcgctg atcgtgtggt
    22021 gcggcgaacg gaacggccgc gtcccgacaa gcccgcgcgc atcgtccagc aatccagcga
    22081 cgctgtgaat tcgcgttgtc tcgattgctt cttccagtga catggaaggc aaaatcgtgg
    22141 gaatacgttt cgcgagcatc gtcttccccg cgcccggcgg cccgataaaa ataatgttgt
    22201 gcccgccagc gcaagcgact tcgagcgcgc gcttcgccgc ctgttgccca cgtacgtctc
    22261 gcatatcgac agcatattgc gaagcatcgg ccagcatcga gct
//
LOCUS       DASZMB010000213        25572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4067127, whole genome shotgun sequence.
ACCESSION   DASZMB010000213 DASZMB010000000
VERSION     DASZMB010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 25572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25572
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4067127"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VG272_09960"
     CDS             <1..214
                     /locus_tag="VG272_09960"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF014067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HWF14047.1"
                     /translation="QFELSLDPATAKAMHDETLPDDFYKDAKFCSMCGPKFCSMNVTQ
                     LAESAGGQDQSERQQKFVELLAKIEK"
     gene            complement(243..1577)
                     /locus_tag="VG272_09965"
     CDS             complement(243..1577)
                     /locus_tag="VG272_09965"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF14048.1"
                     /translation="MDTVIPSRTNIAERTVRHVNRRLLPYLVILYVVAYLDRVNIGSA
                     GLQMTRELGFSNAVFGLGGGIFYLGYMLLEIPGGVLAEVWSARKWIARILLTWGFLAS
                     ATGLIHSTKEFYWLRFLLGLAEAGFLPAILVYISHWYRPEDRGKAIAIFMASNPAAQV
                     IGGPLSAIFLKIHWLGYNGWRWLLLLEGIPAIILGFMMLSYLTERPEEAQWLQKDERE
                     WLTSELEREREARASHIPAWRALVNPRVLLLSITLFLGLTATYGVSLWMPKIVEKVSS
                     FGVSKVSLIAAIPYLFALPVMWLAGWSSDRSGERRWHAAIPRIVAGVALAACIYSTDY
                     VWISVFALSIAAAGFYSSHAGFWPIPNMLLGRAAAATSIGLINTFGSLGGFVGPYVIG
                     YLRDKTGGFGSSLLFLSACSVASGLLVLCVRLPVARASACGGSSLQGQNSTG"
     gene            complement(1865..2887)
                     /locus_tag="VG272_09970"
     CDS             complement(1865..2887)
                     /locus_tag="VG272_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14049.1"
                     /translation="MKKYKLEYIWLDGRKPVPELRGKTTMKSFDSPPALADLPLWGFD
                     GSSTMQAEGKSSDCVLKPVAMYPDASRKDAYIVLSEVMNADGTPHPTNARATIINDPD
                     LWIGFEQEYFLFKDGRPLGFPAEGFPGPQGPYYCGVGYKYMGSVARQIVEEHLELCLA
                     AGINHEGINAEVAKGQWEFQIFAKGSAKAADDVWIARYLMMRLCEKYEVDVVLYCKPI
                     KGDWNGSGMHTNFSTKYMRETGGKEYFESLMKAFEKHIDAHIAVYGPDNHLRLTGLHE
                     TQAIDKFSFGLSDRGASIRMPINFIKNGYKGYLEDRRPNSEGDPYQIVSQILKTINTV
                     PKPVLV"
     gene            3163..3882
                     /gene="ispD"
                     /locus_tag="VG272_09975"
     CDS             3163..3882
                     /gene="ispD"
                     /locus_tag="VG272_09975"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HWF14050.1"
                     /translation="MMNRVTAILPAAGLGTRMGAETPKQFLSLDGVPVLLFTLRRLAA
                     CPAITEFIIATRAEEVESLAASVSKEKFARPVRVVRGGDSRQDSVANALTHVALETDL
                     ILVHDAVRPLVTVSQIERVIAEAAACGAAILGIPAMDTVKEVKRASLPNDVALITATI
                     PRERVVLAQTPQVFRATLLIKAFGQARQDGVTASDEAGLVERAGHDVHVVVGSERNLK
                     ITRPGDMELAEFYLRQERAGR"
     gene            3879..4352
                     /gene="ispF"
                     /locus_tag="VG272_09980"
     CDS             3879..4352
                     /gene="ispF"
                     /locus_tag="VG272_09980"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HWF14051.1"
                     /translation="MSRCGIGYDLHRLAEGRKLILGGIELAFPKGPVGHSDGDALAHA
                     MCDALLGAAGMGDIGTHFPDTDPKWKDAQSLRFLEHIRDLLAEHKFRIVHIDATVITE
                     KPKLGPHFPAMREALAKSLGISPSMINLKAKTNESVDAIGRGEAIAAQAIATLDR"
     gene            4477..5238
                     /gene="rlmB"
                     /locus_tag="VG272_09985"
     CDS             4477..5238
                     /gene="rlmB"
                     /locus_tag="VG272_09985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HWF14052.1"
                     /translation="MDRLTGIHAVREALEANRPLDSLLVAKGTHGNRVEELVRLARKL
                     DVPVRFEERAQVDRSAGTREHQGVVAFVASTPAMNFYDLLKPRPEGSAPGLLVLLDGV
                     EDPQNLGAIVRTSLAAGAQGVVIPERRAAGLTEAVARASAGALAHLPVARVTNLSRAM
                     EEMKESGYWLVGLDERAEKLHTDVDFKVPVGVVMGGEGKGLHALVRERCDFLVSIPTT
                     GPVRALNVSVAAGVVLFEVVRQRGKTGKQPRMHTD"
     gene            complement(5398..5919)
                     /locus_tag="VG272_09990"
     CDS             complement(5398..5919)
                     /locus_tag="VG272_09990"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002634.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HWF14053.1"
                     /translation="MNHLKEFIREVPDYPKPGILFYDLTTLLKNPGAFHSLVDNLCEH
                     YAGQKVDAVVGIEARGFILAPALAYRLNAGFVPVRKPKKLPAKAVSVTYQLEYGTDTL
                     EIHEDAIKPGQSVLLSDDLLATGGTAAATVELVRKLGGKVVGASFAVELTFLNGRAKL
                     PGIDVFTMIQYDK"
     gene            complement(5996..6283)
                     /locus_tag="VG272_09995"
     CDS             complement(5996..6283)
                     /locus_tag="VG272_09995"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HWF14054.1"
                     /translation="METLAKRFFVSGMVQGVGFRFFVERTAATVGVNGYVKNLFDGRV
                     EVYAIGSAEQLDALRNALRRGPRMASVENVEELDADILREYANNFSIENDE"
     gene            6588..7454
                     /locus_tag="VG272_10000"
     CDS             6588..7454
                     /locus_tag="VG272_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14055.1"
                     /translation="MNQGFKNWRMLFALAMLPLAAPAALHAQATGPMQPPASTKFPTP
                     PPPSKPDTPALAPEEIIRRFAAKEDEMIRAIRGYTFQKSVRVEEIGPGNKPAGQLEIV
                     TEQRISPEGKLFEKPVSRQQSTLQYLDLQRGDSDLVAQAPMFPLSTAMLPKYEIVYGG
                     KQPLDDLSVYYFTIKPRALERAHAYFSGVVWVDEQDFVIVKTMGKWVTETGDVTSSNL
                     PFTVFETYRQQVGKNLWFPAYSRSDETFQSNNVNVPIRMIIKWTNFAPQTSTPAADSP
                     AMGNPPSNQAHP"
     gene            complement(7464..8447)
                     /locus_tag="VG272_10005"
     CDS             complement(7464..8447)
                     /locus_tag="VG272_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWF14056.1"
                     /translation="MNSASQTSLEKLPEVNIRLLREEDLPAADRIMRLAFGTFLGLPD
                     PLKFMGDAEYVRTRWPVDPSAAFAADLSGELVGTNFATRWGSFGFFGPLTIRPDLWDR
                     GIAQKLLAPTMNLFDAWGVTHAGLFTFASSTKHVNLYQKFGFWPRFLTSLMSLPIDEN
                     PSAGRRKWLRFSDLSESEKEECLQACRELTNSIYEGLEVDREIRAVDSQTLGDTVLTW
                     DSGKLAGFAVCHVGAKTEAGSGKCYIKFGAVQSGQDGALLFRRLLDACREFAQSRGTK
                     SLVAGVNLARADAYREMLQAGFRATAQGVAMERNGDAGYNRHDVFVMDDWR"
     gene            complement(8464..9504)
                     /locus_tag="VG272_10010"
     CDS             complement(8464..9504)
                     /locus_tag="VG272_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14057.1"
                     /translation="MELKLRKNAFSFPYSFFLLSVFICVHLWFLPPVARAQEAQDQEV
                     AVNLAEGRVVICAAKDGIILAAMNAHGEATSKPPAIEVLSADRMGVMLGAVEWERPDS
                     KEDTIRLDNEFRRIIAAAFNSSGKPKEADAGNDIETIGIALLERIRQVAELLRHKVNL
                     GDDEPLIRIVLSGYVRDYGPEVWTIDYHIRQDLLGNDIWRTRVLRPTYNQLYPPEKGK
                     PKTFMEVRYPPENRASGEAEFLDLLQQNDGRLTKIRTANEIQAKSVTLATEGQSQKAD
                     AASLINFMKAAFPAVTSPETKLTMAMVDYDEGFKWIIQPPKAPAPPPGEKPKDKTPEE
                     PERPTLRRSGGS"
     gene            complement(9587..10630)
                     /gene="pta"
                     /locus_tag="VG272_10015"
     CDS             complement(9587..10630)
                     /gene="pta"
                     /locus_tag="VG272_10015"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF007233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HWF14058.1"
                     /translation="MEKLKNRVRSHLQRIVLAEGEDPRVVAAAATVSREGFAKITLLG
                     RKQIIAAIASDMYADLAKVTIVDSATSERGEVYAQIYYERRRAKGASLQEASEIARRP
                     LYFAALSVAAGDADGSVGGAANTTGEAVRAALHTVGLASDAKLVSSFFVMLVPERAGL
                     DMSTGGAFLFADCAVVPDPNAEELAEIARAAAENARAILDAEPRVALLSFSTKGSASH
                     PRVEKIKEALRILKARAPQIAADGELQADTALLASVANSKAPGSPVAGHANVLIFPDL
                     DSGNIAYKLVERLGGAQAIGPILQGLDRPANDLSRGCSSEDIVNVVALTSLQAIARKE
                     KTLEPQMHADQHG"
     gene            complement(10664..11158)
                     /locus_tag="VG272_10020"
     CDS             complement(10664..11158)
                     /locus_tag="VG272_10020"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWF14059.1"
                     /translation="MENFNAIISALERSPEIILPLVREVPEEILKRRPKPGKWSAHEH
                     ACHLAAVHPLMSSRLDLMLAEPAPRIRPYLPHVDDEEDALLKTNLDEALQRFTRDRQR
                     LVDQLKTLSPEDWGRRAEHGEYTHYSVFIMFRHLAMHDMFHAYRIEELLLKNDWERLA
                     ADLA"
     gene            11283..11819
                     /locus_tag="VG272_10025"
     CDS             11283..11819
                     /locus_tag="VG272_10025"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HWF14060.1"
                     /translation="MPRKADTIRHKGKKLEDILVAHEKFHKGHPGGERADLSGANLSG
                     QDLKKRDLTGANLVGSNLQKADLRGCKLSFADLSKANLRKADLRNCDFTETKLEGADL
                     SEANLSGTELFRGDLRSAKLHKTILRGTVLRQANLRGADFSGSDLALASFREIDLTDV
                     KMDGADLKDAVVRDNRKS"
     gene            complement(11854..13335)
                     /locus_tag="VG272_10030"
     CDS             complement(11854..13335)
                     /locus_tag="VG272_10030"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HWF14061.1"
                     /translation="MSKGSILVVDDESEIREGLELLLNVEGYSTSSADSAETGLAMLD
                     EKPFDLVLLDVSLPDRNGLDVLREIQRRSPGLAVVLITAYGSIDMARAAFKSGAQDYI
                     TKPWSNDELLVQVAQAVEGQRLREENVQLKRALKQRYNFPNIIGKSEKMLAVLDLVSQ
                     VAPSRSTVLIHGESGTGKELIAKAIHSASTRADKAFVPINTGSIPVDLLESQLFGHVK
                     GAFTSAVTSKKGLFEVADQGTIFFDEISTIGPETQAKLLRVIQEREFMRLGGTETIKV
                     DVRIIAACNVQLLNLVREGRFREDLYHRLNVIAVPLPPLRERKEDVPLLLDHFLNVFA
                     KENARPVGYFTQPALRLLMDYDWPGNIRELENVVERAVVLSSQEQLDVELLPESVRTK
                     ELLGGVRLHLSEFAPPLPGEHGSRSGADSAKNSLFQIMDEIERRVIVGMLERTDWNQT
                     EAAESFQIPLSTLNQKIKRLGIDVRRRGITRNASESSASATGR"
     gene            complement(13328..16165)
                     /locus_tag="VG272_10035"
     CDS             complement(13328..16165)
                     /locus_tag="VG272_10035"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF14062.1"
                     /translation="MSAPFNDSLRVRLGAAVLALATLAAIVFGIINFQQRTLFTAPDD
                     GVSWIANPSGVVAWHVTPKSPGEVAGLRTGDQVLAINDVPIRGPIDVTKRLWRIGLWS
                     LAHYQIERGGKKFEVQLVIEPTARPSSIENYLSVVGVLYLFIGLFIFARRWNATRAVH
                     FYIFCLVSFVLYSFQYTGKLTTFDWEIYWAAIVARLLQPALLLHFAMVFPERRPASQG
                     RGTILAAIYGVPGILLIVRILVGLRELGFMPSVEARNTLDRLDLACLGIYFSLAALVF
                     VTSYRRASSGILRQQLKWVTGGALAGIVPFLLLYIVPFFLGVVPRPWMNFSTISLVLI
                     PLSFGYAIIRYRLMDVDIIFKRGLAYTAASGGVVAVYIAIVALIGALFHTAWPSGTMG
                     GVIAIVVAAFLFQPFRDYIQARLDRFFYRDRLDYRRTLIEFGRTLTSEVRLDPMLASV
                     MDRISQALLVDRLAIFIEDESRPGTYRIARSMGVRYSGALDMSFLDEECKPFAQGVLF
                     YESARAARESSPPVRYTLEQLDLNYFIPCQIRDHAIAVLGLGKTVDGDFLSSEDVELL
                     HTIAGYLAIALDNAQLYNSLEQKAAQIERLKDFSENIVESLNVGVLAVDFNGAVESWN
                     TQLEGLIGVPRQEAVRRKLEDVLPADLMAEIAARSADERVSSLYKFPMRSRDGRNLVV
                     NVSIAPLVGKSGNRIGRLILFDDVTQRMRLEEQVFQNEKLTSLGLLAAGVAHEVNTPL
                     AVISNYIQMLAKQLPVSDPRQQLIEKVVKQTFRASEIVNNLLNFSRTGAAEFTEVNLN
                     AVVEEVLTLVAHPFRTAQVQVTRNLQQELPAVLGSNNKLQQVFLNLFLNARDAMPSGG
                     VVEIRTAAHNGSVEVEIIDSGGGIPRENLNRIFDPFFTTKSSGRGTGLGLSVSYGIVK
                     EHAGKVDVRSTPGKGTSFRLEFPVARKAVHV"
     gene            complement(16358..18064)
                     /locus_tag="VG272_10040"
     CDS             complement(16358..18064)
                     /locus_tag="VG272_10040"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWF14063.1"
                     /translation="MQSIFRTLGWVIVVTLGMAASTAAKPGYGTISGVVLDSSGTPQM
                     GASVWLISEDGGRIVAQILSNQRGTFITDHLKPGAYDVRVSMAGFLPAMEHHVAVMSN
                     LTTLLRVQVDTVFSSLDTLRRKPDATSEQDDWKWVLRSSAATRTILQWDDTSTTQTAS
                     AAIGGDLPSAQRPRALVQVGNGAMRPGSASNFPNAPATAVSYDQKIGKLGRMLVAGQM
                     SYDKGASSSFAGVWLPSGAGNGPETIFIWRQSKIGAEGMEFQGMRLDHTEQIELGDHV
                     QLKVGAEYLRAGIISATSSLRPHLQLNTTVSPDLTLSLIVASNPPSEQWGRSGTLESA
                     IDELNSLPPVLFHSGRPVLEAGWHQELSAKRRVGKQSAIEVAAFHDSSRDQAIFGTGA
                     SVSPEFVQDEFSSAFLYDGGNSSSWGTRVAYRQKISDAWEVATIYSWAGALSPGSAAD
                     LSPSAIRNNFTTSNHHSVAARISGKISQSGTQFSASYKWVAGSALSRMDQFGEAAYQM
                     DPNLHISIRQQLPGLNGRWEALADFSNLLAQGYVSVSGQDPRMILAPILRSFRGGVSF
                     QF"
     gene            complement(18074..19816)
                     /locus_tag="VG272_10045"
     CDS             complement(18074..19816)
                     /locus_tag="VG272_10045"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HWF14064.1"
                     /translation="MNRLERMSVILLIGGAFLLPASPRLHAQDQDKDQSAASADKQQD
                     SGKKKNDGKKNLKKLQKELATPYKKWLEEEVPYIITDEERSAFLQLQTNEEREQFIEG
                     FWQRRDPTPDTVENEFKEEHYRRIAYANERFSSGIPGWRTDRGRIYIMWGPPDSIESH
                     SAGSTYDRPMTEGGGQTTVYGFDDWNYHYMEGIGQNITIEFVDPTGTGEYHITMDPGE
                     KDAMAHVPGGGPSLLESMGMSTQAQRFTQSNGSTAPLGSAQVNGGGGLESQNEFTRLE
                     QFAKVQAPPPVKFKDLEEVVSSRILRNQISFDYRFDFMRITGDTILVPVTVQIQNKQM
                     TFRDHDGVQSASLNLFGRVSSMTGRVIQTFEDVIQRDFPDSLLEPSLKGFSIYQKAIP
                     LRPGLYKLDLVIKDVNSGNVGVVNTRLAVSPIPDDKLEASSLILADQMEPVSSKDVGV
                     GQFVLGSTKVRPKLDAEFHADQALDIYLQFYNLKVDDKTHRNNVSLDFKITQNGQVVS
                     HEVKTGDQIQQSGEQITVQQIIAPKTLAPGKYKVEIQATDLVANQTVTRSADFSVTPP
                     AMLAKVATNGATGR"
     gene            20332..21747
                     /locus_tag="VG272_10050"
     CDS             20332..21747
                     /locus_tag="VG272_10050"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWF14065.1"
                     /translation="MNMWKKIGIGVGVAAVLAGGVWIALWQINKGVATVQTAPALRQD
                     LNSIVTASGEVKPLTYTNVLGEGIGKITEIAVKEGDHVKKGDVLLRLESVQPAADVNA
                     QRANIAAAEAGIKSAAANDVSSQADIKSRAADLEHAKLDWDRGQLLFKDGLIPKQDYD
                     TRKASYDSAVAALASAQARAEQARAQLNQARSGLTQNQAMLGRLSDILHKTTYTAPIN
                     GVVTYIAVRVGENVVPGIQNATGSYLMTISDMSVVTSEVMVDETDIVSVKLGEDAEVT
                     IDAIPGKFFKGKVTEVGTQAVLRSSGLASTQSTTGNQEAKDFKVVVTLQNPPDGLRPG
                     LSSTAKITTAQKKNVVTIPIQALAERTKKDLDEAAKENGQNYVTLAAERPAPGPDGSG
                     STSALEKNPEIQGVFVVRNKRAQFVAVATGITGITDIEITSGLQEGDQIITGSYKTLR
                     TLKPDARVKVDNSTPKAPEDN"
     gene            21781..22521
                     /locus_tag="VG272_10055"
     CDS             21781..22521
                     /locus_tag="VG272_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF14066.1"
                     /translation="MAERAVSNETFRQDLVDQGVAILTEGLWKTYEMGGEKLHALRGV
                     DLTIRRGEYVAIMGPSGSGKSTLMNLIGCLDTPTSGKYWLAGRLVSELDDDELAAIRN
                     KEIGFVFQTFNLLPRATALHNVELPMVYNGTPAEERLARAKRALEAVDLGERMFHKPN
                     ELSGGQRQRVAVARALVNSPSIVLADEPTGNLDTKTGDEIMALFARLHSEGNTIILVT
                     HENDIARRANRIIHVRDGKVETDEPVAH"
     gene            22579..23688
                     /locus_tag="VG272_10060"
     CDS             22579..23688
                     /locus_tag="VG272_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWF14067.1"
                     /translation="MRSRISVFISIVQAILLLAHLFLYETWIFFHGTPDPPYGFALKI
                     VLSILSVSFVAASLLAQKYFNPLVRAIYTMAAVWLGLVNFFFLAACAIWIIYGLPLLL
                     GFHLEKRDLATQCFGAGLLAGILALANAAWPRVVRISIKLPNLPASWRGRTAALVSDL
                     HLGHVRNGGFVRRIVRKLSSLKPDVLFIPGDMFDGTAVNFGRLSKTWKEFSAPLGSYF
                     IAGNHEQFASYEKYFGAVTASGIRVVHNEKIILDGLQVVGVHYLDSKNMERFRSILAK
                     AELDPNIASVLLVHDPSLLSIAMEAGISLQLSGHTHRGQFFPFTKIVSRIYGIYAYGL
                     HHFDALQVYTSCGVGTWGPPMRLGSNPEIVLIHFE"
     gene            23916..24734
                     /locus_tag="VG272_10065"
     CDS             23916..24734
                     /locus_tag="VG272_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14068.1"
                     /translation="MPVQSISSLIQNLSDQLFGQAQDDNAQKSLLGLASPVTAAIAED
                     TFTPSTQSGIAQPAEQEAGLFQFAAGTLTTNQPGSTGRPAIGAPAISGAAQTAATTTT
                     AEASGASGQAATGTPATQAATSAAAAANIQLQIQSLNAALPALGLTNTEINQIDRIAS
                     LVQNFNPAAYANLVSQFEALAQNSGSPNSAAIPSTNSATPAANSQNGAGYQLQEISIK
                     FTGSTQPGSSSFAGGGGKNSDPNTPQSNSAGLQIGQVQFTLINGHGQTVRVQAL"
     gene            24929..>25572
                     /locus_tag="VG272_10070"
     CDS             24929..>25572
                     /locus_tag="VG272_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWF14069.1"
                     /translation="MTRALEEHRKSGKKLFDLTASNPTECGLAYPEQEILTALANPRA
                     LTYQPDSKGLLEAREAVAAYYAGRPGFSESPAPVDPERIILASGTSEAYTHIFCLLCE
                     DGDEILVPAPSYLLFEFLADLADIRLVPYPLLYDHGWQIDFASLRAALTSRSRAVLIV
                     HPNNPTGSFVKRHEATELAEICAAREMAIVADEVFLDYAENTGNRLTGSGSANL"
BASE COUNT         6124 a         7045 c         6687 g         5716 t
ORIGIN      
        1 gcagtttgag ttgtcgctcg atcccgcaac ggccaaagcg atgcacgacg aaactttgcc
       61 cgatgatttt tacaaagatg cgaagttctg ctcgatgtgc ggcccgaaat tctgctcgat
      121 gaacgtgacg cagctggcgg agtcggccgg cgggcaggat cagtccgagc gccagcaaaa
      181 atttgtggag ctgctggcga agatcgagaa gtaaaatcgc tgggcattac gtggcatagg
      241 cttcagcctg tggaattttg tccttgcaag gacgaacccc cacaggctga agcccgcgcc
      301 acgggtagac gcacacagag caccagcagg cctgaagcta ccgagcaagc ggaaagaaat
      361 agcaaactcg aaccaaatcc tccggtctta tcccgcaaat atccgatcac ataggggcca
      421 acaaaaccgc ccaggcttcc gaatgtgttg atgaggccga tgctagtggc tgcggctgcg
      481 cggcctagaa gcatgttggg aatgggccag aagccggcgt gcgagctata aaagcccgcg
      541 gccgcaatgg acaaagcgaa cacgctgatc caaacgtaat ccgtggaata aatgcatgcc
      601 gccagcgcaa cgcctgcaac aattcgcgga atagcggcgt gccatcttcg ctctccgctg
      661 cggtcagaac tccagcccgc gagccacatc accggaagcg cgaatagata cggaatggcg
      721 gcgatcaagc tgaccttcga tacgccgaat gatgaaacct tctccacaat tttcggcatc
      781 cagagactta caccgtaggt tgcggtgagt cccaaaaaca aagtaatgct aagcagcaag
      841 acgcgcgggt tgaccagggc acgccaggcc gggatgtgcg acgcgcgagc ttcgcgttcg
      901 cgttcaagtt cgcttgtgag ccactcgcgt tcgtcttttt gcaaccactg cgcctcttcc
      961 ggccgctccg taaggtagga caacatcatg aatcctagaa tgatcgcggg aattccttcg
     1021 aggagcagga gccagcgcca gccgttgtaa ccgagccaat gtatttttag aaaaatggcg
     1081 gagagcggcc cgccaattac ctgcgcggcg ggattagacg ccataaaaat ggcgatggcc
     1141 ttgccgcggt cttcaggtct gtaccagtgg ctgatgtaaa caagaatcgc gggcagaaat
     1201 cctgcctccg cgagtcccag aagaaaacgc agccagtaga attccttggt ggagtgaatc
     1261 aagcctgtag cggaagccag aaatccccac gtcagcaata tgcgcgcaat ccatttgcgc
     1321 gcgctccaca cttcggccag cacgcctcca ggaatttcca gcagcatgta gccgagatag
     1381 aaaattcccc cgcccaggcc gaacacggca tttgaaaatc ccagctcgcg cgtcatctga
     1441 agtcccgcgg aaccaatgtt tacgcggtcg agatacgcca cgacatagag aatcaccaga
     1501 tagggcagca agcgccggtt gacgtgacgc acggtgcgct ctgcaatgtt cgtgcgcgat
     1561 gggataacag tgtccaagac ccgagcagtc tagcacgctg aagtagcaac ttttaccgcg
     1621 agactgtctt gataaatgcg agcacaaata ttgtgggtta gagcattccg tctttgtaca
     1681 aacctgctgt ttccagaaat cgtgtaattg cctccgccgc tgcagcgccc gtctcttttc
     1741 gctacgatac tctcgcatag acgagttcgc aggcccctcg cgataggccg acccgaagac
     1801 aaaaaagatg ccccggcaaa ggcggggcat cttcgtcaca tttctaaatc tccgaccctt
     1861 ttccctagac caggaccggt ttcggaacgg tattgatcgt cttaagaatt tgggaaacga
     1921 tctggtaagg atcgccttcg gagttcgggc ggcgatcttc caggtagccc ttgtagccat
     1981 tcttaatgaa gttaatcggc atgcggatgg aggcgccgcg atccgagaga ccaaagctga
     2041 acttgtcaat cgcctgtgtt tcgtgcaggc ccgtcaagcg cagatggttg tccggtccat
     2101 agaccgcgat atgcgcatcg atgtgcttct cgaaggcctt catcagggat tcgaagtatt
     2161 ccttgccacc agtttcgcgc atatatttcg tcgagaagtt ggtgtgcata ccggatccat
     2221 tccaatcgcc cttgatcggc ttgcagtaca ggacgacatc cacttcatat ttctcgcaga
     2281 gccgcatcat caagtagcgt gcgatccaca cgtcatccgc agccttggcg gagcctttgg
     2341 cgaagatctg gaattcccac tggcccttgg cgacttctgc attgatgccc tcatgattga
     2401 tgccggcagc caaacagagc tccaagtgct cttccacgat ctggcgtgcg acgctcccca
     2461 tgtatttgta gccgacgccg cagtagtacg ggccctgcgg accggggaaa ccctctgccg
     2521 gaaagccgag aggacgccca tccttgaata ggaagtattc ctgttcgaat ccaatccaca
     2581 aatcaggatc gttaataatc gtggcgcgcg cgttcgtcgg atgcggggtg ccatcggcgt
     2641 tcatgacctc gctgaggacg atataggcat ccttgcggct tgcgtcagga tacatagcca
     2701 ccggctttag gacacagtca gagctcttgc cctccgcctg catggtggaa cttccgtcaa
     2761 aaccccagag aggcaggtcg gcaagggctg gtggactatc aaacgacttc atcgtagtct
     2821 tgccgcgcag ttcaggtaca ggcttcctgc catcaagcca gatatattcg agtttatatt
     2881 ttttcatggt gattagtgga tctccttaat gaaatctaaa gataggctgt caatgacagc
     2941 cctgagccgg taaatccgcc tatcagcgaa cccgcagaat atctctcccc aagccctctc
     3001 gtcaaatcca agaaatcttt cgaatggttc aatatttata atatcttaat gtctccatgt
     3061 aaggcaggcg cgccgggatt ccgccatggc cgtggcaaaa taccccccct gtttctatgt
     3121 gctgccaatt ggtgtaagct ttttggctca actaccgagg ccatgatgaa ccgtgtaaca
     3181 gccattttac ccgcggcggg attaggcacg cgaatgggcg ccgaaacgcc caaacagttc
     3241 ctatcgctcg acggcgtgcc cgtgcttctg ttcacgctgc ggcgccttgc ggcctgcccg
     3301 gccataacag aattcattat cgcgacgcgc gccgaagaag ttgaatcgct ggcagcgagt
     3361 gtatccaagg aaaagtttgc aaggcctgtt cgcgtggttc gtggcggcga ttcgaggcag
     3421 gattctgttg ccaatgcttt gacgcacgtg gctctcgaga cggacctgat tctagtgcac
     3481 gacgctgttc ggccccttgt gactgtcagc cagatcgagc gcgtcatcgc cgaggcggcc
     3541 gcctgcggtg cagccattct tggaattcct gccatggata ctgttaaaga agtaaaacgc
     3601 gcgagcctgc caaatgatgt ggcgctgatc acggccacga taccgcgcga gcgcgtggtt
     3661 ttggcgcaga ctcctcaagt gttccgcgcc actttgctga ttaaagcttt cggccaggcg
     3721 cgccaagatg gagttacggc ttcggatgaa gcagggctgg tggaacgcgc cggacacgac
     3781 gtccacgtgg ttgtcggttc ggagcgcaac ttgaaaatta ctcgtccggg tgacatggaa
     3841 ctcgctgaat tttatctgcg acaagagcgt gcgggacgat gagccgctgc gggattggtt
     3901 acgatctaca tcgcctggcc gaaggacgaa agttgattct cggcgggatc gaactggcat
     3961 ttcccaaagg gcctgtcggc cattcggatg gagacgcgct cgcgcatgcg atgtgcgatg
     4021 cccttttggg cgcagcagga atgggtgata ttggcacgca ttttccggac accgatccca
     4081 aatggaaaga cgcgcaaagc ctgcggtttc tggagcacat tcgcgatttg ctggccgagc
     4141 ataaatttcg catcgtgcat atcgatgcga cggtcataac agaaaagccg aaactcgggc
     4201 cgcactttcc agccatgcgt gaagcgctgg cgaaatcact gggcatctct ccctccatga
     4261 tcaatttgaa ggcaaagacc aacgaatccg tcgacgccat tggccgcggc gaagcgattg
     4321 ccgcacaggc aatcgcgacg ctcgatcgct gagttcacgg ggcgctaacc cgctttgacg
     4381 atttcaaaat tgttgatcgg gttcgtacga atgaaatcga tattgcgcct atcattttat
     4441 tttttctgtt cgacactttg aggagcactt ttcgttatgg atcgtcttac aggaatccac
     4501 gcggttcgtg aagcgctgga agcaaaccgg ccgcttgatt ccctgctggt cgctaaaggg
     4561 acgcacggaa atcgcgtgga agaattggtg cgactcgcgc gtaagctgga tgttcctgtg
     4621 cgctttgagg agcgcgctca ggtggaccgc tccgctggaa cgcgcgagca tcagggcgtg
     4681 gttgcattcg tggcctccac tcccgcgatg aatttttacg atcttttgaa accgcggccg
     4741 gaaggaagcg ctccggggtt attagttttg ctggacggtg tagaagatcc gcaaaatctt
     4801 ggcgctattg tgcgcacatc gcttgccgct ggcgcgcaag gcgtggttat tcctgaacgg
     4861 cgggcggcag ggcttacgga agccgtagct cgcgcatcgg ccggagccct ggcgcatttg
     4921 cccgtggctc gcgtcacgaa tctttcacgc gcaatggaag aaatgaagga atctggttac
     4981 tggttagttg ggctggatga gcgcgccgaa aagctccata ccgacgtgga cttcaaagtc
     5041 ccagtgggag ttgttatggg aggcgaagga aaaggattac atgccctggt gcgggaacgc
     5101 tgcgatttcc tggtctcgat tccgaccacc ggccccgtgc gcgcgctgaa cgtatccgtc
     5161 gccgcaggag tagtcctgtt cgaagttgtc cgccagcgcg gcaaaaccgg gaagcaacca
     5221 cggatgcaca cggattaaca cggataaaag ctaaatcaca taaaagcagg cacggaaatc
     5281 aggcaaaaaa atatcccatc gttgtcgtct gcttgcaatt tcaaaaactc cgtatttttc
     5341 agcctcgatc tttatccgtg tttatcagtg tgaatccgtg gttccaagcc ctggggctta
     5401 cttgtcgtac tggatcattg tgaagacgtc gatgcccggc aactttgcgc ggccatttag
     5461 aaaagttaac tcgacggcaa aagatgcgcc gaccactttt ccgcccagct tacgcacgag
     5521 ttccacggtg gccgcagccg tgccgcctgt ggcgagcaga tcgtcgctca gcagcacact
     5581 ttgaccgggc ttgatcgcgt cttcatggat ttcaagcgtg tccgtgccat actcgagctg
     5641 ataggtaaca gaaacagctt tcgctggcaa cttttttggt tttcggacag gaacaaatcc
     5701 agcgttgagt ctgtacgcca gcgcaggagc caaaataaaa ccgcgcgctt cgatgccgac
     5761 gactgcatct actttttggc ctgcgtaatg ttcgcaaaga ttgtccacca ggctatgaaa
     5821 tgctcccgga tttttcagca atgtcgtcag gtcgtaaaaa aggattcccg gcttcggata
     5881 atcgggaact tctcgaataa attctttcaa atgattcatt ccgctcgttc cgcctttcgc
     5941 aatgactcac tattcccgaa acttctgctt ctaactgcca acttcgcaat aaatttcatt
     6001 catcgttttc gattgagaaa ttgtttgcat attcacggag aatgtccgca tcgagctctt
     6061 ccacattttc caccgaggcc atgcgcggac cgcgacgcag cgcatttcgg agcgcatcca
     6121 actgctctgc gctgccgatc gcataaactt ccacgcgtcc gtcaaataag ttttttacgt
     6181 atccgttcac acccaccgtc gcagctgtcc gctcgacaaa aaaacggaag ccgactccct
     6241 gcaccattcc ggatacgaaa aatcgtttgg ccagcgtttc cacggcaatg ctctactcag
     6301 agattcgttt ttgcagacgg gcgattgtag caaaggtccc atgctcgcgg aatggaaact
     6361 gcgcgctatt tgaatccggg ttgttgcttt tggcaggccc cctcggttgc gtgggcttca
     6421 gcctgtcgat tttgaatttc ctattaacaa tggaaaaccg taggctgaag cctgtgttac
     6481 tggaagctgt cgcgatacac tgttgcagga ctgttgagcc atgaatttct tgatagccgt
     6541 accgcaaaat tgcgaaaaat tgcatccaac aaatgtcggc taaatccatg aatcaaggtt
     6601 ttaaaaattg gcgaatgctc tttgctctgg cgatgctacc gctcgcggca cccgccgcac
     6661 tgcacgcgca ggctactgga cccatgcagc caccggcctc gaccaaattt ccaacgcctc
     6721 ctccgccctc caagccagac acgcccgccc tggctcccga ggaaatcatt cgccgcttcg
     6781 ccgcgaaaga ggacgaaatg attcgcgcga tccgtggcta tacgtttcag aaaagtgtcc
     6841 gcgtcgagga aattggtccg gggaataagc ctgctgggca gttggaaatc gtcacagagc
     6901 aacgaatttc tccggaaggt aaattgtttg agaaacccgt cagccgccag caatcaactt
     6961 tgcaatatct ggatttgcag cgcggagact cggaccttgt ggcgcaagcg cccatgtttc
     7021 cgctgagtac tgcaatgctg ccgaaatacg agattgttta tggcggcaag cagccgcttg
     7081 acgatctgag cgtctattac ttcacgatta aaccgcgcgc tttggaacgc gcgcatgcct
     7141 atttttccgg agtcgtttgg gtggatgaac aggatttcgt aatcgtaaaa accatgggca
     7201 agtgggtgac ggaaaccggg gatgtaactt cctccaatct tccgttcacc gtatttgaaa
     7261 cgtaccgcca acaagttggc aagaatctat ggttccccgc gtattcccgt tcggatgaaa
     7321 cttttcagtc caacaacgtg aacgttccca tccgcatgat tatcaagtgg accaatttcg
     7381 ctccacagac aagcaccccg gctgccgatt cgccagcaat gggcaatcct ccaagcaatc
     7441 aggcacatcc atagtcaaaa tcgctagcgc caatcatcca ttacgaaaac gtcgtgccgg
     7501 ttgtaacccg catcgccatt ccgttccatc gcaaccccct gcgccgtcgc tcgaaatcct
     7561 gcctgaagca tttcacggta cgcgtcggcg cgcgcaagat tcacgccggc gaccaagctc
     7621 ttggtcccgc gtgactgggc aaattcgcgg cacgcatcaa gcaatctgcg aaacaggagc
     7681 gcgccgtcct ggccgctctg tacggcgccg aacttgatat agcattttcc gctgccagcc
     7741 tcagtcttcg cgcctacatg gcacacggca aagccggcca gcttgccgct gtcccaagtc
     7801 agcacggtgt cacccaatgt ttgactgtcc accgcgcgaa tctcccggtc tacttcgagt
     7861 ccttcataaa tcgaattcgt aagttcccgg cacgcctgca ggcactcttc cttctcgctc
     7921 tcagaaagat cgctgaaacg cagccatttt cggcggccag cagacggatt ttcgtctatc
     7981 gggagactca tcagagaagt gaggaaccgc ggccagaaac caaatttctg atacagattg
     8041 acgtgtttcg tgctggacgc aaacgtaaac aggcccgcgt gcgtgacgcc ccatgcatcg
     8101 aacaaattca tcgttggcgc caataatttt tgcgcaatgc cgcggtccca aagatcgggc
     8161 cgaattgtga gcgggccaaa aaaaccaaag cttccccagc gcgtggcgaa atttgtgcca
     8221 accagttctc cggataaatc cgcggcgaat gcagcggaag gatccacggg ccagcgggtc
     8281 cgcacgtatt cggcatcgcc cataaatttc agtgggtcag gcaatcccag gaaagtccca
     8341 aatgcgagcc gcataatccg gtccgcagcg ggaagatctt cttcgcgcaa caaacgaata
     8401 ttgacctcag gcaatttttc gagtgatgtc tgcgatgcag aattcattca cgtcctcctc
     8461 cgttcaactc ccgccgctgc ggcgaagcgt gggcctttca ggttcttcag gcgttttatc
     8521 tttaggtttc tcgccgggag gaggagcggg agctttcgga ggttgaatga tccacttgaa
     8581 accctcatcg taatcgacca ttgccatcgt aagttttgtt tctggtgaag taacggctgg
     8641 aaaagcggcc ttcataaaat ttatgaggct ggcagcgtca gctttctggc tctggccttc
     8701 cgtggccagc gtcacggact tcgcctgaat ttcattcgcg gtgcgaattt ttgtgaggcg
     8761 tccatcattc tgctgcagca gatcgagaaa ttctgcttca cccgaagctc gattctccgg
     8821 cggatagcga acttccataa acgtttttgg cttgcccttt tcgggaggat agagctggtt
     8881 ataggtaggc ctcagaacgc gcgtgcgcca aatatcgttc cccaataaat cctgccgaat
     8941 gtggtaatcg attgtccaca cctcggggcc ataatcgcgc acatatccag ataggacaat
     9001 ccgaatcaag ggctcgtcat cccccagatt cactttgtgc cgcagcagtt ctgctacctg
     9061 tcgaatccgc tcaagcaacg caattccgat ggtctcaatg tcattccctg cgtccgcctc
     9121 ttttggcttc ccggaagaat taaaagcggc cgcgataatt ctgcgaaatt cattgtcgag
     9181 ccggatggta tcttccttgg aatccgggcg ctcccactcc acggccccca gcatcacgcc
     9241 catgcgatcc gcggagagta cttcaatcgc cggcggcttt gaagtagcct caccatgcgc
     9301 attcatcgcc gccaaaataa ttccatcttt agccgcgcag attacgacgc gtccttccgc
     9361 aagattcact gcaacttcct gatcttgcgc ctcctgcgca cgcgctaccg gaggtaggaa
     9421 ccacagatgc acgcagatga acacggataa gagaaagaac gaataaggga aagaaaaagc
     9481 attcttgcga agttttaatt ccatggttgc aatccagaat cgaacatttc caaattctcg
     9541 ctctttttct aaatcatcat tgattcgctt ttatcagcca ctttttttat ccgtgctgat
     9601 ctgcgtgcat ctgcggttcc aacgtttttt ctttccgtgc gatagcctgc agcgacgtaa
     9661 gggcgacgac attgacgata tcttctgacg agcatccgcg agagagatcg ttcgcgggcc
     9721 tgtccagtcc ctgcagaatg gggccgatcg cttgcgcgcc gcccaagcgt tccacaagtt
     9781 tataggcaat gttacctgaa tcgaggtccg gaaaaatcaa cacattggca tggccagcca
     9841 cgggtgaacc cggcgctttt gaatttgcca cactagccaa aagcgccgta tcagcttgca
     9901 attctccatc cgccgcaatc tgtggggctc gcgccttcag aatacgcagc gcctccttaa
     9961 ttttttcaac ccgcggatgg cttgccgagc ctttggtcga aaaggaaagc agcgcaacgc
    10021 gcggttcggc atcgagtatc gcgcgcgcgt tttccgctgc agcacgcgca atttcagcaa
    10081 gctcctcggc attcggatct ggtaccactg cgcaatccgc aaaaagaaaa gccccgccag
    10141 tggacatatc caggcctgct cgctcgggaa ctagcatgac gaaaaagctg gaaaccagtt
    10201 tcgcatcgga agccaagcca acggtatgca atgccgcgcg caccgcttca cctgtcgtgt
    10261 ttgctgcgcc tcccaccgat ccgtccgcgt cgccggcagc gacgctcaac gcggcgaagt
    10321 aaagcgggcg tcgagcaatt tcgctcgctt cttggagcga tgcacccttc gcacgccttc
    10381 gttcataata aatctgagca tagacttcgc cacgttcact cgtcgcagag tctacgatcg
    10441 tgacctttgc caaatccgca tacatatcag aagctatagc ggcaataatt tgtttccgtc
    10501 caagcaacgt aattttcgcg aagccttccc ggctgaccgt tgccgcagca gcaacgacgc
    10561 gagggtcttc gccttccgcc aacacaattc gctgaaggtg tgagcggacg cgattcttaa
    10621 gcttctccat cagagtcatc acctgcccca aggtaacaca ggtttaagca agatcggcag
    10681 cgagtcgttc ccaatcgttt ttcagaagca attcctcaat ccggtacgca tgaaacatgt
    10741 catgcatcgc caagtggcgg aacataatga aaactgaata gtgcgtgtac tcgccgtgct
    10801 cggctctacg cccccaatcc tcgggtgaaa gcgtcttcaa ctgatcgacg agtctttggc
    10861 gatcccgagt gaatctctgc agcgcttcgt ccagatttgt tttgagcaag gcatcttcct
    10921 catcgtcaac gtgaggaagg tacggcctta tgcgtggcgc tggctctgcg agcatcaaat
    10981 ccaggcgcga tgacatcagc ggatgtacgg cggcgaggtg gcaggcatgt tcatgagcgg
    11041 accattttcc cggcttcggc cgccgcttta ggatttcttc cgggacttca cggacaagcg
    11101 ggagaattat ttccggagag cgctccagag cggagataat ggcattaaag ttttccattc
    11161 gacctcacta gcgaatgttc gctttcaatt tgctcatccc acggattcca gtagaatgct
    11221 agagcaaacc ataacaatcg cgcgacggca agctattccc acgatcgtgg gacgatgcaa
    11281 caatgccaag gaaggcggac accattcgac acaaaggcaa gaagctagag gacatcctgg
    11341 tagcgcacga aaaatttcat aaggggcatc cgggcggcga acgcgccgac ctgtctggtg
    11401 cgaatctgtc cggccaggat ttaaagaagc gcgatctgac cggcgcaaat ctggtgggaa
    11461 gtaatcttca gaaagccgat ctgcgcggct gcaaactcag ctttgccgat ttgagcaaag
    11521 cgaatctgcg aaaagccgat ctgcgcaatt gcgatttcac ggaaacgaaa cttgagggcg
    11581 cggatttaag cgaagcgaat ttgagcggaa cggaattgtt ccgcggcgat ttacgctccg
    11641 cgaaattgca caaaactatt ttacgcggca cagtcctgag gcaggcaaac ttgcgcggcg
    11701 cggatttttc aggatcggat ctcgcgctgg ccagcttccg cgaaatcgat ctaaccgatg
    11761 taaagatgga tggcgcagac ctgaaggatg ccgtggtccg cgataatcgt aagtcgtaaa
    11821 tgtagcgccc gcctaaaggc gggcgctaca gaattatctt cctgtggccg atgcagaaga
    11881 ctctgaggcg ttgcgcgtaa ttccccggcg gcgcacgtca ataccaaggc gcttaatttt
    11941 ctgattcaaa gtggaaagcg gaatctgaaa actttcggcc gcttccgttt gattccaatc
    12001 cgtgcgttcg agcatgccga cgattacgcg ccgctcaatc tcatccataa tttgaaataa
    12061 ggaatttttc gcactatcag cgcccgagcg cgaaccgtgt tcgccgggta atggcggcgc
    12121 aaactcggaa agatgcagtc tgactccgcc cagcagttcc ttcgtacgga cgctctcggg
    12181 caagagttcc acatccagtt gttcttgaga ggaaagtacg acggcacgct cgacgacgtt
    12241 ttcgagttcg cggatatttc ccggccagtc gtaatccatc agcaaacgca acgcaggttg
    12301 agtaaagtac ccaaccgggc gcgcattctc tttcgcaaac acattcagaa aatgatcgag
    12361 caatagtgga acatcttcct tccgctcgcg aagaggcggc agaggaacag caatcacgtt
    12421 caggcggtga tataaatctt cgcgaaatcg tccctcgcgc accagattca gtaattgcac
    12481 attgcaggca gcgataatgc gcacatcgac cttgatcgtt tccgtaccgc ccaggcgcat
    12541 gaattcgcgc tcttgaatga cgcgaaggag cttggcctgc gtctccggcc cgatcgtgga
    12601 gatttcgtca aaaaatatcg ttccctgatc ggcaacttcg aagagtccct ttttcgaggt
    12661 cacggcgctg gtaaacgcgc ctttgacgtg cccgaaaagc tgcgactcga gaaggtccac
    12721 tggaatcgag cccgtattga tcggtacaaa agccttgtcc gcgcgcgtgg atgccgaatg
    12781 aatcgctttg gcgatcaact ccttgcctgt tccactttcg ccatgaatta aaaccgtgga
    12841 gcgagaggga gcaacctgac taacaaggtc caagacggcc agcatcttct cgcttttccc
    12901 gatgatgttt ggaaaattat agcgctgctt cagagcgcgc ttgagctgta cgttttcttc
    12961 gcgcagccgc tgcccttcca cagcctgcgc cacctgcacg agcaactcat cgtttgacca
    13021 cggcttggta atgtaatcct gcgcgccgct tttaaacgct gcgcgcgcca tatctattga
    13081 gccgtacgcg gtgatcagaa ccaccgcaag ccccggactg cgccgctgaa tttcgcgcag
    13141 cacatcaagg ccgtttcgat ccggcaggct tacatccagg agcacaagat cgaaaggttt
    13201 ttcatcgagc atggcaaggc ccgtctcggc gctatcggcg ctcgaggtcg agtacccttc
    13261 cacgttgagg agaagttcga ggccttcgcg aatctcggat tcgtcgtcaa ccaccaggat
    13321 ggagccctta gacatgaacc gctttccgcg caacggggaa ttccaatcgg aacgaagtgc
    13381 ccttgcctgg tgtggagcgc acatccactt ttcccgcgtg ctccttgaca atgccatagc
    13441 tcacggacag gcccaaccct gtgccgcgtc ccgatgattt cgtagtaaaa aatggatcga
    13501 atatccggtt cagattttcg cggggaattc cgcctccact atcgatgatt tccacttcca
    13561 ccgaaccgtt atgcgccgca gtgcgaattt ccacgacacc gccggagggc atggcatcac
    13621 gcgcgttcaa aaataaattc agaaaaactt gctgcagctt attgtttgag cctagaacag
    13681 ccggcaattc ctgctgaaga tttctcgtga cttgcacttg agccgtgcgg aatggatgcg
    13741 cgaccagcgt caatacttct tccaccacgg cattcaaatt cacttcggta aattccgccg
    13801 cgccggtgcg cgaaaaattc agcagattgt ttacgatttc gctcgcccgg aatgtctgct
    13861 tcaccacttt ttcaatgagc tgctggcgcg ggtcgctcac gggaagctgc ttggccagca
    13921 tttgaatata attggaaatt acggcaaggg gcgtattcac ttcatgcgca acaccagcgg
    13981 caagcaagcc aagcgaagtg agtttctcgt tctgaaatac ctgctcctcc aggcgcatgc
    14041 gctgcgtaac atcgtcaaaa agtataagcc gcccgatgcg attcccggac ttcccgacca
    14101 gcggagcaat cgaaacattg accacgagat tccgcccgtc gcggcttcgc attggaaatt
    14161 tatagaggct ggaaacacgt tcatcggcgg aacgcgccgc aatttccgcc atcaaatcgg
    14221 cgggcagcac atcttccagc ttgcggcgga ccgcttcctg ccggggtacg ccaatcaatc
    14281 cttcgagctg cgtgttccag gattccaccg cgccgttgaa atcgaccgcc agcacgccca
    14341 cattcagcga ttcaacgata ttttccgaga aatctttgag ccgttcgatt tgcgccgctt
    14401 tttgttccag agagttgtag agctgagcat tgtcgagcgc aatggccaga taaccggcaa
    14461 tcgtgtgcag tagttcgacg tcttcactcg acagaaagtc tccatccacg gttttgccca
    14521 agcccagcac ggctattgcg tgatcgcgaa tctggcatgg aatgaaataa tttaaatcga
    14581 gttgctcgag cgtgtaacga accggcggag aagattcccg cgcggcacgc gctgattcat
    14641 aaaaaaggac gccctgcgcg aacggtttgc attcctcatc cagaaaactc atgtccagcg
    14701 cacctgaata ccgcacgccc atggatcgcg cgatgcgata ggttccggga cggctttcgt
    14761 cctcaataaa aatggccagg cgatccacca gcaacgcctg tgagatccgg tccatcacag
    14821 aggcgagcat gggatcgagg cgcacttccg acgtgagcgt gcggccgaat tcaatgagcg
    14881 tgcgacggta atccaggcga tctcgataga aaaaacggtc gaggcgcgct tggatatagt
    14941 cacgaaatgg ctggaacaaa aacgccgcca ctacaatcgc gatcactccg cccatcgttc
    15001 ccgaaggcca ggcggtgtgg aatagcgctc caatcagcgc cacgattgca atgtagactg
    15061 ccacaacgcc gccgcttgcc gcagtatacg caagaccgcg cttgaagata atgtccacgt
    15121 ccatcaagcg gtagcgaatg atggcgtatc caaagctcag tgggataagc accagagaaa
    15181 ttgtcgagaa gttcatccac gggcgcggaa ccacacccag gaaaaaaggc acaatgtaaa
    15241 gtagaagaaa cggaacaatg cccgcaagcg ctccaccggt aacccatttt aattgctgac
    15301 gcaggattcc ggacgaagca cggcggtagc tggtaacaaa aactagcgca gcaagcgaaa
    15361 agtaaattcc aagacacgcc agatcgaggc gatcgagagt gtttcgcgcc tccaccgacg
    15421 gcataaaacc caattcgcga aggcccacaa gaattcgcac gatcagcaga attccgggga
    15481 ctccataaat tgcagcaagg atcgtcccac gtccttgaga ggcgggccgg cgctccggaa
    15541 ataccattgc gaaatgcagc agcaacgcgg gctgcagcaa tctggctacg atggcagccc
    15601 aataaatttc ccagtcaaac gtggtcagct tgcccgtgta ttgaaatgag taaaggacaa
    15661 aggagaccag acaaaaaatg tagaagtgca cggcgcgtgt ggcgttccaa cggcgggcaa
    15721 aaatgaaaag gccaataaaa aggtacagca cgcctacaac actcaaataa ttttcaatcg
    15781 acgagggtcg ggcagtcggc tcgattacga gttgtacctc aaatttcttg ccgccccgtt
    15841 caatctggta atgggccagc gaccacaacc caattcgcca caagcgcttg gtgacatcaa
    15901 tcggaccgcg gatcggtaca tcgttgatgg caagaacttg atcgcctgtt cgaagacccg
    15961 caacctcacc tggagacttt ggtgtgacat gccacgcgac cacgccggat gggtttgcga
    16021 tccaagaaac cccgtcatcg ggtgccgtaa aaagtgtacg ttgttgaaaa ttgatgatgc
    16081 cgaagacgat cgccgccaga gttgccagcg caaggactgc cgctcccaac cgaactcgaa
    16141 gactatcgtt aaatggggcg ctcaacggtt attcccacaa acagcggacg gaacaaatag
    16201 cgtgcaaacc tgcttccaaa ttatataggt tttttcaaat tttcaactat atgaaaataa
    16261 atagcatgcg tgcaaatttg gcataaaaag acacacggca tagccttagt ctacggttaa
    16321 tggaatgcat cttcctgttt ctaatagcct tctggggcta aaactggaag ctgacgccac
    16381 cccggaatga acgtaatatg ggggctaata tcatccgagg atcctgccca ctgacactta
    16441 catagccctg cgccaaaagg ttgctgaagt cagcaagcgc ttcccagcgg ccgttcaagc
    16501 caggcaactg ctggcggatg gagatgtgca ggttcggatc catttggtat gcagcttcgc
    16561 caaattggtc cattcgagat agcgcgctgc cggcaaccca tttgtagctt gcggaaaact
    16621 gcgtcccgga ctgcgaaatc ttgccggaaa ttcgcgccgc aacgctgtga tgattgctag
    16681 tcgtaaaatt attgcggata gcggaaggcg aaaggtccgc agccgatcca ggagaaagag
    16741 cgcctgccca ggaataaatc gtggcgactt cccaggcgtc ggatatcttt tgcctgtagg
    16801 ccacacgagt accccaggaa ctggaattgc caccgtcata gaggaaagcg ctggaaaatt
    16861 cgtcctgaac aaattcaggg ctcaccgatg ccccagttcc aaaaatcgcc tgatcccggg
    16921 acgagtcgtg aaacgcagcc acttcaattg cagattgttt accgacccgc ctcttcgcag
    16981 acaattcctg atgccagccg gcttccagaa caggccgccc actatggaag agaactggcg
    17041 ggagactatt gagctcatcg atagccgatt ccagcgtgcc ggagcgcccc cactgctcgg
    17101 agggaggatt tgaagcgaca atcaacgaaa gcgtcaagtc cggtgaaact gtggtattta
    17161 attgcaagtg gggacgtaaa gaagaagtcg cggaaataat cccagcacga agatactccg
    17221 cgccaacttt taactgcaca tgatcgccca gttcgatctg ttccgtgtga tctaggcgca
    17281 ttccttgaaa ttccatgccc tcagcgccga ttttcgactg ccgccaaatg aaaatggtct
    17341 ctggaccatt tccagcgccc gaaggaagcc agacgccagc gaaggaactg gatgctccct
    17401 tgtcataact catttgacct gctaccagca ttcgaccgag cttcccgatc ttctgatcat
    17461 aggaaaccgc agtcgcggga gcgttcggga agttggaagc cgagcccggc cgcatggctc
    17521 cattccctac ctggaccaat gctcgcggac gctgcgcgct gggaagatcg ccgccgattg
    17581 cggcggaggc agtttgcgtt gtgctggtgt cgtcccactg caaaatggtt ctcgtagccg
    17641 ccgaggaccg caacacccat ttccagtcat cctgctccga agtggcatcg ggcttacgac
    17701 gcagtgtatc aagcgaagaa aatactgtgt ccacttgcac gcgcaggagg gtcgtcaagt
    17761 tggacatgac cgcgacatga tgctccatcg cgggaagaaa tccggccatg gatacacgta
    17821 catcataagc gcctggcttg aggtgatcgg taataaaggt gccacgctga ttggaaagaa
    17881 tttgcgcaac gatgcggccg ccgtcttcgg agatcagcca aacactcgcc cccatctgtg
    17941 gcgttccaga ggagtctaaa accacacccg agatcgttcc ataccccggc tttgccgcag
    18001 tagaagcagc cattcccaac gtaactacga tgacccagcc cagagttcga aaaatcgact
    18061 gcatgggaac tggttacctt cctgtggctc cgtttgttgc caccttcgct aacatcgcgg
    18121 gaggagttac agagaaatcc gcggaacgcg taaccgtttg attggccacc aaatctgtcg
    18181 cttgaatttc cactttgtat ttgccgggag ctaatgtttt cggagcaata atttgctgca
    18241 ccgtgatctg ttccccactc tgctggattt gatctccggt cttaacttca tgggaaacca
    18301 cctggccatt ctgcgtgatt ttgaaatcca atgacacatt atttctgtgc gtcttgtcat
    18361 ccactttcaa attgtaaaac tgcaggtaga tatccagcgc ctggtcggcg tgaaattccg
    18421 catccaactt ggggcgcact ttggttgagc ccaggacgaa ttggccgacg cccacgtcct
    18481 tggatgagac gggctccatc tggtccgcaa ggatcagcga gctggcttcc agcttgtcat
    18541 ccggaatggg agaaaccgcc aggcgcgtat ttacaacgcc aacgttcccg ctgtttacat
    18601 ccttgatcac cagatccagc ttgtacaaac cagggcgcag cggtatagcc ttctgataaa
    18661 tcgaaaaacc ttttagagac ggctcaagca aggaatcggg aaaatcgcgc tggataacat
    18721 cttcgaaagt ttgaatcacg cggcccgtca tggaactgac gcgtccaaac aaattcagcg
    18781 aagcggactg cactccgtcg tgatcccgga aagtcatttg tttgttttgg atttgaacgg
    18841 ttaccggcac gagaatggtg tcgccggtaa tgcgcatgaa atcaaaacga taatcgaagg
    18901 aaatctggtt gcgaagaatg cgtgagctga ccacttcttc gaggtcttta aacttgaccg
    18961 gaggaggtgc ttgcactttt gcaaattgtt ccaagcgcgt gaattcgttt tggctttcaa
    19021 gcccgccacc gccattcact tgagctgagc ccaatggcgc ggtcgagccg ttggactgtg
    19081 tgaaacgctg agcttgggtg gacattccca tggattccag caaactgggc ccaccgcccg
    19141 gaacatgggc catagcatct ttttcgcccg ggtccatcgt aatgtgatac tcgccggtcc
    19201 ccgttgggtc cacaaactcg atggtgatgt tttgcccgat tccttccata tagtgataat
    19261 tccaatcgtc gaatccataa actgtggtct gaccgccgcc ttctgtcatg gggcggtcat
    19321 aagtactgcc ggctgaatgg gattcgatgc tatcgggagg tccccacatg atgtaaattc
    19381 ggccgcgatc agtccgccat ccgggaatgc ctgaagaaaa tcgctcattg gcgtaggcga
    19441 tccggcggta atgctcttcc ttaaattcgt tctccacggt atccggcgtt gggtcgcgcc
    19501 tctgccagaa cccttcaatg aattgctcgc gctcttcgtt ggtttgcaat tgcaggaaag
    19561 ccgagcgctc ttcatccgta ataatgtacg ggacttcttc ttccaaccat ttcttgtagg
    19621 gagtggcaag ttccttttgg agtttcttta ggttcttctt gccgtcgttc tttttctttc
    19681 ctgaatcttg ttgtttgtcc gcggaagcgg cgctctgatc tttatcctga tcctgcgcat
    19741 ggagacgcgg gctggctggc aaaaggaatg ctccgccaat caacaagata actgacatgc
    19801 gttccaagcg attcatacgt acctcacccc tgatctcgga gattggattc tatccccggc
    19861 ctttgcaacc gtcaatcaag gaatctccgc aaatcgacct cacccacatg tgctctctca
    19921 gctgccagcg aataagcgga atgagcaccg atggatacta agaactacga gggagctgtc
    19981 gggttgcccc aagatttcca cagttttcgc ttgtttggct tgggaggagg gattcagggc
    20041 gccgatcaaa ccggggatga gcacgcttcc gccttacccc atgcattggc gcgttctagt
    20101 gtcggaatga gttgccggac agggcaaact cctttcctac gcatcaaatt acctgtaaaa
    20161 ttgaaggttg aatgatgttc gttttcccaa ccaggagaat ccaggcttat ctcaccaatt
    20221 caatacggaa tccactcttg caggcaacgg ccaacaagag ttgaaagttt tcactccaaa
    20281 taacgtacac aggagaagga actcgaaccg cgcaattaaa accgcacacc catgaacatg
    20341 tggaaaaaaa tcgggatagg agtgggtgtt gccgccgttt tggccggcgg agtctggatt
    20401 gcgctctggc agatcaacaa gggcgtcgct acggtgcaaa cagccccggc gctgcgccag
    20461 gatttaaatt cgattgtaac ggcttcggga gaagtcaagc cgttgaccta caccaacgtg
    20521 cttggcgaag gaatcggaaa aattacagaa attgcggtga aggaaggcga tcacgtcaag
    20581 aaaggcgacg tgctgttacg tttggaaagc gtgcagcctg ctgcggacgt caacgcgcaa
    20641 cgcgccaata ttgcggcggc tgaagcgggt ataaaatccg cggcggctaa cgacgtctcg
    20701 tcgcaagcgg acatcaaatc gcgtgcagcg gacctggaac acgcaaagct ggattgggat
    20761 cgtgggcaac ttttgtttaa agacggcctg attcccaagc aggattacga tactcgtaaa
    20821 gcttcctacg atagtgccgt agcggccttg gcatccgcgc aagctcgcgc tgaacaggct
    20881 cgggcgcagc tcaaccaagc acgttcggga ttaacgcaaa atcaggccat gctcggccgg
    20941 ttgagcgaca ttttgcacaa gaccacttac acggctccca tcaatggagt cgtcacgtac
    21001 attgcggtgc gagtgggcga aaacgtggtg ccaggcatcc aaaacgccac gggaagctac
    21061 ctgatgacga tttccgatat gtccgtggtg acgtcggagg tgatggtgga cgaaaccgac
    21121 attgtcagcg tcaagttggg agaagacgcc gaagtcacca tcgacgcgat tccaggaaag
    21181 tttttcaaag ggaaagtaac ggaagtcggg acgcaggccg tgcttcgcag ttcgggtctg
    21241 gcttcgacgc aatcgacgac aggaaatcag gaagcgaagg attttaaagt ggtagtgacc
    21301 ttgcaaaatc cgcctgatgg cttgcgtccc ggtttgtcct cgaccgcgaa aatcaccacg
    21361 gcgcaaaaga aaaatgtggt gaccattccc attcaagcgc tggcggaacg cacgaaaaaa
    21421 gatctggatg aagcggccaa ggaaaatggc caaaattatg tcacgctggc ggcggaacgc
    21481 cctgcaccgg gacccgatgg atcgggatcg acttccgccc tcgaaaaaaa tccggaaatt
    21541 cagggcgtgt tcgtagttcg caacaagcgg gcgcaatttg tggccgttgc cacggggatt
    21601 accggaatca ccgatattga aattacaagc ggcttgcaag agggcgatca aatcatcacg
    21661 ggaagctata aaactttgcg caccctgaaa cccgatgctc gagtaaaagt ggataactca
    21721 acgcccaaag cgccagaaga taactaaatg aaaatttccc ccggattgag gaactgagga
    21781 atggccgagc gagccgtctc gaacgaaaca tttcgccagg accttgtcga tcagggtgtg
    21841 gccatcctga ccgaaggact ttggaaaacg tatgaaatgg gcggtgaaaa gctacatgcg
    21901 ttgcgcggcg tggatctcac cattcgccgg ggagaatacg tcgccattat gggaccgtcc
    21961 ggatcgggca aatcgacgct catgaacttg attggatgcc tcgatacgcc aacctccgga
    22021 aaatattggc tggccgggcg gctcgtaagc gaactcgacg atgatgaact cgccgctatt
    22081 cgcaacaaag aaattggatt tgtgtttcaa acatttaatt tgctaccgcg agccactgcc
    22141 ctgcataacg tggaactccc catggtttac aacggcactc cggcggaaga gcgcctcgcg
    22201 cgcgcaaagc gcgccctcga agccgtggat ttaggcgaaa gaatgtttca taagccgaat
    22261 gaactttccg gcgggcagcg ccagcgcgta gccgtggcgc gcgcgcttgt gaactcgccg
    22321 tccatcgtgc ttgccgacga gccgacagga aacctcgata ctaaaacggg cgatgaaatc
    22381 atggcattgt tcgcgcgcct gcactcggag ggcaacacga tcattctcgt gacgcatgaa
    22441 aacgacattg cgcggcgcgc caaccgcatc atccacgttc gcgacggcaa agttgaaacc
    22501 gatgaacccg tagcccacta ggccacgcaa cttatgaaga aactctttcc tgcagctctc
    22561 ccttaaggat gtattagcgt gcgctcaaga atttccgtgt tcatttccat cgtccaggcg
    22621 atcctgctac tggcgcatct atttttgtat gagacatgga tttttttcca tgggaccccg
    22681 gatccccctt atggatttgc attgaaaatt gttttatcga tattgtccgt aagcttcgtc
    22741 gcggcttccc tgcttgcgca gaaatatttt aatccgctgg ttcgagccat ttatacgatg
    22801 gccgccgtat ggcttgggct ggtgaatttc ttttttcttg ccgcgtgcgc aatctggatt
    22861 atttatgggc ttccgctgct gctcggattt cacttggaaa aacgagatct cgcaacgcaa
    22921 tgttttggcg ccggccttct cgcgggaatt ctggcgctcg cgaatgcggc atggccgcgc
    22981 gtcgtgcgta tttccatcaa gttgcctaat cttcccgctt cctggcgtgg ccgcaccgcg
    23041 gccctcgtga gcgatttaca tctcggccat gtgcgaaacg gcggcttcgt tcggcgcatc
    23101 gttcggaaac tttcgagcct gaagcccgac gtcttattta ttcccggcga catgtttgac
    23161 gggacagccg tcaacttcgg ccgattgtcc aaaacatgga aagaattttc cgcgccgctc
    23221 ggttcctatt tcattgcggg caatcatgaa cagtttgcca gctatgaaaa atatttcggc
    23281 gccgttacgg cttccggaat ccgcgtcgtt cataacgaaa aaattattct agatgggttg
    23341 caggtagtgg gcgttcacta tttggattca aaaaacatgg aaagatttcg atcgatcctt
    23401 gcgaaagccg agttggatcc gaacatagcc agcgttctgc ttgttcacga tccaagttta
    23461 ttgtccatcg ccatggaggc cggaatttcc ctgcaattat ccggtcacac gcatcgggga
    23521 caattttttc cgttcactaa aatcgtctct cgcatttacg gcatctacgc gtatggattg
    23581 catcacttcg atgctcttca agtttacact tcctgtggtg ttggcacgtg ggggcctcct
    23641 atgcgcctcg gctcaaatcc tgaaatcgtc ttgattcatt ttgaatagtg cattgctatt
    23701 caggattatt ttttagcgga ccttcgcgcc ggggaaaagg acgaaagaac agaatcttcg
    23761 ccgaatcaca gtgtttcgtt ccggaacaca aattcctcca gcgaacacct attgattaaa
    23821 gttcagatac tccctgccga tgatggttca tgaacgaccc aaacctagct gctccgtggg
    23881 cccgaaaagg atgttcccac cgcggaggta aaacgatgcc ggttcaaagc atctcgagtc
    23941 ttattcaaaa tctttcggat caattatttg gacaagcgca agacgataat gcccagaaaa
    24001 gtctactcgg gctagctagc ccggtaactg cggcaatagc ggaagataca tttaccccgt
    24061 cgacgcaaag cggtattgct cagcccgcag agcaagaggc ggggctattc caattcgcgg
    24121 ctggaactct gacaacgaac caacctggtt caacgggacg gcctgcgatc ggagcgcctg
    24181 ctatatcagg ggcagcgcaa acagctgcaa cgacgactac ggccgaagcg agcggagctt
    24241 cagggcaagc agcgacggga actccagcaa ctcaggcggc cacgagcgca gcagcggcgg
    24301 caaacatcca gcttcaaatt cagtcactca atgcggccct tccagccctc gggctgacca
    24361 acacggaaat aaaccagatc gaccgtattg catcacttgt ccagaatttc aatccggcag
    24421 cctatgcaaa tcttgtcagc cagtttgaag cgctggcgca aaattcggga tcgccgaatt
    24481 cggctgcgat tccaagcaca aacagcgcaa ctcccgcggc taactcccaa aatggtgccg
    24541 gttatcagct tcaggaaatc tctatcaaat ttacgggatc cacacagccg ggaagtagca
    24601 gctttgcagg cggcggaggg aaaaactccg atcctaatac gccccagtct aattccgcag
    24661 ggctgcaaat cggccaagtg cagttcacgt tgatcaacgg ccacgggcaa actgtgcgag
    24721 tgcaggcgct gtagttcact ttcatctcat tccattctca accgtgtttt tgtgacacgg
    24781 gattcagcct gcggaagata gtctttgcaa gggctaaatt ccagaggctg aagactgcgc
    24841 cacttaagcc gtcatgatat aaattcaatg atcatttttc gtccacatgt ttgcatcgcg
    24901 cacaaattgg catctggaaa cgaaccgctt gacgcgtgct ctcgaagagc accgaaaaag
    24961 tggcaaaaag ctttttgatt taaccgcctc caatcccacc gagtgcggcc ttgcatatcc
    25021 tgagcaggaa attcttactg cgcttgccaa tccacgcgcg ctcacctatc aacccgatag
    25081 taaaggtttg ctcgaagcgc gagaagccgt cgccgcgtat tacgctggcc gcccagggtt
    25141 ttccgaatct cccgcgcccg ttgatccgga gcgcatcatc ctcgcatcag gaacaagcga
    25201 ggcatacaca cacatttttt gcctgctgtg cgaagacggc gacgaaattc tggttcccgc
    25261 gcccagttat ctgctctttg aatttctggc ggacctcgcg gacattcgtc tcgttcccta
    25321 tccgctgctc tatgatcacg gttggcaaat cgatttcgct tcgctacgtg cggcgctcac
    25381 atcgcgttcg cgggccgtcc tgattgtgca ccccaacaat cccactgggt cattcgttaa
    25441 gcggcacgaa gcaacagaat tggctgaaat ttgtgcggcg cgggaaatgg ctattgtggc
    25501 cgatgaagtg tttctggatt atgcggagaa tacgggaaat cgattgacgg gcagtggtag
    25561 cgccaacctt ca
//
LOCUS       DASZMB010000214         4834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4884051, whole genome shotgun sequence.
ACCESSION   DASZMB010000214 DASZMB010000000
VERSION     DASZMB010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4884051"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3026)
                     /locus_tag="VG272_10075"
     CDS             complement(<1..3026)
                     /locus_tag="VG272_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14070.1"
                     /translation="MRFHRGFCAFPKFVIMCLLATPLAASPASPPQRTSSKDAKPADV
                     ASIPGPMRSFLRMAAISQQITPDELLPLLARNVVMSGYEVGRPTQFLVLLNWYLDQAR
                     ELEALAGKAQVLHVSNCDDAKPLLSVLGYRLQKSCGPDAALETADANRAFLTVNSGFP
                     LADLEDSLREGKPIDIPYGPTKVPVLYKTSDWVPAKKNSNRGVVDALLRDPNVARLYW
                     AMSRMDSETAQLLWQTMGFKKLILVAPVLDFYGSQLSVRSGRVLVPGGASSESAWRDL
                     VGANPRSPAEFISKLLIKDGGWAASYFDTLSRVGPTQQGYFTSSRRLVRMYEALRGHD
                     LNPLPTKHSFRPDPGLFLLEARLMLDPSGQPHIPGGIEVWKEVLHRKFDSKLVREWGD
                     KASSWNNPEQVVEGMVAVTRYPMPDGPVYNFLMLTEIDRNRAPGEQLNTATARLLAEK
                     FPLFGDQYPRFAEFHALNNGSITRFLKVAEGLNQIPDRLARTDALGMMQANVGLWEIL
                     ARQGEIPDAQLNDSWEKVLIPFSTLHSSEQVYEAGRASFAELLRAATGKTSISQDQLF
                     SVLAGPEQYNKEGQQLRLQMARRMHQVMDDQRLVSLDTLITLGDGLDQMAKGKGGADV
                     LLPLAAELREFELPRPLFTKSERNEFAAGLYSNRHTALQMRTDLIRIIKSANHDELMQ
                     ARALLTPFLRDTLVGLNYAYYEPPGAQMIHNNPLFVRSHDFSGGDYSGEEATASDQIW
                     RSPRIFGRGWTASGGAHLIGSMADLPYALARVEQDFIVPQNVQSLIWEDLVPGLLTSA
                     VLPRWWNVNRNEMHAVALYQRTGEELLSSAAKDPKVREQVLAIISERLLPQRLESIET
                     ALRNGQEEDALALLAPAEALYLAAEFRRTHSTENQAWGPSGNELAQVLDRYPIETNLE
                     RISEDFGVPHPALAQNYHRELMTIKPVPTFLYYSSRLMAESWDSGNLYWGRLADEMGY
                     SPVMLNVLVPELTRRMIEQTFASHLEDWSAILRAMH"
     gene            3498..4385
                     /locus_tag="VG272_10080"
     CDS             3498..4385
                     /locus_tag="VG272_10080"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWF14071.1"
                     /translation="MKIAFLGLGKMGAGMARNLMRAGHTLAVYNRSRGKAEALAAEGA
                     RIADSPADAARDADAVMSMVADDHALEAVIFGEHGAASAMKEGCVHLSHSTISTAMAR
                     RLTSEHAQRKQVFLSVPVFGRPDAAENKNLVVMVAGPAELVERCRPLFDAIGRVTFVV
                     GAEPWQANVAKVCGNFMVISAIEALGEAYATLRKAGVDPEIFLDVMHSLFGSNVITGY
                     GRIIAKEQFEPAGFALKLGLKDVRLVLAASEECASPMPLASLVHDHLLAGLAQGQGEM
                     DWSSMTQVIARNAGLKAKS"
     gene            4506..>4834
                     /locus_tag="VG272_10085"
     CDS             4506..>4834
                     /locus_tag="VG272_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14072.1"
                     /translation="MSLVDFIATPKFEEYRERFKQHYRMERSKDGVIVVEAHTVGGPI
                     QLSVENHRSVGQLFKTIGADPENEVMIFTGSGKDFMMDADPEGFKLEQEDLQHWAYEY
                     AYKDGRI"
BASE COUNT         1095 a         1243 c         1435 g         1061 t
ORIGIN      
        1 tgatgcatgg cgcgtagtat ggcggaccaa tcttccaagt gcgaagcgaa agtctgctca
       61 atcatgcgcc gggttaattc gggaacgagt acgttcagca tcactgggga atatcccatc
      121 tcgtccgcca ggcggcccca atagagattt cctgagtccc aggattccgc cattaagcgg
      181 ctggaataat aaagaaaggt ggggacgggt tttatggtca tcaattcacg gtgatagttt
      241 tgcgctaggg ccggatgagg gaccccgaaa tcttcggaga tacgttccaa gtttgtttca
      301 atcggatagc gatccagaac ttgagccagt tcgtttcctg acgggcccca ggcctggttt
      361 tctgtcgaat gggtacgccg aaattcagcg gccagatata gggcctcggc cggagcgaga
      421 agcgcgagcg catcttcctc ttgtccattg cgcaacgcgg tctcgattga ctcaagtcgt
      481 tgaggtagca ggcgctcgga aataatggcg agcacttgtt cgcgcacctt cgggtctttt
      541 gcggcggaac tgagcagttc ttcgccggtg cgctgataca aggccacggc gtgcatttca
      601 tttcgattta cattccacca tcgcggcaat actgcactcg tgagaagtcc gggaactaaa
      661 tcttcccaga ttagcgattg cacattctgg ggcacgatga agtcctgttc gacgcgcgcc
      721 agggcgtacg gcaaatccgc catggagccg atcagatgcg caccgccgct ggccgtccat
      781 ccgcggccaa aaatgcgcgg cgaacgccaa atctgatcgc tggccgtggc ctcttcgccg
      841 gaataatcac cgccggaaaa atcgtgcgag cgcacgaaga gtggattgtt gtgaatcatc
      901 tgcgcgccgg ggggttcgta gtaggcgtaa ttaagtccca ccaaggtgtc ccgaaggaaa
      961 ggagtgagca gggcacgggc ttgcatcaat tcgtcgtgat tagcagattt aataatgcga
     1021 attaaatccg tacgcatttg cagggccgtg tgacgattac tgtaaaggcc cgcggcgaac
     1081 tcattccttt cacttttcgt gaagagaggc ctgggcagct caaactctcg aagctccgcg
     1141 gcgagcggca ggagtacgtc agcgcccccc ttgcccttgg ccatctggtc aagcccatcg
     1201 cccaacgtga tcagggtgtc gagagaaaca aggcgctggt cgtccatcac ctggtgcatc
     1261 cgcctggcca tctgcaatcg cagctgttgc ccttctttgt tgtactgctc tggacccgcg
     1321 agcacggaaa atagctggtc ctgcgaaatg gatgtctttc cggtcgcggc gcgcaaaagc
     1381 tccgcgaatg aagctcgccc ggcttcgtat acctgttcgg acgaatgcaa tgtgctgaac
     1441 ggaatcaata ctttttccca tgaatcgttc agttgcgcgt ctggaatttc gccctggcgg
     1501 gcaagtattt cccagaggcc cacattggct tgcatcattc cgagcgcgtc ggtgcgggca
     1561 agacgatcgg gaatttgatt gagtccttct gctactttca gaaagcgcgt gatggagccg
     1621 ttgttcaatg cgtgaaattc cgcgaacctt ggatactggt ccccgaaaag tggaaatttt
     1681 tcggcaagca atcgcgctgt ggccgtattc aattgttcgc cgggggcacg attgcgatca
     1741 atttcggtaa gcattaaaaa attgtagacc ggtccgtcgg gcatgggata ccgcgttacc
     1801 gcaaccatgc cttcgacgac ttgctccgga ttgttccagc tgctcgcttt atctccccac
     1861 tcgcgaacaa gtttcgaatc aaacttgcgg tgcaaaactt ccttccagac ttcaatgccg
     1921 ccgggaatgt gcggctggcc actcggatct aacattaaac gcgcttccaa gaggaacagg
     1981 cccggatcgg gacggaacga atgcttggtg ggtagcgggt tgaggtcatg tccgcgaagc
     2041 gcttcgtaca tgcgaacgag ccgacgagat gacgtgaaat agccctgctg cgttgggccc
     2101 acgcgcgaaa gagtgtcaaa ataagaagcc gcccacccgc catccttaat cagcaacttc
     2161 gaaatgaatt ccgcaggaga gcgcggattc gcgccaacta ggtccctcca ggcagactcg
     2221 ctggaggctc cgcccggcac gaggacgcgg ccggaacgaa cgctgagctg gctgccataa
     2281 aaatccaaca cgggagcgac caagatcagt ttcttaaatc ccatcgtctg ccacaataat
     2341 tgggcggtct ccgagtccat gcgggacatc gcccagtaaa gtcgggcgac attgggatcc
     2401 cgcaagaggg catcgaccac tcctcgattg gaattttttt tggctggtac ccagtccgaa
     2461 gttttataca acacgggaac ttttgttggt ccatatggaa tatcgatcgg cttcccttcc
     2521 cgcaatgaat cttccaaatc cgctaaaggg aatccggaat taacggtgag gaacgcgcgg
     2581 ttggcgtctg cggtctccaa agccgcgtca ggtccgcagg atttttgcag tcggtagccc
     2641 agaacggaga gcaacggctt agcgtcgtcg caatttgaga catgcaggac ctgggccttg
     2701 ccggcgagcg cttccagttc acgggcctga tcgagatacc aattcaagag gactagaaat
     2761 tgggtgggtc tgccgacctc gtaaccgctc atcaccacat tgcgcgcaag cagcggcaac
     2821 agctcgtcag gcgtgatctg ctggctgatg gctgccatac ggagaaacga acgcatcggc
     2881 ccggggatgg acgcgacgtc agcaggcttg gcatccttgg atgatgttct ctgaggggga
     2941 cttgcgggcg aggcggccaa aggcgtggcg agcaagcaca ttatcacgaa tttagggaaa
     3001 gcacagaagc cgcgatgaaa tctcatggcc caaacctccg ctgacatcca aggacgttgt
     3061 tcttagatcg tgacgacgaa gagaatttgg gggatgtttc tctccgcaag gcccccgtgg
     3121 gaacattttt cggccgccga ttacgaccgt tcgcttcctg accgaatccg aatttcgtct
     3181 tcgaagcgag ctgcgaacag gacccgttcg cctcgatgca tcattcctag tgtgcggaga
     3241 ataaaccatt cctcatggcc cagtcaatcc gcgtgggtct ggataagtct gccttccaga
     3301 attcagaaaa taagagcagg ccgtgcacca tacttagaaa aaacataacc aaatttctat
     3361 cggtatttca cctgatttgt tgaattggga tcaggattaa ttaactaaaa gtatcggcaa
     3421 agttgcatcc gggaagttgc ctcgtacgaa cttgaaattt gcttgtcacc gcgcgcggga
     3481 atgctatgct ggcgctcatg aaaatcgcat ttcttggact gggaaaaatg ggcgcgggca
     3541 tggcgcgcaa tttaatgcgt gcgggtcaca cgctggctgt ctataaccgc agccggggaa
     3601 aggcggaggc cctggccgca gagggagcgc gcatcgccga ttcgccagcg gatgccgcga
     3661 gggacgcaga cgccgtgatg agcatggtgg cggacgatca cgcgctggaa gctgtgatct
     3721 ttggggaaca cggcgctgct tcggccatga aggaagggtg cgttcacctc tcgcatagca
     3781 ccattagcac cgcgatggcg cgaaggctga cttcggaaca cgcgcaacgt aaacaagtct
     3841 ttctgagcgt acccgtgttt gggcgtccgg acgcggcgga aaacaagaat cttgtggtga
     3901 tggttgcggg ccctgcggag cttgtggaac ggtgcaggcc gctgtttgac gcgattgggc
     3961 gcgtgacatt cgtcgttggc gccgaaccgt ggcaggcgaa tgtggccaag gtctgcggaa
     4021 attttatggt gatcagcgcg attgaggcgc tgggcgaggc ttacgcaacg ctgcggaagg
     4081 cgggcgtcga ccctgagatt tttcttgatg tgatgcattc cctgttcggc tcaaacgtga
     4141 tcactggcta cgggcgcatc atcgcaaagg aacaattcga gccggcgggg ttcgcgttga
     4201 aactgggact taaagatgtg cgtctggtgc tggcggcgtc cgaggagtgt gcttcgccga
     4261 tgccgctggc cagtttggta cacgatcatt tactggcggg tctcgcgcag ggacagggtg
     4321 aaatggattg gtccagcatg acgcaggtga ttgctcggaa tgcggggttg aaggcgaaaa
     4381 gctagtgggg tgagttcgct ttgagatcct gctgacatga atactttcta ctttcaatcg
     4441 taaaaactgc ctgcccctcg ttgctcgggg caagcggaag ccgacgctac aaaggagatt
     4501 tgaaaatgtc tcttgtggat ttcattgcca cgcctaagtt tgaggaatat cgggaacggt
     4561 tcaagcagca ctaccgaatg gagcgcagca aggatggcgt gattgtggtg gaagcgcaca
     4621 cggtgggcgg gccgattcag ctgagcgtgg aaaatcatcg ctcggtgggg cagcttttta
     4681 aaaccattgg cgcggatccg gaaaatgaag tgatgatttt tacgggttca ggcaaagact
     4741 tcatgatgga cgcggacccc gagggtttta aactggagca ggaagattta cagcattggg
     4801 cgtacgaata tgcttacaaa gacgggcgca tcaa
//
LOCUS       DASZMB010000215        10030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_5157907, whole genome shotgun sequence.
ACCESSION   DASZMB010000215 DASZMB010000000
VERSION     DASZMB010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 10030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10030
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5157907"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VG272_10090"
     CDS             complement(<1..576)
                     /locus_tag="VG272_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HWF14073.1"
                     /translation="MSQPISLPGVTASSIDGELLWGFWYPALRSNELRGRALVTSTRM
                     EIPLVLGRDAAGKAFALRDVCPHRAFPLSAGSCDGRTVECTYHGWRFDAHTGQCREIP
                     SITVDSKLQCDRIYAGSFPCKERDGYIWVYLANAEGRATRLPDGQMDRSAIPPVPALP
                     KFSERYRIAHLTADLMCNMDTAITGLMDPAHG"
     gene            735..1187
                     /locus_tag="VG272_10095"
     CDS             735..1187
                     /locus_tag="VG272_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14074.1"
                     /translation="MKRWVLGIFQLVVASTMAPQVFFFGVPGLLGSLGMSGSGHFDAD
                     LSGVPKLYFWSLLVASFSLVIAMILAFAGAVLTLARPSQFAVRACMAGAFFYWIFIVG
                     SLLISFALGIHFSFRYMTVWDVMQYPIGIAAVIVAYALYSQTKARGVS"
     gene            1397..2488
                     /gene="prfA"
                     /locus_tag="VG272_10100"
     CDS             1397..2488
                     /gene="prfA"
                     /locus_tag="VG272_10100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HWF14075.1"
                     /translation="MAGLTLYEKLDQIEHRYVEMTTELSSPEILNDSANYQKLARRHS
                     ELSEMVEKYREWKQVDKELTGAKQMVQEADDAEMKQMAEEEEKQLAERKESVERELKI
                     LLLPKDPNDEKNVIVEIRAGTGGDEAALFAGELFRMYSRYAESQKWKVEILESSPSSL
                     GGMKQIVASIQGQKVYSKLKYESGVHRVQRVPATEQQGRIHTSAATVAVLPEADDIDI
                     KIEAKDLRIDTFCSSGPGGQSVNTTYSAVRITHIPSGLVVSQQDEKSQIKNRAKAMRV
                     LRARLYELEQSKQHEAMAAERLGQIKTGDRSEKIRTYNFPQNRVTDHRIGLTLHQLTE
                     VMDGKLGPFVDALVTHFEAERLKQELVEA"
     gene            2594..3529
                     /gene="prmC"
                     /locus_tag="VG272_10105"
     CDS             2594..3529
                     /gene="prmC"
                     /locus_tag="VG272_10105"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HWF14076.1"
                     /translation="MNSHFSPAMNRLSLFATLRDGIAQLERKSVPSAGLAAELLLMHV
                     IGQDRAWVYAHPEAELDSAQREQYLTLIARRASGAPTQHLTGHQEFWGLDFEVTPDVL
                     IPRPETEHLIEVALERLGITKEAGAPRRDEKFRIADVGTGSGCIAISLAHELPTASIA
                     ATDISAAALKIAGRNAARYGVSDRIEFAECNLLDEFFQSRSTSHQSSQFDLIVSNPPY
                     IGRWEASSLQREVREHEPEAALYGGETGTEIYAPLIAQAAARLKPGGVMVLELGHTSS
                     AHVSKLLDSNEWTSVTISNDLAGIPRVASAVRKQN"
     gene            3807..4406
                     /locus_tag="VG272_10110"
     CDS             3807..4406
                     /locus_tag="VG272_10110"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HWF14077.1"
                     /translation="MDELKNPWTILTQSTVYENDWIRVEHHDVLGPLGGAGIYGTVHF
                     KHQATGVVPIDDQGNVILVGQYRFPLNTYSWEIPEGGGSHSATALESAQRELREECGV
                     AAASWKEILVMDLSNSVSDEGCTAFLAWELSEVPSEPDETEKLQVTRVPFWDAVDRAK
                     QGEIRDAISVAALTRVALMALQDELPEDVAKVIGWRRKS"
     gene            complement(4403..4732)
                     /locus_tag="VG272_10115"
     CDS             complement(4403..4732)
                     /locus_tag="VG272_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14078.1"
                     /translation="MFQSSILRINDQGTAEERLWRAVITKSLEEWICGPLSFSRKAEQ
                     FLFDDNKDFKAVCSSAGMDPERLRKRLTKLRARGIQKLNIPFRVRTNKRLAFSQNGSI
                     GQSAFGV"
     gene            complement(5066..5614)
                     /locus_tag="VG272_10120"
     CDS             complement(5066..5614)
                     /locus_tag="VG272_10120"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWF14079.1"
                     /translation="MGKIHQTIDDSIREFIDAQPLFFVATAPLDSTSHVNVSPKGLDT
                     LRVLGPTAVAYLDLTGSGIETVSHLKENGRIALMFCAFQGPPKILRLHGRGKVVETGE
                     TDFVDLAVHFPEQEGKRAIIVIEVTRISDSCGYSVPLLQYQGERDQLTAWSKRLGSEG
                     LKKYRKEKNQRSIDQIPSLEKP"
     gene            5714..7006
                     /gene="dinB"
                     /locus_tag="VG272_10125"
     CDS             5714..7006
                     /gene="dinB"
                     /locus_tag="VG272_10125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HWF14080.1"
                     /translation="MPKFVTYRAKRDSDAGPAPSGSSTSTTSSPSSTSSICSTSILHV
                     DMDAFFVSVELLDRPELRGKPVVVGGQPDQRGIVSTASYEARKYGIHSAMPLRMAGRL
                     CPHAIFLEPRHHLYSEWSDRVAAILGKYSPVVEMASVDEAYIDLAGTERLHGPPIAAA
                     DALLREITATTGLPCSAGLARTRLVAKVASDQAKPRGLVWIPAESEEAFLAPLDVRRI
                     PGIGKATEAALQSAGIQKVGQLAAMGREKLEKEFGRWGTALFRKAHGGDTYEFFVDAE
                     PKSISHNRTFSEDTQDRALLDATLSLLCQKAMKRLREASLSAGVVTVTIRYMGFETIT
                     RAHTLREPTHLDPIVYETAKKLFDRHCDRTRKVRLVGLALSSFTHGGEQLDLLDASRR
                     EKLERIAQATDALRDRFGFSKIQFGGSMTKSRKEEQED"
     gene            7293..7718
                     /locus_tag="VG272_10130"
     CDS             7293..7718
                     /locus_tag="VG272_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14081.1"
                     /translation="MTSSNAVLRNPNPHFGTLGVLWIVYGVIRLIMAACLLIYASTAT
                     LMFGALLNRVADPFTLMGLFHFLYTAIIALSVLCGLLAIFAGLALLGERSVGRKLAVV
                     ASVLSLCDIPLGITLGTCTLVELLPIQTAPLYDRSGQTA"
     gene            7941..9374
                     /locus_tag="VG272_10135"
     CDS             7941..9374
                     /locus_tag="VG272_10135"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HWF14082.1"
                     /translation="MTPRELAERICGTLRGHGHQAYFVGGCVRDLELGREPADYDVCT
                     DARPEQVQELFPRSVAVGAKFGVILVLGDEAPKVGVQRNEGAEVVSEVETETVAGIAA
                     ENPAQQNRTQVEVATFRSDGGYSDGRHPDQVIYSRTPQEDVLRRDFTINGLLMEPATG
                     EILDYVGGRADLRMGIVRAIGDPRLRFSEDKLRMLRATRFAARFGFQIEPETMAAAQS
                     LAPQITQVSYERIRDELTKLLTEGAARRGFELLDQTGLLAVVLPDIARMKGVEQPLQF
                     HPEGDVWIHTLMLLEKLPPNCSPTLAWGVLLHDVGKPPTFSPATGPGTRIRFNGHEEI
                     GARMAENICRSLRFSNEETAQIESLVANHMRFKDVPKMRAATLKRFVRLPRFDEHLEL
                     HRLDCLASHGGLESYTFVQRFLLETPVEKVYPPKLVTGDDLKGLGLVPGPRFKEILTA
                     VEEAQLEGRLHDRESALEFVRSVSQVQ"
     gene            9745..>10030
                     /locus_tag="VG272_10140"
     CDS             9745..>10030
                     /locus_tag="VG272_10140"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HWF14083.1"
                     /translation="MSEKSIQIPKELPILPVRDTVLFPGGVQPLTVGREGSLALLNSL
                     HGDEKLLGIVAQHDPRVEEPAAADLHLVGTAAKIHKLVRMPNGNVVVFLEG"
BASE COUNT         2270 a         2526 c         2817 g         2417 t
ORIGIN      
        1 tccgtgtgcc ggatccatca gacctgtgat ggccgtgtcc atgttgcaca tgaggtcggc
       61 ggtgagatgc gcaatgcgat agcgctcgct gaatttcggc agcgcgggaa cgggcgggat
      121 cgccgatcga tccatctggc cgtcgggcag gcgtgtggcg cggccttcgg cattcgccag
      181 atacacccaa atgtagccgt ctcgttcttt gcacgggaaa ctgccagcgt agatgcggtc
      241 gcattgcagc ttggagtcca cggtgatcga aggaatttcg cggcactgtc cggtgtgcgc
      301 gtcaaatcgc cagccatggt atgtgcattc cacggtccgg ccgtcgcaag agccggcgga
      361 aagcggaaat gcgcggtgtg ggcaaacatc gcgcaatgca aacgcttttc cggcggcatc
      421 gcgtcctaac acgagtggaa tttccattcg cgtcgaagtc acgagtgcac gaccgcgaag
      481 ttcattgctg cgcagcgcgg gataccaaaa gccccacagc agttcaccgt caatgcttga
      541 cgcagtcaca cccggcaggg aaataggttg gctcatgttc atgtgtattc gcttgcctat
      601 gatataccga ttgcttgtga ccgcaatgtc gcgcgccctt tattgcgaga aatgttttgc
      661 aaaagcatga tcatcgaaac tgcttgcgta tgtgttggac cgcgaggaag atgcacgtgt
      721 tataaaacta aaatatgaaa cgctgggttc tggggatttt tcagcttgtt gttgcgtcga
      781 ccatggcgcc gcaagttttc ttcttcggag ttcccgggct attgggttca ctgggaatga
      841 gcgggagcgg gcattttgac gcggatttgt caggtgtgcc gaaactttat ttctggagtt
      901 tgctggtggc gtcgttttct ctggtgattg ccatgattct tgctttcgcc ggcgctgtgc
      961 tcacgcttgc gcgaccttcc caattcgccg ttcgagcgtg catggcggga gcatttttct
     1021 actggatatt tatcgtcggg tcgctactga tttcgttcgc gttaggaatt catttcagtt
     1081 ttcgttacat gaccgtgtgg gacgtcatgc aatatccgat cgggatagcg gccgtgattg
     1141 tggcttacgc actttattcc caaaccaaag ctcgtggagt ttcatgacca ctcggaatgc
     1201 tctctttgac cacgactcta ttttcgcttc attgcatttg cagcaatttc acgctatttt
     1261 attacatata aaccgtgttc accgcattct attagccgca accccttctg gatccactcc
     1321 gttggatcgc cgaaccatac tgttttgata gacttagtaa ttactcccga ttggaattct
     1381 acttgagggg caagccatgg cagggttaac gctgtatgaa aaacttgacc agattgaaca
     1441 ccgctacgtc gaaatgacca cggagctttc ttcgccggaa attctgaatg actcggcgaa
     1501 ttaccagaag cttgcccggc gtcactcgga actttccgag atggtcgaga aataccgcga
     1561 gtggaaacaa gtggataaag aactcacagg cgcgaagcag atggtccagg aagccgacga
     1621 cgccgagatg aagcagatgg ccgaggagga agagaagcaa ctggccgagc gcaaggaatc
     1681 cgtggagcgc gagctgaaaa ttttgctgct gcccaaggac ccgaacgacg aaaagaacgt
     1741 gatcgtcgaa attcgcgcgg gcacaggcgg cgacgaggct gcgttatttg cgggtgaact
     1801 gtttcgcatg tactcgcgtt acgcggaatc gcagaagtgg aaagtggaaa tcctggagag
     1861 ttcgccgagt tcgcttggcg gcatgaaaca gatagtcgct tcgatccagg ggcaaaaagt
     1921 ttactccaag ctgaaatatg aaagcggcgt gcaccgcgtg cagcgcgtcc cggccaccga
     1981 gcagcaggga cgtattcaca cttcagccgc gaccgtggcg gtgttgcccg aagcggacga
     2041 tatcgacatc aagattgaag cgaaggatct gcgcatcgat actttttgct cttctggacc
     2101 gggtgggcaa tcggtgaaca cgacgtattc cgcggtgcgc atcacgcaca ttccatccgg
     2161 cctcgtcgtt tcgcagcagg atgaaaaatc gcaaattaaa aatcgtgcca aagccatgcg
     2221 cgtgttgcgc gcgcgcttgt atgaattgga gcagagcaag cagcatgaag ccatggccgc
     2281 cgagcgcctc ggacaaatca agacgggcga tcgctcggaa aaaatccgca cttacaattt
     2341 tccgcaaaat cgcgtcaccg atcatcgcat cggcctgacc ttgcaccagt tgacggaagt
     2401 gatggatggc aagctgggcc ccttcgttga cgcgctcgtc acgcattttg aagccgagcg
     2461 acttaaacag gaattagtcg aggcctgaaa gtagcgcggg cttcagcctg cgggatttag
     2521 tccttggaag ggccaacccc cgcaggcctg aagcccgcgc cactgaattc gcacttttga
     2581 ttgaagctgc aatatgaact cccacttttc accagctatg aatcgattgt ctctatttgc
     2641 cacccttcgt gacggcatcg cacaactgga gcgcaagagc gtgccatcgg ccggactcgc
     2701 tgcggaattg ctgctgatgc acgtcatcgg gcaggaccgc gcgtgggttt acgcgcatcc
     2761 ggaagcggaa ctcgattccg cacaacgcga gcaatatttg acgctgatcg cacggcgcgc
     2821 aagtggggcg ccgacacaac atctcacagg gcatcaggaa ttctggggac tcgattttga
     2881 agttactccc gacgtactta ttccgcgccc tgaaaccgag catttgatcg aagttgcgct
     2941 ggagcgtttg ggaataacga aggaagctgg tgcgccgcgt cgcgatgaaa aatttcgcat
     3001 cgcggacgtg ggcacgggtt cgggctgtat tgcaatttcg ctggcgcatg aattgccgac
     3061 tgcgagcatt gctgcaactg atatttctgc tgcggccttg aaaatcgctg gtcgcaacgc
     3121 cgcgcgctat ggagtttcag accggattga attcgccgaa tgcaatttgc tggatgagtt
     3181 tttccaatca cgatccacta gtcaccagtc gtcgcagttc gatctcatcg tgagcaatcc
     3241 gccgtatatc gggcgctggg aagcatcgtc tcttcaacgc gaggtccgcg agcatgagcc
     3301 tgaagcggcg ctctatggcg gtgaaacggg gacggaaatt tatgcgccgc tcattgctca
     3361 agccgcagcg cggctgaaac caggcggcgt tatggtgttg gagcttgggc acacgtcctc
     3421 tgcccatgtt tccaagctgc ttgattccaa cgagtggact agcgtgacga tttccaatga
     3481 tctcgcggga attcctcgtg ttgcgtccgc agtgcgcaag cagaactaaa ttttcatgga
     3541 acgatggttt ggtttacgta acataggttt cagcctgtgg aatttaggcc ttgcatggac
     3601 caacccgcca caggctgaag agtctgtgtg agaactgcaa aattggaact tcagcggcta
     3661 aagccgcact cattttgcag accttatgtc gcggctaaag ccacgaccca caatgattct
     3721 gcagttctca cacagactct gaagcctatg ctgcttaaaa ccaattcctt cttttctggg
     3781 cgagtatgtt tttgagtgga gaaaaaatgg acgagttaaa aaatccttgg acgattttga
     3841 cgcagtcaac ggtttacgaa aacgattgga ttcgcgtgga gcatcacgat gtcctggggc
     3901 cgctcggcgg ggcgggcatt tatgggaccg tacatttcaa gcatcaggcc acgggagttg
     3961 tcccgattga cgatcaagga aacgtaattc tggtggggca gtatcgattt ccattaaaca
     4021 cttacagttg ggaaatacct gagggtggtg gctcccattc ggccaccgca ctcgaatcgg
     4081 cgcagcggga actgcgcgag gagtgcggtg tggcagcggc aagctggaaa gaaattttag
     4141 tgatggatct ttccaacagc gtttccgatg aaggctgcac ggcgttcctg gcgtgggaac
     4201 tttcggaagt gccgtcggaa ccggacgaaa cggaaaagct tcaagtgacg cgcgtgcctt
     4261 tctgggatgc tgtggatcgc gccaagcaag gagaaatccg cgacgcaatc agcgtcgcgg
     4321 ctctcactcg tgttgctttg atggcgctgc aggatgaact tccggaagat gttgccaaag
     4381 tgattgggtg gcgtcgaaaa agttaaactc caaatgcgct ttgtccgatg ctgccgttct
     4441 gggaaaaagc caatcgcttg tttgttcgta cgcggaaggg aatgttcaat ttttgaatgc
     4501 cgcgggcccg taacttcgtc agccgctttc gcaggcgttc cggatccatt ccggccgagg
     4561 agcacaccgc tttgaaatct ttgttgtcgt caaaaagaaa ttgttccgcc ttgcggctga
     4621 aactcaacgg tccgcaaatc cattcttcca ggctctttgt aatcacagcg cgccaaagtc
     4681 tttcttccgc cgtaccttga tcattgatac gcaagattga gctttgaaac atggttttgg
     4741 cccccagttc aatttgtttt gttcggctga gtgacctcct aagtgcaggt aagggaccac
     4801 atgtggctta acgccaatga tagataaaaa acaaaatgac gaattttggc gtagtggaac
     4861 ggaatgttct taatacgggt tttacggtat gcgggctctt gtcgttttag tcactaaaaa
     4921 ctatggctga caaaatatgg cgtttcttcg agagcataaa tccgcgaaga aaaatataaa
     4981 aatttcggaa ttttttcccg cgggatagag attttacaag gccgccgatg gagggaacgg
     5041 cgagtgtcat tcttccatat tatttttacg gtttttcgag gcttggaatt tggtcgatgc
     5101 ttctttgatt tttttccttg cggtacttct tcaatccttc cgaccccaat ctttttgacc
     5161 aggcggtcag ctgatcgcgc tcgccttgat attggagtaa gggaacgctg tatccgcagg
     5221 aatcggaaat tcgtgtgacc tcgatcacta tgatcgcccg ctttccctct tgttcaggaa
     5281 aatgaaccgc aagatcgacg aaatctgttt cgccagtttc tacaactttg ccgcgcccgt
     5341 gtaggcgaag aatttttggc ggcccctgga acgcgcagaa cattaacgcg atgcggccgt
     5401 tttctttgag atgagagacg gtctcgattc cgctgccggt cagatccaga taggcaacag
     5461 ccgtggggcc caagacgcga agtgtgtcga gccccttggg agagacattg acgtgactcg
     5521 tggaatcgag cggcgccgtc gctacaaaaa agaggggctg ggcatcgata aattccctga
     5581 tggaatcgtc aatcgtttga tggattttcc ccacagccag gcgctcctta ttcttaatcc
     5641 taacatgttt ttgaaaaaga aatgttcgcc tcttattcgc ctgggtgtat agtacctgtg
     5701 aagttttcaa aatatgccaa agtttgtcac ttaccgggcg aagcgggatt ctgacgcggg
     5761 gcccgcgcct tctgggtcct caacatccac aacttcttca ccatcctcta cttcctccat
     5821 ttgttctact tctattctcc atgtcgacat ggatgcattc ttcgtttccg tcgaattgct
     5881 ggaccggccg gagctgcgcg ggaagcccgt cgttgttgga gggcagccgg atcagcgggg
     5941 cattgtttcc acggcgagct acgaagcgcg aaaatatgga attcattcgg cgatgcctct
     6001 gcgcatggcg ggtcgcctgt gtccgcatgc aatttttctt gaaccgcggc accatcttta
     6061 ttccgagtgg agcgatcgcg ttgctgcgat tctcggaaaa tattcgccgg ttgtagagat
     6121 ggcatctgtc gatgaggcct acatcgacct tgcggggacg gagcggctgc acgggccgcc
     6181 aattgccgct gcggatgcgt tgctgcgcga aataacggcg accactggat tgccgtgttc
     6241 cgccggtctt gcgcgaacgc gccttgttgc aaaagttgcg agcgatcagg caaagccacg
     6301 aggcctggtg tggattcctg ccgaaagcga ggaagcattt ctcgcgccac tcgatgtgcg
     6361 gcgcattccc ggaatcggaa aagctacgga agctgctctg caatccgcag gcattcaaaa
     6421 agtcggtcag cttgctgcga tggggcgcga aaaactggaa aaagaattcg gccgctgggg
     6481 cacggcgctc tttagaaaag cgcacggcgg cgacacgtat gaattctttg tggacgccga
     6541 gccgaaatcc atttcgcaca atcgcacatt ttcggaagac acgcaggacc gtgcgctgct
     6601 cgatgccacg cttagcctgc tgtgccagaa agcgatgaag cggctgcgcg aagcgagtct
     6661 aagcgcggga gttgtcacag tcaccatccg ctacatggga tttgaaacta tcacgcgcgc
     6721 gcacactttg cgcgaaccga cacatcttga tcccatcgtg tatgaaaccg cgaagaaatt
     6781 attcgatcgc cattgcgacc gcactcgaaa ggtgaggctc gttggattgg cgttgtcttc
     6841 cttcactcat ggtggcgaac agctggacct gctcgacgca tcacgccgtg aaaaattgga
     6901 gcgcatcgct caagccaccg acgctttgcg cgaccgcttc ggcttctcaa agattcagtt
     6961 tggcggctcg atgacaaaat cgcgcaaaga agaacaggaa gactaaagtc atcggtcgga
     7021 taaactactt tctaactaat tacattcttt cagttcttac acgatttact taccctgccg
     7081 ttttcgcagc atggatgggc atctgaatag attgtttacc cgatggaatt caatcgcccc
     7141 gtccggtact ttcccattag cacctttatt gcgggcctca accatctacc tgttcagatg
     7201 tgaacagtat ttcctcttgc accgagttat aaaagattgt tcatgcgctg atatcgatac
     7261 tcggcttgag agaaagctca aaggaggctg ttatgacgtc aagcaatgct gttctccgga
     7321 atccaaaccc tcatttcgga actctaggag tcctttggat cgtttacgga gtgatacgtc
     7381 taatcatggc ggcctgcctg ctgatttatg ccagcacagc tacgttgatg tttggagcgc
     7441 ttctgaaccg tgtcgctgat ccctttactt tgatgggact ttttcatttc ttgtatacgg
     7501 ccatcattgc attgtcagtt ctgtgtggcc ttcttgcgat tttcgctggg ctcgctctac
     7561 ttggagaacg gagtgtcggc cggaaacttg ctgtcgtcgc atccgttttg tcgctttgcg
     7621 atattccgct gggcataact ctgggaactt gcacactagt ggagttgctc cctatccaga
     7681 cagcgccgct ttacgatcgt tccgggcaga cggcctaatt tgcatttctc acatggaacc
     7741 cggcgctacc aaagcgaggc ttgtgggcgt cattgttcag atgttttgac cgcgtttggt
     7801 cgtgagaaaa gcggattgat ttgtcccgac caacgaacac agaaaactaa ttggcagttt
     7861 gatgacgaaa tcacgcaggt tgaaagaaaa ttcgagtcac actcctttgc tcatcgctca
     7921 gactggcgca taatccagaa atgacaccgc gcgaattggc tgaacgaatc tgcggaacgc
     7981 tgcgaggcca cgggcatcag gcctacttcg tgggcgggtg tgtgcgcgat ctggaattgg
     8041 ggcgcgagcc tgcggactat gatgtatgca cggatgcgcg gccggagcag gtgcaggaac
     8101 tttttccgcg cagcgttgcg gtgggcgcga aattcggcgt gattcttgtc ctcggggatg
     8161 aggcgccaaa agtaggagtg cagcgaaacg aaggggcgga agttgtgtcg gaggttgaga
     8221 cggaaactgt ggcgggaatc gcggcggaaa acccggcaca acaaaacaga acgcaagtag
     8281 aggtcgcgac gttccgctct gacggtggct actcggatgg acggcatccg gaccaggtga
     8341 tttattccag gacgccgcag gaagatgttc tccggcgcga tttcacgatt aatggattgc
     8401 tgatggaacc ggcgacggga gaaatccttg actatgtcgg cgggcgcgcg gatttgcgta
     8461 tggggattgt gcgcgcgatt ggcgatccgc ggctgcgttt ttcggaagac aagctgcgca
     8521 tgctccgcgc tacgcggttc gcggcgcgat tcggtttcca gattgagccg gaaacgatgg
     8581 cggcagccca gagcttggcg ccgcaaatca cgcaggtttc ctacgagcgc attcgcgatg
     8641 agctaaccaa gcttctgacc gaaggcgcag cgcggcgtgg attcgagcta ctggaccaaa
     8701 cgggcttgct ggccgttgtg ctcccagaca tcgcgcggat gaagggtgtg gagcagccgc
     8761 tgcagtttca tcctgaaggg gatgtgtgga tccacacgct catgctgctc gaaaagcttc
     8821 caccgaactg ctcgccaacg ctggcttggg gagtcctgct gcatgatgtg ggcaagccgc
     8881 ccacattttc gcccgccacc ggcccgggga ctcgcatccg cttcaatggc catgaagaga
     8941 tcggtgcgcg catggctgag aatatctgcc gcagcctacg cttttcaaat gaagagacgg
     9001 cgcagattga atccctagtg gcgaatcaca tgcggttcaa ggacgtgcca aaaatgcggg
     9061 cggcgacgct taagcggttt gtccgcctcc cgcgatttga cgaacatctg gagctgcatc
     9121 ggcttgactg tctggcaagt cacggcggcc tcgaatccta taccttcgtc cagcgctttc
     9181 ttttggaaac tccggttgaa aaagtatatc cccccaagct ggtcacagga gatgatctca
     9241 agggcttggg cctagtaccg ggaccgcgat tcaaggaaat cctgacggcg gtagaagagg
     9301 ctcagcttga aggacggctc cacgaccggg agagtgcact cgaatttgtc cggtcagtca
     9361 gccaagtgca gtaaaaacga aaaagtacct gagacccata gagattccag cgaaagccgg
     9421 atgctatttg tggttacggg tcttacgggt ttagacagct cacgcggtta atgaacttgc
     9481 cgatatgagc tttatgccct gaaaagggct agtactctta aatacaagat cgaagctttg
     9541 cgattaataa gttgtttatt tatagtaatt taatggaatt aaaactttat ttttaaatgg
     9601 acgtctaact ctttagtggc atgcactaaa ctttgttgct cctgtctttg tatctttcgc
     9661 gtatcctttg ggtctgtcct gcatctaaat gaaatggtcg agtgctgaag aagttgctgg
     9721 acttaggaat tcaaggggga cctaatgagt gaaaagtcga tacaaatacc caaagagctg
     9781 ccaattctgc ctgtgcggga tacggtgctg tttccaggag gcgtgcaacc cctaaccgta
     9841 ggcagagagg gttctctagc cctgctgaac tcgctccacg gagatgaaaa actgctagga
     9901 attgtggcgc agcatgatcc tcgtgtggag gagccggctg ccgctgacct gcatctggtg
     9961 ggtacggccg ccaaaatcca caaattggtg cgcatgccaa atgggaatgt cgtcgtgttc
    10021 ctggaaggat
//
LOCUS       DASZMB010000216         3404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_5158794, whole genome shotgun sequence.
ACCESSION   DASZMB010000216 DASZMB010000000
VERSION     DASZMB010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5158794"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="VG272_10145"
     CDS             <1..1103
                     /locus_tag="VG272_10145"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWF14084.1"
                     /translation="KQEIALADKTYSDNKKQVDVGTLAPLEIVQAEAQLATAQQALIV
                     AQTTVLQDQLTLLNLISKDPNSPVLRTVEIVPTDKADVAPPEVEKLPLEDLIREAITK
                     RPDVVQSTLVIKGDDVNVQATRNALLPILTLNGEYATEGLGGNTRLQTSCTPVPPAVT
                     CVIPPQVFSGLPTALNQTFTNVYPEYNASINLTIPIRNRFAQANNVIATLEKRSDEAN
                     YQQIVNNAVTDVHNAQITLEQARITLDAAIKTRDLDQQTLDAEQKKYQVGASTLFNIV
                     SDQNTLAAAESAEVRARVNLVEGKVNFDRAMARTLEVYNISIADARSGKTTKDTLIPG
                     TAVNGQLFVDPAKQANASASTANRPAGNTNAQ"
     gene            1455..3095
                     /locus_tag="VG272_10150"
     CDS             1455..3095
                     /locus_tag="VG272_10150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HWF14085.1"
                     /translation="MASERPPSPENTIGDKPAKNARDFTTISGHPIRELYTPADLSSW
                     NAEKDLGLPGEPPYTRGIHPAMYRSRLWTMRQFAGFGSAQDTNQRFRYLLSQGQAGLS
                     VAFDLPTLMGYDSDHSLAEGEVGKCGVAISSLADMEALFDQIPLGNVSTSMTINSPAA
                     ILWAMYLAVAEKQGVDWKNLGGTLQNDILKEYIAQKEYIYPPKPSMRLVIDTFEFASR
                     NAPKFNPISISGYHIREAGSTAIQELAFTLRDGIEYVEWGVQRGLAVDDFALTLSFFF
                     NAHNDFFEEIAKYRAARRIWYKTMSERFGAKLARSSAMRFHAQTAGCSLTAQQPYNNV
                     VRTAVQALAAVMGGTQSLHTNSLDEAWALPTEFAATLALRTQQILAHETGVAKTVDPF
                     GGSYFVETLTNEIEEAAWKYIRKIDEMGGMIPAIERGYPQREIADASYKYQQAVDRKE
                     KIIVGVNEFKGEEKTIDVLRIDESVRTQQSERLRKLRVERSSDEVARQLVALRKAAQG
                     TENLMPHIFSAVKAYATLGEICDALREVFGTHEEASIT"
     gene            3191..>3404
                     /locus_tag="VG272_10155"
     CDS             3191..>3404
                     /locus_tag="VG272_10155"
                     /inference="COORDINATES: protein motif:HMM:NF024759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HWF14086.1"
                     /translation="MTTNEITREIIGAAIEVHKRLGPGLLESAYEECLLHDLHLRNLR
                     LERQKGLPIVYKELITPSTKYVTRFSE"
BASE COUNT          852 a         1018 c          830 g          704 t
ORIGIN      
        1 caaagcagga aattgcgctc gcggataaaa cttatagcga caataaaaag caggttgacg
       61 ttggcactct cgctccgctg gaaatcgttc aggccgaggc gcaactcgct accgctcagc
      121 aagcattgat cgtcgcgcaa accacggttc tgcaggacca gctcacactc ttgaacctga
      181 tctccaagga tccgaattct ccagttttgc gcaccgtcga aattgtccca acggacaagg
      241 cggacgtggc gcccccagag gttgaaaaac ttcccttgga agacttgatc cgggaggcaa
      301 tcaccaagcg tcccgacgtt gtacagtcaa cccttgtaat taaaggagat gacgtcaacg
      361 ttcaggcaac acgcaacgcg ttgcttccta ttttgaccct gaatggcgaa tatgcaacgg
      421 aagggctcgg aggcaatacc cgcttgcaaa ccagctgcac ccctgtgccg cctgcggtaa
      481 cttgtgtcat ccctccgcag gtattttcag gactgcctac agctctgaac caaaccttca
      541 cgaatgtata tcccgaatat aacgcttcca taaatttgac gattccgatt cgcaatcgtt
      601 tcgcgcaggc caataacgtg attgcgacgc tggaaaagcg ttcggacgag gccaactacc
      661 agcaaatcgt aaataacgct gtcaccgacg tgcacaacgc acagatcacg ctggagcagg
      721 cgcgcatcac cctggacgcc gcgatcaaga cgcgcgatct cgatcagcaa accctcgacg
      781 ccgagcagaa aaaatatcag gttggcgctt ccactctgtt caatatcgtg tccgatcaaa
      841 atacattggc tgcggcggaa tccgcggaag tccgcgcgcg cgtcaatctg gtggaaggaa
      901 aagtaaattt tgatcgcgcc atggcccgca cgttagaggt gtacaacatt tccattgccg
      961 acgcccgatc cggaaagaca accaaagaca cgctaatccc cggcactgcc gtcaacggcc
     1021 aattgtttgt cgatccggcg aaacaggcga atgcgtctgc cagcaccgca aatcgtcccg
     1081 cgggaaacac aaacgcccag taaatttatg aacggccggg accgttattg tgacagtgaa
     1141 aaaatcccca gttctgatcc ctcattttca ctcgggacca gcgacccggg ctacaactgc
     1201 cccggaaaat ctcgaatcac ttgcactcga aaattgtgtt cgcctgctgc acccggctgc
     1261 tatagcccgc ctctcttgtc ctgagtgaaa cgagggatca gatgcggggc ttttctgcaa
     1321 ccttcgccca acacaatcga cggtttccgt tcaaccttcg cgccctcact tgtcctgcca
     1381 cacccattgc cctaaaatca aattgcgcac aggcgcggcc cggccaaatt agaaaccggc
     1441 ctgacgcata acccatggca tcagagcgcc caccgtcacc agaaaataca ataggcgata
     1501 agccagctaa gaacgcacgc gacttcacca ccatttccgg acatccgatt cgcgagcttt
     1561 atactcccgc tgacctttcc tcttggaacg cggaaaaaga tttgggtctc cccggtgaac
     1621 ctccctacac gcgcggcatt caccccgcga tgtatcgcag ccgattgtgg acgatgcgcc
     1681 agttcgccgg cttcggctcc gcgcaagata ccaaccagcg cttccgatat ttgctttcgc
     1741 agggacaggc agggctctcc gtcgctttcg atttgccaac cttgatgggt tacgactccg
     1801 accattcgct tgccgagggc gaagtcggaa agtgcggcgt tgcaattagc tcgctggctg
     1861 acatggaagc gctcttcgat caaattcctt taggaaatgt ttccacgtcg atgaccatta
     1921 attcgcccgc cgcaattttg tgggccatgt atttagccgt tgcggagaag cagggcgtgg
     1981 actggaaaaa tctcggggga actttacaga acgacattct caaggaatac atcgcgcaga
     2041 aagaatatat ttacccaccg aaaccttcga tgcggctggt gatcgacaca tttgaatttg
     2101 cctcgcgcaa cgcgccgaaa ttcaatccca tttccatcag cggctatcac attcgtgaag
     2161 cgggatcgac cgcgattcaa gagctggcgt ttacgctgcg cgacggaatc gagtatgtcg
     2221 aatggggcgt gcaacgcggt ctcgccgtcg acgatttcgc gctaacgctt tcctttttct
     2281 tcaacgcaca caacgatttc ttcgaggaaa tcgccaagta tagggccgcg cgccgcatct
     2341 ggtacaaaac gatgtctgag cgctttggag ccaaactggc gcgatcctcg gccatgcgtt
     2401 ttcatgcgca gaccgccgga tgctcgctca cggcgcaaca accctacaac aacgttgtgc
     2461 gaaccgccgt gcaagcgctc gcggccgtca tgggcggcac gcaatccctg cacacgaatt
     2521 cgctcgatga agcctgggcg ctgcccacgg aatttgccgc gacgctcgcg ctacgcacgc
     2581 agcaaatcct cgctcatgaa acaggcgtcg cgaaaacagt cgatcctttc ggcggctcgt
     2641 atttcgtcga aacgctaacc aacgagatag aagaggctgc ctggaaatac attcgcaaga
     2701 ttgacgagat gggcggcatg atcccagcca tcgaacgcgg ttacccgcag cgcgagattg
     2761 ccgacgcgtc ttacaagtat cagcaggcgg tagaccgcaa agaaaaaatt atcgtcggcg
     2821 taaatgaatt taagggagaa gaaaaaacca tcgatgttct gcgaatcgat gaatcggtcc
     2881 gcacgcaaca atccgaacgc ttacgtaaac tgcgcgtcga gcgatccagc gacgaagtcg
     2941 cgcgccaact cgtagctcta cgcaaagccg cccagggcac cgaaaattta atgccccaca
     3001 tcttctccgc cgtaaaggcc tatgccacgc tcggagaaat ctgcgacgca ctgcgcgaag
     3061 tcttcggcac gcacgaagaa gcctccataa cgtaagcttt taccttaagc cctccttcca
     3121 actctccgct tttctcctct tttctctgtg ccctctgtgt taaattcccc tttcccagaa
     3181 gtcaccgcct atgacaacaa acgagatcac gcgcgaaatc ataggagctg caattgaagt
     3241 tcacaagcgc ttgggtccgg gcctgctgga atccgcatac gaagaatgcc tcctgcatga
     3301 ccttcacctg cgcaacttga ggctagaacg tcagaaaggt ctcccgatcg tctacaagga
     3361 acttattact ccgagcacta aatatgtgac acgtttcagc gagc
//
LOCUS       DASZMB010000217         4982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3517006, whole genome shotgun sequence.
ACCESSION   DASZMB010000217 DASZMB010000000
VERSION     DASZMB010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3517006"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..905
                     /locus_tag="VG272_10160"
     CDS             102..905
                     /locus_tag="VG272_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HWF14087.1"
                     /translation="MAPFLENAKQVAIISALAEGSSIRSIEQITGVHRDTIMRLGVRV
                     GQGCAELLDAKMRNLSCEHLQFDELWGFIGKKQKHVQPEDSRELGDVWTFCAIDADTK
                     LVPSFKVGKRDMVTANAFVSDVASRMRNRIQVSTDGLPGYVEAVERAFGKDVDYAQVI
                     KVYESEALDAEVIRVDKRTFAGRPDLDLTTTSHVERLNGTTRLHMRRLTRLTYAFSKK
                     FENFEAAVALHFAYYNFVKRHNTLRATPAMAAGIEHDFWTVGNLVEAAV"
     gene            973..1212
                     /locus_tag="VG272_10165"
     CDS             973..1212
                     /locus_tag="VG272_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14088.1"
                     /translation="MPVKETFQGKTIWEGIVEVFELKGHPQTERVYAWSHDTDDPKKP
                     KRHVTVLHIAPILSAEAAVKAAIVQEFRNRETEEA"
     gene            1541..2905
                     /locus_tag="VG272_10170"
     CDS             1541..2905
                     /locus_tag="VG272_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HWF14089.1"
                     /translation="MAIATINPATGQTVKTFEELTSAQVDQKIQRAVEAFPKFRKLSF
                     AERGKMMLRAAELLESEKNALAKLMTLEMGKTLRSAVDEAVKCAWVCRYYAENAEKFL
                     ADEVVETPASRSFIRYQPMGAVLVIMPWNYPFWQAFRFIAPGLMAGNVGLLKHASNVP
                     QCALKIEELLSSAGVPEGVFQTLLIGSGAVDRVLADPRIAAATLTGSEGAGVKVGAGA
                     AQRIKKVVLELGGSDPYIVMPSANLDEAIATGVKARVANNGQSCIAAKRFIVHEQIAD
                     KFQEAFVAQMKALKVGDPFDEKTDVGPLANAEAVKSLDADVQSTIKSGAKVLTGGKPV
                     DGPGSFYMPTVLTDIPNNSPAYAEELFGPVASIFRVKSIDEAIRVANDSRFGLGASAW
                     TNDKAEQEKFMNELEAGMVFINQMVFSDPRVPFGGVKRSGVGRELSTYGIREFTNVKT
                     VWVK"
     gene            3047..3760
                     /locus_tag="VG272_10175"
     CDS             3047..3760
                     /locus_tag="VG272_10175"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HWF14090.1"
                     /translation="MYKVIQSSRLYEQIVQQIEDSILKGELTEGSQLPAERELAEQFG
                     VSRTAVREAIKALQEKGLVDAFPGRGTFVTNGTSNSMRRSLDRIIKTSERDGWTYLVE
                     VREILEPEIAALAASRADLEDLEAMRECINAMDSAERDSDAFVEADLDFHLALAEAAA
                     NPIVLSLIDSIVGLLREQRVRISLVEGGPERGQRYHRSILEAIERQDPQAAKAAMQAH
                     LWQVREDSRNTPGSQPAGS"
     gene            3791..4672
                     /locus_tag="VG272_10180"
     CDS             3791..4672
                     /locus_tag="VG272_10180"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWF14091.1"
                     /translation="MANLGFVGLGVMGGNMVARLLDKGHTVTGYNRTRTKAQWLIDRG
                     MKWGDSPRAVAAAADFTFSMVTNSAAINSITEGPDGILAGLAPGKLLIDMSTVSPAVS
                     RALAAKVREKGADMIDSPVSGSVITLQEGKLSIMVGGKRETFERAKPVLLDVGPKVTH
                     VGDNGLALAMKIATNLSLAVQMLAFCEGVLLAEKSGIARETAVDVLTHSVIASPMVQY
                     RGPFVLKMPDEAWFDVNMMQKDMLLALEMGRSLDVPLPTTAVTNEFLTASRGMGLAKQ
                     DFAIMFEALARMSGVSK"
     gene            4669..>4982
                     /locus_tag="VG272_10185"
     CDS             4669..>4982
                     /locus_tag="VG272_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWF14092.1"
                     /translation="MKTESKQPATVDGSGSELLLRMYSNMQRIRLFEEQVNQLYTRAV
                     MPGLAHLYIGEEAVAVGICEALHRDDYITSTHRGHGHCLAKGASPDRMFAELLGKEAG
                     YC"
BASE COUNT         1296 a         1259 c         1417 g         1010 t
ORIGIN      
        1 aaataatcga ccgtatcata gtggcaatac tgtaccacaa caatatatgt tgacaagcac
       61 cacttattgg ggtacagtaa taggcataaa ggagatcgaa catggcccct ttcctagaaa
      121 acgcaaaaca agtggcaatc atcagcgcac tagcagaagg gtctagtatt cgctctattg
      181 agcaaatcac aggtgttcac cgcgacacga ttatgcggct tggcgttcgc gtcggacagg
      241 gatgcgcgga attgctggat gccaagatgc gaaacctctc ttgcgagcac ctacagtttg
      301 acgaattgtg gggatttatt ggcaagaaac agaagcacgt tcaacccgag gatagccgcg
      361 aattgggaga tgtttggacc ttctgcgcga ttgacgcgga cacaaaactt gttccctcgt
      421 tcaaagtcgg caagcgcgat atggtgacgg caaatgcgtt cgtgagcgat gtggcgtcac
      481 gaatgaggaa tcgcattcaa gtatccacag acggccttcc gggatatgta gaagcggtag
      541 agcgagcatt cggcaaggat gtggattacg cgcaggtcat caaggtctat gagagcgaag
      601 ccctcgatgc ggaagttatt cgtgtggaca agagaacctt tgctggccgt cccgatctgg
      661 accttaccac gactagtcac gtcgagcgat tgaatggcac gacccgcctt cacatgcgcc
      721 gtttaactcg cctgacttac gctttcagca agaaatttga aaattttgaa gcggcggttg
      781 cgctccactt cgcctattac aatttcgtaa agcgccacaa tacattgcgg gctaccccgg
      841 caatggcagc gggaatcgaa cacgacttct ggacagttgg gaatctcgtt gaggcagccg
      901 tgtgaactac atagacgatc taaaaaagtg attaaacggt cacacggagt agaggccaag
      961 cacatcgaga gtgtgcccgt caaagaaaca tttcagggca agacgatttg ggaaggcatc
     1021 gttgaggtat ttgagctaaa aggccatcca cagaccgaga gagtctatgc gtggtcgcat
     1081 gatacagacg atcctaagaa gccgaaacgt catgttaccg tgcttcatat tgcgccgatt
     1141 ttatcggcag aggcagcggt caaagcggcg atcgttcagg agtttagaaa tcgtgagaca
     1201 gaagaagcct aaaaaagccg gacgcccacc actcccgaag ggcagcgcaa aagcaggtac
     1261 actccgcgtg cgtgtaacgc cggacgaact gaggagcatt gaatcaaagg cgaagacgaa
     1321 taaacagact gtttcggagt ggattagggg cacgttaaat gccgcaattc aataggcaag
     1381 acccgctatt aggacactac cgaaaatccc ttccacaggc aaacgcgcgg aaaacgtggt
     1441 acaacaataa gatcgcgtat acgaaatatt ttcgtggcgc tccggctatc tcatgattcg
     1501 gaatgacatt atattttcct cgactagggc ggataaaatt atggccatcg caacaatcaa
     1561 tccagcaacg ggacaaacgg taaagacctt tgaagaactc acgagcgcgc aggttgacca
     1621 gaaaattcag cgcgcggtgg aagcattccc gaaatttcgc aaattatctt ttgccgagcg
     1681 cggaaaaatg atgctgcgcg ccgcggaact tctggaaagt gaaaaaaatg ctctcgccaa
     1741 gctgatgacg ctggaaatgg gcaagactct gcgctccgcc gtcgacgaag ccgtgaagtg
     1801 cgcctgggtc tgccgttact acgccgagaa tgcagaaaaa ttcctggccg atgaggtcgt
     1861 agaaacaccc gcgagccgaa gttttattcg ctaccagcct atgggcgcag tgctcgtgat
     1921 catgccttgg aattatccat tctggcaggc gttccgtttt atcgctccgg gactgatggc
     1981 gggcaacgtt ggcctgttga aacacgcgtc gaatgttccg caatgcgcgc taaaaattga
     2041 agagcttctc tcgagcgcgg gcgtgcccga aggcgttttc caaaccctgc tgatcgggtc
     2101 tggtgcggtg gacagagtgc tcgccgatcc gcgcatcgcc gctgccacgt tgacgggcag
     2161 cgagggcgcg ggagtaaaag tgggagcagg cgccgcgcaa cgcatcaaga aagtcgtgct
     2221 cgagctcgga gggagcgatc cttacatcgt gatgcccagc gcgaacctcg atgaagccat
     2281 agccacaggc gtcaaggcgc gcgtggccaa caacggacaa tcctgtatcg ccgccaagcg
     2341 attcattgtt cacgaacaga ttgccgataa atttcaggaa gcgtttgtcg cgcaaatgaa
     2401 agccctgaag gtgggcgatc cgttcgacga aaaaacggat gtcggtccgc tggccaatgc
     2461 cgaagcggta aaatcacttg atgcggacgt gcagagcacg attaagtctg gcgcaaaagt
     2521 attgaccggc ggaaagcccg tggatggccc cggaagcttc tatatgccca cagtgctcac
     2581 cgatattccg aacaattctc cggcctatgc cgaagaattg ttcgggcctg tcgcctcgat
     2641 cttccgcgtg aaaagtattg acgaggcgat tcgcgtggcg aatgacagcc gcttcggcct
     2701 cggagccagc gcctggacga atgacaaagc cgagcaggaa aaattcatga atgaactcga
     2761 agccggcatg gttttcatca atcaaatggt attttccgac ccgcgtgttc cctttggtgg
     2821 cgtgaagcgg tccggggtgg gccgcgaact cagcacgtat ggcatccgag aattcacgaa
     2881 cgtaaagaca gtctgggtga agtaagtcga ggaatctttc tacgtagcaa aatatcactt
     2941 gcatccataa aaaaccaatg gtatggtggt cataccatta ggtgagccgt gcattgctac
     3001 acttttaaat ctaaggccgc ctgaaggaag aagcgaggcc taactcgtgt acaaggtcat
     3061 ccaatcgtcg cgcctttacg agcaaatcgt tcagcagatc gaggactcca tcctcaaggg
     3121 cgaattgaca gaaggcagcc aactccccgc cgagcgcgaa ctagccgagc aattcggcgt
     3181 cagcagaaca gcagttcgcg aagccatcaa ggcattgcag gaaaaagggt tagtcgacgc
     3241 gtttccaggc cgtggtacgt ttgtgaccaa tggaacctca aattccatgc gccgatcgct
     3301 ggaccggatc atcaagacaa gtgagcggga cggctggacc tacctcgttg aagtccgcga
     3361 aattctggaa cctgaaatag cagcgctagc tgcctctcgc gctgacttag aagatctcga
     3421 ggccatgcga gagtgcatca atgcgatgga tagcgcggaa cgggattccg atgctttcgt
     3481 cgaagccgac ctcgattttc atttggccct ggcagaagcc gcggctaatc cgattgtcct
     3541 ttcactgatc gattccattg ttggattgct gcgcgaacag cgcgtgagaa tttcgctcgt
     3601 cgagggcggc ccggaacgcg ggcagcgcta ccacagaagc attctcgaag cgatcgagcg
     3661 gcaggatccg caggccgcaa aggcagccat gcaagctcat ctttggcaag tacgtgaaga
     3721 ttcacgcaac actccgggta gtcagccggc gggatcctaa acacaatttc gaaattggag
     3781 aattcacgcg atggcaaatc ttggtttcgt aggactcgga gtgatgggtg gcaacatggt
     3841 cgcccggctg ctcgacaaag ggcatacggt cactggctac aatcgaacgc gcacaaaagc
     3901 acaatggctc attgaccgcg gaatgaagtg gggggactcg cctcgcgccg tcgccgcggc
     3961 ggcggatttt acgttttcga tggtcacgaa ttccgccgca atcaattcaa ttacggaagg
     4021 acctgacgga atcctcgcag gacttgctcc cggcaaactc ttgatcgaca tgagcacggt
     4081 cagccctgcc gtgagtcgcg cgttggcagc caaagttcga gagaagggcg cggacatgat
     4141 cgactcgccc gtctccggca gcgtaattac gctgcaagaa ggaaaactat cgatcatggt
     4201 tggcggcaag cgagaaacat ttgagcgcgc caagcctgtg cttctcgatg tcggtccaaa
     4261 agtaactcac gtgggagata acggccttgc actggcgatg aaaatcgcca ccaacttgag
     4321 cctggccgtg cagatgctcg cgttttgcga aggcgttctg ctggcggaga aaagcggaat
     4381 cgctcgcgaa actgccgtag acgtgctgac gcacagcgtg atcgcatcac caatggttca
     4441 atatcgtggc ccttttgtgt taaaaatgcc ggatgaagca tggttcgacg tcaacatgat
     4501 gcagaaggat atgctgttgg cgttggaaat ggggagatcg ctggacgtgc ctttgccaac
     4561 cacggccgtg acgaatgaat ttctgaccgc atcgcgaggc atgggcctgg cgaagcaaga
     4621 ctttgccatc atgtttgagg cgttagctcg catgtcaggg gtaagcaaat gaaaacagaa
     4681 agtaaacaac ctgcgacggt cgatggatcc gggagcgaat tgttgttgcg aatgtacagc
     4741 aacatgcaaa ggatccggct gttcgaagag caagtgaatc agctatacac gcgcgcggtg
     4801 atgccgggcc ttgcgcacct ttacatcggc gaggaagccg tcgccgttgg aatttgcgaa
     4861 gcgctgcatc gcgatgacta catcacaagc acgcatcgcg gacacggcca ctgcctggca
     4921 aagggcgcgt cgccggaccg catgttcgcc gaactgctgg gaaaagaagc gggatactgc
     4981 aa
//
LOCUS       DASZMB010000218         4766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_213406, whole genome shotgun sequence.
ACCESSION   DASZMB010000218 DASZMB010000000
VERSION     DASZMB010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213406"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..595
                     /locus_tag="VG272_10190"
     CDS             185..595
                     /locus_tag="VG272_10190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWF14093.1"
                     /translation="MRSDELLNPRDAARSLGISYPTLKQWIYKKKLRSVQTAGGHHRI
                     PASEIERFLPRTEEKDTLAGRRNFRRISGRNQLVGRILEVRYSGLLAQVKLSIGGQRI
                     SSIITADAAREMRLEKGQTAAALIKSTEVMIVRV"
     gene            612..1457
                     /gene="modA"
                     /locus_tag="VG272_10195"
     CDS             612..1457
                     /gene="modA"
                     /locus_tag="VG272_10195"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HWF14094.1"
                     /translation="MKRTHSGILFAEVMGLVLLSPIFISRTNARETASQAGVKSCGTI
                     TIAAASDLTYAMNEIAANFEKVTGCSVHMSMGSSGNFLTQIENGAPFDLFFSADIAYP
                     KKLEMEGLAAAGSTYLYAIGKIVLWTRNDSHVDISNGLPALRDLAVRKIAIANPQHAP
                     YGRAAEEALRNAGVYDSVKDRLVLGENISQAAEFVESGNADAGILALSLVLSPSMKGE
                     GRTSTIPGSLYKPIEQGAVVLLASKNQHGAKQFLEYIKLPSTVALLERYGFTLPANIS
                     TGGKH"
     gene            1454..2128
                     /gene="modB"
                     /locus_tag="VG272_10200"
     CDS             1454..2128
                     /gene="modB"
                     /locus_tag="VG272_10200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HWF14095.1"
                     /translation="MNWQAVLLSLKLAICVAGILLAVGMPIAYWITFSRWRLKFLVEA
                     IVALPIILPPTVLGYYILVGLGPRHFAGKLWQSLFGHTLPFTFGGLLIASVIYSLPFA
                     VQPFSAGFETVDRRVIEAAWTLGASRLSTFFRVILPLSVAGAVTGVVLSFAHTIGEFG
                     VVLMVGGNIPGVTRTVSIDIYDQVQAANYNAADQTAVILLLFSFFVLALIYGTNRRAL
                     SLWRRF"
     gene            2338..3423
                     /locus_tag="VG272_10205"
     CDS             2338..3423
                     /locus_tag="VG272_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HWF14096.1"
                     /translation="MSLEVAIEKNVDGFRLAVEFMADGAPLGLLGPSGSGKTMTLRAI
                     AGLETPDRGRISLDGNVLFDSERKINLSARERRIGLLFQNYALFPHLTAAENIAFGLQ
                     GVSEADRKRRVAEQLVAAQLIGVAERYPATLSGGEQQRVALARALAIEPAALLLDEPF
                     SALDTHLRGALERQLRETLATYRGSTLFVSHNLEEAYRVCEKIVVLANGKVAAQGPKE
                     EIFRHPPTIEVARVTGCKNYSRARRMPDGCIEAMDWGCKLSVAQQFAKPPEHVAIRAH
                     HIRVHPPEYFVAKNFRKNIFPCWLAAMTETPFRVTLDLRIGLAPANSNDFHVQAEVFK
                     QEWESFRDLPQPWAMELAPDRLFLLPD"
     gene            complement(3654..>4766)
                     /locus_tag="VG272_10210"
     CDS             complement(3654..>4766)
                     /locus_tag="VG272_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14097.1"
                     /translation="LTVNWNGSPRPTTFVSSTQLTATISAADTAFLATTTSPAAVAIT
                     VSGSNPATNSNSLPFFVTTLPTGTTLPAPVINSILPTSAATEAQTGPAIALTVNGNGL
                     SPCTIAQWNGNPLPATIFIGSGGVASFIPAANLTATGTNQITAVTPGPGVSNSEPFAV
                     FTPGSPTVTNVPGGSLSLPLLSASQRFGVFVLASPDGTTETPGTTQNIFLKDTCLGVP
                     SGCTASIALVSAATNSTPGAPVPGNADSISPSISASSAPGGPDGRYVAFLSSATNLVT
                     PATNGLMQAFVRDTCAGVASGCTPLTQIVSVSTGGTQANAPVLSATIDATGRYVTFES
                     SATNLGSTTSSSIGLFVRDTCTGAAPGCTPTTQPLN"
BASE COUNT         1044 a         1171 c         1412 g         1139 t
ORIGIN      
        1 cctaaaccaa tcgtacaaga actaaactcc atcgtcctgc aagctgacgg cggcgcggcg
       61 aggaatccga aggtaagggc ctgattcatg cacagagaat atcaaattag aggatttgag
      121 taaagtgaat aatcttgtac ataactgata aatacgcgta taaatgtatt ttctggaggt
      181 gtccgtgcgt tccgacgaat tgctcaatcc gcgagacgct gcgcgttcgc tggggatcag
      241 ttatcccacg ctgaagcagt ggatttataa aaagaaattg cgaagcgtcc agactgcggg
      301 aggacatcac cgcattccgg cgtcggaaat cgagcgcttt ctaccgcgca cggaagaaaa
      361 agacacgctg gcgggccgcc gcaattttcg ccgcatcagc gggcgcaatc aattggttgg
      421 tcgaatcctc gaagtgcgct acagcggctt gctggcgcag gtgaaacttt cgataggcgg
      481 gcagcggatt tcctcgatca tcacggcgga cgcggcgcgc gaaatgcgcc tggaaaaagg
      541 gcagaccgca gcagcgttga ttaaatccac agaagtcatg atcgtgcgcg tgtgagcccg
      601 gagacgcctc gatgaaacgc acacattctg gaattttgtt tgccgaagtt atgggactcg
      661 ttctcctttc gcctattttt atttcccgga cgaatgcacg cgaaactgcg tcgcaagctg
      721 gggtgaaatc atgcggcacg ataacgattg cggcggcttc cgatctgacg tacgcaatga
      781 atgaaattgc cgcaaacttt gagaaagtta caggatgttc ggtgcatatg agcatgggtt
      841 cttccggaaa ttttcttaca caaattgaaa atggggcgcc attcgactta tttttctccg
      901 ccgacatcgc ctacccgaaa aaactggaaa tggagggcct ggctgcggcg ggcagcacgt
      961 acctgtatgc tatcggcaaa attgttttgt ggacgagaaa cgattctcac gtggatatca
     1021 gtaacggcct gcctgcgttg cgcgaccttg ctgtgcgcaa aattgcaatt gccaatccgc
     1081 aacacgcgcc gtatggacgc gctgctgagg aagcgctgcg caacgccggc gtatacgaca
     1141 gcgtaaaaga ccgcctggtt ttgggcgaaa atatttcgca ggcggcagag tttgtcgaat
     1201 ccggaaatgc ggatgccggt atcctggcgc tatcacttgt cctttcacct tcgatgaagg
     1261 gcgaaggccg cacgtcgacg attcccggaa gtttatacaa gccgattgaa cagggcgcgg
     1321 tcgtattgct tgcatcgaag aaccaacacg gcgcgaaaca atttctcgag tacatcaagc
     1381 tgccttctac cgtcgcactg cttgagcgct acggttttac tctccccgca aatatttcta
     1441 ctggggggaa gcattgaact ggcaagcggt tcttctttcc ctgaagctgg cgatttgcgt
     1501 ggcgggaatt ctgctcgctg tgggcatgcc cattgcgtac tggattactt tttcgcggtg
     1561 gcgcctgaaa tttttagtgg aagcgattgt cgcgttgccc atcattttgc cgccaacggt
     1621 tcttggctac tacattcttg tgggtttggg tccgcggcat ttcgcgggaa aactttggca
     1681 atcgttgttt gggcacacgt tgccatttac gtttggcggt ttgctgattg catccgtgat
     1741 ctacagcttg ccgttcgccg tgcaaccgtt ttcggctgga tttgaaaccg tggaccgccg
     1801 ggtgattgaa gcggcctgga cattgggtgc ctcacgtttg tccacgttct ttcgggtaat
     1861 tttgccgctc tctgttgccg gtgctgtcac tggcgttgta ttgagcttcg cgcatacgat
     1921 cggcgaattc ggcgtggtgc tcatggttgg gggaaatatt cctggagtta ctcgcactgt
     1981 ttccatcgac atttacgatc aggtacaggc ggcgaattac aatgcggcgg atcagacggc
     2041 tgtgattctg ctgctgtttt cttttttcgt gttggcgctg atctatggaa cgaatcggcg
     2101 agcgttgagt ttgtggagga ggttttaatc tggcataggc tttagagtct gtgtgagaac
     2161 tgcaaaatgg aaacttcagc ggctaaagcc gcactcattt tgcaggactt atgtcgtggc
     2221 taaagccacg acccacaacg attctgcagt tctcacacag actcttagcc tgtgggtttt
     2281 ttgtccttgc aaggactgcc ccccgcagcc tgaaacctgt gccactgaaa ccaaattatg
     2341 tcactggaag ttgcgattga aaaaaatgtg gatgggtttc ggctcgcggt tgaatttatg
     2401 gctgacgggg cgccgctcgg cttgcttggt ccctcgggtt cgggcaagac catgacgctg
     2461 cgcgcgatcg cggggcttga aacgccggat cgcgggcgaa tctcgttgga tgggaatgtg
     2521 ctgttcgatt ccgaacgcaa aattaattta tccgcgcgcg aacgccgcat cggtttgctt
     2581 tttcaaaatt atgcgctgtt tccgcattta acggccgcgg aaaatatagc gtttggattg
     2641 cagggagttt ccgaggcgga tagaaagcga cgcgtcgccg aacagcttgt cgctgcgcaa
     2701 ctcataggcg tcgcggaacg gtatccggcg acgctctccg gcggcgaaca gcaacgcgtt
     2761 gcgctggcac gcgcgctagc gattgagcct gctgccttac ttctcgatga gccgttttca
     2821 gcgctcgaca cgcatttgcg cggcgctctg gaacgtcaac ttcgtgaaac gctggcaacc
     2881 tatcgcggct cgaccttatt cgtaagccat aatctggaag aagcttaccg cgtttgcgaa
     2941 aaaatagtcg tgctggcgaa cggcaaagta gcggcgcagg ggccgaagga agaaattttt
     3001 cgccaccctc caacgatcga ggtcgcgcgc gttaccggat gcaagaatta ttcgcgggcg
     3061 cgcaggatgc ctgatggctg cattgaggcg atggattggg gctgcaagct gagcgtcgca
     3121 cagcaatttg cgaaacctcc tgaacacgtg gcgattcgcg cgcaccacat ccgtgttcat
     3181 ccgccggagt attttgtggc gaaaaatttt cgtaagaata tttttccctg ctggttggcg
     3241 gcgatgaccg agacgccttt ccgggtcacg ctcgatttgc gaattggcct ggcacccgca
     3301 aattcaaatg attttcacgt gcaggcggaa gtgttcaagc aggagtggga atcgtttcgc
     3361 gatctaccgc agccgtgggc gatggagttg gccccggacc gattgttttt gttgccggat
     3421 taattaggga tggatttaag tgggacaggc ttcagagtct gtgtgagaac tacagaatct
     3481 ttgtgggtcg tggctttagc cacgacataa gtcctgcaaa atgaatgcgg ctttagccgc
     3541 tgaagcttca attttgcagt tttcacacag accctgaagc ctgtctgctt ttgaattttt
     3601 gctggtgtgg caaaagtcaa aagaaaacag gctgaagcct gttctactta aagctaattc
     3661 aacggctgcg ttgtgggcgt gcagcctggt gctgctcccg tgcaggtatc ccttacaaat
     3721 aaaccaatgg aagaactggt tgtcgagccc aggtttgtgg ccgaggactc gaacgttacg
     3781 tagcggcctg tggcgtcgat cgttgccgat agaacggggg cgtttgcttg cgtcccgccc
     3841 gtggagacgg aaacgatttg cgtcaaaggc gtgcagccgg acgcaactcc tgcgcacgtg
     3901 tcgcggacaa atgcctgcat gagcccgttg gttgcgggcg tcacgaggtt tgtggcggaa
     3961 gaaagaaatg ccacgtagcg tccatcggga ccgcccggcg cgctactcgc actgatggat
     4021 ggcgagatgc tgtccgcatt acccggaacc ggagcgccag gagtgctatt ggtcgcagca
     4081 gaaacaagag cgatggaagc cgtgcagccg gacggtactc ccaggcaagt atccttcagg
     4141 aaaatatttt gcgtagttcc gggagtttct gttgtgccgt cgggagaagc gagtacgaac
     4201 acgccgaagc gctggctcgc actcagcaag ggcaaagata acgatccacc aggaacgttt
     4261 gtgacggtcg gcgatcccgg tgtgaatacg gcaaacggtt cactgtttga aactcccggt
     4321 ccgggcgtga ctgcggtgat ctggttcgtt ccggtggcag tgagattagc cgcaggaatg
     4381 aaggaagcga ctccgccaga accgatgaat atggttgcgg gcagaggatt gccattccat
     4441 tgggcaatcg tgcagggcga caagccattt ccatttacgg tcagtgcaat tgcgggcccg
     4501 gtctgagctt cggtagccgc acttgtcggt aaaatcgaat tgatgacggg cgcgggcaga
     4561 gtcgtgcccg taggtaacgt tgtcacgaaa aaggggagcg agttcgaatt cgtcgctgga
     4621 ttcgagccgg ataccgtgat agccaccgct gccgggcttg tagttgtagc gaggaatgct
     4681 gtgtccgcag ccgaaattgt ggcggtgagc tgcgtagagc tcacaaaagt cgtagggcgc
     4741 ggcgagccat tccaattgac agtgag
//
LOCUS       DASZMB010000219        33969 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_518040, whole genome shotgun sequence.
ACCESSION   DASZMB010000219 DASZMB010000000
VERSION     DASZMB010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 33969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33969
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518040"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VG272_10215"
     CDS             <1..422
                     /locus_tag="VG272_10215"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWF14098.1"
                     /translation="VDWTAARQAHARVYDSLLKDLPGVLIPRVPLGREHVYYLYTIRV
                     EGRAGGNTRSNDRGNEVRSDLVQQRLKGRGIGSTVYYPVPLHLQPLYASLGGKPGELR
                     VSERASREVLSIPIYPELTPEQVERVAQEVRKATQED"
     gene            complement(587..811)
                     /locus_tag="VG272_10220"
     CDS             complement(587..811)
                     /locus_tag="VG272_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14099.1"
                     /translation="MRIKFALAALLAMTVSAQGAFAQGCALCYTTASAMGVSAQRSLD
                     LGILALVSPALILFLSVIFMLYRRAVSATA"
     gene            complement(856..1179)
                     /locus_tag="VG272_10225"
     CDS             complement(856..1179)
                     /locus_tag="VG272_10225"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HWF14100.1"
                     /translation="MNTHAHAEEIHAGASVGTNLAIWVGLVAITGLEVFLAYKQLQPS
                     IMLSILVVLSVVKAAMIMSYFMHLKFEKLSLVLWLVPAMIFCICMMFIFFFPEAFRLL
                     QMRPH"
     gene            complement(1208..1840)
                     /locus_tag="VG272_10230"
     CDS             complement(1208..1840)
                     /locus_tag="VG272_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HWF14101.1"
                     /translation="MLEVKNDVALDSAWNGGKSPFAIGSKKLGMWLFIVSDTLTFSAL
                     LIAYSYSRITNPDWPRPFPIYPAIAFSTTMTVVLLASSLTMVMAVMAAQRNDRKKTVM
                     WMVATMIGGLLFTGLHLTEWFNMITKEHFTITGNPEGTPLFGAVFFGITGLHMTHVMI
                     GVIYLGIVAVGYGRGKFSAEDVEVNGLYWHFVDLVWMFVFPLIYLMSVKY"
     gene            complement(1844..2497)
                     /locus_tag="VG272_10235"
     CDS             complement(1844..2497)
                     /locus_tag="VG272_10235"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HWF14102.1"
                     /translation="MPGTVFDDVEIIDADHGGGTPPPAGGDGDGDSHGSRRPFPRRAY
                     FTALQLGLAAIVMFFMAMASSYIVRRGLGTDWQRTPIPPVLWFNTAVLLISSVTIVVA
                     RKKLASGEREAFQSLWWVTTGLGVLFLAGQVIAWRQLAAAGMLLATNPSSSFFYVLTA
                     AHGAHLIGGIFALFYVAFRPWRKSRISQATAAELTSIYWHFMDGLWIFLLALLTLGR"
     gene            complement(2516..4288)
                     /locus_tag="VG272_10240"
     CDS             complement(2516..4288)
                     /locus_tag="VG272_10240"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HWF14103.1"
                     /translation="MVTHENGGHAHHAPTGFIRKYVFSIDHKVIGIQYLCLALFSAFV
                     GMGLSLLMRLHLGWPAAKFAFFKGGQMTPEQYLALMTMHGTIMVFMVLTTAPQSGYGN
                     YFLPIQIGAADMAFPVLNMLSFWTTFVSLVVMMCAFLVSGGAPIGGWTSYPPLSALGE
                     IVGPGQGAGETLWIIGLAIFCGASLMGALNFITTLIDLRAKGMTLGRLPLTCWTWFVT
                     AILGLLAFGVLLAACILLLLDRVAGTSFFVPGGLVVTDQVITWHKGGSPLLWQHLFWF
                     FGHPEVYIAILPGMGVASHLLSTFSRKPVFGYRAMVGAVCGIGFLGFCVWGHHMFISG
                     MSPYSAFAFSILTMTIGVPSAIKTFNWLGTLWGGKITFNCAMLFALGFVSLFVTGGLS
                     GLFLAQPALDLYLHDGYFVVAHFHLIMGVAAIFGMFAGLYFWFPKMFGRFMNEGVGKV
                     HFWFTFIGVYTVFMPMHFLGLAGNPRRYSEMTAGPFLQQLQPLHRFISVAAFITVGAQ
                     LIFLFNFFWSLLAGEKATEENPWHATTLEWSIPSPPPFDNFGGVEPVVYHGAYEFSVP
                     GAADDYIPQHLAPELIRKSEGSTE"
     gene            complement(4395..5147)
                     /gene="coxB"
                     /locus_tag="VG272_10245"
     CDS             complement(4395..5147)
                     /gene="coxB"
                     /locus_tag="VG272_10245"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HWF14104.1"
                     /translation="MTIYIFMAKIWWFPPAITDFGHEIDSQFARTFLITGIVFVAAQL
                     GLAIAIFKFRDHGQKATYFEGNNTMEIVWTLATVVLFVGLGLYARNAWAEVHFMGAQP
                     GALQIEVTGQQFVWNFRYAGPDGKFGREKPELVSASTGNPVGLDPTDPASKDDIVSPV
                     IVVPAGKEVELIIRSQDVTHSFYVRELRLKQDAVPGMEIHMHFNATEPGDYEIACAEL
                     CGLGHYRMHSMLTVMPPADFDKWAKEQEAANQ"
     gene            5468..5998
                     /locus_tag="VG272_10250"
     CDS             5468..5998
                     /locus_tag="VG272_10250"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HWF14105.1"
                     /translation="MRKHSAFLVIALAALATCAIPVAAQVLSPPAKAACKFADGKMIS
                     VNYSSPRMRGRKIFGDLVPFGEVWRVGADDATSFVPNADVTVGGKNIPAGRYTIFALP
                     TPNRWTLIVSKQIGEWGIPYPGAQFDFARMEMKVSKLSAPLENFTISFDAPGSACTMK
                     FDWETTRASIDISEKK"
     gene            6202..7536
                     /locus_tag="VG272_10255"
     CDS             6202..7536
                     /locus_tag="VG272_10255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF14106.1"
                     /translation="MTTAGSRQTIPGKTASVPGRHIAAVVVGNALEFYDFLTYAFFAV
                     YIGRTFFPSSIPSASLLASLGTFGVGFVTRPIGAWVIGRMGDRMGRKPAMVFSIALTG
                     VAITGLALTPSHARIGVVAPILVIFFRLLQGFALGGEVGPTTAFLLEAAPPERRGFYA
                     SFQLWSQHLSVLASGLVGFGLANLLSEQQLQDYGWRIAFLVGVAIVPLGLVMRRSLPE
                     TFHAPSSGSASEPASLRPHIGIAVLGVMLLASTTIGNYIDNYMTTYAITTLHMRVNIA
                     FAATIIVGLSGVTFDLISGALSDRIGRKRMMLVPGALLLLSILPAFYLLLHYRTAAAL
                     IGATAVLSSLATLSAGPTVIWLTESLPVAIRSSGVAVVYAISIATFGGTTQYVVTWLI
                     KMTGSSLAPAWYWTGAAIVGLIAMSVVRESAPRKLQNHKRVELIRSEAVSED"
     gene            complement(7670..8191)
                     /locus_tag="VG272_10260"
     CDS             complement(7670..8191)
                     /locus_tag="VG272_10260"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWF14107.1"
                     /translation="MGKLIQMSKVKISKEPGKSKIKRAEIEGFPGAIRMGIHGGIAQY
                     FKLSPEEPMASTLDYIVAAVGGCMTGTVAGALEARGVSANPDKLQVEAHGTIEDVDGK
                     MILTGIKMHYRLTVPKDKRATVERALEHHEGLCAASESVRRGITVEWDSEIIEESDAE
                     VGQASTATTAARS"
     gene            complement(8221..8796)
                     /locus_tag="VG272_10265"
     CDS             complement(8221..8796)
                     /locus_tag="VG272_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14108.1"
                     /translation="MQLQHVNVKLLLQTSQGISLDPLIPVFHSWIENQNGDELLIDVA
                     DYAHVPAGPGVVLIGHEGNYSVDNTDERLGVRYNRKAELNGDNQNVLAQAARAALTAC
                     QRLESEPRLNGKFRFNGQDIEFFINDRMIAPNNVATRDVIDFEFQLFCQKLFRGKEYA
                     ISYDMDPRTLLTAKVKAAQPFTVAQLLEALA"
     gene            complement(8945..11200)
                     /locus_tag="VG272_10270"
     CDS             complement(8945..11200)
                     /locus_tag="VG272_10270"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HWF14109.1"
                     /translation="MKVSNSKWTERLAGKLPEALAREIDNFETEIALRKQGKLDERLF
                     AETRLRRGVYGQRYDNGQRHNGKTVQRLGFPSGDLTKGPNTMWDAPGMQRIKIPAGGL
                     NAEQLETLADLAEEYSDGIAHVTTRQDFQLHYVHVEDTPTVMRRLAAANITTREACGN
                     SVRNVTACPYSGVCPDEKFDVTPYSRALSKFLLGHPDTQNFGRKFKHSFSGCSQHACG
                     LASMHDMGMVAATRIENGKEKRGFQVQVGGGLGAVPYQAKLYDSFVPVEELLPLAQAI
                     SLVFARLGEKKNRSRARIKFLVQDLGIEKFKELVADERKNLAPDPRWTDFVADAETFH
                     ETPLRPGGKAPLLGTESFQRWVKTNTRAQKQDGYTVVTITLPLGDITSKQLRSLSDIA
                     RRFTKETIRSTVEQNIVLRWVSLSDLPELHKALEAAGLGAPGAGSIVDIAACPGTDTC
                     KLGISSSRGLAAELRKRLAEKNFRLDDSVVKLHIKISGCFNSCGQHHVADLGFYGVSR
                     KMSGYAVPHFQVVLGGEWENNGASYGLPTIAIPSKRIPEVVSRLTDRYAADRKNGESF
                     KDFVGRTGKVELKRMLEDLARPPADLTDRSFFQDWGDPREYTLADIGVGECAGEVVSS
                     VEFDLAAAERQLFEAQVALEDGHIEQAGETAYRSMVQAAKALVKIESSGIADDPALIV
                     REFRARFYDTQKFFDPFAGGKFAHYLFTAQEKSGQPYTQDGTRYLIDEAQLFIDAAHS
                     CYNRISAPAPA"
     gene            11595..12260
                     /locus_tag="VG272_10275"
     CDS             11595..12260
                     /locus_tag="VG272_10275"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWF14110.1"
                     /translation="MATLYLTPVLVPALDNASKVEESGYNRARRFAPMTSDIDLQMLM
                     ARYQQGDLAAANSLIHCMSPRLHRFFAIQFTSREHADDLLQEMWLRIHKARHTYRPGE
                     PVLPWLYAIARNIRIDHFRKSSRTSGHEQSLDETPEVETAAAQEAERIPNLDVLLAPL
                     PESQREVITMLKVEGMSLEEVARATSSSIGSVKQKAHRGYEKLRERLTMLGLGKGQLG
                     EMP"
     gene            12257..12985
                     /locus_tag="VG272_10280"
     CDS             12257..12985
                     /locus_tag="VG272_10280"
                     /inference="COORDINATES: protein motif:HMM:NF018264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HWF14111.1"
                     /translation="MTESDVDEILKQAARAPQSVNPAVLERIAASMGSSLSPVSPLPP
                     TWALESGLIAVAIAIAMVGAAKAGFFGILKLNFVERMIVFPALGILIFLAASAWVGEN
                     IPGSKRRLTPGALFSITTLGILAVFGVLFRNYETQRFLSSGLICLRVGLLHAVPTAFL
                     GWLILRRGYAVNAVASGLVAGILAGLAGVTMLELHCPNFQALHVLIWHTAVIPVSAAS
                     GAFLAWTFAKRPTVSKQSSFAGFK"
     gene            13200..13697
                     /locus_tag="VG272_10285"
     CDS             13200..13697
                     /locus_tag="VG272_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14112.1"
                     /translation="MARKLLILLGLLFIGSISARAQSVGVLAGYSFEHVGTSPGRNFN
                     GIELGAQYRLLGWLSAVADLDGHYVFPNQSDGRSFHIMVGPEVSMPFGKYSPFVHALA
                     GYGSIHDNGSTSSSYAAALGGGLDRHIAPLFTWRMVQVDDVVTHYFGGLQHNVRVTTG
                     IVFRF"
     gene            complement(13738..14451)
                     /locus_tag="VG272_10290"
     CDS             complement(13738..14451)
                     /locus_tag="VG272_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14113.1"
                     /translation="MASRGTAPDGSKAERRKSHRFPVAVPIEASWRNSEGIPVLVQAV
                     AKQVNANGGYLEMSIHPSVGGRITLTNFLSAESAEARVLATPSSREGVSHGIIVELIT
                     PSDSFWGVNLQVKKTSVELQRLEKALQAEGIDFRLLNEFRDTVDSIRTTALAVQQLRE
                     LQLKGRDESEITALLISERIRRATNLCRELVADLDAGHVSNAVKGVDQLYESLEQACD
                     RLRHLLKSRHPDRYLTTRT"
     gene            complement(14668..15792)
                     /locus_tag="VG272_10295"
     CDS             complement(14668..15792)
                     /locus_tag="VG272_10295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWF14114.1"
                     /translation="MAKRYGFVVLIFLIFLPIGMVWLFAQDQNQNQDQNQNNSSDGSV
                     IHVVVSMVQLNVAVSDEKGNYITTLRPSDFTVAEDGISQKMASFEEGNAAPQTILNAE
                     QDSGQTIPPDKTAPNELQPNGVIRGGTQSGSLDAIASSVSGSNVFILFDTSNYMFRGR
                     GFVFAQDSIAEFVRSLDHPYQVAFYSYSRNFYRAASLTSDRSQVIRGVRETTNGDDSA
                     LYNALLMTLKDAAQHTGRKVVVVFSNGPDNSSMVSPEDVDELAQSEGIPIYIISTREA
                     QLNAASAAVFARMTASTGGEAYFAKSWKDQRDAFSSIREDLAHLYFMSYYPQPNPNRG
                     WRAISVKLNNPKFKKYRIRTRSGYRPIPARINTENLPPEQ"
     gene            complement(15874..18906)
                     /locus_tag="VG272_10300"
     CDS             complement(15874..18906)
                     /locus_tag="VG272_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14115.1"
                     /translation="MAAAPADPAQRSSAKTPHTVDEASIPGPMRSFLRMAAISQQITA
                     DELLPLLARNVVMNGYEVGRPTQYLVLVNWYLDQARELEALAGKEEVIHVSNCEDAKP
                     LLAILGYRLQKTCGPDAALETADANRAFLTVNSGFPLADLEDALRAGKSFDTAYGPTK
                     VPVLYKTSDWISTKKNSNRNVVDSILRDPNLARLYWAMSRMDSETSQFLWQSLGLKKL
                     VLVAPVLDFYGSEISVRSGRVLVPGGTQAESAWRDLVGASPRSPADFIFKLLTKDGGW
                     VASYYDTLSRVDSSQQVYFISSRRIQRLYDALRGHDINPLPTRHSFRPDPALFLLEAR
                     LLLNPNGEPHIPGSIDVWKEVLHRKVDSKLVREWGDKASSWNNPEQVIEGMVAVTRYP
                     LPDGPVFNFLMLTEIDRNRTASEQLTIPTARLLAEKFPLFGDQYPMFAEFPALTNESI
                     TRFLTVAEGLNQIPDHLARTDALGIMQADVGLWEILARQGEIPDAQLNDSWQKVLIPF
                     STLHSSDQVYQAGRASLAELLRAASGKTYLPQDQLFALLAGPEQYTKEGQQLRLQMAR
                     RMHQVIDDQRLVSLDTLISLGDGLDQMAKGKEVAESLLPLAGELREFELPRPLFTKSE
                     RNEFAGGLYSNRHTALQMRTDLIRIIKSPSSPAELVQAHALLTPFLRDTLVGLNYAYY
                     EPPGAQMIHHNPLFVRSHDFSGGDYSGEAATATDQIWRSPRIFGRGWTASGGAHLIGS
                     MADLPYALARVEQDFIVPKNVQSLIWEDLVPGLITSAVLPRWWSVSRNEMHAVALYQR
                     TGEELLSSAAKDAKVREQVVNIISERLLPQRLEQIEAALRTGREEDALALLAPSDTFY
                     LAAEFRRNHSSERQAWGPAGNELDEVLTRYPIETNLDRISQDFGVPHPAIAQTYHREL
                     MSMKPVPTFLYYSSRLMAESWDSGNLYWGRLADEMGYSPMMLNILVPELTRRMVEQTF
                     ASHLEDWSAVLRAMRETGEEFRQGKIASLAKKSPSAGL"
     gene            19485..20372
                     /locus_tag="VG272_10305"
     CDS             19485..20372
                     /locus_tag="VG272_10305"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWF14116.1"
                     /translation="MKIAFLGLGRMGAGMARNLIRAGHSLAVYNRSREKAEALAADGA
                     RVATSPADAARDADAVMTMVADDYALEQIIFGENGATSAMKAGCIHLSHSTISTDMAR
                     RLTSEHAQRKQGFLSVPVFGRPDAAESKNLIVMAAGPADLVERCRPLFDAIGRQTFVM
                     GTEPWQANVAKVCGNFMVISAIEALGEAYATLRKSGVAPETFLDVMHSLFGSQVITGY
                     GRIIAQEKFEPAGFALKLGLKDVRLVLAASEEVASPMPLASLLHDQLLAGLAQGQGEM
                     DWSSVTQVIARNAGLEAES"
     gene            20483..21343
                     /locus_tag="VG272_10310"
     CDS             20483..21343
                     /locus_tag="VG272_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWF14117.1"
                     /translation="MSLVDFIPTPKFEDYKERFKQHYKMERSKDGVIVVEAHTAGGPI
                     QLSVENHRSVGQLFKTIGADPENEVMIFTGSGEDFMMDADPEGFKLEQEDLQHWAYEY
                     AYKDGRINVSALVNDLEIPTIGVLNGPGFHTEICLMCDISICSEDAVIFDLHYDIGSV
                     PGDGIHSCFLELLGTKRGAYALLTGQAIDAKKALEYGLVNEVVPRDRLLDRAKQLAAH
                     IMTQPRITRRLTTQIIRRPWKKRITDDLDGGFGIQMFAHLAKKKSIHSRNHIATSVAY
                     VKEGKKNNIK"
     gene            complement(21412..22116)
                     /locus_tag="VG272_10315"
     CDS             complement(21412..22116)
                     /locus_tag="VG272_10315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWF14118.1"
                     /translation="MNLKNATALITGGSSGIGRAIAQSLVATGARVAITGRDERKLAD
                     VAKESGVLAIHADVSVEADVERSYKEVMQKFGDLDILVNNAGSGVFKNLVDMGKKEFE
                     DVFATNVTGAMLMAREAAKHFVKKQRGNIVNISSTAGLRGAPNGTAYYASKFALRGMT
                     ECWRAELRKYNVRVILVNPSEVVTNFYPTAGLPQKANASKLRAEDIAYVVKSMLEMDD
                     RGFAPEVSIFATNPQD"
     gene            complement(22229..22990)
                     /locus_tag="VG272_10320"
     CDS             complement(22229..22990)
                     /locus_tag="VG272_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14119.1"
                     /translation="MRNRLAIGILVLSVMASLAGAARGQGKPQQASAPPSAPNTISAS
                     AATDITGIWDGMRGNYDFASFSKGDPPMTPWGKAQFDAAKPSQGPRGVSLKETTDSVY
                     KCFPPGMPYIYLQIFPMRIVQTPKEVIEIFEYDHNVRHIYIDGRKHPDDLTPTYNGHS
                     IGHWEGDTLVVDTVGLNGKLWLDRLGHPESDQMHIVERIHRVDEKTLQVDFTFDDPKA
                     YPKPWNAVMKFRSRPDWDIMELICEDNIDFESFEK"
     gene            complement(23214..23903)
                     /locus_tag="VG272_10325"
     CDS             complement(23214..23903)
                     /locus_tag="VG272_10325"
                     /inference="COORDINATES: protein motif:HMM:NF027675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HWF14120.1"
                     /translation="MRRPPLITTKSSSSPGGNPSGSPRPSSIGPRYLHWKRRFAHLAR
                     WLHTYLSMVSFAILLFFAVTGLTLNHAEWFDSHARPAMYHGTLNKAWVNASGPDGVAK
                     DELVTYFRQTHRTKGALSDLHVDGEQCEILFKGPGYEADATIDRATGNYVLTISPFSL
                     VAVLNDLHKGRDTGKIWSDVIDFSAVLMTLVSLSGLTLIFFLNKRRFSGLLLVAVGAL
                     LCWLVYTFWVS"
     gene            complement(23863..25413)
                     /locus_tag="VG272_10330"
     CDS             complement(23863..25413)
                     /locus_tag="VG272_10330"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2271 domain-containing protein"
                     /protein_id="HWF14121.1"
                     /translation="MAKVKSQFTIMGIVALSLYMVGSLTAKARGKLYVFHYENVLGTS
                     LELKVSALSEAQSEKAEQAALAEIDREAHILSSWDPQSEFSNWFRTHGQPVHVSPELF
                     EVFSLFNTYRGMTGGALDPSAETITRVWKYAAAEKRIPSQSELDEAIASVRRVHWTLD
                     PLNQTATHTSDTPLALNSFVKSYIAGRAADAALNASGAQGLVINIGGDLIVRGDWREP
                     VDVSDPKSDAENGTPIAHLIVRDRAVATSGDYRRGVEIGGRHYSHIVDPRTGLPADQI
                     ISSTVIARSPAVAGALATAFSVLTPEQSARVAASVPGVEYLLVDKNGNRIVSAGWSAY
                     EAAPAPSMAMRTPAATFKTISVQSGAEQWDPKYVLTVSIELSLIEGYRIHRPYVAVWI
                     ENENHAPVRTVSLWFGKYKYLNELRAWSRDENTRSMSEDTHILNSVSSATRPPGKYSF
                     QWDGKDDAGNPVKVGKYNVMIEAVREHGSYQLLHQEMDFNGSPKQFQFPGGIEIASAT
                     IDYHKIIQ"
     gene            25776..26690
                     /locus_tag="VG272_10335"
     CDS             25776..26690
                     /locus_tag="VG272_10335"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HWF14122.1"
                     /translation="MKRAMMRLFFAATVAAFLFSGQANLRAQATPSAQHKAKAPRSVR
                     LYVFDCGVLHNADMGRFNLKNEDVTTSDMSMACFLIAHPKGNLMWDTGAIPDDSWKPT
                     GAMITQHVSLPDGSSRDADVDKNLRDQLKEIGYSPADIDYLALSHYHYDHTGNANEFA
                     SATWLVPQVEHDVMFGGAKVYGSLWPATFSALKNSKTIILKENEYDVFGDGTVIIKSA
                     PGHTQGHQVLYVKLAKTGGVVLSGDLYHYPAEKTLDRTPTFDFNPELTRASRKEVDAF
                     LKKTNSQLWIQHDFAGNAKLKKSPQFYE"
     gene            complement(26921..27886)
                     /locus_tag="VG272_10340"
     CDS             complement(26921..27886)
                     /locus_tag="VG272_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14123.1"
                     /translation="MKSRTFWIFAPGVSLRRRVAYSLAVVRLILAPVILLAIYYLFQM
                     GWIVDRIVSVDAPSATLAQQASIQMLEARRAERNFFLLNDPADVKANHDSLAAASGIL
                     RQIRTLHPDEEATIQIALNSVNLYQQRFDEAVSYLSQPGEAQRDRLETVLRNYESDLN
                     TLVRQDNRKSRAKLVEQLRAQVDSFDTQIMSTIQASDPKLRQIAGALDVNSRELFGLT
                     SALEGENWERVQGDHERAQKLLHRAEWVLSIVSAITIFLSLWVSLVLPRQVIRPLMIL
                     KEAVDHAISGNYQIQFELQGKGEVVELAKSIRSLIAHLHARRQTA"
     gene            28209..29594
                     /locus_tag="VG272_10345"
     CDS             28209..29594
                     /locus_tag="VG272_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14124.1"
                     /translation="MKLARRKIVVGAVVGAMIGATILLSWVNTKRVSAAQTGQWVDPA
                     NPSDELVDGPYRVDPNWPKPLSTLFPEEKGWTWGAVQGVFAQNPDRIFVVMRGELPDL
                     ANVKKAYVELETAFGLKAHVEVPGRGMYGRNASVGPYASPGEASDDYLGVDGKDYRWK
                     HIITVYDSKGDVKEAWTQWDSLFKPDNPNVLSQGRVHKILISPYDPEKRVWAIDDGHE
                     VIRIFSNDGKKLLQTIGTLNTTTERNAKMTGNTARPSYIQKTGPPSETFGRQTDIAWL
                     PDGTFFVTDGYEETRVVKFDKNGKFLMAWGERGAAGGHEMRPNYFNTVHGIAIDNQRR
                     LYINDRSNRRIQIFDENGKFLNQWYLGDGPTATYHIFMTADQHLWMSDGHGNFKFYKY
                     DLNGKLLYTWGTMTPEAGGLWGTHQFGVDQEGNLYTAEVWGGRPQKFVPRKGADPALL
                     IGQPIRAAWTK"
     gene            29857..31125
                     /locus_tag="VG272_10350"
     CDS             29857..31125
                     /locus_tag="VG272_10350"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14125.1"
                     /translation="MTKRKFTLLISAVASIAFVAGVIATSKMISVQAQTKPGYGFAAL
                     AGERVGQDVDGPYQVDPNWPKPITALPGHEKWTYGAVESVFAESPNRVFILERGEIPA
                     MKRPEEIPYPSVAPSISFPVAQVPWRNASVGPVTSAGNPVWKGTLGVDARWEHCIVVV
                     DANGNLIEDWTKWDKMLRRPHSVTMNPYDPEKHIWVVDDQGHAVYEFTHDGKELVKTL
                     GTPYQPGEDDKHFNRPTFINWLPDGTMFVTDGYGNTRVVKFDKDGKYLTAWGQPSNPP
                     DDTRPGTFHDVHGVVFDVPRHRVFVTDRSDHRIQIFDENGKFLDQWTTGKPSTPQFLY
                     KAADGFLWIADGYTAKIIKFDMDGHLLYEWGSEGDWPGALWNVHGMSVDQEGNLYLAE
                     VNNGRAEKFKPRPGANPALMVGKPLYSAWK"
     gene            31263..32228
                     /locus_tag="VG272_10355"
     CDS             31263..32228
                     /locus_tag="VG272_10355"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF14126.1"
                     /translation="MRIITLEEHITTPEMLKAAQMPATGASAAFMQTMNAKLLDVGEG
                     RIADMVAAGIDMQVLSVSANTVDKLDSAAANAVVRDANDRMAAAVRAHPDRFAAFATL
                     ALHEPDKAAAELDRCVRKLRFKGVMLNGASNGQFFDHPRFTSIFEAATSLDVPIYLHP
                     APPPKPVMEAYYSGLPGSLGFMLSTAMWGWHVETGMHCLRLILGGLFDRFPQLKIIVG
                     HMGENLPFSIARAEMVLGRGAADLKRSVGEYFQEHFYLTTSGYFSTPPFQCMQQVVGI
                     DRIMFSVDYPFSSNTVGRKFLDSLPISPEEKERIAHGNAEKLLKI"
     gene            32336..33022
                     /locus_tag="VG272_10360"
     CDS             32336..33022
                     /locus_tag="VG272_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14127.1"
                     /translation="MKKIKPVIAAMALSLVGAYITLAQQPAQQAPSQPMSFFITSAGS
                     GHGANLGGLAGADRICQTLATAVGSGNKTWHAYMSTNAANGQPGVNARDRIGNGPWYN
                     AKGEKIANNLADLHGDTAELARLGNNLVKMSALTEKGAMLNGRGDTPNVHDMLTGSQT
                     DGRAYTDGMDHTCSNWTSETTGTAQLGHFDRNGANNTSWNSAHPSRGCSQDNLVSTGG
                     AGLFYCFAIN"
     gene            complement(33196..>33969)
                     /locus_tag="VG272_10365"
     CDS             complement(33196..>33969)
                     /locus_tag="VG272_10365"
                     /inference="COORDINATES: protein motif:HMM:NF016292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWF14128.1"
                     /translation="EILSAHAQGEISNIPLLIENYDSQRLNAIITMTTPCLAAACNLA
                     KKTPVVFTYCYDPIAAGAGKSMTDHLPNITGVGSFPPVDDTVALIQKLVPNVKAVGTV
                     YNSSEANSVKVISVARESFKKRGIKLEEVTATSTSEVFQAAQVVSYRGVQALWVTGDN
                     TALQAFDGIVKASRDAKLPLIINDPEFTMRGAVASVGLGWYEAGHAGGVLAARVLRGE
                     NPKDMPFEEVVVKKIVLNDDAAHRLGLVFPPEIVKAAKG"
BASE COUNT         8000 a         9160 c         9100 g         7709 t
ORIGIN      
        1 tggtcgattg gactgccgcg cgacaggctc acgcccgcgt ctatgactct ttgctgaagg
       61 atttgcccgg cgttctgatt ccgcgtgttc cgcttgggag agagcatgtc tattatctct
      121 acacgatccg agtggaaggg cgcgccggag gcaataccag gtccaacgat cgcgggaatg
      181 aagtgcgcag cgatcttgtg cagcagcgct taaagggtcg cggtattggc agcaccgtgt
      241 actaccctgt tccgctccat ttgcagccgc tctacgcgtc gcttggcggt aaaccagggg
      301 aattgcgcgt cagcgaacgc gcctcccgcg aagtgctttc gattccgatt tatcctgaac
      361 ttacacccga acaggtcgag cgagtcgcgc aggaagtgcg caaagccact caagaagatt
      421 agatttcaga tttgaaattt tagatttcag ttttttagca ggacagactt cagtcggtct
      481 attcttgact tctgccaccg ctcaaaaaat tcaaaagaag acaggctgaa gcctgtccta
      541 ctgaaatctg cgtcacattt aaaatatttg gtgtgttaac gggggcttat gcggttgcgg
      601 aaactgcgcg gcggtagagc ataaatatga cgctgagaaa caggatcaag gccggcgaca
      661 ccaaggcgag aattccgaga tcgagcgagc gttgcgcgga aacgcccatg gcggatgcag
      721 tcgtgtagca cagtgcgcaa ccttgcgcga acgcgccttg cgccgaaact gtcatcgcca
      781 acaatgctgc caaagcaaat tttatacgca tttcagatcc tccaaaaagc attccggccg
      841 cgaacccatc ttacctcaat gtggcctcat ttgaagcagc cggaaggcct ccgggaagaa
      901 aaagataaac atcatgcaga tacaaaaaat catggccggg accagccaga gaaccagcga
      961 taacttttca aatttcaaat gcatgaaata cgacatgatc atggccgcct tcaccacgga
     1021 caagacgacc aggatgctga gcattatcga cggctgcagt tgcttgtacg caagaaaaac
     1081 ttcgaggccg gtgatggcca cgaggccaac ccagatcgcc aaattggttc caacactggc
     1141 accggcgtgt atttcttcgg catgggcatg tgtgttcatg gatcgactat tctccccaat
     1201 cgcaggctta gtacttcacg gacattaaat agatcagcgg gaaaacgaac atccatacaa
     1261 ggtccacaaa gtgccagtac agcccgttca cttccacatc ctcagcggaa aatttcccgc
     1321 gcccgtaacc cacggccacg attcccaggt agatcacgcc gatcatgacg tgcgtcatgt
     1381 gcaggcccgt aataccaaag aatacggcgc cgaaaagcgg tgtgccttcc ggatttccgg
     1441 tgattgtgaa atgctctttg gtgatcatgt tgaaccactc ggttaagtgg agaccggtaa
     1501 agagcagccc gccgatcata gtggcaacca tccacatgac tgttttcttg cgatcgttgc
     1561 gctgcgcggc cattacggcc atcaccatgg tcagcgagct tgccagcagg accacggtca
     1621 tggtcgtcga gaatgcaatc gcgggataaa tgggaaatgg gcggggccaa tcaggattcg
     1681 tgatgcgcga atagctgtac gcgatgagca gcgcggaaaa agtcagcgta tcggaaacga
     1741 tgaaaagcca catccccaat ttttttgaac ctattgcaaa aggagatttc ccaccattcc
     1801 atgctgaatc cagcgccacg tcattcttaa cctcaagcac ggttcaccgt cccagtgtca
     1861 gaagtgccag caagaaaatc caaagcccat ccatgaaatg ccaataaatt gaagtcaact
     1921 cggcagccgt agcctgcgaa atacgcgatt ttctccaggg ccggaacgcc acgtagaaca
     1981 gagcaaaaat tccgcctatc agatgcgcgc cgtgcgctgc cgtcagcaca tagaaaaagc
     2041 tgctgctagg attcgtcgcg agaagcattc ctgcggcggc gagttggcgc caggcaatca
     2101 cttgccccgc caggaaaagt actcccaacc ctgtggtcac ccaccatagg gattgaaatg
     2161 cctcacgctc gccgctcgcc aacttttttc tcgccaccac aatggtgacg ctacttatga
     2221 gcaggaccgc tgtattaaac caaagcaccg gaggaatcgg cgtgcgttgc caatccgtcc
     2281 caaggcctct tcgcacaatg taagaactcg ccatggccat aaaaaacatg acgatggccg
     2341 ccaagccaag ctgcaatgcc gtgaagtacg cgcggcgcgg aaatggccgt cgcgaaccgt
     2401 gcgaatctcc atcaccgtcg ccgccagccg gcggaggagt tccgccaccg tgatctgcat
     2461 ctataatctc aacatcatcg aaaactgttc ctggcattgt ttaatcctga agagcctatt
     2521 ccgtggagcc ctcgctcttg cgaattaatt ccggcgcaag gtgctgggga atgtaatcat
     2581 ccgctgcgcc cggtacgctg aactcatatg ctccatgata cacaacgggt tctactccgc
     2641 caaaattatc aaagggcgga ggcgagggga tcgaccattc cagagttgtc gcatgccacg
     2701 gattttcttc cgtggctttc tcgccagcaa gaaggctcca gaaaaaattg aacaagaaaa
     2761 tcaactgggc accaacggtg ataaatgccg caacgctgat gaaccggtga agaggctgca
     2821 gctgctgcag gaacggaccc gcagtcattt ccgaatatcg ccgcggattc ccagctaatc
     2881 ccagaaagtg catgggcatg aacacggtgt atacgccgat aaacgtgaac cagaaatgaa
     2941 ccttgccgac tccctcgttc ataaaccgcc caaacatttt agggaaccag aagtacagcc
     3001 cggcaaacat tccaaagatg gccgcgacgc ccataatcag gtggaagtgc gcgacgacga
     3061 agtagccgtc atgcagatac aaatcgagcg caggctgcgc gaggaacaaa ccggaaaggc
     3121 cgccggtcac gaatagggaa acgaagccga gagcaaagag catggcgcag ttaaaggtga
     3181 tcttgccgcc ccacaaggtg cccagccagt taaaagtttt gatcgcagag ggcacgccaa
     3241 tcgtcatcgt aagaatcgaa aacgcgaatg ccgaatacgg gctcatcccg ctgatgaaca
     3301 tgtgatggcc ccagacgcag aagccgagga agccgatgcc gcagaccgcc ccgaccatcg
     3361 ctcgataacc aaatacaggt ttgcgcgaga atgtggagag caggtgggac gcgacgccca
     3421 tgcccggaag aatcgcgatg taaacctcag gatgtccgaa gaaccagaac agatgctgcc
     3481 acagcagcgg cgagccgccc ttgtgccacg tgatgacttg gtccgtgacc acgagaccgc
     3541 cggggacaaa gaaactagtt cctgcaacac gatcgagcaa tagaaggatg catgcagcga
     3601 gtagcacgcc gaacgccagc agaccgagga tggccgtgac gaaccatgtc cagcaggtca
     3661 gcggcaaccg tccgagcgtc atgccctttg cgcgaaggtc aatcagcgtc gtaataaaat
     3721 tcagcgcgcc catcagcgat gcgccgcaga aaattgccaa gccaataatc caaagtgtct
     3781 cgcccgcacc ctgccctggg ccaacaattt ctccaagcgc actgagcggc gggtacgagg
     3841 tccagccacc tataggcgcg ccgcccgaga ccaaaaacgc gcacatcata acgacgagcg
     3901 atacgaatgt cgtccagaac gaaagcatgt ttaggacggg aaacgccatg tctgccgcgc
     3961 caatttgaat gggcaggaaa taattgccgt agccggattg cggcgccgtg gtgagcacca
     4021 tgaagaccat gatcgtgccg tgcatggtca tcagcgccag gtactgttcc ggcgtcatct
     4081 ggccgccctt gaagaaagca aattttgcgg cgggccatcc gagatgcagg cgcatcaata
     4141 aggaaaggcc catgccgacg aaagccgaga acaaggccag gcacaaatat tgaatgccga
     4201 ttactttatg atcgatgctg aacacatatt tccgaatgaa accggtcggt gcgtggtgcg
     4261 cgtggccgcc gttttcgtgt gtcaccattg aggctctcct cagggtcttt ggtagaacag
     4321 gcttcagcct gttgattttc cttaccgcaa agcaaatgcg aaatcggcag gctgaagccc
     4381 gtcctactga aaatttactg gttcgctgct tcctgttcct tcgcccactt atcaaaatcc
     4441 gccgggggca tcacagtgag catggagtgc atgcggtagt gacccaaccc gcaaagctct
     4501 gcgcaggcaa tctcatagtc gcccggttcg gtggcattga agtgcatgtg aatttccatg
     4561 ccgggcacgg cgtcttgctt gagccgcaat tcgcgcacgt agaaactgtg agtgacgtcc
     4621 tgcgatcgga tgatgagctc gacttctttg ccggccggaa cgacgatgac aggagaaaca
     4681 atgtcatcct ttgaggcagg gtcggtagga tcgaggccta cgggatttcc tgtagaggca
     4741 ctaaccagtt caggcttctc gcgcccgaac tttccatctg gcccagcgta acgaaaattc
     4801 cagacaaatt gttggccggt cacttcgatt tgcaatgcgc caggttgggc gcccatgaaa
     4861 tgaacttccg cccaggcgtt ccgcgcatac aggcccaggc cgacgaagag caccacggtt
     4921 gccagcgtcc agacaatctc catggtgttg ttgccttcga aatacgtcgc cttctgtcca
     4981 tggtcgcgaa atttgaaaat ggcgatggca aggccgagtt gggccgcgac aaatacaatt
     5041 cctgtgatga ggaacgtacg cgcaaattgc gagtcaattt catggccaaa atcggtgatg
     5101 gcgggaggaa accaccagat ttttgccatg aagatgtata tggtcacacc tgtcatcaaa
     5161 aacagaaaca cagcaaaggc aagagcaact gaggtggtac cgcgttctgg ccgtctactc
     5221 agcatcgtct tcgctccaag ggtctttaaa gacctaacaa cggggcccga agtcattttc
     5281 caagacattt gcgcatgacg aatccaatcg atatgtatgg cctaatgcgc acatgatata
     5341 caacctgaga agaaattcaa caagctttat tcgctctctg aaaataattt ttaaagacga
     5401 aggaatgctt tttcaggatg ccgatctaat cgatcgaatt ctgagaaagc gaggtagaat
     5461 ataaaacatg cgaaaacaca gcgcatttct cgttattgcc cttgctgcgc tggccacatg
     5521 cgccattccg gtcgcggcgc aagttctcag ccctcccgcc aaggctgcct gtaaatttgc
     5581 tgacggcaaa atgatttcag tgaattattc gagtccgcgc atgcgcggcc gcaaaatctt
     5641 tggagatctc gttccctttg gagaagtctg gcgagtagga gccgacgatg cgacgtcgtt
     5701 tgtgccgaac gcggatgtca cagttggcgg caaaaacatt ccggcgggca ggtacaccat
     5761 attcgcgctg cccacgccaa acaggtggac tttgatcgtc agcaagcaga tcggcgaatg
     5821 ggggattccg tatcccggcg cacaattcga tttcgcgcgc atggagatga aagtttccaa
     5881 actttcggcg ccacttgaaa attttacaat ttctttcgac gcgccgggaa gtgcctgcac
     5941 gatgaagttc gattgggaaa caacgcgcgc ttcgatcgat atctccgaaa aaaagtagcg
     6001 gccagcaaaa agctgcgatt ggccgtaggg gcacggcaat gccgtgcccc tacaatttgg
     6061 cgaggatgcg acatccctca gcgaaccatt tgccgatttt taagcggaca ttcgagaaat
     6121 atggattgtg ccgcgaggaa taatttagtt tccgggaaag aatacccggt atataagtgc
     6181 gcgcgctaga gagatcgcaa gatgacaaca gctggctctc gtcaaactat tcctggaaaa
     6241 actgcatccg ttcccggtcg ccacatcgcg gctgttgtcg tgggcaacgc tctcgaattc
     6301 tacgactttt tgacctacgc atttttcgcc gtatacatcg gcaggacatt tttcccatcc
     6361 agcattcctt ccgcgagttt gctcgcatct ctcggcacat tcggcgtggg attcgtcact
     6421 cggcccatcg gcgcgtgggt gattggcagg atgggcgacc gcatgggacg aaagccggcg
     6481 atggtttttt ccatcgcgtt gacgggcgtc gcgatcacgg gccttgcctt aacgccatcg
     6541 cacgcgagaa tcggcgtggt tgctcccata ctcgttatat tttttcgctt gctgcagggc
     6601 tttgctcttg gcggcgaagt gggaccgacc acggcgttcc tgttggaagc tgcgccgccc
     6661 gagagacgtg gtttctatgc ctcgttccag ttgtggtcgc aacatttgtc ggtactcgcc
     6721 tcaggccttg ttggatttgg tttggcgaat ttgctcagcg agcagcaatt gcaggattat
     6781 ggatggcgca tcgcttttct ggttggcgtg gcgatagtgc cgctgggcct cgtgatgcgg
     6841 cgcagcttgc cggaaacatt tcatgcgccg tcttccggca gtgcgagcga gcccgcatcg
     6901 ttgcgtccac acataggaat tgcggtgctg ggtgtaatgc tgcttgcgag cacgacgatc
     6961 ggaaactaca tcgacaacta catgacgacc tatgcgatca caaccttgca tatgcgcgtc
     7021 aacattgctt tcgcggcgac cattattgtc ggcctttcgg gcgttacgtt cgatctgatt
     7081 agcggggcgc tatcggaccg catcgggcgc aaacggatga tgctcgtgcc cggagccctt
     7141 ttgctgcttt cgattcttcc tgcgttttat ctgctcttgc actaccgcac ggctgctgca
     7201 ctaatcggcg ccacggctgt gctgtctagt ttggctacgc tttccgcggg gccaacggtt
     7261 atttggctga cggagtcgct gcccgtggcg atacgctcca gcggcgtcgc cgtcgtttac
     7321 gcaatttcca tcgcaacatt tggcgggact acgcaatacg ttgttacatg gctgattaaa
     7381 atgacgggga gttccttggc acccgcttgg tattggacgg gggcagcgat tgtaggactc
     7441 atcgcgatga gcgtcgtccg tgaatcggct ccgcgcaaat tgcaaaatca caagcgagtt
     7501 gaattaattc ggagtgaagc ggtaagtgaa gattgaagaa ttgttgtggc gccggcgtct
     7561 tgccagcggg tttccattcc tatctcacta tcactcagaa aaaaccgccg gcaagatgcc
     7621 ggcgccacgt gttccggacg gcgcggatgc aaaatctggg ccgcccgctt taagatcgtg
     7681 cagccgtggt tgctgtcgat gcctggccca cttccgcatc ggattcttcg ataatctcac
     7741 tgtcccactc gactgtaatg cctcggcgca cgctttccga tgcagcgcaa agcccttcgt
     7801 gatgctccag cgcgcgctca accgtggcgc gtttgtcttt cggcacggtc aaacgataat
     7861 gcatttttat gccggtcagg atcatcttcc catctacgtc ttcaatggtg ccgtgcgctt
     7921 cgacctgaag cttgtccgga ttggcgctca cgccgcgcgc ttcgagagct ccggcaacgg
     7981 ttcccgtcat gcagccgccg acggcggcaa cgatgtaatc gagtgtggat gccatgggct
     8041 cctccggcga aagcttgaag tattgcgcga tgccgccgtg gattcccatg cggatcgcgc
     8101 cgggaaaacc ttcaatttcg gcccgtttaa ttttgctttt gccgggttcc ttggaaattt
     8161 ttaccttcga catctgaatt agtttgccca ttagaaatgc tcctccataa agaaataaat
     8221 ttatgccagc gcttccagca attgcgcgac tgtaaatggc tgagccgctt tcaccttcgc
     8281 cgtcaagaga gtgcgcggat ccatatcgta agaaatagca tactccttgc ctcgaaatag
     8341 tttctggcaa aagagttgga attcaaaatc gatgacgtcg cgggttgcca cattgtttgg
     8401 tgcaatcatg cggtcattga taaaaaattc gatatcctgg ccattgaaac ggaattttcc
     8461 gttgagccgg ggttccgatt ccaatcgctg gcacgccgta agcgccgcgc gcgcggcttg
     8521 agcgagaaca ttttgattgt ccccgttcaa ctcggctttg cgattgtacc gcacgccgag
     8581 gcgttcgtcc gtgttgtcga cgctgtaatt cccttcgtgc ccgatcaaca caactcccgg
     8641 cccggcggga acgtgcgcat aatccgcgac atcgatcagc aactcgtcac cgttttgatt
     8701 ttcaatccag ctgtgaaata ccggaatcag cgggtcgagg ctgatgccct gggaagtttg
     8761 gagcagtagt ttcacgttga cgtgctgcag ttgcattctt gggttccttt tttctatgta
     8821 gcgcccgcct tcaggcgggc atgtgttgga tcgccggacg cttttctaaa cgatggttct
     8881 ctaaacccgg atgccaacct gaaggttggc gctatatccg gtacatcaag cctgcgactt
     8941 tcatttaagc tggtgctggg gccgaaattc ggttgtagca gctgtgcgcc gcgtcgataa
     9001 agagctgagc ttcatcgatc aggtagcgtg ttccatcttg cgtgtagggc tggcctgatt
     9061 tttcctgcgc agtaaaaagg tagtgagcga atttaccgcc cgcgaatgga tcgaaaaatt
     9121 tctgcgtgtc atagaaacgc gcgcggaatt ctcgcacgat cagcgcagga tcatccgcaa
     9181 tgcccgaaga ttcaatcttc accagggcct tcgcggcctg gaccatggac cggtatgccg
     9241 tctctcccgc ttgctcgatg tggccgtctt cgagcgcgac ttgcgcctcg aacaattgcc
     9301 tttcggcggc agccagatcg aactcaacgg atgaaacaac ttctcccgcg cattctccta
     9361 cgccaatatc ggctagcgtg tattcacgcg ggtcgcccca atcctgaaag aatgaacgat
     9421 ccgtcaaatc agcggggggc cgcgcaaggt cttcgagcat tcttttcaat tctacttttc
     9481 cggttcgtcc gacgaaatcc ttgaaacttt cgccgttctt gcgatcggcc gcgtagcggt
     9541 ccgtgaggcg cgatacgact tccggaatcc tcttcgacgg aattgcgatg gttggcaagc
     9601 cgtaggaagc accgttattt tcccactcgc cgcccagaac aacctggaaa tgaggcacag
     9661 cgtatcctga cattttacgg ctgacgccgt aaaagcccag atccgcaaca tgatgctggc
     9721 cgcaactgtt gaagcagccg ctaattttga tgtgcaactt tacgacggaa tcatccaacc
     9781 ggaaattttt ctcagcgagg cgcttgcgca attccgcagc gaggccgcgc gaagaagaaa
     9841 tgcccagctt gcacgtgtcc gttccggggc acgcagcaat gtccacgatg ctccccgctc
     9901 cgggagcgcc caaaccggcc gcttcaagag ctttgtgcaa ctctggcaaa tcgctcaggc
     9961 tcacccaacg aaggacaatg ttttgctcga ccgtcgagcg aatcgtttct ttcgtaaacc
    10021 ggcgcgcgat gtccgacaaa gaccgcaact gtttggaagt aatatctcca agcgggagcg
    10081 tgatggtcac aaccgtgtag ccatcctgct tctgcgcgcg tgtgtttgtt tttacccagc
    10141 gctggaaaga ttccgtgcca agtaatggcg ctttcccgcc aggccgcagc ggcgtctcat
    10201 ggaatgtttc ggcatcagcg acaaaatccg tccatcgggg atcgggagcc agattctttc
    10261 gttcgtcggc aaccaattcc ttgaattttt cgatgcccaa atcctgcacc aggaatttta
    10321 tgcgcgcgcg gctgcgattt tttttctcac ccaggcgggc gaataccagg gaaatcgctt
    10381 gcgcgagtgg aagcaattct tccaccggaa caaacgagtc gtagagtttc gcctggtacg
    10441 gaaccgctcc gaggccgccg cccacttgca cttgaaatcc acgcttctct ttcccgttct
    10501 caatcctggt cgcggcaacc atgcccatat cgtgcatgct ggcgaggccg caggcgtgct
    10561 gcgagcaccc gctgaaagag tgcttaaatt ttctgccgaa attttgcgtg tcgggatgcc
    10621 ctaataaaaa cttggaaagc gcacgggaat acggcgtgac atcaaatttt tcgtccgggc
    10681 aaacgccgga gtaagggcac gccgtaacgt tgcgaaccga gtttccacag gcctcgcgcg
    10741 tggtgatatt cgcggcagcc agacggcgca tcacggtggg tgtgtcctcg acgtgcacat
    10801 agtgaagctg aaaatcctgc cgagtggtta cgtgtgcgat gccgtcggaa tattcttcgg
    10861 caagatcggc cagagtttcc agctgctccg cgttcagccc gcccgccgga atcttgatgc
    10921 gttgcatccc cggcgcatcc cacattgtgt tcgggccctt ggttaaatct ccggagggaa
    10981 acccgagtcg ctgcacggtt ttgccgttgt ggcgctgccc attgtcatac cgctggccgt
    11041 acactccgcg gcgaagccgc gtttcggcaa acaagcgctc gtcaagtttg ccctgcttgc
    11101 gaagtgcaat ttccgtttca aaattatcga tttcacgcgc cagggcttcc ggtaattttc
    11161 cggcaagccg ttccgtccac ttagagtttg aaactttcat ttggcccacg cctccatcac
    11221 aatcgatcca ccaaaaaagc ccgccgggac aattccaccg cttgcatgcc gggcagcttc
    11281 actagaattc gcaattcgat gcggacctac tccgaatagc acccttaaaa aacaaaaacc
    11341 cacgtcggac gtttcgccga ggtgggctac ttgcgctgcg atcgttggtt ctttatcgcg
    11401 cgattccccc acacagcggc gcactgttgc agcaacaaca aaagcatacg ccgatcatga
    11461 tcctatggtt cacgggacta aaactatcat acagcggcgc actggaaagt caattttgag
    11521 ggcggcatat tgcgctgcgg aaaaggtcgc gcgtaacttt cattcccagc ccttcgtaac
    11581 cttcaatatg ggatatggct acactctatt taacacccgt tctggtaccc gctctagata
    11641 acgcgagcaa ggtcgaagaa tctggatata atcgggcgcg acgattcgca cccatgacct
    11701 cggacatcga tctacagatg ctgatggccc gctatcagca aggcgatttg gcggcggcca
    11761 attccttaat tcactgcatg agcccgcggc tgcaccggtt ttttgcaatt caatttacca
    11821 gccgcgaaca tgccgacgat ttattacagg aaatgtggct gcgcattcac aaagcgcggc
    11881 acacctatcg ccccggggag cccgttcttc catggctata cgcgattgcc cgcaatattc
    11941 gcatcgatca cttccgaaaa tcgagccgca catccggcca cgagcagtcg ctggatgaaa
    12001 cgccggaagt cgagacggca gctgcgcagg aagcggagcg aattcctaat ctggatgtgc
    12061 tgctggcgcc gcttccagaa agccagcgcg aagtgatcac gatgctgaag gtggaaggga
    12121 tgtcattgga agaggtagcg cgcgccactt cgtcgagcat cggttccgta aagcaaaaag
    12181 ctcaccgcgg ctacgaaaaa ttgagagagc gattgacgat gctggggctg ggaaaagggc
    12241 aattaggaga aatgccatga cggaaagtga cgtggacgaa atcctgaagc aggccgcccg
    12301 tgctccgcaa tcggttaacc ccgccgtgct cgaacggatc gctgcgtcga tgggcagctc
    12361 gttgtctccg gtaagtcctt tgccgccaac gtgggcgctt gaaagcggct taattgcggt
    12421 tgcaatagcc atcgccatgg tcggcgcggc aaaagcggga ttttttggca ttctcaaact
    12481 taattttgtt gagcgcatga tcgtttttcc agcgctaggc attctgattt ttctggcggc
    12541 ttccgcgtgg gtcggcgaaa acattcccgg aagcaagcgt aggttgacgc caggagcgtt
    12601 attcagtatc acgactcttg gcatccttgc ggtgtttggc gttctatttc gcaactacga
    12661 aacacagcgt ttcctttcct cgggactgat ttgccttcgg gtcgggctac tgcacgcagt
    12721 gcccactgct tttctaggct ggctgatctt gcgccgcggg tatgcagtga atgccgttgc
    12781 atcgggactt gtcgctggaa ttctcgcggg ccttgctggc gtaacgatgc tggagttgca
    12841 ttgccctaat tttcaagccc tgcacgttct gatctggcac accgccgtaa tcccagttag
    12901 cgcggcgtca ggggcatttt tggcatggac gttcgccaaa cgccccactg tctcgaagca
    12961 atcctctttc gcaggtttta agtagctgtt aagccgtcta ccattctgtt tttgcagtga
    13021 atcgcctcaa aactattctc taaccatatg tttatcaata agttagaaac ataagaaccc
    13081 tgagtaaccg gccggagact cgcagcatct gactaaccac ccagcaaggc gtatgtattc
    13141 cgacgcaatc ctcgtgcgcc gtcaatctca tcgaaatcca cgacttcgga ggccgtttca
    13201 tggctcgaaa gctactcatc ctgttaggac tattgttcat tgggtcgatc tctgcgcggg
    13261 cacaatctgt tggagtcttg gccggctatt cgttcgaaca tgtgggtact tcccctggac
    13321 gaaatttcaa tggtatagaa ctgggagcgc agtacaggct tttgggttgg ctcagcgctg
    13381 tggcggacct ggatggccat tatgttttcc cgaatcagtc ggatggaaga tcgtttcata
    13441 ttatggtcgg acctgaggtt tccatgccgt ttgggaaata ttctccattt gtgcatgcct
    13501 tggcaggcta cgggagcatt cacgacaatg gatctaccag ttcgtcttat gcggcggcgc
    13561 tcggcggagg tctggacagg cacattgcgc cgttgtttac atggcgcatg gttcaggtgg
    13621 atgacgtggt aacgcactac ttcggcggtt tacaacacaa cgtacgagta accacaggaa
    13681 tcgtgtttcg gttttaggcg ttactttaat tgagtagcaa ttgcggcaac gaggagatta
    13741 ggttcgcgtg gttaaatagc gatccggatg gcggctcttc agcagatgcc gtaagcggtc
    13801 acaggcttgt tcaagagatt cgtaaagctg atctacaccc ttaacggcgt tggacacgtg
    13861 gccagcgtcc agatcagcaa ccagttcgcg gcaaaggttt gtggcgcggc gaatgcgctc
    13921 tgagatgagc aaagccgtaa tttcgctttc gtcccggccc ttcagttgta gttccctcag
    13981 ttgctgaacc gcaagcgctg tagtgcgaat ggaatcgacc gtatcgcgga attcattcag
    14041 caaacggaaa tcgatgcctt cggcttgaag cgctttttcg agtctttgga gttcgacgct
    14101 cgtcttctta acctggagat tcacgcccca gaaactatcg ctcggcgtga tcagctcgac
    14161 aataattccg tgagaaacgc cttctcgtga gctaggcgtg gcaagtacac gcgcctccgc
    14221 tgattccgcg gaaagaaaat ttgtaagagt aattcggcca cctacgctgg gatgaatgga
    14281 catttccagg tagccgccat tcgcattcac ttgcttcgcg acggcctgaa ccagaacagg
    14341 tatgccttct gaattgcgcc aacttgcttc aatcggcacc gcaaccggaa agcggtggct
    14401 tttccttctt tccgccttgg atccatctgg ggcggttcca cgacttgcca agatcgccga
    14461 tttccttctc tccatgtaga attctcttcc ctgcgcggat gacggccggc acccggtgca
    14521 attcacgaat cacagatagc ttaaggacat gccctgaaaa ttactactgg acgaaagtac
    14581 agtaggcaac acaagcaact ctcagggcaa tcatgtattg cataaccggg ggaggttcag
    14641 gcggtaactt ggaaaacgcg agaaaattta ttgttcagga ggcaagtttt ccgtgttaat
    14701 gcgagcggga atcggacggt agccgcttcg ggtgcggatg cgatattttt taaattttgg
    14761 attattgagt ttcacggaaa tggcgcgcca gccgcggttt gggttgggtt gtggataata
    14821 actcatgaag tatagatggg ccagatcctc gcgaatggag ctgaaggcat cgcgctgatc
    14881 tttccaactc ttcgcaaagt atgcttcccc gcccgtactg gccgtcatgc gcgcgaaaac
    14941 ggcggcggaa gcggcattca gttgcgcttc ccgggtgctg atgatgtaga tcgggatacc
    15001 ttcggactgc gccagctcat ccacatcttc gggagaaacc atgctggaat tatccgggcc
    15061 attggaaaag acaaccacga cttttctgcc ggtatgttga gcggcatctt tgagggtcat
    15121 gagcagtgca ttataaagcg cggaatcgtc gccattggtg gtctcgcgca caccgcgaat
    15181 gacctgggaa cgatctgaag taagcgaggc ggcgcggtag aagttgcggc tgtaagaata
    15241 aaacgcaacc tggtaggggt gatcgagcga cctcacaaat tccgcaatgg agtcctgagc
    15301 aaatacgaaa cctcgtccgc gaaacatata attgctggtg tcaaacaaga tgaagacgtt
    15361 ggacccgctg acagaagacg cgatagcatc cagagaacca gattgagttc ctccacggat
    15421 taccccattt ggttgcagtt catttggcgc cgtcttgtca ggcggtatgg tttggccgga
    15481 atcctgctca gcgttaagaa tcgtttgggg cgccgcattt ccctcttcaa acgaagccat
    15541 cttttgcgaa atgccgtctt cagccaccgt gaaatcggag ggacgtagag tcgtgatgta
    15601 attccctttt tcatcgctca ccgcgacgtt caattgcacc atggagacga cgacgtgaat
    15661 cacgcttcca tcactgctat tattctggtt ctggtcctgg ttctggttct ggtcctgcgc
    15721 gaagagccag accatgccaa tgggaagaaa aatcaaaaat attaagacga caaacccgta
    15781 tcgttttgcc atatttcccc tcaaaatctt ccccactcgt ctgcgcacat tcagctcgat
    15841 ccctgaaatt tcaggcgact ccttgccaga acttcaaagt cctgccgatg ggctcttctt
    15901 ggctaaggaa gcaatttttc cctgccggaa ttcttctccc gtttcccgca tcgcgcgcaa
    15961 aacggcggac caatcttcca gatgcgaagc gaaagtctgc tccaccatgc ggcgcgtcaa
    16021 ttccgggaca aggatattga gcatcatggg cgaatatccc atctcatccg ccaaccggcc
    16081 ccagtagagg ttccctgaat cccaagactc ggccatcaat cggctcgagt aatagagaaa
    16141 cgtggggaca ggcttcatgc tcatcagctc tcgatgataa gtttgcgcga ttgccgggtg
    16201 cggaactccg aaatcctgag aaatgcggtc caaattagtt tcgatggggt aacgcgtgag
    16261 cacttcatcc aattcgtttc ctgccggtcc ccaggcttgc ctttccgatg aatgattgcg
    16321 acgaaattca gcagccagat aaaaagtatc tgatggagca aggagcgcca gcgcgtcttc
    16381 ttctcgtccc gtgcgcagag cggcctcaat ttgttccagt ctttggggaa gaaggcgctc
    16441 ggaaatgatg ttgacgacct gctcacgcac cttcgcatcc ttggcagcgg agcttagcag
    16501 ttcttcgcca gtgcgttggt agagagccac ggcgtgcatt tcatttcggc tgacgctcca
    16561 ccatcgtggc aatactgcgc tcgtgataag tccgggaacc aaatcttccc agataagcga
    16621 ctggacattt ttgggaacga taaagtcctg ttccacgcgg gccaatgcgt aaggcaaatc
    16681 agccatggac ccgatcaaat gcgcgccgcc gctggccgtc catccgcggc caaatatgcg
    16741 cggggaacgc caaatttgat ccgtggctgt cgcggcttcg ccggaataat cgccgccgga
    16801 aaaatcatgt gaccggacga agagcggatt atggtgaatc atttgtgcgc cgggcggttc
    16861 gtagtaggcg taattgagcc ccactaaagt gtcccgtaaa aaaggagtga gcaaagcgtg
    16921 ggcctgaacc aattccgcag ggctggaggg cgatttgatg attcgaatta agtcggtcct
    16981 catttgcaag gcggtgtggc gattgctgta aagcccgccg gcaaattcat tcctttcgct
    17041 cttggtgaaa agaggtctcg gcagttcgaa ttcgcgcagt tccccggcga ggggcagcaa
    17101 cgactcggcg acttccttac ctttggccat ttggtccagg ccatcgccca gcgaaatcaa
    17161 agtatccagt gaaaccaggc gctggtcatc gatcacctgg tgcatcctcc tggccatttg
    17221 taatcgaagc tgctgtccct ctttggtgta ttgttcaggc cccgcgagca acgcaaagag
    17281 ctggtcttgc ggcaaatatg tttttccgct ggcagcgcgt aaaagttctg caagtgacgc
    17341 gcgccccgcc tgatatacct gatcggaaga atgcagtgtg ctgaaaggaa tgaggacctt
    17401 ttgccaggaa tcattcagtt gggcatctgg aatttcacct tgccgcgcaa gaatttccca
    17461 gaggccaaca tcggcttgca ttattcccag tgcatcggtg cgggcaagat gatcgggaat
    17521 ctgattcagg ccctcggcca ccgtcagaaa gcgcgtgatg gattcattgg tcaacgcggg
    17581 aaattccgca aacatgggat actggtcgcc aaaaagcgga aatttttccg cgagtagcct
    17641 agctgtggga atcgtcagct gctcactcgc cgtccggttg cgatcgattt ccgtgagcat
    17701 taaaaagttg aagactggcc catccggcaa tggatagcga gtaacagcaa ccattccttc
    17761 gatcacttgt tcgggattat tccagctgct tgctttatct ccccactccc ggacaagctt
    17821 ggaatccact tttcggtgca gaacttcctt ccagacatca atgcttccgg gaatgtgcgg
    17881 ctcgccgttg ggattgagga gaagccgcgc ctccagcaga aacagggcag gatcaggccg
    17941 gaaagaatgc ctggtgggca gcggattaat gtcgtgccca cgaagcgcgt catacaaacg
    18001 ctgtattcgg cgagacgaaa tgaaatagac ctgttgggat gagtctacgc gcgaaagcgt
    18061 gtcgtagtag gaggccaccc atcctccatc cttggtgagt agcttgaaga tgaaatccgc
    18121 aggggagcgc ggactcgcgc cgaccagatc cctccaggcg gattccgcct gtgttccccc
    18181 cggtacaagg actcgcccgg accggacact gatttcgctg ccgtaaaagt ccagcacggg
    18241 agcgactaag acgagtttct ttagccctag agattgccac aaaaattggc tggtttccga
    18301 gtccatgcgg gacatcgccc agtagaggcg cgccagattg ggatcccgca agatcgagtc
    18361 aaccacattg cgattggagt ttttcttggt ggaaatccaa tccgaagtct tgtaaagcac
    18421 gggaactttt gtgggtccat aggcagtgtc aaacgacttc cctgcccgca atgcatcttc
    18481 caaatcagcc aaagggaatc cggaattcac cgtcagaaac gcccggttcg catcggccgt
    18541 ctctaatgcg gcgtcgggcc cgcatgtctt ttgcagtctg taaccgagaa tcgcgagaag
    18601 aggcttggca tcctcgcaat ttgaaacatg aatgacttcc tcttttccgg caagcgcttc
    18661 cagttccctg gcctggtcca gataccaatt tacgaggacc aggtactgcg taggcctgcc
    18721 cacctcgtaa ccattcataa cgacattgcg cgcaagcagc ggcagcaatt cgtccgccgt
    18781 aatttgctgg ctgatggcgg ccatccgaag gaacgagcgc atcggccccg ggatggaggc
    18841 ttcgtccaca gtatgaggag ttttggcaga agatctttgc gcaggatcgg caggagccgc
    18901 ggccatggga ggggtcaaca accccagaag caagcacaga aatatgcaat ggaaaaaaag
    18961 gaagaaggca cgatgaaatc tcatagccca gacctccgac aaacctggtc aagaatgact
    19021 attaatactc cgaacggcga agacgaatca ggggcaacac atctcttcgc acggcctccg
    19081 tggaaagtaa aattcgccaa ccttcggcaa tttacactat agaaaggctt gggcgaatcc
    19141 aaatccccgt tcagtctaaa ttccgggcag cgcccgttaa cttcggcaca atcgatatgt
    19201 ttgtccggag aatagaccat tcttcgtagc ccagtcaatg cgcgtgggtc tgggggaagc
    19261 acgccttaag gaacccgctt tgatggccaa tagaaccgac caggccgcgc ctcattttta
    19321 gaaaaattcc ggattgattt ccatcggtat ttcacctgat aaaagcatag ataaataaaa
    19381 gttaattcaa cagcaacata gttgaacttg tactatgacc gctatcagaa gcgatcgatg
    19441 aaaatcgctt ggttctgcca ggtggaatcg ctatgctggc ggctatgaaa attgcatttc
    19501 tgggattggg aagaatgggc gccggaatgg cgcgtaattt gatccgtgcg gggcattcgt
    19561 tggccgttta taaccgcagc cgcgagaaag ctgaggcgct ggccgcggac ggagcgcgcg
    19621 tggcgacctc accagcggat gcggcgcggg acgcagatgc cgtgatgacc atggttgcgg
    19681 acgattacgc gcttgagcag attattttcg gcgaaaatgg cgctacctcc gccatgaagg
    19741 caggttgtat tcacctgtcg catagcacga tcagcacgga catggctcgc agactgactt
    19801 cggagcatgc gcaacggaag caagggtttc tcagcgtccc cgtattcgga cggcctgatg
    19861 cggcggaaag caagaatctg atcgtgatgg ccgcaggacc cgctgacctt gtagaacgct
    19921 gccgtccgct atttgacgcc atagggcgcc aaacatttgt tatgggtacg gagccatggc
    19981 aggcgaatgt cgccaaggta tgcggtaact tcatggtgat tagcgcaatt gaagcgctgg
    20041 gagaggcata tgcgacgctt cggaaatcgg gcgtcgcacc cgagaccttc ctggacgtta
    20101 tgcattctct tttcggctcg caagtgatta ccggctacgg acgcattatt gcccaggaga
    20161 aattcgagcc ggctggattt gccttgaagc tgggattgaa agatgttcgc ctggtactgg
    20221 cggcgtctga ggaggttgcg tctcccatgc cgctcgccag tcttcttcac gatcagttgc
    20281 ttgctggact cgcgcagggg cagggtgaga tggactggtc gagtgtgacg caagtcatag
    20341 ctaggaacgc cgggctggag gcagaatcgt aatcgggtgc ctgtcatttt ggcctttcat
    20401 tgtgaattta gattcgccga atagttgaaa aaccgccggc gggacgccgg cgccacctcg
    20461 taggcaagaa ggagatgcgg atatgtcact cgtggatttt attcctacgc ccaagtttga
    20521 ggactacaag gaacgattca agcagcatta caaaatggaa cgcagcaagg acggagtaat
    20581 cgtcgtggag gcgcacacgg cgggtgggcc tattcaattg agcgtcgaaa atcatcgctc
    20641 ggttggccag ttgttcaaaa cgattggcgc ggatcctgag aatgaagtga tgatcttcac
    20701 cgggtccggc gaagatttca tgatggacgc ggaccccgag gggttcaagc tggagcagga
    20761 ggatttgcag cattgggctt acgagtacgc ttacaaagat gggcgaatca acgtcagcgc
    20821 attagtgaat gacctggaaa ttcccacgat tggcgtgctg aatggtccgg gctttcacac
    20881 ggaaatttgc ctgatgtgcg acatctcgat ttgcagcgaa gacgccgtca ttttcgatct
    20941 gcattatgac atcggctccg ttccgggcga cggcattcac agctgcttcc tggaactctt
    21001 gggcacgaag cgcggagcct atgccctgct caccggccag gcaattgatg cgaaaaaagc
    21061 gctcgaatat gggctggtga atgaggttgt cccgcgagac aggcttctcg atcgcgccaa
    21121 gcagcttgca gcacatatca tgacgcaacc gcgcatcacg cgccggctca ctacccaaat
    21181 tatccggcgt ccctggaaaa aaagaattac ggatgatttg gatggaggct ttggaatcca
    21241 gatgtttgcg cacctggcta agaaaaaatc gattcacagc cggaatcaca ttgctacgtc
    21301 agtggcctac gtgaaagaag gcaagaaaaa taatattaag tagcaagagg cttggctttt
    21361 agtagcccgc gccgcttaaa accctctaat tttattattt gttggatgaa attaatcctg
    21421 cggattcgtt gcaaaaatgg acacttcggg tgcaaaaccg cgatcgtcca tttccagcat
    21481 ggatttcacc acgtacgcga tgtcttccgc tcgtagcttg ctggcattcg ctttttgcgg
    21541 cagcccagcc gtcggataaa aattggtaac cacttcgctc gggttgacca aaatcacgcg
    21601 cacattatat tttcgcaatt ccgctcgcca gcattcggtc atgccacgaa gcgcaaattt
    21661 actggcgtaa tatgcagtgc cgttcggagc tccacgcagt ccggcggtgg aagaaatatt
    21721 cacaatgttt ccgcgctgtt tcttcacgaa atgttttgca gcttcacgcg ccatcaacat
    21781 ggcgcccgtg acgttcgtgg caaacacatc ttcaaattct tttttgccca tatccaccag
    21841 gttcttgaag acgccgctgc ccgcgttgtt taccagaatg tccagatcgc caaatttctg
    21901 cataacttcc ttatagctgc gctcgacatc cgcctcgacc gaaacatccg catgaatcgc
    21961 aagcacgccc gattccttgg ccacgtccgc aagctttcgc tcgtcgcgtc cagtgatcgc
    22021 aacgcgcgcg ccggttgcca caagcgattg cgcgatggct cgcccaatcc cgctgcttcc
    22081 gccggtgatt aacgctgtcg cattcttgag gttcatcttg ttctcccgtg agttgtaatc
    22141 cttggcatag tgccacagcc aggcgagtgg cgtcactgca ggcagttgga tattcgcgaa
    22201 actctgtgat ttccaacgaa agattcgtct atttctcaaa gctttcgaaa tcaatattat
    22261 cctcgcagat caattccatg atgtcccaat ccggccgcga gcgaaacttc atcacggcgt
    22321 tccacggctt cggataggcc ttgggatcgt caaacgtgaa gtcgacctgc aacgtctttt
    22381 catccacacg atgaatgcgc tcgacgatgt gcatctggtc gctctccgga tgtccgaggc
    22441 gatccagcca aagttttccg ttcaggccga ccgtgtctac gaccagcgta tcgccttccc
    22501 agtggccaat ggagtggccg ttataggtgg gcgtcaaatc atccggatgt ttgcggccat
    22561 cgatgtagat gtggcgcaca ttgtggtcat actcgaaaat ttcgataact tccttcggtg
    22621 tctgcacaat tcgcataggg aatatttgca gatagatata cggcataccc ggcggaaaac
    22681 atttataaac actgtctgtc gtttctttta atgagacccc gcgcggtcct tgcgaaggct
    22741 tggcggcatc gaattgcgct tttccccagg gagtcatcgg aggatcgccc ttgctgaatg
    22801 aggcgaagtc atagttgccg cgcatcccat cccatatgcc ggtgatgtcc gtggcggcgg
    22861 acgcgctaat tgtgtttggc gcggagggtg gcgcagacgc ctgctgaggc ttgccttgtc
    22921 cacgagcggc gccagccagg ctcgccatca cactgagcac caggattcct attgcgagcc
    22981 gattccgcat ctgaccactc ctccttattg gccgcttcaa atgagcagct taaatatagc
    23041 gatccaaatt tggaaattta tttaacgcat cgtacgcttc tctgcgtccc gacacaacct
    23101 tggcgtatgg gagacaggaa gaaactcatt tgaaattacg gaatccacct gagcgctttc
    23161 gcaatgttgc tgtggtgcga agattctttt tattgttggg aattcgacgt aaatcacgaa
    23221 acccaaaaag tataaaccag ccagcacaat agcgcgccga ccgccacgag caataaacca
    23281 gagaagcgcc gcttattcag gaagaaaatt agtgtaaggc cactaagaga aaccagtgtc
    23341 atcagaacag ctgaaaaatc aattacatcg gaccaaattt ttccagtgtc ccgcccttta
    23401 tgtaaatcgt tcaacacagc caccaggctg aacgggctga tcgttaaaac ataattcccc
    23461 gttgcgcgat cgatggtcgc gtccgcctcg tagccgggcc ctttaaataa aatttcacac
    23521 tgctctccat ccacgtgaag atcggagaga gcgccctttg tccgatgagt ttgacgaaaa
    23581 tacgttacga gctcgtcttt cgccacgccg tccgggccgc tagcgttcac ccacgcctta
    23641 ttcagagtgc catggtacat agcgggtcgc gcatgactat caaaccactc cgcgtgattt
    23701 aaggtgaggc ccgtgaccgc gaagaacagc agaatagcga aactgaccat ggacaaatag
    23761 gtgtgcagcc agcgcgcaag atgcgcgaag cggcgcttcc agtgcaaata acgcgggcca
    23821 atcgatgacg ggcgcggaga accggatggg tttccgccag ggctactgga tgattttgtg
    23881 gtaatcaatg gtggccgacg caatctctat cccgccagga aactgaaact gtttgggcga
    23941 accgttaaaa tccatctctt ggtgaagcaa ttgataagag ccgtgctcgc gcaccgcttc
    24001 gatcattaca ttatatttgc ccaccttaac cggattgccg gcgtcatcct tgccatccca
    24061 ttggaacgaa tattttccag gagggcgagt ggcgctcgaa acagaattca ggatgtgagt
    24121 gtcttcgctc atggaacgcg tgttttcgtc gcggctccat gcgcgcaatt cattcagata
    24181 tttgtatttc ccaaaccata aggaaaccgt gcgcacggga gcgtggtttt cgttctcaat
    24241 ccatacagca acgtagggcc gatggattcg gtatccctca atcaaactca gctcaatgga
    24301 aacggtcagc acgtatttag gatcccattg ctcggcgccg gattgcacgg aaatcgtctt
    24361 gaacgttgcg gcaggagtgc gcatcgccat gcttggcgca ggcgcagctt catacgcact
    24421 ccaacccgcg ctcacgattc tgtttccgtt tttgtccact agcaaatatt ccacgccggg
    24481 gaccgaagcc gctacgcgag cactttgctc cggcgttaga acggaaaaag ccgtggccaa
    24541 cgcgcccgca accgccggag accgcgcaat gactgttgag ctgatgattt ggtccgcagg
    24601 caaaccagtg cggggatcga caatatgcga gtagtggcgc ccgccaattt ccactccgcg
    24661 ccggtagtct ccacttgtgg ctactgcgcg atcgcgaacg atgaggtgcg caatgggcgt
    24721 tccattttcc gcgtccgatt ttgggtcgga cacgtcgacc ggttcccgcc agtcgccccg
    24781 cacgatcaag tctccgccga tattaataac cagtccctgc gcacccgaag cattgagcgc
    24841 tgcatcggcg gcgcgccctg cgatgtaact tttcacgaaa gagttgagcg cgagtggcgt
    24901 gtcacttgta tgcgtcgccg tctgattcag gggatcgagt gtccaatgaa ctctgcgaac
    24961 acttgcgata gcttcgtcca attcggattg cgaggggatg cgcttctccg cggcagcata
    25021 tttccagacc cgtgtgatcg tctccgcgga aggatcgagg gcgccgccgg tcattccgcg
    25081 atacgtattg aacaaactaa aaacttcaaa taattcggga gaaacgtgga ctggctgacc
    25141 gtgggtgcga aaccaattgc tgaattcgct ctgcggatcc caggaactga gaatgtgcgc
    25201 ctctcgatcg atctcggcca gcgctgcttg ttccgccttt tccgattgcg cctcggacaa
    25261 ggctgaaact tttaattcta gagatgtgcc aagcacattt tcgtaatgaa aaacatagag
    25321 ctttccgcgc gccttggctg ttaatgaacc aaccatgtac agcgaaagcg caacaattcc
    25381 catgattgta aactgggatt tcaccttcgc cacgcgcgac aaaaagttat ctcctttaca
    25441 attactgcca tccgaagttt acactgttga acttatgatt tgcgagcccg accctaacgt
    25501 gaacaatctg ccttagccaa acttcacgca aatagccacg atgaacacat ttgtacgatg
    25561 cagtttgtgg cgccggcgtc tcgcctgcgg ttttgcggtg cgtctcccag acgaaacccg
    25621 ccggcgagat gccggcgcca caaaacctcg acactttttg ggcaccgtgc caacacaatt
    25681 tttcgggtct gccaatcgag agaaagccgg tatacaatgc cgcacaaatc acgggtcaca
    25741 aattcaactg tcatcaagac agcgaggtct aaaaaatgaa acgcgcgatg atgcggttat
    25801 tttttgctgc cacggttgcg gcatttctat tttccggcca ggcgaactta agagcgcagg
    25861 ctactccgtc cgcgcaacac aaggcgaaag ctccgcgctc cgtccggctt tacgttttcg
    25921 attgcggcgt gctgcacaac gcggatatgg gccgttttaa tttgaagaat gaagacgtca
    25981 cgaccagcga tatgtccatg gcttgctttc tgatcgcgca tcccaaggga aatctgatgt
    26041 gggacaccgg cgcgattcct gatgattcct ggaagccgac aggcgcgatg atcacgcaac
    26101 acgtttctct acccgacggc tcatcgcgcg acgccgacgt ggacaagaat cttcgcgatc
    26161 aactgaagga aattgggtac tcccccgccg acatcgacta cctggcgctt tcgcactatc
    26221 actacgatca caccggaaac gccaacgaat ttgcgagcgc tacgtggctt gtgccgcagg
    26281 tagagcatga tgtgatgttc ggcggcgcaa aagtttatgg ctcactatgg ccggccacat
    26341 tttcagcttt gaaaaacagc aagacgatca tccttaagga aaatgagtac gatgtgttcg
    26401 gtgacggcac ggtcatcatc aagtccgctc ccggccacac gcagggtcac caggttcttt
    26461 acgtaaaact ggccaagact ggcggtgttg tgctttctgg cgatctttat cattatcctg
    26521 cggagaaaac tttggaccgc actccgacgt tcgatttcaa cccggaacta actcgcgcca
    26581 gccggaaaga ggttgacgcg tttttgaaga aaactaattc gcagctgtgg attcagcatg
    26641 atttcgcagg caatgccaag ctgaagaagt cgccgcagtt ttacgagtaa ggactggcac
    26701 ttagcggcgc gggcttcagc ctgcggagtc tggttcttgc agggactaaa cgccacagac
    26761 cgaagcccgc gcgactattg cccatcagaa ttaagctgca tgggggaaga tatttatgag
    26821 gaccacagcc gctgtgctct tctccattct tctgggcggt attgttacgc tcggagacag
    26881 cgtcaatgcg atggcgctag ccgcgcacaa ccgtgcaaga ttacgcggtt tgccgcctgg
    26941 catgaagatg cgcgatcagg ctgcgaatgc ttttcgcaag ctccacaacc tccccttttc
    27001 cttgcagctc gaattgaatt tgataattcc cggaaatggc gtgatcgacg gcctctttga
    27061 gtatcatcag cggccttatc acctgccgcg gcagaaccag acttacccag agactgagga
    27121 agattgtaat cgctgaaacg atacttaaaa cccactccgc gcggtgcaga agcttttgcg
    27181 ctcgctcatg atctccctgg acgcgctccc aattttcacc ttcgagcgcg gacgttagac
    27241 cgaataattc gcggctgttt acatccagag caccagcaat ttgccgcagt tttggatcgc
    27301 tcgcctgaat cgttgacatg atttgcgtgt cgaacgagtc cacttgcgca cgaagctgct
    27361 cgacaagctt ggcccggctc ttgcgattat cctgccgcac gagagtgttc aaatctgact
    27421 cgtaattccg taacacggtt tcgagacggt cacgctgtgc ctcacctggc tgcgaaagat
    27481 acgaaaccgc ctcgtcgaat cgctgctggt agaggttaac cgagttcaag gcaatctgga
    27541 tcgtggcttc ttcgtccgga tgcagagtgc ggatttggcg cagaattccg gacgcagccg
    27601 caagcgaatc atgattcgct ttcacgtcgg caggatcatt caggaggaag aagttgcgtt
    27661 ccgctcttcg tgcttcaagc atctggatcg aagcctgctg cgccagcgtt gccgatggcg
    27721 catcgacact cacgatgcga tcgacgatcc agcccatctg aaacaagtaa tagattgcca
    27781 gcagaatgac cggcgcaagt atcagcctta cgacagccag gctgtacgcc accctgcgac
    27841 gaagcgaaac acccggtgcg aaaatccaga acgtcctcga tttcatggcg ctcccccgct
    27901 gggcacactc ctatatttta actggaaatc cgcgttaaac ctccgccttc tgtctcgggt
    27961 attcaccgta tgactggact acaagtcaaa acaggcaaac ccatttttta ctgttgaaaa
    28021 aaatactgcg taaataactg gcttaaatat cgtcaactta ggtgtatgtt atcggcgaca
    28081 cggaaagccc atagcggcat ttccggtttt taactgccat ttcaaaaaca aaagtgtttg
    28141 cgaccggtgc ggcttatatt ccgacacgag gatgagtagc tgtgcgcggc ggaacacagg
    28201 aggacccgat gaaactcgct aggcgaaaaa ttgttgtcgg agcggtggtt ggcgccatga
    28261 ttggcgctac aattttgctg agctgggtaa acacgaaaag ggtgagcgcg gcgcagaccg
    28321 gtcagtgggt cgatcccgca aatccaagtg acgagctcgt ggatgggccg tatcgtgtcg
    28381 atccgaactg gccgaaacca ttgtccacat tatttccgga agaaaaaggc tggacgtggg
    28441 gcgccgtgca gggcgtgttc gcgcagaacc ctgaccgtat tttcgttgtg atgcgtggcg
    28501 agctgcccga tttggcgaat gtgaagaagg cttatgtgga gctcgaaact gcattcggac
    28561 tgaaggcgca cgtcgaagtc cccggccgcg gtatgtatgg aaggaacgca tccgtgggtc
    28621 catacgccag tcctggcgaa gcgtccgatg attatctggg agtcgatgga aaggactatc
    28681 gctggaagca catcattact gtgtatgaca gcaagggaga tgtgaaagag gcgtggactc
    28741 agtgggattc gctctttaag cctgacaatc cgaacgtatt gtcgcagggc cgcgttcata
    28801 agatcctgat cagcccctat gatccggaaa agcgcgtgtg ggccattgac gatggccacg
    28861 aagtgatccg catattcagc aacgacggca agaaattgtt acagaccatc ggcacgctga
    28921 ataccacgac cgaacgcaac gcaaaaatga ccggcaatac cgcgcgcccc tcgtacattc
    28981 agaaaaccgg gccgccgagt gaaactttcg gccgccagac ggatatcgcg tggctgccgg
    29041 acggtacatt cttcgtcacg gacggatatg aagaaacacg cgtcgtaaaa ttcgacaaaa
    29101 acggaaaatt cctgatggcg tggggcgaga gaggtgcggc cggcggtcat gaaatgcggc
    29161 caaattattt caataccgtg cacggcatcg ccatcgacaa tcagcgccgc ctttacatta
    29221 acgatcgcag caaccggcgc attcagatct tcgatgaaaa cggaaaattc cttaatcagt
    29281 ggtaccttgg cgatggcccc actgcgacgt atcacatttt tatgaccgca gaccaacacc
    29341 tctggatgtc cgacggtcac ggcaatttca agttctacaa atatgatctg aacggaaaat
    29401 tgctgtacac atggggcacg atgacgccgg aggcaggcgg cttgtgggga acgcaccagt
    29461 tcggcgtgga tcaggagggc aatctctaca cggcagaagt gtggggcgga cgtccgcaga
    29521 agtttgttcc gcgcaaggga gctgatccgg ctctgttgat cggccagccg attcgcgcgg
    29581 cctggacaaa ataaagaaag tcttcaagcc tcaagaaaag gccaggagaa gaaattttcg
    29641 tccggccttt tttattattg gcgcagtcgg ttgccgcaat ggcttgaaaa atacagggcg
    29701 catgcctgcc tcctacttcg catcaaattc ccgactcaac cacataacct cgagcctcca
    29761 tcctccgcga ccaatgcgca cggacaggtg tatattactt ttcgaaagcc aggcagttta
    29821 gaattcgatt gggatttttt cgggagattt aaatttatga cgaaacgcaa atttacgctt
    29881 ctcatctcag ccgtggcctc gattgcattt gtcgccggcg tgattgccac tagcaaaatg
    29941 atttcggtgc aagcccagac caagccggga tacggatttg cagcgctcgc cggagagcgc
    30001 gtggggcaag acgtggacgg tccataccag gtcgatccca actggccaaa gccaatcacc
    30061 gcgttgcccg gccacgagaa gtggacttat ggagcggtgg aaagcgtctt cgcggaaagc
    30121 ccgaatcgcg tattcattct ggaacgcggc gaaattcctg caatgaagcg gcccgaagag
    30181 attccctacc cctcggttgc tccgagcatt tcgtttcctg tggcccaggt gccctggcga
    30241 aacgcttcgg tcggaccggt aacgtccgcg ggaaatccag tgtggaaagg cacgctaggc
    30301 gtggatgcac gatgggagca ctgcattgtg gtggtcgacg caaatggcaa ccttatcgaa
    30361 gactggacga aatgggacaa gatgctgcgc cgcccgcact ccgtcaccat gaatccgtac
    30421 gatccggaaa agcatatttg ggtggtggac gatcaaggcc acgccgtcta cgaattcacg
    30481 cacgatggaa aagaattggt gaaaactctg ggcactccct accagcccgg cgaggatgac
    30541 aaacatttca accgccccac gttcatcaac tggttgcccg atggaaccat gttcgtgacg
    30601 gacggctatg gcaatacgcg cgtggtgaag tttgataagg acggcaaata cttgacggcg
    30661 tggggccagc catcgaatcc tccggacgac actcgccccg gcactttcca cgatgtgcac
    30721 ggcgttgtgt ttgatgttcc gcgccatcgc gtctttgtca ccgatcgctc cgatcatcgc
    30781 attcagattt tcgacgaaaa cggaaaattt ctcgatcagt ggaccactgg aaaaccgtcc
    30841 actccacaat ttctctataa agcggccgac gggtttttgt ggattgccga cggatacacc
    30901 gcaaaaatta tcaagttcga catggacggc cacctcctat acgaatgggg cagcgagggg
    30961 gactggcccg gcgcgctgtg gaatgtgcac ggcatgagcg ttgatcagga aggaaatctg
    31021 tatcttgctg aagtaaataa cggccgcgcg gaaaaattca agccgcgtcc cggagcgaat
    31081 cctgcgctga tggttggcaa acctttgtat tccgcatgga aataggcaag cactgaatat
    31141 ttatttccat taagtgaatt ttgcgattcg ggtggcgcgc gcttcagtgc tacagaagac
    31201 gctcaccatt ttgagcttgg tagtattttg ccgctgaacc atgagttgtg taacatgtgc
    31261 gcatgcgaat cattacactc gaagaacaca tcactacccc agaaatgcta aaggccgcgc
    31321 agatgcccgc aacgggtgcg tccgcggcgt tcatgcaaac catgaatgcc aagctgctcg
    31381 atgtgggtga aggccgaatt gccgatatgg ttgctgcggg aatcgatatg caagttctat
    31441 ctgtatcggc gaatactgtg gacaaactgg attcagccgc ggcgaatgct gtcgtccgcg
    31501 atgcgaacga tcgaatggcc gcagccgttc gcgcgcatcc cgaccgcttc gcagccttcg
    31561 ctacgcttgc gttgcatgaa ccggacaaag ccgcagcgga actcgatcgc tgcgttcgca
    31621 aattaagatt taaaggtgta atgctgaacg gtgcgagcaa cggccaattt ttcgatcatc
    31681 cgcgattcac ttccattttc gaagccgcaa catcactcga tgtgccgatt tacttgcatc
    31741 ctgcgccgcc gccgaaaccg gttatggaag cgtattacag cggcctcccg ggctccctgg
    31801 gatttatgct ctcaaccgcg atgtggggat ggcacgtgga aacaggaatg cactgcctgc
    31861 gcttaattct tggcggattg tttgaccgtt tccctcaact caaaatcatt gtcggccaca
    31921 tgggagaaaa cctgccattt tccattgctc gcgcggaaat ggttttgggc agaggtgcgg
    31981 cggatttgaa gcgctcggtc ggcgaatatt ttcaagagca tttttatctc acgaccagcg
    32041 gctatttcag cacgccgccg tttcaatgta tgcaacaagt ggtgggaatc gaccggatta
    32101 tgttttccgt ggactatccg ttcagttcaa atacggtggg ccgcaaattt ctggatagtc
    32161 tgccaataag tccggaagag aaggaaagaa tcgcgcatgg caacgcggaa aaacttctga
    32221 aaatctgaat ccccgaaata ttccaatctc caattcatcc tcgctcgtgg tatcttcccc
    32281 actcaatacg ttgggaacca agagtcacaa caaagccgat ttgaaggaga tataaatgaa
    32341 gaaaatcaag cctgtcatcg ccgcgatggc attgtcattg gtaggcgcat atatcacgct
    32401 cgcacaacaa ccggcgcagc aggccccgtc gcagcccatg agctttttta ttacgagcgc
    32461 aggttccggc catggcgcga atcttggcgg gctggctggt gctgaccgca tttgccaaac
    32521 gcttgccaca gccgtcggca gtggaaataa aacatggcac gcctatatga gcaccaatgc
    32581 cgcaaatggc cagcccggtg tgaatgcacg cgatcggatt ggaaatggcc cgtggtacaa
    32641 cgccaaaggt gagaagattg ccaacaatct tgccgatttg catggggaca ccgctgaact
    32701 tgcgcgcctt ggaaataatt tggtgaaaat gtccgcgctc acggaaaaag gcgcgatgct
    32761 caacggccgc ggcgatactc cgaacgtcca tgacatgctg accggatcgc agacggacgg
    32821 cagagcctac acggatggca tggatcacac ctgcagtaac tggacaagcg aaaccaccgg
    32881 cacagctcag cttggacatt ttgaccggaa cggcgcgaac aatacttcgt ggaattccgc
    32941 tcacccgagc cgcggttgca gccaggacaa tctggtgagc acgggcggcg cgggattatt
    33001 ttactgcttc gcgattaatt gagaaatcag cagaatacag aagttccttt aagtagcgcg
    33061 ggcttcagcc tgcgggtttt agtccttccc aggattaaat tctgcaggct gaagagtctc
    33121 gtcgcctttc tgtgcgaagc cctacaaaag taaacgcatt gagtttagac tgagtccgtc
    33181 tcgcccaaag gcttgctatc cctttgcggc ttttacaatc tcgggtggga acaccagtcc
    33241 tagccggtgt gctgcgtcat cattaagaac aatttttttc acgaccactt cctcgaacgg
    33301 catatccttt gggttttcgc ccctgagaac ccgggctgcc aggacgccgc ccgcgtggcc
    33361 ggcttcgtac catccgagcc cgacgctggc caccgccccc cgcatggtga actcaggatc
    33421 gttaataatt aagggaagct tggcatcgcg ggacgccttg acgatgccgt cgaatgcctg
    33481 aagcgcggtg ttatctcccg tcacccaaag tgcttgcacg ccccgatagc taacgacctg
    33541 cgctgcttga aacacctcgc tcgtgctggt cgctgtcact tcctcgagct taatcccccg
    33601 cttcttgaaa gattcgcggg ccacgctgat cactttgacc gaatttgcct ctgagctgtt
    33661 gtaaacggtt cccactgcct tcacgtttgg aacaagtttc tgaatcaggg caacggtgtc
    33721 atccaccggc ggaaacgatc ccactcccgt aatattcgga aggtgatctg tcatgctctt
    33781 acctgcgcct gcggcgattg ggtcgtaaca atatgtaaaa acaacaggcg ttttcttcgc
    33841 aaggttgcag gccgcagcca ggcacggcgt cgtcatcgta ataatcgcgt taagacgctg
    33901 gctgtcataa ttctcaatca gcaacggaat gttggagatt tcaccttgcg cgtgcgcgct
    33961 cagaatctc
//
LOCUS       DASZMB010000220         3025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4349895, whole genome shotgun sequence.
ACCESSION   DASZMB010000220 DASZMB010000000
VERSION     DASZMB010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4349895"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VG272_10370"
     CDS             <1..193
                     /locus_tag="VG272_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14129.1"
                     /translation="GPDGQSGKTIRFEINRFRRDCLLRGVDDIGLALEFERKIRDYEG
                     RHQPAAPLFGPLTYPFTES"
     gene            293..1042
                     /locus_tag="VG272_10375"
     CDS             293..1042
                     /locus_tag="VG272_10375"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWF14130.1"
                     /translation="MRLLQITAILLILGIALLAGPAPQTQVFQTSAGPVKITPLIHAS
                     TLIEADNQIIYVDPARLPSTTGLPPADVIIISDIHGDHMDQTDIAVLSKPTTVIWAPP
                     AVAKTVTTATVIANGETKSWGKWTIEAIPMYNMVRGPEPGKFFHDKGRGNGYVLTFGG
                     KRFYFSGDTEGIPEMRALKNIDVAFVCMNLPNTMSPEEAADAVKAFHPVIAIPYHYRS
                     MKQNSDITIFEKALAGTGIEVRLLNWYPNAP"
     gene            1174..2319
                     /locus_tag="VG272_10380"
     CDS             1174..2319
                     /locus_tag="VG272_10380"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF14131.1"
                     /translation="MKRRNFLAGAAAIGGAALLGNPALSAEPQPSRFKYKKPVIDAHV
                     HWYPPELAELVEKEGAEGGLTNIHRNSKGEVVATQPGYHPYNNNTITFRHEMTDVDLI
                     LKAMDDRDVNMSTLTQTNPHVVWAEPAFGIKMAQTVNNATSALCAKYPQRFTAAITLP
                     MQDVKASIAELERCKNMPGMRAANFPENILGKNLGDPSLWPVYEKCQALNLPIFLHNV
                     DPISERLVEKNITMMNLLGNPFEATIAATSLVLNGVMDEFPTLDIFFPHAGGFFAFVT
                     PRIDWAMAKAPAVIRNGPNGYNGLKLPRASDYRRRFYYDLILHDPQIQRMLIDLVGVD
                     RVVCGTDFPQAMAIMKPVEYVERIPGITQREAEIILCENPARLLRLQ"
     gene            2357..>3025
                     /locus_tag="VG272_10385"
     CDS             2357..>3025
                     /locus_tag="VG272_10385"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HWF14132.1"
                     /translation="MSDIGMMPVDWEVRFDPQRMLRERLQRAKDALNNSDLDALFVFR
                     TEDARYLTGYRHHLGPAFIMGNAVTVLSRNHDPILWTMDFEHCKLRMPWLSADQIRPR
                     ANFREKIGMKRWAEQVEGLVGSLSGKRVGIDIWDINMEKAIREVFPKTEFADGYQSVL
                     MRAKEIKTQDEILCLKIANAMTEAALDKGIRALRPGIKECEILAIIWESMTAMGSEWT
                     QCSNI"
BASE COUNT          728 a          806 c          824 g          667 t
ORIGIN      
        1 aggcccggac ggccagagcg ggaaaaccat tcgctttgag atcaatcgtt ttcgtcgcga
       61 ttgcttgctg cgaggcgtgg acgatatcgg actggcgctt gaatttgagc gcaagattcg
      121 ggattacgag ggacgacatc agcccgcggc tccgcttttc ggtcctttga cctatccatt
      181 tacggaaagc tgaattgcgt ttggcaaatc gccattctaa aaaatatttt gccacaatcc
      241 gcaaacgggg cacgaaccgc agtaaatatt cgatggcaac ttgggggaga aattgaggct
      301 actgcagatt accgctattt tattaatttt gggaattgcg cttcttgctg gacccgcgcc
      361 acagacgcaa gtatttcaga caagcgcagg tccggtaaag atcactccgc tcattcacgc
      421 cagcacgctg atcgaagcgg acaatcaaat tatctacgtt gacccggcaa gacttccgag
      481 cacaaccggt cttccgccgg cggacgtgat cataatcagc gacattcacg gcgaccatat
      541 ggatcagacc gatattgcgg tcctgagcaa accgaccacc gtaatctggg cgccgcctgc
      601 cgtggcaaaa actgttacca cggccacggt aatcgccaac ggcgagacca agagctgggg
      661 caagtggacg attgaagcaa tccccatgta caacatggtt cgcggtcccg agcccggcaa
      721 attttttcat gacaaagggc gcggcaatgg ttacgtgctc acctttggtg gcaagcggtt
      781 ttatttctcc ggtgataccg agggtattcc tgaaatgcgc gcgcttaaga atattgacgt
      841 tgcattcgtt tgcatgaatc tgccgaacac gatgtccccg gaagaagctg cggacgcggt
      901 gaaggcattc caccctgtca tagcgattcc ataccattat cggtccatga aacagaattc
      961 tgacattacg atttttgaaa aggcgttggc cggaactggt attgaggttc gtcttttgaa
     1021 ttggtatccc aacgcgcctt agcctgcgcg aggcaaccga caagagtcac tcgcaagatt
     1081 tcagaaagca tcagcctggc gcgcggtgat ccgggcgagt aatgttcaat agaaccaatc
     1141 ccgaaggcta caatctgtcg gaaggagatc aacatgaaac gtcggaattt tctcgccggt
     1201 gcagcggcga tcggtggggc agcactgctt gggaatcccg ctttgtcagc cgaaccgcag
     1261 cccagccggt tcaaatataa gaagcccgtc atcgatgcgc acgttcattg gtatccgccg
     1321 gaactcgctg agctggtcga aaaagaaggc gccgaaggcg gacttacgaa tattcaccgc
     1381 aatagtaagg gtgaggtggt cgccacgcag cccggatacc atccgtataa taacaataca
     1441 attaccttcc gccatgagat gaccgacgtc gatctgatcc tgaaagcgat ggatgaccgc
     1501 gacgttaaca tgtccactct cacccagacg aacccgcatg ttgtatgggc cgagcctgcg
     1561 ttcggcatca aaatggcgca gaccgtcaac aacgctacgt ccgctttgtg cgcgaagtac
     1621 ccccagcgct tcactgccgc cattactctt ccgatgcagg atgtgaaagc gtcaatcgcc
     1681 gaactggagc gctgcaaaaa catgcccgga atgcgcgctg ccaacttccc tgaaaatatc
     1741 ctggggaaaa atctgggtga cccgtccctg tggccggttt atgaaaaatg ccaggcgctg
     1801 aatcttccga tttttttgca taacgtcgat cccatttctg agcggttggt ggaaaaaaat
     1861 atcaccatga tgaatttgct cggaaatccg tttgaggcca cgatagccgc tacatcgctg
     1921 gtcttgaacg gtgtgatgga tgaatttcca accctggaca tcttttttcc gcacgctggc
     1981 ggtttctttg cgttcgtgac gccgcgcatc gactgggcga tggcgaaagc gccggcagtg
     2041 atccgcaacg gcccgaatgg ctataatgga ctgaaactgc cacgcgccag cgactatcgc
     2101 cgccgcttct actacgacct gattctgcat gatccccaaa ttcagcgcat gctgattgac
     2161 ctggtggggg tggatcgggt cgtatgcggc accgactttc cgcaggcgat ggccatcatg
     2221 aagcctgtag aatatgtcga aaggattccc ggcatcactc agcgcgaggc ggagataatt
     2281 ctttgcgaga atccggctcg cttgttgcgc ctgcagtagg aacctcacgt accttaatta
     2341 aggaaaggaa aactctgtgt ctgatattgg aatgatgccg gtagattggg aagtacgctt
     2401 tgatccgcag cgcatgctgc gggaacgttt gcaacgcgcc aaggacgcgc tgaacaattc
     2461 cgatctggat gcgctgtttg tatttcgcac ggaagacgcg cgctacctga cgggctatcg
     2521 ccatcatctg ggccccgcat ttattatggg caacgcggtt accgtgttgt cgcgcaatca
     2581 cgatccgatt ctttggacca tggacttcga gcactgcaag ctgcgcatgc cgtggctttc
     2641 cgccgaccaa atccgtcccc gcgcgaactt ccgcgaaaaa atcggcatga agcgttgggc
     2701 cgagcaggtc gaaggcctcg tcggttcgct ttccggcaag cgcgtcggca tcgatatctg
     2761 ggacatcaac atggaaaagg cgattcgcga agttttcccc aagaccgaat tcgccgacgg
     2821 ctatcaaagc gtgctgatgc gcgccaagga aattaaaacg caggacgaaa ttctgtgcct
     2881 gaagatcgct aatgcgatga cggaagctgc gctcgataag ggaatccgcg cgttgcgccc
     2941 gggcatcaag gaatgcgaaa ttctggccat catttgggaa tcgatgacgg cgatgggcag
     3001 cgagtggaca cagtgctcga acatt
//
LOCUS       DASZMB010000221        13093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_785337, whole genome shotgun sequence.
ACCESSION   DASZMB010000221 DASZMB010000000
VERSION     DASZMB010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 13093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13093
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785337"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VG272_10390"
     CDS             complement(<1..268)
                     /locus_tag="VG272_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14133.1"
                     /translation="MNETLHFVARHGYLLIFVWVFAEQAGLPIPSGPLLLAAGAISGV
                     HDMNLGTTIGVAVLASLASDSIWYEIGRRKGVRVLQFLCRISLEP"
     gene            complement(261..887)
                     /locus_tag="VG272_10395"
     CDS             complement(261..887)
                     /locus_tag="VG272_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWF14134.1"
                     /translation="MTLKKKAETQFPIHEIIGQRWSSRAFSERPVEPEKLISILEAAR
                     WAASSANEQPWAFLVATGDNRKNYEAMAGVLVDSNRTWAEKAPVLILVFAHTQFAKNG
                     NPNRYALHDVGQAVANLSLQATALGLTVHQMAGFNAEAARERFAAPADWQPVSVIALG
                     YPGDPESLAEKLREREIAERQRKPLDTFVFSGAWGHTAEIPGFPKLNE"
     gene            complement(1065..1643)
                     /locus_tag="VG272_10400"
     CDS             complement(1065..1643)
                     /locus_tag="VG272_10400"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWF14135.1"
                     /translation="MSTPIMAPSQGTKPTQWKLDPAHSAAHFSVRHLMISNVRGEFTR
                     ITGSAAINPADLSQSTIEVEIDANSVNTREPQRDDHLRSADFLDVANYPTLSFRSTQI
                     KSNGPDNLKITGDLTIHGVTKEVTFEAEGPTPPVKDPWGNIRAGITASTKIDRRDFDL
                     RFNGLTEAGGVVVGDEVKITLDAELIQQAGAA"
     gene            complement(1713..2192)
                     /locus_tag="VG272_10405"
     CDS             complement(1713..2192)
                     /locus_tag="VG272_10405"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWF14136.1"
                     /translation="MERNTKTKDDPLALLDHRVFVSLMRTADALSRGAEALLKPYNLS
                     GTQYNVLRILRGAGEKGLACREVGCRLISRDPDITRLLDRMETRGLIVRAREKNDRRV
                     VKTRITAEGLRLLNDLDVPVRKLHRRQLRHLPTKKLQQLSKLLDRAREPFEEPPAEN"
     gene            complement(2380..3099)
                     /locus_tag="VG272_10410"
     CDS             complement(2380..3099)
                     /locus_tag="VG272_10410"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWF14137.1"
                     /translation="MALEKLLTGNVALVTGASRGIGFAIARRLGQMGARVSICGRDAT
                     KLEQSTAALRSEGIDILSVVADVAREDQILSLVQKTQNEFGSIDILVNNAGIGLFGPF
                     HEFSEADWDRVMDTNLKSVFLASRAVAPEMIRRQAGYIINISSLAGKNTFANGAIYCA
                     SKWGLMGLSGSMAEDLRGYGIRVSTICPGSVATEFSGQTKKNPDKALQPDDIAHAVAA
                     LVTQSAGSFISEVHIRPTRKP"
     gene            3369..4706
                     /locus_tag="VG272_10415"
     CDS             3369..4706
                     /locus_tag="VG272_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14138.1"
                     /translation="MIRKWMVTGAGLLWIALGMGSGASKLWAAPQAAQDQAKPAYTLA
                     EYNQYQAADKETNPQQKVKALDDFVKAYPNSSLMPYVYRDYYSTYMALKNFAQVIEYA
                     DRMLALGDKIDTTGRLEAYYTRAQAFFLGAADKTLQTPDALTKARDAAVAGLKTVDDL
                     KNPDPSKADQFEQQKKGVKVLFNAVAALASTNMKDYPAAAGYYKTVLSITPDDPVTHY
                     RLGIVDLQLTPPAATDGFWELARAINLKIPNDPQVRAYLKNQIIRYQQPACDKLVDDQ
                     VNELLTLSATSSDRPASLNVPSAADLQKARDDTANFIPALVAGGDAGKTMWLASCGLE
                     YPDVGVRIMEPPVVEGDTVTLKVFRGATPEEIEAATAANMLVKIDGQPEAKRLEKDAV
                     VRFTGTLTGYTQNPFMLTWDKAKVNAEDLPEEKGAGKKAPAKKSPAKKAPAGD"
     gene            complement(5357..5569)
                     /locus_tag="VG272_10420"
     CDS             complement(5357..5569)
                     /locus_tag="VG272_10420"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWF14139.1"
                     /translation="MKEQLESLVGMMVDRGIHFDEAVNEFEKKFIKRVLDRFHGNQSR
                     AARLLGIHRNTLSRKIDLYKLEHVKK"
     gene            complement(5622..6521)
                     /locus_tag="VG272_10425"
     CDS             complement(5622..6521)
                     /locus_tag="VG272_10425"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF010568.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HWF14140.1"
                     /translation="MASPKLNSVVWETNLPGIHFLNRGKVRDLYEVGGQLLLIATDRL
                     SAFDVVLPTPIPDKGRVLTQLSLFWFNLLRDVIPNHIVTATDFPAELAPFASQLEGRT
                     MLCKRTNPLPIECVVRGYLVGSGWKDYRATGKVCGIALPSGLRESERLPEPIFTPSTK
                     ATTGHDENISFDGAVSLVGAELADKVRSISLEIYRRAAAYAEPRGIILADTKFEFGLL
                     GKELIWIDEALTPDSSRFWPAEGYQPGRAQPSYDKQYVRDYLERIGWNKQPPGPDLPA
                     DIVAATREKYREAYKQLTGQELS"
     gene            complement(6895..10758)
                     /gene="smc"
                     /locus_tag="VG272_10430"
     CDS             complement(6895..10758)
                     /gene="smc"
                     /locus_tag="VG272_10430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HWF14141.1"
                     /translation="MLKLRKVELVGFKSFCEKTQITFSGAGLTCIIGPNGCGKSNIVD
                     AISWVLGEQSHKSLRAERMSDCIFNGTAKRPPLGLAEVTLTLVDPELAEAAEFVLGPQ
                     QGENAEQGPVGVPDSAYGATGASVYNAQDTENAENGDDKSVDGADAPEDALAEGEDSK
                     APTFLKKKRGGAEKHALNLKPGEVVVGRRLYRSGQSEYLINGRVARLRDIQDLFMGIG
                     LGPDSYAIIEQGRIGQILSSKPTDRRAIIEEAAGITKFKTKRRLSEAKLESSKVNLSR
                     VNDIYVEVEKQLASLKRQASKARRYAEIREQMRGLLRQVLASKAKELDMEADRLAGLL
                     GGISQQEAQEAQSVNQWEAEQDRLSSRTYELEGELRQHQNVLGQTALELDRSENRILF
                     NKQRTAELTGRLQQLAGELEQGAAQAAQVETRATMHGESVATIKTEVTRVETILASLT
                     AATAEISGAHHSAEEQIAMLRRTAAQLGEELTRLHGEQAQAEQTLAHHVEGVARFEKQ
                     EQQLLEESLHHRDRSQAADLHWESVEERSHGLDGMVRDAQTRIVDLRHRQQDASKQVG
                     ATRDALAGGRARRASVEQILNERSYTADAVQKLFASSQGSEGTDGFRAVGVLADYAEV
                     GQKYESAIEQFLRDELEYVVVENFDVARAGIAMLRDEVGGRATFFVDSIRKLNLQPNE
                     PIAPFHFENETVARLDRLVEFRDPLGPAAKQFLPRLQSAFLVEQAEVAERLARENPAY
                     SFVTPEGTTYQGRVVSGGRPSETGPLGMKRELRGLDLEVLQFERAASEAQAALQQIEG
                     ELRTSEVTLEQATAQHVESQKLSVAATLQRDQARGDMVRLGIELRDCQAELARLRNGA
                     AAAQQRAEAAQSKRGEIFNARAQAELDIAQGMEQQIALRQTAQTRQEELATRRAEMAG
                     LAERLSAAETIAARIAHELQELVARRQALVLQHDSLIAELGQLAAQTVEYQKQVAALR
                     QEKLRLEARGTELEKEFENTRIRATQMDDTLRHGRQKLGDFREQRSKLEIERARNDSE
                     REHLRQSCVEELNAQPEDLIAEFPALLTGEELRSSDAQYHELKLRIESMGPINMMALE
                     EYNECEQRFEFLGRERSDLLQSITDTQQAIAELDEVCKLKFEEAFNFINANFATAFHT
                     LFGGGTGEMRLSEPDSSGEPGIDVVAQPPGKRLQNVLLLSGGEKAMTALALLIAIFRY
                     QPSPFCILDEVDAPLDEANVSRFTKLVSEMSAGTQFIVVTHNRRTMEMASVLYGVTMQ
                     EPGVSKLVSVRWDEPAAAKAATNAA"
     gene            complement(11138..11482)
                     /locus_tag="VG272_10435"
     CDS             complement(11138..11482)
                     /locus_tag="VG272_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14142.1"
                     /translation="MIRRFLMVTSLAGFLFALSAYAQPAYQNAQSDQQSPKVQTITGK
                     VTQVETGGKAFSVEVTQGSDKKPMRFVVGKETTVQGHVAAGSMASVDYQSDASGQLVA
                     LSVKEQTAQQPQ"
     gene            12201..12836
                     /locus_tag="VG272_10440"
     CDS             12201..12836
                     /locus_tag="VG272_10440"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HWF14143.1"
                     /translation="MLFDFKELPGTECYKLLVSTITPRPIAWVVSQDSKGVLNAGAFS
                     FFNAFSGNPPVIGIGVASYNPARPKDTRVNIRETGQFVVNLVSEANAERMNITAIEFD
                     YGVNELQQAGLTTLPSVRVKPPRIAESPVAMECELMQIVDLGEVGLVLGRVVAMHIHD
                     EMVLDAEKHYIDTPKLKLIGRMHGRGWYARSSDLFEMPRIPVRDWKMKDNL"
BASE COUNT         2813 a         3629 c         3550 g         3101 t
ORIGIN      
        1 caggctcgag tgaaatccga cagagaaatt gaagtacgcg aacgcccttg cgccgcccga
       61 tttcatacca gatggagtca ctagccaaag atgccaaaac cgcaacacct atagtcgtgc
      121 ccaaattcat atcgtgcact cctgaaattg ccccggccgc aagcagtagc ggccccgagg
      181 gtatgggcaa tcccgcctgt tcggcaaaca cccacacgaa tatcaggagg tatccgtgcc
      241 gggctacgaa atgtaaagtt tcattcattc aatttgggga agccgggaat ctcggccgtg
      301 tgcccccatg cgccggaaaa aacgaaggtg tccagcggtt tgcgttgacg ctccgcgatt
      361 tcccgttccc tcaatttttc cgccaacgat tccggatctc ccggatagcc caatgcaatt
      421 acgctcaccg gctgccaatc ggcgggagca gcaaagcgtt cccgcgcagc ctccgcgtta
      481 aaaccggcca tttgatgcac ggtcagaccc agcgccgtcg cctgtagaga caaattggca
      541 acggcttgtc ctacatcgtg gagcgcatac cggtttggat ttccgttctt tgcgaattga
      601 gtatgcgcga aaaccaaaat tagaacgggt gccttctctg cccaggtacg gttcgagtct
      661 accagcactc cggccatggc ttcataattt ttccggttat cgcccgtagc cactagaaaa
      721 gcccaaggct gctcattagc ggaagatgcg gcccagcgcg cggcttccaa aatgctgatc
      781 aatttctccg gctcaacggg acgttccgaa aaagcgcggg aactccagcg ctggccgata
      841 atttcgtgaa tagggaattg cgtttcagct tttttcttaa gagtcatggc gaatttactt
      901 cctttcctga agtgcgtggc ttgtgggcca acggagagag tggggggaat ctgtggccct
      961 gccgatggct gcgcgtgtgt agggacacga tatattgtgt ccctacgaag tgtctcgttt
     1021 caccgcgtcg cgcgcagcga gccgccctcg aatgcgaggt tgatttatgc cgcgcccgct
     1081 tgctgaatca gttccgcatc cagtgtgatt ttcacttcgt ctccgacgac aactccgcct
     1141 gcttcggtca agccattaaa cctaagatcg aaatccctgc gatcgatttt tgtcgacgct
     1201 gtgattccag ctctgatatt gccccaggga tcttttacgg gcggcgttgg accctccgcc
     1261 tcaaatgtca cctctttcgt gacgccgtga atcgtcaggt cgccggtaat tttcaaattg
     1321 tccgggccat tcgatttaat ttgcgtggaa cggaaggaaa gcgttgggta gttcgcgacg
     1381 tctaagaagt cggcgctgcg aagatgatcg tcgcgctgcg gttcgcgtgt attcaccgaa
     1441 ttcgcgtcga tctcgacttc aattgtcgat tggctcaggt cggcggggtt gatcgctgcg
     1501 cttcccgtaa tcctggtgaa ctcgccacga acattcgaaa tcattaaatg ccgtacgcta
     1561 aagtgtgcgg cggaatgcgc agggtcaagt ttccattgtg ttggcttagt tccttgcgat
     1621 ggtgccatga ttggtgtgct catgatcttt tctcctttgc gactagctat cttatgtttg
     1681 attgggaata agcgaatcag gcctcaataa attcaatttt ccgctggtgg ttcttcgaat
     1741 ggttcgcggg cccggtcgag taattttgaa agctgctgaa gctttttcgt gggcaggtgg
     1801 cgcaactggc ggcggtgcaa tttgcgcacc gggacgtcca gatcgttcag cagccgcaat
     1861 ccttccgcag tgatgcgggt cttcacaacg cggcggtcgt ttttttctct cgcgcggaca
     1921 atcaacccac gcgtttccat gcgatcgagc aggcgcgtaa tgtcaggatc gcgcgagatc
     1981 agccggcacc ctacttcgcg gcaagccagc cccttttctc cggctccccg caaaatgcgc
     2041 aggacgttgt attgcgtccc ggaaaggtta tagggcttga gcaatgcctc ggcgccacgg
     2101 ctcagcgcgt cagcggtgcg catgaggctg acgaaaacgc ggtggtcgag caaggccagc
     2161 ggatcgtctt tcgtctttgt gtttcgttcc atgagctaat aatagtcgtg ataacgacta
     2221 ttgtcaagag gaatgtttag gcgcaattct cagcctttct cctcttttct ctgtgtcctc
     2281 tgtgttaaag gtttggcctt ggctcgggga agagctttaa cacagaggac atagagaaaa
     2341 gacgagaggc ccggagagtt ttgcggatga gcttttttgt tacggtttgc gcgtggggcg
     2401 gatgtgcact tcgctgataa agcttccggc ggattgggtt acaagcgcgg ccaccgcgtg
     2461 cgcgatgtca tcgggttgca gtgccttgtc cggatttttc ttggtttgtc ccgaaaattc
     2521 cgtggccacc gagccggggc agatggtgct aacgcgaatc ccgtaaccgc gcaaatcttc
     2581 ggccatgctt cctgaaaggc ccatcagtcc ccatttcgat gcgcaataaa ttgcgccgtt
     2641 cgcgaatgta ttttttcccg cgagcgagct gatgttgatg atgtagccag cctgtctccg
     2701 aatcatttcc ggcgcaaccg cgcgagacgc gagaaaaacg gacttcaaat ttgtatccat
     2761 cacccggtcc caatcggcct cgctgaattc atggaacggg ccaaacaagc cgatgccggc
     2821 gttattcacc aaaatgtcga tggagccaaa ttcattttgt gttttttgaa caagcgacaa
     2881 aatctggtct tcgcgcgcca catccgccac gacggataga atgtcaattc cttcgctgcg
     2941 aagcgccgcc gtcgattgtt cgagtttcgt ggcgtcgcgc ccgcagatcg acacgcgtgc
     3001 ccccatctgg cccaggcgcc gcgcaatcgc gaaacctatt ccgcggctcg ctcccgtaac
     3061 gagcgcgaca ttgccagtta agagtttttc cagggccatt gtttctcctt tactttaccg
     3121 gcggcaaaaa tgatattctc aaccgaggct tccttactcc ggagtttgtc atcataacaa
     3181 tagcccaaaa gatgaacgat gcactcccga atcggagagc cttgacttag aagcggttgc
     3241 ggaagggaat cacgcttgcg ccatggagta ggattcccta taatccgata gctttaagcc
     3301 aaatgacatt ctttcaaact gtttttgcga tggatttctt tgaaactcgc tgaggaggct
     3361 ggaagccgat gatacgaaag tggatggtga cgggcgccgg tctattatgg atcgcgctgg
     3421 gaatgggatc gggcgcaagt aagttgtggg ccgcaccaca ggcggcgcag gatcaggcaa
     3481 agcccgcgta caccctggct gaatacaatc aataccaggc ggcggataaa gaaacgaacc
     3541 ctcagcaaaa agtaaaagcg cttgatgatt ttgtgaaggc ctacccgaac tcctccttaa
     3601 tgccttatgt ttaccgggat tattactcaa cttacatggc gctgaaaaat ttcgcccagg
     3661 tgattgagta cgccgaccgg atgcttgctc ttggcgacaa gatcgacacc acgggacggc
     3721 tcgaagcgta ttacacgcgc gcgcaggcat ttttcctggg ggccgcggat aagacactgc
     3781 aaactcccga tgcgctgacg aaagcgcggg acgctgctgt tgccggtctg aagactgtcg
     3841 atgacctgaa gaatcccgat ccatcgaagg ccgaccagtt cgagcagcag aagaagggcg
     3901 tcaaggtttt gttcaacgcc gtagccgctc tcgcttccac caatatgaag gattaccccg
     3961 cagctgccgg atactacaag acggtgttgt ccattactcc tgacgatccc gttacgcact
     4021 accggttggg catcgtggat ttgcagctga ctccgcccgc ggccaccgat ggtttctggg
     4081 aacttgcccg cgccattaac ctgaagattc ccaacgatcc gcaagtgcgc gcctatctga
     4141 agaatcagat cattcgttac cagcagcccg catgcgacaa gctggtggac gatcaggtga
     4201 acgaactgct cacgctgtcc gccaccagtt ccgatcgtcc cgcatcgttg aacgttccaa
     4261 gcgcggcgga cttgcaaaag gctcgcgatg atactgcgaa cttcattcct gcgctggtgg
     4321 caggcggtga tgcaggaaag accatgtggc ttgccagctg cggtctcgaa tatcctgacg
     4381 tcggtgtgcg tatcatggaa ccgcccgtgg ttgagggcga cacggtgacg ctgaaggtat
     4441 tccgcggcgc aacgcccgag gaaatcgaag cagccaccgc tgccaacatg ctagtaaaga
     4501 tcgatggtca acccgaagcg aagcgcctcg aaaaagacgc ggtcgttcga ttcaccggca
     4561 cgttgaccgg atacacgcag aatcccttca tgctcacgtg ggataaagcc aaggtcaacg
     4621 cggaagattt gcctgaggag aagggcgcgg gcaagaaggc ccctgccaag aaatctccag
     4681 cgaaaaaagc tcccgcaggc gactagtatt cccacaaacc tgaatgatac atggccccgg
     4741 gaaattttcc ggggccattt ttttgtagcg ttggcaagac gccggcgcca cgaggactgc
     4801 gtccctatgg aagtgcggaa atcattgttg ctggagaagt gactgttcgt gcggagaaag
     4861 atcgctcggt tctgcttctt tcaaattttc tctcgtctat agcctgaatg acggggcaaa
     4921 gtgctaaaga aaactaaact atggaatatc aattgagggc aataaataag cactaagcta
     4981 gagacaactt gtaaccgttc gcaattcgcg agtctccaat cactagaggt catctcataa
     5041 atagcctttt tcttgtttca caaggtgaaa gtaaggccac ggtctcgcgc caacggcgcg
     5101 aagaatgcca gcccggggca acgcccaggg gaccgtggct aactacagtt tccgccctga
     5161 aagggcggaa gggccctgcg ccctttcagg gcgctgcaga atcgaatcgt tgccaaaccc
     5221 agggcgttgc cctgggctag cgtacgacgg cgctttcagc gccggaagaa gcggatcttt
     5281 ttgaagaacc tgttgattgc ttttcgataa tggaatgcag ccatttatga gatagcttct
     5341 agccactagt ccctcgtcac ttcttcacgt gttccagttt gtacaagtca atcttgcggc
     5401 tcagcgtatt gcggtgaata ccaagcaggc gggcagctcg cgactggttg ccatgaaaac
     5461 ggtccagcac gcgtttaata aatttttttt caaattcatt tacagcttcg tcgaagtgaa
     5521 tcccgcgatc gaccatcatt cctaccagcg attccaattg ctccttcacc gtagcacgct
     5581 ccgcatgaaa ttgttcgctg ccggaagaat ttccggcgcg attagctgag ttcctggccc
     5641 gttagctgct tgtacgcttc cctgtatttc tcgcgagtcg cggcgacaat atcggcaggg
     5701 agatccggtc cgggtggctg cttattccat ccgatccgtt cgagatagtc gcggacgtat
     5761 tgcttatcgt acgagggctg cgcgcggccg ggctggtagc cttccgctgg ccagaagcgt
     5821 gaagaatccg gagtcaaagc ctcgtcaatc cagatcaatt cttttcctaa caggccaaat
     5881 tcaaattttg tgtccgccag aataattccg cgaggctcgg cataggccgc agctcggcgg
     5941 taaatttcca gactgatcga gcgcactttg tccgccagct ccgctccgac tagcgacaca
     6001 gcgccatcga acgaaatatt ttcgtcgtgc ccggtggtgg ccttggtcga tggcgtaaaa
     6061 atcggctccg gcaggcgctc cgattctcga aggccggagg gaagcgcaat cccgcaaact
     6121 tttccggtgg cgcgataatc cttccagcct gagccgacaa gatatccgcg cacaacgcac
     6181 tcgatcggca gcggatttgt ccgcttgcaa agcatcgtac ggccctcgag ctgcgatgcg
     6241 aagggcgcca gttcagccgg aaaatccgta gccgtcacaa tatgattcgg gatcacatcg
     6301 cgcagcagat tgaaccagaa aagcgaaagc tgcgtaagca cgcgcccctt gtccgggatt
     6361 ggagtgggca gcacgacatc gaacgcggat agccggtccg tggcgatcag cagaagctgg
     6421 ccaccgactt catatagatc gcgtactttc ccgcgattta ggaaatgaat gcctggtaaa
     6481 tttgtttccc aaactactga atttaatttt ggagaagcca cagataacct atcccttcag
     6541 agaggccgtc attctagcct acgcgtcagc gatgtcaacg gagaagaaaa gcgaaaagca
     6601 agaatcgcat gtggaaaaaa taaaaaagtg tgcaaaattt ttcacttccc gtttggctgg
     6661 aaaaataaat tggattcagc tgttcccgag tcttgggcga ttgggggctt tcgcggcgcc
     6721 ggcgggttta ggtgtgcggt gacgttacaa aaaaccgctc gcgagacgcc ggcgccacga
     6781 aacgcgggct cctcgcctta aaaattgaaa atatttaacg tagtgggcac aaaaaagagg
     6841 agaaaagcct gataactttt ctccccttga agttctaatt tttatgaagc tactttatgc
     6901 ggcgttggtt gcggctttcg cagcggccgg ctcatcccaa cgcacagaca caagtttcga
     6961 gacgcccggc tcctgcatgg tcactccata caagaccgac gccatctcca tcgttctccg
     7021 attgtgcgtc acgacgatga actgagtgcc cgcgctcatc tcggaaacaa gtttcgtgaa
     7081 gcggctaacg ttcgcttcgt cgagcggcgc atcgacttcg tccagaatgc agaatgggct
     7141 tggctgatag cggaagatgg cgatgagcag cgccagggct gtcatggctt tttctccgcc
     7201 cgaaagcagc agcacgttct gcaaacgctt cccgggcggc tgcgccacca catcgatacc
     7261 cggctcgccg gagctgtccg gttcggacaa cctcatctcg cccgtgccgc ctccgaaaag
     7321 tgtgtggaag gcggtcgcga aatttgcgtt gatgaagttg aatgcttcct cgaatttcag
     7381 tttgcagact tcatcgagtt cggcgatggc ctgctgcgtg tccgtgatcg attgcagcag
     7441 gtcgctccgc tcgcggccga ggaattcaaa gcgctgttcg cattcgttgt attcctcgag
     7501 cgccatcatg ttgatggggc ccatggactc gattcgcaat ttcaattcgt gatattgcgc
     7561 gtcggaagag cgcagttcct cgcccgtcag gagagcgggg aattccgcaa tcaaatcttc
     7621 cggctgcgcg ttcaactcct cgacgcacga ttgccgcagg tgctcgcgtt cggaatcatt
     7681 gcgcgcacgt tcgatttcca gtttgctgcg ctgttcgcgg aaatcgccga gcttttggcg
     7741 cccgtggcgt agcgtgtcgt ccatttgcgt ggcgcgaatg cgcgtgttct cgaactcctt
     7801 ttcgagttcg gtgccgcgcg cttcgagccg caatttttcc tggcgcagtg ccgcgacctg
     7861 tttctgatat tccacggtct gtgccgcgag ttgtcccagt tccgcaatga gcgaatcgtg
     7921 ttgcaggaca agcgcctgcc gccgcgcgac aagttcctgc aattcgtgcg caatgcgagc
     7981 cgcaatcgtt tccgcggcag aaaggcgttc cgccaagccg gccatttccg cgcgccgcgt
     8041 tgctaattct tcctgcctcg tctgcgccgt ttgccgcaaa gcgatttgct gctccatacc
     8101 ctgagcgatg tccagttccg cctgcgctcg cgcattgaaa atttcgccgc gcttcgattg
     8161 tgcggcttcg gcgcgttgct gggctgcggc tgcgccgttt cgcagccgcg cgagttccgc
     8221 ttggcagtca cgcaattcga ttccgagtcg aaccatatcg cctcgcgcct gatcgcgttg
     8281 cagcgtggcc gctaccgaaa gtttttggga ctcgacgtgc tgcgccgtgg cctgttcgag
     8341 agtgacttca ctcgtgcgca gctctccttc gatttgttgg agggcagctt gcgcttcgga
     8401 cgccgcgcgt tcaaattgca gtacctcgag gtccagcccg cgcaactctc gcttcatgcc
     8461 cagcggcccg gtttccgaag gacgcccgcc gctcaccacg cggccctgat acgtcgtccc
     8521 ctcgggagtc acgaaagaat atgcgggatt ctcgcgagcg agacgctcgg ccacttcggc
     8581 ctgctcgacg aggaacgccg attgcaatcg cggcaggaac tgctttgccg ccggacccag
     8641 cggatcgcgg aactccacca agcggtcgag tcgcgcaacg gtttcgtttt caaaatgaaa
     8701 gggcgcgatt ggctcgttcg gttgcaaatt cagcttgcga atcgaatcca cgaagaaggt
     8761 ggcgcgtcca ccaacttcgt cgcgcagcat ggcaattcca gcgcgagcaa cgtcgaaatt
     8821 ttcgacgaca acatattcca gttcgtcgcg caaaaattgt tcgatcgcgc tttcgtactt
     8881 ttgtccgact tcggcgtaat cggcgaggac tccaaccgcg cggaagccgt cggttccctc
     8941 gctgccctgg ctcgaagcaa aaagtttctg taccgcgtcg gcagtgtagg agcgctcatt
     9001 taaaatttgc tcgaccgatg cgcggcgtgc acggcctccc gcgagagcat cgcgcgttgc
     9061 gcccacttgt ttggaagcat cctgctgccg gtgacgaagg tcgacgatgc gcgtttgcgc
     9121 atcgcgtacc atcccatcga ggccgtgcga gcgttcctct acagattccc agtgcagatc
     9181 ggcggcctgc gaacggtcgc gatgatgcaa ggattcttcg agcagttgct gttcctgttt
     9241 ctcaaaacgc gcgacgcctt ccacgtggtg tgccaacgtt tgttcggcct gagcctgctc
     9301 tccgtgcaag cgcgtgagtt cttcgcccag ttgagcagcc gtacggcgca gcatcgcaat
     9361 ttgctcttcg gcggaatggt gcgcgccgga aatttcagcg gtggctgcgg tcaggcttgc
     9421 cagaatcgtt tcgacgcgcg tcacttcggt cttgatggtt gcaacggact cgccatgcat
     9481 ggtggcgcgt gtttcaacct gcgcggcctg tgccgcgcct tgctcaagtt cccctgccaa
     9541 ttgctgcagg cggccggtca actccgccgt gcgctgcttg ttaaacagaa tacgattttc
     9601 gctgcgatcg agttccagcg ccgtttgtcc gagcacgttt tgatgctgcc ggagttcgcc
     9661 ttcgagttcg taagtgcgtg aagagagccg gtcttgctct gcttcccatt ggttaacaga
     9721 ctgcgcttcc tgcgcttcct gctgtgaaat tccgccaagc agtccggcca agcggtccgc
     9781 ttccatgtcg agctctttgg ctttgctcgc cagaacctgt cgcagcaaac ctctcatctg
     9841 ctcgcgaatt tccgcgtagc gccgcgcctt cgaagcttgc cgcttcaggg acgcgagttg
     9901 cttctcgact tcaacataaa tgtcattcac gcgcgaaaga tttactttgg acgattccag
     9961 tttcgcttcg gagagccggc gcttggtttt aaatttagtg attccggcgg cttcttcgat
    10021 gatggcgcgc cggtccgttg gcttggagct gagaatctgg ccgatgcggc cctgctcgat
    10081 gatcgcgtaa gaatccggcc cgaggccgat gcccatgaac aaatcctgaa tatcgcgcag
    10141 gcgcgccacg cgaccgttga tcagatactc gctctggccg gagcggtaca agcgtctgcc
    10201 aacgaccact tctccgggtt ttaaattcaa cgcgtgcttc tccgcgccac cgcgcttctt
    10261 cttcaaaaat gtaggcgcct tcgaatcttc gccctctgcg agtgcgtcct ccggggcgtc
    10321 agcgccatcc acagatttat cgtcgccgtt ctctgcgttc tcagtgtcct gtgcgttata
    10381 aacgcttgcc ccagtcgcgc cataagcact gtctggcacg cctacaggtc cctgctccgc
    10441 gttctcgccc tgctgaggac caagaacaaa ctccgctgcc tcggcgagct ccggatcaac
    10501 gagcgtcagc gtgacttccg cgagcccaag aggaggacgc ttcgcggtcc cattaaaaat
    10561 acagtcggac atacgctcgg cgcgcagcga cttgtggctc tgctcgccca agacccatga
    10621 aatggcgtcg acaatattgg acttgccgca gccattgggg ccgatgatgc acgtaagccc
    10681 ggcgccgcta aaagtgattt gcgttttttc gcaaaatgat ttgaatccga caagttcgac
    10741 tttacgcagt tttagcaaga tatccccttt aaggcctccg ccgtgtgcgc gccgggcgat
    10801 cctgtcgctg aaaatcgctt acaccacgcc ctttgcacat acatcggaag agacgtattc
    10861 cgcttactcg ggagggttcg agccgcgaca atttcgctga agggtcgttt agcagaggcc
    10921 catccgcctg gcgctacgat ggcggttacg ggaaaacaaa ttatcacgcg atgaggttga
    10981 agtaaagacg caagcgcata ttaatcaaca ttttttcagg tgttgcggcg aagcgcgagc
    11041 gcctggaaaa ccgaattttg aagacgcaaa aagaccaagg cggaaccggg aataagctcc
    11101 cgatcccgcc ccctaaattt tttcggatta ttccccgtta ctgcggttgt tgcgccgttt
    11161 gttcctttac ggagagcgct acgagctggc cgcttgcgtc agattgataa tcgacgctgg
    11221 ccatcgatcc ggcggccacg tgcccttgca cggtcgtctc cttgcctaca acgaaccgca
    11281 tcggcttctt gtcgctgccc tgggttacct cgaccgagaa cgcctttccc ccagtctcca
    11341 cctgagtgac tttccctgta atcgtttgca ccttcggaga ttgctggtcg gattgcgcat
    11401 tctgatatgc gggttgtgcg tatgcggata gagcgaataa gaaccctgcg agcgacgtaa
    11461 ccattagaaa tcttcgaatc atcggccttt acctccagaa atatgggagg gcacggagtc
    11521 tcgtgcacat gcgtgtccac tctgcgaaat attttctgca aggcacgtga gtgtcgcaga
    11581 actctttccc accgttattt cctatatcaa ttggcataag gcgacatgag tagctggaag
    11641 atttttcgcg cgccatgcag aaaatttcta aagggaatcg ggtagaagcc atccagcatc
    11701 aatatgctta ctcgtatcca gatcattctt ggattttgta taaatggttt tcgattttgt
    11761 gggttgcggc ttcagaggct gcggaaaaac tcaacatttt gtcattccga gcgaagcgag
    11821 gaatccctcc ttctttattt tcttatactt aaatcgaaga gagattcctc gcttcgctcg
    11881 gaatgacgga ataaactact ttttccgcaa actgttcaac cgcgacatca aaaacagcaa
    11941 gaatcaccgg gctttagccc ctggcagcct aacgagtttg ggcggctttc aattcggaag
    12001 ttagtttcta catgttttcg caataccatt ccaacacagc ccgtagcatg ggtatttcgt
    12061 gccaatcgta cagggccata agcgttgagc gaaccgtcac gatgttttgt tatagtcccg
    12121 tattcgctgt tgtcctatga caagcgacga ttcgcggagt ggccgagcgc ttttttgatc
    12181 tgatcacggg aggattttca atgctattcg attttaagga attgccaggt acggaatgct
    12241 acaagttgct ggtttcgacg ataaccccgc gacctatcgc ttgggttgtc agccaggatt
    12301 ccaaaggagt gttgaacgct ggagcgtttt ccttcttcaa cgctttttcc ggcaaccctc
    12361 ctgtcattgg cattggcgtt gccagctata accccgctcg accgaaagat acgcgcgtaa
    12421 acattcgcga aacaggccag ttcgtagtga atctcgtctc cgaagcaaac gccgagcgga
    12481 tgaatatcac cgcgatcgaa ttcgattatg gtgtgaatga gttgcaacaa gccgggctca
    12541 ccactctgcc gtcggtgcgg gtgaagccgc cccgcatcgc cgaaagccct gtcgccatgg
    12601 agtgtgagtt gatgcaaatc gtggatttgg gcgaggttgg actggttctg ggccgggtgg
    12661 tagccatgca catccatgac gaaatggtgc tcgacgcgga gaaacattac atcgacacac
    12721 caaaattgaa actcataggc cgcatgcatg gccgtggatg gtacgcccga tcttcagatc
    12781 tattcgagat gccacgcatt cccgtgcgcg actggaagat gaaagataat ctgtaaggcc
    12841 ataggttttt gtggcagagg cttcagcctg tggaatttgg atcttgcaaa ggactgaccc
    12901 ccgtaggctg aagagtctgt gtgagaagtg caaaattgaa gcttcagcgg caaaagaggc
    12961 tgcggaaaaa atcaacattt tgtcattccg agcgaagcga ggaatccctc cttctttatt
    13021 ttcttatact taaatcgaag agagattcct cgcttcgctc ggaatgacag aataaactac
    13081 tttttccgca aac
//
LOCUS       DASZMB010000222         4323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_785404, whole genome shotgun sequence.
ACCESSION   DASZMB010000222 DASZMB010000000
VERSION     DASZMB010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785404"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(301..1422)
                     /locus_tag="VG272_10445"
     CDS             complement(301..1422)
                     /locus_tag="VG272_10445"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HWF14144.1"
                     /translation="MKSRPNSFQFLSLLLCLSIAGSLPISAAPAPRATRTAHVTHPIV
                     KKYSSSRYGVPTFTDSEKGDVATYDDPIVRTAAVDALGRYNGSVVAIDPTTGRILTIV
                     NQGLAFSAGFIPCSTIKPVIAVAALQEGVVTRDTMIRVAPRKYLNLTEALAHSNNAFF
                     EELGRRMGFGTVSHYGRLLGLGELAGYQIPEEHPGGFPTEPPAFGGVARMSSFGQGIQ
                     MTPLQLGALVSSFANGGTLYYLQYPRTPEERANFAPRVKRQLNIESLLPEVRDGMLAA
                     VLYGTARSSYISDDEQALGKTGTCSDSASRLGWFVSYADQSHPKIVLVVLMRGHTMAI
                     KGAMAAGVAGRIYRRLNDENYFLVKNNEPHAALVATTDR"
     gene            complement(1756..2880)
                     /gene="recA"
                     /locus_tag="VG272_10450"
     CDS             complement(1756..2880)
                     /gene="recA"
                     /locus_tag="VG272_10450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HWF14145.1"
                     /translation="MTDDKSKMLETAISQIEKDYGKGAIMRLGSRDVLVPVTVIPTGA
                     LSIDAALGVGGVPRGRVIEIYGPESGGKTTLTLHIIAEAQKLGGQAAFIDAEHALDPV
                     YARKLGVDVDNLLVSQPDNGEQALEIAQKLIQSNAVDVIVVDSVAALVPRAELEGDMG
                     DPQMGLQARLMSQALRKLTAIVSKSNTCLIFINQIREKIGVMFGNPETTTGGRALKFY
                     SSIRLDIRRIQSIKEGDRVIGSRTRAKVVKNKVAAPFREAEFDILYGEGVSREGDLID
                     LGVEKNVLEKSGTWISYGGERLGQGRENVRIFLKEHADVRRKLENDLRKKMGIAIPGA
                     ANGNSLPVAHAEKTAERPVAQAAVAAASAPAAANKPRPTK"
     gene            complement(3202..4068)
                     /locus_tag="VG272_10455"
     CDS             complement(3202..4068)
                     /locus_tag="VG272_10455"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: protein motif:HMM:NF004474.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HWF14146.1"
                     /translation="MTLDAIRTVAVLGAGTMGNGIAHVLARCGYEVILRDVDESFLAR
                     GRDTIAKNLDREAKKGKIAEEDKSKILARIRTTTDLSEIARADFAVEAVPERLDLKRS
                     VLREADKLLRPDVILASNTSSISVTTLAAATTRPERFIGMHFMNPVPVMVLIEVIRAL
                     QTSDETFQTTMALCERLEKKPVAVNDAPGFVSNRVLLPMINEAAFSVMEGVATAESVD
                     SIMKLGMNHPMGPLELADFIGLDVCVHILDVLHEGFGDSKYRACPLLRKMVAAGWLGK
                     KSGRGFYTYAAK"
BASE COUNT          996 a         1156 c         1175 g          996 t
ORIGIN      
        1 gggcgacctc gacaagttgc tgcacgcggg cgatacatgg gaagtgcgct agaatttctc
       61 acccacgcgc atagctgaat ttaacaagct attgattatt ttaaagggcg gcctgcaatg
      121 cgggccgccc ttttattttg ggcttaaata attcagaggt gtgaatttga gacatggaaa
      181 agcgatgaat taattctgaa aatttcaaat ttaaaatgat ttaaaatatc aaatttgaag
      241 tcgaatcctg gaaataatcc tgcaatttat atttcaaagt ttagagttgc cggccaacta
      301 ctagcggtct gtagtggcga ctagggctgc gtgcggctcg ttgttcttca ccaggaagta
      361 attctcatcg ttcaggcggc gataaatgcg tcctgcaaca ccggcagcca tggctccctt
      421 gatcgccatg gtgtgcccgc gcatgaggac aaccaggaca atcttcggat gagattgatc
      481 cgcataggaa acaaaccatc cgagtcgcga agcggaatcc gaacacgttc ctgtttttcc
      541 aagcgcttgc tcatcgtcgg aaatgtagct ggagcgcgcg gtgccataga gcacggcggc
      601 gagcatgccg tcgcgcactt caggcaaaag cgattcgata ttcagctggc gctttacgcg
      661 cggcgcgaaa ttcgcgcgtt cttccggggt cctgggatat tgcaaataat agagcgtccc
      721 gccgttggca aagctggaaa cgagcgcgcc aagttgcagc ggcgtcatct gaattccctg
      781 tccaaagctg gacatccggg ccacaccgcc aaaggctggc ggctcggtcg ggaatcctcc
      841 gggatgctct tcgggaatct ggtagccggc aagttctcca agacccagca ggcgtccgta
      901 gtgcgaaacg gtaccaaatc ccatgcggcg gcctagttct tcaaagaagg cgttattgga
      961 atgcgccaaa gcttccgtga ggttcagata ttttcgggga gcaacgcgga tcatggtgtc
     1021 gcgcgtgact acgccctctt gcagggcggc aaccgcgatc accggcttaa tcgtcgagca
     1081 aggaatgaag cccgcggaaa acgcgagacc ttgatttacg atcgtgagga tgcggccagt
     1141 cgtaggatcg atcgcgacga cagaaccgtt gtaacggccc aatgcatcca cggcagccgt
     1201 gcgtacaatc ggatcgtcat acgtcgcgac atcgcccttt tcggagtccg taaacgtggg
     1261 gacgccgtag cgcgatgaag aatatttttt tactatcgga tgagtgacgt gagcagtgcg
     1321 cgtggcgcgc ggagcaggag cagccgaaat gggtaagctt cctgcaattg ataagcagag
     1381 aagcaaggag agaaactgaa aagaattggg ccgtgatttc atgtgaaccg gttccgatct
     1441 ggaccgctta agcgctcccg atgcgcgagt taaagctgag ccccgattct aggtaattta
     1501 catgactgaa ggcaatagct attccgtcct attacgtact atcggccact atcccagaca
     1561 ggtacttgcc gtaccaagct gaaacgcgcg taatgaaaac acgccacttc ggttttttgt
     1621 gaccgatggg ccgcgggact atcgctggcg gcgctcgaat aatcttcacc aaaaaaacat
     1681 attagaaata actgcgcggc ctggagcatc ccaggccgcg gtcaggttta aagcgagtcc
     1741 cattttcagc aatgattatt tcgttgggcg tggcttgttt gccgctgcgg gagcggaagc
     1801 tgccgcaaca gctgcctgag ccaccggcct ttctgcggtt ttctccgcat gagctaccgg
     1861 cagagagttg ccatttgccg cacccggaat ggcaatgccc attttctttc tgagatcgtt
     1921 ctccagtttt cgacgaacgt cggcatgctc tttcaagaag attcgaacgt tttcgcggcc
     1981 ctggcccaaa cgctctccgc cgtaggaaat ccacgtgccg gatttttcga ggacgttctt
     2041 ttccacgccc aaatcgatca agtcgccttc acgggaaacc ccttcgccgt aaagaatgtc
     2101 aaactcagcc tcgcggaagg gcgcggccac tttattcttc actacttttg cgcgtgtgcg
     2161 cgaaccgatg acgcggtcgc cttctttaat ggattgaatg cggcggatat cgagacgaat
     2221 cgaggagtaa aatttcagtg cgcggccgcc ggttgtggtt tcgggattac cgaacatcac
     2281 gccgattttt tcgcgaatct ggttaatgaa aatcaggcag gtgttcgact tcgatacgat
     2341 ggccgttagc tttcgcagcg cttgcgacat caggcgcgct tgcaagccca tctgcggatc
     2401 gcccatatcg ccttcgagtt cggcgcgcgg aacgagcgcc gcgacggagt ccaccacgat
     2461 tacgtcaacc gcattggact gaatcagctt ctgcgcaatt tccagcgctt gctcaccatt
     2521 gtccggctgc gacacgagca ggttgtccac gtccacgcca agctttcgcg cgtaaaccgg
     2581 atcgagcgcg tgctccgcat cgatgaacgc cgcctggccg cccagttttt gagcctcggc
     2641 aataatatgc agcgtcagtg tggtcttgcc accggattca gggccgtaaa tttcgatgac
     2701 gcgcccgcgc ggcacgccgc ccacacccag agccgcgtcg atcgagagcg cccccgtcgg
     2761 gatcacagtc accggcacca gaacatcgcg gcttccgaga cgcatgatgg cgcccttgcc
     2821 gtaatctttt tcaatctgcg atatcgcagt ttccagcatt tttgatttgt cgtctgtcat
     2881 cacatcacct cgaaggctaa gttgtcacgt tcagtgactg cgctgtccgt tcggagtcta
     2941 ctataacccg ccccacccgg cacaaattca tttcggaagg aaaagatacc atatggcgaa
     3001 taaaaagcaa atactatttt tagagactcg gcgcctttct atctcggggc gggatgtgaa
     3061 ggcctaaaaa ctttaacaca gaggacacgg agaagggcac ggaggagaaa accgttcgag
     3121 aattttccga cattgacatg caagctttct ccggtgtttt ctgttgtcat cctctgtgtc
     3181 ctctgtgtta aagcttttgc gctatttggc ggcgtaggtg taaaacccgc gcccactctt
     3241 cttccctagc cagccggcgg ccaccatttt tctcagtaac gggcaggcgc gatatttgga
     3301 gtcgccgaaa ccttcgtgaa gcacgtcgag aatgtgcacg cacacatcga ggccaatgaa
     3361 gtccgcgagt tcgagcgggc ccatcggatg attcattccc agcttcatga tggagtcgac
     3421 ggattccgcc gtggcgacgc cttccattac tgagaatgcg gcttcattga tcatcggcag
     3481 caacacgcga ttggatacga agccaggcgc gtcgttcacc gcaacgggct ttttctcaag
     3541 ccgctcgcac agcgccatcg tcgtttgaaa tgtttcgtcg ctcgtttgca gcgcgcggat
     3601 cacctcaatg agaaccatca ccggcaccgg gttcatgaag tgcatgccga taaaacgctc
     3661 tgggcgggtg gtggccgcgg cgagcgttgt gacggaaatc gaggaagtat tcgacgccag
     3721 gatgacgtca gggcgcagga gtttgtccgc ttcgcgcagc acggatcgtt tcaggtccaa
     3781 gcgctcgggc acggcttcga cagcaaaatc ggcgcgcgca atttccgaca aatcggttgt
     3841 agtgcgaatg cgcgcaagaa ttttcgattt atcctcttcc gcgatctttc ccttttttgc
     3901 ttcgcggtcc agatttttcg caatcgtatc gcggccgcgc gcgagaaaac tttcgtccac
     3961 gtcgcgcaag atcacttcgt agccgcaacg ggctaatacg tgtgcgatgc cattgcccat
     4021 ggttcccgcg ccgagaaccg cgaccgtgcg aattgcgtca agagtcattt catctccgga
     4081 gaagcaggat ggtgaggaag tataggaagt taaggaggga aaggaaagtg gcgtgggctt
     4141 cagagtctgt gtgagaacaa caaaattgaa gcttcagcgg ctaaagaggc tgcggaaaaa
     4201 atcaacattt tgtcattccg agcgaagcga ggaatccctc cttctttatt ttcttatact
     4261 taaatcgaag agagattcct cgcttcgctc ggaatgacag aataaactac tttttccgca
     4321 aac
//
LOCUS       DASZMB010000223         2523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3764378, whole genome shotgun sequence.
ACCESSION   DASZMB010000223 DASZMB010000000
VERSION     DASZMB010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3764378"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..2119
                     /gene="thrS"
                     /locus_tag="VG272_10460"
     CDS             77..2119
                     /gene="thrS"
                     /locus_tag="VG272_10460"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HWF14147.1"
                     /translation="MMDMIHITMPDGAIREVPLGTTAAEIAQQISPRLAKEALVARIA
                     PIHGPGNPSLSATASLEKSPDAAPEARLTAANHSEGFLVDLRAPIDQDVRLSILTPKD
                     PEAIQVLRHSAAHLLAAAVLELFPNVKLGIGPPIDTGFFYDFVRSEPFTPEDLERIEK
                     KMRELAGQDIPNERKMMPKPEAIELYRKWDQGFKCELVEERAIEPMVSFYTTGKFIDF
                     CRGPHIPSTGRIRAFKLMSVAGAYWKGQEGNPQMQRIYGACFYTQQELDEYLHRLEEA
                     KRRDHRRLGQELDLFSVQEAAGPGLIFWHPKGGLIRKVMEDWLRDELLRRGYDLVITP
                     HIMRLELWKISGHANFYRENMFGPVEVEKDEYQLKPMNCPGHILIYKSRLRSYRELPV
                     RLAELGTVYRYERSGVLHGLLRVRGFTQDDAHIFCMPEQIESEIEACIDFAFAVMKTF
                     GFTQYVVELSDWDANHPENYAGSAEDWARSTGALETTLTRLNIPFKRMEGEAAFYGPK
                     IDVKLIDAIGRPWQLTTVQFDFNLPGRFGLEYVGEDGNRHQPLMVHRALWGSVERFFG
                     ILIEHYAGAFPVWLAPVQAEVLPLSEKFTDYAKTVVAALKGAGFRAHLDDRNEKLQAK
                     IRDAQLLKIPYMLIVGGKEAEAGTVSVRQQSKGDLGPRPLADFLADLRQQVDSRTI"
BASE COUNT          553 a          697 c          699 g          574 t
ORIGIN      
        1 tctgctagcg taggtgattc catgggcgcc tgggcgcagc ttcatttcgc actcgccggt
       61 tgaaagccta cataaaatga tggacatgat tcacattacg atgccggatg gcgctatccg
      121 cgaagtgccg ctgggcacaa cagctgcgga aatcgcgcaa cagatttcgc cacgcctcgc
      181 aaaagaagcc ctggttgcgc gaatcgcccc tattcatggg ccgggcaacc cgtctttatc
      241 cgcgaccgcg tctctcgaaa aatctccaga tgccgctccc gaagcgcgcc tgactgccgc
      301 taatcatagc gaaggttttc tggtcgattt gcgcgctccg atcgatcagg atgtgcggct
      361 ctcgattctg acgccaaaag atccggaagc gattcaagtg ttgcgccact cggcggcgca
      421 tttgctcgcc gctgccgtgc tcgaactttt tccaaacgtg aaacttggca tcggaccacc
      481 gatcgacacg ggatttttct acgatttcgt acgttccgag ccctttacgc cggaagacct
      541 cgaacgtatt gaaaagaaaa tgcgcgagct tgccggacaa gatattccca acgagcgcaa
      601 gatgatgccg aagcccgagg ccatcgagct gtatcgcaaa tgggatcagg gctttaaatg
      661 tgaactggtg gaggaacgcg ccatcgaacc gatggtttca ttctacacga cgggcaaatt
      721 tatcgacttc tgccgcggtc cgcacattcc atcgaccgga cgaattcgcg cgttcaagct
      781 gatgagcgtc gcgggcgcct actggaaagg tcaggaaggc aatccgcaga tgcagcgcat
      841 ctacggcgcg tgcttctata cgcagcagga actggatgag tacctccacc gcctcgaaga
      901 agccaagcgc cgcgatcatc gccgccttgg gcaagagctg gatctattct ccgttcagga
      961 agctgccggg ccgggcctca ttttctggca cccgaagggc ggcctgattc gcaaagtgat
     1021 ggaagactgg ctgcgcgatg aattgctgcg ccgtgggtat gatctggtga tcaccccgca
     1081 catcatgcgt ctcgaactat ggaagatcag cggccacgca aatttttacc gcgaaaatat
     1141 gtttggcccg gtggaagtcg aaaaagacga ataccaattg aaacccatga attgcccggg
     1201 ccacatcctg atttacaaat cgcgcctgcg cagttatcgc gaactccccg tgcgtctcgc
     1261 cgagcttggc acggtgtatc gctacgagcg ctcaggcgtg ttgcatggtc tgttgcgtgt
     1321 gcgcggcttt acgcaggatg acgcgcacat tttctgcatg ccggagcaaa tcgaatccga
     1381 aattgaggcg tgtatcgatt ttgctttcgc cgtgatgaag acgtttggct tcacccaata
     1441 cgtcgtcgag ctttcggact gggacgcgaa tcatccagaa aattatgctg gctccgccga
     1501 agactgggcc cggtcaaccg gagcgctgga aaccacgctg actcgcctga acattccgtt
     1561 taagcgcatg gaaggcgagg cggcgtttta tggaccgaaa attgacgtga agttgatcga
     1621 cgccattggc cgtccgtggc agctgaccac ggtgcaattc gatttcaatt tgcccgggcg
     1681 atttggtctg gaatacgtgg gcgaagacgg caaccgccac cagcctctta tggtgcatcg
     1741 cgcgctgtgg ggctcggtcg agcgcttctt cgggatcctg atcgagcact acgcaggagc
     1801 atttcctgtc tggctcgcgc ccgtgcaggc cgaagttctt ccgctcagcg agaaattcac
     1861 ggattatgcg aaaacagttg tcgccgcatt gaagggtgcc ggattccgcg cgcacctcga
     1921 tgaccgcaac gaaaaattgc aggctaaaat tcgcgacgca caactcctga agattcccta
     1981 catgctgatc gtcggcggca aggaagctga agctggtacg gtctcggtgc gccagcaaag
     2041 caagggtgat ttgggtccgc gcccgcttgc ggactttctg gcggacctgc ggcagcaagt
     2101 cgattcccgc acgatctaat gcttagataa caaacacagt ttgtcttttc gaatcctgct
     2161 ttttcctcgg atcacgtccg tttattttct taagcagttt attaaacttg actattcctc
     2221 aagaaatggg atatgtgggg aagtataaag ggctggtcta tacctatcgt ccggggtcgg
     2281 ccccgttaat tccgattcat actaggtatg cggtggggat ttttttgctt cctcataaga
     2341 attgagggtc catgagtcat ccaattaccc ggcaaggaac gcctgttcct ccgcctgcgg
     2401 cgatgccgtt tcctctccgc ccggcaaccc ctgcacagac acaaactgaa ttcggaaact
     2461 ggtgggacca catgatcgtg cgactgattt ttgcggtcgc atgcgtcgcg gctggatatc
     2521 att
//
LOCUS       DASZMB010000224         6962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1031127, whole genome shotgun sequence.
ACCESSION   DASZMB010000224 DASZMB010000000
VERSION     DASZMB010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031127"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="VG272_10465"
     CDS             <1..1210
                     /locus_tag="VG272_10465"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HWF14148.1"
                     /translation="AERQRLIFLTRLMLPAQFCFYQGSILSAVQYAKGRFVIPSLAPV
                     VYNVMIVLGGIFLASRYGMTGFAIGVVVGAILGNFLLQVYGAFQAGAKFHPNFNLRHP
                     GFILFLKLSVPIMLALSLVFTDDWVIRWFGSYLQPASITWLSYGKTLMRVPLGVVGQG
                     VGVASFPFLAQLYSEGKMDELNRLLNTTMKWLIVLLVPISALTIAESSPVVYLVFSHT
                     RLRGTDFNATAATLMLFSLGMFAWGAQNILARGFYATRNTMTPAIVGTVLAIANLPVY
                     WLLVRRFQHIGLALASSIGIIAYTVALFVLLNRRTHNPDGTGIALFFAKVCGASTVVA
                     YICYRLQLILEPHFAWHTIPGAFALLVIVTSAGIVMLFLASKLLGIREFDELAVRIRK
                     MLVRNPVPAL"
     gene            complement(1394..1744)
                     /locus_tag="VG272_10470"
     CDS             complement(1394..1744)
                     /locus_tag="VG272_10470"
                     /inference="COORDINATES: protein motif:HMM:NF042601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility-associated protein"
                     /protein_id="HWF14149.1"
                     /translation="MFSIIGIVVVFGCVVAGYLMEHGNMKVLMQPAELIIILGAAIGT
                     VLIANPLHILKKIAGGFGKAFGGSPFNKQRYTDDAIEKHGVRGAAARVLQKPFTHDML
                     ARSVREALGPSALK"
     gene            complement(1778..2689)
                     /locus_tag="VG272_10475"
     CDS             complement(1778..2689)
                     /locus_tag="VG272_10475"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWF14150.1"
                     /translation="MTELSDPAIYQTVLDALETGVYIVDPNRRIRFWNEGAEKITGYL
                     RQDVVGHFLRDHLLTASDGSGDLDTDAYDPINIAVRDGKSSLMSVSILHKEGYRLPIT
                     LRTNPIRNSRGMVIGAAESFDKVQPFLDSTRRHSGLADSGCLDVLTGVAAQGFIETKI
                     RENLITFAEHHIPFAVLLMEIDDLEQHRIKRGPGVVSTILRIIAQSLENCLRPTDILG
                     CWGKYQFLAILTDCKESEVPLVAERARRIIGRSEIEWWGDKFAVTSPVGGAGCRVGDN
                     VESLLARADASLHESIAKGGNRVTVLA"
     gene            complement(2734..3459)
                     /locus_tag="VG272_10480"
     CDS             complement(2734..3459)
                     /locus_tag="VG272_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWF14151.1"
                     /translation="MEKHQRKILVIDVGGTHIKVQATGHTKEIKIDSGPKMTAQRMVK
                     EVRDATKDWAYDVISIGYPGPVIHGHPLSEPHNLGGGWVGFDFRKAFGKPVRITNDAA
                     MQALGVYHGGRMLFLGLGTGLGSAMMVDGVLEGMEIAHLPYKKGRTYEDYVGLRGLKR
                     SGKKKWRRRVAVIAGELKTALEADYVVLGGGNAKFLKKLPGGCQLGNNATAFTGGYRL
                     WDDPDEKSKWIKEHHSLEAAKSN"
     gene            complement(3506..5332)
                     /locus_tag="VG272_10485"
     CDS             complement(3506..5332)
                     /locus_tag="VG272_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HWF14152.1"
                     /translation="MPPIIAAQGLSKRYGAEPLFQDISFTVSEGERIGLIGPNGSGKS
                     TLLAMLQGSVAPDTGDVAIRKRVRLCSVAQNSEFPEGATIRSVVADALDRAEVAEKDR
                     AARIAEPLGRAGFTDMDALTASLSGGWRKRLAIVEGLVQAPDVLLLDEPTNHLDLAGI
                     QWLESLLQNATFACVVVSHDRYFLENIATAVVELARSYENGYLRVNGNYSTFLEAKEV
                     YLHAQRKRQEALENLVHTEIEWLRRGPKARTTKSKARIDTAHELIGELSDLNSRTKTV
                     SAKIDFSATDRKTKRLVMLDDVTYEIGGRTLFSGINFAVTSGMRVGLVGPNGSGKTTL
                     LRLLRGDLETTRGEVRRADALRTVYFDQNRELDPDVSLRRALAPDSDSVIYQDRVIHV
                     AAWATKFLFDPEHLSQPVGKLSGGERARVLIAQLMLQAADVLLLDEPTNDLDIPTLEI
                     LEESLLEYRGALVLVTHDRYMLDRVSTIVLGLDGLGGAERFADYSQWEIWQRAQQKGT
                     TNLSTGTKRSGAQGEAPPASSKKRLSYHEVRELETIEERIAAADKNLEAKHVALEDPG
                     VTSDPERLKTACIEMEEAQSTLDHLYERWGQLEKKKAGGQAS"
     gene            complement(5696..6142)
                     /locus_tag="VG272_10490"
     CDS             complement(5696..6142)
                     /locus_tag="VG272_10490"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HWF14153.1"
                     /translation="MRIKATSKILPLLFLCLLGPLCGRVAAQTPPDSGKGYAKLIYSV
                     KGPDLFRAHCAACHGAGGKGDGPAASALKTKPADLTALAKNNSGKFPVERVRKFISGD
                     DPSLSSHGSREMPVWGPIFHQIENDQDLGNVRLQNLIAYLETIQQK"
     gene            complement(6461..>6962)
                     /locus_tag="VG272_10495"
     CDS             complement(6461..>6962)
                     /locus_tag="VG272_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14154.1"
                     /translation="PVQRFCDPAGIPRIYFYPWLINIIQTPKTVYILYEYSKVWRAIA
                     MNAEHPKDPDSTWMGDAVGKYEGDTLVVDTIGFNDKTWLDQAGHPHSDALHVIERFRR
                     RNQGKLELNVTIDDPKAYTRTFSATKMYNHSDGPVGETLCAYSEMKEFQENVVDKTQT
                     PPAKPR"
BASE COUNT         1436 a         1995 c         1858 g         1673 t
ORIGIN      
        1 ggcggagcgg cagcgactca tttttctcac gcggctcatg ctccccgcgc aattttgctt
       61 ttaccaggga agtattctca gcgccgtgca atatgcgaaa ggacgtttcg taattccctc
      121 tctcgcgccg gtcgtctaca acgtgatgat tgttctcggc gggatttttt tggcgtcgcg
      181 ctatgggatg acgggcttcg cgatcggcgt agtagtcgga gcgattctgg gaaatttcct
      241 gctgcaggtt tacggcgcgt ttcaggcggg ggcaaaattt catcccaatt tcaacctgcg
      301 gcatcccgga tttattttat ttctgaagct ttcagttccc atcatgctgg ccctatcgct
      361 ggtgtttacg gacgattggg tgatccgctg gttcggctcg tatttacagc ctgcatcgat
      421 cacgtggctc agctacggga aaacgttaat gcgcgtgcca ctcggcgtcg tcggacaggg
      481 ggtgggagtg gcttcatttc ccttcctcgc tcaactctat tccgaaggaa aaatggatga
      541 gctgaatcga ttgttgaaca cgacgatgaa gtggctgatc gttttgctcg ttccgatttc
      601 ggcgctcacg attgcggaaa gctcgcccgt ggtttacctg gtgttctccc acacgcgatt
      661 gcgcgggacg gattttaatg ccacggccgc cacgctcatg ctgttttctt tgggcatgtt
      721 tgcgtggggg gcgcaaaaca ttctggcgcg agggttctac gccactcgca atacgatgac
      781 gccggcaatt gtgggtacgg tgctcgctat tgccaatctg cccgtctact ggttgctcgt
      841 gcgccgcttt cagcatattg gattggcgct ggccagttcg atcgggatta ttgcctacac
      901 cgtggcgctt ttcgtgctgc tgaatcgacg cacgcacaat ccggacggca cgggcatcgc
      961 cttatttttc gctaaggtat gcggggcttc gacggtcgtc gcctatatct gctaccggct
     1021 gcaactgatt ctcgagccgc atttcgcgtg gcacaccatc ccaggagcgt ttgcattgct
     1081 ggtgattgtc acgtcggctg gaattgtgat gttgtttctt gcttcaaaat tgctgggaat
     1141 tagggaattc gatgagctgg cggtgcgcat tcggaaaatg cttgttcgca atccggttcc
     1201 tgcattgtga ctgctgcgtc taaacgtatc gagagcgagt ctttgagtag cacaggcact
     1261 cttgcctgtg tggattttgc gaaatgcaca caggcgagag tccctgtgct actcgaccat
     1321 tgatcgtcat tccgaacgcg aggacgaatt tcttttggat tttaagtgta agaagacagg
     1381 ctgaaagcct gtcctacttt agggcggacg ggcccagcgc ttcgcgaacg ctgcgggcga
     1441 gcatgtcgtg cgtgaagggc ttctgcaaga ctcgggcggc tgcgccgcga acaccatgct
     1501 tttcgatggc atcgtcggta tatcgctgct tgttaaatgg cgatccccca aacgcttttc
     1561 caaagccgcc cgcgattttt tttaagatat gcaggggatt ggcgatcaag acggttccga
     1621 ttgcggcgcc caggatgatg atcaattctg cgggctgcat caaaaccttc atgttgccgt
     1681 gttccatcaa atagccggcg accacgcagc caaagaccac cacgattcca ataattgaaa
     1741 acatcgatgt gcaatcctcc gaagtaattc aagggcgtca ggccaaaacg gtgacgcgat
     1801 ttccaccttt ggcaatgctt tcgtgaagtg acgcgtcggc gcgcgccagc aaactttcga
     1861 cgttgtctcc cacccggcag cccgctccgc ccaccgggct tgtgaccgca aatttgtcgc
     1921 cccaccattc aatttcagag cggccgatga ttcgcctggc ccgctccgca accagcggca
     1981 cttccgattc cttgcaatcc gtgagaatcg ccagaaattg atattttccc caacacccca
     2041 aaatatccgt gggccgaaga caattttcta gagactgcgc gatgattcgt aaaattgtgg
     2101 atactacgcc cggcccgcgc ttgatccgat gttgttcgag gtcatcgatt tccatgagca
     2161 gcacggcgaa cggtatatgg tgttcggcaa aagtgatgag gttttcgcgg attttcgtct
     2221 caataaatcc ctgcgcagcg acgcccgtta gaacgtcgag gcagccggaa tccgcaaggc
     2281 cggagtgacg ccgcgtggaa tccagaaacg gctggacctt gtcaaaactt tccgcggctc
     2341 cgatcaccat gccgcgactg ttccgaatcg gattcgtacg aagtgtgatg ggcagccggt
     2401 atccttcctt gtgcaggatg gaaacgctca tgagggaaga ttttccgtcg cgaacggcaa
     2461 tattgatggg gtcgtaggcg tcggtgtcca ggtctccgct cccgtcgctt gcggttaaca
     2521 aatggtcgcg caggaaatgg ccaaccacat cctggcgcag atacccagta atcttttccg
     2581 cgccttcatt ccagaatcgg atgcgccggt ttgggtcaac aatatagacg ccggtttcga
     2641 gcgcgtctaa tacggtttgg tagatcgctg gatcacttaa ttcggtcatg agatgactcc
     2701 acgtggaatc atcgtccaat aagggaaagg agtttagttc gacttagcgg cttctaagga
     2761 gtggtgttcc ttgatccatt tggatttttc gtccggatcg tcccacaggc ggtaaccgcc
     2821 ggtgaatgcg gtcgcgttgt tgcccagctg gcatcctcca ggcaacttct tcaaaaactt
     2881 cgcattgccg ccgccaagaa ccacataatc cgcttccaac gcggttttta gctcgcccgc
     2941 gatcacagca acgcggcgac gccatttctt cttcccggag cgtttcaatc cgcgtaatcc
     3001 cacgtagtct tcgtacgtgc gtcctttttt gtacggcaaa tgcgcaattt ccatgccctc
     3061 taagactccg tccaccatca ttgctgagcc aaggcccgta cccagtccga gaaaaagcat
     3121 ccttccgccg tggtaaacgc ccagagcctg cattgcagcg tcattggtaa tccgcaccgg
     3181 cttcccaaac gctttgcgaa aatcaaaacc cacccaccca ccgccgagat tgtgtggctc
     3241 gctcagcgga tgcccgtgaa tcaccggacc cggatatccg atggagataa cgtcgtaggc
     3301 ccagtccttt gtagcatcgc gcacttcctt gaccatgcgt tgggcagtca ttttcggtcc
     3361 ggaatcgatt ttgatctctt tcgtatgccc cgttgcctgc actttaatgt gcgtaccgcc
     3421 tacgtcaatc accagaattt ttcgctgatg cttttccatc tccactcctc tttctttatc
     3481 ccaaagtcca cgggccgagg cacctctaag aagcttggcc gcccgctttc tttttttcca
     3541 gctggcccca acgctcatag agatggtcca acgtgctctg ggcctcctcc atctcgatgc
     3601 acgcggtttt cagacgctcc ggatcgcttg tcaccccggg atcttccagc gcgacgtgct
     3661 tggcttccag atttttgtcc gcggcagcga tgcgctcttc gatggtctcc aactcgcgca
     3721 cttcgtgata ggaaagtctc tttttggaac ttgcaggcgg agcttctccc tgtgcgccgg
     3781 aacgcttcgt tcccgtggaa aggttcgtcg tgcccttctg ctgcgcgcgc tgccagatct
     3841 cccactgcga gtaatcggca aaacgctcgg cgcccccgag tccgtcgagc cccagaacga
     3901 tcgtcgatac gcggtcaagc atgtagcggt catgcgtaac gagcacgagc gcgccgcggt
     3961 attcgagcaa gctttcttcg agaatttcca gcgtgggaat gtccagatcg ttggtcggct
     4021 cgtcgagcag taaaacgtct gccgcttgca gcatgagttg cgcgatcagc acgcgcgccc
     4081 gctcgccgcc cgaaagtttt cccaccggtt ggctgaggtg ttcaggatcg aaaagaaatt
     4141 ttgtcgccca ggcggccacg tgaatcacgc gatcctggta aatcacggaa tcgctatccg
     4201 gcgcaagcgc gcgccgcagg ctgacgtccg gatcgagttc ccgattctga tcgaagtaca
     4261 cagtgcgcaa tgcgtcggcg cgacgcacct ctccccgggt ggtctcaaga tcgccgcgca
     4321 gcaagcgcag tagagtggtt ttcccgctcc cgttcggccc gaccaggcct acgcgcattc
     4381 cggaagtcac ggcaaaattg atcccggaaa ataaagtacg gccgccaatt tcgtaggtca
     4441 catcgtccag catcaccagc cgcttcgtct tacggtccgt agccgaaaaa tcgattttgg
     4501 cggagaccgt cttcgtgcgg gagttcaggt ctgacaattc gccgattaat tcgtgtgccg
     4561 tatcgatgcg tgcctttgat tttgtggtcc tggctttcgg cccgcgccgc agccattcga
     4621 tttcggtgtg cactaaattt tccagcgctt cctgccgctt gcgctgcgcg tgcaggtaca
     4681 cttctttcgc ttccagaaac gtgctgtagt tcccgttcac ccgcaggtaa ccgttttcgt
     4741 aggaacgcgc gagttccaca acggcggtcg cgatattttc cagaaagtag cggtcgtggc
     4801 tgaccacaac acaggcgaac gttgcattct gcagtagcga ttcgagccac tgaatgcccg
     4861 cgagatccag gtggttcgtc ggctcgtcca gcagcaaaac gtcaggcgcc tgcacgagac
     4921 cctcgacaat tgccagtctc ttgcgccagc cgcctgacaa cgacgccgta agcgcatcca
     4981 tatcggtgaa acctgcgcgc cccaaaggtt cggcgatgcg cgccgcgcga tctttttccg
     5041 caacttcggc gcgatcgagc gcgtcggcga ccacggaacg aatcgtcgcc ccttccggaa
     5101 attcggaatt ctgcgcgacc gagcacaaac gcacgcgctt gcggatcgcc acatcgcccg
     5161 tgtcaggcgc cacgctgcct tgcaacatcg cgagaagcgt ggatttgcct gatccgttgg
     5221 ggccgatcag gccaattcgt tcgccctcag aaaccgtaaa ggagatgtcc tgaaaaaggg
     5281 gttcggcgcc gtatctcttg gaaagtcctt gtgcagcaat aatcggaggc aaatgactaa
     5341 gttttctccc gcaagcagtg tagcgtgaac gcgcaacatc cgcaaaacat tggggaatta
     5401 agtggcgcac gcctgagttt gcgggtttgg tgctagtgag gaccaaggcc gcgccactaa
     5461 aaccaatcgc tcctgtaggc cacccaccag ctcacccacg cgagcggaat agtcgcgccc
     5521 agagcccacc aaaacggcag agcaagatcg gcaataatcc cgagcacact gaatagcccc
     5581 caaaatactt tacgttgcat aagtctattg taaaccggat gcagagattg aaaccaagcc
     5641 tgcgcgagaa gtgcaactgg agacgggacc cgaaaaaaag aaaactctgg tagcgctatt
     5701 tctgctgaat ggtttccagg tacgcaatca aattttgcag acgcacgttg cccagatcct
     5761 gatcattctc gatttggtgg aaaatcgggc cccagacggg catttcccgc gagccgtgag
     5821 aactcaggga cggatcatcg cccgaaataa atttccgcac gcgctcaact ggaaactttc
     5881 cgctgttatt ttttgcgagg gccgtcaagt cggcgggctt ggtttttagc gccgacgcgg
     5941 ccgggccatc gcccttgccg ccggctccat ggcacgcggc acagtgagca cgaaagaggt
     6001 cgggcccctt cacggaataa attagtttcg cgtagccctt gcctgaatcc gggggagttt
     6061 gggcagccac cctgccacat agagggccga gcaaacacag aaaaagaagg ggcagaattt
     6121 tcgatgttgc tttgatcctc attctgcccc cctttggtac ctggtgttat tggcacttac
     6181 atcagtgaag ctttcaaccc tcgcggttca tacgtggaac cgataaaacc agacgagttt
     6241 tatagtcact taaatcccgc ctctttttag tgccgaaggt tgtgcgtgcc tccccaatgc
     6301 ataaaaaaga tcaagcccat catgagcaac gccatgaaca tatagtcttg tctgaccatg
     6361 acgccctcct tttgctttca atccttacag actgaagttt gctcttccga accaagaatt
     6421 gctgtgacgc gagaaaccct tgcaggtgac gtacgtcacc tcaccgcggc ttcgcgggcg
     6481 gcgtttgtgt cttgtccacg acgttctcct gaaactcttt catttcggaa taggcgcaga
     6541 gagtttcccc gactggcccg tcggaatggt tgtacatttt cgtcgcggag aacgttcttg
     6601 tgtacgcctt gggatcgtcg atcgtgacgt tcagctcaag cttgccctga ttccggcggc
     6661 gaaatcgctc gatcacgtgc agcgcgtcgc tgtgcgggtg ccccgcctga tcgagccatg
     6721 ttttgtcgtt aaaaccaatc gtgtctacaa ccagggtgtc accttcatac ttccctaccg
     6781 cgtcacccat ccaggtgctg tctggatcct taggatgctc ggcgttcatg gcgatggccc
     6841 gccacacttt cgagtattca tacaggatgt acaccgtctt cggagtttgg atgatattga
     6901 tgagccaagg ataaaagtaa attcgcggaa ttcccgccgg atcgcagaat ctctgcaccg
     6961 gg
//
LOCUS       DASZMB010000225         6654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3766043, whole genome shotgun sequence.
ACCESSION   DASZMB010000225 DASZMB010000000
VERSION     DASZMB010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6654
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3766043"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VG272_10500"
     CDS             complement(<1..332)
                     /locus_tag="VG272_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14155.1"
                     /translation="MELAADLRRALDGLCAIGAPEVRENGEKLAGLAALEYEVSAQGD
                     AALLHLWSTQQSLVRRVLRVAEQSDDRVVLEVSRLGFSRNAQLEFVATAAPREPHRIE
                     REQFSTRFR"
     gene            complement(865..1338)
                     /locus_tag="VG272_10505"
     CDS             complement(865..1338)
                     /locus_tag="VG272_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14156.1"
                     /translation="MPTEERSRLPVAFLIGIAIVAVLVSGAVLYSRYAAPAISEDDKP
                     VSFSPEAKAYAPQIHFLEPKMSRAANFLNQEVTYVFCTVENGGDKKIHQIEITVEFHD
                     PFHQVILRDKQRLFLSTAPPFLPGQQRDVQIPYEHIPPQWDQVFPSITVTALEFK"
     gene            complement(1501..2244)
                     /locus_tag="VG272_10510"
     CDS             complement(1501..2244)
                     /locus_tag="VG272_10510"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HWF14157.1"
                     /translation="MRYLIISDIHANLTALDACLAAVNGRWDRAFCLGDLVDYGPDPN
                     EVTEFIRELSPVIIRGNHDKAVAGITDLEDFNPMARVAAQWTRTQMHPVNLEYITKLP
                     MGPLSPDGITMVHGSYEDEDEYVFVPGQAMGGLMESPTDVTFFGHTHYQGGFSYRAGQ
                     IGMIQLRPDHGANFGALRMEPGTRYLLNPGSIGQPRDGDPRAAFAIADLEHHIVEFWR
                     VPYDIAAVQKRMEKAGLPFPLVERLGAGR"
     gene            2556..4052
                     /locus_tag="VG272_10515"
     CDS             2556..4052
                     /locus_tag="VG272_10515"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HWF14158.1"
                     /translation="MRNRNRNNTPSAKADANLSRRTLLQRAGWTAAALAVPSGMFAAD
                     AGISPVMAKLSAYMAEAGGRALPDEVIEKAKHHILDTFAAMISGSDLPPARAAFNYAR
                     DFNTDKTSTIVASKMTSSPPEAAFINGMLAHSDETDDSNEFSQSHPGCAVVPATLAAG
                     EKFGIDGTRFLRAVTLGYDVGPRMTISFGALDFRNHSHKSTHAISSVFGAASAAGCAA
                     DLNAQQMRWLLDYTSQQSSGIGAWNRDTEHIEKAFVFAGMNARNGVTSALVVHAGFDG
                     LDDIFSGVDNYFDAYAPTANKEELVSQLGERYEIARTNIKKWTVGSPIQAPLDCLYNI
                     LKKHPINPDDVKTIVVREANSEARVVDNREMPDICLQHMVAIMILDKTASFKAAHDKP
                     RMKDPAVLAVRAKVQLVPDAELEKMEPARQAIVEITMNDGTKYSDHVADVRGTVDNPM
                     TREEVVQKCRDLVEPVLGKANCTTLIDKILGLEKVKDIRELRSVLQKG"
     gene            complement(4083..5414)
                     /locus_tag="VG272_10520"
     CDS             complement(4083..5414)
                     /locus_tag="VG272_10520"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HWF14159.1"
                     /translation="MAEPEITVLNPDADGPGRRSSFQTLWDRLKEHPLARVGPGLITG
                     VADDDPSGIATYSQAGAEFGLNMLWTMPLAYPLMAALQSMCGDIGRVTGKGLAANIKC
                     TFSPVVLRTVVLLLLVANTLNIAADIAAIGEVGQLMTGVNRHVMTLLFVVGTLLLQIL
                     IPYHRYVKYLKWLTLSLLAYAAVLFTVHVPWREVALRTVWPRFTPNSAAAAVIVGVFG
                     TTISPYLFFWQASEEVEDMQDPPVVPPLVSDAHEAKKELCRIRWDTWTGMFYSNLSAF
                     FIILATALTLHVAGITSVDTAAQAASALRPLAGNFAYALFALGILGVGLIGVPVLAGS
                     AGYALCEAMGWKWGLGRKATDARGFYGVITVSVLAGLVIQYSPISPMKALFWSAIING
                     VVAVPLMIVIILLVSKCAVMGDYTASRPILILAWIATLVMGLAAVRMLIPG"
     gene            complement(5513..6184)
                     /locus_tag="VG272_10525"
     CDS             complement(5513..6184)
                     /locus_tag="VG272_10525"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1572 family protein"
                     /protein_id="HWF14160.1"
                     /translation="MLARVARIMEHLGQIQWKYSDRPAASAVTLSLRHAASSYEGPIQ
                     IENMTNSRLQHVGLPADFLTFSREKLLEQYWPRLRSCVESLTDEQVWWRPNDASNSIG
                     NLILHLNGNVQQWIISSFNHLEDTRDRPAEFSERRMIPAAALLEKLGATMQEASEVLA
                     RLAEADLQTSFQIQGYMVTGLRAVYQVVEHFGIHYGQIVFITKLVRAKDLGFYRELDK
                     TGRLP"
     gene            complement(6194..>6654)
                     /locus_tag="VG272_10530"
     CDS             complement(6194..>6654)
                     /locus_tag="VG272_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWF14161.1"
                     /translation="AVENFEQADALLFGRVTYEMMEAAWRPPAEVVGRPDWMEPFARA
                     IEAAKKYVVSSTLERVDWNAELVRGDLGKAVQQLKREPGKGLFVGGVKLPLALAELGL
                     IDEYEFVVHPRLAGHGPTLFAGLSKHVDLRLVSRLEFGSGAVAMRYEPRR"
BASE COUNT         1565 a         1890 c         1813 g         1386 t
ORIGIN      
        1 cgaaaccggg ttgaaaactg ttcgcgttcg atgcggtgtg gttcgcgcgg cgctgcggtg
       61 gcgacgaatt cgagttgtgc gtttcttgaa aatccaagac ggctgacttc gagaacaacg
      121 cggtcgtcag attgttcggc gacgcgcaag actcggcgaa ccagggattg ctgggtactc
      181 cacaaatgca gcaaggcagc gtcgccctgc gcgctgactt catattccaa cgcggccagc
      241 ccggccagct tttccccatt ttcgcgcact tcaggcgcgc cgatggcaca tagtccatcg
      301 agtgcccgac gtaaatcggc ggccaactcc attggcacgc attattgaat agaagacgga
      361 aaaggagaag tcatttttcg tgacaaatgc gcggaaattg agatgtgcga ttagaagcaa
      421 tttcataaat gaccaggtac tccagctctt taggctgcca gaggctaaag agtccgtgtg
      481 agaactgcaa gattcgaact tcagcggcta aagccgaacc tgattttgct ggacttatgt
      541 cgtggctaaa gccacgaccc acaaagattc cgcagtcctc acacagactc taaagcccag
      601 ttttcttgca ttattgatgt cgcggctaga agccgcgacc cacaaatgta tatatgagat
      661 ggcttggctc ggttgacatc ctggatactt cagaaagttt cgcccgtttt gtggagccgg
      721 aattgtagct cgcctcttca gaggcgaggg tttagatgct ggagcgccaa aacacaagcc
      781 tttgaccctt cgcgttctct cagggcaaga gaggcgggct acagcagatc ggcggaacgg
      841 ggatgtcaac agagccagat ggccttattt aaattcaagg gcggttacag tgatggacgg
      901 aaaaacctgg tcccactgcg gtggaatgtg ttcgtaggga atctgaacgt ccctttgctg
      961 gccgggaaga aatggcggag cggtcgaaag aaacagtcgc tgcttatcgc ggagaatcac
     1021 ctggtggaaa ggatcatgga actcaaccgt gatttcgatc tggtgaattt ttttgtcgcc
     1081 gccattttcc accgtgcaga aaacataggt aacttcctgg ttcaggaaat tcgcagcgcg
     1141 gctcatcttc ggttcgagaa aatgaatttg gggtgcgtac gcttttgcct cggggctaaa
     1201 ggataccggc ttatcatctt ccgaaatggc aggtgcggca taacggctgt agagaacagc
     1261 tccgctgaca agcacggcga cgatagcaat tccgatgaga aatgcgaccg gtaaacggct
     1321 gcgttcttct gtgggcatca aatcggaaat cctcctgttt ctctttcgct ccgcttgcgt
     1381 cgcctgaaat gatgcagagg cttgttgagc gcgtgacttt gtagcgcccg ccttcaggcg
     1441 ggtacggagc gctccacccc tgcccgccta aaggcgggtg ctacatgaac caagctcgat
     1501 ttaccggccc gcgccaagtc gctcgaccag cggaaaaggc agtccggcct tttccatgcg
     1561 cttctgcaca gcagctatat catacggcac gcgccaaaat tcaacgatgt gatgctccaa
     1621 gtccgcgatg gcaaacgcgg ctcgcggatc tccatcccgt ggctggccga tggagccagg
     1681 attcagcaaa tagcgcgtgc caggctccat tcgcagcgcc ccaaaattcg ctccgtgatc
     1741 gggacgaagt tggatcatgc caatttgccc tgcacgataa gaaaatccac cttgatagtg
     1801 cgtgtgccca aaaaaagtga cgtccgtggg cgattccatc agcccgccca tggcttgtcc
     1861 ggggacgaaa acgtattcat cttcatcttc gtacgagcca tgcaccatgg tgatgccgtc
     1921 cggagagagc ggtcccatgg gtaatttcgt aatgtattct aaatttaccg gatgcatttg
     1981 cgtgcgcgtc cactgtgccg cgactcgcgc cattggatta aaatcttcaa gatcagtaat
     2041 tccggcgacc gctttatcgt ggttgcctcg gatgatgacg ggcgaaagct cccttatgaa
     2101 ctcggttact tcattcggat caggcccgta atccacaaga tcgcccaggc agaaagcacg
     2161 atcccatcga ccgtttacag cggcgagaca cgcatcgagc gcggtgagat tcgcgtgaat
     2221 atcgctaatg atgagatatc gcattgttgg tgaaaaatcc gatccactgc ctcggcttgc
     2281 accgattata gacgaaattc aaaatcacgc atggctagct tggaaatccc tccgtgagca
     2341 gttagagaaa aaaatcccac cgaagagatg aacgattgat gatcctattc atttggattg
     2401 cttgcaaacc aatttttgca atgcatgcgg atcgcttgaa aatttctata cagattagct
     2461 caatcccacg cccgcgcctg cgcagtaaaa aagtctgccg acaaatcttc cgtcagccgc
     2521 cgctgccatg ttaggatttt gcgggagacc caaccatgcg aaatcgtaat agaaacaaca
     2581 cgccttcagc gaaggctgat gcaaatttga gccgtcgaac gctgctgcag cgcgctggat
     2641 ggaccgctgc cgcgctggcc gtgcccagcg gaatgtttgc cgcagacgcg ggaatcagcc
     2701 ccgtgatggc caagctcagc gcctacatgg ccgaagcggg tggccgcgcg ctccccgatg
     2761 aagtgatcga gaaggccaag caccatattc ttgacacgtt tgccgccatg atttcgggat
     2821 cggacttgcc tccggcgcgc gccgcgttca actacgcgcg cgactttaat acggacaaga
     2881 cttcgacgat cgtcgcatcg aaaatgacgt ccagcccgcc cgaagctgcg ttcatcaacg
     2941 gcatgctggc gcattccgat gagacggatg actccaatga attttcgcaa tcgcatcctg
     3001 gctgcgcggt tgtccccgcg acgctcgcag cgggagaaaa atttggaatc gacgggacgc
     3061 gatttctgcg cgccgtgacg ctgggctatg acgtcggccc ccgcatgacc atcagctttg
     3121 gcgcgctcga tttccgcaac cacagccaca agagcacgca cgcgatttcc agtgtgttcg
     3181 gcgcggcgtc cgcggcggga tgcgcggcgg atttgaacgc gcaacaaatg cgctggctcc
     3241 tcgactacac ttcgcagcaa tcctcgggca tcggcgcgtg gaatcgtgat accgagcata
     3301 tcgaaaaagc ttttgtgttt gccggaatga atgcgcgcaa cggggtcaca tcagccctcg
     3361 tcgtgcatgc cggctttgac ggcctcgatg atattttttc gggcgtggac aattacttcg
     3421 atgcctatgc gcccactgcg aataaagagg aactcgtctc gcaacttggc gagcgttacg
     3481 aaattgcgcg caccaacatc aaaaaatgga cggttggttc gccgattcag gcgcccctcg
     3541 attgcctgta caacattttg aaaaaacatc ccatcaatcc cgatgatgtg aaaacaatcg
     3601 tcgtgcgcga ggccaatagc gaagcgcgcg tcgtcgacaa ccgcgagatg cccgacatct
     3661 gtctgcagca catggttgcc ataatgatcc tcgataaaac cgcatcgttc aaagccgcgc
     3721 atgataaacc gcgcatgaag gaccctgctg tcttggccgt tcgcgccaag gtgcagctcg
     3781 tgcccgacgc ggaactcgag aaaatggagc ccgcgcgcca ggccatcgtc gagatcacca
     3841 tgaacgacgg aacgaaatat tccgatcacg tcgccgacgt gcgcggcact gtcgacaacc
     3901 cgatgacccg cgaagaagtc gtccagaaat gccgcgacct cgtcgagccg gtcctcggca
     3961 aagcaaactg cactacgctg atcgataaaa ttcttggcct ggaaaaagtg aaggacatcc
     4021 gggaactgcg cagcgtgctg cagaaaggtt agttaaatat cgctgtgtgc ttgagcaggg
     4081 gcttagccgg gaatgagcat gcgcacggcg gctaagccca tgaccaacgt agcaatccac
     4141 gcaagaatta gaatggggcg gcttgctgtg tagtctccca tcaccgcaca ttttgaaacg
     4201 agcaaaataa ttacgatcat caggggaacg gcgactacgc cgtttatgat cgcgctccag
     4261 aataatgctt tcatcgggct gatcggcgag tattgaatca caagtcctgc gaggacactc
     4321 acggtaatca cgccgtaaaa acctcgcgcg tcggtagcct tgcgccccaa gccccatttc
     4381 cagcccattg cttcgcaaag cgcgtagccg gcggacccgg cgaggactgg cacgccgatc
     4441 agcccgactc ctaaaattcc aagggcaaaa agagcgtagg cgaaattccc ggccagaggc
     4501 cgaagcgcgc tggcggcttg cgctgccgtg tcgacgctgg taattcccgc aacgtgcagc
     4561 gtcagcgcgg tagccagaat aatgaagaat gccgagaggt tggaataaaa catgccggtc
     4621 cacgtgtccc aacggatgcg gcacagttct ttttttgctt catgggcatc ggacacgagc
     4681 ggcgggacaa ccggtggatc ctgcatgtcc tcgacttcct cggacgcctg ccagaaaaag
     4741 agatagggac tgatggtggt gccgaagacg ccgacaatca cggcagccgc cgcggagttg
     4801 ggagtaaatc ttggccacac cgtgcgcagc gctacttcac gccacggcac gtgaaccgtg
     4861 aacagaacag cggcgtatgc cagtagagaa agcgtcagcc acttcaggta cttaacgtaa
     4921 cgatggtagg gaattaaaat ttgcagcagc agcgtgccca ccacaaaaag gagcgtcatg
     4981 acatgacgat tcactccggt catgagttgg ccgacttcgc caatcgcggc tatatcggcg
     5041 gcgatgttca gcgtgttcgc caccagcagc aagagaacga cggtccgcaa cacaaccggc
     5101 gagaatgtgc atttgatgtt tgcggcgaga ccttttcccg tcacccggcc aatgtccccg
     5161 cacatggatt gcagcgcggc catcaaagga taagccagcg gcatcgtcca aagcatgttg
     5221 aggccgaatt ccgcgcccgc ctgggaatac gtggcaatac cacttgggtc atcgtccgca
     5281 acgccggtga tcagccctgg gccgacacga gcgagtggat gttccttcag gcgatcccaa
     5341 agcgtttgga agctactacg gcgcccaggt ccatcggcgt ccggattcag tacggtgatt
     5401 tctggctcag ccatctaaat tccgcatgga atgcgctcag tgtgcaggta taacgaattt
     5461 tccacgataa caccatacag caaatcgtcg tgggggttag gtcaagatga attcagggaa
     5521 ggcggccagt tttatccagt tcgcggtaaa agccaagatc tttcgcgcgc acaagcttgg
     5581 tgataaaaac aatttgtccg tagtgtattc cgaagtgttc cacgacctgg taaacagccc
     5641 ttaaccctgt caccatgtag ccctggattt ggaacgaagt ctgtaaatct gcttccgcga
     5701 gccgcgcgag aacttccgac gcctcttgca tggttgcacc gagcttctcc agcagggccg
     5761 ctgcgggaat catccggcgt tcggaaaatt ccgcgggtct gtcgcgtgta tcttccaaat
     5821 gattgaatga agaaatgatc cactgctgaa catttccatt caaatgcaaa atcaaattcc
     5881 caatgctgtt gctggcgtca ttcggacgcc accacacttg ctcgtccgtc aaggactcga
     5941 cgcagctgcg caaccgtggc caatattgct ccagaagttt ttctcgggaa aaggtcagga
     6001 agtcggcggg caatccaacg tgctgtagac gcgaatttgt catgttctcg atctgaatag
     6061 ggccttcgta agaactagcg gcatgtcgca gagacaacgt taccgcggat gcggcgggtc
     6121 gatcggaata tttccactgg atctgcccga gatgctccat gatgcgggcg actcgggcta
     6181 acaagcccaa tggctatctt ctcggctcat accgcatcgc caccgccccc gagccgaact
     6241 ccagccggct cacgagcctc aagtcgacat gcttcgacag acccgcgaac aacgtcggcc
     6301 cgtggcccgc tagcctgggg tgcaccacga actcgtactc atcgatcaat cccagctccg
     6361 ccaacgccag cgggagcttc acgcctccga cgaacagtcc cttacccggc tcccgcttga
     6421 gttgctgaac ggccttccct aggtccccgc gcacgagctc cgcattccaa tccacccgct
     6481 ccagggtgct cgatacaacg tactttttcg ccgcctcgat cgccctggcg aagggttcca
     6541 tccaatcggg cctccctacc acctccgccg gcggtcgcca cgctgcctcc atcatttcgt
     6601 aggtcacccg gccgaagagg agggcatcgg cctgctcgaa gttctcgacg gcgt
//
LOCUS       DASZMB010000226         3585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_5165138, whole genome shotgun sequence.
ACCESSION   DASZMB010000226 DASZMB010000000
VERSION     DASZMB010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5165138"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VG272_10535"
     CDS             <1..958
                     /locus_tag="VG272_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14162.1"
                     /translation="ENHQRPYSNALMIVDLSDPANVQEVSRWWVPGQKLGEEEEYKKY
                     RFAGDKISWTGNHGALTVPKRVEDGGTIGYGGFGAFGFYAMDLSDIKHPKPYGHVQYE
                     FDTFGTIPFHTCYPLSGDAAHPQLMNKVVAVHEALEADCRENYHTPYILDVKDPKNPK
                     IIGLFPRPAAPPDAPYTDFCFARGRFGSHNTQCWLAPGTSNPAIMVIAWFNAGVRVFD
                     ISNPTAPKEIAWFVPPLEGEINNYESWWRGTTENVFVEFDRNLIWIGTHEGSYCLSCP
                     ALGKAITEPAKVAKWSIPHCNVGWDDQTARSFYFGRSLSQMG"
     gene            1052..2071
                     /locus_tag="VG272_10540"
     CDS             1052..2071
                     /locus_tag="VG272_10540"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HWF14163.1"
                     /translation="MNNRKVSRREFTYGALAAGAVLFTGSRSPAADFELRQFHNQPAD
                     SPLDQWLSKMWAGVKTETNGRVQVQVFADNNKIAGGDPAALNMLVGGEVDFFTLNGGS
                     IGNIVPACNVQGLLCAFRSTQQIFESLDGDLGDYLREEMKSKGIYGFPKGCFDNGMHQ
                     ITCATRPIRTVDDLQGLKIRTPDAPIYTEAWKAMGAAPVVANFNKLYETLKTGAAEAQ
                     TNPLAVAEFLKLYEVQKYVSVTNHGWSGFNLMANLKMWERIPADLQKIIERNVVKFVR
                     QQREELTALNSEFRTKLATQGMTFNDADISSFRPRLVAYYAHWKEVAGDRAWKLLEAR
                     TGKLV"
     gene            complement(2191..3060)
                     /locus_tag="VG272_10545"
     CDS             complement(2191..3060)
                     /locus_tag="VG272_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14164.1"
                     /translation="MGRIGCGVSSRSVLQAIILSAFLLISGVAFSQEAPPLPPESSAP
                     ATLPGQAVLNSAGRCVQPPPMVQMKDYNGPLHKVVGTFTQKLDRQSVHDPHYKQGSML
                     CSLQIKDKFFLFLHDAIDPETIFSAGFNAGISQAENNDPSFGQGAKGYGLRLAVSYTD
                     QVQSRFFKEFAYPTIFREDPRYYRVGQGPANRRFLHAVEHAVVAYNDSGRPMFNFSEW
                     LGEISGASISNLYHPGNGRGVGPAAQNIGIDIALDIGFDELREFWPEAARKLKLPFRD
                     QGEQLNITAPAAK"
     gene            complement(3128..>3585)
                     /locus_tag="VG272_10550"
     CDS             complement(3128..>3585)
                     /locus_tag="VG272_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14165.1"
                     /translation="TFSHSVALSASGLPPGATASFTPASIPAGSGTTGIILSIETAPH
                     SSGSGGRILLAPRDTWMVSISALLVAGALWICLCGRSRKIKMRPLAPAMLMVWLLMTV
                     LGVSACSGLSNLPPAKPNLGSSSVIYAINIVATSGIVTINTQITLTVMQ"
BASE COUNT          847 a          973 c          988 g          777 t
ORIGIN      
        1 cgaaaatcac cagcgcccct acagcaacgc tctgatgatc gtcgacttgt cggaccccgc
       61 gaacgtgcag gaggtctcgc gctggtgggt gcccgggcaa aaattaggcg aggaagaaga
      121 atacaaaaaa tatcgcttcg ctggagacaa aatttcgtgg accggaaatc acggcgcgct
      181 caccgtgccc aagcgagtgg aggacggtgg cacgataggt tacggtggtt ttggagcatt
      241 cggcttctac gccatggact tgtccgacat aaaacacccg aagccttatg gccacgtgca
      301 atatgagttc gacacgtttg gcacgattcc cttccacacg tgttacccct taagtgggga
      361 cgcggcgcac ccgcaactca tgaacaaagt agtggccgtg cacgaggcac tggaagcgga
      421 ttgccgggaa aattatcaca cgccgtacat tctggatgtg aaggatccga aaaatccaaa
      481 aattatcgga ctctttccgc gacccgccgc gccacccgat gcgccgtaca cagatttctg
      541 tttcgcgcgc ggacgcttcg gttcgcacaa cacgcagtgc tggctcgccc ctggcacgtc
      601 gaatcccgct attatggtaa ttgcgtggtt caacgccggc gtccgcgtgt tcgatatttc
      661 caatcccacg gcgcccaagg aaatcgcgtg gttcgtgcct ccgcttgagg gagagatcaa
      721 caattacgaa tcctggtggc gcggcacaac ggaaaatgtg ttcgtcgagt tcgaccgcaa
      781 cctgatctgg atcgggaccc atgaaggaag ttactgcctg tcatgccccg ccctcggcaa
      841 ggccataacc gagcccgcca aggttgcgaa atggtccatc ccgcattgca atgtggggtg
      901 ggacgaccaa accgcgcgca gtttttattt tggccgttcg ttgagccaga tgggatagtc
      961 tgttcgtgga ccgaacactc gaggacggtt tgtgcggcgc ccgcctgaag gcgggcgccg
     1021 caactcgaaa aatcggtttg aggagaagtt cgtgaacaat agaaaagtct cacgtagaga
     1081 atttacatat ggggcgctcg ctgcgggagc cgttcttttc accggcagtc gttctcctgc
     1141 cgcggacttt gaactgcgcc aatttcacaa ccagcctgcg gacagtccgc tggatcaatg
     1201 gctttctaaa atgtgggccg gggtaaaaac agaaacgaat ggccgcgtac aagtgcaggt
     1261 ttttgctgat aacaataaaa ttgcgggcgg cgatcccgcc gcattaaaca tgctggtcgg
     1321 cggcgaggtc gattttttca cgttgaacgg cggatccatt ggaaacattg tgcccgcgtg
     1381 taacgtgcaa ggattgcttt gcgcatttcg ctctacacaa caaatttttg aatccctcga
     1441 tggcgatctc ggcgactatt tgcgggaaga aatgaaatcc aaaggcatct acggctttcc
     1501 caaaggctgc tttgataacg gcatgcacca gattacatgc gccacgcgcc ccatccgcac
     1561 cgtcgatgat ttgcagggcc tgaaaatccg gactccggac gctccgattt acacggaagc
     1621 gtggaaggcg atgggcgccg cgcccgtcgt ggctaacttc aacaagttgt acgaaacatt
     1681 gaaaaccggc gctgcggaag cgcaaacgaa cccgctcgcc gtcgcggagt tcctgaagct
     1741 ttacgaagtg cagaaatatg taagcgtgac caatcacggc tggtctggct tcaatctcat
     1801 ggcgaacctg aaaatgtggg agcgcatccc tgccgatcta caaaagatta tcgagcgcaa
     1861 cgttgtgaaa tttgtgcgcc agcaacgcga agaattaacc gcgctcaact cggaattccg
     1921 caccaaactt gccacgcaag gcatgacgtt taacgatgcg gacatttctt ccttccgccc
     1981 gcgcctggta gcatattacg cgcactggaa agaagtggct ggagaccgcg cgtggaaact
     2041 gctcgaagct cgaaccggca aactggtgta agtttgcttt ggtgacgcgg ctttagcccg
     2101 cattgttttg tctttgcaag cactaaaccc cgcaggctaa agcccgctcc gctaaaacct
     2161 gcgtatttgg gattttcgct accgtaaaag ttatttcgcg gcgggcgcag ttatattcag
     2221 ctgttcgcct tgatcgcgga atggcagctt gagtttgcgc gccgcctcag gccaaaactc
     2281 tcgcagttcg tcaaaaccga tatccagcgc gatgtcgatt ccgatgtttt gagccgcagg
     2341 cccgacgccc cgcccatttc cgggatggta taagttgctg attgaggcgc cgctgatctc
     2401 tcccagccat tcagagaaat taaacatcgg cctgccgctg tcgttataag ccaccaccgc
     2461 gtgctccacg gcgtgcagaa aacgcctgtt cgcaggcccc tgtccgaccc ggtagtatcg
     2521 aggatcttcc ctgaagatgg tcgggtaagc aaattcctta aaaaatcgtg actgcacctg
     2581 atccgtgtaa cttaccgcca gccgcagtcc atatcccttt gcaccctggc caaaggacgg
     2641 atcattattt tcagcctgcg aaattccggc gttgaatcct gcactgaaaa tcgtttcggg
     2701 atcgatggcg tcatgcagaa atagaaagaa tttgtccttt atctggagtg agcaaagcat
     2761 tgagccctgc ttataatgcg gatcatgaac agattggcgg tcgagttttt gcgtaaacgt
     2821 tccgactact ttgtgtaacg gtccgttgta atccttcatc tgaaccatgg gcggcggctg
     2881 cacgcagcgg cctgcggaat tcaacaccgc ttgccctggc agggtggccg gtgcgctgga
     2941 ctccggagga aggggaggcg cttcttggga aaatgccacg ccgctgatca acaaaaatgc
     3001 agatagaatg atcgcctgga ggacgctgcg agaggaaaca ccgcaaccga tgcggcccaa
     3061 ttctagtcta ctgattccgc cagatgcttt gatcgatcca attcgcccgg gactagccac
     3121 gttgccatta ttgcataact gtcagagtga tctgagtgtt gatggtaact attccagaag
     3181 ttgctacgat gtttatggca tatatgacgg aggaagaccc tagattcggc tttgcgggtg
     3241 gtaaattcga gaggccggaa cacgcactca caccaagcac agtcatcagc agccacacca
     3301 tcaacattgc aggcgcaaga ggacgcattt ttattttgcg gcttcgtccg cagaggcata
     3361 tccacaaagc gcccgcgacg agcaacgcac tgatcgacac catccaggta tcgcgcggcg
     3421 cgagtagaat tctcccgccc gaacctgacg aatgcggcgc ggtctctatc gagagaatta
     3481 tgccggtggt tccgcttccg gctgggatgc tggcgggagt aaaggaggca gttgcgccgg
     3541 gaggcagccc ggaagcggag agcgcaacgg aatgactaaa tgttc
//
LOCUS       DASZMB010000227         4726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_521623, whole genome shotgun sequence.
ACCESSION   DASZMB010000227 DASZMB010000000
VERSION     DASZMB010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521623"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..1204)
                     /locus_tag="VG272_10555"
     CDS             complement(314..1204)
                     /locus_tag="VG272_10555"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWF14166.1"
                     /translation="MGETKDACRIRGLLDTQGCGNFHISHVTDLDLAAERISTDPTDV
                     LLLDLGPRQQHCRTCVQAARSAAPDIPMVVLADYDDELLALEALQQGVQDFLSKERLD
                     RPTLVKALRYSIERHRLQRNLHDLSLRDDLTGLYNRRGFMALAEQHLRMLQRRGPALL
                     IYIDLDELKLINDVHGHLEGNRALIVTANVLRTCFRQSDILSRLGGDEFCVLMTDAGQ
                     DSADQVRKRLKSRIDFINGLTSWNFRISLSVGIADVPAMHQPPLEQLLRIADTQMYEE
                     KRNKRARNNESQIAKQSTVA"
     gene            complement(1503..4040)
                     /locus_tag="VG272_10560"
     CDS             complement(1503..4040)
                     /locus_tag="VG272_10560"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HWF14167.1"
                     /translation="MMRAQKSVWISLVAVLCALSLTLTRVSAQQEPPAPGRGESVADT
                     LEAIQKARVVTRVLFTVAHPDDEASTLLTYLPHALGTDTTLLTLNRGEGGQNAIGPEQ
                     GAQLGILRSSELSAAMNVEGPHLYFTRVVDFGFSKTLSETLEKWQGLELEDMVRTIRM
                     LRPQIVVNGWGGQKTGHGNHQASGYQTPKAVEAAADPTKYPDQIAEGLKPWRVETVMN
                     PVRATAPDEKVDYTGSWVVPADEISPIWGLSYRDISLEGYLHHRSQGVTPFINSPFVR
                     RPYGLKRSTGGAPSPSDFSQPLTSLSHVVPVPAEDSLAATDHDLDQARVAAEKLDWSG
                     AVRNIAEAGKRIVALEEQVKKSKDSSASNALWELEQVRGRINHALSDAAAIKIVSNAD
                     RSELVPGEGFSVRAEIAHRNDLPANFSKPELVLPEGWNITKREEKDGSITFSVAVPAG
                     AKTPHIPGEFLDPFPPPLVNACTHVEVDGYSFDFTAPVGSLHSTSVSVLTYPLRIVPP
                     VGITPEPQQYVVVESHQPQRFDIFARVHSFSLAPSKVNVGVEVPSGWKAPAPEDVEFS
                     GAGDRLVHLIVTPPQKIAAGNYILKAYVKRGDTTFENSLEPLPSLPTYLWSAPAAVPV
                     HVFSIAVPERLRVGYVSADNDGTISDSLRRLGVDVEMMDANALAFSDLSRFDSIVVGM
                     RAYELRSDVIASNWRLLDYAANGGTLVVLDQRPAVWDPLKPAPFSATMGPGPRVTDET
                     AAVNFTDPASPLLNFPNKIETHDFDGWVQERGLYFLEKWDEKYHTVLSMHDPGEKDLL
                     GSLVWARTGKGFYIYTGLSFSRQLPEGNAGAFRLFINLLSQTRNTAK"
     gene            complement(4037..>4726)
                     /locus_tag="VG272_10565"
     CDS             complement(4037..>4726)
                     /locus_tag="VG272_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWF14168.1"
                     /translation="AEIRDPEKNIPRAILISIAGIAVLYLAMQTSILGVVPWREAQNS
                     QYIVSTFVERLYGARAATFATIMILWIAFASLFTTLLSYSRVPFAAAEDGNFFPVFAR
                     VHPKKHFPHISLLFLGATSLVFSVLFRLSSVIKAIIAVRLIVQFISQGVGVMILRRHW
                     PPERLPFKMWPYPWPAVLAIVGWVALFYSTGRTFALGGLGVIALGIVTYMIQARYRRI
                     WPFAAVMEAQR"
BASE COUNT         1146 a         1307 c         1248 g         1025 t
ORIGIN      
        1 gatatacggg acgcctgcgc agctttattc cactagaacc cgaacacaga attgcagaag
       61 aggcgcaacg cggcgccgaa ctcgtagccc gagcgcccac aatccgtcca tggacatata
      121 cgtttgccat aaggcaattc gcaaaattca ggaaccgcat gtgctgtccg tcctttaact
      181 cagaaatgga atgcggagcg caatgcaatt tcaaatgcat tacgccgcga acactccaca
      241 agctgtctat cggcaggatt tggattgtgc ttgaggcgaa gtcgatgttc cgctatgggc
      301 catccgagga aagttatgcc accgttgact gtttggcgat ttgagactcg ttattacgag
      361 cccgtttatt tcgcttttcc tcatacatct gggtatcggc aatccgaagc aattgctcta
      421 gtggcggttg atgcatcgca ggcacgtcgg cgattcccac ggacagagat atccggaaat
      481 tccatgaggt cagtccgttg atgaaatcta ttcttgattt taaacgttta cgaacttggt
      541 cggcggaatc ttgccctgca tccgtcatca aaacgcagaa ttcatcgcca cctaatcgcg
      601 ataaaatgtc tgactgccga aagcaggtgc gcagaacgtt ggcggtgacg ataagcgcac
      661 gattcccttc caagtgacca tggacatcgt ttatcaattt tagttcgtcc aaatcgatgt
      721 aaatcaacag cgctggtccc ctgcgctgaa gcattcgcaa atgctgttcc gccagagcca
      781 taaatccacg gcgattgtat aagcccgtca aatcatctct taaagaaagg tcgtgtagat
      841 ttctttgcag tctatggcgc tcgatggaat atctcagcgc tttgaccaat gtcggtcgat
      901 ccaaccgttc cttggaaaga aaatcctgca ccccttgctg caaggcttcc aatgctaaaa
      961 gttcgtcgtc gtaatcagcc agaacaacca tgggtatgtc cggggccgcg gaacgcgccg
     1021 cttgcacaca cgtgcgacaa tgctgttgtc tgggccccaa atcgagcaac aagacatccg
     1081 tcggatctgt agaaatgcgc tcggcggcga ggtcgagatc cgtgacgtgg gaaatatgaa
     1141 aattaccgca cccttgagtg tctagaagcc ctcgaattcg acatgcatct ttggtttcgc
     1201 ccactagtaa gatccgaata ggactactat tcatggacac cgcgcagcct catgattccc
     1261 attcttagct cgaccattct gagattagcg attctgattt cagaatgaaa caaggcttgc
     1321 ctcgagacgg ttcattgcaa atcgcatggg gcctcggggc caagcgaaca tgggaattca
     1381 ctttctggaa gggggacttc tagtctcatg cacatgggaa atgttgtcaa gagatgtgcc
     1441 cggcgatgac gttttaaaga tactaccctc caaaattgtt aggggagggc ttccgagcaa
     1501 ctctactttg cagtattgcg tgtttgactc agaagattta taaacaggcg aaacgcccca
     1561 gcgttgcctt ccggcagttg gcgcgaaaag gaaagtcctg tgtagatata aaagcctttg
     1621 cctgtgcgcg cccacaccag gctgcccagc aaatcttttt cacccggatc gtgcatcgat
     1681 aagaccgtat gatatttctc gtcccatttt tccaggaaat agagaccgcg ctcctgaacc
     1741 cagccatcga aatcgtgggt ttcgatttta tttggaaaat tcaaaagcgg acttgcagga
     1801 tcggtgaaat ttacggccgc ggtttcatcg gtgacgcgcg gccctgggcc catggtcgcg
     1861 gaaaacggag caggttttag cggatcccag acggcgggac gctgatccaa tacaaccagc
     1921 gtgccgccat ttgccgcgta atcgagcaag cgccaattgg atgcgatcac atcggagcgc
     1981 aattcatagg cgcgcatacc gacgacaatg gaatcaaatc tgctgagatc gctaaacgcg
     2041 agagcatttg catccatcat ttcaacgtcc acacccaatc gcctcaggga atctgaaatc
     2101 gtgccgtcat tgtccgcaga aacgtagccg acgcgcaggc gttccggaac cgcaatcgag
     2161 aaaacatgca ccggcaccgc cgccggtgcg ctccaaagat aagttggcag cgagggcagc
     2221 ggctcaagcg aattctcaaa agtcgtatcg ccgcgcttca catacgcttt caatatgtaa
     2281 tttcccgccg caattttttg cggaggagtg acgatcagat gcaccaggcg gtcgcccgca
     2341 cccgaaaatt cgacgtcctc aggcgctggc gctttccagc ccgatggcac ttcgactccc
     2401 acatttacct ttgaaggcgc cagcgaaaac gaatgcacgc gcgcgaaaat atcgaatcgt
     2461 tgaggctggt gtgactcgac caccacatat tgctgtggct ctggcgtgat cccgactggg
     2521 ggaacaatcc gcaagggata agtcagcaca cttacggaag tggagtggag gctcccgacc
     2581 ggcgccgtga aatcgaaact gtacccatca acctcgacgt gtgtgcaagc attcacgagc
     2641 ggcggcggaa acgggtcgag aaattcgccc ggaatatgcg gcgtttttgc tcccgcggga
     2701 acagcaacgc tgaaggtgat cgacccatct ttttcttcac gcttcgtaat gttccagcct
     2761 tcagggagca caagctctgg ctttgaaaag tttgccggca aatcgtttcg gtgcgcaatt
     2821 tcagcgcgta cgctgaagcc ttcaccgggt acaagttcgc tgcggtccgc attggaaaca
     2881 atcttgattg ccgcggcatc ggagagcgcg tgattaattc gcccgcgcac ctgttcgagt
     2941 tcccacagcg cgtttgacgc ggaagaatct ttcgactttt tgacctgttc ttcgagcgca
     3001 acaatgcgct tgcccgcttc ggcaatattg cggactgcac cgctccaatc cagtttttcc
     3061 gcagcgaccc gcgcttgatc cagatcgtgg tcggtggcgg ccagtgaatc ttccgccggc
     3121 acgggtacta cgtgagagag cgaagtcagg ggttgcgaaa aatccgatgg cgagggcgca
     3181 ccgcccgtcg agcgcttcaa accgtacgga cgccgaacaa agggcgaatt gataaacgga
     3241 gtcacgcctt gcgaacgatg atgcaaatat ccttcgagcg aaatgtcgcg atagctgagg
     3301 ccccagatcg gagaaatttc atccgcggga actacccacg atccggtgta atccactttt
     3361 tcatccggcg cagtcgcgcg gacaggattc atcaccgtct ccacgcgcca tggtttcaag
     3421 ccttcggcaa tttgatccgg atacttcgtg ggatcggcgg cggcttcgac tgctttcggt
     3481 gtttggtaac cggaagcctg gtgattgccg tgaccggttt tttgtccgcc ccatccgttg
     3541 accactattt gcgggcgcag catgcgaatg gtgcgcacca tgtcttccaa ttccaggccc
     3601 tgccattttt ccagcgtttc gctgagcgtt tttgaaaaac caaaatcgac cacgcgcgtg
     3661 aaataaagat gcggcccttc cacgttcatg gcggcggaaa gttctgagct gcgcaagatg
     3721 ccgagttgag ctccctgctc cgggccaatg gcgttctggc caccctcacc acgattcaat
     3781 gtaaggagcg ttgtgtccgt gcccagcgcg tgcggcaaat acgtgagcag cgtggaagct
     3841 tcatcatcgg gatgcgccac tgtaaagagg acgcgcgtta cgacgcgtgc cttctgaatc
     3901 gcttcgagcg tatccgcgac gctttcaccg cggcctggcg ccggaggttc ctgctgcgcg
     3961 gaaactcgcg tcagcgtaag agacagcgcg caaagaactg ccacaagcga aatccaaact
     4021 gatttttgcg cgcgcatcat cgttgcgcct ccatgaccgc tgcgaatggc cagatgcggc
     4081 ggtaccgagc ctgaatcatg tacgtcacga ttcccaaagc aatcacccca agtcctccaa
     4141 gcgcaaaggt tcggccagtg gagtaaaaca acgccaccca acccacaatt gccagcacgg
     4201 ccggccacgg atagggccac atcttaaatg gaagccgctc cggaggccag tggcggcgca
     4261 atatcattac accaacgccc tggctaatga actgcacaat cagccgcacg gcaataatcg
     4321 ccttgatcac actcgataga cggaacagca cactgaacac gagcgacgtg gcgcccagaa
     4381 aaagaagaga tatatgcgga aaatgttttt tgggatgcac gcgggcgaat accggaaaga
     4441 aattgccgtc ctcagccgct gcaaacggca cgcgcgaata gctgagcagg gtggtgaaaa
     4501 gcgaagcgaa agcgatccac aaaatcatga tcgtggcgaa cgtggcggcg cgggcgccgt
     4561 acaaccgctc gacaaatgtg ctcacaatat attgcgaatt ttgcgcctcg cgccaaggca
     4621 ccacgcccag gatactcgtc tgcatcgcaa gatacagcac ggcgattccc gcgattgaaa
     4681 tcagaatcgc gcggggaata tttttttcgg gatcgcgaat ctccgc
//
LOCUS       DASZMB010000228        33727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1355467, whole genome shotgun sequence.
ACCESSION   DASZMB010000228 DASZMB010000000
VERSION     DASZMB010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 33727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33727
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1355467"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..684
                     /gene="tnpA"
                     /locus_tag="VG272_10570"
     CDS             178..684
                     /gene="tnpA"
                     /locus_tag="VG272_10570"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HWF14169.1"
                     /translation="MAHSYSQNHIHLVFSTKERRNSISKELQPLLWAYIAGICKKYEM
                     IAVAIGGAENHAHLLFHLSPKLALAKAVQLIKGNSSKWMGEQGIEFAWQEGYGAFSVS
                     SSNLEQVAGYIRNQEAHHRKISFEEEFRALLRSMAWNTIRNMSLDRGSARKKVKRVCR
                     PSGAFSSC"
     gene            950..2224
                     /locus_tag="VG272_10575"
     CDS             950..2224
                     /locus_tag="VG272_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14170.1"
                     /translation="MTRYKWICAGAFGLLFAFGFLFVKGPAAQTAEVPKFKADLTWPK
                     ALPNKWKMGGVTGLAVDKDDNVWVYDRPDDLTSVEVEAELNPPIADCCVHPPSMIHIA
                     GHDGPGYKDGDVIGSFEAPQGHGMAVDKQGFLYLGQNDVRKYDPKTGKMLMAVPHVPE
                     TPDGKPFMEPMLPKRAPGEGTAGPIAGFITIPGTERRARGEAAGTAAGLARTAFITKY
                     PPTTPMIVGGIEEIRTDDAANEIYIADNAFGGRVMVFSLDKFEFKRGWGAYGHKLSEI
                     STAPEDHAYKPNGPMAKEFVGHLTFNFSVDGLVYAADRGGNRIHVTDKQGNFKQEIKM
                     AEWTGVGGSTGGVMFSNDKDQRLLYISDLTNNHIWFLDRKTGKVLGTIGSMGQNAGQF
                     FGVHMEATDSKGNLYTGEVFNGERVQRFLLQK"
     gene            complement(2334..2963)
                     /locus_tag="VG272_10580"
     CDS             complement(2334..2963)
                     /locus_tag="VG272_10580"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HWF14171.1"
                     /translation="MTTENYNPKVDAYIAKVQPFAEPILNHLRELVHKGCPGVEESIK
                     WGMPFFEHRGEILCCMAAFTKHCRFGFWGKEIRVVLREAKVTGMNSTGWFERITRADE
                     LPTDKRMIDWVRQAAVLIESGNHTSPIAARNKKAKKPTVEMSKEFATALRKSKKAKVV
                     FAAFSPSCKREYVDWIADAKRPETRDKRITTAIEWISQGKQRHWKYQNC"
     gene            complement(3139..5166)
                     /gene="argS"
                     /locus_tag="VG272_10585"
     CDS             complement(3139..5166)
                     /gene="argS"
                     /locus_tag="VG272_10585"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF017555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HWF14172.1"
                     /translation="MVNVLVARVRAALAAHIRRHYKLDVTIVTEKPPRIEMGEAATPV
                     CFELAKRLKRAPRQIAQEIAVQLPPIEGVARVEVAGAGYVNFYFSRTAFLASSLKDAD
                     TRRPGFAPDAPKCIVEHTNINPNKAAHIGHLRNAVLGDTFVRLLRRAGQCVEVQNYID
                     NTGVQVADVVIGFLHIAKKSAAEVRALAAEPKFDYYCWDLYASVSRFFDEDKTRLNLR
                     GETLKSIEKGAGEASEMAGIVAPAIVRCHLRTMERLEIEYDLLPRESEILHLKFWDAA
                     FDLLKKRNAIHLATVGKNSGCWVMRMNDGSDADEPSSDPPGITASNASAEPAVEDDDA
                     KVIVRSNGTVTYVGKDIAYQLWKFGLLGRDFHYEKFYRYPAGHTLWSSTATAGDSSAP
                     AFGRAAIVYNVIDARQAYLQNVVTAGLRALGFEDQAARSIHFSYEIVALTPRCAAELG
                     YSLSEEDAKKPYVEVSGRKGMGVKADDLLDRLEAAARAEVDERHPGTPDMERAAIAHS
                     IAIGALRYFLLKFTRTATIAFDFKDALSFEGETGPYCQYAVVRARNIFRKLREQQPDF
                     DLSSLENVDAIAAAELFAGPEGNAFWELAFLAASLDTQIEASVNAQEPAFVAKYAFQL
                     AQAFNLFYHHHHILTESDAAKKKFLLQLSRLVEVQLVAALELLGIESPEKM"
     gene            complement(5481..6821)
                     /locus_tag="VG272_10590"
     CDS             complement(5481..6821)
                     /locus_tag="VG272_10590"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HWF14173.1"
                     /translation="MPSKFLRRIPAALWIAPMAVGLMFSTTARAQNSASAPQVLRGEG
                     SFNDWNNERPGNRYTIKASDLPKPYATDSAPNQSKVVPRPADAWPKVPDGFKIDQALT
                     GLESPRTIITAPNGDLFVAESTPGRIRVLRGFGSDGKVVTNDVFATDLTMPYGIAFYP
                     PGPNPQFVYIGNTNSIVRFPYKNGDLKASGPAQVIVASIPGGARYGGGHWTRSLVFSN
                     DGKKLFVGIGSKANVGDDQSETNRADVLEFNPDGSGQHIYAAGVRNASGLTMHPQTGQ
                     LWVAVNERDALGDNLVPDYVTHIQEGGFYGWPWYYTGPNQDPRFAGKHPELKEKVLVP
                     DVLLQSHSAPLTLTFYEGKQFPAEYRGDLFVTSHGSWNRAKRTGYKVIRVYTPNGKAT
                     GEYQDFVTGFVVDDANVWGRPVGVTVASDGALVFTDDASNSVWRVTYTGRQAGN"
     gene            7205..7864
                     /locus_tag="VG272_10595"
     CDS             7205..7864
                     /locus_tag="VG272_10595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF14174.1"
                     /translation="MAEPVETRDIELLGRLKAGDEDAFLEFYRRHQGGLYRYAMHMSG
                     SPQAAADVVQETFLSLIRHAGKYDQEKGNPVAFLFGIARNHLRKLYEKESRYIPLSDE
                     LSSGLSADGANHSRHSNGNGHSPVIMGQSETIQEGLEREQIGELLRDAILTLPDHYRE
                     PVTLCDLEGKSYGEAAALLDCPVGTVRSRLNRARSMLLDKLRPAKIGVRAQWARPGGK
                     A"
     gene            7861..8562
                     /locus_tag="VG272_10600"
     CDS             7861..8562
                     /locus_tag="VG272_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14175.1"
                     /translation="MKEMTCSGFDEVVHGFVHMELLDVTVRESALEHAAHCALCSERM
                     ADATALAEASEAMSQNARDKQAPPYVEAALLVAFRGHHRRASWQHTLEWASVGAAAAV
                     LILFLWLGNGKQKMQSAPGPRKDASSQSTVPVLAAVSEPLKMDSAPATTTAKAASADT
                     SKNETYLASDFVPVPYTGPIVADDPGMVVRVQLTRASLAQLGYPVAETPDEDLILADV
                     LVGEDGWPRGVKLIQ"
     gene            8618..9460
                     /locus_tag="VG272_10605"
     CDS             8618..9460
                     /locus_tag="VG272_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14176.1"
                     /translation="MRKVGLRLSAGAMLLGGMAMVVSAQGPPPPGGFGGGPKPGIFEI
                     GGLMGGFGGKVVTGKPILATITITHTETLPGNLISNTSTGTYARGADGSTYRDVKFSE
                     IGPWAASGKAKEFAYIRNVTTGMEYIVHVTEGTYQAFPLRERNASPGSKFGPPGGPNP
                     NDENVTDNPNGSYTDPATSTVYPADDKQVTRTIPAGAIGNTNAIVITSERWFSNAFEI
                     VFEDTHTDPRFGTTTYKLSNIGQSASTSLFTPNPTFTQVQGGGFGKRGPHGPGKQPPP
                     PPQD"
     gene            9893..11908
                     /gene="metG"
                     /locus_tag="VG272_10610"
     CDS             9893..11908
                     /gene="metG"
                     /locus_tag="VG272_10610"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF008900.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HWF14177.1"
                     /translation="MSTPRAKPKYYLTTPLYYTNGMPHIGHTYSTIVTDVIRRYKRMR
                     GFDVFMTTGADEHGVNVERSAQKAGKSPLDFSTEIAVEWKQLWDDLGIAGDEFIRTTD
                     EKHHRTVQWLFKRCRDNGFIDKGSYTGQYCVHDNAYVVEAGPGDPCPDCGRPTETVTE
                     ENYFFKLSEFQQKLLDFYAANPDFIQPESRRNEVLSFVNGGLKNLSITRTTIKWGIPV
                     PGEFPHVFYVWFDALIGYLSAVGGPDYDQCGLWPADLHIVGKEIIRFHAVYWPAFLMA
                     ADLPLPKQIWAHGWLLFDSQKMSKSRGNVVRPRPIVNALGIEPLRYYMLREIVFGQDG
                     SFSYDAMVTRYNSDLANGLGNLASRTLTMIERYFGGEIPKPAVTQGSDMYVPAAADFA
                     AEALTNITKLYDRFSFSQALEAIWEQVAAIDKFITVEKPWALADKPENRDRLAMVLYS
                     AAESLRIIAALAHPVLPEATRKIWNQLGLTEPLGQAQLDQIAWGQLKPGTKIGKPEGI
                     FPRVDKSETIARIEAMEQEPTKVPETATAPAATPTTAAATAPATTPAAAAAAPIVAKI
                     GIEDFAKVEMRVGLVKSAERVAGADKLLQLQVDIGDEVRQIVAGLALAYKPEDLVGRK
                     VVVVTNLQPRKLRGVESNGMIVAASLPDGAPVLAGFIEDVPVGAKLK"
     gene            11908..12354
                     /locus_tag="VG272_10615"
     CDS             11908..12354
                     /locus_tag="VG272_10615"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HWF14178.1"
                     /translation="MHGSLFFLKGLLLGFSIAAPVGPIGLLCIQRTIAYGRSSGLATG
                     LGAATADGVYGTVAAFGLTAISGFLVGQQFWFRLIGGIFLFYLGAKTLFSRAAEKAAS
                     SNHNSLFSDYASTVFLTITNPMTILSFAAVFGASVLPTQADTGLPC"
     gene            12670..13500
                     /locus_tag="VG272_10620"
     CDS             12670..13500
                     /locus_tag="VG272_10620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HWF14179.1"
                     /translation="MDLIDSHAHIDVSDFDADRGAMLDRARAAGVGTVLAIGNGPELE
                     KLGAAIPYAEANDWIYAAAGIHPHEAKQATDAHYSELERIARNPRLIAWGEIGLDYHY
                     DHSPWDVQANVFRHQLSQARAAKKPIIIHCREAWPDCLNILDEDWRSSGLGGIFHCFM
                     GTLDEARRGMDMGFMISFAGNVTYPKAQNLRDVANEIALDRLLVETDSPFLAPQSHRG
                     RRNEPAYVAEVAQTIGNVRNLPADQIAEITSANFRRFFGLGESGNRNKAVPGGASSHF
                     "
     gene            13524..14471
                     /locus_tag="VG272_10625"
     CDS             13524..14471
                     /locus_tag="VG272_10625"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWF14180.1"
                     /translation="MRDDLVKVEQELALQNGSAIEPVAEISRYLLEGGGKRLRPALLL
                     LSAGYAGYRGESAIRLGAVVELIHSATLIHDDVIDGANTRRGRPSANSRWGNHRSVLA
                     GDWLYMQSFQMALTERNFKILDVLIELTQNMVEGELVQLAKLGRMDLTEQDAVELAAR
                     KTACLFAGCARLGAVLGNLSDTGEQAMADYGHYMGLAFQLVDDVLDFTASPEKLGKPV
                     LSDLKEGKVTLPLIYALETDGEEGKRLVRTVLEEKGFDSVRPEEITELVHKSGALDRT
                     RAAAFDFASRAKACLNGNATSDYGRALRTVPDFILDRDN"
     gene            14499..15425
                     /locus_tag="VG272_10630"
     CDS             14499..15425
                     /locus_tag="VG272_10630"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HWF14181.1"
                     /translation="MLPFKLIYHPQYDLDLGTHVFPSQKFRLIHEALRAEGIAEASDF
                     LRPEPAPNEDILRVHTQAYVNKLLTDSLTLSERMKLEIPLTELTRNGFWLAAGGSTLA
                     GQKALEDGFAANLGGGFHHAYPGHGEGFCMIHDVAVAIRSLQAAGKIRTAMTVDTDVH
                     HGNGTAAIFMEDPSVFTLSIHQQNNYPEPKPPSDEDIGLADATRDHEYLDALEKGLLR
                     SFKKVSPDLIFYVGGADPYREDQLGGLALTLEGLQQRDKLVFDHVRRRGIPVASTLAG
                     GYARRVADTVRIHVNTIIAARDVLLRQIPAGS"
     gene            complement(15574..16959)
                     /locus_tag="VG272_10635"
     CDS             complement(15574..16959)
                     /locus_tag="VG272_10635"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HWF14182.1"
                     /translation="MRYPTRLLLCLLAFSFAGIARAQQSPSSASPAKAPAKKSSAPSQ
                     AVPKPKAETTPAEPQDSAPDQDAELQILVRQAGNDPATLVKNLESYLSRYPDSPRRAA
                     IYRALIESEMNLHNSQAALDYAEKVIAIQPEDSQSAYLAVTLLEKMSGDANQLKAIDY
                     DTRLIDRVSKADPDSRPQQMTLEDWQSGRNKFLVDLYVLRGRVQRHLHKNDDAVKDLT
                     VGFRMLPNAEAALTLGEIAEEEKQSDEAIRQYASAFMLAGQEQSDSNGPPMDREMLRL
                     RMGNLWRYTHPSTAGLGDIMLSAYDRSREALKAEKADVPVYNKDVSDALQFSLRHVDG
                     SGTVKLSESHGKVVVLNFWTTWCAYCNSMESMLAEVRTKFASRDDVVFLTVNADEDEN
                     LVAPYLKEQKFGGASVFADRINQAFHVESIPTVLVLDKSGKIAYRTQGFAPDGFTDLA
                     VAAITKASAAP"
     gene            complement(17156..18472)
                     /locus_tag="VG272_10640"
     CDS             complement(17156..18472)
                     /locus_tag="VG272_10640"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HWF14183.1"
                     /translation="MKRYLLACLAFHFLFVAAVAVRAQAPPANPAPSSTINSIKVTGL
                     QKFPQDQVIAASGLKNGDVVTAAQIQDATNLLAALGIFSAVNFRFTSKGTAIDLEFQV
                     TEAPTYPISFDNFPWFTSAEIGDAIRSQVGMFTGEAPDSGSMIDQMTAVIENLLGSKN
                     IKGSVTHQLISVPAGDGMEMQFRVEGVPLRVQSLKIDDPIAADSERLKDRIPDIKGQP
                     YSLFAVELFEYEQVRPLYITKGFLRAQIGPPQTQLVPDVNDPKVSEVDISIPVRQGSA
                     YTWKGVSWQGNMAQPSSTLDSLVKLKPGDLADGIKIENIWRDITFEYMRRGYLDLKLD
                     AEPQLDDTAHQVSYHVAITEGPQYRMGEMIITGLSVDAEKRLRQAWQIAPGQVFDNNY
                     YEAHMIIYAKPSREVFGELPVHYNEFGHLLRRDANRHVVDVLLDFK"
     gene            complement(18602..20467)
                     /gene="glmS"
                     /locus_tag="VG272_10645"
     CDS             complement(18602..20467)
                     /gene="glmS"
                     /locus_tag="VG272_10645"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HWF14184.1"
                     /translation="MCGIVGYIGSKKVVPLILDGLRRLEYRGYDSAGIAVVGSDGKVH
                     IRRAPGKLHNLEEVIRQLPVDGSYGIGHTRWATHGRPTEENAHPHRDCTGNIVVVHNG
                     IVENYVDLKRELESEGHKFVTETDTEIIAHLVERYMKEGGPLEDAVRNTVRRLTGVYS
                     IAILSAADPNKIIAARTGPPSVIGLGQNEFFVASDIPAILAHTRDMFFLADGDIAVLT
                     PDGVRVMDLDGNTIQRAVQHITWDPIMAEKGGFKHFMQKEIFEQPRAVRDTLLASVAL
                     DSGKVFLDKMDIQECEFRDFTKVKIVACGTSWHAGQTGKFMIEKLAKIPVEVDYGSEF
                     RYRDPIVDSKTLTICISQSGETADTLAAQREAKQKGSKTLAICNVYGSMITREAAGTI
                     LTHAGPEIGVASTKAFTGQLTALVLLAMYLGQVRGTLTEDCEKLLLQEVMRVPHKMET
                     VLQGDEAGIYEGLARTFFRHTNFLYLGRGIHFPIALEGALKLKEISYIHAEGYPAGEM
                     KHGPNALIDENLPVVMLATRDENDPESMTRYEKSVSNIKEVKARDGIVISVVSENDHL
                     AREASDYVIVVPPTMELFAPMLEVIPLQLLAYHIAVRRGCDVDQPRNLAKSVTVE"
     gene            complement(20701..20943)
                     /gene="purS"
                     /locus_tag="VG272_10650"
     CDS             complement(20701..20943)
                     /gene="purS"
                     /locus_tag="VG272_10650"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HWF14185.1"
                     /translation="MKAHVWVMPKRTVLDPQGQAIQHALSSLGFSEVSDVRQGKFFVL
                     DLNGVARESAEKQVERMAKEILANPVIEEFRYEIVE"
     gene            complement(20952..21986)
                     /gene="add"
                     /locus_tag="VG272_10655"
     CDS             complement(20952..21986)
                     /gene="add"
                     /locus_tag="VG272_10655"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HWF14186.1"
                     /translation="MNTSISEQVLTSIPKAELHIHLEGAIEPSTLVELAARHGVTLTP
                     EETAPRYAPGDFDQFIQNFIWITSFLRTPEDYALIAQRFAESMQRQNVLYAEVTLSIG
                     VMFRRNQDPAANFAALCDAAAKVAGIKLKYIFDAVRQWGAAPAMEVARIAAELRSPDI
                     VAYGIGGDELGLPTNDLIPVYDYVASQGMHRLIHAGEIGGPEIVRESVELLGVDRIDH
                     GISIMRDERTMDFIAARNIPLDVCPTSNLRTGALARQISQPTAGYEQHPLPLFVRRGL
                     PVTLSSDDPAMFETTLTNEYAQAQRMGMTPGEIVQLAEASFVHSFLPPNEKQAMLEKF
                     HTRISALDLV"
     gene            22554..23921
                     /locus_tag="VG272_10660"
     CDS             22554..23921
                     /locus_tag="VG272_10660"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWF14187.1"
                     /translation="MTESKSAGKRLAGHGRLITTLGVGFGIAVAIGNTIGAGIVRAPG
                     VIANLLPSTWLYFAAWAVGGLYMSLGALQFAELGALLPKSGGQYNFSRAALGDYAGFI
                     VGWSDWISTAGSFAAVSIVFAEYAGDLLPFAHGRVRPIAAGVILLFALLQWKSMKIGS
                     GVQNFTTILKAVLFLAVVAVCFFHQATPVTALASRAAVPVGWPLFVSFIVALQAVIYT
                     YDGWYGVVYFGGEVHNPGREIPRAIFGTVISVLAIYFLVNAAIVHLVSVREFAGSDFA
                     MGIAARRVLGAAGDNFIRVVVVVGLLSSVNAMEMATSRALYAMSRDGLFFPLFARVNS
                     GGIPAPSLWLSTAVGLCFAMFSFERVAAILASFFVADYALTFVSLFVLRKRMPDAERP
                     HRAWGYPWTTSIALIASVVFLIGALASDRENTPLTFLVLAASYPVYRALKWAARRSAE
                     RTTLT"
     gene            complement(24155..26419)
                     /locus_tag="VG272_10665"
     CDS             complement(24155..26419)
                     /locus_tag="VG272_10665"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWF14188.1"
                     /translation="MTLLDELNSGQREAATTTEGPVLILAGAGTGKTRAITYRIANLI
                     EQNVPGSSILAVTFTNKAAAQMKERVRDLLIRQGKAFDDPWIGTFHAFSAWLLRREAK
                     RLGLPRDFKIFDADDQVAAVKLSLDHLNIKDPGEKIRGLLERISSAKNHAQTPVQVAA
                     EAARRGDPLGITAAKVYGEYENILRKAGALDFDDLLLRAVQVLREFPEARAAWQSRFR
                     YIHVDEYQDTNRVQYDLLKLLVNEKSNLCVVGDEDQSIYRWRGADIGNILRFEEDYPG
                     ARVVRLEENYRSTQVILDAAAGVVSNNRRRLGKTLKATRSAGRNLAFFEGRDSKAEAE
                     HVADRMRMLHGDDPAVHIAVLYRTNAQSRSFEEALRSRGMRYRMLGGFSFYQRAEVKD
                     ALAYARLALFPDDDIALLRVINTPPRGIGKTSIDSLSALAREQDISLWTAIGKALDPS
                     FSGRALAPLRDFRILIEELQKEVTSSTPADFLQSILTRSGYLDMLLQRDNVEDTARAD
                     NLRELVNAMAEGAERGETLTDFLDHAALVSDADNFDEDALITLMTLHSAKGLEFEHVF
                     LTGMEEGIFPHSRSQNDNEELEEERRLCYVGMTRAKTTLTLTRAVYRRMYGSERLSGS
                     MPSRFLQEIPAELIDTAAGSLAEAGETRRYEPDPEYSYSQEEFSRRVKRVDSPSRSSS
                     AVASRPRSSSVSTRGGKANSNPLIGQRVRHATYGTGTIVAVEGDEPDRKFTVRFADHG
                     TKKLVEQYANLSWA"
     gene            complement(26499..27113)
                     /locus_tag="VG272_10670"
     CDS             complement(26499..27113)
                     /locus_tag="VG272_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14189.1"
                     /translation="MMLLPVRALAQSAPADPMSLHTRDAHQDLTIIADPYLKVDRYKK
                     EIFGKDSFYNAGIIAIDVYFKNDSSAPIRLNGDTIELIISQPDHDRQRLGPLSPEDVA
                     DRTLLKAESNIREHRPFPFPGTGSGSGHSKDWVQMTTALRAVALGTDVLPPHATTHGF
                     LFFDLNHQFDAIHDSHLYIPDLAFMTDKKALFFFEIDLAEAPQR"
     gene            27333..28712
                     /locus_tag="VG272_10675"
     CDS             27333..28712
                     /locus_tag="VG272_10675"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HWF14190.1"
                     /translation="MARREPYRMYAIYSLLTAAGMLLLSPYFLLRGLVQKKYLSNLPE
                     RFGRKFPSELLAGNTPGSKEKSIWIHAVSVGEVLAVLPLAQRLKKKYPQRRLVVSTTT
                     LAGQTLARERLLFADAIFFFPLDWRGPVRRALAAANPAAVMIVETEIWPNFLRECRRA
                     NVPVAFVNGRLSERSFRGFKRALSYSAGMLSGFLKKVLDDATLFLMQSKQDAARLVAL
                     GAPPDRVTVTGNLKYDLAEPDASPVSAWLEVEIARSDRRPVIVAGSILANEETPVLQA
                     FAEVEGEFPRALLILAPRKPEQFDNAATIVCESGRRLLRRRELSLNGVGSSILADPGN
                     VLLLDSIGELAGLYRLADAVFVGGSLVPSGGHNILEPAAFGKVPIYGPSMENFREMAG
                     KFLAAGAAIQVRNPKELGAAWRTLLRDSQTANRMGTLARELVDLNRGATDRVLGQLEH
                     LLADERSLK"
     gene            28709..29737
                     /gene="lpxK"
                     /locus_tag="VG272_10680"
     CDS             28709..29737
                     /gene="lpxK"
                     /locus_tag="VG272_10680"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HWF14191.1"
                     /translation="MKHASPWLRLLYPLSLIYSAIVRAKSWCYARGIFRGRKLPGTVI
                     SVGNLTVGGTGKTPMVLAIAERLAEEGKHASILTRGYRGTKATDETGIPQSDEVALLR
                     ERLAGKVQLGVGADRYKNGLVLSKHGVDWFVLDDGFQHRKLVRNADVVLVDAMDPFGG
                     KMVLPAGRLREPLSAMKRADLVVITRSVQTPAPALEAMLRRHTNRPIFYASTRLLNVS
                     RIPGMDVSLPEEVWRKARFLAFCGIGNPAAFFGDLRTWSLQAVQERSFADHHLYTPEE
                     AAELEQAAISSGADALLCTEKDVWNLRNVKFEKLPVYCCQISFELPENFWSAVSEAVH
                     RGKAGETQ"
     gene            29734..30798
                     /gene="waaF"
                     /locus_tag="VG272_10685"
     CDS             29734..30798
                     /gene="waaF"
                     /locus_tag="VG272_10685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HWF14192.1"
                     /translation="MRILVRAPNWVGDAVMAVPALEAVRDTHANDEIYVLARPAVADL
                     FAGQPFADKVALYDFRGKHAGWWGREKLIADLRNEKFDVAILLQNAFDAAWLTWRSGI
                     SERIGYARDGRRPLLTKAIRVPAQGEIPRHESFYYLELLRRAGWIETRSEIAPIRLQI
                     SEESRSSAESMLRTAGTRENAWRCAIAPGASYGAAKCWPPEYFAQLADRLISECDADV
                     IFFGTPGEKEIAARIGAHMKSRAISLVGETSMRDLAAMFASCSVFIGNDSGAMHVAAA
                     AGLPVIGIFGSTDPEGTAPVTQQFTLIREAVSCSPCFLRRCPVDHRCMTRIRVDSVFT
                     AAMQIKNSPNQVSIRGAKNA"
     gene            30791..31381
                     /gene="gmhB"
                     /locus_tag="VG272_10690"
     CDS             30791..31381
                     /gene="gmhB"
                     /locus_tag="VG272_10690"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: protein motif:HMM:NF006506.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HWF14193.1"
                     /translation="MPEKQILRRAVFLDRDGTISEEMGYVNHIDRFQIFPYAAEAIRQ
                     LNQADIPVIVVTNQSGIARKIFPESLLHEIHKKMVLELAKGGAWIDAIYFCPHKKDDA
                     CDCRKPNPGLMERAAREHGLDLSASWIVGDRYGDLEMGHAAGARGILVKTGYGRGEYE
                     LHHEKWPRQPDALAENLSEAVRLILQNGGSAPRRNE"
     gene            31374..32390
                     /locus_tag="VG272_10695"
     CDS             31374..32390
                     /locus_tag="VG272_10695"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWF14194.1"
                     /translation="MNKHEDRFLVVRLTALGDILHTVPAVAALRAAHKNAKIDWVVER
                     KWAPVLEGSPAINEVIPFDRRSTWGALECVARLRENRYSCAIDFQGLYKSSILASLSG
                     ASCRIGFERAWAREEGAAMLYTERVSPVGRHVAELNYSLAQRAGASHPQTPEYPLRVP
                     AGGAASVRARLHDLGITGDYVVVGPGGSWRAKCWPSERYGEFCRELERKFGMRVVVIH
                     GPGEKQIAEEVLRAAAPAQPTLISTTIEELMGLLAHARCLIAADSGPLHLAAALGTPV
                     VGLYGPTDPARNGPFVSNVVIVHKARLEEISYKRRVEYSPAMLRITTEDVMAATESLL
                     KVPA"
     gene            32399..32950
                     /locus_tag="VG272_10700"
     CDS             32399..32950
                     /locus_tag="VG272_10700"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HWF14195.1"
                     /translation="MSNKTNFWIRWRVRVGYPVGIAAFWFAHPQVKWLICGVSIAIVG
                     LIIRGYAAGHLRKHQQLATSGPYAYTRNPLYLGSVLLAAGFSVAGHSWISTLLLAAYL
                     AIFYPVVIRREQTELQAHYGDAFVEYASMVPAFWPRLSPAMASSEKFSWPLYLKNREY
                     EASIGLAVAMTILLALMFWRGGI"
     gene            complement(33056..>33727)
                     /locus_tag="VG272_10705"
     CDS             complement(33056..>33727)
                     /locus_tag="VG272_10705"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HWF14196.1"
                     /translation="WSRGIKRIDVVALTHAHEDHLGGLPAIFENFRVGELWVGRDIHS
                     AAYEQLIAQATAHGVIIKHLKQGDNFSEDGVSGSVLWPEDDAEGRSAKNDDSLVLRLN
                     NGAESFLLAGDVERPSERTILAEAQPVGVNFLKVAHHGSKTSTTDAFLSAAHPAFAAI
                     SVGRENSFGHPSPEVTDRLIAAGVRVYRTDRDGAITASTDGSTLTVSTFLRAAASQSF
                     STAKK"
BASE COUNT         7310 a         8677 c         9910 g         7830 t
ORIGIN      
        1 cggaggggcg acacaggtgc ttggccgagt gtgtcgcccc tccggggctt tcttctcggg
       61 cttacgtgtt cccacagctg aagctgtggg ctagggtgtt tcgtccctcc gggacttggt
      121 gaagttccgc ttgggacgcg gattgcggta gtatcaatac cccggagggg cggcacaatg
      181 gcccactcgt attcccaaaa ccacatccat ttggttttca gcaccaagga gcggcgcaat
      241 tccatttcaa aagaattgca gccgctcttg tgggcctaca tcgcgggaat ttgcaaaaaa
      301 tacgaaatga ttgcggtcgc gattggcgga gcggaaaacc acgcgcatct tttatttcac
      361 ctttcgccca agcttgctct ggcaaaggcc gtacagctca taaaggggaa ttcatcgaaa
      421 tggatgggcg aacaagggat cgaatttgca tggcaggaag gttacggagc attcagtgtc
      481 agttcgtcaa atctcgaaca ggttgcgggg tacattcgca atcaagaagc gcaccaccgc
      541 aagattagtt ttgaggaaga gtttcgcgcc cttctcagaa gcatggcgtg gaatacgatc
      601 cgaaatatgt cgttggatag aggatcggct cggaaaaaag tgaaaagagt gtgtcgcccc
      661 tccggggctt tctcctcttg ttgatgcatt cccagcgctg aagcgctggg ctacgttgtt
      721 tcgtccctcc gggactaggc aactgtggac tacatcattt cttctcgctg acgctgggga
      781 attgtgggga aatctgggct acattgtttc gtcgctccgg gactggcaaa tgcggattga
      841 gactcagacc ctcgcatctt tctccccgtt gaccttcctg cttgcccgga atagactgcg
      901 cgcaccaatc cgtgggtgat gaatacattc gattgaggag gaatgtccga tgacaaggta
      961 caaatggatt tgtgcgggtg catttgggct tctatttgct tttggatttt tattcgtaaa
     1021 ggggcctgca gcgcagacag ctgaggttcc taaattcaaa gccgatttga cttggccgaa
     1081 agctttgccg aacaagtgga agatgggtgg tgtcactggg cttgccgtcg acaaggacga
     1141 taacgtttgg gtctatgacc ggcctgacga cctgacgtcg gttgaggtgg aagcggaact
     1201 gaatcctccg attgctgatt gctgcgtgca tcctccgtcg atgattcaca ttgcggggca
     1261 tgatggccct ggttacaagg acggcgacgt gatcggttcg ttcgaagcgc cccaagggca
     1321 tggcatggcg gtggacaagc aaggttttct ctacctcgga cagaatgacg tgcgcaagta
     1381 cgatccaaaa acaggaaaaa tgctgatggc tgtgccgcat gtgccggaaa cgccggacgg
     1441 caagccgttc atggagccga tgcttccgaa gcgcgctccg ggcgagggaa ccgctggccc
     1501 catagccgga ttcattacca ttcccggtac cgagaggcgc gcgcgcggcg aagcggcagg
     1561 aaccgcggca gggttagcgc gaaccgcatt catcaccaag tatccgccga ccacgccaat
     1621 gatcgtcgga ggaattgaag agattcgcac ggacgacgcg gcaaacgaaa tttacattgc
     1681 ggacaatgct tttggcgggc gtgtgatggt tttctcgctc gacaagttcg aattcaagcg
     1741 tggctggggc gcgtacggac acaagctttc agaaatcagc acggctcccg aagatcacgc
     1801 ctacaagccg aacggaccga tggcaaagga atttgtcggc cacctgacgt ttaatttttc
     1861 ggtcgacggt ttggtttatg cggcggaccg cggcggcaat cgcattcacg tcaccgacaa
     1921 acaaggcaac ttcaaacagg aaattaaaat ggccgaatgg acgggcgtag gcggctcgac
     1981 aggcggcgtc atgttctcca acgacaaaga ccagaggctg ctgtatatct ccgacctcac
     2041 gaacaatcac atctggttcc tcgaccgcaa gaccggaaaa gtgctgggca cgataggcag
     2101 catgggccaa aatgccgggc agtttttcgg tgtccatatg gaggccaccg actcaaaggg
     2161 caacctctat accggcgaag ttttcaatgg cgagcgagtg cagcggtttt tgttgcagaa
     2221 gtaacgcctg tttgacgcag gaggttttcg tggcgccggc gagacgccgg cgccacgaaa
     2281 gctgacgcat tccacgcaat ctttatttca ataattgcga agacaaatca ctgctagcaa
     2341 ttctgatatt tccagtggcg ctgcttgcct tgggaaatcc actcgatggc cgtggtgatt
     2401 cgtttgtcgc gcgtttccgg gcgtttcgcg tcggctatcc aatctacata ttcgcgtttg
     2461 caacttggac tgaacgctgc aaaaacgact ttagcttttt tgcttttccg taaagccgtt
     2521 gcgaattcct tgctcatttc aaccgtgggc ttcttcgctt tcttgtttct tgccgcgata
     2581 gggctagtat ggtttccact ttcaattaat acggcagcct ggcgtaccca atcgatcatg
     2641 cgtttgtcgg taggaagctc gtccgcacgg gtgatgcgct cgaaccaacc cgtgctgttc
     2701 atcccagtga ctttggcttc gcgcaaaacg acgcgaattt ccttgcccca gaacccaaat
     2761 cggcagtgct tcgtaaacgc cgccatgcag caaagaatct cgccgcgatg ctcaaaaaag
     2821 ggcatgcccc acttgatcga ttcctcgacg ccagggcatc ccttgtgaac cagttcgcgc
     2881 aaatggttca ggatcggctc cgcgaacggc tgcactttgg caatgtatgc gtctacttta
     2941 ggattgtaat tttccgtcgt cattgtgatc ttcttccgga acgccaaata tattagcttc
     3001 tattttgcgg gtaacgctac tcgaagtttt cagcatttgc caaacgtgat tagcgccacg
     3061 aaaccatgtc cgtccaaaaa ttggatttag attaaattgc aaatcggaaa aaaattactc
     3121 aaaccgcacg caaaaacatt acatcttttc cggagattcg atgcccagca attccagcgc
     3181 ggcaactagc tgcacctcga ctaggcggct tagctgcaac aggaattttt tcttggcggc
     3241 atcggattct gtgaggatat ggtgatggtg ataaaacaaa ttaaacgcct gcgcgagttg
     3301 gaaggcgtat ttggcgacga aggccggctc ttgcgcattt accgaggcct cgatttgcgt
     3361 gtcgagcgag gccgccagga acgcaagttc ccagaacgcg tttccttcgg gaccggcgaa
     3421 taattccgcg gctgcgattg cgtccacatt ttccagcgat gagagatcga agtcgggctg
     3481 ctgttcgcga agtttgcgaa aaatgttgcg cgcgcgaacc acggcgtact ggcaataggg
     3541 gccggtttcg ccttcaaagc tgagagcatc tttgaagtcg aaggcgatgg tggccgtgcg
     3601 tgtaaatttc aaaaggaaat atcgcagagc tccgatggcg atggagtggg caatagcagc
     3661 gcgctccata tccggcgtac ccggatggcg ttcatcgact tcagcccgcg cggcggcttc
     3721 gaggcgatcg agtaaatcgt ccgccttcac acccatgcct ttccggccac tgacctccac
     3781 atacggcttt tttgcgtctt cctcgctcag agagtagccg agttcggcgg cacaacgcgg
     3841 cgtgagcgca acaatttcgt aggagaagtg aatcgagcgc gcagcctgat cttcaaagcc
     3901 aagcgcacgc agtcctgcgg ttacaacgtt ctgaagatac gcctgccgcg catcgatcac
     3961 gttatagacg atggcggcgc ggccaaacgc cggcgcggaa gaatctcctg ccgtggcagt
     4021 gctggaccac aacgtgtgtc ccgcgggata tctataaaat ttctcgtaat gaaaatcgcg
     4081 tcccagtaat ccaaatttcc aaagctgata ggcaatgtct tttcccacgt acgtaacggt
     4141 gccgttggag cgcacgatta ctttggcgtc gtcgtcttcg acggcaggtt cggccgatgc
     4201 attactcgct gtgatgccag gcgggtcact gctcggttca tcggcgtcag agccatcgtt
     4261 cattcgcatc acccagcagc cggaattttt tcccacggta gccaggtgaa tcgcgttgcg
     4321 cttttttaat aaatcgaatg cggcgtccca gaatttcaga tgtagaattt cactttcgcg
     4381 gggtagcagg tcgtattcaa tttccaggcg ttccatagtg cgcaagtggc agcgcacgat
     4441 cgccggtgcg acaatgcctg ccatctccga agcttcgccc gcaccttttt caatcgattt
     4501 cagggtttcg ccgcgcaggt tgaggcgtgt tttgtcttcg tcaaaaaaac gcgagacgct
     4561 cgcgtacaaa tcccagcagt agtaatcgaa tttcggttcg gcggcgagtg cgcgcacttc
     4621 cgccgctgat tttttcgcaa tatgcagaaa tccgatcacc acgtcggcga cctgcacccc
     4681 tgtgttatcg atgtaatttt gtacttccac gcactgcccg gcgcggcgca gcaggcgcac
     4741 aaacgtgtcg cccagcacgg cattgcgtaa atgcccaatg tgcgcggctt tgttgggatt
     4801 gatgttggtg tgttcgacga tgcatttcgg cgcatcaggc gcgaagccgg gacgccgcgt
     4861 gtcggcgtcc tttaacgagc tagccaaaaa cgccgtgcgt gaaaaataaa aattcacgta
     4921 gcccgcaccg gctacttcca ctcgcgcaac tccctcgatg ggcggaagct gcactgcaat
     4981 ttcttgcgca atttgtcgag gagcgcgctt cagacgcttg gccagctcaa agcaaacagg
     5041 cgtagcggct tcgcccatct cgatgcgggg aggtttctcc gtgacgatgg tcacgtccag
     5101 cttgtaatgc cttcgaatat gcgccgcgag cgcggcgcga acgcgtgcta cgagaacgtt
     5161 gaccaagtga ctcccatctg aatagagaaa cagcaattat agggaatgct cgacgaaagt
     5221 tgaaggagga ttgtgggggc gtcggcgagg gcaaaacgct aaaagcttta acacagaggg
     5281 cacagagaaa aaaggagaaa ccccgagaaa tgcgagcaca acttaacttg accaagcaaa
     5341 aattcacgat cgagattcaa aatcactcga ccacaatgtc tggcacctgt ccggttttct
     5401 gctcgtctct ctgtgcactc tatgttaagg ctttccgatt tgtgttatcg ctaactgaag
     5461 ccgccgcgcg tcttatgaat ttaattccca gcttgtctcc ccgtgtaagt cacgcgccaa
     5521 acactgttcg acgcatcatc ggtaaacaca agtgcgccat cgctggcaac ggttacgccc
     5581 acgggacgtc cccaaacatt cgcgtcgtcc acgacgaatc cagtgacgaa atcctggtac
     5641 tcgcccgtag ctttgccgtt cggcgtataa acgcggatga ctttgtatcc tgtgcgcttg
     5701 gcgcgattcc acgagccgtg cgaagtgacg aacaaatcgc cgcgatattc agcagggaat
     5761 tgcttgcctt cgtaaaacgt aagcgtcaaa ggagcggagt gggactgcag caacacatca
     5821 ggaacaagaa ccttctcttt cagttcaggg tgctttcccg caaaacgtgg gtcctggttc
     5881 ggccctgtgt aataccacgg ccagccataa aatccgcctt cttggatgtg cgtgacgtag
     5941 tccggcacaa gattgtcgcc gagcgcgtcg cgctcgttta ctgccaccca gagttgccct
     6001 gtttgcggat gcatggtcaa gccggatgca tttcgaacgc cggccgcgta aatgtgctgc
     6061 ccactcccgt cgggattaaa ctcgagcacg tcggcgcgat tggtttccga ttgatcgtca
     6121 cccacattgg ccttggaacc gatgccaaca aagagttttt tgccatcatt cgaaaacacc
     6181 aggctgcgtg tccagtgacc tccgccgtat ctcgcgccgc ccggaatgct tgcaacaatc
     6241 acttgcgccg ggccgctggc cttcaggtca ccgttcttgt aagggaagcg aacgatcgag
     6301 ttcgtgttgc cgatgtagac gaattgcgga ttcgggcccg gaggataaaa cgcgattccg
     6361 tagggcatgg tcaggtccgt cgcaaagacg tcgttggtta caactttgcc gtcgcttcca
     6421 aatccacgta ggacgcgaat acggccaggt gtgctttccg cgacaaaaag atcgccgttg
     6481 ggggcggtga taattgtgcg cggagactcg aggcctgtca gcgcctggtc aatcttgaag
     6541 ccatccggca ccttcggcca ggcatcggcg ggacgcggaa caactttgga ttgattgggc
     6601 gccgaatcag tcgcgtaagg ctttggcaaa tctgaagcct tgatggtgta ccggttgccg
     6661 ggacgctcat tattccagtc gttgaacgag ccttcaccgc gcaggacttg cggcgcgctg
     6721 gctgagtttt gcgctcgcgc tgtcgtcgag aacatcaggc ccactgccat gggtgcaatc
     6781 cagagtgcgg ccggaatccg tcgcaaaaat ttacttggca tgatcattcg cctcctgata
     6841 agaaaccagg tgattcgaaa tttgttccag ttgaatacgt ttcttcgtct ttctgcatct
     6901 tagcaggcgt gcgacgcgga ttccagccga gggtttttgt gactagagtc gaaaatttgg
     6961 gagaactcaa ggatgcccgg ctaacccggt cgaagtgcgg gcggccaata gaaaaccgaa
     7021 tcgaacgacg caagagtaac agctgaaaat gcgttgcagc gtgaagatta cattaggaat
     7081 atagtagggc ggcagcatcc agaacagtac cgtccattgc tcgcgtagca agaaagtgga
     7141 actcctcctg ccaaaaacag caatagataa tgaagacgcg ggagagacgt ccaaaccggg
     7201 gtagatggca gagccagttg agacacgcga tatcgaactt ttggggcgtc tgaaagcggg
     7261 agatgaggac gcttttttag agttctaccg gcgccatcag ggcggattgt ataggtacgc
     7321 catgcatatg agtggaagcc cacaagctgc cgcggacgtc gttcaggaaa cgttcctttc
     7381 gttgatacgc cacgccggaa aatatgatca ggaaaaaggg aatccagtcg cttttctttt
     7441 tggcattgcg agaaatcatt tgcgaaagtt gtatgaaaaa gagagccgct atattccttt
     7501 atccgacgaa ttgagcagcg gcctctcagc cgatggagca aaccattcac gccattccaa
     7561 cggcaatggg cattcaccgg tcatcatggg acagtcagaa acgatccaag agggcttgga
     7621 gcgcgagcaa attggagagc ttttgcgtga tgccattttg actcttccgg atcattatcg
     7681 tgagcctgta acgctgtgtg acctggaggg aaagagttac ggcgaagcgg cagcgctgct
     7741 cgattgtccc gtaggaacgg tgaggtcgcg gttgaaccgg gcgcggtcga tgctgctgga
     7801 taagcttcgt ccggcgaaaa ttggggtgag agcgcaatgg gcgcgccccg gaggcaaggc
     7861 atgaaggaaa tgacgtgcag cgggtttgac gaggtcgttc acggcttcgt gcacatggaa
     7921 ttgctggatg tgactgtgcg tgagtccgcg ctggagcatg cggcccactg cgcgctgtgc
     7981 tcggaacgaa tggcggatgc gacggcactt gcggaagctt cggaagcgat gagtcaaaat
     8041 gcgcgcgaca agcaagcccc tccttatgtg gaggcggccc tgcttgttgc atttcgcgga
     8101 caccatcgac gtgcttcctg gcaacacacc ttggaatggg cgagcgtggg cgctgcggct
     8161 gctgtgctga ttctttttct gtggttaggc aacggaaagc aaaaaatgca atcggctcct
     8221 gggcctcgga aagatgcttc atcgcaatct accgtgcccg tattggctgc cgtgtcagag
     8281 cctttgaaaa tggatagtgc gccggcaaca actaccgcga aagccgcttc ggcggatact
     8341 tcaaagaacg aaacttatct ggcatcggat tttgttccag tgccgtacac tggcccgatt
     8401 gtggcggatg atccaggcat ggtggtgcga gttcagttga ccagagcttc cctggcgcag
     8461 cttggatatc ccgtcgccga gactccggac gaagatttga tccttgcgga tgttctggtt
     8521 ggtgaagatg gctggccgcg cggtgtgaaa ttaattcagt aatagttgtc ctttgaagca
     8581 ggaccaaggg caaatgatgt ttgaggaggg cacgtccgtg agaaaagtgg ggctaaggtt
     8641 aagcgcaggg gcaatgctgc tgggtggaat ggctatggtc gtttccgcgc agggcccgcc
     8701 tcctcccggc ggattcggcg gcggaccgaa gccggggata ttcgaaatcg gcggcttgat
     8761 gggcggattc ggtggcaagg tggtgaccgg gaaacccatt ctcgctacca ttacgatcac
     8821 gcacacagaa acgctgcctg gaaatttgat ttcgaatact tccactggaa cgtatgcgcg
     8881 gggagcggac ggtagcacgt atcgtgacgt gaaattttct gaaattggtc cgtgggcggc
     8941 ttcgggtaaa gcgaaagagt ttgcctacat ccggaatgta acaaccggca tggagtatat
     9001 cgttcatgta acagaaggca cgtaccaggc gtttccactg cgcgaacgca atgcatcgcc
     9061 gggatcgaaa ttcggtcctc cgggcggccc gaatcccaac gatgaaaacg tgacagataa
     9121 tcccaacgga tcttacacgg accctgccac gagtacggta taccctgcgg atgataagca
     9181 ggtaacgcga acgattccgg caggggccat cgggaacacg aatgccatag ttattacttc
     9241 ggagcgctgg ttttccaatg cctttgagat cgtttttgag gatacccata ccgatccgcg
     9301 ttttgggacg acgacgtaca agctgtcgaa tatcgggcag tcggcctcaa cgtcgctctt
     9361 cactcccaat ccgactttta ctcaagttca gggcggcgga ttcggaaagc gtggacccca
     9421 tggtccagga aagcagccgc ctccaccgcc acaggattga gaaacaatcg cgtggcgatg
     9481 ataacgcggg gaattacaaa tttagaaagg gcgccctgag ttgggcgcct tttttgatgg
     9541 acatggttgt ttgaattttg gcaaacaaaa attgcccgct caaatgcgag cgtccacgac
     9601 aaaagcaaca acgtagcggt tttgtagagt cttctccgtc ttatgttttt cgggcggaaa
     9661 gggcactttg cctcgcaaag tgtggcgttg caaaccaacg tgaggtcttc gcgaggattt
     9721 gacagcattc ggttgcccgc ttactatcga aattcgagtt ttcgttgcgg agcttgctgc
     9781 tgttcgacag cggcctgcca tcgtaagatt cactacacat tcaagctgca ggctagcctt
     9841 gcacggtcaa ggcaagaagg aatagcccgc agatttcacg cggaaagggc gaatgagcac
     9901 tccccgagcc aagccaaagt actacctgac gacgccgctg tattacacga acggcatgcc
     9961 gcacatcggg catacgtatt cgacgatcgt tacggatgtg atccggcgtt acaagcgcat
    10021 gcggggattc gacgtgttca tgacgaccgg cgcggatgag catggcgtaa acgtcgagcg
    10081 gtccgcgcaa aaagcgggga aatcgccact ggatttttcc acggaaatcg ctgtcgagtg
    10141 gaaacaactt tgggatgatc tcggaattgc gggcgacgaa tttattcgca ccacggacga
    10201 gaagcaccat cgcaccgtgc agtggctctt taagcgttgc cgtgacaacg gtttcatcga
    10261 caagggaagc tacacgggac agtactgcgt gcatgataac gcatacgttg tggaagcagg
    10321 acctggcgat ccttgtcccg attgcgggcg gccgacggag actgttacgg aagaaaatta
    10381 ttttttcaaa ctttctgaat ttcaacaaaa actcctcgat ttttacgcag ccaatccgga
    10441 tttcattcag ccagaaagcc gccgcaatga agttttgagt tttgtaaacg gcgggttgaa
    10501 aaatctttcc ataacgcgca caacaataaa atggggcatt ccggtgccag gcgaatttcc
    10561 gcacgttttt tatgtgtggt ttgacgcgtt gatcggatac ctgagcgcgg tgggtggtcc
    10621 tgactatgac cagtgcggtc tgtggcctgc ggatttgcac atcgtgggca aggaaattat
    10681 tcgttttcat gcggtgtatt ggccggcttt cctgatggcg gcggatttgc cgttgcccaa
    10741 acaaatttgg gcgcatggat ggctgttatt tgacagtcag aagatgagca aatcgcgcgg
    10801 caacgtggtg cgaccgcgtc ccatcgtaaa tgctctgggc attgaaccgc tgcgctatta
    10861 catgctgcgc gaaattgtgt ttgggcagga cggaagtttc tcgtatgacg cgatggtcac
    10921 gcgctacaac agcgacctgg caaacggatt ggggaatctg gccagccgca cgctgacgat
    10981 gatcgagcgt tattttggcg gcgagattcc aaagccggcg gttacgcagg gaagcgatat
    11041 gtacgttccg gccgcggcag attttgcggc ggaagcgctg acgaatatta caaagttgta
    11101 tgaccggttc agtttttcgc aggcgctcga agcgatttgg gagcaagttg ctgccatcga
    11161 taaatttatc accgtggaga agccctgggc tcttgccgat aagcctgaaa atcgtgatcg
    11221 cctggcgatg gttttgtact cggcggcgga aagcctgcga attattgccg cgctggcgca
    11281 ccctgttctt ccagaggcga ctcgaaaaat ttggaatcaa ttgggactta ctgagccgct
    11341 cgggcaagcg caactggatc aaattgcgtg ggggcagctc aagcccggaa ccaagattgg
    11401 gaagccggaa ggaatttttc cgcgcgtaga caaatccgaa acgattgcga ggattgaagc
    11461 catggaacaa gaaccaacga aagtgccgga gacggctaca gcaccggccg cgacaccgac
    11521 gactgcggcg gctacagctc cggcgactac tcctgcggca gctgcggctg ctccgatagt
    11581 agccaagatt ggcatcgagg attttgcgaa agtggaaatg cgcgtcggct tggtcaaatc
    11641 cgccgaacga gtggcgggcg cggacaagtt gctgcagctg caagtggaca ttggcgacga
    11701 agtccgccag atcgttgcgg ggttagcact ggcctataaa ccggaagacc tcgtagggcg
    11761 caaagtcgtg gtggttacga atttgcagcc gcgaaaactg cgcggcgtag agtcaaacgg
    11821 aatgatcgta gcggcatcgt tgccggacgg cgcgccagtg cttgcaggtt tcatcgaaga
    11881 tgtccccgtt ggggcgaagc tgaaataatg catggcagct tgttttttct aaaaggattg
    11941 ctgcttgggt tttccatagc ggcgccggtg ggaccgatcg gcctcctatg tattcagcga
    12001 accatcgcgt atggccgcag ctcgggattg gcaaccggac tcggtgccgc gactgcggat
    12061 ggagtgtacg ggacggttgc cgccttcggt ttaacagcca tttcgggttt tttggttggc
    12121 cagcaatttt ggttcagact aattggcggt attttccttt tctatctggg tgccaagacg
    12181 ttgttttcca gagctgcgga gaaagcggca tcgtcaaatc acaacagttt gttttcggac
    12241 tatgcttcaa ccgtttttct aaccatcacg aatccaatga ccattctctc ttttgccgca
    12301 gtcttcgggg cctcggtctt gccgacacag gcggacacgg gtctgccgtg ttgatggttg
    12361 caggagttgt tctcggctcg gcactatggt ggttcgcatt gagcggcggt gtttcgctgc
    12421 tcgggcccaa attaaatcga ggctccttga gtatcattag cacggtatcg ggcgcggtct
    12481 tggttggctt tggaattttc gcgctggcaa gcataccgaa aactggcaag tgattgcgtg
    12541 gcctagtatg cgattgttat tccgacatcc ggaaacgcga ttttcttgaa tagcgtcatg
    12601 cgcaacgcca atcgggagat tggcgttccc gcagatgaag caatgcggtt tgtggcaggt
    12661 tcttaaaata tggatttgat cgactcacat gcgcacatcg acgtttccga ctttgacgcg
    12721 gatcgcgggg ccatgctcga tcgagcgcgc gctgcgggcg tgggaacggt cctggctatc
    12781 ggcaacgggc cggagctgga aaagttaggc gcggctattc cctacgccga ggccaacgat
    12841 tggatttatg cggcggccgg aattcatccg catgaggcca agcaggctac cgacgcacat
    12901 tattcagaat tggaacgaat cgctcgaaat ccccgcctca tagcctgggg agagattggc
    12961 ctcgattatc actatgacca ttcgccgtgg gacgtgcagg caaacgtatt ccgtcatcag
    13021 ttgagccagg cccgcgcagc gaagaaaccg atcatcatcc attgtcgcga agcctggccc
    13081 gactgcctga atatactgga cgaagactgg cgaagttccg gcctgggggg tatattccat
    13141 tgcttcatgg gaactctgga cgaagcgcgt cgcgggatgg acatgggttt tatgatttcg
    13201 tttgccggaa atgtgactta cccgaaggca cagaatctgc gtgacgtggc gaacgaaatt
    13261 gcgctcgacc gtttgctcgt ggaaaccgat tcaccgtttc ttgcgcccca atctcaccga
    13321 ggcagaagaa acgagcccgc gtacgtcgcg gaagtagcgc agaccatcgg gaatgtgaga
    13381 aacttgccag cggaccaaat agccgaaatt acttctgcca actttcggcg gttctttggt
    13441 ttgggcgaat ccggaaatag gaataaggca gtccctggag gggcgtcatc tcacttctga
    13501 cggcgaagga aattttcgat ctcgtgcgcg acgaccttgt gaaggtcgag caggagcttg
    13561 cgctccagaa tggctcagcg attgagcctg tggcggaaat ttcgcgttac ttgctggagg
    13621 gcggcggcaa gcgcctccgg cccgcgctgt tgctgctttc cgcgggatat gccggctacc
    13681 ggggagagag cgcgattcgg ctgggtgcag tcgtcgaact gattcatagc gctacgctga
    13741 ttcacgatga cgtgattgac ggcgccaaca cgcgccgagg gcgcccttct gcaaattccc
    13801 gttggggcaa tcaccgcagc gtactggccg gggactggct ctacatgcag tctttccaga
    13861 tggcgctcac ggaacgaaat tttaaaattc tggacgtttt gatcgaactc acgcaaaaca
    13921 tggtggaagg cgagcttgtg cagcttgcca agctcgggcg aatggatttg accgagcagg
    13981 acgccgtgga gcttgcggcg cgcaagacgg cgtgcttgtt tgccggatgc gccagacttg
    14041 gcgcagtgct gggcaatctg agcgacactg gcgagcaagc catggccgat tacgggcatt
    14101 acatggggct ggcgttccaa cttgtggatg atgtgctgga tttcacagcg tctcctgaga
    14161 agctgggcaa gcctgtgctg agcgatttga aagaaggcaa ggtgacgctg ccgctgatct
    14221 acgcgcttga aaccgatggc gaagaaggaa agcgcctggt gcgaaccgtg ctcgaagaaa
    14281 aagggtttga tagcgttcga ccggaagaga ttactgaact cgtccacaaa tcaggcgcgc
    14341 tcgatcgcac gcgggctgcg gcctttgatt tcgcgtctcg cgcaaaggcc tgcttgaacg
    14401 ggaacgcgac atcggattat gggcgcgctc tcagaacagt tccggatttc attctcgacc
    14461 gggacaatta acaactgggt ttcactgctg attttcccat gctgccgttc aagctcattt
    14521 atcatcctca atacgatttg gatttaggca cacacgtttt cccctcgcag aaattcaggc
    14581 tcattcatga agcgctgcgt gccgaaggga tcgccgaagc cagcgatttt ctgcggccag
    14641 agcctgcgcc gaacgaagac attttgcgtg tccacacgca agcctatgta aataagctgc
    14701 tcacggattc gctcacactg agcgaacgaa tgaaattgga aatcccgctg acagaactaa
    14761 cgcgaaatgg attttggctt gcagcgggag gatcaacact ggccgggcag aaggcgctcg
    14821 aagatggatt tgccgcgaac ctcggcgggg gattccatca cgcgtaccct gggcatggcg
    14881 aaggcttttg catgattcat gatgtggccg tcgcgatccg gtcgctgcaa gcggcaggga
    14941 agattcgaac ggcaatgacc gtggacacgg atgtccatca cgggaacggg acggcagcga
    15001 ttttcatgga ggatcccagc gtctttacgc tgtccatcca ccagcaaaat aattatccgg
    15061 agcccaaacc gccttcggac gaagacattg ggctcgcgga cgcgactcgc gatcatgaat
    15121 atctggacgc gctggagaaa ggccttctgc gctcgtttaa aaaagtgtcg ccggacctga
    15181 ttttttacgt tggtggtgcg gacccgtatc gtgaggatca gctcggcgga ttggcgctga
    15241 ccctcgaagg attgcagcag cgcgacaaac tggtgttcga tcatgtgcgg cggcggggga
    15301 ttcccgtggc gtcaacgctg gcgggagggt atgcgcggcg agtggcggac acggttcgaa
    15361 tccacgtgaa tacaattatt gcggcgcgcg atgttttgtt gcggcaaatt cccgccggta
    15421 gttgatatcc taatttaatg tcggcggttc gtttttgtgg cataggcttc agcctgaggg
    15481 tttgggcttt gcaccaacta gccccttagg cgtttaatcg ttgtaatccc aaaacccaca
    15541 ggatgaagcc tatgccaccg tgccacctcg aatttaaggc gctgcggaag ctttcgtgat
    15601 ggcagcgacg gccaaatccg tgaacccatc cggtgcaaaa ccttgtgtgc ggtaggcgat
    15661 tttcccggat ttatccagca ccagaaccgt gggaatggat tcgacgtgga aagcctgatt
    15721 gatgcgatct gcaaatacgg aagcaccgcc aaatttttgc tccttcaaat aaggggcgac
    15781 taaattttca tcctcgtcgg cgttaaccgt aagaaagacc acatcatcgc ggctcgcaaa
    15841 ttttgtgcgg acttcggcca gcattgattc catcgaattg caataggcac accaggtggt
    15901 ccagaaattg aggacgacaa cttttccatg cgactcggaa agttttacgg tgccgcttcc
    15961 atcgacgtgc cgcaaactga attgcaacgc atcgctgaca tctttgttgt aaaccggaac
    16021 gtcagctttt tccgctttga gggcttcgcg gctgcggtcg tatgctgaca gcatgatgtc
    16081 gcccaacccc gctgtggagg gatgggtgta gcgccagaga ttgcccatcc gtaagcggag
    16141 catttcgcga tccatgggcg gaccgtttga gtccgattgc tcctgcccgg ccagcatgaa
    16201 tgccgaggcg tactggcgga tggcttcgtc cgattgtttt tcttcttccg caatttcgcc
    16261 cagcgttaat gcggcttcgg cattgggtag catgcggaat cccacggtaa gatctttcac
    16321 agcgtcatca ttcttgtgca aatgacgctg cactcgccct cgcaatacat acaggtccac
    16381 caagaatttg ttgcggcccg attgccaatc ttccagcgtc atttgctgcg ggcgcgaatc
    16441 gggatcggcc ttcgagacgc ggtcgatcag gcgcgtgtcg taatcgatgg ctttgagctg
    16501 attcgcgtcg ccggacattt tttcgagcag cgttacggcc agataggcgg actggctgtc
    16561 ttccggttga atggcgatca ctttctccgc gtaatcgagc gcggcttgcg agttgtgcag
    16621 attcatttcc gattcgatga gcgcgcggta aattgcagcg cggcggggag aatccggata
    16681 gcgcgagaga tacgattcca aattcttgac gagggtagcg ggatcgtttc cggcttgccg
    16741 aacgagaatt tgcagttcgg cgtcttgatc tggagcggaa tcctgcggct ccgcaggagt
    16801 agtctcagct ttgggtttcg gtacggcctg cgagggcgcg gatgattttt tcgccggggc
    16861 ttttgccgga ctcgcgctgg agggagattg ctgagcgcgg gctattcccg cgaagctaaa
    16921 ggccagcaga caaagcagca accgcgttgg gtatcgcata ggcggcgatt gtaactcgga
    16981 ttcgaaagct tgcagcgaaa aacacaatag gccaagtagt ttgccttttg tagcgcccgc
    17041 cttcagtcgg gcattctgag gcgggccaca atgcccgcct gaaggcgggc gctataaaag
    17101 gcttaaaacc tgcgtcaatt gcgatctctg attaagggat gggcggtttg gagccttatt
    17161 tgaagtcgag gagaacgtcc actacgtggc gatttgcgtc acggcgaagc agatggccaa
    17221 attcattgta gtgaacgggt aattcgccaa aaacctcgcg gctgggtttt gcataaatta
    17281 tcatgtgcgc ttcgtaataa ttgttgtcaa aaacttgccc gggtgcaatc tgccatgcct
    17341 gccgcaatct tttttcggca tcgacggata atcccgtgat aatcatttcg cccatgcgat
    17401 attgcgggcc ttcggttatg gctacgtgat aggaaacctg atgcgcggtg tcatccagct
    17461 gcggctccgc atccagtttc agatccagat agccgcgccg catatattca aatgtaatgt
    17521 cccgccaaat attttctatt tttattccgt ctgccaggtc ccctggtttc agtttcacca
    17581 gggagtccaa agtggacgaa ggctgagcca tgttgccttg ccacgagaca cccttccacg
    17641 tgtaggccga gccctggcgc actggaattg agatatcaac ctcactcact ttggggtcgt
    17701 tgacgtccgg gaccaattgc gtttgcggcg ggccgatttg cgcgcgaaga aaacccttgg
    17761 tgatatagag cgggcggact tgctcatatt caaaaagctc cacggcgaaa agggaataag
    17821 gctggccctt gatgtccgga atgcgatctt tcaaacgctc ggagtcggca gcaatcggat
    17881 cgtcgatctt cagcgattga acgcgtagtg gaacaccttc aacacgaaac tgcatttcca
    17941 tgccgtcgcc cgcgggcacg ctaatgagct ggtgcgttac gcttccttta atatttttcg
    18001 atcccagaag attttcaatg acagcagtca tttgatcgat catggagccg ctgtccggag
    18061 cttcgcccgt aaacatgccg acctggcttc tgatcgcgtc gcctatctct gcgctggtga
    18121 accatggaaa attgtcgaac gaaattggat aggtgggtgc ttccgtgacc tggaattcaa
    18181 gatcgatcgc cgtgcccttt gaagtgaagc ggaaatttac cgcggaaaaa attccgagtg
    18241 ctgcaagaag gttcgtagcg tcctggattt gcgcggccgt gacaacgtcc ccatttttta
    18301 gcccactcgc agcgatcacc tggtcctgcg ggaatttttg caggcctgtt accttgatcg
    18361 aattaatcgt ggaactgggg gccggattgg cgggcggcgc ctgcgcgcgc accgcgactg
    18421 cggcaacaaa aaggaaatga aaggcgaggc acgcaagaag gtagcgcttc acgaccgtta
    18481 catcttacgc gatgagcgac gcgggacaga aatagaaatt gtcataggct tcatgttggg
    18541 gtcttgggtc tttgcaagga caaaaaatcc gcaggcttca gaagcccgcg ccacttaaaa
    18601 gctattcaac ggttacggat ttggccaggt tgcgcggctg gtctacgtcg cagccgcggc
    18661 gaacggcgat gtgataggcc agtagctgca ggggaatgac ttcgagcatt ggagcgaaca
    18721 actccattgt gggtggcacg acgatcacgt agtcagatgc ttcgcgggcg agatgatcgt
    18781 tttcggacac gacggaaatg acgatgccgt cgcgcgcttt tacttccttg atattcgaga
    18841 cacttttttc gtagcgcgtc atggattcgg gatcgttttc gtcgcgcgtc gcgagcatca
    18901 cgaccggaag gttttcgtca atgagtgcat tcgggccgtg cttcatctcg ccggctggat
    18961 atccttctgc gtgaatgtaa gagatttctt ttagtttcaa cgcgccttcc agggcgatgg
    19021 ggaagtgaat gccacgaccg aggtacagga aatttgtgtg gcggaagaac gttcgcgcga
    19081 gaccttcata aattcccgct tcgtcgcctt gcagaacggt ttccatcttg tgcggaacgc
    19141 gcatgacttc ctgtaacaaa agtttttcgc aatcctcggt tagcgtgccg cgcacctgtc
    19201 caaggtacat cgccaggagc accaaagccg taagctgtcc ggtaaatgcc ttcgtggatg
    19261 ccacaccgat ttccggtccc gcgtgagtca aaattgtgcc agcagcttcg cgcgtgatca
    19321 tcgagccgta cacattgcaa attgccagtg tttttgaccc tttttgcttg gcttcccgct
    19381 gcgctgcaag cgtgtcggcg gtttcgccgg attggctgat gcagatggtg agcgtttttg
    19441 aatcgacgat cggatcgcga taacggaact cgctgccgta gtccacttcg acaggaatct
    19501 tcgcgagctt ctcgatcatg aacttgccgg tctgcccggc gtgccagctt gtgccgcagg
    19561 ccacaatctt tactttcgta aaatcgcgga attcgcattc ttgaatgtcc atcttatcga
    19621 gaaaaacttt gcccgaatcc agtgccacgc tggcgagcag ggtgtcgcgg acggcgcgag
    19681 gctgctcgaa gatttccttc tgcatgaaat gcttgaagcc gcccttttcg gccatgatcg
    19741 ggtcccatgt gatgtgctgg acggcgcgct ggatcgtgtt gccgtcaagg tccatcacgc
    19801 ggacgccatc cggagtgagc acggcgatgt cgccgtccgc gaggaaaaac atgtcgcgcg
    19861 tgtgcgcgag aatcgcggga atgtcggacg caacgaaaaa ttcgttttgt cccaggccga
    19921 tcacggacgg tgggccggtg cgcgcggcga tgattttatt ggggtctgcc gcggacaaaa
    19981 tcgctatgga ataaacgccg gtgagccgac gcacagtatt gcggacggcg tcttccagcg
    20041 ggccgccttc cttcatgtat cgctcaacaa gatgcgcaat gatttccgtg tcggtttcgg
    20101 tgacgaactt gtggccttca ctctcgagtt cgcgcttcag gtccacgtaa ttttcaacga
    20161 tcccgttgtg gacgacgacg atatttcccg tgcagtcgcg gtgcggatgc gcgttttctt
    20221 ccgtagggcg gccatgcgtg gcccagcgag tgtgtcctat gccataggaa ccgtcgacag
    20281 gcaactggcg aatgacttct tccaggttgt gcaacttccc tggagcacgg cggatgtgta
    20341 ctttgccgtc cgaaccgacc acagcgatgc ctgcggagtc gtaaccccga tattcgaggc
    20401 ggcgcagtcc atctagaatc aggggaacaa cttttttaga gccgatgtat ccgacgattc
    20461 cacacacagg ttcatctccg cagatttagc tgggcgacag acgagaaggc ccgcacaaaa
    20521 aagataacac aagagaaatg cgtatttacg taagtgaagg aatgtgtttc ctgaagcgaa
    20581 caacctgtac ctatatgcag ggaaataagc cgagttttag tcccgccgcc tactccgacg
    20641 tatcagtcat ggtctgttga catgctttga gaggggcgaa attccttgcg gtacagacac
    20701 ttattccacg atttcgtagc ggaattcttc gatgacaggg ttggctagaa tctctttcgc
    20761 catgcgttcg acctgctttt cggcagattc gcgggccaca ccattcaagt cgagcacaaa
    20821 gaacttgccc tggcggacgt cggaaacttc gctaaaccca agagaagaaa gggcatgctg
    20881 tatcgcctgg ccttggggat caagtaccgt tcgctttggc attacccaga cgtgtgcttt
    20941 catggccgga attataccaa gtccagggcc gatatgcggg tatgaaattt ttcgagcatg
    21001 gcttgcttct cgttgggcgg aaggaaggaa tggacgaagc tggcttcggc tagttggaca
    21061 atttcgccgg gagtcatccc catgcgctgc gcttgcgcgt attcgttcgt tagagtggtc
    21121 tcgaacatcg cgggatcgtc gctcgacagc gtaaccggaa ggccacgccg aacgaacagc
    21181 ggaagaggat gttgctcata tccggccgtg ggttgactga tttggcgtgc cagtgcgcct
    21241 gtgcgcaggt tgcttgtggg gcagacatcg agagggatat ttcgcgccgc gataaagtcc
    21301 atggtgcgct cgtcgcgcat gatcgaaata ccgtggtcaa tgcggtccac gcccaataat
    21361 tcaacggatt cacggacaat ttccggtccg ccaatttctc ctgcgtgaat cagccggtgc
    21421 atgccctggc tggcgacgta atcgtaaacg ggaatcaggt cgtttgtcgg aaggccaagc
    21481 tcatcgccgc ctattccgta ggcaacgata tccggtgagc gcaattccgc ggcgattcgc
    21541 gcgacttcca tcgcgggcgc agcgccccac tggcgcaccg cgtcaaaaat atatttcaac
    21601 ttaattcccg caactttagc ggcagcatca cacaacgcgg caaagtttgc tgctgggtcc
    21661 tgattacggc gaaacatcac tccgatggac aaagtgactt ccgcgtataa aacgttttgc
    21721 cgctgcatgg attcggcaaa acgttgcgca atcagcgcgt aatcttcagg agtgcgcagg
    21781 aaggaagtga tccagatgaa attttgaata aactgatcga agtcgcccgg cgcatagcgc
    21841 ggcgcggttt cttcgggcgt gagagtgact ccgtgacgcg ctgctaattc aacgagagta
    21901 gacggttcaa tcgcgccttc caaatgtatg tgcaactctg ctttggggat ggaggttaaa
    21961 acctgctcgg aaatggatgt gttcattatt tctgggctcc cggtgaatcg tgcaagatgc
    22021 tgaggttccg gtaataggaa aagtagaaca caagaccgat tctccgccaa ataaagaatg
    22081 aatcttgccc cgaattgtaa atgcgggtgc tgattgattt tggattttct ccaaaatgac
    22141 atatcgtggc gggtttcgtg gtgctggcgt cccgccggcg gatttaaatt tcttagtgtt
    22201 cagaacaaaa ccgccggcga gacgccgacg ctacaaaact ctgcggcccg catttatggt
    22261 cttcacgcac aattaaatta acctcggctt aggtagtgcc tcaatttgcg agaaaagtcg
    22321 ccctctccgt cccgaagatc acgggacgga gaggccgcta caaaaccgca gcgcatagca
    22381 gttgcttttg tagaggccgt agcactgcaa aattcaaatt gatgcactac ctcggcttac
    22441 gaacccccgt ataaatcaca ttttgtaact tcagcttgcg ttgcaagggt gctcgtctat
    22501 gatggttttt ctttactttc catgcgtgaa acgaacggcg gccaattatt tcggtgacag
    22561 aatccaaatc cgcgggcaag cgtctcgccg gacacggcag attgattacc acgctgggcg
    22621 tgggcttcgg aatcgccgtc gccatcggta acacaatcgg cgcgggaatt gtgcgcgcgc
    22681 cgggcgtaat tgccaatttg ctgccctcca cgtggttgta cttcgctgcc tgggcagtgg
    22741 gcggactgta tatgtcgctg ggggcgttgc agtttgccga actgggcgcg ctcttgccca
    22801 aaagcggcgg acaatacaat ttttcgcggg ccgcgctcgg tgactatgca ggattcatcg
    22861 tcggctggag cgattggatt tccacggcag gtagttttgc cgccgtctcg attgtgtttg
    22921 ctgaatacgc gggtgatcta ttgcccttcg cgcacggccg cgtgcgcccg attgccgcgg
    22981 gagtgatttt gttgttcgcg ctgctgcaat ggaagagcat gaagattggc agcggcgtgc
    23041 aaaatttcac gacgattttg aaagctgtgc tttttctggc cgtggtcgcg gtttgttttt
    23101 tccatcaagc aacgcccgtg acggcccttg cttcgcgcgc tgcggtgccg gtgggttggc
    23161 cacttttcgt ttcgttcatt gtggcgctgc aggcggtgat ttacacgtat gacggctggt
    23221 acggcgtcgt gtatttcggt ggggaagtac acaatccggg gcgggagatt ccgcgcgcga
    23281 ttttcggcac cgtgatttcc gtgctggcga tttattttct ggtgaacgca gccatcgtgc
    23341 atctggtatc ggtgcgcgaa tttgccggca gcgattttgc gatgggcatc gccgcgcgcc
    23401 gcgtacttgg ggctgcgggc gataatttta ttcgtgtggt tgttgtggtt ggcctcttga
    23461 gcagcgtgaa cgccatggag atggccactt cccgcgcgct ctatgcaatg agtcgcgacg
    23521 gacttttctt tccgctcttc gcgcgtgtga actccggtgg tattcccgct ccgtccttgt
    23581 ggctttccac cgccgtaggc ctgtgcttcg cgatgttcag cttcgagcgc gtcgccgcga
    23641 ttctcgcctc attcttcgta gccgattacg ccctcacgtt cgtctcgctc ttcgtcctgc
    23701 gcaaacgcat gcccgacgcc gagcgcccgc accgcgcctg gggctatccg tggaccacat
    23761 cgatcgcgct gattgcctcg gtcgtgtttc taatcggcgc gctcgcctcc gaccgcgaaa
    23821 acacgccgtt gacttttctt gttctagccg cgagctaccc ggtataccgc gcgctcaagt
    23881 gggcggcgcg gcgaagcgct gagcgcacga cgctgacgta gtcggctacc cgcaaacgtt
    23941 tttgctcatc gatcgcatac atctttcctc ccggaataag cgaaattctt gatctttgtt
    24001 ttggctatac aggaattagc ttacgcaagg ggttgggaga gagatgtaac ggggctactg
    24061 acgcatctga cgagaattag aaatttcaaa tctcaaatta aaaaatcaca aatcacaaat
    24121 cacaaagaga catcgctggt ccgctaaaaa ccaactatgc ccagctgaga ttcgcgtact
    24181 gctcaacaag tttttttgtg ccgtgatccg cgaagcggac ggtgaatttg cggtcgggct
    24241 catcgccttc tactgcgacg attgtgccgg tgccgtaagt ggcgtggcgc acgcgttggc
    24301 cgatgagcgg gtttgagttt gctttgccgc cgcgcgttga aacgctcgac gagcgcgggc
    24361 ggctggccac ggcgcttgag gaccgcgacg gggagtcgac gcgcttgacg cggcgcgaaa
    24421 attcttcctg gctgtacgaa tactcgggat cgggttcgta gcggcgcgtc tcgcccgctt
    24481 cggcgagcga accggccgcg gtgtcgatca gctccgcggg aatttcctgc aggaagcgcg
    24541 acggcatgga gccggagagc cgctcggagc catacatgcg gcggtaaacg gcgcgcgtga
    24601 gcgtaagtgt cgtcttcgcg cgtgtcatcc cgacgtaaca caggcggcgc tcttcttcca
    24661 attcctcgtt atcattttgc gagcgactgt gcggaaaaat tccttcctcc atgcccgtga
    24721 ggaaaacgtg ctcgaattcg aggcctttgg cgctgtgcaa ggtcatcagc gtgatcaggg
    24781 cgtcttcgtc aaaattatcg gcgtcgctga cgagggcggc gtggtcgagg aagtcggtga
    24841 gggtttcgcc gcgctcggcg ccttcggcca tggcgttcac gagttcgcgc aggttgtcgg
    24901 cgcgcgccgt gtcctccacg ttgtcgcgtt gcaggagcat gtcaagataa ccgctgcgcg
    24961 tgaggatcga ttgcaggaaa tcggcgggcg tgctgctcgt aacttccttt tgcaattctt
    25021 cgatcaggat gcggaaatcg cgcagcggag caagagcgcg gcccgagaac gaagggtcga
    25081 gcgccttgcc aatggccgtc cacagcgaaa tgtcctgctc gcgcgcgagc gcggagaggg
    25141 aatcgatgga cgtttttcca atgccgcgcg gcggagtgtt gatcacgcga agcagggcga
    25201 tatcgtcgtc cggaaaaagc gcgaggcgcg cgtaggccag cgcatccttc acttccgcgc
    25261 gctggtagaa cgaaaagccg ccaagcatgc ggtagcgcat gccgcgcgaa cgcagggctt
    25321 cttcaaatga gcgcgattgc gcgttggtgc ggtagagaac ggcgatgtgg acggccggat
    25381 cgtcgccatg aagcatgcgc atgcgatcgg cgacatgctc ggcctcggct ttcgagtcgc
    25441 ggccctcaaa aaatgctaga tttcttccgg cggagcgcgt ggccttcaga gttttcccga
    25501 ggcggcggcg gttgttagag acgacgccag ccgcggcatc caggatgact tgcgtcgagc
    25561 ggtaattttc ttcgaggcgc acgacgcgcg cgccgggata atcttcttcg aagcgaagga
    25621 tgttgccgat atcggcgcca cgccagcgat agatggattg atcttcatct ccaacgacgc
    25681 acaaattgga tttttcgttg accagaagct ttaagagatc gtactggacg cggttcgtgt
    25741 cctgatattc gtcgacgtga atgtaacgga aacgcgattg ccatgcggcg cgcgcttcgg
    25801 gaaattcgcg cagcacttgc acggctcgaa gaagtaaatc gtcaaagtcg agcgcgccgg
    25861 ctttgcgaag aatgttttcg tactctccat acacttttgc ggcagtgatt ccgagaggat
    25921 cgccgcgacg ggcggcctcg gcggcaacct gcacgggagt ttgcgcatga ttttttgcgg
    25981 agctgatgcg ttcgagcagg ccgcgaattt tttcgccggg atccttgatg ttcaggtgat
    26041 cgagcgaaag ttttacggct gcgacctgat cgtccgcgtc aaaaattttg aagtcgcgtg
    26101 gaagtccgag gcgcttcgct tcgcggcgca gcagccacgc ggagaaagca tggaaagttc
    26161 cgatccacgg atcgtcaaac gcttttcctt ggcggatcag caaatcgcgc acgcgttcct
    26221 tcatctgcgc cgcggcttta ttggtaaacg tgacggcgag aatggaactg ccgggaacat
    26281 tttgttcgat gaggttcgcg atccggtacg tgatcgcgcg cgtcttgcca gtccccgcac
    26341 cggcaagaat cagcacaggc ccttctgtag tggtagcagc ttcgcgctgg ccggaattca
    26401 attcgtctaa gagtgtcatt tgggggagtg ggcccgtttg ggagtcaaca ttctatccga
    26461 cacgcaagcg ttcggcaaat cagcgcgtga cgataaaatt atcgttgagg cgcttcagct
    26521 agatcgatct cgaaaaagaa cagggctttc ttgtcggtca tgaaggcgag gtcgggaatg
    26581 tagagatggg agtcgtgaat tgcatcgaat tggtggttca aatcgaaaaa gagaaaaccg
    26641 tgagtggttg catgtggcgg caaaacatct gtcccgagag ccacggcacg cagcgccgtg
    26701 gtcatctgca cccaatcctt gctgtgtccc gaaccggatc cggtgccggg gaacggaaat
    26761 gggcgatgct cgcgaatatt cgattcggct ttcaggagcg tgcgatcggc cacatcttcc
    26821 ggagaaagag ggcccaaacg ttggcgatca tggtccggct gcgaaataat cagctcgatc
    26881 gtatcgccgt tcagtcgaat cggtgcgctg ctatcgttct tgaaatacac gtcgatggca
    26941 ataattccgg cattgtagaa agaatctttg ccgaaaattt cttttttgta gcggtcgacc
    27001 ttgaggtacg gatccgcgat gatcgtaagg tcttggtgcg catcgcgggt gtgcagcgac
    27061 atcgggtccg ccggcgcgct ttgcgccaag gcacgaacgg gaagaagcat cacaaatatc
    27121 aggattcctc gaagcatggt ctggctggct atgatgcgcc tttcccggaa taggttcgct
    27181 taggtttcgt agaggggaca gcctcggcca tgattaaagg gcagctgcgc gactccctgc
    27241 tccactaaag gtatgccaac gccgacggtg cgcctataat atctgttttg taagtgcgac
    27301 aggtatattt ccgcaaactt tgcggacagg agatggctcg ccgagagcct taccggatgt
    27361 atgcgattta tagtctgctg acggccgcgg gaatgctcct gctctctccg tatttcctgc
    27421 tgcgcgggct cgtccagaag aaatatctga gcaatcttcc tgaacgcttt gggcgaaagt
    27481 ttccgtcgga attgcttgct ggaaacacgc caggttcaaa agaaaaatcg atctggatcc
    27541 atgcggtatc tgtaggagag gttcttgctg tacttccgtt agcacagcga ctcaagaaaa
    27601 agtatccaca gcgccgcctt gtcgtttcca caacgactct tgcggggcaa acactggcgc
    27661 gagaaagatt acttttcgcg gatgcaattt tcttttttcc tttagactgg cgcggccctg
    27721 tacggcgagc ccttgcggca gccaatcctg cggcagtgat gattgtagag acggagatct
    27781 ggccaaattt tttgcgcgag tgccgtcgcg ctaatgttcc ggtggcgttt gtaaatgggc
    27841 ggttatcgga gcggtcgttc cgtggattta agcgcgcgct ttcatattcc gcagggatgc
    27901 tctctggatt tttgaaaaag gtgctggacg atgcaacgct ttttctgatg cagagcaaac
    27961 aggatgcggc gcgtctagtc gcattgggtg cgccccctga ccgcgtcacg gtaactggaa
    28021 atctgaaata cgatctcgca gaacctgatg caagtccggt ttccgcgtgg cttgaggtcg
    28081 aaattgcacg cagtgaccgc cgcccggtga tcgtggcggg aagcatattg gccaatgaag
    28141 agacgcctgt gttgcaggca tttgcggaag tcgagggcga gtttccgcga gcgctgttga
    28201 ttctagcgcc acggaagcca gagcaattcg ataacgctgc gacaatcgtc tgcgaatcag
    28261 gtcgaagact gttacgccgc cgcgagcttt cgctgaatgg tgttggaagc tcgatcctgg
    28321 ctgatccggg taatgtgcta ttgctggaca gcatcggcga gctagccggg ttgtatcgcc
    28381 tggccgatgc cgtatttgtg ggtggctcac tcgtgcccag cggcggacac aatatccttg
    28441 agccggcggc cttcggcaaa gttcccatct atggaccatc catggaaaac ttccgcgaaa
    28501 tggcgggaaa atttcttgcg gcaggcgcgg caatccaggt gagaaatccg aaggaattgg
    28561 gtgcggcgtg gcgaactctg ctgagagatt cacaaaccgc aaatcgcatg ggtacgttgg
    28621 cacgtgagct tgtcgatctg aatcgcggcg cgaccgatcg agtcctgggg caattagagc
    28681 acctacttgc cgatgagcgg agcctgaagt gaagcacgct tctccatggc tgcgccttct
    28741 gtatccctta tcgcttattt attcagcgat tgtgcgcgct aaaagctggt gctacgcacg
    28801 aggaatattt cgcggacgca aattgccggg aaccgtgatc agcgtcggaa atctcacggt
    28861 aggcggcacg gggaaaacgc cgatggtgct ggcgattgcg gagcggcttg cggaggaagg
    28921 gaagcacgcg tccattttga cgcgaggtta tcgaggcaca aaagccacag atgaaactgg
    28981 cattccgcag agcgacgaag ttgcgctgct gcgcgaacgg cttgcgggga aggtccaact
    29041 tggcgttggg gcggaccgtt acaagaatgg tttggtcctg tcgaaacacg gcgtggattg
    29101 gtttgtgctc gacgatggat ttcagcatcg gaaacttgtg cggaacgcgg atgtcgtgct
    29161 cgtggacgca atggacccat ttggcgggaa aatggttttg cccgccggcc gcttgcgcga
    29221 gccgctctcg gcaatgaaga gggccgacct tgtagtgatt actcgaagtg tgcaaacgcc
    29281 ggcgcctgct ctcgaggcga tgcttcgacg gcacacgaat cgcccgattt tttatgcttc
    29341 cacgcgactg ctgaacgtat cacgtattcc ggggatggat gtttccttgc cggaagaagt
    29401 ttggcgaaaa gcacggttcc tggcattttg cggaattggc aatcccgcag catttttcgg
    29461 tgatttgcgc acttggagct tgcaggcggt tcaggagcgc agctttgcgg atcatcattt
    29521 gtacactcca gaggaagcgg cggagttgga gcaggctgcg ataagttccg gcgccgacgc
    29581 cttgctgtgc acggaaaaag atgtatggaa tttgcggaac gtaaagttcg agaaacttcc
    29641 cgtctactgt tgccagatat cgtttgagtt gccggaaaac ttttggagcg ccgtttcgga
    29701 agcagtacat cgcgggaagg cgggcgagac gcaatgagaa ttcttgtgcg cgccccgaat
    29761 tgggtgggtg atgcggtgat ggctgttccc gcgctggaag cggtccgcga tacacatgcc
    29821 aacgatgaaa tttacgtgct cgcgcggccc gcggtcgctg atttgttcgc ggggcaaccg
    29881 tttgccgaca aagttgcgct atacgatttt cgtggcaagc atgcaggttg gtggggcagg
    29941 gaaaaactta tcgcggattt gagaaatgaa aaattcgatg tcgccattct tttgcaaaat
    30001 gcttttgatg ccgcatggct gacgtggcgg tctggaattt ccgagcgtat cggatatgcg
    30061 cgagacggcc gcaggccgct gcttacgaag gcgattcgag ttccggcaca aggcgaaata
    30121 ccaaggcacg aatcgttcta ctatttggaa ttgctgcggc gtgcaggatg gattgagaca
    30181 cggtcggaga ttgcgccgat tcgcttgcag atttcagagg agtcgcgctc atcagcggaa
    30241 tctatgttga gaaccgctgg cacaagggag aatgcgtggc gctgcgcaat cgctcccggc
    30301 gcttcctatg gcgcggcaaa atgctggccg ccggaatatt tcgcgcagct tgcggatcgc
    30361 ttgatttcgg aatgtgacgc cgatgtgata ttttttggaa caccgggcga aaaggaaatt
    30421 gctgctcgca taggcgccca tatgaagtcg cgcgcgattt cactcgtggg tgagacatcg
    30481 atgcgtgatc tggcggcgat gttcgcttcc tgctcggtgt ttatcggcaa cgattccggc
    30541 gcaatgcacg tggccgcggc tgcgggtctt ccggtaatcg gaatttttgg ctcgacggat
    30601 ccggagggca ccgcgcctgt gacgcaacaa tttacgttga ttcgagaagc tgtttcctgc
    30661 agtccatgtt ttttgcgacg ctgccccgtg gatcaccgct gcatgacgcg catccgcgtc
    30721 gattccgtat ttacggccgc catgcaaatt aaaaattctc ccaatcaggt atcgattcga
    30781 ggcgcgaaga atgcctgaaa agcagatttt gcgccgtgcc gtgtttcttg accgcgacgg
    30841 cacgatcagc gaagaaatgg gctatgtaaa tcacattgac cgctttcaaa tcttcccgta
    30901 tgcggcggaa gcaattcggc agttgaatca ggctgatatt cccgtcatcg ttgtaacaaa
    30961 ccagtccggt atcgcgcgga aaatttttcc cgaatcgctg ttgcatgaga ttcacaaaaa
    31021 gatggtcttg gaactcgcca aaggcggcgc gtggattgat gcgatttatt tttgtccgca
    31081 caaaaaggat gatgcgtgcg attgccgtaa accgaatccc ggcctgatgg agcgcgcggc
    31141 gcgcgagcat gggctggatt tgtccgcctc gtggattgtg ggagatcgct acggtgacct
    31201 tgaaatggga cacgcggcgg gagcgcgcgg aatcctggtg aaaaccggat acggccgcgg
    31261 agaatacgaa ttgcatcatg agaagtggcc caggcagccc gatgccctgg ctgagaatct
    31321 aagcgaagcg gtgcggctca ttctgcagaa tggcggcagc gcgccgcgtc gcaatgaata
    31381 agcacgaaga tcgctttctg gttgtacggc ttaccgcgct tggagatatt ctgcatacag
    31441 tgcctgcggt cgcggcgctg cgggctgctc acaagaacgc aaaaattgat tgggtcgttg
    31501 agcggaaatg ggcgccagtg ctggaaggaa gccctgcaat taacgaagtg attccatttg
    31561 accgccgttc cacatggggc gcgctcgagt gcgtggcgcg tttgcgcgag aatcggtaca
    31621 gctgcgcgat agattttcaa gggctttata agtcatccat ccttgcatcg ctttccggtg
    31681 cgtcgtgtcg cataggtttt gagcgtgctt gggcgcgcga agagggtgcg gccatgttgt
    31741 acacggagcg tgtcagtccg gtgggccgtc acgtcgcgga gttgaattat tccctcgcgc
    31801 agcgagcggg agcatcccac ccgcaaacgc cggaatatcc gctcagagtt ccggcaggcg
    31861 gtgcggcctc agttcgcgcg cgtctgcacg atcttggaat caccggtgat tacgtcgtgg
    31921 ttgggccggg cggaagttgg cgcgcgaaat gttggccctc ggaacgctat ggcgaatttt
    31981 gccgtgagtt agaaagaaaa tttggaatgc gcgttgtagt gattcacggt cccggagaaa
    32041 aacaaattgc ggaagaagta ttgcgcgccg ccgcgccggc acagcccacg cttatctcca
    32101 caacaattga ggaattaatg ggactacttg cacacgctcg atgtttgatc gcagcggatt
    32161 ccggcccgct gcatttggcc gcggcgctcg gcacgccagt tgtgggtttg tatggcccta
    32221 ccgatcccgc gcgcaacggg ccgttcgttt ctaacgtcgt aatcgtgcat aaggcgcgcc
    32281 ttgaggagat cagctacaag cggcgcgtgg aatattctcc cgcgatgctg cgaattacga
    32341 cggaagacgt gatggctgca acggagtcgc ttctgaaggt tcccgcttga gcaataaatt
    32401 gagcaacaaa actaattttt ggattcgctg gcgtgtacgt gtcggatacc cggttggaat
    32461 tgctgcattc tggttcgctc acccgcaagt aaagtggctg atttgcggtg tgagcatcgc
    32521 gattgtgggg ctaattattc gcggatatgc cgccgggcat cttcgcaaac accagcagct
    32581 agccacttcc gggccctacg catatactcg caacccttta tatttgggaa gcgtgcttct
    32641 tgccgcggga ttttccgttg ccggccactc atggatttcc actctgttgc tggcggcata
    32701 tcttgcaatt ttttatcctg ttgtaatccg gcgtgagcag acggaattgc aagcgcatta
    32761 tggcgatgca tttgtggagt atgcttcgat ggttcctgca ttttggccgc gactttcgcc
    32821 cgcaatggct tcctcagaga aattttcttg gccgctatat cttaaaaacc gggaatatga
    32881 agcgagcatc ggccttgcgg tggcgatgac cattctgctt gcgctgatgt tttggcgcgg
    32941 tgggatttaa ataatttcgt tgcgggttta tgtggcagag acttcagccc gtggagtttt
    33001 gtccttgcaa ggcctaaccc cacaggctga agcctgtgct acttaaatct gagatttatt
    33061 ttttggctgt agagaatgat tgcgatgcag ccgcgcggag aaatgtgctc acggttagtg
    33121 tgctgccatc agtgctcgca gtgattgctc cgtcgcggtc tgtccggtaa acgcggacac
    33181 ctgccgcgat cagccggtcc gtgacctcgg gtgatgggtg gccgaagctg ttctccctcc
    33241 caacggaaat tgcagcgaat gcaggatgcg ccgcggatag aaaagcatcc gtcgtggaag
    33301 tcttgctccc gtgatgtgca accttcagaa aatttactcc cactggctgc gcctccgcca
    33361 gaattgtgcg ctctgagggg cgttcaacat ccccggccag cagaaaagac tcagcgccgt
    33421 tgttcaaacg caaaactaga gaatcgtcgt ttttcgctga gcgaccctcg gcatcgtctt
    33481 ccggccatag gacgctgccc gacactccgt cttcggagaa attgtcgccc tgcttcaaat
    33541 gcttgatgat tacgccgtgc gccgttgcct gcgcgatgag ttgttcatac gctgcgctgt
    33601 gaatgtctcg cccaacccac agttcaccca cacgaaaatt ttcaaaaatg gcgggaagcc
    33661 cgccgaggtg gtcctcatgc gcgtgagtta gggcaacgac atcgatccgc ttgatcccgc
    33721 gcgacca
//
LOCUS       DASZMB010000229         4940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_222930, whole genome shotgun sequence.
ACCESSION   DASZMB010000229 DASZMB010000000
VERSION     DASZMB010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222930"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="VG272_10710"
     CDS             complement(<1..817)
                     /locus_tag="VG272_10710"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF14197.1"
                     /translation="MKNENDCACHPKVSRRKMLQGGLAAAAAVTSFSGMGESLLAETP
                     ASFPASEAKKLGARPIDIHAHYFPQEYFDLFNTEGQRYNTEFRMTEKGFTFKTPAESN
                     GPLPAKFIDIKQRLADMDAQGVAVHALSLTGPMVYWAEGEFSLKLSQTWNDGAVAAHQ
                     AHPDRFVVLAMIPMLDPDRAVEELNRVSKLPGVRGVYMGTNIEGRDLDDPLFDPIFTR
                     IESLNLPVFLHPLPPILGGKRLQPFSLTNLVAYPLDTTVAASHLIFGGVLDRHP"
     gene            1186..4071
                     /locus_tag="VG272_10715"
     CDS             1186..4071
                     /locus_tag="VG272_10715"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWF14198.1"
                     /translation="MKSALRNRLLILCLSVAVAAGSVFSQDAAKKAPPRRVSASQVST
                     AANVKNAEADLSIPTIKFEKYTLANGLVVILSEDHRLPLVSTNIWYHVGPANEIAGRT
                     GFAHLFEHMMFEGSKHVPGNAHIRFLEAAGASDLNGTTDFDRTNYFETLPSNQLELAL
                     WLESDRMGYLPDKLDQASLTNQQDVVRNERRQNIENSPYGIVEEDVFHLLFPKSHPYH
                     ADVMGSHADIQAAKLEDVRNFFKLYYAPNNASLAIVGDFDPAQAKQWVEKYFGPLKRG
                     APVPKIAAVTPPIATERRAVIHDQVELPRVYMAWLTPAIFKPGDSDADLASDILGGGK
                     SSRLYKKLVYDKQIALDVSASQQSLTLGSVFEIVVTARPGHTAAEMEKAVDEELAAFR
                     KNGPTQVELDRARNGIETEMIQGLERLGGFGGVADRLNQYNHYLGNPDYFRQDVHRYE
                     VETTDSVRTFAQEKLKPAARVVVYGIPGKPDLGPDVPTPKNLQKGQSTGGESVNADAA
                     WRENPPPAGPTHELNLPLPDIFKLSNGLTVYYHYRAGLPVAAANLVFRTGSGANPVDK
                     PGLASFTSNMLQQGTQARNATQIADEAALLGTTLSSNVSMDASSVGSSALTKNFPGVL
                     DLISDVVLHPTFPPDEVERRRASRLAALTDERSDPETVVARASVAALYGPQSPYGYDN
                     TGTEASNKAMMRDDMVNFWKTNYVPNNAALVVSGNIPVEDLKALAENKFGAWKAGEIP
                     QSNIGDLESTKAKIVIVDRPGAQQTMVRMLETGVDRATPDYPALEVMNSELGGLFSSR
                     INLNLREEHGYTYGAGSSFVYRRPLGYFAVGGGIRTDVTAPAVTEILKEIHRMMDTQL
                     KPDELSLAKDSQSRSLPGMFETDNGEAGALSEIFVYNLARDYFSTLPERLNAVTAEDA
                     EMVAKKYLHPDQLIVVCVGDRAKIEPELLKLDLGAVEIRDADGNVVK"
     gene            complement(4090..4494)
                     /locus_tag="VG272_10720"
     CDS             complement(4090..4494)
                     /locus_tag="VG272_10720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HWF14199.1"
                     /translation="MPEKIIRVLIAKPGLDGHDRGAKIIARALRDAGMEVIYTGLRQT
                     PEMIASAAVQEDVDVIGLSILSGAHNTLVPQLMDLLKQKGMNDVTVLLGGTIPEADIP
                     ALKKAGVAAIFLPGTSTQDIVDFVRQRAPRPV"
     gene            complement(4562..4891)
                     /locus_tag="VG272_10725"
     CDS             complement(4562..4891)
                     /locus_tag="VG272_10725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HWF14200.1"
                     /translation="MGAADSPYLSSQKIQLPSFQVPVLGYATASTLLPVCHLYNALIS
                     KLDCGYRLDLLVEDRIIVELKSVENLAPIHEAIILTYLRLSGHKTGLLINFNVAVLKD
                     GVRRFIV"
BASE COUNT         1139 a         1227 c         1459 g         1115 t
ORIGIN      
        1 tcggatggcg atcgagaact cctccgaaaa ttaagtgcga ggctgccacc gtggtgtcca
       61 gcggataggc aaccaagttc gtgagcgaga agggttgtaa acgtttgccg ccaagaatgg
      121 gcggcaaggg atgcagaaaa acgggaaggt tgagcgactc gatccgcgtg aagatcgggt
      181 cgaaaagagg atcgtccagg tcgcggccct cgatgttggt gcccatgtaa acgccgcgca
      241 cgccggggag tttgctgacg cggttgagtt cttccacggc gcggtcgggg tcgagcatgg
      301 gaatcatggc gaggacaacg aatcgatcag gatgcgcctg atgcgcggcc actgcgccgt
      361 cattccaggt ctgcgacaac tttagtgaga actcgccctc cgcccagtag accatcggcc
      421 cggtgagcga cagcgcgtgg acggccacac cttgcgcgtc catatcggcg aggcgctgtt
      481 ttatgtcgat aaattttgcg gggagcggcc cgttcgattc cgccggcgtt ttaaaagtga
      541 atcctttctc ggtcatccgg aattccgtgt tgtagcgctg gccctcggtg ttgaacagat
      601 cgaaatattc ctgcggaaag taatgcgcgt gaatgtcgat gggacgcgcg ccaagctttt
      661 ttgcctcgga agcagggaaa gacgctggag tttcagccag caaggattcg cccatgccgc
      721 tgaacgacgt tacggccgct gccgccgcga ggccgccttg gagcatcttt cggcgcgaca
      781 ctttaggatg gcaggcgcaa tcgttttcgt tcttcatggc atagtcctta attaggagcg
      841 aaaaatctcc gattggattg ggtattgatc ccgcgaatcg tacgctcttt ccgggagcgt
      901 tacaacgggt ctcgtggcat aggcttcagc ctgtggaatt ttggccttgc aaggagagaa
      961 gcagagtagg gatttcaatg gaacggcatc taagtggatc gaacagcact ttaagatttc
     1021 aatttgtgtc aggaccaacc ccccgcaggc tgaagcctac gccacggtgc ttaactttaa
     1081 ggtatcttag tatttgtcgt tctgtgtgct gcgatgcagc ggaatggctt agccctgcgt
     1141 cttatatctt atgcgtggtg gaaacattgt tgaggagact tcgggatgaa atccgcactg
     1201 cgtaaccgtt tgttgatttt atgcttgtcc gtggctgtcg cggcaggatc cgtttttagc
     1261 caggatgccg cgaagaaagc tccgcccaga agagtttctg cttcacaagt ctcgaccgcg
     1321 gcaaatgtta aaaatgcgga ggcggatctt tccatcccga caatcaagtt tgagaaatat
     1381 acgctggcga acggccttgt tgtaattctt tccgaggatc accggctacc gctggtctcc
     1441 accaatatct ggtatcacgt tgggcccgcg aacgaaattg cggggcgcac aggattcgct
     1501 cacttgttcg aacacatgat gtttgagggt tcgaagcatg ttcccggcaa tgcccatatc
     1561 cgttttctgg aggcggccgg cgcttccgat ctgaatggga ctaccgattt cgatcgcacc
     1621 aattatttcg aaacgctgcc ttcgaaccaa ctcgagcttg ccctgtggct ggagtccgat
     1681 cgcatgggat atctgcccga taagctggat caggcaagct tgacgaacca gcaggacgtt
     1741 gtacgcaatg agcgccgcca aaatatcgag aacagccctt acggaattgt ggaagaggac
     1801 gtgttccatc tgctttttcc caaaagccat ccctaccacg ccgatgtgat gggttcgcat
     1861 gcagacattc aggcggcgaa gttggaggat gtgcgtaact ttttcaagct gtattacgcg
     1921 ccgaataatg cgagcctggc gatcgtcgga gatttcgatc ccgcgcaggc caagcagtgg
     1981 gtagaaaaat attttggtcc gctgaagcgc ggtgctcccg tgccgaaaat tgccgccgtg
     2041 acgccgccaa ttgcaacgga acgccgcgca gtcattcatg accaggtgga actgccgcgc
     2101 gtgtacatgg cctggctcac gcccgctatc tttaaaccgg gagattcgga tgcggatctc
     2161 gcatcggaca tcctgggtgg agggaagtcg agccgtctgt ataagaaact tgtctacgac
     2221 aaacagattg ctctggacgt aagcgcgtcg cagcaatctt tgacactcgg ctcggtgttt
     2281 gaaattgtgg tcaccgctcg ccccggccac acggctgcgg agatggaaaa agccgttgat
     2341 gaggaattgg ctgcgtttcg caagaacggt cccacgcaag ttgagttaga ccgcgctaga
     2401 aacggaatcg aaaccgaaat gattcaaggg ctcgagcgcc tcggcggttt tggcggcgtg
     2461 gcggaccgat tgaatcagta caaccattat ctcgggaacc cggattattt ccgtcaggat
     2521 gtgcatcgct acgaggtgga gacaacggat tccgttcgca cttttgcgca agaaaaactg
     2581 aaacctgcgg cgcgcgtggt cgtgtatgga attccaggta aacccgatct ggggccggat
     2641 gtgccaacgc caaagaattt gcaaaaggga caaagcaccg gcggggaatc ggtgaacgcg
     2701 gatgcggcgt ggcgggaaaa tcctccacca gcggggccca cgcatgaatt aaatttgccg
     2761 ctgccggaca tttttaaact ttctaacgga ttgactgtgt attaccacta tcgcgccggg
     2821 cttccggtgg cggcggcgaa tcttgttttc agaactggca gtggggcgaa tccggttgat
     2881 aagccggggc tcgcgagttt cacttcaaac atgttgcagc agggaacgca ggcgcgaaac
     2941 gcgacacaaa tcgccgacga agccgcgctg ctgggaacca cactttcttc caatgtgagc
     3001 atggatgcat ccagcgtcgg atcgagcgcg ctcacgaaga attttcctgg agtgctcgat
     3061 ctgatttccg atgtcgtttt gcatccaacg tttccgccgg atgaagtcga gcggcgacgc
     3121 gcatcgcgac tcgctgcttt aaccgacgaa cggtccgatc cggaaacggt tgtcgcgcga
     3181 gcgagcgttg ctgcgctgta tggaccgcaa agtccgtatg ggtatgacaa tactggcacg
     3241 gaggcttcca ataaggccat gatgcgcgat gacatggtga atttctggaa gacgaattat
     3301 gtgccgaata acgccgcgct ggttgtgtcc ggaaatattc cggtcgaaga tttgaaagcg
     3361 ctggccgaaa ataaattcgg cgcgtggaaa gcgggagaaa ttccgcaatc gaacattggc
     3421 gatctggaat cgacaaaggc gaagattgta atcgtggacc gtcccggcgc gcagcaaacc
     3481 atggtgcgca tgctggaaac gggggtggac cgggcgacgc cggactatcc ggcgctggag
     3541 gtgatgaact ccgagcttgg gggacttttt tccagtcgca tcaacttaaa tctccgcgag
     3601 gaacatggct atacgtacgg cgcgggttct tccttcgtgt accggcgtcc gctgggatat
     3661 tttgcggtgg gaggtggaat tcgcacggac gtcacggcgc ctgctgtaac ggaaatcctg
     3721 aaagaaattc atcgcatgat ggatacgcaa ctgaagccgg atgagctgag cctcgcgaaa
     3781 gattctcaat cgcgatcatt gcccggaatg tttgaaaccg acaacgggga agcaggcgct
     3841 ctctccgaaa tttttgttta caatctggcg cgcgattatt tttcgacttt gccggagcgg
     3901 ttgaatgccg tgacggcgga ggatgcggag atggtggcga agaaatatct gcatcccgac
     3961 caactaattg tcgtgtgcgt gggagaccgg gcgaaaattg agccggaatt gctgaagctc
     4021 gatctgggcg cagtggaaat tcgcgatgcg gatgggaatg tggtgaagta attttcggaa
     4081 agcatcgggt taaaccggcc gcggagcgcg ctgccgcacg aaatctacga tgtcttgtgt
     4141 ggatgtgccg ggcagaaaaa ttgcggcgac tcctgctttt ttcagcgcgg gaatgtcggc
     4201 ttccggaatc gtcccgccaa gaagaacagt aacgtcgttc atgccttttt gtttcagcaa
     4261 gtccatcaat tgcggaacga gcgtgttgtg cgcaccggaa aggatggaca ggccgattac
     4321 gtccacatct tcctgaaccg cggcggaagc gatcatctcc ggcgtttggc gtagcccggt
     4381 gtaaatcact tccataccag catcgcgcaa agcgcgggca ataatttttg cgccacggtc
     4441 gtgcccgtca agaccgggtt tagcaattaa aacacgaatt attttctcag gcatcaggct
     4501 aaaccgtctc cggttttctc aagtttttct ctgtgctctc tgtgttaaag cttttgacct
     4561 gctacacaat aaaccgccgc actccatcct tcaataccgc aacgttaaag ttgatgagca
     4621 gaccggtctt gtgtccggac agccgaagat aagtgagaat aattgcctcg tgaatcggcg
     4681 cgaggttctc tacggatttt agttccacga tgatccgatc ttcgacgagg agatcaagcc
     4741 tgtaaccgca gtcaagctta ctaatcagag cattatatag gtgacagaca ggaagtaatg
     4801 tagaagctgt tgcataacct agtacaggga cttgaaaaga aggtagttga attttctggg
     4861 atgagagata aggggaatca gcagcgccca aggagggccg atggcccgat cgggtgcatt
     4921 gctgaccgaa gcgcagtggc
//
LOCUS       DASZMB010000230        10624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1035022, whole genome shotgun sequence.
ACCESSION   DASZMB010000230 DASZMB010000000
VERSION     DASZMB010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 10624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10624
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035022"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            386..1285
                     /locus_tag="VG272_10730"
     CDS             386..1285
                     /locus_tag="VG272_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWF14201.1"
                     /translation="MNRLVRKFIVPGLACAGLMAGLNCTPAKAADDAVVTADKAVVAA
                     LAKGDKAAANKWLDPDFSWIDSEGIMWAKDDAFRGGLKPLVLPGDSVKIIEHKYGKSV
                     VWIQWNQDNKYSARFWVKRPAGWKLLHTTEIAAMPKRDFQTVEPTYDIPCNNPCKAVP
                     YKPLSAGEKASLDEWQTQESSQENWEKHVADNLDQRVVSTYGGASPSKADRIVGMNKR
                     KAANPNAPKQGAAPALWIRTWDFGDAVVMVSVQPTYGDKAYWSSRVLAPNDTGMWQMM
                     ESYHNYILASPVMTATPNQPDLK"
     gene            complement(1378..1962)
                     /locus_tag="VG272_10735"
     CDS             complement(1378..1962)
                     /locus_tag="VG272_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HWF14202.1"
                     /translation="MKLRQIVTACAILGFSAVVSAPAKAQAPPAAAPTVPGLTLSSPD
                     FEDGSIIPGKFTQSVTDFVSPKLVWTNVPPNTMSFVLLAHDPDAALDKKTTDVLHWLA
                     FNIPGTATSLPQAVPPTAMMSDGTVQAKNRRGAVGYLGPGAPAVGPYHHYTFELFALD
                     TKLTLGADATRQDVLTAIDGHILGKGVLVGRFHK"
     gene            complement(2177..2827)
                     /locus_tag="VG272_10740"
     CDS             complement(2177..2827)
                     /locus_tag="VG272_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14203.1"
                     /translation="MKRSTVLAMCASAGIGLTLMMAGAAVHSSEKEQRFPVMKMEQLN
                     DQQKPFADEVLKVSSIGITGPYNMMLRSPVMGTRMFSMLDYLRFNTSVPRKLNEFAIL
                     IQGRLWTSQVEWSAHYPLALKAGLPQSVADDLKVGKRPASMAPDEEAVYDFCMELSTK
                     HFVSDATFKKIRAVFSDQQIVDLIGVTGTYITAAMLSNTNEDPTPGGKTPPLAPLP"
     gene            complement(3074..4777)
                     /locus_tag="VG272_10745"
     CDS             complement(3074..4777)
                     /locus_tag="VG272_10745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HWF14204.1"
                     /translation="MARILVIQLGRLGDVIQTTPLLRELVHDNHSGSIDVLVFDSLRE
                     IVEGIGLRKIHSFSERALPLTLRQLDKTIEENRKQLGAVPREAHEALQNLALPAYDHI
                     VNCSYSPLGAFVAQNARHKSIAGPVITFAGEILFHHPAQIYLCARASFRNQNWFNLVD
                     LWRASGRPYQNKVPAFIARPHVPIASEVPFDIPSGRIVALNPGSSDAARRWPPGEFAS
                     LAEGLVSHGLVPALVGAPSDRETCAEVQSRSTVPIANFCGKTSVPQLAFFLTHADLLV
                     TNDTGAIHIAAAAGCRSLSLFGSTAYFAETAPWATGHVILQGPLGSDLIVLHPKLVLA
                     SALYCLELIDEDILRTETARQDASAWETFFLDPQADPLGGISYRPLHGDHFSSDQIFA
                     RELRHLFAHVFSNAGNFPGVGDCSHLAGEFANRRIRTPVNERDLGVACAVAGLESMAE
                     AAARCGALCARQTRESAAEITSLSASLVGAMEELTTHAEIHTRMKPVIHFLDWTCRMM
                     DPMDPAPTFHAHQQAYKLAARMLSECEFRAQAKLQGADRFSRLSNQKVWDVQSPVDVP
                     I"
     gene            complement(4988..6772)
                     /locus_tag="VG272_10750"
     CDS             complement(4988..6772)
                     /locus_tag="VG272_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HWF14205.1"
                     /translation="MPSRIEDYAMIGDCETAALVAKDGSIDWLCWPRFDSSACFAALL
                     GTPENGRWILAPATPLKSTRRRYVPETLILETEFETESGTVAIVDFMPLRGRQSNLVR
                     IVRGVRGEVAMSMELILRFDYGESVPWVTRITETSIRAISGPNMVVLRTSVPVHGEDL
                     RTISEFTVAQGQAIPFVLSFTPSHLPVSSEIDAMKALDKTEKFWKKWSSTNTYRGPWA
                     EEVNRSLITLKALTYWPTGGIVAAPTTSLPEKIGGQRNWDYRICWLRDASFTLWVLMK
                     AGYFREATAWQNWLLRAVAGSPDQVQIMYGLAGERDLRELELNKLPGYEGSKPVRIGN
                     AAAEQLQLDIYGEVASLLHYARGEKLLTNGANIDLEWELLTHLEKIWRTPDEGIWEVR
                     GPRRQFTHSKVMCWFAFDSAIKSCERFGLKGPLDRWRLVREEIHQDVCRNAFDAELGS
                     FVQSYASKEVDASLLMIPKVGFLPVTDERVQGTIRAIEKYLMRQGFILRYNTKETIDG
                     LPPGEGVFLPCSFWLADVYFLSGRYRDGEQLFEKLMQLRNDVGLFSEEYSPGERRLAG
                     NFPQALSHVAFLNTIFISKRARDAQGKK"
     gene            complement(6910..7806)
                     /locus_tag="VG272_10755"
     CDS             complement(6910..7806)
                     /locus_tag="VG272_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14206.1"
                     /translation="MRIITYFRFGIGGKAWLVLVMFGIGLGGLSIAGLAAPASGGQNQ
                     GTDRVVREILENEIRAQKEDLSLWQYQENKIENGKTERFEVVETREGDLERLVSVNGA
                     PLAPDPEKKEEARIHRLVSDRQAIQTEHRKQQEDVVQELKLLHMLTTAFQYQDAGQDG
                     KLRKVNFRPNPTFHPPSREGEVFHHMAGSLWLDVEQKRLARMSGTLTSEVTFGWGILG
                     HLAKGGTFTVEQAEVGKGHWELTRLVVNMNGKALFFKTIDVKQNVQNSNFKPVPREVT
                     LQRAAELLKSDSTASARTMTGN"
     gene            8053..8238
                     /locus_tag="VG272_10760"
     CDS             8053..8238
                     /locus_tag="VG272_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14207.1"
                     /translation="MKYKVHLNIPTAWLGKVKVEADNPTDAGVRALEMAAKGDISFER
                     PEVDYADAEIADVEESE"
     gene            complement(8311..10302)
                     /locus_tag="VG272_10765"
     CDS             complement(8311..10302)
                     /locus_tag="VG272_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14208.1"
                     /translation="MHLLDSIIAFAAVMLAASLVVTAGTQLIISVLGLRGANLRRSLS
                     DLFENACDDRDAKRYGKVIARRALHHPLLTGSAFSRFGVRAGDLPFLPADAAGKLRWA
                     GSGIPFQPWLLGAASGFFLCPAAIAIINRLTPLDISAVSNLINGVVPLLNFVEHPWRT
                     GAIVGAIFGGLLSRWRLATSVRPDELVGVLEKLSSPPGGSLPDPAQRAMLVIAGEAQS
                     APRLKMDAAALEFDKFIRELPEHNEDAMLPASEKSQKPAGPQLESRLEGVNSWFDHVM
                     TRASQRFTLQARVITVAVSFVLVFGAHFDVIRIFHSLASDAEVRAQIAESTDALLKQA
                     AQLSHSREDGSSKEIVPDVYRKAMVDVLQSTPVSVESVKPKSHHSSHHSSSSAAAASQ
                     AAANGASAESPDGVQAGAQPPAEDAAASAAAQLASDAPVKESKHRSSKSSSKAKTPAP
                     VEAVKAPAAPMPGEDIATMQAKAAAGKALITTAGFASRQDAVAWLRATLNSNPATDNL
                     VANYEHAVNAQLVSDTDRLLDHSASMRYDFGRSELALVPEKWIGWKPAGNEWPGLLVA
                     VALLSLCAPICFNLLKTVASLRPIAFTGASYYPERRVRKEDRRQTPQRELSKIQLKAQ
                     PKAQEKPLEKATLKIPEKMSEKQEDDRKPVTAGHIDDLL"
BASE COUNT         2396 a         2795 c         3030 g         2403 t
ORIGIN      
        1 gcgcgaagat tatgtggaag aggcgtggag gattgtcgac cccgtgctga aagcggggac
       61 accggtttat ccgtatgcgc aaaatacgtg ggggcccgca gaagtggagc atgtaacgcc
      121 gccgggcgga tgggatgcgc ccgtggctca tgcggagggc tctttggctg aggattaaag
      181 gcgactcacg gtcggccttt gttgcggatt cgtttgtttg ttccgccacg attgctacga
      241 aggtgatttc gcgcgcggtg aaatttcacc gtgcgcggct ttgctgcctt ggacaagaat
      301 atcgtgcgga tgtgcgggat tgtactagaa tgggcggacg aatggtctgg attactcaac
      361 gagattggtg aaccaggggg aatttatgaa tcgcttagtc aggaaattta ttgtgcccgg
      421 gcttgcttgc gccgggctca tggcgggctt gaattgtacg cccgcgaaag cggcggatga
      481 cgctgtggta acagcggaca aggctgtagt ggcggcgctt gcaaagggag acaaggccgc
      541 cgcgaataaa tggctcgacc cggatttcag ctggatcgat tccgaaggaa tcatgtgggc
      601 caaggacgat gcatttcgcg gcggattaaa gccgctcgtg ctgccaggcg acagcgtaaa
      661 gatcattgaa cacaaatacg gcaaaagcgt cgtgtggatt cagtggaatc aggacaataa
      721 atattccgcg cgattctggg tgaagcgtcc agctggctgg aagctgctgc acacgacgga
      781 aattgcggcg atgcccaagc gcgatttcca aaccgtggaa ccgacgtatg acattccctg
      841 caacaaccca tgcaaggctg tgccgtacaa gccgctctcc gctggagaaa aagcttccct
      901 cgacgaatgg caaacgcagg aatcaagcca ggaaaactgg gaaaaacacg tggcagacaa
      961 tctggatcag cgcgtggtca gcacgtacgg tggagcgtcg ccttcgaaag cggatcgcat
     1021 cgttggcatg aataagcgta aggcggcaaa tccgaacgcg ccgaagcagg gcgccgcgcc
     1081 ggcgctgtgg attcgcacct gggatttcgg cgacgcggta gtgatggttt ccgtgcagcc
     1141 tacgtatggc gacaaagcgt actggtccag ccgcgtgctc gcgccaaacg acaccggaat
     1201 gtggcagatg atggagagct atcacaatta tattctggct tcgcctgtga tgaccgcgac
     1261 tccgaatcag ccggacttga agtagcagga cgcacggcaa aaaaataaaa acggcccggg
     1321 aaagttttcc tccctgggcc gtttttattt tcactatgcg gttttttggc aatgaagtta
     1381 tttgtgaaag cggcccacga gcacgccctt gcccagaatg tgtccgtcaa tcgccgtgag
     1441 gacatcctgg cgagtcgcat ccgcgcctaa cgtgagtttc gtgtccagcg caaagagttc
     1501 aaatgtgtag tgatggtagg ggccgactgc gggagcgccg ggaccaagat agcctacagc
     1561 gccgcgccga tttttcgcct ggacggttcc atcgctcatc atggctgtgg gtggaacggc
     1621 ttgaggaagg ctggtggcgg ttccgggaat attgaacgcg agccagtgca gcacgtccgt
     1681 tgtcttttta tcgagggctg cgtcgggatc gtgggcgagc aggacgaagc tcatcgtgtt
     1741 ggggggcaca ttcgtccaga ccagcttggg cgaaacgaaa tctgtgacgg attgcgtaaa
     1801 ttttcctggg atgatggagc cgtcttcgaa gtccggcgag ctcagagtca ggccggggac
     1861 agtaggtgcg gctgccggcg gcgcctgtgc tttcgcgggc gccgagacga ctgcggaaaa
     1921 acctaaaatc gcgcatgcgg tgacaatttg cctcaatttc atttgcattc ctctctttgt
     1981 taattcggcg ggtgtttttt gaattgctag ctcaggtaat gtacgccgca gcgtgggttc
     2041 ctgcaacggc agtgtgagtg cttccattta ttgaacataa aaagcagcac aggcttcatc
     2101 ccgtggggct agaccttgca aggactaaat tccacaggct gaagcctgcg ctacgaaaaa
     2161 cgaagtacat ttcgcgctac ggaagtggcg ctaacggcgg ggtttttccg ccgggagttg
     2221 gatcttcatt cgtattgctc aacatggctg cggtgatgta tgtaccggtc actccaatga
     2281 ggtcaacaat ttgctgatcg ctgaatacgg cccggatttt tttaaatgtg gcgtcactga
     2341 caaagtgctt ggtcgagagc tccatgcaga aatcgtaaac ggcttcttcg tccggggcca
     2401 tggacgcagg gcgcttcccc actttcaggt catcggcaac cgattgcgga agacccgcct
     2461 tgagcgcgag cgggtaatga gccgaccatt cgacctgcga ggtccatagg cggccctgaa
     2521 ttagaattgc aaattcattc agcttgcgcg gaacggaagt gttgaagcgc aggtaatcaa
     2581 gcatggaaaa catacgcgtg cccatcaccg gactgcgcag catcatgtta taaggtccgg
     2641 taatgccaat actggatact ttcaaaactt cgtcggcaaa cggtttctgc tgatcgttca
     2701 attgctccat tttcattact ggaaaacgct gttccttttc ggatgaatgc acagccgccc
     2761 ccgccatcat aagagttaaa ccaattcccg ccgacgcgca cattgccaac acggtgctac
     2821 gcttcatttc ttttcctcca ccatttttga atgaatgcac ggatttcttt agctgagagg
     2881 aacatatatc cagacgcggc ggaatgcaac gtcgcaacgc agggctgagt gaattgaaca
     2941 acgcgatgaa acggtattgt tctaccgagc ctcttcgaaa gtgtagattt cactgactca
     3001 cggactatgt gtggaatctt aatccccgtc tcttgagagg cgggctacag catcgggcgg
     3061 aatgcccagc ttctcagata ggaacgtcca ccgggctttg aacgtcccaa actttttgat
     3121 tagaaagccg cgagaatcga tcggcgcctt gcagtttcgc ttgcgcgcga aattcgcact
     3181 cgctgagcat ccgtgcggcg agcttgtatg cctgctggtg tgcgtggaat gtgggtgcgg
     3241 gatccatcgg atccatcatg cggcacgtcc aatccagaaa atgaatgacg ggcttcatgc
     3301 gggtgtggat ttcggcgtgc gttgttaatt cctccatcgc gcccacgaga cttgcggaca
     3361 gcgaggtaat ttccgccgca ctttctctgg tttgccgcgc gcacagcgca ccacatcgcg
     3421 cagcagcctc ggccatactt tccagcccgg ctactgcgca ggcaaccccg aggtctcgtt
     3481 catttactgg cgtacggatt cgccggttgg caaattcgcc cgctaggtga gagcaatcgc
     3541 cgacgccagg aaaattcccg gcattgctaa aaacgtgagc gaaaaggtgg cgcaactcac
     3601 gcgcaaaaat ctgatctgaa gaaaaatggt caccgtgtaa gggcctgtaa gaaattccgc
     3661 cgagcgggtc ggcttgggga tcaaggaaaa atgtttccca cgcggaggca tcctgccgcg
     3721 cagtttccgt tcgcaaaata tcttcgtcaa tcagttccaa acaatataga gctgacgcca
     3781 aaactaattt cgggtgcagc acaatcagat ccgatcccaa cgggccctgc aggatcacat
     3841 gtcccgtggc ccacggcgcc gtttcggcga aatatgcggt ggaaccaaac aatgaaagag
     3901 agcggcatcc ggcggccgca gcgatgtgaa tcgcacccgt gtcgttggtc acaagaaggt
     3961 cggcgtgtgt aagaaaaaaa gccaactgtg gaactgatgt tttgccgcaa aagttggcga
     4021 tgggaaccgt gctgcgggac tgcacctccg cgcacgtttc gcgatcggaa ggagcgccga
     4081 caagcgcggg cacgagtccg tgcgagacca aaccctcggc gagacttgcg aattcaccgg
     4141 gaggccagcg gcgagccgcg tcactcgaac ctggattcaa ggccacaatg cgcccggacg
     4201 gaatatcaaa agggacttcc gaagcgatag gcacatgggg gcgggcaatg aacgcgggca
     4261 ctttgttctg gtaaggtcgg ccgctcgcac gccacaaatc gacgaggtta aaccagtttt
     4321 gattgcggaa actcgcgcgc gcgcacaaat aaatttgcgc cggatgatga aacaatatct
     4381 cccccgcaaa ggtgattacc gggcccgcaa tgcttttgtg ccgggcattt tgcgcaacga
     4441 atgcgcctag tggcgagtag ctgcaattca cgatgtggtc gtaagcggga agcgcgagat
     4501 tttgcaaagc ttcatgcgcc tcgcggggta cagcgccgag ttgcttgcga ttttcttcga
     4561 tggtcttgtc gagctgccgt agggtgagcg gaagcgcgcg ctcagaaaat gaatgaattt
     4621 ttctcaggcc gatgccttcg acgatttccc tgagcgagtc gaagaccagg acgtcgatgg
     4681 agccggagtg attatcatga accaattcgc ggagcagagg cgtggtttgg atcacatcgc
     4741 caaggcgacc gagctgtatc acaaggatgc gcgccatacc gctccatttt taaaaccatg
     4801 ggcgcaggaa atcgtaatgc ccgcccaaca tgcatatcgg caacgggatt gaatacgtga
     4861 gcgccagaaa gatctacgaa gtgacttcgt tgtgaggcga tccggcctgg tgcgcagtag
     4921 agtggcaaat tacacccagg ctttccagtc actctaaatt tcactggaag tgcaacgagc
     4981 agttcattta cttctttccc tgcgcgtcgc gcgcacgctt ggaaatgaaa atggtattca
     5041 gaaacgcgac gtgggacaac gcctgaggaa aatttccggc taaccgtctt tcgccaggac
     5101 tgtattcctc ggagaacaaa cccacatcat tgcgcagctg catcaatttt tcaaaaagct
     5161 gttctccatc ccggtatctg ccggataaaa agtaaacatc cgctagccaa aaactgcatg
     5221 gaagaaaaac tccctcgcca ggcgggagcc cgtcgatcgt ttctttcgtg ttgtaacgga
     5281 gaataaaccc ttggcgcatc aaatattttt caatcgcccg aatcgtgcct tgcacccttt
     5341 cgtccgtcac tgggaggaaa ccgacttttg gaatcatcaa tagactggcg tcgacttctt
     5401 ttgaggcgta ggactgcacg aagcttccaa gttcggcgtc gaatgcgttg cggcagacat
     5461 cctggtgaat ttcctctcgc acgagccgcc agcggtctag cggccccttc agtccgaacc
     5521 gctcgcaact tttaattgcg ctatcgaatg cgaaccaaca catcactttt gagtgagtaa
     5581 attgtctgcg cgggccgcgg acttcccata tgccttcgtc gggcgtacgc cagatttttt
     5641 caagatgtgt aagcagctcc cattccagat cgatgttagc gccattggtc agaagttttt
     5701 cgccgcgagc gtagtgcaat aggctggcga cttccccgta aatgtcgagt tgaagctgct
     5761 ccgccgcagc gttccctatt cgcacgggtt tcgaaccttc atacccgggc aatttattca
     5821 gctccaattc cctgaggtcg cgttctccgg ctagtccgta catgatttga acttgatccg
     5881 ggctcccggc aacggctcgc aacagccagt tttgccacgc ggtcgcctcc cggaaatatc
     5941 cggccttcat caatacccac aacgtgaagc tcgcgtcgcg aagccagcaa atgcggtaat
     6001 cccaatttcg ctggccgcca attttttccg gaagtgacgt cgtgggcgcc gcgacgattc
     6061 cgccagtcgg ccagtaggtt aaggctttca gagtgatgag ggaacgattg acctcttccg
     6121 cccacgggcc gcgataggta tttgtgctgg accatttttt ccaaaatttt tcggtcttat
     6181 caagcgcttt catcgcgtcg atctcgcttg aaacaggcag gtgcgacgga gtaaaactca
     6241 gcacgaacgg aattgcctgt ccctgcgcga cggtaaattc agatatcgtt cgcaagtctt
     6301 ccccgtgcac gggcacggat gttcgcagca cgaccatatt cggcccggaa atcgctcgaa
     6361 tcgaagtttc ggtgattcgc gtgacccacg gaacggattc tccgtaatcg aaacgaagaa
     6421 ttaattccat actcatcgct acttcgccgc gaacgccgcg aacaatccgc acgagatttg
     6481 attgcctccc gcgcagcggc atgaaatcga cgatggcgac cgtgccgctc tccgtttcaa
     6541 attcggtttc cagaattaat gtctcgggga cgtagcggcg gcgcgtcgat ttaagcggag
     6601 ttgccggtgc aagaatccag cggccatttt caggtgtgcc cagaagcgcg gcgaagcagg
     6661 cgctcgaatc gaagcggggc cagcatagcc agtcgatcga tccgtctttc gccacgagtg
     6721 ccgcggtttc gcagtctcca atcatggcat aatcttctat gcgagaaggc atgcaggtcc
     6781 ccacttttgt gtgaatcaat tgttacgagg cgcgatgcgt cgaaaaacgc ctccgaatcg
     6841 gacggcatat agattcccgg ttcacaaaaa tcaatatctt gcagataaaa ctcagctgta
     6901 cgactggaat taatttcccg tcattgtcct ggcggacgca gtggagtcgc ttttcaaaag
     6961 ctctgccgcc cgttgcagtg tcacttccct tggaacaggt ttaaaattag aattctgcac
     7021 attctgcttc acgtcgatcg ttttgaaaaa gagcgccttg ccgttcatgt tgacgaccag
     7081 ccgtgtgagc tcccaatgtc cttttccaac ttccgcctgt tcgactgtga aagtgcctcc
     7141 cttcgcaagg tgacccagga tgccccagcc aaaagtcact tcgctagtga gcgtgccgga
     7201 catgcgcgca agccgctttt gctccacgtc cagccacagg gagcctgcca tgtggtgaaa
     7261 aacttcgccc tcacgagagg gaggatgaaa ggtggggttc ggtctgaaat ttacttttcg
     7321 taatttgccg tcctgtcccg cgtcctgata ttggaatgca gtggtcagca tgtgcaggag
     7381 tttcagctct tgcacgacat cttcttgttg tttccggtgt tcggtctgga tcgcttgccg
     7441 gtcagatact aaacggtgga ttcgcgcttc ctcctttttt tcgggatcgg gcgctaacgg
     7501 cgccccgtta acactaacca gccgttcgag gtcgccttcc cgggtttcga caacttcgaa
     7561 ccgttcggtt ttgccgtttt cgattttatt ttcttgatac tgccataggc tcagatcttc
     7621 cttttgggct cggatctcgt tttccaatat ttcgcgcacg acccgatccg ttccctgatt
     7681 ttggcctccg ctagcgggcg cggccaatcc cgcgatggat aacccgccca gtccgattcc
     7741 aaacatcaca agaacaagcc aagccttccc tccgatgccg aatcgaaaat aagtgatgat
     7801 gcgcattccc gttgtcaccc ccgataagac cggtactttc ccctgacgat gcgaatccca
     7861 ttttatttca ggaccggcgc gcgaatccca caactgcggt atctaaattg gacaattgag
     7921 gcttttcgtg taggactttc aatagttttg aagtttgatg ggttttgcag ggtacaaaat
     7981 tcccggtatt gctcgtcccg aaccgtttgt gtcttctaaa gaggtctcgg agtgaaggag
     8041 aaatgagggg ctatgaaata taaagtacat ctgaatattc cgaccgcctg gctgggaaaa
     8101 gtgaaggtcg aggccgataa tccaacggac gcgggggtga gggcattgga aatggccgcg
     8161 aagggtgaca tctcgtttga gcgtcctgag gtggactatg cggacgcgga aattgccgat
     8221 gtcgaagaat ccgaataaga gaacggattt ctcggtaatc agtacgtttt tgaaatttgg
     8281 aatctaagtg tccgcctcta aagagacggg ctacagcagg tcgtcgatat gacctgccgt
     8341 gacgggcttg cggtcgtctt cctgcttttc tgacattttt tctggaatct ttaatgtggc
     8401 tttttccagt ggtttttcct gggctttcgg ttgcgctttt agttggatct tcgaaagttc
     8461 tcgctgtgga gtttgacggc gatcttcttt tcgcactcgc ctctcaggat agtagcttgc
     8521 gccggtgaac gcgatcgggc gaaggcttgc gacggtcttt aataaattaa aacaaatggg
     8581 cgcgcagaga ctgaggagtg ccacggcaac cagcaagcca ggccactcgt ttccagcagg
     8641 tttccagcct atccattttt cgggaaccag cgccaattcc gaacgtccga aatcgtaacg
     8701 catggacgcg gagtggtcga gcaatctgtc tgtgtcactc acgagctgtg cgttgacggc
     8761 gtgttcgtaa ttcgccacca gattatcggt ggcggggttc gagtttaaag tggcgcgaag
     8821 ccaggcgacc gcatcttgcc ggctggcaaa tcccgcagtg gttatgagag ctttgccggc
     8881 tgctgccttt gcttgcatcg tagcgatatc ttctccaggc atgggcgcgg ctggggcctt
     8941 tactgcttcg actggagcgg gcgtctttgc tttcgaagac gacttcgaag accggtgctt
     9001 cgattccttt acgggcgcat cggatgcaag ttgagcagcg gctgacgctg cggcatcctc
     9061 ggccggcggc tgagcgccag cttgaacccc atccggcgac tccgctgaag cgccgttggc
     9121 cgcggcctga gaagcagccg cagctgacga agaagagtga tgcgaggagt ggtgcgactt
     9181 cggctttacc gactctacgc tgacgggagt agattgcaaa acatccacca tcgctttgcg
     9241 atacacgtcg ggcacaattt ctttgctgga accatcttca cgcgaatgag aaagttgcgc
     9301 cgcctgtttt aaaagagcat ccgtgctttc agcgatttga gcgcgcactt ctgcatcgga
     9361 agcgagtgaa tgaaatatgc ggatgacgtc aaagtgcgca ccaaaaacga gcacgaaaga
     9421 cacggcaacg gtaatcacac gggcttgcaa ggtgaaccgc tgggacgccc gggtcatcac
     9481 gtggtcgaac caggaattga ctccctccag ccggctttcc agctgcggtc cggcaggttt
     9541 ttggcttttc tccgaagccg gaagcatggc gtcttcgttg tgctccggca attcacgtat
     9601 aaatttgtcg aattccaggg ctgcggcgtc catcttgagg cggggcgcgc tctgagcttc
     9661 accggcaatt acgagcattg cacgctgcgc cgggtccggc agcgaaccgc ccggaggcga
     9721 agaaagcttt tccagcacac ccaccagttc gtcggggcgg acggacgtgg ccaatcgcca
     9781 gcggctgagg aggcctccga aaatagcgcc gactatcgct cccgtgcgcc agggatgttc
     9841 cacgaaattc aggagcggga cgacgccgtt aataaggttc gagaccgcgg aaatatccag
     9901 cggcgtgaga cggttgatga tggctatcgc tgcggggcag aggaaaaagc cactggccgc
     9961 gcccagcagc cagggctgga atggaattcc gctgccggcc cagcggagtt tccctgccgc
    10021 atctgcgggc agaaacggaa gatcgcccgc gcggacgcca aagcgagaaa acgcggatcc
    10081 ggtcagcaaa gggtgatgca aggcccgtcg cgcgatgact ttcccgtagc gcttggcgtc
    10141 ccggtcgtca caggcgtttt cgaacaggtc agaaaggctc ctgcgaaggt tggcgcccct
    10201 caggcccagc acgcttataa ttagctgggt gccagcggta accaccaaac tagctgccaa
    10261 cataaccgcc gcaaacgcga tgatcgaatc gagaagatgc atagttttcc gctctccatc
    10321 tgctccctaa agtttaggtt atgagcaatg agccgggcgg aagaaaaagg tactggagta
    10381 gcctcttgtt gacctgtacg gaaggacagg tggcatgata ggtgccgagg agcaaggata
    10441 aacgagggtt agggcgtcac ctagcagcgg tcgagaggct cgcaggcact aaatcacatc
    10501 aagttggcaa ttttgatcca tatagctatg ctgaaactgt ggatgtctgc gcccgaaggc
    10561 ggcggggcgc tggctgacct ttattgttca gtaagtgggg cgggaattga aaacgaggaa
    10621 gcgt
//
LOCUS       DASZMB010000231        44014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4623395, whole genome shotgun sequence.
ACCESSION   DASZMB010000231 DASZMB010000000
VERSION     DASZMB010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 44014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44014
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4623395"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VG272_10770"
     CDS             <1..404
                     /locus_tag="VG272_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14209.1"
                     /translation="KTGKITYYKTPTPNSAPRRGSVDSAGHIWFGEYRGDRIGMFDPE
                     TEKFQEWTPPTPWALPYDVVADKNGDVWAGSMVNDRVTRLNPKTGDMTDYLLPRETNI
                     RRVFVDNSTTPVTFWVGNDHAASIIKLEPLD"
     gene            593..823
                     /locus_tag="VG272_10775"
     CDS             593..823
                     /locus_tag="VG272_10775"
                     /inference="COORDINATES: protein motif:HMM:NF021558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HWF14210.1"
                     /translation="MTIEQQVVEKLRDLPPEKQKEVLDFVDFLREKNGGPKKPRRSLL
                     GLWADLNIHITEEDIADARREMWGNFPRDIEL"
     gene            820..1218
                     /locus_tag="VG272_10780"
     CDS             820..1218
                     /locus_tag="VG272_10780"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HWF14211.1"
                     /translation="MSAVLDTHTVLWYLENSKELSGVARITIEDAVRDARDVHVSAIS
                     LVETVYLVERRRLPLNALERLRSALNDPNSGLFIAPVNGSVADALPKIPRDVVPDMPD
                     RIIAATALHLGLPLITRDRRLQSAGIQTIW"
     gene            complement(1222..1884)
                     /locus_tag="VG272_10785"
     CDS             complement(1222..1884)
                     /locus_tag="VG272_10785"
                     /inference="COORDINATES: protein motif:HMM:NF037417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HWF14212.1"
                     /translation="MIYELEPFHRDIPDEDLLADLRRVAKDLGKNRVTYREYNERGRF
                     TAGTLAERFGSWFAAIDKANLERTMNRNISDEELFVNLVEVWTALGRQPRSRDLRPGP
                     SRFSWATYAYRFSTWRKALAEFVRWANDGKFVPDSEGSAKKSGKRTPRNINWRLRALV
                     LMRDRARCRLCGAGPSEGVTLHVDHVKPWSKDGETVIENLQILCNVCNIGKGDIELKT
                     DN"
     gene            complement(1984..2060)
                     /locus_tag="VG272_10790"
     tRNA            complement(1984..2060)
                     /locus_tag="VG272_10790"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2024..2026),aa:Ile,seq:gat)
     gene            2300..3382
                     /locus_tag="VG272_10795"
     CDS             2300..3382
                     /locus_tag="VG272_10795"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWF14213.1"
                     /translation="MKLIKPKFVVILVLTFLTLFSSAGNVRAQDKRTGRSSGPIVTGK
                     VYAFEKIADGVYYTTSDSPMATGGNHTIIVGDQNVFLVDAGTTPAAARALLADMKLIT
                     DKPVRWVVNTHFHFDHTDGNSIFGPDVEIIGHEYVRHMIADEDVLHIEPFKTAMMNMP
                     MQVKALKKQIADEKDSAKRATLEGQLAAKQTDWEEFKMLKPTPPNVTYSSKMTVFQGS
                     REIQLLFLGRGHTQGDTVVYLPKERIVCSGDMMETQPAYMGNAFFDDWIATLDKLKEM
                     NFETMLPGHGVPFHDKALITAYQSYLKDLMTQVAALRKQGVSADDAAKRVDMTSHKAD
                     FPQIQGPGAEVRGVRRMYEWMDGQSR"
     gene            complement(3494..3958)
                     /locus_tag="VG272_10800"
     CDS             complement(3494..3958)
                     /locus_tag="VG272_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14214.1"
                     /translation="MKRRVAVFAAFLVVLTAGSVVLAQKNPFVGTWKLDVASSKYNPG
                     PAPQSQTRTWEASGKVTVDSVGATGKASSYGYTIKGDGKEYPTVGAIPNTADTITSKK
                     VDASSYEAKFFKAGKQVELTTFAVSNGGKTLTIHAKGTSPAGDFDNTMVWSK"
     gene            4342..4953
                     /locus_tag="VG272_10805"
     CDS             4342..4953
                     /locus_tag="VG272_10805"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWF14215.1"
                     /translation="MITRKIGEGGRWLLEKIVDAVAATGINPNLLTFFGLLVNCWAAY
                     LFAIGRFRGGAMVIFFAAFLDMLDGQVARREKRVTAFGAFFDSTLDRYSDMALYMGLL
                     VYYAVSQRTSYVVLSAVATAGSVMVSYSRARAESLIPLCKVGFMERPERMVLLILGGL
                     FNRMAPALWVIAIVATLTVIHRIVHTWQETKAGRTLPGIRLSL"
     gene            complement(4992..5813)
                     /locus_tag="VG272_10810"
     CDS             complement(4992..5813)
                     /locus_tag="VG272_10810"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWF14216.1"
                     /translation="MNGESEQPIPVPAATELAGEHGLRRAWAIARSTYHWLLSAAFFF
                     PVASFLVLLGIFIDPRKNDGAQRLLCRVVMKLAGVNVVVQRSPGFDPARTCFFLVNHV
                     NLFDPFLLYATIPQFLRGLELESHFRIPVYGWMMKRFGNIPVPDVNRPADLKRMWRLT
                     RAALDSGVSLAVFPEGQRTRDGHVGPFKDGVFRMALQFGTPIVPVSFVGAFEFNKKTS
                     WLIRPGTVTVHLHDVIETKDLRKNDFPALRDRVHAIVAEPIEQARARTEKLKDLV"
     gene            complement(5810..6715)
                     /locus_tag="VG272_10815"
     CDS             complement(5810..6715)
                     /locus_tag="VG272_10815"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HWF14217.1"
                     /translation="MNEAYFTIVNPAAGGGRCGKLAPKALHRLRAAGLQLEVRETRAP
                     GEGTVYANGAYADGFRRFIAVGGDGTSFEIVNGIFPEAAIEGRASLGFLPLGTGNSFL
                     RDFVQNGTDLAEFGINAILERRSRPCDVIRLNHSGGSIYYINTLNMGFAADVATLTNR
                     HLKFLGEPGYLVGVLVSLARLHRRPFPLRADGDWDDRRCLFVAFSNSKYTGGKMMIAP
                     QASIDSGAIEYVHWGPIGRLSLVRNLPTLFTGTHIRHPMASRRTATRIQFDLKNPVDV
                     VVDGEVLTLEAESLEVLPGALNVMV"
     gene            complement(6712..8433)
                     /locus_tag="VG272_10820"
     CDS             complement(6712..8433)
                     /locus_tag="VG272_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWF14218.1"
                     /translation="MAILMLDLNKYDSVGAALRDALGRWPDEVCLIEADREKEKSRLT
                     YKQFGEAAEPIAAALEESGFAAGNRAAIIMTNQPKWLISAYAVFFAGGILVPLDYKLS
                     AGEQLQLLAHSKAQILFVEYPLWRAITQSPAFAEHKLKTVLVSEAPQNADLAGAQRWE
                     EFRGTCAPEFKPRTRKDTACVVYSSGTGGHPRGCVLTHDNYLEQCAAVTPVFPFWPGA
                     RYLSIIPTNHAIDFMGGFIMPFTGGGTVVHLRTLRPEFIREAFTKYKITYMAVVPLIL
                     KNLQRGLREKFAALPSGKRKMLDALISVNRALTRGKPRPGLSRTLLKEIHKAFGGGLQ
                     ALLVGGAFTEPATIEFFHDIGIPIYNGYGCTEACTAITLNDLRPFRPDTVGKPVRGME
                     IRIAQPDADGIGEVAVRSKTVMSEYLDDPEMTAETIVNGWLMTGDLGRLDATGHLQLF
                     GRKKNMIVTAEGKNIYPEDIENNFEGIAAKEFCVFAANYIWPQHTMTGEQLVLVIHPE
                     PGQTVNDFLVKELSRRNSQLLNYKRVAGYIVWDQDFTRNAAMKIRRPILAGQIGDQLD
                     RSAMVAL"
     gene            complement(8477..8848)
                     /locus_tag="VG272_10825"
     CDS             complement(8477..8848)
                     /locus_tag="VG272_10825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HWF14219.1"
                     /translation="MPDINKLTEKIIGCAIETHRELGPGLLESTYEAALAIELEDAGI
                     LFERQVIFPVIYKGRKIGEYRLDMPVENKVIVEIESVERFDRIFEAQILTYLRVTGTH
                     VGLIVNFNSRLLHEGIKRFIQ"
     gene            complement(8937..9179)
                     /locus_tag="VG272_10830"
     CDS             complement(8937..9179)
                     /locus_tag="VG272_10830"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWF14220.1"
                     /translation="MKTIEETTDEILNLAATHFKFPRTDLAATDDFYKKLGIDSLQAL
                     ELLTRLENHFGVELPDYEVQGVSDFHTLATRIQARL"
     gene            complement(9274..10077)
                     /locus_tag="VG272_10835"
     CDS             complement(9274..10077)
                     /locus_tag="VG272_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HWF14221.1"
                     /translation="MDGYKEKTLSWRLADGVIELALNRPPANEIGLAMLDDLERFVAA
                     LPALEAKASTLILHSELKAGFSAGADLRELYEEAQKIDTSAAEKAVRAFLERIHAVLN
                     RLDTSSLTTIAAVHGVCFGGGFELALVCDLIIADKMARFCFPELRLGLIPGFGGIPRL
                     KRDIGNGVVRDLLLTGRSINATKGLSVGLVSQVTAEGEALRVARLTAAQTTKFDRGTS
                     SAAKQFIKPLPAEELRREIDIFCDLFTRPAVMEGLRKFVENTGAHPYLP"
     gene            complement(10224..11870)
                     /locus_tag="VG272_10840"
     CDS             complement(10224..11870)
                     /locus_tag="VG272_10840"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWF14222.1"
                     /translation="MNFIENIFARLQESSSRVVLAEAHADGVRTATGSDLLAQIAAAR
                     AFLRASGLAKGDRCALVAPNGIRWAALDLAIISEGLIAVPMYARQAAGELAAMLRDAQ
                     PALICCGDSTLRDAISAQWPEAPRFVLFDDIFNSKSSAATIAPLPPATLSPQDTVAIL
                     YTSGTSGEAKGVMLTVGNLDHMLSCTKSRLDNLMGTRNVPDRVFHYLPFCFAGSWILL
                     LSCLSRNSVLTMSMDLTKLADEIRAAEPDYFLNVPALLERIRTGVADNIRKRGGAIAR
                     IFENANAAWLRLDAKAPHAWDFFWLGFAGLLIFPSIRKRMGPNLRALICGSAPLARET
                     QLFFMMLGIRVLQVYGLTETTAICTMDDPQHVEPGRVGPAIPGIEMKIADDGEIIVRG
                     PNIFPGYWRRPEQTATGLRDGWFHTGDQGEVDAVGNWRITGRIKNLIILNSGHNIAPE
                     PIEEELFRAISGAQHVMLVGNGRSFLAAIITGTPGAEIDSQLERINTALPHYRKIRKY
                     HAAQELFTIENGLLTANGKLRRDVIAAHFTAEIERMYEKD"
     gene            complement(11961..13397)
                     /locus_tag="VG272_10845"
     CDS             complement(11961..13397)
                     /locus_tag="VG272_10845"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HWF14223.1"
                     /translation="MMDFILEAWTSSYIELSSMLWKFGAGRSWQPGEKLKLLFAGYNG
                     TRNTGSDVRVEEMLRQVRRILGEENVALSVMTFDPNRSRGYFEGTAQVQLPMIFPPFL
                     FSEVRKHHGVITCEGSMFKSKFANALTTMAIGALGIASAENKLSIGYGGEAGAMDPML
                     EKMCAYYCRRSLIVTRNEESQAALSKLGIPTELGTDTAWTFEPRPADFGHKALSDAGW
                     DGRTPVLALCPINPFWWPVKPSLTKWFGRKLFGAHKESHYSSVYFHNAGPAVDAAYAN
                     YLASIAGAVKAFRAKKNVFPILVAMERLDARACHALTPQLGGVPVFTSDAHDMYELVS
                     ILRGSSMIVSSRYHAMVTSMPGLVPSAGITMDERIQNLLRDRGHENLLLTVDDPDLEA
                     KLFAVMENLSNDAENIRHGIGRCVVRNLKSMARMGMYLERAVQERYPGFPTRSGVRGW
                     EEYLPPLSPNLGKLLDTYENTAVSAAAS"
     gene            complement(13394..14572)
                     /locus_tag="VG272_10850"
     CDS             complement(13394..14572)
                     /locus_tag="VG272_10850"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWF14224.1"
                     /translation="MAIFLTGSTGYIGAHVTSILLERHADRLNLLVRAKTEQEASERL
                     WHAMQLHMDFPQFRDALATRISIFRGDLTDPRFGLGAADYTHLIGTTESVIHCAASLN
                     RKSEKSCLNVNLRGTLEVAQLARRSQDAHGLRRFSQVSTVAVAGHRSHEVVAEDLSID
                     WNRSDYDPYARTKKFCEHMVRELLPDVPRTVFRPSIVLGDSRRAETTQFDMVRAFVFL
                     AGLPLLPFRATDRIDIVPVDYVAESVAMLHQKPNPEHEIYHLSSGTGAETFGQLTHAL
                     AKSAGKMEPLFWPGLEGNFSAVVNMLSNSRGTIGRSASLLKVFMPYLVWDTVFDNTRV
                     VAEMGRTPEPFSKYCYPLLQFSRDHHFSYPYKAWTAGSDLQTEKVTTASAVSARGSHP
                     "
     gene            complement(14576..16999)
                     /locus_tag="VG272_10855"
     CDS             complement(14576..16999)
                     /locus_tag="VG272_10855"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWF14225.1"
                     /translation="MSNQSAQNIIDGHHANGSFPASPPQPDPRALTFWRVEGSLVALG
                     AVRPVAYFTWNSHTFAGRWARRGGALALALIRPFLYASHRVFATRVLHTLLAGVTRDR
                     LDSLGEEYFEYVLKKQLKKEGVERLRACLTEKGSENVVLVSQGLDHVMRPLAQYLGVS
                     RLVSNRLEFRKGVATGRLLDPVIRPRSPIARAFERSANGSVPYERLLKNLGMADHPEE
                     LRKAILPADRPKEVDHRALVVFNGRKQSEPLSVRETLAGKKILLIGVTGFIGKVWLAQ
                     ILSEVPKVGKIYLLIRRQRTTTSQRRFEKIFEESPVFDPLQEQLGNRFAEFVSERVEV
                     IEGDVSQPGLGIAPETRAQLARNLDLVVNSSGLTDFNPDLREALASNVAPVLHLIEFL
                     RESDHAALMHLSTCYVVGGKDGRVVEEVQPNYTPLGVEDFDAEREWHSLQEVVARAEA
                     RTESPEITAALRRQALGKHRGSVEPAGPELDNLIRKNRMRWLRNRLTRAGTRRARRLG
                     WPNTYTLSKSLAESMIVKHGAGLPIAIVRPSIVETSTDQPFRGWNEGINTSAPLSYLL
                     GTYFRQLPTNERKCLDVIPVDMVCRGMTLIAAAIIARRHEPIYQLATSASNPCDMGRS
                     IELTGLAHRKHYRSQQSLENWMRMQFDTIPVSKTRYEKFSVPAQKAVVRGINRFASAL
                     QMKKPPLAKAERDLVRVEKLIELYEPFILHNEQLFESQNVQLLSAALPADERKAFGYD
                     PSSVDWWDYWINIHVPALRRWVYPLIEGRPMEVRPHRSFTLHHNEDGVQSPVNVPHAS
                     AESRTRIQN"
     gene            17579..19318
                     /locus_tag="VG272_10860"
     CDS             17579..19318
                     /locus_tag="VG272_10860"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWF14226.1"
                     /translation="MASKWKAGILTAALTLSTMSTAYAGPKNGQWDGNGHRRIQHVLL
                     ISIDGMHVVDFANCVTSDTCPHLAELGESGVTYTRTSTSRPSDSFPGLMALVTGGTPR
                     TVGAFYDVAYDRVLAPPATTTGNGLAAGSCIQGVINGTQTEYEEGDEIDQTKVNGGGP
                     YASSIDGGILSIDPTKLVRDPFNGCKPVYPWNFVRTNTIYGVIHKAGGYTAWSDKHAV
                     YAVVSGPTGTSLPSNVDDYYSPEVNSNQVPLAGITTAGTNFDCSQIAASGNDWTTDFQ
                     AIQCYDQLKVNGIVNQIKGKKHLGVGDAPVPAIFGMNFQAVSVGQKLIENGVKGGYTD
                     AAGDPTPSMASEIKFVDAGIGQMVEELQDRHLLSSTAIIITAKHGQSPIDTNRFFPIP
                     GNSGLNGTPPSGIIGTLLPLSATTGLGLAEDDISQLWLTNSNDTLTAVGMLESNAAKA
                     GIGQILYGTSLNTIFNPPGVPTTPGPCCFLREGGDPRTPDIIVLPNVGVVYTGSTKKQ
                     SEHGGFAWDDTNVMLLVSNPGYKAQTLHSFVETTQVAPTILKLLGLDPDELDAVRKEG
                     TPVLPGIDFGDGE"
     gene            complement(19573..20667)
                     /gene="leuB"
                     /locus_tag="VG272_10865"
     CDS             complement(19573..20667)
                     /gene="leuB"
                     /locus_tag="VG272_10865"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HWF14227.1"
                     /translation="MTKQTKKIVLLPGDGIGPEIMRVAVRILKVCATEFGHEFDFAEF
                     PFGGCAIDATGEPLPAETLAACKTADAILLGAVGGPRWDAVPLVKRPETGLLALRKAL
                     GLYINLRPIDLRLPLYGLSPLRADRVKDVHIDFVRELAGDIYFGKHDVQPDPSGERAT
                     DEAVYTTAEVERVAKYAFARAESRKGKLASVDKANVLTTSQLWRRTVTRMSANHPNVK
                     LEHFYVDNAAMQLVLAPAQFDVILTSNMFGDILTDLGAALAGSIGLIPSMSSGPGPSV
                     YEPIHGSAPSLAGKDFANPVGMILSGAMMLRESFNMQREAQYIESAVDRLFTKGIRTP
                     DTVEPNTTKVGCVEFGVRLQTEMTGAAHSL"
     gene            20851..21225
                     /locus_tag="VG272_10870"
     CDS             20851..21225
                     /locus_tag="VG272_10870"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWF14228.1"
                     /translation="MVKYNRELDATFQALADPTRRAIISALALGQASVTQLARPHRMS
                     LPAIMKHLVVLQKAGLVTQRKVGRTRHCRLAAASLKKAEAWISQYRAFWETQFDSLDR
                     YLSEQPKEDSQWPKSSFPRKPR"
     gene            21189..21641
                     /locus_tag="VG272_10875"
     CDS             21189..21641
                     /locus_tag="VG272_10875"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWF14229.1"
                     /translation="MAKEQLSPETSLTLRRTFLAPRERVFRAWTDPVELGRWFAPSDE
                     YHAIVPEHELRVGGKYCLEMHHNGGNIHRVSGIYKEINPPEKIAFTWLWDHEPKSDES
                     LVTVEFRDLGPSTEILLTHARLPNAEQTEKHGHGWNGCLNMLGNYLNM"
     gene            21713..22210
                     /locus_tag="VG272_10880"
     CDS             21713..22210
                     /locus_tag="VG272_10880"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWF14230.1"
                     /translation="MAIVDSILMEIEQEAKVTQKVLERVPTDKLTWKPHPRSLSLGQL
                     AMHIAVGQGRLAEIISKDTHEIGDISQPQPGPDDDILKTFNQGTANALGTLKKLNDSQ
                     LMALWTLTKGGKVVLSAPRIGVIRSILMNHFYHHRGQLSVYLRMLEVPIPSIYGPSAD
                     ENPFA"
     gene            complement(22412..23602)
                     /locus_tag="VG272_10885"
     CDS             complement(22412..23602)
                     /locus_tag="VG272_10885"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HWF14231.1"
                     /translation="MEIVRIFDTTLRDGEQSPGFSMSVPEKVRMAHQLESLGVDIIES
                     GFPIASAGDLEGTRAVCREIRGCTVAALARARRDDIDAALDGLSGAAHPRLHTFLATS
                     DLHLQHKLRLTREECLEAISNNVRYAREHCAEVEFSAEDASRTEIGFLCEAVGRAVHG
                     GATIINLPDTVGYAVPEEYAAMFRRVIDYLDGADGITMSAHCHDDLGLGVANTLAAVQ
                     AGVRQLELTINGIGERAGNAALEEVVVLLNVRQATLGVTTNVHLDQLFATSRLLTEIT
                     GAVVAPNKAVVGANAFAHEAGIHQDGILKNPLTYEIIPPEAVGVPKRSMVLGKHSGRN
                     ALRATLLDLGFQPTREDLDECYKRVVTLADASKNVSNRDVLAIAHQVMRKRAGETAAA
                     AESD"
     gene            complement(23755..24237)
                     /locus_tag="VG272_10890"
     CDS             complement(23755..24237)
                     /locus_tag="VG272_10890"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWF14232.1"
                     /translation="MDAEADDLTRPELIFQPILLFLFEQGGAMAIERPIEYEDAGEDV
                     RAVYDDIRATRKTDYVTNFWKVLANDPVTLKRTWESTKQIMGPGALDAVTKELIYIAV
                     SVTNQCPYCIASHTASAQAKGMTPEIFHELMAIVGMANETNRLVAGYQVEIDERFKNS
                     "
     gene            complement(24198..24986)
                     /locus_tag="VG272_10895"
     CDS             complement(24198..24986)
                     /locus_tag="VG272_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14233.1"
                     /translation="MKFFQKNLYFVLAWIAFGFFAAPAITFAQTDEIQVYDAEIENQG
                     KFNIMIHNNFTPSGRKTADFPGGIIPNHSDNGAVEWAYGVNDWFEQGLYFPVYSAYST
                     GRGGSLNGFKIRELFVRPHAHDHMFFYGVNFEFSVNYSYWEQRHITSEVRPIVGLHLG
                     KVDLVYNPIMDTDYTGGFKNLEFVPATRVAYNFNDKWAAALEEYSDDGPLRKFLPMRD
                     EFHEVWAVMDHNTKWVNIETGVGVGVTHGADQWTLKLMISRDLN"
     gene            complement(25568..26521)
                     /locus_tag="VG272_10900"
     CDS             complement(25568..26521)
                     /locus_tag="VG272_10900"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HWF14234.1"
                     /translation="MDSAVKQFLNYLRSVRNSSPNTIRSYESDLGQFLLFLTPPGLAM
                     PPPQDVTHLMIREFMAQLHDLRLEKSSIARKLAAIRSFFKYAVREGIVVRNPARMVST
                     PKLPKRIPSVLSAEDLNAFLDGVVERPKRPSSRRKNRGMHDDSHLMVKRDRAILELLY
                     ASGLRVSELTGLNLVDIDRKDLMLRVRGKGNKERIVPYGGKAELSLEAYEPLRQELLR
                     KAGRRGDQQAIFLNHFGTRLTQRSVARIVKKYARLANVNWDLHPHSLRHAFATHLLAD
                     GADLRAIQELLGHSSLSTTQRYTHATIRQLLEVFDKAHPRA"
     gene            complement(26581..29166)
                     /gene="topA"
                     /locus_tag="VG272_10905"
     CDS             complement(26581..29166)
                     /gene="topA"
                     /locus_tag="VG272_10905"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HWF14235.1"
                     /translation="MGRSLVIVESPAKAKTINKYLGRNYVVKASYGHVMDLPKKVMGI
                     DIDHGFEPTYEVNPDKKNVVTQLQKAASTADAVYLAGDPDREGEAICAHLADVLSGPK
                     LIFLTDEEIATEEAKAKEKDKGKGKKGKKKTKTQVKTVIAKGKPVIARKNIYRVMFNE
                     ITSKAIRAAFEHPGEINENLVDAQQARRVLDRLVGYKISPLLWDKVRRGLSAGRVQTV
                     ALRLIVDREMEIRAFIPVEYWTIHAMLDAGVPPSFEAKLTKFKGEDIEVHTQAEADAI
                     VAATGSVEWKVANVTQKEKRRFAPPPFTTSKLQQAAYNRLRYTAKRTMGLAQRLYEGV
                     ELGEEGSVALITYMRTDSVRVSNDALDQVRGLIGAKFGQPYLPEKPNFFKSKKDAQDA
                     HEAIRPTDAARTPEDVKPFLSEDMFKLYQLIWQRFVASQMVPAVFDQTSVDVSAADYT
                     FRASGSVQKFDGYLAVYQVSADDDEEGEADPAKALLPPLTEGEILKLENLRPDQHFTE
                     PPPRYTEATLVKELEEKGIGRPSTYASIISTIVEREYVTKDQGRFAPTMLGEKVSILL
                     VKSFEDIFDVGFTARMEEELDEIEEGKLPWKRAVKEFYGRFDADLEQAKDEMESYKAG
                     IPTGQKCEKCGEGELLERISRHGFFLGCSRYPDCDFIRDLSPELPPEGEDGAAKIEAC
                     DNCGREMIIKRGRWGMFLACTGYPDCKTTRRLKAGTRKALQPDELLDEKCPQCNSQLV
                     KKYGQYGEFVGCTAYPKCKFIRSKTLGIKCAKCETGELVERVAKKGRRRTFYGCNKYP
                     ECDFTTPHLPIAEPCPKCGAAFIVEKRGKFGAIRSCVKEGCDWEAVVSEAPAAAVPEP
                     VGTAQ"
     gene            complement(29390..30517)
                     /gene="dprA"
                     /locus_tag="VG272_10910"
     CDS             complement(29390..30517)
                     /gene="dprA"
                     /locus_tag="VG272_10910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HWF14236.1"
                     /translation="MELEASLDWLSLSMTPGLGSRLTGKLLRQFGTPSGVFRASLTEL
                     EACHLPVAPARAIHTKLAHKDAEAELAQVRTLGCRLLNWSEPEYPQRLLEIYDPPPLL
                     YVRGDISLLNRHSVSMVGTRRPTPYGNQIAERLAHDLAERGLVIVSGMARGIDSLAHQ
                     GACRATNGATIGVLGTGVDVIYPKENKKLFAEVEKRGALISEFPLGTHPAPENFPIRN
                     RVVAGMALGVLVVQGAQYSGSLITARLGMEFGREVYGVPGNVTVDVSFAPNQMIKQGA
                     KLVTSWEDVVEELPTEIRVELFPVEATSKAERASLFEKALSAAEKKIFALLDTDESIH
                     VDELVEKTELNSSEVLASLCEMEMKRMVRQMPGKQFLKILL"
     gene            30972..33182
                     /locus_tag="VG272_10915"
     CDS             30972..33182
                     /locus_tag="VG272_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HWF14237.1"
                     /translation="MINLQMSARAGLISVCLVMAAWKPAASQTAASMNRAKTEKISVS
                     QNDIGGAVSGLNGPEAGVWVIAETTDLPTKFRKIVVTDERGRFVIPDLPKGNYKIWVR
                     GYGLVDSTPIVSAPGKTLSLQAVLAPDARAAAQIYPANYWYSLLQVPSAREFPFLKPA
                     RNSASQRGRRENGQASVLSGGIHNQAEWLFGMRTCTQCHQLGDKFTREIEPSLGTFDS
                     SAAAWDRRLKSGQRGPLMSTALNQLPHDRALTMFSGWSDQVAAGALPPVPPRPQGIER
                     NVVITEWEWGRGPADWVHDEMTTDKRNPTVNPHGLVYATSEGAGTIEVENPLTHESSY
                     IDIPTRVDQKEIAPYLMSLDVGFASPYFGEQQLWTATSSPHNLMMDEKGRVWVTSAIR
                     GKNDPPYCKEGSNNIFAKNDPIDVSIRQLSMYDPETKKWIMIDTCFGTHHLQFAQDKD
                     NTLFFSGEEGAVGWVNTRLYEETHDEQAAQGWCPAYIDTRGDGKFDWKTDNAVSGFPY
                     GLAVNPVDGSAWYAAVGVPGKLVRVDRGTNPPATCHTEVYEPPYESSSIAGNQAFFPH
                     GVDIDSNGIVWTSLAGSGHLASFDRRKCAVLSGPNATGQHCPEGWTMYRLPGPLLKGT
                     DVGSADYPYYNWVDRFDTFGLGENIPIATGTNSDSLLVLQPDTKKWVILRVPYPLGFY
                     SRGLDGRIDDPNGGWKGRGLWSNFSEIPVWHIEGGKSAVSILLHFQLRPDPLAH"
     gene            33379..33846
                     /locus_tag="VG272_10920"
     CDS             33379..33846
                     /locus_tag="VG272_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14238.1"
                     /translation="MAGTFVGQANFRNISWAIDGLGLIVATSLLTIKYFRKGCDFIAA
                     GFLVFAIGESVMLAGTASGLAASAPSFAAGTSMWATAILVVSIPAEFSLWVRLIGFIA
                     SALFYVTATKIFWGAPITPLSSPLPFFAYPFLVLTFVGWIVMLLTENNGGNEA"
     gene            complement(34033..34326)
                     /locus_tag="VG272_10925"
     CDS             complement(34033..34326)
                     /locus_tag="VG272_10925"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HWF14239.1"
                     /translation="MAKGYWICFYRSISDADKVAEYAKLAGPAIQGGGGRFLGRGLPS
                     KTYEAGKSMRTVVIEFESVQQATTTFESPAYQAACRVLDGAAERDIRIIEGLA"
     gene            complement(34503..35630)
                     /locus_tag="VG272_10930"
     CDS             complement(34503..35630)
                     /locus_tag="VG272_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14240.1"
                     /translation="MQFLTGVKVHVGAGRNQELAARVCDASLSWSDQTLALTSAASEL
                     DIDALGVDLGVGAPVLALQVKKLKTECCMAYEIYSLKQPPMLLRRIAGGEYFSAADTD
                     LDGRVEIWTDDAAAVEGFENLRLSDLDFAPPIVLRFARGRLLDVSSEFRPYYNQKIAE
                     ERAKIVPEDLDDFKKSDGKLDPASMIPVVKLSRMRSAKIKVLEIVWSYLYSGREPDAW
                     HALQEMWPASDIDRIRSAVSGALARGIRSQVDGVLTVTHPGRQSYAKIFDGTVVVSAP
                     PGLTPKNAKVAPPITPPRAILMEREPPLTAMEQELAKNESTLKLVIDSAGKVRSVEVI
                     GNVEKVDEQLLRSTTGWKFIPAFNADQPVASQILLGVSLRR"
     gene            complement(35923..37020)
                     /locus_tag="VG272_10935"
     CDS             complement(35923..37020)
                     /locus_tag="VG272_10935"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF14241.1"
                     /translation="MKNRRDFLKTVAGATTGALLTGNTFAHAALQGAAATAAPVKRRE
                     VSIGGKRIKVVDVHAHCVIPEVWDIVKDTPLAPNAGPRARGSNLMGLDRIRAIDGLGI
                     DVQVLSINGFWFYGADRDLAAKIVKLHDEKLADWVNAHSDRYVGVSSVALQFPDLAAE
                     QLEYAVKKLGARGAAIGGHVQGEDISLPKYDPFWAKAQELGVMVFMHPQGAENVIKQD
                     AWKGRGDLGNIIGNPLETTIFLTRMIVNGAFDRFPNLKICAAHSGGYLPSYLGRTEIA
                     CEVRPNADCLNKKHPSEYLKTQVLVDTIIISDEGLRHLVAEEGVGQVVFGTDMPFNWH
                     SNVDLVLNAPFLSDAQKEAILGGTLVKLLKL"
     gene            37223..38125
                     /locus_tag="VG272_10940"
     CDS             37223..38125
                     /locus_tag="VG272_10940"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HWF14242.1"
                     /translation="MGKLRISIVEFLNTAPLVWGFTDGPLTGKYDLSFAVPSQCADDL
                     RAGRADVGIIPSIEYQRMEDVVALPGMAIASKQEVRSLLAVSKVPIEMARSFALDTNS
                     RSTVGLTRILSRRHWNIDPEFIDMPPDPDKMLARADAALVIGDPALRLRLEIDALQAK
                     TPSGEDCCCCDGEDEHPVKGIETLFVYDMAQQWREMTGLPAVLAIWVARRGVITPEMA
                     VDFQASREYGLSHIGDIAEGAALKLELPPRELELYLTENIDFSLDDENLAGLRRYYDE
                     CALAGLIPKARDLEFVGGMLAAEK"
     gene            38356..39390
                     /gene="mqnC"
                     /locus_tag="VG272_10945"
     CDS             38356..39390
                     /gene="mqnC"
                     /locus_tag="VG272_10945"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HWF14243.1"
                     /translation="MGLTQQEALDLFRSDDLVGIGMAAMEVRRKKTDPRVVTYQIDRN
                     INYTNFCTEYCSFCAFYRPLGSKEGYLLSFETIYKKIEEMIELGGTGILLQGGLHPDL
                     RVEYYENLLRSLKERFPQVHLHCFSAPEIGCIAEVSGLSVREAITRLMAAGLDSIPGG
                     GAEILDDEIRARISRLKCTSDEWEDMHRVAHSLGMRTTATMMFGCGEEFRHRVNHLER
                     LRRIQEDTGGFTAFIPWMFAPDHTPLGKKVPEATAVDYLKTLAISRMYLDNIDHIQSS
                     WLTPGIKVCQVGLQFGADDVGSILIEENVVFAAGVRNRTNEGELRRIIEDAGYRPYQR
                     DTLYRSYSLK"
     gene            complement(39536..40555)
                     /locus_tag="VG272_10950"
     CDS             complement(39536..40555)
                     /locus_tag="VG272_10950"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HWF14244.1"
                     /translation="MIKPTHKSSGHLFDEPEIPSTRQEGAYSANIDGAARGNPGPAAY
                     GVIIKRPDGKKHESLGKYIGRTTNNVAEYYALIAALDYAAASGIRRLRVYSDSQLIVN
                     QIKGIYKVKHPDLRPLHERAKKQAAGLESFTIQYVPREENRDADEMANAALDSTNGVK
                     PAYGSGTAATSNPAAAKIAPPLAMSAPAAKALYLEDLHVGQKFTSGAYQMDEERIKSF
                     AAEFDPQPFHLDEAVAQQSVFRGLSASGWHTAAATMHLMVTGGLPFATGLIGVGAEIE
                     WPRPTRRGDTLRANSEILEITPSRSKPNQGIVKVRVITSNQNSEPVQIMTTKILVFKR
                     TEALA"
     gene            complement(40574..41362)
                     /locus_tag="VG272_10955"
     CDS             complement(40574..41362)
                     /locus_tag="VG272_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14245.1"
                     /translation="MHPHLKPLIELQAVDLRLIGIRERLAQFPKRLAEAEARVAAAKH
                     EVAVAKEALLTGQKERKTFEMDVEQWKERAKKYRNQTGEVKSNEAYKALLHEIQNAED
                     EIAKAEDRLLERMVSGEQYDREVKAAEIRVKEVEASAAKERQAIQAEYNATQKELGAA
                     EAVRAADMAAVPEDLVDHYERIAKRHGGIALAEIRGEGCGQCGVHIRPHVIQQLQREN
                     NDAFFHCETCTRILYYAASPELSANSASATASAGDAPNSPAHEK"
     gene            41519..41716
                     /locus_tag="VG272_10960"
     CDS             41519..41716
                     /locus_tag="VG272_10960"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HWF14246.1"
                     /translation="MPDVHILWLAGRTVDQKRKVVEGITKVLAEDAGAKPESTHIVFV
                     DIPHTDFASNGMLYADKNKKA"
     gene            complement(41804..42214)
                     /locus_tag="VG272_10965"
     CDS             complement(41804..42214)
                     /locus_tag="VG272_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HWF14247.1"
                     /translation="MKGIFGVLGLLITAAVGMGIYSYTLKQAAPAPGMTVTQNISLTG
                     VKMDLTSIAQAERLYFTQNGSYTDLETLSSSGTMNITRTNRDGYAYSAQASGDSFTVT
                     ATYTAPPVSNPTGVTPPHFPTFTIDQSMEVHQID"
     gene            42515..43708
                     /locus_tag="VG272_10970"
     CDS             42515..43708
                     /locus_tag="VG272_10970"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HWF14248.1"
                     /translation="MPAIFPGPSIHAASRDTAVSVFATSIVGKQVELNGSPMSAGATL
                     FRGDVVRLGENSTAALQFGNSSVLAAPLTELVVEAQSLKLRNGRLQVRVNGKEPFTVS
                     GSFFHLNIAGAEGIAGSAEIRLGGAHAEVSAIAGITELTPAGDVPYKLQPGETATFDE
                     SAGQPAAGQSTTNPAAGQVSRLIPQVQIDRASQHIVAEVSSGVFWNDDLRSGPTGRAH
                     ITLKDGSQLNLGSDSNLRIVQHDAQAQQTSLDLLMGRVRGRITKLSKPDAKFEIHTPV
                     GVAGLVGTDLSLLVTNDFTELMVFEGTVRFTTLNGQSVTVTAGNKIRISKAGALDGPL
                     PFAAPEAQTAQNLTDITGGANSAPVMAAARPTVPMIITLTGAAAGVGIGVWQGTRPAV
                     SISVP"
BASE COUNT         9922 a        11877 c        11983 g        10232 t
ORIGIN      
        1 cgaagaccgg gaaaattacg tattacaaga ctccgactcc gaattcagct ccacgtcgtg
       61 gctcggtcga ttcagcaggg cacatttggt tcggcgaata tcgcggcgac cgcataggaa
      121 tgttcgaccc ggagaccgaa aaatttcagg aatggactcc gccgactccg tgggcgctac
      181 cgtacgacgt agtcgcagat aagaacggcg acgtatgggc cgggtccatg gtgaatgacc
      241 gtgtcacgcg cctgaacccc aaaaccggcg atatgacgga ctatctgctt ccgagagaga
      301 cgaacatacg acgtgtgttt gtggacaatt ccacaacgcc ggtcaccttc tgggttggga
      361 acgaccatgc cgcgtcgatc attaaactgg agccgctgga ctagggcgcc aacggcttga
      421 ggtgtcagga atagatctcg aaaaatattt cgtaggcctt gtcgacgatc atgaaaactt
      481 tgaatccgcc tcggtatggc ttatgtaacg cgatagcccc caattcgaag ggagcggccc
      541 caaatccttt ccaccgggct tataatagat agagattgcc cgagaggctg ccatgacgat
      601 tgaacagcag gttgttgaaa aactccgcga tttgcctcct gagaagcaga aggaagtgtt
      661 ggactttgtg gacttcctga gagagaagaa tggcggcccg aagaagcctc gccgaagtct
      721 gcttgggttg tgggcagacc tgaatatcca cattactgag gaagatatcg ccgacgcccg
      781 ccgcgaaatg tggggtaact tcccgcgcga catcgaacta tgagcgcggt gttggatact
      841 cacactgtcc tttggtattt ggaaaactcc aaggaattgt ctggggttgc ccggatcacg
      901 attgaagatg ctgtccgcga cgctcgcgac gtgcacgtat cggcgatatc cctcgttgaa
      961 acggtctact tggtggaaag acgaaggctt ccactcaacg cgttggagcg gcttcgttcg
     1021 gctcttaatg acccaaactc aggactgttc atcgccccag taaatggtag cgtggcagac
     1081 gcgctaccga aaattccccg cgacgttgtt cccgacatgc ctgatcgcat catcgcggcg
     1141 actgctcttc acctcggcct tccgctcata acccgcgacc gccgcctcca atctgctgga
     1201 atccaaacaa tctggtaaca gctagttgtc cgtcttgagc tcgatatcac ccttgccgat
     1261 attgcagacg ttgcagagga tctgcagatt ttcgatcacc gtctcgccgt ccttgctcca
     1321 tggctttacg tgatcgacat gcagagtcac accctcgctt ggtcccgctc cgcacagccg
     1381 acagcgtgcg cgatcccgca tgaggacaag cgcacgcaac ctccagttga tgtttcgagg
     1441 tgtcctctta ccggatttct tcgcgctccc ttcggagtcg ggcacaaatt taccatcgtt
     1501 ggcccaccga acaaactctg cgagcgcctt gcgccatgtg ctaaagcggt acgcataagt
     1561 ggcccatgag aatcgagagg gaccgggcct caaatcccga gaccgaggct gccgcccgag
     1621 ggcggtccag acttctacca gatttacaaa aagctcttcg tccgaaatat ttcgattcat
     1681 cgtccgctca aggttagcct tgtcaatcgc ggcaaaccac gaaccgaagc gctctgccaa
     1741 cgttccagca gtaaaacgcc ccctttcgtt gtactcgcga taggtcactc ggttcttgcc
     1801 caagtctttg gcaacgcggc ggagatctgc taacaggtct tcgtccggaa tgtcacggtg
     1861 gaagggttct agttcgtata tcactgtggt gccaatatag aactacactg gggatgcaac
     1921 aagcgtttat tccgcctacg gaagcccttc gacatgatct tcgagaaatt aagaacggta
     1981 gaatggtggg cctgggtaga cttgaactac cgacctcgcc cttatcaggg gcgcgctcta
     2041 gccacctgag ctacaggccc aattgggaag caaccgctgt tgcagtacaa gcatgatagc
     2101 ggtcgagcaa cgaatgggtc aagtacagag cggaaaatgc agataaataa agcctttatt
     2161 cggcacgcct gaaaggcaga catgatctgg tattttctca accaggcccg ttccaaaggt
     2221 gtatattgcc gggcagccga tcgtccaacg cggaaattta aatcgtaacg actctagttg
     2281 agtctccagg aggctgcccg tgaaattgat taagcccaaa tttgtggtca ttctagtcct
     2341 aacctttctc acgctctttt cgtccgctgg aaatgtgcga gcacaggaca aaaggactgg
     2401 ccgttcaagc ggacccatcg ttacagggaa agtgtatgca tttgaaaaaa ttgccgacgg
     2461 cgtttattac acgacctctg atagtccgat ggcgacgggc ggcaatcaca ccataattgt
     2521 cggcgatcaa aatgtatttc ttgtggatgc cgggacaact cctgcggctg cacgagcgct
     2581 cctcgcggac atgaaactga ttacggacaa gcccgtccgt tgggtcgtga atactcactt
     2641 tcattttgat cacacggatg gcaactcgat cttcggaccg gacgtggaaa tcatcggtca
     2701 cgagtatgtt cgccacatga tcgcggatga ggatgtcctg catatcgagc cattcaagac
     2761 ggcgatgatg aacatgccga tgcaagtgaa agcgctgaag aaacagattg ctgacgaaaa
     2821 agattccgcg aagcgcgcca ctctggaagg gcagctcgct gctaagcaaa cggattggga
     2881 agaattcaag atgctgaagc ccactccgcc gaacgtaacc tacagctcaa aaatgacggt
     2941 gtttcagggc tcgcgcgaaa tccaattgct gtttcttggg cgcggccaca cgcagggcga
     3001 taccgttgtt tacctgccga aagagcgaat cgtttgcagc ggagatatga tggaaactca
     3061 gccggcctac atgggcaatg cgttcttcga tgattggatt gccaccctgg acaagctgaa
     3121 agaaatgaat ttcgaaacca tgctgcccgg gcatggcgtt ccgttccacg acaaggctct
     3181 gatcactgcg tatcaaagct acttgaaaga tttaatgacc caagtggcgg cacttcgaaa
     3241 gcagggagtt tcggccgacg atgccgccaa aagagtcgac atgacttcgc acaaggccga
     3301 tttcccacag attcaaggcc cgggcgcgga agtccgagga gtgcgtcgaa tgtacgaatg
     3361 gatggatgga cagtcgcgct aaggaatgag aagtgcatca tttctgaaaa tgcaaagcca
     3421 acgacgtttt gatggcgccg ttggctttgc ctacggaaat cattcgaacg aatacattcg
     3481 cgttaagact ttactacttg ctccagacca tcgtgttatc gaaatctccg gcagggctgg
     3541 ttcccttggc atggatcgtc aaagtcttgc cgccgttgga aactgcgaag gttgtgagtt
     3601 cgacctgttt tccggccttg aaaaatttgg cttcgtacga actggcgtca actttcttgg
     3661 aagtaatggt atccgccgtg ttgggaatcg cgccaactgt gggatattcc ttgccatcac
     3721 ccttgatcgt gtaaccgtag gacgatgctt ttcctgtcgc gcctacggaa tcgaccgtaa
     3781 ccttgcccga ggcttcccac gtgcgtgtct ggctctgcgg agccggtcca ggattatatt
     3841 tcgagctcgc cacatccagt ttccatgtgc ctacaaacgg attcttttgg gccagcacca
     3901 cgcttcccgc agtcagcaca actaaaaaag ctgcaaaaac cgctactcgt cgcttcatca
     3961 aacatcctcc ggaatagatt ttttctgcaa atgtgatttt ttcggaaaac agtatgcaaa
     4021 aggttatgtc gccgaacatc ggtagtcaag ttatgccgga cccagggagt cctaatttaa
     4081 aaatctctcg aatttaggat ggccgcgttt taactcgacc gtttgggttg cctttcgtcg
     4141 caggcttcag cgcctgaagt ttcagggatt aaaactccga tcaaaccaag ctcctcgtcg
     4201 ggacagaagt cccgacctcc taaggaaccg tgccacgcga attcttttca aattagggca
     4261 acacccaggc cggcttgtgc ttgaggcgat gaagccggta gcgtatcatt cacaacggca
     4321 cgggacggtc gaatacaaga gatgatcaca cgaaaaattg gcgagggtgg ccgctggctg
     4381 ttagaaaaaa ttgtcgatgc agtcgccgca accggcatta atccaaacct cctcacgttt
     4441 ttcggcctat tggtgaattg ctgggccgcc tatttgtttg ccatcggaag attccggggt
     4501 ggcgccatgg tgattttctt cgcagcgttt ttggacatgc tcgacgggca ggtagcgcgg
     4561 cgggaaaaac gcgtaaccgc atttggcgct tttttcgatt cgacgctgga ccgctactcg
     4621 gacatggcgt tgtacatggg cttgctggtt tattatgcgg tctcgcagcg gacctcttac
     4681 gttgtgctct ccgcggttgc gacagcggga tcggtaatgg tcagctattc gcgcgcgcgc
     4741 gccgagtcct tgattcctct ttgtaaagtt ggcttcatgg agcgtccgga gcgcatggtc
     4801 ctgttaatcc ttggaggcct gtttaatcgc atggcccccg cgctctgggt gatcgcaatc
     4861 gtcgccacac ttacggtcat tcatcgaatt gtccacacgt ggcaggaaac gaaggcagga
     4921 cgcacgcttc cgggaatacg tctttcgctt tagtcattcg actggccgct gttttttctc
     4981 tcaagaaata actagacgag gtccttcagt ttttcggtac gggcgcgagc ttgttcgata
     5041 ggttccgcga cgattgcatg cacgcggtca cgaagtgctg ggaaatcgtt cttccgcaaa
     5101 tcttttgttt caatcacgtc atgcaaatgc acggtcacgg tgcccggccg aatcaaccag
     5161 cttgtctttt tgttgaattc aaacgcgccg acaaagctga cgggaacgat gggcgtgcca
     5221 aattgcagcg ccatgcggaa cactccgtcc ttgaacggtc caacgtggcc atcacgtgtt
     5281 cgctgccctt cggggaatac ggcgaggctc acgcctgaat cgagcgctgc ccgcgtcagc
     5341 cgccacatgc gtttcaagtc cgctgggcgg ttgacgtcag gtacaggaat attgccaaag
     5401 cgtttcatca tccagccata caccggaatc cgaaaatgag attcgagttc cagtccacgc
     5461 aaaaattgcg ggatcgtggc gtatagcagg aatggatcaa atagattgac gtgattgacg
     5521 agaaaaaaac aggtgcgtgc gggatcgaag ccgggagagc gctgcacgac aacgttgact
     5581 cctgcaagct tcatgacaac gcggcagagc aggcgctgag cgccatcgtt cttgcgcgga
     5641 tcgatgaaga ttcccagcag aacgagaaag ctcgcgacgg gaaagaaaaa tgccgcggag
     5701 agcagccagt gataggtgct ccgagcaatg gcccaggcgc gtcggaggcc gtgctcgccg
     5761 gcaagctccg tcgctgcagg gaccggaatc ggttgttcgc tttctccgtt cataccatta
     5821 cgttgagcgc gccgggaagt acttccagcg attccgcctc gagcgtgagc acctctccat
     5881 cgacgaccac gtcgacagga ttcttcagat caaattggat gcgcgtcgcc gttcgcctcg
     5941 aggccatagg atgccgaatg tgtgtaccgg taaacagagt cggcaaattt cgcactaaac
     6001 tcaaacgccc gatgggcccc caatgcacat attcaattgc accgctgtcg atggaggctt
     6061 gcggcgcgat catcatttta ccgccagtgt acttgctatt gctaaaagca acgaagagac
     6121 agcgacggtc gtcccaatcg ccatccgcgc ggagcgggaa tgggcggcgg tgcagccggg
     6181 caagcgaaac gagcactcca accaaataac cgggctcgcc caaaaatttc agatgacgat
     6241 tcgtcagcgt tgcaacgtcg gccgcgaatc ccatgttcag cgtattgatg taataaatgg
     6301 agccgcccga atgattcaga cgaatgacgt cgcagggacg cgagcgcctt tcgagaattg
     6361 cattaatccc aaattctgcc aggtccgttc cgttttgcac gaagtctcga agaaacgaat
     6421 ttccagttcc gaggggaaga aatccgaggg aagcgcgtcc ttcgatagcg gcctccggaa
     6481 atattccgtt aacgatctca aaacttgtgc cgtcaccgcc gaccgctatg aatctgcgga
     6541 atccatcggc gtatgcaccg ttcgcgtata ccgttccctc gccgggcgct cgagtttcgc
     6601 gcacttccag ttgcagtcct gccgcgcgca gacggtggag cgctttagga gccagctttc
     6661 cacaacggcc gcctcctgcc gcgggattca caatcgtgaa atacgcttcg ttcacagggc
     6721 gaccattgcg ctgcggtcga gttggtcgcc gatttgcccg gcaaggatcg gacgcctgat
     6781 tttcattgcg gcgttgcgcg tgaaatcctg atcccaaacg atgtagcccg ctacgcgttt
     6841 gtaattgaga agctgtgagt tgcgcctgga aagttctttc accaggaaat cgttcacggt
     6901 ttgtcccggc tcgggatgga ttacgaggac gagttgctct ccggtcatcg tgtgctgtgg
     6961 ccagatgtaa ttcgcggcga aaacacaaaa ctctttcgcc gcgatgccct caaaattatt
     7021 ttcaatgtct tcgggataaa tatttttccc ttcggccgtg acaatcatgt tcttcttgcg
     7081 gccgaaaagc tgcaaatgcc ccgtggcatc gagacggccc aaatcgccgg tcatcagcca
     7141 gccatttacg atggtttcgg ccgtcatttc gggatcgtcg agatattcgg acatcaccgt
     7201 cttgctgcgc acggcgactt cgccgattcc gtcagcatct ggctgcgcga tgcgaatttc
     7261 cattccgcgc acgggtttgc ccacagtatc aggccggaag ggacgcagat cgttcagcgt
     7321 gatcgccgtg cacgcttccg tgcaaccgta gccattgtaa atagggatgc cgatgtcatg
     7381 aaaaaactca atcgtagcgg gctcggtgaa tgcgccgccg acgagcagcg cttgcaggcc
     7441 gcctccaaat gctttgtgaa tttcctttag cagcgtacgg ctcagcccag ggcgcggttt
     7501 gccgcgcgta agcgcgcgat tcacgctgat caatgcatcc aacattttgc gttttccaga
     7561 tggcagcgct gcgaatttct cgcgcaagcc gcgctgcagg tttttcaaga tcagggggac
     7621 gacggccatg tacgtaatct tatatttagt aaaggcttcg cggatgaatt ccggacgaag
     7681 agtgcgaagg tggacaactg ttcctccacc ggtgaagggc atgataaatc cgcccatgaa
     7741 atcgatcgcg tgattggtgg ggataatgct cagataccgc gcgccgggcc agaatggaaa
     7801 aacaggtgtc acggcggcac actgttccag ataattatcg tgcgtcaaca cgcatccgcg
     7861 cggatggcct cccgtgcctg acgagtacac gacgcaggcc gtgtccttcc ttgtgcgcgg
     7921 tttgaattcc ggcgcacacg tgccgcgaaa ttcttcccag cgttgcgcgc ctgcgaggtc
     7981 ggcattttga ggcgcttcgc tcacgagcac cgttttcagt ttgtgttcgg caaacgcggg
     8041 cgattgagta atggcgcgcc acagcgggta ctcgacaaat aaaatttgcg ccttggaatg
     8101 tgccagaagt tgcagctgtt cgccagcgga gagtttgtaa tcaagtggga caagaatgcc
     8161 gcctgcgaaa aacacggcgt aagccgaaat caaccacttg ggctgattgg tcatgatgat
     8221 tgcggcgcga tttcctgctg caaagccgga ttcttcgagt gctgcggcaa tcggttcggc
     8281 ggcttcgccg aattgcttgt aggtgaggcg ggatttttct ttctcgcggt ccgcttcgat
     8341 caggcacact tcatccggcc agcgaccgag cgcgtcgcgc agcgccgctc ccaccgaatc
     8401 gtatttattc aaatcaagca tcagaattgc catagagaca cagagccgca gagagaagca
     8461 agattatagg aggaaatcat tgaatgaaac gttttattcc ttcatgtagt agacgggaat
     8521 taaaattgac gataagtccg acatgcgttc cggtgacacg cagatatgtc agtatttgcg
     8581 cctcaaaaat ccggtcaaac ctttctacgc tttcaatttc aacgatgact ttgttctcta
     8641 ccggcatgtc gaggcgatat tcccctattt ttcgaccctt gtaaattacc ggaaatatta
     8701 cttggcgctc gaagagaatt cccgcatctt ccaattcaat agccagagct gcttcgtagg
     8761 tgctttcaag aagacctggt ccgagttcac gatgcgtctc aatggcgcaa ccaataatct
     8821 tctcggttaa tttgttgatg tctggcatcc tagcgctgca aaattcaagg gacacttgcg
     8881 gttaccttgc taacaagaac taatttttct ccgagtctct gtgcctctgt ggcagatcag
     8941 agccgtgcct gaatgcgcgt tgccagagtg tggaagtcgc ttacgccttg aacttcgtaa
     9001 tcgggaagct ccacaccgaa atgattttcc aggcgggtga gcaattcgag cgcctgcagg
     9061 ctgtcgatgc ccaacttctt ataaaaatcg tccgtcgccg ccaggtccgt gcgcgggaat
     9121 ttaaaatgag tcgcggcaag attcaggatt tcgtctgttg tttcctcaat tgttttcatg
     9181 atcgacccct attttacagg aagcctgcgc ctgctggcta ttctccgagt atttttgtag
     9241 gacaactccg catcgctagc gtgacgaaac tggtcatggc aaatacggat gcgctcccgt
     9301 attttcaacg aacttgcgca aaccttccat gacggctggg cgcgtaaaga ggtcacagaa
     9361 aatgtcgatt tcgcggcgca attcttccgc ggggagcggc ttgatgaact gcttcgcggc
     9421 cgaagaggta ccgcgatcga attttgtcgt ctgcgctgca gtgaggcgcg cgacacgcaa
     9481 cgcttcgcct tccgccgtca cttggctcac gaggcccaca gaaagtccct tcgtggcgtt
     9541 gatgctccga cccgtgagca aaagatcgcg caccacaccg ttgccgatat cgcgtttcag
     9601 acgcggaatt ccgccgaagc cgggaatgag accgagccgc aattccggaa aacaaaagcg
     9661 cgccatttta tccgcgatga tcagatcgca cacgagcgcc agctcgaatc cgccgccaaa
     9721 acaaacgccg tgaacggcgg caatcgtcgt aagcgatgac gtgtccagcc tgttgagcac
     9781 cgcgtgaatt cgttcgagga atgcgcggac ggctttttcg gcggcggatg tgtctatttt
     9841 ttgggcttct tcatataatt cccgcaagtc tgcgccggca gagaagccgg cttttagttc
     9901 gctgtgaaga atgagagtgc tggccttcgc ttcaagagcg ggaagcgccg cgacaaaacg
     9961 ctccaggtca tcgagcatcg cgaggccaat ttcattggct ggaggacgat tcagcgcaag
    10021 ctctatgacc ccgtccgcga ggcgccacga aagtgttttt tctttatatc cgtccattgc
    10081 gttttcaacc ttctgcgatc tgcaatgtcg cagcaattga ttctgtagcg cctgcatctt
    10141 gccggcggtt ttagaggcgc gcaaactgcg gcaaaaaaaa ttcaaaatca aaacctgccg
    10201 gcggaacgcc ggcgctacaa aatctaatct ttttcgtaca tgcgttcaat ttccgctgta
    10261 aagtgcgcgg cgattacatc gcgacgcaat tttccgtttg ctgtcagcag accgttttca
    10321 atcgtaaaca attcctgcgc cgcatgatac ttgcgaatct tacggtaatg gggcaatgcc
    10381 gtgtttattc tctctagctg cgaatcgatt tctgcaccag gcgtccccgt aattattgcg
    10441 gccagaaaac tgcggccatt gccgacgagc atgacgtgtt gcgcgcccga aatggcgcgg
    10501 aatagttctt cctcaatcgg ttccggcgca atgttgtgcc ccgaattcag gatgatgaga
    10561 tttttaattc ttcccgtgat gcgccaattt cccaccgcat caacttcgcc ctggtcgccg
    10621 gtatggaacc agccatcgcg caggccggtg gctgtttgct cggggcggcg ccagtacccg
    10681 ggaaaaatat tcggtccccg gacaatgatt tcgccgtcgt cagcaatttt catttcgatg
    10741 ccgggaatcg ccggaccaac gcggccgggc tcgacatgct gtggatcgtc catcgtgcaa
    10801 atcgcagtgg tttccgtcag accataaacc tgcagcacgc ggatcccgag catcatgaaa
    10861 aaaagctgag tttcacgggc cagtggagcg gagccgcaaa tcagcgcgcg gagattgggt
    10921 cccatgcgtt tgcgaatcga tgggaaaatc aaaagacccg caaacccgag ccagaaaaag
    10981 tcccacgcat ggggcgcttt ggcatccagc ctcagccatg ccgcattcgc gttttcaaaa
    11041 atcctggcaa tcgcgccgcc gcgctttcgg atgttgtccg cgacgccagt gcgaatgcgc
    11101 tccaagagag ccggcacgtt cagaaaataa tccggctcgg ccgcgcggat ttcatccgca
    11161 agcttcgtaa gatccatgga catggtaaga acgctgtttc gcgacaggca cgacaacagc
    11221 aaaatccacg atcccgcaaa acagaagggc aggtaatgaa acacgcgatc cggcacattg
    11281 cgggttccca tcaaattatc gaggcgcgat ttggtgcagg aaagcatgtg atcgagattg
    11341 ccaaccgtca gcatcacccc tttggcttcg cctgaagttc ctgacgtgta aagaatggca
    11401 accgtgtcct gcggtgaaag ggtggcaggc ggaagaggcg cgattgttgc ggccgatgat
    11461 ttggaattaa aaatatcgtc gaacaaaaca aatcgcggag cctcgggcca ctgcgccgaa
    11521 atcgcatcgc gaagcgtgga atcaccgcag cagatcagag caggctgcgc atcgcgcagc
    11581 atcgccgcca gctcaccggc tgcctgtcgc gcgtacattg gaacggcgat gagtccttcg
    11641 gaaataattg caaggtcgag cgcagcccag cgaattccgt tcggtgcgac cagcgcacaa
    11701 cggtctccct tcgccaatcc ggatgcacgc aagaaggcgc gcgcggcggc gatttgcgcg
    11761 agcaaatcgg aacccgtcgc ggtgcgaacg ccgtctgcgt gcgcttcggc aaggaccaca
    11821 cgactggaag attcctgtaa gcgtgcgaaa atgttttcga tgaaattcaa gttgttttat
    11881 gtggcatcgg cttcagcctg tcggttttgt tccttgcaag gactaccccc caggctgaag
    11941 cctgtgctac tacatcccgc ttaacttgcg gccgcgctga cagcagtgtt ttcgtaagtg
    12001 tctaataact tgccaaggtt cgggctaagc gggggcaaat actcttccca gccgcggacg
    12061 ccggaccgcg tcggaaatcc gggatagcgt tcctgcacag cgcgctccag gtacatgccc
    12121 atccgcgcca tggattttaa attgcgcacc acgcaacggc cgattccatg gcgaatattt
    12181 tccgcatcgt tcgagagatt ttccatcacc gcaaaaagtt tggcttccag atcagggtcg
    12241 tccacggtga gaagtaaatt ctcgtgtccg cggtcacgaa gtaaattttg tatgcgttcg
    12301 tccatcgtga ttccggcgga gggaacgagt cctggcatgg atgtcaccat ggcgtgatag
    12361 cgcgaagaga cgatcatgct ggaaccgcgc agaatgctca cgagttcata catgtcgtgc
    12421 gcatcggacg taaaaaccgg aacaccgccg agctgcggcg tgagtgcgtg gcacgctctt
    12481 gcgtcgaggc gttccatggc aaccagtatg ggaaatacat tttttttcgc tcgaaacgct
    12541 ttcaccgccc cggcaatgga cgcgagatag tttgcgtatg cagcatccac cgcagggccg
    12601 gcgttgtgga aatacacgga actgtaatgg ctttctttat gcgcgccgaa aagtttccgt
    12661 ccaaaccatt tcgtaagtga aggttttacc ggccaccaga acggattgat ggggcacaag
    12721 gcgaggacag gcgtccggcc atcccaaccg gcatcggaaa gagctttatg tccgaaatca
    12781 gcgggacgcg gctcgaaggt ccatgccgtg tctgttccta attccgtggg aatgccaagc
    12841 ttgctgaggg ccgcctgcga ttcttcgttg cgcgtgacga tcaacgagcg gcggcaataa
    12901 tacgcgcaca ttttttcgag catagggtcc atggcgccag cttcgccacc atagcctatc
    12961 gaaagtttat tttcagcgga ggcgattcct agcgctccga tcgccatggt tgtgagagcg
    13021 ttggcaaatt tgcttttgaa catggagcct tcgcacgtaa tcactccatg atgtttacgg
    13081 acttcgctga acaaaaaggg aggaaaaatc atgggcaatt gcacttgcgc ggtgccttca
    13141 aaatagccgc gtgagcgatt cggatcgaaa gtcatcacgc tcagcgcgac attttcctcg
    13201 cccaggatgc gccgcacctg gcgcagcatt tcctcgacgc ggacgtctga cccggtattt
    13261 cgcgttccgt tatatcccgc aaaaagaagt ttcagtttct cgcccggctg ccagcttcgc
    13321 cccgcgccaa acttccagag catgcttgag agttcgatat acgaacttgt ccaggcctcc
    13381 aggatgaaat ccatcatggg tgggagcctc gtgcggagac tgcgctggct gtcgtcacct
    13441 tctcggtttg taaatcactg ccggctgtcc acgccttgta aggataagag aaatgatgat
    13501 cgcggctgaa ttgcagcagc ggatagcagt acttggaaaa cggttcggga gtacggccca
    13561 tttccgcgac gacgcgggta ttgtcgaaga ccgtgtccca aacgagatat ggcataaaca
    13621 ctttcagcaa cgaagcgcta cggccaattg tgccgcgcga attcgatagc atgtttacga
    13681 cagccgaaaa gtttccttcg aggccaggcc agaatagcgg ttccattttt ccggcggact
    13741 tagcaagagc gtgcgtcagc tggccaaatg tttccgctcc cgtgcctgaa gacaggtggt
    13801 aaatctcatg ttccggattt ggcttttgat gcagcatcgc caccgattcc gccacgtaat
    13861 ccactggaac aatgtcaatg cggtcggtcg cgcgaaaggg cagcagcggc agccccgcga
    13921 gaaatacgaa tgcgcggacc atatcgaatt gcgtggtttc cgcgcgacgg ctatcgccca
    13981 gcacaatact cggacgaaac acggtgcgag gaacatcggg aagaagctcg cgcaccatgt
    14041 gctcacagaa ttttttcgtg cgtgcatacg ggtcgtaatc ggagcggttc caatcaatgg
    14101 atagatcttc tgccacgact tcgtggctac gatggcccgc gaccgccaca gtcgaaactt
    14161 ggctaaatct gcgcaagcca tgtgcgtcct gcgagcgccg cgcaagttgc gcaacttcca
    14221 gagtgccgcg caaatttacg ttgaggcagc tcttctcgga tttgcggttt agcgacgcgg
    14281 cgcaatgaat cacagactcc gttgtgccga tcagatgcgt gtagtcagcc gcacccagtc
    14341 cgaaacgcgg gtccgtcaga tctccgcgaa aaatggaaat gcgtgtcgcc agcgcatcgc
    14401 ggaattgcgg aaagtccatg tgcaattgca ttgcgtgcca aagacgttca ctggcttcct
    14461 gttcagtttt cgcgcgcacc aataaattta accggtccgc gtgacgttcc agcagaatgg
    14521 aggtgacatg cgcgccaata tatccggtcg aaccggtcag gaagatggcc atggattagt
    14581 tttgaatcct tgtgcgcgat tccgcacttg cgtgggggac atttactggg ctttgcactc
    14641 cgtcctcgtt gtgatgaagc gtaaaggagc ggtgcggccg aacttccatc gggcggccct
    14701 cgatgagcgg ataaacccac cgtcgtaatg ccgggacgtg aatattaatc caataatccc
    14761 accaatcgac ggaagaagga tcgtaaccaa aagcctttct ttcgtcagcg ggaagggccg
    14821 cggaaagcag ctgcacattt tgagattcga acagctgctc gttgtgcagg atgaacggtt
    14881 cgtagagttc gatcaatttt tcgacgcgaa cgaggtcgcg ctccgccttg gccagcggag
    14941 gctttttcat ttgcagcgcg gaagcgaagc ggttgatgcc gcgcacaaca gccttttgcg
    15001 ccggaacgga aaatttctcg tagcgcgttt tggaaacggg aatcgtatcg aactgcatgc
    15061 gcatccagtt ctcgaggctt tgctgagagc ggtaatgttt gcgatgcgcg aggcccgtca
    15121 gttcaatgga ccgccccatg tcgcacggat tgctcgccga agttgccagt tggtagatcg
    15181 gctcgtgacg tcgcgcgata atcgctgcgg cgatcagagt cattccgcgg cacaccatat
    15241 cgaccggaat tacgtcgagg catttgcgct cgttcgttgg cagttgccga aaataagtgc
    15301 cgagcaagta agagagcggc gcggaagtgt tgatgccctc attccagccg cgaaatggct
    15361 ggtcggtgga tgtttcaaca atcgatgggc gcacgatggc aatcgggagt cctgcgccgt
    15421 gtttcacgat catcgactcg gcgagggatt tcgacagagt gtacgtgttt ggccagccga
    15481 gacggcgtgc gcgacgagtt ccggcgcgcg tcagccggtt gcgtaaccag cgcatacgat
    15541 ttttgcgaat tagattgtcc aactcaggcc cagccggttc tacgctgccg cgatgtttac
    15601 ccagggcttg ccgtcgcaga gccgctgtaa tctccggact ttccgtgcgc gcctccgcgc
    15661 gtgcgaccac ttcctgcagg gagtgccatt cgcgctcggc atcgaaatcc tcgactccga
    15721 gcggcgtgta attcggttgc acttcttcga caacgcgccc gtctttgcct ccaaccacat
    15781 agcatgtgga aaggtgcatc agcgcggcgt gatccgattc gcgcaagaat tcaatcagat
    15841 gaaggaccgg cgcaacattg cttgccaaag cttcgcgaag gtccggatta aaatcagtca
    15901 aaccggagct gttgacgacc aaatccagat tgcgtgccag ttgcgctcgc gtttcagggg
    15961 caattcccag ccccggttgg ctcacatctc cttcgattac ttcaacgcgc tcggacacga
    16021 actctgcgaa acggttacct agctgttcct gcaacggatc gaaaacggga gactcttcaa
    16081 atattttttc aaagcggcgt tgcgaagttg tcgtccgctg gcggcgaata aggagataga
    16141 tttttccgac cttggggacc tcgctcaaga tctgcgccag ccatactttg ccgataaaac
    16201 ctgtaacgcc aatcagaaga atttttttcc ctgcgagggt ttctcgaaca gaaagtggtt
    16261 cggattgttt tctgccgtta aagacaacca gggcgcggtg atccacttcc tttgggcgat
    16321 cagccggcag aattgccttt cgtaattcct ccggatgatc cgccatgccg agattcttca
    16381 gcaatcgttc atacggaacg cttccgtttg cgctgcgctc aaaggctcga gcaatcgggc
    16441 tgcgaggacg aatcacggga tcaagcaggc ggcccgtggc taccccttta cggaattcga
    16501 gacggtttga aacgaggcgc gatacgccta aatattgagc cagcggacgc atgacatggt
    16561 caagcccctg gctgaccagc acaacattct cagaaccctt ttccgtgagg caggccctca
    16621 agcgctccac gccttccttc ttcaattgct ttttcagcac gtactcgaag tattcctcgc
    16681 ccaacgaatc caggcggtcg cgcgtcactc cagccagcaa agtgtgcagc acacgagtgg
    16741 caaacacacg gtgactcgcg tagagaaatg ggcgaatgag tgcaagagca agcgctcctc
    16801 cgcgacgtgc ccagcgacct gcaaaagtat gggaattcca tgtgaagtag gcaacaggac
    16861 ggacggcacc gagcgcaacc aagctacctt ccacgcgcca aaacgtcagc gcgcgaggat
    16921 ccggttgcgg gggagaggca gggaaactcc cattcgcatg gtggccgtcg ataatatttt
    16981 gggcgctctg attactcaac ggtgagttct gctccaatga tgtaaggaag aatcacccag
    17041 tctggccagt tcgaacctac cattcccagc ccctgaatcg caagagcttg ggagcacttt
    17101 tctacaaatt gaatactcgc cgcgaattgt gaggcagctt aaccatcgaa agtgagtctc
    17161 aagtctgccc ggatccagac ttgatccagg tctgattcta ccctgatcga gctgaactta
    17221 cacagcattg caatcccgag taggcctcaa tttgcgagca aagtcgccct ctccgtcccg
    17281 aagatcacgg gacggagaga ccgctacaaa accgcagtgc atagcagttg tttttgtaga
    17341 ggccgcccgt tttttcgggc gggcagcctt ttttgtcgca gcactgcaaa attcaaattg
    17401 atgcactacc caatcccgga ccctaggatg tggccgaaag ttaactcgga aaccttctgg
    17461 ctttttacaa aggattcaca tgcaattcac gcagcgatga aggtcggata agtaaattgc
    17521 tatccccgca agggtaagta cacattcaga aggttttcaa attttaagga ggattggaat
    17581 ggctagtaag tggaaggctg gcatcctgac tgccgctctc accctgtcga ctatgtcgac
    17641 ggcctacgct ggaccgaaaa acggtcagtg ggacgggaat ggtcatcgcc gcattcaaca
    17701 cgtgctcttg atcagcattg atgggatgca cgtcgttgat tttgcaaatt gcgtgacctc
    17761 cgacacatgt ccgcatctgg ccgaactggg cgagagtggc gtaacctaca cccgcacttc
    17821 gacctcccgg ccgtctgact cgtttcctgg gctgatggcc ctggtaaccg gcggcacgcc
    17881 gagaactgta ggcgcgtttt atgacgtggc ctatgatcgc gttctggcac cgcccgcaac
    17941 cacgaccggg aatggacttg ccgcgggaag ctgcatacag ggcgtgatca atggaacgca
    18001 aacggaatat gaagagggag atgaaatcga tcaaaccaag gtcaatgggg gcggacctta
    18061 cgcatcatcc atcgacggtg gaattctatc catcgatccc actaagctgg tgcgcgatcc
    18121 gttcaacggc tgcaaaccgg tttacccttg gaatttcgtc cgcaccaaca cgatttatgg
    18181 agtgatccac aaggcgggag gctacacggc gtggtctgac aagcatgcgg tttatgccgt
    18241 tgtatccgga ccgaccggga cctcacttcc aagcaatgtc gacgactact attcaccgga
    18301 agttaattcc aaccaagttc ccttagcggg aatcaccacc gcaggcacga actttgattg
    18361 ttctcagatt gccgccagcg ggaatgactg gacgactgac ttccaagcca tccaatgcta
    18421 tgaccagcta aaggtcaacg gtatcgtgaa ccaaatcaaa gggaagaaac acctcggcgt
    18481 cggcgatgca cctgtcccgg caatctttgg gatgaacttc caggcagtca gtgtcgggca
    18541 gaaactgatt gaaaacggtg tcaagggtgg ctatacggac gctgccggcg atcctacccc
    18601 gagcatggcg agcgagatca aattcgtgga tgcgggaatc ggtcagatgg tggaagaact
    18661 ccaggatcgt caccttctgt cttcgacggc tatcatcatc accgccaagc acggccaatc
    18721 gccgatcgat accaaccgat ttttccccat tccaggaaat tccggactga atggcacccc
    18781 gccatccgga atcatcggaa cccttctccc gctatcggcg actaccggac ttggcttggc
    18841 tgaggacgat atctctcagc tgtggctgac gaattcgaac gatacgctca ccgctgtcgg
    18901 aatgctggaa tccaatgcgg cgaaggcggg aatcggtcaa atcctctatg gcacttcgct
    18961 caacaccatc tttaatcctc ccggtgttcc caccactcca ggtccctgct gcttcctccg
    19021 ggaaggtggc gatcctcgca caccggacat tatcgtgttg cccaacgtcg gcgtggtata
    19081 caccggcagc acgaaaaagc aatccgaaca cggcgggttc gcttgggacg acaccaacgt
    19141 gatgttgctg gtatccaatc cgggatacaa ggcccagacc ctccacagct ttgtggaaac
    19201 cactcaggta gctcccacca ttctcaaact tctcgggctt gatccagacg aactcgatgc
    19261 cgtcaggaaa gaaggtacac cagttctccc gggcattgat ttcggtgatg gcgaatagaa
    19321 cagaggaaag ttcagccctg tcgcagggag aaccataggg agtgccttgg attcgggagg
    19381 cactccttgt tttattttca agaggtctcg atcttgggcg tcgcctgatc tgggtctgac
    19441 atgttccgta ggaattcaat gctaggactt gccgcgcacg tccaaaagca ccaaaagacg
    19501 cgcctggcgg attcgattga tctagaggct cgtgggaaag gctcaatccc gctagattcc
    19561 ttggcgagtt cgttacaggc tgtgggccgc gcctgtcatt tcggtttgca ggcgtacacc
    19621 aaattcgacg cagccgacct tagttgtgtt gggctcaact gtgtcaggag tgcgaatgcc
    19681 cttcgtaaat aagcgatcta ccgcagattc gatatattgg gcttcgcgtt gcatattaaa
    19741 actttcgcgc aacatcatcg caccggaaag aatcatgccg actgggttcg caaaatcttt
    19801 tccggccaga cttggtgccg aaccgtgaat cggctcgtat accgaagggc cgggaccgct
    19861 gctcatcgaa ggaatgagtc cgatcgaacc ggcgagcgct gcgccaaggt ccgtcaaaat
    19921 atcgccgaac atatttgaag tgagaatgac gtcgaattgc gcgggtgcaa ggactaactg
    19981 cattgctgcg ttatccacgt aaaaatgttc gagcttcacg tttggatgat tggcggacat
    20041 gcgggtcacg gttctgcgcc acagttgtga agtggtcagg acgttagcct tgtcgacgga
    20101 tgcgagcttt cctttgcgcg attctgctcg agcgaacgcg tactttgcga cgcgttccac
    20161 ttcagcggtc gtgtacacgg cttcgtccgt ggcgcgttcg ccgctcggat cgggttgcac
    20221 atcgtgcttg ccaaaataaa tatctccagc aagctcacgg acaaaatcga tgtgcacgtc
    20281 tttcacgcga tccgcgcgca acggggatag accgtagaga ggcaaccgca gatcgattgg
    20341 gcgcaggttg atgtacaggc cgagcgcctt gcgcagtgca agcaggccag tttctgggcg
    20401 tttcacgagc ggaactgcgt cccacctggg acctcccact gctcccagaa ggattgcgtc
    20461 agccgtcttg catgcggcaa gagtttcggc agggagaggc tcgcccgtcg cgtcaatggc
    20521 gcaaccgcca aaagggaatt cggcaaagtc gaactcgtgg ccgaattccg tggcgcacac
    20581 tttcaagatg cgtacagcaa cgcgcataat ttcggggcct attccgtcgc ccgggagtag
    20641 gacaattttc tttgtttgtt tcgtcatgtt tttagtggca ggcgatgggc gctatttttc
    20701 gatttgtatt tttttacact cggaatggtt tcgcgcaagc ccgaggcgaa atagtatagt
    20761 ccggaacgcc ggacctgtcg aaaaagcggg acaacaattg caaaaattcc cgcttgacag
    20821 cgccggcgaa aatgtatatt actaaaccac atggttaagt ataaccgaga actcgacgcg
    20881 acgtttcagg cgcttgccga tcccacgcga cgcgcgatca tctcggcact cgcgctcgga
    20941 caggcatccg tcacgcagct tgccaggccg caccgcatga gccttcccgc gatcatgaag
    21001 catctggttg ttcttcagaa ggcggggctt gtgacacaac gaaaagtcgg gcgcacgcgg
    21061 cactgccggc tggcggcagc ctcgctaaaa aaagccgaag catggatttc gcaatatcgt
    21121 gcattctggg aaacacagtt tgattcactc gaccgatatt tgtctgaaca gcccaaggag
    21181 gattcgcaat ggccaaagag cagctttccc cggaaacctc gctgacatta cgacgaactt
    21241 ttcttgcgcc gcgcgaaaga gtgtttcgtg cctggacgga cccggttgaa ttgggaagat
    21301 ggtttgctcc ctccgacgaa tatcatgcga ttgttcccga gcatgaattg cgagttggcg
    21361 gaaaatattg cctggagatg catcacaacg gcggaaacat tcaccgcgtg agtggaattt
    21421 acaaagaaat taatcctccc gaaaaaattg cattcacctg gctgtgggat catgaaccga
    21481 aatcggacga gtccctcgtg acggttgaat ttcgcgattt gggtccttcg acggaaatcc
    21541 ttttaactca tgcgcgcttg ccaaacgcgg aacagaccga gaagcacggg catggttgga
    21601 acggatgcct gaacatgctt gggaattatt tgaatatgta gtgtaggttc gaagttcata
    21661 cggattcaaa gttcactgga ttcaattatc acaaacacaa aaaggagaaa tcatggcaat
    21721 cgtcgattcg attttgatgg agatcgagca ggaagcaaag gttacgcaga aagttcttga
    21781 acgcgtccca accgataaat tgacctggaa accgcaccca aggtctctat cgctcggcca
    21841 actggcgatg catatcgccg tggggcaggg ccgtcttgct gagatcattt ccaaagatac
    21901 tcatgaaatc ggcgacatca gccaaccgca gcccggcccc gatgatgata ttctcaaaac
    21961 cttcaaccaa ggcacggcca acgcactggg aacgctcaag aaacttaatg attcgcagct
    22021 gatggccctg tggactctaa cgaaaggcgg gaaggtagtc ctttcggcgc cacgcatcgg
    22081 cgtgatccgc tcgattctga tgaaccattt ctaccatcat cgcggccagc tttccgtgta
    22141 tctccggatg ctggaggttc ccatcccctc aatctatggc cccagcgcgg atgaaaatcc
    22201 gtttgcataa aattgttctt gcatgcattc gcgcggcaag ctttgcgtca attcaaaagt
    22261 ggagcggagc aaggaatcta cttctgctgt agcccggctc ttcagaggcg gggtttaaat
    22321 gacgctacgt ctaaaccccc gcctctaaag aggcgggcta cagcccctag tcagtttcta
    22381 atcaggctga ccaggttgcg cgacttcgat gctaatcaga ttccgcggcg gctgcagttt
    22441 caccggcgcg tttgcgcatc acctgatgcg cgatggcgag cacgtcgcga ttactcacat
    22501 ttttcgacgc atcggccaat gtcacaacgc gcttatagca ttcatcgaga tcttcgcgtg
    22561 tcggctggaa gcccaaatcg agaagcgttg cgcgcagcgc gttgcggccg gaatgttttc
    22621 cgaggaccat ggaccgcttg ggaacgccga ccgcttcagg gggaataatt tcgtaggtca
    22681 gaggattttt caggatgcca tcctgatgga taccggcttc gtgcgcgaac gcgtttgcgc
    22741 ctactacagc cttgttgggg gctacaaccg ctccggtaat ttccgtcagc aggcggctag
    22801 tggcaaataa ctgatccaga tgaacatttg tggtcacacc cagagtggct tgccgtacat
    22861 tcagcagcac gacaacctct tcaagcgcgg catttccggc gcgttcgccg ataccgttaa
    22921 tcgtcagttc gagttgacgc acgcctgcct gcacggcagc cagggtattc gcaacgccca
    22981 ggcccagatc atcgtggcaa tgcgcgctca tcgtgatgcc atcggcgccg tcgagataat
    23041 cgatcacgcg acggaacatg gccgcatatt cttctggcac tgcataccca acagtgtccg
    23101 gcagattgat gattgtggcg ccgccgtgaa cagcccgtcc taccgcctcg caaagaaatc
    23161 cgatttcggt gcggctggca tcttcagcgg aaaattccac ttccgcgcag tgttcgcgcg
    23221 catagcgcac gttgttggaa atcgcttcga ggcattcttc gcgcgtcagc cgcagcttat
    23281 gttgcaaatg cagatcggag gttgcgagaa aagtgtgcag ccgcggatgc gcggctcccg
    23341 aaaggccatc gagcgctgcg tcaatgtcgt cgcgcctcgc gcgcgccagc gcagctaccg
    23401 tgcagccgcg aatttcacgg cacacggcgc gcgtgccttc cagatctccg gccgatgcga
    23461 tagggaagcc actctcgatg atgtccacgc cgagcgattc gagctgatga gccatgcgga
    23521 ccttttccgg aaccgacatg gaaaatcccg gggactgctc accatctctc agcgtcgtgt
    23581 caaatatgcg tacgatctcc atggcactct ccactgcacc cataacacac gcaaagttaa
    23641 agtagatcaa atttataata attgtgactt ctataaggaa taattatatg gctagtttct
    23701 tggacgggct gaaggaagaa ggctggaatc tgtcccactc acggacttcg aaaatcagga
    23761 gttcttaaaa cgttcgtcga tttccacctg gtagccggcg acaaggcgat tcgtttcatt
    23821 ggccattccg acgatggcca tgagttcgtg aaaaatttcg ggggtcatgc ctttcgcctg
    23881 cgcggaggcg gtgtgcgaag cgatgcagta agggcactgg ttggtgacac tgacggcaat
    23941 gtaaatcagt tctttggtca ccgcgtcgag ggcacccggc cccataatct gcttggtgga
    24001 ttcccaggta cgcttgagag ttacgggatc gttcgccaga accttccaga aattagtaac
    24061 gtaatcggtt ttgcgagtgg cgcgaatgtc atcataaacg gcacgcacat cctctcccgc
    24121 atcttcatat tcaatcgggc gctcgattgc catggcgcct ccttgctcaa atagaaataa
    24181 aagaatcggt tgaaaaatta attcaggtcg cgtgagatca tcagcttcag cgtccattga
    24241 tcggctccgt gggtcacgcc tacacccacg ccggtttcga tgttcaccca tttcgtgttg
    24301 tggtccatga cggcccagac ctcgtgaaac tcatcgcgca tggggaggaa tttgcgcaat
    24361 ggaccgtcat cagaatactc ctcgagagct gccgcccatt tgtcgttaaa attataggca
    24421 acgcgagttg ctggaacgaa ttcgagattt ttaaagccgc cggtataatc cgtgtccata
    24481 atagggttgt aaaccagatc caccttaccc aggtgcaatc ccacgatcgg ccgaacttcg
    24541 gaagtgatgt gccgctgctc ccaataggaa taattgacgc taaattcgaa attcactccg
    24601 tagaaaaaca tgtgatcgtg ggcgtgcggg cgcacaaaaa gctcgcgaat tttaaatccg
    24661 ttcagcgatc ccccgcgccc ggttgaatag gccgaataaa cggggaaata aaggccctgt
    24721 tcaaaccagt cgttgacccc gtacgcccat tccaccgcac cattatccga gtggttcgga
    24781 ataattccgc cggggaaatc agcggtttta cgtccggagg gcgtgaaatt attatggatc
    24841 atgatattaa acttgccctg attctcaatc tcggcatcgt aaacctggat ttcgtccgtt
    24901 tgcgcgaaag ttatcgcagg ggccgcgaaa aagccaaacg cgatccacgc caaaacaaaa
    24961 tataaattct tctggaagaa tttcattttt attaatcgtc tcccctaaaa ttcagatcag
    25021 atcttcgagt gagttccgga cctattttcg caataggcgc gggcgatcgc cccgcttcgg
    25081 aatggtctcg ctctttattg ccgcaatgcg atgtcgcagg cgctttgctt gggactccac
    25141 ccctgagccc aaggcccgct aagggtaacc catgtggcag tttacgggca attctccttt
    25201 tcaatccccg tttccatgga gtagtatcgt agtgcgatac aatttgacta tggctcgaac
    25261 acatgcggtt tcgctctgga tgggtcctcg gcaatgcccg ctgtgcggga gcgacaatgt
    25321 tatacgttcg caccgcagcg gcgtactgga atggacaatc ctgctggtgc tgctcatgcg
    25381 gccatttcgc tgccgccagt gcagcgcgag acatctaacg tttttcttcc gagagcggcg
    25441 tggaagtgaa aaggaatcaa ccgtcgatgt ctcgagtccc atcgaggact aattcatatc
    25501 tgccagcgct ccggcctcga tgaaagatca aaaaagtttt aatgagactt ggaaatcgta
    25561 ataccaacta tgcgcggggg tgtgccttgt caaaaacctc gagcaattgg cggatagtgg
    25621 cgtgggtgta acgctgggtg gtggagaggg aagagtggcc tagcaattcc tgaatcgcgc
    25681 gaagatccgc accgtccgcc agcaggtggg tggcgaatgc atgccgcagg gaatgcggat
    25741 gcaaatccca attcacattc gcgagacgcg catatttttt aacgatgcga gcgactgatc
    25801 tttgcgtgag cctggttccg aaatgattaa gaaaaatagc ttgctggtct ccacgccttc
    25861 cagctttcct caatagttcc tggcgcagag gttcgtacgc ttccagtgac agctccgctt
    25921 ttccgccata aggaacgatg cgttccttgt tgcctttgcc gcgcacacga agcataaggt
    25981 cttttcggtc tatatcgacg agattcaatc ccgtcaactc actgacgcgc agccctgagg
    26041 cgtaaagtaa ttcgagaatg gcgcgatcgc gtttcaccat cagatgacta tcgtcgtgca
    26101 tccctctgtt cttgcggcgg ctggaagggc gcttgggcct ttccaccact ccatccagaa
    26161 atgcgttcaa atcttcggcg ctcagaaccg agggaatgcg tttcggcagc ttgggagtgg
    26221 aaaccatgcg cgccggattt cgtacgacga tgccttcgcg gaccgcgtat ttgaagaacg
    26281 atcgaattgc ggcaagcttg cgtgcaatcg aagatttttc caggcgcagg tcgtgcagct
    26341 gggccatgaa ttcgcgaatc atgaggtgcg taacgtcttg tggaggtggc atcgccaggc
    26401 caggtggggt caaaaacagg agaaactgtc cgaggtcaga ttcgtagctg cgtatcgtat
    26461 tcggggaaga atttcgcacc gaacgcaaat agttcaaaaa ctgctttact gcggaatcca
    26521 tttcccccct gtaaaactgc cgacctggcg gatttccgct ggacgatgta caagcgctta
    26581 ctactgagca gttcccacag gttcaggaac agccgcggcg ggagcttccg aaacaaccgc
    26641 ttcccaatcg caaccttcct taacgcaact tctaatagcg ccaaatttac cgcgcttttc
    26701 aacaataaat gccgcgccgc atttcgggca aggctcggca atcggcagat gcggcgttgt
    26761 aaaatcgcac tcgggatatt tattgcaacc gtagaaagtg cgtctgcggc ccttcttcgc
    26821 aacgcgctcc accagttcgc ccgtctcgca cttcgcgcac ttgattccta gagtcttcga
    26881 ccgaataaat ttgcattttg gatacgccgt gcagcccaca aattcgccat attggccgta
    26941 tttcttgacg agttgtgaat tgcattgcgg acatttttcg tccagcagct cgtctggctg
    27001 cagggctttg cgcgtgcctg ctttcaggcg gcgcgttgtc ttgcagtcgg gatatcccgt
    27061 gcaagccaaa aacattcccc agcgtccgcg cttgatgatc atttcgcggc cgcaattgtc
    27121 gcaggcttca attttcgccg cgccatcttc accctcggga ggcaactccg gcgagagatc
    27181 gcgaatgaaa tcgcaatccg ggtaacgtga acagcccaga aagaatccgt ggcggctgat
    27241 gcgttcgaga agctcgcctt cgccgcactt ttcgcacttc tgtcccgtag gaatcccagc
    27301 tttgtaggat tccatttcgt ccttggcttg ttcgaggtcc gcatcgaacc ggccatagaa
    27361 ctccttgacc gctcttttcc agggaagttt tccttcttca atttcgtcga gttcctcttc
    27421 catgcgagcc gtgaagccca catcgaaaat atcctcgaag ctttttacga gcagaatgct
    27481 tactttttcg cccagcatgg tcggagcgaa gcggccttga tccttggtca cgtattcgcg
    27541 ttcgacaatc gtcgaaatga tggacgcata agtcgacggg cggccgattc ctttttcttc
    27601 cagctctttc accagcgtgg cttccgtgta gcgcggcggc ggctccgtaa aatgctgatc
    27661 gggccgcaaa ttttccagct ttaagatttc gccttcggtg agcggcggca atagcgcctt
    27721 tgcaggatct gcttcaccct cttcgtcgtc atccgcggat acctggtaga cagccagata
    27781 accatcaaac ttctgcaccg aaccgctggc gcgaaacgtg taatccgcgg cgcttacgtc
    27841 aaccgacgtt tgatcgaaca cagcaggaac catctgcgaa gccacaaagc gctgccagat
    27901 tagctggtag agcttgaaca tgtcttcgct caggaacggt ttcacgtcct cgggagtgcg
    27961 cgcggcatct gtcggacgaa tggcttcgtg ggcgtcctgc gcatctttct tggatttgaa
    28021 aaaattcggt ttttcgggca ggtaaggttg cccaaacttt gcgccaatca ggcctcggac
    28081 ctggtcaagc gcgtcgttcg atacgcgcac cgaatccgta cgcatataag tgatcagtgc
    28141 aacggaccct tcttcgccca gttccacgcc ctcataaagc cgctgtgcca ggcccatcgt
    28201 gcgctttgcg gtgtagcgca ggcggttgta ggcggcctgt tgcagtttgg aagtggtgaa
    28261 cggaggcggc gcaaaacggc gcttctcttt ttgagtgaca ttcgcaactt tccactccac
    28321 gcttccagtc gcagcaacaa tcgcatccgc ctcagcttgc gtgtggactt caatatcttc
    28381 gcccttaaac ttggtaagct tggcctcaaa cgaaggcggc acgccggcat ccaacatcgc
    28441 gtggatcgtc caatactcca cggggatgaa cgcacgaatt tccatttccc gatccacaat
    28501 caggcgcagc gcaaccgttt gtacgcgtcc agcggataaa ccgcggcgca ccttgtccca
    28561 taaaagtgga gaaattttat agccgacgag gcggtccagc acgcggcgcg cctgctgcgc
    28621 gtccaccaga ttttcgttga tctcgccggg atgctcgaag gcagctctaa ttgccttgga
    28681 cgtaatttca ttaaacatga cgcggtagat attcttgcgg gcaatcacag gcttgccctt
    28741 ggcgatcacc gtcttcacct gcgtcttggt tttcttcttg ccctttttcc ctttgccctt
    28801 gtctttttct ttcgctttgg cttcttccgt ggcgatttct tcgtcggtaa ggaaaatcag
    28861 tttaggaccg ctcagtacgt cggccagatg cgcgcaaatc gcctcacctt cgcggtccgg
    28921 gtcgcccgcc agatacaccg catccgcagt ggacgcagct ttttgtaact gcgtgactac
    28981 attcttttta tccggattta cctcatacgt cggctcaaag ccgtggtcaa tatctatgcc
    29041 cataactttc ttgggcaggt ccatcacatg cccgtacgag gctttgacca cataatttcg
    29101 ccctaaatat ttattgattg tcttggcttt tgcgggcgat tcgacgatga ccagcgatct
    29161 tcccatgact tttttcgaaa cctcagtgct tcctgaagga agctttttcc ctatcgatat
    29221 cttagcgtgg cccccgttga cactgtttgt caacgctaga tagatgcgcc atgtgtcctg
    29281 tttgatgcta agcagtggcg aaaagccgaa tagatccttt cttttgaata aattagaaag
    29341 ctggctctga acagtatgat tgcgtgttgg ttacaatgca ggaataccgt tacagcagga
    29401 ttttcagaaa ttgcttccct ggcatttgcc tgaccattct cttcatttcc atttcgcata
    29461 gtgaggccag gacttccgac gagttcaatt ccgtcttttc tacgagttca tccacatgaa
    29521 tggattcgtc cgtatccagc agggcaaaga tttttttctc cgctgcggat aacgccttct
    29581 caaataagga tgcgcgctcg gccttagagg tcgcttccac aggaaacagc tcaacccgaa
    29641 tttccgtggg caactcctcg accacgtctt cccaagaggt gaccagcttc gccccttgct
    29701 tgatcatctg gttgggggca aaacttacgt ccaccgttac gtttcctggg actccataca
    29761 cttcacggcc gaactccatc cccagtcgcg cggtgatcag agatccggaa tactgcgctc
    29821 cctgcaccac caatacgccc agggccattc ctgcgaccac acgattccgt atcggaaaat
    29881 tttcaggagc cggatgcgtg cccaaaggga attcgctgat cagcgccccg cgcttttcca
    29941 cctcggcgaa caattttttg ttttcttttg ggtagattac gtccactccg gtgccgagca
    30001 cgccgatcgt ggctccgttt gtggctcgac aagcgccttg atgcgcaagg ctatctatgc
    30061 cgcgcgccat cccgcttacg attacgaggc cacgctctgc caaatcatgt gccagacgct
    30121 cggcgatctg gttgccgtag ggagttgggc gacgcgtgcc aaccatggag acggaatgcc
    30181 gattgagcag gctgatatct ccccgcacat aaagtagagg aggcgggtcg tagatttcca
    30241 gcagtctttg cggatattcc ggttcgctcc agttcagcag gcggcagccg agggtgcgca
    30301 cctgcgcgag ttctgcttcg gcatctttat gcgcgagttt cgtgtggatg gcccgagcgg
    30361 gcgcgactgg aaggtggcag gcttcaagtt cggtaaggga agcgcgaaaa acgcccgagg
    30421 gagtgccgaa ttggcgcagc aattttcctg tcaggcgcga gccaaggccc ggcgtcatcg
    30481 aaagtgacag ccaatcgagg gaagcttcca gttccacgca ggcgcgaccg tactaccgtg
    30541 tcactatccg tgtcaatagg tggtttgcgt ggcataggct tcagcctgtg gggttaagcc
    30601 tttacaaggc ctgagttcca caggctgaag ggtctgtgta agaactgcaa aattgaagct
    30661 tcagcggcta aataggttgc ggaaaaagtc gtttattctg tcattccgag cgaagcgagg
    30721 aatctctctt cgatttaagt gtaagaaaat aaagaaggag agattcctcg cgtcgctcgg
    30781 aatgacaaaa tgttgagttt ttccgcagtc tataaagccg cactcatttt ccaggacttg
    30841 tgtcgtggta aaagccacga cccacaaaga attctgcaac tctcactcgg atcctgatgc
    30901 ctatgttatg caagtcttct tgccagccat tacgtatttt gataaggatg tggcactgcg
    30961 aggagaaggt aatgatcaac ttgcaaatga gcgcgcgcgc gggattgatt tctgtgtgtc
    31021 tggtcatggc cgcatggaag cctgcggcga gccagactgc cgcttcgatg aatcgagcga
    31081 aaacagaaaa aatctccgtg agccagaacg atatcggcgg cgcggtcagc ggactgaacg
    31141 gcccggaagc gggagtatgg gtgatcgcgg aaacaacgga cctgccaacg aaattccgta
    31201 aaattgtggt gaccgatgaa cgcggaagat ttgttatccc cgatctgccc aaagggaatt
    31261 acaaaatttg ggtgaggggc tatggcctgg tggattcgac acccatcgtt tcagcgccag
    31321 ggaaaacgct ttcgctccaa gcggtgcttg cacccgatgc acgtgctgcg gcacaaattt
    31381 atcccgcaaa ttattggtat tcacttttgc aggtcccctc ggcgagagag tttccatttt
    31441 tgaagccggc acgcaattcc gcttcgcagc gcgggcgccg cgaaaacgga caggccagcg
    31501 tgttgtccgg cgggattcac aatcaggcgg aatggctgtt tggcatgcga acgtgcacac
    31561 aatgccacca gctcggcgac aagtttacgc gcgagatcga accgagtctc ggcactttcg
    31621 attccagcgc cgcagcctgg gaccggcgcc tgaagtccgg ccagcgcggc ccgctgatgt
    31681 ccacggcctt aaaccagctt ccgcatgatc gcgcgctgac catgttttcc ggttggagcg
    31741 atcaggtagc ggccggcgcg cttccgcccg tgcctccgcg tccgcaaggc attgagcgaa
    31801 atgtggtgat cacggaatgg gagtggggac gcggtcccgc cgattgggtg cacgacgaaa
    31861 tgaccacgga caaacgcaat cccaccgtga atccgcacgg acttgtctat gcaacttccg
    31921 aaggtgccgg aacgattgaa gtcgaaaacc cactgacgca tgaatcgagc tacatcgata
    31981 tccccacgcg cgtggaccag aaagaaatcg cgccttacct catgtcgctc gatgttggtt
    32041 ttgcttctcc ctattttggc gagcagcagc tgtggacggc taccagcagc cctcacaatc
    32101 taatgatgga tgaaaaaggg agagtgtggg tgacgtcggc gattcgcggc aagaacgatc
    32161 ccccctactg caaggaaggt tcaaacaata tttttgcgaa gaacgatccg atcgacgtga
    32221 gtatccggca actttccatg tacgatccgg aaacaaaaaa gtggatcatg atcgatacct
    32281 gcttcggcac gcaccacttg caattcgcgc aggacaaaga caacacgttg ttctttagcg
    32341 gcgaagaggg cgcggtcggc tgggtaaaca cgcggctgta cgaagagacg cacgacgagc
    32401 aggcggcgca gggttggtgc cctgcttata tcgacacgcg aggtgacgga aaattcgatt
    32461 ggaaaacgga caatgctgtt tcgggatttc catacggtct cgcagtaaat cctgtggacg
    32521 gaagtgcgtg gtatgccgct gtcggcgtgc cgggaaaact tgtccgtgta gatcgcggca
    32581 caaacccgcc ggccacctgt cacacggaag tgtacgagcc gccatacgag agttcgagca
    32641 tcgccggcaa ccaggcgttt ttcccgcatg gtgtggatat cgacagcaac ggaattgttt
    32701 ggacttctct agcgggaagc gggcacctcg ccagcttcga ccgccgcaaa tgcgctgtgc
    32761 ttagcggacc gaatgccacg gggcagcatt gccctgaggg ttggacgatg tatcggttgc
    32821 ctggacctct cctgaaaggt accgacgttg gcagtgcgga ttatccttat tataactggg
    32881 tcgaccggtt cgacacgttc ggcctgggcg agaacattcc gatcgcgacg ggcacaaact
    32941 cagattcact tctcgtatta caacctgata ctaaaaagtg ggtgatacta cgagtgccgt
    33001 atccgctggg attttattca cgcgggcttg atggccgcat cgatgatccc aacggcggtt
    33061 ggaagggacg agggctgtgg tctaattttt cagaaattcc ggtgtggcac atcgagggag
    33121 ggaagtcggc ggtgtcaatc ctgcttcatt tccagttgcg gcctgatccc ttggcgcatt
    33181 aaatttgggc agacatcaga tgagctacct cattgttgaa aagtatttaa tgcgatattt
    33241 gcggggatgc cgctcatttg attggcgaga atcaaaggca aggaagaaag ttttctcttt
    33301 agacagatga acgcaaatac gaacaaaaaa ttaaatgtca ttacttctat cggactggcg
    33361 ctcggaggag cattcggaat ggccggaact tttgtcggcc aggcgaattt cagaaatatt
    33421 tcttgggcaa tcgatgggct tggcttaatt gtagctacca gcctcctcac aattaaatat
    33481 ttcagaaaag ggtgtgattt tattgcagct ggatttctgg tgttcgcgat tggcgaatcc
    33541 gtgatgcttg cgggaacggc gtcggggctg gccgcaagtg cgccttcttt cgcagccgga
    33601 acttcaatgt gggcaacggc gatattggtc gtcagcattc cagcggaatt ttcattatgg
    33661 gtccggctga tcggttttat tgcttccgcg ctcttctatg ttacggctac aaaaattttc
    33721 tggggcgcgc ccatcactcc actttcgtct ccattgccgt tttttgccta tccgtttcta
    33781 gtgcttacat tcgttggatg gatcgtgatg ttgctaacgg aaaataatgg cggtaacgag
    33841 gcctagaaaa ctccagcagg acaatctgcg gaagaaaata aacttcttgt cattccgagc
    33901 gaagcgagga atctctcttc gatttatcta cgagagaaaa cggagagagg gtcctcgctt
    33961 cgcccggaat gacagaataa attgctcttc cgcggcgcgc aaagtttgtc ccacttcaag
    34021 ctgcaaatta gattaggcga gtccctcaat gattcggatg tcgcgctcgg cggcaccatc
    34081 aaggacgcgg cacgccgcct ggtaagcagg gctttcaaac gtggtcgttg cctgctgcac
    34141 gctttcgaat tcgatcacca cggtacgcat gctcttcccc gcttcgtagg ttttcgaagg
    34201 caggccacgt cccagaaaac gtccgccgcc gccctggatt gcaggccctg cgagcttcgc
    34261 atattcagct actttgtccg catccgagat cgaacgatag aaacaaatcc aatatccttt
    34321 tgccatgggg tgctcctaag gaaaattttc atcgtattgc cgcggaatcc gcgagtggca
    34381 atgcgaagca ctaccttgca cattgaccga cattgtttct tagatttgga agtctagctt
    34441 ttctgatcaa acgccgcaag aagactcagt caacctttcc ggcttgcaac tgctgtggga
    34501 atttatcttc tcagggaaac accaagcaag atttggctgg ccacgggctg gtcagcattg
    34561 aacgcaggaa taaatttcca gcctgtggtt gatcgcaata gttgctcatc cactttttca
    34621 acattcccaa taacttccac cgaccgaact ttgcctgcgg aatcgatcac cagttttaat
    34681 gtggattcgt ttttagcaag ttcttgctcc atcgcggtga gcggaggttc ccgttccatt
    34741 aatatggctc tcggcggtgt aatgggtgga gcaactttcg cgttcttagg cgtgagtcca
    34801 ggaggcgcag aaacgacgac cgttccatca aaaatcttag cgtagctttg cctgccggga
    34861 tgggtcacgg tcagtactcc gtccacttga gagcgaattc ctcgcgcgag cgctcctgaa
    34921 acagcgctcc gaatgcgatc gatatccgaa gctggccaca tttcctgtag ggcatgccag
    34981 gcatccggct cacggccgct ataaagataa gaccaaacga tttccagaac ttttattttt
    35041 gcgctacgca tgcggcttag ttttacgaca ggaatcatgg aggcgggatc aagttttccg
    35101 tcacttttct taaaatcgtc aaggtcctcg ggaacgatct tggcccgttc ttcggcaatt
    35161 ttttgattat aatatgggcg gaactcggaa cttacatcca gcaatctgcc gcgagcgaag
    35221 cgaaggacga tgggcggcgc gaagtccaag tctgataaac gtaaattttc gaagccttcg
    35281 accgctgctg catcgtcggt ccatatttcg actcgcccgt ccagatcggt atctgcggca
    35341 ctgaaatatt cgccgccggc gattctgcgc agcagcattg ggggctgttt caacgaataa
    35401 atctcataag ccatgcagca ctcggtcttt aattttttta cctgcagcgc gagaacggga
    35461 gcacccacgc ccaggtccac tccaagcgca tcgatatcca gttcggaagc cgcggaagtc
    35521 agggcaagtg tttgatcgct ccagctgagg gatgcatcgc agacgcgtgc cgctaattcc
    35581 tgatttctgc cggctcccac gtgcacttta acgcccgtca ggaattgcac gtcgaagtcg
    35641 ccaacaccat cgcggcatag gacatcccgg ttttgcgcat gacaggcact tgctgtaagg
    35701 caggacaaca aaaatatcac cagaccaaat ttcagccgcg gccggaataa tttaaacgcg
    35761 agtccgatag attttgccaa tgtgaagaca ggccacaccc atgggaggat ggtgtgtcgt
    35821 gccgctacag gtgcgctagg aacgaaagac ttcatgacca gccatgcttt caaatgcggc
    35881 tccaataaaa gcgagggaag ccgcgacaca ggctcggccc gtttagagtt tcagaagttt
    35941 gacgagcgtg ccgccaagaa tggcttcttt ttgagcgtcg ctgaggaacg gtgcattcaa
    36001 taccaaatcc acattggaat gccagttgaa aggcatgtcc gttccaaaga cgacttggcc
    36061 tactccctct tctgcaacaa ggtggcgcaa tccctcgtct gaaataatga tcgtgtccac
    36121 aaggacctgc gttttcaaat attcgctggg atgcttcttg ttcaggcaat cggcgttggg
    36181 cctcacttcg caggcgattt ccgttcgccc gagataggac ggcaagtatc cgcctgaatg
    36241 cgccgcgcag atttttagat tggggaaacg atcgaacgca ccattcacaa tcatccgcgt
    36301 gagaaaaatc gtggtctcca ggggattgcc aataatgttg cccagatcgc ctcgaccttt
    36361 ccacgcatct tgcttgatca cattttcggc gccctgcgga tgcatgaaaa ccatcacgcc
    36421 cagttcctgc gccttggccc agaaaggatc gtacttgggc aacgaaatgt cttcgccctg
    36481 aacgtgaccg ccaatcgccg cgccgcgcgc gccaagtttc ttgaccgcgt actcgagctg
    36541 ctcggcagca agatcaggaa attgcagcgc gacggaggag acgccaacgt agcggtcaga
    36601 atgagcgttc acccaatccg ccagtttttc atcgtgcagc ttaacgattt ttgccgcgag
    36661 atcgcgatcc gcgccgtaga accaaaaacc gttgatgctc aaaacctgga cgtcgattcc
    36721 cagcccatca atcgcgcgaa tgcggtccag tcccatcaag tttgagccac gcgcgcgcgg
    36781 tccggcattt ggcgcgagtg gggtgtcttt cacgatgtcc cagacttcag ggatgacgca
    36841 gtgagcatgc acatcgacca ccttgatgcg cttgccgcca atggaaactt cgcggcgttt
    36901 tacgggagcg gctgtcgcgg ccgcgccctg cagcgcagca tgggcaaatg tgttccccgt
    36961 cagcaaggcc ccggtcgttg cgcccgcgac ggtcttcagg aaatctctgc gatttttcat
    37021 gcttcgctcc attccatttc cccggaattt ggacggcaca aattgactcc gcaaatgggg
    37081 atcattgaat attgaatttg atcttcatcc taatccgatc gaagtgccgc tagtaaacct
    37141 cttgccgtgg cggaagtcaa gaagccgtcg gcgcatgtgt gggctatgct atattcaatc
    37201 gttccgtggc gttagagaag atatgggcaa actgcgcatc tccattgtgg aattcctgaa
    37261 taccgcgccg ctcgtctggg gtttcacgga cggacctctg acgggcaagt acgatctttc
    37321 gtttgccgtg ccttcgcagt gcgcggacga tttgcgcgcc gggcgcgccg atgtgggcat
    37381 cattccgtcc attgagtacc agcgcatgga ggatgttgtc gcgcttcctg gaatggcgat
    37441 cgcctccaag caagaagtgc gcagtcttct cgccgtttcc aaagtaccca tcgaaatggc
    37501 cagaagtttt gcgctggata cgaattcgcg tagcacagta gggctcacac gcattctcag
    37561 ccgccgccac tggaacatcg atcccgaatt tatcgacatg ccacctgatc cggacaaaat
    37621 gctggcgcgc gcggatgcgg cgcttgtgat cggcgatccc gcgttgcgat tgcggctgga
    37681 aattgatgcg ctgcaggcca aaactccatc gggcgaagat tgctgttgct gcgacggaga
    37741 agacgaacat cccgtgaagg gcattgaaac tttgtttgtg tatgacatgg cccagcaatg
    37801 gcgtgagatg accggcttgc ctgccgtgtt ggcgatctgg gtggcgcgcc gtggcgtgat
    37861 cactccggaa atggctgtgg acttccaggc ttcacgcgag tatggacttt cgcacattgg
    37921 cgatatcgct gaaggcgccg cgctaaagct cgaacttccg ccgcgcgaac tggaacttta
    37981 cctcaccgaa aacatagatt tttctctgga cgacgagaat ctggcgggcc tgcggcgtta
    38041 ttatgatgag tgcgcgctgg ccgggctgat tccaaaggcg cgtgatctgg aatttgtagg
    38101 cggaatgctt gctgcggaaa aatgattggc ggaccgattt tctttttggt tttgtagccg
    38161 ggtttttgta gctcgcctct tcagaggcga ggatttagag ttcacctcgg caatcatttt
    38221 tggtgccggt caggctctta aaccggtgcg tcatagtggc gcggctacag taaagcagcc
    38281 tgcaacgcag ccaattgatc tcactattga atcagcgacg cggcgaaatc gtgcggaatt
    38341 gaggatggaa tcgcaatggg actgacacag caagaagctc tcgacctgtt tcgctccgat
    38401 gatcttgtcg gcatcggcat ggctgcgatg gaagtacgcc gcaagaaaac cgatccgcgc
    38461 gtcgtgacct atcagattga ccgcaacatt aattacacaa atttctgcac cgagtattgc
    38521 tcgttctgcg cgttctaccg gccgttggga tcgaaggaag gctacctgct ttcgttcgag
    38581 acaatttaca aaaagatcga ggaaatgatt gaattgggcg gcacgggaat tttgttgcag
    38641 ggcggcctgc acccggattt gcgcgtcgag tactacgaaa atttattgcg ttcgctcaag
    38701 gagcgctttc cgcaagttca tctccactgc ttttccgcgc cggagattgg ctgcatcgcg
    38761 gaggtgtcgg ggctgagcgt gcgcgaagcc attacgcgtc tgatggccgc ggggctcgat
    38821 tcgattcccg gaggcggcgc ggaaattctg gacgacgaaa ttcgcgcgcg catttcgcgc
    38881 ttgaagtgca catcggacga atgggaagac atgcaccgcg tggcgcactc actcggcatg
    38941 cgcaccaccg ccaccatgat gtttgggtgc ggcgaagagt ttcgccatcg cgtaaatcat
    39001 ctggagcgct tgcgccgcat tcaggaagat acgggtggat tcaccgcgtt catcccctgg
    39061 atgtttgcac cggatcatac gccgctcgga aaaaaagttc cggaagctac cgcggtcgat
    39121 tacctgaaga cgctggccat cagccgcatg tatctcgaca acatcgatca cattcaatcg
    39181 agctggctca cgccgggaat taaagtttgc caggtaggtt tgcaattcgg cgcggacgat
    39241 gtgggcagca tcttgatcga agaaaacgtg gtcttcgccg cgggagttcg caaccgcacg
    39301 aacgaaggcg agttgcgccg catcatcgaa gatgcaggct acaggcctta tcaacgcgat
    39361 acgctgtacc gctcctattc gctgaaataa atgttgctgc gggttcgtag cgccggagtc
    39421 tcgccggcag atttccgtgg ctatatggac gcgcaaaacc tgccggcgag acgccggcgg
    39481 tacgaaaccc gctgcggcgg ttccaaaatc taacatttca actggattac taggatcagg
    39541 caagcgcttc ggtgcgtttg aatactagga tcttggtggt catgatttgc acgggttcac
    39601 tgttttgatt gctggtaatg acgcgtactt tcacgatgcc ctgattgggc ttggaccggg
    39661 agggggtaat ttccagaatt tcgctatttg cccgcaacgt atcgccgcgg cgggtgggcc
    39721 tgggccactc aatttcggcg cccacaccga tcagccctgt ggcgaaaggc aggccgcccg
    39781 tcaccataag atgcatagtc gctgccgccg tatgccaacc gctcgcgcta agtccacgaa
    39841 agacgctttg ctgcgcaact gcctcgtcca aatggaaggg ctgtggatca aattccgcag
    39901 caaacgattt gatacgctct tcatccattt ggtaagcgcc cgaggtaaat ttctggccca
    39961 catgaagatc ttccagatac aaagccttgg ctgcgggcgc ggacattgcc aggggcggcg
    40021 cgattttagc cgcagcgggg ttggacgtgg ccgctgttcc agaaccgtag gccggtttta
    40081 cgccattggt gctgtcgaga gctgcattcg ccatttcgtc cgcgtcgcgg ttttcttcgc
    40141 gcggcacata ttgaatggtg aagctttcaa ggcctgcggc ttgtttcttc gcgcgttcgt
    40201 gcagcgggcg caaatccgga tgcttcacct tgtagatgcc cttaatttga ttcacgatta
    40261 attgcgaatc gctgtacacg cgcaggcggc ggatgccgct cgcggccgcg tagtcgagcg
    40321 ccgcgatcaa cgcgtaatat tcggcgacat tgttcgtggt gcgcccaata tattttccga
    40381 gggactcatg cttttttccg tccggacgtt tgatgattac gccataagct gcagggccgg
    40441 gatttccccg cgctgcgccg tcaatattcg cggagtaggc gccttcctgg cgcgtggatg
    40501 gaatttccgg ctcgtcgaat aaatgtcctg atgatttgtg cgtgggtttg atcatcgact
    40561 caaaacgcca tgactatttt tcatgggccg gggaatttgg cgcgtctcca gcggaagctg
    40621 tcgcggatgc ggaattagcc gagagttcgg gagacgctgc gtagtacaga atgcgcgtgc
    40681 aagtctcgca gtgaaaaaac gcatcgttgt tttcgcgttg cagttgctga atcacgtgag
    40741 gccggatgtg gacgccgcac tggccgcaac cttcgccgcg aatttcggcg agcgcgattc
    40801 cgccgtgacg tttggcaatt cgctcatagt gatcgaccaa atcctcaggc acggctgcca
    40861 tgtccgccgc gcgcacagct tcagcggcgc ctaattcttt ctgcgtggcg ttatactcgg
    40921 cctgaatcgc ctgccgctcc ttggcagcgc tggcttccac ttcctttacg cggatttcag
    40981 cggccttcac ttcgcggtcg tattgctcgc cggaaaccat gcgttcgagc aggcggtcct
    41041 ccgctttggc aatttcgtct tccgcgttct ggatttcatg caagagggct ttatacgctt
    41101 cgttactttt cacttctccc gtttgattgc ggtatttctt tgctcgttct ttccactgct
    41161 cgacgtccat ttcaaatgtt ttgcgttctt tttgcccggt cagcagggcc tccttggcca
    41221 ccgcgacttc gtgcttcgct gccgcaacgc gcgcctccgc ttccgccagg cgcttgggaa
    41281 actgcgccag ccgctcgcga attccgatca ggcgcaggtc aacggcttgc agttcgatca
    41341 gaggcttgag atgaggatgc atggaggcag cattttagca cacctatcgc ggatggcccg
    41401 gatttcaaat taataggacg cgcagttttc cccggcgata tttcgcgtta gactcgttgt
    41461 tcattttgta gcgggctccg gacggggctt gggctggaat tctcaggagg ataccgaaat
    41521 gccggatgta catattttgt ggcttgcggg acgcaccgtg gatcaaaagc gcaaagtggt
    41581 cgagggcatc acgaaggttt tagccgaaga cgcgggggcc aaacccgagt cgactcatat
    41641 cgtattcgtg gatattccac acacggattt tgcgtcgaat ggaatgttgt acgcggataa
    41701 aaataaaaaa gcgtagtcga gttatgtggc ataggcttta gcctgtgggg tttggtgcgt
    41761 gcaaggacca aaatccatag gctgaagcct atgccacgcg gggctagtcg atctggtgga
    41821 cttccatgga ttgatcgatg gtaaatgtcg gaaagtgtgg cggcgtcacg cccgttggat
    41881 tgctcacggg cggcgccgtg taagtggcgg taactgtaaa agaatctccc gatgcttggg
    41941 cggaatacgc gtagccatcg cgattggtgc gggtgatgtt catcgttcca gaggacgaca
    42001 gcgtctccag atcagtgtat gacccattct gcgtgaaata taatcgttcc gcctgcgcga
    42061 tggacgtcag gtccatcttc acgcctgtca aactgatatt ttgcgtcacg gtcattcccg
    42121 gagcaggagc agcttgcttt aaagtgtatg aataaattcc catcccaaca gctgctgtta
    42181 tcagcaggcc gagaactcca aatattccct tcatggcaag tttgtcctca atgaaaatta
    42241 tacgccgctg cgaaattcgt gggtgaacga gcacgctttg gtgtgtattt cccggcaata
    42301 cccgccttaa cttgagattt gatcgtgtta tcgactatag tgccaatgtt tgacggcgaa
    42361 cctggaagga tctttttgaa aatttgtcta agggagcgct atgaaaagaa aatcgcgggc
    42421 ttttggaagt caccgcaaaa tagttcccca cgcgtcccac agtctgcagc aatccataaa
    42481 agtttcttgc ctgttttttc tagccgctgt gttcttgcca gcaatattcc ccggaccgtc
    42541 catccatgcg gcgtctcggg atacggctgt ttccgtgttt gccaccagta ttgtgggaaa
    42601 acaagtggag ctgaacggtt cgccgatgtc cgcgggcgcc acactttttc ggggcgatgt
    42661 ggtgcgcctc ggtgagaatt ctacggctgc gctgcaattt ggaaacagtt cggtgcttgc
    42721 ggcgccgctg acggagttgg tcgtcgaagc tcaaagtttg aaattacgca atgggcggct
    42781 tcaagttcga gtgaacggaa aagagccatt cactgtttcc ggatcttttt ttcacttgaa
    42841 tattgcaggt gcggaaggca ttgctggttc agccgagatt cggttgggcg gcgcgcatgc
    42901 ggaagtttcc gccattgcgg gcataacgga actcacgccc gctggcgatg taccgtacaa
    42961 actgcaaccg ggcgaaacgg ccacgtttga cgaaagcgcg ggacaacctg ccgcaggcca
    43021 aagcacaact aatcccgcgg ctggacaagt gtctcgtctc attccacaag tgcagatcga
    43081 tcgcgcatcg cagcacattg ttgcggaagt ttccagcggc gttttttgga atgacgattt
    43141 gcgcagcggc ccaaccgggc gggcgcacat tacgttgaaa gacggatcgc aactgaatct
    43201 ggggtccgat tccaatttgc gcatcgtcca gcacgacgcg caggcgcaac agacttctct
    43261 cgaccttctg atggggcgcg tgcgcggaag aatcacaaaa ttgtccaagc cggatgcgaa
    43321 atttgaaatt cacacgccgg tgggcgttgc cggactcgtg ggcacggatt tgtcgctgct
    43381 agttacaaac gatttcacgg agctgatggt atttgaaggc acggtgcgtt ttacaacctt
    43441 gaatggtcag tccgtaaccg taacggccgg gaacaaaatt cgcatttcca aagccggagc
    43501 cctcgatggt ccgcttccgt tcgcggctcc ggaagcccag accgcgcaga accttaccga
    43561 tatcaccggc ggagcaaatt ccgccccagt catggctgcg gcgcggccga cagtccccat
    43621 gatcattacg ctgacgggcg ctgcggcggg cgtaggcatc ggagtttggc agggcactcg
    43681 cccggctgtc agtatctcgg tcccctgatt catcgaatgt atttaataaa tgaaaattta
    43741 cagttcgtat tctgcatgtg tctcgccaat cccctttttg aatctatctc caccacggct
    43801 tgacccatct tcggattggc tgttaccgtc gtaaagaaga ctcatgatgg agcgaattcc
    43861 ccagagacga ataccagaac tcgacggtct ccgcgggatg gcgatccttt tggtcatggt
    43921 gggccattat ttttctgttc cgggagttgg cccggtcagt cttctgaacg gatattggtt
    43981 ccgcctgggt tggacagggg ttgatctgtt cttt
//
LOCUS       DASZMB010000232         3364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2138803, whole genome shotgun sequence.
ACCESSION   DASZMB010000232 DASZMB010000000
VERSION     DASZMB010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2138803"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1902)
                     /gene="glgB"
                     /locus_tag="VG272_10975"
     CDS             complement(<1..1902)
                     /gene="glgB"
                     /locus_tag="VG272_10975"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HWF14249.1"
                     /translation="MSTVNPSPEELPADLEPVSEFDRDLGLLLRAENRDPFRLLGPHI
                     VEETVEEHENRRLVVRGFFPHAAYASVLPRGASDPIPAARVHADGLFEAVLPLAPELP
                     IPPSSYVWKLEEVGRPAREIYDAYAFPPLLSDFDLYLMGEGTHYQKYEKMGAHPEVVD
                     GVAGVQFSVWAPNAMRVSVVGDFNQWDGRISPMRNRGPSGVWELFVPELAEGATYKYE
                     IRPMSGGAPLLKSDPYGFRAELRPNTGSIVTRLDHHKWNDAGWMDWRARNDWLSSPIA
                     VYEIHLGSWRRNAQDPWRWLSYSELADQLIPYLKQMNYTHVELMPVMEHPFDGSWGYQ
                     TLGYFAATSRYGTPADFMAFVDRFHQAGIGVLLDWTPAHFPSDGHGLGEFDGTHLYEH
                     ADPRQGRHPDWGTLVFNYGRNEVQNYLISNALFWIDKYHIDGLRVDAVASMLYLDYSR
                     KEGEWVPNEFGGRENLAAIAFIKRLNEVIYARHPGVLTIAEESTSWPMVSRPTYLGGL
                     GFSLKWNMGWMNDTLSYFAVDPIYRKYHHSRMTFSMLYAFTENFVLPISHDEVVHGKG
                     SLINKMPGDLWQQFANMRLFFAYMIAHPGKKLMFMGSEFGQRSEWNHDAPLEWNLLEW
                     DTHRGLQTLS"
     gene            2247..2639
                     /locus_tag="VG272_10980"
     CDS             2247..2639
                     /locus_tag="VG272_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14250.1"
                     /translation="MTTSFPTMLSLARIIPIGGLVLAFTCSPARAQSSLSGTYNCVSV
                     EVNGTAHPCKAPSIEMNSDGSYQILAERGTYEILKGHWLALSTSKNHGRARLDGSRTI
                     IFEFESSGKKSKITYRRKYQRPPAWVSS"
     gene            2960..3181
                     /locus_tag="VG272_10985"
     CDS             2960..3181
                     /locus_tag="VG272_10985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14251.1"
                     /translation="MSIFEQGDVEEVPAQRLWRAVIATTVQEWVSGPLRMKREAEKFL
                     FGNSPDYRSVCYSAGINPDHLRVRLEKYK"
BASE COUNT          760 a          924 c          906 g          774 t
ORIGIN      
        1 agagagggtc tgcagaccgc ggtgcgtatc ccactccagc aaattccatt ccagcggcgc
       61 atcatgattc cattcgcttc gttgtccgaa ttcgctgccc atgaacatta gcttctttcc
      121 ggggtgcgca atcatgtagg caaaaaacag gcgcatattg gcaaactgtt gccagaggtc
      181 gccgggcatt ttattgatca gggaaccttt gccgtgaacg acttcgtcgt gagaaatcgg
      241 tagcacaaaa ttttcagtga aggcgtacag catggaaaaa gtcatgcggc tgtgatgata
      301 tttgcggtaa atcggatcca cggcgaagta actgagcgtg tcgttcatcc agcccatgtt
      361 ccacttcagg ctgaatccca agcctcccaa atatgtgggc cgcgacacca tgggccagga
      421 cgtcgattct tccgcgatgg tcaggacgcc cgggtgcctc gcgtaaatca cttcgttcaa
      481 gcgcttgata aaagcgatgg ccgcgagatt ttcccgtccg ccaaattcgt tgggaaccca
      541 ttcgccttcc ttgcgcgaat aatcgaggta caacatggac gccacagcgt ccacgcgcag
      601 cccgtcaatg tgatatttgt cgatccaaaa aagcgcgttc gatatcagat agttttgaac
      661 ttcgtttcgc ccgtaattaa atacgagcgt tccccagtcc ggatggcgcc cctgccgcgg
      721 gtccgcatgt tcgtacagat gggtgccgtc gaattcgccg aggccgtggc cgtcgctggg
      781 gaaatgcgcc ggcgtccagt ccagtaaaac gcctatgccg gcttgatgaa accggtcgac
      841 gaacgccatg aaatctgcgg gcgtgccata gcggctagtg gctgcaaaat atcccagcgt
      901 ctggtagccc cacgagccgt cgaagggatg ctccatcacc ggcatcagct ccacgtgcgt
      961 gtaattcatc tgctttaagt agggaatcag ctgatccgcg agctcgctgt aagaaagcca
     1021 gcgccatgga tcctgcgcgt tgcgccgcca cgagccgaga tgaatttcgt acacagcgat
     1081 gggcgaagaa agccaatcgt ttctcgcgcg ccagtccatc cagcccgcgt cattccattt
     1141 gtggtgatcc aggcgcgtta cgatcgatcc cgtgttcggc cttagctccg cgcggaagcc
     1201 gtaaggatcg cttttcagca ggggtgcacc gcctgacatc ggacggattt cgtacttgta
     1261 ggtcgcgccc tccgcgagtt cgggcacaaa caattcccaa actccggaag ggccgcgatt
     1321 gcgcatcggg ctaatgcgtc catcccactg attgaaatct cccaccacgc tgacgcgcat
     1381 tgcgtttggc gcccacacgc taaattgcac gccggcaact ccgtcaacaa cctctggatg
     1441 cgcgcccatc ttttcgtact tctgatagtg cgttccttcg cccatcaggt acaaatcgaa
     1501 atcggaaagc aacggaggga atgcgtacgc atcatagatt tctcgcgcgg gccggccgac
     1561 ctcctcaagt ttccatacgt agctgctcgg tgggatcggt agttcaggag ctagcgggag
     1621 gacggcttca aataatccat ccgcgtgcac acgagcggcc ggaattgggt ccgacgcgcc
     1681 tcttgggagg acggaagcgt acgctgcatg tggaaaaaag ccgcgaacaa cgaggcgcct
     1741 gttctcatgt tcctccacgg tctcttccac gatgtgcgga ccgagcaggc ggaacgggtc
     1801 tcgattttct gcccgcagca gaaggccgag atctctgtcg aattcgctta ccggttcaag
     1861 gtccgccggc agttcctctg gtgatggatt tactgtgctc aactcgtcct cgatctacgg
     1921 agaaacagct gccctatgcc cagcattcta tcaggggcaa tgccgcttgg gagtaattta
     1981 gataaaaact ggcgacccac aaggcctgct caggggcatg tttaacccgc cgcgggagag
     2041 aaaaatgctt ccttccctga ccactaagag aaacagttct tcacatttta atacacgaat
     2101 attcacagaa aaccataacc ctattgtaat cagtacgtta tttgattact tttatggcac
     2161 aaggcttgca ttgtttatgg tgcaagcgca gtggattgcc ttaatgcggc cctggggccg
     2221 ctaacgctgt gggccaaagg tcgcctatga caacctcgtt tccgacgatg ctcagcctcg
     2281 caagaatcat tcccatcggt ggtctggttc tggcttttac ctgcagcccc gcccgagcac
     2341 aaagctcgct ttctggaacc tataattgcg tgagcgtgga agtgaacgga acagctcacc
     2401 cttgcaaagc gccttccatc gaaatgaatt ctgacggcag ctaccagatc ctggcggaac
     2461 gcggcacgta cgaaattcta aagggtcact ggctcgcgct gtcgacgtcc aaaaatcatg
     2521 ggcgggcgcg gcttgacggt agccgcacga tcatctttga attcgaatcc agcgggaaga
     2581 aaagtaagat cacataccgc cgcaaatacc agcggccgcc cgcgtgggtg tctagttagg
     2641 cgttgaagtt ggaagtggtg gattgctcgc ggcgcgaaaa ggtgctgctt gctttcagtg
     2701 ctcgccggtt cggtgtccca cggtgttgtg acgacctggt acaagtctat tgctgttgtt
     2761 tgctattgaa actgtgctga cttttgcccg tttgttgtcc ctcaacgacg ttcctctaac
     2821 taccctgatt acaattccct gcaaggaata aaaaattccg ccctgccagc ctgttgggct
     2881 cccagttgcc tttacagaag tgcttgcgat tgagcgaacc gtttttcgca agcaaaaaca
     2941 aacttactgg gggggtaaca tgagcatctt tgaacagggc gatgtggaag aagtaccggc
     3001 acagagattg tggcgcgcgg tgatcgcgac tacggttcag gagtgggtca gcggaccgct
     3061 gcgcatgaag cgtgaagcgg aaaagttttt gtttggaaat agcccggact accgctccgt
     3121 gtgctattca gcaggcatca atcccgatca tctgcgcgtc cgcctggaaa aatataaata
     3181 attccagcgc gccttttttg cttaggcgtc gctgttttgt tgacactcga attcgcgttt
     3241 gtataggccg tcagcacttc gggacagtga ctttcgtgtt ccatgctgaa gtgaaaatcg
     3301 caaatatatt cagcaagcga gccgaacaca cgacagtgta tcgctcgcgc ggtttgacga
     3361 acca
//
LOCUS       DASZMB010000233         7150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2971899, whole genome shotgun sequence.
ACCESSION   DASZMB010000233 DASZMB010000000
VERSION     DASZMB010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 7150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7150
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2971899"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(419..2956)
                     /locus_tag="VG272_10990"
     CDS             complement(419..2956)
                     /locus_tag="VG272_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14252.1"
                     /translation="MPERTFMLSTDTPARINLKVYSAELSLRTLAVGVDNIHHDVFLS
                     PAFVESTEAYLLEFIRQTVNLPFLTQKDRLPTDRRTTPRREGDKKSVRPADAASWKKQ
                     LSDLLHAGLQKAKFEKNIEIDLILRATLLKYLTQEIGAQFANILLEAKEWIRGRGEHF
                     DRSEQAHVIKAHLAELQADRRNLFRKVGQHVYQAILDIEDNSLGRSRKALFGEEAVAS
                     YDILNNRLAFVENGKDDVMFLEQYVLLGNYIRDQDRFETFDAVFLDLLRESVVTGDSG
                     SDLNETWQSHQKLVDAAVAYQAEIARLKEERETLMRKLERSESLMGRVGFGSDPASIR
                     ASITDVEKRQRHARRKLEETNPRIEASQSEADYQAKKYQDQLGDFLNQPENAQRLFDP
                     AALGTANRGKAGLRAQLLEELVARLEQRDLLVHILASYQLRNICHDYCPPIHLQQLKK
                     ALVSREELKRVEEILKQFPARQYSAARIEELSKKIRKTPREEVRAVVVRFAEDFMRLR
                     RDTRNYERLSAAMERVNLVRNERRRELSEMNHSLYEFLLPEEARPAEDHVVTHTVIKA
                     DVRGSTKITQELLARGLNPASLFSLNFYEPVKRILDRYGASKVFIEGDALVLAIYETE
                     STRANQRAVSKACALARQILAVSTAYNEKPDAADLPRLELGVGIAFQGSAPTYWMDTD
                     SRIMISRALNLSDRLSSCSKAAKRMLAENSSPFRLFLFQTMMEDVAEDEADEFLVRFN
                     LNGVELNEEGFQKLREEISLTELEADCIMPWGKERVTFFRGETPVGEGLEPVLIRRGF
                     IREMLPGGKIGQAGTRPYYEVCVSPKVLELANLLAESAEERREKMAG"
     gene            complement(3185..3811)
                     /locus_tag="VG272_10995"
     CDS             complement(3185..3811)
                     /locus_tag="VG272_10995"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HWF14253.1"
                     /translation="MNRTTRIPGLPVVCAWALAMLLFGASQSAVTAQSAADVSATSPA
                     PATIQDVHDTPVPLPAPQQPLPYSHKAHLALGLQCTECHTNPDRGRLMTFPDTATCMK
                     CHASFGKNRPTIQKLIEYDKARTTIPWVRIYTVLPGVKWSHRTHLDAGMKCEMCHGNV
                     SQMQVMTNVKSVNTMVGCLDCHALHNAPTTCTTCHPAWEPEMGVNKKK"
     gene            complement(3909..5564)
                     /locus_tag="VG272_11000"
     CDS             complement(3909..5564)
                     /locus_tag="VG272_11000"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWF14254.1"
                     /translation="MTDITRRGFVKGAAVAPLAFSPFTMAQTSSAPHYDIVVVGAGHN
                     SLITACYLAKAGFKCLVLEGRPKIGGGTKTAQLTLSGFEHDVCSSIHARIQANPLMKN
                     NELNLGEYGLEYIFPDPVYHIAFPDGSYITKWRDFDRTCAEIAKYSKKDAETWRRMAN
                     ESDEIRSIVNQVEYTPIGFGKSLAERLAEHPKGKVWQRRMAMSKWDVLHQNFEDERTM
                     VAMMPPFPKELTGPYSGFSAYPSKKEEVPQPKGGSGMIATALQRFLEARGGEILVNKP
                     VEQLIVENGKCVGVECADGSSYRAEKAVLSTVHIKQLVNMAPKEMWPQDFLEGVKTFN
                     TGNASFNSHFATTEPLKFPTKEGTVVPVHISNIQSCKRALRYESEIIAGEFHVEEPVL
                     HIVQTSVADPTRAPAGMHTIRVLCEQAIYDLPDGGPGRWAEIKAEVQLAHLRAVQKIA
                     PNLTDDKILARFSTSPVDIEQMNAAMWHGSCHSGTDGPAQAGAMRPVPGWSQHRMPIP
                     GLYQTGGTTHPGGGVSGGPGRNAAVVMLKDFGTSMEEVLSKKG"
     gene            complement(5611..6567)
                     /locus_tag="VG272_11005"
     CDS             complement(5611..6567)
                     /locus_tag="VG272_11005"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWF14255.1"
                     /translation="MTNRFVVCAAMILIGSVVAVGFGSSAGSFPQSAPSAPVPIKFDH
                     ALICVSDLAPMQQALADVGLKPDYGGHHGHATTQMAQLGFADGSYIGVEAPVDASVET
                     GTPQSALMKANAGPCGWSATSTDMKGDLERISKLGVKVGEPEVRSRTRPDGALAEWHS
                     ASLGAGAPGSNLPFLIEDKGPRSNRAPHASPSTEGTTISGVDIVVLGVKNLDDSIALF
                     RKVYSLNAPAVEDHPEFGAKIAYFPGTPVMLAAASSPDSWVAERVGRLDQIPVAFLLH
                     ADDFKAASKHFSLPEGKAWFHQDVSWFDVQKLRGVRLGIIGG"
     gene            complement(6698..>7150)
                     /gene="tsaE"
                     /locus_tag="VG272_11010"
     CDS             complement(6698..>7150)
                     /gene="tsaE"
                     /locus_tag="VG272_11010"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HWF14256.1"
                     /translation="TNSYEETIAQGREIAAKLVPPVLVLLKGDLGAGKTTLTKGIISG
                     LGAAREEEVTSPTFNLVHEFRIQNPGADGANARGCKVYHVDLYRIDSFHDLESLGIED
                     ALSENAIVIIEWPERFTFRTDWPTIEIHLEHAGGDVRKITVMDLEATV"
BASE COUNT         1490 a         2084 c         1875 g         1701 t
ORIGIN      
        1 cacgttcatt agtaggacgg gcttccgcct gtctgctcta ccaatcgttt aaaagcgtaa
       61 atcatggatt gcgctaacag attcctagct tcgctcggaa cgacaggcct ctcttactta
      121 atgatggtcc ccgctgacca cggcgtgatc gaacaaaact ctaaaccaga caggctgaag
      181 cctgtcctac tattcctgaa tcaatttctt tttgtgtttg gttggcttta ggcgtttatc
      241 ggggatcact cttgctgcgg gcggcgtccc ggcgttttca cgcgcacgcc tgcgagcttc
      301 gccggatgct gtgaactttt tgggcttgga tctggatttg cttctgctca ttaaattgaa
      361 acttgcggaa tgtattataa atcacagaga acgaacactg ggagggtttt aacccgcttc
      421 agccagccat tttctcgcgg cgttcttccg ccgattcagc cagcaaatta gcaagctcga
      481 gcactttcgg gctcacacaa acttcgtagt agggacgtgt gcccgcttgt ccgatttttc
      541 ccccgggcaa catttcacga ataaatccac ggcgaatcag cacgggctcg aggccttcac
      601 ctaccggagt ttctccgcgg aaaaaagtga cgcgttcctt gccccaaggc atgatgcaat
      661 cggcttccaa ttcggtcagc gaaatctctt cgcgcagctt ttgaaagcct tcctcattca
      721 gttccacgcc atttaaatta aagcgcacga ggaactcgtc ggcctcatcc tcggccacgt
      781 cctccatcat ggtttgaaac aggaagagtc gaaacggcga tgaattttca gccagcatgc
      841 gtttcgcggc cttagaacag ctggaaaggc ggtcggaaag attcaaagcg cgggagatca
      901 tgatgcgcga atccgtgtcc atccagtagg ttggcgcgga tccctggaac gcaattccca
      961 ctcccagctc caatcgcggc aggtccgcag catccggttt ttcgttgtaa gccgtggaaa
     1021 cagccagaat ctgccgcgca agcgcgcagg ccttggaaac ggctcgttgg ttcgcgcgcg
     1081 tcgattccgt ttcataaatc gccaaaacca gcgcgtcacc ttcgataaaa acttttgacg
     1141 cgccgtaccg gtcgagaatt ctcttcactg gctcgtagaa gttcaaactg aaaagtgaag
     1201 ctggattaag tccacgcgcc aaaagttcct gcgtaatttt tgtcgatccg cgcacgtccg
     1261 ctttgattac ggtgtgcgtc accacgtgat cttctgcggg cctcgcttct tcaggcagca
     1321 gaaattcgta gagactgtgg ttcatttccg aaagctcgcg tctgcgttca ttgcgcacca
     1381 ggttaacgcg ctccatggcc gcgcttagcc gctcgtaatt ccgggtgtcg cggcgcaacc
     1441 gcatgaaatc ttccgcaaat cgcaccacca cggcgcgcac ttcttcgcgc ggagtcttgc
     1501 ggattttttt ggaaagttcc tcaatgcgcg ccgcggaata ttgccgcgcc gggaactgct
     1561 tgagaatttc ctccacgcgc tttagttctt cgcgtgagac aagtgctttt ttcagttgct
     1621 gcaagtggat gggcggacaa tagtcgtggc aaatattccg cagctggtag ctcgcgagaa
     1681 tatgcaccag cagatcgcgc tgttccagac gcgctacgag ttcttccagc aactgggcgc
     1741 gcagcccggc cttgcctcgg ttcgccgtgc caagcgccgc aggatcgaac agacgctgcg
     1801 cattttcggg ctggttcaga aaatcgccaa gctgatcctg atattttttt gcctggtaat
     1861 ccgcttcact ctgcgaagcc tcaatgcgcg ggttcgtctc ttccaacttg cgccgggcgt
     1921 ggcgttggcg tttttcaacg tccgttatcg atgcgcgaat ggaagcggga tccgagccga
     1981 aaccaacgcg ccccatcagg ctctcggatc gttcgagctt gcgcatgaga gtctcgcgtt
     2041 cctctttgag ccgcgcaatc tccgcctggt aagccaccgc cgcatcgacc agtttctgat
     2101 gcgactgcca tgtttcgttc aaatcactgc ccgaatctcc ggtcacgacc gactcgcgca
     2161 atagatccag aaacaccgca tcgaacgtct caaatcggtc ctggtctcgt atgtaattcc
     2221 cgagcagcac atattgttca aggaacatca cgtcgtcctt gccattttcg acgaaggcca
     2281 ggcggttatt caaaatgtcg tacgacgcga ccgcttcctc gccgaacagc gcttttctgg
     2341 acctgcccaa gctgttgtct tcaatgtcca gaattgcctg gtacacatgc tggcccactt
     2401 tgcggaacag attgcggcga tccgcctgca gttccgcgag gtgcgcctta atcacgtgcg
     2461 cctgctcact gcgatcgaag tgttcgccgc gcccgcgaat ccattccttc gcttccagca
     2521 gaatgttcgc gaactgcgcc ccaatttcct gggtcagata tttcagtaaa gtcgcgcgca
     2581 aaatcagatc gatctcgata ttcttctcaa acttggcttt ttgaaggcca gcatgcagca
     2641 ggtccgaaag ctgctttttc caggaagccg cgtccgctgg acgcacgctt tttttgtcgc
     2701 cttcccggcg cggggttgtc cgtcgatccg tcggcaagcg gtccttctgc gttagaaacg
     2761 ggagatttac cgtttgacgg atgaactcca gcaggtacgc ttccgtgctc tccacaaacg
     2821 ccgggctaag aaaaacgtca tggtgaatat tatcgacacc cacggccaac gttcgcagcg
     2881 aaagctcggc ggaatatacc ttcaggttga ttcgggctgg cgtgtcggtc gagagcatga
     2941 acgttcgttc gggcatatcg gcatcccaat gcgccatttg aggttagcaa gtgggaagtg
     3001 ggcggacaag gtgacgaatc caacccgtta acagcagatc ggacattcgg cggaattctg
     3061 cacttttagt tatcagcggg tatgcatgac atcaggagtt ttcggaaaaa cgattttttc
     3121 gagtgtggga ctacacgaat gattcaaaat gaaacatagc ggagggattt ctatctgccg
     3181 tagcttactt cttcttattc acgcccattt ccggttccca cgctggatgg cacgtcgtgc
     3241 aagtagtcgg cgcattatgc aacgcgtggc aatccaggca tccgaccatg gtattgacgc
     3301 tcttcacgtt ggtcatcacc tgcatttggc tgacatttcc gtgacacatt tcgcatttca
     3361 tgcccgcgtc caaatgggtt cgatggctcc atttcacgcc cggtaaaacc gtatagattc
     3421 gcacccacgg aatcgtagtt ctcgctttgt catactcgat cagtttctga atcgtgggcc
     3481 ggttcttacc gaacgacgcg tggcatttca tgcaggttgc cgtgtcgggg aacgtcatca
     3541 gccttccgcg gtctggattc gtgtggcatt cggtgcattg caggccgagc gccaagtggg
     3601 ccttatggct atacgggagt ggttgctgcg gcgcgggaag gggcacggga gtgtcatgca
     3661 catcctggat ggtcgccggc gcgggcgatg ttgctgaaac gtcggctgcg gactgtgcag
     3721 tgacggctga ttgcgaggct ccgaacaaca gcattgccag cgcccaggcg cacacaactg
     3781 gcaagccagg aattcgggtc gttcggttca caagacactc tccgtaagat gatccgtagt
     3841 atacacatgc tcgcctgaag gcgagcgtta caactgcatg gattgccggg attcgactcg
     3901 cgtcctgctt atcccttttt gctcaaaacc tcttccatgc tcgtgccgaa atccttcagc
     3961 atcacgaccg ctgcattgcg gccggggccg cctgagactc cgccgccagg gtgcgttgtg
     4021 cctccggttt gatacaaacc tgggatcggc atgcggtgct gcgaccatcc gggaacgggg
     4081 cgcatggctc cggcttgcgc cgggccgtct gtgccgctgt ggcaagagcc gtgccacatc
     4141 gcggcattca tttgctcgat gtcgacaggc gacgtcgaga agcgcgccag gattttatca
     4201 tccgtcaggt tgggtgcgat tttttgcacg gcgcgcaaat gggccagctg aacttccgct
     4261 ttgatctctg cccagcgtcc gggaccgccg tcgggcaaat cataaattgc ctgctcacaa
     4321 agaactcgaa tcgtgtgcat tccggctggg gcgcgtgtcg ggtccgcgac gcttgtctgc
     4381 acgatgtgca acaccggctc ctccacatgg aattcgccag cgataatctc agactcatag
     4441 cgcagggcgc gcttacagct ttgtatattg gaaatgtgca ctggaacgac tgtgccttcc
     4501 ttcgtcggaa acttcaaagg ctccgtggta gcgaaatgag aattgaacga ggcatttccg
     4561 gtattaaatg ttttaacgcc ctccaggaaa tcctgcggcc acatctcctt gggagccata
     4621 ttcaccagct gcttgatgtg cacggtggag agcacagctt tttccgctcg atacgagcta
     4681 ccgtccgcgc attccacgcc cacgcacttg ccattctcaa cgatcagctg ctcgaccggc
     4741 ttgttcacca gaatttcgcc gccgcgcgct tcgaggaaac gctgcagcgc ggtagcgatc
     4801 attcccgatc cgcccttagg ctggggcact tcttctttct tgcttgggta tgccgagaag
     4861 ccggaatagg gcccggtcag ttctttggga aacggcggca tcatcgccac catggtccgt
     4921 tcatcctcaa aattctggtg cagcacgtcc cacttggaca tggccatgcg ccgctgccag
     4981 actttgcctt tgggatgctc cgccaaacgc tccgccagcg attttccaaa tccgatgggt
     5041 gtgtattcca cctgattgac gatcgacctg atttcgtcgg attcattggc catgcggcgc
     5101 caggtttccg catctttctt ggaatattta gcgatctccg cgcacgtgcg atcgaaatct
     5161 ctccacttcg tgatgtagct gccgtctgga aatgcgatgt gatagacggg atcggggaaa
     5221 atatattcga ggccgtactc gcccaaattc agttcgttgt ttttcatcag cggattggcc
     5281 tggatgcggg cgtgaatcga ggagcacaca tcgtgctcaa aaccgctgag agtgagctgt
     5341 gcggtctttg tgcctccgcc aatctttggg cgtccttcga gcacgaggca cttgaagccg
     5401 gccttcgcca gatagcatgc ggtaatcagg ctgttatgcc ctgctccgac gaccacaata
     5461 tcgtagtgcg gcgcggaaga agtctgcgcc atggtgaaag gcgaaaaggc gagcggagcg
     5521 accgctgccc cttttacaaa tccgcgccga gtaatatccg tcatctgatt ccccctctgc
     5581 gagcgcaccc tgcgttaatg cttttaaaaa ttatccgcca atgattccca atcttactcc
     5641 acgcaacttt tgcacatcga accaagaaac gtcctgatga aaccaggctt ttccttcggg
     5701 cagcgaaaaa tgtttcgacg ccgctttaaa atcatccgca tgcagcaaga aggccaccgg
     5761 aatttgatcg agccgaccaa cccgctccgc cacccaggag tccggcgacg acgccgcggc
     5821 cagcatcacc ggcgttccag gaaaataggc gatcttcgcg ccaaactcgg gatgatcttc
     5881 cacagccggc gcattcaggc tgtacacttt ccgaaagagc gcaatactgt cgtccagatt
     5941 tttcactccc agcacgacta tgtcgacgcc ggagattgta gtgccctctg tgctgggcga
     6001 agcatgcgga gcgcgattgc tgcgcggccc tttgtcttcg atcaaaaacg gtaggttcga
     6061 tcccggtgcc ccggcaccaa gcgaagcgct gtgccattcc gccaaggccc catcaggacg
     6121 cgttctgctg cgcacttcgg gctctccaac ctttaccccc aacttggaaa ttcgctcgag
     6181 gtcgcccttc atatcggttg acgtcgccga ccaaccgcac gggcctgcgt tcgctttcat
     6241 gagcgccgat tgcggcgtgc cggtttccac ggacgcatca accggcgcct caactccaat
     6301 gtaactgccg tccgcgaatc caagttgagc catctgcgtc gtggcgtgcc catgatgccc
     6361 gccgtaatca ggtttcagac ccacatccgc cagcgcttgc tgcatgggcg caaggtcgga
     6421 aacgcaaatc agcgcgtgat caaatttaat ggggacagga gccgagggcg cagactgcgg
     6481 aaagcttcct gcgctggaac caaaaccgac tgcaacgaca gatccgatca gaatcattgc
     6541 cgcgcagact acaaacctgt ttgtcacgaa cgagcccccg accgcaattt ttcgctgggc
     6601 caataaattt atttatctag aaggctatac tcgcggagag ccgaaataca aatgaattct
     6661 ggtgctgatt tcaagcttcc ccaagttgtt acgcagacta taccgttgct tctaaatcca
     6721 tcactgtgat cttacgcaca tccccgccgg catgttccag atgaatttcg atcgtcggcc
     6781 aatccgtgcg aaacgtgaat cgctccggcc actcgattat cacaatcgcg ttttcggaca
     6841 acgcgtcttc gatgcccagc gattcgagat catgaaagct gtcgatgcga tagagatcga
     6901 catggtaaac cttgcacccg cgcgcgttcg ctccgtctgc tcccgggttt tgaatccgga
     6961 attcgtgcac cagattaaac gtggggctcg tcacctcttc ttcccgcgca gctcccagcc
     7021 cgctgatgat gcccttcgtc agcgtcgttt tccccgcgcc cagatcgcct ttcaacaaaa
     7081 ccagaacagg cggcacaagt ttagccgcga tttccctgcc ctgggcgatc gtttcctcgt
     7141 aggaatttgt
//
LOCUS       DASZMB010000234        23458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3256268, whole genome shotgun sequence.
ACCESSION   DASZMB010000234 DASZMB010000000
VERSION     DASZMB010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 23458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23458
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3256268"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VG272_11015"
     CDS             complement(<1..488)
                     /locus_tag="VG272_11015"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HWF14257.1"
                     /translation="MRSKASFRISMHAAATIFLAATIALSGWSQAPTADDKSIPTSKV
                     ERMNRAPVSKDILKVKLPRPVETTLPNGISLMILEDHRFPLVTVMFDISGAGPMYEPA
                     AQPGLAGATARLLAEGTKTRTSKQIAEQIDSLGASLSASAGFGSSSAIVSASGLSDKF
                     DE"
     gene            complement(553..1944)
                     /locus_tag="VG272_11020"
     CDS             complement(553..1944)
                     /locus_tag="VG272_11020"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWF14258.1"
                     /translation="MKRFRVFSMAALALALGIAGMAVAATSRRIAAADSAAPSQEVKI
                     PIEQYTLKNGLRVVLSEDHAAPTVSLCVTYDVGSRNELPGHTGFAHLFEHMMFQGSEN
                     VGKGEHLILVRVNGGDVNGTTSDDSTNYYERVPANQIDMALFLESDRMKALRITQANL
                     DNQRSTVQEERRLRMDNQPYGKTNEIIEGLTYDNFAYKHSTMGSMDDLNAANLSDVAQ
                     FFKTYYAPNNAVLTLVGDFKSSEALAKIKKYFEDIAQQPAPPSVDMTEPAQTAERRVT
                     TEDSFARLPRLDIVYKTVPANTADFYALDMLGDILFSGPSSRMYQKLVKEKMLALQVA
                     GGMDLRRGPALFQAFALLKPGQNSGDVEKIIYEEFERIEKDGVTPEEMRKVLIQDRLQ
                     QAESMTSTMSRARTLGEYAVYFHDPDLINTILAKYSEVTPADIQRVAREYLGAARRSV
                     VLTTPKAQAAPAR"
     gene            2097..2909
                     /locus_tag="VG272_11025"
     CDS             2097..2909
                     /locus_tag="VG272_11025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWF14259.1"
                     /translation="METVPESNVIDVLSLTRRYGPLVAVDHLKFSVSSGATFGLLGPN
                     GAGKSTLIKMLTTLLPPTEGTAAIAGFDLVQHPELVRKKIGYVSQMLSADGDLTGYEN
                     LLISAKLYGMPRAERSRRIMQGLEFMDLAHVSGKLVRQYSGGMIRRLEIAQSMLHRPA
                     VLFMDEPSIGLDPSAKRAVWERIRELRREFGTTILMTTHDMEEADHLCDMVAVMHRGV
                     LAAMDTPAALKAQVGADASLDDVFIHFTGATISEGGELRDAARTRITSQRLG"
     gene            2869..3690
                     /locus_tag="VG272_11030"
     CDS             2869..3690
                     /locus_tag="VG272_11030"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF14260.1"
                     /translation="MPLAQESHLSAWAEPFPGANYLRQTFAVAEADIRKLVHDPVELF
                     TRMVQPVLWLLIFGQVFNRTRAIPTGGISYLDFMAPGILAQSVLFGAIFYGISLIWER
                     DLGIVQKYLVSPAPRSTLVLGRAISSTVRSLCQMVFVYGLSYVLHIQLRFQVFALAGV
                     VLAVMLGSAIFSTFSLIVACLVKSRERFMGIGQVLTMPLFFASNAIYPLSLMPGWLRV
                     ASRGNPLSYEVDALRTLMIVGSTTSFSLGTDFLMMFAAFVVMVVIATKLYPTIVT"
     gene            complement(3893..7201)
                     /locus_tag="VG272_11035"
     CDS             complement(3893..7201)
                     /locus_tag="VG272_11035"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HWF14261.1"
                     /translation="MSRIAALFVSNSKTRFSARTFPGFLFFLAGGLLAAILPCAPATS
                     AQVSASISGRVTDPTGATVTGAAISTKNIETGETRSAITDDAGRYYVPSLAVGEYEVH
                     AKKQGFQEQVRSGIHLVVGQEASVDLALKLGEVTEQLKVMEDAPVVSVTAADISGVVG
                     EQQVKDLPLNGRSYDELMTLNPGVVTFTSEKTGGVGVSNSTNANMFSVAGNRPQQNLF
                     LLNGIEYTGAAENNMTPGGTSGQMLGVDAVREFNLLSDTYGAEYGKHPGGQVTIVTTS
                     GTNQWHGSLFEFVRNDIFDEPNFFDLGSAPPFRRNQFGGAIGGPIQKDKTFVFANYEG
                     FRESLHQTSVAFVPDAAARSGAFVPLGSACTAPNQAVCAAEVASLLNLWPVANGPDQG
                     SGIAIDNSNPLRSIREDFGTARVDHIISDKDSFGAIYTIDDSVDQDATAINPFMSDYT
                     TLREQVVSLEETHVVSPSFLNTARFGFSRAAYYFTGEPTPGTPAATVSPFLNGLPFIG
                     AVVVGGSAASNPAAQVALASSNNGSNLNLHRNLFTYEDQIAWTKGRHQFKFGAWFQRF
                     QSNENIALSQYGQATFPSLTNLLSGTFGTGSFLIDPSSTPLSWRALFGAIYAEDVIRV
                     GPSFTLSLGYRQEFSTGWNEAYGKASNYTFTNGVINTQPQIGNSVFTQNNARFLPQPR
                     IGLAWSPIGRKTVIRAGAGMYNDLQDALGYRTDQNAPFNATYTIPNLTLNKLPLSPNI
                     VPVSAGTASPAGTKIAPGGVDPNMKTPTIISYSLKIEQEISSNTSISVGYVGSHGSHE
                     LVSVDANIPTQVVCPASPCPGTLPSGTIFNTSTARQNPSLANTYSWFSEGDSMYNAMT
                     VDVRHRFSHGLTFRGAYTWSKALDDGDSLNATAAANAPALVQNPLNIRGDWGPATYDV
                     RNAGVMNVSYDLPFGNGRQMLNSGSGWENNVVGGWTLNSIVTIQSGFPFTPQLSFNPS
                     NDGDSKNPVRPSWNTSFSGPVVLGGTAQWFNPAAFVVPTSGTFGNVGRNVLTGPGLGT
                     LDFSVLKDTHLFERMNLQFRAEIFNLLNRTNLNTPNLIVLTSVGGAPSPVAGNFTQTS
                     TTSRQVQFSLKLLW"
     gene            7343..7597
                     /locus_tag="VG272_11040"
     CDS             7343..7597
                     /locus_tag="VG272_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14262.1"
                     /translation="MAGGGESYALPLQRRASVFHSQPGVRFHFLSVATKLSARNKIEG
                     KIVELEAEGALGPFRLVAPHEKRPARWVEMVKSITVVRVP"
     gene            complement(7688..8971)
                     /locus_tag="VG272_11045"
     CDS             complement(7688..8971)
                     /locus_tag="VG272_11045"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWF14263.1"
                     /translation="MRSRLLPFCTFLLLLLSVFGASPKLLAQYPQPGPAKLYAIKAGK
                     LVDPEKGATETNQIILVRGKKIEAVGANLQIPADAKMIDLSKSTVLPGLFDAHTHLCM
                     TLKKDRDGNNYYFTTLLDSTPYRAIEGVANARDMLAAGFTTVRDVGNAGNYADSALRE
                     AIERGLVPGPTMLNAGRIIAPYGGQFHLQPEKRDLATPEYAFADTHDEMIKAIRENIH
                     YGATVIKIVVDDQKYIYSADDIRFMVEEAHRDGLKLAAHCWTRAGAHNAAEAGVDSIE
                     HGEMMTNEDLQLAKKNHVVLVGTDFTESAARNSGMPDMHAVFVDRLKRAYQIGVTMAF
                     GTDSFVYVPGETRGTQAVEYVDSWVEAGVPAKDTLRAMTINAARLLGVDGERGTIKPG
                     LAADIIATPENPLDDIQAVRKVSFVMKDGSVFRNE"
     gene            complement(9055..9519)
                     /locus_tag="VG272_11050"
     CDS             complement(9055..9519)
                     /locus_tag="VG272_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14264.1"
                     /translation="MPEKLNRRQLMQAAAGASLAAVALSHGLPLQGAQNSNPAPHVPG
                     VDMSHECTVEDPEHYHLEFENEFVRVIRCRIPALDKVKMHNHPLGSVIIFMTDQNLHQ
                     TLESGKTEEAHDKAGHVLWANGSQSQHMGENVTDQMYEYLRVDVKAAFPKRA"
     gene            complement(9622..12291)
                     /gene="acnA"
                     /locus_tag="VG272_11055"
     CDS             complement(9622..12291)
                     /gene="acnA"
                     /locus_tag="VG272_11055"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HWF14265.1"
                     /translation="MAPASKNSFSTQESLKVDNKPYEIFRLDALEKKGVGHVSKLPFS
                     LRVLLENLLRQEDGKFVHPADIEALAFWDPKAATQKEISFMPARVLLQDFTGVPAVVD
                     LAAMREAIVKLGGDPKKVNPLLPAELVIDHSVQVDKSGSDMAFAFNAELEFQRNVERY
                     GFLRWGQDALNNFKVVPPDTGICHQVNLEYLARVVFDAAADGKTYAFPDSLVGTDSHT
                     TMINGLGVFGWGVGGIEAEAAMLGQPLSMLIPEVVGFKLHGRLPEGTTATDLVLVVTE
                     MLRKKGVVGKFVEFYGTGLSSLSLPDRATIANMAPEYGATMGFFPVDAETLAYLTFTG
                     RSKERVRLVEEYTKAQGIFRTDATPDPMYTDKLELDLATVEATLAGPKRPQDRVPLRE
                     SKASFEKSMEGAAQKHVSVRENGDKFELSNGSVVIAAITSCTNTSNPSLMLGAGLLAK
                     KAIERGLLSKPWVKTSLAPGSKVVTDYLEAAGLGKYLNELGFQLVGYGCTTCIGNSGP
                     LEEHIGTAIKENSLVAVSVLSGNRNFEGRIHPLVRANYLASPPLVVAYALAGTMDFDM
                     ATEPLGDDKAGKPVYLRDIWPTPQEIESTVRASVTTAMYNKEYGEVFKGDSRWQELKV
                     PEGDIYAWDEKSTYIKNPPFFDKITKTPAPLTDIHNARALAMLGNSITTDHISPAGSI
                     GVDTPAGKWLIAQGVKPVDFNSYGARRGNHEVMMRGTFANIRLRNQLAPGTEGGWSVH
                     QPDGEKMSIYDASMKYQKEGTPLIVLGGKEYGSGSSRDWAAKGTVLLGVRAVIAESFE
                     RIHRSNLLGMGVLPLQYRAGESTQSLGLTGFEEFTIEGISKLAPGMTVEVRARDKSGK
                     ETVFQTVARADTPEEVNYYRHGGILPYVLRQML"
     gene            12525..12863
                     /locus_tag="VG272_11060"
     CDS             12525..12863
                     /locus_tag="VG272_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14266.1"
                     /translation="MKKLILAFTVIACALTISTGTKAQDAPKVHSMTGCLRAGTAPDT
                     YMLTDLEKGPKSVGIVSSSAKLAPHVGHKIEITGTAVPPAEAEAMKDVPKAPHYMKVT
                     GVKMISTTCP"
     gene            13039..14493
                     /locus_tag="VG272_11065"
     CDS             13039..14493
                     /locus_tag="VG272_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_223146968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM protein"
                     /protein_id="HWF14267.1"
                     /translation="MKILLISTYELGRQPFGLASPAAWLRGGGHSVVSLDLTRETLED
                     EQILAAELICFYVPMHTATRLAAGLIPRVRKINPQAHLCFYGLYAPVNEEYLRGLGVG
                     TILGGEFEEGLMSLAQRSQQGAANGNSPGAQAEPVISLARQKFLVPDRAGMLDPAKYA
                     RVVMPSGEHRVAGSTEATRGCKHLCRHCPIVPVYNGAFRVVEREIVLEDIRRQVAAGA
                     RHITFGDPDFFNGPTHSISIVRAMHRDFPELSYDVTIKVEHLRLHDALLPVLRDTGCL
                     FVTSAVESVDDAVLEKFDKGHTRADFLAVIARFRELSMTLIPTFVPFTPWTTFSGYLD
                     LLDVLSTVGLTENVPPIQLAIRLLIPAGSRLLELAEVKAIIDPFDSSALVFPWTHADA
                     RMDALAREISQIVQRGDGLKLSRTEIFSHIRRAAQAASGNRTPVVGAASLAVKSAPVP
                     YLDEPWYCCAEPMDAQFVSIGKPKEAVAKADQYV"
     gene            14809..16092
                     /locus_tag="VG272_11070"
     CDS             14809..16092
                     /locus_tag="VG272_11070"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HWF14268.1"
                     /translation="MSRLLILASKLGYQTRSFAEAARALGVDVVFASDRCHQLEDPWA
                     DGAIAVRFDEPEESARQIVKQSFVRPVDGLIALGDRPAATAAHVARALGLSFHSPQAV
                     ENCRSKLRQREVLRDAGLPVPGFFSFKLDKRVEKILPRVQFPCVVKPLRLAASQGVIR
                     ANNPEEFQAAVARIKQLLESPEVKVTREPELDRLMVERYIPGREVAVEGLLTHGKLRI
                     LGVFDKPDPLEGPYFEETIYVTPSRLPDEMYDRVLECAERTVKALGLTHGPVHAEFRI
                     NDEGSWVLEAAPRPIGGLCSRALRFGPQRMLLEELLVRHALGMQGTDIAREEDASGVM
                     MIPVPKSGVLEKVEGEEQARATAGIEDLQITARSHDVIAAWPEGASYLGFLFAHGKTA
                     AEVESALRAAHAVLKFEITERLPVQHPVTGVAGTA"
     gene            complement(16405..16743)
                     /locus_tag="VG272_11075"
     CDS             complement(16405..16743)
                     /locus_tag="VG272_11075"
                     /inference="COORDINATES: protein motif:HMM:NF003817.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HWF14269.1"
                     /translation="MADDSCGPVKPAAPSTTAKPGQRMVHCVKFDKDMPGLERIPWKG
                     EIGKRVFENVSADAWKLWIEYSKMLMNEYRLNPLDPQSQKIMEEQMEQYFFGEGAKLP
                     EGYVAPKAKG"
     gene            16914..17534
                     /locus_tag="VG272_11080"
     CDS             16914..17534
                     /locus_tag="VG272_11080"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HWF14270.1"
                     /translation="MSDVTAIFWDVGGVILSNGWDRNARAAAAKKFGIDWEELQDRHD
                     LASPAFETGRITLDTYLQRTVFYRKRSFTKEEFTDFIFAQSREFPESRSVLSDVAATG
                     EYLQATINNEPRELNERRIHEFSLRRELKAFFSSCYLGIRKPDEGIYKIALEVTQRNP
                     DECLFIDDRELNLECARQLHMRTIHFQDAAQLRRDLIASGVSINES"
     gene            17549..18463
                     /gene="gnd"
                     /locus_tag="VG272_11085"
     CDS             17549..18463
                     /gene="gnd"
                     /locus_tag="VG272_11085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HWF14271.1"
                     /translation="MELGMVGLGRMGANMVERLLRRGHRVVGFDRDAGAVARVTAAGA
                     GGADSLANLAGKLGKQKAIWIMVPSGAPVDQTIAELIPHLSKNDILIDGGNSNYKDTQ
                     RRGVSLKEQGFQFVDVGTSGGIWGLKEGYSMMVGGDNAAIDYLRPIFETLAPGKDLGW
                     GHVGPSGAGHFVKMVHNGIEYGMMQAYAEGFAILKKKNEFALDMHQVSEIWRTGSVVR
                     SWLLDLLSIALKENAEMTGIAPYVSDSGEGRWTVAEAIDLDVPAPVITLSLLQRLTSR
                     DTDSYADKLLAAMRNQFGGHAIKAAAKK"
     gene            18474..20021
                     /gene="zwf"
                     /locus_tag="VG272_11090"
     CDS             18474..20021
                     /gene="zwf"
                     /locus_tag="VG272_11090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HWF14272.1"
                     /translation="MTSGKSATMERAELPKQSSITPADPCTIVIFGASGDLSRRKLVP
                     ALYGLAAQNCLARRFAIIGFARTPMTDEAFQQTAVDSVKKFADANTLREEECKEFAHA
                     LAYVSGEYHHPEAFEKLKARLDELDRAHKLNGNRLFYLATPPEIYPTIIEQLDRAGLS
                     RNPNGKSWTRVIIEKPYGRDLASARKLNESVRKAFDETQVYRIDHYLGKDTVQNLMVL
                     RFGNGIFEPLWNRNYVDHVQITAAETLGVEQRAAFYETAGATRDMIQSHVLQLTSLVA
                     MEPPAAFDATAVRNEKIKVLQSIRPFTPKSIKTDVVRGQYGPGSAPGNSSLLAGYREE
                     PGVAKNSGTETFVAASLQIDNWRWAGVPFYLRTGKRLPSRVTEIAIQFRKAPHLVFRG
                     QDLATNALVLNVQPDEGISISFHAKLPGQEMKLKTVTMDFSYQAEFGGGERSAYATLI
                     NDCMRGDATLFDRADGVEAAWALVDPILNYFEANKPKFPNYSAGTWGPHEADELLERD
                     GRQWRNS"
     gene            19969..20853
                     /gene="pgl"
                     /locus_tag="VG272_11095"
     CDS             19969..20853
                     /gene="pgl"
                     /locus_tag="VG272_11095"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HWF14273.1"
                     /translation="MKPMNYWSAMADSGEIRRGELPENPPEIQIFPNPADVARAAARF
                     FVDYAWQCIAKDGQFMVALSGGNTPRQMFEILASDEFRGQVDWAKVHVFWGDERAVPP
                     TNPESNYGMARRELLIRVPVPEGNVHRMEAEKASIGRAAHEYEEVLRKYLELDDQGFP
                     RFHLIFLGMGNDGHTASLFPGPRVTRQTSRWVSTPTVTKLNMRRMTLTLPVLDAALRV
                     VFLVVGSEKAQMLHAVLEEKHDPPYPAQLVQPRHRGLKIFLVDKAAAALLTHRGSKQP
                     ATDGKPAGATRGKTGRNM"
     gene            20850..21851
                     /gene="glk"
                     /locus_tag="VG272_11100"
     CDS             20850..21851
                     /gene="glk"
                     /locus_tag="VG272_11100"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HWF14274.1"
                     /translation="MILAGDIGGTKCNLALYDIHGVSPKKIFTQRYESRDFRTFDQLI
                     AKFLTDSQSESKIAGDMAVEAAGFGVAGPIINRRVKATNLPWIVDAAALATQLNTRHV
                     MLLNDLEATGYSLSHLAASEISILNRGAATPNLTQALLAAGTGLGETILFWDGSKYVI
                     SSSEGGHTDFAPRTEVEIELLRYMKKRNEFVSVELILSGRGFRTIHNFLNPSVEHPSF
                     EDPHADAAPEITRLGMAGQCPVCVQTLDLWVSMYGAEAGNLALKVLARGGVWVAGGIA
                     VKIQKKMEDGTFFRAFCAKEKFAELLAQIPIYMVLNEEAPLVGALSQAMKAAGIQLP"
     gene            complement(21873..22493)
                     /locus_tag="VG272_11105"
     CDS             complement(21873..22493)
                     /locus_tag="VG272_11105"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWF14275.1"
                     /translation="MNQKPNIFRQFSVFLTLSILAAMATLVSVQTARAQELSVQIDPE
                     QSKVQYSLGSTLHTVHGTFAVKNGSVHYDPSSGQIGGTIVVDATSGQSGNNSRDTRMH
                     REILESVKYPEIVFSPAKLTGVVAADGISRVEVSGRFRLHGQEHDVTLPLDVKVNGKT
                     LDITTRMNIPYVQWGLKNPSNFLVHVSDTVTVEIQARGHVQSTGNP"
     gene            complement(22499..>23458)
                     /locus_tag="VG272_11110"
     CDS             complement(22499..>23458)
                     /locus_tag="VG272_11110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWF14276.1"
                     /translation="QGVAALKTLDFSLPAEVAFPFRGIRFVDENNSVRADFADETGIS
                     VRRVKLHQLLVNHAMEAGVEFRWGARVTQIDKHTVTTPLERFSYEWLIGADGQNSQVR
                     KWAGLEPRATRRKRFGFCTHFNLKPWSDVAEVHWTNGCQIFITPMRGREVGVAVISRD
                     PQLRFDKAIRRFPFLAEKLCGVEPTTRELGDTTSLRILPKVTNGRVALIGDASGTVDA
                     VTGHGLSLSFQQAITLAESIEQRDLAFYESSHKKIASIGITMTRLMTLMSENDWIRRR
                     ILRLFQISPSLFARMLTIHTGTVPLSSVAMAEIADFSWKFLRA"
BASE COUNT         5106 a         6089 c         6709 g         5554 t
ORIGIN      
        1 cactcgtcaa atttatccga caaacccgac gcggacacaa tggctgagga ggaaccgaaa
       61 cctgctgagg ccgaaagcga tgcgcccagc gaatcaattt gctcggcaat ttgtttgctc
      121 gtgcgcgttt ttgttccctc agctagcaag cgcgctgtag ctcccgccag cccgggttgc
      181 gcggcaggct catacatcgg tcccgctccg ctgatatcga acatgacggt gaccagcgga
      241 aaccggtggt cttcgagaat catgaggctg atgccgtttg gcaatgtggt ttcgacgggc
      301 cgcggaagtt tcaccttcaa aatatctttt gagacgggtg cgcgattcat ccgctccact
      361 ttgcttgtgg ggatggactt gtcatccgcc gtcggggcct ggctccaacc ggacagggct
      421 atggtcgctg ccaaaaatat ggtcgcagcc gcgtgcattg aaattctgaa actggctttg
      481 cttctcatcg gtttcctcgt catcttcgtc atgctttgtt atgcacgcat cacgcttcgt
      541 tacgtggttg ggttatcggg caggggccgc ttgcgccttc ggcgtagtca aaacaactga
      601 ccgccgcgct gcgcccaggt actcgcgggc cacgcgctga atgtcggcgg gcgtgacttc
      661 gctatatttt gcgagaatcg tgttgatcaa atccggatcg tgaaaataga ctgcgtattc
      721 acccaacgtt cgtgcgcggc tcatcgtgct ggtcatggac tccgcctgct gcaatcgatc
      781 ctgaatcagg acttttcgca tttcttccgg agtgactcca tccttttcga tacgctcgaa
      841 ttcttcataa attatttttt cgacgtcgcc cgaattctgt cccggtttga gcagcgcgaa
      901 cgcttgaaat aaagcaggcc cgcggcgcag gtccattcct ccagccactt gcaaggccaa
      961 cattttttcc ttcaccagtt tttggtacat ccgcgaggat gggccgctga acaaaatatc
     1021 gcccagcata tccagcgcat aaaaatccgc cgtgtttgcc ggaacagttt tgtaaacgat
     1081 gtcgagccgc gggaggcgcg cgaacgaatc ttccgttgtg acgcggcgct cggcggtttg
     1141 cgcgggctcg gtcatatcca cggaaggagg agcgggttgt tgcgcaatat cttcaaaata
     1201 tttcttaatt tttgcgagcg cttcagacga tttgaaatcg ccgacgagcg tcagcaccgc
     1261 attattgggc gcatagtagg ttttaaaaaa ttgcgccaca tcggatagat ttgccgcatt
     1321 taaatcatcc atcgagccca tggtggaatg tttgtatgca aaattatcat aggtgaggcc
     1381 ttcgataatt tcattcgtct ttccataggg ctgattgtcc atgcgcaggc gacgctcttc
     1441 ctgcacggtg ctacgctggt tgtcgaggtt tgcctgcgtg atgcgcaagg ctttcatgcg
     1501 gtcggattcg aggaacagcg ccatgtcgat ttgattggcc ggaacgcgct cgtagtaatt
     1561 cgtggaatcg tcgctggtgg tgccgttgac atcgccgccg ttcacgcgga ccaggatcag
     1621 gtgctcaccc ttgcctacat tttcagagcc ctggaacatc atgtgttcaa aaagatgcgc
     1681 gaagcccgtg tgccccggca gttcgttgcg cgatcccaca tcgtaagtta cgcagagaga
     1741 gacggtgggc gccgcgtggt cttccgacag cacgacgcgc aatccgtttt tcagcgtgta
     1801 ttgctcgatg gggattttta cttcctggga tggggcggca ctatccgccg cagcgatgcg
     1861 tcgggacgtt gctgcaaccg ccatgcccgc aattcctagc gcaagggcga gcgcggccat
     1921 cgaaaaaact ctgaaacgct tcatgttgta ctccgccaaa catatttcga gctgacactc
     1981 tatcctacgt ttaatagacg tccgagggca ccaatagttt tgtacagcca tttcaagatg
     2041 gaccttgaaa gctcgctatc gcatcaaatc tttccgcggg gtaaactctt aggtccatgg
     2101 aaaccgtccc tgaatcgaat gtcatcgatg ttctttctct gacgcggcga tacggccctc
     2161 tcgtagcagt cgaccattta aaattttccg tttcatcggg cgcgacgttt ggcctcctcg
     2221 ggccaaatgg cgcgggaaaa agcacgctca tcaaaatgct tacgacgctg cttcctccca
     2281 cggaaggcac cgcggcgatt gcaggatttg atctggtcca gcatcccgag ctcgtgcgaa
     2341 aaaagatcgg ctatgtttcg cagatgcttt ccgcggacgg cgatctgacg ggctacgaaa
     2401 atcttcttat ctccgcaaag ctctacggca tgccgcgcgc ggaacgctcc cgccgcatta
     2461 tgcaaggcct ggaatttatg gacctcgcgc atgtgagcgg caaactggtg cggcaatatt
     2521 ccggtggcat gattcgacgt ttggaaattg cgcaatccat gttgcacagg cccgccgtct
     2581 tatttatgga tgagccatcg atcgggctcg atccctccgc gaagcgcgcc gtgtgggaac
     2641 gaattcgcga attgcgccgc gaatttggca caaccatcct gatgaccaca cacgacatgg
     2701 aagaggcgga tcacctgtgc gatatggtcg ccgtcatgca ccgcggtgtg ctcgccgcca
     2761 tggacacgcc ggcggcgctc aaagcgcaag tcggagcgga tgcttcgctc gacgatgtgt
     2821 ttattcattt cacgggggcc acgatttccg agggagggga actgagagat gccgctcgca
     2881 caagaatcac atctcagcgc ctgggctgaa ccttttcccg gcgcgaatta cctgcggcaa
     2941 actttcgccg tggcggaagc cgacatccgc aaactcgtgc acgatcccgt cgagctgttt
     3001 acgcgcatgg tccagccggt cctgtggctg ctaatcttcg gccaggtttt caatcgcacg
     3061 cgcgcaattc caacaggtgg catcagctat ctggatttca tggcgcccgg aattctcgcg
     3121 cagagtgttt tgttcggcgc gattttctat ggcatctctc taatctggga gcgcgacctc
     3181 ggcattgtgc agaaatatct ggtgagtccc gcgccacgga gcacgcttgt gctcgggcgc
     3241 gccatttcat ccacggtgcg cagcctgtgc cagatggtgt ttgtttatgg gctttcctac
     3301 gtgctgcaca ttcagctgcg gtttcaagtt tttgcgttgg cgggagtcgt tctggcggtg
     3361 atgctcggct cggcaatttt ctcgacgttc tcgctgatcg tcgcgtgcct cgtgaaatcg
     3421 cgcgagcgtt ttatgggcat cgggcaagtg cttacgatgc cgttattttt tgcgagcaac
     3481 gccatttatc cgctgagcct catgccgggt tggttgcgcg tcgcgtccag ggggaatcct
     3541 ttgtcttacg aggtggatgc gttgcgaacc ttgatgattg tggggagcac cacatctttc
     3601 agcctgggga cggatttttt gatgatgttt gcggcgttcg tggtgatggt ggtcatcgca
     3661 acgaaactct atccgacgat tgttacttga gggtttggcg atgcggcggc gaaattcaaa
     3721 ggtccaaggc tttaacacag agaaaagagg agaaaaccgg agaaagaatt ccgagacgag
     3781 aaacttgcaa ttgaatctcc cccgaaatga atgcctgatg ctctctccgc ctttctcctc
     3841 ttttctctgt gccctctgtg ttaaatcttt tccgtgtacg agtcctactt ggttaccaca
     3901 acagcttcaa gctgaattga acctggcgtg aagtggtcga tgtttgcgtg aaatttcccg
     3961 ccaccggaga aggcgcgcca cccacggaag taagcacgat caagttggga gtattcaggt
     4021 tcgttcggtt caacaggttg aaaatctccg cgcgaaactg gaggttcatc cgttcgaata
     4081 gatgcgtgtc cttcaagacg gaaaaatcca atgtgccaag ccctggcccg gtcagcacat
     4141 ttctgccaac gttgccaaat gttcccgatg tcgggacgac gaatgcggcc ggattaaacc
     4201 actgtgcggt gccgcccaaa acaacgggtc cgctgaaaga cgtattccaa gaaggcctca
     4261 cgggattttt ggaatcgccg tcgttggaag ggttaaagct cagctgcggt gtgaatggga
     4321 aaccagactg aatcgtgacg atgctgttga gtgtccatcc accaacaacg ttattctccc
     4381 aaccgctccc gctgttcaac atttgcctgc cgtttccaaa aggcaaatcg tagctgacat
     4441 tcatcactcc agcatttctc acatcgtagg ttgccggtcc ccaatcgccg cgaatgttca
     4501 agggattttg caccagagcc ggagcattgg ccgcagcggt ggcattcagg gaatctccat
     4561 cgtcgagtgc cttggaccac gtgtacgcgc cacggaaggt gaggccatgg ctgaatcgat
     4621 ggcgcacatc cacggtcatg gcgttgtaca tgctgtcgcc ttcagagaac cagctgtagg
     4681 tgtttgccag cgacggattc tgcctggccg tgctggtgtt gaagatcgtt ccgctgggca
     4741 aagttccagg gcagggcgat gcagggcaaa ccacctgcgt gggtatgttt gcgtccacgc
     4801 tgacgagttc atgcgatccg tgcgagccga cgtatcccac gctgatcgaa gtgttcgaag
     4861 aaatttcctg ctcgatttta aggctgtacg aaatgatggt gggagtcttc atgttgggat
     4921 ccactccgcc tggggcaatc tttgtacctg ccggcgaagc cgttcctgcg gaaactggga
     4981 caatgttggg agatagcggc aatttgttga gcgtcaaatt ggggatggtg taggtcgcgt
     5041 taaatggggc gttctgatcg gtgcggtacc cgagagcatc ttgtaaatcg ttatacattc
     5101 cagccccggc gcgaatcact gttttccttc cgatcgggct ccaggccaag ccgatccttg
     5161 gctgcggcag gaaccgtgcg ttattttggg tgaaaacgct gttaccaatc tgcggctgtg
     5221 tattgattac gccgtttgtg aaggtgtagt tagaggcttt gccgtacgcc tcgttccatc
     5281 cggttgaaaa ctcttgccgg tagcccagcg acagcgtaaa ggatgggccc actcgaatga
     5341 cgtcctctgc gtaaatcgcg ccgaacaaag cgcgccaact gagcggcgtg gacgagggat
     5401 cgatcagaaa acttccggtg ccaaatgttc cgcttagcaa gtttgtcagg ctaggaaacg
     5461 tggcctgccc gtactggctg agcgcgatat tttcgttgga ctgaaagcgt tgaaaccagg
     5521 cgccgaattt aaattgatgg cggcccttgg tccaggcgat ctggtcttca taggtgaaca
     5581 aatttctgtg caggttcagg ttgcttccgt tattgctgct ggcaagagcg acttgtgccg
     5641 ctggattgga ggcggcgctt cctcccacga ccaccgcgcc aatgaatggg agcccgttca
     5701 agaagggaga aactgtggcc gccggtgttc cgggcgtagg ttcacctgtg aaatagtaag
     5761 cggcccgaga gaagccaaag cgcgcggtgt tgagaaaaga gggcgagaca acgtgcgttt
     5821 cctcaaggct cacgacttgc tcacgcaggg tcgtatagtc cgacataaat ggattgatcg
     5881 ccgtggcgtc ctgatcgacg ctgtcatcta tcgtgtagat cgcgccgaat gaatccttgt
     5941 ctgaaatgat gtgatcgact cgcgccgtgc cgaaatcctc tcgaatcgac cgcaaaggat
     6001 tgctgttatc gatcgcaatt ccgcttccct gatccggtcc attcgctacc ggccacaagt
     6061 tcagcagtga ggccacttca gccgcgcaga ctgcctggtt tggcgccgtg caagcagatc
     6121 ccaagggtac aaacgcgccg ctgcgtgcgg cggcatcggg aacgaaggcc acgctggtct
     6181 ggtgcaggct ttcccggaat ccttcatagt ttgcaaaaac gaaggtcttg tctttttgaa
     6241 tggggccgcc gatggctccg ccaaactggt tacgccggaa ggggggcgct gatcccaggt
     6301 cgaaaaaatt cggctcgtcg aaaatatcgt tgcgcacaaa ttcaaagagc gacccgtgcc
     6361 actgattggt gccggaagtc gtgacgatgg tgacctgtcc accgggatgc ttgccgtatt
     6421 ccgcgccgta ggtatcgctc agcaagttga attcgcggac tgcgtccacg ccaagcattt
     6481 ggccgctggt gcctccgggg gtcatgttat tttccgccgc gcccgtatac tcaatcccgt
     6541 tcaacagaaa caagttctgc tgcggcctgt ttccggccac ggaaaacata tttgcgttgg
     6601 tcgagttgga aacgccgacg cctccggttt tttccgacgt gaatgtgacg acccccggat
     6661 tgagcgtcat gagttcgtca tagcttctgc cattcagagg aaggtctttt acttgctgtt
     6721 cgccgacaac gccggaaatg tccgcggccg ttacgctcac caccggagca tcttccataa
     6781 ccttaagctg ctcggtaact tcccccagct ttaatgcaag gtcgacactg gcttcttggc
     6841 caacgactag gtgaattccg ctgcgcacct gctcctgaaa tccttgcttt ttagcatgca
     6901 cttcatactc gccgaccgcg agcgatggta catagtagcg acccgcatcg tcagtaatgg
     6961 cgctgcgtgt ttcaccggtt tcgatatttt ttgttgaaat cgccgcgccg gtgacggttg
     7021 ctccggttgg atccgtcact ctgcccgata tcgaggcgga tacttgcgca ctcgttgccg
     7081 gggcgcacgg caggatcgcc gccagcaagc cacctgcaag gaaaaataga aatcctggaa
     7141 aagtacgagc gctgaaacga gttttggaat tggaaacgaa tagcgcggca atacgactca
     7201 tcaaactccc ccctctggaa taattaattt tcgcaacatg gaattacttt actcacccca
     7261 aagaaatcag ttagatgatt taatacaatc aaaatcgata tgtcaaatcg acttaatacg
     7321 aataggaagt atatgaattg ctatggccgg aggcggagaa tcgtatgcgt tgccgcttca
     7381 aaggagggct tctgtttttc attcgcagcc cggcgtgcga tttcatttcc tgtcagtggc
     7441 caccaaactt tcggcaagaa acaagattga aggaaaaatt gtggagcttg aagcggaggg
     7501 tgccctggga ccgtttcggc tggttgctcc tcacgaaaag cggcctgcgc gttgggtgga
     7561 aatggtcaaa tcgatcacgg ttgttcgcgt gccataggtt gaatgtggca taggcttcag
     7621 cctgttgggg gtagtccttg cagggaccaa aatccacagg ctgaagccta tgccacataa
     7681 accacaatca ttcattcctg aaaacgcttc catctttcat tacgaaggaa actttgcgca
     7741 cggcttgaat gtcgtccagc ggattttctg gcgtggcgat gatgtccgct gccagacccg
     7801 gcttgattgt gccgcgctcg ccgtcgacgc cgagcaaacg cgccgcattg atggtcatgg
     7861 cgcgcagcgt gtctttcgcg gggacgcccg cctccaccca ggagtcgacg tattccacgg
     7921 cttgtgttcc acgcgtttct cccggcacgt acacgaatga gtctgtgcca aaggccatcg
     7981 tcacgccgat ctggtaagcg cgtttcagcc ggtcaacgaa tacggcgtgc atatcaggca
     8041 tgccactgtt gcgcgcggcg ctttccgtaa aatccgtgcc aaccagcacg acgtgattct
     8101 ttttcgccag ttgtaaatct tcattggtca tcatttctcc gtgctcgatg gaatcgacac
     8161 ccgcttcagc ggcattgtga gcgccggcgc gagtccagca atgtgcagcg agtttcaatc
     8221 catcgcggtg cgcctcttcc accatgaagc ggatgtcgtc ggcgctgtaa atatatttct
     8281 ggtcgtcaac cactatcttg atcactgtcg cgccgtaatg aatgttttcg cggatggctt
     8341 tgatcatttc gtcatgagta tcggcgaaag catattccgg ggtggcgagg tcgcgctttt
     8401 ccggctgcaa atggaattgc ccgccatacg gagcgatgat gcgccccgcg ttgagcatgg
     8461 tggggccggg cacgagtcca cgttcgatag cttcgcgcaa tgcagaatca gcatagttgc
     8521 cggcattgcc tacgtcgcgc accgttgtaa agccggcagc caacatatcg cgcgcattgg
     8581 caacgccttc aatcgcgcgg taaggcgtcg agtccagcag cgtcgtgaag taataattat
     8641 taccgtcacg atcttttttg agtgtcatgc acagatgcgt gtgcgcgtcg aagaggccgg
     8701 gaagcaccgt ggattttgag agatcgatca tttttgcgtc ggcgggaatc tgtagattcg
     8761 cgccgaccgc ttcaatcttt tttccgcgca ccagaataat ctgatttgtt tccgttgcgc
     8821 ctttttcagg gtcaaccagc ttgccggcct tgatcgcgta aagcttcgcc ggccccggtt
     8881 gcgggtattg cgccaataat tttggcgatg cgccaaacac agagagtagc agcaaaagaa
     8941 atgtgcaaaa tggaagcaat cgcgaacgca tcggcagctc cttaaagaag atggaatgtt
     9001 gccagaaatc aataactctg ggaagggacg gaaggatgag aatgttttga ggcgttatgc
     9061 gcgtttaggg aatgcggctt tgacgtctac tcgcaggtac tcgtacatct ggtctgtgac
     9121 gttctcgccc atgtgctggg actgggaacc attcgcccac aaaacgtgtc cggccttgtc
     9181 gtgtgcttcc tcggttttgc cgctttccag tgtttggtga agattctggt cggtcatgaa
     9241 aatgattacg gagccgaggg gatggttgtg catttttact ttgtcgagtg cgggaatgcg
     9301 gcatcgaatc actctgacaa attcgttttc aaattccagg tggtagtgtt ccgggtcttc
     9361 gaccgtgcat tcgtgggaca tgtctacgcc tggcacgtgg ggcgcggggt ttgaattttg
     9421 cgcgccctgt aatggcagac catgagataa cgccactgct gcgagtgaag cgcctgccgc
     9481 agcctgcata agttgtcgcc ggtttaattt ctcaggcatg gtggacctcg cttcgacagg
     9541 ggatgtgggt ttatgtggca taggcttcag gactaacccc cacaggctga agcctgtgct
     9601 acttaaacca tctgccgtga tctagagcat ttggcgcagc acgtacggta ggataccgcc
     9661 gtgcctgtag tagttgactt cttctggcgt gtcggcgcgc gctaccgtct ggaacacggt
     9721 ttccttgccg ctcttgtcgc gagcacggac ttcaaccgtc attccgggag caagtttcga
     9781 aatgccttcg atcgtgaact cttcgaaccc ggtgaggcca agtgactgcg tggattcgcc
     9841 tgcgcgatat tgcagtggga gaacgcccat gccgagcaga ttgctccgat ggatgcgttc
     9901 gaaactttcc gcgatcactg cgcgcacgcc gagcagcact gtgcccttcg ctgcccagtc
     9961 acgtgaagac ccggaaccgt attccttgcc gccgagcacg atcagcggcg tgccttcctt
    10021 ctggtatttc atcgacgcgt cgtagatgga cattttttcg ccgtcgggtt ggtgtacgga
    10081 ccagccgcct tcggtgcctg gtgcaagctg gttgcgcaag cgaatgttgg caaacgttcc
    10141 gcgcatcatc acttcatgat tgccgcggcg cgcgccatac gagttaaagt ccactggctt
    10201 gacgccctgc gcgatgagcc actttccggc cggagtatcc acgccgatgg atccagctgg
    10261 cgaaatatgg tcagtggtga tgctgttgcc gagcatcgcc aatgcgcgcg cgttgtgaat
    10321 atcggtaagt ggcgcgggag tcttcgtgat tttatcgaag aaaggtggat tttttatgta
    10381 cgtggacttt tcgtcccacg cataaatatc gccttcggga actttcagct cctgccagcg
    10441 cgaatcaccc ttaaacactt cgccgtattc cttgttgtac atcgcggtcg tgacgctggc
    10501 gcgcacggtg gattcgattt cctgcggcgt tggccagata tcgcggagat aaactggctt
    10561 gcccgctttg tcatcgccga gcggttccgt ggccatatca aagtccattg tgcctgcgag
    10621 agcataggca accaccagcg gcggcgatgc cagatagttg gcccgcacga gcgggtggat
    10681 gcgtccttcg aagttacggt ttccgctgag cacggagacg gcgacgagac tgttttcctt
    10741 gatcgccgtg ccaatgtgct cttcgagcgg gccgctattg ccgatgcacg tcgtgcagcc
    10801 atagccgacg agttggaagc caagctcgtt gagatatttc ccaagacctg cagcttcgag
    10861 ataatcggta actactttcg aaccgggcgc gaggctggtc tttacccagg gcttgctaag
    10921 caggccgcgc tcgattgctt ttttcgcgag tagacccgct cccagcatca gagaaggatt
    10981 cgaagtattc gtgcaactgg tgatcgcagc gatgacgacc gagccattcg aaagctcgaa
    11041 cttgtcgcca ttttcccgca cagagacatg cttttgcgcg gcaccctcca tcgacttttc
    11101 gaacgacgct ttcgattcgc gcagcggcac gcgatcctgc ggacgcttcg gtccggccag
    11161 cgtcgcttcg acagtggcaa gatcgagttc caatttgtct gtgtacatcg gatcgggcgt
    11221 agcgtcagtg cggaagatgc cctgcgcctt ggtgtactcc tcgacgaggc gcacgcgctc
    11281 cttgctgcgg cccgtgaagg tcaaataggc aagtgtttcg gcatcgacgg ggaagaagcc
    11341 catcgtcgcg ccgtattcgg gggccatgtt ggcgatggtc gcgcggtccg gcaggctcag
    11401 gctcgaaagc ccagtgccgt agaactcgac gaattttccg accactcctt ttttgcgcag
    11461 catttcggtg acgaccagca cgaggtccgt cgcggttgtg ccctcgggca ggcgaccgtg
    11521 cagcttgaag ccgactactt cggggatcaa catcgacaac ggctggccga gcatggcggc
    11581 ttctgcttcg atgccgccga cgccccagcc gaaaacgccc aagccattga tcatcgtggt
    11641 gtgcgaatcg gtgccgacga gagaatcggg gaatgcatac gtcttgccgt ccgcagcagc
    11701 gtcaaacacg acgcgcgcga ggtattcgag gttcacctgg tggcagatac ctgtatcggg
    11761 cgggacgacc ttaaaattgt tcagagcgtc ttggccccag cggagaaagc catagcgctc
    11821 gacgttgcgc tggaattcga gttccgcgtt aaatgcgaaa gccatgtcgg agccggactt
    11881 atcaacctgc acggaatggt caatcacgag ttcagcaggc agcaacggat tcactttttt
    11941 cgggtcgccg ccgagtttga cgattgcttc gcgcatggcg gccagatcga ctaccgcagg
    12001 cacgccggta aaatcctgca agagcacgcg cgcgggcatg aaagaaattt ccttctgtgt
    12061 ggcggctttg gggtcccaga aagccagcgc ttcgatatcc gcgggatgga cgaattttcc
    12121 gtcttcctgg cgcaaaagat tttcaagcag tacgcgcagc gaaaagggaa gcttggagac
    12181 gtggccaact cctttttttt caagcgcgtc taaacgaaaa atttcgtagg gcttgttgtc
    12241 aaccttcagc gattcttgcg tactgaacga atttttagat gcgggagcca ttatggcgtt
    12301 tttcctttct gatatattgc aggacgacaa tcataacgca tcccggtgtt ccgcgcagct
    12361 caagactcct atgaaatatt tttgcttgtg tggcccctcg ctcagtctgt acattgtgag
    12421 ggtcgaggaa tggcgagggc accaatagca caactaaaca gaatcagatt ttgctggtaa
    12481 aatgctgata caccatgccc ggcgcctctc aaaaggagat attcatgaaa aagttgattc
    12541 tcgcgtttac agtgattgct tgtgcactca ccatttccac gggcaccaag gctcaggatg
    12601 ctcctaaagt acacagcatg actggttgcc tgcgggctgg tacggcgcca gatacctaca
    12661 tgctcacaga cctcgaaaag ggtccaaaga gcgtgggaat cgtttcgtcc tcggccaagc
    12721 tagcacccca tgtcggacat aagattgaaa tcacagggac agcggttcct ccggctgaag
    12781 cggaagcgat gaaggatgta ccgaaagctc cccactatat gaaggtgacg ggcgtcaaga
    12841 tgatttcaac gacctgccca taaggattta attttttcac ctcggcggtc gtccaatttt
    12901 cgcgcgactg ctcgagatgt tcattcctat ctgcctcgtc ccgaaggcac agccagcggg
    12961 gcgaggcatt tttattctgg aatcgttccc gtgccgtcag ttatggttag tatgaggatt
    13021 cgccagacgc atacccaaat gaaaattctt ctgatctcta cgtatgaact cggcaggcaa
    13081 cccttcggct tggcttcgcc tgccgcgtgg ctgcggggtg gcgggcacag cgtggtaagt
    13141 ctcgatctga cgcgggaaac actcgaagat gagcaaattc tggcggcaga attaatttgt
    13201 ttttatgtgc cgatgcacac ggcgacacgg cttgcagcgg ggcttattcc aagagttcgc
    13261 aaaataaatc cgcaggctca cctttgtttt tacggactgt acgcgccagt gaatgaagaa
    13321 tacttgcgcg ggctgggagt gggcacgatt ctcggcggcg aattcgaaga ggggttgatg
    13381 agccttgcgc agagatcgca gcagggtgca gcgaatggaa attctccggg cgcgcaggcg
    13441 gaaccggtga tttcattggc gcggcagaaa tttctggtgc cggaccgcgc gggaatgctg
    13501 gatcccgcga aatatgcgcg cgtggtgatg cccagcggag agcaccgcgt cgcgggctcg
    13561 acggaagcga cgcgcggctg caagcacctg tgccgacatt gtcctatcgt gccggtgtat
    13621 aacggcgcgt ttcgtgtggt ggagcgcgag attgtgctgg aagatattcg gcggcaagtc
    13681 gcggcgggcg cgcggcacat tacgtttggc gaccctgatt tttttaatgg gccgactcac
    13741 tcgatctcca tcgtgcgcgc gatgcaccgc gactttcccg aactttcgta tgacgtgacg
    13801 attaaggtcg agcatttgcg cttgcacgac gcgcttttgc ccgtgctgcg cgatacggga
    13861 tgcctgtttg ttacgagcgc tgtggaatcg gtggacgatg ccgtgctcga aaaatttgac
    13921 aaggggcaca cacgcgccga ttttcttgct gtcattgcgc gctttcgaga actgagcatg
    13981 acgctgattc ctactttcgt tccgttcacg ccgtggacta ctttttccgg ttatctagat
    14041 ttgctggacg tactttcgac ggtaggattg actgaaaatg ttcctcccat tcagcttgca
    14101 attcgtttgc tgattcccgc aggttcgcgg ctgctggaac tggccgaagt gaaagcaata
    14161 attgatccct tcgattcctc agcgctggtt ttcccctgga cacacgcgga cgcgcggatg
    14221 gatgcgctgg cgcgcgaaat ttcgcagatt gtgcagcgcg gtgacggctt gaagctttcg
    14281 cgtacggaaa ttttttcgca tattcgtcgc gccgctcaag ctgcatcggg gaatcgcaca
    14341 ccggtcgtcg gcgcggcttc actggcagtg aaaagcgcgc ctgtgccata cctggacgag
    14401 ccgtggtatt gttgcgcgga accgatggac gcccaatttg tttccatagg aaaaccgaag
    14461 gaagcagtcg ccaaagccga ccagtatgtg tgaggataat tccgcgaata aattaaggtc
    14521 atcaatttgt ctatgagcaa tatttatagc cgcctgctgt agcccgcctc tcttgtcctg
    14581 agtgaaaacg aaggatcaga ggcgggggtt tagattaccg ggtggcagga atgtagtgga
    14641 cgagcaagat ccgaacgttt gaagcgcgca tgcaccagca gaaaaccccg cctctgatcc
    14701 ttcgttcact caggataaga gaggcgggct acagtggccg ggtagagtat tgggacgcac
    14761 cagtggcctg aatattttgg cattcgattt cttgggaaga caaaacatat gagcagacta
    14821 ctgattcttg caagcaagtt gggttaccag acgcgcagtt tcgcggaagc cgcgcgcgca
    14881 ttgggtgttg acgtcgtgtt tgccagcgat cgctgccacc agttggaaga tccgtgggcg
    14941 gatggcgcga tcgcagttcg cttcgacgag cctgaagaat cggcgcggca aatcgtcaag
    15001 cagtccttcg tgcgccctgt ggacgggctg atcgcgctgg gagataggcc cgcggcaaca
    15061 gcggcgcatg tagcccgcgc gcttggactt tctttccatt ccccgcaagc cgtggaaaat
    15121 tgccgcagca aattgcgcca gcgggaagtt ttgcgggatg cgggactgcc cgtccctgga
    15181 tttttttcct tcaagctgga taaacgcgtg gagaaaattc tgccgcgagt tcaatttcca
    15241 tgcgtggtca agccgctgcg gctggccgca agccagggcg taattcgcgc gaataacccg
    15301 gaagaatttc aggcagcggt tgcgcgcatc aaacaattat tggaatcacc tgaggtgaag
    15361 gtcacgcgcg aaccggagct cgatcgcctg atggtggagc gctacattcc cggtagggaa
    15421 gtggccgttg agggactgct cacgcacggg aagctgcgaa ttctgggtgt ttttgacaaa
    15481 cccgatccgc tcgaagggcc atattttgag gagacaatat acgtaacgcc atctcgcttg
    15541 ccggatgaaa tgtacgatcg cgtcctggaa tgcgccgagc gcacggtgaa ggctttggga
    15601 ttgacgcatg gcccggtgca tgcggagttt cgtatcaatg atgaaggttc gtgggtgctg
    15661 gaagcggcgc cgaggcctat cggcgggctg tgttcacgcg cgcttcgatt tgggccgcag
    15721 cgcatgttgc tcgaagaatt gctggtgcgc cacgcgctgg gcatgcaggg aacggacatc
    15781 gcccgggaag aggacgcgtc gggagtgatg atgattcctg ttccgaagtc tggcgtactt
    15841 gaaaaagtgg agggcgagga gcaggcccgg gcgacggcgg ggatcgaaga tttgcaaatt
    15901 acggcgcgat cgcatgatgt gattgcggcc tggccggagg gggcttcgta tcttggattt
    15961 ttatttgcgc acgggaaaac tgcggccgag gtggagagtg cgcttcgggc tgcgcatgcg
    16021 gttttgaagt ttgagattac ggagcggctg ccggtgcagc atccggtgac tggagttgcg
    16081 ggcacggctt agcctgccct tctcacaagc agtgtgcgcg caattttgtc ttggcgcaca
    16141 ctcgctgatg atcctggtgg gttgtgcata tgctgtagcg gcatggccat gccgttcacg
    16201 gagatccttc tcggtgaacc gtgcccctac aacctcgcca ccagcaaaat gaggaatcgt
    16261 tcgtcgaaat acccggcggt ctaaaccgca tttgtgagaa attcgggtca gcgacaggct
    16321 tgttttcatt aaaagaaaaa aatgccggcg ggacgccggc gccacaaaag ctgatttcct
    16381 tcggacattg caatattgat tattttagcc ctttgctttg ggggctacgt atccttcagg
    16441 gagtttggcg ccttcgccaa agaaatattg ttccatttgc tcttccataa ttttctggga
    16501 ctgcgggtcg agagggttca gccgatattc gttcatcagc atcttggaat attcgatcca
    16561 aagcttccac gcatccgccg aaacattttc aaaaacgcgc ttgcctattt cacccttcca
    16621 cggaatacgc tccaggccgg gcatgtcctt atcgaacttt acgcaatgga ccatgcgctg
    16681 accgggcttc gccgtagtgc tcggagccgc tggttttact ggcccgcaag aatcatccgc
    16741 catctgcatc ctccaactgg aaacttggca atctctttcc tatctagggt actgcaagac
    16801 cgttagaaat ccaaaccggc tctgaccctc aattcgtttt cgaagagtgc ggtgatttct
    16861 tccccagttc gcttacaata cgagagtcac gcagtcggct ctgagggggc gcgatgtcgg
    16921 acgttacggc gatcttttgg gatgtgggcg gcgtaatcct gtccaatgga tgggaccgca
    16981 atgctcgcgc cgcggcggcc aagaaatttg gaattgactg ggaagaattg caggatcgcc
    17041 acgaccttgc cagccctgct tttgagacgg ggcggatcac cctcgatact taccttcagc
    17101 gcactgtttt ctaccgtaag cgaagtttta cgaaagaaga atttacagat tttatttttg
    17161 cgcagtcccg ggaatttcct gaatcgcgct cggtgttgtc cgacgtggcg gccaccgggg
    17221 aatatttgca ggcgaccatc aacaatgaac cacgcgaact gaatgagcgc aggattcatg
    17281 aattcagttt gcggcgcgaa ttaaaagctt ttttcagttc ctgttatctg ggcattcgta
    17341 agcccgacga aggtatttac aagattgcgt tggaagtgac gcagcgcaat cctgatgaat
    17401 gtttgtttat cgatgatcgc gaactgaatc tggaatgcgc gcggcagttg catatgcgca
    17461 ccatacattt tcaggacgcg gcacagctgc ggcgcgatct gattgcaagc ggagtcagca
    17521 ttaacgaatc gtagaggcgg aggcgaatat ggaactcgga atggttggac tggggcggat
    17581 gggcgcaaat atggtcgagc gtcttttgcg cagggggcat cgcgtggtgg gcttcgatcg
    17641 cgatgctgga gccgtggcgc gtgtaacggc ggccggagca ggaggcgctg attcgttggc
    17701 gaacctcgcc ggaaaactcg ggaaacagaa agcgatttgg atcatggtgc cttccggcgc
    17761 gccggtcgac caaaccatcg ccgagctgat tccgcatctt tctaaaaatg atattctgat
    17821 agacggcggc aacagtaatt ataaagatac gcagcgccgc ggagtaagtc tgaaagagca
    17881 gggatttcag tttgtggacg tgggaacaag cggcggaatc tggggcctga aagaaggcta
    17941 ctcgatgatg gtgggcggtg ataatgccgc gattgattat ttgcgcccaa tatttgaaac
    18001 acttgcgccg gggaaagacc tgggttgggg gcacgtcggt ccatccgggg cagggcattt
    18061 cgtaaagatg gtacacaacg gaattgagta cggaatgatg caagcgtatg ccgaaggttt
    18121 tgcaattcta aaaaagaaaa atgaatttgc actcgacatg caccaggttt cggaaatctg
    18181 gcgcactggc agcgttgtgc gttcctggct gctggatttg ctgagcattg cgctgaagga
    18241 aaacgcggaa atgaccggta tcgctcccta tgtgtcggat tcgggagaag gccgctggac
    18301 tgtggcggaa gcgatcgatc tagatgtacc cgctccggtg attactttgt ctttgctcca
    18361 gagactgact tcccgggata cggattcgta tgccgacaaa cttttggcag caatgcgcaa
    18421 tcagtttggc ggccacgcga tcaaagcagc cgcaaaaaag tgagggcccg aacatgactt
    18481 caggtaaaag cgcaacaatg gagcgcgcgg aactcccaaa acagagctcc atcactccgg
    18541 cggatccatg tacgattgta atttttggcg cttcgggcga cctatcacgc cggaaactcg
    18601 tgcccgcgct gtatggcttg gccgcacaga actgcctggc gcgaagattt gcaatcatcg
    18661 gattcgcacg aacgcccatg acggatgagg catttcagca aaccgccgtg gattccgtga
    18721 aaaaattcgc cgacgcgaac acgttgcggg aagaggaatg caaggagttt gcacatgctc
    18781 tcgcctatgt ttcaggcgaa tatcatcatc ccgaagcgtt cgagaaatta aaggctcgcc
    18841 tggacgaact cgatcgcgcc cataaactga acggcaatcg ccttttttat ctggcgacgc
    18901 ctccggaaat ctatcccacc attattgaac aattggatcg cgccggcctt tcaaggaatc
    18961 cgaacggaaa atcatggacg cgagtcatca ttgaaaagcc atacgggcgc gacctagcct
    19021 cggcgcgaaa gctgaatgaa tccgtccgca aggcgttcga tgaaacgcag gtataccgaa
    19081 tcgatcatta tttgggcaaa gatacagttc agaacctgat ggtgctgcgt tttggcaacg
    19141 gaatttttga gccgctgtgg aatagaaatt atgtggatca tgttcaaatc acggcggcgg
    19201 aaacactcgg cgtagaacag cgcgcggcat tttatgaaac tgcgggcgca acgcgcgata
    19261 tgattcagag tcacgtgctg cagctgactt cgctcgtggc tatggaaccg ccggctgctt
    19321 tcgatgccac cgccgtgcgc aacgaaaaaa ttaaagtgct gcaatccatt cggccgttta
    19381 cgcccaaaag catcaagacg gatgttgtgc gcgggcaata tggcccggga agcgcgcccg
    19441 gaaattcctc cctgcttgcc ggatatcggg aagagcctgg agtggcgaag aattcaggga
    19501 cggaaacttt tgttgccgca agtttgcaga ttgacaattg gcgctgggcc ggtgtgccgt
    19561 tttatctgcg cacaggcaaa cgactccctt cacgtgtcac ggaaattgcc atacaattca
    19621 ggaaagcacc gcacctggta tttcgaggac aagacctggc gaccaatgcc cttgtcctga
    19681 atgtgcagcc cgacgaagga atttcaattt catttcacgc caagttgccg ggacaggaaa
    19741 tgaaactgaa aacggtcacg atggatttca gctaccaggc tgaattcggc gggggagagc
    19801 gatccgcgta cgctacgctg attaacgatt gcatgcgcgg agacgcgaca ctgtttgacc
    19861 gcgccgatgg cgtggaagca gcgtgggcgt tggtggatcc gatcctgaat tattttgaag
    19921 cgaataaacc caaatttccc aactactcgg cgggtacatg gggccctcat gaagccgatg
    19981 aattactgga gcgcgatggc cgacagtggc gaaattcgta ggggagaatt gccggagaat
    20041 cctcccgaaa tacaaatttt ccccaacccg gctgatgtcg cacgggctgc ggcacgattc
    20101 tttgttgatt atgcgtggca atgcatcgcc aaggatggcc agtttatggt ggcgctgtcc
    20161 ggcggaaata cgccgcgcca gatgtttgaa attcttgcct ctgatgaatt tcgcggtcag
    20221 gtggattggg ccaaggttca tgttttttgg ggggatgagc gcgctgttcc tcccacaaat
    20281 ccggaaagca attacgggat ggcgcgacgc gaactgctga tacgcgtgcc ggttcccgaa
    20341 ggaaatgtgc atcgcatgga agcggaaaag gccagcatcg gccgcgccgc gcacgaatat
    20401 gaggaagttc tcaggaaata tctcgagttg gacgaccagg gtttcccgcg attccattta
    20461 atatttcttg gaatgggaaa tgatggccat accgcatcgc tatttccagg gccgcgcgta
    20521 acgcggcaaa cgtctcgctg ggtcagcact ccaacggtaa ctaaattgaa tatgcggcgc
    20581 atgacgttga ccttgccagt gctcgacgcg gcgctgcgag ttgtttttct tgtcgtaggt
    20641 tcggaaaagg cgcaaatgtt gcacgcggtc ttggaggaaa aacacgatcc gccgtatccc
    20701 gcgcaactcg tgcaaccgcg gcaccgtgga ttaaaaatct tcttggtgga taaagcagcc
    20761 gcggcgttgc ttacgcacag ggggtcaaaa cagccggcaa cggatgggaa gcccgccggt
    20821 gcgactcgtg gcaaaaccgg gagaaacatg tgatcctggc cggggacatc ggaggcacca
    20881 aatgcaattt ggcgctgtac gatattcatg gcgtttctcc gaaaaaaatc ttcacgcaac
    20941 gatatgaaag ccgcgatttt cggactttcg atcagttgat cgcaaagttt ctaacggact
    21001 cgcaatcgga atccaaaata gccggcgata tggcggtaga agcggcaggc tttggagtag
    21061 cgggacctat catcaatcgt cgagtcaagg ccactaacct tccgtggatc gtagatgccg
    21121 cggcccttgc gactcagctg aatacccgcc acgtgatgtt gctgaatgat ttggaagcaa
    21181 ccgggtacag tttgtcgcat ctcgcggcgt cggaaatttc tattttaaat cgcggtgcag
    21241 ccacgccgaa cttgacgcag gcgctgctcg cggcgggcac aggattgggc gagacgattt
    21301 tattttggga tggcagcaag tatgtaattt cgagttctga aggcgggcat acggattttg
    21361 cgccgcgcac ggaagtggaa atcgagctgt tgcgctatat gaaaaagcgt aatgaattcg
    21421 tgagtgtgga attaattctt tccggccgcg gttttcggac gattcacaat tttctcaacc
    21481 cttccgtcga gcatccttca tttgaagatc cgcacgcgga tgcggctccc gaaattacgc
    21541 gtcttgggat ggcagggcag tgtccggttt gcgtgcaaac attggacctt tgggtgagta
    21601 tgtacggtgc agaggcgggc aatcttgcgc tcaaggttct ggcgcgtgga ggagtttggg
    21661 ttgccggagg tatcgcggtg aaaatacaaa agaagatgga agacggaaca tttttccgcg
    21721 ccttctgtgc aaaagaaaaa tttgccgagc ttttggcgca gattccaatt tacatggtgt
    21781 tgaatgaaga ggccccgctg gttggagcgc tatcccaggc catgaaggcg gcggggatac
    21841 aattaccgta gcaattcaat ctattcattt aatcagggat ttccggtgga ttgcacgtgg
    21901 ccccgcgctt gaatttcaac agtcaccgta tcgctgacat gcacaagaaa gttgcttgga
    21961 tttttcagtc cccactgcac gtagggaata ttcatacgag tggtgatgtc gagagtcttg
    22021 ccattcacct tcacatcgag cggcagcgtt acgtcatgtt cctgtccatg caagcgaaag
    22081 cggcctgaca cttccaccct ggaaatcccg tcggcggcga caacccccgt gagttttgcg
    22141 ggcgaaaaaa caatttcagg atactttacg ctttccaata tttcgcgatg catgcgagta
    22201 tcgcgcgaat tgtttccgct ttgtccgctg gttgcgtcca ccacaattgt cccgccgatt
    22261 tgtccgctcg atggatcgta atgaaccgat ccattcttga cggcaaatgt tccgtgtacc
    22321 gtgtgcaagg tacttcccag agaatattgc actttcgatt gctccggatc aatttggacc
    22381 gacaattcct gagcgcgcgc cgtctgcacg gacaccagcg tggccatcgc ggcaagaatg
    22441 gacaaggtca gaaaaacgct aaattgcctg aaaatgttag gtttttgatt catatgcgct
    22501 aggctcgcaa aaacttccag ctgaaatcgg cgatttcagc catggcaacg gaagagagcg
    22561 gaacagtccc ggtgtgaata gtcagcattc gggcgaacaa gcttggcgag atctgaaaca
    22621 ggcggagaat tctgcgacgg atccaatcgt tttcgctcat cagcgtcata aggcgggtca
    22681 tcgtgatgcc aatcgatgca attttcttgt gtgaggattc gtaaaaggcc aagtcgcgtt
    22741 gttcgatgct ttccgcgagg gtaattgctt gctgaaacga aaggctgagc ccgtgcccgg
    22801 ttactgcgtc cactgtaccg gaggcatctc cgatgagagc gacacggccg ttcgtaacct
    22861 tgggcaaaat tctcaagctc gtcgtgtcgc ccaactcgcg cgtggtcggc tcaactccgc
    22921 aaagtttttc tgcaagaaaa gggaatcgtc taattgcttt gtcaaatcga agttggggat
    22981 ctcgcgaaat tacagcgact cccacttcgc gtcctctcat cggcgtgata aaaatctggc
    23041 agccattcgt ccaatgaact tcggccacat cggaccatgg tttcaaatta aagtgagtgc
    23101 aaaaaccaaa ccgcttcctt cgcgtagcgc gcggttcgag gccagcccat ttgcggactt
    23161 gcgaattttg accatccgca ccgatcagcc attcgtagga aaaacgttcc agaggcgttg
    23221 ttacagtgtg tttgtcaatt tgcgttacgc gcgctcccca tcgaaactct acgcctgctt
    23281 ccatggcgtg gttgacgagc agttgatgta atttcacgcg gcgcacggat atgccggtct
    23341 catccgcaaa atcagcgcgc acggaattgt tttcgtcaac gaatcgaatt cctcgaaatg
    23401 gaaaggcgac ttcagccggc aaggaaaaat cgagcgtctt cagggcggcg actccttg
//
LOCUS       DASZMB010000235        10376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2430031, whole genome shotgun sequence.
ACCESSION   DASZMB010000235 DASZMB010000000
VERSION     DASZMB010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 10376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10376
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2430031"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="VG272_11115"
     CDS             complement(<1..1184)
                     /locus_tag="VG272_11115"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HWF14277.1"
                     /translation="MKSRVSRIFLGVVVAAMPLFSVACGTSYSSGLGPTGGTQTGMVN
                     MMVSDASTEDWATIGIKILSISLIPQGGGSPVNVFTAGSSVPTINLVQLDQLGELLGT
                     LPVPAGTYTSAILTVSANQGDVTLIVAADPETGFAGTPGATIPANQIQILGASGAAGS
                     KTVPVNVNFNPPLTVVANQTTSLDVEFDLAHPAFLVGHVPPALGGQTIWAVNFNKGPV
                     YHHPIRDITRLVLRHAYGTVTGVSSDYSSVTFTRDFPVEPPTTPETAVTTAQSLTVLA
                     DATNGTIFYDVDAKTNVTIKNFSTLANSLTGKFVRVAARYQSNGSLVAVRMWASTSFN
                     SVWLSPEGHVLHVNANTDVITIENEDGAPIRLTVDNNTEFFFRTPWNAVSDSTPIGQG
                     TS"
     gene            complement(1753..2319)
                     /locus_tag="VG272_11120"
     CDS             complement(1753..2319)
                     /locus_tag="VG272_11120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HWF14278.1"
                     /translation="MAKKPTKKKATPSLTARAKKIRVLIMDVDGVLTDGHIWLLSRRD
                     GTASEIKGFSAYDGAGLKLARAAGLRTGIITGRESTAVAVRARECEIEFVYQHRATKM
                     ASFDEILQITGAEVNEVSYVGDDLPDIPLLERVGLAVAVANAAPEVKRAAHFVTSRSG
                     GDGAVREVIELIVKAQGKWKTFSRDAKA"
     gene            complement(2451..3410)
                     /locus_tag="VG272_11125"
     CDS             complement(2451..3410)
                     /locus_tag="VG272_11125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HWF14279.1"
                     /translation="MSLDSARRVLRIEAEAITELLQRLDGSFERAVELLLTCKGRVVI
                     IGMGKSGLIGRKIAATFSSTGTPSIFLHPAEAVHGDLGMLMRDDVVMAVSYGGETEEI
                     IALLETIKRLGLCMIALTGHPKSTIAAASDVVLDVSVKEEACSLNLAPTASTTVAMAM
                     GDALAVSLLERRGFSHGDFAALHPGGRLGKKLLRLENLMHSGENLPSVSVTAKMPDVI
                     YEISKKGLGMTTVVDADGHLTGILTDGDLRRLMQQRGAATIGLVVGECMNKNPQTLAP
                     GELAGSALRIMEDRHITSVVVIDAEKKPLGVVHLHDLWTLELF"
     gene            complement(3407..4246)
                     /gene="kdsA"
                     /locus_tag="VG272_11130"
     CDS             complement(3407..4246)
                     /gene="kdsA"
                     /locus_tag="VG272_11130"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HWF14280.1"
                     /translation="MQIAREISAGNVRIGGNQPLFLIAGPCVIESEAHALMMAEHLKG
                     IAAHANVPFIFKASYDKANRSSEQSYRGPGLAEGLRILQKIKESFGVPILTDIHDASQ
                     AIPVADVADVLQIPAFLSRQTDLLVAAGKTGRVINLKKGQFLSPWEMANAVEKVALTG
                     NDKIFLTERGASFGYQNLVVDMRSFPIMRKTTCPVVFDVTHSVQLPGGVGKSSGGQPE
                     FIETLARAGVAVGVDGVFLEVHDNPAQALSDGTNALPLVELPALLGKLKQIAALVRGW
                     SVQ"
     gene            complement(4355..6025)
                     /locus_tag="VG272_11135"
     CDS             complement(4355..6025)
                     /locus_tag="VG272_11135"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HWF14281.1"
                     /translation="MGPKYIFVTGGVVSSLGKGVAAASIGCLLESRGLRVTLQKCDPY
                     LNVDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFTHAKLTRENNWTTGRIYESILTR
                     ERRGDYLGKTVQVIPHVTDEIKSAFRKIAVDVDVVIVEIGGTVGDIESLPFLEAVRQM
                     RLELGPENFVHVHVTLVPYIPTAGELKTKPTQHSVKELLSIGIQPDILLCRSDRTVPQ
                     ELKKKIALFCNVPEQRVISMPDMDTIYAVPVALAEEGLDDQILKQLHLEARERDMSAW
                     LELLERIRKPKGEVRIAVVGKYVQLEDAYKSLRESLIHGGLAHKDRAIIEWVEAEDLD
                     SRETAAKRLEHADGILIPGGFGKRGIQGMIYAIEYARTHKVPFFGICLGMQCATIEYA
                     RDVVGLVGADSTEFDPQTPHRVIYKLRELLGVDEMGGTMRLGAWPCKIEKDSTAYKAY
                     GSTEISERHRHRYEFNCEYEKVLIDAGFRISGRSPDGKYVEIVEAPNHPWFLACQFHP
                     EFKSTPLVPHPLFYAFMGAAHTHQHRADGAASGKPSAISMTAKPSRAD"
     gene            complement(6272..7624)
                     /locus_tag="VG272_11140"
     CDS             complement(6272..7624)
                     /locus_tag="VG272_11140"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HWF14282.1"
                     /translation="MFKKAAALGLAVSMMAAPIFANEGDVPKGVPHLDHVFVIMMENH
                     GYQQVFNNPNEPYLNSLIANKKVNLATNYFAIGHPSLTNYLEVVGGSNFGVRSDNAPN
                     WHSTTCTPNIISGNVNADDGGGTTVPAPVPFETTTVCPISGTGTDAVTPAVDNWNEVT
                     LPTFPYLADIDGIKSVPAAPTEGKTIGDQLVAAGMRWKSYQESLPLTGADLVNYSNGT
                     SSNLTDFTSLAPLTSASVVQDYAVKHNPFAYFQSVQEGMPKDNGLQNVVGFEGPHGLF
                     ADLATGNVPALAFIAPNQCNDQHGRGNADAYCAFDPGIGTAGLTNGTQVGLNGGLILQ
                     GDVTIEQLVKAIKHSPAWHDGRSAIVIVWDENDYSGISTTPSGLFPPQNQNTVVLTVE
                     TNSDEPEGVQSSNYYNSFSLLKSMEAGFGLPCLNHACDKDTKVMSDLFGHDGDHDGKW
                     "
     gene            8160..10361
                     /locus_tag="VG272_11145"
     CDS             8160..10361
                     /locus_tag="VG272_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14283.1"
                     /translation="MLESNFRLGCGKVRWERLLFFFGLVAMFGILSSCGSASTAVTPT
                     ITMSCTPTDVTLLGTAQCTATVLNLSSTLVNWTVSGTNTGSITAGGLYTAPAANPTTT
                     TNVVTITATSQVQSTLTATQSLTLIPPTAISNVTCVDRNSVVATTVSSGNQISCTATA
                     STGALVPVNWSVSNKNHAAVTTNLGSISSQGIYQAALVPPPGQAVTITATSQALATET
                     LTTSVTVVFGNNVLSGPTYAFSTTGRLPTGAFWARTGWFSVGGGTIRGFEDTNQGGTP
                     NLVNNTPNNTFPPSPRPFTGSYSIGTDGRGTMQFCEDTDTACPQGSAATAYFNIVVVS
                     PSQAQVIEFSSPTSTSARAIGGGEMISQDQTVFTAGNANLSGTYSFDFSGISASGTEQ
                     SNVGDFVANGHGNISAGSSSSPAVVPGEIDTNISGTAPSQAAVTPTTYTVSSNGRGTM
                     ILNGVTFSFYPVSAGRAKFIEIDTPTLPATSASILSGDAYLQQTSSTCGWALSALNGL
                     SVFETSGASANGVIGDVGSFTGSSVANATSGTISTASVDENNGGTLASALGTLTGTYQ
                     LDACGRGTLSVGAHSYVFYIIASSNAVLQETTAGVTAHGFLLPSQGGPFVDTTLTGSY
                     AFRLGGTNASGPTANREDFVGQLTSAGTGTGLTGTLDLNDFGTTQAGIPITSGNYPPP
                     AAGIRETMMLQLGTNPASSRNFVFYMVNPTLFYVLDMDTTGTALGVINNQF"
BASE COUNT         2357 a         2870 c         2727 g         2422 t
ORIGIN      
        1 aaactggttc cctgcccaat tggcgtggag tccgaaaccg cattccacgg tgtgcggaaa
       61 aagaattctg tgttgttatc gaccgtcagg cgaataggtg cgccatcttc gttttcaatc
      121 gtaatcacgt ccgtatttgc gttgacgtgc aggacatggc cctcagggct gagccatacg
      181 ctgttaaacg atgtagaggc ccacatgcga actgcgacaa gcgacccgtt ggattgatag
      241 cgcgccgcta ctcgtacgaa ttttccagtc aggctgttag ccagcgtgga aaaattctta
      301 atcgttacgt tcgtcttggc atcgacgtca taaaaaatcg tgccattcgt tgcatccgcc
      361 aaaactgtta acgattgcgc tgtagtcacg gctgtttcag gagtcgtggg cggttcgacc
      421 ggaaaatctc tcgtgaaagt cacagagctg taatcggagg agacgccggt cacagtccca
      481 taagcatggc gaagaaccag ccgcgtgatg tccctgattg gatgatggta caccgggcct
      541 ttgttaaaat tcacagccca tatggtttgt ccgccgaggg ctggaggaac gtggcccacc
      601 aagaaagcgg gatgtgcgag atcgaattcg acgtccaggg aagttgtttg gtttgcgaca
      661 accgttagag gtggattgaa gtttacgttt actgggacag ttttgcttcc cgcggcaccg
      721 cttgcgccca gaatctgaat ctgattggca ggaatcgttg cgccgggtgt gccagcaaat
      781 cctgtttccg gatcagcggc aacaattaaa gtgacgtcac cctgattcgc gctgacggta
      841 agaatcgcgg acgtgtaggt gccagccgga acaggcaggg ttcccaataa ttcgccaagc
      901 tgatcgagct gaaccaaatt gatggtgggt acggaacttc cggcagtgaa aacgtttaca
      961 gggctgccgc caccttgcgg aatgagcgag atgctaagaa ttttaattcc gatcgtggcc
     1021 cagtcttccg tcgaggcatc gctcaccatc atgtttacca ttccagtttg cgtgccacct
     1081 gttggcccca gaccggagct atagctagtc ccacatgcaa ctgagaacaa cggcatcgcc
     1141 gctaccacta cccctaaaaa tattcgggac actcgggatt tcatttttgt ctcctgcgaa
     1201 gactcgatgg tctccactcg tttgtttgga tcgcggccgc acgcatgtgt cgggcccccg
     1261 aagaccaatc acaaattcca gccgcaccat tacaaacaca gaagatcgaa tagagttgcg
     1321 cggaattgaa aatgattttg tagtacgaag gtaccaggaa aatgttttcg ccttgcgtgg
     1381 cgcagagtct gtgtgacaac tacagaatct ttgtgggtcg tggcttcagc cacgacataa
     1441 gccctgcaaa atgagtgcgg cttcacaggc tgcggaaaaa gtagcttatt ctgtcattcc
     1501 gagcgaagcg aggaatctct cttcgaatta agtataagaa aataaagcag gagggattcc
     1561 tcgcttggtt cggaatgaca aaatgttgag tttttccgcg gcctgttcag ccgctgaagt
     1621 ttaactttgc agttctcaca cagactcttc agcctgcgcg ggttagtcct tgcaggacat
     1681 acccagccca ggctgaagcc tgcgctacta aaaaaatctc aagtattttg cagaggataa
     1741 atattacgga gtttaagctt tagcatcgcg ggaaaacgtt ttccacttgc cctgcgcttt
     1801 cacaatcaat tcaatcactt cgcgcacagc gccatcgccg cccgaacgtg aagttacaaa
     1861 gtgcgcagcc cgcttcactt ccggcgctgc attcgcgacg gcaaccgcaa ggccaactcg
     1921 ctccagcaaa ggaatatcgg ggagatcgtc gccaacgtag gaaacttcgt tcacttcggc
     1981 tcccgtgatt tgtaatattt cgtcaaagga ggccatcttg gtagcgcggt gctgatacac
     2041 gaattcaatt tcacattccc tggcacgcac cgcgactgcc gtagattccc gtcccgtaat
     2101 aattccggta cgaagtcccg cggcgcgcgc gagtttcaat cccgcaccgt cataggcgct
     2161 gaatccttta atttcgctgg ccgtgccgtc gcggcgagag agcagccaga tgtggccgtc
     2221 ggtcagcacg ccatccacat ccataatcaa cacgcgaatt tttttcgcgc gcgcggtaag
     2281 cgatggcgtg gcttttttct tcgtcggttt tttagccatg aaagcctaat ctctccgtgc
     2341 ccccttttct ctgcgtcctc tgtgttaaag ccttccactg ctaaggcaaa agcttgaaca
     2401 cagaggacgc agagaaaaga ggagaaaacc aacgagacat ctgagaacat ctaaaaaagt
     2461 tccagcgtcc acaggtcatg cagatgcacg acgccgagcg gtttcttttc cgcatcaatc
     2521 accacaaccg aagtgatgtg acggtcttcc ataatgcgga gagcgctgcc ggcgagttcg
     2581 ccgggcgcaa gagtctgcgg attcttgttc atgcattcgc caactaccag accgatcgtg
     2641 gctgctccgc gctgttgcat cagccttcgc agatcgccat ccgtgaggat tcccgtgaga
     2701 tgcccgtcag catccacgac cgtagtcatg cccaaacctt tcttgctgat ttcataaatc
     2761 acgtccggca tttttgcggt aaccgaaacg cttggcaggt tttctccgga atgcatcaga
     2821 ttctcgaggc gaagaagctt tttgcccagc cgtccaccag ggtgcagcgc ggcaaaatct
     2881 ccgtggctaa agcctcgccg ctcaagaagt gaaacggcaa gcgcatcgcc catggccatc
     2941 gccacggttg tagatgcagt cggagccaga ttcagcgagc acgcttcttc cttcacactg
     3001 acgtcgagca ccacatcact tgccgccgca atcgtggatt tcggatgacc tgtcagcgcg
     3061 atcatgcaca ggccgaggcg cttaatcgtt tcgagcagcg cgataatttc ttcggtttcg
     3121 ccgccgtagc ttaccgccat cacgacatca tcgcgcatga gcatgccaag atcgccatga
     3181 accgcctcag cgggatgcaa aaaaatcgat ggagtgcccg tgctcgaaaa cgtcgcggca
     3241 atttttcgtc cgatcaatcc tgatttgccc atgccgataa tgacgacacg gcctttgcag
     3301 gtcagcagca attccacggc acgttcgaag cttccatcga gcctctgcaa aagctcggtg
     3361 atagcttccg cttcgatccg caaaacacgg cgcgctgaat cgaggctcac tgcacgctcc
     3421 agccgcgaac cagcgcggca atctgtttga gtttcccgag cagtgcaggc aattccacaa
     3481 gcggcaacgc gttcgtgccg tccgaaagcg cttgtgcggg attgtcatgt acttccagaa
     3541 aaaccccgtc cacgccaacc gcaacacccg ctcgcgcgag cgtttcgata aattccggct
     3601 gccctccgct ggattttcca acgcctcccg ggagctgcac ggagtgcgtc acgtcgaata
     3661 cgacgggaca cgtagtcttt cgcataatag gaaacgagcg catatcgacg acaagatttt
     3721 ggtagccaaa cgacgcgccg cgttccgtca ggaaaatttt atcgttaccc gttagcgcca
     3781 ctttctcaac ggcattggcc atttcccacg gcgaaagaaa ttgccctttt tttaaattga
     3841 tgacgcgtcc cgtttttccc gcggccacga gtaaatccgt ttgccgcgaa agaaaggcgg
     3901 gaatttgcaa tacgtccgcc acatcggcaa ccggaatcgc ttgggacgcg tcatgaatgt
     3961 ccgtcagtat cggaacgccg aagctctctt taattttttg caggatgcgc aaaccttctg
     4021 caagcccagg cccgcgatac gattgctcgg acgatcgatt ggccttgtca taggaagctt
     4081 taaagataaa cggcacattc gcgtgcgctg caattccttt cagatgctcg gccatcatca
     4141 aggcgtgcgc ttcgctttca attacacatg gtccggcaat caggaacaac ggctggttcc
     4201 cgccgatgcg aacatttcct gccgaaattt cacgagcaat ttgcatttaa aattccctcg
     4261 gaaaattgag cgattcatag cctatgcgcg accggcaagt tttgcaacgg cccgtatggg
     4321 aatcattccc aattttaaat tgtgggccaa attattaatc tgctcgcgaa ggttttgcgg
     4381 tcatggatat tgccgaaggt ttgccggacg ccgcgccatc ggcgcgatgc tggtgggtgt
     4441 gcgccgctcc catgaacgcg tagaacagcg ggtgcggcac gagcggagtc gatttaaatt
     4501 cgggatgaaa ctggcacgcg aggaaccacg gatggttcgg cgcttcgaca atttccacat
     4561 atttgccgtc aggcgagcgg ccagagatgc gaaatcccgc gtcgatcaaa actttttcgt
     4621 actcgcaatt aaattcgtag cggtggcgat gacgttcgct gatttccgtg cttccatagg
     4681 ccttgtaggc ggtggaatct ttttcaatct tgcacggcca agcgccgagc cgcatggtgc
     4741 cgcccatttc gtccacgccc aaaagttcgc gcaacttgta aatcacgcga tgcggcgtct
     4801 gcggatcaaa ctccgtgctg tccgctccta caagccctac tacgtcgcgc gcgtattcaa
     4861 tcgtagcgca ctgcatgccg aggcaaatgc cgaagaacgg aaccttgtgc gtgcgcgcgt
     4921 attcaatcgc gtagatcatt ccctgaatgc ctcgcttgcc aaagccgcca ggaataagaa
     4981 tgccgtccgc atgctccaat cgcttcgccg ccgtttcgcg cgaatcgaga tcttcggctt
     5041 ccacccactc gatgattgcg cgatccttgt gcgcgagccc gccgtggatc agcgattcgc
     5101 gcaaactctt atacgcgtct tcgagctgca cgtacttacc cactacggcg atgcgcacct
     5161 cgcccttagg cttacgaatg cgctccagca actcgagcca cgcgctcatg tcccgttccc
     5221 gcgcctcgag atgcagctgt tttaatatct gatcatcgag accttcctcc gcgagcgcta
     5281 cgggcacggc gtagatcgtg tccatgtccg gcatggaaat tacgcgctgt tcaggcacgt
     5341 tgcagaagag cgcaattttc tttttcaact cctgcggcac ggtgcggtcg ctgcggcaca
     5401 gcaaaatgtc aggttgaatt ccgatggaaa gaagttcttt caccgaatgc tgcgttggct
     5461 tggtttttaa ttccccagct gttggaatgt acggaacgag tgtgacgtgc acgtgcacaa
     5521 aattttcggg cccgagttcc aggcgcatct gccgaaccgc ttcgaggaac ggaagcgatt
     5581 caatgtctcc gaccgtgcca ccgatttcga cgatcactac atcgacgtcc accgcaattt
     5641 tgcggaaggc ggatttaatt tcgtccgtga catgcggaat cacctgcacc gttttgccga
     5701 gatagtcccc gcgccgctcg cgcgtcagga tcgactcgta gattctgccg gtggtccagt
     5761 tattttcgcg cgtgagtttg gcgtgcgtga atcgctcata gtggccgagg tcgagatcgg
     5821 tttccgcgcc gtcgtccgtc acgtacactt cgccgtgctg atacgggctc atcgtgcccg
     5881 gatcgacgtt cagatatgga tcgcattttt ggagggtaac ccggagacca cgactctcaa
     5941 gcaggcagcc aatcgacgcc gctgcaacac ccttgcccag ggaggataca acaccaccgg
     6001 taacaaaaat atacttcggc cccatccgct cagttccagt cgcctcaatt agaaaaatgc
     6061 aagacgcaga actgggtaaa acctccacta agatgttggc gtacaacgca ttctaggggg
     6121 aaaggcacac tactgtcaac gtgcagcaga aagaccctat ggatgcgggg aaaataagtt
     6181 tgggaaatcc gtcacgatgc accagaaaaa atcgccgaag attgcgcgaa atggtcagcc
     6241 atttcgcgca atcgacccag cggttttcac tttaccactt accgtcgtga tcgccatcat
     6301 gcccgaacaa atcggacatg accttggtgt ctttatcgca ggcgtgattc aagcaaggaa
     6361 gcccgaagcc cgcttccatg gacttgagca gcgaaaagct gttgtaataa ttgctgctct
     6421 gaacgccttc cggctcgtcg ctgttcgttt ccacggtcag cacaaccgtg ttctgattct
     6481 gcggcgggaa caagccactc ggcgtcgtgc tgatgccgct gtaatcattc tcatcccaca
     6541 cgattacgat tgctgaacgg ccgtcgtgcc atgccggtga gtgcttaatg gccttcacca
     6601 gttgctcgat ggtgacatcc ccttgcagaa tcaagccgcc attcaaacca acttgcgtgc
     6661 catttgtgag accggcggtg ccgattccag gatcgaatgc gcaataggcg tcagcgttgc
     6721 cgcggccatg ctgatcattg cattggttgg gcgcgataaa agcaagcgct ggaacattgc
     6781 ctgtagcaag gtccgcaaac aagccgtgcg ggccttcaaa gccgaccacg ttttgcaaac
     6841 cgttgtcctt tggcatccct tcctgcacgc tctggaaata ggcgaaggga ttgtgcttca
     6901 cggcataatc ctgcacgacg cttgcggagg tcagcggtgc aagcgaagtg aaatcggtca
     6961 gattgctgga agtcccgttg ctatagttga ccaggtccgc tccggtaaga ggcaggcttt
     7021 cctgatagct cttccagcgc atcccagccg cgacaagctg atcgccaatc gttttgcctt
     7081 ccgtcggcgc cgcgggaacg gatttaattc cgtcgatatc ggcaaggtac gggaacgtcg
     7141 gaagcgtaac ttcattccag ttgtcgaccg caggtgttac agcgtcggtg ccggttccgg
     7201 agatggggca taccgtagtg gtttcgaacg gaacgggtgc cgggacagtc gtgccaccac
     7261 cgtcatccgc attcacattt ccgctgatga tgttgggcgt gcacgtggtg ctatgccaat
     7321 tgggcgcatt gtcactgcga actccgaaat tcgatccgcc taccacttcc agataattgg
     7381 tgaggctggg gtggccgatc gcgaaatagt tggtcgcaag atttactttt ttgttggcga
     7441 tcaagctatt caagtaaggc tcattcggat tgttgaaaac ttgctggtac ccgtggttct
     7501 ccatcatgat gacaaacaca tgatccaaat gggggacgcc cttcggaacg tcgccttcat
     7561 tggcaaaaat gggggcagcc atcatagaga ctgcgaggcc cagggctgcg gctttcttaa
     7621 acatatgatt cacctttcct ttgcgggaag ctgggattgc cgccgctctg aggacgcaga
     7681 gggctggcgc gaccagcggt actctggtgt ccgcctgttg ccacgcagtt acagcaatgc
     7741 gaatttttgg tgaacgcggg cgcgaaagga aaagcgatgc cacattttta aatttattgg
     7801 cggagcgttt gaaggtattc aagcaagctt caatagtcta gaatcgttcc ttcgttttgc
     7861 ggttttggct tacggtcaat cgcagcgaaa tgcttgccgc cattcaggat aaatttggat
     7921 ttctcttgac tttatcgggg ggcgacggtc tattgtcaca ttccgctttt gtgcaggcga
     7981 cactacgatg cgacactgca tctatgcctc gcggggaatg agtttctgtc cagtttttta
     8041 aatattgcgc gacctgaagg ttgcaagtgt gtggatgcgg gtgcatttga atcccgccgg
     8101 tatcggggag ccgtatcttc ctaaagtaaa tcagagtgaa gcgggcgggg ggatttctag
     8161 tgcttgagtc aaattttcgg ttggggtgtg gcaaggtgcg ctgggaacgt ttgcttttct
     8221 ttttcggact agttgcgatg ttcggaattt taagttcttg cggctcggct tcaacggccg
     8281 taactccgac catcaccatg tcctgcactc ccaccgatgt cacgttgctg ggcacagccc
     8341 aatgcacggc caccgtcttg aacctcagca gcacactggt gaactggacc gtcagcggaa
     8401 ccaataccgg ctcgatcacg gctggcgggc tgtatacggc gccggcagcc aaccccacca
     8461 ccacaaccaa cgtcgtcacg attacagcaa cttcgcaagt gcaatccact ctgacggcga
     8521 cacaaagcct gacgcttatc ccgcccacgg ccatttcgaa tgtaacttgt gtggatagaa
     8581 attcggttgt agcaacaaca gtttcatccg gtaatcaaat atcgtgtacc gccacagctt
     8641 ccaccggcgc gctcgtaccc gtcaattgga gtgtctcgaa taagaaccat gcagctgtaa
     8701 ccacaaacct cggaagcatt tcgtcgcaag gaatctacca ggcagcgttg gttcctcccc
     8761 cagggcaagc ggttacgatt accgccacct cccaggctct tgcaactgag acgctgacca
     8821 caagcgtcac cgtcgttttc ggaaacaatg ttctaagtgg cccaacctac gctttttcca
     8881 caactggaag gctgccgaca ggggcgtttt gggcccgtac gggatggttt tcggtaggtg
     8941 gaggtacgat tagaggtttc gaggatacaa atcagggcgg tactcccaat ttggtgaaca
     9001 acacgcccaa taataccttc cccccttccc cacgtccttt caccggatcc tattcgatcg
     9061 ggaccgatgg ccgtggaaca atgcaatttt gtgaggatac agatacggct tgcccgcagg
     9121 gatctgcggc aaccgcctat ttcaatatcg ttgttgtatc tccgagccaa gcccaggtga
     9181 ttgaattcag ctccccaacc tcgacttccg caagagccat tggtggaggc gagatgattt
     9241 cccaagatca aacggtcttc acggccggta acgcaaactt gtccggcacg tattcctttg
     9301 atttctctgg aatatctgca agcgggaccg aacaatcgaa cgtcggagat tttgtagcca
     9361 acgggcacgg taacatcagc gccggaagtt caagttcgcc ggcggtagtc ccaggcgaaa
     9421 tcgacacaaa tatttctggc acagctccgt cgcaggctgc ggttactcct accacgtata
     9481 ctgtcagttc gaatggcaga ggaacgatga tcttaaacgg agtaacattc agcttctatc
     9541 ccgtttccgc tggccgcgcg aaattcattg aaattgatac gcctactctt ccggcaactt
     9601 ccgcttcgat attgtcgggc gacgcttact tgcagcaaac cagttcgact tgcggatggg
     9661 cattgagcgc gctgaacgga ttgtctgtgt tcgaaacttc gggtgcttct gcgaacggtg
     9721 tgatcggcga cgtgggcagc ttcacgggat cgagcgtcgc taacgcaacg tcgggaacga
     9781 tcagcactgc atctgtcgac gaaaataatg gtggcacact ggcatcagcg ctcgggacgc
     9841 tcactggcac ctaccagttg gatgcttgcg gcaggggcac cttgtcggtt ggcgctcact
     9901 cgtatgtgtt ctacatcatc gcatcgtcaa acgccgtgtt gcaggagact actgccgggg
     9961 tcacggctca cggcttcctc cttccctccc agggtggtcc atttgtggac accacactta
    10021 caggcagcta cgcgttccgg ttgggtggca caaatgcttc cggcccgact gccaatcgag
    10081 aagatttcgt gggacagctg acgagcgcag gaacaggaac aggcttgacc ggcacactgg
    10141 atctgaacga tttcgggacg acgcaggcag gaatcccgat caccagtgga aactatccgc
    10201 cacctgcggc aggcatccgc gaaacaatga tgcttcaact tggaacgaat ccggcttcgt
    10261 cacgaaactt tgtattctac atggtgaacc cgacactgtt ttatgttttg gacatggaca
    10321 caacaggcac agcgctcggg gtcatcaata accagtttta acggcattaa gaactt
//
LOCUS       DASZMB010000236        11144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1367914, whole genome shotgun sequence.
ACCESSION   DASZMB010000236 DASZMB010000000
VERSION     DASZMB010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 11144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11144
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367914"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="VG272_11150"
     CDS             <1..1029
                     /locus_tag="VG272_11150"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWF14284.1"
                     /translation="TTDVLLHPSFPADEVSQYQQRAKSALMQQRSQPGFLASQTMSRV
                     LYGTYPAAVVSSTPESLDSLTPAMLAEWHGKHYAPQNTILAISGDVHAEILIPKLRQW
                     LSEWQRTKEIVKFPPGPGPAQKEKIFLVDRPGSVQTTLLMGNLAIDRNNPDYPALVVL
                     NEVLGAGSASRLFLNLREEKGYTYGVYSSVTARKYAGPWTAGGDLRTEVTDGAMTEFL
                     RELNRIRDEKVPEAELDAARRSVVARFALSLESSQQLIGYAITRKAYGFPADYWDKYP
                     AQIAAIGAEDVQRVARKYIDPGTMQVVAVGDASKIKTVLDKYGPVEIVAPSANPAEAT
                     GKAAGAGL"
     gene            complement(1109..1909)
                     /locus_tag="VG272_11155"
     CDS             complement(1109..1909)
                     /locus_tag="VG272_11155"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HWF14285.1"
                     /translation="MYPFIHIGPLTLGTYGLMVAIGLICAFYLLRLDFTRRGISADAE
                     AIIGITGLAGLVGSRLYHLLESPAEFFADPLPQLFSTMGFAFVGAIIGGFIALVLLAK
                     KFRMSPLLMLDAASPAAAIGYGIGRIGCLISGDGDYGRPTNLPWGMAFPHGIVPTTES
                     CVEWGAPANCRVHPTPIYEFLVAILIFYILWRLGERVINKRAPNGIIFAAYLVLTGVA
                     RFLVEIIRINPRSFFGMTNAQAASVVSVVAGIALFFYCRKAAPRNVRA"
     gene            complement(2215..3804)
                     /locus_tag="VG272_11160"
     CDS             complement(2215..3804)
                     /locus_tag="VG272_11160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HWF14286.1"
                     /translation="MTEPFEAPWKPKYNPWLIAMTVTLATFMEVLDTSIANVSLPHIA
                     GSLSASQEESAWVLTSYLVSNAIILPISAWLSTIIGRKRFYMSCVALFTMSSFLCGLA
                     PSLGVLIFFRILQGVGGGGLQPSEQAILADTFSPAKRGMAFAVYAMAVVLAPAIGPTL
                     GGYITDNYNWRWIFYINVPIGLLSLFLTNRLVEDPPFLKREQERRRGIRADYVGLGLI
                     TLAIASLQIALDKGQESDWFSSRWITSLVVTAGYAFLVWIVWEWYHPNPVVDIRLFKR
                     RNFATAMFFSFTVGIVLYGTTFMIPQFLQVQLGYPALVAGEALAGGGFIMIMVLPFVG
                     TMISRIDPRKLMAFGFVSISAAMYYMSTLFSLTMDFRAAFLMRTLQMFGLAFVFVPQN
                     VLAYVGIPREKNNQISSMNSFMRNVGGSIGIALISTSISRISQTRRTYMVANTTPGTP
                     QYESLVGGLSQNLQNQGAGSVQAAQQAHGIVSYMIDRQATTLAYVEVISILAVIILCL
                     VPFLLIMRRNKPGPGETPPAH"
     gene            complement(3928..5166)
                     /locus_tag="VG272_11165"
     CDS             complement(3928..5166)
                     /locus_tag="VG272_11165"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HWF14287.1"
                     /translation="MAVDQLPETREARTVEPVDTGSSVEGRRKTFLFAAIIGAVILVA
                     ALGFWLYARTYETTDDAQVDGHLNGVTARIDGVVKAVHVEENQSVKAGDVLVELDPRD
                     YEVAVEQAQAQVMKAEADERAENPNLPITQSTSQTSVSTSKSEVATAEAAAAAAEGEQ
                     LAAQSRLLESEANNGKAQADVARYKALVDKNEVSRSDYDQVVANANALAASVDSARAS
                     ALAAQKVVEQRRSQLEQARSLMASAAVNAPNQVAISRATLQSKHANVEAMKAQLDRAQ
                     LDLSYCKIVAPVSGVVSKRTAEVGEHVSKGERLVTVSDLGNLWVTANFKESQLREMHP
                     GQAVKISVDAFDQDFDGTVEAMPGSTGSITSLLPPENATGNYVKVVQRLPVRIRFKEG
                     QQGLDRLRPGMSVVPKVWLK"
     gene            complement(5169..6569)
                     /locus_tag="VG272_11170"
     CDS             complement(5169..6569)
                     /locus_tag="VG272_11170"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HWF14288.1"
                     /translation="MRNFRFYLFLLLMGVFAAATCAVAQTGPYQGSASAGPPSDQPVS
                     LSLDQALKMGLRYNLGGITAAQASERATGEKIVVRSALFPDFSGGVRENVQQIDLASY
                     GFKFKFPPSLGLNFPNIVGPFNFFDLRGYLTQRIADVQSIRTYQSAREAQRAAELSMA
                     DSREIVVFVVTQAYVQVLAESARVESVKAQVDSAQAIFQQASDRFNAGLSAKIDMTRS
                     NVELQTQQQRLTSEQANYAKQKIGLARLIGMSPAQVYSLADKLPYAPLDNLTVEQALT
                     LAAQNRSDLKAAEAQLHSAEFARKAAVAEHYPTLGVTADYGVIGINPANSHGTFDVSA
                     QLMFPIWAGGHTHGDIVEADANLTQRRAEYESVRSRVEADVRNAYLDLNAASEQVRVA
                     QSRRELARDELAQSTDRFASGVADTVEVTQAQEVVASAESDYIEDLNAHNLAKASVAR
                     ATGQAEKIMRDLLRSQ"
     gene            6753..7412
                     /locus_tag="VG272_11175"
     CDS             6753..7412
                     /locus_tag="VG272_11175"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWF14289.1"
                     /translation="MSARGKRGRTRPTEDARTRMCQEERTRATRRKLLDAAKRIFAQD
                     GFEAARLEDIAARAGYTRGAFYANFESKEDIFFALFEEWVRERIESVTTALRCHPDPA
                     EKLAALRTHYAKLATDRRLVLISMEFKLFALRHPEAHARLRSRHRRIRATFGELFSEL
                     MRELDTTISIAYPASSACLGAVAQGLLLEHLIDPKTLSDGDVRHALGQFFDSIFQAVP
                     A"
     gene            7726..10923
                     /locus_tag="VG272_11180"
     CDS             7726..10923
                     /locus_tag="VG272_11180"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWF14290.1"
                     /translation="MWIVRLALRRPYTFVVMSLLILILGTLSVFTTPVDIFPNIDIPI
                     IAVVYSYGGLSAEDVSQRLVVNFERGLTATVNDVEHIESQSLNGLGVIKIFFYPNVRV
                     DMAMAQVTSNAQSALRGDPPGTTPPFLLAYNASSVPILQLAISGKGLSEQQLNDMGSN
                     FLRTQLATVQGAVFPNPYGGKQREIMVDLNIPALQAKGLSPDDVVTAIGNQNLILPAG
                     TVKIGTKEDDVDLNGSPQTVNEMNDLPIKTSGSNTIYVRDVAHVRDGNPPQTNMVLVD
                     GQKAALMTILKLGSTSTLDIIKQIKERLPVVLAGLPPEFKARPIADQSLFVRASINGV
                     VREAVIAACLTGLMILIFLGSWRSTLVIAVSIPLSILTSIIVLGAIGQTINIMTLGGL
                     ALAVGILVDDATVEIENINRNLAEGKETIQAILDGAQQIAVPAFVSTLSICIVFVPMF
                     FLTGVARYLFVPLAEAVVFAMLASYLLSRTLVPTMARYLLKAHAEQSAEASLASKNPL
                     VHLQGLFELQFEKLRNSYRELLRSCLALRVAFPLIFLAICVGSFVLYPWLGQDFFPSV
                     DAGQIKLHLRAPTATRIEDTAELCSEVERAIHEQIPANEIESIIDNIGLPYSAYNYVY
                     TTSAPIGPEDADIMISLNENHHPTEQYIHDLRQKLNKSFPGVTFYFPPADIVSQILNF
                     GLPAPIDIQIMGRDVEANHEFANNLLKQLNTVTGIADLRIQQPFNQPKLHINVDRTKA
                     LQAGFTQRDVAGNLLVSLSGSFQTSPSFWLDPKTGVSYIVATEAPQYRIQSLQDLENI
                     PVTGGGGGGGTGRPPWQQLANLGTVTRGEGMATVSHYDAQTMIDIFGATQGRDLGGVA
                     KDIGKIVDANRSKLPRGSQMFVRGQIDTMRSSFTGLIGGLGFAILLIYLLIVVNFQSW
                     LDPFIIISALPAALAGIVWMLFITHTTISVPALTGSIMCMGVATANSILVVSFSKERV
                     QEGLTPQQAAEEAGFTRFRPVIMTAMAMIIGMLPMAVGLGEGGEQNAPLGRAVIGGLI
                     FATVATLLFVPSVYSLIHGFRREPGQSAEIEIPGE"
BASE COUNT         2539 a         3101 c         3044 g         2460 t
ORIGIN      
        1 accacggatg ttttgcttca tccaagtttt cccgccgatg aagtgtctca gtaccagcag
       61 cgcgcaaaat ccgcgctgat gcagcagcgc tcgcagcctg gatttttagc cagtcaaacc
      121 atgagccgcg tgttgtacgg aacgtatcct gccgcggtcg tatcttcaac gccggaatct
      181 ctcgattcgc tgacacctgc gatgcttgcg gaatggcatg gcaagcatta cgcaccgcaa
      241 aacacgatcc tcgccatttc gggcgatgtg catgcggaga ttctcattcc caagctgcgg
      301 cagtggctgt ccgaatggca gcggacgaaa gagattgtga aatttcctcc tgggccgggt
      361 cccgcgcaaa aagaaaaaat atttctagtc gaccggccgg gatcggtcca aacgactctg
      421 ctcatgggca atctggcaat cgatcgcaac aatcccgatt accctgcctt ggtagttttg
      481 aatgaagttc tgggcgcggg ttccgcaagc cgcctgttcc taaatttacg cgaagaaaaa
      541 gggtacacgt atggcgtgta cagcagtgtg acggcccgaa aatatgctgg gccctggaca
      601 gcgggcgggg acctgcgcac ggaagtgacc gacggcgcga tgacggaatt tctgcgcgag
      661 ttgaatcgta ttcgcgacga gaaagtacct gaagccgagc tcgacgcggc gcgccgctcg
      721 gtcgtcgcgc gattcgcgct ttccctggaa tcctcacagc agttgattgg ctatgcgatt
      781 acccgcaagg cgtacggatt tcctgccgat tactgggaca agtatccagc gcaaatagcg
      841 gcgatcggcg cggaagacgt tcagcgcgtg gcccgaaaat atatcgaccc gggaaccatg
      901 caagtcgttg cggtgggaga cgcgagcaaa atcaaaaccg ttctcgataa atatgggccg
      961 gttgaaatcg tcgccccgtc cgcaaatccc gcagaggcta cgggcaaggc cgcgggcgca
     1021 ggcctgtaat tttcaattgt aattatgcgg gaagctgctc gctaaagaag tgaagctaca
     1081 gggacggtat gcgagcggca caactccatc aagcgcggac attccgcggt gccgctttgc
     1141 ggcagtaaaa aaatagtgcg atgcccgcga caacggaaac cacactcgcg gcttgcgcgt
     1201 tggtcattcc aaaaaatgag cgcggattga tgcgaatgat ttccaccaaa aaccgcgcaa
     1261 cccccgtaag cacaagatac gccgcgaaaa taattccgtt cggcgcgcgc ttgttgatta
     1321 cgcgctcgcc cagccgccaa agaatataga aaattaaaat ggccaccaga aattcgtaaa
     1381 tcggcgtcgg atgtacgcgg caatttgcgg gcgcgcccca ctccacacac gattcagtgg
     1441 tcgggacaat tccatgcgga aatgccatgc cccagggaag attcgttggt cgcccgtaat
     1501 cgccatcgcc cgaaatcagg catccaatgc gcccaatccc atatccaatt gccgcggcag
     1561 gcgaagcggc atccagcatg agcaacgggg acattcggaa cttctttgcc aacaggacca
     1621 gcgcgataaa tccgccgatg atcgccccga caaacgcaaa acccatcgtg ctaaaaagtt
     1681 gtggcagtgg atcagcgaaa aattccgcag ggctttccag caaatgataa agcctggaac
     1741 cgacaaggcc ggcaagcccc gtgattccga taatcgcctc tgcgtcagcg gaaattccgc
     1801 gtctcgtgaa atcaagacgc agaagataaa aagcgcaaat caggccaatg gccaccatca
     1861 ggccgtaggt gcccagtgtt aatgggccga tgtggataaa tggatacaac catgctccca
     1921 tcttcgcgca cgtaagttat tttaggccga ttactgcgac agtatagctg agaccgcacc
     1981 gttccggtgt actggcttat gtgacataga cttcagaggc tgcggaaaaa ctcaacattt
     2041 tgtcattccg agcgaagcga ggaatccctc cttctttatt ttcttatact taaatcgagg
     2101 agagattcct cgcttcgctc ggaatgacag aataaactac tttttccgca aactgtaaag
     2161 cctatgccac atgaactcat gccggttcac ttcgtcaatt cgaatggaaa acttctagtg
     2221 tgccggtggc gtttccccgg ggcctggctt gtttcttcgc atgatcagca gaaatggcac
     2281 aaggcaaagg atgatcacag caagtatgga aatgacttcg acgtaagcaa gtgtggtggc
     2341 ctgcctgtca atcatgtatg acacaattcc atgggcctgc tgcgcggcct gcacgcttcc
     2401 cgcgccttgg ttttgcaggt tctgggacaa gccgccgacg aggctctcat actgcggcgt
     2461 gcccggcgtc gtattcgcga ccatgtaagt gcgccgagtc tgcgagatgc gcgaaatgga
     2521 agtggagatg agcgcgatgc cgatacttcc gcccacgttg cgcatgaaac tgttcatgga
     2581 agaaatctgg ttattcttct cgcgtggaat gcccacgtag gccaacacat tttgcggcac
     2641 gaaaacaaaa gccagtccga acatttgcaa cgtgcgcatc aaaaacgccg cgcggaaatc
     2701 catcgtaagg ctgaataagg tggacatgta atacattgcc gcggaaatgg aaacgaatcc
     2761 gaaagccatc aacttgcgcg gatcgatgcg cgaaatcatg gtccccacaa aaggcaacac
     2821 catgatcata ataaacccgc cgcccgccag cgcttcgccc gccaccagcg cagggtaacc
     2881 cagctgcacc tgcaagaatt gcggaatcat gaacgtcgtg ccatacagca cgatgcccac
     2941 cgtaaaactg aaaaacatgg ccgtggcgaa atttcgtcgc ttaaacagac gaatatccac
     3001 gaccggattc gggtggtacc actcccaaac gatccagacc agaaacgcat agccagcggt
     3061 caccacgagc gatgtaatcc agcgcgagga aaaccagtcc gactcttgcc ccttgtccag
     3121 cgcgatctgc aaactggcaa tggccaacgt aatcagtccg aggcccacgt aatcggcgcg
     3181 gattcctctt ctcctctctt gctcgcgttt caagaacggc gggtcttcca ctagtcgatt
     3241 ggtaaggaac agggagagca agccgatggg gacgttgatg tagaaaatcc agcgccaatt
     3301 gtagttatca gtgatatacc cgccgagcgt cggcccaatg gcgggtgcca acactacggc
     3361 catggcatag acggcaaagg ccatgccacg tttggcagga gaaaacgtat cggcaaggat
     3421 ggcctgctcg ctcggctgca atccgccgcc gccaacgccc tgcaagattc gaaaaaagat
     3481 cagcacgccg aggcttggcg caagtccgca aagaaaactg ctcatcgtaa acagcgcgac
     3541 gcaggacatg taaaagcgtt tgcgtccaat gatcgtcgaa agccatgcgc tgatcggcaa
     3601 gataatggcg ttcgaaacga ggtagctggt gaggacccag gcggactctt cctggcttgc
     3661 cgaaaggctt ccggcaatat gcggcaagga cacgttggcg atggaggtat cgagcacctc
     3721 catgaacgtg gccagcgtaa ccgtcatggc gatcagccaa gggttatatt ttggcttcca
     3781 gggagcttcg aatggttccg tcacgttctt ctttctgtaa cgcagaattg cgtagcacag
     3841 acttcagtct gtggggggtt acgttcgaaa gttctatcga cgcaaattta gccaagatca
     3901 aatggaatgc cgcagctgaa gtctgagcta cttcagccac accttcggga caactgacat
     3961 tcccggacgc aaacgatcga ggccttgctg gccttctttg aatcgaattc gaaccggcaa
     4021 tcgctggacg actttcacgt agtttcccgt ggcattttcc ggaggaagca aactggtaat
     4081 cgaaccggtc gatccaggca tggcttcgac cgttccgtcg aaatcctgat cgaaggcgtc
     4141 cacggaaatc ttgacggctt ggccgggatg catttcccgg agctggcttt ccttgaagtt
     4201 ggcggtcacc caaagattgc cgagatcgga gacagtgacg agccgctcgc ctttgctgac
     4261 gtgttcgccc acttccgcag tccgcttgct gacaactccc gagacgggcg cgacgatctt
     4321 gcaatagctc aaatcaagtt gcgcgcgatc cagctgcgcc ttcatcgcct caacgttggc
     4381 gtgtttcgat tgcaaagtcg cgcgggaaat ggcgacctga ttgggcgcgt ttacagcagc
     4441 tgaagccatc aggctgcgcg cctgctccag ttgcgagcgg cgttgctcga ccactttttg
     4501 agccgccagg gccgaggcgc gcgcggaatc caccgaagcg gcaagcgcgt tggcatttgc
     4561 cactacctgg tcgtaatcgg aacgggaaac ttcattcttg tccacgagcg ctttgtaacg
     4621 cgcgacatcg gcttgcgcct ttccgttatt cgcttcggat tcgagtaaac gggattgcgc
     4681 cgccaattgt tcgccttcgg ctgctgcggc ggcggcttcg gcggtggcta cttccgactt
     4741 gctggtggaa acgctcgtct gcgaagtgct ctgcgtaatt ggaagattcg gattttccgc
     4801 gcgctcgtcg gcctcagcct tcataacctg tgcctgcgct tgctcgaccg cgacttcgta
     4861 atcgcgcggg tcgagttcga caagcacatc tccggctttt accgactgat tttcttccac
     4921 atgaactgct ttcacgacgc catcaatccg cgccgtaacg ccgttcagat gtccgtccac
     4981 ctgcgcgtca tccgtggttt cataagtgcg ggcatacagc cagaacccta gcgcggccac
     5041 aagaattacc gcgcctatga tggccgcgaa taaaaatgtc ttgcgccgcc cctccacact
     5101 gcttccggtg tccacaggct cgacagtacg ggcttccctg gtttctggta attggtcaac
     5161 ggccatggtt attggctcct caaaagatca cgcataattt tttccgcctg acctgtggcg
     5221 cgggccacgc ttgccttggc caggttatgg gcattcagat cctcgatgta gtctgactcc
     5281 gcgctggcaa ctacttcctg ggcctgagtg acttcgacgg tgtccgcaac tccggaagca
     5341 aaacggtccg tcgattgcgc gagttcatcg cgcgccagtt cacggcgact ttgcgccacg
     5401 cgaacctgct cggaggcggc gttcaggtcc agatacgcat tcctgacatc ggcttccacg
     5461 cgggaacgaa cgctctcgta ctcggcgcgc cgctgcgtca gatttgcatc cgcttccaca
     5521 atgtcgccgt gcgtatgccc gcctgcccat atcggaaaca taagctgggc gcttacgtca
     5581 aaagtgccat gcgaatttgc aggatttatt ccgatcacgc cgtaatccgc cgtgactccc
     5641 agcgtgggat agtgctcggc tacagcagcc ttgcgcgcaa attcagcgga gtgcagctgc
     5701 gcttccgcag ctttcaggtc agagcgattt tgcgcggcca gtgtgagtgc ttgctcgact
     5761 gtcaaattat ccagcggggc gtagggcaat ttatccgcaa gcgaatacac ctgcgcgggt
     5821 gacatcccaa tcaatcgcgc cagtccaatt ttctgcttgg cgtaatttgc ttgttccgag
     5881 gtcaagcgct gctgctgcgt ctgcaattcg acattgcttc tggtcatgtc gatttttgcg
     5941 ctgagcccgg cattaaagcg atccgacgcc tgttgaaaga ttgcctgcgc gctatccact
     6001 tgggctttca cggactccac acgggccgat tcagccagca cctgcacgta cgcctgcgtc
     6061 accacaaaga ccacgatttc gcgcgaatca gccatggaga gttccgcggc gcgctgcgct
     6121 tcacgggcag actggtaggt gcggatcgat tggacgtcgg cgatgcgctg agtcagataa
     6181 ccgcgcaaat cgaaaaaatt gaacggcccg acgatattcg gaaaattaag tcccaagctg
     6241 ggaggaaatt tgaatttaaa cccgtaagaa gccagatcga tttgctggac attctcgcga
     6301 acgccgccgg agaagtccgg aaacagcgcg ctgcgcacga caattttttc tccggttgcg
     6361 cgctcggaag cctgtgccgc ggtaatgccc cccaaattat aacgcaggcc cattttcagg
     6421 gcctggtcga gagacagcga aactggctga tccgagggcg gacccgcgct cgcgctgccc
     6481 tggtaaggac cggtttgcgc caccgcgcag gttgcagcgg cgaatacgcc catcagcaga
     6541 aggaacagat agaaccggaa attccgcatg tattgcgctc tattcttcgg gacaagccgc
     6601 acgtcgcgct cgcaattcag aatccagagg ggaggcgcgg ccggctatcc gattatgcaa
     6661 ttaaagcttg cgtcgacggg aacgcccaat taagatacat acacgtatgt atcatgtcaa
     6721 ggaagcgatt tattgagatg aagggtattt ccatgagcgc acgcggcaaa agaggccgga
     6781 cacgtcccac cgaggacgcc cggactcgca tgtgccagga agagcgcacg cgagcgaccc
     6841 gccggaaact cctcgatgcc gccaagcgca tcttcgcaca ggatggcttt gaagccgcgc
     6901 gcctggaaga tatcgccgcc cgcgccggat acacgcgagg cgcgttctac gccaatttcg
     6961 agagcaagga agacattttc tttgcgctgt ttgaggagtg ggtacgcgaa agaattgagt
     7021 ccgtcaccac tgctctgcgg tgccatcccg atcccgcgga aaagcttgcc gcgctgcgaa
     7081 ctcactatgc gaaactcgcc acggaccgcc gcctggttct gatttccatg gaattcaaat
     7141 tatttgcgct gcgccacccg gaagcgcatg cccggctgag aagcaggcac cgccggattc
     7201 gcgcgacgtt tggcgaactg ttttctgaat tgatgcgcga gctcgatacg accatttcca
     7261 tcgcctatcc ggcatcatcg gcttgcctcg gtgccgtggc gcaaggtctg ctgctggaac
     7321 atttaatcga tccgaagacg ctatcggatg gtgatgttcg ccatgcgctg gggcagtttt
     7381 tcgattccat atttcaggct gttccggcat agcacaggca agcatgaccc aggcaattgg
     7441 gtggaaggaa aatgtaacgg gattttgccc cgtcgcatca ataaatatgg aatctgccaa
     7501 caatgggaac atgagaagat ggcaaacgcg cctcataaat gtcccggcct gcccggcggg
     7561 gaaagaacag cgcagccgca aataaacttc tgaatcgccg gcaacggtca gatttcaagt
     7621 gcgcaaaatc gtttagagat cgatttattg gcgcggtaac caacgattga tgtatctgtg
     7681 tcggaaaagc tcttcgcggc ccaggtaggg agctagaaaa ccagaatgtg gattgttcga
     7741 ttagccctca gaaggccata taccttcgtg gtgatgtcct tgctgattct gattctcggg
     7801 acgctgtctg tgtttacgac gcccgtcgat atttttccaa atatcgatat cccgatcatt
     7861 gccgttgtgt attcgtacgg cgggctttcg gccgaagacg tctctcagcg cctcgtcgtc
     7921 aactttgagc gtggcctcac cgcgacggtc aatgacgtcg agcatatcga atcgcaatcc
     7981 ttaaatggac tcggcgtcat caagattttt ttctatccca atgtacgcgt cgatatggcc
     8041 atggcgcagg ttacgtccaa cgcccaatcc gccctgcggg gagatcctcc tggaacgact
     8101 ccccctttcc tcctggcgta caacgcttcc tcggttccca ttcttcaact cgcaatttcc
     8161 ggaaaaggtc tttccgagca gcagttgaat gatatggggt cgaattttct gcgcacgcaa
     8221 ttggctaccg tacaaggcgc cgtttttccg aacccgtatg gaggcaagca gcgcgaaatc
     8281 atggtggacc tgaatattcc agcgcttcag gccaaagggt tgtcgccgga cgacgtagtc
     8341 accgcgattg ggaatcagaa tctgattctg ccagctggaa ccgtaaaaat cggaaccaag
     8401 gaagacgatg tggacttgaa tggaagtccg caaaccgtaa atgaaatgaa tgatctgccc
     8461 atcaagacct cgggcagcaa tacgatttac gttcgggacg tggcccatgt ccgcgatggt
     8521 aaccctccgc aaaccaatat ggtgcttgtg gatgggcaaa aagccgcgct catgactatc
     8581 ctgaagcttg gcagcacttc tacactggat atcattaaac aaatcaagga acgcctgccc
     8641 gtggtgctcg ccggcctgcc gccggagttc aaagcgcgtc cgattgccga tcagtccctt
     8701 tttgttcgag cttcgatcaa tggcgtggtc cgggaggcgg tgatcgcggc gtgcctcacc
     8761 ggcctgatga ttttgatttt cctcggcagc tggcgaagca cattggtcat cgcggtttcc
     8821 attcctctgt cgattttgac ttcgattatc gtattgggcg caatcgggca gacgatcaac
     8881 atcatgaccc ttgggggctt ggcgctggcg gtgggcattc tggtcgatga cgcaacagtg
     8941 gaaatcgaaa acatcaaccg aaacctcgcc gaaggaaaag aaacgattca agcaattctg
     9001 gacggcgcac agcaaatagc cgtgccggcc ttcgtttcca cactttccat ctgcatcgtg
     9061 ttcgtgccca tgtttttcct gacgggagtg gcgcgctatt tattcgtgcc cctggctgag
     9121 gccgtggttt ttgcgatgct ggcctcgtac ctgctttccc gcacgctcgt gccgactatg
     9181 gcgcgttacc ttttgaaagc gcatgcggaa caatcggcgg aagcgtcgct tgccagcaag
     9241 aatcccctgg ttcacctgca gggacttttt gaattgcaat ttgaaaaatt gcggaattct
     9301 taccgggaac ttttgagatc ctgcctggcg cttcgagttg cgttcccatt gatcttcctg
     9361 gccatttgcg ttggatcttt tgttctatac ccgtggctgg gccaggattt cttccccagc
     9421 gtcgatgccg gccaaatcaa attgcatctt cgcgcgccca ccgccacgcg aattgaagac
     9481 accgcggaac tctgcagtga agtggaacgg gcgatccacg agcaaattcc ggcgaatgaa
     9541 atcgaaagca ttatcgacaa tattggcctg ccatacagcg catacaatta cgtgtacact
     9601 acttccgcac cgatcggccc ggaagacgcg gacatcatga tttcgctgaa cgagaaccat
     9661 caccccaccg aacaatacat tcatgatttg cgccagaaac ttaataaatc ttttccaggc
     9721 gtgacgttct acttcccgcc tgcggatatc gtcagccaga ttctaaattt tggcctaccg
     9781 gccccgattg acattcaaat catggggcgc gatgtggagg cgaaccacga gttcgccaac
     9841 aatctgctga agcaattgaa tacggtcaca ggaattgccg acctgcggat tcagcaacct
     9901 ttcaatcagc ccaagctgca catcaatgtg gaccggacta aggcgctgca ggccggattt
     9961 actcaacgcg atgtggccgg aaatttgctc gtttccctga gcggaagctt ccagacttcg
    10021 ccgtccttct ggcttgatcc taaaaccggc gtcagctata tcgtggccac ggaagcgccg
    10081 cagtatcgga ttcagagtct tcaggacctt gagaacattc ctgtaacagg tggcggaggc
    10141 ggaggaggaa caggccgccc gccgtggcaa cagcttgcca atctcggaac cgttacgcgc
    10201 ggtgaaggaa tggctaccgt gtctcactat gacgcgcaga cgatgatcga tattttcggc
    10261 gcaacgcagg gtcgcgatct tggcggagtg gccaaggaca tcggtaagat tgttgatgcc
    10321 aatcgctcca aattgccgcg cggctcgcaa atgtttgtcc gagggcagat cgatacgatg
    10381 cgcagttcgt tcactggcct tatcggcggc cttgggtttg ccattctgct catttacctt
    10441 ttgattgtcg tcaacttcca atcctggctg gatccattca ttattatttc cgcactgcct
    10501 gcggctttag cgggaattgt ctggatgctg tttattacgc acacgacgat cagcgtccct
    10561 gcgttaaccg ggtccatcat gtgcatgggt gtggcgacgg caaacagcat tctggttgtc
    10621 agcttttcca aggaacgcgt gcaagagggc cttactccgc agcaggctgc ggaagaggcc
    10681 gggttcactc gctttcgtcc cgtgatcatg acggccatgg caatgattat cggtatgctg
    10741 ccgatggctg tgggtctcgg tgaaggcggc gagcaaaatg cgccgctcgg acgcgcggta
    10801 attggcggtc tgatttttgc gacggttgca acgctcttgt ttgtgccgtc ggtatacagc
    10861 ctgattcacg ggttccgcag agagccaggc caatccgctg aaatcgaaat tcctggcgag
    10921 taacgatggt taatttttcg gaggatcaga tgggggttgt ttccaaacag agggaacagg
    10981 cgcaatcgcc ggcgggtgcg cgggcgaata tctgtgccgt atcttctcga ggcagcgcaa
    11041 tgacgcgtat cgtaacgctt ttgctgattg ttattgttgt cgccattatt gtgctttggg
    11101 ggatttccag ccgccgcgcc gcgaatgctc agctgtccca ggaa
//
LOCUS       DASZMB010000237        12742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4368248, whole genome shotgun sequence.
ACCESSION   DASZMB010000237 DASZMB010000000
VERSION     DASZMB010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 12742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12742
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4368248"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VG272_11185"
     CDS             <1..320
                     /locus_tag="VG272_11185"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HWF14291.1"
                     /translation="ALKPLANVSAWLSHAEPAREALVTPETDLADPEFLLKLTERNVV
                     EQLKNLHTHPSVAGRLERGDLKLHGWVYHIGKGTVTTYSKRKEKFVRIRTVKPRKEAR
                     ARR"
     gene            545..1618
                     /locus_tag="VG272_11190"
     CDS             545..1618
                     /locus_tag="VG272_11190"
                     /inference="COORDINATES: protein motif:HMM:NF014110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14292.1"
                     /translation="MNRIVAVVIVYLAAALPCIAQTASPERMGQTWTDSRSGLTSQNV
                     RQLAVLGPHGQTVFAVGLYGGLFKSADYGEHWNPVSGLPPGKIWAVTADATGSRMIFI
                     MADAGIYQSRDAGTVWSRVPLDDDSTVSSLPALDSSLPTVIRADFRDRILYSLDGGKT
                     WSLFKARLPISDPFPSIWIHPHNSQFLFASGLIGGLYSSRDGGQTWNQISEAPRSLNY
                     AFFLDVNQPETMYMLTHEGSFPSHTYKSTDNGFHWEPMIVDVPLDASPEFLAMIPSNP
                     TVLFMSIADESSCCPRPTTLFRSVDMGSHWSRADSGLPKGTGVGPIASAPDDPSVLYL
                     GVGGAGVFKSTDGGEHWRPTVAR"
     gene            1835..3274
                     /gene="proS"
                     /locus_tag="VG272_11195"
     CDS             1835..3274
                     /gene="proS"
                     /locus_tag="VG272_11195"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HWF14293.1"
                     /translation="MSDQKLPSRSEDFSEWYNQLVLRAELADYAPVRGCMIVRPYGWS
                     LWENITAQLDRRFKATGHVNAAFPLLIPQSFISKEQSHVAGFSPELAVVTHGGGEKLE
                     EPLVIRPTSETIIGWAYSKWIQSYRDLPVLINQWNSVVRWELRTKLFLRTLEFFWQEG
                     HTAHATYDEAEAETRQMLDIYTDFAVNEAAIPVIPGRKSQSERFAGADATYSIEAMMG
                     DGKALQSGTSHNLGQNFAKAFEIKYLDQGGTLQHCWTTSWGLSTRFVGAIIMVHGDDQ
                     GLILPPRLAPHQVVIVPIFRTDEEKALVFEAAKKIREQLVKADIRVKLDEREGQSPGF
                     KFNDWEMRGVPLRMEVGPKDVAKGTVVLARRDKPGKEGKTFVSQEGIAQSVADLLVTI
                     QKALFDRAFAFRNANTFEPKDLTEFGEAVERGFARSRWCGDAKCEEQIKDTTKATLRC
                     IPLEQPGGSAGCIFCGKPSAEVAIFGKAY"
     gene            3518..3886
                     /locus_tag="VG272_11200"
     CDS             3518..3886
                     /locus_tag="VG272_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HWF14294.1"
                     /translation="MKRNSEKGAGHMKAIIWTMILVAFIYVMVMTIPVLINEYQFQDS
                     LDEIARFSSVGHKTSAEIQKAVLEEAQKEDLPIQAEDIQVAGAGGNVHINVSYSVTVD
                     LKVYQLTLNFHPAASNSAIL"
     gene            4136..6679
                     /locus_tag="VG272_11205"
     CDS             4136..6679
                     /locus_tag="VG272_11205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HWF14295.1"
                     /translation="MRIRIGRGKWVPRLSLFIFGAAVLFLLVGIGIGTYYWVSFGRMI
                     DQRLSGHIQQTTARIYAAPMRIYTGEALTVADMANHLERAGYSELDVSGTPGRYVLHG
                     NEIEIRPSADSYFGATNRLVVDFSKAGIEKIRSMDTGGALDSAEVEPELLTSLFDSSR
                     EKRRAISYNDIPRVLRDAVLSVEDRRFFEHPGFDPVRILGAAWADLRHGARVQGGSTI
                     SMQVARSFFFNTDRTIRRKIAETVVALELEHRFTKQQIFELYANEIYLGNRGSFAIHG
                     FGEASLAYFNKDLREVNLQEAAFLAGIIHAPNRYSGSERRPERAIEARDRALLAMTEN
                     KAITAEQAQTAKKIPLQIVGSGLEGSTAPYFVDMVKDHLLDRFSEADLLSQSFRVYTT
                     LDPELQRAASEAMDIGAKNIDAQLARRYAKWRKAGQPAPQAQIAMVVLDPHTGEIKAL
                     IGGRDYGESQLNHILSRRQPGSAFKPFVYAAAFQGGVDGVQPIITPATTVMDEPTTFD
                     FDGKEYTPNNYGEKFHGQVTVREALTYSLNVATVKVAELVGYTRVTDMAHEFGLDPSI
                     QPTPSVALGAYEMTPLEVATGYTILANDGVRTEPMFIRNVVNAKGESLEENAIRSRRA
                     LDPRVAYLVTSMMEDVINHGTGATVRARGFNAPAAGKTGTSRDGWFAGYTSNLLAIVW
                     VGFDDNRDLGLSGANAPAPVWAEFMKRAVALPAYRDVKPFDMPDGVTKVTIDPETLAL
                     ATPECPTSREEVYIHGTEPTEFCPLHGGHMANDTAPGSWLSHVFGGDKNKNGEAAPPA
                     PNSHPSSTAPAAAENQQPEEENKKGVLRRIFGIFGGKKDADKSQSGPQD"
     gene            6714..7556
                     /locus_tag="VG272_11210"
     CDS             6714..7556
                     /locus_tag="VG272_11210"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF14296.1"
                     /translation="MKMGSPSPRISVGLLFFVLSTGLGAHPSVKNQAPQSSQSQSNSG
                     TTPTLSPKEMQVERAKVQMAEKRFEAAIQTYQDLLKADPKNASYMNMIGIAYLDLGRN
                     DFAKKYFLRASKADKKNSNAVNNLGMVYYHQKDFRRAIREYQRAITIDPGQAGPHANL
                     GFAYYNSNKFPEAAIEFQKALEIEPSIFERNDKVGSSVQDRSVSNHGLFFFTMARVYA
                     QKKDAEHCAEYLRKSLDEGYKDVGKAATDPAFKDVVDDPAIQAVLVLIAKPDSKAAPA
                     QPGA"
     gene            7795..9021
                     /locus_tag="VG272_11215"
     CDS             7795..9021
                     /locus_tag="VG272_11215"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF14297.1"
                     /translation="MANLDWLGEGLSELTAERFEDRGVNVLSREDRLATLERMGLPDS
                     ARFSHATIVKIATEADADAVIFGRFQYDGQKATLEARVLRISPPSLSPAFTETGSMED
                     LLRAHARLTWKVLCAVNKKNCPLQGASQDESSFSEPPPSLRPEALENFIRGVTGSQDE
                     ERLRLLREAARLEPNWDRPAFELGKIYFKRHDCDSALVWYSRVPPNRPDGPEASFATG
                     VCHLARNDATRAEATFSGLLERTRRTGEKDWLPELPEMRNNLGVSRLQIGKWNEAETE
                     FERASALDPEEADYWINIAIAKLIGKQAPAAVTALDHARKIAPDDKEARVLSIATLDS
                     LGRSTEASAMRAEVTEKGDKQVMPNLQDGVALARMARVSRSFDRTLLRPGGEAPEGQS
                     PAGKGPRTSNDSGVRP"
     gene            9216..10433
                     /locus_tag="VG272_11220"
     CDS             9216..10433
                     /locus_tag="VG272_11220"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HWF14298.1"
                     /translation="MAEYVDSGIEEAARRHASLVLITMDTPGGLSTSMEDIIHNILVS
                     PVPVAIYISPAGSRGASAGFFILLSADIAAMAPGTHTGAASPLLAIGGFPVQVDETLK
                     KKILNDATAFLRSYSAKRGRNVELAESAVVDGKAWTETEALDGHLIDLVANSQDDLLA
                     KLDGRTIKRFDGSTVKLELHNPVYVPFTMSFRQQFLSRIVQPDAFFILLIVGVLGLYV
                     EFTHPGMVAPGVVGGIALVLALYAMHILPVAPAGIVLIILALGLFIVEAKYTSHGVLA
                     IGGIVSMLLGALMLVRSPLTHAGVSLGVALGVTLPFAIIVIFLMRLVLKSRSWKPSMG
                     REQFVGNVAEVTSSIVKTAEEGMFEGMVRLNGALWRAVAREAIPGGSHVRVVSFEGLT
                     LHVVPVEHSALAK"
     gene            10496..11275
                     /locus_tag="VG272_11225"
     CDS             10496..11275
                     /locus_tag="VG272_11225"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HWF14299.1"
                     /translation="MDLSSFSLYGVMIALFIGFIWFWNSVNVLKEWERGVVLRLGRMM
                     PNAKGPGLCLVFYPIETLTRVSLRIETLDVPSQDIITRDNVSVKVNAVAYFAVVDANL
                     AVSRVQNYMYATSQKAQTTLRSIVGQFELDEILAEREKVNSKLQVILDQDTEPWGIKV
                     TKVEVKQVDIPEQMQRAIGRQAEAERERRAKVIHAEGEAQASAKLAEAAKVLETQPAA
                     IQLRYLQTLTEIGVEKNTTIVFPLPIDIMNEWVKAIGSRKQ"
     gene            11276..11929
                     /locus_tag="VG272_11230"
     CDS             11276..11929
                     /locus_tag="VG272_11230"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HWF14300.1"
                     /translation="MASNTKKSGSDFVLESRHLVGLFLLLVVIFGVVFTLGYLMGRSQ
                     GDSRSRTVIAAPAEPSTSAAVPPSSKSKAKPAPPPDDTQTQKTNSDWDFYHSADPQAN
                     SDRLQPPSKAAAPPAAMSKPAPSGKAAKQGKAPPGLGGPLIPKGTIMLQVAAVLHQDD
                     ALALAQALQKKKFPAIVIPPNADKYYRVQVGPYADNQAAANARTDLEANGFKSITKR"
     gene            12045..>12742
                     /locus_tag="VG272_11235"
     CDS             12045..>12742
                     /locus_tag="VG272_11235"
                     /inference="COORDINATES: protein motif:HMM:NF039484.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14301.1"
                     /translation="MNSSRTVRLLLALVSGVALALAYPLFNLPILGWIAPAVLIIAVI
                     GERPRFAIVLGFVQGAVYFGLSLPWFYNVMRQYGPLPVAQAAGVFLLVVLVLSIFHAV
                     FAAAIAWLGKASSKRACLAAPFLWVALEFGTMYMPAIGFPWNLLGYVAAGNLAFVQIT
                     AITGIFGLSFLVAAYSSLLAWAVLQFSRYKKTEWKLLGGATATLIVIALAGPQFVPKA
                     SADHVAHLVQTNFPV"
BASE COUNT         2889 a         3308 c         3644 g         2901 t
ORIGIN      
        1 aagccctgaa gccgctggcg aatgtgagcg cgtggttgag tcacgccgaa cctgcgcgcg
       61 aagcgctggt cacgccggag acagatttag cggacccgga atttctgctt aagttgacgg
      121 aacgcaacgt ggttgagcag ctaaaaaatt tgcacacgca tccgtcggtt gcgggacgcc
      181 tggaacgcgg cgatttaaag ctgcatggct gggtttacca cattggaaag ggaaccgtga
      241 ccacttacag caagcggaaa gagaaattcg ttcgtatacg tacggtcaaa ccacgcaaag
      301 aggcaagagc acggaggtaa actgttagag gaaagaatat taagaaatgg ctcgtgagtc
      361 ctcccactaa gatgaagaca cgaaatttgg gaactgagaa attcccagtt gatgagaatc
      421 ccgtttcaca ggagttacga tgagggggcc ataattttgg aaccatgtcg cgccaggcag
      481 attgacagcg actagacgcc acgcgatgct ggaacttaaa gggaagggct actgagtcat
      541 tgccatgaat cgcatcgtcg ccgttgtgat cgtttacttg gccgccgcgc tgccctgcat
      601 cgcacaaacc gcaagcccgg agcggatggg acagacgtgg accgattcaa ggtcgggcct
      661 cacatcacaa aatgtgcgtc agttagccgt tcttggtccg cacggccaga cggtattcgc
      721 agtcggcctt tatggcgggc ttttcaagag tgccgactat ggcgagcatt ggaatccggt
      781 cagcgggcta ccgcctggaa agatctgggc cgttactgct gatgcgacag gtagccgcat
      841 gatctttatc atggccgatg ctggaatcta ccagagccgc gatgcgggga ccgtgtggtc
      901 tcgagtcccc cttgatgacg actcgaccgt gagttcgttg ccagcgttgg attcgagtct
      961 gcctaccgtc attcgtgcag actttcgcga cagaatactc tacagcctgg atggcggaaa
     1021 aacgtggtct ctcttcaagg cgcgcctccc gatttcggac cctttccctt ctatatggat
     1081 tcacccgcac aattctcaat tcctttttgc gtccggcttg atcggagggt tgtatagcag
     1141 ccgtgatggg ggccaaacgt ggaaccagat ttcagaagct ccaagatcct tgaattacgc
     1201 atttttcttg gatgtcaacc agcccgaaac gatgtacatg ctaactcatg aaggcagttt
     1261 tccaagccac acttataaat caaccgacaa tggcttccat tgggaaccga tgatagtgga
     1321 tgtcccactt gatgcatccc ctgaattcct cgcgatgatt ccgtcgaacc ccacggttct
     1381 cttcatgagc atcgcggatg agagcagttg ctgtccgagg cctaccaccc tattcaggag
     1441 cgtcgacatg ggttctcatt ggagccgagc ggactcagga ctgccaaaag gcaccggtgt
     1501 cgggcccatc gcctctgcac cagacgatcc ctccgtcctg tatttgggag tcggaggggc
     1561 gggagtcttc aagagtaccg acggcggaga acactggcgt cccaccgttg cgcgataatt
     1621 tcgaagagtg acgccctatc gtgccaagtc atgataagtc ggcttctcgg aaactggctt
     1681 gccacttatc ccggagttgg ttgacacctc tggcaccctc ggctacgctt ctttttcttg
     1741 actcatgcca aattgggcaa ctgtgtcgcg ctgttcttaa tggatgtggt agttgaaatc
     1801 aaagacgaat gcaatttcct aaggataggt gcacatgtcg gatcagaagc ttccttcgcg
     1861 ctctgaagat ttttcggaat ggtacaacca gcttgtgttg cgcgcggagc ttgcggatta
     1921 cgcgccggtg cgcggatgca tgattgtgcg gccttatgga tggtcgctgt gggaaaacat
     1981 tacagcgcaa ctcgatcgcc gcttcaaggc gacggggcac gtgaacgccg catttccgct
     2041 gctgatcccg cagagtttca tcagcaagga acaatctcac gtggcggggt tttcacccga
     2101 gttagccgtg gtcacgcatg gcggcggaga aaaactggaa gagccgctgg tgattcggcc
     2161 gacgtcggaa acgattatcg gctgggcgta ctcgaaatgg attcagtcgt atcgcgatct
     2221 tcctgtgctg atcaatcaat ggaatagcgt ggtgcgttgg gaactgcgca caaaattatt
     2281 tttgcgcacg ctggaatttt tctggcaaga aggacacaca gcgcacgcga cctatgatga
     2341 ggctgaagcc gaaacgcgac agatgctcga catttatacg gatttcgcgg tgaatgaagc
     2401 ggcgattccg gtgattcccg gcagaaaatc acagtccgaa cgcttcgccg gcgcggacgc
     2461 cacttattcg atcgaagcca tgatgggcga cggcaaggcg ttgcaatccg gcacatcgca
     2521 taatttgggc cagaatttcg cgaaggcatt tgagattaag tatctggacc agggcggcac
     2581 gcttcagcat tgctggacta cttcgtgggg actttccacg cgctttgttg gcgccatcat
     2641 catggtgcat ggcgatgatc agggcctgat tttgccaccg aggcttgcgc cgcatcaggt
     2701 ggtgatcgtg ccgatcttca ggacggacga agaaaaggcg ctggtgtttg aggccgcaaa
     2761 gaaaatccgc gagcagctgg tgaaggcgga tattcgcgtg aaattggacg agcgcgaggg
     2821 acaaagcccc ggcttcaagt tcaacgattg ggaaatgcgc ggagttccgc tacggatgga
     2881 agttggcccg aaggacgtgg ccaaaggcac ggtggtgctt gcacgccgag acaagcccgg
     2941 caaggagggc aagacattcg tatcgcaaga gggcattgcg caatccgttg cggacctgct
     3001 cgtcacaatt cagaaagcac tttttgaccg cgcgttcgcc ttccgcaacg cgaatacgtt
     3061 cgagccgaag gatctgacgg aattcggcga ggctgtcgaa cgcggatttg cacgctcacg
     3121 ctggtgcggg gatgctaaat gcgaagagca aattaaggac acaacaaaag ccacactacg
     3181 ttgcattccc ttggaacaac ccggtggatc ggccggatgc atcttttgcg gcaagccatc
     3241 agcggaagtt gccatcttcg ggaaggcgta ttaagggcga agacttgcca agcctttccc
     3301 acttctatat ttcctcgtaa gtcacatgta tgcattggtt tgtggaaagt tactgccccg
     3361 aaaccgctgt ctctctctct ctcgacactg ctgctgaccg atgctagcat tttaatctat
     3421 gaaacgattg ttaccgccat gtgtgcctga aacacccatg ttgtgcctag atggcgcgaa
     3481 aaagaaattg cgcgcgcttt ccgtggaatc tcccgttatg aaacgcaata gtgaaaaggg
     3541 cgcggggcac atgaaggcca tcatctggac catgatttta gtggcattca tttacgtcat
     3601 ggtgatgacc attccagtcc tgatcaatga gtaccagttt caggatagtc ttgatgaaat
     3661 tgcgcggttt tccagcgtgg gccataagac gagtgccgaa atccagaaag ctgttctaga
     3721 agaagcgcag aaagaggatt tgccgattca agccgaggac atccaggtgg cgggcgccgg
     3781 tgggaatgtt cacatcaatg tcagctactc cgtgacagtc gaccttaagg tctaccagtt
     3841 gacgctgaat ttccatccgg ccgcaagcaa ttccgcgatt ctttagcgct ttcgcgcgag
     3901 tgataatcga gtgatcagaa tgaggcaatc ggttgcgcag attcccagcc tcgagccgag
     3961 caattgcctt ttatcggcga ctgtacctcc gagaaaacga gtcgcattcg taattgatcg
     4021 ccgcgacggg taaaatagtt ccggttcagc cggtaagcgc atctaacact tggtagacaa
     4081 aaaggtcatt cagaggggca tcgggcgttt tttcgcggcg gaacggagaa ccatcttgcg
     4141 tattcgaatt ggccggggta agtgggtccc gcggcttagt ttgttcatat ttggagctgc
     4201 agttcttttc cttctcgtag gcattggaat tgggacgtat tactgggttt ctttcgggcg
     4261 catgattgac cagcgccttt ccggccacat tcagcaaacc accgccagga tttatgccgc
     4321 tcccatgcgc atctatacgg gggaggcgct tactgttgcc gacatggcga accatctaga
     4381 acgcgccgga tatagcgaac tcgatgtttc gggaactccg gggcgctacg tcctgcacgg
     4441 aaatgaaatc gaaattcggc cctctgctga ttcctatttt ggagcaacga accggctggt
     4501 ggtcgatttt agcaaggctg gaatagagaa gattcgctcg atggacaccg gtggcgccct
     4561 cgattccgcg gaagtagagc cggaacttct gaccagtctt ttcgacagtt cccgagagaa
     4621 gcgtcgagcc atcagctaca acgacatccc aagagttttg cgcgacgcgg tcctatcggt
     4681 ggaggaccgc cgcttctttg aacatcccgg atttgaccct gtccgcattc tgggcgcagc
     4741 gtgggcggat ttgcggcacg gtgcgcgcgt gcagggtggt agcacgatct cgatgcaggt
     4801 ggcgcgcagc ttcttcttta acaccgatcg aaccatacgc cgtaaaatag cggaaaccgt
     4861 ggtggcgctc gaactcgagc accgcttcac gaagcagcaa atattcgagc tgtacgccaa
     4921 tgaaatttat ctgggcaacc gcggcagctt cgcaattcat ggattcggcg aagcgtcact
     4981 tgcttatttc aataaagatt tgcgcgaagt aaatctgcag gaagccgcgt ttcttgccgg
     5041 gattattcat gcgccaaatc gctattccgg ctccgaacgg cgaccagagc gtgcgataga
     5101 ggcgcgcgat cgtgcgctcc tggcgatgac ggaaaacaaa gcgatcactg cggagcaggc
     5161 tcaaaccgcg aaaaaaattc cgctgcaaat tgtgggcagc gggttggaag gcagcacggc
     5221 cccttatttt gtggacatgg tgaaggacca cttactggac cgcttctctg aagccgatct
     5281 tctctcccaa agtttccgcg tctacaccac gctcgatcct gagttgcagc gcgccgcaag
     5341 tgaggcgatg gatatcggcg ccaagaatat tgatgcgcag cttgctcgac gctatgcgaa
     5401 gtggcgcaaa gcaggccagc ccgctcccca ggcgcagatc gcgatggtcg ttctggatcc
     5461 tcacaccggc gaaatcaagg ctctgatcgg cgggcgcgat tatggcgaaa gccagctcaa
     5521 tcacattctt tcgcggcggc agcctggctc cgcgttcaag ccattcgtgt atgcggcggc
     5581 tttccagggg ggagtggacg gtgtgcagcc catcataact cccgcgacga cggtgatgga
     5641 cgagccgacg acgtttgatt ttgacggcaa ggaatacacg cccaacaatt acggcgaaaa
     5701 attccacgga caggtgaccg tccgcgaagc gctaacttat tcgttgaacg ttgcaactgt
     5761 gaaagtggcg gagcttgtgg gctatacgcg cgttacggac atggcgcacg agtttgggct
     5821 cgatccatcc attcagccga ctccctctgt ggcgctcggc gcatatgaaa tgacgccgct
     5881 tgaagttgcc accggttaca caattctggc gaatgacggc gtgcgcacgg aacccatgtt
     5941 cattcgcaat gtggtgaacg cgaagggcga atcgctggaa gaaaacgcaa ttcgatcccg
     6001 gcgggcgctc gacccgcgtg ttgcttacct tgtgaccagc atgatggaag acgtgatcaa
     6061 tcacggcacg ggagcgacgg tccgcgcgcg cggattcaac gcacctgccg cgggcaaaac
     6121 cggaacttcg cgtgacggat ggtttgcagg ttacacatct aatttgctgg ccatcgtgtg
     6181 ggtcggattc gatgacaacc gagacctcgg cctttcgggc gcaaatgctc ccgcgcccgt
     6241 gtgggcggaa ttcatgaagc gcgcagtcgc tctgcccgca tatcgcgacg tcaaaccatt
     6301 cgatatgccg gacggagtga cgaaagtgac gatcgatccg gaaactttgg cgctggcaac
     6361 tccggaatgt cctacgtcac gcgaagaagt ttatattcat ggcacggaac ccacggaatt
     6421 ttgtcccttg cacggtgggc atatggcgaa cgatacggcg ccgggctcgt ggctttcgca
     6481 tgtttttggc ggggacaaaa acaaaaatgg agaagcggct cctcccgcgc cgaactctca
     6541 tccttcttcc accgcgccgg ctgctgccga gaatcaacag ccggaagagg agaacaaaaa
     6601 aggcgtcttg agacgaattt ttggtatctt tggaggcaag aaggacgccg acaaatcaca
     6661 atctggtcca caagattagc aaagcaacgc ggcgtccttc ggagatattc accatgaaaa
     6721 tgggatctcc atctcccagg atatccgttg ggctactttt ttttgtccta tccacgggtc
     6781 tcggcgcgca tccgtccgta aagaatcaag ccccgcaatc ttctcaatcg caatccaata
     6841 gcggcacgac tccgactctt tcgccaaaag aaatgcaggt ggaaagagcg aaagtacaaa
     6901 tggcggaaaa gcgtttcgaa gcggccatcc agacctatca ggatttgtta aaagccgatc
     6961 caaaaaacgc aagttacatg aacatgattg gaatcgcgta tttggatctg ggcaggaatg
     7021 acttcgctaa aaaatatttt ctccgcgcat ccaaagccga caagaaaaat tctaacgctg
     7081 taaataatct gggcatggtg tattaccacc agaaggattt ccgtcgcgca atccgtgaat
     7141 atcaacgtgc gattaccatc gatccggggc aggcaggtcc gcacgcgaat ctcggttttg
     7201 cctactacaa ttccaataag tttcccgagg ccgcgattga atttcaaaaa gcgctggaaa
     7261 tcgaaccgtc catttttgag cggaacgata aagtgggaag ctcggtgcag gaccgctcgg
     7321 tttccaatca cggattgttt ttcttcacca tggctcgcgt gtatgcgcag aaaaaagacg
     7381 cggagcattg cgctgaatat ttgcgcaaat cattggatga gggatataag gatgtgggca
     7441 aagccgcgac ggacccggct ttcaaggacg tggttgacga tcccgccatc caggccgttc
     7501 ttgtactcat tgcgaaaccc gactctaaag cggcgcccgc gcagcccggc gcataagttt
     7561 cctgcactgc aaggcccacg taagtccgcg ctgctcagcc tctgctacaa tgtgaatacg
     7621 atgccccatc gaatttttcg tttagcgatt tttatttttt gcgcggccct ttgcagtgtc
     7681 gcgacagagg gtcgggctcg ccccgcccaa actagcggcg ctgctcgctc gacttcatcc
     7741 tctgaatccc gtgccgaaat gattttgatt tttccttttc aaaatgatag tcgcatggca
     7801 aatctggatt ggctgggcga aggattgtca gagctgaccg cggagcgttt tgaagatcgc
     7861 ggagtgaatg tgctctcccg ggaagatcgg ctggcgacgc tggagcgcat gggattgccg
     7921 gattccgcgc gcttttcgca tgcaacgatc gtaaaaattg ccaccgaagc cgatgccgac
     7981 gctgtaattt ttggaaggtt tcagtatgac ggccaaaagg ccactctcga agcgcgcgtt
     8041 ttgcggatca gtcctccttc gctctctccg gccttcaccg agaccggctc aatggaagat
     8101 ttgctgcgtg cccacgcccg cctgacctgg aaagttttgt gtgccgtaaa taaaaaaaat
     8161 tgcccgttgc aaggtgcgag tcaggacgaa tcgagtttct cggagccgcc gccgtctttg
     8221 cgcccggaag ctctcgaaaa tttcattcgc ggagtgaccg gatcgcagga tgaagagcgc
     8281 cttcgcctgc tgcgtgaagc agcgcggctt gagccgaatt gggaccgccc ggcattcgag
     8341 ttgggtaaaa tttatttcaa gcggcacgat tgcgactccg cgctggtgtg gtattcgcgc
     8401 gttcctccca atcgcccgga tggacctgaa gcaagttttg cgactggcgt ctgccacctg
     8461 gcgcgcaatg acgccacgcg cgcggaagcg acgttttcag gtttgttgga acgcacgcgc
     8521 agaaccggtg aaaaagactg gcttccggaa ttgccggaaa tgcgtaacaa cctcggcgtg
     8581 tcccgcttgc aaatcggaaa gtggaatgag gcggaaacgg aattcgagcg cgcctccgcg
     8641 ctggatcccg aggaagccga ttactggatt aacatagcga ttgctaaact gataggaaag
     8701 caggcgcctg ccgccgtgac cgctctcgat catgctcgaa aaattgctcc cgacgacaag
     8761 gaagcgagag tgctgtccat cgcaactttg gattccctgg ggcgaagcac ggaagcttca
     8821 gccatgaggg ccgaagttac agaaaaaggc gataagcaag tgatgccgaa ccttcaggac
     8881 ggtgttgcgc tcgccagaat ggcgcgcgtt tccagaagtt ttgatcgaac acttctgcgc
     8941 cctggtggcg aagctcccga gggacagtcg ccggcaggga aggggcctcg cacgtcgaac
     9001 gatagcggag ttcgcccatg ataccaagcg gcagtggccg ccccacggat tttttccgtt
     9061 tacggaatgc tatagctatc gcaatttttt cacttgccgt ttcgctatct gtgtcactcg
     9121 tcctgcgagc acaagcgccc ccaccaaaaa attcttcctc acccggacaa tcacaaatcg
     9181 ttgagttgcg aatcggggat gaagtcgaac cggtgatggc ggagtatgtc gacagcggca
     9241 tcgaggaagc tgcccggcgc catgcaagtc tcgtcctgat cactatggat actccgggag
     9301 gactttcgac ctccatggag gacatcattc acaacattct cgtttctccc gtgcccgtgg
     9361 ccatctatat ttcgccagcg ggatcgcgtg gagcttcggc gggatttttt attttactct
     9421 ccgccgacat cgctgccatg gcgccgggaa ctcacacggg cgcagcttcg ccactgctgg
     9481 cgatcggcgg atttccggtg caagtggatg aaacactgaa gaagaaaata ttgaacgatg
     9541 ccacggcttt tctccgcagc tactccgcga agcgcggaag aaacgtggag cttgcggagt
     9601 cagctgtagt ggatggaaaa gcgtggacgg aaacggaagc cctcgacggc cacctcatcg
     9661 atttggttgc gaattcgcaa gacgatttgc tggcaaagtt ggatggccgc acgatcaagc
     9721 gcttcgatgg ttcgacagtg aagcttgaat tacacaatcc agtgtatgtt ccattcacaa
     9781 tgtcttttcg ccagcaattt ctttcgcgga tcgttcaacc cgacgcattt ttcattcttt
     9841 taattgttgg agtgcttggg ctctacgttg aattcaccca tccgggaatg gttgcgcccg
     9901 gagtggtcgg cggaattgcg ctggtgctgg cgctgtatgc gatgcatatt ctgccggtgg
     9961 cgcccgccgg gatcgtattg attattttgg cgttgggatt atttattgtg gaggcgaagt
    10021 acaccagcca cggagtgctg gcaatcggcg ggattgtctc catgctgctg ggcgcgttga
    10081 tgctggtgcg ctcgccgctg acgcatgcag gtgtgagcct cggtgtggcg ctgggcgtaa
    10141 cgctgccgtt tgcgatcatt gtaatttttc tgatgcggct ggtgctgaaa tcgcggtcgt
    10201 ggaagccgtc catggggcgc gaacaatttg tgggaaacgt tgcggaagtc accagttcta
    10261 tcgtgaagac agcagaggaa ggaatgtttg agggcatggt gcggctcaat ggggcgttgt
    10321 ggcgcgcggt tgcccgagaa gcgattcccg gtggatcgca cgttcgtgtg gtaagtttcg
    10381 aggggctgac tttgcacgtt gttccggttg agcactccgc tttggcaaaa taaattgctc
    10441 tagcacacac tggaaagtat ttaacgatgg gtcgggcagc aggcgaggag gaaatatgga
    10501 tctttcgagt ttttcactct acggcgttat gatcgcgctc ttcatcggtt ttatctggtt
    10561 ctggaattcg gtgaatgtgc taaaggagtg ggagcgcggc gtggtattgc gcctggggcg
    10621 catgatgccg aacgccaaag gtcccggctt gtgcctcgtt ttctatccga tcgaaacgct
    10681 gactcgtgtc tctttgcgaa tcgaaacgct ggacgttcct tcgcaagaca tcattacgcg
    10741 tgataacgta tcggtgaaag taaatgcggt tgcttatttc gcggtggtcg atgcaaacct
    10801 cgcagtctcg cgcgtgcaga attacatgta cgcgacttcg cagaaggcgc agaccacgct
    10861 gcgcagcatc gtcggacaat ttgagctgga tgagattctc gccgaacgcg aaaaagtaaa
    10921 tagtaaattg caggtgattc ttgaccagga caccgagccc tggggtatca aggtaacgaa
    10981 ggtggaagta aagcaggtcg atattccgga gcagatgcag cgtgccatcg ggcgccaggc
    11041 ggaggcggag cgcgaacggc gcgcaaaagt gattcacgcg gagggcgaag cgcaagcttc
    11101 cgcgaaattg gccgaggctg cgaaagtatt ggaaacgcag cccgctgcga ttcaacttcg
    11161 atacctgcag actctgactg aaatcggcgt cgaaaaaaac accacgattg tgtttccgct
    11221 cccaatcgat atcatgaacg agtgggtcaa ggcgatcggt tccagaaaac aatagatggc
    11281 gtcaaatact aaaaagagcg gcagcgattt tgttctcgag agcaggcacc tggtcggatt
    11341 gtttctgctt ctcgtcgtta tttttggtgt ggtttttacg ctcggttatt tgatgggccg
    11401 aagccagggc gattccagaa gtcgtaccgt cattgccgct ccggccgaac ccagtacgtc
    11461 tgccgctgtt cctccatcct cgaaatccaa ggccaagccc gcaccgccgc cggacgatac
    11521 gcagacgcaa aaaacaaatt ccgattggga tttttatcat tccgcggatc cgcaggcgaa
    11581 ctccgatcgt ttgcaaccgc cttcaaaggc tgcggctcct cccgccgcga tgtccaaacc
    11641 cgctccatct ggaaaggccg caaagcaggg gaaagcgccg cccggattgg gcggtccgtt
    11701 gattccgaaa gggacgatca tgctgcaggt ggcggcggtg ctgcatcagg acgatgcgct
    11761 ggccctcgcc caggctctgc agaagaaaaa atttccagcg atcgtgattc ctcccaacgc
    11821 ggacaaatat taccgcgtcc aggtcgggcc ctacgccgac aaccaggcgg cagccaatgc
    11881 ccgcacggat ttggaagcca atggattcaa gtccatcacc aaacgctaga aacctggcaa
    11941 tactaattct agttgtcctc tcggaagcac tgtaattaat aaaatgaaaa gttcgacagc
    12001 gaattgtggc tgtccctcat cgggcggtct acaatgccgt ggctatgaac tcgtcgagga
    12061 ctgtgcggtt gttgcttgcg cttgtttcgg gcgttgcatt ggcgctggcg tacccgctat
    12121 ttaatctccc aatcctggga tggatcgcgc ctgccgtatt aattatcgct gtaatcggcg
    12181 aacggccgcg atttgcaatt gttttgggat ttgtgcaggg cgcggtgtat ttcgggctga
    12241 gcttgccgtg gttttataac gtcatgcggc aatacggacc gctgcccgtt gcgcaggcgg
    12301 caggcgtctt cctgttagtc gttcttgttt tgtctatctt tcatgctgtt tttgccgcgg
    12361 caattgcgtg gctcggaaaa gcttcgtcca aacgcgcctg cctggccgcg ccatttcttt
    12421 gggtggcgct ggaattcggc acgatgtaca tgccggcgat cggctttccg tggaatttac
    12481 ttggctatgt ggcggcggga aatttagcat ttgtacaaat aacggcaatc acgggaattt
    12541 tcggcctttc gtttctggtc gcggcctata gcagcctttt ggcatgggct gttctgcagt
    12601 tctcacgata taaaaaaaca gaatggaaat tgctgggggg agcaacagcc acactcattg
    12661 tcattgccct tgcgggaccg caattcgtgc cgaaagcttc cgcggaccac gtcgctcatc
    12721 tggtgcagac aaattttccc gt
//
LOCUS       DASZMB010000238         4809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1369918, whole genome shotgun sequence.
ACCESSION   DASZMB010000238 DASZMB010000000
VERSION     DASZMB010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369918"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /gene="ribB"
                     /locus_tag="VG272_11240"
     CDS             complement(<1..461)
                     /gene="ribB"
                     /locus_tag="VG272_11240"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HWF14302.1"
                     /translation="MSAEPIPFATVEEALEEIRQGRQIVLVDDEDRENEGDLTMAAEK
                     ITPDAVNFMAKHGRGLVCLALTEERCDELALPLMSPVNTSNYGTAFTESIDARRGTTT
                     GISAADRAITILAAIDPASRPTDLARPGHIFPLRARNGGVMVRAGQTEASV"
     gene            complement(768..1283)
                     /locus_tag="VG272_11245"
     CDS             complement(768..1283)
                     /locus_tag="VG272_11245"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWF14303.1"
                     /translation="MPIAQMLLPEFDHEMANTRKMLERVPDGKFAFKPHEKSMTLGRL
                     AAHTAEIPSYATGTLRVERMDFTGEEKPFSPATRKEMLDAFDKYVVEARELLSKATDE
                     DLMKTWTLTFKGQQIFSMPRAAVLRTMVMSHLVHHRAQLGVYLRMNNVEIPGMYGPSA
                     DEKNMFNAQNA"
     gene            complement(1503..3263)
                     /locus_tag="VG272_11250"
     CDS             complement(1503..3263)
                     /locus_tag="VG272_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14304.1"
                     /translation="MSEERGAWWDRAAALFLFISTSAVVIWQNSRLAVLWDLSYILEN
                     SYRISRGDFPYRDFPFPYAPGTFLVQSLLIKLTGRVFFHHVLYCAMVGGLATVVTWRI
                     LTYMLRESMPSFRLVAFLLALPLTVLGIYCVFPHPFYDSDCTFIILLCVLLFLKLEQT
                     NFPPRQTFFAGVLFVLPIFVKQNTGLAFAASAQWATLILILYAVWRRQRVAGYVWLLA
                     GMTAGFSAALLLLHFTVGLTNYWHWTVQFAASRRLPGFAEMLTPYENPLLLLWIAALA
                     AGSLLLWYGNHRGNRMLSSLSIALLCAPFVWAVLYLVMDDDPAERAERLLALWPVILI
                     ASLLFALWNLKKGPTVARLLPFILIGTVQGAFLSQQLWGSTYAIWPLLMILLAGILAE
                     LFSLGNKNSLREIEWLAAIATLSMFIAGTFYVVSHERLDYADLSSGELAHSTLPALRG
                     LSMRGPWLQQFDELVRFSDREIPRDQGILMIPGEDLFYYATGRRPQFPVLMFDHTVNP
                     LSPQEILDVSRQRNICWLIFKKRLQLNADPVEDKARLLDLLRGDFSLVQSLANYDVYR
                     KFAPRGCEEKNSLAPVSLGN"
     gene            complement(3498..4400)
                     /locus_tag="VG272_11255"
     CDS             complement(3498..4400)
                     /locus_tag="VG272_11255"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWF14305.1"
                     /translation="MSIRPVKRLVKSKPTREGAGVHLRRAFGFGNTSEYDPFLLLDDF
                     RNDVPEDYLAGFPWHPHRGIETITYVLAGTVDHGDSLGNQGSIAPGDVQWMTAGSGII
                     HQEMPKGDQAGRMHGFQLWSNLPGSLKMTAPRYQGVEAKDIPNITDDDGTHVRLVCGK
                     FWGKSGPVDGVAADPIYLDVSVPPGHRKTLPVETTRHAFAYVFAGSGKFCNASEPLAV
                     PTESVGWADTNPPTAADNRSLVLFDQGDEVAVQAGEEGVRFLLVSGKPLEEPVAWYGP
                     IVMNTQSQLQKAFQELQEGTFLKK"
     gene            complement(4462..4671)
                     /locus_tag="VG272_11260"
     CDS             complement(4462..4671)
                     /locus_tag="VG272_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14306.1"
                     /translation="MGSKSKLKDAAVKIGSVVGKVDGTAHKAARKTAEAIKVARRELD
                     DVSKQVEALKKQLAKSSKRLQDTLS"
BASE COUNT         1124 a         1305 c         1319 g         1061 t
ORIGIN      
        1 tccacggagg cttccgtttg ccccgcacgc accattacgc cgccatttcg cgcgcgcaag
       61 gggaaaatgt ggccgggacg cgccaggtcc gtgggacgag acgccgggtc aatggccgcg
      121 agaatcgtaa tcgcccgatc ggcggcactg atcccggtgg tagtgccgcg ccgggcgtca
      181 atcgattcgg taaaggccgt gccgtagttt gaggtgttta cgggggacat cagcggcagc
      241 gccagttcgt cgcagcgctc ctccgtcaag gccagacaaa ccaagccacg cccgtgcttg
      301 gccatgaagt tgaccgcatc gggagtaatt ttttccgctg ccatcgtgag gtcgccctca
      361 ttttcgcggt cctcgtcgtc caccagtacg atttgccgcc cctggcggat ttcttcgagg
      421 gcttcttcca cggtggcaaa cggaatgggt tcggcggaca tagctgcttc gcgactcctg
      481 cgtgttgacc tataaattta ggtggctttc gtaactatac cgaatccggc atggggcggc
      541 aagattgggc tgttgagagt ggtgcgtcag attgaattgc acgcacaaaa agactgcccg
      601 cccgaaaaga gggcggcggc tacgaaaaac aatgtttgca acgctttatg tcctcgtagt
      661 ggcctgtccc tcccgttcac ggagaacgtc tctgtgaacc gcgtttttgg gacggacagg
      721 ccattttttc cgtgcaaatc gacggcaggt tcctgtgcgc gcaattgtta tgcgttttgt
      781 gcgttgaaca tattcttctc atccgccgag gggccgtaca tcccggggat ttcgacgtta
      841 ttcatgcgca ggtatacgcc aagctgggcg cggtggtgaa cgagatggct catcaccatg
      901 gtgcgcaaca cggcagcgcg cggcatggag aaaatctgtt gccccttaaa ggtcagcgtc
      961 caggttttca tcaggtcttc atcagtcgct ttcgaaagta attcgcgggc ttccaccacg
     1021 tatttgtcga acgcatcgag catttccttg cgggtcgccg gcgagaaggg tttctcttcg
     1081 ccggtaaaat ccattcgttc cacccggaga gttcctgtcg catacgacgg gatttcggct
     1141 gtgtgcgcgg ccaggcgtcc aagagtcatg gatttttcgt gcggcttgaa tgcgaatttt
     1201 ccatcgggga cgcgttccag catcttccgt gtattggcca tctcatgatc gaattcgggt
     1261 aaaagcattt gagcaattgg cataggttcc tcctaagaat ttggcgttcg gggggcgggg
     1321 cagagcatac cactggagta tgggcgaata caaggcgaag attgtgcgta aacggtgaga
     1381 cggtggaaat cgtcgtcacg gtgctccttc tttccgaaag gtgctggaca gaaatgcctt
     1441 tacgataatc gccgcattgg ttttcgtaga ggccgctcga attatgggcg ggcattctta
     1501 gttcaattac ccaagctcac cggagcaaga gagttttttt cctcgcagcc tcgtggagcg
     1561 aatttccggt aaacgtcgta attagctaag ctttgaacca gcgaaaaatc gccgcgcagc
     1621 agatcaagca accgcgcttt gtcctccacc gggtccgcgt tcaattgcag acgtttttta
     1681 aagatcaacc agcagatatt ccgctgtctg ctcacgtcta aaatttcttg cggactgaga
     1741 ggattcaccg tgtgatcgaa catcagcacc gggaattgcg gccttctacc cgtcgcgtaa
     1801 tagaaaagat cttcgcccgg aatcatgagg attccctggt cgcggggaat ttcgcgatcg
     1861 gagaagcgca cgagttcatc gaattgctgg agccatggtc cgcgcatcga gagtcctcta
     1921 agcgcaggca aagttgaatg cgccagttcg ccggaagaaa ggtctgcata atcgaggcgc
     1981 tcgtggctca ccacatagaa tgtgcccgcg atgaacattg acagcgtggc gatcgcggcg
     2041 agccattcga tttcccgcaa tgaattttta tttcccaggc tgaacagttc cgccaaaatt
     2101 cccgcgagca aaatcatcag cagaggccag atggcatagg tagagcccca gagctgctgc
     2161 gacagaaatg caccctgcac agttccgatc aaaataaaag gcaggagtcg cgcgaccgtc
     2221 ggaccttttt tcagattcca aagcgcgaag agcaaggaag cgatcaggat cacgggccat
     2281 agtgcgagca aacgttccgc gcgctcggca ggatcgtcgt ccatgaccaa ataaagaacc
     2341 gcccaaacga aaggagcgca caggagcgcg atggacagcg aggaaagcat tcgattaccg
     2401 cgatggtttc cgtaccacag caataacgaa cccgctgcaa gcgccgcaat ccataggagc
     2461 agaagcggat tttcgtacgg cgtaagcatt tcggcgaagc cgggcaatcg tcgtgacgcg
     2521 gcaaattgaa cggtccagtg ccaataattt gtcagtccca ctgtgaagtg aagcaagaga
     2581 agtgccgcgc tgaaacctgc cgtcattccc gccaacagcc acacgtagcc ggccactcgt
     2641 tgccggcgcc ataccgcgta caaaataaga atgagcgtgg cccattgagc gcttgccgca
     2701 aatgcgaggc ccgtgttctg cttcacaaaa atcggcagga caaaaaggac acccgcaaaa
     2761 aaggtttgcc gcggcggaaa gtttgtttgt tccaacttta gaaagagcag aacgcacagc
     2821 aggatgatga atgtgcaatc cgaatcgtaa aaaggatgtg gaaaaacgca gtaaataccc
     2881 aagaccgtaa gcggcagagc aagcagaaac gccacgagac gaaaactggg catggattcg
     2941 cgcaacatgt aggtcaaaat tcgccacgtg acaaccgttg ccagcccgcc aaccatcgcg
     3001 caatacaata cgtgatgaaa aaacacgcgg cccgtgagct tgatcagtaa tgactgcacc
     3061 agaaaagttc ccggcgcata aggaaaaggg aagtcgcgat aaggaaaatc gccgcgagaa
     3121 attcgatagg aattttccag aatgtagctg aggtcccaga gaacagcgag ccgcgaattt
     3181 tgccaaatga caacagccga agtgctgata aataaaaata atgcggcagc cctgtcccac
     3241 cacgccccgc gctcttcgct catggtttta gtgggatagg cttcagcccg tctgcttttg
     3301 aatttttcac gtcacgcgca aatttaacac accgaaaaag tattgagcga atcatgagca
     3361 accgtgaagc gccgtctcac caacagattt ggaagaatcg cgggtgagca cacacatatt
     3421 ttgtccatgt ggtgcccgcc tgaaggcggg cgccactgtg caaaaactgg gttgccgcaa
     3481 agattctgaa acctggccta ctttttcagg aatgtgcctt cttgcaattc ctgaaatgct
     3541 ttttgcagct gcgattgcgt gttcatcacg atgggaccgt accaggcgac cggctcttcg
     3601 agcggctttc ctgaaaccag caggaagcgc acgccttcct cgcccgcctg aacggcaact
     3661 tcgtcgcctt gatcgaagag cacgagcgag cgattgtccg cggccgttgg aggattggtg
     3721 tcagcccagc ctacggattc cgttggcacg gcaagcggtt ccgaagcatt gcaaaatttt
     3781 cccgagcctg caaagacata agcgaatgcg tggcgcgtgg tttccacggg caaagttttt
     3841 ctgtggccgg gcggcacgga tacgtcaagg taaatgggat cggcggcgac gccgtcaacg
     3901 ggtccggact ttccccagaa ttttccgcac acgagccgca cgtgcgtgcc atcgtcatcc
     3961 gtgatgttcg ggatgtcttt cgcttccacg ccctgatagc ggggcgcggt cattttcagc
     4021 gagcccggca aattggacca gagttgaaag ccatgcatgc gcccggcctg atcgccctta
     4081 ggcatttcct ggtgaataat tccgctgccc gccgtcatcc actggacgtc gcccggcgcg
     4141 atcgaccctt gattacccaa gctgtcgccg tggtccacgg ttccggccag cacgtaggtg
     4201 atggtttcaa ttccgcgatg cgggtgccaa ggaaaaccgg cgagataatc ttcaggaacg
     4261 tcattgcgaa aatcgtcgag aagcagaaag ggatcgtatt ccgacgtatt gccaaaaccg
     4321 aacgcgcgcc tcaaatgcac gcccgcacct tcgcgagtgg gtttggattt gacgagacgt
     4381 tttaccgggc ggatggacat gctagcctcc tgcaatggcc agtctactcc atggcgcgca
     4441 gcgatgacaa tttcggcgca attaactcag agtatcctga aggcgtttgg agctttttgc
     4501 caattgcttt tttagagctt ccacctgttt ggatacgtcg tcgagttcgc gtctcgccac
     4561 tttaatcgct tccgccgttt ttctggccgc tttgtgtgcg gtcccatcca ccttgccaac
     4621 gacagaaccg attttcactg cggcatcttt tagtttgctc ttgctgccca tgtcattccc
     4681 tccacttgaa gttacgatgg cagactaccc gcgccaatag caaggccaaa tccggattca
     4741 atatttctac cgtttttccg cccccccaaa aatcatattt tgggtaagcc tactatccgg
     4801 tagtgcctc
//
LOCUS       DASZMB010000239         4960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2706817, whole genome shotgun sequence.
ACCESSION   DASZMB010000239 DASZMB010000000
VERSION     DASZMB010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2706817"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VG272_11265"
     CDS             <1..852
                     /locus_tag="VG272_11265"
                     /inference="COORDINATES: protein motif:HMM:NF042697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HWF14307.1"
                     /translation="NDTLSPVDLGSYASASGERVLIVVGEMLLDITAPAKQMLFDVPV
                     PAKQARPLDADLKQLTDVLAKKADRTSYPFIGEHLPEKGRVRNSERYVIGPLGLAHFV
                     PLGTDDWMGYDNSAESILAHYTVNGKDETLIVSSYPTQQVAAAKFAKMLRRFTFDPPG
                     EDAPIGPNVLFGKRVSSFIAVVAGAPSKQAANKVLDQVGYESNVTWDEPKQTLTEPSI
                     NNMIVEAFLGTGAIMGFAVAAGIGFGGIRLLVKIFLPNKVFDREKQIEILQLGISSKP
                     IKAKDFY"
     gene            complement(1129..1458)
                     /locus_tag="VG272_11270"
     CDS             complement(1129..1458)
                     /locus_tag="VG272_11270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HWF14308.1"
                     /translation="MSQPIVPQKTYIFVFLALIALTILTTAVAFVDLGAFNTVVALTI
                     ACCKMALVMLFFMGVRHSGGLIRIIVIAGFFWFALLVAFTMTDYHTRGWNPVPQAWSS
                     GAPPTHP"
     gene            complement(1458..2141)
                     /locus_tag="VG272_11275"
     CDS             complement(1458..2141)
                     /locus_tag="VG272_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HWF14309.1"
                     /translation="MTDAEAHAEKGIAHQFEDLDQQHEADTLGIWVFLITEIMFFGGV
                     FAAYAIYRWLYFAAFDGGSHILDIRLGAINTIVLLGSSLTMVLAVRSAQTGNRKATVW
                     YLIATMILGSVFLGVKAYEYNQKFVEHVVPSLDWAPEGEALKLLAPGGLQHAQIYFIF
                     YFAMTGLHAIHMIIGMGLLLWLVLRARKDDFTPHYFAPVEVVGLYWHFVDIVWIFLFP
                     LLYLIGGRY"
     gene            complement(2134..3762)
                     /gene="ctaD"
                     /locus_tag="VG272_11280"
     CDS             complement(2134..3762)
                     /gene="ctaD"
                     /locus_tag="VG272_11280"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HWF14310.1"
                     /translation="MSTATLEPRVHTPRNYLNADYGWKSWLFTLDHKRIALLYLVSVT
                     FFFLVGGSFAVLMRIHLTEPQGALVQPETYNKLFTLHGVVMIFFFLIPSIPAVLGNFL
                     VPLMVGARDLAFPKLNLLSWYIYIVGGLFTLYSAIAGGVDTGWTFYTPYSSTYSNTQV
                     TAVAIGVFVTGFSSILTGLNFIVTIHKMRAPGLTWFRMPLFLWAMYATSLIMILGTPV
                     LAITIFLLALEHLFHVGIFSPALGGDPLLFQHMFWFYSHPAVYIMILPAMGVVSEIIA
                     CFSRKRIFGYTIVAFSSVAIAVLGFLVWGHHMFVTGQSVYAGLIFSLLSFLVAIPSAI
                     KVFNWTATLYKGSISYEAPMLYAMGFIGLFTMGGLTGLFLASLATDVHLNGTYFIVAH
                     FHYIMVGGAVMGYLGGIHFWWPKISGRKYPNMWAGIAAVIIFIGFNLTFFPQFILGYL
                     GMPRRYAFYPAEFQVLNVMSSAGATVLGVGYLIPLIYLIWSMRYGPVAEANPWGATGL
                     EWTTPSPPPTENFTEIPVVTEEAYAYGPKEGSGD"
     gene            complement(3759..4718)
                     /gene="coxB"
                     /locus_tag="VG272_11285"
     CDS             complement(3759..4718)
                     /gene="coxB"
                     /locus_tag="VG272_11285"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HWF14311.1"
                     /translation="MQSLPFQPEQASSIAKSVDELYLFLTLITLFFTALIFGMIFYFM
                     IRYRRRSPDERPKPIEGNVPLEILWTAVPTLIVALIFVWSSMLYFKNAEAPKGSMEIF
                     VTGKQWMWKVEHPEGQREINEVHVPLGRPVKLTMTSEDVIHDFFVPAFRVKKDVLPGR
                     YTSLWFTPTKVGTFHLFCAQYCGAYHAGMMGAIVVQEPDDYERWLAGGAPGESMEQAG
                     EKVFQANGCITCHTPDGAGLGPSLLGVYGQPVKLVGGETVTADDAYIRESILQPKAKV
                     VLGYTPIMPTFQGQLTEEQLNNLIAYIRALGKSQPKSKEEGKQ"
     gene            complement(4746..>4960)
                     /locus_tag="VG272_11290"
     CDS             complement(4746..>4960)
                     /locus_tag="VG272_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14312.1"
                     /translation="GKIGTVTDHAMLYCYQYDPMTGKYGIVIINVLRIAGGLTVLALG
                     LFMTIMFLRERKRPTGVPPATDARAR"
BASE COUNT         1264 a         1388 c         1332 g          976 t
ORIGIN      
        1 aacgacacgc tctctcccgt tgaccttgga tcgtacgcca gcgcttctgg cgagcgagtg
       61 ctgatcgtag tgggcgaaat gctcttggac atcacggccc cagcgaagca aatgctcttt
      121 gacgttccgg ttccggctaa acaggcgcgc cccctggacg cggatttaaa gcaattgacc
      181 gacgtgctgg cgaaaaaagc ggaccgcacg tcatatccat ttatcggcga acatctgccg
      241 gaaaaaggcc gcgtgcgtaa ttcggagcgc tacgtgatcg ggccgctggg cctcgcgcac
      301 ttcgtcccgc tcggcaccga tgactggatg ggctacgaca acagcgcgga aagcatcctg
      361 gcccactaca cggtgaacgg caaagacgaa acgctgatcg tcagctccta cccgacgcaa
      421 caagtggcgg cggccaaatt tgccaagatg ctgcgccgct tcacattcga tcctccggga
      481 gaggacgctc ccatcggccc caacgtttta tttggcaagc gcgtgagttc gtttatcgct
      541 gtggtggcgg gtgcgccttc gaagcaagcg gcaaacaaag tactcgatca ggtcgggtat
      601 gagagcaacg tcacatggga cgagccgaag caaacgctga ccgaaccgag catcaacaac
      661 atgatcgtcg aagctttcct tggaacgggg gcgatcatgg gtttcgcagt cgcagcgggg
      721 attggctttg gtggaatacg cctgctggta aaaatcttcc tgcccaacaa agtgttcgac
      781 cgcgaaaagc aaattgagat tttacaattg ggaatttcca gcaaaccaat caaggcaaag
      841 gacttctact agcagcgcgt cccctgttga aaataaggct agcaaatcag cttctcgcct
      901 gaccttacaa gtacaagcta ttgtaaacaa aacaacttat agtcatttag attcaatgct
      961 ttctccgctt gactttgttc tttggactcg aatagacttc gcgcgaggac atagagttct
     1021 agtgccttcc gcgcataggc acgggactat tctcccctct tgaaaacccg tccgtgaaaa
     1081 cggacgggtt ttctgtttcc agggtcaatt ttaaattaat ttacgggctt atggatgggt
     1141 gggcggcgcg ccggacgacc acgcttgtgg gacagggttc catccacgcg tgtgatagtc
     1201 cgtcatcgtg aaggcgacca gtagcgcgaa ccagaaaaat ccggcgatta cgataatgcg
     1261 gatgagcccg ccgctatgcc gtacgcccat gaagaacagc atgaccagcg ccatcttgca
     1321 gcaagcaatc gttagtgcaa cgaccgtgtt aaatgcgccg agatccacaa acgcaacagc
     1381 cgtggtcagg atcgtaagcg cgatcaatgc aagaaaaaca aagatatagg ttttttgcgg
     1441 cacaatcggt tgagacatta gtagcgtccg cctatcaggt aaagcaacgg aaatagaaaa
     1501 atccacacga tatcgacgaa gtgccagtag aggccgacca cctccacggg cgcaaaatag
     1561 tgcggcgtaa agtcgtcttt gcgcgcacgc aaaaccagcc agagcagcag gcccatgccg
     1621 ataatcatgt gaatcgcgtg taatccggtc atcgcgaaat aaaatatgaa ataaatctgc
     1681 gcgtgctgca acccgccggg ggcaagaagc ttaagcgcct cgccttcggg cgcccagtcg
     1741 agcgacggaa cgacgtgttc cacaaatttc tgattgtact cgtacgcttt cacgccaaga
     1801 aagacgctgc cgagaatcat ggttgcgatc agataccaaa ccgtggcctt gcgatttcct
     1861 gtttgcgcag aacgtacggc gagcaccatg gtcaaactac taccaagaag gacgatcgta
     1921 ttgatcgccc ccaggcgaat gtccagtatg tggctgccgc cgtcaaatgc cgcaaaataa
     1981 agccagcgat aaatcgcata cgcggcgaac acgccgccaa aaaacatgat ctccgtgatc
     2041 aggaaaaccc agattccgag tgtgtcagct tcgtgctgct gatcgaggtc ctcgaactgg
     2101 tgcgctattc ccttttctgc gtgcgcttcg gcgtcagtca ccggagccct ccttaggacc
     2161 gtatgcatac gcctcttcgg tcaccacagg aatttccgta aaattttccg tcggcggagg
     2221 agatggcgtc gtccattcca agcctgtggc gccccaggga ttcgcttccg cgaccgggcc
     2281 gtaacgcatc gaccagatca ggtaaatcaa aggaatcagg tagccgacgc ccaacaccgt
     2341 ggcgcccgcc gaagacatca cgttcagcac ttgaaactcc gcgggataaa acgcgtagcg
     2401 gcgcggcata cccagatagc cgagaatgaa ctgcgggaaa aaagtcaggt tgaaaccgat
     2461 gaagatgatg accgcggcaa tgccggccca catgttcgga tatttgcggc cggaaatttt
     2521 gggccaccag aaatgaattc cgccgaggta tcccatcacc gcgccgccga ccatgatgta
     2581 gtgaaagtgc gcaacgataa aatacgtgcc gttcaaatgc acgtccgtcg cgagtgaggc
     2641 gagaaacaat ccggtaaggc cgcccatcgt gaagaggcca ataaaaccca tcgcgtagag
     2701 catcggcgct tcgtaggaaa tcgatccctt gtacaacgtc gcggtccagt taaaaacttt
     2761 gatggcggat ggaatcgcga cgaggaaact gagcagcgag aaaatcagcc ccgcgtatac
     2821 cgattgtcca gtgacgaaca tgtgatggcc ccagacgaga aaaccaagaa ccgcaatggc
     2881 cacgctggaa aacgccacga tcgtgtaacc gaaaattctt ttgcgcgaga aacacgcgat
     2941 gatctcgctc accacgccca tagccggcaa aatcatgatg tacacggccg gatgcgagta
     3001 gaaccagaac atgtgttgaa acaagagcgg atcgccaccg agcgccgggc tgaaaatgcc
     3061 cacgtgaaac agatgctcca gcgcgagcag gaaaatcgtg atggccagga ccggcgtgcc
     3121 gagaatcatg atcaggctgg tggcgtacat cgcccacagg aagagcggca tgcggaacca
     3181 cgtgaggccg ggagcgcgca tcttgtgaat cgttacgatg aaattcaggc ccgtgagaat
     3241 ggaagaaaac ccggtgacga acacgccaat cgccacggcc gtgacttgcg tgttcgagta
     3301 tgtgctgctg tagggcgtat aaaatgtcca gcccgtatcc acgccgccgg cgattgccga
     3361 atataacgtg aacaacccgc ctacgatgta aatgtaccag ctcagtagat tgagtttcgg
     3421 aaacgccaaa tcgcgcgcgc caaccatcag aggcaccagg aaattgccga gcaccgcggg
     3481 aatcgacgga atcaggaaaa agaaaatcat gaccacgccg tgcagcgtga acagtttgtt
     3541 gtaggtttcc ggttgcacga gcgcgccctg cggctcagtg aggtgaatgc gcatcagcac
     3601 tgcgaacgag ccgccgacga ggaaaaagaa tgtgaccgaa accaggtaca acagcgcgat
     3661 gcgcttgtgg tccagcgtaa acagccagga tttccagcca tagtccgcgt tcaaataatt
     3721 gcgcggagtg tgcacgcgtg gttccagcgt ggccgtactc attgcttccc ctcttccttt
     3781 gatttcggct gcgatttccc gagcgcgcga atataggcaa tcagattatt cagctgctct
     3841 tccgtcaact gaccttgaaa ggtcggcatg attggagtgt aaccaagtac gaccttggcc
     3901 ttgggctgca aaatcgactc gcgaatgtat gcgtcgtccg cggtgaccgt ttccccgccc
     3961 accagtttaa ccggttgtcc gtacacaccc agaagcgacg gcccaagacc cgcgccgtcc
     4021 ggtgtgtggc acgtgatgca gccattcgct tgaaaaactt tctcgcctgc ctgttccatc
     4081 gattcgcccg gcgcgccgcc cgcaagccaa cgttcatagt cgtccggttc ctggacgacg
     4141 atcgcgccca tcattccggc gtggtacgcg ccgcaatatt gcgcgcagaa cagatgaaaa
     4201 gttcccacct tcgtcggtgt aaaccacagc gaagtgtagc gcccgggcag aacgtctttc
     4261 ttcacgcgaa acgccggcac gaagaaatcg tggatcacat cttccgacgt catggtcaac
     4321 ttcacaggac gcccgagcgg cacgtgcact tcgttgatct cgcgctgccc ttccggatgc
     4381 tccactttcc acatccactg cttgccggta acaaaaattt ccatggagcc tttaggggct
     4441 tccgcgtttt tgaaatacag catggaactc cagacgaaga tcaacgcgac aatcagcgtc
     4501 ggcacggctg tccaaagaat ttccagcgga acgttgcctt caatgggctt cgggcgctcg
     4561 tccggcgatc gccggcgata gcgaatcatg aaataaaaaa tcatcccgaa aatcagcgcc
     4621 gtaaaaaaca gcgtaatgag ggtcaaaaac aggtacagct cgtcgaccga cttcgcgatg
     4681 gacgaagcct gttccggctg aaatggaaga ctctgcatca agatcgtatc ctcacgtctt
     4741 tatcctcacc gcgccctggc atcggtcgcc ggcggaactc cggtcggacg cttgcgttcg
     4801 cgaagaaaca taatggtcat gaataatccg agcgccagca ccgtcaggcc gcccgcaatg
     4861 cgcagaacgt tgatgatcac gatgccgtat ttcccggtca tcggatcata ctggtagcaa
     4921 taaagcatcg cgtgatctgt gaccgttccg attttgccct
//
LOCUS       DASZMB010000240         4512 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_232314, whole genome shotgun sequence.
ACCESSION   DASZMB010000240 DASZMB010000000
VERSION     DASZMB010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232314"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VG272_11295"
     CDS             <1..387
                     /locus_tag="VG272_11295"
                     /inference="COORDINATES: protein motif:HMM:NF024687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HWF14313.1"
                     /translation="NPIPGDEIVGYVTRGRGVAVHSNSCPNVQNLLYQVERRIDVEWG
                     GTTGSTYPVTLLIRAQDRPGMLADITAVISETGSNIRTLGSHPDNLHARIDLTLEISD
                     RKQLERIIANIRKISGVFGVERLYNV"
     gene            594..1172
                     /locus_tag="VG272_11300"
     CDS             594..1172
                     /locus_tag="VG272_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14314.1"
                     /translation="MLAAIAFLSSCKSAPKADTEETNQMAMWLDSVPQLKTLGISNAE
                     VNELRKAHEAGLTDPSSVVLIKMARDRHTPFTDGQSVADLLNAGSTEATVLQLARFNS
                     LGIWAGEASAMRLAGLSDKIILAVAERRSQGLPVLPGEKLGELKNTGASDATILDFVQ
                     KGLSEKSVNDYVAQRNRVAGHSFVYQRRSRKG"
     gene            1295..1909
                     /gene="def"
                     /locus_tag="VG272_11305"
     CDS             1295..1909
                     /gene="def"
                     /locus_tag="VG272_11305"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HWF14315.1"
                     /translation="MKIEIVQAGNPVLRQRARQLSVEEIRSRETQKLMEAMRTCMHEA
                     PGVGLAAPQIGLPIQLAVIEDREEYHKDVSEALLAERERRPVPFHAIINPTIQLIGEE
                     KAEFFEGCLSVAGYSALVSRSRVVRVTCLDEWGVAQTIEASGWYARILQHEIDHLNGT
                     LYIDRMRARSYTTTENLVEFWKGKSVSEIRAELEIGHPADATTE"
     gene            1966..2343
                     /locus_tag="VG272_11310"
     CDS             1966..2343
                     /locus_tag="VG272_11310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWF14316.1"
                     /translation="MIETVATDRGPKAIGPYSQAVRANGFVFLSGQIPLDPKTQQIVE
                     GDVAVQTERVMENLKGVVEAAGSSLHRVVKTTVFLADMNDFAAMNEVYARYFTAHAPA
                     RSTVEVARLPKNVRVEIDLIALL"
     gene            complement(2365..2754)
                     /locus_tag="VG272_11315"
     CDS             complement(2365..2754)
                     /locus_tag="VG272_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14317.1"
                     /translation="MSINSQHEYAVYLFEQGFFADAVTHLDEVIREEETAERWSDWAT
                     AQFALTHFPEAERGFRRALELNPDLPEAAVSYGAMLAGMSRWHEAIAMLDAVLPNLEP
                     EGRGAVDALAEQCRAKVSPLAQEAAAH"
     gene            2984..>4512
                     /locus_tag="VG272_11320"
     CDS             2984..>4512
                     /locus_tag="VG272_11320"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HWF14318.1"
                     /translation="MAISWWNNFDYALPVSNIPCIPEVHMTQARLLILEVTSHCNMHC
                     TFCPSDDLVRRKGNVSDEQARQLIRMASEVSPGVPIMFNVLGEPFLNKKLFEYIGLCE
                     ENKVPVVLITNITLLNEDRLRKLFTYSNVHLAMSLHTPTERAFASRGYKKIDNFRDYL
                     EMVCNTVEAKFRAGSKTTIEIYLASELIENLMQNDAGSRLWTMYDDPAEYKEGWDLCA
                     KRLTELSAKIARDYPEVFTEGMEKFRRDQKDLVKAGEIVFRAEDLPEWRLEGEMSGWL
                     CAPGVFVRRKGFGMWAYHEKFVQRHAEAARFVFHEERTEPFQCVGALTFGILSDGTYT
                     LCCQDVEGQMDIGNIADMEPRTAFESPRRQEIIDNCATSRVCRRCAGNTMILDTKPLM
                     DHSQPVDKFGFGWHAFESYLFGVGARWTSGSAKSYFYTRIDSDVVALDFRSPFHSDTR
                     FQVLLSAYDPGTKKFILAHSEEFYGRKDALMRVDIPVKLRGNTFYRLTILSPTYSPQE
                     R"
BASE COUNT         1054 a         1156 c         1297 g         1005 t
ORIGIN      
        1 aatccgattc ccggcgatga aattgtaggg tacgtcaccc gcgggcgcgg cgtcgcggtg
       61 cactccaact cttgccctaa cgtgcagaat ttgttgtatc aggtagagcg ccgcatcgac
      121 gtggagtggg gcgggacaac cggctccacg tatcccgtca ctttgttgat ccgcgcgcag
      181 gaccggcccg gaatgcttgc cgacattacc gccgtgatca gcgagacggg ctcgaacatt
      241 cgcaccctcg gcagccaccc cgacaatttg cacgcccgca ttgacctcac tctggaaatc
      301 agcgaccgca aacaactcga acggatcatt gcgaatatcc ggaagatttc cggcgtgttc
      361 ggagtagaac gtttgtacaa cgtctgaggt tttagtagga caggcttcag cctgtctggt
      421 ttagatgtct gtaggttgcg gaagtcaaaa gaagacaggc tgaagcctgt cctactcgcc
      481 agttaatctt cggagcacag gcaaaatccg ctgctgccta cgtaggctac aattatccaa
      541 tgcaagcttt tcagccattc acgaatcacg cctggcgatt gccatgtgcc attttgctag
      601 ccgctattgc atttctttcg tcctgcaaat ccgctccgaa ggcggacact gaggaaacga
      661 atcagatggc catgtggctg gacagtgtgc cgcaattaaa aacgctgggc atatcgaacg
      721 cggaagtgaa tgagctgagg aaagcgcatg aggctggact caccgatccg tcgagcgtcg
      781 tgttgataaa gatggcgcgc gaccggcata cgccatttac ggatgggcaa tcagtggccg
      841 acttgttgaa cgcaggatcg actgaagcaa cagttttgca gcttgcccgc ttcaattctc
      901 tgggtatctg ggcgggagag gccagcgcga tgcgattggc cgggctttcc gacaagatta
      961 ttctggctgt ggcggagcgc aggtcgcaag gcttgcctgt gctccccggc gaaaaactgg
     1021 gagaactgaa aaacacaggc gccagcgacg cgacgattct ggactttgtg cagaaaggct
     1081 tgtccgaaaa gtccgtgaac gattatgtcg cgcagcgcaa tcgcgtagcc gggcatagtt
     1141 ttgtttacca gaggcgatct cgaaagggct gacgatcgct gcacactggc gatttcattt
     1201 tgcagcggga ctcttcacgt atatcctcac aagttgaaat taaattcaga agcgcttgca
     1261 tggcgccggc ttgcatgcgg aaattgcgaa tcccatgaaa attgaaatcg tacaggcggg
     1321 gaatccagtg ttgcggcagc gcgcgcgaca attgagcgtg gaggaaattc gcagccgcga
     1381 aacgcaaaaa ctgatggagg cgatgcgcac ctgcatgcac gaggcgcccg gcgttggact
     1441 cgcggctcct caaatcggct tgccaattca gcttgcggtg attgaagacc gtgaggaata
     1501 tcacaaggat gtttccgaag ccctgctcgc ggagcgcgaa cgccgtccgg ttccgtttca
     1561 cgcgatcatc aatcccacga tccaactcat tggcgaggaa aaggcagagt ttttcgaggg
     1621 atgcctcagt gtagccggat attccgcact ggtctcgagg tcgcgcgtcg tgcgcgtaac
     1681 ctgccttgat gagtggggcg tcgcgcaaac gatagaggcc agcggatggt atgcgcggat
     1741 attgcagcat gaaatcgacc acctgaacgg cacgctttat atcgaccgca tgcgcgcgag
     1801 gtcgtatacc acgacggaaa atctggtgga gttctggaag ggcaagtccg tgagcgaaat
     1861 cagggcggaa ttggaaatcg ggcatccggc tgatgcaacg acggaatagt taattcaact
     1921 tgtttcaaga aactcgccgc aggctcgaat gatcaggagg accgtgtgat cgaaactgtt
     1981 gcaacagatc gaggcccgaa ggctattgga ccgtattcgc aggccgttcg cgccaacgga
     2041 ttcgtatttc tctcgggaca aattccgctg gacccgaaaa cgcagcaaat tgtggaaggg
     2101 gacgtggccg tgcagacgga acgcgtcatg gagaatttaa aaggcgtggt ggaagcggcc
     2161 ggatcgtcgc tgcatcgcgt ggtaaaaact acggtatttc tcgctgacat gaatgatttt
     2221 gcggccatga acgaagtgta cgcgcgctat ttcacggcgc atgctccggc gcgatccacg
     2281 gtggaggtgg cgcggcttcc taaaaatgtg cgcgtcgaaa ttgacctgat cgccttgctc
     2341 tagcgcgcgc tatgcgggaa tcgctcagtg agcggcggcc tcctgggcca acgggctcac
     2401 tttggcgcgg cattgttcag cgagagcgtc gacggcgccg cgtccttcgg gctctaaatt
     2461 tggcagcaca gcgtcaagca tggcaatcgc ttcgtgccaa cgactcatgc ccgctagcat
     2521 ggcgccgtag ctaactgcag cctcaggcag atctggattc agttcgagag cccgacgaaa
     2581 tccgcgctcc gcttcgggaa aatgggtcag agcaaattgc gcggtggccc agtcgctcca
     2641 ccgttcggcg gtttcctctt cgcggatcac ttcatcgaga tgagtgaccg cgtctgcaaa
     2701 aaatccttgc tcaaacaagt acaccgcata ttcgtgttgt gagttgatcg acataaaaag
     2761 tcctcctgct tcgtggtctc gcgagggatg aaagcgccgc atttgccggc gttgacgtaa
     2821 gacgtgttcc cgcgtaatga agtgcatttt aaatgccaac tccgcaatgc gaattctgcc
     2881 gcaaagtagc gcgtttcatt gtggaattcg gcctctctgc gacgcgactt tcagggtctt
     2941 taaagccagt ttttgttttg tcgatacaag gtagtgagag aatgtggcga tttcatggtg
     3001 gaataatttc gattatgccc tgcccgtctc caatattccg tgcattcccg aggtgcatat
     3061 gacgcaagcc agactgctga ttctggaagt cacttcccac tgcaacatgc actgcacatt
     3121 ttgcccatcg gacgatcttg ttcgacgcaa gggaaatgta agcgatgagc aggctcggca
     3181 gttgattcgc atggcctccg aagtgtctcc aggggtaccg atcatgttca atgtgctagg
     3241 cgagccgttt ctgaacaaga aattgtttga gtacataggg ttgtgcgaag aaaataaagt
     3301 tcccgtcgtg ctcattacca acattacgct gctcaacgag gatcgcctgc ggaagctttt
     3361 tacgtacagc aacgtgcatt tggccatgag tttgcacacg ccgaccgaaa gagccttcgc
     3421 cagtcgcggc tacaaaaaga tcgacaactt ccgggactat ctcgagatgg tgtgtaatac
     3481 agtcgaagcg aagttccgcg cggggagcaa gaccacgatt gaaatttatc tcgcgagcga
     3541 acttatcgaa aaccttatgc agaacgacgc gggttcccgg ctgtggacca tgtatgacga
     3601 ccctgcggaa tacaaagaag gctgggattt gtgcgcgaaa cggttgaccg aacttagcgc
     3661 caaaatcgcg cgcgactacc cagaagtttt tacggaaggc atggaaaaat ttcggcgcga
     3721 tcaaaaagat ctagtgaaag ccggcgaaat tgttttccgg gcggaagatc tgccggaatg
     3781 gcggctcgaa ggagaaatgt ccggctggct gtgcgcgccc ggcgtctttg tgcgccgcaa
     3841 aggcttcggc atgtgggcgt accatgaaaa attcgtacag cggcacgcgg aggcggcccg
     3901 gttcgttttt cacgaggagc gtacggaacc gttccagtgc gtgggtgcgc taacctttgg
     3961 aattctttca gatggcacct acacgctttg ttgccaggat gttgaaggtc aaatggacat
     4021 aggtaacatc gccgacatgg agccgcgcac cgcattcgaa tcaccacgcc gccaggaaat
     4081 tatagacaat tgtgcgactt cgagggtttg ccgtcgctgt gcaggaaaca cgatgatctt
     4141 ggacacgaaa ccactaatgg accactccca acccgtcgat aaattcggat tcggctggca
     4201 cgcgttcgaa tcttaccttt ttggggttgg cgcgcgttgg acgtcgggca gtgcgaaatc
     4261 gtatttttac acgcgaattg attcggatgt ggtcgctctg gatttccgct ctcctttcca
     4321 ttccgacacc agatttcaag tgctgttgag cgcttacgat cccggtacaa agaaatttat
     4381 actggcgcat tcggaagagt tctacggaag gaaggatgcc ttgatgcgcg tggatattcc
     4441 tgtaaagctg cgggggaata cgttttaccg actgacgatt ctttcgccaa cgtacagtcc
     4501 ccaggaacga ca
//
LOCUS       DASZMB010000241         9311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_800142, whole genome shotgun sequence.
ACCESSION   DASZMB010000241 DASZMB010000000
VERSION     DASZMB010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 9311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9311
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800142"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2003)
                     /locus_tag="VG272_11325"
     CDS             complement(<1..2003)
                     /locus_tag="VG272_11325"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWF14319.1"
                     /translation="MANISFILNGKRTTCSYEPGMNFLEVLREECGIVSAKNGCAPEG
                     TCGCCTVLVDGRPAMACLRKPEQMVGHDVVTLEGIPEDMRRVLSESFVVEGGVQCGFC
                     IPGIVVRATSLMQQGTACNRDAVAKALDAHVCRCTGYSRIVDAVLTAGEAWNDGKRLT
                     SNVPRRHHFFGEEFGMSRNPEFALSGSGKTTNGGGIGSSPLRYGGLDQTLGEKAFVDD
                     MRAPEMLHGAMVLSEHPRAKVLAIHTENAAAKAGVVRIFTAADAFGQRGAGIAVPDLP
                     IFVAVGETTCCVGDFLAMVVADTDFHAQQAAKQVIVDYEVLTPLTDPFEALKPGAIQV
                     HGPDTLRPAPNLVGMTAFSRGDVDAALASATHVIDHTWQTQAIDPAFLEPEACLAIPQ
                     GRGVKIHTQSQGSIHDQQQLSAILNLPPDDVEIALAASGGAFGAKEELSIQAQTALAA
                     LLLQRPVKTVLTRKQSTQHHVKRHPMTIHLTVGADAEGNLLAVRTRTVGDAGGYAGTS
                     AKCLLREACHSCGPYRVPNVDVESKAVYTNNPTSGAMRGFGSNQAHFAMEGAMDLLAE
                     KVGVDGWEIRMRNILNPGDAFATGQIMRNSVLGLRRSLEAVKEVYKNAKYAGIACGIK
                     STGIGNGTIDSGFIKIRVLGGPRIEILNGYTEMGQGIYTATRQA"
     gene            2366..3562
                     /locus_tag="VG272_11330"
     CDS             2366..3562
                     /locus_tag="VG272_11330"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWF14320.1"
                     /translation="MKITKWAVLLSVTSVAFFGISLAARAQSRATGTPPPAIHTFSTA
                     DLASTGFFYVGGQYVGAPGKEVMDGAEYVEVMVPKKIRHSYPIVFFHGAGQTGTDWLQ
                     TPDGRPGWAYYFTKLGYTVYMVDFPARGRSTYNPSADGDGTLTIRTALQLEQIWTDIG
                     AQGNWPQAKKFSQWPGDGPNKGKMGDPIFDDFARTQVQFLQGGKQAKLNLDAHVALLD
                     KIGSPVILITHSQGGEFGWQVADARPKLVKAIITAEPAGPPIKNVDTAKIAYSGPGSL
                     TWGVTSLPLHYDPPVSDPAELQPVLEAKSEGPDIVPCYLQKEPAHKLVSLKDIPVLDV
                     STQASYHRVFDSCIAKWLMQAGVKTEYIKLEDVGITGNEHELMLDKNSDQIAKYFADW
                     LEKNVH"
     gene            complement(3652..4470)
                     /locus_tag="VG272_11335"
     CDS             complement(3652..4470)
                     /locus_tag="VG272_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14321.1"
                     /translation="MRNHWVPCILFFAAALFSSSGMRAQTAPKKAAMAKSEAASKPVP
                     KMADGTPDFSGVWEGHMPASTRKWAIYSFTGDIPEMTPFGKAIYEKTKPSWGPRAVED
                     STDMVNPTTGNEIGCFPTGVPRIYVQPFPMEILQVPGRVVQLFEFNHFVRQIYTNGPH
                     EHAKDLDPTWMGDSVGWWEGDTLVIDSVAFNDKTWLDRAGLPHSDQLHVIEHISRPNF
                     KTLVVHITIDDPKAYTKTWEGERIYELEPDWKLTEMICEDNITFNDLKKAGKEQ"
     gene            complement(4553..4951)
                     /locus_tag="VG272_11340"
     CDS             complement(4553..4951)
                     /locus_tag="VG272_11340"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HWF14322.1"
                     /translation="MRKVLLASGFLAVCLFAISEPALAHHGGAAYDSSHPMTLKGTVK
                     EFQFIQPHPLITLEVKDDQGATAEWSVEMTAPNHLVHFGWNGKKLKPGDQITVVGLPS
                     KSGLKVLNLRKISWSTGEEIPLGPPPEPGQ"
     gene            5332..6597
                     /locus_tag="VG272_11345"
     CDS             5332..6597
                     /locus_tag="VG272_11345"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWF14323.1"
                     /translation="MDSLLNVSRRSLVKMGLAAAGLPVLTKAFGASALAQDASSEAGK
                     KGAIGGRDERELYTGKGLQGGGQLEIRLTQSLYTSDPDELEVSQRMKPFDLESWIVEW
                     TRVAEKNEQEAEKLASDGYKVTANEYYLRASRFYREACWPQPVDEPRMMATYKKMRET
                     FDKAWKMTKPPFERVQINYEGKTLEGYFRKPNGPQGKKYPVVIPFQGADTMAEATIMG
                     GGTYTSRGMAYLVVDFPGQGGAMRLHDLHLPPDTDRVAKALIDYLETRPDVDATRVGM
                     QGISMGGYGVPRAASGEKRIKAALMSSGSYDLKSDLFEYYPPIQERVRWIIGARDLAD
                     ARKKLDDYNLGGGRAEKIEAPMLIGYSKDDRIMDPAGALRLYEAAKNSKRDMVEGTGH
                     VQASNAGGPRSMRPVVFPDWAAKTLGAEV"
     gene            complement(6724..8388)
                     /locus_tag="VG272_11350"
     CDS             complement(6724..8388)
                     /locus_tag="VG272_11350"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWF14324.1"
                     /translation="MSKMSRREFTKGAAALAPLAFAPFSLTAQTAPSDKFDIVVAGAG
                     HNSLVTAAYLAKAGYRVVVLEYRPQVGGGVKTEEVTLPGFKHDTCSTAHGGIQANPVM
                     RNDELHLIRDYGIEYIQADPIYHMPFPDGSYITQWRDLDWTCEEFAKFSKKDAAAYRR
                     MHDENEAIRPVFDGNGLTPPGFGGKTVAEKLAEHPQGKIWQRRLAMSQWEILRDNFED
                     DHCRAFMMGTPPSMSPPQYPMSGRSAYSAIRTSRPIPKGGSGILTQALAKFIEAHNGV
                     VLTSKAVTRLIIENGKCAGVECADGSSYRGEKAVLSTIHVKQLVDMAPRELWGHDFLD
                     GVDTWEGEISEIVAHYASKEPPKYPVKGGTLAVCESAILVSPARLLRYAHDFPNATVG
                     LDDPPLTIFSSTVADPSRAPAGMHTIKVIGLQPYDLKEGAKHWDSIKDEVAEANLNYL
                     RKFAPNLTDDKILGKFIVSPLDIERRNPHMWHGSVHAGSSEPAQSGNMRPMPGWAQYR
                     MPIPGLYQTGACTYPGGSVTGAPGRNAAMVMLKDFGTSIEEVVKKG"
     gene            complement(8542..>9311)
                     /locus_tag="VG272_11355"
     CDS             complement(8542..>9311)
                     /locus_tag="VG272_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14325.1"
                     /translation="GSSYRAEKAVLSTIHIKQLVDMAPREMWGQDFIDGVDTWQAEAQ
                     MFAVNYATTEPPTYAVDGGVIKPANSAIVAGSESILRVAYDFSRGAVNWEDPPLAVHC
                     TTSADPTRAPAGMHTIKVVGWQPYELPEGPEHWDVIRKVVADTCLAHLRRFSTNLTQD
                     KILAEVIQSPLDLERMNPHNWHGSCHAGAQSPAQAGALRPMAGWSQYRMPIPGLYQTG
                     ACTYPGGSVTGAPGRNAAVVMLKDFGTSIEQVVSKKS"
BASE COUNT         2100 a         2549 c         2561 g         2101 t
ORIGIN      
        1 gcttgccgcg tagccgtata aattccttgt cccatctcgg tatacccgtt cagaatttcg
       61 atgcgagggc ctccgagcac gcgaatcttg atgaatccgc tatctattgt accgttgcca
      121 atacccgtgc tcttgattcc gcaagcaatc cccgcatatt ttgcgttttt atagacttcc
      181 ttcactgctt ccagcgagcg gcgcaggccc agcacgctgt tcctcataat ctggccggtc
      241 gcaaacgcgt cgccaggatt caaaatattg cgcatgcgaa tttcccagcc gtcgacgccc
      301 actttttcag cgagcagatc catggcgcct tccatcgcaa agtgagcctg attgctgccg
      361 aatccgcgca tggcgccgct ggttggattg ttggtgtaga ccgctttcga ttcgacatcc
      421 acgttcggca cgcggtaagg accgcacgag tggcaggcct cgcgcagcaa acattttgcg
      481 ctcgtgcccg catatcctcc cgcatcaccc acggtccgcg tgcgcaccgc cagcaaattt
      541 ccttcggcgt cagcgccaac cgtaagatga atcgtcatcg gatggcgctt cacgtgatgt
      601 tgcgtagatt gcttgcgtgt aagaacagtt ttcaccggac gctgcaaaag cagcgcagcg
      661 agcgcagtct gcgcttgaat tgataattct tctttcgcgc caaaagctcc gccgctcgcc
      721 gccagggcga tttcgacatc gtcaggtggc aaattcaaaa tagcggaaag ttgctgctga
      781 tcgtggatcg atccttggct ctgcgtatgg atcttgacgc ctcttccttg cggaattgcc
      841 aggcacgcct caggttcgag aaacgcaggg tcgatagcct gtgtttgcca cgtgtgatca
      901 atgacgtggg ttgcggaagc caacgcagca tctacatcgc cgcgcgagaa cgcggtcatt
      961 cccaccagat tcggcgcggg acgcagagtg tcaggcccat gaacttgaat cgcgccgggc
     1021 ttcagcgcct caaacggatc ggtcagcggc gtcagaactt cgtaatcgac tataacttgc
     1081 ttagcggctt gctgcgcgtg gaaatccgtg tcggccacaa ccatggccag aaaatcgccc
     1141 acgcaacagg tcgtctcgcc cacggccacg aaaatcggca ggtcgggaac cgcaattccc
     1201 gcaccgcgct gtccaaatgc gtcggctgcg gtaaaaattc gaaccacgcc cgccttcgcg
     1261 gccgcatttt ccgtatgaat cgccagaact tttgcgcgag gatgctcgct caataccatt
     1321 gcgccgtgca gcatttctgg tgcccgcata tcatccacaa aagccttctc gcccagagtc
     1381 tgatcgagtc cgccatagcg cagtggcgag ctgccaatcc ccccgccgtt tgtcgtcttg
     1441 ccgctgccag ataatgcgaa ttccggattg cggctcatcc caaattcttc gccaaaaaaa
     1501 tgatgccgcc tgggcacatt gctcgtcagg cgcttcccat cgttccaagc ttcgcccgcc
     1561 gttagcaccg catcgacgat tcgcgaatat cccgtgcagc ggcaaacgtg cgcgtccagc
     1621 gccttggcca cagcgtcccg attgcatgca gtgccttgct gcatcagcga agtggctctg
     1681 actacgatgc ccgggatgca gaagccgcat tgaacgccac cttcgacaac aaacgattca
     1741 ctcagcaccc ggcgcatatc ttcgggaatt ccttcaagcg tcaccacatc gtggcctacc
     1801 atctgttcag gcttgcgcag acaagccatg gccggacgcc catcgacgag gacagtgcag
     1861 cagccgcatg tgccttcagg cgcgcaccca ttcttggctg aaacgatgcc gcactcctcc
     1921 ctaagcactt ccagaaaatt catgcccggt tcataggagc aggtagtccg tttgccgttt
     1981 aatataaagc taatgtttgc cataagttag tcgttatatg tttgaagcca tatatgccgg
     2041 atatttagtg taccgcgcct tcatccgtgg agctaacttt ggcagaaacg attgtgatta
     2101 aagaactgac atagccccac aatggttaga tgtttcatag gagaataccg atacgttgaa
     2161 tctgaagttt gtgtgtcttt agtatcccga cggcaagatg gccgaccatt cgccatctcc
     2221 ttgatttcag ggataataac gttaagattt gcaggtgact tgcgatgaaa attcgaagga
     2281 tgcaatccag cgctgaggta tactttcacg gaacgaaacg ccaatcaaaa attcctgtca
     2341 tgcaccacaa acaaggggac cagtcatgaa gatcacgaag tgggcagtgt tattatccgt
     2401 aacctctgtt gcgttctttg gtatttcgtt ggccgcgcgg gcacagagtc gcgcgaccgg
     2461 tacaccaccc ccagcgattc atacattctc aacggcagac ctcgccagca ctggcttctt
     2521 ttatgtgggt ggccaatacg tgggtgcgcc cggcaaagaa gtcatggatg gtgcggaata
     2581 tgtggaagtg atggtgccga agaagattcg ccattcttat cccatcgtgt tttttcatgg
     2641 cgcaggacag actggaactg attggttgca gacccctgat gggcgtccgg gttgggccta
     2701 ttatttcacc aagctgggat acacagttta tatggtcgat tttccggcgc gcggccggtc
     2761 cacctacaat cccagcgcgg atggcgatgg cacactgacg attcggactg cgctgcaact
     2821 ggaacaaatc tggacagaca ttggcgcgca agggaactgg cctcaggcta agaaattttc
     2881 gcaatggcct ggggatggcc cgaataaggg caaaatgggc gatccaattt ttgacgactt
     2941 cgcgcgcacg caagtgcaat ttctgcaggg cggaaaacaa gccaaactga atctggatgc
     3001 gcatgtggcg ctgctcgaca agattggctc tcccgttatt ttgatcacgc actcacaggg
     3061 cggggaattc ggttggcaag tggccgacgc gcgccccaaa ctcgtgaagg caatcattac
     3121 cgcggagcca gccggtccgc caattaaaaa tgtggacacg gcaaagattg catacagcgg
     3181 gccgggcagt cttacgtggg gagtcacatc attaccgctt cattacgatc cacccgtcag
     3241 cgatccggca gaattgcagc ctgtgctcga agctaaatcg gaaggtccgg atattgtgcc
     3301 ctgctacctg cagaaagagc ccgcgcacaa acttgtgagc ctgaaagata ttccggtgct
     3361 cgacgtttcc acgcaagcga gctatcaccg cgttttcgat tcgtgcattg caaaatggct
     3421 catgcaggcc ggcgtgaaaa cggaatatat aaagttggaa gacgtgggaa ttacaggcaa
     3481 cgaacatgaa ctgatgctgg ataaaaatag cgatcagatt gccaagtatt ttgcggactg
     3541 gctggagaag aacgtgcact gatttggaat taagtggtgc gggcttcagc ctaagggttt
     3601 tagtccttgc aaggcctaaa atccacaggc tgaagcctgt gctactaaaa cctactgctc
     3661 tttgcctgct tttttcagat cattgaacgt gatgttatct tcgcaaatca tttccgtgag
     3721 cttccagtca ggctcgagtt cgtaaattcg ctcgccttcc catgtctttg tatatgcctt
     3781 cggatcgtca atcgtgatat gcacgacaag cgttttgaag ttgggacgcg aaatgtgctc
     3841 aatcacgtgc aactgatcgg aatgcggcaa tcccgctcga tcgagccatg ttttgtcgtt
     3901 gaacgcgacc gaatcgatca ccagcgtatc gccttcccac cagccaacag aatcgcccat
     3961 ccaagtggga tcgagatcct tggcatgctc gtgcgggcca ttggtgtaga tctggcggac
     4021 aaagtgattg aattcaaata gttgaaccac tcgcccgggg acttgaagaa tttccatggg
     4081 aaaaggctgc acgtaaattc gcggaactcc ggtagggaaa cagccgatct cattgcccgt
     4141 ggtcggattc accatgtcag tggagtcttc caccgcgcgc gggccccagg acggtttcgt
     4201 cttttcgtat attgcttttc cgaatggcgt catttccgga atatcgcccg taaacgaata
     4261 aattgcccac ttacgcgtgc tggcgggcat gtgtccttcc cacacgccag agaaatcggg
     4321 agtgccgtca gccattttcg gaacgggttt ggatgccgct tcagattttg ccatggcggc
     4381 ttttttcggc gcagtctgtg cccgcattcc gctggacgaa aacagcgcgg ctgcaaaaaa
     4441 tagaatgcaa ggcacccaat gattccgcat gtttggaatt cctcctgtcg ttccaatcaa
     4501 gttcaagctc agcgcacgat gaagcattta aatctggcgt ggaacgatgt ttctattgtc
     4561 cgggttccgg tggaggtcct agaggaattt cttcgccagt ggaccaggaa atttttctca
     4621 gattcagaac tttcaaaccg cttttggagg gaagtcccac aacggtaatc tggtcgccgg
     4681 gtttaagctt cttgccattc catccaaaat gcaccaaatg attcggcgcc gtcatttcca
     4741 cggaccactc ggctgtcgcg ccttgatcgt cctttacttc gagcgtgatg agcgggtgcg
     4801 gctgaatgaa ttgaaattcc ttcactgttc cctttaaagt catggggtgg gacgagtcgt
     4861 aggccgcgcc gccatgatgc gcgagcgccg gttcggaaat agcgaagaga caaacggcca
     4921 gaaaaccaga agcgagcaga actttcctca ttggtgaact cctcccatta tcgcggagtg
     4981 tgtcattatt ttatctggcg tgtcaatcaa aatactgtct gttcttaagc gtatgattaa
     5041 tttcagccgg atccaaaatt atcagaaatg tatattccgc ataagctatg tgattaagaa
     5101 aatgcgcatg gcctacggtg aaaccttgct tgacaggctt catgctctgc cgtatggtct
     5161 tagaaaaatc gccataatcg acgggtgtga aatgttgttt tcggcgaagt ggcgatagaa
     5221 aaaatctgaa acgcgcgcac ctcgaacgac agtcttattc tcaacagcca gcaggcttca
     5281 cacggtccaa aaccggtacc gaaattcgcg taacgaatgg agggttgagc catggattca
     5341 ctattgaatg tcagccgcag aagcttggtc aagatgggac tggcagcagc cggacttccc
     5401 gtgcttacca aagcgttcgg ggcgagcgcg ctggcgcagg atgccagcag cgaagccgga
     5461 aagaaaggcg cgattggcgg ccgcgacgaa agagagcttt acaccggcaa gggactgcag
     5521 ggaggcgggc agctggagat tcgcctgacc caatcccttt acacaagcga tcccgacgag
     5581 ttggaagttt cgcaacgaat gaaaccgttc gatctggagt cgtggattgt ggagtggacg
     5641 cgagttgcag aaaaaaatga gcaggaggcc gagaaactcg cttccgacgg ttacaaggtt
     5701 acggccaatg aatattacct ccgcgcttcc aggttctacc gcgaagcctg ctggcctcaa
     5761 ccggtcgatg agcctcgcat gatggccacc tacaagaaaa tgcgcgagac attcgacaag
     5821 gcctggaaga tgaccaagcc gccgttcgaa cgggtccaga tcaactacga aggaaaaacg
     5881 ctggaaggat atttccgcaa gccaaacggc ccccagggaa agaaatatcc cgtcgtgatt
     5941 ccattccagg gcgcggacac catggctgag gcaacgatca tgggcggcgg cacctacacg
     6001 agccgcggca tggcttatct ggtcgtcgat tttccgggac agggtggcgc catgcgattg
     6061 catgatctgc atctgcctcc ggacacagac agagtcgcga aggcgttgat cgattatctc
     6121 gagacgcgac ctgatgtgga tgccacgcga gtgggcatgc agggcatcag catgggcgga
     6181 tacggcgtgc cacgcgccgc gagcggcgag aagagaatca aggctgcatt gatgagcagc
     6241 ggctcttacg acctgaaatc ggatttgttc gaatattatc cgccgattca ggaacgcgtc
     6301 cgctggatca tcggcgcaag ggatttggcc gatgcgcgca aaaagctgga tgattacaac
     6361 ctcggcgggg gcagagcgga gaagatcgaa gctcccatgc tgatcggcta tagcaaggat
     6421 gaccgcatca tggacccggc gggcgcttta cggctgtatg aagctgccaa aaattcgaaa
     6481 cgtgacatgg tggaaggcac aggccatgtg caggcttcga acgctggcgg gccgcgctcc
     6541 atgcgaccgg ttgtgttccc tgattgggct gcgaaaacgc tcggggcgga agtttaggtt
     6601 tacggggcat aggcttcagc ctgtgcgctt ttgatctttg ccaaaacaaa agaaatctaa
     6661 accgaacagg ctgaagccta ttccacgcaa accgggacag cctgtttaat tttacagtcg
     6721 tgcctaaccc ttcttcacga cttcctcaat gctcgtcccg aaatctttaa gcatcaccat
     6781 ggccgcgttg cgtcctggcg ctcctgtgac ggagccgccg ggatacgtgc acgcgccagt
     6841 ttggtagagg ccgggaatgg gcatgcgata ttgcgcccag ccgggcatgg gccgcatatt
     6901 gcccgattgt gctggttccg acgatccggc atgcacgctg ccgtgccaca tgtgcggatt
     6961 gcggcgttcg atatcgagcg ggctgacaat aaatttacca aggattttat cgtccgtcag
     7021 attaggtgcg aattttcgca ggtaatttaa attcgcttcc gcgacttcgt cctttatcga
     7081 gtcccaatgt ttcgcgcctt ctttcagatc gtagggctgc agtccgatca ctttgatcgt
     7141 gtgcatgccc gcgggagcac gcgacggatc ggcgaccgtg cttgagaaaa ttgtgagcgg
     7201 cggatcgtcg aggcccaccg tcgcgttggg gaaatcatgc gcgtaacgca acaatcgcgc
     7261 gggagacacg agaatggccg attcgcaaac cgcaagcgtg ccgcccttta cgggatactt
     7321 gggtggttct ttggaagcat aatgcgcgac gatttcggaa atttcgcctt cccatgtgtc
     7381 cacgccatcg aggaagtcat ggccccacaa ttcgcgcggc gccatgtcta caagctgctt
     7441 gacgtgaatc gtggatagca cggctttctc gccgcgatag gaactcccgt ccgcgcactc
     7501 gacgcccgcg catttcccat tttctatgat cagccgcgtg acagccttgc tggtgaggac
     7561 tacgccgtta tgcgcctcga tgaattttgc gagcgcctgc gtgaggattc ccgaccctcc
     7621 tttgggaatg gggcgactgg tgcgaatcgc cgaatacgcg ctgcggccgg acatcggata
     7681 ttgcggcggc gacatactcg gaggcgtgcc catcatgaag gcgcggcaat gatcgtcctc
     7741 aaaattatcg cggagaattt cccactgaga catcgcgagg cgacgctgcc agatttttcc
     7801 ctgcggatgt tcggccagct tttcagcgac tgtttttccg ccaaatccgg gcggggttaa
     7861 gccattgcca tcgaacacag gcctgatcgc ttcgttttcg tcatgcatgc gacgataggc
     7921 cgcggcatct ttcttcgaaa acttggcgaa ttcttcacag gtccaatcca gatcgcgcca
     7981 ttgcgtgatg taactgccat ctgggaaagg catgtgatag atcggatcgg cctggatgta
     8041 ctcgatgccg taatcgcgaa tcaggtggag ttcgtcattg cgcattacgg gattggcctg
     8101 tatgccgccg tgcgcggtcg agcaagtgtc gtgcttgaat cctggcagcg tgacttcttc
     8161 ggtcttcacg ccgccgccga cctgcgggcg atactccagc actacgacgc ggtatccagc
     8221 ctttgcaaga tatgcagcgg tcacgaggct gttgtgtccc gcgccggcga cgacgatatc
     8281 gaatttgtcg gacggagcgg tctgcgcggt cagactaaat ggcgcgaatg ccaacggcgc
     8341 gagtgccgct gctcctttgg tgaattcccg ccgagacatt ttgctcatgt gatcctcccg
     8401 atgcgcaggt tcgtgtagcg cccgccttca ggcgggcgat cgttgatcgc ccaaatcaaa
     8461 aggtcaaaac atagaaacaa aacaaaaatt caaaacacct gcccgcctga aggcgggcgc
     8521 cacacgatca aaaattctcg atcacgattt cttacttact acctgctcga tactcgtgcc
     8581 gaaatccttg agcattacga ccgcggcatt gcgtcccggc gcaccggtga cggaaccgcc
     8641 gggatacgta caggcgccag tctggtaaag gcccgggatt ggcatgcggt attgcgacca
     8701 gccagccatt ggccgcaggg cgcccgcttg cgccggactt tgtgcgccag cgtggcaact
     8761 gccatgccag ttgtgcggat tcatgcgctc cagatcgagc gggctctgaa ttacttcggc
     8821 gagaatcttg tcctgcgtaa gattcgtcga aaagcggcgc aggtgggcta ggcaagtgtc
     8881 agccaccact tttcttatga cgtcccagtg ttccgggcct tccggcaatt cgtagggttg
     8941 ccagccaacg actttgatcg tatgcatgcc ggcaggcgcg cgtgtggggt cggcggaagt
     9001 cgtacaatgg accgctagcg gcgggtcttc ccaattcacg gctccgcgcg agaaatcgta
     9061 ggcaacgcgc aaaatacttt ccgatccggc aacaattgcg gaattcgctg gcttgattac
     9121 gccgccatca actgcataag ttggaggctc agtggtcgca tagttgactg caaacatctg
     9181 cgcctctgcc tgccacgtgt caacgccatc gatgaaatcc tggccccaca tttcgcgcgg
     9241 cgccatgtcg accaattgct tgatgtggat agtcgagaga actgccttct cggcgcgata
     9301 ggacgagccg t
//
LOCUS       DASZMB010000242         3595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1056087, whole genome shotgun sequence.
ACCESSION   DASZMB010000242 DASZMB010000000
VERSION     DASZMB010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1056087"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="VG272_11360"
     CDS             <1..591
                     /locus_tag="VG272_11360"
                     /inference="COORDINATES: protein motif:HMM:NF013874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14326.1"
                     /translation="SLHPVGARHAVPLPGEATPIAQAGTAIVLLSASGKLFRQETARR
                     LAQLDRIILLCGRYEGVDERVAQHLATDELSVGDYVLSGGELGAALILDAVTRLLPGA
                     LGNEDSTINESFSAVEAAGADEEFSMSYQSPLTIHQPPVTSHLLLDYPHYTRPAEFRG
                     WPVPEVLLGGNHEEIRKWRQKKALEKTKRNRPDLLP"
     gene            1096..2079
                     /locus_tag="VG272_11365"
     CDS             1096..2079
                     /locus_tag="VG272_11365"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HWF14327.1"
                     /translation="MADPGASTRKLTMADIHAAAERLKNWGKWGPDDQIGTLNYVQPE
                     DIVAATRLVQKGKVISLALNFDNNGPQGGKSKYPAMGRFNPIHLMLRTGTDAYSGVLD
                     SRKIRGSDDVIMMPTQAGTQWDGLGHIFYGNHMWNGYDCRTVTSAGAQKCGIEQTKAR
                     MVGRGVLFDIPRMLGMEYLPDTFAITNEMLDQAEKHFGVEVRRGDYLLVRTGQMEAML
                     SAKSWDGYSGGDAPGLAFETLDWLHKKQVAGIATDTWGAEVRPNESDDANQPWHWISI
                     PIMGLTVGEIFDLAELARECADDKKYEFLFVAPALPVTGAVASPVNPLAIK"
     gene            2249..>3595
                     /locus_tag="VG272_11370"
     CDS             2249..>3595
                     /locus_tag="VG272_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWF14328.1"
                     /translation="MTTTSVGDSGAMTLAQAMAKILRAYGAEYIFTLTGAPQEALIEM
                     QNREGVRVVLGRSERSVLAMGDAYARVTGKPTFGMVQFGPGATYLPASIIDAYWASSP
                     LVSLSGTTTTKTRYRFEYQEVEQTSMFSAMTKWAGDLPQPERIADVLRTAIRASISGV
                     PGPVYLGIPIDWYGKKLAFPQEIYAEPAFMRTPALRVSPMASDVERAMASLSSAKKPV
                     LIAGGGVILSEAWAELTALAETLNIPVVTTMAGKGSIADIHPLAVGVCGRYSRKVAND
                     TLADADFCLAIGTKLSSMGTNVFQYPGKNTRIVHFDLDANSLGRTYREELSSVGDARE
                     ALKMLRDAAIAAKVNGSNWPDWTKQVQGNVASWMADLERESRTPMHEGKLNPYHIMRL
                     IDQHLAGDDMLVADTGYMAAWAVTVLQQKKMGRNTLRAAGSLGWAFPAAFGAKLAVG"
BASE COUNT          849 a          990 c         1010 g          746 t
ORIGIN      
        1 tcgcttcacc cggtaggggc acggcatgcc gtgcccctac cgggtgaagc gacgccaatc
       61 gcacaggctg ggacagcgat tgtgcttctc tccgcgtcag gaaaattatt tcgccaagaa
      121 acggcgcgcc gcctcgcaca actcgatcga atcattctgc tttgcggccg ctatgaagga
      181 gtggacgagc gcgtcgcaca acatctcgcc accgacgaac tttccgtcgg cgattacgtg
      241 ctttccggcg gcgaactggg agccgcactc atcctcgatg cggtgacgcg cctgcttcct
      301 ggcgcattag gcaacgaaga ttcaaccatc aacgaatcgt tcagtgcggt cgaagccgcc
      361 ggtgctgacg aggaattttc catgagctac cagtcacccc tcactattca ccaaccaccg
      421 gtcacgagcc accttctcct cgattatccg cattacacgc gccccgccga attccgcggc
      481 tggcccgtgc ccgaagttct gctaggcggc aaccacgaag aaatccgcaa gtggcgccaa
      541 aaaaaagccc tggaaaaaac caaacgaaat cgcccggatt tgttgcccta aaatcttttt
      601 tctatttacc aatattggga ataggtctga ggaacacaag ctgcagaaaa agtatctcat
      661 tctgtcattc cgagcaaagc gaggaatctc tccttctttc ttttcataca ttgaaatcga
      721 aaagagattc ctcgctttgc tcggaatgac agaataaacg actttttccg caacctattt
      781 agccgctgaa gcttcaattt tgcagttctt acacagaccc ttcagcctgt ggaactcagg
      841 ccttgtaaag gcttaacccc acaggctgaa agtgtcaagt ctcaggacat tgcttacaca
      901 tcggtctcag gacatccttt acactttttc aggggcagtt ccgccctgaa gcccgcgcta
      961 cataattcca cttcacttcc atttgcgtga ccgcaaaaac cgcagacacc tcgctttcga
     1021 ttccagtaca atgacgggct gaaattataa gttcaaataa tcctataact acgcatcaac
     1081 atcattggag ggcggatggc agacccggga gcatcaacgc gcaagttgac catggccgat
     1141 atacacgcgg cggccgagcg gctaaaaaat tggggaaaat ggggacctga cgatcaaatc
     1201 ggaactctga actacgtgca gccggaagat attgtcgcag caacgcggct ggtgcaaaaa
     1261 ggcaaggtaa tctcccttgc gctgaacttt gacaacaatg ggccgcaagg cgggaagtcg
     1321 aaatatccag cgatggggcg attcaatccc attcatttga tgttgcgcac gggaacagat
     1381 gcgtactcgg gggtgctgga cagccgcaag attcgcggct cggatgacgt gatcatgatg
     1441 cccacgcaag ccggcacgca gtgggacggg ctcggacata tcttttatgg caatcacatg
     1501 tggaatgggt acgattgccg cacagtgacc tcggcgggcg cgcagaaatg cggcattgag
     1561 cagaccaagg cccgcatggt gggacgcggc gttctgttcg atattccccg catgcttggg
     1621 atggaatatc tgccggacac tttcgccatc accaatgaaa tgctggacca ggcagaaaag
     1681 cattttgggg tggaagtgcg gcgcggcgac tatttgttgg ttcgcacagg ccaaatggaa
     1741 gccatgcttt ccgcaaagtc gtgggatggc tattcgggcg gcgacgctcc cggccttgcg
     1801 ttcgaaacgc tggactggct gcataagaag caagttgcgg gcatcgccac ggacacctgg
     1861 ggcgcagaag tacggcccaa cgaatcagat gatgccaacc agccctggca ctggatttcc
     1921 attcccatca tggggctcac ggtaggcgaa attttcgacc tcgcggaact ggcgcgagaa
     1981 tgtgcggatg acaagaagta cgaattccta tttgtcgcgc cagccctgcc ggtaacgggc
     2041 gccgtagcgt cgccagtaaa tccacttgcg atcaagtgaa acaaaaactt aacacagagg
     2101 acacagagga aagacggaga aaagctgtag gaaatataga gaaagaatga ttccgaggaa
     2161 tgggtgaatg gtggggaaat tttttcctct tttttccctc tgcgttttct gtgttgagat
     2221 ttttttcaat tcaattgagg acaagacaat gaccactacc tcggtgggtg actcaggagc
     2281 aatgacgctg gcgcaagcca tggcaaaaat cttgcgcgcg tatggagcgg aatacatttt
     2341 cacgctgacg ggcgcgccgc aggaagccct gatcgagatg cagaatcgcg agggcgtgcg
     2401 cgttgttctt ggccgcagcg agcgttccgt gctcgccatg ggcgatgcgt atgctcgcgt
     2461 aaccggcaag ccgacatttg ggatggtgca gttcggcccc ggcgcgacct atttgccggc
     2521 gtccatcatc gatgcctact gggcatccag cccgcttgtt tccctcagcg gcacgaccac
     2581 tacaaaaacg cgctaccgtt tcgaatacca ggaagtcgaa cagacttcga tgttctccgc
     2641 gatgaccaag tgggcgggag acctgccgca gcctgaacgc atcgccgacg tgttgcgcac
     2701 ggcaatccgc gcttccatca gcggagttcc cgggcccgtg tacctcggca ttcctatcga
     2761 ttggtatggc aagaagctgg ccttcccgca ggaaatctat gcggagcctg catttatgcg
     2821 tactcccgcg ttgagagttt cgcccatggc gtcggatgtg gagcgcgcca tggcgtcgct
     2881 ttccagcgcc aaaaaacctg tgctgattgc aggtggaggc gtcatccttt ccgaagcctg
     2941 ggcggaactg accgcgctgg cagaaactct gaacattccc gtggtaacca ccatggccgg
     3001 caagggcagc atcgctgaca tacacccgct ggccgtcggt gtatgcgggc gctattctcg
     3061 caaggttgcg aatgacacgc tcgcggacgc ggatttctgc ctggcgatcg gcacaaaact
     3121 aagctcgatg ggaacgaacg tgttccagta ccccggcaag aacacgcgaa tcgtgcactt
     3181 cgatctcgat gcaaattcgc tcggacgcac gtatcgcgag gagctgtcat cggtcggcga
     3241 tgcgcgcgaa gcattgaaaa tgttgcgcga cgcggccatt gcggcgaagg tcaacggcag
     3301 caactggccc gattggacaa agcaagtgca ggggaatgtt gcatcatgga tggcggatct
     3361 cgaacgagaa tcccgcacac cgatgcatga aggaaagctg aatccgtacc acatcatgcg
     3421 tctgatcgat cagcacttgg ccggcgacga tatgcttgtc gcggacaccg gatatatggc
     3481 ggcatgggcc gtgactgttc tgcagcaaaa gaagatgggt cgcaacacac tgcgcgcggc
     3541 ggggtccctt ggctgggcgt tccctgcagc gtttggggcg aagctcgcgg ttgga
//
LOCUS       DASZMB010000243        14158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_4382915, whole genome shotgun sequence.
ACCESSION   DASZMB010000243 DASZMB010000000
VERSION     DASZMB010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 14158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14158
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4382915"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VG272_11375"
     CDS             complement(<1..527)
                     /locus_tag="VG272_11375"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWF14329.1"
                     /translation="MNYYGGKELAESFRTVRNNTLAIAEDIPEDKYSFRPMPDSRSVA
                     ELLAHIAISNGFQKKIHAEDRLSTLAGFDFPSLMKRLSAEEKTSRTKAQLIELLKIDG
                     ESWAKWMEGLDDSFLGEVLTMPPGGTPPSRTRFDMILSVKEHEMHHRGQLMMIERMLG
                     IVPHLTRRMQERHAAS"
     gene            847..2934
                     /locus_tag="VG272_11380"
     CDS             847..2934
                     /locus_tag="VG272_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWF14330.1"
                     /translation="MRQRGIFFGACSFLLMLTLAIFTSGPVDAGRPSGDDADTPTPSA
                     LHDNLQRSYRTDKYLEVAESGAARGENIYWHKCWACHNQYQQAAPTLEGLFKRAALIT
                     GVSVTEENVAGHIKKGGPGMPSFGTTLSNSDVEDVVSYLHSDKCCVEGESLPANPWYR
                     SETNKWTVQTGLSGGAKGIVRVASGDSPEGIMVQLIAPNGVRTTVYTNEDGKYEFPKM
                     QGGDYTLRIANPLEFKPYKRDAVHIDSSSKLEDIVLERITKNRALPATPEVESQLSGE
                     EILWNLPGTVEEKEALHATCALGCHSFQQIFKNRYDERSWGVLVSRMLHRGGGPLIND
                     PLEPVTESQQATDHMLVKWLAKVRGPEAVDPPMFAFPRLTGESNRVVVTEFELPRALQ
                     SAHDVYGDGHGNIWYTSHLSRIFGKLDTRTGIVTEYQTPLTPGAQPGTHHVYVEKNGE
                     VLISEPWSHKLLKLDPRNGEMTEVPVAAPFPINSAGMADFDLTPDGFVWASMGGGYAA
                     EKIDPKTGKLVQKYPMKVPFSYDGIISQDGNFWAGGAISGTTGNSAELLDIRTGEMLN
                     LDSGDRKSAGRRGGFDPFGNAWFGGENGTLVELDAKAKRIREFYPPGPLDPYTDLYSV
                     EPDQNGEVWGGELHGREFLRYNPKTGHWTEYAMPEPYSHSRAVWIDKSTTPTTVWYAD
                     YSTGRVVRIQPME"
     gene            3017..3592
                     /locus_tag="VG272_11385"
     CDS             3017..3592
                     /locus_tag="VG272_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14331.1"
                     /translation="MSTMTHVPNVPQIEDQAADPGLRDVIERAKVTKSPPPAWYRTMG
                     QNPEVAKAFAGYWDMLHRGGTVPHAIKELARIQIAQMVGCEFCARQTSPLASSITEVE
                     KRSCALPNWEHPDPKTRAALHYARTLTLDDGRDKDVYEELKKFYTNAEIIELSAFFCL
                     TAGGNRMAKSWSIEAHGEVPAIPDGAMSIHS"
     gene            3889..4632
                     /locus_tag="VG272_11390"
     CDS             3889..4632
                     /locus_tag="VG272_11390"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HWF14332.1"
                     /translation="MQLVSSSEWEKIGDEIHVWHASLDRADEVICELEPLLSPEEKTR
                     ADRFHFVKDRNQFVVARGLLRELLGAYLRQAPASLAFSYAQHGKPSLAGADLSSGLSF
                     NFSHSGGMVVYAIARERNLGIDVEQMRPDAAGEGVAQRYFSAREVNDLRTLPPENRVE
                     GFFNCWTRKEAYLKATGMGLQIALDSFAVNLLPGQPAQFLSGVETKWQLAAHRPAAGY
                     AAALVYDGVPCAIKYFSADNMARQQTKIS"
     gene            4715..5821
                     /locus_tag="VG272_11395"
     CDS             4715..5821
                     /locus_tag="VG272_11395"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWF14333.1"
                     /translation="MQKVNIGIIGTGWCGGIRAETCAASPYVSELHIAEIKEARLKEV
                     AEKTHPVTATTDYRELLKNDKINAIVVSATPETVHYPMARETLLAGKHLLLEKPISLT
                     LAEADELIGLARSKSLLFTIGYSQRFNPKFAYVKRSIADGTIGQPSSALVSRHITRNL
                     GNKIGGRIKLSPAAMEATHDIDFVLWCFEPRKPVRVYAQLVNKIMHPTHGVPDCVWMI
                     VTLDDGSVFTIGAGWVLPPAYPNFSSTWIEMVGSEGAIMVDDSHRDISINTMKRGIEF
                     PLSTMPGEKVEHVYAGPMEAETIHFLDCVAHGRQPLVTPEHARMVMQVYQAADQSAEE
                     NRPIDIVWPDAGHRVAAENDFVGMRAQAPVRSKE"
     gene            5955..7298
                     /locus_tag="VG272_11400"
     CDS             5955..7298
                     /locus_tag="VG272_11400"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF14334.1"
                     /translation="MPDRNGTSASASAAIEDTSTLKPTKARWLVLVLISFMYLITYMD
                     RSNISVAAPGITKEFGLSKTAMGWVFSAFTLAYALGQFPSGWLGDRIGPRKVLTVIMF
                     WWAIAAVSTGAAVGFVTLLSARFLLGLGEAGAFPVATRGMQLWFPKVERGRIQGVTHC
                     FSRFAVAITPFVAVGIMSAFGWRAIFFIFGSLGAVWATVFGITYLNRPEDHKGVNKLE
                     LAQIRGLNVDGSIKQLETGNRRNISWKIILRSPNMWYIAAAYGCFFYGTYFFLTWYPT
                     YLQEYRHFSIRSLGFVASLPLFAGMIGDLVGGSISDLVYRKTGKLRIARRIVAVPGLV
                     ISGLMLISTALTHSAWTAVLCLAISFFFLELVIGPAWAVPMDVGGEFSGTVSSVMNTA
                     GALAASISPVVFGFFAQRGFWIAPFFVTAAVLLGGALIWSFLIDPEMSVVGGTRV"
     gene            7473..7946
                     /locus_tag="VG272_11405"
     CDS             7473..7946
                     /locus_tag="VG272_11405"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWF14335.1"
                     /translation="MTRRRLVAVTLSVFAFFAIAWRTARSAAPPDDLRDEVMKMEEER
                     NQALQKGDIATLERIYSDDLVYTNATGALLTKSQHLAELKSRGLNFRAFKHEDVQETI
                     HGDTGFVTGISKSVVEYRGSVSTGSRRFLNVFVKKDGRLRLVAHVEANIPESKSN"
     gene            complement(8056..8454)
                     /locus_tag="VG272_11410"
     CDS             complement(8056..8454)
                     /locus_tag="VG272_11410"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HWF14336.1"
                     /translation="MRNKMCLTAADTEKILAACKFEAEKNKWNVTIAVVDDGGYLVHL
                     ERMDGAPAQSALIATCKAQTSAATRTPTKTLEDIVKDRPATVAFPGRVAVQGGLPIMY
                     QGECVGAVGVSGAKSHEDEQVAAAGLKAIA"
     gene            complement(8602..10392)
                     /locus_tag="VG272_11415"
     CDS             complement(8602..10392)
                     /locus_tag="VG272_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14337.1"
                     /translation="MTKRNRAILLAGALMGVALLLWARGGQAQSQTTQTTGTGTITGT
                     VTANRDYALSEASGNHIPALVAVRVRARDNERRITYTVFTQKGHYQIYNLPVGTYQMS
                     ALQDGFDSKGPTIELKAGETKTGDVALTARPDHLRAELVDFDALFTPGPTRDYVMQNC
                     MGCHGYEHIPWQRMGGRSEEVWGNAISRMFNMDGTSPVNHTGVPQVNPAAIPEAKRQE
                     IAAYFAKIFPEGGKPRDLKLDDLKLDEAALSRTIYIEYDLPPLLPTKANPELSSHDVW
                     PSRFSPTVYVSEMGDDVIQGMDKNRIDYPGRFTTYKLPNPDQGHRVLGPHGITQAKDG
                     HVYAAEIDESSIAELDPETGKVVQYETPTRSTPHTIRSDSKGNVWFTEMQGVSKVAKL
                     DAVTKKITEWDPSPTDTNAHYYGMTVDQKDRVWAVGMTSHKIVGYDPKTNKWSVYATP
                     TQPSGPRRPTVDSKGKVWFSEHIGQALGVLDPDTGKITEYKDPFKLGGGYECYADSHD
                     NIWVTLRSYGVLARFDQKTKTYTYFPEPFPDIMGREHPGPSGTRLGGLYPPKIEEDAQ
                     GTIWYAGIRMPNITAFKPEGNVPNNQRAGN"
     gene            10578..11774
                     /locus_tag="VG272_11420"
     CDS             10578..11774
                     /locus_tag="VG272_11420"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWF14338.1"
                     /translation="MIHTGRHWLQIPGPTNVPDRILRAMDAPVIDHRSAEFANLGKEV
                     LHGVREVFKTAGSVVMYPASGTGAWEAALVNTLSPGDRVLLFETGHFSSLWRAIAEKF
                     GLQVDYVPGNWRKGASAAECEARLAVDKNHAIKAVMVVHNETSTGVTSRIPEIRRAIN
                     SAKHPALLLVDTISSLGSIDYCHDEWGVDVTIAGSQKGLMLPPGMSFNAISEKALRAA
                     ETAKLPRAYWDWQEMLKPNQTGFFPYTPPTNLLYGLKEALVMMREEGLANIFHRHDRY
                     AEATRAAVKAWGLEIVCEEPREYSSSLTAVFMPEGHNADKLRAVILEHFDMSLGAGLS
                     KFAGKVFRIGHLGSFNDLMLAGTLCGVEMGLRLAGVPHKEGGVMAALDSLAKTQNKSE
                     PVAAAR"
     gene            complement(11803..12441)
                     /locus_tag="VG272_11425"
     CDS             complement(11803..12441)
                     /locus_tag="VG272_11425"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWF14339.1"
                     /translation="MVHERLREIPVRFYRTTAGVEPVLEWLHRLDKGDRHAIGLDLMR
                     VQFGWPIGMPLMRSLKSGLWEVRSTLPSQKIARLIVCFHQNNLIVLHGFIKKPKRTPR
                     EDIDLAMRRKAGGYSVKKKKTHIGSSLNDFLKEEGILEEARATALKEALAWQVQNAMK
                     KEKINKVEMARRMKTSRAALDRLLDPGYSSVTLQTLCKAARAVGRDLRIELV"
     gene            complement(12530..13885)
                     /locus_tag="VG272_11430"
     CDS             complement(12530..13885)
                     /locus_tag="VG272_11430"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWF14340.1"
                     /translation="MEKSQSAGRSHVRWEVLLLLCLMYMITYLDRVSLANTATMISKE
                     YGFSKATMGFIFSAFIWAYALFQVPGGWLGDRFGPRRVLSVIMAYRTVIAVLTTLVSG
                     FYTLWGIRFLLGMGEAGAFPTATRAMQMWYPRDERGFVQGISHAASRFGAAIGPPIAV
                     AIMIHYGWRSVFYVIGVLSLIWSVLYLTVYRNMPEEHKSVSRTELARIRGLDEKGETK
                     KANIAKRPKVPWSVLLKHGNMWAIMCAYATYIYSLWFFLSWLPSYLIEYRHFTLIKTG
                     MFASLPLGAGVIGDAFGGWLTDKLLVKTNNPKFSRRFVAIVAMLGCATFTMFAALSAN
                     PNAAVYFLTGAMFFLEMNIGPAWAVPMDVGGEFSGTVSGMMNMAGQFVGALSPTVFGI
                     LVSRGSWVAPFAVSAGLLFIGAAIWAFWIDPEQSVIDKSQGMPGPSVGVLPAPVATAN
                     R"
BASE COUNT         3174 a         3690 c         4024 g         3270 t
ORIGIN      
        1 gaagcagcat ggcgttcctg catgcggcgc gtgagatgag gcacgatgcc cagcatgcgt
       61 tcaatcatca tcaattgacc gcgatgatgc atttcgtgtt ctttgaccga aagaatcatg
      121 tcgaaacgcg ttcgggatgg cggtgttcct cccggcggca tggtcagaac ttcacccagg
      181 aaactatcat caagcccttc catccatttg gcccaggact cgccgtcgat ctttagaagc
      241 tcaattaatt gagcttttgt gcgcgacgtt ttttcttccg cggacaagcg cttcatcaga
      301 gatggaaaat cgaacccggc aagagtgctc aggcggtctt cggcatgaat tttcttttga
      361 aagccgttgc ttatcgcgat gtgcgcaagc aattcggcta cgctccggga atcgggcatg
      421 ggacgaaaac tgtacttatc ttccggaatg tcctccgcaa tggccaatgt attgttgcgg
      481 acggtgcgaa aactttcggc taattccttg ccgccataat agttcatcga attctccctc
      541 gctccaggca tttaattttc catgaatgga aaggacataa gtttaccacg gccttccgca
      601 caatgctggc tgtgaagtgg ggcgatgccg cggaaattag ccagagcgaa attgattttg
      661 taaaacgcga ttttctgggc caaaggcaac ggttcgtcta ttcctatttc aatgataaat
      721 aataggagat cgcctcgcct gccgaaaacc gttgtagaat atggacattc ggcgtagcaa
      781 cgcgcaggct acgtctggtt acgtgggatg atttcatcct tttgttgatt tttggtcgag
      841 gtgaatatgc ggcaacgagg catctttttc ggtgcatgct cttttcttct gatgttgact
      901 ctggcgatct ttacatccgg cccggtggat gccgggcgtc cctcaggcga cgatgccgac
      961 acaccaacgc catctgcatt gcacgacaat ctacagcgct cctaccggac cgacaaatat
     1021 ctggaagtgg cggaaagcgg cgcggcgcgc ggagaaaata tttactggca caaatgctgg
     1081 gcctgccata accaatacca gcaggccgcg cccacgctcg aaggtctttt taaacgcgca
     1141 gccctaatca ccggagtttc tgtcaccgaa gaaaatgtgg ccgggcacat taaaaagggt
     1201 gggccgggga tgccgtcgtt cggcaccaca ctttccaatt cggacgtcga ggatgtcgtc
     1261 agctatttac acagtgacaa gtgctgcgtg gaaggcgaat cgcttccggc aaatccgtgg
     1321 tatcggtcgg aaacaaataa atggaccgtg cagacaggtc tttccggcgg ggccaaggga
     1381 attgtgcgcg ttgcaagcgg cgattcgccg gaaggcatca tggttcaact gatcgcgcca
     1441 aatggcgtgc gcaccacggt ctacacgaac gaagacggaa agtacgaatt tccgaagatg
     1501 caggggggtg actacacgct gcgtattgcg aatccgctgg aatttaaacc ctacaagcgt
     1561 gacgcggttc acatcgatag ttcgtcgaag ttagaagata tcgtgctcga acgcatcacc
     1621 aagaatcgcg cacttccggc tactccggaa gttgaatcgc agttgagcgg cgaagaaatt
     1681 ctttggaatc ttcccggaac ggtcgaggaa aaggaagcgc tgcacgcgac gtgcgcgctc
     1741 gggtgccact cgttccagca gattttcaag aacaggtatg acgagcgcag ttggggcgtg
     1801 cttgtgtcgc gcatgcttca tcgtggcggc ggtccgctga tcaacgatcc actcgagccg
     1861 gtgacggaat cgcagcaagc caccgatcac atgctcgtca aatggctggc gaaagttcgt
     1921 ggcccggaag cagtcgatcc gccgatgttt gcatttccgc gcttgacggg ggaatcgaat
     1981 cgcgtggtgg tcacggaatt tgagctgccc cgcgcgctgc aaagtgcgca tgacgtttac
     2041 ggcgatggcc acggaaacat ctggtacacg agccacctaa gccgaatatt cggaaagctc
     2101 gatacgcgga caggaatcgt caccgaatat caaactccgc tgacgcctgg agcacagccg
     2161 ggcacgcatc acgtgtatgt ggaaaagaat ggcgaagtac tcatttccga gccctggtcg
     2221 cacaaacttc tgaagctgga tccgcgtaat ggagaaatga ccgaggtgcc cgttgccgct
     2281 ccatttccaa tcaactccgc gggaatggct gatttcgacc tgacgccgga cggatttgtt
     2341 tgggccagca tgggcggcgg atatgccgca gaaaagatcg atccgaaaac cggaaagctc
     2401 gtgcagaaat atccgatgaa ggttccgttc agctacgacg gaattatttc gcaggacgga
     2461 aatttctggg ccggaggcgc gatttccggc acgacgggaa attcggcgga gctcctggat
     2521 atccgcacag gcgaaatgct gaacctggat tctggagatc gcaaatccgc tggccgccgc
     2581 ggcggattcg atccttttgg aaacgcatgg ttcggcggcg agaacggaac gctagtagaa
     2641 ctggatgcca aagcgaagcg catccgcgaa ttttatccgc cgggtccctt ggacccttac
     2701 acggatttgt attccgtgga gcctgatcag aacggcgaag tgtggggcgg agaactacat
     2761 ggccgcgaat tcctgaggta taacccaaag acaggccact ggacggagta cgcgatgccc
     2821 gagccgtatt cgcattcgcg cgccgtttgg atcgacaagt ccacgacgcc gacgacggtt
     2881 tggtacgcgg attacagcac gggacgcgtg gtgcgcatcc agccgatgga ataggaattt
     2941 tgctaattcc taaaatctgg ttgtaacttt tattggtgta ttggtgtccg caattagatc
     3001 gttacggaga atgcagatgt caaccatgac gcacgttccg aatgttccgc agattgaaga
     3061 tcaggcagcc gatccgggat tgcgcgacgt gatcgaacgg gcgaaggtga cgaagagccc
     3121 gccgcctgcc tggtaccgta cgatggggca gaacccggaa gtggcaaagg cgtttgcagg
     3181 ctactgggac atgcttcatc gcggtggcac agtgccgcac gcaattaagg aactcgcgcg
     3241 cattcagatt gcgcaaatgg taggctgcga attctgcgcg cggcaaacct ctccgctcgc
     3301 ttcatcgatc acggaagtgg aaaaaagaag ctgcgccctg ccgaactggg agcacccgga
     3361 tccgaagacg cgcgccgctt tgcattatgc acgcacactt acgctcgatg acggcaggga
     3421 taaagatgtg tacgaagaac tgaaaaagtt ttacaccaat gcggaaatca tagagctgtc
     3481 ggcattcttc tgcctgacgg ccggcggtaa tcgcatggcg aaaagctgga gcatcgaagc
     3541 gcatggcgaa gtgcccgcga tacccgatgg ggccatgtcg attcacagct agctggcagg
     3601 aataaatcga aaaccaggat cggtttcagt ggcgcgggct tcagagtctg tgtgagaact
     3661 tcagaatctt tgtgggtcgt ggctttagcc acgacataag tcctgcaaaa tgagtgcggc
     3721 tttagccgct gaagttccaa ttttgcagtt ctcacacaga ctcttcagcc tgcggacttt
     3781 tgtccttgca agggctaaac tccgcagggc tgaagcctgc gccacaaaag cacaaagtca
     3841 tccaacatgc tcggtagcga gccgttggct cacaggcaaa ggggaactgt gcagctggtt
     3901 tcttcatcgg aatgggaaaa aatcggcgac gaaatccacg tgtggcatgc atcgctcgac
     3961 cgcgcggatg aagttatttg cgaattggag ccgcttcttt cgccggaaga aaaaacacgc
     4021 gcggaccggt ttcatttcgt aaaggacagg aatcagtttg tcgtagcgcg cggactactt
     4081 cgtgaattgc ttggagcgta tcttcgacaa gctccggcaa gcctcgcgtt ttcctacgcg
     4141 cagcatggaa agccttctct tgctggggct gatttatcga gtggactgag ttttaatttc
     4201 tcgcactctg gcggcatggt tgtgtatgcg attgccaggg agcgcaatct tggcattgac
     4261 gtggagcaga tgcggcccga cgcggcaggc gaaggtgttg cgcagcgcta tttttccgct
     4321 cgcgaagtaa atgacctgcg aacgttgccg ccggaaaaca gggtcgaagg atttttcaat
     4381 tgctggacgc ggaaagaagc ctatttgaag gccacgggaa tgggattgca aatcgctctc
     4441 gatagttttg ccgtaaatct gttgcccggc cagcccgcgc agtttctcag cggcgtggaa
     4501 accaaatggc aattggccgc gcaccgtcct gctgcaggct acgcggcggc gctggtctat
     4561 gatggcgtgc cttgcgccat caagtatttt tccgctgata atatggcgcg tcagcaaacc
     4621 aaaatcagct aacatatggg attcgcgcgg ctgtgccggc ccaaagaatg aatacacttg
     4681 attctaaatt gcagaatttc tgagtggagg aaatatgcag aaggtaaaca tcggaatcat
     4741 cggaacagga tggtgcggag gcattcgcgc ggaaacctgc gccgcgagcc cctacgtgag
     4801 cgaattgcac atcgccgaaa ttaaagaggc gcgcctgaag gaagtggctg agaagacgca
     4861 cccagtgaca gcgaccacgg attaccgtga actcctgaag aacgacaaga tcaacgccat
     4921 cgtcgtttca gcgacgcccg aaacggttca ctatccgatg gccagggaaa cgctgctcgc
     4981 gggaaaacat cttttactgg agaagccgat ctcgttaacg ctggctgaag cggatgaact
     5041 gatcggtctt gcgcggtcga agtctttgct tttcacgatc ggatattcac agcgcttcaa
     5101 tcctaaattc gcatatgtaa aacgttcgat cgcggatggc acgattggtc agccgtccag
     5161 cgcgctcgta agcaggcaca tcacgcgcaa cctgggcaac aagatcggcg ggcgcatcaa
     5221 gctttcgcca gcggcgatgg aggcgacgca cgatattgat tttgtgctgt ggtgcttcga
     5281 gccccgcaaa cctgtacgag tttacgcgca gctggtgaac aagatcatgc acccgacgca
     5341 cggcgtgccg gattgcgtct ggatgatcgt aacgctcgat gacggcagcg tgttcacgat
     5401 cggcgcgggc tgggtgttgc ctccggcata cccgaatttt tcatccacgt ggatcgagat
     5461 ggtcggcagc gaaggcgcga tcatggtgga cgattcgcac cgcgacatca gcatcaacac
     5521 gatgaagcgc ggcatcgaat ttccgctctc gacgatgccc ggcgaaaaag tggaacacgt
     5581 gtatgcgggc ccgatggaag ccgagacaat tcacttcctg gattgcgtgg cgcacggaag
     5641 acagccgctc gttacgcctg agcatgcgcg catggtgatg caggtgtatc aggcggcgga
     5701 tcaatccgcg gaggaaaatc gtccgattga cattgtgtgg ccagacgcgg gccaccgcgt
     5761 tgcagcggaa aatgattttg ttggaatgcg cgcgcaggcg ccggtgcgaa gcaaggaata
     5821 atttaaaagc gccaacgaat ttgattgatg tagcgcccgc cttcaggcgg gcaatttagg
     5881 tcacgaccaa aatgcccacc tgaaggtggg cgctacagat cgactatctg ttatacaaaa
     5941 cgacgggggc aagaatgccg gacaggaatg gcacgtccgc ctcagcaagc gccgccatcg
     6001 aagatacttc cacactcaaa cccacgaagg cccgctggct cgtactcgtt ctgatcagct
     6061 tcatgtactt gatcacgtac atggaccgca gcaacatttc cgttgccgct ccgggcatca
     6121 ccaaagaatt tggattgagc aagacagcca tgggctgggt tttcagcgcg tttacgttgg
     6181 cctatgcgct cggccagttt cccagcggat ggttgggaga tcgcattggc cctcgaaaag
     6241 ttttaacagt catcatgttc tggtgggcga tcgccgcggt ttccacgggt gccgccgttg
     6301 gatttgttac tttgctaagc gcgcggtttc tgcttgggct tggagaggct ggcgcatttc
     6361 ctgtggccac acgcggcatg cagctttggt ttccaaaagt agagcgcggc cgcattcagg
     6421 gcgtcacaca ttgcttcagc cggtttgcag tggcgatcac gccattcgtg gccgtcggca
     6481 ttatgagcgc attcggatgg cgcgccatat tttttatttt tggatcgctg ggcgcggttt
     6541 gggccactgt ttttggcatc acttatctaa atcgtcctga agatcataaa ggcgtcaaca
     6601 aattggagct ggcgcaaatt cgcggcctca acgtggatgg ctcgatcaag caattggaga
     6661 ccggtaatcg gcggaacatt tcgtggaaaa taattttgcg ttcgccgaac atgtggtaca
     6721 tcgccgcggc atacggttgt tttttttacg ggacctattt ttttctgacc tggtatccga
     6781 cctatttgca ggagtaccgc cacttctcca ttcgttcgct gggctttgtc gcttcgctgc
     6841 ctcttttcgc tggaatgatt ggcgatctgg tcggaggatc gatcagcgac ttggtttatc
     6901 ggaagacagg aaagctcagg atcgcccgcc gcattgtggc agttcccggg ctggttattt
     6961 ccggtctcat gctgatttcg acggcgctca cacacagcgc ctggacggca gtactgtgcc
     7021 tcgccatttc ttttttcttc ctggaactgg tgattggtcc ggcgtgggcc gtgcccatgg
     7081 acgtgggcgg agagttcagc ggcacggtga gcagcgtgat gaacacggca ggtgcactgg
     7141 cggcgtcgat ttccccggtc gtgttcggat tttttgcgca acggggattc tggatcgcgc
     7201 cattctttgt taccgccgcc gtgttgctgg gcggcgcgct catctggtca tttttaatcg
     7261 acccggagat gtcggtggtc gggggaacac gcgtctagcg cggcgaaggc gattgtcttc
     7321 ctacgggcag ctcttttttc catgcaattc taaacgattg actaccgcca acggctctat
     7381 cttgacgctc acggcgagtg cgtgctatca cggttcagcg ggaattcttc aacgaagtca
     7441 gatccgatcc gagaaatggg agaaataata agatgacgcg gcgacgattg gttgcggtaa
     7501 cactgtcggt ctttgcattt tttgcaatcg catggcgaac agccaggagc gctgcgcctc
     7561 ccgatgatct ccgggatgaa gtgatgaaaa tggaagaaga gcggaaccag gcgctgcaaa
     7621 agggcgacat cgcgacgctg gagcgcattt attcggacga tctggtgtac acaaacgcca
     7681 ccggcgcgct gctgaccaag tcgcagcacc tggccgagct taagagtaga ggtctgaatt
     7741 ttcgcgcgtt caagcatgag gatgtgcaag aaaccattca tggcgatact ggattcgtga
     7801 ccggcattag caagtccgtg gtggaatatc gcgggtcggt ttccacagga tcgcgcagat
     7861 ttttgaatgt ttttgtgaaa aaagacgggc gattgcggct cgtggcgcat gtggaagcga
     7921 atatcccgga gtcgaaatcg aattaagaag attgaaaaaa gcggcgcggg cctcagcctg
     7981 cggagttttg tccttgcacg gactgacttc cgcaagctga agcccgcgcc attttacagc
     8041 cgaatattta tttggttagg cgattgcttt caggcccgcg gcggcaacct gctcgtcctc
     8101 gtgcgactta gcgccggaaa cgcctacggc gccgacacat tcgccttgat acatgatcgg
     8161 caatccgccc tgaacggcta ctcggccggg gaatgcaacc gtcgccggtc gatccttcac
     8221 gatatcttcc agagttttcg ttggagttcg tgtcgctgca gatgtttgcg ctttgcacgt
     8281 ggcgattaac gcgctctgcg ccggtgcgcc atccatgcgt tccagatgaa ccagatatcc
     8341 gccatcgtcc acgacggcaa ttgtgacgtt ccacttattc ttttctgcct cgaacttgca
     8401 tgcggccagg attttttccg tgtcagctgc cgtcaaacac attttgttac gcactgtttc
     8461 tccttgtatg aatcttgttt tgatttattg cggacctgcg cgtaggtcag gacccggcat
     8521 tcgaaattta ctctaccttt cgaatgccag acccgcataa cgcttcgata cgctgtacgg
     8581 atgattcttg cttgaaccgg cttagttgcc cgcccgttgg ttgttcggaa cgtttccttc
     8641 gggtttaaat gccgtaatgt tgggcatacg gatacccgcg taccagatcg tgccctgggc
     8701 atcttcttca atcttgggcg gatacaggcc gccgagacgt gtgccactgg ggcccgggtg
     8761 ttcacgcccc atgatgtcgg ggaacggttc cgggaagtag gtgtaagtct tggtcttctg
     8821 atcaaaccgg gccagaacgc catagcttcg gagggtcacc cagatgttgt cgtgggagtc
     8881 ggcatagcat tcatatccgc caccgagctt aaatggatct ttatactcgg tgattttgcc
     8941 cgtgtcgggg tcgagcacgc cgagcgcctg tccaatgtgc tccgagaacc acaccttgcc
     9001 cttggaatcg acggttgggc ggcgcggtcc ggaaggttgc gtaggagttg catagacgga
     9061 ccatttgttg gtcttcgggt catagcctac gatcttgtgt gaggtcatgc cgacggccca
     9121 cacgcggtcc ttttgatcaa cggtcatgcc gtagtagtgg gcattcgtgt ccgttggtga
     9181 aggatcccat tcggtgatct tctttgtcac ggcgtccagc ttggcgactt tgcttacgcc
     9241 ctgcatctct gtaaaccaca cattgccttt ggaatccgag cggattgtgt gtggagtgct
     9301 gcgggtcgga gtttcgtatt gaacgacttt tccggtttcg gggtccaatt cagcgattga
     9361 actttcgtca atttcagctg cgtacacatg gccgtccttg gcttgggtga ttccgtgcgg
     9421 tccgaggacg cggtggccct gatcgggatt cgggagcttg taggtagtaa atcggccggg
     9481 ataatcgatc cggttcttgt ccatgccctg aatcacatcg tcacccattt cagatacata
     9541 gacggttggg gagaagcggc tcggccaaac gtcgtggcta gaaagttctg ggttagcctt
     9601 ggttggaagg aggggaggta agtcgtattc aatataaatt gtccgggaaa gcgcggcttc
     9661 gtccagcttc agatcgtcca gcttcaaatc gcggggcttt ccgccttccg ggaaaatctt
     9721 ggcaaaatag gccgcgattt cttgcctttt cgcttccgga atagctgcgg ggtttacctg
     9781 aggcacacca gtgtggttca ccgggctggt gccatccatg ttaaacatgc gactgatggc
     9841 gttgccccag acttcttcgc tgcggccacc catgcgttgc caggggatgt gctcataacc
     9901 gtggcagccc atgcagtttt gcatgacgta gtcgcgggtg gggccgggag tgaagagcgc
     9961 gtcaaaatcg acgagttcag cacgtaggtg atcggggcga gctgttaaag ccacatcgcc
    10021 ggttttcgtc tcgccggcct tcagctcaat cgtggggccc ttggaatcga atccatcctg
    10081 cagtgcggac atctggtagg tgccgacagg gaggttgtaa atctgatagt gccccttctg
    10141 ggtaaagacc gtgtaggtga tgcggcgttc gttgtcacgt gctctgacac gtaccgctac
    10201 cagcgccgga atatgatttc ccgaggcctc gctcaaagcg taatccctgt tggctgttac
    10261 agttccggta atcgtgccgg ttccggttgt ctgcgtggtt tgcgactgcg cctgcccgcc
    10321 cctcgcccac aagagaagcg ctacacccat taaagctcca gctaatagta tggctctatt
    10381 ccgcttagtc atgaatgact cccattccca atttaatgcc cacgcccgat tcctcatagc
    10441 aaaggaattc ctgcaatttt aactttctta attcgtacac cacaaacaca tggatagtca
    10501 actcgaagta tttgaggcgg ttgcactttg ccttgttcaa catgtacaat tttgattcac
    10561 tttcatacgg agatgccatg attcatacag gacgccactg gttacagatt ccaggcccca
    10621 cgaacgtacc ggaccgcatt ttgcgagcga tggacgcgcc ggtaatcgac catcgaagcg
    10681 ctgaattcgc caatctcggc aaggaagtcc ttcacggagt tcgggaagtt ttcaaaacgg
    10741 cgggttctgt ggtcatgtac ccggcctccg gcacaggagc ttgggaggca gcgctggtga
    10801 acacgctatc tcctggggac cgtgtattac tatttgagac aggtcacttt tcttcactgt
    10861 ggcgggcgat agcggagaaa ttcgggcttc aggtggatta cgtgcccgga aattggcgaa
    10921 aaggagcgag cgctgccgag tgcgaggcgc gtctggctgt cgataagaat cacgcgatta
    10981 aggccgtgat ggttgtgcac aacgagacct ctactggcgt aaccagccgc atcccggaga
    11041 ttcgccgcgc catcaattcc gcgaaacatc ccgcgctgct tctggtggat acgatctctt
    11101 cgctcggctc gatcgactat tgccatgacg aatggggagt ggacgtcacg attgccggct
    11161 cacaaaaagg cttaatgctt ccgccgggca tgagttttaa cgcgatttcg gaaaaagcgc
    11221 tgcgcgcggc cgaaactgcc aagttgccgc gggcgtattg ggactggcaa gaaatgctga
    11281 agcccaatca gacaggattt ttcccgtata ctccgcccac caatcttctg tacggtttga
    11341 aggaagctct ggtgatgatg cgggaagagg ggctggctaa tatttttcac cgccatgacc
    11401 ggtacgcgga agcgacacgc gcagcggtga aagcctgggg tctggaaatt gtttgcgaag
    11461 aaccgcgtga atattccagc tcgctgacgg ccgtgtttat gcccgaaggg cataacgcgg
    11521 acaaactgcg cgccgtaatt ctcgaacatt tcgatatgtc cctcggcgca gggctttcca
    11581 agtttgccgg aaaagttttc cggattggac acctcggcag cttcaacgat ttgatgctgg
    11641 ctggaacgct gtgcggcgtg gaaatgggct tgcggttggc aggcgtgccg cataaagaag
    11701 gtggcgtgat ggcagctctc gattcactcg ccaagactca gaacaaatcc gagcccgtcg
    11761 cagcagcgcg ataatttttt aaagagatgg aagagttggc tgtcaaacaa gttcaattcg
    11821 taaatctcgt ccgacggctc tggccgcttt acataaagtt tgcaatgtga ccgacgagta
    11881 gccagggtca agcaatcgat caagggcggc acggcttgtt ttcatacgcc gtgccatctc
    11941 taccttatta attttttcct tcttcattgc attctgaact tgccaagcga gcgcttcttt
    12001 tagcgctgtg gcgcgtgcct cttcaagaat tccttcctct ttcaagaaat cattcaggga
    12061 tgatccgatg tgggttttct tctttttcac gctataacct cccgccttgc ggcgcatcgc
    12121 caaatcgata tcctcacgag gtgtccgttt gggttttttt atgaatccgt gaaggacgat
    12181 taaattgttt tggtgaaaac acacgatcaa gcgtgcaatc ttctggcttg gcaacgttga
    12241 gcggacttcc catagaccac tttttagact acgcatgaga ggcataccga tcggccatcc
    12301 gaattgcact cgcataaggt caagcccgat tgcgtgcctg tctcctttgt ccaaccgatg
    12361 cagccattcg agaactggtt caacgccggc ggtggttcga tagaaacgga caggaatttc
    12421 acgcagacgc tcatgaacca tgacacctca cagtcccagt atgtaccaac attgatacac
    12481 aatccagccc aatgatccgg gcgcgtgcgc caaccgcaaa acttcacttc taccggttcg
    12541 cggttgcgac aggtgcggga agtacgccga ctgaagggcc gggcattccc tggctcttat
    12601 cgatgacgga ttgttcggga tcgatccaga aagcccagat cgcagcgccg atgaagagca
    12661 ggccggcgga aacggcaaac ggtgcgaccc acgatcctct cgacacaagt atgccgaaga
    12721 cggtgggcga cagcgcgccg acaaattgac cggccatgtt catcatgccg gaaaccgtgc
    12781 ctgagaattc tccgccgaca tccattggaa cggcccaggc aggaccgata ttcatttcga
    12841 ggaaaaacat agcgcccgtc aggaaataga cagccgcatt gggattcgcc gacaaggctg
    12901 cgaacatagt gaaggtagcg cagccgagca tggcgacgat agccacgaag cggcgggaaa
    12961 acttgggatt gtttgtcttt acaagaagtt tgtccgtcag ccagccgccg aacgcgtcac
    13021 caatgactcc tgcgcctaac ggcagtgacg caaacattcc cgtcttgatc agagtgaagt
    13081 ggcggtactc aatcaagtaa gacggcagcc aggaaagaaa aaaccagaga gaataaatgt
    13141 acgtggcata cgcgcacata atggcccaca tatttccatg ctttagcagg acgctccagg
    13201 gtactttcgg acgcttggca atattggctt ttttagtttc gcccttttca tcgaggccgc
    13261 ggatgcgggc aagctcggtg cggctgacac tcttatgttc ttccggcatg ttgcgataca
    13321 cggtcaaata gaggacggac cagatcaagc tgagcacgcc aatcacgtaa aacaccgagc
    13381 gccagccata gtgaatcata atggctacgg cgatcggggg accgattgcc gcgccaaagc
    13441 ggctggccgc gtggctgatt ccttgcacga agccgcgttc atcccgcgga taccacattt
    13501 gcatggcacg tgtagcggta gggaatgcgc cggcttcgcc catgccgagc aaaaagcgaa
    13561 tgccccataa agtatagaaa ccactgacca acgtggtgag gacggcgatt acggtgcgat
    13621 aggccatgat gactgaaagc actcggcgcg gtccaaagcg gtcacccagc cagccaccgg
    13681 gcacctgaaa aagtgcatag gcccagataa acgcgctgaa aatgaatccc attgtggctt
    13741 tgctgaaacc gtattccttg ctgatcattg tggctgtatt ggcgaggctt acgcggtcca
    13801 gatacgtaat catgtacatc aggcacagga gaagcagcac ctcccaacgc acatggcttc
    13861 gcccagctga ctgcgacttc tccattacca ttcctccctt gccccgtgga aaatcgaacc
    13921 tattataatg aacgtagtct ttcagatata cactgcccca tcccttcgca gcaatccttt
    13981 tcctatattt tcctatatag gatgcagtaa gtttcgcccg ctttgtgacg ctggaattgt
    14041 agctcgcttc gcgtgtcctg agtacacgag agatcagagg cgagggttta ggtcgaagtg
    14101 tgacccaaaa tttcttgttc cgctcgtgcg cctggggctc taggccgcgg ctcaacct
//
LOCUS       DASZMB010000244        14139 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2992157, whole genome shotgun sequence.
ACCESSION   DASZMB010000244 DASZMB010000000
VERSION     DASZMB010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 14139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14139
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2992157"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VG272_11435"
     CDS             <1..569
                     /locus_tag="VG272_11435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HWF14341.1"
                     /translation="LQEDAKNCKACDLWKHATQTVFGEGSPKAKIMMIGEQPGDQEDK
                     EGRPFVGPAGKLLDAALAEAGIDRKKVYVTNAVKHFKWEPRGKRRIHKKPNTVEIAAC
                     RQWLDAEIAVVRPEVIVCLGATAAQALLGRAFRVTEQRGEFMKSELAPHIMATVHPSS
                     ILRAPDEKARHDAMKYFVADLKKIVHLL"
     gene            complement(760..1236)
                     /locus_tag="VG272_11440"
     CDS             complement(760..1236)
                     /locus_tag="VG272_11440"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HWF14342.1"
                     /translation="MRLVSSGFQDGKSIPKKFTCEGENISPEFHWENAPKVAKSFVLV
                     LHDPDAPMKDGFTHWLLYDIPASVSEIHENVPKGHERIAGLGIQGKNDSGNLGYTGPC
                     PPSGSHRYFARLYALRAELGLSPGAVLQEVRSAIGPYVIEQTEIMGTYAKARAQTP"
     gene            complement(1621..1818)
                     /locus_tag="VG272_11445"
     CDS             complement(1621..1818)
                     /locus_tag="VG272_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14343.1"
                     /translation="MAAGGVTKCLHPPCNCNVEIEERYCSEACASAIDQPLVPCPCGH
                     PECVGVEDAVDREELDDVPTT"
     gene            complement(1871..2176)
                     /locus_tag="VG272_11450"
     CDS             complement(1871..2176)
                     /locus_tag="VG272_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14344.1"
                     /translation="MVSKAASIPNYKVVEERKRRELEVCVAALKSSDVWRQFEALYRN
                     LYASAELAGVRKPPLEELLGLGEPEKGTCALEQKEVSHLTSEDQQETEIAAELEKGS"
     gene            complement(2882..3784)
                     /locus_tag="VG272_11455"
     CDS             complement(2882..3784)
                     /locus_tag="VG272_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14345.1"
                     /translation="MRLIRATVCLFLLVGVVCACEMVASAGQPQEAPGETNVAKGRRI
                     TKQVIAALGGPAYLNVQNVQCDGRDATFGTAGTLMGFTLFRDTWLLPDKNRVEYFTKG
                     EHTIVGFLMGSDDLLITHGGAMITVFNGKEGWTLDKSGVSNQPDDLIQNFNDQLKTDM
                     NNMLRKRINEPGVEAEYAGTDLIDLKEAEWIEFTDREHRMMRLGIDKYSHLPLRWVVV
                     TRNPETRERAEVITSLTQYALNDGVKTPLSIELSRNDRKVSQTFLSSCKYNSDMSPEL
                     FTRASLEQAAKDTNKKGYKESKSR"
     gene            complement(3967..4413)
                     /gene="rplI"
                     /locus_tag="VG272_11460"
     CDS             complement(3967..4413)
                     /gene="rplI"
                     /locus_tag="VG272_11460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HWF14346.1"
                     /translation="MQIILQEDVEKLGTRGEVVEVAAGYARNYLLPHKLALEASPANI
                     KRLEKIRVTLAKLTATEKEAAHQQAALLNQVTVKFTRKAGETDQLFGSVTSADLADAL
                     KAQGFEIDKRRIQLDEPIKTLGEKPVTVKLVHGVTAEFKAVIEKEA"
     gene            complement(4592..5068)
                     /gene="rpsR"
                     /locus_tag="VG272_11465"
     CDS             complement(4592..5068)
                     /gene="rpsR"
                     /locus_tag="VG272_11465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HWF14347.1"
                     /translation="MMSDQPVPSAPQEPHAPSATPTPASAPAHRAEGHTGGPPRRSEG
                     HSGGGHSGGGHSGGGGGRREGGPGGSGGPGGGPGGPRRGGKRQYFRKKKVCRFCVEHV
                     DFIDYKKADQLQPFVQERGKILPRRMTGTCSRHQRWLGEAIKRARNIALLPFATEL"
     gene            complement(5108..5536)
                     /gene="rpsF"
                     /locus_tag="VG272_11470"
     CDS             complement(5108..5536)
                     /gene="rpsF"
                     /locus_tag="VG272_11470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HWF14348.1"
                     /translation="MEERIYDLIFICRPATPEEEINKIVTTLEHSTGEHGGKIEKTER
                     WGTRRLAYRVAKHREGFFVYMVVKSTQGDLIKELERRLKVSDAVIKYQTIRLDEELKR
                     QQKLGNHRERRASRRPRKQAAAAPAAPQPQQQQAPAPAAE"
     gene            complement(5691..6293)
                     /gene="pth"
                     /locus_tag="VG272_11475"
     CDS             complement(5691..6293)
                     /gene="pth"
                     /locus_tag="VG272_11475"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HWF14349.1"
                     /translation="MRVIVGLGNPGIEYAWTPHNLGFLVVDALAEDAGIRVSRPESKS
                     LVGLGEIAGQDVVLAKPQTMMNLSGLAVRELVGRAECEPSDVIVLCDDIALPWGMLRV
                     RERGTAGGHNGLKSVIGALGTVEFVRVRLGVKPEEMRGDLREYVLRQIRRDEEDLVAE
                     EIEQGAEAVKAILAEGTQAAMNRFNRRVSPDENNGEQNES"
     gene            complement(6381..7085)
                     /locus_tag="VG272_11480"
     CDS             complement(6381..7085)
                     /locus_tag="VG272_11480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HWF14350.1"
                     /translation="MEQIIVEAAPRESRGKNAARRLRVAGTVPAVLYGGKGGPQALQV
                     NTKHVSAILRSATGHNTILTVKTEGGEDFAIVKEYQVDPIKGTLLHVDLLRVAMDVRM
                     RIKVPVHTFGEPQGVKLQGGVFEVVTREIEIECLPVDIPTEFKVDISNLMMGMQIHAS
                     DIPLDPQKMKLMTDPLRVLAHVVALRVEEEKAPEAVAGEAAAAPAEPELIKKGKKDEE
                     GEEGEAAAPAEKEKKK"
     gene            complement(7175..8119)
                     /locus_tag="VG272_11485"
     CDS             complement(7175..8119)
                     /locus_tag="VG272_11485"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HWF14351.1"
                     /translation="MTDDKFKIFCGSANPRLAADICRHLNVKLGDAKLGKFSDGEVYF
                     QILENVRGADVFVVQPCSNPSDFHLMELLMMIDAFKRASAWRITAVVPYYCYARQDRK
                     DKPRVPISAKLVADLMETAGANRALTLDLHAPQIQGYFTVPVDHLYASPVLIEHFMQK
                     KMGNLTVVSPDAGGVERARAFAKKLDSPLAIVDKRRVDIDVSEVMHLIGDVRGRSTLI
                     IDDIIDTAGTLVKTAEALLKEGATKVYAACTHPVLSGPAVQRICASQIEEVIVTDSVP
                     LSEEAKKVSKIKVLSVADLLARGIHSIHEETSISELFY"
     gene            complement(8147..9058)
                     /gene="ispE"
                     /locus_tag="VG272_11490"
     CDS             complement(8147..9058)
                     /gene="ispE"
                     /locus_tag="VG272_11490"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HWF14352.1"
                     /translation="MKSVRIPAYAKVNLRLAVLGRRADGYHELRTIFQTISLHDTLVL
                     ESKREPGVDLCVDGNSQLAGEPGKNNLVYRALDVLSRELGLKQGVRALLTKRIPVGRG
                     MGGGSSDAAAALVGLLRLAGKRIPLERLMTIASGLGSDVPFFLQGGRALGIGRGEEIY
                     PLPDVPKRTLLMVSPHEIAVPTKDAYRWISEAQSEELTNREDPSRLLQFCALCWSSQG
                     GTLSNDFEAAVFPRYPRLDAIKRELLQQGAAEASLAGSGSAVFGIYQHPAMARRAARA
                     FPQDQVFLCSTLSRVEYRQKMGAILSK"
     gene            complement(9074..11164)
                     /locus_tag="VG272_11495"
     CDS             complement(9074..11164)
                     /locus_tag="VG272_11495"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF14353.1"
                     /translation="MKIARHLAVLFIFSLAGGSALCAQQGKRVPSAQTSAPVSDLDSG
                     IAAQADAYDNFVMGHYYSQEFQSSSHAEDANKAIDFLKRAFTLDPNSQQIGDELAEIY
                     YQSQRIRDAVTEANSILAKDPDNLPARRLLARIYVRTLGDLSNASGQRDTITRAVEQY
                     REIVRLDPNDSDAELWLARLYRLQNEQEKAEVVLRALLAREPANENGVEQLTQLLLDE
                     GKSEEAVSSLQVILQRAPTPRLWELLGDAYNQIHDLPNAEQAYRKASESQPGDLNHHK
                     ELAQTLLAEEKFPEALEQYQGLVEMDADDGDNYLRLAEIYRQMKQLDKAEQNILLAKQ
                     RTPNNLEVIYYEATIYEDEGRIDDSIRVLSEAVTAVKTESEFAPSRRRTLAILYQQLG
                     QLYRESSNYSAAVNTFEEMLRLGTEEDRRARILIVNTYRDARDISKALDAAHKAVEAY
                     PKDHSIRIAQALLLGENAQADEAVSQLRGLLDGSPSDFEVQLDIAQVYQESKRWNDAE
                     TAIHAAEKLQGDSQSKEMTGFLMGAIFERQKKFDQAETEFRKVLELNPRNSSALNYYG
                     YMLADRGVRLDEATSLIKRALVEEPGSAAYKDSLGWAYYKQDKLSEAEDMLRQALEHE
                     PHDPTILSHLGDIYAKMGKESLAEAQWQKSVDEWHRALPAEFEPAKMAEVEQKISALK
                     HRLAQQKQPGNLKP"
     gene            complement(11291..12859)
                     /gene="purH"
                     /locus_tag="VG272_11500"
     CDS             complement(11291..12859)
                     /gene="purH"
                     /locus_tag="VG272_11500"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF002049.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HWF14354.1"
                     /translation="MQRALVSVFDKSGLTEFVRQLVACGIEIVSTGGTAKLLRDDGIT
                     VRDVSELTGWPEMLGGRVKTLHPKVHGGILFRRKEAGDLQQTAEHKIAPIDLVIVNLY
                     PFEATAAKPDLTSEDLIENIDIGGPTMVRSAAKNFESVAVVTDPADYAAIADEIKAHG
                     EVSLATRLELARKAFATTARYDGKIATELERLSASTGSIELAERPLLPERLHFAYARR
                     QELRYGENPHQRAALYVPAGIHASGLAAARQLQGKELSYNNFVDLEAALNLAMEFQRP
                     AAVIVKHNNPCGTAEQETLVDAYVKALACDPISAFGGVMAFNRVVDAATGEEVAKLFV
                     ECIVAPGYEPAALEKFATKKNLRLLHLPAREGSSRDLGLELKRISGGILVQEQDAHEL
                     AETHLKVVTRRAPTREEIEALLFGWKVCKHVKSNAIVFARAGQTVGVGAGQMSRVDSV
                     KIAVMKAQLPLAGSVVASDAFFPFPDGLEEAGKAGATAVIQPGGSVRDGDVIAAADQL
                     GMAMVFCGVRHFRH"
     gene            complement(13587..>14139)
                     /locus_tag="VG272_11505"
     CDS             complement(13587..>14139)
                     /locus_tag="VG272_11505"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWF14355.1"
                     /translation="QMLSHAIQHKRIRFVEGQAEQMPFANAEFGLMTVGLAFHWFDQR
                     KFLLEAHRLLRPEGWLIIYNDVFPGQMKGNDDCEKWYHEEYLARYPTPPRNVQPLAEM
                     NASEYGIARYGFEEFTHEIEFSSEQLVNYLVTQTNVISAVEAGSEDLQSVANWLFDSI
                     RPMFSGAKETFSFLCQIQFFKRS"
BASE COUNT         2992 a         4084 c         3674 g         3389 t
ORIGIN      
        1 atctgcagga agacgcaaaa aattgcaagg cgtgcgattt atggaagcat gccacgcaaa
       61 ccgttttcgg cgaaggctcg ccgaaagcga aaattatgat gattggcgag cagcctggcg
      121 atcaagaaga caaggagggc cggccattcg ttggaccggc agggaaatta ctagacgcag
      181 ctctggcgga agcaggaatc gaccgcaaaa aagtatacgt cacgaatgca gtaaaacatt
      241 tcaagtggga accgcgcggc aagcgacgca tccacaagaa acccaacacc gttgaaattg
      301 ctgcctgcag gcaatggttg gatgctgaaa tcgcggttgt gcgtcccgaa gtgatcgttt
      361 gtctaggagc aacagcggcg caggcattgc tcggccgggc gtttcgcgtc acggagcagc
      421 gaggggaatt catgaagtcc gaactcgcgc cccatattat ggctacagtg cacccatcct
      481 cgatcttgcg tgcgccggat gaaaaggcgc gccacgatgc aatgaaatac tttgtggctg
      541 acctcaagaa aatcgtccac ttactctgaa ccggacataa acgtgccagg actagacagg
      601 cgcagggcaa agtgcgcatc ggacccttcg cgtcaatcat attccccgca cataatttcg
      661 ggcagaacat ccaatgtgaa tcgaacgtga aggccggctg catcaaaaca gtcggccttc
      721 atttatttgg atcggcgcgt cgtttgcagg aacgtaaagt cagggggtct gtgcgcgagc
      781 cttggcgtac gtgcccataa tttccgtttg ttcgatcacg tatggtccga tcgcagatcg
      841 gacttcttgt aagacagcgc ctgggctgag gcccaattcc gcacgaagtg catacagccg
      901 tgcaaaatag cgatgggatc ctgaaggcgg gcagggccct gtgtagccca ggttaccgga
      961 gtcattcttt ccctgtatcc ccaatccggc aatacgttcg tgccccttgg gaacattttc
     1021 gtgaatctcg ctcacggacg ccggaatatc gtagagcagc cagtgcgtaa atccatcttt
     1081 cataggcgcg tccggatcgt gcagaactaa tacaaaggat tttgccactt tcggggcatt
     1141 ctcccaatgg aactcgggag aaatattttc tccctcacat gtgaatttct tcggtatgga
     1201 tttgccatcc tgaaagccgg aactgacgag tcgcatgttt catctccatt gcgtggtgtc
     1261 aaaagcatgg ctggatctta acgatcgatc gaagtcctcg gggcgagatc gcgggatttc
     1321 acttcacgtc gccgtctgca cgcatagtcc aaaagctggc gccggatcaa cttaaaagca
     1381 ttggtagtgc ctcaatttgc gagcaaagtc gccctctccg tcccgaagat cacggttcac
     1441 cgagaacgtt ctccgtgaac gggacggaga ggccgctaca aaaccgcagt gcatagcagt
     1501 ttgcttttgt agaggccgcc cgtcttttcg ggcgggcagc cttttttgcc gtagcactgc
     1561 aaaattcaaa ttgatgcact accaaagcat tcccaatcgc tcggcaacaa ggaaatcact
     1621 tcatgtcgta ggcacatcgt cgagttcctc gcggtctaca gcatcttcca cacccacgca
     1681 ctcaggatgc ccacagggac aaggaacaag cggttgatcg atcgcgcttg cgcaggcctc
     1741 actgcaatag cgttcttcga tctcgacgtt gcaattgcat ggcggatgca aacacttggt
     1801 gacgccaccg gcggccatat agttccttct ttttcgttat tcgctatgtt ttattcttta
     1861 ctttgagcgt tcatgatcct ttttctaact cagcggcaat ttctgtttct tgctggtcct
     1921 ccgatgttag atgggacact tccttctgct ccagcgcaca cgttcccttc tcaggttcgc
     1981 ccaatcccag gagttcttcc aaagggggct ttcggacacc cgcgagttcc gcgcttgcat
     2041 acagatttcg atacagtgct tcaaattgcc gccaaacatc gctgctcttc aatgcggcga
     2101 cgcagacctc aagttcgcgg cgcttgcgct cctcaacgac tttataattt ggaatactag
     2161 ctgcctttga aaccatattc ttgctccatc tttattctag ctctctcttt ccattcagtc
     2221 gctcccgttc ctatcgttcc tgtaaccaga tacctcggtc gcatcatcct ttactagaac
     2281 caacagacgg tctcagcaat acccgtttct gtgtatttgc aggcagtgca gtagcccacg
     2341 ctcttccgat acaagtcatg catttaattt aaaacgagta ccgccattga gataccgact
     2401 caccagcgat gacagggcat ttttgagtta aaccactgcg atggctatta caaacagccg
     2461 gatgaattag cagcgcattt ttgacgcagc aatgctccag tgcgagaggc atactgggca
     2521 ggattcacgc cgccctgaca gggctgagct acttagctgc taactgggct ggtagtgtct
     2581 cagtttggtt ttgacaggct caaaataagc caccgggatt gtggcataca aggaaatcgt
     2641 tcttctggca aactagcgca caatggcaaa gacaatctat ttcgttcgca ttggaggcac
     2701 taatcagtgg cagcaatgca cgcatggcgt cccacaacgg ggcggtggtc tggcttattc
     2761 tataggtgac aggcaaggtt ccgtctgtga taaagactgg aaaacgaaac cctcttggcg
     2821 acgatttttc atcaagcaat ttgcgagatt tcaaatttga ttctaaattc aaactgagac
     2881 actaccggct tttcgattcc ttgtaaccct tcttgtttgt atctttggcg gcttgttcga
     2941 gcgaggcgcg ggtgaaaagt tccggggaca tgtcggagtt atatttacag ctggacaaaa
     3001 atgtttggga aactttgcga tcgttgcgag aaagctcaat gctcagcggt gtttttactc
     3061 cgtcgttcaa cgcatattgt gtgaggctgg tgatcacttc ggcgcgctcg cgagtttctg
     3121 gattgcgcgt gaccacaacc caacgcagcg gcagatgcga atatttatcg atgcccagac
     3181 gcatcatgcg gtgttcgcga tccgtaaact caatccactc cgcttccttt aagtcgatta
     3241 aatcggtgcc cgcgtactcc gcttccacgc ccggttcgtt gatccgcttg cgcagcatgt
     3301 tgttcatatc ggttttcaat tgatcgttaa aattctggat caggtcgtcg ggctgattgg
     3361 acactccgct tttatccagc gtccaacctt ccttgccgtt aaaaacggtg atcatcgcgc
     3421 cgccgtgcgt aatcaacagg tcatcggaac ccatcagaaa accgacaatc gtgtgttcgc
     3481 ccttggtgaa gtactcgacg cgatttttgt ccggcagcag ccaggtatcg cggaacagag
     3541 tgaagcccat gagggttcca gctgtcccga acgtggcgtc gcgcccgtcg cattgcacgt
     3601 tctgcacgtt caggtatgct ggcccaccca gagcggcgat gacctgcttt gtgatacgtc
     3661 gccccttcgc gacattcgtt tcaccgggtg cttcctgcgg ttggccagcc gacgcgacca
     3721 tttcgcacgc acacacgaca cccactaaca aaaataagca caccgttgct cggatcagcc
     3781 gcacaggaag gaacctccac ctacatggaa ggcattttgg accgcaaagg ttgccagatt
     3841 cagcaacgcc acgcatgaac taacgtattt taatgtcagg cagatgcccg ttcgtagagg
     3901 cgacgaatgg gctccgcgga aaacaattcg cagcgcgtcc gggtaagctg cgaagaaaag
     3961 cgctgcttac gcttcttttt cgatgaccgc tttgaattcc gccgtgacgc cgtgaacaag
     4021 cttcacggtc acaggcttct cgccgagcgt cttgatcggt tcatcgagtt gaatgcggcg
     4081 cttatcgatt tcaaaaccct gcgccttgag cgcatcggcc agatccgcgg aagtgacgga
     4141 accgaagagc tgatcggtct cgccggcctt gcgcgtaaac ttcacggtaa cctggttgag
     4201 cagagcggcc tgttgatgcg ccgcttcctt ttccgtggcg gtgagcttgg ccagcgtgac
     4261 tcggattttt tccagccgct tgatgttggc gggcgaagcc tccagcgcga gcttgtgcgg
     4321 cagcaaatag ttgcgcgcat atccggcggc gacttccacc acttcaccgc gtgttccgag
     4381 tttttcgacg tcttcttgca gaataatttg cattggcgtt cctcttctaa aagtctaaat
     4441 ccagggtcgt tcagtccctg ctatatccta ctgttgtgcc cgattctgtc ccgcctgctg
     4501 tagcccgcct cttcagaggc ggggtttaga tgacgcacga agaaaatcct cgcctctgaa
     4561 gaggcgagct acaaagtgct acaaacccgt cctacaactc tgtcgcgaag ggcagcagcg
     4621 cgatattacg cgcacgcttg atggcttcgc caagccagcg ctgatggcgg gagcaggtgc
     4681 ccgtcatgcg gcgtggcaga atcttgccgc gctcctgcac gaacggctga agctggtcgg
     4741 ccttcttgta atcgataaaa tccacgtgct cgacgcagaa gcggcatact ttcttcttgc
     4801 ggaaatactg gcgcttcccg cctcggcgtg ggccgcctgg accgccgccc ggccctccgg
     4861 atcctccagg accgccttca cggcgtcctc cgcctccccc cgaatgccct cctccggaat
     4921 ggcctccacc agagtgccct tcggaacggc gcggtggtcc gcctgtgtgt ccctcggcgc
     4981 gatgcgccgg ggcagaagcg ggagtcggag ttgcgctggg agcgtgtggc tcctgtggtg
     5041 ctgatggtac tggttggtca gacatcatgt ttcctttcgc cttagaaatc aattcgcacg
     5101 ccgcgtgtta ttcggcggcc ggtgcgggag cttgctgctg ttgcggttga ggagctgccg
     5161 gagcggcggc tgcctgcttg cgcgggcggc ggctggcgcg gcgctcgcga tgattgccga
     5221 gcttttgctg gcgcttcagt tcttcgtcca gacggatggt ctggtacttg attacggcgt
     5281 cagaaacttt cagacgtcgt tccagttctt tgatcaggtc gccctgggtg ctcttcacga
     5341 ccatatatac gaagaagcct tcgcggtgct ttgccacgcg ataggcaagg cgtcgtgtgc
     5401 cccagcgttc cgttttttcg atctttccgc catgctcgcc ggtggaatgt tcgagggtgg
     5461 tcacaatctt gttgatctct tcttccggcg tggccggcct gcagatgaag atcaggtcgt
     5521 atattcgttc ttccatgttt tgttccatat ttttggtact ccgtttcgca aactttaaat
     5581 tgctcgtgcc atttcgcggc gcgttgcttc cttccgcgga aatgccccgc ctctgatccc
     5641 tcgttttcac tcaggacaag cgagacggct acagtaaaac cgaaaactgc ttaactttcg
     5701 ttttgctcgc cgttattctc gtccggcgat acgcggcgat tgaagcggtt catcgctgct
     5761 tgcgttcctt ccgccaaaat tgctttcacg gcttcggcgc cttgctcaat ttcttccgcc
     5821 accagatctt cctcatcgcg ccgaatctgg cgaagcacgt actctctcag atcgccgcgc
     5881 atttcttccg gctttacgcc gagccgcacg cgaacaaatt ccacggttcc cagcgcgcca
     5941 atcacggatt tcagtccgtt atggccgccg gccgttccgc gctccctgac gcgtagcatc
     6001 ccccaaggaa gcgcaatatc gtcgcaaaga acgatcacat cggacggctc gcactctgcg
     6061 cggcccacca gttcccgcac cgcgaggccg ctcaaattca tcatcgtctg cggtttggcc
     6121 agcactacat cttgtcccgc aatctcgccc aggcctacca gcgatttcga ttcggggcgg
     6181 ctcacccgga tgccggcgtc ttcggccagc gcatcgacca ccagaaaacc caggttgtgc
     6241 ggcgtccagg catattcgat gcccggattg cccaagccaa caattacgcg catctgcggc
     6301 tccggtagat tgtctcgcgg cactgcgtcc gcgaaagcgc gcgatgccaa cgcagaatgc
     6361 cagggcgtag caaacgcgtg ttacttcttc ttttccttct ccgcaggcgc ggccgcttcg
     6421 ccttcttcgc cctcttcgtc cttcttgcct ttcttgataa gttcgggctc ggctggtgct
     6481 gcggcggctt ctccggcaac tgcttcgggc gccttttctt cctcgacgcg cagggcgaca
     6541 acgtgtgcga gcacgcgcag gggatccgtc attaatttca ttttttgcgg atccagcgga
     6601 atgtccgacg cgtgaatctg catgcccatc atcaaattcg aaatatcaac tttgaattcg
     6661 gtcggaatgt cgactggcag gcactcgatt tcaatttcgc gggtcacaac ttcgaacacg
     6721 ccgccctgca gcttaacgcc ctgtggctcg ccaaacgtgt gcaccggaac cttgatgcgc
     6781 atacgcacgt ccatggcgac gcgcaagagg tccacgtgaa gcagcgtgcc cttgatcgga
     6841 tcgacctgat attccttcac gatcgcgaaa tcttctccgc cctcggtctt cacggttaga
     6901 atcgtgttgt gtcccgtcgc ggaacgcaga atcgctgaaa cgtgttttgt attgacctga
     6961 agcgcctgcg gaccgccctt gccgccgtaa agaacggcgg gaactgttcc ggcaacgcgg
     7021 aggcgccgcg ccgcattctt gcctcgcgat tctcgtggag ctgcttctac gataatctgt
     7081 tccataaatc tttccccatt tcatggccaa aatttcctcg gagttcagga aatcatttgg
     7141 cgttgttgaa tgcttcaatc caaaatcatt tgcgctagta aaacagttcg ctgatcgaag
     7201 tctcttcgtg aatcgaatga atgccgcgcg ccagcaagtc agcgacgctc aggactttga
     7261 tctttgaaac ttttttcgct tcttctgaca acggaacgga atccgtgacg atcacttcct
     7321 caatttgcga tgcgcagatg cgctgcacag ccggtccgga aagcactgga tgcgtgcagg
     7381 ccgcgtaaac tttcgtcgcg ccttccttca aaagtgcctc tgccgttttt accagcgtgc
     7441 ccgccgtatc aatgatgtcg tcaatgatca gcgtggaacg cccgcgcacg tctccgatca
     7501 ggtgcatcac ctcgctcacg tcaatatcca cgcggcgttt atcgacgatg gccagtggtg
     7561 aatcgagctt cttggcgaag gcgcgcgcac gttccacgcc gcccgcatcg ggtgaaacaa
     7621 ccgtcagatt tcccattttc ttttgcataa aatgctcgat cagcacgggc gaagcgtaca
     7681 ggtgatccac cggcaccgtg aaataaccct gaatttgcgg cgcatgcaaa tcaagcgtca
     7741 aggcgcgatt cgcgcccgcg gtttccatca ggtcggcaac gagcttcgcg gagattggca
     7801 ctcgcggctt gtccttgcga tcctgtcgcg cgtagcagta gtaggggacg acagccgtaa
     7861 ttcgccaggc cgatgcgcgc ttgaacgcgt caatcatcat caacagctcc atcagatgga
     7921 aatccgatgg attcgagcac ggctgcacga cgaagacgtc cgcgccacgc acattttcca
     7981 gaatttgaaa gtacacttcg ccgtcgctga acttgccgag ctttgcgtca cccagcttca
     8041 cgttcaagtg ccggcaaata tccgccgcca agcgcggatt cgcgctaccg cagaaaattt
     8101 taaatttatc gtcggtcaag tttgttgatc cttgcgagtg ctcgaactat ttggacaaga
     8161 tcgcgcccat cttttggcga tactccaccc tcgaaagagt tgagcagaga aaaacctgat
     8221 cctgcggaaa cgctcgggca gctcggcgcg ccatcgctgg gtgttgatag attccaaaca
     8281 ccgccgaacc acttccggcc agcgaggcct ctgcggctcc ttgttgaagc aactccctct
     8341 tgattgcatc caagcgggga tacctgggga aaacagcagc ctcaaaatcg tttgagagcg
     8401 tgcccccctg agagctccaa cacagagcac agaactgaag tagtctagat gggtcttccc
     8461 ggtttgtcaa ttcctctgac tgcgcctcgg agatccaccg gtaggcatct tttgtgggca
     8521 cggcaatttc atgcggcgaa accatgagca gggtgcgctt tggcacatca ggcagaggat
     8581 aaatttcttc accgcgcccg atgcccagcg cgcgacctcc ctgcaaaaaa aacggaacgt
     8641 ccgacccgag gccggacgca atggtcataa ggcgctcaag cggaatacgt tttcccgcaa
     8701 ggcgcaaaag ccccacaagc gcggcggccg catcactcga cccgccgccc atcccgcgtc
     8761 ctacgggaat gcgtttggtc aataatgcgc gaactccctg cttcagtccc aactcacggc
     8821 tgagcacatc caacgcacga tagacaagat tgttttttcc cggctcccca gcgagttgcg
     8881 aatttccgtc aacgcacaaa tccacgccag gttcgcgctt ggattccaga actaaagtgt
     8941 cgtgcaacga aatggtttgg aaaatagttc gcagttcgtg atagccatcc gcgcggcgcc
     9001 caaggacagc aaggcgcaag ttgaccttcg cgtaggcggg tatacgcacc gatttcataa
     9061 gggggatatg ctactacggc ttcaggttgc cgggctgttt ctgctgggca agcctgtgct
     9121 taagcgcgga aattttttgc tcaacttcag ccatttttgc gggctcgaat tccgcgggaa
     9181 gagcgcggtg ccattcatcg acggatttct gccactgggc ttcggccaga ctttctttac
     9241 ccatcttcgc gtagatgtct cccaaatggc tcaaaatggt tgggtcgtga ggctcgtgtt
     9301 ccagggcctg gcgcaacatg tcttccgcct ctgaaagctt atcttgcttg taataagccc
     9361 atcccaggct gtctttgtaa gccgcgctgc ccggctcttc gacaagcgcc cgtttaatta
     9421 gagaagtggc ttcgtccagg cgcacgccac ggtcggcaag catgtagccg taatagttga
     9481 gcgcggagga attgcgcgga ttcagctcca gcactttgcg gaattccgtt tccgcctgat
     9541 cgaatttttt ctggcgctca aaaatcgcgc ccatcaaaaa cccggtcatt tccttgcttt
     9601 gtgaatctcc ctgcagtttt tccgccgcgt gaatcgcggt ctccgcatcg ttccagcgct
     9661 tgctctcttg gtagacctgc gcaatatcca gttgcacttc gaaatcgctg ggagaaccgt
     9721 ccagcaggcc gcggagctgc gacacggctt cgtcggcttg cgcgttttcc cctagcagca
     9781 aggcctgcgc gatgcggatg gaatgatctt tgggataggc ttcgaccgct ttatgcgcgg
     9841 catccaacgc tttggaaatg tcgcgcgcgt cgcggtacgt attcacaatc aaaatgcgcg
     9901 cgcggcggtc ttcctcggtt ccgaggcgca gcatttcttc aaacgtgttt accgccgccg
     9961 agtagttgct cgattcccga tacaactgcc cgagttgctg gtacaggatg gcgagcgtgc
    10021 gccggcgcga tggagcgaat tccgattctg tcttgaccgc ggttacggct tccgaaagca
    10081 ctcgaatcga atcgtcaatc cgcccttcat cctcataaat cgtggcttcg taatagatga
    10141 cttccaggtt attgggcgtg cgctgcttcg cgagcaatat gttttgctcg gctttatcca
    10201 gctgcttcat ctggcggtaa atttcggcca accgcaaata attgtcgccg tcgtccgcat
    10261 ccatttccac caggccctga tattgttcga gggcctcagg aaatttttct tcggccagaa
    10321 gcgtctgcgc cagttcttta tggtgattca gatcgcctgg ctgagattcc gaggctttcc
    10381 ggtacgcctg ctcggcattc ggaagatcgt gaatctggtt gtaagcgtcg cccagaagtt
    10441 cccatagccg cggcgtgggc gctcgctgca gaatgacttg cagggaggag acagcttcct
    10501 ccgattttcc ttcatccagc agcagttgag tgagttgctc gacgccattc tcgttcgcgg
    10561 gttcgcgtgc aagcagggcg cgcagcacga cttccgcttt ttcctgttcg ttttgcaggc
    10621 ggtagagccg tgcgagccag agttccgcat cgctgtcatt tggatcgagg cgaacaattt
    10681 cgcggtattg ctccacggcg cgggtgatcg tgtcgcgctg tcccgatgcg ttgctcaggt
    10741 cgccaagcgt gcgcacgtaa attcgcgcca gcaagcgtcg tgcaggcaga ttgtccgggt
    10801 ccttggccag aatcgaattt gcttccgtga ccgcgtcgcg aattctctgc gactggtaat
    10861 aaatttcggc cagttcgtcg ccaatctgct ggctgttggg gtcgagcgtg aacgctctct
    10921 taaggaaatc gatggccttg tttgcgtctt ctgcatggct ggaactttga aattcctgcg
    10981 agtagtaatg ccccattaca aaattatcgt aggcatcggc ttgcgcggcg attcccgagt
    11041 cgaggtctga aaccggggcg gaagtttgcg cgctcggcac tcttttacct tgctgcgcgc
    11101 acaaagcaga tccgcccgcg agcgagaaga taaatagtac agcgagatgt cgagcaattt
    11161 tcatctatat ataccgcata ccgcagaaag agattatccg ccacacggcg ttacgcgtca
    11221 aaactcatcc gcaacgaaag ttccttctcg taatcatctc cctgtcctcc ctggccgcaa
    11281 aaaagcaaat ttagtgcctg aaatgccgca ctccgcagaa aaccatcgcc attcccagtt
    11341 gatcggcggc agcgatcacg tcaccatcac gaactgagcc gccgggctga atcacggcgg
    11401 ttgcaccggc ttttcccgct tcttccaggc cgtctggaaa aggaaaaaat gcgtcggagg
    11461 ccactacgct tccggccaac ggcaactgcg ctttcatcac tgcaattttc acggagtcca
    11521 cgcggctcat ttgtcccgcg cccacgccca cggtttgccc agcgcgcgca aaaacaatcg
    11581 cgttggattt cacgtgcttg caaactttcc agccaaacag cagcgcctca atttcttcac
    11641 gtgtgggcgc gcggcgagta accactttta gatgcgtctc ggcaagttcg tgagcgtcct
    11701 gctcctgcac caggatgccg cccgaaatac gttttaattc aaggccgaga tcgcgcgaag
    11761 agccttcgcg ggcaggcagg tgcaataatc gcagattttt cttggtcgca aatttttcaa
    11821 gcgctgccgg ctcatagccc ggagccacga tgcactcgac aaaaagtttc gccacttcct
    11881 cgccggttgc ggcgtcgacc acgcgattaa acgccatcac gccgccaaac gcggagatcg
    11941 gatcgcaggc gagcgctttc acgtaagcat ccacaagcgt ttcttgttcc gccgtgccgc
    12001 aaggattgtt gtgcttcaca atcacggctg ccggccgctg gaactccatg gccagattga
    12061 gcgccgcttc gaggtccaca aaattattgt aggaaagctc tttgccctgc aactgccgtg
    12121 cagctgcaag cccgcttgca tggattcccg caggcacgta aagtgcggcg cgctggtgcg
    12181 gattttcgcc gtagcgcaat tcctgccgcc gcgcatatgc aaaatgcagc cgctcaggaa
    12241 gcagtgggcg ctcggcgagc tcaatcgagc cagtgctcgc ggacagacgt tccaactccg
    12301 tggcaatttt gccgtcgtag cgcgcggtgg tcgcaaatgc tttgcgcgcc aattcaagcc
    12361 gcgtcgcgag actcacttct ccatgcgctt tgatttcatc ggcgattgcg gcgtagtcag
    12421 ccgggtcggt gaccaccgcg acgctttcaa aatttttcgc cgcggagcga accatcgtcg
    12481 gcccgccaat atcgatgttc tcgatcagat cttcggaagt caggtccggc ttcgccgcag
    12541 tcgcctcaaa gggatacaga ttcacgatca ccagatcgat gggtgcaatt ttatgttccg
    12601 ccgtctgctg cagatcgccc gcttctttcc ggcgaaataa aatgccgcca tgtactttcg
    12661 gatgcagcgt tttaacgcgc ccgcccagca tttccggcca ccccgtcagc tcggaaacgt
    12721 cgcgcacagt aatcccatcg tcgcgcagca gcttggccgt gccgccagtc gatacaatct
    12781 caatcccaca cgcaaccaac tgccgcacaa attccgtcaa gccacttttg tcaaatacac
    12841 ttactaacgc gcgctgcaca gccacagaag tcctccaaag ataaaagcaa aacgcctatt
    12901 gtatgcgcgc gcgcgaaaat cgaaaagcgg gaactaagat tgaccggaag gaaacaatgc
    12961 agcttatagt cggtttttcc aatacccagg ttttctgtgt ccatgcgcta ctgccaaaac
    13021 ctggacccct gagggaagtt ctcgaaagta gattgcaaat ggaaaccgtt cgagcacgaa
    13081 tcttcgggta tcgcgggtcg acttccgcca acgttttggc ccgtcggaga tcattgcaat
    13141 ggcatgactc acttcctcga cgaacttgga agccacaaat tcactgcgga gatagtacca
    13201 atcaaatgca gcctcgaact ccgcgctcgc agcttcgtga aaattaactt caactgccat
    13261 gggcgagtct gtcggaaatc ctttgccgga gctcttccca agaaactgtc ttcgccttgc
    13321 ctgagtctag ttcagcgatg cgcctcccaa tttcctcgtc ccatgcgagt tcagcccctt
    13381 ggtcggggac ttcttccaag ctttcgataa gtgagcgaac aagtgcagtg cgctcctgct
    13441 ctggtagtgt aagcgccttt tgcagtaatt cgagagcctg ttgcgtcata ccgttattgt
    13501 accttctgtc ggaacccctt gcacgtccct gtgccttctc cgtttccgaa ttcttccgat
    13561 tgtcaaatcc acccggcaat gcggttctag ctccgtttga agaattgaat ttgacaaagg
    13621 aaagaaaacg tttctttcgc gccagaaaac attggcctga tggaatcgaa tagccagttt
    13681 gcaaccgatt gcaaatcttc gctccctgcc tcgaccgcgg aaattacatt ggtctgggta
    13741 accagatagt tgacgagttg ctcgcttgaa aattcaattt catgtgtgaa ttcctcaaaa
    13801 ccatatcgcg cgatcccata ttcggatgca ttcatctcag ccaatggctg aacgttgcga
    13861 ggcggagttg gataccgcgc cagatattcc tcgtgatacc atttctcgca gtcatcgttg
    13921 cctttcattt ggcctgggaa tacgtcgttg taaatgatta gccaaccttc gggccgcaac
    13981 aaccggtgtg cttcgagaag aaactttcgc tggtcaaacc agtgaaatgc aagccctacg
    14041 gtcatcagtc caaattccgc attggcaaac ggcatctgtt ccgcctgacc ttcaacaaaa
    14101 cgaattcgtt tgtgttgaat tgcatgagaa agcatttgg
//
LOCUS       DASZMB010000245         3610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1881313, whole genome shotgun sequence.
ACCESSION   DASZMB010000245 DASZMB010000000
VERSION     DASZMB010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1881313"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..965
                     /locus_tag="VG272_11510"
     CDS             <1..965
                     /locus_tag="VG272_11510"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWF14356.1"
                     /translation="KRLPQDGRIMIKYRKEGKIKDLDFRVSTIPALYGEKIVMRLLDK
                     ENLRLDMTKLGFEPESLAKFERAILRPYGMVLVTGPTGSGKTNTLYSSIAKLNTPETN
                     IMTAEDPVEFQLAGINQVQMKDQIGLNFAAALRAFLRQDPNIILVGEIRDFETAEIAV
                     KAALTGHLVLSTLHTNDAPSTISRLMNMGIEPFLVATSVNLICAQRLVRRICANCKEI
                     LNLPPQAMLDAGYTEEELPKTQIFIGKGCGTCNKTGYKGRVGLYEVMEISDELRELIL
                     VGASALELKKKAVDNGMLTLRRSGLTKVALGQTTIEEVVRETVL"
     gene            993..2108
                     /locus_tag="VG272_11515"
     CDS             993..2108
                     /locus_tag="VG272_11515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HWF14357.1"
                     /translation="MPGITLSELLKKMIELGGSDLHITTNSAPRVRVHGKLKPMDMAP
                     LTAADTKALAYSVLTDAQKHRFEENLELDFSFGLKNLARFRGNLFQQRGAVGAVFRTI
                     PWEIKNFDALGLPAVVKGLCDKPRGLVLVTGPTGSGKSTTLAAMLDKINSERDEHMLT
                     IEDPIEFLHNHKRCLVNQREVNSDTHSFAGSLRAALREDPDVVLIGEMRDLETIESAL
                     RIAETGHLTFATLHTNSALSTINRIVDVFPSFQQPQIRAQLSMVLEGVLCQSLLPKVD
                     GRGRAMIMEVLIPNPAIRNLIREDKIHQIYSAMQTGTGTTGMQTFNQGLANAYFQKLI
                     TLDVALSRSSNPDELQDMINRGVSSPTSRTPAAPVRR"
     gene            2244..3458
                     /locus_tag="VG272_11520"
     CDS             2244..3458
                     /locus_tag="VG272_11520"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWF14358.1"
                     /translation="MPVFTYRGTNRAGATVSGESAATSKSELVTSLKRQQINVTKMSE
                     KGKEFSLPTFGGGVDSKELAVFTRQFSVMIDAGLPLVQCLEILAGQQENKVFQNVLTG
                     TRAAVESGSTLSAAMRQYEKVFDALYVNLVEAGETGGILDTILQRLSTYIEKNVKLKR
                     AVKSALVYPIAVLSVAAGVIILLLWKVVPIFATLFLGLGVDLPLPTRIVIGLSNFVGS
                     IFGFLILVALAGAIVGLKVWYKTPVGRMALDRVLLKLPMIGLLLRKIAVARFTRTLGT
                     LISSGVPILEGLEITARTAGNAVIEAALMRVRKALEGGRNLADPLRETDVFPGMVTQM
                     IGVGEQTGAMDAMLQKVADFYEDEVDAAVKDLLAALEPAMIVVLGLVVGGIVISMYLP
                     LFSLIGQLSKAH"
BASE COUNT          842 a          996 c         1003 g          769 t
ORIGIN      
        1 aaaagcgcct gccgcaagac ggccgcatca tgatcaagta ccgcaaggaa ggcaaaatta
       61 aggatcttga cttccgtgtt tccacgattc cggccttgta cggcgaaaaa atcgtgatgc
      121 gcttgttgga caaagaaaac ctgcgcctgg atatgaccaa acttggattt gagcccgaat
      181 ccttggccaa attcgagcgg gccattcttc gtccgtacgg gatggtgctg gtcacgggcc
      241 ccacgggttc cggcaaaacg aatactttgt attcatccat cgcgaagctg aacacgcctg
      301 aaacgaacat tatgaccgcg gaagatcccg tcgagtttca attggcagga atcaaccagg
      361 ttcagatgaa ggatcagatc ggcctgaatt tcgcagcggc cttgcgcgct ttcttgcgcc
      421 aggaccccaa cattattctg gtcggagaaa tccgcgactt tgaaacggcc gaaatcgcag
      481 tgaaggccgc actcaccgga cacctggtgc tctccacgct gcacacaaac gacgcgcctt
      541 ccaccatcag ccgcttgatg aacatgggta tcgagccgtt cctggtagcc acgtcggtga
      601 acctgatttg cgctcagcgc ctcgtgcgca ggatttgcgc caattgcaaa gaaattctaa
      661 accttccgcc ccaggccatg ctcgacgccg ggtacaccga agaagaactt cccaagacgc
      721 aaatctttat cggcaaaggc tgcggcacct gcaataaaac tggatacaaa ggccgcgtag
      781 gtctctacga ggttatggaa atcagcgacg agctgcgcga attgatcctg gtcggcgctt
      841 ctgctctcga attaaagaaa aaggcagtag ataacgggat gctgacgctg cggcgcagcg
      901 gcctgaccaa ggtcgcgctc ggacaaacca cgatcgaaga agttgttcgc gaaacggttc
      961 tttagaaacg gctctattag aaaggtaaaa cgatgcctgg aattacatta agtgaattac
     1021 tgaaaaaaat gattgaactg ggtggcagcg atctgcacat caccacgaat agcgcaccgc
     1081 gcgtgcgcgt tcacggaaag ctcaagccga tggatatggc tcctctaacg gcagctgaca
     1141 ccaaggcgct tgcgtacagc gtgctgaccg atgcccaaaa acaccgtttt gaagagaatt
     1201 tggaactcga cttttccttt ggattaaaaa atctggctcg cttccgcggc aacctctttc
     1261 agcaaagagg cgcggtgggc gccgtctttc gtacgattcc atgggaaatc aagaattttg
     1321 acgcgctggg cctgcccgcc gtcgtgaagg gcttatgcga caagccgcgc ggccttgtgc
     1381 tggtcacggg cccgacgggt tccggcaaat cgaccaccct cgccgccatg ctggacaaga
     1441 tcaacagcga acgcgacgag cacatgctga cgatcgagga tccgattgaa tttctgcaca
     1501 atcacaagcg ctgcctcgtg aaccagcgcg aagtgaattc ggatacacat tccttcgctg
     1561 gttcgctgcg cgccgccttg cgcgaagatc ccgacgtagt actgatcgga gaaatgcgcg
     1621 acctcgaaac gattgaatcc gcgttgcgca tcgcggaaac cggtcacttg acgttcgcca
     1681 ccctgcacac gaactccgcg ttatcgacca tcaaccgtat cgtggacgtt tttccgtcgt
     1741 tccagcagcc gcaaattcgt gcgcaactct ccatggtgct ggaaggagtt ctttgccagt
     1801 cgctgctgcc caaggtggac ggccgcggcc gcgccatgat catggaagta ctgattccca
     1861 atcccgcgat tcgcaacctg attcgcgaag acaagattca tcagatttat tccgccatgc
     1921 agaccggaac cggcaccacc ggtatgcaga cctttaatca gggtttggcc aatgcgtatt
     1981 ttcagaagtt gattacgttg gacgtcgcgt tgtctcgttc gtcgaacccg gatgaactgc
     2041 aggacatgat taaccggggc gtgagttcgc ccacgtcgcg tacgcctgcc gctccggttc
     2101 ggcgctgata ccgggacgcg ttgcatttgc tatacgccgg atttaaatgc gggtcggaat
     2161 gtggcgcccg caaagattag cgacaccatc cccgaagcac cagttcgatg tcgggttgga
     2221 tgacttttga ggaggaacga acgatgccgg tttttactta tcgaggtacc aatcgcgccg
     2281 gagccacggt cagcggtgag tcggcggcca cgagcaagtc ggaactagtc acaagcctta
     2341 agcgccagca gattaacgtc accaaaatgt ccgagaaggg caaggaattc agcttgccga
     2401 catttggcgg cggagtcgat tccaaagagt tggccgtatt cacgcgtcaa ttctccgtga
     2461 tgatcgatgc tggtttgccc cttgttcagt gcctggaaat tttggccggg caacaggaaa
     2521 acaaggtttt tcaaaacgtt ctgacgggta cgcgcgcggc cgtcgaaagc ggctcgacac
     2581 tgtctgcagc catgcggcag tacgaaaaag ttttcgacgc gctgtacgtc aacctagtag
     2641 aggccggaga gacaggcggt attctcgaca ccattctcca gcgcctctct acctacatcg
     2701 aaaaaaacgt gaagctgaaa cgcgccgtga agtccgcgct ggtgtatccc atcgcggtgc
     2761 tatcggtggc ggccggcgtc atcatcctgt tgctgtggaa ggtcgttccc attttcgcca
     2821 cgttgttcct gggattgggc gtggatcttc ccctgccaac gcgcatcgtt atcggcctca
     2881 gcaatttcgt cggcagcatt ttcggattcc tgatcctggt ggccctcgcc ggcgcgatcg
     2941 ttggattgaa ggtttggtac aagacgccgg tcgggcgcat ggcattagac cgcgtcctcc
     3001 tcaaattgcc gatgattggt ttgctgctca gaaaaatcgc ggtggcacgc tttacgcgca
     3061 cgctgggcac tttgatttcc agcggcgttc ccatcctgga aggcctggaa attacggcgc
     3121 gcaccgccgg caacgccgtg atcgaagccg cattaatgcg agtgcgtaag gcgctcgaag
     3181 gcggccgcaa cttggctgat ccgctgcggg aaacggatgt cttccccggc atggtcaccc
     3241 agatgatcgg cgttggcgaa cagacaggcg ccatggatgc catgctccaa aaagtcgcgg
     3301 atttttacga agatgaagta gatgctgccg tgaaagattt gttggcggca ttagagccgg
     3361 caatgatcgt cgtccttgga ctcgtggtcg gcggcatcgt tatttcgatg tacttgccgc
     3421 tgttctcgct catcgggcag ttgagcaaag cgcactagga gccgtaaggg cggactccaa
     3481 gtcggcaagg atcgcgagag cagcccatga ggctgctctc caccactttc agacccagac
     3541 ctgatcgaaa attttcagaa agcgaaagga agcttggaaa caagcacgcc ctgaatggaa
     3601 ctgacacaaa
//
LOCUS       DASZMB010000246        10641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2165236, whole genome shotgun sequence.
ACCESSION   DASZMB010000246 DASZMB010000000
VERSION     DASZMB010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 10641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10641
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2165236"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VG272_11525"
     CDS             <1..408
                     /locus_tag="VG272_11525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14359.1"
                     /translation="IQDPWPVRERIAVCFSSNPRAQYLIARASRMARRMDAELYAIYV
                     DIGVDTSEQDKNSLNANIQFSESLGAKVVKLIGKDVSSATAEFVREKHVTQVIFGRSA
                     VEGWRKYLYMTAIHRFLRDAPAVDVHIVTQEPD"
     gene            711..1835
                     /locus_tag="VG272_11530"
     CDS             711..1835
                     /locus_tag="VG272_11530"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HWF14360.1"
                     /translation="MAHLFDPLPIRGVTLPNRIGVSPMCQYSSDDGFANDWHLVHLGS
                     RAVGGAGLVFVEATAVTAEGRITSEDMGIWKDAHIDFLARIVRFMKTQGTVPAMQIAH
                     AGRKGSTQRPWVGNSKVAIEDGGWVPVAPSAISFSETYPMPRALEINEIHSIVDAFAA
                     AARRALQAGFQVLEIHAAHGYLAHEFFSPLSNFRTDEYGGSFENRTRIGRDIVTAVRK
                     VWPENLPLFIRISAQDWKEGGWDLDQSVDLAKQLKKLGVDLVDCSSAGLVVDQKIAAG
                     PCFQVPFAERIRRDANILTASVGLIETKEQVADILARNQADLVLMAREFLRDPYWPLR
                     TARELKQPLTWPAQYLRAAPADSKARMPTADAASKVNVLD"
     gene            1971..3587
                     /gene="murJ"
                     /locus_tag="VG272_11535"
     CDS             1971..3587
                     /gene="murJ"
                     /locus_tag="VG272_11535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HWF14361.1"
                     /translation="MSKHSVHRRVFTSTGIVMAGILASRLLGFFREWAVAHQVGSNAM
                     TDAYYAAFTLPDFLNYLVAGASLSATFIPVFAKYSAEGNEDEAWWVFSTVMTFMGGLL
                     AALILAGEIFAPQLANLIAPGFAPPERQRLIFLTRLMLPAQFCFYQGSILSAVQYAKG
                     RFVIPSLAPVVYNVMIVAGGILLASRYGMTGFAIGVLVGALLGNFLLQVYGAHLAGAH
                     FRPNLNLRHPGFILFLRLSVPIMLALSLVFTDDWIIRWFGSYLQPASITWLSYGKTLM
                     RVPLGVVGQGVGVASFPFLAQLYSEGKIDELNRLLNTTMKWMIVLLVPISALTIAESS
                     PVVYLVFSHTRLRGPDFNATAATLMLFSLGMFAWGAQNILARGFYATRNTLTPAIVGT
                     ILAIANIPVYWVLVKHFQHIGLALASSLGITAYTVVLFVLLNRRTKNSDAASITLFFL
                     KVCLASTVVAYICHRLQRLLEPHFAWHTIPGAFALLMMVTSAGIVLLFIAAKLLGIRE
                     FDELGARARKLLSRNAPEAVVPVASNHIDG"
     gene            complement(3649..3999)
                     /locus_tag="VG272_11540"
     CDS             complement(3649..3999)
                     /locus_tag="VG272_11540"
                     /inference="COORDINATES: protein motif:HMM:NF042601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility-associated protein"
                     /protein_id="HWF14362.1"
                     /translation="MFSIIGIVIVFGCVAAGYLMEHGNLRVLMQPAELIIILGAAIGT
                     VLIANPLHILKKIGGGFAKAFGGSPFNKQRYTDDAIEKHGVRGSTVRVLQKPFTHDTL
                     ARGVRDALGPPRQP"
     gene            complement(4033..4944)
                     /locus_tag="VG272_11545"
     CDS             complement(4033..4944)
                     /locus_tag="VG272_11545"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HWF14363.1"
                     /translation="MPELNDPAIYQTVLDALDTGVYIVDRNRRIRFWNEAAEKITGYL
                     RQDVVGHFLRDHLLTTSDGAGDLDLDPLDPINIAFRDGKSSLLSVSILHKEGYRLPIT
                     LRTNPIRNSHGAVIAAAESFDKTIPFLDSTRRHSGLADFGCLDVLTGIAAQGFMDTRI
                     RENLITFGEHHIPFGILLLQIDELDQHRTKRGPGVVPTILRIIAQSIENCLRPTDVLG
                     CWGKYQFLAILTDCKEMEVPLVGERARRIISRSKIEWWGDKFSVTSPVGGAGCRTGDN
                     VESLIARADASLLESIAKGGNRVTVLP"
     gene            complement(4989..5714)
                     /locus_tag="VG272_11550"
     CDS             complement(4989..5714)
                     /locus_tag="VG272_11550"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWF14364.1"
                     /translation="MEKQQRNILAVDVGGTHIKVLAMGQKEPIKIPSGPKMTAKRMVK
                     EVREATKDWNYTVVSIGYPGPVIHGHPLSEPHNLASGWVGFDFRKAFGKPVRIVNDAA
                     MQALGIYHGGRMLFLGLGTGLGSTMIVDGVVEAMEIAHLPYKKGRTYEDYLGLRGLKR
                     LGKKKWRRYVAKIAEDLKNALEADYVVLGGGNAKLLKELPPGCRLGNNATAFTGGYRL
                     WDEPNEKSKWNKEAIPEKASKSN"
     gene            complement(5779..7596)
                     /locus_tag="VG272_11555"
     CDS             complement(5779..7596)
                     /locus_tag="VG272_11555"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HWF14365.1"
                     /translation="MPPIIAAQGLSKRYGAEPLFQDISFTVSEGERIGLIGPNGSGKS
                     TLLSILQGRVVPDTGDVAIRKRVRLCSVAQNSEFAEGATIRSVIANALDQAEVPEKDR
                     AARIAEPVGRAGFTDMDALTSSLSGGWRKRLAIVEGLVQSPDVLLLDEPTNHLDLAGI
                     QWLETLLQNATFACVVVSHDRYFLENIATAVVELARSYENGYLRVNGNYSTFLEAKEV
                     YLHAQRKRQEALENLVHTEIEWLRRGPKARTTKSKARIDTAHELIGELSDLNSRTKTS
                     SAKIDFSATDRKTKRLITLDGVTYDIGGRTLFAGIQFTVTSGMRVGLVGQNGSGKTTL
                     LRLLRGDIETTRGEVRRADALRIVYFDQNRELDPEISLRRALAPDSDSVIYQDRVIHV
                     AAWAAKFLFDPEHLSQPVGKLSGGERARVLIAQLMLQAADVLLLDEPTNDLDIPTLEI
                     LEESLLEYRGALVLVTHDRYMLDRVSTIVLGLDGLGGAERFADYSQWEIWQRAQQKGT
                     TNLSTGTKRATVQGEAQSAISKKKLSYHEVRELETIEERIAEADKNLEAKRVVLEDPA
                     VTSDPARLKTACIEMDEAQNILDRLYERWGQLEKKKVGG"
     gene            complement(7737..7880)
                     /locus_tag="VG272_11560"
     CDS             complement(7737..7880)
                     /locus_tag="VG272_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14366.1"
                     /translation="MQRKIFWTLFSGLGIIADLALPFWWALGATIPLFCVSWWVAYRS
                     DWF"
     gene            complement(8119..8937)
                     /locus_tag="VG272_11565"
     CDS             complement(8119..8937)
                     /locus_tag="VG272_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14367.1"
                     /translation="MRIRLEIGMLAVIVALSFSSSLSAQSSANPAATKPQKEGLMAGG
                     IPDLSGLWEPDFRGPESVRINTWDSSDLFAKHPEKAPMTPWAAAKFKDARPPFGARQT
                     FDNTNDPVQRFCDPPGIPRIYMYPWEITFIQTPKVVYILYEYTKVWRAVAMNTEHPKD
                     PDITWMGDAVGKYEGDTFVVDTIGLSDKTWLDQAGHPHSDALHVIERFRRLSQEKLEL
                     TVTIDDPKAYTKTFSASKLYNLSPGPMGETLCAYSEMKEFQENVVDKTQTPPGK"
     gene            complement(8986..9384)
                     /locus_tag="VG272_11570"
     CDS             complement(8986..9384)
                     /locus_tag="VG272_11570"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HWF14368.1"
                     /translation="MNWKLLIILVAIASLGMFSGAALAHHGVAGYDMTKTVTLHGTVA
                     KFDWSNPHVVVYVDAKNDSGESQHWTIEFAAPIHMVRAGWTKNAMKAGDDITIDTHPS
                     RNGAPVGISSTITFILKTVVNGGALPSHVE"
     gene            9786..10505
                     /locus_tag="VG272_11575"
     CDS             9786..10505
                     /locus_tag="VG272_11575"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HWF14369.1"
                     /translation="MDREPSCAGRDSVDSAERSSLAGYAGEIPASFDVLAAAARLGGA
                     GRLVENLAVVSERVERTPAVEVERIVSGREFCSGEKGGCGVGKTKRGKGTKWMVVVDG
                     RGLPLGNYLHSASPAEVKLAETTLAAIRVARSHHAGCPRQKPVRVIADKAYDSDPLRK
                     RLQKRGIELICPHKRNRVRPATQDGRALRRYRRRWIVERTNAWIGNFRRLVVRYDRSL
                     TIYGAFFHIACFMIVLRRVVQ"
BASE COUNT         2334 a         2997 c         2803 g         2507 t
ORIGIN      
        1 attcaagatc cgtggccagt gcgggaacgt atcgcggtgt gcttcagctc gaatccgcgc
       61 gcgcaatact tgatcgctcg agcatcgcgc atggcgcgcc gcatggacgc tgagctttat
      121 gcgatctacg tggacatcgg ggtcgacaca tcggagcagg ataaaaattc gctcaacgca
      181 aacattcaat tttcggaaag cctcggcgca aaagtcgtga agttgatagg caaggacgtt
      241 tcatcagcca cagcggaatt cgttcgcgaa aaacatgtca cgcaggttat cttcggccgc
      301 tcagcagtag aaggctggag aaaatattta tacatgaccg ccatccaccg cttcctgcga
      361 gacgcccccg ccgtagatgt tcacatcgtc acgcaagaac cagactgatg cttttaagtg
      421 gcgcgggctt cagcctgcgt gggttagcct tagcaaggac aaaattccac aggctgaagc
      481 ctatgccacg taaggccaca ccgaagttat attttaaact cttccaaaac ttcttcctgc
      541 tcgccttgcg aggaatgaga attactgaca gcgattatcc aatcactgta caatgtcccg
      601 ctgcacgcag gcgagggcgc ggtgacgaaa tatcgccact tggggcgcaa tcagcccggt
      661 ccagagcctg gattcgatcg tacgatacag aagacctgac gaggaaaaat atggcccatt
      721 tatttgatcc acttcccatt cgcggagtca cgttgccaaa tcgcattggc gtctccccca
      781 tgtgccagta ctcaagcgac gacggtttcg ccaacgactg gcatcttgtg caccttggca
      841 gtcgcgcggt gggcggagcc gggttagtat tcgtggaagc gacagccgtc acggcggaag
      901 gccgcatcac gtccgaggat atgggcatct ggaaagacgc gcacatcgat ttcctggcgc
      961 gaatcgtgcg attcatgaag acgcagggca ccgtccccgc catgcagatc gctcacgcgg
     1021 gacgcaaagg cagcacacag cgcccgtggg tgggcaacag caaggtcgcg atcgaggacg
     1081 gcggctgggt tcccgtagcg ccgagcgcga tttctttctc tgagacgtat cccatgccgc
     1141 gtgctctgga aataaatgag attcactcga tcgtggacgc atttgcagct gccgcgcgtc
     1201 gtgcgctgca agccggattt caagtcctgg aaatccatgc cgcgcacgga tacctggccc
     1261 acgaattctt ctcacccctc agcaatttcc gcacggacga atacggcggg tcattcgaaa
     1321 accgcacccg catcggacga gatatcgtca ctgccgtccg caaagtgtgg cccgaaaatc
     1381 ttcccctttt cattcgaatc tccgcgcagg attggaagga aggcggctgg gacctcgatc
     1441 agtccgtgga ccttgccaaa cagttaaaaa aactgggcgt ggatttggtg gactgctctt
     1501 ccgctggcct tgtcgtagat caaaaaatcg cggctggccc gtgctttcaa gtcccgttcg
     1561 ccgaacgcat ccgccgcgat gcaaatatcc tcaccgccag cgttggcctc atcgaaacga
     1621 aagaacaagt ggccgacatc cttgccagga atcaagccga tttggtcctg atggcccgcg
     1681 aatttctgcg cgatccttat tggccgctgc gaaccgcacg tgagttgaag cagcccctca
     1741 cctggccagc ccagtattta cgtgcggctc cagccgattc aaaagcgcga atgcccaccg
     1801 ccgatgcggc atctaaagtg aatgtgctcg attagttggc gatttttatc gtactcgtcg
     1861 gtgtgacgtc caacgctcga tgagccgtcg gcgcgctccc agtgcagttt cgattcatcg
     1921 cacgaggaac gcgctaggcg aactctgtca aaacctctac cataacttgc gtgagtaaac
     1981 acagcgttca tcgccgcgtc ttcacatcta cagggatcgt aatggccggc attctggcca
     2041 gccgcctgct cggattcttc cgcgagtggg cggtggcgca tcaggtcggc tcgaacgcga
     2101 tgaccgatgc gtattacgct gcctttacgc tgcccgattt cttaaattat ctagtggccg
     2161 gagcctccct gagcgccacg ttcattcccg ttttcgccaa atattccgcc gagggcaacg
     2221 aggacgaagc gtggtgggtc ttttcgaccg tgatgacttt catgggcgga ttgctcgcgg
     2281 cattgattct ggccggcgaa atatttgccc cgcaattggc taatctgatc gcacccggat
     2341 tcgctccgcc ggagcgccag cgtctaattt ttctcacgcg cctgatgctc ccggcacaat
     2401 tctgcttcta ccaaggcagc atccttagcg ctgtgcagta cgcaaaggga cgctttgtaa
     2461 ttccttcact ggcgcctgtt gtgtacaacg tgatgattgt cgcgggcggt attttgctgg
     2521 catcgcgata cggcatgacc ggcttcgcta tcggcgttct cgtcggtgcg ctgcttggaa
     2581 attttcttct tcaggtttat ggcgctcatc tggcaggagc gcatttccgt cctaatctca
     2641 atctgcgcca tcctggattt attttatttt tgcgcctctc cgttcccatc atgctggcgc
     2701 tgtctctggt gtttaccgac gactggatca ttcgttggtt cgggtcctat ctgcagcctg
     2761 cctcgatcac ttggctcagc tatggcaaga ccttgatgcg cgtcccactc ggggtcgtgg
     2821 gccagggcgt cggagtcgcg tcatttcctt ttctcgcgca gctttattcc gaaggaaaaa
     2881 ttgatgagtt gaaccgcctg ctcaacacga cgatgaaatg gatgatcgtg ttgttggtgc
     2941 cgatttcggc gctcacgatt gcggaaagtt cgccggtggt ttatctggtg ttttcgcaca
     3001 cgcgtttgcg tggacctgac ttcaatgcta cagcggccac tcttatgtta ttttccctgg
     3061 gcatgttcgc atggggcgcg cagaatattc tggcaagagg cttctatgcc acgcggaata
     3121 cgctgacacc cgccattgta ggcacaatcc tggccatcgc gaatattcct gtgtactggg
     3181 tcctcgtaaa gcactttcag catatcggcc tggctttggc tagctcgctg ggcatcactg
     3241 cttacaccgt agtgctcttc gtgctgttga atcggcggac aaaaaattcc gatgccgcgt
     3301 cgatcacgtt attttttctc aaggtctgcc tggcatcgac cgtggtcgcg tacatttgcc
     3361 accggttgca acgcctactg gaaccgcact tcgcgtggca taccataccg ggtgcattcg
     3421 cacttctgat gatggtgacg tcagcgggaa ttgtgctgct cttcattgcg gcaaaattac
     3481 tgggcatccg cgaatttgac gaacttggcg cccgcgctcg caaactgctt tcccgcaatg
     3541 cacccgaagc cgttgtgcct gtcgcgtcca accatattga cggataagcc attcgaaaga
     3601 gactctggct tgcctgccaa cccgtgcttg atttaaggag aattccgatt aaggctggcg
     3661 gggcggcccc aaggcgtcac gaacgccgcg ggcaagtgta tcgtgcgtga agggcttttg
     3721 cagaacgcgc actgtcgatc cgcgcacgcc atgcttttcg atggcatcgt ccgtgtatct
     3781 ctgcttgttg aaaggcgatc ctccgaatgc tttcgcaaaa ccgcccccga tttttttaag
     3841 aatgtgaaga ggattcgcaa tcaaaaccgt gccgattgca gcgcccagaa tgatgatcaa
     3901 ttccgctggc tgcatcagaa ctctcaaatt gccgtgttcc atcaagtagc cggcagcgac
     3961 gcacccaaag acaatcacga ttccaataat tgaaaacatc gatgtccaat cctccggaac
     4021 agtttccaag tcttatggaa gaacggtgac gcgatttcca cctttggcaa tactttcgag
     4081 gagagatgca tccgcgcgtg caatcaaact ctcaacattg tccccggttc ggcagcccgc
     4141 gccgcctacg ggactcgtta ccgaaaattt atcgccccac cactctatct ttgaccgcga
     4201 aataattcgc ctggctcgct cgccgaccag cggaacttcc atttccttgc aatcggtaag
     4261 aatcgccaaa aattgatatt tcccccagca acctaaaaca tccgtgggcc gcaaacaatt
     4321 ctcgatggat tgtgcgatga tccgcaaaat tgttggcacc acacccggtc cacgcttcgt
     4381 ccggtgctgg tccagctcat cgatttgcag cagcaagatt ccaaacggaa tatgatgctc
     4441 gccgaaagta atcagatttt cgcgaattcg tgtgtccatg aatccctgcg ccgcaattcc
     4501 cgtcaaaaca tcaaggcagc caaaatcggc gaggccggag tggcgtcgcg tggaatccag
     4561 aaacggtatg gttttgtcaa aactttccgc cgccgcgatc accgctccat gactatttcg
     4621 aatggggttt gttcgaagcg tgatagggag acgatatccc tccttgtgaa ggatggaaac
     4681 gcttaggagg gaagattttc cgtcgcgaaa tgcgatgttg atcgggtcga gaggatccag
     4741 atccaggtcg ccggccccgt cactggtggt tagaagatga tcgcgcaaaa aatggccgac
     4801 gacatcctgt ctcaggtagc ctgtaatttt ttccgcggct tcattccaaa agcggatgcg
     4861 ccggttccgg tcaacaatat agactccagt atccagtgcg tccaaaacag tttggtaaat
     4921 cgctggatcg tttagttcgg gcatacgatg actccaattg gaatcatcgg ccaatgaggg
     4981 aaagtagttt aattagactt agaggctttt tcggggattg cttccttgtt ccattttgat
     5041 ttttcgttcg gctcatccca caaacggtag cctcccgtga atgccgtcgc gttgttcccc
     5101 aaccggcatc ccggtggcaa ttctttcaag agttttgcat ttccgccacc cagcaccacg
     5161 taatccgctt ccagcgcatt ttttagatcc tcggcaattt tcgcgacgta tcgtcgccat
     5221 tttttctttc ccaaacgctt taacccgcgc agcccaagat aatcttcata cgtgcgcccc
     5281 tttttgtaag ggagatgcgc gatttccatt gcctccacga cgccgtccac aatcattgtc
     5341 gagccaagcc ctgttcccag tcccaggaaa agcattcttc cgccgtggta gatgccaagt
     5401 gcctgcatgg ccgcgtcatt cacaatccgt accggcttgc caaacgcttt tcggaaatcg
     5461 aatcccaccc agccactcgc aagattatgc ggttcgctta atggatgccc gtgaatcacg
     5521 gggcccgggt acccaattga aacaacggtg tagttccaat ccttcgtcgc ctctcggact
     5581 tctttgacca ttcgtttcgc ggtcatcttt gggccagaag gaattttgat cggctccttc
     5641 tgccccattg ccagcacttt tatatgcgta ccgcccacgt cgacagccaa aatatttcgc
     5701 tgctgttttt ccattgcaac tccttggcat gagacaaatt cgagttagcc tgccccgcta
     5761 aagcatagcg ggaaattctt agccgcctac tttctttttt tcgagttggc cccatcgctc
     5821 gtacaagcgg tccaaaatgt tttgagcctc atccatttcg atgcatgcgg ttttcaggcg
     5881 cgcaggatcg ctggtcaccg cgggatcttc gagcacgacg cgcttggctt caagattctt
     5941 atccgcttcg gcaatgcgtt cttcaatcgt ttcgagctcg cgaacttcgt gataagaaag
     6001 ttttttcttc gaaattgcag attgcgcttc tccctgcacc gtcgcgcgct ttgttccggt
     6061 cgataaattc gtagttccct tttgttgcgc gcgctgccag atttcccact gagaataatc
     6121 ggcaaaacgc tcggctcctc ccagcccatc cagaccgagc acaatggtcg atacccggtc
     6181 gagcatgtac cggtcgtgcg ttaccagcac gagcgccccg cgatattcga gcaaactttc
     6241 ttccagaatt tccagcgtgg gaatatccag atcgttggtc ggctcgtcga gcagcagaac
     6301 gtcagctgct tgcagcatga gttgcgcgat cagcacgcgc gcgcgctctc cgccggaaag
     6361 ctttccaact ggctggctaa ggtgttcagg atcaaacaga aattttgcgg cccaggccgc
     6421 aacgtgaatc acacgatcct gataaatcac cgaatcgcta tccggagcaa gtgcgcgccg
     6481 caggctgatt tccggatcga gttcgcgatt ctggtcaaaa tacacgatgc gcaacgcatc
     6541 ggctctgcgg acttctccgc gagtcgtctc aatatcgccg cgcagcaaac gcagcaatgt
     6601 agtcttgcca cttccgttct gcccaacaag acctactctc atgccggaag tgacagtgaa
     6661 ctggatccca gcgaacaatg tgcgcccgcc aatatcgtac gtcacgccgt ccagggtgat
     6721 caggcgtttg gtttttcgat ccgtcgcgga aaaatcaatt ttggctgagc tcgtcttcgt
     6781 ccgcgaatta aggtccgaca gttcgccgat caactcatgt gcggtatcga tgcgcgcttt
     6841 cgatttcgtt gttctagctt tcggtccgcg gcgcagccat tcaatctccg tgtgcaccag
     6901 gttttcaagc gcttcctgcc gtttgcgctg cgcatgcagg tacacttctt tcgcctccag
     6961 aaacgtgctg tagtttccgt tcacacgcag gtagccgttc tcgtacgagc gagccagctc
     7021 gaccacagcc gtagcgatgt tttcgagaaa atagcggtca tggctgacta cgacgcaggc
     7081 aaacgtcgca ttttgcaaga gtgtttcgag ccactgaatg ccggcaagat ccaaatggtt
     7141 cgtcggctcg tccagcagca aaacgtcagg cgattgcacg agcccttcga cgatcgccag
     7201 cctcttgcgc cagccgccgg acagcgatga cgttagcgcg tccatgtccg tgaaacccgc
     7261 gcgacccacc ggctcggcaa tgcgcgcggc gcgatccttc tccggcactt ctgcctgatc
     7321 gagagcattc gcgatcacgg agcgaatcgt cgccccttcc gcaaattcgg aattctgcgc
     7381 aaccgaacac aggcgcacgc gcttccgaat cgccacgtcc cccgtatcgg gtaccactcg
     7441 gccttgcaaa attgaaagca gggttgattt gcccgaacca ttgggcccaa tcaggccaat
     7501 tcgttcgccc tcagaaaccg taaatgaaat gtcctgaaag agaggttcgg cgccgtatct
     7561 cttggagagt ccttgtgcag cgatgatcgg aggcaaatga ctaagttttc tcccgcgttc
     7621 agtgtagcgt gaaccggaga catccgcaaa atgaagggca ttgagtagcg cgggcttcag
     7681 gctgcggaat ttcgtcctcg taaggcctaa aaccccgcag gctgaaaccc gcgccgctaa
     7741 aaccaatcgc tgcgatacgc gacccaccag cttacgcaga aaagaggaat ggtcgcgccc
     7801 agcgcccacc agaatggcaa cgcaagatcg gcaataatgc caagcccact aaataaggtc
     7861 caaaagattt tgcgttgcat agcaccattg taaactgaaa tttgacgctg agacgatcac
     7921 cgcgccaaaa cagcaaccta ttgagaaacg ataaaggtcc aatgtaggct cgccggaaat
     7981 aaattttcgc acacgctcgg cgagaaattt tccgccgtta ttttttgcga gaacggttag
     8041 atcggctggc ttgtccttcc atgctggcgc agcaggaccg ttccctttgc cttcgctgcc
     8101 atcgcaagca ttacactgct acttgcctgg aggcgtttgt gtcttgtcca ctacgttttc
     8161 ctgaaactct ttcatttcgg agtaagcgca gagggtctcc cccatcggac ccggagagag
     8221 attgtaaagt ttggaagccg agaaggtttt tgtgtaagcc ttagggtcgt cgatcgtaac
     8281 cgtgagctcc agtttttctt ggctcaggcg gcggaatcgt tcgattacgt gcagcgcatc
     8341 gctgtgcgga tgccccgcct ggtccagcca ggtcttgtcg ctcaagccaa tcgtatcgac
     8401 aacaaatgtg tcgccttcat attttccaac agcgtcgccc atccaggtga tatccggatc
     8461 tttaggatgc tcggtattca tggccacggc gcgccatact ttcgtgtact cgtacaagat
     8521 atagacaact ttgggagtct gaataaatgt gatttcccaa gggtacatat aaattcgagg
     8581 aatgcctggg gggtcgcaga atctctgcac gggatcattc gtattgtcga acgtctgccg
     8641 ggccccgaac ggaggtcgcg cgtccttgaa ttttgccgca gcccaaggcg tcataggagc
     8701 cttttccgga tgcttagcaa ataaatctga ggaatcccat gtattgatcc tcacgctttc
     8761 gggtccccgg aagtcaggtt cccacagtcc ggataggtca gggatgccac cggccatcaa
     8821 cccttccttt tgaggttttg tcgcggccgg attcgcgctg gattgagccg acaaactgga
     8881 agaaaagctc agcgccacga tgaccgccag catcccaatt tctagtcgaa ttcgcatctt
     8941 gagttccccc aaggaccatg tccgccaaga atttcgtaac gtgtactatt cgacatggct
     9001 gggaagcgcc ccaccattca ccacggtttt gaggatgaac gtaatcgtgc tgctgattcc
     9061 aaccggcgcg ccatttcgcg aagggtgcgt atcaatcgta atgtcgtcac cggccttcat
     9121 tgcatttttt gtccaaccgg ctcgcaccat gtgaatgggc gcggcaaact cgatggtcca
     9181 gtgttgcgat tcgccgctgt cattttttgc gtccacgtag acaacaacat gaggattgct
     9241 ccagtcaaac ttcgcaaccg tgccgtgcaa tgtaaccgtc ttcgtcatgt cgtatcccgc
     9301 gacaccgtga tgtgccaagg cagcgcccga aaacatcccc aagcttgcga ttgcaaccag
     9361 gatgatgagc agcttccagt tcatgaagcg cctcccgcga tttaccttaa ccaattgttt
     9421 aaccagccat ttggaagtcg aacgatggcg ccatcatagc aggagaaaga aaatttagga
     9481 atgatttgag cgggttatgt agcgcgggct tcagagtctg cgcgagaact gcagaatcgt
     9541 tgtgggtcgt ggctttagcc acgacataag tccggcaaaa tgaatgcgac tagaagctgt
     9601 tgcacaacct aggacaagcc attgaaatgg ctatggttga atttttgaaa atgggagata
     9661 agcaagatca gcagcgccga aggagggtcg atggcgcgat ctggtccgtt gctgaccgaa
     9721 gcgcagtgga agaaaatcgc ccctttactc ccaaaaccgc ccaaacaacg taagggcggt
     9781 cgtccgtgga tcgagaaccg tcgtgtgctg gaagggattc tgtggattct gcggagcgga
     9841 gctcgctggc aggatatgcc ggagaaattc ccgcatcctt cgacgtgctg gcggcggctg
     9901 cgagactggg aggagcgggg cgtttggttg aaaatctggc ggtcgtttct gagcgagttg
     9961 aacgaacgcc agcagttgaa gtggagcgaa tcgtttctgg acgggagttt tgctccggcg
    10021 aaaaaggggg ctgcggagtc ggaaaaacca agcggggcaa ggggacgaag tggatggtgg
    10081 tggtcgacgg ccgcggtctt cctctgggaa actaccttca ctctgcatcc ccggcggaag
    10141 tcaagctcgc ggagacgacg ctcgcggcga tccgtgtagc acggagccat cacgcgggct
    10201 gtccgcggca gaagcctgtg cgggtgatcg ccgacaaagc ctatgacagc gatccgctgc
    10261 ggaagcgctt gcagaagcgg gggatcgaat tgatctgccc gcacaagaga aatcgcgttc
    10321 ggcccgcgac gcaggatggc cgagcgttgc ggcgctacag gcggcgatgg attgtcgaac
    10381 gtaccaacgc ctggatcgga aacttccggc ggttagttgt gcgctacgac cgctcgctca
    10441 ccatctatgg cgcattcttt catatcgcct gcttcatgat cgtcttacgg agggttgtgc
    10501 aatagcttct agtgaaaact caggttgtgc gcgccgcaga aaatcgagct gacgaagctg
    10561 gcgccttgcg gaacccacca catatggcga acccaggaat agcggctata catcattttt
    10621 ttgcccgcaa aatccataag c
//
LOCUS       DASZMB010000247         8308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_811169, whole genome shotgun sequence.
ACCESSION   DASZMB010000247 DASZMB010000000
VERSION     DASZMB010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 8308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8308
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811169"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VG272_11580"
     CDS             complement(<1..497)
                     /locus_tag="VG272_11580"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HWF14370.1"
                     /translation="MKLFRMLLVLAAMCASTFALAQEPAQQNPKKGTKEWTELEKTLW
                     DVDQQWLCSAGATPYHQDYKECIEFRSKFWTDQFFEVGTKGQVQVKSEMIAHQRVAHP
                     SKGVGPHPDSFKLMAVYGNVALATDHTFLQDESKDGKVTGTDTTVLRMFAKEDGKWRP
                     AGGALV"
     gene            complement(568..1536)
                     /locus_tag="VG272_11585"
     CDS             complement(568..1536)
                     /locus_tag="VG272_11585"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HWF14371.1"
                     /translation="MAEKKIITVFGSTGAQGGGLARAILRDPQSGFTARAVTRDVNSD
                     KAKELASMGAEVVAADVDDPASIQKAFEGAYGAYCVTFFWAHFSPEKEMAEAQAMATA
                     AKKANLKHVIWSTLEDTRKWCPLSDPSIPTLHGKYKVPHFDAKGESNHFFTDLAVPTT
                     FLLTSFYWDNFIYFGMGPQKGPDGKLAITFPMGDKPLPGIAAEDIGKCAFGLFKRGNE
                     FIGKTVGIVGESLSGAQMASAFAELMGQPVVYNSIPPEVYRSLGFPGADDLGNMFQFK
                     RDFNDYFCGVRDAAFSKSLNPELQDFKTWLSNNKTAITKTQTAAGA"
     gene            1999..3282
                     /locus_tag="VG272_11590"
     CDS             1999..3282
                     /locus_tag="VG272_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14372.1"
                     /translation="MQLKAVLLGLAASSLLMTLSARAQMQLGEREAVDMPPGDAKELV
                     ESSCSVCHSLTNITKSQGHTPEEWKTTVSMMRNVGAAVPDDKVEMVTNYLIKNFPEKP
                     GPKPAAIPGNVEVSFKEWKLPTPGTRPHDPLAAPDGTIWFTGHMANLLGHIDPKTGDI
                     KEYRTGKPMSGPHGLVMDKQGNIWFTANFKAYIGKFEPATGKFTEYPLDRNARDPHTP
                     LFDQKGTLWFTVQGANMVGRLNTETGVSKVVTSPTPKSNPYGMVVTTKGVPYYVEFGA
                     PKIASIDPETMDIHEYNLPNPDARPRRVAIMPDDTLFYTDYGRGYLGEFDTKTGKMTK
                     EWLSPSGPQSRPYGITIVDGIVWYSEAGVKPNTLVRFDPKSEKFQTWTIPAGGGVVRN
                     MMHFPDGKLVLTESGENVVALVTVKGGEKMAMSAK"
     gene            3715..4146
                     /locus_tag="VG272_11595"
     CDS             3715..4146
                     /locus_tag="VG272_11595"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWF14373.1"
                     /translation="MMNPRIRTAAMVAALLVSGATAAMAQDTVKKSTIPMTSASSGQE
                     MFNTYCAVCHGTSGKGDGPASSEFKIAPANLTMLAKNHNGTFPEAYVSQVIQTGPRDA
                     KAHGSKDMPVWGRLFASLGDDATVKLRVHNLTTYIGSLQAK"
     gene            complement(4305..6686)
                     /locus_tag="VG272_11600"
     CDS             complement(4305..6686)
                     /locus_tag="VG272_11600"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWF14374.1"
                     /translation="MKNPSQKASESEMVQDAADALERAGFSRRNFIKGTGALIVGFSM
                     GGAVEAFAAQGRPAVFGENASPDSPPAAEVDSWIAIGSDGTVTAYTGKEELGQGMSTA
                     QTQLVAEELCVPFLRVNLIVADTAKTPDQGVTSGSQSHPANFNRNNLAGACATARQAL
                     LDLGSKKLGIPAEQLVAVDGTVRAKNDATKKVAYADLVAGKKFSLKVDSSAKRKPASE
                     WTVLGKPLGRPDMTDMVMGTFEFAHNVKRPGMLHGMVIRPSAVGANLMNVDESSVTGM
                     PGLVKIVVKKNFVGVVAEKPWQAIQIAKRLSVTWSPAPELPKQETFYEHLRTQRPVRD
                     ILLVNSKDVDQKLAGAATVVKATYLHPYQMHGSVGSSCAVADVQGDKATVWSATQGVW
                     HLRATLAMLLELKPENVHVIFKRGSGCYGINGADAVSYDAAMLSQAVGKPVRIQLTRK
                     DEMAWENYGFAFTIDERAGLDAQGNIVAWDHEAWSPVLGNRPVYTYPGNVITGFLVGF
                     QPEEFKPRTPAPEPTSFSNNSNAIPSYVVGHVGGADHGAGTVKSERALLHNVISPFWT
                     GPLRSPQRLQNTFAHESFMDELAAHAKADPVEFRLRHLSEPRMVDVVKAASVNAKWDA
                     RPSPNPHAKSAAGFATGRGISCVAYEGDNGFSAMVAEVQVELATGKILVKRLVVAIDA
                     GPVSNPDGLRNQSEGGALQGLSRALGEEVTWDDHNVTSVDWKTYHSLPLGFAVPKVDV
                     VLLNRPDLPATGAGETAITVVAGAIANAIFDATGARIRQVPFTAERIKAALAG"
     gene            complement(6683..7150)
                     /locus_tag="VG272_11605"
     CDS             complement(6683..7150)
                     /locus_tag="VG272_11605"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWF14375.1"
                     /translation="MSAISLKVNGKTHSLDVDPTTPLLYVLSDDLGLHGPKFGCGLGQ
                     CGACTVLVKGQAVRSCITAVEAVQGIEIVTLEGLGTPERPHPIQKAFIDEQAAQCGYC
                     LSGVILTAKAFLDKNPKATEKEIQEGMSRVLCRCFTHTRMLRAIGNAQKEMAR"
     gene            complement(7341..7994)
                     /locus_tag="VG272_11610"
     CDS             complement(7341..7994)
                     /locus_tag="VG272_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14376.1"
                     /translation="MKNLIGIFLACAIAAIPAAAQNHGGGPARGVGGGHVPSHGPAPH
                     PGSPPTRRSAPPQQQSRPAPAQEQSRQAPAQEQSRQGPAQEQSRGNQEPRRTFSDKQG
                     HPEAPHVHSRGDKWVGHDTGRDDVHYHVDRPFEHGHFSGGFGRGHVFHLQGGGPDRFW
                     FNGFYFSVAPYDFDYANGWLWNSDDIVIYEDPDHDGWYLAYNVRLGTYVHVMYMGNG"
     gene            8113..>8308
                     /locus_tag="VG272_11615"
     CDS             8113..>8308
                     /locus_tag="VG272_11615"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HWF14377.1"
                     /translation="MQSNFAAQYCRTEDSDVGKFFPFVHTTLSKNYVGSRVPTMKLDK
                     IYTRTGDEGKTSLGDGSRLPK"
BASE COUNT         1859 a         2399 c         2146 g         1904 t
ORIGIN      
        1 acgagtgcgc cgcccgcagg tctccacttc ccatcctctt tggcaaacat gcgcagaacc
       61 gtggtgtcgg ttccggtcac ttttccgtcc ttcgactcgt cctgcaggaa tgtgtgatcg
      121 gtggccagcg ccacatttcc gtaaaccgcc atgagcttga aactatccgg gtgcggtccc
      181 actcccttgg agggatgagc cactcgctgg tgagcgatca tttccgattt aacctgcacc
      241 tggcccttcg tgcccacttc aaaaaactgg tctgtccaga atttgctgcg gaactcgatg
      301 cactccttat aatcctgatg atagggagtg gcgcctgcgc tgcaaagcca ctgctgatcc
      361 acatcccaca gggttttctc gagctccgtc cattccttgg tgcctttctt gggattctgt
      421 tgagccggct cctgggccaa tgcgaaagtg gatgcgcaca tagcggcaag aacaagcagc
      481 atgcgaaaca acttcatggt tgtcgtcctc ctgattattt gtgcttccaa aaattaaatc
      541 gtaattactg cgctgaatct ctgacgatta tgcgccggcc gctgtctgtg tttttgtaat
      601 ggcagttttg ttattgctta gccatgtttt aaaatcctgc agctccggat tcagggattt
      661 tgaaaacgca gcgtcacgta ctccgcagaa ataatcgtta aaatcgcgct tgaattgaaa
      721 catgtttccc agatcgtccg cgcctggaaa tcccaaggac cgatacacct caggcggaat
      781 actgttgtag acgacaggct ggcccatgag ttcggcgaag gccgaggcca tttgcgctcc
      841 actcaagctc tccccgacaa ttcccactgt cttgccgatg aattcgttac cgcgcttgaa
      901 tagaccgaaa gcgcatttcc cgatgtcttc cgcggcaatt cccggcagag gcttatcgcc
      961 catcggaaaa gtgatcgcca acttcccgtc cggacctttt tgaggaccca tgccgaaata
     1021 aataaaattg tcccaataaa aagatgtaag cagaaacgtc gtgggcacgg cgagatccgt
     1081 gaagaaatga ttcgactccc ctttagcgtc aaaatgggga actttatatt ttccgtgaag
     1141 cgtggggata ctcgggtcag acagcgggca ccacttgcgc gtgtcttcca gcgtggacca
     1201 gatcacgtgc ttgagattcg ccttttttgc ggcggtggcc atggcctgag cttcggccat
     1261 ttctttttcc ggggagaaat gcgcccagaa aaaagtaacg caataggctc cgtacgctcc
     1321 ttcgaaggcc ttctgaatgc ttgccgggtc gtcgacatcc gcggcaacga cttcggctcc
     1381 catgcttgcg agttctttag ccttatcgct gttcacgtct cgggtgactg cgcgcgctgt
     1441 aaaaccgctc tgtggatcgc gtagaatagc tcgcgccagt ccgccgccct gcgctcccgt
     1501 cgatccgaac acggtgatga tcttcttttc agccattatc gcctccgagg aattaaaaac
     1561 tgcctactca catatgaacg gctgcgccga tcatatcatt ccattcgaga gacttcgcgg
     1621 gcgggagatt ttagtatggg ctgtggaaaa aaacaaaaaa cttaataatg cttatgcctt
     1681 tcggggattg aaacatataa ccgtatctgt tataacgatg cagttactta acgggggcgg
     1741 gagtaccgtg actctaaacg gcaagctaac ccgccccgcc tccgtccagt tcctatccaa
     1801 atagtttccc cttcggatgt gcaaaactaa cctttttcct aaatttccgc atgctttgct
     1861 cttgtctctg tcgcgctagt caacgaccac gccttggaat acccgccagc aagagagccg
     1921 cggccaccaa catttttgta acgcaacgga gagattctta atctatgata aaggcgacgt
     1981 tagtccccgg aggactttat gcaattaaaa gcggttttgt tgggcttagc ggccagttcc
     2041 ctgctgatga cgctgtccgc ccgcgctcaa atgcaattgg gcgagcgtga ggctgtcgat
     2101 atgccccccg gggacgccaa ggaactggtc gagagttcct gctcggtgtg ccacagcttg
     2161 acaaatatca caaagtccca gggccacacg ccggaggaat ggaagacgac agtttcaatg
     2221 atgcgcaatg tgggcgcagc cgttcctgat gacaaagtag agatggtaac gaactacttg
     2281 attaaaaatt tccccgagaa acccggcccc aagccggcgg ccatccccgg caacgttgag
     2341 gtttcgttta aagaatggaa attgccgact cccggcacgc ggccacacga tcctttagct
     2401 gcgccagacg gcacgatctg gttcaccggg cacatggcga atctactggg tcacatcgat
     2461 cccaaaaccg gcgacattaa ggaataccgc accggaaagc cgatgtccgg gccccacggg
     2521 ctggttatgg ataaacaagg aaacatctgg ttcaccgcaa atttcaaggc gtacatcgga
     2581 aagtttgagc cggcaacggg gaaatttacg gaatatccgc tcgaccgcaa cgcgcgcgat
     2641 ccccacacgc cccttttcga tcagaaaggc acgctatggt ttaccgtgca gggcgcgaat
     2701 atggtgggcc gcctgaacac ggaaacggga gtctcgaaag tagtgacctc gccaactccg
     2761 aaatcaaatc cctacggcat ggtcgtcacc accaaaggtg ttccctacta cgtcgaattt
     2821 ggcgcgccca agattgccag catcgacccg gagaccatgg atattcacga atataatctt
     2881 cccaatccgg acgctcgtcc acggcgcgtg gcgatcatgc cggacgacac gctattttac
     2941 acggactacg gacgcggata tctcggggag ttcgatacca agactggcaa aatgaccaag
     3001 gagtggctct ctccaagcgg tcctcaatct cgcccatatg gaattaccat tgtcgacggc
     3061 atcgtgtggt acagcgaggc aggtgtaaag ccgaacacac tcgtgcgctt tgacccgaaa
     3121 tccgaaaaat ttcagacctg gaccattcct gcgggcggag gagtcgtgcg aaacatgatg
     3181 cacttccccg acggtaaact tgtgctgacg gaaagcggtg aaaacgtggt cgcactagtc
     3241 accgtaaagg gtggtgagaa gatggcgatg agcgcgaaat agacaagcgg ttcgcgattc
     3301 gtggccgcga gtcacgattt tgttccagtt tgaaacgccg gggagatatc gggacgtcag
     3361 atcccgactt cccggcgttt attcgtctcg gttcttatta gtccaatcga ttcgtcctcc
     3421 gcgattcaga taatccaaaa tgtctcgtca attttgtgcg gatagtcaca atcatgagtg
     3481 atcccaaaca cagcgacgcg tgagaccacc atggtaaaca tgtcgtcata agccatggcc
     3541 ccgacgccat cagtttaatc ctgtttcctt tcgatcaaat tgaaggcgcc cgcggcgggc
     3601 tattgcgaat ccttgggccg accctgatca acagacctgg aaatttcaga gcgtttctga
     3661 atcagcgcct cgctcaaaag atttgaattt caaatgcgaa atgggaggca aacgatgatg
     3721 aacccacgta ttcgtactgc ggccatggtg gcagcactgt tagtctcagg tgcgacggcc
     3781 gcgatggcac aagatacagt caagaagtcg accatcccga tgacttccgc ttcctcgggc
     3841 caggagatgt tcaatactta ctgcgcggtt tgtcacggaa caagtggcaa gggtgatgga
     3901 cccgcttcca gcgaattcaa aattgccccg gccaatctga caatgttggc aaaaaatcac
     3961 aacggaactt tccctgaagc ttacgtttca caggtaattc aaactggtcc tcgagatgcc
     4021 aaagcgcacg ggtcaaaaga catgcccgta tggggacggt tgttcgcttc gctgggcgat
     4081 gacgccacag tgaaactacg cgttcacaac ctgacaacct atattggatc gttgcaagcg
     4141 aagtaaagct tttcaggatt ccgaagccat gccgaaattt ggaagggatg caggatttca
     4201 tagaacatct taaattatag ttcctgcatc ccggttcaaa aacttccctg cgatccagcg
     4261 acgacgcgcg aaatggatcg cagtttttct ttcgtctcag acagttatcc ggcgagcgcg
     4321 gccttgatgc gttcggcagt aaacggaact tgacggatgc gcgctcccgt tgcatcgaaa
     4381 atcgcattcg cgatcgcgcc ggcgaccacc gtgatcgcag tttcgccggc tcccgtggcc
     4441 gggaggtccg gccggttgag tagcacaacg tcgaccttag ggaccgcaaa tcccagcggc
     4501 aaggaatgat acgttttcca gtcaaccgaa gtcacattgt ggtcgtccca ggtgacttcc
     4561 tcgccgagag cgcgactcaa tccctgcagc gcgccgccct cagactggtt ccgcaagcca
     4621 tcgggattgg agaccggccc cgcgtcgatg gcgacaacaa gcctttttac taaaatcttt
     4681 cctgtcgcta attccacttg aacttccgcc accatggctg agaaaccgtt gtcgccttcg
     4741 taggccacgc aggatatgcc gcgcccggtc gcgaaacccg ccgcggattt cgcatgtgga
     4801 tttggcgaag gacgcgcgtc ccatttagca ttcactgaag cggccttcac cacgtccacc
     4861 attcgcggtt cactcaagtg gcgcaaacga aattccacgg gatcggcttt cgcgtgcgcc
     4921 gcaagttcat ccatgaacga ttcgtgcgca aatgtatttt gcaggcgctg aggtgaccgc
     4981 agcggtccag tccagaatgg cgaaattacg ttgtgcagca gcgcgcgttc agacttcacc
     5041 gttcccgcgc cgtgatccgc acccccgacg tggcccacca cgtatgacgg aatcgcattg
     5101 ctattgttgc tgaatgaagt gggctcaggc gcgggcgtac gaggtttgaa ttcttcgggc
     5161 tggaatccca ctaaaaatcc agtaattaca tttccgggat aagtgtacac cgggcgattt
     5221 cccaacacgg gagaccacgc ctcatggtcc cacgccacaa tatttccctg cgcgtcaagt
     5281 cctgcgcgct catcaatagt gaatgcgaag ccatagttct cccacgccat ttcatccttg
     5341 cgcgtgagct gaatgcgcac cggtttgccg acggcctggg aaagcattgc cgcgtcgtag
     5401 ctcaccgcgt ctgcgccatt aattccgtag cagcccgatc cgcgcttgaa gatcacgtgc
     5461 acgttttccg gtttcaactc gagcagcatg gccagagtcg cacgcaaatg ccacacgcct
     5521 tgcgtagccg accacaccgt cgccttatct ccctgcacgt cggccacggc acacgaactg
     5581 cccacagatc cgtgcatctg atagggatgc aagtaggtcg ctttcaccac ggttgcggcc
     5641 ccggcgagtt tttggtccac gtctttggag ttgaccaaca gaatgtcacg cacaggcctc
     5701 tgcgtccgca gatgttcgta aaacgtttcc tgcttcggta attccggcgc gggcgaccac
     5761 gtcacgctca gtcttttggc aatttgaatc gcctgccacg gtttttcggc caccacgccg
     5821 acaaaattct ttttcaccac gatttttacg agtcctggca tgccggtcac cgaactctca
     5881 tccacattca tgaggttcgc gcccacggcg gacggtcgaa tcaccattcc gtgcagcatt
     5941 cctggccgct tcacgttatg cgcaaattcg aaggtgccca tcaccatatc ggtcatgtcc
     6001 ggtcgcccaa gcggtttgcc gaggacagtc cattcgctcg cgggcttccg ctttgctgaa
     6061 gaatcaacct tgagactgaa ttttttccca gccaccaggt ccgcgtaggc gactttcttc
     6121 gtcgcgtcgt ttttcgcgcg aactgttcca tcgacggcga ccaattgctc cgccgggatg
     6181 cctaattttt tagacccaag atccagaagc gcttggcgcg ccgtggcgca agctcccgcg
     6241 agattattac gattgaaatt cgcgggatgc gattggcttc cggaggtgac gccctgatca
     6301 ggggtcttgg cggtatctgc aacgatcagg ttaacgcgta gaaacggcac gcaaagctct
     6361 tcggccacca gttgcgtctg ggccgtcgac attccttgcc ccaattcttc tttgcccgtg
     6421 taagccgtga cggttccgtc ggacccgatg gcgatccacg aatctacttc cgccgcgggc
     6481 ggagaatccg gcgacgcatt ttctccgaaa acagctgggc gtccctgcgc cgcgaacgcc
     6541 tccaccgcac cgcccatact gaatcctacg attagcgcac ccgtcccttt gataaaattg
     6601 cgccgcgaaa atcccgcccg ttccagcgcg tccgccgcgt cttgcaccat ttccgattcc
     6661 gaggctttct gagatgggtt tttcatcgcg ccatctcctt ttgcgcattc ccaatcgcgc
     6721 ggagcattcg cgtgtgcgtg aaacacctgc acaggacgcg cgacattccc tcttgaatct
     6781 ctttttctgt cgctttggga ttcttgtcga gaaaagcttt ggccgtcaga atcacgccgc
     6841 ttaaacaata gccgcattgc gcggcttgct catcgataaa cgctttttga atcgggtgcg
     6901 gtctttccgg cgtccccaat ccttccagcg taacgatctc aattccctgc acggcttcca
     6961 ccgccgtaat gcacgaacgc accgcttgcc ccttcaccag caccgtgcac gcgccgcact
     7021 gcccaaggcc gcagccaaat ttcgggccat gcaggccgag gtcgtcgctc aacacgtaga
     7081 gcagcggcgt agtcggatca acgtccaggc tatgcgtctt gccgttgact tttagtgaaa
     7141 tggcgctcat atgtcctcaa acttgttgcg caaggtgtat ttcgacaagt gtatgccttt
     7201 tcggatggcc gatgcaattc ctgactggag tgcagcagta gatgtacgcc tccgcaaaat
     7261 gaaaaaggcc gaatcgaaat tcggccctct tcgtttccgc gcgttgccgg atcctttacg
     7321 ttcctccgga acgatatgct ttagccgtta cccatataca tcacatgaac gtatgttccg
     7381 agtcgcacgt tatacgccaa gtaccagcca tcatgatccg gatcttcgta gatcacgatg
     7441 tcatccgagt tccagagcca gccgttcgcg taatcgaaat cgtacggcgc gacggaaaaa
     7501 taaaaaccat taaaccagaa acggtccggt ccaccgcctt gcaagtggaa cacatggccg
     7561 cgaccgaatc cgcctgaaaa atgtccgtgc tcgaacgggc ggtccacatg gtagtgcacg
     7621 tcgtctcttc cagtgtcatg cccaacccac ttgtcgcccc tggaatggac gtgcggcgcc
     7681 tccggatgcc cttgcttgtc gctgaacgtg cggcgcggct cttgattccc acgggattgc
     7741 tcctgcgcgg gaccttgccg cgattgttcc tgcgcgggag cttggcgcga ttgctcttgt
     7801 gcgggcgccg ggcgggattg ctgctgaggg ggagcggaac gccgcgtagg tggcgagccc
     7861 ggatgcggcg cgggtccatg cgaaggaacg tgcccgccgc cgactccgcg cgccggtccg
     7921 cctccatgat tttgagccgc cgcaggaatc gcggctattg cgcacgccag aaatatacct
     7981 atgagatttt tcatttttaa actcctggaa gacgatttgg aattacgcca gaacggaagt
     8041 gagctggacc taggtagtca attctgcgct caatcagcat taatacttaa gatggctgcg
     8101 ggagatgttc ggttgcaatc aaatttcgcc gcgcaatatt gcaggactga ggatagcgac
     8161 gttggaaaat tctttccctt cgtgcacact acactcagca aaaattatgt tggatccagg
     8221 gtgcctacga tgaagcttga caaaatttat acacgcaccg gcgacgaagg aaaaacttct
     8281 ctcggcgacg gttcgcgcct gcctaaat
//
LOCUS       DASZMB010000248         2705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_5190893, whole genome shotgun sequence.
ACCESSION   DASZMB010000248 DASZMB010000000
VERSION     DASZMB010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5190893"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(391..1650)
                     /locus_tag="VG272_11620"
     CDS             complement(391..1650)
                     /locus_tag="VG272_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14378.1"
                     /translation="MEWYYPLVRHHLQHIHPHLWAFFKTFRKWLVSLFLASVFFFATL
                     FELEISARARAASPPPLKDHYLMLVNTAMARQMDAQKLAQFDRSPYEGLAVSFSDAYD
                     VSPVPTPASMELQIAGWKKFTGKHIWPWVYLNRMIGANNAEGNELTQVPYFQKFQGLD
                     LDGTAGAQNDFLENWRNALRVARKISVPGIVFDLEFYNNYKADDISDLARMTSMSREE
                     TVQALKNLGARMAETASAEYPHATIWVLFTGFTRPDYRIIDGQPHFLAGTYIVEGLLD
                     EIQQHQLALHVLSGGELGLGYCHNSIDDLRQAIQKRAQIYAPLLQKYKGILELSGTMT
                     LWSDRSAKKNWVAEEACGNSSAATLEDLIPYMELLFRSYRYNWIYGSPNGGYLALNSA
                     IAPRFDAAIVRAKALAIASETAIHPDP"
     gene            1912..2694
                     /locus_tag="VG272_11625"
     CDS             1912..2694
                     /locus_tag="VG272_11625"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWF14379.1"
                     /translation="MLLTEKVAVITGGGRGIGRAIALRFAGAGAAVVLGARTVSEIEA
                     VAREVRDAGGRAAAVPADVADEKHCEMLIQAAESQFGKADILVNNAGEYGPVKPIEEI
                     TPSEWDRVIAVHLRGAYLLTRLVLPKMYARGSGAILNISSLSAKSAFGWGSPYASAKA
                     GMLGMTRVVAAEAARKGVRVNAICPGPVTETKMSKDLGQTLATRLGVSKEDQLKGFLS
                     TILQGRGQTVNEIAAAALFLCSDEASAITGQALNVDGGAAFC"
BASE COUNT          639 a          683 c          764 g          619 t
ORIGIN      
        1 tgttgaattc tgaatggatc cacaaaacca atgacaaaag cgaccatcga tttacaacca
       61 aagaacctag cgtctatttt tcgaggactc tatgtccgtg tggcctctcg aattggagtt
      121 gatccatctt atgtgagccg agttgcgcga ggagaacgca aatcgcaggc tgtcgaggcc
      181 gcactcgaac gcgaaacgca caaaatcatt gggcagatcg caaaacaatt gaaagtgaat
      241 cacaacggtt cagggcgcgg atcaacgaat cggaatggcg cacgtcgcgc cggggcaaat
      301 cgacgcgcgg tcaaagcatc gaaattctct cgggactgaa gcgccatgtg ccggcttgct
      361 tctgtggctg ggcatcatgc caattccgat tcaagggtcg ggatggattg cagtttccga
      421 ggcgattgct agcgcttttg cccgcacgat cgccgcgtca aaacgcggcg cgattgcaga
      481 attaagcgcc aggtaacccc cattgggcga accgtaaatc cagttgtagc gataggaccg
      541 aaataagagt tccatgtatg ggattagatc ttcgagcgtt gcagccgatg aatttccgca
      601 ggcttcctca gcaacccaat ttttctttgc ggacctatcg ctccaaagcg tcatggttcc
      661 gctcaattcc agaatgccct tatatttctg caggagggga gcgtatattt gggcgcgttt
      721 ttgaatagct tggcgaagat catcaattga attgtgacaa tagcccagac ctaattcccc
      781 gcctgacaaa acatgaagcg caagttggtg ctgctgaatt tcatcgagta gtccttcgac
      841 aatatatgtg ccggcaagga agtgtggctg cccgtcgatg attctgtaat ccggacgcgt
      901 gaatcctgta aacaagaccc aaatcgtggc atgcggatac tccgccgaag cagtttccgc
      961 catgcgcgct cccagatttt tcagtgcttg caccgtttct tcccgtgaca tggacgtcat
     1021 gcgcgcaagg tcggaaatat catccgcttt gtaattattg taaaactcta gatcgaaaac
     1081 aattcccggc acgctgatct tgcgtgcaac gcgaagtgca ttgcgccaat tttcgagaaa
     1141 atcgttttgc gcgcccgccg tgccgtccag atccaatccc tgaaattttt ggaaatacgg
     1201 aacctgggtg agttcatttc cttccgcgtt gtttgctccg atcattcgat tcaggtaaac
     1261 ccacggccag atatgtttgc cggtgaattt cttccagccc gcgatctgca attccatcga
     1321 cgcgggagtg gggactggtg aaacgtcgta cgcgtctgaa aatgaaacgg ccaacccctc
     1381 gtagggcgag cgatcaaact gtgccaactt ctgcgcgtcc atctggcgag ccatggctgt
     1441 gtttaccagc atcagatagt gatctttcaa cgggggaggc gaagcggcgc gcgcacgtgc
     1501 gctgatttcc aattcaaaaa gtgtggcgaa gaagaagacc gaagccagaa ataatgagac
     1561 cagccatttc ctgaacgttt tgaaaaatgc ccacaggtgt ggatgtatgt gctgcaggtg
     1621 gtggcgcacc agcggataat accattccat cgtgttcgct cctagtcaca cttcgtgaca
     1681 tcgtcacaac ctagcctggc gaaaagtctc aatttagttc tgaaggcagg caaggttttg
     1741 tggcgttggc ggttttggtc cggcctattt cacaaaaaca cacgcatcac cgaacccatc
     1801 gttaaatgag acagaaagtt gcagaaatcg cggcacataa acatcttctt tgacgcttct
     1861 ttggagagaa ccttcgcaaa tgctatgctg cccattcgat cggagggttt tatgttgctt
     1921 actgaaaaag tggcggtaat tactggcggc gggcgcggga ttggacgcgc gatagcgcta
     1981 aggttcgcgg gagcaggtgc ggcggtggtg cttggggcgc ggacggtttc ggaaattgaa
     2041 gccgtggcgc gcgaagttcg agacgctggc gggcgcgcgg cagcggttcc tgcggacgtg
     2101 gccgacgaga aacattgtga gatgctgatc caagcagccg agtctcaatt cggaaaagcg
     2161 gacatcctcg taaacaatgc gggggaatac ggccctgtaa aacccatcga agaaatcaca
     2221 ccctcggagt gggatcgcgt gattgccgtt cacctgcgag gcgcttacct gttgacgcgc
     2281 ctcgtgctgc cgaaaatgta tgctcgcgga tcgggtgcga ttttgaacat ctcaagcctg
     2341 tcggcgaagt cggcatttgg gtggggctca ccctatgctt cggcgaaggc agggatgctg
     2401 ggaatgaccc gtgtggttgc ggccgaagcg gcccgcaagg gcgtgcgcgt gaatgcgatc
     2461 tgcccgggac ctgtcacgga aacgaagatg tcgaaggact taggacagac cctggcaacc
     2521 cggctgggag tttcaaagga agatcagctt aaaggcttcc tgagcacgat ccttcaaggc
     2581 cgcggccaga ctgtcaatga gatcgccgcg gcagctcttt ttctttgctc agacgaagcc
     2641 agtgcgatta ctgggcaggc tctgaacgtg gacggcggag cggctttttg ctgatctctt
     2701 cggct
//
LOCUS       DASZMB010000249         6397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_813121, whole genome shotgun sequence.
ACCESSION   DASZMB010000249 DASZMB010000000
VERSION     DASZMB010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813121"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VG272_11630"
     CDS             <1..1107
                     /locus_tag="VG272_11630"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HWF14380.1"
                     /translation="AVVAIAYQSRHKIHLADFTWNKFVASVGQANIFLLFLCLVGIYG
                     CYALRAVRWQRFCKYVGPTNFLNTYAGTLMGFAAIFVLGRAGEPVRPLLLARKERLPV
                     SSLFGIFFLERFCDFAAAAALACLSLLVFPNRLSDAGADMGWVESARHGGWLLLAAMT
                     ALIVMLVVYRLHGAAILDRILARWRDAGGFRSRFASGITGISEGLQAIRTTSDLLAAI
                     FYTAAHWVLATLIYLGVAKAFGASFMHSDMNFSGAMLLLAVTLVGSVLQLPGVGGGAQ
                     IASFIGLTKIFGVEQEPAAAMAVVLWLITFAGGTLAGIPLLIHEGMSVGELRQLARAE
                     AEAEESGKHIAVTEDNGVSPSSRAAKAKPLEEKP"
     gene            1107..1571
                     /gene="nrdR"
                     /locus_tag="VG272_11635"
     CDS             1107..1571
                     /gene="nrdR"
                     /locus_tag="VG272_11635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HWF14381.1"
                     /translation="MKCPFCSVMEDKVIDSREGRAGDLIRRRRECLKCGRRYTTYERI
                     DEIPYMVIKKDGRRERFDRQKILQGLLKSCEKRPVATAKLEALVDEVERFVHEAPERE
                     RKTSEVGELLMNRIKKLDKVAYVRFASVYLDFKDVKEFMSELKGLLKDRSKK"
     gene            complement(2144..2704)
                     /locus_tag="VG272_11640"
     CDS             complement(2144..2704)
                     /locus_tag="VG272_11640"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HWF14382.1"
                     /translation="MGNRESLLRYFLAIVLVGSLGLVWSAPNVLASATTSHKVLSLSD
                     QVHHELAMLPWYGVFDNLEYDVNGTEVVLKGQVISEHATTKDDAERFVDSIPGVTNVI
                     NRIEVLPPSQFDDQIRRAEYRTVFSKADLGRYTMGAIPQVHIIVRNGHVSLDGVVMNQ
                     MDKNIAGIVANSVPGVFSVENNLRIG"
     gene            2895..3644
                     /locus_tag="VG272_11645"
     CDS             2895..3644
                     /locus_tag="VG272_11645"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HWF14383.1"
                     /translation="MQSKSIWVIAHRGASGHAPENTMASFRRAVEFGARFIETDLQLT
                     RDARVIAIHDLTYDRTSTGKGDVHDLPLKQVRALDAGSWFGDREGKSYAAEKVPVLEE
                     ILDFAEKNDVIFYLEIKSGQAWGIEHVVVATLRDRNASARVVIISFDPTTLDAVQRLD
                     STMMTGLLCEIPSSDLVERTVRAGARQLILRVDLITSAVTEKAHRAGLQVVAWTINDV
                     EQMRTTIAAGVDGIITDYPDRLLELLRESAE"
     gene            complement(3717..4490)
                     /gene="rsmA"
                     /locus_tag="VG272_11650"
     CDS             complement(3717..4490)
                     /gene="rsmA"
                     /locus_tag="VG272_11650"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HWF14384.1"
                     /translation="MSPERLGQHFLADRDWQEQIADAVCMGSGVWVEIGAGHGEMTTR
                     LAQNASKLFAVELDPPLARRLRQSTAHLNNVEIVHSDVMAVDFEKLTRGERFSVYGNL
                     PYYITSPILHRLFEHAERIAAIHIVIQLEVAERIVAQPGRRDYGYLSVASQWYGTPSI
                     EFRIPPEAFSPPPKVDSALVSFHMPGARVSNPVPGEHAFLEFVKECFAQKRKTLRNNL
                     RQGLGPRTEETITAAGLPSDARAEQLTIPQFVNLFQLAK"
     gene            complement(4650..5123)
                     /locus_tag="VG272_11655"
     CDS             complement(4650..5123)
                     /locus_tag="VG272_11655"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWF14385.1"
                     /translation="MSAVKPIPEKYHNVTPYLYVKGAVAAIEFYTNAFGAKQIFLMPG
                     PSGRIMHAELQFGDSIVMLADEDPARGIMSPHTVGGYSVGLHLYTENVDDVMRKALEN
                     GAKQLHAAKDQIYGDRSGSLLDPFGHMWTVATHIEDVTPEELQKRMVAMNRSASA"
     gene            5478..>6397
                     /locus_tag="VG272_11660"
     CDS             5478..>6397
                     /locus_tag="VG272_11660"
                     /inference="COORDINATES: protein motif:HMM:NF019924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HWF14386.1"
                     /translation="MKILFVASEGLPFSKTGGLADVIEALPKSLVALGHEVSVLLPRY
                     RKTKAVGISASSLTVPKGDVLRFPAITEGGAIHGVYFYFVDDPEYFDREQLYGVAGAD
                     YPDNAERFAEFCGAAIEFCKHVWMPDVIHCHDWQSALVPVLLRTRYAADPAVAELPVV
                     FTVHNMGYHGAFPPAEMKKIGLPEDLFSIDGMEFFGKVNFLKGGLIYSDFLTTVSPKY
                     AEEIKTVQYGQGLDGVVRNRADRLTGILNGADYSAWSPERDKVIASRYSAKDLSGKLA
                     CKKDLLETFGLPKDVLGKPVIGIVSRFASQ"
BASE COUNT         1365 a         1644 c         1883 g         1505 t
ORIGIN      
        1 gctgtcgtgg cgatagccta ccagtctcgc cacaaaattc atttggcgga cttcacctgg
       61 aataaatttg tcgccagtgt cggccaagcc aatatctttc ttctttttct ttgcctcgtt
      121 ggaatttacg gatgctacgc actccgcgcg gttcgctggc agcgcttctg caaatacgtt
      181 ggccccacga attttctgaa cacctatgcc ggaactctga tgggattcgc agcgattttc
      241 gttctgggcc gtgccggcga gcctgtccgg ccgctgctcc tggcccgcaa agaacgcctc
      301 ccggtttcaa gcctgtttgg cattttcttt ctggagcgct tttgcgattt cgcggcggcg
      361 gccgcgttgg cgtgcctctc cctgcttgta tttccaaatc gattgtcaga tgccggcgcg
      421 gacatgggtt gggtggaaag cgcgcggcac ggcgggtggc tgctgctggc cgcgatgacc
      481 gctctgatcg tgatgctcgt ggtataccgg ctgcacggtg cggccatcct cgatcgcatc
      541 ctggcgcgct ggcgcgacgc gggcgggttt cgcagccgct ttgcttcggg tattaccggc
      601 attagcgagg gtttacaggc aattcgaacg acgtcggacc tgttggctgc cattttctac
      661 accgcggctc actgggtgct cgctacgctg atttacctgg gcgtggccaa agcgttcggc
      721 gcttccttca tgcattcgga catgaatttt tcgggtgcga tgcttttgct ggccgtcacg
      781 ctcgtcggtt cggtgttgca gctacccgga gtgggcggcg gcgcgcaaat cgccagtttt
      841 atcggcctga ccaagatctt tggagttgag caggagcctg ccgcagccat ggccgtggtg
      901 ctctggctca tcacgtttgc cgggggaacc ctggccggca ttccactgct gattcatgaa
      961 ggaatgtccg tgggtgaatt gcgccaactg gcgcgagccg aagcggaagc ggaagaatcg
     1021 ggcaagcata tcgcggtcac ggaagacaat ggcgtcagcc cttcttcgcg cgcggcgaaa
     1081 gccaagccgc tggaagagaa gccatagtga aatgtccttt ttgttcggtg atggaagaca
     1141 aggtcattga ttcacgcgag ggccgcgccg gcgacctgat ccgacgccgg cgcgaatgcc
     1201 tgaagtgcgg gcggcgctat accacctacg aacgcatcga cgaaattccc tacatggtca
     1261 tcaagaaaga tggccgccgc gaacgattcg accgccagaa aattttgcag ggattgctga
     1321 aatcatgcga aaagcggccg gtggcgaccg cgaagctgga agcgctggtg gatgaggttg
     1381 aacgattcgt gcacgaagcg cccgagcgcg agcggaagac gagcgaggtc ggcgaacttc
     1441 tgatgaaccg catcaagaag ctggataagg ttgcttacgt gcgattcgct tccgtgtatc
     1501 tggattttaa ggatgtgaag gaattcatgt ctgaattaaa aggtttgtta aaagatcgct
     1561 ccaagaaata gccctgcctc tgcacatttt cgataccagt acttataagt cttctcgcga
     1621 agttcccatt tgatgaatag cggtacattg ctatcgctct ttcccggcgt gggcgacatt
     1681 tccttgcagc gcgcaactat tttttgcgtt gaactgttga cagaggaatc gttgacattt
     1741 ccttggacgg aaggaaacgg atgcgattta gacggctcgc tttagcggct ctcgtggcgt
     1801 tgtcgggcaa tcaagccgcc actcgcacaa aactcgaatt cagatgaggg gctttcgctg
     1861 acatttcaag tgggttccac gcggaagctc gagcaaatta ccgcggattg cgattgggtc
     1921 gcccgggagg cgacaaactc atttttttgt ttggcgatac tttcggaaac acgaaattgg
     1981 ctacacgctc tcaacgtgga atccgtacac cgttgcggaa atggaatcag atttcaaaat
     2041 tacaggtgac agggaagact agtgagaatt cagacaaaaa taaaaacggg cccggaacga
     2101 tccttcccgg cccattttta tttctgcaca gagaaaatct tgcttaaccg attcgcaaat
     2161 tattttccac ggagaagacg ccgggaaccg agtttgcgac aatgcctgcg atgtttttat
     2221 ccatctgatt catgaccacg ccatcgagag acacgtggcc gtttctcaca ataatgtgaa
     2281 cctggggaat cgctcccatg gtgtaacggc ccaagtccgc ctttgaaaag acggtgcgat
     2341 attctgcgcg gcggatctgg tcatcaaact gggacggagg aagaacctcg atgcggttaa
     2401 tcacattggt tacgccggga atggaatcca caaatctttc cgcgtcgtcc ttggtggtcg
     2461 catgttcact gatcacctgg cctttcaata cgacttccgt gccattcacg tcgtactcta
     2521 gattatcgaa tacgccatac catggaagca tggcgagctc atgatggacc tggtcgctaa
     2581 gcgacaacac tttatggctg gtggtggcgg aggctagcac attcggagcg ctccacacca
     2641 ggcccaggct tccaaccaat acgattgcga ggaaatacct tagaagactt tcacggttac
     2701 ccatggaagc tcccctttct gcggcctctg cgggccgtct tgccgttttt cttttcactt
     2761 tgttagatgc atgtattcgg caaaaagagt cctgagcaca ctgtgcatcg aacgttgttc
     2821 gggccaggcc aaccgttctg atacgattaa acgcatcctt agattttcat aatcggggcg
     2881 cggcagagag gaaaatgcaa agcaaatcga tttgggttat tgcgcatcgc ggagcctcgg
     2941 gccacgcacc tgaaaatacg atggcgtcgt tccggcgagc cgtggaattt ggcgcgcgct
     3001 ttatcgaaac cgatctgcag ctcacgcgcg acgcacgcgt gatcgccatt cacgacttga
     3061 catacgatcg gacctcgaca gggaaggggg acgtgcacga tcttccgctc aaacaagtcc
     3121 gcgcgctgga tgcaggttcc tggttcgggg atcgcgaggg aaaatcctac gcggcggaaa
     3181 aagttcccgt gctagaggaa attctggatt ttgcggaaaa gaatgacgtt attttttatc
     3241 tcgaaattaa atccggacaa gcatggggca ttgagcacgt ggtggtggcc actttgcgcg
     3301 atcgcaacgc aagcgcgcgg gtggtgatta tctcgttcga tcccactacg ctagacgccg
     3361 tacaacgcct cgattcgaca atgatgaccg ggctgttatg cgaaattcct tccagcgatc
     3421 tggtggagcg gacggtgcgc gcgggcgcgc ggcagctgat tcttcgcgtg gatctaatca
     3481 catcagcggt taccgaaaaa gcgcatcgcg ccggattgca ggtggtggcg tggacgatta
     3541 atgacgtgga gcagatgcga acgacgatcg cggcgggcgt ggatggaatt attacggact
     3601 atcccgaccg cttgttggag ctgttgcgag agagcgctga gtgaggaact gaacgaaaac
     3661 atttaacagt cgcggcaccg aggatcgaga agaaatgcgg agggagccgc cgtggcctat
     3721 tttgccagtt ggaataggtt tacgaactgg gggatcgtaa gttgctccgc gcgtgcatca
     3781 gagggtaaac ctgctgcggt aattgtttct tccgttctcg ggcccagacc ttggcgcaaa
     3841 ttattccgca aggtttttct tttctgcgcg aagcactctt ttacaaattc cagaaacgcg
     3901 tgctctcccg ggacggggtt gcttacacgt gcgcccggca tgtgaaaact gaccagggcg
     3961 gaatccactt ttggcggcgg gctgaacgct tctggcggga tgcggaattc aatcgacggg
     4021 gttccatacc actgcgatgc gacggaaaga tatccgtagt cgcggcgtcc gggctgcgcc
     4081 acaattcttt cggctacctc cagctgaatc acgatgtgaa tggctgcgat gcgttcggca
     4141 tgttcgaaca ggcgatgcag aatgggcgac gtgatgtaat acggcaaatt tccgtaaacg
     4201 gagaagcgtt cgcctcttgt caatttttca aaatccacgg ccattacgtc gctgtgaaca
     4261 atttctacgt tgtttaaatg cgcggtggat tggcgcaagc ggcgggccag cggcgggtca
     4321 agttctacgg cgaagagctt gctcgcattt tgcgcgagtc gggtggtcat ttcgccgtgg
     4381 cctgcgccga tttcgaccca cacgccactg cccatgcata cggcgtctgc aatctgttcc
     4441 tgccagtctc tatcggcaag aaaatgttgt ccaagcctct cgggtgacat ggaagaagag
     4501 tgtagcaagc gggaagccgg cgcagggtgc agatgtggcc tccgcgactg gatgatcgaa
     4561 ctgctgtagc cagcctgttt agaggcggcg gatttagatt ccagcatagc gtccaccctt
     4621 cctctggaga ggcggactac agcagttgtt tacgcgctgg cggagcggtt catcgccacc
     4681 attcgtttct gcagttcttc gggcgtaacg tcctcgatgt gcgtcgcgac ggtccacata
     4741 tggccgaacg gatcgagcaa agaacctgag cggtctccgt atatttggtc tttcgcagca
     4801 tgaagctgtt tcgctccatt ctccaaggct ttccgcatca cgtcgtccac attttcggta
     4861 tacagatgca ggcccacaga gtaaccaccg accgtgtggg ggctcataat tcctctcgcg
     4921 ggatcttcat ccgcgagcat cacgatcgag tccccaaatt gcaactcggc gtgcatgatt
     4981 ctgccgctcg gtccgggcat taaaaagatc tgcttggctc caaacgcgtt tgtataaaac
     5041 tcgattgccg cgacggcgcc ttttacgtac aagtatggcg tgacgttgtg atatttctca
     5101 ggaatgggtt ttaccgcaga catgtttcac ctcgttagca ctggaatttt tgggcgggat
     5161 ttattgcctg cacggaagca cctttaccgg ctgggagcct ccattccagt ctacatcgaa
     5221 acgcggcagc gggcgacggg cgtaaactga aatattttaa acgtgggcag tagctcggtt
     5281 tcgttttggc atagctggag cgaagcccgc ccggaaggcg gcagccgcga caagtgcccg
     5341 cggttttgta gcgccgttcg gtccggtgat cttcaggacc gaacggacac actgacgacg
     5401 ggacagctcg ctacccgaca gcgagtgtcc ttgacgcgcg gaaaagcttt cagtaggttg
     5461 gaggacctgg atcggctatg aaaatacttt ttgtggcgtc agaggggctt ccattttcaa
     5521 agaccggcgg gctcgcggat gtcatcgagg cgctgccgaa atctctcgtc gcgctgggcc
     5581 acgaagtgag tgtgctgctt ccgcggtacc ggaagacgaa ggccgtgggc atcagcgcat
     5641 cgagcctgac ggtcccgaag ggcgatgttt tgcgctttcc ggcgatcacg gaaggcggcg
     5701 cgatccacgg cgtgtatttt tattttgtgg acgacccgga gtatttcgat cgcgagcaat
     5761 tgtatggcgt cgccggggcc gattacccgg ataacgccga gcgctttgcg gaattctgcg
     5821 gagcggcaat tgaattctgc aagcacgtgt ggatgccgga tgttattcat tgccatgact
     5881 ggcaatcggc gctggtccct gttttgttgc ggacgcgata tgccgctgat cccgcggtgg
     5941 ctgagttgcc ggtagttttc accgttcata acatggggta tcacggggca ttccctcccg
     6001 cggagatgaa gaaaatcggg ttgccggaag atcttttttc gatcgatggc atggaatttt
     6061 ttggcaaagt gaatttcctg aaaggcggcc ttatttattc ggattttctg accaccgtga
     6121 gcccgaaata tgcggaagaa ataaaaacgg tgcagtacgg gcagggcctg gacggggtag
     6181 tgcggaatcg agcggaccgg ttgacaggaa ttctgaatgg cgcggattac tcggcgtgga
     6241 gcccggagcg agacaaggtg atcgcctcgc gctactcggc aaaagatttg tcgggaaagc
     6301 tggcctgcaa aaaagatctg ctggagacat ttggcttgcc gaaggacgtg ctgggcaaac
     6361 cggtcatcgg aatcgtctca cgatttgctt cgcaaaa
//
LOCUS       DASZMB010000250        19865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3548566, whole genome shotgun sequence.
ACCESSION   DASZMB010000250 DASZMB010000000
VERSION     DASZMB010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 19865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19865
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3548566"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VG272_11665"
     CDS             <1..472
                     /locus_tag="VG272_11665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWF14387.1"
                     /translation="PGDGTALYDAIFLGSQGLTKNPAGRRRAIVLVTDAGETTSRADF
                     ETARRAALRADALLYTIVVRAVKTEGGRNTAGEHALETIADSTGGAIYFPDSIAQLGD
                     MFDRIDRELRTQYRIAYYPTPRPPAGTYRNLEVRATCDCMVRSKKSYYSGGRLD"
     gene            587..1390
                     /locus_tag="VG272_11670"
     CDS             587..1390
                     /locus_tag="VG272_11670"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HWF14388.1"
                     /translation="MNSFLDVAIETAKEAGSILLEEFDRPVKISYKGEVDIVTQADKR
                     SEQAIVSRLRNHFPKHAIVAEEGGGSESGSPFRWHVDPLDGTTNFAHGYPCFAVSLGL
                     EEAGEPIVGVVYQPVSGELFTAVKGGGAYLNQKKIEVSRIDTLSTSLLCTGFPSVKRS
                     QNPNIHYYWDFTLRSHGVRRDGSAAMDLAAVACGRFEGFWEFGLHTWDTAAGILLVRE
                     AGGRVTQFNGQPYRVGDRELLASNSRVHSEMQDVAASIAEKASTKSLPQ"
     gene            1540..2460
                     /locus_tag="VG272_11675"
     CDS             1540..2460
                     /locus_tag="VG272_11675"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWF14389.1"
                     /translation="MMGNTVIFPVPGSPSAGDKLYRWLGRWRQGGIEQYSAESAQKPL
                     PVWRSGERFFVNEERIWLDTLPNAFHGLRIVQISDIHHGLFLPKEWLSDAVRKANRLN
                     PDIIALTGDYVTYSRRNIEPAAEILGRLRARYGVYAVLGNHDFRVDADAITSALRRQH
                     IDVLRNRHVSLQFGGESVYLAGVDDYGYGADLRRAMQGIPKEAARVLLAHNPRIIRLA
                     SRHDVSLVLSGHTHGGQVNLPVLGTVYGRSPERLRYKMGWDRMGATQIYVSRGIGTIV
                     LPCRLRCPAEITHLELLQGASGSSQIIAAD"
     gene            2473..3783
                     /locus_tag="VG272_11680"
     CDS             2473..3783
                     /locus_tag="VG272_11680"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HWF14390.1"
                     /translation="MRAVDLIRRKRDSGELTPEEIHQLIAAYTRGDIPDYQMSAWLMA
                     VVLRGMNRAETTALTEAMLQSGRILDFSDIAGCKVDKHSTGGVGDKTSLIIAPIVAAG
                     GLKVPMISGRGLGHSGGTLDKLESIPGFNVNLSLVEFRRVLETCGCSLIGQTAEIAPA
                     DKKMYALRDVTGTVENASLICASIMSKKLAEGIDALVLDVKTGSGAFMKKHEDAVNLA
                     ELMVETGKRMGKKVTALLTDMNQPLGRMAGNAMEIAESIEVLAGGGPPDLRELCLDLA
                     AWMFLLGERVKTVDEGKKLSSDLIATGKAREKFREIVAMQGGDTAVVDDPRRLVRARQ
                     TLDIVSSTAGYVAAIQCEQLGTAGVVLGGGREKKEDKIDPAVGLVFHKRIGDGVQHGE
                     PLCTLHYNSDERLSEAKRLAESAYRIETVKPQVLPSLIYQVIGA"
     gene            4146..5381
                     /locus_tag="VG272_11685"
     CDS             4146..5381
                     /locus_tag="VG272_11685"
                     /inference="COORDINATES: protein motif:HMM:NF019285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HWF14391.1"
                     /translation="MGRFTGVLGLLTMLGLAYVFSTNRRAIKLKMIVWGLALQIAFAF
                     LAFYWTLGQKIFFRAGDAVNRLLAYAFVGSEFLFGELGKQHSSIGFIFAFQVLPTIIF
                     IASLFALLYYLGVMQVVIRAAAWVMQRVMGASGAESLNVAASIFMGQTEAPLTIRPFL
                     PDLTQSELMTIMTSGMAHVSGGIMAAYIAFGVEARHLLTAVIMTAPGTLLISKMLVPE
                     TEQPKTMGVVHMENVEHDSNFLAAIARGTGEGLAMAVNVGAMLVAFLALIALTNGMMG
                     GIHNFLGGHGIQWFPTSLQQIFGWAFAPVAWIIGVPWHDCIQIGDLLGTRMVLNELVA
                     FSFLGPMKGSLDPRSFTIATFALCGFANFSSIGMQIGGIGALAPNQKDQLAKFGIRAM
                     LAGTMANLMSASIVGIFLK"
     gene            5423..6244
                     /locus_tag="VG272_11690"
     CDS             5423..6244
                     /locus_tag="VG272_11690"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HWF14392.1"
                     /translation="MRADVAAKYILTKTKLRPRIGIVLGSGLGAFANEIAGATGIEYR
                     NIPNFPRSTAIGHAGRMVIGKVTGVPVAVMQGRVHMYEGYSPQEVIFPMRVMARMGIR
                     AVLLTNAAGGINTSFSQGCLVVLRDHINLQGTNPLIGPNDERFGERFPDMTQVYYKPY
                     QAAALEQGKRLGMEITQGVYAALTGPSYETPAEIRYLRTIGADLIGMSTVPEVIAAAH
                     LGIRVLGISCVTNMAAGILDQPITTEEVMETGERVKADFVALLRAVIPRMNKDLP"
     gene            6241..6645
                     /gene="cdd"
                     /locus_tag="VG272_11695"
     CDS             6241..6645
                     /gene="cdd"
                     /locus_tag="VG272_11695"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HWF14393.1"
                     /translation="MSESGAPHDSLIVAARVAREHAHAPYSNFRVGAAIRAKSGRIFT
                     GCNVENATYGLTLCAERVAIFKAISEGERGFDAVAVVADTDNLTPPCGSCRQILWEFC
                     GDAEVILSNLSGKIERYRMSALFPKPFDQSFL"
     gene            complement(6883..7659)
                     /locus_tag="VG272_11700"
     CDS             complement(6883..7659)
                     /locus_tag="VG272_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14394.1"
                     /translation="MQNVLTAIAIPNAAPEMFAYCAAEFSRRPGVADALAQNSTCPIE
                     VLRPVIRYLSVTGVQDLFEDLDRLSTSPYLVEELIASSVINADQRGQLLDLQRTDYEP
                     VSAFAEAAEEAEPDSGKRETMLQKLSRLRVVERVQLALKGGRDERVLLIRDPCKVVQR
                     AVLQSPQLSEQEVEGFSKMTTLSEETLRLIANNRKFRKNYVILRALIFNPKSPLEVTL
                     HLLPMIKATDVKLLSTSKNIADTLRTAAARLMRQRTAQRD"
     gene            complement(7838..8782)
                     /locus_tag="VG272_11705"
     CDS             complement(7838..8782)
                     /locus_tag="VG272_11705"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HWF14395.1"
                     /translation="MIRNSARQMIDTPAPAAYIRWDYWIAQEFMDKGTFGESLKRERE
                     MRGVTLEEISSATRIAERFLRAIENDQWDQLPGGVFNRGFVRAMARYLGLDEESIVAE
                     YTLAVGDRPSVPVWTGSPPTVTPDQPWMAWGVTLLIIIALLVGSWFGVRRFFAWRAAK
                     HATQPPALNNGPSPAIKHDSQEIPPELATPSTTLNHGATTSTGTSGAVPAAPDTASST
                     TPVRAVADRFELKVEAAKKTKITVTADKNVVYQGTIKAGENQFFSAADHFQVSAKDAG
                     ALHLELNGKPLPPIGPSGHSAKITLTRDSLKEIPGGGN"
     gene            9099..10190
                     /locus_tag="VG272_11710"
     CDS             9099..10190
                     /locus_tag="VG272_11710"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HWF14396.1"
                     /translation="MTDNFSGTERRHRVTARGEQREAGEIAVFHELGKALTSSLQLDQ
                     VLRTIMEKIDEFLRPDTWSLLLVDEAKQELYFELAVGKGAQALKDVRIKMGQGIAGWV
                     AQNDEAVIVADVSKDTRFFSQIDEKTKMETRSIVAVPVRYRDHCLGVIELINCVERDG
                     FMEHDMALLEALADFAAIALENARHVKRIHELTITDDCTGLFNARHMDFILETEIYRS
                     QRYGYEFSVVFIDLDHFKKVNDTYGHLVGSKLLADVGQIVKSACRRIDFAFRYGGDEF
                     VIVLPQALKENAFIVARRLHKLIGEKHWLIEQGLDIQFTASIGVASFPSDAKTKLELL
                     HLADEAMYAIKNTTRNGVAAAKIGVIPSK"
     gene            10354..10653
                     /locus_tag="VG272_11715"
     CDS             10354..10653
                     /locus_tag="VG272_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HWF14397.1"
                     /translation="MSNEALGMIETKGLVASIEAADAMVKAANVILIGKEYIGSGYVT
                     VMVRGDVGAVKAATDAGAAAARRVGELVSVHVIPRPHEHVEKILPGGGAPSAIKK"
     gene            10826..12892
                     /locus_tag="VG272_11720"
     CDS             10826..12892
                     /locus_tag="VG272_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWF14398.1"
                     /translation="MSLRDRDLISIQEARELAARAAAAQKKFAEFSQEQVDAIVEACA
                     KAAAENVEPLARAAVEETGFGNVSDKIIKNTLAAVNVPRAIRGMRTVGILREDREKGI
                     VEVASPVGVVAAVIPSTNPTSTAIYKTLIAIKARNAIVLSPHPSAIKCICRAATVLER
                     AALTAGAPEGLIQCMQHTTLEGTRELMRRPEVGVILATGGTGLVRAAYGSGKPAYGVG
                     PGNVPAFIERTADVRKAVADIIAGKTFDYGTICSSEQAIVAEETVRESALEECRKQGA
                     YFLSPQEIEKISRLVFVQGSQTPNTEIVGRAATVIAEMAGIHAPPATRVLIARLGPEQ
                     VGREFPLSAEKLSPILAFYAVENFAAGIALCRRLLEFGGLGHTCSIHSQNRAAVLEFG
                     AAMPAFRVVVNSASVHGSIGYSTNLFPAMTLGCGAPGGNITSDNIGPQHLMNVKRIAW
                     EARAVEHRTVPADQRMAGAANSHEPVAVQAEVNVSTVPESIQAAAPGAPVVSQEAKSE
                     EKKIAVPAAAASTGIDRVAIARVVENVLAAQGIARGSGVPQSSPVSAAMPDATASETA
                     PKEKELDSPASIAAEIANRFFGATANAPQNSGKTAPSGEAQALSQCPPEGGRYMLPDA
                     GKKVEHPAKAAIEIQVFVSENDVRRAMTRSEKIYIGRKTILTPSARDLGMEHEVFVET
                     ETGSIR"
     gene            13142..13294
                     /locus_tag="VG272_11725"
     CDS             13142..13294
                     /locus_tag="VG272_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14399.1"
                     /translation="MLEPQGLKPNLFCSIYVVAKATTHKDFEVLTQTLKLVLLYDLTV
                     GFHLTA"
     gene            13309..14070
                     /locus_tag="VG272_11730"
     CDS             13309..14070
                     /locus_tag="VG272_11730"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWF14400.1"
                     /translation="MRKISVAVISLACVLLYSRSAVAQTSEVHVLASNGIKAVLEDLR
                     AQGEHAIGHPITVDYKPTANLKQDIDAGAQFDVVIVTVESIAQLSKEGKTLGGEGTPI
                     SRVGVGIGIRKGAPRPDISTPEALKRALLGTKAIAYGPVGASLPYITHMLESLGIAEA
                     MKPKTVLLKTSDETNEAVADGKVDFGMTLVSEILPAKGVELLGPFPEEFQGYVRFNAA
                     VSANAKNPEAANALVKFLAGPAAAPFIKARGMQAH"
     gene            14413..15204
                     /locus_tag="VG272_11735"
     CDS             14413..15204
                     /locus_tag="VG272_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14401.1"
                     /translation="MRSRLLGPAVALAVTLACSFSASAQTEKPSDTPAASGPFDPHDL
                     SGVWSQEHPRNLPVVERYWNYEFNKEEPPMTAWGQMQFNAAKSSFGQHTYPLAETNDP
                     LYHDCTPPGLPRVYLHPFPMEIVQMPGEILILFEYDSLRHPIYTDGRKHDEALGPLWM
                     GDAIGHWEGDTLVVDTTNFNDKTWIDRVGHPHTADLHLVEHIKRTDRTHLADDITVDD
                     PKAYTKPWTAHLDFILRPTWTLGEQFCEDEHSFENFDKKGTTSAK"
     gene            15412..15789
                     /locus_tag="VG272_11740"
     CDS             15412..15789
                     /locus_tag="VG272_11740"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HWF14402.1"
                     /translation="MRKLALFAVLACAALFVSAPLPAHHGEANYDTDKIVSVKGTVTE
                     FQFINPHTLIALDVKNDKGEMEKWNCEARSPGMLVRAGGWDKATLKVGDVITVYGYRG
                     KSGALVLRLQKLTLADGTVKDNL"
     gene            16086..16883
                     /locus_tag="VG272_11745"
     CDS             16086..16883
                     /locus_tag="VG272_11745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14403.1"
                     /translation="MRNQPLGFAVALAIALSISPTLSAQSKKAAEAPAADKGSFDPHD
                     LSGVWMQDHPRLITVDARYWVYKFTLEEPPMSDWGETKFKATKTSFGPHAFPLAETND
                     PVYHTCDPLGFPRIFLYPLPLQIVQTPTEVIMLFEYDWLRHQIFTDGRKHDESLGPLW
                     MGDAIGHWEGDTFVADTTNFNDKTWLDRMGHPHSDVLHVVERIRRVDHDHLVDDMTID
                     DPKAYTKPWTAHQIFALQPKWTIAESFCEDEQSFESIDESGAAPVKK"
     gene            16938..17741
                     /locus_tag="VG272_11750"
     CDS             16938..17741
                     /locus_tag="VG272_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14404.1"
                     /translation="MRKCLIGLVMAAFVISGSSRSASAQTKQPSQSPSTAGSSSSSFD
                     PHDLSGVWMQDRPRMGRVIERYWIYELTLEEPPMTAWGLAQYKAAKSSFGNHSFPIAE
                     TNDPIYHSCSPTGFPRAFFHAFPIQIVQSPTEVVVLFEWDSLRHQIFIDGRKHDESLG
                     PLWMGDAIGHWEGDTLVADTVNFNEKTWLDRVGHPHSDALHVIEHIRRVDHDHLEDEI
                     TIDDPKAYSKPWTGKIIFALKPNWTLAEEFCEDIPSFDAIEKQETTPAK"
     gene            17864..18241
                     /locus_tag="VG272_11755"
     CDS             17864..18241
                     /locus_tag="VG272_11755"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HWF14405.1"
                     /translation="MKKLALVLSMSFVFLLISAPIFAHHGETNYDTEKLVTVKATVTS
                     FEFINPHTQITLETKNEKGEVEKWTCEARSPAMLVRNGDWSKNTLKPGDMITAIGFRA
                     KNGANILRLKKIVLSDGKELPDL"
     gene            18383..18778
                     /locus_tag="VG272_11760"
     CDS             18383..18778
                     /locus_tag="VG272_11760"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HWF14406.1"
                     /translation="MNLRPAALFFLLAGLLLVSAPVFAHHGNSAYDEAHWITISGTVT
                     EFVWSNPHCQIFVDVKGDKGKVVNWAIESQSPGIMRRNNWTRNSLQPGDQITVTLAPA
                     KNGAAVGFSGNADGKVVFADGRVLKMNNR"
     gene            18872..19678
                     /locus_tag="VG272_11765"
     CDS             18872..19678
                     /locus_tag="VG272_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14407.1"
                     /translation="MRSRPPVWIACSMAALTAAFAISTIVHAQSNLPQGTPKSAAAPA
                     PAPKRDLTGVWQLQGGNGGAQPIAEDKDMPPMTPWAKVKFDAQKPGYGSRGAAGGNDP
                     ILQCDPIGFPRIMLMPTPNELLQAPGRTIQFWEREHEWRTIWTDGRPLPTDPDPAWYG
                     YAIGHWESDDTFVVESMGFNDKTWLGPTGYPHSEEMRVVERYRRVDHDTIAYDMTITD
                     PKAYAKPVVTPRRVMKLKPNDEISEQPCVWSQENEFAQRIRNPATPKSAK"
BASE COUNT         4481 a         5122 c         5748 g         4514 t
ORIGIN      
        1 ccccggagac ggaacagccc tgtatgacgc tatattcctg gggtctcagg gactgacgaa
       61 gaatccggcg ggtcgacgca gggccattgt tctggtcacg gatgcgggtg agacgaccag
      121 ccgcgcggat tttgagacgg caaggcgcgc ggcgcttcgt gcggacgcgc tcctctatac
      181 gatcgtcgtg cgtgcggtaa agaccgaagg cgggcggaac actgccggtg aacatgctct
      241 cgaaacgatt gcagactcca caggcggagc catctatttc ccggactcca ttgcccaact
      301 tggcgatatg ttcgatcgca ttgaccgcga gctgcgcacg caataccgca tcgcgtacta
      361 tcccactccc cggccgcccg caggcacgta tcgtaacctc gaagtgcgcg cgacgtgcga
      421 ttgcatggtc cgcagcaaga agtcttatta ttcgggcggg cgcctggatt aagaattccg
      481 tgagttgtga accgtaattc gtggaaaccc cggtattctt acctatttaa cctgtgcgga
      541 atctggcatc atttattatg agagtattgc gaggcctcat acgcgaatga attcatttct
      601 ggatgtggcg attgaaacgg caaaggaagc gggatccatc ctgctggagg aattcgatcg
      661 cccggtgaag atcagctaca agggtgaagt agatatcgta acgcaggccg acaagcgttc
      721 tgagcaggcc atcgtttccc gcttgcggaa ccatttcccg aaacacgcca ttgtggccga
      781 agagggcgga gggtcggaaa gcggatcgcc ttttcgctgg catgtcgacc cactggacgg
      841 cacgactaat tttgcgcacg gctacccgtg ctttgctgtg tcgctggggc tggaagaggc
      901 gggcgagccg attgtgggcg tcgtctacca gccggtaagc ggagaactct ttacagcagt
      961 aaaaggggga ggggcttacc tgaaccaaaa gaaaatcgag gtttcgcgaa tcgatacgct
     1021 gtcgaccagt ttgctgtgca cgggctttcc cagcgtgaaa cgctcgcaga atcccaatat
     1081 tcattactac tgggatttca ctttgcgttc ccatggcgtg cggcgagacg gttcggccgc
     1141 tatggacctt gccgctgtgg cctgcggacg atttgagggc ttctgggagt tcgggctgca
     1201 tacctgggat actgcggcgg gcatcttatt agtaagggag gcaggtggca gagtcaccca
     1261 gttcaacggc cagccctata gggtggggga cagagaatta ctagcgtcga acagtcgcgt
     1321 gcattccgaa atgcaggatg tagccgcgag catcgcggag aaggcttcga caaagtccct
     1381 tccgcagtaa cccggatgta accgtcaggg aagcttcaga agaggtcaat tcaacatttt
     1441 cattttgcac acgcacccgt aaatcattgg attgacgcat gttcccccga atgataggct
     1501 cgaaatagaa gccatattta ctgaggtatt tactgaggga tgatgggcaa taccgttatt
     1561 ttcccggttc ctggaagccc cagcgccgga gacaagctat atcgttggtt aggtcgatgg
     1621 cgccaaggcg gcattgagca gtactctgcg gagtctgcgc agaagccgct gcccgtttgg
     1681 cgctccggcg aacgtttttt tgtgaatgaa gagcgcatct ggctggatac tcttcctaac
     1741 gccttccacg ggctgcgcat cgtacaaatt tctgacattc atcatggcct ttttcttccc
     1801 aaggaatggc tttccgacgc tgtgcggaag gcaaaccgcc taaaccccga catcatcgct
     1861 cttacaggag actacgttac atactcccgg cgaaatatcg agccggcagc ggaaatcctt
     1921 ggccgcctgc gagcgcgtta tggagtttat gcggtccttg gcaatcatga cttccgcgtg
     1981 gacgcagacg cgattacatc cgccctgcga cgccagcaca tcgacgttct gcgcaaccga
     2041 catgtttcct tgcagtttgg cggagagtct gtttacctgg ctggcgtgga cgattacgga
     2101 tatggtgcgg atttgcgccg cgcgatgcag ggcattccga aagaggctgc ccgcgttttg
     2161 ctggcgcaca atccaaggat tattcggctc gcttcccgcc acgatgtgag cctggttctg
     2221 tccggccaca cgcacggtgg gcaggtgaat ttgcctgtgc ttggcaccgt gtacggccgg
     2281 tcgcctgagc ggcttcgcta taaaatgggc tgggaccgca tgggagccac ccagatttac
     2341 gtaagccggg gcattgggac catcgttctg ccttgcagac tgcgctgccc ggccgagatc
     2401 acccatttag agttgctgca aggcgcttct ggctcttccc aaataatagc agcggattaa
     2461 catcccaaac gaatgcgcgc cgttgacctg attcgacgca agcgtgactc gggagaactc
     2521 actcccgaag aaatccacca actgatcgca gcctatacgc gcggcgacat ccccgattac
     2581 cagatgtctg cctggctgat ggccgtagtg cttcgcggga tgaaccgggc tgaaacaacc
     2641 gcgctcacgg aagccatgct gcagtcgggg cgcattctgg atttttctga catcgctggt
     2701 tgcaaggtgg ataagcactc aaccggcggc gtgggagaca agacttcact gatcatcgcg
     2761 cccatcgtgg cggctggcgg attgaaggtg cccatgatca gcggacgcgg attgggtcac
     2821 agcggcggca cgctcgataa gctggaatcc attcccggtt ttaatgtaaa tctgtcgctg
     2881 gtcgaatttc gccgcgttct ggagacttgc ggctgctcgc tcattgggca gacggctgaa
     2941 attgctcccg ccgacaaaaa gatgtatgca ttgcgcgacg ttacggggac cgtagaaaac
     3001 gcgtctctga tctgtgcttc gatcatgagt aagaaacttg ccgagggaat cgatgcgctg
     3061 gtgctcgatg tgaaaaccgg ttccggcgca ttcatgaaaa agcatgagga tgccgtaaac
     3121 ctcgccgaac tgatggttga aaccgggaag cggatgggca agaaagttac ggctctctta
     3181 acggacatga atcagcctct cggacgcatg gcgggcaatg cgatggaaat cgctgagtca
     3241 attgaagtgc ttgccggagg cggtccgccg gacctacgcg agctatgtct ggatctcgcg
     3301 gcgtggatgt tcctgcttgg cgagcgcgtg aagactgtgg acgaggggaa gaagctatcg
     3361 tcggacctga ttgccacggg caaggcgcgc gaaaaattcc gcgaaattgt tgcaatgcag
     3421 ggcggggaca cggcggtcgt ggacgatccg cgacgtctgg tacgcgcgcg gcagacattg
     3481 gatattgttt cctcaacagc ggggtacgtt gcggcaatcc agtgcgaaca gcttggtacc
     3541 gctggtgtag tgctgggcgg cggccgcgaa aagaaagaag acaagatcga tccagctgtc
     3601 ggtcttgtgt ttcacaaacg tattggcgat ggcgtgcagc acggcgagcc gctgtgcacg
     3661 cttcactaca attcagacga acgtctctcc gaagcgaaac gtttggctga aagcgcatac
     3721 cgcattgaga ccgtgaaacc gcaagttctc ccttcgctca tctatcaagt gattggcgct
     3781 tagaaggtcc gcattttata ttgaggacgt agtaacaagc gaaaattatg ggtcggctgt
     3841 aggggcacgg caatgccgtg cccctacaac tgcaatttcg ccaacatttg accatttatt
     3901 gaaatcggtg gcttcggaag tgtcccaaaa cgttctcact ccgtgggcaa gctgcgcttg
     3961 gccaaaaatt ataggccgac agactgaagg tgagtatttc ctcttatttg ccagcccttc
     4021 tgtaatcctc atattggaac caatattcct gaacatccaa aattccacat atcgactctc
     4081 cggcaagcgt gctaatgtga cgggcatttc ccgtgggggg catgcgggat gcgaggtgag
     4141 gacgaatggg ccgattcacg ggcgtgcttg gattgctgac aatgcttgga ttggcttacg
     4201 tgttttcgac caaccgtcgc gccatcaaat tgaaaatgat cgtatgggga ttggctctgc
     4261 agattgcttt cgcatttctg gctttttact ggacattggg gcagaagatt ttttttcgcg
     4321 ccggcgatgc cgtgaatcgc ttgcttgcct acgcatttgt cggttctgaa tttttgtttg
     4381 gagaattggg caagcagcat tcctcgattg gatttatttt cgcgtttcag gttttgccga
     4441 ccatcatttt tatcgcgtcg cttttcgcgc tgctgtatta cctcggcgtg atgcaggtgg
     4501 tgattcgcgc ggcggcctgg gtgatgcagc gcgtgatggg cgcgagcggg gcggaatcgc
     4561 tcaacgtcgc cgccagtatt ttcatggggc agacggaggc gccgctgacg attcggccat
     4621 tcctgccgga cttgacccaa tcggaattga tgaccatcat gaccagcggg atggcgcacg
     4681 tttccggcgg catcatggcg gcgtacatcg catttggagt ggaagcgcgg cacttgctca
     4741 ccgcggtgat tatgacggcg ccgggcacgc tgctcatttc caagatgctg gttccggaaa
     4801 ccgagcagcc aaaaaccatg ggcgtcgtgc acatggaaaa tgtggagcat gactcgaatt
     4861 ttctagcggc cattgcgcgc ggcacgggag aagggttggc gatggcggtg aacgtgggag
     4921 ccatgctcgt cgcgttcctg gcgctgatcg cgctgaccaa cggaatgatg ggcgggatcc
     4981 acaattttct gggcgggcac ggcattcagt ggtttcccac gagcctgcaa caaatttttg
     5041 gctgggcgtt cgctccggtt gcctggatta ttggcgtgcc gtggcacgat tgcatacaga
     5101 tcggagatct gctgggcacg cgaatggttc tcaacgaact ggttgccttt tcctttctcg
     5161 gcccaatgaa gggctcgctc gatccgcgtt cgtttacgat tgcgacgttc gcgctatgcg
     5221 gcttcgcaaa tttcagttcg attggaatgc agatcggcgg cattggggcg ctggcgccga
     5281 atcagaaaga ccaacttgcg aaatttggaa ttcgcgccat gctggcaggg acgatggcga
     5341 acctgatgtc cgcgtccatt gtagggatat ttttgaaatg agcgcatccg gaccagtgaa
     5401 gtcaagaccg acccggagtt ttttgcgtgc tgacgtcgcc gcaaaatata ttctgacgaa
     5461 aacgaaattg cgtccgcgca ttggcattgt gctgggcagc ggtttaggcg catttgcgaa
     5521 cgagattgcg ggcgcgacgg gaatcgagta ccggaacatt ccgaatttcc cgcgctcgac
     5581 cgccatcggc catgccgggc gcatggtgat cggcaaagtt accggcgtgc ccgtggccgt
     5641 gatgcagggc cgcgtgcaca tgtacgaggg ttactcgccg caagaagtga ttttccctat
     5701 gcgcgtgatg gcacgcatgg gcatccgcgc ggtgttgctg accaacgcgg cgggcggcat
     5761 caatacaagt ttcagccagg gatgcctcgt ggtgctgcgc gaccacatca atctgcaggg
     5821 gacgaatccc ctgatcgggc cgaatgatga gcgatttggc gagcgctttc ctgacatgac
     5881 gcaggtgtat tacaaaccct atcaggccgc ggcgctggaa cagggaaaac gcctggggat
     5941 ggaaattacg cagggagttt acgcggctct gaccgggcca agttacgaaa cacccgctga
     6001 aattcgctac ttgcgcacga ttggcgcgga cctcattggt atgtcgaccg tgccggaagt
     6061 gattgccgcg gcgcacctcg gaattcgcgt gctgggaatt tcctgcgtca cgaatatggc
     6121 cgccggaatt ctcgatcagc cgattaccac cgaggaagtg atggaaacag gcgagcgggt
     6181 aaaggcggat tttgtggcgt tgcttcgcgc cgtgattcca cgcatgaaca aggatttgcc
     6241 atgagtgaat ccggcgcgcc ccatgattcg ttgatcgtag cggcgcgagt cgctcgcgaa
     6301 cacgcccatg cgccgtattc caactttcga gtgggtgcgg cgatccgcgc gaagtctggg
     6361 agaattttca caggctgcaa cgtggaaaat gccacgtatg gattgacgct gtgcgccgag
     6421 cgcgtggcga tttttaaggc gatctccgaa ggcgagcgcg gatttgacgc cgtcgccgtc
     6481 gtcgcggata cagacaacct tacgcctccg tgcggatcgt gccgccaaat tttgtgggaa
     6541 ttttgcggcg acgccgaagt aattctttct aatttgtcgg gcaaaattga gcgttaccgg
     6601 atgtccgcgc tatttccaaa gccgttcgat caatcgttcc tgtagggtcg tgattcgtga
     6661 tttgtgaatt gtcattcgtg aagttcccgg tacatcttgc aacattctca aatctcaaat
     6721 ctcaaatctc atccattctc catttgccgg gatttgtgag ggcaatctgg gcacgtcaaa
     6781 tccaaaggat gagtccaaac tagagagccg catttaacga atcgcaactc atggagggta
     6841 gttcacgaat cacaaatcac aattcacgaa tcacgtgctt tcttaatcac gctgtgcggt
     6901 tcgctggcgc atcaggcgcg cagcggctgt gcgcagcgta tcggcaatat ttttgctggt
     6961 ggaaagaagt ttgacgtcgg ttgccttgat cattgggaga aggtgaagcg tgacttcgag
     7021 cggggattta ggattgaaaa tcagggcgcg caggatgaca taattttttc ggaatttgcg
     7081 attgttggcg atgaggcgaa gagtttcctc gctgagagtc gtcatcttcg aaaaaccttc
     7141 cacctcctgc tctgacaatt gtggagattg caggacggcg cgctgcacaa ctttgcacgg
     7201 atcgcggatg agcagtacgc gctcgtcgcg gcctcccttg agcgcgagtt gcacgcgctc
     7261 taccacgcgc agccgcgaca atttttgcaa catcgtttcg cgctttccac tgtccggttc
     7321 cgcttcttcc gccgcttccg caaatgcgct gacgggttcg taatcggtcc gctgcaaatc
     7381 cagcaactgg ccgcgctgat cggcgttaat cacggaggat gcgatcaatt cctcaaccag
     7441 gtacggcgaa gtgcttagcc ggtcaaggtc ttcaaacaag tcctgaacgc ccgtaaccga
     7501 gagatagcgg ataacaggcc gaagcacttc gatggggcat gtggaatttt gcgccagtgc
     7561 gtcagccacg ccgggccggc gtgaaaattc cgcggcgcaa tacgcgaaca tttcaggcgc
     7621 tgcgttgggg atggcaatgg ccgttaagac attttgcaat gtctgcgtca gcaggatgtc
     7681 cgctgcttta tcgcgaaccg cggaatcgga atcggcagta aggatacaga gggtttcgat
     7741 gcggtcgttc ggctcaaatt cagccgcttc gttacatatt gccagcttac gttccagagt
     7801 agcgcgtccc gaacgaattt catcgcgaag ggcttcgtta atttccgcct ccgggtatct
     7861 cttttaagga atcgcgcgta agggtgatct tggctgaatg cccggatgga ccgatgggag
     7921 gaagcggttt tccattcagt tcgagatgca aggcccctgc gtcttttgcg gatacttgaa
     7981 aatgatcggc agcggaaaag aattggtttt ccccggcctt gatggttccc tgatagacga
     8041 cgtttttatc cgctgtaacg gtgatcttgg tttttttcgc cgcttcgacc tttaattcga
     8101 atcggtctgc aacggctcta actggcgtag tgctcgaagc agtatccggt gccgctggta
     8161 ccgcacctga agttccagtc gaagttgttg ctccatggtt gagagtcgtg ctcggagtgg
     8221 cgagctctgg cggaatctcc tgcgagtcgt gtttaatcgc tggagatggg ccgttattga
     8281 gcgccggcgg ctgcgtcgcg tgcttcgcgg cgcgccaggc aaagaaacgc cgcaccccaa
     8341 accaactccc tacaagcagg gcaatgatga ttaatagggt gactccccaa gccatccacg
     8401 gctgatcggg agtaacggtc ggagggcttc cggtccacac gggaacgctc gggcggtcgc
     8461 cgaccgcaag cgtgtattcc gcgacgatgc tttcttcatc gaggccaagg tagcgggcca
     8521 tggcccgcac aaacccacga ttgaagaccc cgccgggaag ttgatcccat tggtcgtttt
     8581 cgatcgctct caagaagcgt tccgcgatgc gcgtagcgga ggaaatttcc tcgagggtca
     8641 caccgcgcat ttcccgttca cgttttaggc tttcgccgaa agtaccttta tccataaatt
     8701 cttgagcgat ccaatagtcc cagcgaatat aggcggcagg cgcaggtgtg tcaatcatct
     8761 gccgcgcaga gttgcgaatc aatcagttcg caaattgata gagcgattga ctttccgagc
     8821 atttggatga cgtttctgga attccgttct gaatttgcgc ttccggaaat ttttgtaatg
     8881 cgtgaaaaaa ctggggaaat acaccacggt tcgacagaac caggcaggat ttgccccagg
     8941 attgcttggc cggaggggct tcactttcga gcaacaccct ctataagtgg acgttgcaca
     9001 gatcgacaag agctgactat aatttctagt gcctcaacac cttccgcatg ccagggggtg
     9061 acccacgata gattctggca ccaaggcttt tagtgacaat gactgacaat ttttccggta
     9121 ccgaacgtcg ccacagggtt acggctcggg gagagcaacg cgaggcaggc gagatcgcgg
     9181 tcttccatga gctgggcaag gcgctaacgt cgtccctgca gctcgatcag gttctccgca
     9241 cgattatgga gaagattgac gaatttctgc gcccggacac atggtcgctc cttctcgtcg
     9301 atgaagccaa gcaggaattg tattttgaac tggcggtggg caagggtgcg caggcgttga
     9361 aggatgtgcg catcaagatg ggacagggaa tcgcgggatg ggtggcgcag aacgatgaag
     9421 ccgtgattgt ggctgacgtc agcaaagaca cccggttttt ttcgcagatc gacgaaaaaa
     9481 ccaagatgga gacgcgctcg attgtcgccg taccggtgcg ctatcgcgat cattgtctcg
     9541 gcgtcatcga gttgattaat tgcgtggagc gagacggctt catggagcat gacatggcgc
     9601 tgctggaggc gctggcggat ttcgcggcca ttgctctcga aaatgcgcgc cacgtgaaac
     9661 gcatccatga gttgacgatc acggacgatt gcacgggcct gtttaacgcg cggcacatgg
     9721 attttattct ggaaaccgaa atttaccgct cgcagcgata cggctatgaa ttttccgtgg
     9781 tctttatcga tctcgatcat ttcaaaaaag tgaacgatac gtacggacac ctggttggat
     9841 caaagcttct ggccgatgtg gggcaaatcg tgaagagcgc ctgccgccga atcgattttg
     9901 cgtttcgcta tggcggcgat gaatttgtga tcgtcctccc acaggccttg aaggaaaatg
     9961 cctttatcgt tgcgcggcgc ttgcataaat taattgggga aaagcactgg ttgatcgagc
    10021 aggggctcga cattcaattt accgcgagta tcggcgtcgc atcttttccg tcggatgcaa
    10081 agactaaact agagctgctt catctggcgg acgaagcgat gtacgcgatc aagaacacca
    10141 cacgcaatgg cgtggccgct gcaaagattg gcgtcattcc gtcgaagtaa gctcgcaaat
    10201 ttccttgcaa gcagtttgat ttccacagaa acacccagac cttccaccgc aactccttga
    10261 ttcactacct atgccatgct agtctcaccc cttccggtag cacgccgcag tgtaaatgga
    10321 atggatgcgc cggagagcca agatgaggag cgcatgtcca acgaagcatt ggggatgatt
    10381 gaaacgaaag gcctggttgc ttcgatcgaa gcggcggacg ccatggtgaa ggccgccaat
    10441 gttatcctca tcgggaaaga atatattggc tctggatatg tgacggtgat ggtgcgcgga
    10501 gatgttggcg ccgtaaaagc ggcgaccgat gcgggcgcgg ctgctgcgcg gcgcgtaggg
    10561 gaacttgttt cagtgcacgt gattccgcgg ccgcacgagc atgtggaaaa gattttgccc
    10621 ggcggcggcg cgccttcggc gatcaagaag taacgcgcgc aacgtctgag cggtttgcgt
    10681 tcttgagatt ttttagcccg cctcttcaga ggcggggatt cttgctgcgt acgatcacct
    10741 aagccctcgc ctctgatccc tcgttttcac tcgggacaag cgaggcgagc tacagcacac
    10801 gtccccgagg agaaggtaaa gcgttttgag cctgcgcgat cgcgacctga tctccattca
    10861 agaagcccgc gaactggcgg cacgagctgc cgccgcacaa aagaaattcg ctgaattttc
    10921 ccaggaacaa gtggatgcga ttgtagaggc ctgcgcaaaa gcagcggcag aaaacgtaga
    10981 gcccttagcg cgcgcagcgg tggaagaaac cggatttggc aatgtatccg acaaaattat
    11041 taaaaatacg ctcgctgcgg taaacgtgcc gcgcgcaatt cgcggtatgc gtacggtagg
    11101 aattttgcgc gaggaccgcg aaaagggaat cgttgaagtt gcgtctcccg ttggcgtggt
    11161 tgccgctgta attccgtcga cgaatccaac ttccacggca atttacaaaa cgctgatcgc
    11221 cattaaggcg cgaaacgcga ttgtgttgag cccgcatccc tccgcgatta aatgtatttg
    11281 ccgcgcggcc acggttctcg aacgtgccgc gcttacggcg ggcgcccccg aaggactgat
    11341 tcagtgcatg cagcacacga cgctcgaagg cacgcgcgaa ttgatgcggc ggcctgaggt
    11401 cggcgtgatt ctcgcgacgg gcgggacagg gcttgtccgc gcggcctacg gttcaggcaa
    11461 gccagcctat ggagtagggc cggggaatgt gccggcgttt attgaacgca ccgcggacgt
    11521 gcgtaaggcc gtggcggata tcatcgccgg aaaaactttt gactacggga cgatttgctc
    11581 gtccgagcag gcgattgtcg ccgaagaaac ggtgcgcgaa agcgcgctcg aagagtgccg
    11641 caagcagggc gcttactttt tatcgccgca agaaatcgaa aaaatttcgc gtctggtttt
    11701 tgttcagggc tcgcagacgc cgaacacgga aattgttggc cgcgctgcga cggtgattgc
    11761 ggaaatggcc ggcatccacg cgccacccgc gactcgcgta ctcatcgcgc ggctcgggcc
    11821 tgagcaagtg ggacgcgaat ttccgctctc ggccgaaaaa ctttccccga ttttggcttt
    11881 ttatgcggtg gagaattttg cggccggaat cgcgctgtgc cggcgtctgc tcgaattcgg
    11941 cgggctgggg catacttgct caattcattc gcaaaatcgt gccgcggtac tggaattcgg
    12001 cgcggcgatg cccgcgtttc gcgtcgtggt aaacagcgcc tcggtgcacg gttccatcgg
    12061 atattcgacg aatctttttc cggcgatgac gctcggttgc ggcgcgccgg gcgggaacat
    12121 tacttcggac aacattggtc cgcagcactt gatgaacgtg aaacgcatcg cgtgggaagc
    12181 gcgcgcggtg gaacatcgca cggttcccgc cgatcagcgc atggcgggcg ctgcgaattc
    12241 gcacgaaccc gttgcggtgc aagcggaggt gaacgtgtca actgttcctg aatctataca
    12301 ggcagccgcg cctggcgcgc cagttgtttc gcaggaagcg aaatcagagg agaagaaaat
    12361 tgcagtacct gctgctgcgg catcgactgg tatcgatcgc gttgctatcg cgcgcgtagt
    12421 tgagaatgtg cttgcagcgc agggaattgc tcgcggcagc ggcgtgcctc aatcttcgcc
    12481 tgtgtccgcg gccatgccgg atgcaactgc aagtgaaaca gcgccgaaag aaaaggaatt
    12541 ggatagcccc gcatcgatcg ccgctgaaat tgcgaatcgt ttttttggcg cgacggcaaa
    12601 cgctccgcag aactcgggga agactgcccc ttcgggggag gcccaggctt tgagccagtg
    12661 cccacctgaa ggtgggcgct acatgcttcc ggacgctggg aagaaggttg aacatcccgc
    12721 aaaggcagca atcgaaattc aggtatttgt cagtgaaaac gatgtgcgca gagcgatgac
    12781 gcgcagcgaa aaaatttaca ttgggcgcaa gacgatcctc acaccttccg cgcgggactt
    12841 gggcatggaa catgaagtgt tcgtggaaac cgaaacggga tcgatccgct gatttccatt
    12901 gttgttgtag attttagtag cacaggcttc acaggctgcg gaaaaagtag tttattctgt
    12961 cattccgagc gaagcgagga atctctcttc gatttaagtc taagaaaata aagcaggagg
    13021 gattcctcgc ttcgctcgga atgacaaaat gttgactttt tccgcggcct gttcagcctg
    13081 tggggtttag tccttgcaag ttctaacgcc cacaggctga agagtctgcg tgagaactgc
    13141 aatgttggaa cctcaggggc taaagccgaa cttattttgc agcatttatg tcgtggctaa
    13201 agccacgacc cacaaggatt ttgaagttct cacacagact ctgaagcttg tgctacttta
    13261 cgacctgacg gttggctttc atctgacagc ataaaaggaa aaatagaaat gcgaaaaatt
    13321 tcggttgccg taatttcgct ggcatgcgtt cttctctact cacgcagcgc ggttgcgcaa
    13381 acttccgagg tacacgtgct ggcctcgaac gggatcaaag ctgtgctcga agatctgcgc
    13441 gcgcagggtg agcacgcgat tggccacccg atcacagtcg attacaaacc tactgccaat
    13501 ctgaagcagg atattgatgc gggcgcacag tttgacgtcg tgattgtgac ggtggaaagc
    13561 atcgcccagt taagcaagga agggaagacg ctgggaggag aaggaacgcc tatcagccgc
    13621 gtcggagtgg gcatcggcat tcgcaaggga gcgccacggc ccgatatcag cactccagaa
    13681 gcgctgaagc gggcattgct cggcacaaaa gcgattgctt atggtccagt cggcgccagc
    13741 cttccctata ttacgcacat gctggaatcg ctcggcatcg ccgaggcgat gaaacccaag
    13801 accgtacttt tgaaaacgtc ggacgaaacg aatgaagccg tcgcggatgg gaaagtcgat
    13861 ttcgggatga cgctcgtcag cgaaatcttg ccggcgaaag gcgtcgaact gctcgggccg
    13921 tttcccgaag aatttcaagg ctacgtccgc ttcaatgcag cggtcagcgc caatgcaaaa
    13981 aatcccgaag ctgcgaatgc gctcgttaaa ttcttggcgg gccctgctgc cgcgcctttc
    14041 ataaaagcca ggggcatgca ggcccactga aacgctgaca ttatttgatc gatgggcgct
    14101 gaatgtgcgg ctacattcct gccatttatt ttggatgtaa taaatttgca tctcaacctg
    14161 ccctctcctt tccgaaaggc tcggttcacc gagaacgttc tccgtgaacg ggacggagag
    14221 gcgtgtacga aaccttcgtg ctttgccgtt ggttttgtag cggccgcatt cttttcgggc
    14281 gggcagtatt ttgtcgtgcg tgacgcaaac cgatggacca ttcaaattga tcttgacgcg
    14341 atggaactca cgcagtgtat gattcgtcaa aatttaactt gataagtttt ccggcccggg
    14401 aggaatttct aaatgcgaag tcgtctattg ggtccagctg tggcgctcgc ggttacgctg
    14461 gcctgctctt tttcagcatc cgcgcaaacg gaaaaaccgt ccgacactcc ggccgcctct
    14521 ggtccgtttg atccgcacga tttatccgga gtctggagcc aggagcatcc gcgaaacctg
    14581 cccgtggttg aacgttactg gaattatgaa ttcaacaaag aagagcctcc gatgacggcc
    14641 tggggacaga tgcagttcaa tgccgcgaag tcctcattcg ggcagcacac ctaccctctt
    14701 gcggaaacca atgatccgct ttaccacgac tgcactccgc cggggctgcc gcgcgtttat
    14761 ttgcatcctt tcccaatgga aattgtgcag atgcccggcg aaatcctgat tctttttgaa
    14821 tacgattccc tgcggcatcc aatttatacg gacggccgca agcacgatga agcacttggc
    14881 cccttgtgga tgggtgatgc gattggccac tgggagggcg acacgttggt ggtagatacc
    14941 acgaatttta atgataagac ctggatcgat cgcgtcggac acccacacac tgccgatctg
    15001 caccttgtcg aacacatcaa gcgcacggat cgcactcatt tagcggatga catcaccgtc
    15061 gatgacccaa aggcgtacac taaaccctgg accgcgcact tggattttat tctcaggcct
    15121 acgtggacgc tcggcgagca gttctgcgaa gacgagcata gctttgagaa cttcgataag
    15181 aagggcacga cgagcgcgaa atagtcgacc agagttggca tgaatgtcgc tgaaatcagg
    15241 atgtttatag catttgttcg ttgcggacaa acgtattgac accttttacg ctttagtggg
    15301 atatttcgga tagttggttt tttcttaagc gtagagactc tctttaccac acagtttctt
    15361 tcgggaagac tcagttttta attgatccgc ctttttacgg aggctccgga tatgagaaag
    15421 ctcgcgctct ttgcggtcct ggcatgcgcc gcgctattcg tttcggcgcc actccccgcg
    15481 catcacggcg aagcgaacta tgacaccgat aaaattgtct cggtcaaggg aactgtgacg
    15541 gaattccaat ttatcaatcc gcacacgttg attgcgctgg acgtaaagaa tgacaagggc
    15601 gaaatggaaa agtggaactg cgaagccagg agcccgggaa tgctggtgcg tgccggcggt
    15661 tgggacaagg ctacgctgaa ggtaggcgac gtcatcaccg tatatggtta ccggggcaag
    15721 agtggagcgc tcgtattgcg ccttcaaaag ctaacgctgg cggatggcac ggtgaaggac
    15781 aacctgtagc cgcgggccac tcgctcattt tttggaattc gttttctgct ttacaccccg
    15841 cataccgtaa ttctcttcat tgattttcga caggcgaagc acgcggaggc gttttgaaat
    15901 gcgactggat cgattccgcg ccgcaattgc catccgtttt tttgcaagtt ttcgtgaacg
    15961 ccatgccctg acgggcagac cttccgcgtg aattagtctt gacgcgcttg aacgcgtgca
    16021 gtgtatgatc ctcaaaactg aaccgcaaaa aaagaaactg gaatttgcgt gtgaggagtt
    16081 tcgatatgcg taatcagcca ctgggatttg cggtggcgct cgcaattgca ttgagtattt
    16141 ccccgacgct ttccgctcaa tccaaaaagg cggccgaggc cccggcggcg gataaaggtt
    16201 catttgatcc gcatgatctt tcgggagtgt ggatgcagga ccatccccgg ctgattacgg
    16261 ttgacgcgcg atattgggtg tacaagttca ctctggaaga gccacccatg tccgactggg
    16321 gcgaaacgaa atttaaagca accaagactt ccttcggacc gcacgcattt ccgctagcag
    16381 aaacaaatga tcccgtgtac cacacgtgcg atccgctagg ttttccgcgc atatttcttt
    16441 acccattgcc tttgcaaatc gtgcagacgc cgaccgaagt catcatgctg tttgagtatg
    16501 actggctgcg gcatcaaatt tttaccgacg gacgtaagca tgacgaatcg cttggccccc
    16561 tgtggatggg cgacgcgatt ggacactggg agggagatac gttcgtcgcg gacacgacca
    16621 attttaacga caaaacgtgg ctcgatcgca tgggacatcc gcacagcgat gttttgcatg
    16681 tggtggaacg catccggcgc gttgaccacg atcatctcgt agatgacatg accatcgacg
    16741 atcccaaggc gtacacgaaa ccctggaccg cacatcagat ttttgcgctg caaccgaaat
    16801 ggaccatcgc ggaatctttt tgcgaggatg agcaaagctt cgagtccatc gacgaaagtg
    16861 gagccgcgcc ggtgaagaaa tagaacggtg gctcatcgag atcgaaatgg aatatcaagt
    16921 ttgggaggaa ttttgtaatg cgaaagtgct tgattggctt ggtgatggcg gcgttcgtca
    16981 tttctggttc ttcaagaagt gcatctgccc aaaccaagca gccttcgcag tcgccctcaa
    17041 ccgccggttc atcctcaagt tcattcgacc ctcacgattt gtcaggcgtc tggatgcagg
    17101 atcgtccgcg gatgggcaga gtcatcgagc ggtattggat ttatgaactt acattggaag
    17161 aaccgccgat gacagcctgg ggcctcgcgc aatacaaggc cgcgaaatct tccttcggca
    17221 atcattcgtt tcccatagcg gaaacgaacg atcccatcta tcacagctgc tctcctacgg
    17281 gatttccgcg cgcctttttt catgcattcc ccattcagat tgttcagtcg cccacggaag
    17341 tcgtcgtgtt atttgagtgg gactccctgc ggcatcagat ttttatcgac ggccgcaagc
    17401 acgacgaatc gcttggcccc ttgtggatgg gtgacgccat cggacattgg gagggagaca
    17461 cgctggtggc ggacaccgtg aatttcaacg agaaaacgtg gctcgatcgc gtcggccatc
    17521 cgcacagcga tgcgctgcat gtgatcgagc atattcgccg cgtggaccac gatcatctgg
    17581 aagatgagat tacgattgac gatccaaagg cgtactccaa gccgtggacc gggaaaatca
    17641 tttttgcctt gaagccgaat tggactctgg ccgaggaatt ttgtgaagac atccccagct
    17701 ttgacgcgat tgaaaagcag gagacaactc ccgcaaagta atgactttgg gattgacata
    17761 tctataatcc cagtgggata tttcagtcag ttaagtttcg ggcaacaaaa tgccctgcgt
    17821 tcatccgtct catttgattt ccaacgtgcg ggaggcgtac caagtgaaaa aattggcgct
    17881 cgttctgagc atgtcatttg tttttctctt gatttccgcg ccgatctttg cgcaccacgg
    17941 ggaaacgaat tacgacacgg aaaaacttgt gaccgtaaaa gcgaccgtca ccagctttga
    18001 atttatcaat ccgcacacac agattacgct ggaaacgaaa aacgaaaagg gcgaagtcga
    18061 aaagtggact tgcgaagcaa gaagtcccgc gatgctcgtg cgcaatggtg actggagcaa
    18121 gaatacgcta aagcccggcg acatgatcac agcaatcggt tttcgagcca agaatggggc
    18181 aaacattctg cgccttaaaa agattgtgct gtcggatggc aaggaactac ccgatctata
    18241 gaaatgtgct tttgtagcac gcctcttcac aggcgggggt tttggtgacg catcatctaa
    18301 acccccgcct ctgaagaggc gggctacagc aaatttaaat ggtgcaaaga tccacgaaga
    18361 ttcaggttgc gaggtttttt caatgaacct taggccggcg gctctctttt tcttgcttgc
    18421 gggactttta ttggttagcg ctcccgtgtt cgcgcatcat ggcaactccg cttatgacga
    18481 ggctcactgg attactattt ccggcaccgt gactgaattc gtttggtcca acccgcattg
    18541 ccagattttt gtggatgtga agggcgacaa aggaaaagtt gtgaactggg cgattgaaag
    18601 ccagagccct ggaattatgc gcagaaataa ttggacgagg aattccctcc agcccggaga
    18661 ccagattacc gttacgctcg cgcccgctaa aaatggagct gctgtaggat tttccgggaa
    18721 tgccgacggg aaggttgtat tcgccgatgg acgcgtcttg aagatgaaca atcgataaaa
    18781 atagcggcgc gggcctcaat gcgtttgcct ccgtcaccgc agccgcaata aattcggcgt
    18841 tagatttttt gccaacgata ggagcaccaa agtgcgaagt cggccaccag tgtggatcgc
    18901 gtgttcgatg gcggcactga cggcagcgtt tgcaatctcg accattgttc atgcccagtc
    18961 gaatcttccg caaggtacgc cgaaatccgc cgctgcgcct gctcccgctc cgaaacgtga
    19021 tttaacgggc gtctggcaac ttcagggagg caatggcggc gcgcagccca ttgcggagga
    19081 caaggacatg cctccgatga cgccgtgggc aaaagtaaaa tttgacgcgc agaaaccggg
    19141 ctatggttca aggggtgccg cgggtggcaa cgatcccatc ttgcaatgcg atcccatcgg
    19201 ttttccgcgc atcatgttga tgcctactcc gaatgaactt ctgcaggcac ccgggcgcac
    19261 aattcagttt tgggagcgcg aacacgaatg gcgcaccatc tggaccgatg gacggccgct
    19321 tcccaccgac cctgatcccg cctggtatgg atacgcgatc gggcactggg aaagcgatga
    19381 tactttcgtc gtggaatcga tgggcttcaa tgacaagacg tggctcggcc ctacgggata
    19441 tccgcacagt gaagagatgc gcgtggtgga aaggtatcgc cgcgtggacc acgacacaat
    19501 tgcttatgac atgaccatca ccgatccgaa ggcctacgcc aagccggttg tcacgccgcg
    19561 ccgagtgatg aagctgaagc ccaacgatga gatcagcgag cagccctgcg tatggtcgca
    19621 ggaaaacgaa tttgcgcaga gaatcagaaa tcccgcgacg cccaagtccg caaaataaaa
    19681 gcaggacagg catcatggcc cgtgtgagaa ctgcaaaatt ggaacttcag cggctaaaca
    19741 gtttgcggaa aaagtagttt attctgtcat tccgagcgaa gcgaggaatc tctcttcgat
    19801 ttaagtataa gaaaataaag aaggagggat tcctcgcttc gctcggaatg acaaaatgtt
    19861 gagtt
//
LOCUS       DASZMB010000251         3703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3548603, whole genome shotgun sequence.
ACCESSION   DASZMB010000251 DASZMB010000000
VERSION     DASZMB010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3548603"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..1603)
                     /locus_tag="VG272_11770"
     CDS             complement(182..1603)
                     /locus_tag="VG272_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWF14408.1"
                     /translation="MASPEASDAKQQLRRVMGFWDVLLFCIATVLGPRWIAAAARNGQ
                     SSITLWILAALLFFVPTAYIVVELSTRFPEEGGLYVWTKMAFGDFHGFVAGWTYWIYT
                     VFYFPGLLLASAAMSAYVGGAKTAWMAQNRTFLLGGSLVLLVVAVWLNIIGLHIGKWL
                     QNAGGIGTYLPLMMIVGVALLLWSRHGSVTHFTLRGMLPHWDWDTVNFWPQLAFAFAG
                     LELVSAMSEEVKNPQRTFPRAIFASGALIALMYIAGTVAVLMMLPSETVDPKSGVFQA
                     ITEGSTVLRMMAVGVIAALLVTVGNAGGVGSTVAGVARIPFIVGIDRYLPAAFGKIHP
                     RWKTPYISILVQACISGVILLVAQINENTRGAYQILVDAATILYFIPFIYMYAAALKL
                     YAKPDRRENRSAVVIPGGRLGVCVAGGLGLMVVCVGIVLSFIPPGDTSNKVLFETKLV
                     AGTVLSILLGLVLYYRGAANREK"
     gene            complement(1606..2406)
                     /locus_tag="VG272_11775"
     CDS             complement(1606..2406)
                     /locus_tag="VG272_11775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWF14409.1"
                     /translation="MGQPTQPLSPQAATGHFIEFRNVSKSFNDHNVLDDVSFFVDHGE
                     TSVIMGRSGVGKSVTLKLILGFLHADSGRIFVGGKDITDWNEDELTEIRRRVTMVFQS
                     GALFDSLTVSENIAFPLETRTDLTEEEKETRVKEFSEMLEVTNIADKLPSELSTGMKR
                     SVAIARALAQNPEAILYDEPTTMVDPLMAVHTANLILRLKEKFHKTSVVVTHDTHLAK
                     KLADRIVFLHEGRVAYFGPWAGFVAAPEPFLQNFLRQDQLTPALDVTS"
     gene            2968..3288
                     /locus_tag="VG272_11780"
     CDS             2968..3288
                     /locus_tag="VG272_11780"
                     /inference="COORDINATES: protein motif:HMM:NF001533.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HWF14410.1"
                     /translation="MSVNVTPLHDRVLIKRIEEKESIKGGIIIPDSAKEKPQEGEVVA
                     AGAGKIEKGVRVPLDVKVGDRVLFGKYSGSEIKVDDEDYLILREDEILAKLSGAAKAA
                     GGKK"
     gene            3438..>3703
                     /locus_tag="VG272_11785"
     CDS             3438..>3703
                     /locus_tag="VG272_11785"
                     /inference="COORDINATES: protein motif:HMM:NF012346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HWF14411.1"
                     /translation="MAKQIVTGEASRQSILRGVNALADAVKITLGPKGRNAVIEKKFG
                     APIITKDGVTVAKEIELQDPLENMGAQMVREVASKTSDVAGDGTT"
BASE COUNT          934 a         1075 c          929 g          765 t
ORIGIN      
        1 aactcaacat tttgtcattc cgagcgaagc gaggaatccc tccttcttta ttttcttata
       61 cttaaatcga agagagattc ctcgcttcgc tcggaatgac agaataaact actttttccg
      121 caaactgttt agccgctgaa gcttcaattt tgcagttctc acacagattc tgaagcctgt
      181 cctacttttc tcgattcgct gccccgcggt agtacaaaac caatcccagc aagatcgaca
      241 gcaccgttcc cgccaccagc tttgtctcaa acaaaacttt gttagatgta tcgcccggcg
      301 ggataaatga cagcacaatt cccacgcaca cgaccatcag tccaagcccg cccgcaacac
      361 acacgcccag ccttccgccc ggaatgacga ccgccgagcg attctcgcgc cggtccggct
      421 tggcataaag cttgagcgct gccgcgtaca tataaataaa tggaatgaaa taaaggattg
      481 tcgcggcgtc caccaaaatc tgatatgccc cgcgcgtatt ttcattgatc tgcgcgacca
      541 ggagaatcac gcccgatatg catgcctgca ccagaatcga aatgtacgga gttttccagc
      601 gcggatgaat ttttccaaac gccgcgggca gatagcgatc gatgcccaca ataaacggaa
      661 tgcgcgccac gccggccacg gtcgagccca cgccgcccgc gtttcccacg gttacaagca
      721 gcgcagcaat cacgccaacc gccatcatgc gcagtacggt cgagccttcc gtgatggcct
      781 gaaatacgcc acttttcggg tcaactgttt cgctcggcaa catcatcagc acggcaacgg
      841 tcccggcgat gtacatcagc gcgatcagcg cccccgaagc aaaaatcgcg cgcggaaatg
      901 tacgctgcgg atttttcact tcctcgctca tcgcggaaac gagttcgagc cccgcgaacg
      961 caaatgccag ctgcggccag aaatttaccg tgtcccaatc ccagtgcggc agcattccgc
     1021 gcagcgtaaa gtgagtcacc gatccgtgcc gcgaccataa gagcagcgcc acacctacga
     1081 tcatcatcag cggcaggtag gtgccaatgc cgcccgcgtt ctgcagccat ttcccaatgt
     1141 gcaggccaat aatattcagc cacaccgcaa ccacgagcag caccaacgat ccgcccagca
     1201 aaaatgtacg gttttgcgcc atccatgcgg tcttcgctcc gccaacataa gcggacatag
     1261 ccgcgctcgc gagcagcaat cccggaaaat aaaaaaccgt gtaaatccaa tacgtccatc
     1321 ccgcaacgaa tccgtgaaaa tcgccgaagg ccattttcgt ccaaacatac agcccgcctt
     1381 cttccggaaa tcgcgtggaa agttctacaa caatgtaagc agtgggaaca aaaaacagca
     1441 gtgccgccaa aatccataac gtgatggaag attgtccatt gcgcgccgcc gccgcgatcc
     1501 accgcggccc cagcaccgtg gcaatgcaaa acagcagcac atcccagaaa cccatgacgc
     1561 gccgcagttg ctgcttggcg tcagaagcct ctggcgaagc catgctcacg aggttacgtc
     1621 cagcgcggga gtgagttgat cttgacggag aaaattttgg agaaatggtt cgggcgcggc
     1681 tacgaaacct gcccatgggc caaaataagc gacgcggcct tcgtgcagaa aaacaattcg
     1741 atctgccagt tttttcgcca gatgagtgtc gtgcgtcacg acgaccgatg tcttgtgaaa
     1801 cttttctttc agccgcagga tcaagttggc agtgtgcacc gccatcagcg gatcgaccat
     1861 cgtcgtcggc tcatcgtaga gaatcgcttc cggattctgc gccagcgccc gcgcgatagc
     1921 caccgagcgc ttcattcccg tgctgagttc ggacggcaat ttgtctgcga tgtttgtaac
     1981 ttccagcatt tctgaaaatt cttttacgcg cgtttccttt tcttcttcgg ttaaatctgt
     2041 tcgcgtttcc agcggaaagg caatattttc cgacaccgtc agcgaatcga acagcgctcc
     2101 cgattgaaaa accatggtga cacgcctgcg aatttccgtc agctcgtctt cgttccaatc
     2161 cgtgatgtcc tttccgccca caaagatgcg cccggaatcc gcgtgcaaaa atccgaggat
     2221 gagcttcagc gtcaccgact tccccactcc gctgcgcccc ataatcacgc tggtttcgcc
     2281 atggtcgaca aaaaaactta catcgtcgag cacgttgtgg tcgttgaacg atttgctcac
     2341 gtttcggaat tcaatgaagt ggccggtggc ggcctgcggt gaaagcggct gggttggctg
     2401 gcccatgtca ggtcactatt gttgatgagc cgaagcgggg aagcaaacca aattcgctac
     2461 gttgtaactc aagattcagc gctcgccttt tgcgattcca tactcagtcc cgcagggacg
     2521 aaacatcgta gcccaccgtg aaacggtggg taggttcgaa ggtgaaagca gagccccgtg
     2581 gggcggcaca aaattcccat acgtgtgccg tccctgcggg actgatttaa acgcggtatg
     2641 tcgaaccctc gtgtcagcgg agaccgataa gagcgaatcg ctgcaaggac gcggaaaaaa
     2701 atccacaggc cgaaggcagt cccattgaac ccggagcact tgcgcctcta tcgaattgaa
     2761 aacaatagac atgcaggaac aaatgttcct tccagctctc gcgttattac aggaacttaa
     2821 cttgacttcc caccctcttt tgctatatta gcactcgtct tcgaggagtg ctaaccattc
     2881 accgagaacg tttttctgtg aacgatctgc tcagcactct ctcctgacca taccgactta
     2941 ccctaatttg aacggaggca cgaaacgatg tcagtaaacg ttactccgct gcatgatcgc
     3001 gttttgatca agcggattga agagaaagaa tccatcaagg gcggcatcat catcccggac
     3061 tcggcgaaag agaagccgca agagggcgaa gtggtcgctg ccggcgcagg caaaatcgaa
     3121 aagggcgtgc gcgtccctct cgacgtaaaa gttggcgacc gcgtgctgtt cggcaagtac
     3181 agcggcagcg aaattaaagt tgacgacgag gattatctga tccttcggga agatgaaatc
     3241 ctggcgaaat tgtccggcgc agccaaagct gccggcggca agaagtaact ccctgccctg
     3301 agcgcaaccg cgcgaagggt ttacccctgc ctctcttcac agagggcgca aggccgcaag
     3361 atctggaaat tctcaatttt gaaatttcaa atttgagatt tgaaattgaa atcatggaaa
     3421 gaagaggaga ttgactcatg gctaagcaaa tcgtgactgg agaagcatcg cgccagtcta
     3481 ttctgcgtgg cgttaacgcc ctcgcggacg cggtaaagat caccctcggc cccaagggcc
     3541 gcaatgcggt gattgaaaag aaattcggcg cgccgatcat caccaaggac ggcgtgacgg
     3601 tagcgaagga aatcgagctg caggatccgc tcgagaacat gggcgcgcag atggtacgcg
     3661 aagtcgcttc caagacttcc gacgtggccg gcgacggcac cac
//
LOCUS       DASZMB010000252         6396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1889116, whole genome shotgun sequence.
ACCESSION   DASZMB010000252 DASZMB010000000
VERSION     DASZMB010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6396
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1889116"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            410..2566
                     /locus_tag="VG272_11790"
     CDS             410..2566
                     /locus_tag="VG272_11790"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HWF14412.1"
                     /translation="MPVQARPKTAKHDSSRYHGLSRGDLLRIYRTMFLSRRIDDREIQ
                     LKRQHKIFFQISGAGHEGVLAAAGLVLRPGHDWVYPYYRDRALCLALGVTSQEMFLQA
                     VGAKDDPHSGGRQMPSHWGIGRLNILPRSSSTGMQYTHAVGMAEASLYFEKFPRALEQ
                     AQKAPLGEHVRHQPDEIVYVSGGDGSTSEGEFFEALNMACLRQLPILFLIEDNGWAIS
                     VPVKYQTPSGNIAKLVASYPGLFVEECDGSDPLASYVVLQRAAEYCRERKGPALVHAQ
                     VTRPYSHSLSDDEKLYKSDSVREEEAHRDPIPKFGLYLVREGIVDEKELEAIEAEVDR
                     EVREASDQALAAELPSQECILVNQYSHDIDPTSSRFESAPTFSGEPRTMVEMVSHTLA
                     DEMSRDERIVVLGEDVADCSNEEDLKHLKGKGGVFKATVGLQRKYGYARVFNTPIAEA
                     CIVGSAIGMAARGLKPVAEIQFFDYIWPAMMQLRNEMSLFRWRSNGAFKAPAVIRVAT
                     GGYLTGGAIYHSQSGESLFTHTPGIRVVMPSTALDLCGLLRTAIRCDDPVLFLEHKHL
                     YRQPYNRSAYPGPDYTIPFGRARVAREGTDVSIVTYGAVVHRAEVAAAQLERDGISVE
                     IIDLRSLSPFDWDAIATTVCKTNRVIVAHEDMISWGYGAEIAARIADQLFDELDAPVR
                     RVAAMDTFCAYQPHLEDVILPQTGDLTAAVRELVAY"
     gene            2771..3280
                     /locus_tag="VG272_11795"
     CDS             2771..3280
                     /locus_tag="VG272_11795"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HWF14413.1"
                     /translation="MYVPQSAHPPEVISAIPEIPVLPPRPGFAGFWLRAIACFIDTIF
                     IVAIFLTGASFFPATFEKLLPPASASFTDFPQPAPIVIVILIALGCVYYTVFESSVWQ
                     ATPGKRILRLYVTDLNGQRITVGRALLRNIARQISGFLFIGYVMAGLTEKKQALHDLI
                     AGCLVVRKR"
     gene            complement(3509..3943)
                     /locus_tag="VG272_11800"
     CDS             complement(3509..3943)
                     /locus_tag="VG272_11800"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWF14414.1"
                     /translation="MQFLDNLKPLGLLVLRVALGATFIFHGYPKLTDPEHALKAFAGY
                     GFPGYFAYIFGIVEVFGGGLLIAGLFTRGVGLLLTIETGLILYKSIIPSEGMRGFGRF
                     EMPLLLGAMAFALVTTGAGVISIDVFTFESGRKSAKKVKVKN"
     gene            complement(4041..5015)
                     /locus_tag="VG272_11805"
     CDS             complement(4041..5015)
                     /locus_tag="VG272_11805"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HWF14415.1"
                     /translation="MTLLLTETDVRSLLTMPIALEIVEESLRQQGKGELILNPRRRIK
                     TPDNALLHYMAAADPVRGYFGMKIYTVARGTARFVVPLFRSTTGEMVALVEADALGQL
                     RTGAASGVATKYLANPNASTACIIGTGYQARTQLEAVADVRPLKRVRAFGRDPERRAK
                     FCSEMSETIGVTVEPANSGEDAVKGAEIIITATSATKVVLEGAWLTPGMHINAMGANW
                     PQKRELDAAAVGRAGKIVVDSIEQSKMEAGDLVQAFGEDISRWNAVQELSEIVVGKES
                     GRTSTGQITLFKSNGIATWDLAAAVRVYEMAVARGIGQLIPLWEAREG"
     gene            complement(5012..5983)
                     /locus_tag="VG272_11810"
     CDS             complement(5012..5983)
                     /locus_tag="VG272_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HWF14416.1"
                     /translation="MAQMNSDSIRSRFPYAALFAIFFLVSCGNRANPAVKPQAPPPPP
                     FEFLGSWGDKGEGPGKLDAPVAFASDALGNIFFADPAIDYVHKFESKGTPLLSFEDPR
                     LRHAAGISIDAGGAIYVVDPQLGNIHVFFPDGTFFQSWHVAPQRHFSGPVDVGIDEVG
                     TIYSPDFANSRIQKFNNHGRLVTSWPAPQKPVSPEERPSWVAAEPDNAIFVAYFNTGR
                     VEKFSPEGTSIVSWPAGDMTAENSGVLSALAVNTDFVFTMGASSSEIRVWDTDGRHKL
                     DADLTANLGKIAAPQLAITPHGELLVFDPSAQKVFRFRIHLPTKEPL"
     gene            complement(6194..>6396)
                     /locus_tag="VG272_11815"
     CDS             complement(6194..>6396)
                     /locus_tag="VG272_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14417.1"
                     /translation="RKRYETAYVESDLCGRLPIQITAGAKGPEFQFPDVDQGGAKRMY
                     GMYQTSIRLIKDRIAQAAQSTH"
BASE COUNT         1417 a         1677 c         1762 g         1540 t
ORIGIN      
        1 agcgcggcga gcgtctccat cccggcacca caccccggga cggagcgctc gccgcgattt
       61 tttttatcca gaaggcttga aaccaccgat gcacacggat aaacacggat ggatgaaaga
      121 taaattcaaa ttcgattcac ccacattctg caattttcga tctcttatca aatccctcta
      181 aatattgagt tttgattttg cgtatcaatt gttttatctg tttttaatcc gtgttcatcc
      241 gtgtgcatct gtggttccaa cctttggttt ttgcgccgag gtgtgcgcta tccggttggc
      301 cactcatttg agaataattc cttccctgta ttcgcgcact gcccaggttt ggatataatg
      361 agatgtactt ccgaaactag ctgtcttgag acagctcaag gtgattcgga tgccggttca
      421 agcgcgccct aaaactgcga aacatgactc gagccgctac cacggattgt cccgggggga
      481 tcttcttcga atttaccgca cgatgttcct ttcccgccgg atcgatgacc gcgaaatcca
      541 gctcaaacgt cagcacaaaa ttttttttca gatcagcggc gcggggcacg aaggtgttct
      601 ggccgctgcg gggctggttc ttcgtccggg ccatgattgg gtgtacccat attatcgtga
      661 ccgcgctctc tgcctggcgt tgggcgtcac atctcaggaa atgtttcttc aggccgtggg
      721 cgcgaaggac gatccgcatt ccggcgggcg ccagatgcct tctcactggg gcattggccg
      781 gctgaatatt ctgccgagat cttcttcgac gggaatgcag tacacgcatg ctgttggcat
      841 ggccgaagcg tccctttatt ttgaaaaatt cccgcgcgct ttggagcagg cgcagaaagc
      901 gccgctcggc gagcatgtcc gccaccagcc tgacgaaatt gtgtacgttt ccggcggcga
      961 tggatccaca agcgaaggcg aattttttga agccctgaat atggcctgcc tgcgccagct
     1021 tccgatttta tttttgattg aggataatgg ctgggcgatt tcggtcccgg tgaaatatca
     1081 gacgccgagc ggcaatattg cgaagctggt ggcgagttat ccaggccttt tcgtggaaga
     1141 gtgcgacggc agcgatccgc tggcgagcta tgtagttctg caacgcgccg cggaatattg
     1201 ccgggagcga aaggggcctg cgcttgtgca cgcgcaagtg acgcggccct actcgcactc
     1261 gctttccgat gacgaaaaac tttataaatc tgatagcgtg cgagaggaag aagcgcatcg
     1321 cgatccgatt ccgaagttcg gtttgtatct ggtgcgcgag ggaatcgttg acgaaaaaga
     1381 attggaagct atcgaagccg aagtcgatag ggaagtacga gaagcatccg accaggcact
     1441 cgcggcggaa ttgccttccc aagaatgcat tcttgtaaat caatattcgc acgacattga
     1501 tccgacatca tcgcgatttg aatccgcgcc gactttttcg ggcgagccgc gcaccatggt
     1561 ggaaatggtt tcgcacacgc tagccgatga aatgtcgcgt gacgaacgta ttgtcgtgtt
     1621 gggcgaagac gtggccgatt gcagcaatga agaagacctg aagcatttga aggggaaggg
     1681 cggcgtcttc aaggcaactg tcggtttgca gcgcaaatac ggatacgcgc gcgtgtttaa
     1741 tacgcccatt gcggaggcat gcatcgttgg cagcgcgatt ggcatggcag cgcgcggatt
     1801 aaagccggtc gcggaaatac aatttttcga ttacatctgg ccggccatga tgcaattgcg
     1861 caacgaaatg tcattattcc gttggcgctc gaacggcgca ttcaaggcgc cggccgtgat
     1921 tcgtgtggct accggcggtt atctgacggg tggcgcgatt tatcacagcc agagcgggga
     1981 atcgctgttt acgcacacgc cgggaattcg cgtggtgatg ccttcgacgg cgctggacct
     2041 gtgcggcttg ctgcgtacgg cgattcgctg cgacgatcct gtgttgttcc ttgaacacaa
     2101 gcatctttac cggcagcctt acaatcgctc cgcgtatccc ggaccggatt acacgattcc
     2161 gtttgggcgc gcgcgcgttg caagagaagg caccgatgta agcatcgtca cgtacggagc
     2221 cgtcgtgcat cgcgccgaag tggctgcggc gcaactggaa cgagatggca tctccgtgga
     2281 aattatcgat ctccgatcgc tctctccatt tgattgggac gcaattgcga cgactgtttg
     2341 caaaaccaat cgcgtgatcg tcgcgcatga agacatgatt tcgtggggat acggcgcgga
     2401 aattgccgcg cgcatcgccg atcagctttt cgatgaactg gacgcccccg ttcggcgcgt
     2461 cgctgcgatg gatacattct gcgcgtacca accacatctg gaggatgtaa ttcttccgca
     2521 aactggcgac ctgaccgcgg cagtccgcga actggtcgca tattaaggcg agcagcactc
     2581 ttcccttgaa tcgcgctaag cccattggcg ttctctcatt gcaaagcgca tcttccactg
     2641 ttaggttcct taaggcgcgt tctggaggct cgcaattctg acttgtgaaa aatgtggtgg
     2701 gggcatcccg gatggagcga gtttctgcgg tcaatgtcaa actccggtaa gtgtcgcgca
     2761 gattgggcct gtgtatgttc cgcaaagtgc gcatcctcct gaagttattt ctgcgatacc
     2821 ggaaattccg gttctgccgc cgagacctgg gttcgccgga ttctggctgc gcgcgattgc
     2881 ctgcttcatc gacaccattt ttatcgttgc catatttctc accggcgctt ctttttttcc
     2941 ggccactttt gagaagctac ttcctcccgc ttccgcatcc tttacggatt ttccgcagcc
     3001 tgcgcctatc gtgatcgtta ttttgattgc tctggggtgc gtgtattaca ccgtgtttga
     3061 atcatcggtt tggcaagcaa cgcccggcaa acgaattttg cggctgtacg tcacggacct
     3121 gaatgggcag cgcataactg tagggcgagc cctgcttcgc aatattgcca gacaaatttc
     3181 aggtttttta tttattggtt acgtgatggc gggtttgacg gaaaagaagc aggctctgca
     3241 cgacctcatc gctggttgcc tagtggttcg taaacggtag agagagtcag tagtgaatcc
     3301 cgtagaattg taaatgcgga acagaagtcg ccctcccaaa aaacggccag ccgctacaaa
     3361 accaagcacc gcgatttgcg atttcgtaga gccttctccg tttcgttcac ggtgaacgtt
     3421 ctgggtgaac cgcgcatttc ggaacggaga gggcatgttg cgctaacaaa tgcgcgctga
     3481 gaaaaaaatg gaatggcgat cggtccgctc aattcttaac tttgactttc ttggcggatt
     3541 tgcggcctga ctcaaacgtg aacacgtcga tggaaattac tccggcgccg gttgtgacga
     3601 gcgcaaaagc cattgctccc agaagcagtg gcatttcaaa gcgtccaaat cctctcattc
     3661 cttctgatgg aataattgat ttgtagagga tcaatcctgt ttcaatcgtt agcagcaagc
     3721 ccacgccgcg cgtgaacaac ccggcgatta aaagtccgcc gccaaaaact tccacaattc
     3781 caaaaatgta ggcgaaatat cccgggaaac cgtatcccgc aaaggccttt agcgcatgct
     3841 ccgggtccgt cagtttcgga tacccgtgga aaatgaaagt cgcgccgagt gccacgcgca
     3901 ggacgagcag tcccaacggt tttaaattgt ctaaaaattg cattcttctt ttctccccaa
     3961 gcgagaacta aagttaaaaa tctcagtctt tgtagtaaat gatccgtttt tagtgcgcgg
     4021 gtttttagtg cttacaagga ctaaccctcg cgggcttccc agagcggaat aagttggcct
     4081 atgcctcggg cgacggccat ttcatatacg cggacagctg ctgccagatc ccatgtagcg
     4141 atgccgttgg atttgaataa agtgatttgg ccggtactcg ttctgccaga ttcttttcct
     4201 acgacaattt ctgaaagttc ctgcactgca ttccatcttg aaatatcttc gccaaatgct
     4261 tgcaccaggt cgccggcttc catctttgat tgctcgatgg aatcgacgac aattttgcca
     4321 gcgcggccaa ccgccgcagc atcgagctcg cgcttttgcg gccaattcgc gcccatagcg
     4381 ttgatatgca tgccgggagt gagccacgct ccctccaaga caactttggt tgccgaagtt
     4441 gctgtgataa taatttccgc gcccttcacg gcgtcttcgc cggaatttgc gggttccact
     4501 gtgacgccaa ttgtttcgct catttcgctg caaaatttcg ctcgacgttc gggatcgcgt
     4561 ccgaaagcgc gaacgcgctt aagcggacgc acatctgcga cggcttcaag ttgtgtccgc
     4621 gcctgatatc ccgtgccaat gatgcacgcg gtgctcgcat tcggattggc gaggtatttt
     4681 gtagcgacgc cgctagccgc gccggttctg agctggccga gagcgtcagc ttcgacgagc
     4741 gccaccattt cgccggtcgt cgagcggaaa agaggcacca cgaaacgcgc tgtgccgcgc
     4801 gctacggtat aaattttcat gccgaaatat ccgcggaccg ggtcggccgc agccatgtaa
     4861 tgcaacaggg cattgtcagg cgttttgatt cgacggcgcg ggttgagaat taattcgcct
     4921 tttccttgtt ggcgcaggga ctcttccaca atttccagcg caatcggcat ggtcaaaagg
     4981 gagcggacgt cagtttcggt cagcagcaag gtcatagcgg ttccttagtt ggcaggtgta
     5041 tccgaaagcg aaatactttt tgtgcggaag gatcgaacac gagcagttcg ccgtgaggcg
     5101 ttatggccaa ttgtggcgcg gctatttttc caagattcgc agtcaaatcc gcgtccagtt
     5161 tatgccgccc atccgtatcc caaacgcgaa tctccgacga cgaagcgccc atcgtaaata
     5221 cgaaatccgt gttcacagcg agcgcggaaa gcacacccga attttctgca gtcatatccc
     5281 ccgcgggcca ggagacgatc gaagtccctt ccggagagaa tttctcgacg cgcccagtat
     5341 tgaaataggc gacgaatata gcgttgtccg gttccgcggc aacccaggaa ggccgctctt
     5401 ccggcgaaac tggcttttgc ggcgcaggcc acgaagtgac aaggcggcca tgattgttaa
     5461 atttctggat gcgcgaattg gcaaagtccg gcgagtagat tgtgccgact tcatcgatac
     5521 caacatccac aggcccggag aaatggcgct gcggcgcgac atgccaggat tgaaaaaagg
     5581 ttccatccgg gaagaaaacg tgaatgtttc caagctgagg atcaactacg taaatggcac
     5641 cgcccgcgtc gatggaaatg ccggcagcat ggcgcaggcg cggatcttca aacgagagca
     5701 acggagtgcc tttggattca aacttgtgga cgtaatcgat ggcaggatcg gcaaaaaaga
     5761 tattgcccag cgcgtcggat gcaaaggcta cgggagcatc cagtttgcca gggccctcgc
     5821 ctttatcacc ccatgatcct aggaactcaa aaggtggagg cggaggtgcc tgcggtttta
     5881 ccgcgggatt cgcgcggttt ccgcaggaca ccaggaaaaa tatggcgaag agggcagcgt
     5941 acggaaatct cgaacggatc gaatcggaat tcatttgtgc catttgaaaa atattaatcc
     6001 attgatcgta gaaacttttc gacaaggata ttaccacgcg ctttgcgaag ggaggtgcgg
     6061 cacgcgcggg gagtgttcac aagaattatg tggcgtaggc ttcagcctgc gggggttagt
     6121 gcatgcaagg cccaacccct gtaggctgaa gcctacgcca caaaatgcca gataattccg
     6181 cggctagctg cgattagtgg gtgctctgag ctgcttgtgc gatgcgatct tttatgaggc
     6241 ggatactcgt ctgatacatc ccgtacattc ttttcgcgcc gccctgatct acgtccggaa
     6301 actgaaattc cggccctttt gcacccgctg ttatttgaat gggcaggcgt ccgcagagat
     6361 cgctttccac gtacgcggtt tcatatcgct tgcgtg
//
LOCUS       DASZMB010000253        15917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3805719, whole genome shotgun sequence.
ACCESSION   DASZMB010000253 DASZMB010000000
VERSION     DASZMB010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 15917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15917
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3805719"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1650
                     /locus_tag="VG272_11820"
     CDS             <1..1650
                     /locus_tag="VG272_11820"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HWF14418.1"
                     /translation="DVNFQISEGERYTVADLKVDDHDHNTTGTIGDRHFLNLFPGKPF
                     SPALLQQDRNTILAGYLNRGYENAQFHSTFTAEPTNAHLIDVVYTIDPGPQARISDTV
                     ALGTEVTSQSFLHDITGPEIRRGKPISLGNFLTSESDLYNLSIFDWASIKPLAPVSDQ
                     EQEEVLVKVHEAPRNSVDFGGGFEVLPRSGNIPVGTVALPGIPPIGVGSRFTASQQSF
                     WGPRVSIQFARHNLRGRAETATLGLVYSRLDQRGTFTYTDPRVRGSSWNSLFSLSAER
                     TTQSSIYTGELAQASLQVERFFDKKKTKNLILRYTYQRTLLTNITIPDLVLPEDQHVR
                     LSTVAAQYVHDTRDKPLDAHHGIYQTATLEISPTKLGSSVNFLRFFGQTSFYKPLTPW
                     LTWANNFRLGLAKPLAGGTIPLSERFFSGGADSIRGFPINGAGPQRPVSVCSNPSDAS
                     TCTLISVPVGGNMLAVVNSEARFPIPLKSGLGGVVFYDGGNVYNRINLREFADNFTHT
                     VGFGFRYETPVGPVRFDIGRRLTSVPGVTATEYFVTLGQSF"
     gene            1691..5647
                     /locus_tag="VG272_11825"
     CDS             1691..5647
                     /locus_tag="VG272_11825"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HWF14419.1"
                     /translation="MSGKRVVRWTAIVLGGLLAALVIAAIVVVHTTAFQRFVVSQLEQ
                     KVQAGTGARLDIKGLSINWRLLTLDLYGITLHGRETDPQPPLLAADHLRVGLKIISLL
                     RQKVDLREITLDHPAAHFTVDRSGVSNLPQTNSSGTTSSAVDSILQLTIGHLQLNSGV
                     LYYNNAQIPLNADVRDLRADVHYNPISSEYAGTVGYNDGRIATGGLKQVPHRLQLNFS
                     LDRSGIVASPFTIVTAQSTLNVRAKLTNYDHPQLDGTYDAALSTQELARYLNNASLPA
                     GDVALSGRLHFENVANKSALESAYVDGQLKSQQLLVDIGQMRAIPKSIHAEFKLQDGN
                     LSVNKVEADLLGGHVSGDYQLSHLSGNSSSHARALLRDVSLQQLDRTAKIQKGKPLKI
                     GGRVNGTAEAMWSSSVQNAVVKANFDIRSPNSSSALEGVIPLSGLIHVAYDGAQNSAS
                     FGQSYLQTGNTKITVSGVLSRHSRLSVQANTTDVHEVAALVSFVQAGNSANATGAGSS
                     LPDVGGAARFMGEVSGAIQQPRIRGDISASGLQVKNAKWRTFHATLDASASGVTFQNA
                     SLVDLQNGQINFSGHADLSAWSFTPASPLTLQLSARQISISELQEFFQSQYPVSGVVA
                     ANLSIHGSEQNPEGQGSLQITKATAWNEPIKDLSVNFRGDGNTVHTDAQLAIAAGNLA
                     ATLTYQPKTQTYDTKLTTAGLKLDQLESVQARNLGVAGDLALSAQGSGTFQSPQFTAD
                     LKIPKLAIRNQTVSDVQAQLSLANQRATFTVNSRADQGTIQAKGNVGLSGDYPATASI
                     DIRAIPVALVLARYLTKTQDIQGQTEIHLNLDGPLKNPYAIIAQLEIPTLNVNYETAK
                     IGLVRPLKASYRNGVVTVDQAELKGTGTDLNLKGVIPIKSSAPLNVSAAGSLDLSVLQ
                     AFTTGTKSSGNINLDISASGDFLHPAMRGNIKIVNAAIATETLPVSIERLNGEIQVSG
                     NRLEVSQLSGAAGGGTVAVRGFMTYGSQSNFNVGLDANNVRIRYPEGIRSVLSGNLSV
                     AGSPASSQLSGRVLIDRLSFTQQFDLATLLGGFSSDSPSTTSSPLESNMKLNVAVATA
                     QDINLTSSKVSMGGAANLTLSGTVANPVVLGRATLTSGEMFFMGKRYEIQNGTIEFAN
                     PVRTMPVVNLYVKTTVQQYNITLNFVGPVDKLRTNYTSDPPLPPADIINLVAFGKTAE
                     EAASSSSTPASLGAESVLAQGAASQLSGKLEKLAGISQLTIDPLAGNSQSNPGAQVAI
                     QQRVTGKILLTFSTDVTSTQNQAIQVQYQIKKGLSVSVLRDQYGGYAADVRIHKTF"
     gene            6118..7209
                     /locus_tag="VG272_11830"
     CDS             6118..7209
                     /locus_tag="VG272_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14420.1"
                     /translation="MKLSTIGRLAAGLSLTVAGACLLSGTHAQPKPADSGYHLLKTIS
                     LPAAPGGAEYYDYITIDAESRRVYISHGTEVVVLNADDYSVAGKIEGLSRSHGIAIAK
                     DLGKGFITDGDSRDGATVQEAVVFDLKTLKVTNHVKTGQLDTDAIIYEPVNKKIFTFN
                     GDSHNTVVIDAVKESVITTIDLIGKVEFPAVDGKGLVFDNNPEKNDIVVIDAKTNTIK
                     TRYPTAPEGNPTSMAIDQKTRRLFSGGRGPQFVIMMNADTGKVIQSYPISAGVDANAF
                     DPGTNLLFVATREGMMHVYHEDSPEKLSEVGAVKTEYGAKTLQVDPKTHNVFLSTSDF
                     NPPAAPTEKQPHPLATPKAGNFRVLVFGK"
     gene            complement(7290..8273)
                     /locus_tag="VG272_11835"
     CDS             complement(7290..8273)
                     /locus_tag="VG272_11835"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWF14421.1"
                     /translation="MASRHIPIFFFGIVAAIFAAGCHHQTADSGLTKVVLQADWYPQP
                     EHGGFFTALAKGFYKDEGIDVTILPGGPYITVEQQVSAGAAQFGMGSSDKVLEADAGG
                     QSLVAVAATMQHDPQGIMVRKDSAIHSFSDLNGHSVAIKVGESTWFEFITKRYQLNNV
                     RVVPALMNVANFVADPQYIQQAFATSEPYFAHQAGVETRVLLVSDAGFNPYRVMYTTR
                     DFVQQRPDIVAKFVRASVKGWKEYLNDPAPANMVIAKLNPALNPDWMRFTWQQLKDGQ
                     FIAGEDPTGAQIGQMDPKRWATLYDQLVELKVIGKPIDPATAYTLQFIQNK"
     gene            8407..8949
                     /locus_tag="VG272_11840"
     CDS             8407..8949
                     /locus_tag="VG272_11840"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF012601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HWF14422.1"
                     /translation="MPWTGVSRSRGAAVDAAHMREIYEFTARSLETERPVPFGALIVD
                     SRTGERLMRATNAVFRENDPSAHAETRTVRLACKKLKRPSLAGYTMYSTCEPCAMCMA
                     NALWAKLDRVIFGATIADANRFCLQIHIPAKEVSRRSDMPCKVEGPVLRELCISLFTH
                     PNMQKAFRNWSTSKTRVAKT"
     gene            8974..10350
                     /locus_tag="VG272_11845"
     CDS             8974..10350
                     /locus_tag="VG272_11845"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HWF14423.1"
                     /translation="MDSKQIPEIRSLLADPARVIVNSQVVEQLSKDFYWYSPILRKQL
                     DGKIGELVVQPQRAEEIQKLLRYCHASSIPITARGAGTGNYGQAVPLEGGVILDLSRM
                     DRIEQIQPGGVAVCQPGVRLGDLENEARKSGWELRCYPSTIIKASIGGFVSGGSGGIG
                     TVAHGGLRDFQTVRALEVVTMEPEPRIVLHEGEAVHEILHAWGTNGIITRIWLALTPA
                     VEWAQCIAAFDTFAAAFDFAEKIATGAEWTKRLVTVFEWPIPSFFAPIRQHAPEGKTL
                     IFFMIASSQLSRLEIAVKGTRGIVTHSGIYPGLNARPLLSDYTWNHTTLWAMRSDEAY
                     TYLQCGFNPDTVRDQMRQLRERFGQEILFHMEFMKNGDGRVVPGAIPVVLFKTEERLN
                     DMIDFCRRIGVFVANPHVNNVEGGGRYRADNVQLLAKLRYDPKGLLNPGKMITFHTES
                     ATAGANSK"
     gene            10347..11141
                     /locus_tag="VG272_11850"
     CDS             10347..11141
                     /locus_tag="VG272_11850"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HWF14424.1"
                     /translation="MKTWIPDARNFAYLNWKQVDALPRGTTLLVLPTAAIEQHGHHLP
                     IATDTLINNLLLGHALRKLPAELTVYALPPVHYGKSNEHIGFPGTLSVSSATFMAVLR
                     DLGSSLAKVGFQKLVLYNTHGGNTALIDVMARDLRAEFGLRTFAMHGSGGVSFEGLDP
                     QERAYGFHAGEVETSFLLTSVPDLVNQSAYTSNYIADITKPAMLLPENAPATFAWLTK
                     DIAASGVMGDPRPATAEKGARWLEQAATRIAAALTEMYHYTDLSTP"
     gene            11138..12853
                     /locus_tag="VG272_11855"
     CDS             11138..12853
                     /locus_tag="VG272_11855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HWF14425.1"
                     /translation="MSPPGCVDCGDGVRLERGVRARMRDGVELISDHYYSPQPGPHPT
                     ILMRQPYGREIASTVVYAHPVWFARHGYNVVIQDVRGRGDSEGEFYPFRNEGADGADT
                     IAWLRARPESNGRVGMYGFSYQGITQLLAAAEQPEGLQCISPGMAACDLYHGWFYQGG
                     ALRLASTLGWGLQLLKEDARRKKLREASEMLEHAWAHLAAQTSVLPVRAHPALHSEGL
                     PQYVLDWFDHNAPGDYWGALDVSRRLNKILIPALHVSGWYDTFLRGSVDGFVALSNCA
                     GTEFARRNQFFLAGPWLHIPWGDRIGAADFGPEALIDTDAMLLRWFNHWLKDSGSFAN
                     EARIRHFVLGENRWREADRFSTGADRAFFLRSEGRANSRKGNGVLSTTAPTAKEPCDI
                     FVYDPEVPVIAPGGPAAASGQFDQAALELGNNLLVYSSAPLEEPLRIFGKPRAVLHCS
                     TSAISADFTAKLVRVRANGAADFICIGIARSTHLFAGSKYEADKIHRWEISLDPTSCR
                     FERGECVRLEIAGSAFPLLDRNSGSDVPSCEATSWDWRRSTHMVFHSDESPSALYLPL
                     AEDPQ"
     gene            12850..13623
                     /locus_tag="VG272_11860"
     CDS             12850..13623
                     /locus_tag="VG272_11860"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWF14426.1"
                     /translation="MSANGDVPEIEIVGVTKRYGDARAVLESINVVIEKQEFVSIIGP
                     SGCGKSTLLKLIAGLTSPSSGTICVDGMTPLNARETVSFVFQDATLLPWRTVKENICL
                     GMELERVPKGRREKEIAMLLELVGLKPVSGAHPRELSGGMKMRVSIARALATHPRVML
                     LDEPFAALDEMTRDRLNEEILRLRAEQRWTAVFVTHSVPEAVFLSDRILVLAPNPGRV
                     HAEFRVDLPMPRTSAVRRSKEFDALTSLVAHTLRETIPQ"
     gene            13620..14411
                     /locus_tag="VG272_11865"
     CDS             13620..14411
                     /locus_tag="VG272_11865"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWF14427.1"
                     /translation="MKQKTITVLNALAVFAAFLFLWQIVLWIFHVPRFMLPSPYAVAL
                     AVVVRFPSLMNALAITAEESAGGLIASIVAGVLVALVFAQSRWVRKMLYPYTLLLQTV
                     PIIAIAPLILMWMGAGIPAVTLIAFIISLAPIIANTTQGLISVDENLVHLFLMHNASS
                     RQILFKLRLPNAIPNLFAGMRIASGIAVIGAITGELFAGSGKVGEGGLGYSILYASSQ
                     LQTEYLFALVLAATVLGFGFLFLIMFLEWYFLHKWHESARVTETE"
     gene            14454..15278
                     /locus_tag="VG272_11870"
     CDS             14454..15278
                     /locus_tag="VG272_11870"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HWF14428.1"
                     /translation="MLRLESETGWWLVTHPNHARLAGAFAERWGNDLFLPPEPREHVM
                     RGIARHDDGWVARDAEPQITMQGKPSAFSTELVGKYSAFEEIDLADYLAVRDRAVRLI
                     AAEDPYAAILISMHTYSLLHDHADRSTIAAAQLPLLDQFLEDQTSLQGSLRKQVAANP
                     KLQPDQKSDDAILDHFHLLQANDNLSLLTCVDFQQPANLLHALPTRDGRRIPVEVRSV
                     GIRRFVLKPYPFAERTLTFQFPARHAKGKQFSTAKELQDAYNAAPKEMLSVMVSAE"
     gene            complement(15320..>15917)
                     /locus_tag="VG272_11875"
     CDS             complement(15320..>15917)
                     /locus_tag="VG272_11875"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HWF14429.1"
                     /translation="ADPLIPTSGPRGPVLKAGIVILLVLAGFLAGLPPSQVAAVGAAI
                     MLMTRRHEPRRVYDEVDWGLLVFFVGLFLIVGGAENVGLTARLMEVGKQWNLEHAHTL
                     TMVTAALSNVVSNVPAVMLLKSVVPRFANPQRGWLVLAMASTLAGNLTITGSVANIIV
                     VERAKPEVNITFGQYLRTGVPLTLLTLAFGWLWLLWTS"
BASE COUNT         3601 a         4345 c         4404 g         3567 t
ORIGIN      
        1 gacgtgaatt tccagatttc agaaggggaa cgatacacgg tcgctgattt gaaagtcgat
       61 gatcatgatc acaatacaac gggaactatc ggcgatcgcc acttcctgaa cctattcccc
      121 ggcaagccgt tctctccggc gttgctgcaa caggacagga acacaattct cgcgggttat
      181 ctgaaccgtg gatatgaaaa cgcccaattt cattccacgt tcaccgctga accaaccaat
      241 gcgcacctga tcgatgtggt ttacacgatt gatcccggcc ctcaggccag aatcagcgat
      301 acagttgcac tcggaactga agtgactagt caaagtttcc ttcatgacat tacggggccg
      361 gagatccgca gaggcaagcc catcagcctg ggaaattttc tgacatctga aagcgactta
      421 tacaatctga gcattttcga ttgggcgagc atcaaacctc tcgcgccggt aagcgatcag
      481 gaacaggaag aggtgctggt taaggttcac gaagcgccgc gaaactcggt cgatttcggc
      541 ggagggtttg aagtgcttcc gcgcagcgga aatattccag tagggactgt cgcgctgccc
      601 ggcattcctc ctattggagt gggcagccgt ttcactgcca gccaacagag tttctgggga
      661 cctcgcgttt ccattcaatt tgcccgccac aatctgcgtg ggcgggcgga gaccgcgacg
      721 ctgggcttgg tctattcgcg tttggatcag agaggaactt tcacatatac cgatccacgg
      781 gtgcgcggct cttcctggaa ttcgcttttc agtttgtcag cggagcgcac cacgcaaagt
      841 tccatctaca cgggcgaatt ggcgcaggcc tccctgcagg tcgaacgttt cttcgataaa
      901 aagaaaacta aaaatttaat tttgcgatac acctatcaac gcacgctgct gaccaatatt
      961 acgattcccg acctggtttt gccggaggac caacatgtgc ggctttccac ggtagccgct
     1021 cagtacgtac atgacacgcg cgacaagccg cttgatgcgc atcacgggat ctatcaaacc
     1081 gcaacgctgg agatttcgcc cacgaaactg ggctccagcg taaattttct gcgatttttc
     1141 ggtcagactt ccttctacaa gccgctgact ccctggctca cgtgggccaa taattttcgc
     1201 ctcggtcttg cgaaaccgtt ggcgggcggg acgattcctc tcagtgaacg atttttttcc
     1261 ggtggcgcgg attccattcg cggatttccc atcaacggag cggggccgca gcgcccggtc
     1321 tccgtgtgca gtaatccaag cgacgcttca acgtgcaccc tgatcagcgt gccggttggt
     1381 ggaaatatgc tggcagtggt taactccgag gcgcgttttc ccatcccgtt gaaaagcggg
     1441 ctgggtggcg tagttttcta cgacggcggg aacgtttaca acaggattaa tttgcgagag
     1501 tttgcagaca actttacgca cacggtcggt tttggcttcc gctatgaaac gcctgtaggc
     1561 ccggtgcgat tcgatatcgg gcggagactg acctccgtgc ccggagtgac cgcgacggaa
     1621 tattttgtga cgctggggca atcattttga ggcgggggtc gacaggattt ggcagggacc
     1681 gggtgaaaca atgagcggga aacgcgttgt tcgatggacg gcaattgtgc tcggcgggct
     1741 acttgcggcg ctggtgatag cggcaatcgt ggtggtgcac acaaccgcat tccagcgttt
     1801 tgttgtgtcc cagttggagc aaaaagtgca agccgggacc ggcgcgcgcc tcgacataaa
     1861 aggattgagc atcaactggc gattgttaac gctggattta tatggcatca ctctgcacgg
     1921 gagagaaacg gatccgcaac cgccgctact tgccgcggac cacctgcgcg tggggctgaa
     1981 aattatttcg ctcttgaggc aaaaagtgga cttgcgcgaa attactctgg atcatccggc
     2041 cgctcatttc acggttgatc gcagcggagt ttccaatctg ccgcaaacga attcctccgg
     2101 taccacgtca tctgctgtgg acagcattct gcagctcacc atcggccacc tgcagctgaa
     2161 ctcgggagtg ctttattaca ataatgcgca gattccgctt aacgccgacg tgcgggactt
     2221 gcgcgcggac gtccattaca atccgattag cagtgagtat gccggtacgg ttggatacaa
     2281 cgacggccgc attgcgaccg gagggttgaa acaagtccct caccgcctgc agctgaattt
     2341 ctcgctcgat cgctccggta tcgtcgcgag tcccttcacc attgttaccg cacaatccac
     2401 actgaacgtg cgtgcaaaac tcaccaacta tgatcacccg cagttggatg gaacatacga
     2461 tgcggcactg tccactcaag agcttgcgcg atacctgaac aacgcatcgc tccccgccgg
     2521 cgacgttgca ttaagtggtc gcttgcattt cgaaaacgtg gcgaataaat ccgcactgga
     2581 aagcgcgtat gttgacggcc agctaaaaag ccagcaattg ttggtggaca tagggcagat
     2641 gcgagcgatt ccgaaatcca tccacgccga attcaagttg caggacggaa acctaagcgt
     2701 gaacaaggta gaagcggatc tgcttggcgg ccacgtcagc ggggattacc agttatcgca
     2761 cctcagcgga aattccagtt cacatgcccg tgcgctgctt cgcgacgtgt cgcttcagca
     2821 gttggatagg acggcgaaaa tacaaaaagg gaagcctttg aaaatcggcg ggcgcgtgaa
     2881 tggtactgct gaggcgatgt ggtcttctag cgttcaaaat gcagttgtaa aggcgaattt
     2941 cgatattcgc agcccaaatt cgtcgagtgc cctagagggc gttattcctt tgagcggcct
     3001 gattcatgtt gcttatgacg gcgcgcaaaa tagcgcgtcg ttcgggcagt cgtatctgca
     3061 aacggggaac acaaaaatta cggtatctgg cgttttgagc agacactccc ggttaagtgt
     3121 gcaggcaaac accacggatg tgcatgaagt tgccgcgctt gtttcgttcg tgcaagccgg
     3181 gaactccgcg aatgcgactg gcgctggaag ttcattgccc gacgtaggag gcgccgctcg
     3241 atttatgggt gaggtctcag gcgccattca gcaaccgcga atccgaggcg atatctcggc
     3301 gagtggcctg caagtgaaga atgccaagtg gcgaacattc cacgcgacac ttgacgcgtc
     3361 cgcatccggc gttacttttc aaaatgcatc cctcgtagat ttgcaaaatg ggcaaatcaa
     3421 cttcagtggg catgctgatt tgtcggcatg gtcgttcacc cctgccagtc cgctcacctt
     3481 gcaactcagc gcgagacaga tttcgatttc agagttgcag gaattcttcc aatcgcaata
     3541 tccggtgtcg ggagtcgtcg cggctaattt atcgattcac ggcagcgagc agaatcctga
     3601 ggggcaggga tcgctgcaga tcaccaaagc gactgcgtgg aacgaaccca ttaaggattt
     3661 gagcgtaaat tttcgaggcg acggaaacac tgttcacacc gatgcccaac ttgcgattgc
     3721 cgccgggaat ctcgccgcga ctttgacgta ccagcccaaa acgcaaacgt acgacaccaa
     3781 actaaccacg gcgggattga agctcgacca actcgaatcc gtgcaggcgc gcaatcttgg
     3841 tgtcgcgggc gatctggcgc tgtccgcaca gggaagtgga acttttcaga gccctcagtt
     3901 cacggctgat ttaaaaattc ccaaactcgc cattcggaat caaactgttt ccgacgtgca
     3961 ggcgcaactt agcctcgcaa atcagcgcgc cacgttcact gtgaattcgc gggcggacca
     4021 gggaacgatt caggcaaaag gaaatgtggg actttccgga gattatccgg ccacagcatc
     4081 cattgatatt cgtgcgatcc cggttgccct ggtactggcg cgctatttga caaaaacgca
     4141 ggatattcag gggcaaacgg aaattcatct caatttggac ggaccgctga aaaatcctta
     4201 cgcgatcatt gcccagctgg aaattccaac gctcaacgtg aattacgaaa ccgccaaaat
     4261 tggactcgtc cgtcctctga aggcttcgta tcgaaatggc gttgttaccg tcgatcaggc
     4321 ggagctgaag ggaacgggaa cggatttgaa tttaaaaggt gtgattccga tcaaatcctc
     4381 ggcgccactg aatgtttctg ccgccgggtc tctagattta tctgttctgc aggcattcac
     4441 caccggaacg aaaagttccg ggaacatcaa cctcgatatt tccgcgagcg gcgatttctt
     4501 gcatcccgcg atgcggggca atataaaaat tgtaaacgcg gcgattgcca cggaaacctt
     4561 gcctgtgagt atcgaaagat tgaacggaga aattcaagtg tcaggaaacc ggttggaagt
     4621 ctctcagcta tctggcgcag cgggcggcgg cacagttgcc gtcaggggtt ttatgaccta
     4681 cggcagccaa tccaacttca atgtgggcct ggatgcgaat aatgtacgca tcagatatcc
     4741 ggagggaatt cgttccgttt taagcggcaa tctgagcgtc gccggaagtc cggcgagttc
     4801 gcagctttcc ggacgcgtct tgatcgaccg gctttcgttc acgcagcaat ttgatctggc
     4861 tactctgctt ggcggttttt caagcgattc gcccagcaca acttcttcac cgcttgagag
     4921 caacatgaaa ctgaatgtag ccgtggccac cgcgcaggat atcaatctca ccagcagtaa
     4981 agtcagcatg ggtggcgctg cgaatttgac tctgtcgggc acggttgcaa atccggtcgt
     5041 gcttggacgc gcgacgctga cgagcggcga aatgtttttc atgggcaagc gctacgaaat
     5101 tcaaaacggc acgattgagt ttgcaaatcc tgttcgcacc atgccggtag tgaatttgta
     5161 tgtaaaaaca accgttcagc aatacaacat cacactgaac ttcgttggcc cggtggacaa
     5221 gttgcgcacg aactacacgt ccgatcctcc gctgcctccc gccgacatta taaatcttgt
     5281 cgcgtttggc aagacggcgg aagaggctgc cagcagttcc agcacaccag cctcgctagg
     5341 cgcagagtcc gtgctcgcgc aaggggcggc gagccagttg agcgggaaat tagaaaagct
     5401 ggccggaatt tcacagttga ccattgaccc ccttgccgga aacagccaat ctaatcccgg
     5461 cgcacaagtg gccattcaac aaagagtcac cggaaaaatt ctgcttacct tcagcacgga
     5521 tgtcacgtcc acgcaaaatc aggccatcca ggttcaatac cagataaaga agggcctgtc
     5581 tgtttccgtt ttgcgcgatc aatatggcgg gtatgcggcg gacgtgcgaa ttcacaaaac
     5641 tttctgaact gcgatcctcc tgaagcttgc ctcgccgtac gcacagctac agataaaacg
     5701 tgcccgcgag tcctcatcag cctaaacgat taacggcatc cgcctctact tggttgcctc
     5761 tacagcgaag ctgccccaaa aaaaccagcg gaaatctggc aggcaatgat cacctgtgaa
     5821 gtaaccccgt tgagctacac tgtgcaaaca gcaattcatt caggagttat tcaggaacgt
     5881 cctataggct ataggtcacc acgcctctga ggtcgatgct acaattgcgc aattcgtggg
     5941 accgaccgag ggtccgcgga gcacatgggt gcaacggtgc cggcaacgat ctagctgaca
     6001 ctaattgatt gcggcaccga atgacgatga acttctgccg ccggtgattc tgacgtgcgg
     6061 tgcggtaggt tcgtgttttt gaaggtttcg atagcacaaa tacaaaggag cgttgatatg
     6121 aagctttcca cgattggccg cctcgccgcg ggtctgagcc ttacggttgc aggtgcatgc
     6181 ctgttgtcgg gaacacatgc tcagcccaaa cctgcggact ctggctacca tttgctgaag
     6241 acgatttcgc tgccagcggc gccgggcgga gcggaatatt acgactacat tacgatcgac
     6301 gcggagtcac gccgcgttta catttcccat ggtacggaag ttgtagtgtt gaatgcggac
     6361 gattattccg tagcgggtaa gatcgaaggg ctttcacgca gccacggaat tgcgattgcc
     6421 aaggacctgg gcaagggctt cattaccgat ggagactcgc gggacggcgc tacagttcag
     6481 gaagccgtcg tttttgattt gaagaccttg aaagtaacaa accatgtaaa gaccggtcag
     6541 ttggacaccg atgcgattat ctacgaacct gtgaacaaaa agattttcac tttcaacgga
     6601 gatagccaca acacggtggt aatcgatgcc gtgaaagaat cggtgatcac gacgattgat
     6661 ctgattggca aagtggaatt tcccgcggtg gatggtaaag gtctggtgtt tgacaacaat
     6721 ccggaaaaga acgatatcgt tgtaattgac gcgaagacga ataccattaa gacgcgttat
     6781 ccgaccgctc cggaagggaa tcccacctcc atggcgattg atcaaaagac tcgccggctt
     6841 ttctccggtg gtcgtgggcc gcaattcgtg atcatgatga atgccgatac cggcaaagtg
     6901 atccagtcct atcccatctc cgcgggcgtc gacgccaatg cgtttgatcc ggggactaac
     6961 ctactgttcg tcgccacgcg cgaaggcatg atgcacgtgt accacgaaga ttcaccagaa
     7021 aagttgagcg aggtcggagc cgtaaagaca gaatacggcg ccaaaacttt gcaggtggat
     7081 ccaaaaacgc ataatgtttt cctgtccact tccgatttca atcccccagc cgcgccgacg
     7141 gagaaacagc cccatccgct cgcaacgcca aaggccggaa acttcagagt cctggttttt
     7201 gggaagtaag tttctcagac tcgaaattca aaaaagcgcg gttcgccgga aatcggcgag
     7261 ccgcgctttt aatttttagc tcgcgcgatt tacttgttct ggataaattg cagtgtgtaa
     7321 gccgtggctg gatcgatggg cttaccgatg actttcaatt cgacgagttg gtcgtacagc
     7381 gtggcccacc gtttgggatc catttggccg atttgcgctc cggtcggatc ttctcccgcg
     7441 atgaactggc catccttcaa ttgctgccac gtaaatcgca tccagtccgg attgagcgct
     7501 ggatttaatt ttgcgatcac catattggct ggcgcaggat cgttgagata ttccttccaa
     7561 cctttcacgg atgcgcgaac aaacttcgcg acaatatcag gacgctgctg cacaaagtct
     7621 cttgtggtgt acatcacacg gtagggatta aatccggcat cgcttaccag aaggacgcgc
     7681 gtttccacgc cggcctggtg cgcaaaatac ggttcggatg tggcaaaagc ctgctggatg
     7741 tattgcggat ccgctacaaa gttggccacg ttcatcagtg cgggaactac gcgaacgtta
     7801 ttcagttgat agcgcttggt aataaactca aaccatgtgg attcgccaac ttttatggcg
     7861 acagagtggc cgttcagatc cgaaaaggaa tggatggctg aatcttttct gaccatgatt
     7921 ccttgtggat catgctgcat cgtagcggct acggcgacca gtgattgtcc tccggcgtcg
     7981 gcctccagaa ctttgtcaga cgaccccatt ccaaattgcg cggcgcccgc ggagacttgc
     8041 tgttcaacgg taatatacgg cccgccgggg agaatggtga cgtcgattcc ttcgtcttta
     8101 taaaaaccct ttgctaatgc ggtaaaaaag ccgccgtgct cgggttgcgg ataccaatcg
     8161 gcctgcagga ctaccttggt caaccctgag tcggcggtct ggtgatggca gccagccgca
     8221 aaaattgccg ctacgatgcc gaagaaaaaa atggggatat gacgactggc cataggcggc
     8281 agactcattc gtgtgcaacg gcgagaatac cacgctgctg cgcccgaaaa accgcttaat
     8341 cgtttccatg caaaaattga atagacgtcc gcaccggtcc aggcttccgg tgtggtagat
     8401 tgcggcatgc catggacagg ggtttcacga tcccgcggcg cagctgtgga cgcagcgcac
     8461 atgcgcgaga tttatgaatt tacggcgcgc tctctggaga ccgagcgccc cgtcccgttc
     8521 ggcgcgctga tcgtagattc gcgcacgggc gaacgtctga tgcgcgcaac gaatgctgtg
     8581 tttcgcgaaa acgatccgag cgcccacgcc gaaacccgaa ctgtccggct cgcctgcaaa
     8641 aaactgaaac gaccatctct tgccggctac acgatgtatt ccacctgcga gccctgcgcg
     8701 atgtgcatgg cgaacgcttt gtgggccaag ctcgaccgcg tgattttcgg tgcgaccatt
     8761 gccgacgcca accgcttttg cctgcaaatc catatacccg caaaggaagt ctcgcgccgg
     8821 tccgatatgc cgtgcaaggt ggaaggcccg gtcctgcgtg agctgtgcat ttcacttttc
     8881 acgcatccga atatgcagaa agcatttcgc aactggagca ccagcaaaac ccgggtggct
     8941 aaaacatagc ttggaatcgg aagcagacta tccatggact ccaaacagat tcctgaaatc
     9001 cgttcactac tcgccgaccc tgcgcgagtg attgttaatt cccaggtagt ggaacaactc
     9061 tccaaggatt tttattggta ttcgccaatt ttgaggaagc agctcgacgg gaaaatcggc
     9121 gaattggtgg tccagccgca acgtgccgaa gaaattcaaa agcttttgcg ctactgccat
     9181 gcgagcagta ttccgattac cgcgcgcgga gccggaacag gcaattacgg gcaggcggtt
     9241 ccgctcgaag gcggcgttat cctcgatctg agccgcatgg atcgcatcga acaaattcag
     9301 ccgggtggcg tggcggtgtg ccagccagga gtgcgcctcg gcgacttgga aaacgaagcg
     9361 cgcaaatccg gctgggagtt gcgttgctat ccttccacta tcatcaaagc atccatcggc
     9421 ggatttgtta gcggcggttc tggtggaatc ggcacggtag cccatggcgg cctgcgcgat
     9481 tttcaaactg tgcgcgctct cgaagtggtg accatggagc ctgaacctcg catcgtcttg
     9541 catgaaggcg aggcggtgca cgaaattctt cacgcctggg gtacgaatgg cattatcacg
     9601 cgcatctggc tggcgctcac gcctgccgtg gagtgggcgc agtgcatcgc ggcgttcgat
     9661 acttttgctg ccgcgttcga ttttgctgag aaaatagcaa cgggtgcgga atggacgaaa
     9721 cgccttgtca cggtattcga gtggcccatt ccttcttttt ttgcgccgat tcgccagcat
     9781 gcgccggaag gcaagacgtt gatttttttc atgatcgctt cgagccaact cagccgactg
     9841 gaaatcgcag taaaaggcac gcgtggaatc gtcacgcaca gcggaattta tcccggcctc
     9901 aatgcgcggc ctttgctttc ggattacacc tggaatcaca ccacactgtg ggcgatgcgc
     9961 tcggatgagg cctacaccta cttgcaatgc ggctttaatc ccgatactgt gcgcgatcag
    10021 atgcggcaat tgcgcgagcg cttcggccag gaaatcttat tccatatgga gtttatgaaa
    10081 aatggcgacg gccgcgtcgt tcccggtgcg attccggttg tgctatttaa aacggaggaa
    10141 cgcctgaacg acatgatcga tttctgccgc agaataggag tgttcgtagc caacccccat
    10201 gtgaataacg tggaaggcgg cggccgctat cgcgccgata atgtgcagtt gctggctaag
    10261 ctgcggtacg atcccaaagg tttgctcaat cctggcaaga tgattacttt tcatacggaa
    10321 tccgcgacgg ccggagccaa ctcgaaatga aaacctggat accggatgcg cgaaattttg
    10381 cgtacctgaa ttggaagcag gtggatgccc tgccgcgcgg cactacgctt ctagtgctgc
    10441 ctaccgcggc gatcgagcag cacggccatc acctgccgat tgcgaccgac actctgatca
    10501 acaatctgct actcgggcat gcattacgaa aattgcccgc ggaattaaca gtctacgcgt
    10561 tgccgccggt gcattacggc aaaagcaatg aacacattgg ttttcccgga acgctttccg
    10621 taagttccgc caccttcatg gccgttttgc gtgaccttgg ctcgagcctg gcaaaagtgg
    10681 gcttccaaaa actcgttctc tataacaccc acggcggcaa tactgccttg atcgatgtga
    10741 tggctcgcga tttgcgcgcg gaatttgggt tacgcacatt tgctatgcat ggctcgggcg
    10801 gagtttcgtt cgaggggctc gatcctcagg aacgcgccta cggttttcac gccggcgaag
    10861 tcgaaacttc ttttcttctg acgagcgtgc cggacctcgt gaatcaatcc gcgtatacat
    10921 ccaattacat tgcggatatc acgaaacccg cgatgctgct gccggaaaat gctcctgcaa
    10981 cttttgcatg gctcacgaag gatatcgcgg ccagcggagt gatgggcgat ccgcgccccg
    11041 cgaccgccga aaaaggcgcg cgctggctgg agcaggcggc aacacggatc gccgccgcgc
    11101 tcaccgaaat gtatcactac acggatttgt ccacgccatg agcccacccg gctgtgtgga
    11161 ttgcggcgat ggcgtgcgtc tcgagcgcgg cgttcgcgcc cgcatgcgcg acggcgtcga
    11221 attaatttca gatcactatt attctccgca gcccggcccg catcccacaa tcttgatgcg
    11281 ccagccatac gggcgggaaa tcgcatcaac ggttgtatat gcgcatcccg tttggttcgc
    11341 gcgccacgga tataacgtcg tcattcagga tgttcgcggg cgcggcgatt ccgagggcga
    11401 attttatcca tttcgaaatg aaggggctga cggcgccgac acgattgcct ggttgcgcgc
    11461 gcggcccgaa tcgaacgggc gcgttggtat gtacggattt tcgtaccagg gcatcacgca
    11521 actgctcgcc gcagcggaac aacccgaagg tttgcaatgc atttcccccg gcatggcggc
    11581 gtgcgatttg tatcacggtt ggttttatca gggcggcgcg ctgcgcttgg cgtccacgct
    11641 gggctgggga ttgcagcttt tgaaggaaga cgcacggcgc aagaaactgc gggaggcgag
    11701 cgaaatgctc gaacatgcgt gggcgcatct cgccgcgcaa acaagcgtgc tgccggttcg
    11761 tgcgcatccc gcgctgcata gcgaaggcct gccgcaatat gtgctggact ggttcgatca
    11821 caatgcgcct ggcgattact ggggcgcgtt ggacgtcagc cgcaggctga ataaaatttt
    11881 aattcctgct ttgcacgttt ccggatggta tgacacgttt cttcgcggca gcgtcgatgg
    11941 gttcgtcgcg ctttccaatt gcgcgggcac ggaatttgcg agacgaaatc aattctttct
    12001 cgccggtccg tggctacata ttccttgggg cgaccggatt ggcgctgcgg attttggccc
    12061 tgaagcgctc atcgatacgg acgcgatgct gcttcgctgg ttcaatcatt ggctgaaaga
    12121 ttccggctcg ttcgcaaacg aggcgcgcat ccggcatttt gtgctcggcg aaaatcgctg
    12181 gcgcgaagcg gatcgttttt ccactggcgc ggatcgcgca ttttttctgc gtagcgaagg
    12241 tcgggctaat tcgcgcaagg gaaatggtgt cctctcgaca acagcgccga ctgcaaagga
    12301 gccatgcgat atttttgtgt acgatcccga agtgcccgtc atcgctcccg gcggacctgc
    12361 tgctgccagc ggtcaatttg accaggcagc tttggaattg ggaaacaact tgctcgtgta
    12421 ttcgtcagcg ccgctcgaag agccattgcg gattttcggc aaaccgcgcg ctgttctcca
    12481 ctgctcgact tctgcaatta gcgcggattt caccgcaaag ctggtgcgtg ttcgcgcgaa
    12541 tggcgccgct gattttatct gcattggaat tgcgcgctcg acgcatctct ttgcgggatc
    12601 gaaatatgaa gcggacaaaa tccaccgctg ggaaatttca ctggacccta cctcttgccg
    12661 cttcgagaga ggagaatgcg tccgtctgga aatcgccggc agcgcgttcc cgcttctaga
    12721 cagaaactcc ggaagcgatg ttccctcctg tgaggcgaca tcgtgggact ggcggcgatc
    12781 aacccacatg gtgttccact cagacgagtc tccttccgcg ctttacctcc cacttgctga
    12841 ggacccgcaa tgagcgcaaa tggcgacgtt cccgagatcg aaattgtggg cgtcacgaag
    12901 cgatatggcg acgctcgtgc tgtgttggag tcgatcaacg tggtcattga gaagcaggaa
    12961 ttcgttagca tcatcgggcc ttcgggctgc ggaaaatcta cacttctcaa attgattgcc
    13021 ggacttacct caccctcgag cggaacgatt tgcgtggatg gaatgacgcc gctgaatgcg
    13081 agggagacag tctcgttcgt atttcaggac gcgacgctgc tgccttggcg aaccgtaaaa
    13141 gaaaatatat gcctcggaat ggaactcgag cgcgtgccga agggccgccg cgaaaaagaa
    13201 atcgctatgc tgctcgaact tgtcggattg aaacctgtgt ccggcgccca cccgcgagag
    13261 ctgtccggcg gaatgaaaat gcgtgtctcg atcgcgcgcg ccctggcaac gcatccgcgc
    13321 gtcatgctcc tcgacgaacc gtttgccgcg ctcgatgaaa tgactcgcga ccgcctgaac
    13381 gaagaaatcc tgcgcctgcg cgccgagcag cggtggaccg cggtgttcgt gacgcactcc
    13441 gtcccggaag ccgtttttct ctccgaccgt attctggtgc tggcgccgaa ccccggtcgc
    13501 gtgcacgcgg aattccgcgt ggatttgccg atgccgcgaa cgtctgcagt ccgcagatct
    13561 aaagaatttg acgcactcac gtctcttgtg gcgcacacat tgcgggagac gatcccgcaa
    13621 tgaagcagaa aaccattaca gttttgaatg cgctcgccgt tttcgccgcg tttctgttcc
    13681 tgtggcaaat cgtcttgtgg atcttccacg taccgcgttt catgctgcct tcgccgtacg
    13741 cggttgcgct cgccgtcgtc gtgcgatttc cttccttgat gaacgcactg gcaattacag
    13801 cggaagaatc cgcgggcggt ctcatcgcaa gcatcgtagc gggtgttttg gtggcgctcg
    13861 ttttcgcgca atcgcgatgg gttcgaaaaa tgctttatcc ctacacgctg ctattgcaaa
    13921 ccgtgcccat cattgcgatc gcgcctctta tcctgatgtg gatgggagcg ggaattcccg
    13981 cagtcacgct cattgcgttt atcatttcgc tcgcgccaat tatcgcgaac accacgcagg
    14041 gcctgatcag cgtggacgaa aatctggtgc atctttttct gatgcacaac gcttcaagca
    14101 gacaaatcct tttcaagttg cgcctgccga atgctattcc caatcttttc gcaggcatgc
    14161 gcatcgccag cgggattgcg gtgattggcg cgattaccgg agaattgttt gcgggctcag
    14221 ggaaggtcgg tgaaggcggt ctgggctact cgatactcta tgcgtcctcg cagctgcaga
    14281 ccgaatattt atttgcgttg gtcttggccg cgactgtact tggctttggc tttcttttct
    14341 taatcatgtt tctggaatgg tatttcctgc acaaatggca tgaatcggcc cgtgtcaccg
    14401 aaacggaatg accgggtaat gcaaagccat aaattcctca cgggagaaga caattgctgc
    14461 gactcgaaag cgaaaccggt tggtggctcg tgacgcatcc taatcatgca cgcctggcgg
    14521 gagcatttgc cgagcggtgg ggcaacgatc tttttttgcc gcctgaaccg cgcgagcacg
    14581 tcatgcgcgg gatcgcgcgc cacgatgacg gctgggtcgc gcgcgacgct gagccgcaga
    14641 tcacgatgca aggcaagccc agcgcctttt ccacggaact cgtgggcaaa tattcggcgt
    14701 tcgaggaaat cgatctagcg gattacctgg cggtgcggga tcgcgccgtt cgcctcatcg
    14761 ctgcggagga tccgtacgcc gcgatcctca tttccatgca cacctatagc ctgttgcacg
    14821 atcatgcgga ccgctccacc atcgcggccg cgcaattgcc gctgctcgat caattcctcg
    14881 aagatcagac atcgctacag gggtctctgc gaaaacaagt cgccgcgaat cctaaactcc
    14941 agccggatca aaaaagcgat gacgcaattc tcgaccattt tcatctgttg caagcgaatg
    15001 acaatctttc gctgctcacc tgcgtggatt tccaacagcc ggcaaattta ttgcacgctc
    15061 tgccgactcg cgatggccgg cgcatcccgg ttgaagtgcg ctcggtgggc attcggcgat
    15121 ttgttctgaa gccgtatccg tttgcggagc gcacgctgac gtttcaattt cccgcgcggc
    15181 atgcaaaagg aaagcagttt tcaacagcta aggaattaca ggatgcgtac aacgcagcgc
    15241 caaaggaaat gctttcggtc atggtcagcg cggaataaat gcaatgagag ttgaagaatt
    15301 ctgggtagtg gaagtgcgct caactggtcc acagaagcca aagccagcca aatgccagcg
    15361 ttaaaagcgt aagtggcact ccggtccgca aatattggcc aaacgtaatg ttcacttccg
    15421 gcttcgcgcg ctccacgaca ataatattgg ccactgaacc ggtgatcgta agattgccgg
    15481 ccagcgtgct ggccatggcc aataccaacc agcctcgctg cggattcgca aatcgcggca
    15541 ctacggattt cagcagcatt acagctggga cgttgctcac tacgttgctc agcgccgccg
    15601 tcaccattgt caaagtgtgc gcgtgctcca gattccactg tttgccaact tccatcaggc
    15661 gagcggtcag gcctacattt tctgcaccgc ccacaatcaa aaataatcct acgaaaaaaa
    15721 caagcagccc ccagtcgact tcgtcataga cccgccgcgg ttcgtgtcgg cgagtcatca
    15781 gcatgatagc cgcacccacc gcggcaactt gcgacggcgg caagcccgca agaaatcccg
    15841 caaggaccag caaaatcacg attccagctt tgagcaccgg tcctctcggg cccgacgtgg
    15901 gaatcagagg atcggca
//
LOCUS       DASZMB010000254        22639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2458725, whole genome shotgun sequence.
ACCESSION   DASZMB010000254 DASZMB010000000
VERSION     DASZMB010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 22639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22639
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2458725"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VG272_11880"
     CDS             <1..263
                     /locus_tag="VG272_11880"
                     /inference="COORDINATES: protein motif:HMM:NF023376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HWF14430.1"
                     /translation="DEKELVGQFSNLVMFHHTAEEFTVHFIYVFPNVPQGKLVSSVIV
                     SPGHAKRILRALEENIRRYEKQFGPIREVPDPTPVPNVGFVQ"
     gene            566..1132
                     /locus_tag="VG272_11885"
     CDS             566..1132
                     /locus_tag="VG272_11885"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWF14431.1"
                     /translation="MKAVCSSCGAQHSLPDKQLVGLPRVQFQCAKCGKNTVVEISASP
                     DATQILTPLPGFARSAGAPWLAGSSANEANSLQLPTGKAIALSVIAGPSRGLVYPIEK
                     PRVVLGRADADVVINDKEISRWHCSIEIKGDVIRLRDMDSTNGTYFADERVRVAELKH
                     LSEFRIGTSVILLSVTPKLAPTHATKIS"
     gene            1228..2016
                     /locus_tag="VG272_11890"
     CDS             1228..2016
                     /locus_tag="VG272_11890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWF14432.1"
                     /translation="MPRLTATLISYNEEVDLPRAFASLTGIADEIILVDSGSTDRTTE
                     VAQSFGARVYTRKLDSFATQKNYAASLSSNDWIFSMDCDEELSPELRASLLAWKEQTP
                     EKNGYEILLLTNYLGGWIRHSGWYPERKLLLYRRDKGQFESALHERVHLEGSPGRTQG
                     HLFHYTIRSLTEHRAKLDAMTTLAAEDMFARGYKIWRPAMIFAAPWTILQRLVFQGGI
                     LDGRRGWLIAWFSAKYIYVKYRKLGQLLGGKQLSHRSWPSPGGV"
     gene            2019..3116
                     /locus_tag="VG272_11895"
     CDS             2019..3116
                     /locus_tag="VG272_11895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWF14433.1"
                     /translation="MRPLLVDLGRDYRGGQDQALLLLKGLAALGHTPEFITIRGSLLA
                     ERAQSAGIHVHLVDKRWRRLSAVFAIRELLSRRVADIVHANEPHALTSAWLARAHHRV
                     PLVASRRVIFPLSKDAVSLARYRSAAKIIAVSQCVSSALINSGIQAGNITVISDGVPL
                     ENAFSQEDREQARRSLGIEPDALLIGCISALTPDKGHEILIRAFALIRARFPGCQLLL
                     VGDGPCRAKLAARVRELDLDGVVHFTGFVEDIQRVYAAIDLFAFLAQAEALGTALLLA
                     MAHGLPVVASARGGIPEVVQDGKNGLLLKDSSPEAVASAIERLISDSDSAARLGAAAR
                     ETILTHFSQEHMVRQTLSLYEQLVADRSSQM"
     gene            complement(3348..3680)
                     /locus_tag="VG272_11900"
     CDS             complement(3348..3680)
                     /locus_tag="VG272_11900"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWF14434.1"
                     /translation="MPATFDISRLEKRIPMTVAVHISERESSTCVETTFTEDVSSRGA
                     RVPSARRWNRNEILNFESLPGDFRALARVAYCRPHPDGYVIGLEFLEPNGRWVITAPV
                     GSGKPLQG"
     gene            complement(3729..5021)
                     /locus_tag="VG272_11905"
     CDS             complement(3729..5021)
                     /locus_tag="VG272_11905"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HWF14435.1"
                     /translation="MLSQIQVWSYRLRWFLGILGAAAFLIFVVWLRQLPFSSEPILYS
                     YFQIVGSLLCFTYAANAMVRFRGTRDRLTLILAFGFVLSGAIETAATFNFYDVQASDA
                     YSQLHVPLTWMVSRTLLALVLIAALFVERRVPNSREPSKEIAVALFVVVAVAYATSMA
                     YLWSPLAQVMHPRAIVSRTGDILPAVLFAVAAIGFTRRSRRNPSPFDSIFCIALWMNV
                     ACHVLATQSERLLDAPFTVAHLLKVGSYALVLGGALLDNARLFEQVRRLAVTDSLTGL
                     ANYGTLINVMDAEIQRSRRTGRPFAVLLFDLDDLKTINDAHGHLVGSRAICRLANVLQ
                     LHSRTMDTAARYGGDEFAVVLPESDEEAAATVGRRICERLSEDGEFPKATVSVGSAVF
                     PEDGETIDGLFNVADKALYGMKRRPDKFRTLARIAACL"
     gene            complement(5790..6932)
                     /gene="moeB"
                     /locus_tag="VG272_11910"
     CDS             complement(5790..6932)
                     /gene="moeB"
                     /locus_tag="VG272_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_257790190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HWF14436.1"
                     /translation="MKLSREEIQRYSRHLIMPEVGMEGQLKLKKARVLTIGTGGLGAP
                     LGLYLAAAGVGHLGLVDFDVVDSSNLQRQVTFTTADVGKPKSEAAKARLSALNPSIDI
                     VSYETRLTSDNALDLFRDYDIIVDGTDNFPTRFLVNDACVLLGKPNVYGSIFRFEGQA
                     TVFGYPGGPCYRCLYPEPPPPGLVPSCAEGGVLGVLPGIVGSIQAMETIKLILGAGDS
                     LVGRLLLFDALAMRFRELKLKRNPECPMCGDHRTITKLIDYEEFCGIRGEETPAMTEG
                     IQEITATELKARQDRGDKPFLLDVREPHEYQICNLNGKLIPLGELTRRVNELDSSVEM
                     VVHCRSGKRSADAIHFLKTAGFKKLLNLKGGILAWADEVDPRMPKY"
     gene            complement(7022..7315)
                     /locus_tag="VG272_11915"
     CDS             complement(7022..7315)
                     /locus_tag="VG272_11915"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWF14437.1"
                     /translation="MAVKVIIPTPLRQFTGKQSSFECNAATVGEALSGLTGTYSELRK
                     HLFTDEGKIRSFVNVYVNDEDIRFLEKENTKTKDGDTVSIVPSIAGGTAQRNQ"
     gene            complement(7491..7940)
                     /locus_tag="VG272_11920"
     CDS             complement(7491..7940)
                     /locus_tag="VG272_11920"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HWF14438.1"
                     /translation="MLLLTKNLEQDIRDHGAKDYPHECCGAMLGTDNDAAGREVRALF
                     PLINRRDDSPRNRFSITPEDFRAAERAATERGLDLLGWYHSHPDHPARPSEFDREHAW
                     PWYSYVIVSVDNGVPKDLTSWLLEDDRSKFQPEVVAPERVAGESKQS"
     gene            complement(8027..8992)
                     /locus_tag="VG272_11925"
     CDS             complement(8027..8992)
                     /locus_tag="VG272_11925"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HWF14439.1"
                     /translation="MTTFNKTVSAEASANEKLIGRNVLDCIGNTPLLRFQRLGPNFPN
                     AEFYGKAEWFNPGGSVKDRPALEMIRDAERTGKLKPGQMILDATSGNTGIAYAMIASA
                     LGYRVTLCLPTSASEERKKILKAFGADLVFTPGDEGSDGAILRAREIVAAEPDAYFYP
                     DQYGNDSNWRAHYKTTANEIWEQTGGRITHFVAGLGTSGTFMGTTRRLKELNPGIRCI
                     SLQPDASFHGLEGWKHMATAIVPPIYDATLADANLEVGTEEAYRLVKRAAREEGLLLS
                     PSAAAALEGCFQVASGLGADEHAVIVTILPDSGSKYLSERFWDEV"
     gene            9354..9785
                     /locus_tag="VG272_11930"
     CDS             9354..9785
                     /locus_tag="VG272_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14440.1"
                     /translation="MKDRFPAKLMDALSSAGFVVSSTGGVMRVEKYGSGAEFRRTSDN
                     HYTMTILPTILINGQFVRLWDAGYQKFLLTDDGRKFPAHAEQLVNMRKFNEELRTALG
                     VPTYYNEALGSTSLVSVYDRVKGRTGDVPDKTVGVKPVTGH"
     gene            complement(9824..10264)
                     /locus_tag="VG272_11935"
     CDS             complement(9824..10264)
                     /locus_tag="VG272_11935"
                     /inference="COORDINATES: protein motif:HMM:NF017574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HWF14441.1"
                     /translation="MDRKILAAFDQHGSHVLALADFADHSGLKDRLLQLTERGWLRRG
                     EATDTFLRTEDGRLQLAAPCDVTIYSRPGCHLCEEAKAQLAPLLKAFSARLTEINIDE
                     DPELRARYDYDVPVIFLGARKVAKHRVELAQFRRQLRDASTTPE"
     gene            10558..10920
                     /locus_tag="VG272_11940"
     CDS             10558..10920
                     /locus_tag="VG272_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14442.1"
                     /translation="MADAIALIDDLFFLAKVHQTAKLTGVTLETASTGEQLLKAAEGS
                     PSALIMVDLNARQGALDAVEKLCAPSAAGNPRHVIAFLSHVQTDLAERARTAGCSEIM
                     PRSKFTQNLAEILRGAKS"
     gene            11058..12026
                     /locus_tag="VG272_11945"
     CDS             11058..12026
                     /locus_tag="VG272_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14443.1"
                     /translation="MAEATTHKDSVVVTQTLKPALLALAVTFLFTVCVPVSRAQQQQQ
                     IATPPENALSDALSAACRQDSPTFGNSLTAENAAAFRTLPSPQRTAMMKRFVLLDEPG
                     RPLLSTSNSGQKIVRCESPSFTTEMRLGVAKVRENLAFIPMEIPITGEEPRSVTFGMV
                     REGGNWKLLSVGLVLLDVPAMAKQWAQADLDANEDIAIADLRSVATAANAYRNAYGVL
                     PETLEALGPAPSGGVSPDAAGLLDARLAAGEKDGYTFRYAIVPATDFAPGEDSGKAVN
                     FSIACSPKEYGKMGRRSFFLDSSGTLRGADKKGGIADRTDPRIVPS"
     gene            12192..12740
                     /locus_tag="VG272_11950"
     CDS             12192..12740
                     /locus_tag="VG272_11950"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HWF14444.1"
                     /translation="MSDQSDKPISLPEPVIEGLPTADIPERRRVTRYSFIAVAEVFDI
                     RSQTRLEGRCSDLSMGGCYVDTLAPFPIDSALRVTIQHDSREFQAMAVVTYCHPSMGM
                     GIKFTEIEPEFRNVLCYWVVDLCGEPIPKPEPTEAPSSVGQSIDADSNMRLVLNELVT
                     LLVRKKIITEMEGAELLFRALR"
     gene            13022..14161
                     /locus_tag="VG272_11955"
     CDS             13022..14161
                     /locus_tag="VG272_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14445.1"
                     /translation="MASYAMPFCRRHFLRIVCIAAAMLVAATGWASSFSTAPVYFSDQ
                     VATQTTAPVGPSLPIFEMHSGFWSNLHHFLYLQSRLLKTGPPSTQSGRGEAQPDELPV
                     SLIGFPDADIHAWQDAVAFYSKDLGNRDLLLNGDMEIINNQLSLMESCPDLEGKTTPY
                     CKSGLRPDLVEALERAAPVFRAHWWEQQDHANREWISQVAPLIQKMGVELSGQLADVY
                     QRPWPSQRIRVDVVWYGGPFGAYTSLDPMHITISSHDPRNRGVYGFEVLFHEASHAVS
                     GSVTEAIAHDFRARDKPIPRDFWHALLFYTTGEIVRRDIEYGSMTFTSEQKNGASEYL
                     PYAARYGLYSGSWEHFRTLLDLYWLPYLDGRVSFETAMTRMAAEM"
     gene            14566..15429
                     /locus_tag="VG272_11960"
     CDS             14566..15429
                     /locus_tag="VG272_11960"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HWF14446.1"
                     /translation="MPTKPAPGKDLEIKLAHSPDSDDAFMFYGMATQKVRTPGLKFTH
                     VLADIETLNQAAQQEIYDVTAFSFGAYPYLRDKYVLLDCGASFGEGYGPIVVSQHPMK
                     RQDLTKCRIAVPGLQTSAYLALRLYQPNIEAVVVPFDQILDAVKNSVVDAGLLIHEGQ
                     LYFPQMGLHKVVDLGQWWLEQTGLPLPLGGNSVRRALGRTVGLQIARAIHESVSYGLE
                     HREAAVNYAMQFARDMETEMADKFIGMYVNNWTLGYGDRGRQAVNEFLRRGVSEGLIP
                     GSPDAEFLRED"
     gene            15577..16428
                     /locus_tag="VG272_11965"
     CDS             15577..16428
                     /locus_tag="VG272_11965"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HWF14447.1"
                     /translation="MQRILSTYLFVNRKLTADLVGELARAGASAIELFCSRGHFDYRS
                     SDDAREMASWLSGNNLALHSIHSPTTRDFHLNRESGAPLSIFDPERLRRQEAVDEIKR
                     ALDLVEQIPFRYCIQHVARQRDLPDDRKWDAAFSSLEHLSLFARQRGVTLAIENTPGE
                     MATLVNLKNFLEQTRLSNVKLCFDAGHAHLEGGAVEALEAVRDLVVTTHMHDNRGERD
                     DHLLPYEGTIDWDAMLTALPDEAPLVLELKEPPAAVGSTEVQAFAETLRGVQTVFDKF
                     EEERAKN"
     gene            16735..18036
                     /gene="asnS"
                     /locus_tag="VG272_11970"
     CDS             16735..18036
                     /gene="asnS"
                     /locus_tag="VG272_11970"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003037.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HWF14448.1"
                     /translation="MPIVSIQDVGKHDGQEVTLRGWLYNLREAGKLLFPIFRDGTGTI
                     QGVVSLKDQPVAFEALRGLTQESSVIVTGRIHADKRAPGGFEIQMTALEVVQRVPADT
                     PYPIQLKEHGVDFLLDHRHLWIRTPRQASILRIRAEAVRAAHEYMASQDYILTEAPIF
                     TPAACEGTTTLFEVQYIDDEKAYLTQSGQLYVEATAMALGKVYTFGPTFRAEKSKTRR
                     HLTEFWMIEPEAAYAHLEDMMALGEGLVCHVVQSVLKNRARELETIGRDASKLANVTA
                     PFPRITYDEAIQILQQHGNPAKWGDDFGGDEETILSSAYDRPVMVHRYPAAMKAFYMQ
                     PDPDRPDLALGFDMLAPEGYGEIIGGGERVADYDLLLKRLREHNLPEEAFQWYLDLRR
                     YGSVPHAGFGLGLERTVAWICGTEHIREVIPFPRMIYRVYP"
     gene            18086..18997
                     /gene="rocF"
                     /locus_tag="VG272_11975"
     CDS             18086..18997
                     /gene="rocF"
                     /locus_tag="VG272_11975"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HWF14449.1"
                     /translation="MAQKIRIIGVPMDLGQSRRGVDMGPSALRVAGLQSRLKSLGHTV
                     EDIGNVYVRQPEEQPYGEKRAKYLNEIAETCRGLAEMVERSLAEGYFPLVLGGDHSLA
                     VGSFSGVSNYYRAQSKRVGYLWLDAHGDMNTPESSPSGNIHGMPLAAIMGYGAPELVE
                     LLGYKPKVEPRNVVLVGVRDLDPKERRLIKESGVHVFTMRDIDERGMRDVMNEALRFV
                     TDDTDGVAVSLDLDFVDPDDAPGVGTPVRGGVTYREAHLALEIIADSEAMVSLEVVEI
                     NPVIDLHNQTALLGVEMVLSGLGKKIL"
     gene            19200..20372
                     /locus_tag="VG272_11980"
     CDS             19200..20372
                     /locus_tag="VG272_11980"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HWF14450.1"
                     /translation="MSTEKKRLRIGILFGGRSGEHEVSLISAASVISALDPEKYEAVP
                     IGISKEGRWLAGTAAHKMLPPFLESAHGKPALGEVLRTGESVMLSADPNVAALVPANN
                     SRTDALHVDVVFPVLHGTFGEDGAVQGLLDLAGLPYVGSGVLGSAVGMDKDVQKRLFL
                     QAKLPVGEFMTIPRSQWEKSPAKVLREIRKKFRFPVFVKPATLGSSVGMTKAHDAKEL
                     AKAMDFAGEFAQKIMVEKEIRGREIELSVLGNEDPKASIPGEIIPHREFYDYAAKYLE
                     EGTRLMIPAKLTKAQVKKFQEYAVRAFRTLECLGMSRVDFFLENRTGKILLNEINTIP
                     GFTSISMYPKLWEASGLPYRQLLDRLIELALSQHRERQRTKYTIELPATTGSDALK"
     gene            complement(20421..20729)
                     /locus_tag="VG272_11985"
     CDS             complement(20421..20729)
                     /locus_tag="VG272_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14451.1"
                     /translation="MSWYNYVACFFAGATLANFVPHFVHGISGDRFPTPFAHPPGKGL
                     SPPVVNVVWAFVNLLLGCLLYRIGHVSHDDFLTLGLLFAGGVAISTILSFRFAQKLRE
                     "
     gene            20855..21439
                     /locus_tag="VG272_11990"
     CDS             20855..21439
                     /locus_tag="VG272_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWF14452.1"
                     /translation="MRSFVAGRRRLIGTVPRVLGYNAAFRNTLLRILPMLSIRPATVD
                     DAALLAEMIREFAAFEHLEHEVGTTEEDLLRDGFGPRPKFRSVIAEWEGKPVGYAVFF
                     EFYSSFQGRAGLFLDDLYVRPDSRKHGIGTALIKHVAGIAWRGKYFCMRWEVLDWNKP
                     AIDFYKNLGAVFLDEWKPAILIGDALEAAAKGTA"
     gene            21559..22116
                     /locus_tag="VG272_11995"
     CDS             21559..22116
                     /locus_tag="VG272_11995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF14453.1"
                     /translation="MAEQDQKLTETIGREQGRLRNFIRKRVPDEADAEDILQDVFYEL
                     IEAYRLMKPIEQAGAWMFRVARNRIVDRFRKKKPEAFTALKDPDEESGDSLTLEDLLP
                     SPDAGPEAAYARSILMEELDAAIDELPEDQREVFIAHEYDGVSFKEISAETGVSVNTL
                     LSRKHHAVVHLRQRLQTIYDEFLNP"
     gene            22128..22466
                     /locus_tag="VG272_12000"
     CDS             22128..22466
                     /locus_tag="VG272_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14454.1"
                     /translation="MRGNRFLRVLKFIAIGAVAFTAFGFVVTYLWNYLMPGLFGLHAI
                     TFWQAIGLMLLGRLLFGGFRPGRGGFGWRGGEMRRRWEKMTPEEREKFREGMRGRCGS
                     PRTPAPQPNQ"
BASE COUNT         5128 a         6287 c         6038 g         5186 t
ORIGIN      
        1 ccgacgagaa agaactggtc ggacaatttt caaacctggt gatgtttcac catacggcgg
       61 aagagttcac cgtgcatttc atttatgttt ttccaaatgt gccgcaaggc aaattggtct
      121 cgagcgtcat cgtcagcccg ggacacgcca agcgcatact gcgcgcgctc gaggaaaata
      181 tcaggcggta cgaaaaacaa ttcgggccta tccgcgaagt tcccgaccct acccccgtcc
      241 ccaatgtcgg ctttgtacag taggaaattt cgcttccttt ttgcaaattg aaatcacgca
      301 aggccggtgc ggacttcacc ccaatccaat atggatttgt gtttcctcat acaagcgata
      361 acacgttagt tataaggggt ttacatatct agttcactta tcgaaacatt agcggttgaa
      421 gttcgcttgg gtccgaagct acccatgtta aattagagcg ctagagcctg catcaggctg
      481 gctattttgc ttcaaacagg gtagaggcgc cctcgggcga gttacctacc ttgcgtaaga
      541 acggagttcg tacccgcaaa tacttatgaa agccgtttgc tccagttgtg gcgcacagca
      601 ttcccttccg gataagcaac tggttggtct gccccgcgtc caatttcagt gtgccaagtg
      661 cgggaaaaat acggttgttg aaatctccgc cagtccggac gccacccaaa ttttgacgcc
      721 cttgcccgga tttgcccgca gcgccggcgc gccctggctg gccggcagca gcgcgaatga
      781 agcgaattcg ctgcagttgc ccacgggaaa ggctatcgcc ctttccgtga ttgccggacc
      841 ttcccgcggt cttgtctatc ccatcgaaaa accacgcgtg gttttgggca gagccgacgc
      901 ggacgttgtg atcaacgaca aagaaatttc gcgctggcat tgctccattg aaattaaggg
      961 tgatgtgatc cggttgcgcg acatggattc gaccaatggc acttacttcg ccgatgaacg
     1021 tgtccgcgtg gccgaattga agcatctctc cgaattccgc attggcactt ccgttattct
     1081 tctgagcgtt acccccaagc ttgctcccac gcatgcaacg aaaataagtt gatcctctcg
     1141 atcatgcgga attctttaat aaatcagcgt ggattgcgtc cgcccgaccc gccgcatttc
     1201 gtgttaccgt aaccgattgg attcccgatg ccccgtctga ctgccacttt gatttcttac
     1261 aacgaagaag tggacctgcc gcgcgcgttc gcgtcgctca cgggcattgc ggacgaaatc
     1321 attctggtgg attccggctc tacggaccga accaccgaag tggcgcaatc cttcggcgcc
     1381 cgcgtttaca cccgcaaact ggacagcttc gcgacacaaa aaaattatgc cgcttccctt
     1441 tccagcaatg actggatctt ttccatggat tgcgacgaag aattaagtcc tgagctgcgt
     1501 gcctccctgc ttgcgtggaa agaacaaaca cctgaaaaaa atggctatga aattctactg
     1561 ctgacaaatt atctgggcgg atggattcgc cactccggct ggtatccgga acgcaaattg
     1621 cttctttatc gacgtgataa aggacaattt gaaagcgcgc ttcacgaacg cgttcatctc
     1681 gaaggctccc cgggccgcac gcagggccac ttgtttcatt acacgattcg atctctcacc
     1741 gagcatcgag cgaagcttga cgccatgacg acgctggcag ccgaagacat gtttgcccga
     1801 gggtacaaga tttggcgccc cgcgatgatt tttgccgcgc cgtggaccat ccttcaacgg
     1861 ctcgtgtttc agggcggcat tctggacggc cgtcgcggct ggctgatcgc ctggttttcc
     1921 gcgaaatata tttacgttaa atatcgcaaa ctcggccagc ttctcggcgg caaacaactt
     1981 tcgcaccgct cctggcccag tcctggcggc gtgtgagtat gcgcccgcta cttgtcgatc
     2041 tgggccggga ttaccgcgga ggacaagatc aggcgcttct gctcttgaaa ggcctcgcag
     2101 cgctgggcca cacgccggaa tttattacca ttcgcggctc gcttctcgcc gaacgcgcac
     2161 aaagtgcggg catccatgtg catctggtcg acaagcgctg gcggcgcctt tccgcggtgt
     2221 ttgccattcg cgaactgctt tcccggcgcg ttgcggatat cgtccacgca aatgagccgc
     2281 atgcgttgac ctccgcatgg cttgcaagag cgcatcatcg tgtgccactc gttgcatcgc
     2341 gtcgcgttat ttttccactt tccaaagacg cggtttcgct tgcccgttat cgttccgctg
     2401 caaaaataat cgccgtgtcg cagtgcgttt cctccgcgct cataaactca ggtatacagg
     2461 ctggcaatat tacagttatc tctgatggcg tccctctcga aaatgcgttt tcgcaggaag
     2521 atcgcgaaca agcgcgtcga agcttgggaa tcgaaccgga tgctttactc atcggctgca
     2581 tttccgcatt aactcccgat aaaggacatg aaattctgat ccgtgctttt gcacttatcc
     2641 gcgcacgttt tcccggttgc caattgctgc tcgtcgggga tggaccgtgc cgggcgaaac
     2701 tagccgcgcg ggtgcgggaa cttgatctcg atggcgtcgt tcacttcaca ggttttgtag
     2761 aagatataca acgcgtttac gcagccatcg atttgttcgc tttcctggcg caagcggaag
     2821 ccttgggcac cgcactgctc ttagccatgg cacatggctt gcccgttgtg gcaagtgcgc
     2881 gtggaggaat tcccgaagtc gtgcaagatg gaaaaaatgg tttgctgctg aaagattcaa
     2941 gccccgaagc cgtcgcgtcc gcaatcgagc ggctgatttc tgattcagat tcagcagcac
     3001 gcttaggcgc cgccgcaagg gaaacaattc tcacgcactt ctctcaagaa catatggtca
     3061 gacagacgct cagcctgtac gagcaactcg tggcagaccg ttcgagccaa atgtaagtta
     3121 gtacttcatt caatttagaa taatctggtt cgcaatttgc aaatcccaca tcacaaatca
     3181 attcatgatt tacgagcagc gaatcacaag tcactattca ctactcactc gtcacgcctt
     3241 ctcctcccca aacgcaccaa aagcgcctgt catcccgttc ccgcggaatg tcagacgctt
     3301 ctgggtaaat ccttacgcca gttgtttgcc ttcttcgcgt gtccctccta tccttgcagc
     3361 ggtttccctg agccaactgg agcggtaatc acccaccggc cattgggctc taggaactca
     3421 agtccgatga catagccatc gggatgcggc cggcaatacg ccacacgcgc taaggcgcga
     3481 aaatcgccgg gcaacgattc aaaattcaat atttcattcc ggttccatcg acgagcgctc
     3541 ggcactcgag ccccgcgtga actaacgtcc tccgtaaacg tcgtctccac gcaagtggag
     3601 gactcgcgct cggaaatgtg gacagccacc gtcatcggaa ttcttttttc aagtcgcgag
     3661 atatcaaatg ttgctggcat atttccctca gtcttgcggt gccgtttctg cctaattcat
     3721 tcttggctct agaggcaagc cgcaattctt gccaacgtcc gaaacttatc cggtcgccgc
     3781 ttcattccat acagcgcctt gtcagcgaca ttgaacaatc catcaatcgt ttccccgtcc
     3841 tccggaaaca cggcagaccc tacgcttacg gtggctttcg gaaattcacc gtcctcggac
     3901 agtcgctcgc aaatccgccg gcccaccgtg gctgccgctt cctcatccga ttcgggcaac
     3961 acaacagcaa actcatcgcc tccatacctc gctgcggtat ccatcgtccg ggaatggagt
     4021 tgaagcacat tagccagccg gcaaatcgcg cggctaccca ccagatggcc atgggcatcg
     4081 ttgatcgttt tgaggtcatc cagatcgaac aacaagactg caaacggccg tcctgtgcgc
     4141 cgcgaccttt gaatttccgc gtccatgacg tttatcagcg tcccgtagtt cgcaaggcca
     4201 gtcagagaat ccgttaccgc aagccgacgc acttgttcga acagtcgcgc gttgtccagc
     4261 aacgcgccac ccagcacgag cgcgtagctc ccgactttca acaaatgtgc gactgtaaac
     4321 ggtgcgtcca gcaatcgttc agactgcgtg gccagaacat ggcaagccac gttcatccac
     4381 agggctatgc aaaatatgga atcgaacggg gagggattac gtcgcgacct gcgggtgaac
     4441 cctatcgctg cgaccgcaaa caaaacagcc ggcaagatat cgcctgttct cgaaacgatg
     4501 gctcgggggt gcatgacctg ggccaggggg gaccacaggt aagccatgct cgtcgcgtag
     4561 gcgacagcga ctaccacgaa caaggcgacg gcaatttcct tgcttggttc gcgcgagttc
     4621 ggcacccggc gctcaacaaa taatgcagca atcagtacca gagccagtaa agtcctgctc
     4681 accatccagg taagaggcac gtgtaactgg ctataagcgt cggatgcttg cacatcataa
     4741 aaattaaatg tggcggctgt ttcgatcgca cctgagagca cgaatccaaa cgcaagaatg
     4801 agcgtcagac gatcgcgcgt cccgcgaaac ctcaccatcg catttgccgc ataggtgaag
     4861 caaagcaggc tgccgacgat ttgaaagtag gaatagagta tcggctcgga ggaaaaagga
     4921 agttgtctta gccagacaac aaatatcagg aacgctgccg cgcccaatat tccgagaaac
     4981 caccgaaggc gataactcca cacctgaatt tgacttagca cactcaccgc tcccccagtt
     5041 tgagcagttg catgagccca atttcttagt acggaatgag agggagcaag ttcagtgccg
     5101 atttgaccaa gattttattt tctttgtatc tcactcttat tagaagactt gttaaaagta
     5161 gctcttcagc atgtggagga gtggggccaa gcgttccact ggaatggata gaatcgttac
     5221 aatgactccg gagtccttct cattttgaaa cacactagtt gcaagtttga cactaccgcc
     5281 ttgcagattc gtgtggaaat tagaatttta tgagattgct gcgattgaat ttagattcgt
     5341 taatgacttt tcagacagtt cggtttgttt tgggaaacga cccaggaaaa tacttctttg
     5401 tcctattcaa cgcgcatttt tgaggaaagt tttaggaaac acaatcgcta ggaatggcct
     5461 gagaaaactc cggaacaaag tctaaacctt tggcactggc ggaggaagat ccctggcctc
     5521 ttgcttatcc tgaaaacatg ccgacctgtg agatccatcg caaaaaggtt tttttgccga
     5581 atgcccacag cggcacaaaa ctatggaact gcgcccgcct aaaccgtacg gtttgccttc
     5641 catatccact aattcaaact ccccttccac taccagggga ccatcattcc ggactttgat
     5701 ttttgtcggc gacatcgtag ttctttcatg cggacccact tctcaatccc tcgattttct
     5761 gaatcgagaa gcgggcccaa attcttccct tagtatttcg gcattctcgg atcgacttca
     5821 tccgcccacg ccagaatgcc gcccttgagg ttcagcaatt ttttaaaccc agccgtctta
     5881 agaaaatgaa ttgcatcggc cgagcgcttt ccgctacggc agtgcacaac catttccacc
     5941 gagctgtcca attcgttgac gcgtcgcgta agctcgccca gcggaatcag tttgccgttt
     6001 aaattgcaaa tctgatactc gtggggctcg cgcacgtcca gcaaaaatgg cttatcgcca
     6061 cggtcttgcc gcgccttgag ctccgttgct gtgatttcct gaataccttc cgtcattgca
     6121 ggggtctcct ctccgcgaat tccgcagaat tcttcgtaat caatcaattt tgtaattgtc
     6181 cggtgatcgc cgcacatcgg gcattccgga ttcctcttca atttcagttc gcggaaacgc
     6241 atggccagcg catcgaaaag caggagccgt ccaaccagcg aatcgccggc ccccagaatc
     6301 agtttgatcg tctccatcgc ctggatggag cccacaattc cggggagcac accaagcacg
     6361 cccccttcgg cgcacgatgg gaccaggccc ggcggaggcg gctcagggta caagcagcga
     6421 tagcatggcc cgcccggata tccgaaaacg gtggcttgtc cctcaaaccg gaaaatggaa
     6481 ccgtacacat tgggtttgcc gagcagcacg cacgcgtcgt ttaccagaaa gcgcgtgggg
     6541 aaattgtcgg ttccatccac aatgatgtcg taatcgcgga acaggtcgag cgcattgtcg
     6601 ctggtcaagc gagtctcata cgaaacgata tcgatcgatg ggttaagcgc ggaaagccgg
     6661 gctttcgccg cctcggactt cggtttgcct acatcggctg tagtgaaagt cacctgtctc
     6721 tgcaaattcg acgaatcgac gacgtcaaaa tccacaagcc ccaggtgtcc gacaccggca
     6781 gctgcgagat acaggccgag tggcgcgccc aatccacctg tgccaatcgt cagtactcgc
     6841 gctttcttga gcttaagttg tccctccatg cccacttccg gcatgatcag gtggcggcta
     6901 tagcgctgaa tttcttcccg ggaaagcttc acatcctgct tgtttaagtt gtcagtctgg
     6961 attgccacgg acgttctcct ctaatttctt ctaatctaat gtgttcgcga atgtcgcgga
     7021 cttactgatt cctttgcgcg gtaccgccgg cgatcgatgg cacgatgctg accgtgtcgc
     7081 catccttggt tttcgtgttt tccttttcca gaaaacggat gtcttcgtca ttcacgtata
     7141 cgttcacgaa gctgcgaatt tttccttcat ccgtaaacaa atgttttctc aactcggaat
     7201 acgtaccggt caggccgcta agcgcctcac ccacggtggc cgcgttacat tcgaagctgg
     7261 actgcttccc ggtaaattgg cgaagcgggg ttggaattat aacctttacg gccatacgga
     7321 cgtttctcct tgttgaagtc ggagggtgtc tgtcaccctt gcgctaaata aattcctcaa
     7381 aaccgctcgc cgcatccagg tactttgaac aaatcacaat atgagcgaat caccacatcg
     7441 gatgcgcgag gacctatcta aggttcaagc ttaaacatcg ggcggaaagg tcaactctgt
     7501 ttgctctccc ccgcaacccg ttccggagca accacttccg gctgaaactt cgaacgatcg
     7561 tcttccaaca gccaggaggt caaatccttc ggtacgccgt tgtcaacgct gacgataacg
     7621 tagctgtacc acggccatgc atgttccctg tcaaactcac tgggccgggc cggatgatcc
     7681 gggtgggaat gataccaccc caggaggtcg agtccacgtt cggttgcggc acgttcggcg
     7741 gcacgaaaat cctcaggcgt aatcgagaag cgattgcgag gagaatcgtc tcgccggtta
     7801 atcagcggaa acagcgcgcg gacttcccgt cctgcagcgt cgttatccgt accgagcatg
     7861 gcgccgcagc attcgtgcgg ataatccttt gcaccgtggt cgcgaatatc ctgttccaaa
     7921 ttttttgtta gcagcagcat cgcgcaagtc tccaggagtt tgttgcccgc atccgtaatt
     7981 cgccttttgt agcccgcata tcgccggtgc gcagcaactt actttcttac acttcgtccc
     8041 agaaacgttc actcagatac ttggagcctg aatccgggag aattgtaacg atcaccgcgt
     8101 gctcatccgc gccgagtccg gaggcgacct gaaaacatcc ttcgagcgcg gccgcagcac
     8161 tgggactgag caacaaaccc tcttcacgcg cggcccgctt gacgaggcgg tacgcctctt
     8221 ccgttcccac ctccaaattc gcgtcggcaa gcgtcgcgtc atagatcggt gggacaatcg
     8281 ccgtggccat gtgcttccat ccttcgagtc cgtggaagga agcgtccggc tgcaggctga
     8341 tgcagcggat cccgggattt aattccttaa ggcggcgcgt tgtgcccatg aatgttccgc
     8401 tcgtgccgag ccctgcaacg aagtgcgtaa ttcgtccgcc cgtctgctcc cagatttcgt
     8461 tggccgtggt tttgtaatga gcgcgccagt tggaatcatt gccgtattga tccggataaa
     8521 aatatgcatc cggctcggca gcgacgattt cgcgcgcgcg caatatcgcg ccatcggagc
     8581 cttcgtctcc cggagtgaag actaaatccg cgccaaatgc tttaagaatt tttttgcgtt
     8641 cctcgctggc gctggtggga aggcaaagcg tcacgcgata gcctagagca gaggcaatca
     8701 tggcataggc gattcccgta tttccgctcg tcgcgtccag aatcatctgg ccaggcttca
     8761 attttccggt gcgttccgcg tcacgaatca tttccagcgc cggacgatcc ttcaccgagc
     8821 cgccagggtt gaaccattcc gccttcccgt aaaactccgc attcggaaag ttgggcccga
     8881 gccgctggaa gcggagaagt ggcgtattcc cgatgcaatc gagcacgttg cggccgataa
     8941 gtttctcatt cgcggatgcc tcggccgaaa cggttttatt gaatgtggtc atagatcaca
     9001 atattttcta gttgcagccg cgagactcgc gacgggcttt gagcgaacgc aaaagccgca
     9061 cccgtcgatc gaaacctggt ggcttcttcg tatttaccta atttagcata atctccgcca
     9121 atctgtggtg gtcagggcgc aatccactca ggcgtctccg cttctctcct cttttctctg
     9181 tgctctctgt gttaaagcct tttcgggcgg aaagggcaag gccttaacac ggagaacaca
     9241 gagaaaagaa gagggccacg gagaattcgg cgaaggttca ttgtgagttc ccagaatgaa
     9301 tttggccttt acagcaaatc tcaccgcccg tattcttgaa ggctatgtgg aatgtgaaag
     9361 atcgatttcc agcgaagttg atggatgcat tgtctagcgc gggtttcgtg gtgagcagca
     9421 cgggcggcgt gatgcgcgtt gaaaaatacg gcagcggcgc ggaattccgg cgcacttcgg
     9481 acaaccacta cacgatgacg attttaccca ccatcctgat caacgggcaa tttgtgcgtc
     9541 tctgggatgc cggctatcag aaattcttgc ttacggatga tggcagaaaa tttccggcac
     9601 acgccgagca actcgtcaac atgcgcaaat ttaatgagga actgagaacc gctctcggcg
     9661 ttcccacgta ttacaacgaa gcgctcggct cgacctccct ggtgtccgtc tatgaccgcg
     9721 tgaaaggccg caccggcgat gtgccggaca aaacggttgg cgtgaagccc gtaactggtc
     9781 actaaaaatc agatttcgca acgattaaaa ttcaatagag aaattattct ggagttgtgg
     9841 aggcgtcgcg aagctgtcgc cgaaattgcg cgagttcgac acgatgcttg gcgactttcc
     9901 tggcgccgag aaaaatgact gggacatcgt aatcgtagcg ggcgcgaagt tccggatctt
     9961 cgtcgatatt gatttccgtg agacgcgcgc tgaacgcttt caaaagagga gcgagttgcg
    10021 ccttggcttc ttcgcacagg tggcaacccg gacgcgaata aatggtcaca tcacaaggcg
    10081 cggccagttg caaacgtcca tcctcggttc gcaggaaagt gtccgtggct tctccgcggc
    10141 gcaaccagcc tcgctcggtg agttgaagca ggcgatcttt caagccggaa tggtcagcaa
    10201 aatcggctag ggccagtaca tgcgagccat gctgatcgaa agcggccaga atttttcggt
    10261 ccattgcatc catcacgctg atatttgtat ctcaatttaa tctgtacggg aagcacgcag
    10321 tccctcaatt tgagaaacaa cctgccctct ccgtcccaaa attcgtggga cggagaggcc
    10381 gctacaaaac cggaacgcat tgcgatgttg gttttgtaga ggccgcccgt attgtgggcg
    10441 ggcagctttt ccggttgtgc aattcaaatt gatgcactga ccccacgcgt tgcggcttgt
    10501 gttgcggata cgtacgtggt aaatcggaaa ggcgaactat cgtgaggatt gaaattcatg
    10561 gctgacgcaa tcgcattgat cgatgattta ttttttctgg ccaaggtgca ccaaacggcc
    10621 aaattgacag gcgtaacgct cgagacagcg tccaccgggg agcagttgct gaaagcggcg
    10681 gaaggcagcc cctcggcgct gatcatggtg gatttgaacg cgaggcaggg agccctcgac
    10741 gcggtagaaa aattgtgcgc gccaagcgct gcaggcaacc cgcggcacgt gattgccttt
    10801 ctatcccatg tgcaaaccga tttggcggaa cgcgcgcgta ccgcaggatg ctcagaaatt
    10861 atgcctcgat ccaaattcac gcaaaatctc gccgaaattt tgcgcggagc aaaatcgtga
    10921 gtgggacagg ctccaacccg tccgcctttt ttatgcgtcg ggcaaaaact aaaaccggga
    10981 ggttgagaag tctgtgtgag aactgcaaag ttgaacttca gcggctaaag ccgcactcat
    11041 tttgcagggc ttatgtcgtg gctgaagcca cgacccacaa agattctgta gttgtcacac
    11101 agactctgaa gcccgcccta ctggcgcttg cagtaacatt tcttttcact gtttgcgtcc
    11161 cagtctctcg cgcgcaacag cagcaacaga tcgcaacgcc acctgaaaac gcgctttccg
    11221 atgcgttgtc cgcggcttgc cgccaggatt ctccgacatt cgggaattct ttaaccgccg
    11281 aaaatgccgc agccttccga actcttccca gccctcaacg cacggcaatg atgaagcggt
    11341 tcgtgctgct cgatgagccg ggcaggccat tgctctcgac cagcaatagc ggtcagaaaa
    11401 ttgttcgctg cgaaagccca agtttcacaa ccgaaatgcg gcttggggta gcaaaggtac
    11461 gcgaaaacct cgccttcatc cccatggaaa tccccatcac gggagaagaa ccacgtagcg
    11521 tgacgttcgg catggtgcgt gaaggcggaa actggaagct cctctccgta ggactcgttc
    11581 tgctcgacgt gcctgccatg gccaagcagt gggcgcaggc agacctcgac gcgaatgagg
    11641 acatcgccat cgccgatttg cgctcagttg ccaccgcggc aaatgcttat cgcaatgcgt
    11701 atggagtatt gcccgaaacg ctcgaggcgc taggtccggc accctccggt ggagtttcac
    11761 ccgacgctgc cggactgctc gacgctcgtc tcgccgcggg ggaaaaagac ggctacacat
    11821 tccgttatgc aattgtgccc gcaaccgact ttgcgccggg ggaagattcc ggcaaggcgg
    11881 taaacttctc cattgcctgt tcccccaagg aatacggcaa gatgggacgg cgctcgttct
    11941 tcctggactc gtccggaacc ctgcgcggcg cggacaagaa gggcggcatc gcggaccgca
    12001 cagacccgcg catagttccg tcttaacccc gtcgcgtttg catcacattc agccaacaat
    12061 cttcttgcta gcggtacgcg gctgattcac aattatttca tccttccgat atccgtgctg
    12121 gaatccgcgc ggaatccctt acctaaatga atgcctgcac ggatgtgtgt tctgcgactt
    12181 tgagcgcaaa aatgtccgat caatccgata aaccaatttc gcttcctgaa cccgtgattg
    12241 agggattgcc gaccgccgat attcccgaac gtcgaagagt gacgcgatat tcttttattg
    12301 cagtggcgga ggtttttgat attcgctcgc aaactcgttt ggaagggcgc tgctccgatc
    12361 tgagcatggg cggatgttac gtcgatacac tggctccctt cccaatcgac tccgcgttac
    12421 gcgtcacaat ccagcatgat tcgcgtgaat ttcaggccat ggccgtcgtg acctattgcc
    12481 atccgtccat gggaatggga atcaaattca cagaaattga gcccgaattc agaaatgtgt
    12541 tgtgttattg ggtcgtggac ctctgcggcg agccaattcc caagcctgag ccaacggaag
    12601 cgccttccag tgtggggcaa tccattgacg cggattccaa catgcgtctg gttctgaatg
    12661 agctcgtcac cttgctggtg cgaaaaaaaa ttattacgga aatggaaggc gcggaattat
    12721 tatttcgagc gctccggtga acttttaaga ctgcgctctt ctcaaatcaa ttcaatcttt
    12781 tcaattcctc tgtagctcgt ctctcttgtc ctgagtgaaa acgaaggatc agaggcgaga
    12841 atttagatga aagtgcgaag ccatactgac agtttcagcg caacgtgccg gttagcaagt
    12901 gccgctcccc gaacttcgat agccttattt attttagcgt ttgagcagac atcgcctaaa
    12961 cccccgcctc tgaagaggcg ggctacagca attctggcaa ggtgcccagt ttggcgataa
    13021 tatggcaagt tacgccatgc ccttttgccg ccgtcatttt ttgcgaattg tttgcatcgc
    13081 agctgccatg cttgtagctg caacaggctg ggcatcctcc ttttcaacag cgccagttta
    13141 tttttctgac caagtcgcca cgcagacaac tgctcccgtg ggcccgtcgc ttcccatttt
    13201 tgaaatgcac agcggctttt ggtccaactt gcatcacttt ctctacttgc aatcgcgcct
    13261 cctgaaaacc ggtccaccct cgacgcaatc agggcgaggc gaggcgcaac ccgatgaact
    13321 acccgtttcg ctcataggtt ttccggatgc ggatattcat gcctggcagg atgccgtcgc
    13381 gttttactcc aaggatttag gaaatcgtga cctcttattg aatggcgaca tggaaattat
    13441 taacaatcag ctttccttga tggaatcgtg ccccgatttg gaaggaaaaa ccacgcccta
    13501 ctgcaagtcc ggtttgcgtc ctgacctggt cgaagccttg gagcgcgccg ctcctgtatt
    13561 ccgcgctcac tggtgggagc agcaggatca tgccaaccgg gaatggattt cccaggttgc
    13621 gccgctgatt caaaagatgg gcgtcgaact ttccggccaa ctggcggatg tataccaacg
    13681 gccttggccg tctcagcgaa tccgtgtgga cgtggtttgg tatggggggc cattcggggc
    13741 gtacacctcg ctcgatccca tgcacataac gatttccagc catgacccgc gcaatcgcgg
    13801 cgtatacgga tttgaagtgt tgttccatga agcttcccac gcggtctccg gttctgtgac
    13861 cgaggcgatt gcccacgatt tccgggcgcg cgataaaccc atcccgcgag atttttggca
    13921 cgcactcctc ttttacacaa caggcgagat cgttcgccgc gacatcgaat atggctcgat
    13981 gacctttacc agcgagcaaa aaaatggggc gtccgagtat ctcccgtatg ccgcgcgtta
    14041 cggtctctac tcgggctctt gggagcactt tcgcaccttg ctggacctgt attggctgcc
    14101 gtatctggac ggccgagtct cgttcgaaac cgcaatgacc cgcatggccg cggaaatgta
    14161 actcacttca gggatgccgc cgcagtttgc gaaataacgt gccctctctg tcccaaaaaa
    14221 cgtgggacag agagtcctct acgaaaccaa attgccttgc tttcatagcg cccggccgtt
    14281 tttcgggcgg gcagctcttg tgtattggca atctaaatgg atgctctgcc aacagtatcg
    14341 cgacagtgct ccaattgtcc gccaacgccg agtgtgctac tcttttgcac tcaaagcgcg
    14401 gctttctgta attggattcc gcctcagaag ttcctctctg taacgcgggt gtggcgccgg
    14461 tcatgtgcga ctccagagtg cctggcaata aatcaggccc gagtgaattt gtggccgcct
    14521 ctcccaattc agagaaatac cgaaagggag tgaacgcaga acgcgatgcc gacgaaacca
    14581 gcacccggca aagatttaga gattaaattg gcgcattcgc cggattccga cgacgccttc
    14641 atgttctacg gcatggcgac gcagaaggtg cggacgcccg ggcttaagtt cacccatgtg
    14701 ctcgcggaca ttgaaacgct gaatcaagcc gcacagcagg aaatctatga tgtgacggca
    14761 tttagctttg gcgcttatcc gtatttgcgg gataagtatg tgctcctcga ttgcggcgcg
    14821 agcttcggcg aaggctacgg gcccatcgtt gtttcgcagc atcccatgaa gcggcaagac
    14881 ctgaccaagt gccgcatcgc tgtccccggc ttgcagactt ccgcgtatct ggcattgcgc
    14941 ttgtaccaac cgaacatcga ggcggtggtt gttcccttcg atcagatttt ggacgcagtg
    15001 aaaaattccg tggtcgatgc cggcttgctg attcacgagg ggcagctcta tttccctcag
    15061 atgggactgc acaaggtcgt cgatctcgga caatggtggc tggaacaaac cgggcttcca
    15121 cttccgctcg gcgggaactc tgtccgccgc gctttgggcc gcacggtagg tctgcaaatc
    15181 gcgcgcgcca ttcatgaaag cgtttcgtat ggcctcgaac atcgcgaagc cgcggtgaat
    15241 tatgcgatgc aattcgcgcg agacatggaa accgaaatgg ccgacaaatt tatcggcatg
    15301 tatgtaaaca actggacgct cggatacggc gatcgcggca ggcaggcagt caacgaattc
    15361 ctgaggcgcg gagtcagcga ggggttgatc ccgggttctc ccgatgccga gtttttgcgc
    15421 gaagattaat ttccagaaaa taacgcggga ttaaatatgt gtttgatttc gcattccgcc
    15481 gcacggccgg agtggcgcgc cttgacgcct ataagggcat cgcttagagt cgaagcgaat
    15541 tgatttgaaa tcaaataggg cccgcacatc gtgaagatgc aacgcattct ttccacatat
    15601 ctttttgtaa atcgcaaatt gaccgccgac ctggtgggcg aactggcgcg cgcgggggct
    15661 tccgcaattg aattattttg ctcgcgcgga cattttgact atcgctcgag tgatgacgca
    15721 cgcgagatgg cttcatggct ttcgggaaat aatctcgcgc tgcattccat ccattcgccg
    15781 acgacgcgtg attttcatct caaccgcgaa agcggcgcgc cactctcgat cttcgatccg
    15841 gaacgtttgc ggcgccagga ggcggtggac gaaatcaagc gcgcccttga cctcgtggag
    15901 cagattccct ttcgctattg cattcaacat gtggcgcggc agcgcgatct tccggatgac
    15961 cggaaatggg acgcggcgtt cagttcgctc gagcatctgt ctttgtttgc gcggcagcgc
    16021 ggcgttactc tggccattga aaacacgcca ggcgaaatgg ctactctcgt aaacctgaaa
    16081 aattttctcg agcaaactcg cttgtcgaat gtgaagcttt gctttgacgc cggtcacgcg
    16141 catctggaag gcggcgcagt ggaagctctg gaagccgtgc gcgatcttgt ggtgaccacg
    16201 cacatgcatg acaaccgcgg cgagcgcgac gatcatctgc tgccctatga aggaacaatc
    16261 gattgggacg ccatgctcac agcgctgccg gacgaagcgc cgctcgtcct ggaattgaaa
    16321 gaaccgcccg ccgcggtagg ctccacggaa gtacaagcct tcgctgaaac attgcgcggc
    16381 gtgcaaaccg tattcgacaa attcgaagaa gagcgcgcga agaattaatg cccagccgca
    16441 acgggttgct gtagcccgcc tctcttgtcc tgagtgaaaa cgaaggatca gaggcggggg
    16501 tttagatgag gccggctcga agctcgaaac ataggcgccg atgtggggca gcggcattta
    16561 gcatttagca tttagcattt agcatttagc atttagcatt tagcacgcgc actgcccaat
    16621 ttaattttgg cctcgcgctt tcatctaaac cctcgcctct gaagaggcga gctacagtgc
    16681 gacgcaggcg agcggcattg caaacgtaac aaaaaagggc aaatcacgaa acgtatgcca
    16741 atcgtatcga ttcaagacgt gggcaaacat gacgggcagg aagtcacgct gcgcggctgg
    16801 ctttataatt tgcgcgaagc cgggaaactt ctcttcccta tttttcgcga cggcacggga
    16861 acgatacagg gcgtggtctc gctgaaggat cagcccgtgg cgtttgaagc gttgcgcgga
    16921 ttgactcagg aatccagcgt cattgtgaca ggacgcattc acgcggacaa gcgcgcaccc
    16981 ggcggatttg aaattcagat gacagcgctg gaagtggtgc agcgcgtccc cgccgatacg
    17041 ccttacccga tccagctcaa ggaacacggc gtcgattttc ttttggatca ccgccacctc
    17101 tggattcgta cgccgcgcca ggcttccatt ctgcggattc gcgcggaagc cgtgcgcgcc
    17161 gcgcatgagt acatggcatc tcaggattac atcctgaccg aagcgcccat tttcacgccg
    17221 gcagcctgcg aaggcacaac cacgcttttc gaagttcaat atattgacga cgaaaaggcc
    17281 tacctcacgc agtccggcca gctttatgtg gaagccacgg ccatggcgct gggcaaggtc
    17341 tacacattcg gtccaacgtt tcgcgccgaa aaatccaaga cgcgccgcca cctcaccgag
    17401 ttctggatga tcgagccgga agctgcctac gcgcacctcg aagacatgat ggcgctcggc
    17461 gagggtctcg tctgccacgt ggtgcaatcg gtgctgaaga accgcgcgcg ggaacttgaa
    17521 accattggcc gcgacgcgtc caagctcgcc aacgtcaccg ctccttttcc ccgcatcacg
    17581 tatgacgaag cgattcaaat tcttcagcag cacggcaacc ctgcgaaatg gggcgacgat
    17641 tttggcgggg atgaagagac aattctttca agcgcctatg accgccctgt gatggtgcat
    17701 cgctatcccg ccgcgatgaa agcgttctac atgcagccgg atcctgaccg tcctgatctg
    17761 gcgcttggtt tcgacatgct cgctccggaa ggctacggcg aaatcattgg cggcggcgag
    17821 cgcgtagcgg attacgattt gctcttgaaa cgcctgcggg agcacaacct gccggaagag
    17881 gcgtttcagt ggtatctgga tttgcgccgg tacggaagcg tcccgcacgc gggctttggc
    17941 ctgggtctgg aacgcacggt ggcatggatt tgcggaactg agcacattcg cgaagtcata
    18001 cccttcccac gcatgatcta cagagtctat ccttagtctt gccactgtcc tgactgttcg
    18061 ggactggccg tgctgattaa aaaatatggc acaaaaaatc aggattattg gcgttccgat
    18121 ggacctcggc caaagccgcc gcggagtgga catgggtccc tcggcgctgc gcgtggctgg
    18181 actgcaatcg cgcctgaagt cgcttggcca cacggtcgaa gatattggca atgtttatgt
    18241 aaggcaaccg gaagagcagc cgtacggcga aaagcgcgcc aaatacctga atgaaattgc
    18301 ggagacttgc cgcgggcttg cggagatggt cgagcgctcg cttgccgaag gctatttccc
    18361 gctggtgctg ggcggagatc actcgctggc ggtgggttca ttttccgggg tatcgaatta
    18421 ttatcgcgcg caatccaaac gtgtcggcta tttgtggctt gatgcgcacg gcgacatgaa
    18481 cacgccggaa agttctccct ctggcaatat tcacgggatg ccgctcgcgg ccatcatggg
    18541 ctatggagcg ccggaactcg tggagttgct cggttacaaa ccgaaagtgg aaccgcgcaa
    18601 cgttgttctc gtgggcgtgc gcgatctcga tccgaaagag cgccgcctga tcaaggaatc
    18661 cggcgtgcac gttttcacca tgcgcgatat cgatgaacgc ggcatgcgcg atgtgatgaa
    18721 cgaagcgcta cgctttgtga ccgatgacac ggatggcgtc gccgtcagcc tcgacctgga
    18781 ttttgtcgac ccggatgacg cgcccggcgt cggtacgccc gtgcgcggcg gcgtaacgta
    18841 tcgcgaagca catttggcgc tggaaattat cgcggacagc gaggcgatgg tatcgctcga
    18901 agtcgtcgaa attaacccgg taatcgacct gcacaatcag acggcgctat tgggcgtgga
    18961 gatggtcctt tccggactag gaaagaaaat tttgtaacag ttttcgcccg ccctgtagcc
    19021 cgcctcttca gaggcggggt ttaggcgatg tccttctcaa ggatttaaat ttaggcttcg
    19081 atgtacgggc agccgtattt aacagcaaac gcatgcactg accattttaa ttttgtcgtc
    19141 accctttcat ctaaaccctc gcctctgaag aggcgagcta caaaaggcta tcgcgaatta
    19201 tgagcacaga aaaaaaacgg ctacgcattg gcatcttatt tggcgggcgc tcgggcgagc
    19261 acgaagtctc gttgatttcc gcggcgtcgg tgatttccgc gctcgatccc gaaaaatatg
    19321 aagccgtgcc tattggcatc tccaaagagg gccgatggct cgccggtacg gctgcgcaca
    19381 agatgctgcc gccgttcctg gaaagcgctc acggcaagcc cgctctgggc gaagtcctgc
    19441 gcacgggcga aagcgtgatg ctctccgccg acccgaatgt tgccgcgctc gtacccgcca
    19501 acaattcgcg aacggacgcg ctgcacgtgg acgtcgtgtt ccccgtgttg catggcacgt
    19561 ttggcgaaga cggcgccgtg caagggctgc tcgatcttgc cgggcttcct tacgtcggct
    19621 caggagttct tggctccgcc gtgggcatgg acaaagatgt gcagaagcgc ttgttcctgc
    19681 aggccaaatt gcccgttggc gaatttatga cgatcccgcg ctcgcagtgg gaaaaatcgc
    19741 ccgcgaaagt tctgcgcgaa attcgcaaaa aattccgctt ccccgttttc gtgaaacccg
    19801 cgacgctcgg cagctccgtg ggcatgacca aagcgcatga cgccaaagaa ctggcgaagg
    19861 ccatggactt tgcgggcgaa ttcgctcaaa aaattatggt cgaaaaggaa attcgcggcc
    19921 gcgaaatcga actgtccgtc ttgggtaacg aagatccgaa agcgtcgatc cctggagaaa
    19981 ttatcccgca ccgtgaattt tacgactacg ccgcaaagta tctggaagaa ggcacgcgcc
    20041 tgatgattcc cgcgaagctg accaaagcgc aggtgaaaaa attccaggaa tatgccgtgc
    20101 gcgcgttccg tacgctcgaa tgcctgggca tgtctcgcgt ggatttcttc ctggaaaatc
    20161 gcaccgggaa aattttgctg aatgagatca acaccatccc cggcttcact tccatcagca
    20221 tgtacccaaa gctctgggaa gcttccggtt taccctaccg ccagttgctc gaccgcctga
    20281 tcgaactcgc gctctcgcaa caccgcgagc gccagcgaac gaaatacacg attgaacttc
    20341 ccgctactac cggttcggac gctcttaagt agcacagcca aaattgcctg tatgtttttc
    20401 cgccgttacg tcagcactcg ctattcgcgc aacttctgag caaatcggaa actcagaatt
    20461 gtgctaatcg ccacgcctcc tgcaaataac aatccaagcg tcaaaaaatc atcatgagaa
    20521 acatgaccga tcctgtacaa caaacatcca agcaaaagat tcacgaatgc ccagaccacg
    20581 ttcactacgg gcggagacaa tccttttcct gggggatggg cgaagggggt tggaaagcgg
    20641 tcaccggaaa tgccatggac gaagtgaggt acaaaattgg ccagagtagc gcccgcaaag
    20701 aaacatgcca catagttata ccaactcatg ggctaaatcc tccgggggaa gaaaatatgc
    20761 ggtcaggaat atttgagctg aacgcccagt tcgcgcatca tacattcctc ttttacctga
    20821 gtcaacgaat tcggaacggt ccccgagctt gcccatgcga tcgttcgtcg caggacgccg
    20881 ccgtttgatt gggacagttc cccgggtcct aggctataat gcggcgttcc gaaatacttt
    20941 acttaggatt ttgccgatgc tctcgattcg ccctgccact gtggatgatg ctgcgctcct
    21001 ggccgaaatg atccgcgaat tcgcggcgtt cgagcatctg gagcatgaag tcggcacgac
    21061 ggaagaagac cttttgcgcg atggctttgg tccgcgcccg aaattccgct ccgtgattgc
    21121 cgaatgggaa ggcaagcctg tcggctacgc cgtatttttt gagttctact cctcgtttca
    21181 agggcgcgca ggacttttcc tggatgatct gtacgtgcgg ccggattcac gcaagcacgg
    21241 gatcggcacg gctctgatta agcatgtcgc gggaattgcc tggcggggga aatatttttg
    21301 catgcgttgg gaagttctgg actggaacaa gcccgcgatt gatttctata aaaacctcgg
    21361 cgccgtgttt ctcgatgagt ggaaacccgc gattctcatc ggagacgcac tcgaagccgc
    21421 agccaagggg actgcgtaag ctcaatcctt tcaccacgca gcccgcgaaa ttcttctttc
    21481 tataaattcc ctgcaatatt ttcgctgttc aaacgtcttc atttctagat ggaaaaagta
    21541 ataagtacaa atttgattat ggctgagcaa gaccagaagc tcaccgaaac gatcggccgg
    21601 gaacaaggcc ggctgcggaa ttttattcgc aagcgcgtcc ccgatgaggc cgatgcggaa
    21661 gatattttac aggacgtttt ctacgaattg attgaagcat acaggctgat gaaaccgatc
    21721 gagcaggcag gcgcgtggat gtttcgcgtc gcgcgcaatc ggattgtcga ccgttttcgc
    21781 aagaaaaagc cggaggcatt tactgccctg aaagatccgg acgaagaaag cggcgattca
    21841 ctgacgctcg aagatttgct gccttcgccc gatgcgggcc ctgaggcggc ttacgcgcgc
    21901 agcattttga tggaagagct ggacgcggca atcgatgaat tgcccgaaga ccagcgcgaa
    21961 gtttttattg cgcacgaata tgacggcgtg agttttaaag aaatttcggc cgaaacggga
    22021 gtgagcgtca acacgctctt gtcgcgcaaa catcacgccg tggttcacct gcggcagcgc
    22081 ctgcaaacga tttacgatga atttttgaat ccataaggag aatcgatatg agaggaaaca
    22141 gatttcttcg ggtcttaaaa tttatagcga tcggggccgt ggccttcacg gcgttcggtt
    22201 tcgtggttac gtatttgtgg aattatctaa tgcctggatt gttcgggctt cacgccatca
    22261 cgttctggca ggccatcgga ttgatgctgc tgggacggct gctattcggc ggattccgcc
    22321 cgggccgcgg cggattcggc tggcggggcg gtgaaatgcg ccgtcgctgg gaaaagatga
    22381 cgcccgagga acgcgaaaaa ttccgcgaag gaatgcgcgg ccgttgtggc tcgccgcgga
    22441 caccggcgcc ccaaccgaat cagtagctgc cttcagtggc ataggcttca gcctgtggaa
    22501 tttaggcctt gcaaagtcta aaatccacag gctgaagagt ctgtatgaga actgcagaat
    22561 ctttgtgggt cgtggcttta gccacgacat aagttctgca aaatgaatgc ggctttacag
    22621 tttgcggaaa aagtagttt
//
LOCUS       DASZMB010000255         9807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1079333, whole genome shotgun sequence.
ACCESSION   DASZMB010000255 DASZMB010000000
VERSION     DASZMB010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 9807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9807
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079333"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1292)
                     /locus_tag="VG272_12005"
     CDS             complement(<1..1292)
                     /locus_tag="VG272_12005"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HWF14455.1"
                     /translation="MNKKITFSKNYRAVAAGTIGALLLMSAGLANAAPLPHPAATATT
                     LAQPALPQQPAANDSDKTLAAMQDELDRARTRLELKIPDTNEPARPYFIQYRVLDLDV
                     RTIVAEFGALVSSTAGRNRIMSVDVRVGNKNLDSSNFITDEGFSGFLGSTGTVGIDRD
                     YESLRQDLWLATDQAFKAAVENYSKKQAFLSRLAKAPSIPDFSDAPPTVSVEPLQASD
                     WSTRNWEQEARTASAALRQFPQLYSSRVVYHLIYTTSYLINSDGTKIRANRSLAAIEA
                     SLETESDDGMALHNFYAAYRHRPDQLPAADAVKKDLEKAGEDLISLRVSPPAADYDGP
                     VLFDAPAAGSLLAQMLGPSLSGARGPLAMQTAFDQLMDRLGGRSEWTGKLGSRVLPGS
                     VSLLDDPTTKQFQGRDLLGSYEVDQEGAKAQRVELVNSG"
     gene            complement(1313..3058)
                     /locus_tag="VG272_12010"
     CDS             complement(1313..3058)
                     /locus_tag="VG272_12010"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HWF14456.1"
                     /translation="MKTFCLAVLATFISMNVWAANKPAPAPDAAPSAQKMAQAAPSPL
                     IQTMSQELDREMPILSKATPPAYFVSYILTSSDRSEVMGSNGALLSSEESHSRWLETQ
                     VRVGNYDLDNTHKVGNSGPTQGSFGASVPVDDAPDVLRRAMWAETDKQFRSASEGLIK
                     INTSKEVQVQTLEEHAPDFAREQPHTFYGPEVSVHPDRKPWEQKVRDYTRAFRASPQI
                     LNSIVTFSATGDNQYQVTSEGSRLQFGQVHYRLELFIQGKAPDGMDINRYYNFDWSNP
                     SDAPDDKTVLAEGQVLRKELEALVAAPLVEPYAGPALLTGRATAVFFHEVFGHRAEGF
                     RQKDINEGQTFARKVGEPILPKFLSIYDDPTQRTLGKDILIGFYPFDDEGTPSERVTL
                     VDHGVLKNFEMSRQPLTGFPVSNGHGRRQIGAQPVSRQGNLIVQSTLTVPNAELRKKL
                     VEEITRQGKPFGLLIDDIAGGFTFTGRGQPQAFQVQPLVVYKVFADGRPDQLVRGVDI
                     VGTPLVSLTKIVATGDTMETFNGYCGAESGSVPVSASAPAILITEMEVQKKETSTDKP
                     PILPPPGHDPQAVRK"
     gene            complement(3334..4128)
                     /locus_tag="VG272_12015"
     CDS             complement(3334..4128)
                     /locus_tag="VG272_12015"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HWF14457.1"
                     /translation="MRNRPPFLIALAILISASIIVPPDISAQAAQRAGQISRAIPDVA
                     IARGTQQLPAPVKALVDWGDAVKTGDGGRARVALDDGSVLNVGSSSTLTVTQHNAAAQ
                     QTQIELTYGRMRSQVVKQAKPNSKFEVHTPVGVAGVVGTDFFLGYMNGLFQIIVYEGH
                     VKFCNLDGNCVDVLAGQIATIRDGHQAPDQPAQATPSELTEAANATSVGTSLAGVPPV
                     HHIGLGWIIGLTAVVVIPAIVVPIATRGGGTPVKAGTTCQTNPAFC"
     gene            complement(4329..4880)
                     /locus_tag="VG272_12020"
     CDS             complement(4329..4880)
                     /locus_tag="VG272_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14458.1"
                     /translation="MYVCRQCENEINQATEICPHCGVDLTDPLLSDGPLKPKPTIGKI
                     LLRWGVLLAVLLGAIWSFLWFIVPERRGNPTTLAENRAVESLREINTALGSFASGDGG
                     AYPQQLEAIGEKARLAAQLAQSVNYQIQYTPGPVETDGMIRSFVLQAKAGNYGFRNFY
                     LDNSGILRATREGRAATSEDPPY"
     gene            complement(5113..5607)
                     /locus_tag="VG272_12025"
     CDS             complement(5113..5607)
                     /locus_tag="VG272_12025"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HWF14459.1"
                     /translation="MNQGRNKEKGFSLIELLIVVTIILIIAAIAIPNLMRNKIQANET
                     AAVETMRTLNESILLYSNSYGGFPHALSDMGPASGGTAAAATSAGADLIDVTLASGVK
                     SGYKFAYTAVMTDPAGNVQSYSITATPVVAGSTGQRSFFTDQSGTIRNTSSGTADAAS
                     APIS"
     gene            5824..6816
                     /locus_tag="VG272_12030"
     CDS             5824..6816
                     /locus_tag="VG272_12030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWF14460.1"
                     /translation="MKPDEFMSGEKFLTSLDRFLAQFSQVVTALQEEAKCARWALSPN
                     VFAEALRRSAEKRFGATNPNLGEIEAYLKSLHLEDLALACACGEGEGKAWEYFVAHFR
                     QDLRNAATAILRGSGQASEGRAAELADSLYGELYGVRSSGSAPRKSLFEYFHGRSKLS
                     TWLRAVLAQKHVDLLRTSGKTVSIEDDKKSGSFRDPAMQTEFEAPDPDRELYLGRVAR
                     CLSAALAALTPRERLILACYYVDRLTLAEIGRTLREHESTISRQLERTHRALRENVAQ
                     SLRIGSPARRDCPAEPAMDDAQVELAFEYAVEDWPFDLSQALSASDAIAADPAE"
     gene            6899..8290
                     /locus_tag="VG272_12035"
     CDS             6899..8290
                     /locus_tag="VG272_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14461.1"
                     /translation="MDSKDRDESTNRQRETALARRFGEALDQLNPQNAKDCPDAEVIA
                     AYSEHALDADELARCENHFAACGRCRNVLRVLAAASDAPLAETEVAQLGQRISAVRAP
                     VEITVGAAKRARSKAADWSTRWLAPAFGIAAVLTVWFVMRPPWRSMDRSGSPTLIAQA
                     PREEMPESSPPTAGEQAQKTAPAPEVPAAKIGSPKSNAEPQAKESLKPGNEIRESSPN
                     ANVAENLPSEKKKSTAVAEGNKTKSLAMSTPPAAPPQPIIPSRMNPAVRLPQSSAQAD
                     ASTLAASGASHSPNQAVVVTEAAPQVETTNGTLGGLASQPPGAEVPINGRNFQALAKL
                     RSAQRTPIMMKSTSGTTLWRIGIAGVIERSSDAGKTWTPQISPSKENWLSGAAVSDTV
                     CWVAGSNGAIARTTDGEHWESIAPPAQAAMKSGKLPDWIGITSRDAQSATITANDGTK
                     FVTSDGGKTWQQQ"
     gene            8370..9056
                     /locus_tag="VG272_12040"
     CDS             8370..9056
                     /locus_tag="VG272_12040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14462.1"
                     /translation="MKTATLARFISITGFAVLLIFAPFLHRSASANSAAQTAAQASDK
                     DVTTTLADQTDLALTVYNSNLSLVRDVRQLNIPAGESLLKFMDIAASINPATVHFRSL
                     NDPSKVNVLEQNYEYDLLDPNKLLQKYVGREVTLVRAKFNSGSTEYEELKATLLSFNG
                     SPVWKIGNEIVSGISYESIRFPTLPENLYERPTLLWTLQNGGARSQRVEASYLTANLS
                     WSADYVLNVA"
     gene            9087..>9807
                     /locus_tag="VG272_12045"
     CDS             9087..>9807
                     /locus_tag="VG272_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14463.1"
                     /translation="MTLVNHSGTAFKNAKLQLIAGNLHREVPVNGRMKAADMPMVELT
                     SAGIAPFAQESFSEYHLYSLNRRTSIFDQESKQISLLNASHFPLRKVYVVNGQPYYYR
                     NAYQPGAPIKDPVQVFYKFRNEEKSGLGMPLPSGTIRVYQQDSHGGSLFAGEDHIDHT
                     PKDEEISLLIGDAFDIVAERKQTDYKKVADRLYEFEYEITLRNHKDGPITVEVNEPIG
                     GDWEMVSSTYKFTKTAAFAAQF"
BASE COUNT         2268 a         2716 c         2707 g         2116 t
ORIGIN      
        1 ccgctgttca cgagttccac gcgctgcgct ttcgcgccct cctgatccac ttcgtagctg
       61 cccagcaaat cgcgcccctg aaattgtttc gttgtcggat cgtccagcag cgaaaccgag
      121 ccgggcagaa cgcgtgagcc gagttttccg gtccactcgc tgcgcccgcc gagccgatcc
      181 atcaattgat cgaaagccgt ttgcatcgcc agcggtccgc gcgcgccgga aagggagggc
      241 ccgagcattt gcgcgagcaa agaacctgca gcgggagcat cgaacagcac tggcccgtca
      301 taatctgcgg ctggaggaga aacgcgcaga gagatcaaat cttctcccgc cttttcaaga
      361 tcttttttca ccgcatcggc ggcgggaagt tgatcggggc ggtgacggta ggcggcgtag
      421 aaattgtgca gcgccatgcc atcgtccgat tcggtttcaa ggctagcttc gattgccgcg
      481 aggctgcgat tggctcgaat cttggttcca tcgctgttga tcaggtagct cgtggtgtag
      541 atcaggtgat agacaacacg cgagctgtaa agttgcggga actggcgcaa cgcagcggag
      601 gcggttctgg cttcctgctc ccaattgcgc gtactccagt ccgaagcctg cagcggctcg
      661 acggaaacgg tgggcggcgc gtcagaaaaa tcaggaatgc tgggagcttt cgcgaggcgc
      721 gatagaaacg cctgcttctt ggaataattc tcaaccgcgg ctttgaacgc ctggtcggtg
      781 gcaagccaca aatcctggcg cagcgactcg taatcgcgat cgattccaac cgttccggtc
      841 gagccgagga aaccgctgaa gccttcatca gtaataaaat tcgagctgtc cagattttta
      901 tttccaacgc gtacatcgac gctcatgatg cgattacgac cagcggttga ggaaaccagc
      961 gcgccaaatt ccgcgactat tgtgcgcacg tccagatcga gaacgcgata ttgaataaaa
     1021 tacggacgcg caggctcatt cgtatccgga atttttaatt ccaggcgcgt gcgcgcgcgg
     1081 tccagttcat cctgcatggc cgcgagtgtt ttgtccgaat catttgccgc gggttgctgc
     1141 ggcagggccg gttgcgccaa tgttgttgca gttgccgcgg gatgcggcag tggtgcagca
     1201 ttggcgagtc cggcggacat taaaagaaga gcgccaatag tgcccgcggc aacagcacga
     1261 taatttttag aaaatgtaat ttttttgttc atcaggttcc ccaaggaaat attcacttgc
     1321 gcaccgcctg cggatcatgt cccggcggcg gcaaaatcgg cggcttgtcg gtggacgttt
     1381 cctttttctg aacttccatc tctgtaatta aaatcgcggg agcggatgcg gaaaccggta
     1441 cggaacccga ttccgcgccg cagtacccat tgaaagtttc catcgtgtcg ccggtagcca
     1501 caatcttcgt gagcgaaacg agcggcgtgc cgacaatgtc aacgccgcgc acaagttgat
     1561 cgggacggcc gtccgcgaaa actttgtaaa cgacgagtgg ttgcacctga aacgcctgtg
     1621 gctgtccgcg tccggtgaac gtaaatccgc cggcgatatc gtcaatcaaa agtccgaacg
     1681 gttttccctg gcgagtgatt tcctcgacaa gttttttgcg taattccgca ttcggtacgg
     1741 tgagggtgct ctgcacaatt aaatttccct ggcgagaaac gggctgcgcg ccgatctgcc
     1801 tgcgcccgtg tccgttggaa accgggaaac cggtgagtgg ctggcgcgac atctcaaaat
     1861 ttttcagcac gccgtgatca acgagcgtaa cgcgctcgga gggagtgcct tcatcgtcga
     1921 acggatagaa tccgatgaga atgtccttcc cgagagtcct ttgtgtcgga tcgtcataga
     1981 tcgaaagaaa tttcggcaga atgggttcgc ccacttttcg cgcgaacgtc tgcccttcgt
     2041 tgatgtcttt ctgtcggaac ccctccgcgc gatggccgaa aacttcgtgg aagaaaacgg
     2101 cggtcgcacg gccggtcagc aatgcggggc ccgcgtaggg ctcaaccagc ggcgcagcaa
     2161 caagcgcttc gagctccttg cgcagcactt gtccctcggc gagcaccgtc ttgtcgtccg
     2221 gcgcgtcaga aggattcgac cagtcaaaat tataatagcg attaatgtcc atgccgtccg
     2281 gcgcttttcc ctgaataaac aattccagac ggtagtgaac ctgaccgaac tggagccgtg
     2341 agccttcgct cgtaacctgg tattggttgt cgcccgtggc gctgaacgtg acaatggaat
     2401 tcaggatttg cggcgaggcg cgaaatgcac gcgtgtaatc gcgaactttt tgttcccagg
     2461 gcttgcggtc gggatgcaca gaaacctcag ggccgtaaaa agtgtgtggc tgctcgcgcg
     2521 caaaatcggg ggcatgttcc tcgagcgttt gcacctgcac ttccttgctc gtattaattt
     2581 tgatcaggcc ttccgaagcg gagcgaaatt gcttgtccgt ctccgcccac atcgcgcggc
     2641 gcaaaacgtc gggagcatcg tcgaccggca cggaagcgcc gaaacttcct tgcgtcggcc
     2701 cggagttgcc aactttgtgc gtgttatcca gatcgtagtt tccgacgcgc acttgcgttt
     2761 ccagccatcg cgaatggctt tcctcgctcg atagcagcgc gccgttcgag cccatgactt
     2821 cggagcggtc cgaggaagtc aaaatgtagc tgacgaaata ggcgggcgga gttgccttgg
     2881 aaagtatggg catctcgcgg tccaattcct ggctcatcgt ttgaatcagg ggagaaggcg
     2941 cagcctgagc catcttctgg gcagagggcg ctgcgtcggg ggcgggagca ggcttattag
     3001 ccgcccacac attcatggaa ataaaagtag caagtaccgc aagacagaat gttttcacag
     3061 cactactcca ttctactaaa agggaattca agagattaag ttactaagtc tatgcttatg
     3121 ttcgaccgat ctcaatgcgc gtgttttttg ggcgttcttt cagcaggcga aacataaacc
     3181 aacccaccgg tattagatgc aggaaaggcg tttcgcgtag ccgcacaaac ccacgcaggt
     3241 tagacgcatg gaaatggaag ttagctctaa aaaaataccg gaggctcctc ggtcgagcct
     3301 ccgtgcgata cgtttgccgc tcggcagctc aggtcagcaa aaagcaggat tggtttggca
     3361 ggttgttccg gctttcacag gcgtgccacc gccgcgggta gcgatcggca ccacgatcgc
     3421 tggaatcacg acaaccgccg tcaacccgat aatccatcca agaccgatat gatgtactgg
     3481 aggcaccccg gcaagggacg ttcccacgct cgttgcgttg gccgcttcag tcaactccga
     3541 aggcgtggct tgcgcaggct ggtcgggagc ctgatggccg tcacgtatcg tcgcaatctg
     3601 ccctgcgaga acgtccacgc aattgccgtc caggttgcag aatttcacgt ggccttcgta
     3661 cacaatgatc tgaaaaagac cgttcatgta gccaagaaaa aaatcagtgc ccacgacgcc
     3721 cgcaactccc accggagtat gaacttcaaa ttttgaattc ggcttggcct gcttgaccac
     3781 ctgcgagcgc atgcgcccgt aggtcagctc aatctgcgtc tgctgcgcgg cggcattgtg
     3841 ttgcgtcacg gtcaatgtgg agctcgagcc gacattcagc acagaaccgt catcgagcgc
     3901 aacgcgcgcg cgtcctccgt cgcctgtctt gacggcgtct ccccaatcga ccagcgcctt
     3961 gaccggcgcg ggaagttgct gcgtgccgcg cgcaatcgcc acatcaggaa tcgcgcgtga
     4021 aatttgcccg gcccgctgcg cagcctgagc cgaaatatcc ggcggcacga tgatcgaggc
     4081 agaaatcaga attgcgagcg cgattaaaaa tgggggacga ttcctcataa attctcctga
     4141 aaaaatctat tcacactgag ccgcagccat ccttagttac tggataggtt cttcagtgtc
     4201 aacgcgatcg atacaaaaac gtaccgaatt tgacaatggg gaaagggtga aacgaatcgt
     4261 ttcgatcctg aagcggtcga catttacaag aatatttgga agttatttaa ggatctaaag
     4321 gaagatgctt aatagggcgg gtcctcggaa gtcgctgcgc ggccttccct cgtggcgcga
     4381 agaattcccg agttgtccaa ataaaaattc cggaagccgt agtttcccgc cttagcctgc
     4441 aacacaaaag agcgaatcat tccgtcggtt tccacggggc ctggagtgta ttggatttgg
     4501 taatttacgc tctgcgccag ttgcgctgcc agccgcgctt tttcaccaat cgcttccagt
     4561 tgttgcggat aagcgccgcc atctcccgaa gcgaaactgc cgagcgctgt attgatttcg
     4621 cgcagggact ccactgcccg gttttccgcc agcgtcgtgg gattgcctcg ccgctcaggc
     4681 acaatgaacc acagaaaact ccaaatcgct ccgagcagca ctgcgagcag aacgccccag
     4741 cgcagcagga ttttcccgat ggttggtttt ggcttaaggg gtccatcgct aagcaacgga
     4801 tcagtcaggt cgacaccgca atgcgggcaa atttctgtcg cctgattgat ttcattctcg
     4861 cattggcgac aaacatacat attttttgcg ctagtcgaag aagaaggcat ggacgcatcg
     4921 ggcagcgcgc gagcgcgtca attccccaca tcctatccaa gggtcaggct aagagtcaat
     4981 tgtctctgcg gacgaacgcc tttcagtagc acagacttta gcctgtggat tttcgtcctt
     5041 gcaaggacaa aaccccacag gctgaagcct gtgctattca atcctgcagc gcattgaaaa
     5101 tatttatatg ggttagctga tgggcgcgct ggcagcatcc gctgtgccgc tggaagtgtt
     5161 tcgaatcgtc ccgctttgat cggtgaagaa agatcgctgt ccggtcgatc ccgccaccac
     5221 cggcgtggcg gtaatcgagt aactctgcac atttccagca ggatctgtca ttacagccgt
     5281 ataagcgaat ttgtagccgc ttttcacgcc gcttgcgagc gtcacatcga tcagatcggc
     5341 gccggctgac gttgctgccg ccgccgtccc gccggaagct ggccccatat cggaaagggc
     5401 atgggggaat ccgccgtagg aattcgaata caggagaatg ctctcgttga gagtcctcat
     5461 cgtttcgacc gctgcggttt cattcgcctg gatcttattt cgcatcagat tgggaatagc
     5521 gatggcagcg atgatcaaaa tgattgtcac aacgatcagc aattcgatca gcgaaaatcc
     5581 cttttccttg tttcggcctt ggttcataac atctcctgtc caatctggca aaaattcccg
     5641 ggaatccggt ttgcgattgt accgttttcc tggcaaacac cggagcgagt gaagagtccg
     5701 ctttgcaccc tgattgaaac gctattctac accggtcagg gaattatggg gtgaccagaa
     5761 agtacttttt cagccacacc cgcagcctgt acgtactagg tcggctatga tgctacaaat
     5821 caagtgaagc cagatgaatt tatgtccgga gaaaaatttt taacgtctct agatcgcttt
     5881 ctcgcgcaat tctcgcaggt cgtaaccgcc ttacaggaag aagcgaagtg cgcgcgctgg
     5941 gcgttatcgc caaacgtttt tgccgaagct ttgcggcgca gcgctgaaaa acgatttggt
     6001 gcaacgaatc cgaatctcgg tgaaatcgaa gcgtacctga agtcgcttca tcttgaggac
     6061 ttggcgcttg cctgcgcatg cggcgagggc gaaggaaaag cctgggaata tttcgttgcc
     6121 cattttcgcc aggacctgcg taatgccgcg accgcgatac tccgcggatc tggacaagcc
     6181 agcgaaggcc gcgccgcaga gttggcggac tctctctatg gcgaactgta cggcgtccgg
     6241 tccagcggat cagctccgcg gaaatctctg ttcgaatact ttcacggtcg cagcaaactt
     6301 tccacgtggc ttcgcgccgt gctggcgcag aaacatgtag acctgcttcg aacaagcggg
     6361 aaaacagttt cgatcgagga cgacaagaaa agcggctctt tccgcgatcc tgcaatgcaa
     6421 actgaattcg aagcgcccga tcctgaccgc gaactttatc tggggcgcgt cgccagatgt
     6481 ctttctgcgg cgttggcagc cttaacgccg cgcgaacgct tgattctggc gtgttactac
     6541 gtcgaccggc tcacgcttgc ggaaatcggg cgcacgctcc gcgaacatga atcgacgata
     6601 tcccgtcagc tcgaacgcac gcaccgcgcg ttgcgcgaaa atgtcgccca atccctacgg
     6661 attggcagcc ctgcgcgcag agattgtccc gcggagcctg cgatggacga cgcgcaggtg
     6721 gaactggcgt tcgaatacgc agtggaagac tggccgttcg acctatcgca ggcgctttcc
     6781 gcttccgacg cgattgcagc ggacccggcg gagtgaagtc cgttggcatt tggcgagtat
     6841 tcgacacgct gcaagatttt tgagttaaac cgttctaaag taaggaagag ttttctttat
     6901 ggactcaaaa gacagggacg aatccaccaa tcggcaacgg gaaacggcgc tcgcgcggcg
     6961 ttttggcgaa gctctcgacc agctaaatcc ccaaaacgct aaggactgcc ctgacgcgga
     7021 agtcatcgct gcatattcgg agcacgccct tgacgcggac gaattagcgc gatgtgaaaa
     7081 ccattttgcg gcgtgcggcc gttgcagaaa tgttttgcgc gtgctcgccg ctgcatcgga
     7141 cgcgccactc gctgaaacgg aagtggccca gcttgggcaa cggatctctg ccgttcgcgc
     7201 cccggtagaa atcacggtag gcgccgcgaa acgcgcacgt tcaaaagcag ccgattggag
     7261 cacgcgctgg ctcgcccctg ccttcgggat tgccgccgtc ttgactgtct ggttcgtcat
     7321 gcgtcctccg tggcgcagca tggatcgcag cggatccccc acgttgatcg cgcaagcgcc
     7381 tcgggaagaa atgccagaaa gttcgccgcc cactgctggg gagcaggcgc agaaaactgc
     7441 tcctgcgccg gaagttcctg ctgcaaaaat tggctcgccg aaatcaaacg cggagccgca
     7501 ggccaaggaa agtttgaaac cggggaacga aattcgggag agttctccaa acgccaatgt
     7561 tgctgaaaat ttgcccagtg agaaaaagaa atccaccgcg gtcgcggagg ggaataaaac
     7621 aaaatcattg gcgatgtcta cgccgccggc tgcgccccca caacccatca tcccatcaag
     7681 aatgaatcca gcggttcgtt tgccgcaatc ctccgctcaa gcggatgcat caactctcgc
     7741 tgcatctggg gcatctcatt cgccgaacca agccgtcgtc gtgactgagg cggctccgca
     7801 ggtcgaaaca acaaatggaa ccctcggggg attagcatct caaccgccgg gtgcagaggt
     7861 tcccatcaat gggagaaatt ttcaagcgct tgctaaattg cgttccgctc aacgaactcc
     7921 aatcatgatg aaatccacgt ccggaaccac actctggcgc atcggcatag ccggagttat
     7981 cgagcgttcc agtgacgcag gaaagacttg gaccccacaa attagtccat ccaaagaaaa
     8041 ctggctttct ggtgccgcgg tttctgacac ggtatgctgg gtggcaggca gcaacggtgc
     8101 catcgcgcga accacggacg gagagcactg ggaaagcatt gccccgcctg cgcaggctgc
     8161 aatgaaaagc ggcaaattgc cggactggat cggcatcacg tcacgcgacg cccaatccgc
     8221 gacaattacc gctaacgacg gcactaaatt cgtcacctcc gacggcggaa aaacctggca
     8281 gcaacaatag agaaataacg tgcaagaaat tcgggcagtc ccgttctaag agttgaagga
     8341 agaattcgat ctcttaacgg gagacgccca tgaaaaccgc cacgctagcc agattcattt
     8401 ccattacagg atttgcagtg ctgctgatct tcgcgccgtt cctgcacaga tcggcatccg
     8461 caaactccgc agcgcagact gcggcgcagg cctcggacaa agacgttacg accacgctcg
     8521 cggatcagac ggaccttgcg ctgaccgttt acaactcgaa tctgtctctc gtgcgcgacg
     8581 tgcgtcagct caacattccc gccggggaat cgctcctgaa attcatggac atcgcagcgt
     8641 ccatcaatcc ggccaccgtg cattttcgtt cgctgaatga tccctccaaa gtgaatgtgc
     8701 tggagcagaa ctacgaatac gatttactcg acccgaacaa gttgctgcaa aaatatgtag
     8761 ggcgtgaagt cacactcgtg cgcgcgaaat tcaatagtgg ctcgacggag tatgaggaat
     8821 tgaaggctac gctgctttcg ttcaacggtt cgccagtttg gaaaattgga aatgaaattg
     8881 tttcgggaat ttcttatgaa agcatccgat ttcccacatt gccggaaaac ttatacgaac
     8941 gccctacgct cctgtggacg ctgcaaaacg gtggcgcgcg aagccagcgc gtggaagcgt
     9001 cctatctgac cgcaaatctt tcctggagcg cggattatgt tttgaatgtt gcgtaggatg
     9061 atgcatttgc cgatctcgat ggctgggtga cgctcgtcaa tcacagcgga acggcattca
     9121 agaatgcaaa gcttcagcta atcgctggca acttgcaccg ggaagttccg gtcaacggac
     9181 gaatgaaggc cgcggatatg ccaatggtag aactaacgtc ggcgggaatt gcacctttcg
     9241 cgcaggaatc gttttccgaa tatcacctat actcgttgaa tcgccgcaca tcgatcttcg
     9301 atcaggaatc caagcaaatc agcttgctga acgcttccca ttttccgctg cgcaaagtgt
     9361 acgtggtgaa tggacagccc tactactatc gcaacgcgta tcaacccggc gcgccgatca
     9421 aggatcctgt gcaggttttt tacaaattta ggaacgaaga aaagtccggg ctcggaatgc
     9481 cgctgccgtc gggcacgatt cgtgtgtatc agcaggattc gcacggcggc tcgctctttg
     9541 ccggagaaga tcacattgac cacacgccaa aagacgaaga aatcagcctg ctcatcggcg
     9601 acgcattcga catcgtggct gagcgcaagc agacggatta caaaaaagtc gctgaccgcc
     9661 tctatgaatt tgaatacgaa atcacgttgc gcaatcacaa agacggaccg atcactgtcg
     9721 aagtgaatga acccatcggc ggagattggg aaatggtcag ctcgacttac aaatttacta
     9781 agaccgctgc gtttgccgct caattcg
//
LOCUS       DASZMB010000256         3504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_253038, whole genome shotgun sequence.
ACCESSION   DASZMB010000256 DASZMB010000000
VERSION     DASZMB010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3504
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253038"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="VG272_12050"
     CDS             <1..1278
                     /locus_tag="VG272_12050"
                     /inference="COORDINATES: protein motif:HMM:NF024924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWF14464.1"
                     /translation="PDDDGSFAKAGLRCIGLGECRKQDSGSMCPSYMVTLEEEHSTRG
                     RSHMLFEVLQGEVIDDGWKDENVKKALDLCLSCKACKSECPTNVDVATYKAEFLAHYY
                     EENRRPLHAYAFGMIDRWAKLASHTPRLANFFSSAPIFKQILQGLLHLAPQRKMPQFA
                     TATFQSWAKKHKVADIRGSASAEHANGKGDIILWADTFNNYFHPDTSRAALEVLESAG
                     FNVMVPQIHLCCGRPLYDFGLLNEAKEYLEKVMTVLTKQIVAGVPLVVLEPSCASVFR
                     DELRNLFPHDDRATKLRSQTFLLSEFLERYAPEFAPQLSAKILLHGHCHHKSIMGIGA
                     EESVLKKIGADLQSIDSGCCGMAGPFGFEKEKYEVSQAVGERVLLPAVRNTPENALIV
                     SDGFSCREQIHQATGRRALHLAEALQLGLKNKK"
     gene            1372..2451
                     /locus_tag="VG272_12055"
     CDS             1372..2451
                     /locus_tag="VG272_12055"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14465.1"
                     /translation="MNIEKVLRSKILLIASVATLLIGTRPANAQAPKYEIDPTWPKPL
                     PGLMVTGRAGGICVDAQDHVFEVNRGDLQEKEKLVGTPAPPVLEYDSDGNLVNTFGDF
                     KTAPASIHGCILDKDNNIYVAGNDDAVVQKYTHDGSKMLLTIGTKGLFDSSTGLITGI
                     AMNRSKTLLNKPADIAVDPSNGDIYIADGYGDHRIVVFDKDGKFLRQFGEQATAAEST
                     AGEGGKFMGVVHCVVMDNAGLVYVCDRQGNRIQVFDKMGNFKKNIWIKGYKEETPDSW
                     GTAWWLRFSRDPEQKYMIVADGRNELVHILDHKTGNEITKFGRPGHQLGEFTHCHTLD
                     IDSKGNIYVAETDIGRRVDKFKLVK"
     gene            2542..>3504
                     /locus_tag="VG272_12060"
     CDS             2542..>3504
                     /locus_tag="VG272_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14466.1"
                     /translation="MKKIIFILVLAIVFAVQSPAQDKAPLKLVQTITAPAGRKWDHFG
                     VDLKGNRLFVASEEEPAVEVFNLKTGEHIRSLTDFKEPHNVLPFPELKKIFVVDGEAS
                     EIKILDYDSYKLIGQTALTIDADPVVYDPASKYLYVVNGGREAHTPTCLISIVDTVTG
                     KKIADMKLDTNRLESMVIEKSGQRLFVNMTGINSIGVVDREKRAVVQTWPITAGKDNV
                     PLQYDEATHRLFLATRKPSKLVVVNADNGKEVASLDVADYVDDLAYDAAHHRLYIPGG
                     GGNGAVSVVEQRGADDYKLIATVPTKPGAKTARLVPELNKYYVGV"
BASE COUNT          863 a          865 c         1003 g          773 t
ORIGIN      
        1 ccggatgacg acggttcttt cgccaaggct ggattgcggt gcatcgggct gggcgaatgc
       61 cgcaagcagg acagcggctc gatgtgcccc agctacatgg tgacgctcga ggaagaacac
      121 agcacgcgcg ggcgctcaca catgctcttc gaggttttgc agggcgaggt gattgacgat
      181 ggatggaagg acgaaaacgt caaaaaagcg ctcgacctat gcctttcctg caaggcgtgc
      241 aaatcggagt gcccgacgaa tgtggatgtg gccacgtaca aggctgaatt tctggcgcat
      301 tactatgagg aaaaccgccg gccgctgcac gcgtatgcgt ttggcatgat cgaccgctgg
      361 gcaaagctcg catcgcacac accaaggctt gcaaattttt tcagcagcgc gccaattttt
      421 aagcaaatct tgcagggcct tttgcattta gcgccgcagc gaaaaatgcc ccagtttgcg
      481 accgctactt ttcaaagctg ggcgaagaaa cacaaagtgg ctgacatcag ggggtcggcg
      541 tccgcagagc acgcgaacgg aaaaggcgac atcattttgt gggcggacac gttcaacaat
      601 tatttccatc cggatacgag ccgcgcggcg ctggaagttc ttgaaagcgc cggattcaac
      661 gtgatggttc cgcagattca tttgtgctgc gggaggcccc tgtacgattt tggtttgctg
      721 aacgaagcca aggaatatct tgaaaaagtt atgacagtgc tgaccaagca aattgtggcc
      781 ggcgttcctc tggtggtgct ggagccaagt tgcgcttccg tattccgcga tgaactgcgc
      841 aatttgtttc cgcacgatga tcgagcgacc aagctgcgca gccagacgtt tctgctcagc
      901 gaattcctgg agcgctatgc tcctgaattt gcgccgcagc tttccgcgaa aattttgctg
      961 catgggcatt gccaccacaa gtcgatcatg ggcatcggcg cggaagaatc cgtgctgaag
     1021 aaaatcgggg cggacctgca atcgattgat tccggctgct gcggaatggc ggggccattt
     1081 ggttttgaga aagaaaagta tgaagtgtcg caagcggtgg gcgagcgggt tttgttgccg
     1141 gccgtgagga atacacccga gaatgcgctc atcgtatccg acgggtttag ttgtcgagag
     1201 cagattcatc aggctacggg acgccgcgcg ctgcacctgg cggaagcgtt gcaacttggg
     1261 ttgaaaaaca agaagtaagg gatgaaaaag aacgcggatt gatttttaga attcgcgctt
     1321 tgcaacggtc ggctataaat attcagtggc gaattatcca ggagacgata aatgaatata
     1381 gagaaagtgt tgcgaagcaa aatcctgctt attgcaagcg tggcaaccct tttgatcggg
     1441 acccgcccgg cgaatgctca ggcgccaaag tacgaaatcg atcccacatg gccgaagccg
     1501 ctacccgggc tcatggtcac cgggcgcgct ggcggaattt gcgtcgacgc gcaggatcat
     1561 gttttcgaag tgaatcgcgg cgacttgcag gaaaaagaaa aactcgtcgg cacgccggca
     1621 cctcccgtgc tcgaatatga ctccgatggg aatctggtta acacattcgg tgatttcaag
     1681 accgcgcccg cttccatcca cggatgtatt ttggataagg ataacaacat ttacgttgcc
     1741 ggcaatgacg acgccgtggt gcaaaaatat acgcatgacg gcagcaaaat gcttttgacg
     1801 attggcacca agggtctttt cgacagctcc acgggcctga tcaccggaat cgccatgaac
     1861 cgcagcaaga cgctgctgaa caagcccgcg gatattgcag tcgatccttc caatggtgac
     1921 atctacattg ctgacggcta cggcgatcat cgcatcgtcg tgttcgacaa agacggaaaa
     1981 ttcttgcggc agtttggcga gcaagccacg gcggcggaat ccacggcagg cgaaggcggc
     2041 aagttcatgg gcgtcgtgca ttgcgtcgta atggacaatg ccggtctcgt ttacgtttgc
     2101 gatcgccagg gcaatcgcat tcaggtgttc gacaagatgg gcaattttaa aaagaacatc
     2161 tggatcaagg gatacaagga agaaacgcct gactcgtggg gcactgcatg gtggctgcgc
     2221 ttttcgcgcg accctgagca gaaatacatg atcgtggcag atggccgtaa cgagctcgtt
     2281 cacattctcg atcacaagac cggaaatgaa attacgaagt tcggcaggcc cggccatcaa
     2341 cttggcgaat tcacgcattg ccacacgctg gatatcgatt ccaagggaaa tatctacgtc
     2401 gctgaaaccg atattggcag gcgcgtggat aaatttaaat tagtgaagta gtcctgatgg
     2461 catctgacaa tgcgggcggc ggcaatttca tcacgccgcc tcatcttcta aggacgagct
     2521 tccatccgtc ggaggattgg aatgaaaaaa attattttta ttctggtgtt ggcgattgta
     2581 tttgcagtgc aatccccggc tcaagataag gcgccgctta agctggtcca gacgattacc
     2641 gcgccagctg gtcgaaagtg ggaccatttc ggagtggacc ttaaagggaa ccgccttttt
     2701 gtcgcttcgg aagaagaacc cgccgttgag gttttcaatc tgaaaacggg cgagcacatt
     2761 cgctctctta ccgatttcaa ggaaccccac aatgttttgc cttttcctga gttgaaaaaa
     2821 atatttgtcg tcgatggcga ggcctccgag atcaaaattc tggattacga ttcttacaaa
     2881 ttgatcgggc agacggcatt gacgattgac gccgatccgg tagtctacga tcccgcttct
     2941 aaatatctct atgtggtgaa cggcggccgc gaagcgcaca cgccgacctg ccttatcagc
     3001 attgtggata ctgtgaccgg caaaaaaatt gccgatatga agctggacac gaaccgtctg
     3061 gaatccatgg tcatcgaaaa atcggggcag cgtcttttcg tgaatatgac cggcattaat
     3121 tccatcggcg ttgtggaccg cgaaaagcgc gccgttgtgc aaacgtggcc aatcaccgcc
     3181 ggtaaagaca atgtcccgct gcaatatgac gaagcgacgc atcgtttatt tctggcgaca
     3241 cgaaaacctt ccaagctcgt ggtggtgaat gcggataacg ggaaggaagt tgcgagcctg
     3301 gacgtggccg attatgtcga cgatctcgcc tatgacgcgg cgcaccatcg cctgtatatt
     3361 ccgggcggag gcggcaatgg cgccgtgtcc gtggttgagc agcgcggcgc ggatgattat
     3421 aaattaattg caacggtgcc gacgaaaccc ggcgcaaaga cggctcgcct cgtgccggag
     3481 ctgaataaat attacgtggg tgtt
//
LOCUS       DASZMB010000257        14621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2730024, whole genome shotgun sequence.
ACCESSION   DASZMB010000257 DASZMB010000000
VERSION     DASZMB010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 14621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14621
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2730024"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..417
                     /locus_tag="VG272_12065"
     CDS             163..417
                     /locus_tag="VG272_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14467.1"
                     /translation="MKKILLRLWKEQVAQDLVEYALLLVLVSLVLVASLRTLGVGVTK
                     VYSGAATNLAVTGGGGSGGDGGDGGGGDGGGDGGGGRGGR"
     gene            complement(630..899)
                     /locus_tag="VG272_12070"
     CDS             complement(630..899)
                     /locus_tag="VG272_12070"
                     /inference="COORDINATES: protein motif:HMM:NF018229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HWF14468.1"
                     /translation="MVGCMSEEVDAAFKREKRTILVVVNSICGCAAGKARPAVAKAVS
                     HSVLPEILATVFAGGRESSANLFRRLHAVFPIRLRSDEREPAFNQ"
     gene            1386..2660
                     /gene="tig"
                     /locus_tag="VG272_12075"
     CDS             1386..2660
                     /gene="tig"
                     /locus_tag="VG272_12075"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HWF14469.1"
                     /translation="MSEATCRRELDLEIPAETVQKAVKRVSKEFARVARVPGFRPGKA
                     PITLIQRRFADDIKSEVLQSLVPEQVERAVTESKLVPVTQPQIDKVDFSENGPVKFRA
                     VFEVLPEFELGQYKDLEVEVEDLKVEDADVDKGLEELRDRAATFVPVEGRAIADGDYA
                     QLKLNGIPMGGGEPLEAESVLCHVGGEETMEPFNQNLRGASTSDKKSFDVTYPADYPD
                     AKLQGKTYTYAVEVLGIKEKKRPELTDEFAKDVSDAQTLDELRKKMRDNLEAARTHRQ
                     NEQHREKLLAQIVKGHDFPVPEALVEHQMDSRLERTVRSLASQGVDPRAMNVDWVALR
                     NRQKDRSIEDVKAELLLDRIATAEKIEVSDEEVDKEIAALAERSGESATAVRANLTRQ
                     GALDRMKSKIRSEKTLDWLCSNSRTRTITKEN"
     gene            2728..3330
                     /gene="clpP"
                     /locus_tag="VG272_12080"
     CDS             2728..3330
                     /gene="clpP"
                     /locus_tag="VG272_12080"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: protein motif:HMM:NF009205.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HWF14470.1"
                     /translation="MTPYATTLVPMVVEQTNRGERAYDIYSRLLKDNIIFIGTPIDDH
                     VANLVTAQLLFLEAEDPEKDISLYINSPGGSITAGMAIYDTMQFVRPSVVTICVGQAA
                     SIAALLLTAGAPKKRFSLPNSRILIHQPWMSGLSGQATDIDIHAKEILRMRTVINGLL
                     AQHSGHPLDTLEKDVERDFIMSAAQAKEYGLIDEIITKHR"
     gene            3446..4696
                     /gene="clpX"
                     /locus_tag="VG272_12085"
     CDS             3446..4696
                     /gene="clpX"
                     /locus_tag="VG272_12085"
                     /inference="COORDINATES: protein motif:HMM:NF003745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HWF14471.1"
                     /translation="MKKLSTDEPLRCSFCHKTQEQVEKLISSPSDYPRAYICNECVTV
                     CEQILEEEKREQVNPVNKRLPRPPEIKAFLDGYVIGQDKTKKKLAVAVYNHYKRIFLN
                     RQPGDVELTKSNILLIGPTGTGKTLLAQTLSRMLDVPFAIVDATTLTEAGYVGEDVEN
                     IILKLLQAADGDVTKAQQGIIYIDEIDKISKKDENPSITRDVSGEGVQQALLKILEGT
                     IANVPPQGGRKHPHQEFTPVDTTNILFICGGAFVGLEKTIERRIGQKSLGFHTDAQPT
                     TAQRRNIELLSQAQPTDLIRYGLIPEFVGRLPVVGVLGDLDKAALMQILTEPKNALIR
                     QYQRLFEFENVRLRFSDDALETVAELALQRKVGARGLRMILEDLMLELMYHLPAQRKL
                     REFVVTGEMVRSREINWSLLEKAG"
     gene            4693..7134
                     /gene="lon"
                     /locus_tag="VG272_12090"
     CDS             4693..7134
                     /gene="lon"
                     /locus_tag="VG272_12090"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:NF008053.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HWF14472.1"
                     /translation="MMKEQEKITEPMFNKDKSDSKRVPMMPVRDLVIFPQMMNPFIVG
                     RETSLRALEEALATDKKIFLATQHDASVDDPKPDEIFSVGTLANIVQSVRLPDGNLRV
                     LVEGVERAKALQISTDEGFFRATLKVIPNKAEPSPQIEQAVTKVTGLFEQYVKLSQSL
                     NYDTMIAATRVDDPAKLSDTIASNLQIPVEEKQELLENFDPLDRLTRVGEILESEIEK
                     LNVDRNINTRVKRQMERAQKEYYLNEKLKAIHKELGRGEKNELDELKKKIETSGMPKD
                     AMEKATQELKRLEMMPPMSAESTVSRNYLDWLLAVPWKKRSKEIRDIALAEKILEEEH
                     YGLDKVKERILEFLAVRQLVKNPRGSILCFVGPPGVGKTSLATSIGRATGRKFIRVSL
                     GGVRDEAEIRGHRRTYIGALPGQIIQMMKKAGTVNPIFVLDEVDKMSTDFRGDPSAAL
                     MEVLDPELNNAFTDHYLDVEYDLSKVMFVCTANVLHTIPQPLQDRMEILRIPGYTEAE
                     KAQIAKRFLVRKARETTGLQESNLTFTDEAITHIIRHFTYEAGVRSLEREISSICRKV
                     ARRVVKDGKQYSINITPEIVGEFLGVIKYREVWAEKKNEIGLTTGLAWTEVGGSVLST
                     EATIMEGKGRLTLTGKLGDVMQESAQAAMSYVRSRTATFGLPRDFYRHIDIHMHVPEG
                     AIPKDGPSAGITIATSIVSALTRIPVRCDVAMTGEITLRGKVLPIGGMKEKLLAAHRV
                     GIRTVILPKDNEKDLADIPAEIQADLTLRFVENVDQVLEIALERPLTPATHDAGPEVA
                     AKFGTDTESDQELTN"
     gene            7358..8617
                     /gene="rho"
                     /locus_tag="VG272_12095"
     CDS             7358..8617
                     /gene="rho"
                     /locus_tag="VG272_12095"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HWF14473.1"
                     /translation="MSISLAELKEKNITDLAKIAKDLAIPGSSGMRKQELIFQILRAQ
                     TEKNGLIFSEGVLECLPDGFGFLRAPEYNYLPGPDDIYVSPSQIRKFDLRTGDTVSGQ
                     VRPPKDGERYFALIKVEAVNFEDPEVARNKIFFDNLTPLYPQGRLKMETTRENLTGRV
                     LDLLCPVGKGQRGLIVAPPRTGKTMMLQAFANSVTANHPEVALIVLLIDERPEEVTDM
                     QRTVKGEVISSTFDEPPQRHIQVAEMVLEKAKRLVEHKRDVVILLDSVTRLARAYNAV
                     TPPSGKVLSGGIDANALQRPRRFFAAARNVEEGGSLTIIATALIDTGSRMDDVIFEEF
                     KGTGNMEINLDRRLVDKRIFPAIDINRSGTRKEELLMPPDELNRVWVLRKVLNPLSPV
                     EAMELLLGRLSKSKSNAEFLASMSSPT"
     gene            8705..9931
                     /locus_tag="VG272_12100"
     CDS             8705..9931
                     /locus_tag="VG272_12100"
                     /inference="COORDINATES: protein motif:HMM:NF020252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HWF14474.1"
                     /translation="MKYLARQPILNRERELFGYELLFRNGIQNSCEGLDLEMASTSVL
                     DTSFLIGLEKISAGHPMFFNCPREFLIRDYVSLFPPKSIVVEILETIHPDEEIIDACK
                     RLKERGYKIALDDFVDSAKWAPLVALADIIKVDFRLTGITEQREIVSRYASKKIKMLA
                     EKVETQQEFAAGMQMGYSLFQGYFFCHPEMMSHRGLPSFKLTYVELLRAATAPEFNIQ
                     ELAVKIKYEPSLTFRLLRYLNSAFFGLRGEIHSVQHALSLLGERELRKWIAVVSVGVL
                     AEGKPDELMTVPLIRSRFCELLARLAHIPGDANDLFLMGLLSLMDAILDQPIESILKE
                     MPVRGEIKDALLHQKGLYWQLLEITIAHEQADWAKVSRLVSAIGMNDEQVSALYISAV
                     DWCTALRQSIFVPATR"
     gene            complement(10042..10728)
                     /gene="cmk"
                     /locus_tag="VG272_12105"
     CDS             complement(10042..10728)
                     /gene="cmk"
                     /locus_tag="VG272_12105"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HWF14475.1"
                     /translation="MNDRLIIAIDGPVGSGKSTVARRVAALLGYAHLDSGAMYRAVGL
                     KALRGGVPLDSPERLSELAEGAHIDLAPRAGKLSVILDGEDVTEAIRAPEVSHAASVV
                     AVVPGVRHPMVAEQRRAGEKGGVVMEGRDIGSVVFPHADLKIFLDASPEVRAGRRQRE
                     LEEKGEPQEFEKVLEEVHKRDRRDREREMSPLVRAEDAVVVDNTAMDAEETARAIVLL
                     AREKTSAVAG"
     gene            complement(10736..12736)
                     /gene="mutL"
                     /locus_tag="VG272_12110"
     CDS             complement(10736..12736)
                     /gene="mutL"
                     /locus_tag="VG272_12110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HWF14476.1"
                     /translation="MPRIRILAEHVANKIAAGEVVERPASVVKELLENALDAGARSIR
                     VEVESGGKRMIRVIDDGCGMIHDDALLAFERHATSKLRNADDLLSISTLGFRGEALPS
                     IASVSRLLLQTRAEEDAEGTRIEFAGGKLVGVKPEGLPLGTTISVADLFYCVPARRNF
                     LKSETTELGHIASLVTHYALAHPDKQFVLKTPTQEIINTTAVESMADRVYQLFGRQAL
                     DDLFELLPVSSPMRAAITEAELAPEERSAEMVVRGFASRPEVQRGNRNGIYVFVNKRL
                     VRDRLILHAIHEAYRNVLPPSVFPAVLLFLDMPAEEVDVNVHPAKIEVRFRHPQFVHD
                     FTRDSIRQALSRARPIPSFAAPAPNAAGAAEEGTNPSVAAAVASGGQSSSGLPRAVIP
                     PLSGLPASNSPMSRTSIDEIPIDAASATAAALSGDFELTAAPMRPEAQRFRFEGGIGA
                     LTSEPFSDNVPNFSESETLASAGSLTELKPLGQVNSSFIVAVNGEGLWIIDQHVAHER
                     VLFEQHLRARREGRLTGQRMLVPIMVDLAPRQLVIFERIAEELTANGFEVAQMGPRSV
                     AIHAAPAGIATSDAERLLMEILDGTERENAAVSMDSLQAKIAATTSCHAAIKVNMPLD
                     QQKMEWLLAELAKTEAPMSCPHGRPIVLRYSVREIERAFKRI"
     gene            complement(12904..13377)
                     /locus_tag="VG272_12115"
     CDS             complement(12904..13377)
                     /locus_tag="VG272_12115"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWF14477.1"
                     /translation="MSKLPHRTGPGYTYFISSATWERRELFRVPRVAEILVKRIVACR
                     DKGAYQLHEFVVMLNHFHLLITPGESTSLEKAVQMVKGGSSHEIHKQSGNHIEIWQVG
                     FHDWTIRDREDYLTKVGYIHMNPVRAGLVEKPEDWLFGSAFGKFVLDPAPERFRD"
     gene            complement(13466..>14621)
                     /gene="aspS"
                     /locus_tag="VG272_12120"
     CDS             complement(13466..>14621)
                     /gene="aspS"
                     /locus_tag="VG272_12120"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HWF14478.1"
                     /translation="GMDRYFQIVKCFRDEDLRADRQPEFTQLDLEISFPTQDTVFGVI
                     ENVMVRACAVAGITVKGPFRRMEYKDAIRRYGSDKPDLRFGMELNDVTEHFAAARETL
                     RIEGTVQAMVASGAATFSRKQLDELTEKAKSLGARGVYTIKVTAEGATSPLEKNLGAE
                     TLKKIAADVSAKPGDLIVAVTAKEQIPGTDAAAMIAGQLRLLLADQLKLIPKDSWEFV
                     WITGFALFEWNTTEKAVVSAQHPFTGIVDEDVHKLEDPSDEVRLSMRSKGYDLVLNGV
                     ELGSGSIRIHQQDLQDRVFKMLGLSEEQRRNRFGFFLDALTYGTPPHGGIALGLDRVI
                     LLLAGEKSIREVIAFPKTAKAQDLMAEAPSDVDADQLDLLGIQLKPEEEK"
BASE COUNT         3384 a         4116 c         3975 g         3146 t
ORIGIN      
        1 ggcttcatcc cttctcccca aaataaaatt gcaagatttc ggcgctcacc ctgccgaaac
       61 cttgcaatct ctccacacaa aaatcaacaa caatcgcatg taaaaatgcg attttattca
      121 atttttaacg ggggactaaa taatcttgtc cgggagattg gtgtgaaaaa aatactgcta
      181 cgcctatgga aagagcaagt cgctcaggac ttggtcgagt atgccctttt gctcgtcctt
      241 gtttccctcg ttctagtagc ttctctgaga acactgggag ttggagtaac aaaagtttat
      301 tcaggggcag caaccaatct cgcagtcact ggtggcggtg gaagcggtgg ggacggcggc
      361 gatggtggcg gaggagacgg tggcggcgat ggcggtgggg gccgcggcgg ccgataaact
      421 cctcctgcaa gaggaccgat ttggcgagga aatttgccgc attgttaagc gagttgcttg
      481 ccgaggtttc cgcggaaaag ccgaaactgc ggaaacctaa gtattgttgg aaaaagtgag
      541 cgtttttgtc gccgatactt acagttcttt tgcgacgtga atccctattt tctcccggcc
      601 cttttcctca tcccattact tgagggagct tattgattga atgcgggttc tctctcatcg
      661 gagcgtaggc gtatcgggaa gacggcgtgc aaccttcgaa ataagttcgc gctcgattcg
      721 cggcctccgg cgaagaccgt cgccaaaatt tccggaagca cactgtgcga gaccgctttg
      781 gcaaccgccg gacgagcctt acccgcggca catccacaga tcgagttcac tacgaccagg
      841 atggtgcgct tctctcgctt gaacgcagca tccacttctt cagacataca tccgaccaat
      901 ttatcttcca ggaaattttg gcaaggcctc gatttgcttg agcaagcggt tgcccttctt
      961 ttcgggcaag ccgctttaac gcttgtgcca aatgaatcga cgcttcctac caagctatgg
     1021 gtagtgcatc aatttgaatt ttgcagtgct gcggcaaaaa aggctgcccg cccgaaaagg
     1081 cgggcagcct ctacaaaaac aactgctatg cgctgcggtt ttgtagcggc ctctccgtcc
     1141 cgtgatcttc gggacggaga gggcgacttt gctcgcaaat tgaggcacta ccaagctatg
     1201 ctgtgagttg acgttagtca gggattcacc tatactttcg tttcgttgtg ccccgctcgc
     1261 agtactttcc cgtttccaat tttccggacc gagaagcggc ttagagtaga cgcacgattc
     1321 gtagcgaagt tcctgacata ttttctatca tctacattga aattcattca tttgaaggcg
     1381 tgactatgag cgaagcaacc tgccgtcgcg aattggatct tgaaattccc gcggaaaccg
     1441 tacaaaaagc cgtgaagcgc gtgtcaaaag agtttgcgcg tgtcgcgcgc gttcccggat
     1501 ttcgtccggg aaaggcgcca atcaccctga tccagcgccg ttttgccgat gatatcaaga
     1561 gtgaagtgct gcaatcgctt gtgcctgaac aggtcgagcg cgccgtcacc gaaagcaagc
     1621 ttgttccggt cacgcagccg caaatcgaca aggtcgattt ctcggaaaat gggccggtca
     1681 agtttcgcgc cgtatttgaa gtccttcccg aattcgagtt gggacagtat aaggacctgg
     1741 aagtcgaggt cgaagattta aaagtggaag atgcggacgt cgataagggt cttgaggaat
     1801 tgcgagaccg cgccgccacg tttgttccgg tagaaggccg cgcgattgcc gatggcgatt
     1861 acgcgcagct caagctgaac ggcattccga tgggcggcgg cgaaccactt gaagcggaga
     1921 gcgttctttg ccacgtaggc ggcgaagaga caatggagcc cttcaatcag aatcttcgcg
     1981 gagcgagcac gagcgacaaa aagagttttg acgtaactta tcccgccgac tatcccgatg
     2041 ccaagctgca gggaaaaaca tatacgtacg ccgtggaagt tctaggcatc aaagaaaaga
     2101 agcggcccga gttgaccgat gagttcgcga aggatgtgag cgacgcgcaa actctggacg
     2161 agttgcgcaa gaagatgcga gataatctgg aagcggcgcg cacgcacagg cagaacgaac
     2221 aacatcgcga aaagctgctg gcgcaaatcg tgaagggtca tgacttccct gttcccgaag
     2281 cgcttgtcga acaccagatg gattcacgcc tggaacgcac ggtgcgctcg cttgcatcgc
     2341 aaggtgtgga ccctcgcgcc atgaatgtgg attgggtcgc actgcgcaac cggcaaaaag
     2401 accgctcgat cgaagatgtg aaggcggaat tgctgctgga ccgcattgca acggcggaaa
     2461 aaatcgaagt gtccgatgaa gaagtggata aagaaattgc ggcgctcgcc gaacgtagtg
     2521 gcgaatccgc aacagcggtt cgcgccaact tgacaaggca aggagcgctg gataggatga
     2581 aatcgaagat caggagtgag aaaactcttg attggctctg cagcaattcc cggacccgga
     2641 caataaccaa ggagaattga ggcgattccg gccgggttcc ctcgctcaac taaaaaacca
     2701 atgaacctta gaaccgatcg agacgatatg actccgtacg caacaacgtt ggtccccatg
     2761 gtcgtcgaac aaacaaaccg gggggagcga gcctacgata tttattcccg gctcctgaaa
     2821 gataatatca ttttcatcgg gacgcccatt gacgaccatg tcgccaatct ggtgaccgct
     2881 cagcttttat ttctcgaagc ggaagacccc gaaaaagata tttcgcttta cattaactcg
     2941 cccggcggat cgatcaccgc gggcatggcg atttatgaca cgatgcaatt cgtccggccg
     3001 agcgtagtca cgatatgcgt gggacaagcc gcgtccatcg cggcattgct cctgacggcg
     3061 ggagcgccga agaagcgctt ttccctgccc aactcacgaa ttttgattca ccagccgtgg
     3121 atgtccggtc tttccggaca ggccacggat atcgatattc acgcgaagga aatcctgcgc
     3181 atgcgcacgg tcatcaatgg cttgctcgcg cagcacagtg gacatcctct cgacacactg
     3241 gaaaaagacg tcgagcgcga tttcatcatg tccgcggcgc aagccaagga atacggactg
     3301 atcgacgaaa ttattaccaa gcaccgctaa gcgttccaat cagttagtaa tcaaaaaacg
     3361 cggcggtgta cggaaccggc aaaggggcct agaataatcc gcgcgggcag aataattcct
     3421 ttggccggcg gagggcgaca caccgttgaa aaaactcagc actgacgagc cactacgctg
     3481 ttccttctgc cataaaacgc aggagcaagt cgagaaactg atcagctcgc cgtcggacta
     3541 cccgcgcgcc tacatctgca acgagtgcgt gaccgtctgc gaacaaatcc tcgaggaaga
     3601 aaagcgcgaa caggtcaatc ctgttaataa gcgcctgccg cgccctccgg agatcaaagc
     3661 gtttctcgat ggctacgtca tcggccagga taagaccaag aagaagcttg ccgtcgccgt
     3721 ttataaccac tacaagcgca ttttcctgaa ccgccagcca ggcgacgtcg aactgaccaa
     3781 gtcgaacatt cttctgatcg ggccgaccgg aacgggcaag acactgctgg cgcagactct
     3841 ttcgcgcatg ctggatgttc cctttgctat cgtcgacgcc acaactctga ccgaagcggg
     3901 ctatgtgggc gaggacgtcg agaacatcat tctaaagctc ttgcaggcag cagatggaga
     3961 tgtgaccaag gcacagcaag ggattatcta catcgacgag attgacaaga tttcgaagaa
     4021 ggacgaaaac ccatccatca cccgcgatgt ttcgggcgaa ggcgtgcagc aggccttgct
     4081 gaagatcctg gaaggaacga ttgccaacgt tccgccgcaa ggcggacgca agcatccgca
     4141 ccaggaattt accccggtgg acacgacgaa catcctattt atatgcggtg gcgctttcgt
     4201 cgggctggaa aagacaatag agcgccggat tgggcaaaag tcgctcgggt ttcataccga
     4261 tgctcagccc accaccgcgc aacgccgaaa cattgaattg ctcagccaag ctcaaccgac
     4321 ggacctgatc cgctacggcc tgatcccaga attcgttggc cgcttgccgg tcgtcggagt
     4381 tctgggcgac ctggacaagg ccgctttgat gcaaatcttg acggaaccga aaaacgcgct
     4441 catccgtcaa taccagagac tcttcgaatt cgagaatgtt cgcctccggt tttccgacga
     4501 tgcgctcgaa accgtggcgg aacttgcctt gcagcgcaaa gtgggcgccc gcggcctgcg
     4561 catgattctc gaagacctca tgctcgaact catgtaccac ctgccagccc agcgaaaact
     4621 ccgggaattt gtggtgaccg gcgaaatggt ccgtagccgg gagattaact ggtcgctgct
     4681 cgaaaaagcg ggatgatgaa ggagcaggag aaaattacgg aacctatgtt taataaagat
     4741 aagtcggact caaaacgcgt ccccatgatg ccggtgcgcg acctggttat cttcccccaa
     4801 atgatgaacc cgttcatcgt agggcgcgaa acttcgctgc gcgccctgga agaggcgctg
     4861 gccacggaca agaaaatttt tctcgccacg cagcacgatg cttccgtcga cgatcccaaa
     4921 cctgatgaaa ttttttcggt gggcacgctg gccaacattg tgcaaagcgt gcgcctgcca
     4981 gatggcaacc tccgcgtgct ggtggagggc gtcgagcggg ccaaggccct gcagatttcc
     5041 accgacgaag gtttcttccg cgccaccctg aaagtaattc ccaacaaggc cgagccttct
     5101 ccacagatcg aacaagccgt cacgaaagta accgggctgt ttgagcagta tgtaaaactc
     5161 tcgcagagcc tgaattacga cacgatgatt gccgcgaccc gcgtggacga tcccgcgaaa
     5221 ctgtccgaca ccatcgcgtc caacctgcaa attccggtcg aggaaaaaca ggaactcctg
     5281 gaaaacttcg atccgctgga ccgcttgacg cgcgtcggcg aaattctgga aagcgaaatc
     5341 gagaagctca acgtcgatcg caacataaac acccgcgtga agcgccagat ggagcgcgcc
     5401 cagaaagagt attacctcaa cgaaaagctg aaggccatcc acaaggaact gggccgcggc
     5461 gagaagaacg aactcgacga actgaaaaag aagattgaaa cctcgggcat gccgaaggac
     5521 gcgatggaaa aggccaccca ggagctgaaa cgcctggaaa tgatgccgcc aatgtccgcg
     5581 gaatccacgg tgagccgcaa ctatctggac tggctgctcg ctgtaccctg gaaaaagaga
     5641 tccaaggaaa ttcgcgatat cgcgcttgcc gaaaaaattc tggaagaaga gcattacggg
     5701 ctggataagg tcaaggaacg catcctggag ttcctcgccg tccgccagct ggtgaaaaat
     5761 cctcgcggtt cgattctctg ctttgtcggg cctccgggcg ttgggaaaac ttctcttgct
     5821 acatccattg ggcgcgctac gggccgcaaa tttattcgag tttcgctggg cggcgttcgc
     5881 gacgaagcgg aaatccgcgg tcaccggcgc acctacatcg gcgccctgcc cggccagatc
     5941 atccagatga tgaaaaaggc gggaaccgta aatccgattt ttgtgctcga cgaagtcgac
     6001 aagatgtcca ccgacttccg cggcgatcca tccgcagctc tgatggaagt tttggatccc
     6061 gaactgaaca acgcctttac cgatcattat ctcgacgtcg aatatgatct ctccaaggtc
     6121 atgttcgtct gcaccgcaaa cgtgctgcac acgattccac agcccctgca ggaccgcatg
     6181 gaaatcctgc gcatccctgg ctacacggaa gcggaaaaag cgcagatcgc caagcgcttc
     6241 cttgttcgca aggcgcgcga aacgaccggt ctccaggaat cgaacctaac cttcacagat
     6301 gaagcgatta cacacatcat ccggcacttc acgtacgaag ccggcgtccg cagcttggag
     6361 cgcgaaattt ccagcatctg ccgcaaagta gcgcgacgcg tggtgaagga cggcaagcag
     6421 tattcgatca acatcactcc agaaatcgtt ggcgagtttc tgggcgtcat caagtatcgg
     6481 gaagtctggg ccgaaaagaa gaacgaaatc ggcctcacca ccggcctagc atggacggaa
     6541 gtgggcgggt cagtcctttc gaccgaagcg acgatcatgg aaggtaaagg ccgattgacg
     6601 ctgaccggga aactgggcga cgtgatgcag gaatcggcac aggcagccat gagctatgtg
     6661 cgttcgcgca ccgcaacctt tggcctgccg cgcgactttt accgccatat tgacattcac
     6721 atgcacgttc cagaaggcgc catccccaag gatggtccgt cggccggaat cacgattgcc
     6781 acatccatcg tcagcgctct gacgcgtatt ccggtgcgct gcgacgtggc catgacgggc
     6841 gaaatcaccc tccgcggcaa agtgctgcca atcggcggca tgaaagaaaa gttgctggcg
     6901 gcgcaccgtg tgggaattcg aacggtgatc ctcccgaaag ataacgaaaa agatttggcg
     6961 gacattccgg ctgaaatcca agcggatttg accttacggt tcgtcgaaaa tgtggaccag
     7021 gtcctggaaa tcgccctgga gcggcctctg acgcccgcta cgcacgatgc cggccctgaa
     7081 gtggcagcaa agtttggaac cgataccgaa agcgatcaag aattaaccaa ttgagggtga
     7141 cctcagctct tccattccga tgaagcgtgc aagtgcggtt acgcgtagtg catgggggat
     7201 ggagcgtaac ttttaattcg tatcgccttt gatttgaata cccagttttg ctaacccggt
     7261 tggaaatcga atagcacctg tgcggtatcc gccgataaag cataatccgg cctgaactta
     7321 gggcttatct ctaaagagac ttaaggaaat gaaaacgatg agcattagtc ttgcggaact
     7381 gaaagaaaag aacattacag atctggctaa aattgccaag gacctggcga tccccggctc
     7441 cagcggcatg cgcaaacagg agttgatctt ccagatcctc cgcgcccaga ccgaaaagaa
     7501 cggcctgata ttctcggaag gcgtactcga atgcttgccg gatggctttg gatttttgcg
     7561 cgccccggaa tataactacc tgcccgggcc ggacgatatt tacgtatcgc catcgcaaat
     7621 ccgcaagttc gatctgcgca ccggcgacac agtctccgga caagttcgtc ctccgaagga
     7681 cggcgaacgc tatttcgcgc taatcaaggt cgaagcggtt aatttcgagg acccagaggt
     7741 cgcgcgaaat aaaattttct ttgacaacct gacgccgctc tatcctcagg gccgcctgaa
     7801 aatggaaacg acacgcgaaa acctgacggg acgcgttctc gacctgctct gcccggttgg
     7861 aaaaggccag cgcggactga tcgtcgcgcc gccacgcact ggaaagacca tgatgttgca
     7921 ggcgttcgcg aattcggtga ctgcgaatca tccggaagtc gcgctcatcg ttcttctgat
     7981 cgacgaacgg ccggaagaag tcacggacat gcagcgtacg gtgaagggtg aggtcatcag
     8041 ctcaacattc gatgaaccgc cgcagcgcca cattcaagtc gccgagatgg ttttggagaa
     8101 agccaagcgc ctggtcgaac acaagcgcga cgttgtgatc ctgctggact cggtcacgcg
     8161 tctggcgcgt gcttacaatg cggttacgcc gccctccggt aaagttctct caggcggtat
     8221 tgacgccaac gccttacagc gcccgcggcg gttctttgca gccgcgcgaa atgtggaaga
     8281 aggcggatcg ctcacaatta tcgccacagc cttgatcgac acgggcagcc gcatggacga
     8341 cgtgatcttc gaggagttca aaggcacggg caacatggaa atcaacctcg atcgccgcct
     8401 ggtggataag cgcattttcc cagcgatcga catcaaccgt tccggcacac gaaaagaaga
     8461 attgctgatg ccgccagacg aactgaatcg cgtttgggta ttgcgaaagg ttttgaatcc
     8521 cctctctccc gtggaagcaa tggagttgct cttgggacgc ctctccaagt cgaagtcgaa
     8581 cgccgagttc ctggcttcca tgtccagccc cacttaacgg gttcggcgcc tttatttctt
     8641 tcccccttcc gcttcggcca gcgccggagc tttcgaatat ttttgtggat gaccaaggtt
     8701 tcaaatgaaa tatctggctc gacaacctat tctcaatcgc gaacgtgagt tgttcggcta
     8761 cgaactgctt ttccgcaatg gcattcagaa ttcctgcgag gggctggatc tcgaaatggc
     8821 ctccacgtcg gtgctggaca cttcttttct gattggcctg gagaaaattt ccgcagggca
     8881 tccgatgttt tttaactgcc cacgcgagtt cctcatccgc gattacgttt cacttttccc
     8941 gccgaaatcc atcgtggtgg aaattctgga aacgattcat cccgacgaag aaattattga
     9001 cgcctgcaag cgcctgaaag aacgaggcta taaaatcgcg ctggacgatt ttgtcgattc
     9061 cgccaagtgg gcgccgctcg ttgcgctcgc ggatatcatc aaggttgatt tccggctgac
     9121 cggcataacc gaacaacgcg agattgtttc ccgctatgcc agcaaaaaaa ttaagatgct
     9181 ggcggaaaaa gtggaaacgc agcaagaatt tgccgcaggc atgcagatgg gatactcgct
     9241 gtttcagggc tattttttct gccaccctga gatgatgtcg caccgcggat tgccgtcctt
     9301 caagcttacg tatgttgaac ttttgcgggc ggcgacggcc cctgaattta atattcagga
     9361 actggccgtg aaaatcaaat acgagccttc gctcactttc cggttattgc gctatctgaa
     9421 ttccgcgttt ttcggcctgc ggggagaaat tcactccgta caacatgcgc tttcgctgct
     9481 gggagaacgc gaattgcgca aatggatcgc cgtagtatcc gtcggcgtgc tggcggaggg
     9541 caagcctgac gaactgatga ccgtgccgct catccgctcg agattctgcg aattactggc
     9601 tcggctcgct catattccgg gagatgcgaa tgacttgttc ctgatggggc ttctttccct
     9661 gatggacgcg attctcgatc agccaatcga atccattttg aaggaaatgc cggtacgggg
     9721 cgaaattaaa gatgcgctgc ttcatcagaa gggtctgtac tggcaactgc tggaaattac
     9781 gattgcgcac gaacaagcgg actgggcaaa agtcagccgg ctcgtatcgg ccatcgggat
     9841 gaatgatgag caagtttccg cgttgtatat ttccgccgtg gattggtgca cggcgctgcg
     9901 ccaaagtatt tttgttccgg ctacccgctg aagattttct gccgacccgg ttacgaataa
     9961 tcggttttct acggggatta tagaaattct cgatggaagc ttgcacttgc ttctcgcggc
    10021 tcgcggaatt tgttcgctgc ttcaccctgc cacagcgctc gtcttttctc tcgccagcaa
    10081 cacaatcgcc cgcgccgttt cttccgcgtc catcgccgtg ttatctacca ccacagcatc
    10141 ttcggcgcgc acgagcggag acatttcgcg ctcgcggtcg cgccggtcgc gcttatgcac
    10201 ttcctccaaa actttctcga attcctgtgg ctcgcccttt tcctcaagtt cgcgctgccg
    10261 gcgtcccgcg cgcacttcag gtgaagcatc cagaaaaatt ttcaaatccg cgtgcgggaa
    10321 aacgacggag ccgatatcgc gcccttccat caccacgccg cctttttcgc ccgcgcgccg
    10381 ctgttccgct accatcggat ggcggactcc cggcacaacg gcaaccacgg acgcggcgtg
    10441 tgaaacttcc ggagcacgaa tcgcttccgt cacgtcctcg ccatccagga tgacgctcaa
    10501 cttccctgcc cgcggcgcaa ggtcaatatg agcgccctcg gcgagctcag acaggcgctc
    10561 cggcgaatcg agcggcacgc cgccacgcaa agctttcagg cccacagcgc ggtacattgc
    10621 gccgctgtcc agatgcgcgt aacccagcag cgccgccacg cgccgcgcca ccgtgctttt
    10681 gcccgagcct accgggccgt caatcgcgat gatgagtctg tcgttcatga tgtcatcaaa
    10741 tccttttgaa cgcgcgctcg atttcgcgaa ccgaatagcg cagcacaatt ggtcgcccgt
    10801 gagggcagct catcggcgct tccgtttttg cgagttccgc gagcagccat tccatttttt
    10861 gctggtcgag cggcatgttc actttaatcg ccgcgtggca agaagtggtg gccgcaattt
    10921 tcgcctgcag cgagtccatc gaaaccgccg cgttctcccg ctccgtgccg tccagaattt
    10981 ccatcagcaa acgctctgcg tccgaggtgg cgattccggc gggcgcagcg tgaatcgcaa
    11041 cgctgcgcgg gcccatttgc gctacttcga aaccgttcgc ggtgagttcc tcggcgatac
    11101 gttcgaaaat cacgagctgg cgcggggcaa ggtccaccat gatcggtacg agcatgcgct
    11161 ggccggtcag ccggccttcg cgtcgtgcgc gcaggtgttg ttcgaacaag acacgctcat
    11221 gcgccacgtg ctggtcgatg atccacagac cttcgccgtt cacggccaca atgaaacttg
    11281 aattcacctg accgagcggc ttcagttccg tgaggctgcc cgcactggcc agagtttccg
    11341 attcgctgaa gttcggcacg ttgtcggaaa atggctcgct ggtaagcgct ccaatgccac
    11401 cttcgaatct gaagcgttgc gcctcaggac gcatgggagc ggcagtcaat tcgaaatcgc
    11461 cggacaaggc agcggccgtt gcactcgccg cgtcgatggg gatctcgtcg atggatgtcc
    11521 ggctcatggg ggaattgctt gcagggagcc cgctcaaagg tggaatcacc gcgcgaggca
    11581 atccagagct cgattggcct ccgctggcaa ccgccgcagc gacggatgga tttgtgcctt
    11641 cttccgccgc gccggctgcg ttcggtgcgg gtgctgcaaa actcggaatg ggccgcgcgc
    11701 gtgaaagcgc ctggcgaatc gaatcgcgcg tgaagtcatg cacgaactga ggatggcgga
    11761 agcgcacttc gatttttgcc gggtgaacgt tcacatccac ttcttcggca ggcatatcga
    11821 gaaacagcag cacggccgga aaaacggaag gcggcagaac gttgcggtac gcttcgtgaa
    11881 tggcgtgcaa aatcaggcgg tcgcgcacga ggcgcttgtt cacgaaaaca taaattccat
    11941 ttcgatttcc gcgctgcacc tccgggcggg acgcgaagcc gcgcacaacc atttccgcgc
    12001 tgcgctcttc gggcgcgagt tccgcttccg tgatggccgc gcgcatgggc gaagaaaccg
    12061 gcagcaattc aaacagatca tcgagcgctt ggcgtccaaa aagctggtag acgcggtccg
    12121 ccatcgattc cacggccgtc gtattgatga tttcctgcgt cggcgttttt aatacgaatt
    12181 gcttgtcggg gtgcgcaagc gcgtaatgcg tgacgagcga ggcgatgtgc cctagctccg
    12241 tggtttcaga ctttagaaaa ttgcgtcgcg cgggaacaca ataaaaaaga tcggcgacgc
    12301 tgatcgtcgt cccaagcggc aaaccttccg gctttacgcc gaccagtttt cctcccgcga
    12361 actcgatgcg cgtgccctcc gcatcttcct cggcgcgcgt ttgcagcaac aggcgggaaa
    12421 ccgacgcgat ggaaggcagc gcttcgccgc gaaatccgag cgtggagatc gacagcaagt
    12481 catccgcatt gcgcagcttc gaggtcgcgt gccgctcaaa cgccagtagc gcatcgtcat
    12541 gaatcatgcc gcagccgtcg tcaatcacgc gaatcatgcg cttgccgccc gattcgactt
    12601 ccacgcgaat cgaacgcgcc cccgcatcca gcgcgttttc gagcagctcc ttcacaaccg
    12661 aagcgggccg ctcgaccact tcgccagcgg cgatcttgtt cgcgacgtgc tccgcaagta
    12721 tgcgtattcg tggcatttat tttcgcgcca tttcttctcg tgccaattct tcccacatca
    12781 attcttttcg tgatatttct ttttgttgca ttgtttcttt tcgttgcgat gtttctttag
    12841 gagggccggg cttcagcccg gccgtaatcg atctcttcca ttaagggctt tagcccctga
    12901 agttcaatct ctgaatctct ccggtgccgg gtccagaaca aatttcccaa atgctgaacc
    12961 aaacagccaa tcttctggct tctcaaccaa acctgcacga accggattca tgtgaatata
    13021 cccgactttc gtcaggtagt cttccctgtc tcgaatcgtc caatcatgaa aaccgacttg
    13081 ccagatttct atatggttcc cactctgttt atgaatttcg tgcgaacttc caccttttac
    13141 catttgaacg gccttctcta aagaagtgga ttcgccggga gttattaaaa ggtggaaatg
    13201 attcagcatg actacaaatt catgcagctg ataagcacct ttgtcacggc acgcgactat
    13261 gcgcttgacc aatatttccg ccactctcgg tacacgaaat agttctcgcc gttcccatgt
    13321 tgcggacgaa ataaaatacg tgtagccggg gccagtcctg tgaggcagtt tgctcatcgg
    13381 aacttcaggg gctgaagcct cgttttctct cgactcttac gtcggggctt aagccccgac
    13441 ctccgaaacc gtcacccgag aaaaactatt tttcttcctc gggcttcagt tgaataccca
    13501 gtaagtcgag ttgatccgcg tctacatccg acggggcttc ggccattaaa tcctgcgcct
    13561 tcgccgtctt ggggaaagcg atcacttcgc gaatcgattt ttcaccagcg agcaatagga
    13621 ttacgcggtc gaggcccagc gcgattccgc cgtgcggagg tgttccgtac gtcagagcat
    13681 cgaggaaaaa gccaaagcgg ttgcggcgct gctcttcgct caggccgagc attttgaaga
    13741 cgcgatcctg caaatcctgc tggtggattc ggatactacc cgagccaagc tcgacgccgt
    13801 tcagcaccag gtcgtagccc tttgagcgca tggacaggcg cacttcatcc gaagggtctt
    13861 cgagtttatg aacatcctcg tccacaattc cggtgaaagg atgttgcgcg ctgaccaccg
    13921 ctttttccgt cgtgttccat tcaaagagcg cgaaaccggt gatccagaca aattcccagc
    13981 tgtctttggg aattagtttt aattggtccg cgagcaacaa gcgcaattgt ccggcgatca
    14041 tcgcggctgc atccgtgcca ggaatttgtt ccttcgcggt gacggcgacg atcaggtctc
    14101 cgggcttggc ggacacatca gcggcaattt tcttcagcgt ttccgcgcca agattttttt
    14161 caaggggcga ggtcgcgcct tcggccgtga ctttaatcgt gtacacgccg cgcgcaccca
    14221 gcgattttgc tttttcggtg agttcgtcga gctgcttgcg cgaaaacgtg gctgcacccg
    14281 acgcaaccat cgcttgcacg gtgccttcga tgcgcaacgt ttcgcgcgca gccgcaaaat
    14341 gttcggtcac atcgttcagt tccatcccaa agcgcaggtc aggcttgtcc gaaccgtacc
    14401 gacggatggc gtccttgtat tccatacgcc ggaacgggcc ttttacggtg attcccgcaa
    14461 ccgcgcaggc gcgcaccatc acgttttcaa tcacgccaaa aaccgtgtcc tgcgtgggaa
    14521 aggaaatctc caggtcaagc tgcgtgaatt ccggctggcg gtcggcccgc aaatcttcat
    14581 cgcggaaaca tttgacgatt tggaagtagc ggtccattcc g
//
LOCUS       DASZMB010000258         4019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1895529, whole genome shotgun sequence.
ACCESSION   DASZMB010000258 DASZMB010000000
VERSION     DASZMB010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1895529"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..818
                     /locus_tag="VG272_12125"
     CDS             297..818
                     /locus_tag="VG272_12125"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HWF14479.1"
                     /translation="MIKKLLWFAFCSLPIVTFAAMRPEPTVPGSSLSTPEDAVGPLKV
                     IIYKSRRGGTSPGIFSAVARAQKPQGGLTVAAQLQSYIQVQALKHGVSPKLALWIVKH
                     ESRFNPDARGDSDASRGLWQISKIYHPEVSDAVAFNARSSTQWSLERIREGKANEWST
                     YRFCRTLYEDCPF"
     gene            complement(937..2340)
                     /locus_tag="VG272_12130"
     CDS             complement(937..2340)
                     /locus_tag="VG272_12130"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWF14480.1"
                     /translation="MIFKQFYLPCLAHASYIIGDEATGTAVVVDPQRDTDQYTAFASA
                     HALKIKYVFLTHLHADFIAGHLELRDREGATICLGARAKAGYAFTPMHDEELLEFGRV
                     RLKVLETPGHTPESISIIVFDLNANEQKPYGVLTGDTLFIGDVGRPDLRAALGWSAAQ
                     LGSMLFDSLHNKLLPLPDETLVYPAHGAGSLCGKSLSKETVSTIGEQRRVNYALQPMT
                     KEAFLAVVTADQPDAPAYFTYDAVMNSEERPTLDQTLAHVTPMTLEDVLALQQIGAQI
                     LDTRDPGEFAAAHLAGSISIGLGGQYATWAGTVLDREHPIVIIADQGREDEATTRLGR
                     IGFDHVVGYLKDGLMSLQTRPDLTVKTERVSAPFAAELLSQLEPPVAIDVRGPVERTQ
                     KYIAGSLNIPLNHLTENVTKIPKGRPLLVYCAGGYRSSIAASLLQRSGFDQVSEIAGG
                     IVSWEAAKLPVRSGDVA"
     gene            complement(2366..3022)
                     /locus_tag="VG272_12135"
     CDS             complement(2366..3022)
                     /locus_tag="VG272_12135"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HWF14481.1"
                     /translation="MLRINDEAPNFTAETSQGTINLYEWMGDGWAILFSHPKDFTPVC
                     TTELGYMAGLEPEFKKRNTKIIGLSVDPVTSHAKWASDIEETQGHKVNFPMIGDPELK
                     VAKLYDMLPAEAGDSCEGRTAVNNQTVRTVFVIGPDKKIKLMLSYPMSTGRNFDEVLR
                     VLDSMQLTARHKVATPVNWKQGDDVIILPAVTEDEAKLKYPGGWKSPRPYLRIVPQPK
                     "
     gene            complement(3259..>4019)
                     /locus_tag="VG272_12140"
     CDS             complement(3259..>4019)
                     /locus_tag="VG272_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14482.1"
                     /translation="YWYNGLQVQVNKAMSHGLEIGGSYTYSKNMDSGGGSVASDPFRN
                     SVSTLLWFCKACRRGLSDQDQRHNLTVHYQWDIPTPSSFGKPLKMVLGDWETGGVLTI
                     ASGTPFTVLLAGDPLGMSTTDPYQYPDKVPGAACTNPVNPQNAVSYIKLQCFTAPAIS
                     TQLGNSGRNNAIGPGLVDLDFSLFKNIPIHEAFKAQFRAEMFNLTNRPNYNSPNDNRA
                     ILNADGSVNGSTPGRISLLNVPSRQIQFALKFTW"
BASE COUNT          898 a         1133 c         1049 g          939 t
ORIGIN      
        1 tgccagacat taattattca acagtatata gtcccggttt tccgcatact ttcttcccga
       61 agtgcatttc ttctgaagcg caaaaaaact caccgtcgcc aatattatct ctaaggtaaa
      121 tcccctaggg ccataaaaaa tcgaaaatcg ggtgtcttgc cgatgccttg acgccgaccc
      181 agggaggaaa ataattgggc gtctttggac cagattgacg aggcggcgat agcgggaagg
      241 atttagtatt cgctttctct tcgcctttgt gtataatctc tgccttgggg gcgtccatga
      301 tcaagaagct gctgtggttc gcattctgct cacttcccat tgtaacgttt gcggcgatgc
      361 gtccggaacc caccgttcct ggttcttccc tgtcaacgcc ggaggacgcg gtcggacccc
      421 ttaaggtgat catctacaaa agccgacgcg gcggcacttc gccaggcata ttttccgcgg
      481 tggccagagc gcaaaagccg cagggcggcc tgaccgtcgc ggcgcaattg cagtcttaca
      541 tccaggtaca ggccctgaaa cacggcgtca gccctaagct cgcgctatgg atcgtcaagc
      601 atgaatcgcg attcaatccc gatgcgaggg gcgatagcga cgccagccgc ggcctctggc
      661 agatttcaaa aatttatcat cccgaagttt ccgatgcggt tgcctttaac gcgcggtcca
      721 gcacgcaatg gagcctggaa cgaattcgtg aaggaaaagc caacgaatgg tcgacgtacc
      781 gtttctgcag aaccttatac gaagattgtc ccttttagtt tacgtggcat aggcttacgt
      841 ggcataggct tcagcctgtg cgggttttgt cctgcaacta ttaaatgtct aagactttga
      901 aagcattggc aagacgaaaa ccgcacaggc tcaagcctat gccacgtctc cagaccgaac
      961 tggcagcttc gcggcctccc aactcacaat tccgccggca atttcactga cttgatcaaa
     1021 cccgctgcgc tgcaacaaac tggccgcaat tgaagaccgg tacccgcccg cacaataaac
     1081 aagcaggggc cgccccttag gaattttcgt cacattttca gttaagtgat tcaacggaat
     1141 gttcaggctg cctgcaatat atttttgcgt gcgctcgacc gggccgcgca catcgatcgc
     1201 gaccggcggt tctaactgtg acaaaagttc cgccgcaaat ggcgcgctga ctcgctccgt
     1261 cttcaccgtc aaatccggtc gcgtttgcaa actcatcaat ccatctttca aatatccgac
     1321 gacgtggtca aacccaattc ttccgagccg agttgtcgct tcatcttcgc ggccctgatc
     1381 tgcgatgatc acgatcggat gctcgcgatc gagcacagtt ccagcccatg tggcatattg
     1441 tccgcccaga ccgatgctaa tgctgcccgc aagatgtgcg gcagcaaatt cgccaggatc
     1501 gcgggtatcg aggatttgcg cgccaatctg ctgcagcgcg agcacgtcct ccagcgtcat
     1561 gggcgtcacg tgcgccaaag tctgatcgag cgtcggccgc tcttcgctgt tcatcacggc
     1621 atcgtatgta aaatatgcag gcgcatcggg ctgatcggcg gtcaccaccg ccagaaatgc
     1681 ttccttcgtc atcggttgca gcgcgtaatt gacgcgccgc tgctcgccaa tcgtggacac
     1741 cgtttccttg ctgagcgatt ttccgcatag cgaccctgcg ccgtgcgccg ggtacacgag
     1801 cgtttcgtcc ggaagcggca gcaatttatt gtgcagcgaa tcgaaaagca tcgaccctag
     1861 ttgagcggcg gaccagccca gcgccgcgcg caaatcgggg cgtccgacat cgccgataaa
     1921 cagagtgtct ccggtgagca cgccgtaggg cttttgctca ttcgcattta ggtcaaaaac
     1981 gataatagaa attgattctg gagtgtggcc gggcgtttcc agaactttca ggcgcacacg
     2041 cccgaattcc agaagttctt catcgtgcat gggtgtaaac gcatagcccg ctttggcccg
     2101 cgcgcccaaa caaatggtcg cgccttcgcg gtcgcgcaat tccaagtgcc ccgcgatgaa
     2161 atcggcgtgt aagtgggtga ggaatacgta tttgattttc agtgcgtgtg cggaggcgaa
     2221 cgcggtgtac tggtccgtgt cgcgctgcgg atcgacaacc acagccgtgc ctgtcgcttc
     2281 gtccccaatt atgtaagacg cgtgcgccag acacggcaga tagaattgtt taaagatcac
     2341 ttgggattgc ccccgtccgc aatatttact tgggttgagg aacgatccgc aaataaggac
     2401 gcggcgactt ccaaccgcca ggatatttca atttggcttc atcttctgtc accgcgggca
     2461 aaatgatgac gtcatcgccc tgcttccagt tcacgggcgt ggctactttg tgccttgccg
     2521 tgagttgcat ggagtcgaga acgcgaagca cttcatcaaa gttgcggcca gtgctcatag
     2581 ggtagctgag catcaacttg atcttcttgt ccggcccgat cacgaacaca gtgcgcacgg
     2641 tttgattgtt caccgccgta cgcccttcgc acgaatcgcc ggcttccgcg ggcagcatgt
     2701 catacagctt ggccaccttc agttccggat cgccgatcat cggaaaattc actttgtgcc
     2761 cttgcgtctc ctcgatgtca gaagcccact tggcatggct tgtaaccgga tcgacgctca
     2821 ggccgataat ttttgtgttg cgcttcttga actccggttc gaggccggcc atgtatccaa
     2881 gttccgtggt gcacaccggc gtaaaatctt tggggtgcga aaacaaaatc gcccagccgt
     2941 cgcccatcca ttcgtaaaga tttattgtcc cttgcgacgt ttccgcagtg aagttcggtg
     3001 cttcatcatt aatacgcagc atgaatttat tctcctcagg gaaaagtgac gcgcgcattc
     3061 tacgcccgcc ccgcccctcg atacaatgaa tttgcggtta caagaccgta cacggaattg
     3121 gatgtgggtt aagtagcata cgcttcagcc tgtggctttt agtccttgtc agaactaaac
     3181 cccacaagtc gaagcccatg cgatgcgcga gagccgtacg gctacgtttc gcagcgaagc
     3241 gacagtcgtc gaaaatccct accaggtaaa tttcagcgca aattgaatct gcctcgaagg
     3301 cacattcaga agcgaaatcc ggccaggcgt tgagccgttg acgcttccat ccgcgttcag
     3361 aatagcccgg ttgtcgttgg gcgaattgta attcggacga ttcgtcaggt tgaacatttc
     3421 agcgcggaac tgcgctttga acgcttcatg gatcggaata ttcttgaata atgagaaatc
     3481 caaatcgacc agtccgggcc caatcgcatt attgcgccct gaattaccga gttgcgtgct
     3541 gatcgccggg gcggtgaagc attgcagctt gatgtaggac acggcattct gcggattcac
     3601 tgggttcgtg caagcggcgc caggaacttt gtccggatat tgatatgggt ccgtcgtgct
     3661 catcccaagc ggatcgcctg caagcaatac ggtgaacgga gtgccggacg caatcgtcag
     3721 cacgccaccc gtttcccaat ctcccaaaac catcttcaga ggttttccaa acgaagacgg
     3781 cgtgggaata tcccattggt agtgcacagt caggttgtgg cgctggtcct gatcggagag
     3841 tccgcgccta caggccttac aaaaccatag cagcgtggaa acagaattgc ggaacgggtc
     3901 cgaagccact gaacctccgc cgctatccat gtttttcgaa tacgtatacg agccgccaat
     3961 ttcgagcccg tggctcatcg ctttattgac ctgaacctgg agcccgttat accagtatt
//
LOCUS       DASZMB010000259         6361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2734981, whole genome shotgun sequence.
ACCESSION   DASZMB010000259 DASZMB010000000
VERSION     DASZMB010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6361
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2734981"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VG272_12145"
     CDS             <1..681
                     /locus_tag="VG272_12145"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWF14483.1"
                     /translation="NFRIEPGERVALVGANGHGKTTIVKLITRLYEVTSGQILLDGID
                     LREYNLEDLWKNIGVIFQDFMRYDMTVADNIATGKIEERGDAFRIRAAAFKSLAETVI
                     HKFPENYNQMLGCRFAGGVDLSGGEWQKIALARAYLRDAQLLILDEPTAALDARAEHE
                     VFQRFADLTKGKMSVLISHRFSTVKMSDRILVLENGRIAEQGAHNQLMRSGGSYAEMF
                     ELQAAHYR"
     gene            814..2337
                     /locus_tag="VG272_12150"
     CDS             814..2337
                     /locus_tag="VG272_12150"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14484.1"
                     /translation="MNPLKRTSAVRFISRILILSLIFLPRIASAQAQTEKIDVNLAGP
                     SHDFPHFWERMFGSGRAVLALRDQFRRDLSVVKDATGFEYIRFHAIFHDEVGLYNEAE
                     DGKVSYNFSYIDQIYDGLLANHVRPFVELSFMPQKLAAEPLLHSFWYKPNISPPKDYA
                     KWDDMISHFATHLIERYGIEEVSQWYFEVWNEPNLDFWGGTPKEDTYYKLYDETARTL
                     KQVDSRLRVGGPSTAQAAWVDRFIKHAVDSKVPVDFVSTHVYANDSSEDVLGTHEKIS
                     REDMVCRAVQKVHEQVQKSAYPNMPLIWSEYNASYKNEPEVTDAEFMAPWLANTIREC
                     DGIVTMMSYWTFSDVFEEQGVVKEPFYGGFGLLAEDGLPKPSFNAFKILHGLGNTRID
                     NESRSALVTKRADGTVVMAVWNLYLPGTAGEAKHFVIDVRGAKEFHRAAISRVDAAHG
                     SLLATYTGMGKPSYPTQAQLQTLRNAASLPPAEQTRLHQGELALDVPAQGLAVIEFH"
     gene            complement(2334..3731)
                     /locus_tag="VG272_12155"
     CDS             complement(2334..3731)
                     /locus_tag="VG272_12155"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HWF14485.1"
                     /translation="MTRRRLLKMFARMAAVTPLAAQTRRGTSSEPTRQNPSTAAPVIQ
                     PKPFSDQDDAFLQELEASNFHYFWDQANPDTGIVRDRCNVRNPDKSDLGSIAATGFGL
                     TALCIGDKRGFISHAEAKERVLNTLRFHWKKLPNHRGFFYHWANINTGERLWDSEISS
                     IDTAILLCGILTCREYFQNAEISGLAYDIYNRVDWDWLSEDTAILPHGWRPESGFLQY
                     RWDNYSEMMMMYLLGLGSDSHPLPAESWDAWKRTPFEYDGIRYIGSFAPLFVHQYSQA
                     WFDFRGKRDKYTDYFHNSVVATDVHRRFCIDLAGEFPDYSDDLWGITASDSVHGYAAW
                     GGPPSVGPIDGTVVPCAAGGSLPFLKEPAMRVLRTMKNRYGNGAWSPYGFVDAFNPLT
                     NWYDHDVIGIDTGITMAMVENARTGFVWDTFMKNPETERGMKRAGFVSDPALELQDKT
                     ALAENFAGTPPRKSV"
     gene            3899..4903
                     /locus_tag="VG272_12160"
     CDS             3899..4903
                     /locus_tag="VG272_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14486.1"
                     /translation="MFHGRRLLLWTIVASVGSLLAIFLSFGLLRYHRRGPIVLRGAVI
                     KQDEDPNKQSPIMDVDISEANGLAANDTKSNFTGFFSIPLEAGVGRNRSVTLRFLHPD
                     YVPLELTELVGDTLTVVHLVPIHREEPAAPVQTDHPPIAVANIRIRYSTQAVTQENIG
                     TGVKTFQVRNVGNIPCENSNPCSPDGKWKASVNSMSMDAGEGNVFENARVPCIAGPCP
                     FTKVDYDGFSQGGRIIKVSVRNWSDTTTFLFQAEVFRPQIYDLVQMTYPIILGTSLNF
                     TLPPTAEGPSIEAELNGENIIFPLGPIPVLSWADCERSVAKNKARLYRCELNSGYQFR
                     "
     gene            4965..>6361
                     /locus_tag="VG272_12165"
     CDS             4965..>6361
                     /locus_tag="VG272_12165"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HWF14487.1"
                     /translation="MQTIGSLAAQAVLLVSMVRTPLTLSELAPPIPHPEIDNRSLQDV
                     LTYIDGAWGTLTRSMEQCQSLEDPKTDSEAIVYLPAGMAVPESLSKLQASCRVRIEHL
                     PNQITKVGEFDASKIPGEGLLYLENPYVVPGGQFNEMYGWDSYFILRGLIREHSLELA
                     KGMVENFFFEIEHYGGILNANRTYYLTRSQPPFLTSMILAIYEAEKAEGKTDLDWLER
                     AYAFAERDHESWNKTPHLAGNTGLSRYYDFGDGPVPEIMGDPSQYYRGAADFFLAHEN
                     FAKLNLVREGAHETQKSAQEPRFPVLTCNAKADEAETGKCAPAYWVGLSSEFYKGDRS
                     LRESGFDVTFRFGPFGAATHHFAAVCLNSLLYKTEMDLVEMSNILGHPERAEHWKQTA
                     ADRQKRMDKYFWDADEGMFFDYNFVVGKRSQYAYATTFYPLWAGLATKEQAKAIVQNL
                     GVFEQHGGLAMSRTD"
BASE COUNT         1520 a         1664 c         1763 g         1414 t
ORIGIN      
        1 aacttccgca tcgaaccggg cgagcgggta gccctcgtag gagcaaacgg gcacggcaaa
       61 acgaccatcg tgaagctcat cacccggctg tacgaagtga cgtcagggca gatcctgctc
      121 gacggtatcg acttgcgcga atacaacctg gaagatctgt ggaagaacat tggagttatt
      181 ttccaggact tcatgcgcta tgacatgacc gtggccgaca acatcgctac gggcaagatt
      241 gaagagcgcg gtgatgcttt ccgcattcgc gctgcggcgt tcaagagctt ggctgagact
      301 gttatccata aattccctga aaactataac cagatgttgg gctgcaggtt cgcgggaggc
      361 gtggacctct ctggcggcga atggcagaag attgctctgg cccgtgcgta cctgcgcgat
      421 gcgcagttgc tgatcctcga cgaaccgaca gccgctctcg acgcgcgcgc cgagcacgaa
      481 gttttccaac gcttcgcgga tctgaccaaa ggcaaaatgt ccgtgctcat ttcccaccgt
      541 ttctctacgg tgaaaatgtc tgaccgaatt ctggttctgg aaaatggcag aatcgctgaa
      601 cagggcgcgc acaaccaact gatgcgcagc ggcggcagct acgcagagat gtttgaattg
      661 caggcggcgc actaccgctg atcgcttgca cccttgtgta atcgggtggg cggtagtatc
      721 aatgaagagg aaatagatgg gagcagggat ggccaatgaa tgcaaggcat ccctgcgaac
      781 atttacagct cccggacgcg gagttcctta cccttgaatc ccctgaagcg aacgagcgcc
      841 gtgcgtttta tttcacgaat cctcatactt tccctcatat ttttgccgcg gatcgcgtct
      901 gcgcaggcgc agaccgaaaa aatcgacgtg aatctcgcgg gtccaagcca cgactttcct
      961 catttttggg aacgcatgtt tggatcgggt cgtgccgtcc tggcgttgcg cgatcaattc
     1021 cggcgagatc tgagtgtagt aaaagacgcg accggctttg agtacatacg atttcacgcg
     1081 attttccatg acgaagttgg gctatacaac gaggccgagg acggaaaagt ttcttacaac
     1141 ttttcgtaca tcgaccagat ttacgacggc ttgctggcca accatgttcg tcccttcgta
     1201 gagttgagct ttatgccgca gaagctggcg gcagaaccgc tgcttcactc gttctggtat
     1261 aagccgaaca tttctcctcc gaaggactac gcaaaatggg acgacatgat ttcccatttc
     1321 gcgacccacc tgatcgagcg ctacggaatc gaggaagtct ctcagtggta ttttgaagtg
     1381 tggaacgaac ctaatctcga tttctggggc ggcacgccga aagaagacac ctactataaa
     1441 ctgtatgatg aaacggcgag aaccttgaaa caggtggaca gccggctgcg cgtcggcggt
     1501 ccatcgacgg cgcaggcagc atgggtggat cgttttatca agcacgcagt cgacagcaaa
     1561 gtccccgtcg attttgtctc gacccacgtc tatgcaaacg attcaagcga ggacgtactc
     1621 ggcacgcacg aaaaaatttc gcgcgaggat atggtgtgcc gggccgtaca aaaggtccac
     1681 gaacaggttc agaagtccgc ctatccaaat atgccgctca tctggagcga atataacgcc
     1741 agctacaaaa acgagcctga agtgacggac gccgaattca tggcgccatg gctggcgaat
     1801 acgattcgcg aatgcgacgg aattgtcacc atgatgtctt actggacttt ttccgatgtg
     1861 ttcgaggaac aaggtgtggt gaaagaaccg ttttacggcg gatttggatt gctcgcggaa
     1921 gacggcctgc caaaaccttc gtttaatgca ttcaaaatat tgcatggact tggaaatacg
     1981 cgaatcgaca acgagtcccg gtccgctctc gtgaccaagc gcgccgacgg gaccgtcgtc
     2041 atggctgtct ggaatttata tttgccggga actgctggag aagcgaagca tttcgtaatt
     2101 gacgtccgtg gcgcaaagga attccaccga gccgcaattt cgcgcgtgga tgccgctcac
     2161 ggttccctac tcgccacgta cacgggtatg ggcaagccca gctatcccac gcaagcccaa
     2221 ctgcaaactc tgaggaatgc cgcaagcctt ccgcccgcgg agcaaacgcg actgcatcaa
     2281 ggcgaactcg cgctggacgt gcctgcacaa ggcctggcgg tgattgagtt tcactaaacg
     2341 gattttctag gcggtgtgcc ggcgaaattt tcggcgagcg ccgttttatc ctgtaactcc
     2401 agcgcagggt cggatacaaa tccggctctc ttcatgcccc gctcagtttc ggggtttttc
     2461 atgaaagtat cccacacgaa tcccgtgcgc gcattttcga ccatcgccat tgtgataccc
     2521 gtatcgattc cgattacgtc gtggtcgtac caattcgtca gagggttgaa cgcgtcgaca
     2581 aacccgtacg gggaccaggc gccgttgccg taccggtttt tcatcgttcg aagaacgcgc
     2641 atcgcaggct cttttaaaaa cgggagtgag cctccggccg cgcacggcac gacggtgcca
     2701 tcaatcggtc cgacggatgg cggcccgccc cacgcggcgt agccatgcac ggagtccgag
     2761 gcggtgatgc cccacaaatc gtcgctgtaa tcgggaaatt cgccggcgag gtcaatgcag
     2821 aatcggcgat gcacatcggt agccacaacg gaattgtgaa aatagtctgt gtacttgtcg
     2881 cgcttgccgc gaaagtcgaa ccaggcttgc gaatattgat gcacgaacaa gggcgcgaag
     2941 gaaccgatgt agcgaatgcc gtcgtattcg aacggcgttc gcttccaggc gtcccagctt
     3001 tcagcgggca gcggatggga gtcggaaccg agtccgagca agtacatcat catcatctcg
     3061 ctgtaattgt cccagcgata ttgcaggaat ccgctttccg gtcgccatcc gtgtggcagg
     3121 atggcggtgt cttcggacag ccagtcccaa tcgacgcgat tgtaaatgtc gtaagcgagg
     3181 ccgctgatct ccgcgttctg gaaatattcg cggcaagtta gaattccaca caggagtatt
     3241 gcggtatcga tggaagagat ttcggaatcc cacagtctct cgccggtgtt aatattcgcc
     3301 cagtggtaaa agaagccgcg gtgattggga agtttcttcc aatgaaaacg caaagtattc
     3361 agcactcgct ctttcgcttc ggcgtgagaa ataaagccgc gcttgtcgcc gatgcacagc
     3421 gcggtcaatc cgaatccggt ggcggcaatg cttcccaaat cgcttttgtc ggggttacga
     3481 acgttgcagc ggtcgcgaac aatacccgtg tccggattgg cctgatccca gaaatagtga
     3541 aagttggagg cctccaactc ctgcagaaac gcgtcatcct gatcggagaa tggtttcggc
     3601 tgaatgacgg gcgcggccgt cgagggattc tgccgcgtgg gttctgacga cgtaccacgg
     3661 cgagtttgcg ccgcgagggg cgtaacggcg gccatgcgcg caaacatttt caacagtctc
     3721 cggcgagtca aaccagcttt ttctcgattg ccgtcgccga tccacataca attgattata
     3781 gacgttcttt aattcaataa aagagcgctg attggctaag atgaaagaat gttatttcag
     3841 gggagcggat ggcgcaccat catgctaact tgtagcttcc agctcaaaag gtgacacgat
     3901 gttccatgga cgtagattac ttctctggac aatcgtcgcg agcgttggaa gtctgctggc
     3961 tattttttta agtttcggtt tgcttcgcta ccaccgccgg ggtccgatcg tgctgagggg
     4021 tgcggtcatc aagcaggatg aggatcccaa taaacaatcg ccgatcatgg atgtagatat
     4081 cagcgaagcg aacggcctcg ccgcgaacga tacaaaatcc aacttcaccg gattcttttc
     4141 cattccgctg gaagcgggcg tgggtcgcaa tcgctccgtg acgctccgct ttttgcatcc
     4201 cgattacgtg cctctggagc ttactgagtt ggtcggcgac acgctgaccg ttgtgcacct
     4261 cgttcccata catagagaag aaccggcagc tccggttcag acggatcatc cgcccattgc
     4321 tgtggcgaat attcgaatcc ggtactcgac ccaggcagta actcaggaaa atatcggcac
     4381 gggcgtaaaa acttttcagg tacgcaacgt tggaaacatt ccttgcgaga acagcaaccc
     4441 gtgctccccg gacggcaagt ggaaagcctc cgtcaattcc atgtccatgg acgcaggcga
     4501 aggaaatgtt tttgaaaatg ccagagttcc gtgtatcgcg ggaccctgcc cgttcacgaa
     4561 agtggattac gatggatttt cccagggcgg cagaattata aaagtttcgg tgcgcaattg
     4621 gtcggacacc acgacttttc tatttcaagc ggaagtcttt cggccgcaaa tatacgacct
     4681 cgtgcaaatg acctacccaa ttattctggg cacgtccctg aattttacgt tgccgcccac
     4741 tgctgaaggg cctagcatcg aagcagaatt gaatggggaa aatattattt ttccactcgg
     4801 gccaattccg gtgttgagtt gggccgattg cgagcgaagc gtagcaaaaa ataaagcgcg
     4861 actttatcgg tgcgagctga attctggcta tcaatttcga taaacaagac acgaatacag
     4921 tacggaccgg caggggttga acattgcttg atggcaacag aacggtgcag acgatcggct
     4981 cgctggctgc ccaagcagtt ttgctcgttt cgatggttcg gaccccgctc acgctcagcg
     5041 aacttgcccc gccaattcct catcctgaaa ttgacaaccg ttccctgcaa gacgtgttga
     5101 cctacattga cggagcgtgg ggcacgttaa cgaggtccat ggagcaatgc cagagccttg
     5161 aggaccccaa gacagacagc gaggcgattg tgtatcttcc cgccggcatg gccgttccgg
     5221 aatcgttgtc gaaactccag gcgagctgcc gcgttcgcat cgagcacctt cccaatcaaa
     5281 tcacgaaagt tggtgagttc gacgccagca agattccggg cgaggggttg ctgtatctgg
     5341 aaaatcctta cgtggtgccg gggggacaat ttaacgaaat gtacgggtgg gacagctatt
     5401 ttattttgcg tggattaatc cgtgagcata gcttagaact ggccaaggga atggtggaaa
     5461 acttcttttt tgaaatcgag cattatggag ggatattaaa cgcgaaccgc acttactatc
     5521 tgactcgctc acaaccaccg ttccttacgt ctatgatcct ggccatttat gaagccgaga
     5581 aggcagaggg gaaaaccgat ctcgactggc tggagagagc gtatgcattc gctgaacgtg
     5641 atcacgaatc gtggaataaa accccacatc tagcgggaaa taccggacta tcgcgctatt
     5701 acgatttcgg ggatggcccg gtgccggaaa ttatgggcga tcccagccaa tattatcgcg
     5761 gcgccgccga tttttttctg gctcacgaga atttcgcgaa attaaatttg gttcgcgaag
     5821 gtgcgcatga gacgcaaaaa tccgcgcagg agcctcgctt ccctgtctta acttgcaacg
     5881 cgaaagcgga tgaggcggaa acaggaaagt gtgccccggc gtattgggtc gggctatctt
     5941 ccgaattcta taagggcgac cgcagcttgc gggagtccgg gtttgacgtc acgttccgct
     6001 tcggcccgtt cggagcagcc acgcatcatt ttgccgcagt ttgtttgaac agcctcctgt
     6061 acaaaacgga aatggacctc gtcgagatga gcaacatcct cggccatccc gaacgcgccg
     6121 agcactggaa acagacagcg gccgaccggc aaaaacgaat ggacaaatac ttttgggatg
     6181 ccgacgaagg aatgtttttc gattacaact ttgtggtcgg aaaacgttca cagtacgcgt
     6241 acgcgacgac gttttacccg ttgtgggcgg gcctagccac caaagaacag gcaaaagcca
     6301 tcgtgcaaaa tttgggagta ttcgagcagc atggtggact ggcgatgagc agaaccgaca
     6361 g
//
LOCUS       DASZMB010000260        21734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2736434, whole genome shotgun sequence.
ACCESSION   DASZMB010000260 DASZMB010000000
VERSION     DASZMB010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 21734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21734
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2736434"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VG272_12170"
     CDS             <1..880
                     /locus_tag="VG272_12170"
                     /inference="COORDINATES: protein motif:HMM:TIGR04018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HWF14488.1"
                     /translation="LYHYPVNMVFFTTPELLEIGDLPLTIASEKPTRSEALKYYRKCA
                     EHYELDLRLGHLVEKVEGVNGKFVVHTRDDHGNTAQFTGKKIAVATGYYDLPNRLGIP
                     GEDLPHVSHYYTEPHPFWRRNVVVIGGKNSAAEAALDLYRNGANVTLVHRRAELGSTI
                     KYWVRPDIENRIKAGQVKALFETHVQRIEEGRVVVSNHVGESTLPADQVFALIGYHPD
                     FQFLRSMGIMLNPQTNKPVMDPQSHESNVPGIFVAGVVTGGNHTSEIFIENGRFHGKQ
                     IVAALTGSRIDSAAKV"
     gene            919..994
                     /locus_tag="VG272_12175"
     tRNA            919..994
                     /locus_tag="VG272_12175"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:952..954,aa:Thr,seq:cgt)
     gene            1047..1454
                     /locus_tag="VG272_12180"
     CDS             1047..1454
                     /locus_tag="VG272_12180"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HWF14489.1"
                     /translation="MAQLDQTERTISAQLNRIYDPVESQDGYRALVDRLWPRGVSKAK
                     AKVDLWIKEIAPSDTLRKWFGHDPERWMEFQSRYHKELREKNDLIAEIRQLEKKYRTV
                     TLVYSAKDEMHSAGIYPRFLRNARSATACPPRK"
     gene            complement(1461..2513)
                     /locus_tag="VG272_12185"
     CDS             complement(1461..2513)
                     /locus_tag="VG272_12185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWF14490.1"
                     /translation="MQLGIDSFAAAISDPVTGLILSPVDRMQHLLEEIKLSDQVGLDV
                     FGIGEHHRAEFLDSAPAVILSAAAAQTKNIRLTSAVTVLSAADPVRVFQEFATLDLIS
                     NGRAEIVAGRGSFVESYPLFGLRLEDYDSLFAEKLDLLLNIRDNTHVHWSGKHRAALT
                     GQAIYPRPLQNPLPIWIGVGGTPESFARAGILGLPLMVAIIGGEPKRFRPLIDLYREA
                     GRRAGHSPDKLSVGLHAIGFLGETTEEAADDFFPGYAHTFTEIGKERGWPPTTRKQFD
                     AVRGPTGALLIGDAETVAKKILYVNEVLGGLSRVTFQMGVSTLPHPKMLRAIEILGTR
                     VAPLIRKELEATSARA"
     gene            complement(2595..3950)
                     /locus_tag="VG272_12190"
     CDS             complement(2595..3950)
                     /locus_tag="VG272_12190"
                     /inference="COORDINATES: protein motif:HMM:NF037981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HWF14491.1"
                     /translation="MAVNAPIKDAAPREIFDVGINDSLPPGQLLILGLQNVFGMTGMF
                     VFPGLLGRSFNLPSEKIAYLYGMTFAVCGVITVLQSIFLLRLPIIQGPYAGSFAALIS
                     VGHLQNGGLGAAFGSFFVASLIWCALTLPVRRFSFIGLFARFLRAPIISGMMVMLVII
                     QISNVALPNWIGLPSSPGFPVINILAGAVAIALVIGVTLWGGTRLRRGAILAGLAAGT
                     ACYAIFRPISFAAVANAPVLVAPLWFPFGFGVRADLVIVFLLVLIPPGMGSMAMYQMV
                     ADWGAENLPAERMAQGVFGMAIGSVLAAIVGGLSTIAYPDNIGMLRATRVGSRYATLA
                     AGILLITLGGFIKFDMLLVVVPMPVISATATLLFGIVFMHGVHMLANVDWDERKFMVA
                     GLALLVGLGGLFISPEVLQSMPLMVRLIVQQPVISGGLTLVVLHSILCAIPPANESTR
                     T"
     gene            complement(4059..5711)
                     /locus_tag="VG272_12195"
     CDS             complement(4059..5711)
                     /locus_tag="VG272_12195"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HWF14492.1"
                     /translation="MSGNEQRSNLRPSLDFQSHLARLEKLGLLIRVDRAINKDTELHP
                     LVRCQFLGGIPEEERRAFLFTNVLDGHGRRYDIPVVVGALAASPKIYAAGMGCAVEQI
                     GAAWDHAIANPIPPTLVNSAPCQEVILRGDELQKTGGGLASLPVPISTPGFDAAPYLT
                     ATLCITRDPETGIRNMGTYRGALKATDRLGVRMSSRIGGAGGYLHWLKYKKRKEPMPC
                     AIVIGCAPVVVYTGPQKFSANVDEMNIAGGAAGAPIRTVKCVTLELEIPADSEIVIEG
                     LIDTDLLEPEGPFGESNGYVALEDYNMIMQVTAITHRRSPVFASILSQVTPSESSVIK
                     KVAYEPAFLAHLRDQLSIKGIRRVVMHEPLSNVRPIIFLQFANDTPRTEVWRGLQGAT
                     AKQADCGKIVIAVSEDIDPTNTDAVLWSLAYRSNPAEDVHIEPYRSRGHAPKSGSQAL
                     DSTMLVDATLKSSMPPLALPAREHMERAQAIWKELGLPPISVRPPWHGYSLGDWSDAW
                     EHFARKAVAGEWAENGSNTATRRREGLTPETPVRNVEGHEKT"
     gene            complement(5881..7407)
                     /locus_tag="VG272_12200"
     CDS             complement(5881..7407)
                     /locus_tag="VG272_12200"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HWF14493.1"
                     /translation="MRTSLGVAGTLLPFAVLFSAFLIFKMDAFRASLSAWVVELFLVL
                     VFYRMSFVKSLEASVWGNLSMWTGFLVLYMGQLFGQAYRSTGLLEILLESIRSMLPSR
                     DVEGRSVALVTLVAGFIGAFNGFATYPVTIPGLVDIGFDGVQATTSYLVYFSWTIPFN
                     SLFIAPNISNAASHVAVVDIVRVGGLLTIPLVLLSLLGFLKILGFRFFAWKTQALFWI
                     TGLANVIAIVLFTQIWPAYYILMMIGGAVISLAGIYIYGRLAKCSEAAQLQNAEQATP
                     SYSGMGLFKAYAPLLLGVVIVMLTRAHSVSEWLDHFQFGLSAWGYNRISINVFTTAGF
                     YIFVTALACYPFRSKDANAGKDFVVASKRSVRSLSTLAVGSAAVYLMVDSGQIARLGK
                     ILASGGKFVYSSCYPLVAFLGGMAFGQGLPGDFLFSRMQVSIAPTLGIPLMVLVGIIL
                     VVTMGPPNALKPTQIAFSASLANVKGRDGEIFRICLPWVLLQLVVTAVFSVILVYWWK
                     "
     gene            complement(7569..8429)
                     /locus_tag="VG272_12205"
     CDS             complement(7569..8429)
                     /locus_tag="VG272_12205"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HWF14494.1"
                     /translation="MTTLSRFEIHQPATVQEASQMIEHYGDDAGIYAGGTELLLAMKH
                     SALNYRHLIDLKVVPGLNSIEVRNGALEIGATSTHRSIERSAIVQQHQPVLAEMESHV
                     ANVRVRATGTLGGNLCFAEPHSDPATLLLVLGGAVRIESMTGAREIPVSELIGGAYSS
                     NLQPGEILTKITVPCAKPVHRAAYMKFQVHERPTLGVGLWLDTPDGGRTIASARVAVG
                     CVCPFPTRGLDAEKLLIGSREEVEKHLPEAADAIADAADLVDDHEGSAEYKRHLIQVF
                     LQRVFRKAFD"
     gene            complement(8557..10830)
                     /locus_tag="VG272_12210"
     CDS             complement(8557..10830)
                     /locus_tag="VG272_12210"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HWF14495.1"
                     /translation="MKPLNEFQIVNHSVPRGDGIPKVTGGAIYASDMVLEHMAWAKIL
                     RSPFARARIISIDTSEAKKQPGVIGVLTGYDLKGLHPYYGHGVKDHPLIAIGQVRFVG
                     EPVAAVIAETELDAQEALGKINVEYEEMTAILDVDTAMAEGTPRIHETDFADGSFRGF
                     DDFPGAAGNICQAVHVGWGDVDAAFASAAHIAEGEFYFPMVYAYAMEPYVAIADFDAK
                     GHLTVYSSAQHPFMVRHDVAHVFGLPLNSVRVIVPYIGGGYGSKSYTKIEPLVSACSW
                     KAGRPVKLQLSVEEAFLTTRGDDARVRIRTATDANGNLVARQATIHLNTGAYAENSPM
                     VCRKAANRIIGPYRIPNVKIDCFAVYTNTVPASSYRGLGGAQVTFPGESQMDELAEKA
                     SCDAIEFRLKNLAQSGESIHPGMRPIDADIPGDVREAAKMLHMDRPVAPGHGRSVCCS
                     ASDAGAHPVTLAMVQVYADGAVSVLSGSTEIGQGSHTVLTQIAAEEMGVPLEKVRLIG
                     SDTAVTLFERSTGASRTTTLMGRAVLEACREAIAQIKVMAEEVFAAPAEKLVEERGGL
                     RCGETFLTWAQILENYFKMEGCSIVGRAYLRKAGDLTLIPVFWEIGVVGAEISLDEET
                     GTITLDSLVTIGDVGLAINPAMTEGQDLGAATMGLGAGLFEELIYDGQQLMNGSMLEY
                     RVPRFSDLAKNVDMKLVQNRDGVGPYGAKGGGEGAVNPISPCIANALYRATGVRMRTL
                     PLTPERVWKALQKKKKS"
     gene            complement(10827..11300)
                     /locus_tag="VG272_12215"
     CDS             complement(10827..11300)
                     /locus_tag="VG272_12215"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HWF14496.1"
                     /translation="MNIGFVLNGKPTTLEIEQRESLAEVLRGHCRLTGVKISCEAQVC
                     GSCTVLVDGLPVSACTLLAYEARGKRVTTIEGVSDESGKLHPLQQAFLDHFSFQCGFC
                     TPGMILAAKAMLDKNNNVTEDEIVAHMNGNLCRCTGYLPILKAIRQAADEMRGQK"
     gene            complement(11352..11828)
                     /locus_tag="VG272_12220"
     CDS             complement(11352..11828)
                     /locus_tag="VG272_12220"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HWF14497.1"
                     /translation="MKLTEELPLNASIDQVWKLLRDTPRLTGLLPGVENVARLMDDIN
                     EAYSAKVSEKIGPFKITMNLEVRITETVEPSLLKVSLKGTDSMSLNRMTGSMQVSLIA
                     APCGTQMHFEASIEVLGKIATLGAVPIRRRTTQVFAEFAKNIQGEFVENSNSTSNS"
     gene            complement(12443..13288)
                     /locus_tag="VG272_12225"
     CDS             complement(12443..13288)
                     /locus_tag="VG272_12225"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HWF14498.1"
                     /translation="MLIRLGYDIEFNIPHPVTVVALLNVHPSRAPDLLEPDELSIEPK
                     VKTETYFDRYANRCTRFLAPEGKLRIWNSTLIKDSAEKDPINCQAQQHPVIDLPAENL
                     TFLMNSRYCEVDRLSNIATDLFGNTAPGWGRVQAIVNWIHGRVQFGYHHARSTRTALD
                     VYTERVGVCRDFQHLAIAFCRCMHIPARYATGYLGDIGVPASTAPMDFSAWFEVYLEG
                     RWWTFDARHNEPRIGRVLMATGRDAADVAITTSFGMAELAKFTVVTDEVNDAASGNDF
                     CSQGA"
     gene            complement(13497..15023)
                     /locus_tag="VG272_12230"
     CDS             complement(13497..15023)
                     /locus_tag="VG272_12230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HWF14499.1"
                     /translation="MKLTKIMTPIFRHAAVFLLATSSLLAGCMVGPKYHTPTATTAPA
                     YKELTPADYKNTDGWKVAQPKDDALRGKWWEIFNDPELNELEEKVNVSNQTIAAAAAN
                     FFAARALVKESRAQLFPTVSVGPSIGLQRSPSIGKGVTTSSTGTASASSSSGTFAEYS
                     LPFDASWQPDLFGRIRNTVRAAAYGAQATAADLENTRLTMQTEVAVDYFDLRGQDSLK
                     QLLDSTLDAYQKSLDLTTALYQTGIGAEEAVAQAETQLEATKAQDTAVGIQRAQFEHA
                     IAMLTGQPASTFSIPVKPLIPNPPAIPIGVPSQLLERRPDVASAERLVAQANAQIGIA
                     RAAYFPTVTLSGAAGLESTSITTLFNWQSRFFSIGAAGVESLFDAGLRRATVEQFQSQ
                     YNETVANYRQAVLVAFQQVEDNLSTLRILSAEIQDQDKAVNSAQRTLALATDRYQLGI
                     DPYLNVITAQTSLLSNQEAAVNLRIQQMTASGGLIEALGGGWDTSQLPSPGQLVSKAP
                     "
     gene            complement(15040..18156)
                     /locus_tag="VG272_12235"
     CDS             complement(15040..18156)
                     /locus_tag="VG272_12235"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWF14500.1"
                     /translation="MNISEPFIRRPVATTLLTIAIALAGAVAYGVLPVAPLPEVDFPT
                     ISVGAALPGASAEIMASSVATPLERQFGHIAGVTEMTSASSLGTTSITIQFDLSRKID
                     GAARDVEAAINAARTYLPANLPGNPTYRKVNPADAPIMILGLTSDKFGPDKMYDEAST
                     VIQQKLSQIQGVGQVTVGGGALPSVRVDANPMQLANYGLTLANLQSVLSLQNSNLAKG
                     QITDGNVTVDILANDQISHAADYKPLVVGYKNGAAIRLSDVAEVTDSVQNIRTGGYLN
                     GKRAVTIIIFRQPGANIIDTVDRIRNQLPSIQATVPLGIDTTIVLDRTTTIRASVSDV
                     ERTLMISIALVIVVVFIFLRNGRATLIPAIAVPVSLVGTFAVMYLFGYSIDNLSLMAM
                     TISTGFVVDDAIVVMENISRHLENGMAPFAAALKGAQEIGYTVFTISVSLIAVFIPLL
                     LMGGIVGRLFREFAVTLSTSIVVSMAISLTTTPMMCAYLLKNERKEKHGRIYMASERF
                     FDWVLSVYRSSLEWVLANPIPTLVILLLTILLNVVLIVKIPKGFFPEEDTGAIVGAVR
                     GPQDSSFPAMNDSIQRIGAVIKNDPAVNNVIGFSGGNGATNTGFIYATLKPLKQRTAS
                     ASQVINRLRPQLARLPVASAFLQAVQDLRIGGRSSNAMYQYTLQSDTQEDLTKWGPIL
                     LAQMRKLPGLQDVSADQQNGGLDELLHFDRATAAKLGITAQSLDSSLYSAFGQSEVSI
                     IYTQLNQYYVVLEVAPQYWQSPEGLKYIYLHTPSSKSSIPLLTVAKSQANTTPLAINH
                     TGLFPSVTLSFNLAPNVSLSQAALSIDQMQRRLGTPSTMQGFFAGTLLAYQQSLGTEP
                     VLVLTALLAVYIVLGILYESLVHPLTIISTLPSASVGAMLALILFKVDLNVISIIGIV
                     LLIGIVKKNAIMMIDFALQEERLEGKTPFDSIFEACMLRFRPILMTTMAALLGALPLA
                     MGTGTGYELRRPLGITIVGGLILSQMLTLYTTPVVYLTFDRLRLRMQGKRKTVFGRDP
                     GPAPSPAD"
     gene            complement(18153..21473)
                     /locus_tag="VG272_12240"
     CDS             complement(18153..21473)
                     /locus_tag="VG272_12240"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HWF14501.1"
                     /translation="MSPSRPFILRPVATALLMAAILLVGIVSYKQLPVSALPEVDYPT
                     IQVLTFYPGASPNVVSTTVTAPLERQFGQLQGLSQMTSTSSGGTSVIVLQFNLSLAID
                     VAEQEVQSAINASQSLLPSILPAPPTYSKTNPADAPVLTLAITSDSMPLSQVEDLVDT
                     RLAPKISQLSGVGLVSISGGQKPAVRIQANPTAMSSYGINMEDLRTALTQSSVNAAKG
                     NFDGVRQDYQIDANDQLITSNDYKNVVVAYRNGAPVMLTDVANIVNGVENNNQAAWMN
                     QTPAVILNVQRQPGANTISVVRSIKNLLPQLKANLPTGIQVTTLTDLTTSIEASVADV
                     EFELMLTIGLVVLVIFLFLRSLYATIIPSVAVPLSLVGTFGAMYILGYSLDNLSLMAL
                     TISTGFVVDDAIVMIENISRYIEAGDPPMEAALKGAEQIGFTILSLTVSLIAVLIPLL
                     FMGDVVGRLFREFAVTLAVTIVVSAVVSLTLTPMMSARILRHEPKEKQGRIYQATERA
                     FEAMIEFYGRTLKFVLGYQTITLLVAVATLLLTIFLYIIIPKGFFPVQDTGVIQGISQ
                     APQVIGSKAMAEKQLELTKVVMADPAVESVSSFIGADGTNTTTNSGRMSINLKPIEQR
                     NISASNLIRRLTAKVNQVQGIQLFMEPVQNITVDDRVSRTQYQYTLEDPDANELNDWT
                     NRFVTELKKLPELEDLATDQQLGGLAVNLVIDRVTASRLGIAPATIDNTMYDAFGQRQ
                     INTMYTQVNQYHVILEAQPQFQLDPNKLNHLFIQANASSGASGTGASTSFASSGSSSA
                     GSNALTTSALYTPSASSLTPPQNALSANASTSSNRTTSAALTTSSAMSNAVPLSTFTH
                     FENTTEPLSINHQGQFPAITVSFNLASNASLGSAITAVNKVQENMHMPASLQASFQGT
                     AASFTNSLSNEPLLILAALVTVYIVLGVLYESFVHPITILSTLPSAGVGAFLALILFH
                     QDLNVVAIIGIVLLIGIVKKNGIMMVDFALEGERNEGKNATDAIYEACLLRFRPIMMT
                     TMAALLAGLPLALGRGFGSELRRPLGIAMVGGLLLSQLLTLYTTPVIYIFFDNLGRRF
                     SHKPNNATAGDAQRPEPA"
     gene            complement(21470..>21734)
                     /locus_tag="VG272_12245"
     CDS             complement(21470..>21734)
                     /locus_tag="VG272_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14502.1"
                     /translation="TLQNVTLVPSSAIQQNGQASFVYVIQNNSAHMRSVKPGVTNAGM
                     TQVDGINPGDVVANSSFDKLQDNVPVVVSGTPAPNGLPRSNAP"
BASE COUNT         5155 a         5953 c         5811 g         4815 t
ORIGIN      
        1 tctgtaccac tatcccgtca acatggtgtt tttcacgacg cctgagttgc tggaaattgg
       61 cgatctgcca ctgacgattg cctccgagaa gcctacgcgc agtgaggcgc tcaagtatta
      121 ccgcaagtgc gctgaacact atgagctgga tttgcggctg ggccatcttg tcgaaaaagt
      181 ggaaggcgta aacggaaaat ttgtggtgca tacgcgcgat gatcacggaa ataccgcgca
      241 attcaccggc aagaagattg ccgtagctac cggttactac gatcttccaa atcgcctcgg
      301 gattcccgga gaggacctgc cgcatgtttc gcactactac acggagccgc atcctttttg
      361 gcggcgaaac gtagttgtga tcggcggaaa aaactcagca gcggaagccg cgctggatct
      421 ttatcgcaat ggcgcaaatg taactctggt tcatcgccgc gcggaactcg gctcaaccat
      481 aaaatattgg gttcggccag acatcgaaaa ccgcattaag gccgggcaag tcaaggcact
      541 ttttgaaacg catgtgcagc gcattgaaga aggtcgcgtc gtcgtttcta atcacgtggg
      601 tgaatcgacg ctgcctgcgg accaggtttt tgccctaatc ggatatcatc ccgattttca
      661 gtttctgcgg tccatgggga tcatgctcaa tccgcaaacc aataagcccg tcatggaccc
      721 gcaatcccac gaaagcaatg ttcccggaat tttcgtggcc ggggtcgtga ccggcggcaa
      781 ccacacaagc gaaattttca ttgaaaatgg gcgctttcac ggcaagcaaa ttgttgctgc
      841 tttgacaggt tctcgcatag attctgccgc taaggtttga cactattttc cacacgatgc
      901 tagagtgatt gtcaccgagc cgaagtagct cagttggtag agcagtcgat tcgtaatcga
      961 caggtctccg gttcaagtcc ggacttcggc tccaactact tagccgtcaa cactggcgtt
     1021 tcgcggattt tcaaggtggg aagaagttgg cacaacttga tcaaaccgaa cgcacaatct
     1081 ccgcacaact aaaccgcata tatgatccgg tggaatcgca agacggatac cgcgcgcttg
     1141 tggacaggct ttggccacgc ggcgtgtcaa aggccaaagc caaagtcgat ctctggatca
     1201 aagaaattgc tccctcagac acacttcgca aatggtttgg gcacgatcct gaacgttgga
     1261 tggaatttca gagtcgctat cacaaagagt tgcgggagaa aaacgacctg atagcggaaa
     1321 tcaggcagct ggagaagaaa taccgcactg tcactctagt ttattccgcc aaagatgaaa
     1381 tgcacagcgc ggggatctat ccccgttttt tgagaaatgc gcgaagtgcg accgcttgcc
     1441 ccccaaggaa atgacccaca tcaggcacgc gcggaagtag cttccaattc tttccggatc
     1501 aaaggcgcaa cgcgcgttcc tagaatttcg attgctcgga gcatctttgg atgcgggagc
     1561 gtcgagacgc ccatttgaaa cgtgacgcgc gaaaggccgc caagaacttc atttacgtac
     1621 agtatttttt tagcgaccgt ttcagcgtcg ccgattagca gcgcaccggt gggccctcgc
     1681 acagcatcaa actgtttgcg tgttgttgga ggccagcctc gctctttgcc aatctcagtg
     1741 aatgtatgcg cgtatccggg aaagaaatcg tccgccgctt cttccgtggt ttctcccaaa
     1801 aatccgatgg cgtgaaggcc cacggaaagt ttgtcgggcg aatggccagc cctgcggcct
     1861 gcttcgcggt aaaggtcgat caaagggcgg aaccgtttcg gctcgccgcc gataatcgca
     1921 accataagcg gaaggccgag aattccagcg cgggcgaagg attcaggcgt gccgccgaca
     1981 ccaatccaaa tgggtagcgg attttgcagc gggcggggat aaatcgcctg acccgtgagc
     2041 gcggcgcgat gcttgccgga ccaatgcaca tgcgtgttgt cgcgtatgtt taagagaagg
     2101 tcgagctttt cagcgaaaag ggaatcgtag tcttcgagac ggagaccgaa aagcggatag
     2161 gactctacga acgaaccgcg gcccgcaacg atctccgcac gaccattcga aatcaaatca
     2221 agcgtggcaa attcctgaaa aactcggact ggatcagcgg cactcaggac agtcaccgcg
     2281 ctggtgaggc gtatgttctt tgtttgcgca gccgcagccg acaaaatcac ggcgggggcg
     2341 gaatcgagga actcggcacg gtgatgttcg ccgatgccaa aaacgtcaag gccaacctga
     2401 tcggagagct tgatctcctc caataggtgt tgcatgcgat ctacggggct gagaatgagg
     2461 cctgtgactg gatccgagat ggccgctgcg aagctatcga tgccaagctg cattttgcga
     2521 ctcctgtggt ttcgtctgga attagatgat cgacttgcaa attggtcgca agttggtgtt
     2581 gagcttatgg aagcctaagt gcgcgtactt tcattcgcgg gcggaattgc gcaaaggatc
     2641 gaatgtagga cgacgagcgt gagccccccg gaaatcaccg gttgttgtac gatgagcctg
     2701 accataagcg gcatgctctg cagcacttcg ggcgagataa acaggccgcc aagccccacc
     2761 agaagcgcaa gacccgccac catgaatttc cgctcgtccc aatccacatt ggcgagcata
     2821 tggacgccat gcataaagac gatgccgaac agcaaagtgg ccgttgcgga gatgaccggc
     2881 atgggcacga cgacgagcaa catatcgaat ttaatgaatc cgccgagtgt gatcaaaaga
     2941 attcctgccg cgagcgtcgc gtagcgcgag cccacgcggg tggcgcgcag catgccgata
     3001 ttatccgggt acgcgatggt gctcagccca cccacgattg cggccaacac ggatcctatg
     3061 gccattccga aaacgccttg cgccatccgc tccgccggaa gattttctgc tccccaatcc
     3121 gcaaccatct ggtacatggc catcgatccc attcctggcg gaatcagtac gagcagaaaa
     3181 acgatgacga ggtcggcgcg cactccaaat ccgaagggaa accatagtgg cgcgactaga
     3241 actggggcat ttgccaccgc tgcaaaagaa atagggcgaa aaattgcgta gcaagctgtt
     3301 ccagctgcaa ggccagcaag aatagctccg cgccgcaggc gtgttccgcc ccacaaagtc
     3361 acgccgatga cgagcgcgat ggccacggct cccgcaagga tatttatgac tggaaatccg
     3421 ggacttgagg gcagtccgat ccagttcggc aacgctacat tcgatatctg gatgatgacg
     3481 agcatcacca tcattccgga aatgatcggc gcgcgcagga aacgcgcgaa caggccaata
     3541 aaactgaatc ggcgcacagg caacgtgagt gcgcaccaga tcagcgacgc gacaaagaat
     3601 gatccaaaag cagcgcccaa tccgccgttt tgcaaatgtc ccaccgagat taatgcggcg
     3661 aagctgccag cgtacggtcc ctggataatc gggagacgaa gaaggaatat ggattgcaga
     3721 acggtaataa cgccacacac tgcgaacgtc atgccataca gatatgcaat tttttcagac
     3781 ggcaaattaa atgaccgtcc cagaagtccg ggaaatacga acatgccggt cattccgaaa
     3841 acattctgca aaccaagaat cagaagctgg ccgggtggaa gagagtcgtt gataccaacg
     3901 tcgaagattt cgcgtggggc tgcatccttt atcggcgcgt tgacggccat gacatccttt
     3961 acgcgaaatt tctatccaga gtgtgaagcg acgctttaaa atgcgtttcg catgcttctc
     4021 gaaatctgtt catcggtaaa tgcttctgtg tcatggttct acgttttttc gtgaccctca
     4081 acatttcgta cgggagtttc cggagttagt ccttcgcggc gtcgcgtcgc cgtattcgag
     4141 ccgttctcgg cccactcgcc tgcaacggcc ttgcgcgcga aatgttccca ggcatcgctc
     4201 caatcgccca gcgaatagcc atgccaggga ggacgaacgg agatcggcgg aaggcccagc
     4261 tctttccaga ttgcctgagc gcgctccatg tgttcgcgcg cgggcaacgc aagaggcggc
     4321 atcgagcttt tcaatgtcgc gtcgacgagc attgtcgaat ccaaagcttg cgagccggat
     4381 ttcggtgcat ggccacgcga ccggtagggc tcaatatgca catcctcggc aggatttgag
     4441 cggtaggcga gcgaccacag cacagcatcc gtgttcgtcg ggtctatatc ttcgctgacg
     4501 gcaatcacaa tttttccgca gtcggcttgc ttcgcggtcg cgccctgcaa cccacgccac
     4561 acttccgtgc gcggagtgtc attcgcaaat tgcagaaaaa tgatcgggcg aacattgctc
     4621 agcggctcgt gcatcaccac gcgtcgaatt cctttgatcg acaattgatc gcgcaaatga
     4681 gcaaggaacg ccggttcgta ggcaactttt ttgatcacgc tggattcgct gggtgtgact
     4741 tggctaagaa tggaagcgaa caccggtgag cgccggtgcg tgatcgccgt gacctgcatg
     4801 atcatattgt agtcttcgag cgcgacatac ccgttgctct cgccaaacgg tccctcgggc
     4861 tcaagcaaat ccgtgtcgat cagcccttcg ataacgatct cggaatccgc cggaatttcc
     4921 aactcgagcg tgacgcattt taccgtgcga atcggcgcgc ccgccgctcc tccggcaata
     4981 ttcatttcat ccacattggc ggaaaatttt tgtggtcccg tataaaccac cacaggtgca
     5041 caaccaatta caatcgcgca aggcatcggc tctttccgct ttttgtattt cagccaatgg
     5101 aggtatccac cagctcctcc aatgcgcgaa gacatgcgca cgccaagccg gtccgtagct
     5161 ttcagcgcgc cgcgataggt tcccatgttg cgaatcccgg tctcgggatc gcgtgtaatg
     5221 caaagcgtgg cggtcagata gggagcagcg tcaaagccgg gagtagagat cggaacgggt
     5281 aggctcgcca aaccaccgcc cgtcttttgc agttcatcgc cacgcaggat cacttcctgg
     5341 caaggcgcgg aattcacgag cgtgggagga attggattgg cgatcgcatg gtcccatgct
     5401 gcgccaatct gctccaccgc gcatcccatg ccagccgcgt aaattttcgg cgaagcagca
     5461 agcgccccaa ccacaacggg aatgtcatag cgccggccat gaccatcgag aacgttcgtg
     5521 aaaagaaagg ctcgccgctc ctcctccgga attcccccaa gaaattggca acgcaccagc
     5581 ggatgcaact ccgtgtcctt attgatcgcc cgatccacgc gaatcagcag tccaagtttt
     5641 tcgagtcgag ccagatgcga ttggaaatcg aggctaggcc gcaggttgga tcgttgttcg
     5701 ttcccgctca ttgcaagact cctataaaaa attgctcgaa ggccccggcg ttgtagctcg
     5761 cctcttcaga ggcgagggtt tagatgtcac gcgcggcaga aatgtcggtg cgtctaaacc
     5821 cccgcctctg aagaggcggg ctacaaaaaa tacactgcac atcccgctcg acattcttca
     5881 tcacttccac caatacacca gaataacgga aaaaactgcg gtcactacaa gttgcaatag
     5941 cacccacggc aagcatatcc gaaaaatttc gccgtcccgt ccctttacat tagccagcga
     6001 agcgctgaaa gcgatctgcg tcggcttcag cgcattcggc ggccccatgg tcaccaccaa
     6061 aataattccg accagaacca tcagggggat gcccaacgta ggcgcgatgg aaacctgcat
     6121 ccgcgaaaaa agaaaatctc ccggtagtcc ttgaccaaac gccattcccc ccaaaaacgc
     6181 caccagcggg tagcaggacg agtacacaaa ttttccgccg ctcgccagaa ttttgccgag
     6241 ccgcgcgatt tgtccggaat ccaccatcag atataccgca gcgcttccaa cagcaagtgt
     6301 tgaaagactt ctgacgcttc gtttactggc gaccacaaaa tccttcccgg cattggcatc
     6361 tttggagcga aaggggtagc aggcaagcgc cgtaacaaaa atgtaaaagc cagccgtcgt
     6421 aaagacattg atcgagatgc gattgtatcc ccaggcggaa agtccaaatt gaaaatgatc
     6481 gagccactcc gaaacgctgt gcgctctcgt tagcatgacg atcactactc cgagaaggag
     6541 cggcgcatac gccttgaata agcccattcc ggaataagaa ggcgtggctt gttctgcatt
     6601 ttgtaattgt gctgcttcgc tgcatttcgc gaggcgtccg tagatataaa ttcccgcgag
     6661 ggaaatcacg gcgccgccaa tcatcatcaa aatatagtaa gcgggccaga tctgagtaaa
     6721 cagaacaatg gcgatgacgt tggcaaggcc tgtgatccag aagagagcct gcgttttcca
     6781 cgcaaaaaag cggaatccta aaattttcaa gaaaccaagc agggaaagca gaaccagcgg
     6841 aatggtaagc agtccaccca cgcgcacaat gtccacaacc gccacatggc tcgcggcatt
     6901 ggaaatattt ggcgcgatga agaggctgtt gaagggaatc gtccacgaga aatagaccag
     6961 ataacttgtg gtcgcctgaa cgccatcgaa accaatatcg acaagaccgg gaattgtaac
     7021 gggataagtg gcgaatccgt tgaacgcgcc aatgaaaccc gcaacgagcg ttacgagcgc
     7081 gacggaacgc ccctccacat cacggctcgg caacattgag cgaatcgatt cgagtagaat
     7141 ttccagcagt ccggtgctgc ggtacgcctg tccgaagagt tgccccatgt agaggacgag
     7201 gaatcctgtc cacatggaaa gatttcccca caccgacgct tccaggctct ttacgaaaga
     7261 cattcggtaa aacaccagca cgagaaataa ttccacgacc catgctgaaa gactggccct
     7321 gaacgcatcc attttaaaaa ttagaaatgc ggagaacagc acggcgaatg gcagtagcgt
     7381 gccggcaact cccaagctgg tcctcaaaga attcctccgc gggaatacgg aaaaactgaa
     7441 atgtttcgat atgaacgcgg gcacagtaga tttgttctcc gcgtttgtca acgcgcgcct
     7501 gaaagacagg gtttaacaca gaggtcatag cgccccctgg agaaacccag agatgttcca
     7561 ccagcacgtt agtcgaatgc tttacggaac actctttgca agaaaacctg gatcaggtgg
     7621 cgcttatatt cggcgctgcc ttcgtgatcg tcgaccagat cggcggcgtc cgcaatcgca
     7681 tcggctgcct cgggcaagtg tttctcaact tcttcacgcg aacctataag taatttttca
     7741 gcgtccaggc cgcgtgtcgg aaatggacag acgcaaccaa ctgcaactcg cgcggacgca
     7801 atcgtgcgcc caccgtcagg cgtatccagc cacaggccca cgccgagcgt gggacgctca
     7861 tgcacttgga atttcatgta ggcggcgcgg tgaacaggct tcgcgcaggg aacggtaatc
     7921 ttcgtgagga tctcgccggg ctgcaaattg ctggagtatg ctccgccgat cagttcactg
     7981 acgggaatct cgcgtgcgcc ggtcatactc tctatccgca ctgcacctcc caacaccagc
     8041 aacaaagtcg cgggatccga atgcggctca gcgaaacaaa ggttgccccc cagcgttccc
     8101 gtggcccgca cgcgcacatt ggccacatga gattccattt cagccaaaac aggttgatgt
     8161 tgctgcacaa tcgccgagcg ctcaatactg cgatgcgtcg aagtcgctcc aatttccagc
     8221 gcgccgttgc ggacctcgat ggaatttaac ccgggaacca ctttaaggtc gatcaggtgg
     8281 cgatagttca gggctgaatg cttcatcgcg agcagcagtt ccgtgccacc tgcatagatt
     8341 cctgcgtcat cgccgtaatg ctcgatcatc tgcgaagctt cctgcaccgt cgctggctga
     8401 tgaatttcga aacggctaag tgtagtcata aattcctagc gaaaaaatcc ttgtgtagcc
     8461 gccatttgtt acctttgtag ctcgcctcat cagaggcggg ggtttacatg cacatacatt
     8521 taaacccctc gcgtctgaag aggcgagcta cagcgcttaa cttttctttt ttttctgcag
     8581 cgctttccaa acgcgttcgg gcgtcagtgg caaagttctc atccgcactc ccgtggctcg
     8641 atacaacgca ttcgcgatac atggtgaaat cggattgacg gcgccttcac cgccaccctt
     8701 cgcgccatat gggccgacgc catcgcgatt ctgcaccagc ttcatgtcca cgtttttggc
     8761 caaatcggaa aagcgcggca cgcggtattc gagcatcgac ccgttcatca actgctgccc
     8821 gtcataaatt aattcttcga acaagcccgc gccgaggccc atggtggccg cacccaggtc
     8881 ctgcccttcg gtcatcgcag gattgatcgc caggcccacg tcaccgatgg taacgagcga
     8941 atccaacgtg atcgttccgg tttcttcgtc cagcgaaatc tccgcgccta caaccccgat
     9001 ttcccagaac actgggatta gggtcagatc gccagccttg cgaaggtagg cgcggccaac
     9061 gatgctgcag ccttccatct tgaaatagtt ttcgagaatc tgcgcccatg tcaggaatgt
     9121 ttcgccgcaa cgcaaaccgc cgcgttcctc caccagcttt tccgcaggcg ctgcgaagac
     9181 ttcctcggcc attaccttta tttgcgcgat tgcttcgcgg cacgcttcca gcacggcgcg
     9241 gcccatcagc gtcgtcgtgc ggcttgcgcc cgtcgagcgt tcgaacaagg tgaccgccgt
     9301 gtccgaaccg atgagccgga ctttttccag cggcacgccc atttcttccg ccgcgatttg
     9361 cgtaagcacc gtatggctgc cctggccgat ctccgtgctg ccgctcaaga ccgaaacagc
     9421 gccatccgcg taaacctgca ccattgccag ggttaccggg tgcgctccag catcgctcgc
     9481 cgagcagcac accgagcgcc catgcccggg cgccacagga cgatccatat gcagcatttt
     9541 tgcggcttcg cgaacgtcgc cgggaatatc ggcgtcgatg gggcgcatgc cgggatggat
     9601 cgactcgcca gattgcgcca gatttttcag ccggaattcg atagcgtcgc agcttgcttt
     9661 ttccgcaagc tcgtccatct gcgattcgcc ggggaaggtg acctgcgcac cgccaagacc
     9721 tcgataggaa ctcgcaggta ccgtgttcgt gtacacggcg aaacaatcga tcttcacatt
     9781 cggaatccgg tacggcccga tgatgcgatt ggcggccttt ctgcacacca tggggctgtt
     9841 ttccgcatat gcgcccgtgt tcaggtgaat tgtcgcctgc cgcgctacca gattcccatt
     9901 cgcgtcggtc gcggttcgaa ttcgaacgcg cgcatcgtct ccgcgagtcg tgaggaaagc
     9961 ttcttcgaca cttagctgca gcttaacggg acgtcccgct ttccacgaac aagcagaaac
    10021 gagcggctca attttcgtgt aagatttgct gccgtaaccc ccgccaatgt agggcacgat
    10081 cacgcgcaca ctgttgagcg gcaagccaaa aacatgagca acgtcgtggc gcaccatgaa
    10141 gggatgctgc gccgaggaat acactgtcag gtgtcccttg gcgtcaaaat cggcgatggc
    10201 cacgtacggt tccatcgcgt aggcataaac catggggaag tagaattcgc cttcggcgat
    10261 gtgcgcggca gaagcgaatg ctgcgtctac atctccccag ccgacgtgca cggcctggca
    10321 aatgtttcct gccgcgccgg ggaaatcgtc gaagccgcga aaggagccgt cggcgaaatc
    10381 cgtttcgtga atgcgcggcg ttccttccgc catggcggtg tccacgtcga gaatcgccgt
    10441 catctcctcg tactcaacgt tgatctttcc cagcgcctcc tgcgcgtcca attctgtttc
    10501 cgcgatcaca gccgcgaccg gctcgcccac gaagcgcact tgtccgattg cgatcagtgg
    10561 atggtctttc actccatggc cgtagtaggg atgaagccct ttcaagtcat atccggttag
    10621 cacgccaatt acgcccggct gtttcttagc ctcggaagtg tcgatggaaa taattcgggc
    10681 gcgcgcaaac gggctgcgca aaatttttgc ccatgccatg tgctcgagaa ccatgtcact
    10741 cgcgtagatc gcgccgccgg ttactttcgg aatgccgtca ccgcggggga cggaatgatt
    10801 gacgatttga aattcgttga gcggcttcac ttttggcctc gcatttcgtc cgcggcctgg
    10861 cgaatcgcct tcaggatcgg caagtacccg gtgcagcggc acagattgcc attcatgtgc
    10921 gcgacgatct cgtcctctgt cacattgttg tttttgtcca gcatcgcttt cgcggccaaa
    10981 atcattccgg gtgtgcagaa tccgcattga aaagaaaaat gatccaggaa tgcctgctgc
    11041 aagggatgca gctttccaga ttcgtccgat acgccttcaa tcgtagtgac acgcttcccg
    11101 cgcgcctcat aagcaagcag cgtacacgcg ctgacgggca atccatcgac caacaccgtg
    11161 cagctgccgc acacttgcgc ctcgcaggaa attttcacgc ccgtcaagcg gcaatgtcct
    11221 cgcaaaactt cggcgagcga ctcgcgctgc tcgatttcta acgtggtcgg ctttccgttt
    11281 agcacgaaac caatattcac aacttctcct tgctcattgc gtaagatctc tgcgaatcct
    11341 aaattctatt cctacgagtt cgaagtcgag ttcgaattct caacaaactc tccctgaatg
    11401 ttttttgcaa actcggcgaa cacttgcgtc gttcttcgcc gaattggaac cgcgccaagc
    11461 gttgcaattt ttccaagcac ctctatggac gcctcaaaat gcatttgcgt tccgcatggt
    11521 gcggcgatca atgaaacttg catcgaaccg gtcatgcggt tgagactcat cgaatcggtt
    11581 cctttgagcg aaactttcaa gagcgaaggc tcaaccgtct cggttatgcg cacctcaaga
    11641 ttcatcgtaa ttttgaacgg gccaattttt tcactcactt tggccgagta cgcttcgttg
    11701 atgtcatcca tcaaacgcgc aacgttttca acacccggca aaagacccgt caagcgcggc
    11761 gtgtcgcgca agagtttcca gacttgatcg atcgacgcgt tgagcggtag ttcttctgtg
    11821 agtttcaaaa gcacaggctg atcttcacca atctttccat ctccgaaatt gcaaaaaaca
    11881 gcgacggaag cggtttgaaa tttccaaaaa caatatcgtt cgctgtcttt tgtaccatat
    11941 ctcccgttgc ctatctgcgg ctatgttgca agcacagaga gcatcttccg tttctcagac
    12001 agaattcagg ggagttgccg tgcaatattg tttttgattc cggcgaggaa cgaatgcgac
    12061 ccaaagcgaa cgtgcggcag ttgcgtttgg aatcgccttg cagggagaat ggcctccatt
    12121 ttattgttgc gggcatggcc cagaccgaag tagtggctat ctgaagataa gcctcgctct
    12181 taatccctgg tttcgtacaa ccatatttca gctccacgat ttgcgggagc gggaacttcc
    12241 caggtagaaa gtgttgcgct ttcctacctg cccggtatac gcgctggtgc gcgcgccgat
    12301 catggtgaga atttttgcta tacgttcgta gcgatttcta tcaatctgtt gatatacgaa
    12361 aatgtaccct ctccactggt gaaaatgcag tacggattgc actcttgaag tcaaagcata
    12421 agctcagtta agctccgccc gatcaggcgc cttgagaaca gaagtcattt ccggaggcag
    12481 catcattcac ttcgtctgtc acaactgtga atttggccag ttcggccata ccaaatgaag
    12541 ttgtaatcgc cacatcggcg gcgtcccgtc cagtcgccat cagaacgcgg ccaatgcgcg
    12601 gttcgttgtg tcgcgcatcg aacgtccacc atcttccttc caaatagact tcaaaccatg
    12661 cgctgaaatc catgggagcg gtcgacgccg ggacgccaat gtcgcctaga tagccggtgg
    12721 cgtagcgcgc ggggatatgc atgcatcggc aaaaagcgat ggccagatgc tggaagtcgc
    12781 ggcagacgcc gacgcgctcg gtataaacat ccagtgccgt cctggtcgag cgcgcatggt
    12841 gatatccaaa ctgcactctg ccgtggatcc agttcacgat agcctgaaca cgtccccagc
    12901 ccggcgcggt atttccaaac aaatccgtcg cgatattaga gaggcgatcg acctcgcagt
    12961 atctgctatt catcaaaaaa gttaagtttt ccgccggcaa atcgatgacg ggatgctgct
    13021 gggcctggca attgatcggg tctttttccg cggaatcttt tatcagagtg gaattccaaa
    13081 tacgcagctt gccttccgga gccagaaatc tggtacagcg gttggcatag cgatcgaaat
    13141 aagtttctgt cttaaccttg ggctcgatac ttaactcgtc cggctcgaga agatctgggg
    13201 cgcgggaagg gtgtacgttc agaagcgcca cgaccgtgac cggatgtgga atattaaatt
    13261 caatgtcgta accgaggcgt atcaacatag gaaggtcgcg tgggcaaagt gtcgagcaaa
    13321 aacacaatcg atatctgcat ctatgagttt acactgcatt cttcatatca gattgaatgt
    13381 cgaaatccat tttttgaatc gtattcatat gcgtccggaa ttctctttcc ggagacgcag
    13441 aaaagccccg cgatattggg tgttcgatga atcctgtttt aatgtgactt ggtcaattac
    13501 ggagctttgc ttaccagttg cccgggcgaa ggaagttgtg atgtgtccca gcctccgccg
    13561 agcgcttcga tcaaaccacc gctcgccgtc atctgctgga tgcgcaaatt cacagccgct
    13621 tcctggtttg agagcaacga agtctgcgcg gtgatcacat tcaagtaggg atcgatgccc
    13681 aactggtagc ggtccgtagc cagggccagg gttctctggg ctgagttgac cgctttatcc
    13741 tggtcctgaa tttccgctga caggattcgc agcgtggaaa gattatcttc cacctgctga
    13801 aacgccacga gaacagcctg acgataattc gccaccgtct cgttgtattg agattggaat
    13861 tgctcaactg ttgcgcgacg caaacccgca tcgaaaagtg attccacgcc cgccgcaccg
    13921 atcgagaaga atcggctctg ccagttgaat agggtagtaa tggaagtgct ttctagaccc
    13981 gcggcgccgg agagtgtgac cgtaggaaag taggcggctc tcgcaattcc aatttgtgcg
    14041 ttggcctgcg ccacgaggcg ctcggcggaa gcgacgtcgg gccgcctttc gagcagttgc
    14101 gatggaacgc cgatgggaat tgctggagga tttgggatga gtggcttcac gggaatagaa
    14161 aatgtcgaag cgggctggcc tgtcagcatg gcaatagcgt gctcgaattg cgcgcgttga
    14221 atgccaaccg ccgtgtcttg agctttcgta gcttccagct gagtttcggc ctgtgccacg
    14281 gcttcttccg cgccgatgcc cgtctggtaa agcgcggtcg tcaagtctag agatttttga
    14341 tacgcgtcaa gagtggagtc gagcagttgc ttaagtgaat cctgtccgcg cagatcaaaa
    14401 taatcgacgg ctacttcggt ttgcatcgta agacgagtgt tctccaaatc cgccgccgta
    14461 gcctgcgccc cgtatgccgc agcacgcacc gtgttgcgaa tgcggccaaa caaatccggt
    14521 tgccaggagg cgtcaaatgg caaggaatat tcggcgaagg taccgctcga agaagaagcg
    14581 gacgcagttc ctgtgctgct tgtggttaca ccctttccaa tcgagggtga ccgttgtaga
    14641 ccgatgctcg gtccaaccga aactgtcgga aaaagttgcg cgcgcgattc tttgaccagg
    14701 gcgcgtgctg caaaaaagtt tgccgccgca gctgcaatcg tctggtttga gacgttgacc
    14761 ttttcctcca gttcattcaa ttcgggatca ttgaaaattt cccaccattt tcctcgaagc
    14821 gcgtcgtcct tcggctgcgc gactttccat ccatccgtat ttttataatc tgccggagtg
    14881 agttccttgt acgccggagc agtggtcgcg gttggcgtgt ggtacttcgg gccgaccatg
    14941 cagccagcca ataaactcga tgtggctagg agaaataccg cggcatgccg gaaaatggga
    15001 gtcataattt ttgtcaactt catattcgcg atggctccat taatctgcgg ggctcggcgc
    15061 tggcccggga tctcttccaa acaccgtttt tcttttgccc tgcatgcgca gtcgtagccg
    15121 gtcgaacgtc agatacacca caggcgtggt gtatagcgtc agcatttggc tcaggatcag
    15181 gccgccaaca atcgtgatgc ccaacggccg gcgaagctca tagcctgttc ccgtgcccat
    15241 cgccagcggc agcgcgccca gcagtgcggc catcgttgtc atcaggatgg gacgaaagcg
    15301 caacatgcac gcctcgaaaa tggaatcgaa cggcgtcttg ccttccagcc gctcttcttg
    15361 aagagcgaaa tcgatcatca tgatggcgtt cttttttacg attccgatca gcagcacaat
    15421 tccaataatc gaaattacgt tcaaatctac cttgaacaga atcagcgcga gcattgcgcc
    15481 aacgctcgca gacggcaaag ttgaaataat tgtcagcgga tgcaccaagc tctcatacaa
    15541 aattcctaga acgatataga ctgccagcag cgccgttaaa accaatacag gctctgtccc
    15601 tagagattgc tggtaagcga gcagcgttcc ggcgaagaaa ccctgcatcg tagagggcgt
    15661 gccaagccgc ctttgcattt gatcaatgct caacgcagcc tggctcagcg aaacattggg
    15721 cgcgaggtta aaagaaagtg tgaccgacgg aaacaaaccc gtgtggttaa ttgcgagagg
    15781 agtggtattc gcctgcgatt tggccacagt gagcagcgga atgctgctct tgctcgacgg
    15841 cgtatgcaga tagatatatt tcaacccttc cgggctttgc caatattgcg gagctacttc
    15901 gagcacaacg taatattgat tcaattgcgt atagatgatg gaaacttctg actgaccaaa
    15961 ggcgctatat agcgacgaat ccagcgactg agccgtgatg ccgagcttcg cggcggtggc
    16021 gcgatcgaag tggagcagtt catcaagccc gccattttgc tgatccgcgc tgacgtcttg
    16081 taaaccgggc agcttcctca tttgcgcgag caggatcggc ccccactttg taaggtcctc
    16141 ttgcgtatcc gattgaagtg tgtattggta catcgcgttg gaggaacgtc cgccgatccg
    16201 tagatcttgc actgcttgca aaaatgcgga agcaacgggc agacgggcca gttgaggacg
    16261 aaggcggttg atcacctgtg aagcgcttgc agtgcgttgc ttaagcggct tgagcgttgc
    16321 atagatgaag cccgtattcg tggctccgtt gccaccggaa aatccgataa cgttattaac
    16381 cgcgggatcg tttttaatca ccgcgccaat ccgttgtatg gaatcgttca tcgcggggaa
    16441 cgaggaatcc tgcggtcctc gcaccgcccc cacaatggcg ccagtatctt cttcggggaa
    16501 aaatcccttg ggaattttga cgattaaaac aacattaagc aggatggtca gcaagagaat
    16561 gacaagggtc ggaatcgggt ttgccaatac ccattccagg ctgctgcgat aaacagaaag
    16621 cacccaatcg aaaaatcttt cgctggccat gtagatgcgc ccatgttttt ccttgcgttc
    16681 gtttttcaat aaatatgcgc acatcatcgg agtggtggtc agcgagatgg ccatggacac
    16741 aacgatagac gtggaaagcg taacggcaaa ttcgcggaag aggcggccaa caattccgcc
    16801 catcaaaagc agcggaataa atacggcaat cagcgaaacg ctgatggtaa aaactgtata
    16861 accgatttcc tgcgcgccct tcagcgcggc ggcaaaaggc gccatcccat tttccaaatg
    16921 gcgcgaaata ttttccatca ccacgatggc gtcatccacc acgaagcccg tagaaatcgt
    16981 catcgccatc agcgaaaggt tgtcaatgct ataaccaaag agatacatca ccgcaaaagt
    17041 gccgaccagc gaaaccggca cggcaatcgc aggaatgagc gtggctcgcc cattgcgcag
    17101 aaaaatgaat acgacgacga tcacaagcgc gatggaaatc atcagcgttc gttcgacgtc
    17161 gcttaccgat gcgcgaatgg ttgtggtgcg gtccaaaacg atagtcgtat cgatccccag
    17221 cggaacagtg gcctgaatgg agggaagctg gttgcgtatg cgatcgacgg tgtcaataat
    17281 gttggcgccc ggttggcgga aaataatgat ggtgaccgca cgctttccgt tcaagtaccc
    17341 gccggtgcgt atgttttgca ctgaatcggt gacttcagct acgtcggaca gtcgaatggc
    17401 ggcgccattt ttgtagccga cgaccagtgg cttataatcc gccgcatgcg aaatctggtc
    17461 attagcaaga atatccacgg ttacatttcc gtcggtaatt tgccctttgg ctaaattgga
    17521 attttgcagg ctgagaactg attgcaggtt cgcgagagtc agtccgtaat tggccaattg
    17581 catcgggttc gcatccacac gtacagaagg gagcgcgccg ccacccaccg tcacttgccc
    17641 caccccctga atttgcgaca gcttctgctg aatcaccgtc gatgcttcgt catacatttt
    17701 gtcagggccg aatttgtccg aggtgagtcc gagaatcatg atcggcgcgt cggccggatt
    17761 cacttttcga tacgtgggat tgccgggcag attcgcggga agataagttc ttgcggcatt
    17821 gatcgcggct tccacgtcac gcgccgcgcc atcgatcttg cggctcaggt caaattgaat
    17881 ggtgatggaa gtagttccca gtgaactcgc cgaggtcatc tccgtcactc cggcaatgtg
    17941 gccgaattga cgctccaacg gagttgcgac ggacgacgcc ataatctcgg cgctcgcgcc
    18001 cggtaaagcc gcaccaacgg aaatggtagg gaagtcaacc tcaggcagcg gagcgacagg
    18061 cagcacgccg taggccacgg ctccggcaag agcgatcgca attgtcagta gtgtggtggc
    18121 taccggcctg cgaatgaatg gttcggaaat attcatgccg gctccggccg ctgtgcatct
    18181 ccggctgttg cattattcgg cttgtgcgaa aacctgcgtc cgagattgtc gaaaaagatg
    18241 tagatgacag gcgtggtgta cagcgtgagc agctggctca gcaacaagcc gccaaccatc
    18301 gcgattccaa gagggcgcct tagctcggaa ccaaagcccc ttccaagtgc gagcggcagt
    18361 cctgccagca aagccgccat cgtcgtcatc atgatgggac gaaaacgcag caggcaggct
    18421 tcgtaaatcg catcggttgc gttctttcct tcgtttcgtt caccctccag cgcgaaatcc
    18481 accatcatga ttccgttctt tttcacgatg ccgatcagca gcacgatgcc gataatggcg
    18541 accacgttca aatcctggtg aaaaaggatc agcgcgagaa acgcaccgac gccggccgaa
    18601 ggcaacgtcg aaagaatcgt aatcgggtgc acaaagcttt cgtacaagac gccgagcaca
    18661 atgtagacgg tgacgagagc ggcaagaatc agcagcggtt cattcgaaag agaattggtg
    18721 aacgatgcag ccgtcccttg aaaacttgct tgtaggcttg caggcatgtg catgttctct
    18781 tgcactttgt tgaccgcggt tatggcgcta cccagcgaag cattcgacgc aagattaaat
    18841 gaaacggtaa tggccggaaa ttgtccctgg tgattgatgg aaagcggttc cgtggtgttt
    18901 tcaaaatgtg tgaacgtgct cagcgggaca gcgttgctca tagctgagct tgttgtcagc
    18961 gcggcgctag tcgtcctgtt ggaggaagtg gaagcgttgg ccgaaagcgc attctgagga
    19021 ggagttaggc tgctcgcaga cggcgtatag agcgcggaag tggtcagcgc gttcgagccc
    19081 gcggaagatg aaccggacga agcgaaggat gtggaagctc ccgtacccga tgctccggaa
    19141 gaagcgttcg cttgaatgaa aagatgattt aacttgttgg gatcgagctg aaattgcggc
    19201 tgcgcttcca ggatcacgtg gtactgattc acctgcgtgt acattgtgtt gatctgccgt
    19261 tgcccaaacg cgtcgtacat cgtgttatcg atcgttgcgg gcgcaatgcc caatcttgac
    19321 gccgtaaccc ggtcgatcac cagattaacg gcgagcccac ccaattgttg atccgtcgcg
    19381 aggtcctcca attccggcaa ttttttcagc tcggtgacga agcgattggt ccagtcattc
    19441 aattcattgg cgtcgggatc ctcaagcgtg tactggtatt gcgtgcggct cacgcgatcg
    19501 tcaaccgtaa tattctgcac aggctccatg aacaattgaa ttccctgaac ctgatttact
    19561 ttggcggtga gcctgcgaat taaattggac gcgcttatgt ttctttgctc aattggtttt
    19621 aggttgatcg acatccttcc gctgtttgtc gttgtgttcg tgccgtccgc gccaataaat
    19681 gacgaaacgc tttccactgc gggatcagcc atcaccactt tcgtaagttc caactgcttt
    19741 tcagccatgg ccttcgagcc gatcacttgc ggagcctgcg aaattccctg aatcactcca
    19801 gtgtcctgaa ccgggaaaaa tccttttgga atgatgatgt acagaaaaat cgtaagcagc
    19861 agcgttgcca ccgccacaag cagcgtaatc gtctgatagc cgagaacaaa tttcagtgtg
    19921 cggccgtaaa actcgatcat cgcctcgaat gcgcgttcgg tggcttgata gatgcggccc
    19981 tgtttttctt ttggctcatg gcgcaagatg cgcgcgctca tcatgggcgt gagcgtgagc
    20041 gatacgacag ccgatacgac aatcgtcacc gcaagcgtca cggcgaattc acggaacaga
    20101 cgtcccacga catcgcccat aaataaaagc ggaattaaaa ctgcgatcag ggaaacagta
    20161 agagaaagaa tggtgaaacc gatttgctcc gcgcccttga gcgcggcttc catcggcgga
    20221 tcgcccgcct cgatgtaccg tgaaatgttt tcaatcatca cgatcgcatc gtccacgacg
    20281 aatcctgtgg aaatggtgag cgccatcagc gataagttgt ccaggctata gcccaggatg
    20341 tacatcgccc caaatgtgcc aaccagtgat agcggcacgg caacgctggg aataatcgtg
    20401 gcgtacagac ttcgcagaaa aaggaaaata accagcacca ccaagcctat cgtcagcatc
    20461 aattcaaatt ccacgtcagc aaccgaagct tcgatcgaag ttgtaaggtc cgtgagcgtc
    20521 gtgacctgta ttcccgttgg caggttcgcc ttcagttgcg gcaaaagatt cttaatactt
    20581 ctgaccacgc tgatcgtgtt cgcccccggt tggcgctgca cgttcaaaat tacggcgggc
    20641 gtctgattca tccacgctgc ctgattattg ttctccaccc cattcacgat gttggctaca
    20701 tcggtaagca tgacgggcgc gccattgcga taggcaacga ctacattctt gtaatcattg
    20761 ctggtgatca gctggtcgtt tgcgtcaatc tgataatcct gccgaactcc gtcgaagttt
    20821 cccttcgccg cattcacgct cgattgcgta agcgccgtgc gcaggtcttc catattaatt
    20881 ccgtaggacg acatggccgt tgggttggcc tgaatgcgga ccgcgggttt ttggccgccg
    20941 ctgatgctta cgagacctac cccgctcaac tgagaaatct tcggcgcgag acgcgtatcc
    21001 accagatcct cgacttgaga aagcggcatg gaatccgagg tgatcgccaa tgttaaaact
    21061 ggcgcatcgg ctggatttgt tttgctgtaa gtaggaggtg ccggaagaat cgacggcaat
    21121 agactttgcg aggcgttaat ggcggactgc acttcctgtt cagccacatc gatcgcaagt
    21181 gaaagattaa attgcagtac gatgaccgaa gtgccgcctg aacttgtcga ggtcatctgg
    21241 cttaagccct gcaactgccc aaactgtcgc tcgagcggag ccgtcacggt ggtggagacc
    21301 acattagggc tcgcaccggg ataaaatgta agaacctgaa tcgtcggata atcaacttct
    21361 ggcaaagcag aaacgggaag ttgtttgtag ctgacaattc caaccagaag aatcgccgcc
    21421 atgagcagcg ctgtggctac cggtcgcaaa ataaatgggc gagatgggct cacggcgcat
    21481 tactcctcgg gagcccgttt ggcgcaggtg tgccggacac aaccacgggc acgttgtcct
    21541 gcaatttgtc aaaactgctg ttggcgacta catccccggg gttgatgccg tctacctgag
    21601 tcattccagc attcgttact cctggtttaa cgctgcgcat atgcgcggaa ttattttgaa
    21661 ttacataaac gaaggaagct tgcccgtttt gctgaatcgc ggaagaggga accagagtca
    21721 cattttgcag cgtg
//
LOCUS       DASZMB010000261         3056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_264634, whole genome shotgun sequence.
ACCESSION   DASZMB010000261 DASZMB010000000
VERSION     DASZMB010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264634"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..542)
                     /locus_tag="VG272_12250"
     CDS             complement(168..542)
                     /locus_tag="VG272_12250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14503.1"
                     /translation="MSTGIGIPNLGAMSQFVGEFDVTSRLTDRTYHCRFSHLWNGVAT
                     RHSDTVDTKFLVDGKGSIVGLAHPGIVEFTARAGRNISDREASYVAAQYLKEQLEQED
                     ERPLYDVSAADVVRIIGLLGIQ"
     gene            complement(563..1774)
                     /locus_tag="VG272_12255"
     CDS             complement(563..1774)
                     /locus_tag="VG272_12255"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HWF14504.1"
                     /translation="MLNPMDEVRPPTRLMLTPGPSCVSPRVYRALAMPLVGHVDPWFT
                     EFMGGVQELLRRVFQTQNNITFPISASGTGGIETAMVNVLEPGDEAIVCVNGIFSERM
                     AEIAERTGAKVHRVLAPLGRTVDLDDVRRAGQGRKIKFVGLAHGETSTGVVTKLDPFR
                     KVADELGALLVVDSVASLAAVPLDVDRQRLDICFSGTQKAISAPPGMAPITVNARVEE
                     MLRSRKSKVQSWYFDLTSVMNYWGKERTYHHTPPVSLIYALHEALRMVLEEGLEASWE
                     RHRQNQAALIAGVEAMGIRPFVENQEDRLPTVTSVWIPAGIDGVKARGRLLNEFNIEI
                     AGGLGEIKTTTWRIGLMGYSSQRANVLLFLDCLERVLLDQGMKLSVGAGIAAAASSFA
                     HAEPVTVGHGK"
     gene            2333..2608
                     /locus_tag="VG272_12260"
     CDS             2333..2608
                     /locus_tag="VG272_12260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14505.1"
                     /translation="MAEKLVLVGVIPEIVVIGPEEQVVWLSDTGNLKIQFDPNRCPFQ
                     SNIFQAPPGCQLQSGPPRPGTNPGSFKYRLWLNDQPVGSGEVILREK"
     gene            complement(2612..3034)
                     /locus_tag="VG272_12265"
     CDS             complement(2612..3034)
                     /locus_tag="VG272_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14506.1"
                     /translation="MQGKSNHRNEYQCSIEVNQNGKYCVRVRASFARKTWVLPVYFLA
                     SNFDRAMRKLEESLQFLQKKEENLWFWGVDSSDDPKFSEELVREAGLKLDRRAEFPGR
                     SSNLTLSPEKPIPAVLLAPIRRGLAHSIDTSRVGMAGN"
BASE COUNT          706 a          904 c          751 g          695 t
ORIGIN      
        1 atcatcgaat ccagcgaaat gccggagcct tccgcggggt ttaacgaatc gctttccgac
       61 gcttaggtgc cgcgcttaca cgcgattcgg aaaagctcta acacagagga cgctgggaaa
      121 ttcttgaaaa ggcgagaacc ccttagcagg ctttggaaac cacccgctta ctgaattcct
      181 agaaggccga taattcgcac aacatccgcc gcggacacgt cataaagcgg acgctcgtct
      241 tcctgctcca gctgctcctt caaatattgc gcggccacat aactcgcttc gcgatccgaa
      301 atattgcgtc cggccctggc cgtgaattca acaattcctg gatgcgcgag ccctacaatc
      361 gagcctttgc cgtcgaccag gaacttggtg tccaccgtat cgctatggcg cgtcgccacg
      421 ccattccaca agtgcgaaaa ccggcagtgg taggttcggt ccgtcagccg ggaggtgacg
      481 tcaaattcac cgacgaattg gctcattgcg cctaaattgg gtattccgat tcctgtgctc
      541 attttctcta gtttcccttt ttctactttc cgtgtcccac agtgacgggc tccgcgtgtg
      601 cgaagctgct tgccgcagcc gcaatgcccg cgcccaccga taacttcatc ccctgatcca
      661 gcaagaccct ttccagacaa tcgaggaaca aaagcacgtt cgcgcgctgg ctggaatatc
      721 ccatcaatcc aatgcgccac gtggtggtct tgatctcgcc aagccccccg gcgatctcga
      781 tattaaattc attcaacagg cggccgcgcg ctttcacgcc atcaatgccc gcgggaatcc
      841 agaccgaggt cacggtcggc aagcggtctt cctgattttc aacaaatggc cgaatcccca
      901 tcgcttccac acctgcaatc agtgcagcct ggttctgccg gtgacgttcc cagcttgctt
      961 ccagaccttc ttccagcacc atccgcaacg cctcatgcaa cgcgtaaatc agcgatacgg
     1021 gcggcgtgtg atgataggtg cgttccttgc cccaataatt catgacgctc gtaagatcaa
     1081 aataccagct ctgcactttc gacttccgcg aacgcagcat ttcctctaca cgggcgttta
     1141 ccgtgatcgg agccatgcct gggggcgcgc tgatcgcttt ctgcgtgccg ctaaagcaga
     1201 tatcgaggcg ttgccgatcc acgtccagcg gcacagctgc aagcgatgcg acgctatcca
     1261 caaccagaag cgcccccaac tcgtcggcca ccttgcggaa agggtcgagc tttgtgacga
     1321 cgccggtcga tgtttccccg tgcgcaagtc ccacaaactt gattttgcgg ccttgtccgg
     1381 cgcggcggac gtcatccaga tccaccgtgc ggcccagcgg cgccaggaca cggtgaacct
     1441 ttgccccggt ccgctccgca atttccgcca tgcgttcgga aaatattcca ttcacgcaga
     1501 cgatggcctc atcgccgggt tccagcacat tgaccatggc ggtctcaatt ccgcccgttc
     1561 ctgatgcgga aatcggaaac gtaatgttat tttgagtctg gaagacacgg cgcaataatt
     1621 cctgcacgcc acccatgaat tcagtaaacc aagggtccac atgtccaacc aagggcatag
     1681 ccaaagcgcg gtacacgcgc ggactgacac acgagggacc gggcgtaagc atcagccttg
     1741 tcggcggtcg cacttcatcc atcggattca acatggaatc aaacctcagc tttcgagtcg
     1801 gatcttgtca ccgttaactt taggaagatt gcacatcaat aatcgaaaat ttagaatact
     1861 cggagcatca aagacggcac aaccttactg ctggctttac tgactacttt cctagccaga
     1921 tggggaagct tctcggaaac aaaatcctga ccactggtat agtttgcgcg attaccagcg
     1981 ctcgcgcaac cgccaaaatg gctgtgtctg agcaatcaga taatcctaag ccactatttc
     2041 cgttcgaatt gctcgcacgg gcaaatttgt tgcgagttca ttccattgct gatttccacg
     2101 ggcaattttt agtttgggtg aaaaaagtcg tccgcccgaa aagcacggcc gttacaatgg
     2161 cggttttgta gcagccactc cggttcgcgt cgttcgggcc ggagtgggcg actttcatcg
     2221 caactggcaa caccacccac aaattttgcg ttgaaccgat ccgttcatat tcttggagcg
     2281 cgctcgtcgc ctgctatgct atcttcgcgt ggcactggag gatggagcat caatggcgga
     2341 aaaattagtt ctggttggcg ttatacctga aatcgtcgta attgggcctg aagagcaagt
     2401 cgtctggctg tccgacaccg gaaatctaaa gattcaattc gacccgaacc gctgtccatt
     2461 ccaatcgaac atctttcaag cgccgccagg gtgccagctg caaagcgggc ctccacggcc
     2521 gggtacgaat cccggctcct tcaagtatcg actatggctg aacgatcagc ccgtgggcag
     2581 cggtgaagtt attctgaggg aaaaataaat ttcaattccc cgccattcca acacggctcg
     2641 tatcgatcga atgcgccaat ccgcgcctta taggtgccaa tagaactgcg ggaatgggct
     2701 tttccggtga aagagtcaga ttggaagacc tgcccggaaa ttcggcccgg cgatccagtt
     2761 ttaggccagc ctcgcggacc aattcttcgg aaaatttggg atcgtcggag gaatccacac
     2821 cccagaacca caaattttct tctttcttct gaaggaattg caacgattcc tcgagcttgc
     2881 gcatggcacg gtcaaagttg gaggcgagaa aataaaccgg taatacccat gtcttgcgcg
     2941 caaacgaagc cctgactcgc acgcagtatt ttccattctg attcacttca atcgagcatt
     3001 ggtactcatt gcggtgatta cttttcccct gcatctggca cctcacgagc taacaa
//
LOCUS       DASZMB010000262         5895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_5212718, whole genome shotgun sequence.
ACCESSION   DASZMB010000262 DASZMB010000000
VERSION     DASZMB010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5212718"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..933)
                     /locus_tag="VG272_12270"
     CDS             complement(<1..933)
                     /locus_tag="VG272_12270"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HWF14507.1"
                     /translation="MEKGTSIRDYDWWLLATALAICGVGVLEIWSATHASHLAGMHTH
                     QLEWIGLGFILMMLLSRLDYHTIMDQAPILYLIAIIALIAVLVIGNSRFGARRWLPIL
                     GGLLQVSELVKLIIIIVLARFFSEVRTEELSLADLIKAAALVGFPVVLIMKQPDLGTA
                     LVLLPLAAVGAYLAGLQWKHALAILALAAVMVPVGYHFLKPYQRERVTTFLRPEDDPK
                     GAGYQILQSKIAVGSGGFWGKGLGNGSQNQLGFVPVRYSDFILAALAEELGFAGVFVA
                     LVLYMFLLLRLVQNAQRAHDRAGMFLIMGVTAVLG"
     gene            complement(941..2800)
                     /gene="mrdA"
                     /locus_tag="VG272_12275"
     CDS             complement(941..2800)
                     /gene="mrdA"
                     /locus_tag="VG272_12275"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HWF14508.1"
                     /translation="MNDKRLPTERLAVVSYVIVAVTVLLLFGFWRLQINDSDRYAQMA
                     ERNRVRSIPIIAPRGSMLDREGRVLADNYPSFSVLLLRDAPDQVEKLLPQIADGLGMT
                     LEEVKQEIDASRAMPKFQPIVIKPEASPSDIAFIESHRGDIPVLEMLMVHRRRYPHGD
                     MLANAMGYVGEVSAEQVEASDSSLKPGDIVGKTGLERQYNSTLMGTDGLRRVIVNSIG
                     KEVGRLEQQDATPGKPIQLTIDYDLQAIADNYMADREGAVVAMDPRTGEILAMVSRPT
                     FDPNDFAIRIPSSEWASLNTDVRTPLLNRAIQGQLAPGSIFKIAMATAMLESHDVPDN
                     FTAFCPGHAQFYGRTFHCWRPAGHGVTDLHKAIVDSCDVFFYTLGQRLGIERIHEYGA
                     GLGLGRRTGVDLPGEEPGLMPSEEWVQRVYHHKWYAGETISVSIGQGAVTVTPIQLAR
                     MVGAVATGGDLPQPHFLKNLAAKMDRFPLSENTVEQVTQGMYGVINEGGTGSSLKLQN
                     IEFSGKSGTAQLMSYEAGNKMGGKKGKETNGWFVGYAPRRNPEIVVAAVVQGSTEHGG
                     TTAGPVVRDVVKAYYDKKNGIKIQEFTTAKAGQPKVVPTSVAGPALAQTQPSR"
     gene            complement(2943..3473)
                     /gene="mreD"
                     /locus_tag="VG272_12280"
     CDS             complement(2943..3473)
                     /gene="mreD"
                     /locus_tag="VG272_12280"
                     /inference="COORDINATES: protein motif:HMM:NF016019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HWF14509.1"
                     /translation="MESTEVYIGPEPHIEVHKYYSGIVPVAVLLALVLQAFLPVYIRS
                     ANYLELPLLVTLYFALSKRNPSSGLILGMVVGLLQDSLSRTPLGLYGIAKTLVGYLGS
                     SLGARIDVEHPIARFLLTVGFYLFHHAIFTLTTRTLLARHEEAYMTLPIAIASLVNAV
                     IAIGLFPFLDRFRKPS"
     gene            complement(3501..4448)
                     /gene="mreC"
                     /locus_tag="VG272_12285"
     CDS             complement(3501..4448)
                     /gene="mreC"
                     /locus_tag="VG272_12285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HWF14510.1"
                     /translation="MIAIPSRHKSLTLLAGVVIVQVLLLAAQIKREQQVRLIRVWAVE
                     LVSPLQRIAAWSINGAEHGWGGYVGLRTAQKENDSMRAELDRLKVRNAELEGRALEAD
                     RLAVLLNFHNAHAETPMIAAHVIGGSPDSGSQTINIDRGSRDGIRRDMGVITPDGVVG
                     KIFAVYPDISQVLLIGDRESGVGALLSDTRTQGPVKGTGEPLLSLDYISNDEKVTVGE
                     AILTSGQDRIFPKDLPVGRVQDFVVDPKTPFMRIRVKPAARLDRLEEVIVLQTRQDLN
                     MKKGPDADTAPIPSPVVPKPAAAPAPPPATPAEKPAGTQ"
     gene            complement(4496..5536)
                     /locus_tag="VG272_12290"
     CDS             complement(4496..5536)
                     /locus_tag="VG272_12290"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWF14511.1"
                     /translation="MSNNGYNLRSVFSLFSSDLAIDLGTANTLVYSKGKGIVVNEPSI
                     VAINKNSGEVVAVGKEAKEMLGRTPGNVVAIKPMKDGVIADFKVTEKMLTYFIQKAHN
                     RRMLVHPRIVIGVPSEITPVEKRAVQDSAYRARASEVYLVEQAMAAAIGAGLPIEEPS
                     GNMVVDIGGGTTDIAVISMSGIVYSRSVRVAGNEMDEAVMQYLKRKYNLLVGERTAEQ
                     IKMEIGSAYPLEKPLTMEVKGRNLIEGVPRTVTIDDSEIREALSECVATILNACRVAL
                     ERTPPELSADISDRGIVLTGGGALLKNLDKRIREETGLPVSIADDPLASVVLGTGKML
                     SDFRLLRLVSID"
     gene            complement(5629..>5895)
                     /locus_tag="VG272_12295"
     CDS             complement(5629..>5895)
                     /locus_tag="VG272_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14512.1"
                     /translation="TLATQGKGQEMLLEVGSTELHGNVVEITGRTEPGAALIINGEQV
                     ANVQNDGQFKYFTSPLTRGSQTIVITGQNRRGGTAIKRVEIVIP"
BASE COUNT         1328 a         1736 c         1530 g         1301 t
ORIGIN      
        1 acctaaaact gccgttacgc ccatgatcaa aaacatgccg gcacgatcat gcgcgcgctg
       61 ggcgttctga accagacgca acagcaggaa catatataaa accagcgcca cgaagacccc
      121 tgcaaaccca agctcttcgg caagcgccgc taaaataaaa tctgagtacc gcacgggcac
      181 gaagcctaat tgattctggc ttccgttgcc cagtccctta ccccaaaatc cgccggaacc
      241 cacggcgatc ttcgactgca ggatctgata gcctgcgccc ttgggatcat cttccggtcg
      301 caggaatgtt gtaacacgtt ctctctggta aggcttcaga aaatgatagc ccaccggaac
      361 catcacagcg gccaaagcca gaatcgccag ggcatgtttc cattgtaaac ctgccaaata
      421 agccccaacg gcggctagcg gcagcaggac gagggcggtt ccaaggtctg gctgtttcat
      481 gatcagtaca acgggaaacc ctacaagtgc cgcggccttg atcaaatccg ccaagctaag
      541 ctcttccgtg cggacctcgg aaaagaaacg agccagcaca atgattataa tcaatttcac
      601 aagctccgac acctgcaaaa ggccgcccag gatgggcagc catcgtcgag ccccaaaccg
      661 cgagttccct atgaccagca cggcgatcaa cgcaatgatc gcgatcaaat acaagatggg
      721 agcctgatcc atgatggtgt ggtagtccag acgggacaag agcatcatca aaatgaaacc
      781 caagccaatc cactccaact ggtgcgtgtg cataccggcg aggtgactcg cgtgcgtagc
      841 ggaccagatt tcaaggacgc caacgccaca gatggccaat gccgttgcca gcagccacca
      901 atcgtaatcg cgaatgctcg tccctttttc cattgaaaat ttagcggctg ggctgtgttt
      961 gcgccagtgc cggtcccgcc acggatgttg gcaccacttt gggttgccct gccttggcgg
     1021 tagtaaactc ctgaattttt attccgttct ttttgtcgta atacgccttg accacatcac
     1081 ggaccacggg accggccgtg gtacctccgt gctccgtact tccctgaaca acggcggcaa
     1141 ccacgatttc cggattgcgg cgtggcgcat aaccaacgaa ccatccgttg gtctccttac
     1201 ctttcttgcc gcccatttta tttccagcct cgtagctcat tagctgggcc gtgccggact
     1261 tgccggaaaa ctcgatattt tggagcttca ggctggatcc tgttccgccc tcgttaatta
     1321 cgccatacat tccctgcgta acctgttcga ccgtattttc ggaaagtgga aaccgatcca
     1381 tctttgcagc cagattcttc aagaagtgcg gctggggaag atctccgccc gtagctacgg
     1441 cgcccaccat tcgcgcaagc tgaatgggag taacggttac ggctccctga ccaatcgaca
     1501 cggaaatcgt ttcgcccgca taccatttat ggtgatagac gcgttggacc cattcttccg
     1561 aaggcatcag ccccggttct tcacccggca aatcgacacc ggtgcggcga cccaaaccca
     1621 ggcccgcgcc atattcatgg atacgttcaa tgcctagcct ctgcccgagc gtatagaaaa
     1681 aaacatcgca ggaatcgacg atcgctttat gcagatcggt gacgccgtgg cctgcagggc
     1741 gccagcagtg gaatgttcgt ccgtagaatt gagcgtgtcc gggacaaaac gcggtgaagt
     1801 tgtccggcac gtcgtgggac tcaagcatcg ccgtggccat ggcaatttta aaaatggagc
     1861 cgggagcgag ctgcccctga atcgcgcggt ttaacagtgg tgttctaaca tccgtgttca
     1921 gagacgccca ttccgatgag ggaatacgaa tcgcgaaatc gttgggatca aatgtggggc
     1981 ggctgaccat cgccaggatt tctccggtgc gcgggtccat ggcgacgacg gcgccttccc
     2041 tgtcagccat gtaattatcc gcaatggcct gcaaatcgta atcaatcgtc aactgaatcg
     2101 gtttgcccgg ggtcgcatcc tgctgctcga gtcgcccaac ttccttgccg atgctgttga
     2161 cgatcacgcg gcgcaagccg tccgtgccca ttagcgtgct gttgtactgg cgttcaagcc
     2221 ctgtcttccc gacgatatct ccaggcttca aactggaatc agaagcttcc acctgttccg
     2281 cgctaacctc tcccacatat cccatggcat ttgcgagcat gtcgccgtga ggataccggc
     2341 ggcggtgaac catcaacatt tccaggacgg ggatatcgcc acgatgggat tcgatgaagg
     2401 caatgtcgga ggggctggct tccggcttga tgacgatggg ttggaatttc ggcatcgctc
     2461 gcgaggcatc aatctcctgc tttacttctt ccagcgtcat gcccaaacca tcggcaattt
     2521 gcgggagcaa tttttctacc tggtcgggag catcacgcaa cagcaaaacg ctaaaggagg
     2581 gataattatc ggcaaggacg cggccttcgc gatccagcat ggaaccgcgc ggtgcgataa
     2641 tgggaatcga ccggacacgg ttgcgctcag ccatctgcgc atagcggtcc gaatcattga
     2701 tctgcagcct ccaaaatcca aacaggagaa ggacagtcac cgctacgatc acataagaaa
     2761 caaccgcgag ccgctccgta ggcagccgtt tatcattcat gaatgaaatg gcggactcga
     2821 ctccgtgcgg ccgttaactc cgcacacctt gcaggttttc tccacactta accatcgatc
     2881 acactagcat ttgcaccgaa cgtgcgcaag atcacacgcg cccaatcgga tacccccttt
     2941 cttcaggatg gctttcggaa gcggtccaaa aaggggaaca aaccgatggc aattacggca
     3001 ttcactaacg aggcaatcgc gatcggcagc gtcatatagg cttcctcgtg gcgtgccagc
     3061 aaagtgcgtg ttgtgagcgt gaaaatcgcg tggtggaaga gataaaaacc gacggtcagc
     3121 aggaatcgcg cgatgggatg ctcaacgtcg atacgcgcgc ccagcgagga gcccagataa
     3181 cctaccagcg ttttcgcaat tccatataaa ccaagcggcg tgcggctcag gctgtcctga
     3241 agcagaccta cgaccatgcc caggattaat ccggacgacg ggttgcgttt gctgagcgca
     3301 aaataaagag tgaccagtaa aggcaattcc agataatttg ccgagcgaat gtaaaccggc
     3361 aaaaatgcct gcaaaacaag cgccagcagc acagcgacag ggacgattcc gctatagtat
     3421 ttatgaactt cgatgtgggg ctctggccct atgtagactt cagtactttc catattttct
     3481 gcccatattt actatttggt ttactgtgtc cccgctggtt tttccgctgg cgtcgctggc
     3541 ggaggcgcag gcgcggccgc gggtttcggt acaacaggag atggaatggg ggccgtgtcc
     3601 gcatccgggc cttttttcat attcaaatcc tgcctggttt gcaggacgat gacttcttcc
     3661 aaacgatcca agcgagcggc gggttttaca cgaatccgca tgaacggcgt ttttggatcc
     3721 acaacaaaat cctggactct gcccacgggc aaatcctttg ggaaaatacg gtcttgaccg
     3781 gaagtcaaaa tcgcctctcc cacggtaact ttttcatcat tactaatata atcaagggaa
     3841 agcagcggtt cgcccgttcc cttcaccggc ccctgcgttc gcgtgtccga gagcagagcg
     3901 ccgacgccgc tctccctgtc gccaatcagc agtacctgcg agatgtccgg atacacggca
     3961 aaaattttcc ctacgacgcc gtccggcgtg atcacgccca tgtcgcgtcg aataccatcg
     4021 cgcgatccgc ggtcaatatt tatcgtctgg cttcccgagt ccgggcttcc gccaatcacg
     4081 tgcgcggcga tcattggagt ttcggcgtgg gcattatgaa agttgagaag aacggcgagg
     4141 cggtccgcct ccagagcacg gccttcgagt tcggcgtttc gcactttcaa gcgatcgagt
     4201 tccgcgcgca tggaatcatt ttctttttgc gcggtgcgca ggccgacgta tcctccccaa
     4261 ccgtgctcgg ctccattgat ggaccaggcc gcaatccgct gcaagggaga aaccagttcc
     4321 acggcccata cgcggataag ccgcacctgc tgctcgcgct tgatctgcgc ggccagcagc
     4381 aaaacttgta cgatgacaac gcctgcaaga agcgtcagag atttatgccg agaaggaatt
     4441 gcaatcatcg cggaaacagc cagctttcaa aattttttgc accggacatg caaaattagt
     4501 cgatggagac gaggcgcagc agccggaaat cgctcaacat tttgcctgtt ccgagcacca
     4561 cggacgccag cggatcatcg gcaatggaaa ccggcaaacc ggtttcttcg cgaatgcgct
     4621 tgtccaaatt cttcagcaac gcgcctccac cggtcagcac gataccgcgg tcactgatgt
     4681 cggcggaaag ttcgggcggc gtgcgttcga gagccacgcg gcaagcgttc aaaatggttg
     4741 cgacgcattc ggagagcgct tcgcggattt cgctgtcatc aatcgtaacc gttcgcggca
     4801 cgccttcaat caaattgcgg cccttcactt ccatggtcag cggcttttcc agcggatacg
     4861 ccgatccaat ttccatttta atttgctcgg ccgtgcgctc gccaaccagc aagttgtatt
     4921 tgcgcttcaa gtactgcatc acggcttcgt ccatttcatt tcctgccacg cgaaccgaac
     4981 gcgagtacac gatgccgctc atggagatta cggcgatatc ggtcgtgcct ccgccaatgt
     5041 ccaccaccat gttccccgaa ggctcttcaa tcgggaggcc cgcgccaatc gccgcggcca
     5101 tggcctgctc cacaagataa acctcgctgg cacgcgcacg gtaggcggag tcctgcaccg
     5161 cgcgtttttc caccggcgtg atctcgctgg gcactccgat gacgatgcgc ggatgcacga
     5221 gcatgcggcg gttgtgggct ttctgaatga agtaggtgag catcttttca gtcaccttga
     5281 agtcggcgat gacgccatcc ttcatcggct tgatggccac tacgttgccc ggcgtgcggc
     5341 caagcatttc cttggcttct ttgccaaccg ccacaacttc tccgctgttt ttgttgatcg
     5401 ccacgatgga cggctcgttc accacgatgc ctttaccctt ggagtaaacc agcgtgttgg
     5461 ccgtccctag atcgatcgcc aagtctgaag aaaacagact gaagaccgat cggagattgt
     5521 aaccattatt cgacatgttt ccctcagtgc cgggccgctc gcgcggctgc tggatgccgc
     5581 atgcttgggc gtccccggct gcccttctct aaaatttttc ccgacgggct atggaattac
     5641 tatttccact cgcttgatcg ccgtgccgcc tcgcctattt tgtccggtga tgacaatcgt
     5701 ttggctgcca cgcgttaaag gcgacgtaaa atatttaaac tggccgtcgt tttggacatt
     5761 tgctacctgt tcaccattaa taataagtgc cgcgccaggt tccgtgcgtc cggtgatctc
     5821 taccacgtta ccatgcaact cagtgcttcc gacctccagc aacatctcct gtcccttgcc
     5881 ctgtgtcgcg agtgt
//
LOCUS       DASZMB010000263         2916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_1902581, whole genome shotgun sequence.
ACCESSION   DASZMB010000263 DASZMB010000000
VERSION     DASZMB010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1902581"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VG272_12300"
     CDS             <1..435
                     /locus_tag="VG272_12300"
                     /inference="COORDINATES: protein motif:HMM:NF014804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase"
                     /protein_id="HWF14513.1"
                     /translation="VDLIKSQIRLATGEKLSEVVTNFALRGHAIECRINAEDPETFVP
                     SAGHITVFRTPGGPGIRVDTAAHADAVIPPYYDSLVAKLIAFGDTRTEAIVRMRRALE
                     MLVVEGIKTSIPLQRKIMMDPDFSAGKLDTHFLDRFATSHAG"
     gene            442..1083
                     /gene="thiE"
                     /locus_tag="VG272_12305"
     CDS             442..1083
                     /gene="thiE"
                     /locus_tag="VG272_12305"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HWF14514.1"
                     /translation="MNLVFPPLYAIIDPSLLTISELAMAEALAESGVQLIQYRNKTAS
                     SRQFFEISRKLSSALAPIHVRLIVNDRPDVALLSGAGGVHMGQDDFGVEDARKICGPN
                     CWVGISTHNLEQLAAANQTSADYIAVGPIFPTATKKNPDPVVGTELLRKAREMTKKPL
                     VAIGGITLERAAEVYRAGADSLAVIRDLICTTNPGERAREYLNVAASSAGRAI"
     gene            1143..2720
                     /locus_tag="VG272_12310"
     CDS             1143..2720
                     /locus_tag="VG272_12310"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HWF14515.1"
                     /translation="MKEERPVPEVKSNVTTVAAEADSSGFVRGLGLFDSTTIVVGSMI
                     GSGIFIVSADIGRNTGSTGGLLLAWIVAGLMTVTAALSYGELAAMMPKAGGQYVYLRE
                     AYSPLWGFLYGWTLFLVIQTGTIAAVAVGFARFLGVIWPTISPKTWIVAPIALSDKYA
                     ISLSLQQLVALLLILFLTALNMRGLKLGKWIQNIFTSAKTLSLAALILAGIFLGRNAA
                     AIHANFTGIWTLHDPQTIEPGANWMKSFIPSVTAASGTFGLLVAFGVSQVGSLFSADA
                     WNNITFTAGEVKNPKRDIPVSLAVGTVVVITLYFLANIGYLSTLTLHQIQTAPDDRVA
                     TATLAAIFGNSGATLMALGIMISTFGCANGLILAGARVYYAMSRDGLFFRGIGKLNAQ
                     HVPAMGLVLQGIWAAILVLLRTRLVNATTGVVSFGNLYGDLLDYVVFAVLIFYVLTIG
                     GLFVLRRKRPDAERPYRTWGYPIVPILYIVTACIIMLILILYQTQDAWPGLVIVMLGV
                     PVYWAWTRRSGTENSRL"
BASE COUNT          595 a          774 c          840 g          707 t
ORIGIN      
        1 gtggatctga ttaaatcgca gattcgcctg gcaaccggcg aaaagctgag cgaagtggtc
       61 actaattttg cgctgcgcgg gcacgcgatt gaatgccgaa tcaatgccga agaccccgaa
      121 acatttgttc cttcggctgg acacatcacc gtatttcgca cgccgggcgg tcctggaatt
      181 cgagtggaca cggcggcgca tgccgatgcg gttattcctc cctattacga ttcgctggtg
      241 gccaaactga tcgcgtttgg cgacactcgc acggaggcca ttgtgcgcat gcggcgggcg
      301 cttgaaatgc tcgtggtcga gggcatcaaa acgtccatcc ccctgcaacg caaaatcatg
      361 atggatcctg attttagcgc ggggaaactg gacacgcact ttcttgaccg attcgccaca
      421 tcgcacgccg gataattcca tatgaacctt gtgttccctc cgctctatgc aataatcgac
      481 cccagtttgc ttacgatttc ggagcttgcg atggccgaag ctctggcgga atccggggtc
      541 cagctgattc agtaccgcaa taaaacggct tcttctcgcc aattttttga aatttcgcgc
      601 aagctctcga gtgctcttgc tccgatccat gtaaggctga ttgtaaatga ccggccggac
      661 gtcgcattgc tgtcaggcgc gggcggcgtg cacatggggc aggatgattt tggcgtggag
      721 gacgcgcgca aaatctgcgg gccgaattgt tgggtgggaa tttccacgca caatctggag
      781 caactggccg ccgccaatca gacttcggcg gattacatcg ccgtgggccc aatttttcca
      841 actgcaacca agaaaaatcc ggaccctgtc gttgggacag agctattgcg taaggcacgg
      901 gaaatgacaa aaaagcctct ggttgcgatc ggcggaatta cactggaacg tgccgcggaa
      961 gtgtatcgtg caggcgcgga ctcgctggcc gtgattcggg atttgatctg caccacaaat
     1021 cccggtgagc gtgcccgcga gtatctcaat gtagcggcat ccagcgcagg ccgggcaatt
     1081 taatcaatcg aggaacctgc tttttgcttg ggacattttg cgggcagcgt tcagcccgcc
     1141 gaatgaaaga ggaacggccg gtgcccgaag tgaaatccaa cgtaacaacg gttgccgctg
     1201 aagcggattc ctcgggattc gtgcgaggct tgggcttgtt cgattcgacg acgattgttg
     1261 tcgggtcaat gatcggctcg ggaatcttca tagtttccgc ggatatcggg cgcaatacgg
     1321 gatcgacggg cggactgctt cttgcctgga tcgttgccgg cctaatgacg gtaacggcgg
     1381 cgctttctta cggggagctc gcggccatga tgcccaaggc cggcgggcaa tatgtctatc
     1441 ttcgcgaagc atactcaccg ctctggggat ttttgtatgg ctggacactt ttcctggtta
     1501 ttcaaaccgg cacgattgca gcggtagccg tgggttttgc acggtttctc ggcgtaattt
     1561 ggccgacgat ttccccaaaa acctggatcg tcgctcccat cgctctctcg gataaatatg
     1621 cgatcagcct atccttgcag caacttgtgg cgctcttact gattttattt ttgacggcgc
     1681 tgaatatgcg cgggttgaag cttggaaaat ggattcaaaa tatttttact tccgcgaaga
     1741 ctctgtcgct cgctgcgctg atcctggctg gaatttttct gggacgaaat gccgccgcaa
     1801 ttcatgcaaa tttcactggc atctggacgc ttcacgatcc tcagacgatt gagccgggcg
     1861 ccaattggat gaagtcgttc attccatccg taacggctgc ctcaggaacg ttcggtctgc
     1921 tcgtcgcgtt tggcgtttcg caggtgggct cacttttttc cgcggacgcg tggaacaaca
     1981 tcacctttac cgcgggcgag gtaaaaaatc cgaagcgcga cattcctgtc tcgctggctg
     2041 tgggcaccgt ggtggtcatc acgctctatt ttctggccaa cattggatac ctttcgacgt
     2101 tgacgctgca ccagattcag acggcgccgg acgatcgcgt cgccaccgcc acgctggccg
     2161 cgatttttgg aaatagcggc gcgacgctga tggcgttggg gatcatgatt tccacgttcg
     2221 gatgcgcgaa tggactgatc ctggccggcg cgcgcgttta ctacgccatg tcacgcgacg
     2281 gattattttt tcgcggaatt ggaaagctga acgcgcaaca tgttccagcc atgggtctgg
     2341 tgctgcaggg aatatgggcc gcgattctgg tgttgctcag gacgcgcctc gtgaacgcga
     2401 ccaccggcgt ggtgagcttt ggaaatcttt acggcgattt gttggactat gttgttttcg
     2461 cggtgcttat tttttacgtg ctcacaatcg gcggcttatt tgttctgcgc cgcaaacgcc
     2521 cggatgccga gcgaccatac cgcacatggg gatatcccat cgtgccgatt ctttacattg
     2581 tgaccgcatg cattatcatg ctcattttga tcctgtatca gacgcaggat gcgtggccag
     2641 gcctcgtgat cgtgatgctc ggagtacccg tgtattgggc atggacgcga cgttccggaa
     2701 cagaaaactc ccgcctctga cccttcgcat tctctcaggg caagcgaggc gggctgctgt
     2761 agcccgggtc tccagacccg ggatttgctg agtgtgaggc gcgacttaac cggattcatg
     2821 cagcagtgac ctgaactatc aaccggtaaa aggtggatat ggccaaccaa ttatttcaag
     2881 tcaagacgca cgaggcgctg gtcgaggaac tgcagg
//
LOCUS       DASZMB010000264         3923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3570369, whole genome shotgun sequence.
ACCESSION   DASZMB010000264 DASZMB010000000
VERSION     DASZMB010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3570369"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..349)
                     /locus_tag="VG272_12315"
     CDS             complement(125..349)
                     /locus_tag="VG272_12315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14516.1"
                     /translation="MKLTSRTIFIVSGDLATAERCVEDLTAYGGHYRIPVVRTVEQAR
                     KSMGRIAPAAVFLDESAIDTVNGNERELYR"
     gene            complement(357..2528)
                     /locus_tag="VG272_12320"
     CDS             complement(357..2528)
                     /locus_tag="VG272_12320"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWF14517.1"
                     /translation="MNRIVAVPVDVLEKPRAVLMAGFKQTENTDSDVSTVEDYAMAIS
                     AALDRNVIREEQTASEKILLKMLENSTECLLMIDAQGKILESSRAATSQLFTPSGRME
                     GMLLEELFFPLARKEVIAWRTHIRLMDEALKEKKQTVAYVLQGTLDRGGVVRMTLRCE
                     IAGHHGKEARWVIHVEQVKNRQVSHEAAERLQVEMASLLASIESGVLIFDADGRILMA
                     SERLAAIFGMESRHILEFGTIYALIDNLSYYFACPAETAIRWREHILQSEQASWDEIE
                     LVHPSPRMVERFARPLVMPDGERLGWLEVYRDITGQRMVQSKMLQTEKMAALGQLVSG
                     IAHELNNPLTSIQGYAQLLLSRRTGSERVADAKRISQEAERAGRIVKNLLLFSRETKA
                     ERRAVQLNDVVERTLSLRAYELKLQNIEVNLSLDPDLPRTLADAAQLQQAVLNLIVNA
                     EQAIAMGRADETAVGHILIRTRHLPGDRLQLEVSDDGPGIPPEIVSRIFDLFFTTKPP
                     GIGTGLGLSIVYGIVQEHGGDVIADNRKGRGATLSLELPAISVSEFSFASEELTGPSH
                     SQVEPGLPLRGRTGAKKHILIVEDEPTVVELIAEVMADEGHRVDMLLDSRAALGKLDE
                     NDYSLVICDLKMPYVDGPSLYRALVRKANPMQHKLLFVTGDTMGTRTIEFLKSSGLPY
                     LAKPFLVEELKEAVHKALAAVQGIEVLTSESGHSRAVGKNR"
     gene            complement(2557..3321)
                     /locus_tag="VG272_12325"
     CDS             complement(2557..3321)
                     /locus_tag="VG272_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14518.1"
                     /translation="MHGLPRLNGEPFVQMLHLGARAMLVASGADRAGLWLSGGQRGES
                     GPGCAIHSDASPIPEQWNALDISAPFLRAALESSSPLRVDLVADEVTPQLGPLTGMHS
                     VIWVPLRAGHHTVGLGMVGYTHSPEVLNLNLDALRTLAEEIAISVQCPFPSAVKRRGS
                     EDAKSQLRLFRCLQADAAGDSAFRQIARTVRNFSRADFIAMGDGVIPPLAQEGWNGPP
                     DLLPLLRDERVLQMWHKAQGEGRKAEISGEIAAKIS"
BASE COUNT          868 a         1139 c          963 g          953 t
ORIGIN      
        1 aggcccggca cttgtggtga tttgtatcac tttcgggttt gccctagcgg tattgggaca
       61 tttctttcag ttaaatgccc ttgcggagca aaagaagatt taaacggaga cgatttttag
      121 ggagtcatct atatagttcc ctttcatttc cgttgacagt atcaatcgcg gattcgtcga
      181 gaaacaccgc tgccggggca attcgcccca tgctcttgcg tgcctgctcc actgtcctca
      241 caactggaat gcggtagtgg cctccatagg cagtgaggtc ttccacgcat cgctccgctg
      301 ttgctaaatc gccgctgaca ataaaaatag tacggctcgt caatttcatt tcgtcctcat
      361 cgattctttc caacagcgcg tgagtgcccg ctctcagaag tcaaaacttc aatcccttga
      421 acggctgcaa gcgctttgtg cacggcttct ttcaactcct ccaccagaaa aggttttgcc
      481 aggtatggca agccgctgga cttcaggaat tcaatggtgc gcgtgcccat cgtgtctccg
      541 gtcacaaaca gaagtttgtg ctgcatggga ttcgcctttc ggaccagcgc gcgataaagg
      601 ctcgggccat ccacatacgg catttttaaa tcgcagatca caagcgagta atcgttttca
      661 tccaacttgc cgagcgccgc gcggctgtcc aacagcatgt ccacgcgatg gccttcgtcg
      721 gccatcactt cggcaattaa ttccacaacc gtaggttcgt cttccacaat caaaatatgt
      781 ttttttgcac ccgtacgtcc ccggagcgga aggccaggtt cgacctgcga gtgcgatgga
      841 ccggtgagtt cttcgctcgc gaaactaaat tcagatacag agatggcagg cagttcaagc
      901 gaaagagttg cgccacgtcc cttgcggtta tccgcgatga cgtccccgcc gtgctcctgc
      961 acgattccat acacgatgga aagtccaagg ccggtcccaa tgccaggggg cttggtcgta
     1021 aaaaagagat cgaatatgcg cgagacaatt tcaggcggga ttcccggtcc atcgtcgctc
     1081 acctccagtt gcaggcgatc tcccggtaga tggcgcgtgc gaatcaggat gtgtcctacg
     1141 gcagtttcgt ccgcgcgacc catggcaatg gcttgttccg cattcacaat cagattcagc
     1201 accgcctgct gcaattgcgc cgcgtccgcc aatgttcgcg gcaggtcggg atccagtgac
     1261 aaattcactt caatattttg cagttttaat tcgtatgcgc gcagggaaag cgttcgctcg
     1321 accacatcgt tcaactgcac cgcgcgacgc tctgccttgg tttcccggga gaaaagtaaa
     1381 agatttttta cgattcgccc ggcgcgttcg gcttcctgcg agattcgctt ggcgtccgcc
     1441 acgcgctcgg acccggttct ccggctcagc aaaagttgcg cgtagccttg tatgctggtc
     1501 agcgggttat tcaactcgtg cgcaattccc gacacgagtt gtccaagcgc cgccattttt
     1561 tcagtctgca gcattttgga ctggaccatc cgttgcccgg taatgtcgcg gtaaacttca
     1621 agccacccga gccgttcacc atccggcatg accaatggcc gcgcaaagcg ttccaccatt
     1681 cttggtgagg gatgtaccaa ctcgatttca tcccagctgg cttgttcgct ttgaagaata
     1741 tgttctcgcc agcgaattgc ggtttccgct ggacacgcaa aatagtagga aagattgtcg
     1801 atcaacgcgt aaatcgtgcc aaattccaga atgtgccgcg attccattcc aaaaattgcc
     1861 gccaaccgct cgctcgccat aagaatgcgc ccatccgcgt caaatatcag tacgccggat
     1921 tcgatggatg cgaggaggct ggccatttca acttgcagcc tttctgctgc ttcgtgcgaa
     1981 acctgacggt ttttaacctg ctcaacgtga atcacccacc gcgcctcttt cccgtgatgt
     2041 ccagcgattt cgcagcgcag cgtcatgcgc acgacgccgc cccgatcgag agtgccttgc
     2101 aacacgtacg ccacagtttg cttcttctct ttgagcgctt cgtccatcaa gcggatatga
     2161 gtccgccatg cgataacctc tttgcgcgcc aatggaaaaa ataattcctc caggagcatt
     2221 ccctccatcc ggcctgatgg tgtaaacaat tgcgacgtcg cggctcgact cgattccagg
     2281 atttttccct gggcatcgat catcaggaga cattccgtgc tgttttctaa cattttgagc
     2341 aggatttttt cggaggccgt ttgctcctcc cggataacat ttcgatccaa ggccgcggag
     2401 atcgccatgg cgtaatcttc cacggtcgaa acatcgctgt cggtattttc agtttgttta
     2461 aatcccgcca ttaaaacggc gcgcggcttt tccagcacat caaccggaac ggcgacgatg
     2521 cgattcaaac tgcgctgttc aaaacctttc aaatccctaa gaaatcttgg cggcaatctc
     2581 tcccgagatt tcagccttcc tgccttctcc ctgagccttg tgccacattt gtaaaactct
     2641 ttcgtcccgt aaaaggggga gcaaatccgg cgggccgttc cagccctctt gcgcaagagg
     2701 aggtataacc ccgtctccca tagcaataaa atccgctcgc gaaaaattcc ggaccgtccg
     2761 ggctatctgg cgaaacgcgg aatcgccagc cgcgtcggcc tgcaggcatc gaaaaagccg
     2821 caattggctt ttcgcatctt cgcttccacg gcgcttgacg gcggaaggaa atggacattg
     2881 gacggatatg gcaatttctt cggccagtgt cctcagtgcg tccagattta ggtttaatac
     2941 ctcaggagaa tgcgtatagc ccaccatgcc cagccctacc gtatgatgac ccgcgcgaag
     3001 aggcacccaa atcacgctgt gcatgccagt caatgggcca agttgaggcg ttacttcgtc
     3061 ggctaccaaa tccacacgca gcgggctgga actctcgagc gcagccctca aaaagggagc
     3121 cgaaatatcc agcgcgttcc attgctcggg aatcggactt gcatccgagt gtatcgcgca
     3181 tcccggaccg gactctcccc tctgccctcc ggaaagccat agccccgctc gatccgcacc
     3241 gcttgccacc agcatcgcgc gagcgcccag gtgaagcatt tgaacgaaag gctcgccgtt
     3301 cagacgagga agcccgtgca tattcgcccg atcctccgct cggcgtgccc tccgccatct
     3361 tcgttcccaa ctggctgctg gcatttgcct ttacacccgc gccagaagaa ttaaatgacc
     3421 gagcaacatt gctgctccgg tggtgatcgc ggctgcggtt acctctcgaa tcgcccggta
     3481 gtgcgagaaa taaatgtgac ggatcatgac ttcctcctat cacttgtttg tcttgcacca
     3541 cggtggccaa taggaaagag gcgataactt attcagaatc aattaattgc ggatgcctgg
     3601 aaaagttgag ccaaggtttc gtattcccat cccgaaactt ctccgcgaat tgcttcccgg
     3661 agtggaaaat ttttgatatt ggcaatcact tctttcattc agcttccaat gaatccaggg
     3721 cttgctctgg ctgttttttc cagaaaaaaa cattgcgttt cgcccggcct tgggctgact
     3781 tactaactga gaaatagttg atctcagtag aagtcttact gaacgacttc atttagcgtt
     3841 ccatgcgact ggcgttccag ccattaccgc ggatcacgtc taacgcgcaa agtgtgacac
     3901 agataatttc ctcgaagagg cga
//
LOCUS       DASZMB010000265         6367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_2747399, whole genome shotgun sequence.
ACCESSION   DASZMB010000265 DASZMB010000000
VERSION     DASZMB010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6367
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2747399"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VG272_12330"
     CDS             complement(<1..565)
                     /locus_tag="VG272_12330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14519.1"
                     /translation="MTLRGKTLSAIASELPLQILLLGAGVFLLVIPFLPERLAFAQPI
                     PQDAPGQTKIAEGEYAIHERHNGGAVGPFEEEVFDFRESWTLSRVASGEYKIEGVRRF
                     ESPKDESHSNRFTVDLTRDLTVIRAKEFAKLKWVRNSGPLSCNFLPTELQCSSGGSDP
                     KHEIKLRTPLNRPYALLWPVSPFSFGGI"
     gene            complement(618..1067)
                     /locus_tag="VG272_12335"
     CDS             complement(618..1067)
                     /locus_tag="VG272_12335"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWF14520.1"
                     /translation="MKMLIAIDGSDFSEAALQSVIARPWNAGTEIKVLHVVEPPALLI
                     GREMGAYDPEFEMVWKAVRERAKDLMDKAAHKLRAAGFQVSTELVEGEPKSLIIDAAN
                     EWHADMIVVGSHGWSGLNRFLMGSVSEAVVRHAHCSVEIIRTPSVNN"
     gene            complement(1144..2343)
                     /locus_tag="VG272_12340"
     CDS             complement(1144..2343)
                     /locus_tag="VG272_12340"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HWF14521.1"
                     /translation="MKRSKDRILTTHVGSLIRPSQLLDRVSAREKGGPADIKAYEQCL
                     KESVAEVVKRQVQAGINVVNDGEFGKSTSWALYALKRVSGFEMRPVAAGANPFARGAD
                     RERFKEFYAELEGGNDRTWSNVTQQEAVCVGPVKYTGLTELQNDIDNLKAALAGVSVE
                     EAFLPVAAPSSAIPDRKNEYYKNEEELVVALAEALRTEYRTIVDAGFLLQVDDARAAV
                     TYDRMVPPASFEDYYKWVGRHVEVLNHALEGIPEDRIRYHVCWGSWPGPHTTDVPLRK
                     IVDLILKVRAGAYLIEAANPRHEHEWRVWKDVKLPTGKILAPGVVSHGTNVVEHPELV
                     AERITRFANLVGRENVIASTDCGFSQEQFNRRVHPSIMWAKLEAMAEGARIASEELWS
                     HAGSARA"
     gene            complement(2355..2930)
                     /locus_tag="VG272_12345"
     CDS             complement(2355..2930)
                     /locus_tag="VG272_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14522.1"
                     /translation="MTLLLVATIFLQAALGAPLVDNEFVRVFKNSAPCAAAAASCGER
                     VVVALGPLELNGQKMERGDIKGFKAGEHYAAPKAGNFLEISIKPTHPNVMPPVAGTPA
                     APHNKVLYDAQDITVFEEKMQPGEYDPQHSHNVRLAIFLNDTMVEQWTDGKDETRELI
                     PDVVTWRPAVVHASKDVGKVPIHNLLIEFKP"
     gene            complement(3106..4527)
                     /locus_tag="VG272_12350"
     CDS             complement(3106..4527)
                     /locus_tag="VG272_12350"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HWF14523.1"
                     /translation="MSDYPKIIQGGMGAGVSNWRLAQAVSKLGHLGVVSGTALDQILA
                     RRLQDGDPGGHMRRAMAHFPARKMAERIELEYFLPGGKSERTPYKPMPMHDKDGPRDR
                     EELCVVANFVEVFLAREGHDNPVGINYLEKIQIPHLPSIYGAMLAGVGYVLMGAGIPV
                     KIPGVLDRFVHHEAATYPLHATGSQEGQEAEMSFDPKSFVPGELPPLTRPRFLAIIAS
                     NVLAAAMLKKASGSVEGFVIEGPTAGGHNAPPRGKLQLDENGDVIYGERDVVDLEKIR
                     QLGKPFWLAGGYGSAEKLREALSVGATGIQVGTAFAFCEESGLREDYKRAVLEKVSHS
                     EIRVTTDPTASPTSFPFKVAQLDGTLSEEEIYLERPRICDLGYLRETYRTPEGQIDYR
                     CAGEPVSVYVSKGGKAEDTAGKRCLCNALLATIGHPQVRNGNLTEPGVVTSGNDLATL
                     GRFLPADKPSYEAADVIAKLLTV"
     gene            complement(4776..5258)
                     /locus_tag="VG272_12355"
     CDS             complement(4776..5258)
                     /locus_tag="VG272_12355"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWF14524.1"
                     /translation="MSSEREREARQRFQDAFAAQMAGELDRAIELYLGSIELFPTAEA
                     YTFLGWTYHFQGKIEEAIAECKRAIQIDPDFGNPYNDIGVYLIDLNRHDEAIPWLEQA
                     MKAKRYDPRHFPYYNLGRVYLHKGLINRARELFQESLNIEPRYALARAGLESARRMLN
                     "
     gene            5523..6233
                     /locus_tag="VG272_12360"
     CDS             5523..6233
                     /locus_tag="VG272_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14525.1"
                     /translation="MPVCKLCERDFTQEEIDNGVTLPHTTASRIRLVRFSNGTLHQFE
                     KKSNVQLPHGTYVTAKPTPVEAPLAEVEALTDPVEQPPQAIETPDAGQQPDVESLAHD
                     LDAHFHPEPELPEAKNIGLNKAEADDGYVDCFVVFMTSHDTQGRGRGPIGIARKVNRR
                     RDNHDGFRFRVADVVTTGGETIKPGSKIRGRVVADSEYERLIDIEVYIPQEKSKRPPS
                     PVKKREPAIKIYKHDEEE"
BASE COUNT         1450 a         1846 c         1645 g         1426 t
ORIGIN      
        1 taattcctcc gaaggaaaat ggcgagactg gccagagaag cgcataaggt ctgttcagag
       61 gcgttcgcag tttaatttcg tgcttaggat cagatccccc cgacgagcac tgcagttccg
      121 tcggcaaaaa attgcatgac aacggtccgg agttcctcac ccacttcaat ttcgcaaatt
      181 ccttggcgcg aatcaccgtt agatcgcgag ttaggtcaac tgtaaagcga ttcgagtgag
      241 attcatcctt gggagattca aagcgcctga cgccctcgat tttgtattcg ccacttgcga
      301 cacgcgaaag cgtccaggat tcgcggaagt cgaaaacctc ttcctcaaac ggaccgacgg
      361 cgccgccatt gtggcgctca tgaatggcat actcgccttc cgcaattttg gtctgccccg
      421 gcgcgtcctg gggaatcggc tgcgcaaatg ccaatcgctc cggaaggaac ggtataacca
      481 gcaaaaaaac accggcgccc agaagtaaaa tttgcaacgg aagctcagat gcgattgccg
      541 agagcgtttt accgcggaga gtcacatggt tccccaagtt agattcgcga gcgacacaca
      601 ggagcacttt ccgaaagtta attgttgacc gaaggtgtcc gaataatttc caccgagcaa
      661 tgtgcgtgcc gcaccacagc ttccgaaacg ctgcccatca ggaacctatt taatcctgac
      721 catccgtgtg agcccaccac aatcatgtct gcgtgccatt cattggccgc gtcgatgatt
      781 aacgacttcg gctcgccctc gaccagttcc gtggacacct gaaatccagc cgcgcgcaat
      841 ttgtgcgcgg ctttgtccat cagatccttg gcccgttccc gaaccgcctt ccagaccatt
      901 tcaaattccg gatcgtacgc gcccatctcg cggccgatca acaacgcagg cggttcgacc
      961 acgtgcagca cttttatttc ggttcccgcg ttccacggcc tcgcgatcac gctctgcaga
     1021 gccgcttcag aaaaatcaga tccgtcgatc gcaatcagca ttttcatttt acggccagtc
     1081 ctttatccat aatttggcga tacttgctgt accgcgccgc tctctttcat tcaagctatt
     1141 cgtttacgcg cgcgccgaac ccgcatgact ccaaagctct tcgctcgcga tgcgcgctcc
     1201 ctcggccatc gcttccagct tggcccacat gatcgatgga tgcacgcgcc ggttaaactg
     1261 ctcctgcgaa aagccgcaat ccgtgctggc aatcacgttt tcgcgtccca ccagattcgc
     1321 gaagcgcgtg atgcgctccg caacaagctc agggtgctcc accacattcg tcccgtggct
     1381 caccacgccc ggcgccagaa tttttccggt cggcagcttc acatctttcc acacgcgcca
     1441 ttcatgttca tggcgtggat tagccgcttc gatcagatac gctcccgcgc gaactttcaa
     1501 aattaaatca acaatttttc gcagcggcac atctgtcgta tgcggccctg gccagctgcc
     1561 ccagcaaacg tggtagcgaa tgcgatcttc gggaatgcct tcaagagcgt ggttcaaaac
     1621 ttccacgtgc cggccaaccc acttgtaata atcttcaaaa cttgcggggg gcaccatgcg
     1681 gtcatacgtg actgctgcgc gcgcgtcgtc cacttgcaat agaaaaccgg catcgacaat
     1741 ggtgcggtac tccgttcgca gcgcttccgc gagagccacc accaattctt cttcgttttt
     1801 ataatattca ttcttgcgat caggaatcgc gctggagggc gcggccactg gcaggaacgc
     1861 ttcttcgacg cttactcctg ccagggcggc tttcaaattg tcgatgtcat tctgaagctc
     1921 ggtgagaccc gtgtatttta cgggacccac gcacaccgct tcctgctgtg tcacgttgga
     1981 ccaagtgcga tcgtttccgc cctcaagttc cgcgtagaat tctttaaaac gctcgcggtc
     2041 cgcgccgcgc gcaaatggat ttgcgcctgc cgcaacgggc cgcatttcaa agccgctgac
     2101 gcgcttcaac gcgtacagcg cccagctggt agacttgccg aattcgccgt cattcaccac
     2161 attgatgcct gcctgtacct gccgtttcac aacttcggcg acagattctt tcaggcactg
     2221 ctcatatgcc tttatgtctg ccgggccgcc cttttcgcgc gcgctcacac gatcaagcag
     2281 ttgcgatgga cggatgagac tgcccacatg tgtcgtaaga attcggtctt tgctgcgttt
     2341 cattcgcgac cgccctatgg tttaaattcg atcagcaggt tgtgaatcgg tactttgccc
     2401 acatcttttg aggcgtgaac caccgccggc ctccaggtta cgacgtctgg aatcagttcg
     2461 cgcgtttcat cttttccgtc cgtccattgt tccaccatcg tgtcgttcag aaaaattgcg
     2521 aggcgcacat tgtggctgtg ctgcggatcg tattcgcccg gctgcatctt ttcttcaaat
     2581 acggtgatgt cctgcgcgtc gtacaaaact ttattgtgcg gtgcggcagg tgtccccgcc
     2641 acgggaggca tcacgttcgg atgggtcggc ttgatggaaa tttccaaaaa gtttccagcc
     2701 ttcggagccg cgtagtgttc acccgctttg aatcctttga tgtcgccgcg ctccattttc
     2761 tgaccattta actccagcgg cccgagggcc accactacgc gctcgccgca agaggccgcg
     2821 gccgcagcgc atggcgcgct atttttaaac acgcgcacaa actcattgtc aacaagtgga
     2881 gcgcctagcg cggcctgaag aaaaatcgtt gccacaagca gtagtgtcat tttttatttt
     2941 gcccttctct gaggcacaag tattgcgcgt agtgtacagc aagtcagagg gttttgtagc
     3001 acaggcttca gcctgtggaa tttagtcctt gcaaggacga acccccacag gctgaagcct
     3061 gtgccacgta gcccgcacga tgacggcttg taaaaaaaaa gatcttcaca cagttaaaag
     3121 ctttgcgatg acgtccgcgg cctcatacga aggtttatcg gcaggcagaa agcgcccgag
     3181 cgttgccaaa tcattcccgg atgtcaccac cccgggttcc gttaaattgc cgttgcgcac
     3241 ttgcggatgc ccaatcgtcg cgagcagcgc attgcacagg caccgctttc ccgcagtgtc
     3301 ctccgctttt ccacccttac tgacgtatac cgaaaccggt tcgcccgcgc agcggtaatc
     3361 gatctggccc tccggcgtgc gataggtttc gcgcaaatac cccaggtcgc agatgcgcgg
     3421 ccgctccaga taaatttcct cttcggataa ggtgccgtcg agttgtgcca ccttgaaagg
     3481 aaaacttgta ggagaggcag tgggatcagt ggtcacgcga atttctgaat gcgaaacttt
     3541 ttccagcaca gcacgcttgt agtcttccct caagccggat tcttcgcaga atgcgaatgc
     3601 cgtgcccact tgaatgcccg ttgcgccaac gctcaaagct tcgcgcaatt tttccgccga
     3661 gccatagccg cccgcgagcc agaacggttt tcccagttgg cgaatttttt cgagatcgac
     3721 cacgtcgcgc tctccgtaaa tcacgtcgcc gttttcgtcg agttgcaatt tcccgcgcgg
     3781 cggcgcgttg tggccgccag ccgtcggccc ttcgattaca aagccttcta cgctgccgct
     3841 cgctttcttt aacatcgcag cggccaatac gttcgacgcg atgatcgcca gaaatcgggg
     3901 acgagtgagc ggaggaagtt cgcccggcac gaagcttttg ggatcgaatg acatttccgc
     3961 ttcttggcct tcttgcgagc ccgttgcgtg cagcggatag gttgccgctt catgatgaac
     4021 aaagcgatcc agcacgcccg gaatttttac aggaattccc gcgcccatca gcacatatcc
     4081 gacgcccgcg agcatcgcgc catagatgga gggaagatgc ggaatctgaa ttttttcaag
     4141 atagttaatg ccgacaggat tgtcatggcc ttcgcgcgcc aaaaaaactt ccacaaagtt
     4201 ggccaccaca cagagttctt cgcgatcacg cggaccgtct ttgtcgtgca ttggcatcgg
     4261 cttatatgga gtgcgttcac tttttccacc gggaagaaaa tattccagct cgatgcgctc
     4321 ggccattttt cgagcaggga agtgagccat tgcgcggcgc atgtgtccgc cgggatctcc
     4381 atcttgcaaa cgcctcgcca ggatctgatc aagagcggtt ccagaaacga cgcccagatg
     4441 gccaagtttg gagaccgcct gcgccaggcg ccaatttgaa acacctgcgc ccatgccgcc
     4501 ttgaataatt ttcggataat cgctcataca cttattattc ccacgccgga agaaatcctg
     4561 ttgtaacact tttgtaattg taaaccggtt aattccagga gagatgtgat gagcgtcacg
     4621 gtctaagtgg cgcgggcttc agcctgcggg gtttagtcct tgcatggcct aatttcacag
     4681 gattcgtctt tgcatgtacg aaaccgcaga cggctacccg tcggaacact caggataaaa
     4741 agccggagct gctctgatgc gcaagtcgta gtcgctcaat tcaacatgcg ccgcgcgctc
     4801 tccagcccgg cacgcgcaag agcataccga ggctcgatgt tcagcgactc ctgaaaaagt
     4861 tcgcgggcac gattgatcag ccctttgtga agataaaccc gtccgaggtt gtagtaagga
     4921 aagtgtcgtg gatcgtagcg tttcgctttc atggcctgct cgagccaggg aatcgcttca
     4981 tcgtggcgat tgaggtcgat caggtacacg ccaatgtcgt tataaggatt tccgaaatca
     5041 ggatcgattt gaatggcgcg cttgcattcc gcaatcgctt cttcgatttt tccctggaaa
     5101 tgataggtcc agccaaggaa cgtgtaagcc tccgccgtag ggaacagctc gatggacccg
     5161 agataaagct cgatggcgcg atcgagttct cccgccatct gcgcggcaaa cgcatcctgg
     5221 aacctctgcc gcgcttctct ttcccgttct gacgacatgg gccatttaga ccacagcgcc
     5281 tggggcctgt caatgatgcg tccgcggtgg cgaagcgttc gatctgattg cgtgctgagc
     5341 acgcgagaac gagtgcccgg tcaaaatgca ggcagccgct acgaaagcaa gcatcgcctc
     5401 gaacaactac attttcgaag cgccgaaccc gtcgacgaat tcaggcgttc cctcagtcgc
     5461 ctgaacagaa gcgaccgtcg gcttgcggaa gtcggtggtc gcaaccttta ggagacagga
     5521 gcatgccagt ctgcaaactt tgcgaacggg actttacaca ggaagaaatc gacaacggtg
     5581 tgacgctacc ccacacaacg gcgtcgagaa ttcgattagt cagattttca aatgggacgc
     5641 ttcatcagtt tgagaagaaa tcaaacgtcc agttgccgca cggcacgtac gtcacggcga
     5701 agccgacgcc agtcgaggct ccgctggcgg aagtcgaggc tttgacagac ccggtggagc
     5761 aaccacccca agctatcgaa acccccgacg ccgggcagca accagacgtc gaatcgttgg
     5821 cgcatgacct tgacgcccac ttccacccag aacctgaatt accggaagcg aaaaatatcg
     5881 gacttaataa agcagaagca gacgatggat atgttgactg ctttgtggtt ttcatgactt
     5941 cgcatgacac gcaggggcgc ggaagaggcc ccattggaat cgcacgaaag gtaaaccgaa
     6001 ggagagacaa tcacgacgga tttagatttc gcgtcgcgga tgtcgtgacg acaggaggcg
     6061 aaacaatcaa acccggttcg aaaatccgcg gtcgagtagt cgcggattcc gagtacgagc
     6121 gcctcatcga cattgaggtt tacattccgc aggaaaaatc aaaacggcca ccaagcccag
     6181 taaagaaaag agagccagca ataaaaatct ataagcacga cgaggaggag taatgggata
     6241 cgaagaaaca cggcagcatc aggaacggct agcagatgcc atagaaacgg caacgaacgc
     6301 cgccacggaa gtcaaggtct ggatgacgag acacccggag gtcagggata ttttggcgaa
     6361 ctatcaa
//
LOCUS       DASZMB010000266         5246 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3836462, whole genome shotgun sequence.
ACCESSION   DASZMB010000266 DASZMB010000000
VERSION     DASZMB010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3836462"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VG272_12365"
     CDS             complement(<1..368)
                     /locus_tag="VG272_12365"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HWF14526.1"
                     /translation="MKKTGAQILWECLEQQGVTTVFGYPGGAILPAYDALKYSNIHHV
                     LVRHEQGATHMADGYARATGRVGVAIATSGPGATNMVTGLATAMLDSSPIVCITGQVG
                     SKLIGSDAFQETDITGVTLPV"
     gene            complement(585..1616)
                     /gene="tdh"
                     /locus_tag="VG272_12370"
     CDS             complement(585..1616)
                     /gene="tdh"
                     /locus_tag="VG272_12370"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF003808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HWF14527.1"
                     /translation="MKALVKSRAAEGLWLEDVPEPSPGINDVKIRVLATGICGTDLHI
                     YDWNEWAQNTIHVPLVIGHEFCGEVVEMGSNVADLQPGDFVSGEGHVVCGRCRNCLAG
                     RRHLCAHTQGVGVNRAGAFAEYVVLPMMNVWKHTRPIAREVAAIFDPFGNAVHTALSF
                     PVLGEDVLVTGAGPIGIMAAAVARFSGARHVVITDVNPNRLALAERAGIEITVDSRTT
                     RLQEVQKRLDMREGFDVGLEMSGSPEAFREMLKNMCHGGKIAMLGIPASEIAIDWSHI
                     IFNMLTIKGIYGREMFETWYKMTVMIESGLDISPVITHRFPWQEFEKGFEVMRTGEAG
                     KVVLDWSNV"
     gene            complement(1852..3690)
                     /locus_tag="VG272_12375"
     CDS             complement(1852..3690)
                     /locus_tag="VG272_12375"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha/beta"
                     /protein_id="HWF14528.1"
                     /translation="MLRGKTNVPSTVKCPGCATAQARAALEENLYICPGCNHYLTMPS
                     RARIALLADTRTFRELDRDLVSVDPLGFTDHKSYRVRLQDARRETGVREAVTAGFCRI
                     GGQQAVLVVFDFAFLGGTMGSVVGEKIAHAFEEATQRRIPLVSVAASGGARVQEGMFS
                     LMQMAKVAAAASVHDRHDLAFISILTDPTFGGVTASFASLGDILIAEPNAQIGFVGPR
                     VIEQTTGVAPPKDSHHSDTLLKAGLIDLVIGRRDLPAITAYLIGHLKRNKAMEPGVKQ
                     LPPPVRSKHITAWQKVNLARHAGRPTSKYYIAHMTSRFLELHGDRWSGDDPAVVGGIA
                     ELDRETVIIIGQERGATPEEKIKSNDGKAYSEGYRKSLRLMRLASKFKIPVITLVDCP
                     NAQASYESEHRGIAYSLARNLATMANLPTPIVSAIIGEGGSGGALAMGVADRVLMLEN
                     AIYSVISPEGAAAILYRDAGKAEAVSDVLKLTAQDLHSLGIIDTMVPESEGGAHLDPA
                     AAAETLKAHLLAALRPLTELPMRQLLDARYKKYRHIGEEGKYWQEQVRSGISDVFGLL
                     AYAVSKMERSMDKSSRKPKPADGTPRRRLDKAKLPSATGLKRAERE"
     gene            complement(4127..4987)
                     /locus_tag="VG272_12380"
     CDS             complement(4127..4987)
                     /locus_tag="VG272_12380"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWF14529.1"
                     /translation="MALPVYQQVFSMQVRSEIPELLVDPNLHSFPPSYPASPDTRNGP
                     AAKAATATNRVAPHRAGSLSRYDGRAVFDAVCAAAGLVAMAPLFVMIAVAIKLDDGGS
                     IFYSHLRIGRNFQKFRLYKFRSMISSNVEGSTVTSPGDARVTRIGAFLRAFKLDELPQ
                     LINVLMGDMQLVGARPQMEKFVEIFCDEYGELLESRPGITDLASLIFRDEAKFFYEGS
                     IEEQYVKRIMPIKLEVSLKYARTRTFFSDMDIILRTVLGMSCPPTAWENTTFDPAKHA
                     YPEIISRKSS"
     gene            complement(5043..>5246)
                     /locus_tag="VG272_12385"
     CDS             complement(5043..>5246)
                     /locus_tag="VG272_12385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWF14530.1"
                     /translation="GSYLGVSIGSAVLVARRKGWKFLPILPVVFATYQLPYALGFLAG
                     VFYQGRGPRLPKSSPKAIVLNSR"
BASE COUNT         1182 a         1519 c         1365 g         1180 t
ORIGIN      
        1 acggggagag taacgcccgt aatgtccgtt tcctgaaatg catccgagcc gatcagcttg
       61 cttcccacct ggcccgtgat gcacacgatg ggcgatgaat cgagcatggc ggtggcgagg
      121 ccggtcacca tgttggtcgc gcccgggcct gaggtcgcga tagcgacgcc gactcgtccg
      181 gtcgcgcgag cataaccatc cgccatgtgc gttgcgccct gttcatggcg caccagtacg
      241 tggtgaatat tgctgtactt caacgcatcg taggcgggca aaatcgcgcc gcccggatag
      301 ccgaatacag tcgtcacgcc ctgttgttcc aaacattccc aaagaatctg cgcgcctgtt
      361 ttcttcatat gatgatccgt ccggaataag tctggctgcc cactcgttta aaatgaaccg
      421 catgcgctac cgccgagcaa gcgggctaca cttgtactta caactttttc aaattttgca
      481 gaaccacccc atcccccggg atagcctgtt tgcacccgaa cattatacag agaaattgga
      541 gtatcgtctt gttgggattg aaaaacgtgc agactgccct cggatcagac gttcgaccaa
      601 tcaagtacta ctttgcccgc ttcgcccgtg cgcataacct caaagccctt ctcaaactcc
      661 tgccacggaa aacggtgcgt aatcacggga gaaatatcca gcccggattc gatcatcacg
      721 gtcattttgt accaggtttc gaacatttcg cggccgtata tccccttgat agtgagcatg
      781 ttgaaaataa tgtggctcca gtcaatggcg atctcgctgg ctgggattcc gagcatggcg
      841 atttttccgc catggcacat gtttttaagc atttcacgaa atgcctctgg tgaacctgac
      901 atttcaagtc ccacgtcaaa cccttcccgc atatcgagcc gcttttgcac ttcctgcagg
      961 cgcgtcgttc gcgaatccac tgtgatctca atccccgcgc gttccgccag cgcgaggcgg
     1021 ttcggattta cgtcggtaat tacgacgtgc cgcgcaccag aaaaccgcgc caccgcggca
     1081 gccatgatgc cgatcgggcc cgccccggtc accaatacgt cttccccaag cacaggaaat
     1141 gacagcgcag tatgcacggc gtttccaaac ggatcgaaga tcgcggcaac ttcacgagcg
     1201 atggggcgcg tgtgtttcca cacattcatc atcgggagca caacatattc cgcaaacgct
     1261 cccgctcgat tcacgccgac gccttgcgtg tgcgcgcaca ggtggcgccg tcccgcgagg
     1321 caattgcggc aacgcccgca tactacatgc ccttcgccgc tgacgaaatc gccgggctga
     1381 agatccgcta cgttcgatcc catttccacc acttcgccgc aaaactcatg cccaatcacg
     1441 agcggtacgt ggatcgtgtt ctgcgcccac tcattccaat cgtaaatgtg cagatcggtg
     1501 ccgcaaattc cggtggcgag cacgcgaatc ttcacatcat tgattccggg cgacggctcc
     1561 ggaacatctt cgagccacaa cccctctgcg gcacggctct tcactaaggc tttcattctg
     1621 gatgtccttt ggcggagtcc cgggcgaaac gaacccgcaa atcaccaacc gatagaaatc
     1681 atctcaaaac cgtatcccgc cgacggtccc caaagcaaat atcggtgagt cgtcgagtag
     1741 cgctgccact cttggccgtg tgcactttgc aaaacccgca caggcgactg cctgggctac
     1801 tcaaaatttc acccaaacga ttttttccgc agacgggctc tcgccgaatt tttactcgcg
     1861 ctctgcccgc ttcaagcccg tggcagaagg cagttttgcc ttatctaacc tccgcctcgg
     1921 cgtgccatcc gctggcttgg gctttcggct agatttatcc atcgatcttt ccatcttgga
     1981 tacggcatat gctaaaagcc caaacacgtc gctgatgcca ctgcgaacct gctcttgcca
     2041 atacttccct tcctctccga tatggcgata ctttttatac cgggcgtcga gaagttgtcg
     2101 cattggaagc tcggtgagcg ggcgtagggc agccagtaaa tgggctttga gggtttctgc
     2161 cgccgcggcc ggatccagat gtgcacctcc ctcggattcg ggcaccatgg tatcgataat
     2221 tcccagggag tgcaggtctt gcgctgtcaa tttcaaaacg tccgacactg cttcggcctt
     2281 gcccgcatcg cgatacaaaa tcgccgcggc gccttcgggt gaaatcacgg aataaattgc
     2341 gttctccagc atcagcacgc gatccgcaac gcccatcgcc agcgccccgc cactcccgcc
     2401 ttccccgatg attgcggaga ctatgggcgt aggcagattg gccatcgtgg ccagattgcg
     2461 cgcgagcgaa tatgcgatcc cgcgatgctc ggactcatag ctcgcctgcg cgttcgggca
     2521 atctacaagt gtaatcacgg gaattttaaa ttttgaggcc agccgcatca ggcgcagcga
     2581 tttgcgatag ccttcggaat acgctttgcc atcattcgat ttgatttttt cttccggcgt
     2641 ggctccgcgc tcctgaccga tgatgatcac tgtctcgcga tccaactccg cgatgccgcc
     2701 aacgaccgca ggatcgtcgc cgctccagcg gtcgccatgc aattccagaa aacgcgaggt
     2761 catgtgggca atgtaatatt tcgaggtcgg ccgccccgca tgcctcgcaa ggttcacttt
     2821 ttgccaggcc gtgatgtgct tggatcttac aggcggcggc aattgcttta cgcccggctc
     2881 catcgctttg ttgcgcttca ggtggccaat cagatacgca gtgatcgcgg gcaaatcgcg
     2941 gcgcccgatc accagatcga tcagccccgc tttcagcagc gtatccgaat ggtgcgaatc
     3001 cttgggtggc gcaacgccgg tcgtctgttc gattacgcgg ggacccacga atccaatttg
     3061 agcgttcggt tcggcgatca gaatatcgcc aagcgatgca aagctcgcgg ttacgccgcc
     3121 gaacgtcgga tcagtcaaaa tggagatgaa ggccaggtcg tgcctgtcat gtacggaagc
     3181 tgcggccgca accttggcca tctgcatcag ggagaacatt ccttcctgca cgcgggctcc
     3241 gccgctggcc gccacgctga ccaatgggat tctgcgttgc gtggcttcct cgaaagcatg
     3301 ggcgattttt tctcccacca ccgagcccat cgtgccgccc agaaacgcaa agtcaaaaac
     3361 gacaagcact gcttgctggc ctccaatgcg gcaaaaacca gcggtcaccg cttcccgaac
     3421 accagtttcg cgacgagcat cctgcagccg cacgcgatac gacttgtgat cggtgaatcc
     3481 cagcgggtcg accgaaacca gatcgcggtc gagttcccga aatgtgcgcg tatcggctaa
     3541 caaggcgatt cgcgctctcg acggcatcgt caaatagtga ttgcaaccag ggcagatgta
     3601 gaggttttct tcgagtgcgg cgcgcgcctg cgcagtagcg cagccgggac acttcaccgt
     3661 agacggaacg ttggttttgc ctcgtagcat agttccttag tccttataaa caccggatcc
     3721 tgacgctggt aagcggaata ttgctcttta attatcgcag gttcagcgat aacgattgga
     3781 tgaaaaaatg gaggattgtg attagaagtt caggggtgaa ggcgatcaat agaaaccaaa
     3841 cactaaacgg caggagggaa ggtacttagc ggatttaacg gagggtaact ctttaaggga
     3901 ggagggccga atgtgggtgt tcactcggcc ccggagtggt caggaatacg atctgctcat
     3961 gagtgagatt aggggtggat tccgattttg tccaattcat cgttttgata gattgaatca
     4021 ggtttccggc tcctcttttg gaactcttca cgcacgatgc cagcaaacaa tacttacctt
     4081 cagaaattct tccgcggaat tcattcgcga agacaggtgc gcacattcat gaagattttc
     4141 tggaaataat ttccgggtat gcgtgcttgg ccggatcaaa cgttgtgttc tcccacgctg
     4201 ttggcggaca ggacataccc aaaacggttc gcaaaataat atccatatcc gaaaagaaag
     4261 ttcgcgtcct cgcatacttc agtgagactt ccaatttaat cggcatgatg cgttttacat
     4321 actgctcttc gatggatcct tcgtaaaaga attttgcttc atcgcggaaa atcagcgatg
     4381 ccaggtctgt gatccccggt cgcgattcca ggagttctcc atattcatcg caaaatattt
     4441 cgacaaattt ttccatctgc ggccgcgcac ccacaagttg catgtctccc atcaacacgt
     4501 tgatcagttg cggcagctca tccaatttaa atgctctgag aaacgctcct atgcgagtca
     4561 cgcgggcgtc acccggagaa gtaaccgtgc ttccctcaac attgctgctg atcatgctgc
     4621 ggaatttgta gagccggaat ttttgaaagt tccggccgat acgcagatga gaataaaaaa
     4681 tggaaccacc atcatcgagc ttgatcgcaa cggcaatcat aacgaagagc ggcgccatag
     4741 cgaccagtcc cgccgcagcg cacacggcat cgaacacagc gcgcccgtcg tagcgtgaca
     4801 acgatccggc ccggtgcggc gctacccgat tcgtagcggt ggctgctttc gccgccggcc
     4861 cattgcgggt atcaggcgac gcagggtaac tgggtggaaa gctgtgcaag tttggatcta
     4921 ccagcaattc gggaatttcg gaacgcactt gcattgaaaa gacttgttgg tagacaggaa
     4981 gggccacggg aatagcctcc gaaatcagat ttagtttgaa caggatttgg tccgacgaat
     5041 ttttatctgg agttcagtac aattgccttt ggagacgact tgggcagtcg agggcctcgc
     5101 ccctgataaa aaacccctgc tagaaatccg agcgcgtacg gcagctgata ggtggcaaac
     5161 actacaggca agatcggcag aaatttccac cctttgcggc gcgcgaccag cacggcggac
     5221 ccgattgaca ctccgaggta tgaacc
//
LOCUS       DASZMB010000267         3309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3841467, whole genome shotgun sequence.
ACCESSION   DASZMB010000267 DASZMB010000000
VERSION     DASZMB010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3841467"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VG272_12390"
     CDS             complement(<1..389)
                     /locus_tag="VG272_12390"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HWF14531.1"
                     /translation="MKRTNISTGTKYEPIIGYSRAVRVGDTVHVSGTTATDADGNLVG
                     LGDAYAQTMQTLRNIESALHKAGARMEDVVRTRIFVANIAEWEKIGRAHGEFFGTIRP
                     ATAMVQVGGMVSPEMLVEIEAEAIVMKR"
     gene            704..2107
                     /locus_tag="VG272_12395"
     CDS             704..2107
                     /locus_tag="VG272_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWF14532.1"
                     /translation="MKEPSLRCNNILEAIGHTPLVRLNRLTAGFAADVFVKVDYMNPG
                     GSVKDRIGVSMIEDAEKKGLLKPGGTIIEGTSGNTGIGLALVGAVCGYKLVFTITDKQ
                     SKEKVDLLKALGAEVIVCPTAVEPDDPRSYYSVAKKLAKDIPNSFYPNQYENQMNPEA
                     HYRGTGPEIWEDSEGKITHFVCGMGTGGTISGVGRYLKEKNPNVQIIGVDPIGSLYYE
                     FKKTGKVGAAKTYVVEGIGEDIFPSTMDFNMVDDVVQVDDQECFVWARRLVKQEGIFT
                     GGSGGGCVSGALRVAKSMSKGSFVVAFLPDTGMRYLSKVYSDEWMRERGYVDSEVLLT
                     VEDVVNAKHKVSKDRDLILVGPDQTVFHALHTMQTQDISQLPVFEAKQPIGAIYEDQI
                     LNLALQGKDLRKMVIREIMSDPLPMISCEAPVERLTYLLSHEEPAVFVQMGNGRLEII
                     TKFDVMGTIAGLMEQKR"
     gene            complement(2338..>3309)
                     /locus_tag="VG272_12400"
     CDS             complement(2338..>3309)
                     /locus_tag="VG272_12400"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWF14533.1"
                     /translation="DAGTDQAYTDAIIAAVESGVNVIDTAINYRFQRSERSVGAALVE
                     LARRGYGREELLICTKGGFLTPDGDMPADATEYFNREYVDTEILREGDVAAGCHSIAP
                     KFIADQLERSRRNLGVETIDVYYLHNPETQLTEVPREMFNTRVRAAFEFLESAVAAAK
                     IHFYGLATWNAFRQGENEQDFLSLVAMESLARDVAGGSHHFRFVQLPCNLGMTEALTR
                     ANQELDGARVPVVGAASKMGITLMASASMLQGQLTKGLPQFVKNALELNTDAERAMQF
                     VRSTPGIATALVGMSRVEHVRENLSIVGVPPASQEQFAKLFQRGEKA"
BASE COUNT          759 a          886 c          910 g          754 t
ORIGIN      
        1 cgcttcatca cgatggcttc cgcttcgatt tccaccagca tttcgggcga gaccatgccg
       61 cccacctgca ccatcgccgt ggccgggcga atcgtgccga aaaattctcc gtgcgcacgg
      121 ccaatctttt cccactccgc aatgttggcc acgaagatgc gcgtgcgtac aacgtcttcc
      181 atccgcgcgc cggccttgtg cagcgcggat tcgatgttac gcaacgtttg cattgtctgc
      241 gcgtaggcat cgcccaagcc cacgaggttg ccatccgcgt ccgtggctgt tgtgcctgag
      301 acgtgcaccg tatcgcccac gcgcactgct cgcgaatatc cgatgatcgg ttcgtatttc
      361 gttcccgtag aaatgttggt ccgcttcatg gctccgtctt ctcctggttg agagtgtggc
      421 ctattctgcc acgaacctgg gcgcgttgag aaactggaca accgcttggc tgtgcactta
      481 agctggaatc gccggagaaa agctttaaca cagaggacac agagaaaaga ggatcaaggc
      541 ggagaaagac gagcgcggga gattgaaaaa aacttttcca ttcagaggga tgatcggcaa
      601 ttgaaatgcg acgcgctcgg ttcgttgttt tctccctctt tctcctcttt tctctgtgtc
      661 ctctgtgtta aagcttttga atttttgcca cacccagccg tagatgaagg agccttcgtt
      721 gcgctgcaat aacattctcg aagccattgg ccatacgcct ctcgtacgcc tgaaccgcct
      781 gactgccgga tttgccgcgg atgttttcgt caaagtcgat tacatgaatc cgggcggctc
      841 ggtgaaagac cgcatcggcg tctccatgat cgaagatgcg gaaaaaaagg gattattaaa
      901 acccggtggc acgataatcg agggaacctc cggcaacact ggaattggcc ttgcgcttgt
      961 gggcgcagtg tgcggctata aactggtgtt caccatcacg gataagcaat ccaaggaaaa
     1021 agtggacctg ctgaaggcgc tgggcgcgga agtgatcgtt tgccccacgg cggtcgagcc
     1081 ggacgatcct cgcagctact attccgtcgc caaaaaactc gcgaaagata ttcccaattc
     1141 attttatccg aaccaatacg aaaatcagat gaatcccgag gcgcattacc gcggtacggg
     1201 cccggaaatt tgggaagact cagaaggaaa aatcacgcat ttcgtttgcg gcatgggcac
     1261 gggaggcacg atcagtggcg tgggccgcta cctgaaagaa aaaaatccca acgtgcaaat
     1321 tattggcgtc gatcccattg gatctctgta ttacgaattc aagaaaaccg gcaaagtggg
     1381 cgccgcaaaa acgtatgtgg tggaaggcat cggcgaagat attttcccgt ccacgatgga
     1441 tttcaatatg gtcgatgacg tggttcaagt ggatgaccag gaatgtttcg tttgggcgcg
     1501 gcggctcgta aaacaggaag gaattttcac cgggggctcg ggcggcggct gcgtttccgg
     1561 cgcgttgcgc gtagccaaat cgatgtccaa gggttctttc gtcgtcgcgt ttttgccgga
     1621 cacgggaatg cgctatctga gcaaagtgta tagcgatgaa tggatgcgcg agcgcggcta
     1681 cgtcgattcg gaagtcctgc tcaccgtcga agatgttgtg aacgccaagc acaaggtcag
     1741 caaagatcgc gatttaattt tagtcggacc ggaccagact gtatttcacg cgctgcacac
     1801 catgcagacg caagacattt cgcaactgcc cgtctttgaa gcgaagcaac ccatcggcgc
     1861 gatttacgaa gaccagattt taaatctcgc gttgcagggg aaggaccttc gcaaaatggt
     1921 gatccgcgaa atcatgagcg atcctctgcc catgatttcc tgcgaagctc ccgtcgagcg
     1981 gctaacttat ttgctgagcc acgaagagcc cgctgtattc gtgcagatgg gcaatggccg
     2041 cctggaaatc atcaccaagt ttgacgtgat gggcacgatc gcaggattga tggagcagaa
     2101 gcggtgacgt aggataggct ttacaggttg cggaaaaagt cgtttattct gtcattccga
     2161 gcgaagcgag gaatctctct tcgatttaag tataagaaaa taaagaagga gggattcctc
     2221 gcttcgctcg gaatgacaaa atgttgagtt tttccgcagc ctatttagcc tgtgggtttt
     2281 aattgaatag ctacgcaaaa tttaaatcca caggctgaag cctgtgctac ttaagtccta
     2341 tgctttctct ccgcgctgaa atagcttcgc gaattgttcc tgcgaagcgg gcgggactcc
     2401 gacaattgaa agattctccc gcacatgctc gacgcggctc atgcccacga gagccgtcgc
     2461 gattcccggc gtagatcgca caaattgcat agcgcgttcc gcatcggtat tcagttcgag
     2521 cgcatttttc acaaattgcg gaagtccctt ggtgagttgc ccctgcaaca tcgaagcgct
     2581 ggccatgagg gtgatcccca ttttggatgc cgcgcccaca accggcactc gcgcgccgtc
     2641 gagttcctga ttagcgcgtg tgagtgcctc cgtcattccc aggttgcacg gcagttgcac
     2701 gaagcgaaag tgatgcgacc ctcccgcaac atcacgcgcc agcgactcca tcgcaacgag
     2761 cgaaagaaaa tcctgctcat tttcaccttg gcgaaaagcg ttccatgtcg cgagtccgta
     2821 gaaatgaatt ttcgcagcgg ccacggcgga ctccagaaat tcaaacgcgg cgcgcacccg
     2881 cgtgttgaac atttcgcgcg gcacttcggt caattgcgtt tccggattgt gtaggtagta
     2941 gacgtcaatc gtttcgacgc cgagattgcg gcggctgcgc tccagctgat cggcaataaa
     3001 tttcggcgct atcgagtggc aaccggcggc gacatcgcct tcgcgcaaaa tttctgtatc
     3061 cacatattcg cgattgaaat attcggtggc gtcggcgggc atgtcgccgt caggagtaag
     3121 gaatccgccc ttcgtgcaaa tcagaagttc ttcgcgaccg tagccgcgcc gcgccaattc
     3181 cacgagcgcc gcgcccacgg aacgctccga gcgctgaaag cgatagttga ttgcggtgtc
     3241 aatgacgttc acgccggatt cgacggccgc gataatcgcg tccgtgtagg cttgatccgt
     3301 gcccgcgtc
//
LOCUS       DASZMB010000268         6403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Candidatus Acidiferrales bacterium isolate SMAG_U5880
            k141_3842645, whole genome shotgun sequence.
ACCESSION   DASZMB010000268 DASZMB010000000
VERSION     DASZMB010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37956700
            Sequence Read Archive: SRR11842680
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Acidiferrales bacterium (soil metagenome)
  ORGANISM  Candidatus Acidiferrales bacterium
            Bacteria; Acidobacteriota; Terriglobia; Candidatus Acidiferrales.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-DEC-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/01/2024 08:12:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,484
            CDSs (total)                      :: 2,455
            Genes (coding)                    :: 2,449
            CDSs (with protein)               :: 2,449
            Genes (RNA)                       :: 29
            tRNAs                             :: 25
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 3 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Candidatus Acidiferrales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3842645"
                     /isolate="SMAG_U5880"
                     /isolation_source="soil"
                     /db_xref="taxon:2952895"
                     /environmental_sample
                     /geo_loc_name="USA: California"
                     /lat_lon="36.5 N 121.8 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            488..1327
                     /locus_tag="VG272_12405"
     CDS             488..1327
                     /locus_tag="VG272_12405"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWF14534.1"
                     /translation="MPRITIRDGTQIYHKDWGTGQPVFFSHGWPLSADAWEDQMMFLA
                     DHGYRCIAHDRRGHGRSGQPWNGNHMDTYADDLAAIVEALDLHDVIHVGHSTGGGEVA
                     RYIGRHGTKRVAKAVLISAVTPLMLKTPANPKGLPIEAFDKLRTAVKTDRSQFFKDLS
                     EPFYGANRPGSKVSQGLRDSFWLQGMQCSLKAAYDCIEAFSESDFTEDFKKFDVPTLI
                     LHGDDDQIVPIEDTALLSAKLVKGATVKIISGAPHGMCSTHKDQINAELLSFIQRSKQ
                     ASA"
     gene            1336..1773
                     /locus_tag="VG272_12410"
     CDS             1336..1773
                     /locus_tag="VG272_12410"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWF14535.1"
                     /translation="MENSEVRTVYVRGSGKGLAQQIQIAPHCIFSDEPASAGGTDTGP
                     TPYDLLLGALGACTSMTVTMYARRNAWPLEDVIVHLRHSRIHAEDCASCEAKEAMLDQ
                     IERDIHISGKLSDEQRSKLLEVANKCPVHRTLTSKIHIETRMV"
     gene            1827..2315
                     /locus_tag="VG272_12415"
     CDS             1827..2315
                     /locus_tag="VG272_12415"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 family protein"
                     /protein_id="HWF14536.1"
                     /translation="MSFSTVLISALLIGFVCGLRALTAPAVVAWAAHRNWIDLSNTRL
                     HFMSTTAAVAIFTLLALGEFIADQLPSAPNRTDPAGLIPRIFLGALCGAALLASETQS
                     VAIGSLLGAAGGVAGAFSGYFVRTRLVKNFKIPDFVIATLEDVLAIGAGIFVVSRFYF
                     HS"
     gene            2964..5807
                     /locus_tag="VG272_12420"
     CDS             2964..5807
                     /locus_tag="VG272_12420"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF005122.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HWF14537.1"
                     /translation="MSQTKVAINEKSALQAGVPTPVGTETRTGLVFERYFTDGRMSPF
                     DAVEWERRVALIGNEKGKTIFRQEDVEVPKGWSQTATNIVASKYFHGKPNTPERENSV
                     RQLIGRVVDSIVNWGEKGNYFAGTSSRDAFRDDLTHLLVEQKMAFNSPVWFNVGVQAK
                     PQCSACFINSVQDNMESIMNLAKTEGMLFKWGSGTGTNFSSLRGSRETLSGGGIASGP
                     VSFMKGFDAFAGVIKSGGKTRRAAKMVILNVEHPDIVEFIDSKMKEERKARVLIEQGY
                     DPAIDGEAYSSIFFQNANHSVRVTDEYMQSVEEDRDFWTRNVNDGNPVEKFSARELLH
                     SMAESTWQCGDPGMQYDTTINRWHTCKTTARINASNPCSEYMFLDDTACNLASLNLMK
                     YLNSNGSFDVEAFRHAVDVTITAQEILVDNASYPTPRIADNSHKFRPLGLGYANLGAL
                     LMSMALPYDSDGGRDMAATVTALMCGEAYAQSARIAERLGPFAGYAVNREPMLEVIRM
                     HRDSMRPIKPESVQPSLLGAAQASWDSALSLGEKFGYKNSQVTVLAPTGTIGFMMDCD
                     TTGIEPDLALVKYKKLVGGGVIKIVNNTVPAALMKLGYTPEETSQIVSYVDRNGKIEG
                     APGFKPEHLAIFDCSLAAPGGRSIAWRGHLKMMAAAQPFLSGAISKTINMPEESTVED
                     IMQAYIESWKLGVKAVAIYRDNSKGSQPLSAAGDKKDTAQQTQPAASAAAAAATAVAE
                     QQELFVRSRRRKMPSERQSITHKFSIGGHEGYITVGMYETGEPGEIFIKMAKEGSTLS
                     GFMDGFALSVSIGLQYGVPLKALVDKFINTRFEPAGYTGHPAIPYAKSVLDYLGRWLG
                     GRFISPDYLTHNGEAALPAATVTAMPAVPPAFIPTADDDPSPASARSTATVRSDARPM
                     FDGAPTCSECGMLMTPNGACYKCENCGGTSGCS"
BASE COUNT         1499 a         1844 c         1685 g         1375 t
ORIGIN      
        1 tgaaaatcac aatttgccga gagcattggt cgcttcgctg ctattgcgtc accttcaatg
       61 taagtggatt tggcgtggaa acgggtgtgc cgctcgcgcc attcgctgaa tattcgatcg
      121 ccctctacaa taaattacgg atagactgcc tccatgatgc gcgagcatta ttacagggtt
      181 gccaagggtt tggccggaaa aacgactcgc gaccgggtgg attggaattt aataaacggc
      241 tccgtttaaa tttagaaacg aatgccggta attttgagcg cgcattgcct gaatctgtcg
      301 gcaaagtttt tgattgcttg atctaagaat gatctatgaa ttttgagcca gccttcaagc
      361 ccctaacgat cgcgacccag cacgcgcggg cataacgtaa aaataaatcg cagcgcccgt
      421 attccgccac aatggccttt gtttttttag cggcgcaggc gtaaagtcgc cataaaagga
      481 gacaccgttg ccacgaatta cgatcagaga cggaacgcag atctatcaca aagattgggg
      541 taccgggcaa ccggtcttct tcagtcatgg ctggcctctc agcgcagatg cctgggaaga
      601 ccagatgatg ttcctcgccg accacggcta ccgatgcatc gcgcatgacc gccgcggcca
      661 cggccgatcg gggcagccct ggaacggcaa ccacatggac acatacgctg atgatcttgc
      721 ggcgattgtc gaagcgctcg atctgcacga cgtgatccac gtgggccact ccaccggcgg
      781 cggcgaagtc gcccgctata ttggacggca tggcacaaaa cgtgtcgcca aagccgtatt
      841 aatttccgcc gtaacgcctc ttatgctcaa aacgccggcg aatcccaagg gtttgccgat
      901 cgaagcattc gataaactgc gcacggcagt caaaacggat cgctcgcaat tttttaagga
      961 tttgagcgaa cctttttacg gagccaaccg gcctggatcg aaagtttccc agggattgcg
     1021 agactctttt tggttgcaag gcatgcagtg cagcctcaaa gccgcctacg actgcatcga
     1081 agccttttcc gaatcggatt tcaccgaaga cttcaaaaag ttcgacgtac caactctcat
     1141 ccttcacggt gatgacgatc aaattgttcc catcgaagac acagcgcttc tgtcggcaaa
     1201 gctggtcaaa ggcgcgacag taaaaattat ttcaggcgcc ccgcatggaa tgtgttccac
     1261 gcacaaagac cagatcaatg cggagcttct ttcattcatc caacgcagca agcaggcgtc
     1321 agcctaggat aaacgatgga aaatagcgaa gtgaggactg tgtatgttcg cggaagcggc
     1381 aaaggtcttg cgcagcaaat tcaaatcgcg ccgcattgca tcttttcgga tgagcccgct
     1441 tccgcgggcg gcacggatac gggtccaact ccctatgacc tgttacttgg cgcactcgga
     1501 gcatgcacgt cgatgacggt aacgatgtat gcgcgccgga acgcgtggcc ccttgaagat
     1561 gtaatcgttc atcttcgcca ctccagaatt catgccgaag actgcgccag ctgtgaagcg
     1621 aaagaagcaa tgctcgatca aattgagcga gacattcata taagtggaaa attgtccgac
     1681 gaacagcggt ccaaacttct ggaggttgcg aacaaatgcc cggtgcatcg aaccttgacg
     1741 tcgaagatcc atatcgagac gcgcatggtt tgaaagaatg atttgtgttt gaaaatgtga
     1801 ctggcaaacg attgaggtag agttagatgt cgttctcaac ggtcctgatc tcggcgcttt
     1861 taattggctt tgtttgcggc ttacgcgctc ttactgctcc agccgtcgta gcttgggctg
     1921 cccaccgaaa ctggatcgat ctcagcaata ctcgtcttca ttttatgagc acgactgccg
     1981 cggttgcgat ttttacgctg ctcgcactcg gagaatttat agccgaccag cttccttccg
     2041 cgcccaacag gactgacccc gcgggattaa ttcctcgaat ttttctcggc gctttatgtg
     2101 gtgccgcgct tttggcatcc gaaacgcaat ccgttgcgat tgggtctctc ctgggcgcgg
     2161 cgggcggcgt tgctggcgca tttagcggat acttcgtgcg cacacgcttg gtgaaaaatt
     2221 ttaaaattcc agattttgtg attgcaactt tggaggacgt tttggcaatt ggcgcgggaa
     2281 tttttgtagt gtcgcgattt tatttccaca gctgattttc ttacaatcgt cgcggcgtgt
     2341 tacagttcca agagagcaaa agagaatgat cggttctgaa aatgctttta caagattgtg
     2401 tcatttcgga acccaaccac tagggataga cattttaaac acggagtaac aagattttgt
     2461 gcttagcgcc acatttccct cttgacagaa ttttcgctct tgtttacatt gcgccttcgg
     2521 attcagggac cgtgattcga caagtgggcg gcccactcat ccacgggaag tacccaaggt
     2581 cgattaacgc aacccgcaga gtttcacaga gacatcgagg aggctttctt tttctccccg
     2641 tagccggcgc tcccagcgcg gttggcgagg gaatgcatca cgctaagcgt cgctgacggc
     2701 cgcggcgaga tgcaggggga gggctttttc cttttttcga gtgcggactc cattttcgtg
     2761 gattgaatca gccgggggag caagtggcca aggcagatga ccaaggttga tgaagtgacc
     2821 aggatgttcg taaattcctg gtgttaggta ttggaaaaaa gcagcggcct gccgagaatc
     2881 aggtagctgc gtgctcccgg tttcgggagt aaaataaaag ggttccctct gcgcggggga
     2941 gcctaactgc gcggaggcat acgatgtcac aaacgaaagt ggctataaac gaaaagtcgg
     3001 cactgcaagc tggcgtacca acgcctgttg gaaccgagac ccgcaccggc ctcgtattcg
     3061 agcgctattt tacggacggg cgcatgtcgc cattcgacgc agtcgaatgg gaacgccgcg
     3121 tagccctgat cggcaacgaa aagggaaaaa ccattttccg ccaggaagat gtggaagtgc
     3181 ccaagggctg gtcgcagacc gccaccaaca tcgtcgcgtc aaaatatttt cacggcaaac
     3241 ccaatacgcc cgagcgcgag aattccgttc gccagctcat cggacgcgtc gtggattcga
     3301 tcgtcaattg gggagagaag ggcaactact tcgccggcac atcttcgcgt gacgccttcc
     3361 gcgacgacct gactcatctt cttgtcgagc aaaagatggc tttcaattcg ccggtctggt
     3421 tcaacgtcgg cgtgcaagcc aagccgcaat gctcggcctg cttcatcaac tcggttcagg
     3481 acaacatgga atcgatcatg aacctcgcca agaccgaagg catgctcttc aagtggggct
     3541 ccggcacggg cacgaatttt tcttcgctcc gcggcagccg cgaaactctt tccggcggcg
     3601 gcatcgcttc tggccctgtc agtttcatga aaggctttga cgcgttcgcc ggcgtcatca
     3661 agtccggcgg caagacgcgt cgcgccgcca agatggtgat cctcaacgtg gagcatccgg
     3721 acatcgtcga gttcatcgat tcgaagatga aagaagagcg caaggcgcgt gtcctcatcg
     3781 agcagggcta cgatccagct atcgacggcg aagcctactc ctccatcttt ttccagaatg
     3841 ccaaccactc cgttcgcgtc accgacgaat acatgcaatc agtggaagaa gatcgcgact
     3901 tctggacacg caatgtaaac gatggcaacc ccgtcgagaa attcagcgcc cgcgaactcc
     3961 tgcatagcat ggccgaatcc acatggcagt gcggcgatcc cggcatgcag tatgacacga
     4021 cgattaatcg ctggcacaca tgcaagacca ccgcgcgcat caacgcctcg aacccgtgct
     4081 cggaatacat gttcctggac gacacggcgt gcaacctcgc gtcgctcaac ttgatgaagt
     4141 atctgaattc gaacggttca ttcgacgtgg aagcgttccg ccacgcggta gacgtcacga
     4201 tcacggcgca ggaaatcctg gtggataacg ccagctaccc gacgccgcgc atcgccgaca
     4261 attcgcacaa gttccgccca ttaggcctcg gctacgccaa ccttggcgcg ctgctcatgt
     4321 ccatggcctt gccctatgac tccgatggcg ggcgcgacat ggccgccacc gtcaccgcgc
     4381 tgatgtgcgg agaggcctac gcgcagtcag cgcgcattgc ggagcgcctc gggccctttg
     4441 ccggatacgc cgtcaaccga gagccgatgc tcgaagtgat tcgcatgcac cgcgattcta
     4501 tgcggcccat caagccggaa agcgtgcagc cttcgctgct cggcgccgcg caagcgtctt
     4561 gggactccgc tctttctctc ggcgagaaat tcggctacaa gaattcgcag gtcaccgtgc
     4621 tcgcacccac tggcaccatc ggcttcatga tggactgcga cacgaccggc atcgagcccg
     4681 acctcgcgct tgtgaagtac aagaagctgg tcggcggggg cgtaatcaag atcgtgaaca
     4741 acaccgttcc cgcggcctta atgaagcttg gctacacgcc cgaagagact tcacagatcg
     4801 tttcctacgt cgatcgcaac ggcaagatcg aaggcgctcc cggattcaag cccgagcacc
     4861 tcgcgatctt cgattgctca ctcgcggcgc ccggcggacg ttccatcgcc tggcgtggtc
     4921 acttgaaaat gatggcggcg gcccagccct ttctctccgg cgcgatcagc aagacgatca
     4981 atatgcccga ggaatccacg gtcgaagaca tcatgcaggc gtacatcgag tcatggaagc
     5041 tcggcgtgaa ggccgtcgcg atctatcgtg acaattcgaa gggctcgcag ccgctttccg
     5101 ccgctggcga caagaaggat actgcacagc aaacgcagcc tgccgcttcc gccgccgcag
     5161 ccgccgcgac agccgttgcc gagcagcagg aactcttcgt gcgttcgcgc cgccgcaaga
     5221 tgccaagtga gcgtcaatcg atcacgcaca agttctccat cggcggccac gaaggctaca
     5281 tcaccgttgg catgtatgaa acaggagagc ccggcgaaat cttcatcaag atggccaagg
     5341 aaggctcgac gctctccggc ttcatggacg gcttcgcgct ttcggtttct atcggcttgc
     5401 agtacggcgt cccgctgaag gccctcgtcg ataaatttat caacacccgc tttgagcccg
     5461 ccggctacac cggccatccc gcaattccgt acgcgaaatc cgtactcgat tacctcggac
     5521 gctggctcgg cggccgtttc atttcgcccg actatttaac gcacaacggc gaagctgcct
     5581 tgcccgcagc cacagtaacc gcaatgccag ccgttcctcc ggcatttatc cccacagccg
     5641 atgacgatcc gtccccggcg agcgcacgtt ccacagcaac cgtccgcagc gatgcgcgcc
     5701 ccatgttcga tggcgcgccc acctgctccg agtgcggcat gctgatgacc ccgaacggcg
     5761 cctgctacaa atgcgaaaat tgcggcggca cgtccggctg cagctaaaaa aagcgaagtc
     5821 atcctcagca aaaaacaaaa atggggcgca aacaaagcgc cccattttta tttctcgtca
     5881 cacaaaattt tctccactac ccgtagaaaa tgtgacccgt ctgctgtagc ccgcctcttc
     5941 agaggcgggg gtttaaatta acggtggcga aaacgtaatg gatagcgagt agccgaaccg
     6001 caacgtatgc tcataatggt atctaaaccc ccgcctctga agagacgggc tacagcagcc
     6061 cagctaaaaa ttagcttcgc ctccaaggcg caggccacaa aaagtcttgc ccatccgacg
     6121 aaaaccccac gggatcgacc gatacaggca aatctccttc cccgagataa tgccggtaac
     6181 tagaccacgc ccaatcttcc ttcttcctca ccagtccagc ctgcacagga ttcaaatgaa
     6241 tatattccaa tttttcccaa aaattccgca cccgccgaat aatcgtgtcg aaataccgtt
     6301 cctgccaaag tgggcctctc cgtctcgtgc ctgcacaatc gtataccccg tccgactttt
     6361 aaagttgcgc ataatctgaa tcaaattttt attgtgcgga tgc
//
